LOCUS       DATGKE010000001         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1241956, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000001 DATGKE010000000
VERSION     DATGKE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241956"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VKU83_00005"
     CDS             <1..438
                     /locus_tag="VKU83_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI91955.1"
                     /translation="KTSAVSAFAPCLILKVVAPAVHFYKEAFGAEEVLIVTNEDRTVH
                     VAELAFQGMTFHLHQEMPGSPVKRAPEGATTVELGVFLEDPDSLMDKALAAGAHLVSP
                     MRDYEYGYRQGSFLDPFGHYWTLQKKLASAWKGVIGPQGAPGR"
     gene            525..>2439
                     /gene="rpsA"
                     /locus_tag="VKU83_00010"
     CDS             525..>2439
                     /gene="rpsA"
                     /locus_tag="VKU83_00010"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLI91956.1"
                     /translation="MSENNIVNSNADSQETPVAAPAATTDAAATTKAPVAAQTAHDDF
                     DWSIDKRNVAVYTKEEKSKYDKVYEDTFVAISDGELVKGLVVGVTKTDVVVNIGFKSD
                     GLVSLNEFRDLANLRTGDEVEVMVIEKEDREGHLHLSRKQARITRAWEKIVEVHKTGE
                     VVTGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKINEAIK
                     NAVVSHKALIESDIEAQRAEIISKLEKGQVLEGTIKNITDFGAFMDLGGLDGLLYITD
                     ISWGRISHPSEVLKLDQKLQVVVLDFDDGKKRISLGLKQLTPHPWDVLPPNIKEGEIV
                     KGRVVNIEDYGAFLEIMPGVEGLVHVSEITWANTPINAKEFFKLGDEHNAKVVTLDKD
                     ARKMSLSIKQLSEDPWNTIEVKFPVGSRHNGLVKNITPYGVFVELAPGIGGMIHISDL
                     SWLKRFNHPSEYTRVGHHIDVMILGIDKDNRKLQLGHKQLEEDPWNALEETFAVGSIH
                     EGLVIRRDEKGAIVQLPYGLEGFAPNRHLNKEDGKQIVAEETAPFMVIEFDRNEKRIV
                     LSHTRIWEQVKTEEREALRKEQRADADKTRKAMKNIQSKVEKPTLGDLGALASIKEKL
                     KQEEKDQHSQGGNT"
BASE COUNT          635 a          615 c          714 g          475 t
ORIGIN      
        1 aagacttccg cagtatccgc cttcgcgccc tgcctcatct taaaggtcgt tgctcccgcg
       61 gtgcatttct acaaagaggc gttcggcgcc gaagaagtac tgatcgtcac gaacgaggac
      121 aggacggtcc atgtcgccga gcttgctttt cagggaatga ccttccacct tcatcaggag
      181 atgccgggat cgccggtgaa gcgcgctccg gaaggcgcga caacggtcga attaggcgtt
      241 ttcctggagg atcccgactc gttgatggac aaagctttag ctgccggggc ccatctggtc
      301 agccctatga gggattacga gtatggctac aggcaagggt ctttcctcga tcctttcggg
      361 cattactgga ccctgcagaa gaagctggca agcgcctgga aaggggtgat cgggccgcag
      421 ggcgcgccgg gacggtaaat gcgggaaggc aacggggcat ccaacgggaa ttgagtaatt
      481 tttcgtaatt ttgctacccc gaattaaaat cctcgggatt atttatgtcc gaaaacaaca
      541 ttgttaactc aaacgctgat tcacaggaga caccggtggc cgcgcctgcc gcgacaaccg
      601 acgctgcagc gacaacaaaa gcacctgtgg ctgcgcagac tgcgcatgac gattttgact
      661 ggagtatcga caaacggaac gtagcggttt acaccaagga agaaaagtcc aagtatgaca
      721 aggtgtatga agatactttc gtagccattt cagatggcga actggtcaaa ggcctggtag
      781 taggtgtgac caagaccgat gtagtggtca atatcggttt caagagcgac ggattggttt
      841 ccctcaatga attccgcgac ctcgccaatc tgcggacagg cgatgaggtg gaggtgatgg
      901 taatcgaaaa ggaggaccgg gaaggccatt tgcatctcag ccgtaaacag gcgcggatca
      961 cgcgtgcctg ggagaagatc gtggaggttc acaagaccgg cgaagtcgtt accggcacgg
     1021 ttacgagcaa gaccaaaggc ggcttgatcg tcgatgtatt cgggatggaa accttcctgc
     1081 ccggatcaca gatcgacgtc aaacctgtta ccgattatga tcagtttgtg ggcaagacga
     1141 tggagttcaa ggttgtgaag atcaatgaag ccatcaagaa tgcggtcgta tcgcacaagg
     1201 cccttatcga gagcgatatt gaagcgcagc gcgccgagat catcagcaaa ctcgaaaaag
     1261 gccaggtatt ggagggtacg atcaagaata tcaccgattt tggcgcgttt atggacctgg
     1321 gcggacttga cggtctcctg tatatcaccg atatcagctg gggacgcatc agccatccga
     1381 gcgaggtcct caagctggac cagaagctgc aggtcgttgt gttggatttc gacgacggca
     1441 agaagcggat cagcctcggc ctgaagcagc tcacgccgca tccctgggac gtattgcccc
     1501 cgaatatcaa ggaaggcgaa atcgtcaagg gccgcgtagt caatatcgaa gattacggcg
     1561 ccttcctcga aatcatgccg ggcgtcgagg ggttggtaca cgtaagcgag atcacctggg
     1621 caaacacccc gatcaatgca aaagagttct tcaaacttgg cgacgagcat aatgcgaagg
     1681 tcgtgaccct cgataaggat gcccggaaga tgagcctttc gataaagcag ctgagcgagg
     1741 atccgtggaa tacgatcgaa gtcaaattcc ccgtcggcag ccgccacaac ggactggtca
     1801 agaatatcac tccctatggg gtattcgtgg agctggctcc cggcatcggc gggatgatcc
     1861 acatcagcga tctcagctgg ttgaagcgct ttaaccatcc gtccgaatat acccgggtcg
     1921 gccatcatat tgatgtgatg atccttggga ttgataagga caaccgcaag ctgcaactcg
     1981 ggcacaagca actcgaagaa gatccctgga atgcattgga ggagactttt gcagtcggct
     2041 ccatccatga aggtctggtg atccgcaggg atgaaaaggg tgcaatcgtg cagctgccat
     2101 acgggctcga gggtttcgct cccaaccgcc acctgaacaa ggaagacggc aagcagatcg
     2161 tggccgaaga gaccgctccc ttcatggtga tcgagttcga tcgaaatgaa aagcgcatcg
     2221 ttctatccca tacccggatc tgggaacagg tcaaaacgga agagcgcgaa gcgctccgga
     2281 aggaacagcg ggctgacgcc gataagacgc gcaaggctat gaagaacatc cagagcaagg
     2341 ttgaaaagcc gacactcgga gacctcggcg ccctcgcctc catcaaggag aagctgaaac
     2401 aggaagagaa ggatcagcat tcgcaaggcg gcaacactg
//
LOCUS       DATGKE010000002         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1241758, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000002 DATGKE010000000
VERSION     DATGKE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241758"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VKU83_00015"
     CDS             complement(<1..1129)
                     /locus_tag="VKU83_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLI91957.1"
                     /translation="MERPIYINKYLPVAVLYFFFNSVFLPLGLLYTSILAPFFLFWIH
                     YQKRPSGLWHFLTLSLLFACVHFYNGVNPGYYLKSYLILLATFIFCVAFYVFVSKVRD
                     LRRLFKDLLLINTVLVLLALIFLAIPPLRNIVWYSNAITTGVKNLLRLRLFTYEASYY
                     STLLVPLVLYYCLKVLILKSPQKWPVLLMATIPILLSLSFGVILALIAAIVAVYLSDL
                     RLITENRKAIRSIVFFAALVVLGLIILYKISPGNVVFKRLANVFEGRDTSFKGRTTDS
                     FFIAWQIAMKRSIFFGCGPGQVKALGVEILRHFYRSNNYTVANTAIPNVLAETLAVYG
                     LIGLFLRLGAEVYLFFRTKVYRNYYRLSLFLFIFIYQFTGSY"
     gene            complement(1141..2067)
                     /locus_tag="VKU83_00020"
     CDS             complement(1141..2067)
                     /locus_tag="VKU83_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91958.1"
                     /translation="MTDPRYYSEDYISLDHIKAFFYSFVKGLFSVVDSASGLIVKYWM
                     LFLVLVVMGTGVGYLLKSVVTYNSQLFMLVKFNDLDPSVYLGMINSVNDQAKSKAYAR
                     LATELGLKQEDAQQITEVSLDKPGKETWSPEDSSASRTPFMIKITLRSITAADAVQSA
                     IMAYFNENAYIKSLKEGQLAYYQDELVFINSELRKLDSLKSEYNRSLTAGKNPTIYYN
                     AFNPADIYGRSATLMDRKENLANWFATQEHAASLISGIKQEKVAAKRSFILVVWGFLA
                     GVLLSFWIAIWRELRIRIAADKQDQPGPSAGK"
BASE COUNT          640 a          535 c          597 g          530 t
ORIGIN      
        1 tatagctgcc ggtgaactga taaatgaaaa tgaacaggaa caggctgagc cggtaatagt
       61 tcctgtagac ttttgtccgg aaaaacaaat agacctcggc gccgagccgc aggaataagc
      121 caataagtcc gtaaacggcg agggtctccg caagcacgtt agggatcgcg gtgttggcga
      181 cggtgtagtt attggaacgg taaaaatgcc ggagaatctc cacgcccagg gccttgacct
      241 gacccgggcc gcagccgaaa aagatgctcc ttttcatggc gatctgccag gcaatgaaaa
      301 atgaatcggt tgtcctgccc ttgaacgagg tgtcgcgccc ttcgaacaca ttggccagcc
      361 tcttgaaaac aacgtttccg gggctgatct tgtagaggat gatcagcccg agcacgacga
      421 gcgctgcgaa aaatacaatg cttctgatcg ctttcctgtt ctccgttatc agcctgaggt
      481 ccgacaaata caccgcaaca atcgcggcga tcagggccag gatgacccca aaggaaagtg
      541 ataagaggat aggtatggta gccatcagca agaccggcca tttctggggc gatttaagga
      601 tcagcacctt caggcaataa tagagcacga ggggtaccag aagcgtagag taataagagg
      661 cctcgtaagt gaaaagcctt agcctgagca gattctttac gccggtcgtg atggcattgc
      721 tataccatac aatgttcctc aatggcggta ttgccagaaa gatcagcgcg agtaacacca
      781 aaacggtatt gatgagcagg agatctttga ataaccggcg caaatcgcgg accttggaaa
      841 caaaaacata gaatgctacg cagaatataa aggtcgccag caggatgaga tatgacttga
      901 gataataccc gggattcacc ccgttgtaaa aatggacaca ggcgaacaat aatgacaggg
      961 tcagaaaatg ccataaaccg gagggccttt tttgatagtg gatccaaaaa aggaaaaagg
     1021 gggccaggat agaagtataa aggagtccga gtggaaggaa aacggagttg aaaaaaaaat
     1081 acaataccgc aacaggcagg tatttgttga tataaatggg tcgttccatt tccttgatgg
     1141 ttatttgcct gctgacgggc cgggttgatc ctgcttatcg gccgctatcc tgattctaag
     1201 ctcccgccaa atagcgatcc aaaagcttag caacaccccg gcgaggaagc cccaaaccac
     1261 aagaatgaat gagcgcttcg ctgcaacctt ttcctgtttg atcccgctta taaggctcgc
     1321 cgcatgctcc tgcgtggcaa accaattggc caaattttcc ttgcgatcca tcagggtggc
     1381 tgatctgcca taaatgtccg ccggattgaa tgcattgtaa tagatggtcg gatttttgcc
     1441 tgcggttaaa ctccggttat actctgactt gagggagtcc agtttccgga gctcgctgtt
     1501 gataaagaca agctcgtcct ggtaataggc cagttggcct tcctttaggc ttttaatgta
     1561 agcattctcg ttgaaatagg ccatgatggc ggattggacc gcatctgccg cggtgatgct
     1621 ccggagcgta atttttatca tgaatggcgt tctggatgcc gagctgtcct ccggcgacca
     1681 ggtttcctta cccggcttat ccaggctgac ctcggtgatt tgctgcgcat cttcctgctt
     1741 taaaccaagt tctgtagcca gccgggcata agccttcgat ttggcctggt cattcaccga
     1801 attgatcatc cccagataga cggaagggtc aaggtcgttg aatttcacca gcatgaaaag
     1861 ttggctgtta taggtgacga cgctcttcag cagatagccg acgccggtac ccatgacgac
     1921 caggaccagg aagagcatcc aatatttcac gattagtcct gaggcggaat cgacgacgga
     1981 aaaaaggccc ttcacgaaac tgtagaaaaa ggctttgata tgatcgagag aaatataatc
     2041 ttcggaataa tacctgggat cagtcatatt ggatgccatt taaaattgga tggttgcgtt
     2101 gccgcaaaac taaacagcac aataataaga ataattttgc aagaccctta aaaacggttc
     2161 cctgctaagt agttgattat tacctcggcg atgcgtttta tcggccctat cgaataggac
     2221 agaccggtac ttccggagat gccgccgaaa agtggcggga cgccgaatcc ggtataggac
     2281 ccaggctgct gctttaatgc ct
//
LOCUS       DATGKE010000003         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_621011, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000003 DATGKE010000000
VERSION     DATGKE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621011"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1151
                     /locus_tag="VKU83_00025"
     CDS             99..1151
                     /locus_tag="VKU83_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HLI91959.1"
                     /translation="MAGRVLLWMIAGVVTVGKVRAQISANGVNYVNTYKALAIEEEQR
                     TGVPAAITLAQGLHETEAGTSELVRNSNNHFGIKCKADWKGPVYYHDDDARGECFRVY
                     ATAADSYRDHSDFLRQGARYAFLFELDPADYEQWAYGLKKAGYATNVRYSQILIKLIR
                     DYHLEQYTLIATGKMAASDEVVLTAPGSGEASAAATPNAIVASTGLLPETSRDSTGSA
                     YPGGEFMINRLRVVYVPAGASLLSVAIKYDVPLGRLYEFNEMQQEEVLAKGQLIFLQR
                     KRNKGATAYHVVKPGEDLYGIAQAEGIRLADLLEMNQLSQGAQPEAGEKVYLQHPAPE
                     RPKLVVKTDYVDHKGA"
     gene            1129..1665
                     /gene="hpt"
                     /locus_tag="VKU83_00030"
     CDS             1129..1665
                     /gene="hpt"
                     /locus_tag="VKU83_00030"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLI91960.1"
                     /translation="MSIIRVHDKNFEPYLSAERIAEKIREVAGRLNRDYEGKKPLFLA
                     ILNGAFMFASDLFKEIKIEAEISFIKLASYKGTKSSGHVITAIGLDIDLIGRHVVIVE
                     DIVDTGKTLSEFLPQLEHQQPASMKIAALLHKPEATVYPIQVDYLGFSVPNKFLLGYG
                     LDYDGLGRNIPSIYHLVE"
     gene            1720..>2790
                     /locus_tag="VKU83_00035"
     CDS             1720..>2790
                     /locus_tag="VKU83_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HLI91961.1"
                     /translation="MKRAFLLAMASLLLHAARAQYYLRGEIRDENNALMSNVKMRLHS
                     NGYLYYSGISGAFGIPIPRALDTLTLDAEGYEEQTVGVDANIYQTIRLKPIFRAQSGV
                     VQARGLMSFTKDWRPMDHQDWTVGAETYSSLVENSFIPAAKYPETGFAIRIDKASYSN
                     VRRFLNMSSTIPPDAVRIEELLNYFNFGYTDPPPDSCFLLRSRLSECPWNPDNELLFL
                     QVCARKLDPSKIPPANLVFLIDASGSMDLPNKLPLLKSAFSLLVNNLRPIDTVTIVTY
                     GNLVGVWLPPTSGAEKKKILKSIDELTPGGATPGEAGIRAAYRVAKSQFIKGGNNRVI
                     LATDGDFNEGETSEEELGRLVSQ"
BASE COUNT          654 a          702 c          850 g          584 t
ORIGIN      
        1 acaggccttc aatgagacgg tagcggcgga cgccaccgtc gagagggtaa tgctcaccgt
       61 aagggatggg ctatttctca tttacaaaaa atagactgat ggcaggcagg gtgttgttat
      121 ggatgatcgc cggggtggtt accgtgggca aagtgcgggc ccagatcagc gctaatggcg
      181 taaactacgt gaacacctat aaggcccttg cgatagaaga ggagcaacgt acgggagttc
      241 cggcggcgat cacgctggcg caggggctgc acgagacgga ggcggggacg agcgagctcg
      301 tccggaactc caacaatcat ttcggaatta agtgtaaagc ggactggaag ggtcccgttt
      361 attaccatga cgatgatgcg cggggcgaat gtttccgggt ctatgcgacc gcggcggatt
      421 cataccggga ccattcggat ttcctccggc agggcgcaag gtatgctttc ctgttcgagc
      481 tggacccggc ggattatgag cagtgggcct atggcttgaa aaaggccggg tacgcgacga
      541 acgtgcgtta ttcccagatc ctcatcaagc tgatcaggga ctatcatctc gagcaatata
      601 cgctgatcgc gaccggtaaa atggcggcgt cggatgaggt ggtcctgaca gctccgggca
      661 gcggcgaagc ttcggccgcc gcaaccccga acgcgatcgt cgcgtcaacc ggattgctcc
      721 cggaaacttc acgagactcg acaggatcgg cttatcccgg gggagaattc atgatcaacc
      781 ggttgagggt ggtctacgtg cccgcgggcg cctccttatt gtcggttgcc ataaaatatg
      841 acgtgccatt ggggcgcttg tatgaattta acgagatgca acaggaagag gtgctggcaa
      901 aggggcaatt gatctttttg cagcggaagc ggaacaaagg ggcgaccgcg taccatgtgg
      961 tcaagccggg agaggacctg tatggcattg cgcaagccga aggaataagg ctggccgatc
     1021 ttttggaaat gaatcaattg tcgcagggcg cccagcccga ggctggcgaa aaggtttact
     1081 tgcagcatcc ggccccggaa aggccgaaat tggtggtaaa aacggactat gtcgatcata
     1141 agggtgcatg acaaaaattt tgagccttat ttgtcagcgg aacggatagc ggagaagatc
     1201 agggaggtgg ctggccggct gaaccgggat tatgagggaa agaagccttt attccttgca
     1261 atcctgaacg gagccttcat gtttgccagc gacctgttca aggaaatcaa gatcgaagcg
     1321 gagatcagct tcatcaagct ggcttcctat aaaggaacga agtcttccgg gcacgtgatc
     1381 acggcgattg ggctggatat agatctgatc gggcggcatg tggtcatcgt cgaggacatt
     1441 gtggataccg ggaagaccct gagcgaattt ctgccgcagc tggagcacca acagccggca
     1501 tcaatgaaga tcgcggcact gctgcacaag cctgaagcga ctgtctaccc gatccaggtg
     1561 gattacctcg gcttttccgt ccctaacaag ttcttgttgg gttacggtct cgactacgac
     1621 ggattagggc ggaatatccc gtcgatttac cacttggtgg aataaagcag gggcttgaca
     1681 aataggaaag aacgcattaa ctttaatcaa aatatccgat tgaaaagggc atttttgctg
     1741 gcaatggctt ctctgctgct gcatgctgcg cgagcgcaat attacctgcg tggggagata
     1801 agggatgaga acaacgccct catgtcgaat gtgaagatgc gtctccactc caacggctac
     1861 ctttactatt cgggtatctc gggggctttc gggattccca taccgcgggc gctggacacg
     1921 ctgaccctgg atgcggaagg ttacgaggaa cagacggtgg gggtagacgc gaacatctac
     1981 cagacgatcc gcctcaaacc gatcttccgg gcgcagtcgg gagtagttca ggcgcggggc
     2041 cttatgtcct tcacgaagga ttggcgcccg atggatcacc aggactggac ggtgggggca
     2101 gagacctaca gttcactggt ggaaaatagt tttatccccg cggcgaagta cccggagacg
     2161 gggtttgcga tccggatcga caaggcctcc tacagcaatg tcaggcgctt cctgaatatg
     2221 agcagcacca ttccgccgga tgccgtccgg atagaggaac ttctcaacta tttcaacttt
     2281 ggctataccg atcccccgcc cgacagctgc tttctgctcc ggtcgcgctt atccgaatgc
     2341 ccgtggaacc cggacaacga gttattattc ctccaggtat gcgcgcggaa gcttgatccc
     2401 tcgaagatcc cgccggccaa cctggtcttc ctcatcgacg cctccggttc aatggatctg
     2461 cccaacaagc tccctttgct caagtccgcc ttcagccttt tggtgaacaa tttgcgcccg
     2521 atcgataccg tcactattgt gacttacgga aacctggtgg gagtttggtt accgcctact
     2581 tcaggggcgg agaaaaagaa gatcttaaag tccatcgacg agctcactcc gggtggcgca
     2641 acgccgggag aggcgggcat ccgcgcggcc taccgggtgg cgaaaagcca gttcatcaag
     2701 ggcgggaata accgggttat tctggcaacg gatggcgact tcaatgaagg ggagacgtcc
     2761 gaagaggagc tggggcggct ggtttcgcaa
//
LOCUS       DATGKE010000004         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1240850, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000004 DATGKE010000000
VERSION     DATGKE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240850"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..833
                     /locus_tag="VKU83_00040"
     CDS             219..833
                     /locus_tag="VKU83_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI91962.1"
                     /translation="MDQLSDILKSARNSKGFSQQYVADQLGISQRAYGFYEEGRRTPK
                     WPKLKKIASILGLDQNEMLRMHAQILELDVTNSTAGEGGEEEPTAREILMEMARARTA
                     HEKSYDTLARIIERQTSILELIKSDMARQSSLDDIKANLKDLAEGREDLEANVFAAIH
                     RASERFLGTEDPDKVKIEIDKISRLAASYHAVNLKKGKLARRVR"
     gene            1140..1769
                     /locus_tag="VKU83_00045"
     CDS             1140..1769
                     /locus_tag="VKU83_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91963.1"
                     /translation="MKKIPFLFFLFSPLWLFPQTGTIAGSAFWKYNDFIGNKADAASE
                     VWLYRDSVAAPLKTECDFQGSFKFDNLKPGKYLIIIKSKNAKDDWSDNYIQLRYAPLS
                     EYFTKDISSIDKSLFDSATYYQKAYSSATTEKYGPFSANKHFKKMRELNRQYLQCVKR
                     VFSNIPKGSFQYFDWEIPLDYPHRLFIKECEVKPNETFSVVADFGLTAI"
     gene            complement(1775..1924)
                     /locus_tag="VKU83_00050"
     CDS             complement(1775..1924)
                     /locus_tag="VKU83_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91964.1"
                     /translation="MHIRTLGSRIKDPGKWHVHELAALSDLLEIDHAVLLQMADKLRK
                     KKGKK"
     gene            2263..2952
                     /locus_tag="VKU83_00055"
     CDS             2263..2952
                     /locus_tag="VKU83_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLI91965.1"
                     /translation="MNLKRIALISIGLNIMGLLLFAGRKTIYRPQPPKTESTDAYYDK
                     WNQARKSVLDMLPIDSADIVFVGNSITEGFPLQEMFPGIHVKNRGIGGNQSRHILGRI
                     QEIAAGHPRKIFFDIGVNDLRAHIPVDSILHNYARIIEAVHFLSPRTVIYVQSVFPIG
                     PSYKEIKDSVPAFNLELRHECHGWQVNFIDLYPLFLQGAYIRPTLTFDDLHLTGAGYA
                     LWRQAIVGYLP"
     gene            complement(2955..>3677)
                     /locus_tag="VKU83_00060"
     CDS             complement(2955..>3677)
                     /locus_tag="VKU83_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLI91966.1"
                     /translation="LKAFAGEYLEWKEITTQFLRNFEHHERKRGMAQNTLNTSFRWMK
                     AILNKARKEGLLKELPSYVTPKYLQPERVFLVEEQRQDWLKYWREKKCDGSTYNTLTW
                     FLFGCYTGLRHSDWEQFDYEQRVQGEFLKLRAKKNGRWVVLPIGKTLSEIINVVKDLP
                     KPLSGDKTRAYLKVLAGRIGEPRTVTTHTCRHSFGAMCAQLRLPKSTAAELMGVTEKV
                     VSVYYHLTGVDIIEQAAALRDV"
BASE COUNT          992 a          844 c          855 g          986 t
ORIGIN      
        1 taagcgccgg atcattgtct gagatgtatc tgtacatagt ctgcttagat actcccaggg
       61 cctccctggc ggcttgccag attggtttat ttcccctgag tatcgatatg gctgctatac
      121 ttagcttcat attttcttat atttgagaat ctgaagtaaa gataagaaca tttagttcta
      181 aaaaacaaat tttagtaaca ttttttactt aaattgattt ggatcaatta tcggacatat
      241 taaaatcggc ccgaaattca aaggggtttt cgcagcaata cgttgctgat caattaggta
      301 taagccagcg cgcgtatgga ttttatgagg aaggcagaag aacgccgaag tggccaaagc
      361 ttaaaaagat agcctccatt ctcggtttgg accagaacga aatgttacgc atgcatgccc
      421 aaattttaga actcgatgtt accaattcca cggccggaga gggaggggaa gaagaaccaa
      481 ccgccagaga aatattaatg gaaatggccc gcgccagaac cgcacatgaa aaatcctacg
      541 acaccttagc gaggattata gaaagacaga catctatatt ggagcttatc aagagcgata
      601 tggctaggca atccagcctg gatgatatta aggctaattt aaaagacctt gctgaaggtc
      661 gagaggatct tgaggcgaat gtgttcgcgg ccattcatcg ggcatctgaa agattcttag
      721 gtaccgagga cccggacaaa gtgaagattg agatcgacaa gataagcaga ctcgctgcct
      781 cctatcatgc ggtaaatctg aaaaagggca agctggctcg cagggttcgt taataatagg
      841 cgattccata aaggtctagg tcttttaacg tggttaaaaa gattgttggt taggaggttt
      901 gatggaacgg ctgaaaagga caaggaagca gggtagcggg gtaacgaact tagaaaagtt
      961 atccaatcag tccaaatttt tgtagaaata ttatgatgga ttttacgttt ttacatggag
     1021 atacttagta agagtaaaat tgtcagcgag cacgagtaaa cccctatatt gcacacttat
     1081 cgcccaaatc tacacggcta tgagcaaatt cgacacggtc aataattaaa ccagacgata
     1141 tgaaaaaaat cccctttctc ttttttcttt tttctccctt atggctattc ccccaaacag
     1201 gcaccattgc cggaagcgct ttttggaaat ataatgattt cattggtaat aaggcggatg
     1261 cggcaagcga ggtttggtta tatagggatt ctgttgcggc gcccttaaaa acggaatgcg
     1321 actttcaggg cagcttcaaa tttgataatc ttaagcctgg aaaatatttg ataataataa
     1381 aatccaagaa tgcaaaagac gattggtctg ataattatat ccagcttcga tatgcgcccc
     1441 tttcagaata ttttactaag gatatttcat ctatagacaa atcattgttc gacagcgcaa
     1501 catactatca aaaagcttat agttcagcga cgactgaaaa gtacggcccc ttttctgcga
     1561 ataagcattt taaaaaaatg cgagaattga atcggcaata tctacaatgt gttaagaggg
     1621 ttttctccaa cataccaaaa ggaagttttc aatattttga ctgggagata ccgctggatt
     1681 acccccatag acttttcata aaagaatgcg aggtaaagcc aaatgaaaca ttttcggtag
     1741 ttgcagattt tggcctgacg gcaatataat caaatcactt cttccccttc ttcttcctga
     1801 gcttgtcagc catctgcagc aggacggcgt gatctatctc caggagatcc gataaggcgg
     1861 ccagctcatg gacatgccat ttgccggggt cctttatgcg cgaaccaagc gttcgtatat
     1921 gcatgcccat gtctttggcc aaggtctttt tctgtatgtc agaggctatt tcttcgaaat
     1981 ctcggatatg gccgtcgcgc aggagtatac cgaccttccg ataggctgga cttttcatcc
     2041 ccgcaataac ggtcagtgat gcaatcaatt attagtacga cacttaaact accatagata
     2101 gttccactta tgccacagaa aaagcactta gatgccattg cgatgtcaaa aaaatgacag
     2161 taccttggca ataataggat aaggtttgat ggtgatggtt ccgattagat ggcagttacg
     2221 tcggctcttc catggtggga ggagcctttt ttaaatcttt ctatgaatct aaaacgcatt
     2281 gctctcatca gcatcgggct caacattatg ggcctccttc tctttgcagg caggaaaaca
     2341 atctatcgcc cgcagcctcc gaaaacggaa tccaccgacg cttactatga caaatggaac
     2401 caggcaagaa agtcggttct ggatatgctg ccaattgatt cagccgacat tgtttttgtg
     2461 ggcaattcga tcacggaagg gtttccgttg caggagatgt ttccaggcat ccacgtaaaa
     2521 aacaggggta taggcggcaa ccaatcccgc cacatattgg gtcgcattca ggagatcgcg
     2581 gccggtcacc cccgaaagat attctttgat atcggggtga atgatcttcg ggcgcacatc
     2641 cccgtcgaca gcatcctgca taattatgcc cggatcatcg aagcagtaca cttcctgtct
     2701 ccgcggacgg ttatttatgt gcaatccgtc ttcccaattg ggccgtcgta caaagaaatt
     2761 aaagactccg tcccggcgtt caatctcgag ctacgacacg aatgccacgg ctggcaggtg
     2821 aacttcattg acctgtatcc gctttttctt cagggggcat acattcggcc cactctcacc
     2881 ttcgacgatc tgcatttgac gggcgccggt tatgcccttt ggcggcaggc gattgtgggt
     2941 tatttgccat agccttatac atctcgcagc gctgccgcct gttcgatgat atctacgccc
     3001 gtcaggtgat agtacacgct caccactttt tcggtgacac ccatcaattc tgcagcagtt
     3061 gatttcggca ggcgcagctg ggcacacatc gccccaaatg aatggcgaca tgtatgggtg
     3121 gtgaccgttc tgggctcgcc aatccgaccc gccaatacct tcaggtaagc ccgggtctta
     3181 tccccggaca ggggttttgg aaggtctttc accacattga ttatttcgga gagcgtttta
     3241 ccgatgggca gtaccaccca ccggccattt ttcttggcgc ggagtttcag aaactccccc
     3301 tggacgcgct gctcataatc gaattgctcc cagtccgaat gacgaaggcc ggtataacag
     3361 ccaaacaaga accacgtcag ggtattgtag gtggatccgt cgcacttttt ttcccgccag
     3421 tatttcagcc agtcctgccg ctgttcctcc accaggaaga cccgttcggg ctggaggtat
     3481 ttaggtgtta cgtacgatgg gagctctttt aacagcccct ctttgcgggc tttgttcaat
     3541 atcgccttca tccatcggaa ggacgtattg agcgtatttt gagccatacc gcgcttccgc
     3601 tcgtggtgct cgaagttgcg gaggaactgc gtggtgattt ccttccattc caggtattcc
     3661 ccggcgaagg ctttcag
//
LOCUS       DATGKE010000005         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1451774, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000005 DATGKE010000000
VERSION     DATGKE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451774"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2054)
                     /locus_tag="VKU83_00065"
     CDS             complement(105..2054)
                     /locus_tag="VKU83_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91967.1"
                     /translation="MKKHFGCLVALLLIALQFSWHSVNGQYEYVRSVSIVPDSKAYWE
                     YLPVGYNSKDSLKYPLILYIHGAGDYGNGTPTSMKLVLRAGPLRYISLNQFPQTFTVN
                     GKTYGFIVIAPQFMLQPSVTDINSVINFCIATYKVDVSRIYLTGLSMGGALSWEYAGN
                     QLAYANRLAAIVPVCGETPPDYNRGRVIAASNLPVWATHNDGDPLAPVQWSIQQVAWI
                     NEPPPPNPLAMLTIFHGNTHDAWTTTYNPAFTLNNLDIYQWMLTYSRASAPPVYQPPE
                     VTAGPDQSITLPVSTVNLTGSATASSGTITAHTWTFVSGPATPAIAAPSNYATAVTGL
                     TVAGSYVFRLSATDSHNLTASANTQVIVYAAAADTGRVINVNLYGNSDPYNSGAWNNW
                     NVGSGSLGSAKFNYSNGSPSAVSAALSAQTAVADNGASYNVTMCPQKVARYASYYSGA
                     GGRTLTISGLDMTKLYRVDIYATRNNPLQTTTFGTGGTNVTIATNNNTSTVASFENLS
                     PKTGGQVVITMTHSQYYDYINGFTITEKTPLGPENLDSALETAVPLIITLALDSSSTI
                     DQPLSLYPNPTAGPVELHINNSLIGRMKVDIYSEAGTLVREYVLTKTTAKMDELLSLD
                     GLTRGGYILVATIGNWQHNLRLLKL"
     gene            2391..>3107
                     /locus_tag="VKU83_00070"
     CDS             2391..>3107
                     /locus_tag="VKU83_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91968.1"
                     /translation="MLSKLTACFTMLLIGIAANAQQKGYYRTPAIWHQTIVFTAEGDL
                     WKYDLTSGITARLTTNEGMETNPVISPDGRSVAFTGQYEGSNEVYVMPLEGGVPKRLT
                     YDLDGSARPTDWLKDGRLLYATAAYSSYPIPQLIKIDPNTLATDQVPLFEAADGVYDG
                     NGVLFFTRLPNQGSKTKRYKGGFIEQIWRFDGRTEAVNLTADFEGTSSHPMLYNGRVY
                     FLSDRDGTMNIWSMDENGKDL"
BASE COUNT          730 a          752 c          902 g          723 t
ORIGIN      
        1 aaacttgggg gcgaggaggc cgctgttcgg ctaaccgggt acaaattgag tttatcgcca
       61 agcggacagg cggatgggaa tgaaaagaag ggtacggact aaatttatag tttcaacagc
      121 cttaggttat gctgccagtt gccgatggta gccacgagga tatatccgcc ccgggtcagg
      181 ccatcaaggg acaacagctc atccattttg gctgtcgttt ttgtaagcac atattctctt
      241 accagggtac cggcttcgga atagatgtct actttcattc ggccgatgag agaattgttg
      301 atgtgcaact cgacgggacc ggcggttgga ttaggataca gactgagcgg ctgatcaata
      361 gtcgagctgc tatccagagc aagggtgatg atcaaaggga cggcggtttc caaagccgaa
      421 tccaggtttt ccggtcccag gggcgttttt tccgtaatgg tgaagccgtt gatatagtca
      481 taatactgtg aatgggtcat ggtgatgaca acctgcccac ccgttttggg actgaggttt
      541 tcgaaactgg ccacggttga ggtattgtta ttcgtagcga tggttacatt tgtgccgccg
      601 gtcccgaacg tcgtggtttg caatggatta ttacgagttg catagatatc gacacgatat
      661 agcttcgtca tatccagccc gctgatcgta agcgtgcgac ccccggcgcc cgaataatag
      721 ctggcatatc gggcgacttt ttgcgggcac atggtgacat tgtagcttgc tccattgtct
      781 gcaacggctg tttgagcgga gagagcggcg gaaacagccg atggagaacc gttcgaataa
      841 ttgaattttg ccgagccgag gctcccgctg ccgacattcc aattattcca agcgccgctg
      901 ttgtaggggt cactattacc gtagaggttt acgttgatca cgcggcccgt atcggcggct
      961 gcagcataaa caatgacttg tgtattggcg gatgccgtca ggttgtgact gtctgttgcc
     1021 gaaaggcgga agacatagct ccccgcgacg gtcagcccgg tcaccgcagt ggcatagttg
     1081 gagggcgctg caatcgcggg tgtcgcgggt cccgagacga aagtccaggt atgggccgtg
     1141 atggtcccgg aactggcggt ggcggatccg gtcaggttaa ccgtcgagac aggcaaggta
     1201 attgactggt ccggtccggc agtgacctca gggggttggt ataccggtgg agctgacgcg
     1261 cgcgaatacg tcagcatcca ttgatagata tccagattat ttaaggtaaa agccgggttg
     1321 taagttgtcg tccaggcatc gtgcgtattc ccatggaaga tggtaagcat ggccaggggg
     1381 ttgggaggcg gcggttcgtt gatccatgcg acctgctgga tggaccattg aacgggggcg
     1441 agtgggtctc catcattatg cgtggcccac acggggaggt tggatgccgc tatcacccgg
     1501 ccgcggttgt agtccggcgg ggtctcgccg cagacgggaa cgatagccgc caaccggttg
     1561 gcataagcca gctggttgcc tgcatactcc cagctcagag caccgcccat gcttagtccg
     1621 gtaaggtaaa tacgggacac gtctacctta taggtagcga tacaaaaatt gatcacactg
     1681 ttgatgtcgg taacgctggg ctgcaacatg aattgcggcg ctatcacaat gaacccgtat
     1741 gtcttgccat tcaccgtgaa ggtctgcgga aactgattca ggctgatgta ccttaaagga
     1801 ccggcacgca gaaccagttt cattgatgtg ggtgtgccat tgccgtaatc acccgcgccg
     1861 tggatgtaga ggatcaacgg atacttcaag gaatcttttg aattatatcc aaccgggagg
     1921 tattcccaat acgctttgga gtcgggaacg atcgaaacgg accggacgta ttcatactgg
     1981 ccgtttacgc tgtgccagga aaattggagc gcaatcaaca acaacgcaac cagacacccg
     2041 aaatgttttt tcatgccaga aagcttttaa gacgtgattc caggttcacc ttttatgcga
     2101 actcaggcgt ccaggatcat tttcagtgta cttgattggg ttgctgacgc ggtggtctcg
     2161 ggcgatgaac cgtcaatgct tcccggtcag atagaggttg gactgatgtc ataagttatg
     2221 ccttgttggg gaccgacatt gcatcttaaa tcgtcactac acaaaacacg acattccgcc
     2281 cgataaaatc ggtgacagtg atcacccatt ggtttgattc ccgtcagtcc gtcagcagga
     2341 ttgtcaaatt gcggcatttt ctttacattc agcgcctaaa tctctccgcc atgttatcaa
     2401 agctgactgc ctgcttcact atgctgctga tcggaatcgc tgcaaacgcc cagcagaaag
     2461 gctactaccg gacacccgcg atctggcatc aaacgatcgt attcaccgcc gaaggcgacc
     2521 tttggaaata tgatctgacc agcggcatca cggcgcggct gacaacgaac gaggggatgg
     2581 aaaccaaccc ggtgatttcc cctgacgggc gaagcgtggc cttcacgggg cagtatgagg
     2641 gctcgaacga agtgtatgta atgccgctgg aaggcggtgt gccgaaacga ctcacctatg
     2701 atctcgacgg tagcgcgcgg ccaaccgact ggctaaaaga cggccggctg ctttacgcga
     2761 ccgcagccta tagcagctat ccgattccgc aattgataaa gattgatccg aatacgttgg
     2821 cgaccgatca ggtgcccttg tttgaagcgg ccgatggcgt gtatgacggg aatggagtgc
     2881 tgttttttac gcgcctccct aatcagggca gcaaaaccaa aagatacaaa ggtggattta
     2941 tcgagcagat ctggcggttt gacggcagaa cggaagcggt caacctaacg gcagattttg
     3001 agggcaccag cagccatccc atgctgtata acggccgggt gtatttcctt tctgaccggg
     3061 atggaaccat gaatatctgg tcgatggatg agaacggcaa agacctg
//
LOCUS       DATGKE010000006         5242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_930220, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000006 DATGKE010000000
VERSION     DATGKE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930220"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..781
                     /locus_tag="VKU83_00075"
     CDS             152..781
                     /locus_tag="VKU83_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI91969.1"
                     /translation="MIKIAIVEDNNTLRSSLESLFNRTDGMRCVASLNNLLNVVGEIG
                     SALPDIILMDIGLPNISGIEGVRTVKTHFPAIQILMFTVFDDDEKVFDAIRAGASGYL
                     LKKTPAAEIIDAIRELYNGGAPMTSSIARRVIQSFQAAPSSVIKDYQLTVRENEILYS
                     LVDGLSYKKIADKYCVSISTIRTHICNIYHKLHVNSKAEAVARVLGPHK"
     gene            904..2160
                     /locus_tag="VKU83_00080"
     CDS             904..2160
                     /locus_tag="VKU83_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI91970.1"
                     /translation="MKNFCTAATAALLLAAIVAPTPRACAQVTNPGTTAGQSATDHVN
                     NNVSNGINNGLDKTENSLKGLFKKKNKAPKNDTAKASASSTSHSTANASGPDGQNAPT
                     SLKAYSNYDFVPGEQVVFEDHFTDDQDGEFPAHWKLTSGQGVTNKVGDEPAFFLTEGN
                     YVRVAPRMKAEKNYLPDNFTVEFDFYPTEGSYYPMLLFTTATDDSRFIQFGDEVATSY
                     FPSDFSAQYPGDRDNFNGKWHHAAMIKKGNQIKCYEDQYRVLVIPDCGGCKMSFVEVG
                     GIGDKDHPIVFDNFRLAEGGNMNLIGQKFTDAKLVTHGINFDVDKSSIRPESMGTLNM
                     VAGILKSNPDLKFEIDGHTDNSGDAAHNLTLSLQRAAAVKAQLVAMGIDGSRLTAKGF
                     GDTKPISDNSTLEGKANNRRVEFVKM"
     gene            2189..4321
                     /locus_tag="VKU83_00085"
     CDS             2189..4321
                     /locus_tag="VKU83_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91971.1"
                     /translation="MRKLFLSALLCCSGLLPGLSLRAQAPAPATGGVGLWDAHHFLWF
                     TGHWNKSGSRFRFIVDRGNTATTGAGGGGAAPGTTGDNDDPPGNPPDGGTITIHTDGT
                     VNLDNSGVTEWNKKMDEALKSEEDYLRALQDPGDNLDPTTHHLHQLLVPDARQRVNDI
                     QTYKIGADQDMLGADSKSAPPQRTLSDAAEDLCRDIKPDYDLIMNYYKTQVKGHNNDL
                     RFPAPPTADYDCYSCDSSVRKLYDTAIYHYADNFGKEEDDIVMKGLAILHKLGLMGVS
                     TESGYSPELDNAFRKNKQDPSKAGACSFIELTYFSDAVYAIAHHLYLRAEKMVYANKN
                     NYKAAEAIIRAYLKQARAWAMRSGNTEGNDMLPLLASLAGQAVDFYIKAFRHDDWRQV
                     GNATLILQMIRDEQMLGGTDDNKGMDFVNELLKVMNGFELSIEMDIKIGKDGVYYLAH
                     LKGKCHIIPSFQGDSNQCYKWVIADENNQDLQGFYKEKNLQTIDCNAIAIQAVTPPQA
                     PITYTGTKKYTAALMGLSMDFCHPGHDTIVLGGFIANPPSAGTWLYPHGFVSAAGVTG
                     MEEFFEDIHARKKLADDGEAQKAQTDLQAQAEKLKAQMEALKDQIGNSQSGANAGANY
                     EKMMELKNKMISQLSTPVTAKLLYEDFPLPVKNNEPVLVDNRFDAKQINPQESQAIVY
                     GYYTIHIENDGNGKPKPKTAGSPAGKKN"
     gene            4325..>5242
                     /locus_tag="VKU83_00090"
     CDS             4325..>5242
                     /locus_tag="VKU83_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91972.1"
                     /translation="MKKRISPPMRITLLPALLLLASILLASMQARAQKAYPVELLGLL
                     GKITVPTDAAACFAGCTKTTDPANGAVSIVNTDPTFKELNDELNKISKAAMDAANAQT
                     SAQAPNADQVQQMQQQAMQQAAAMQGKSPQEIAAAQQARAQSGAPSASELAVLKKMGQ
                     AQTAAAQINQLINEMSQKFTKLDKSEIDNVPRGANCPEVQQGGYAGPTCDCLTKRDQA
                     YQSARVAKCNAYLQQVSAIINEYMGKIKVQAAIVDDFEHTAKYGDALSNATYRQLVGS
                     VQQQALGGVVATLAAASSNWEDAAKIYAYL"
BASE COUNT         1347 a         1589 c         1387 g          919 t
ORIGIN      
        1 cccggccgcg gcaccaccgt caccgtgacg ctgaaactgg cgtgatgggg gaaccggggg
       61 cggcccgaca aaatcctata aatatccgat tgaaatatcc aaaaaatctg gggacttttg
      121 acggaccccg ccaaacccgc cgccacaaga aatgatcaag attgccatcg tcgaggacaa
      181 taacaccctg cgcagcagcc tggaaagcct gttcaaccgg acggacggga tgcgctgcgt
      241 ggcctcgttg aacaatctgc tcaatgtggt cggcgagatc ggctcggctt tgcccgatat
      301 catcctcatg gatatcggac tcccgaatat ctccgggata gaaggcgtcc ggacggtgaa
      361 aacccacttc cccgccatcc agatcctcat gttcacggtc ttcgacgacg acgaaaaggt
      421 cttcgatgcg atccgcgccg gggcatccgg ttacctgctg aaaaagaccc ctgccgccga
      481 gatcatcgac gccattcgcg agctgtacaa cggaggcgcg ccgatgacct ccagcatcgc
      541 ccggcgtgtc atccagtcct ttcaggcggc cccttccagc gtcatcaaag actaccagct
      601 caccgtccgg gagaatgaga tcctctattc gctggtggac ggcctcagct ataaaaaaat
      661 agcggataaa tattgcgtca gcatcagcac catccgaacg catatctgca acatctacca
      721 caagctccac gtcaattcga aagcagaggc ggtcgcccgc gtcctgggcc cgcacaaata
      781 agctacgcag attgaattga atcaacttgg gcgtcgtccg tccgtcgccc cggattaaaa
      841 tcatatgttc atccgattga cttccgcggg ttttaatcgt ctatttgtcg aaacagactg
      901 accatgaaaa atttttgtac agcggccacc gcggctctgc tgctcgcggc aattgtcgcc
      961 ccaacccccc gggcatgcgc tcaggtcacc aaccccggca ccaccgccgg acaaagcgcc
     1021 accgaccacg ttaacaataa cgtttcgaat ggcatcaaca acggtctcga caagaccgaa
     1081 aactccctca aaggactttt caagaaaaag aacaaggctc cgaagaacga tacggcaaag
     1141 gcatccgcca gctcaacaag ccattccacg gccaacgcca gcggcccgga cggccagaac
     1201 gcgccgacct cgctgaaggc ttactccaac tatgactttg ttcccggcga acaggtggtt
     1261 ttcgaagatc atttcacaga cgaccaggac ggcgaattcc ccgcgcactg gaaactcact
     1321 tcgggacagg gcgtcaccaa caaggtcggc gacgagcccg ccttcttcct caccgaaggc
     1381 aactatgtcc gcgtggcgcc acgcatgaag gcggaaaaaa attacctgcc cgacaacttc
     1441 acggtggagt tcgacttcta ccctacggaa ggctcgtatt atcccatgct gctgttcacg
     1501 accgccaccg acgacagccg gttcatccaa ttcggcgacg aggtcgccac cagctacttt
     1561 cccagcgatt tcagcgccca gtatccggga gacagggaca acttcaatgg caagtggcat
     1621 catgccgcca tgatcaagaa gggcaaccag atcaaatgct acgaagacca gtaccgtgta
     1681 ctggtcatcc cggattgcgg cggctgcaaa atgagcttcg tcgaggtagg gggaatcggc
     1741 gacaaggacc atccgatcgt gtttgacaat ttccgtctcg ccgaaggcgg caatatgaac
     1801 ctcatcgggc agaaattcac ggacgcgaag ttggtcacgc acgggatcaa tttcgacgtc
     1861 gataagtcat ccatccggcc ggaaagcatg ggcaccctca atatggttgc gggcatcctc
     1921 aaaagcaacc cggatctcaa gttcgagatc gacggccaca ccgacaacag cggcgacgcc
     1981 gcacacaacc tgacgctttc cctgcaacgc gctgccgcgg tcaaggcaca gctcgtcgcg
     2041 atgggcatcg acggttcgcg gttgacggca aaaggctttg gcgacaccaa accgatttcg
     2101 gataacagca cgctggaagg caaggccaac aaccggcgcg tggaattcgt caaaatgtaa
     2161 gccgccccta accctaaccg cagcaccaat gagaaagtta tttctatcag cgctcctgtg
     2221 ctgttcgggc ctcttgcccg gactcagctt gagggcgcag gcgccggcgc cggccacagg
     2281 cggcgtcggc ctttgggacg cccaccactt cctttggttc acgggccatt ggaacaaatc
     2341 cggaagcagg ttccgtttta tcgtcgatcg gggcaacacg gcaacaaccg gggcaggagg
     2401 cggcggcgcc gcgcctggca ccaccggtga taacgacgat ccgcccggta atccgcccga
     2461 cggcggtacg atcaccatcc ataccgacgg aacggtcaac ctcgacaaca gcggcgtaac
     2521 ggaatggaac aagaaaatgg acgaggccct gaagtccgag gaagactatc ttcgcgccct
     2581 tcaggacccg ggtgacaatc tcgaccccac cacccatcat ctccaccagc tattggtgcc
     2641 ggacgcgcgg cagcgggtca acgatatcca gacctataag atcggggccg accaggatat
     2701 gctgggcgcc gacagcaagt ccgcaccgcc acaacggacc ttgtcggatg ccgccgaaga
     2761 cctttgccgg gatatcaagc ccgactacga cctgatcatg aactactata agacacaggt
     2821 caagggtcac aacaatgacc tacggttccc cgcgccgcca acggccgatt acgattgtta
     2881 cagctgcgat tccagcgtgc gcaagctgta tgacacggcc atttaccatt atgcggataa
     2941 tttcgggaaa gaagaggacg atatcgtgat gaaagggctc gccatacttc acaaattggg
     3001 gctgatgggc gtaagcaccg aatcgggata ttctccggag ctcgacaacg ccttccgcaa
     3061 gaataagcag gatccctcaa aagccggcgc ctgctcgttc atcgaactta cctacttttc
     3121 ggacgccgtc tatgcgattg cgcatcacct ctatctgcgc gcggaaaaga tggtctatgc
     3181 caacaagaac aattacaaag cggcggaggc gatcatccgg gcctacctta agcaagcgcg
     3241 ggcctgggcc atgcgctccg gtaacacgga gggcaatgac atgctgcccc tgctcgctag
     3301 cctcgcgggc caggccgtcg acttttatat caaggcattc cgccacgacg actggcggca
     3361 agtcggtaat gccacgctta tcctgcaaat gatccgcgac gagcaaatgt tagggggaac
     3421 cgatgacaac aagggcatgg atttcgtcaa cgagttattg aaggtcatga acggcttcga
     3481 gctgagcatc gagatggaca tcaagatcgg gaaggatggg gtctattacc tggcgcacct
     3541 caaaggcaaa tgtcatatca ttccgtcctt ccagggagac tccaatcaat gttataaatg
     3601 ggtaatcgcc gatgagaaca accaggacct ccagggattt tacaaggaaa agaacctcca
     3661 gaccatcgac tgcaatgcca tcgccatcca ggcggtcacg ccgccgcaag cgccgatcac
     3721 ttacaccgga acaaaaaaat acaccgcggc gctcatgggg ctcagcatgg atttctgcca
     3781 tccggggcat gatacgatcg ttctgggagg ttttatagcc aacccgccgt cggcgggcac
     3841 gtggctctac ccgcacggtt tcgtcagcgc agcgggcgtc acgggcatgg aagaattttt
     3901 cgaagacatc cacgccagga agaaactggc ggacgacggc gaagcccaaa aagcgcagac
     3961 cgatcttcag gcccaggcgg aaaagctaaa ggcgcagatg gaggcgctca aggaccagat
     4021 aggcaattcc cagtccggcg caaatgcagg cgccaactat gaaaagatga tggaattgaa
     4081 gaataagatg atctctcagc tcagcacacc ggttaccgca aaattgttat acgaggattt
     4141 cccgctgccc gtgaagaaca acgagcctgt gcttgtggat aaccgatttg acgccaagca
     4201 gatcaacccg caggaaagcc aggctatcgt ctatggatac tacacgatcc atatcgaaaa
     4261 cgacggcaac ggcaaaccga aacccaagac cgccggttcg cctgccggca aaaaaaatta
     4321 aaccatgaaa aaaagaatct ctcctcccat gcgcattacg ctgctaccgg ccctgttgct
     4381 gctggccagc atcctgcttg cgtcgatgca agcccgggcc cagaaagcct atccggtcga
     4441 attgctgggc ttgcttggca agatcaccgt tcctacggat gccgccgcct gctttgccgg
     4501 atgcacgaaa acgaccgatc cggccaacgg cgctgtaagc atcgtcaaca ccgatccgac
     4561 cttcaaggag ctcaacgacg agctgaacaa gatcagcaag gcggccatgg acgcggcaaa
     4621 tgcacagaca tctgcccagg cacccaacgc cgaccaggtc cagcagatgc agcagcaggc
     4681 catgcaacaa gctgccgcca tgcaaggcaa gagcccgcag gaaattgctg ccgcacagca
     4741 ggcacgtgcg cagtcaggcg ctccatccgc gtcagagctg gcagtactga aaaaaatggg
     4801 ccaggcccag acggcagccg cccagatcaa tcagctcatc aatgagatgt cgcaaaaatt
     4861 taccaagctg gataaatccg aaatcgacaa cgtcccgcgt ggcgccaact gtcccgaagt
     4921 tcaacagggc ggatatgccg ggcccacgtg cgattgcctg acgaaacgcg accaggctta
     4981 tcagtcggcg cgggtagcca aatgcaatgc ttatctgcag caggtatcgg ccatcatcaa
     5041 tgaatacatg ggcaaaatca aggtacaggc ggctatcgtc gacgattttg aacataccgc
     5101 aaaatatggc gatgccctgt cgaacgccac gtaccggcag cttgtcggct ccgtgcaaca
     5161 acaggccctc ggaggggtcg ttgccaccct cgcggccgcc agcagcaact gggaagatgc
     5221 agcgaaaatc tacgcgtacc tc
//
LOCUS       DATGKE010000007         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_310717, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000007 DATGKE010000000
VERSION     DATGKE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310717"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VKU83_00095"
     CDS             complement(<1..473)
                     /locus_tag="VKU83_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91973.1"
                     /translation="MKKFECPDFVLGDKITEEQYSFFDKYGVIIFRNFLNSATVQLFI
                     HELNRIEKQWLDEGRDKVNGIPLKFGKDQNGNKMIQRMCFLSLHSEPLHEMLQDPRVK
                     ALVGLLYPYEGRISENEKDGLILNHYVRMPESSFTQMGWHTDSPRDLFLGQKIMP"
     gene            complement(817..1452)
                     /locus_tag="VKU83_00100"
     CDS             complement(817..1452)
                     /locus_tag="VKU83_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HLI91974.1"
                     /translation="MPQTTFFTVRQEWQGAWQVSAFRRRTISGLIIIIAILSSLPVFF
                     QYIERRQGAQLNDPVLGLLAPHNVSLPLFVVIWAISAYYIYRAAQTPQLFLNFLWAYI
                     VLLIFRSLTITLIPLDPPAGLIPLVDPLSNFFYGDKFVTKDLFFSGHTSTVFLLYLSL
                     PRRAGRNLALIATTVVAVLLLVQHVHYTFDVLGGLVFAWIAHKIATRVILR"
     gene            complement(1487..>2627)
                     /locus_tag="VKU83_00105"
     CDS             complement(1487..>2627)
                     /locus_tag="VKU83_00105"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLI91975.1"
                     /translation="TRFKSPWLTGELEWTPAMIRKAVVNMALKLGKPILSLTNSDYND
                     SGLSDLLVEKGDAYEINLQVFYLLRDSITGWPGGKPGNQSPTHPERSVPFPKRCLIFS
                     PHPDDDIISMGGTFMRLHDQGHDVHVAYQTSGNIAVTDEFVTRFLDFAVGFEELSGID
                     SKKSSQILEQATRYLKTKKASDVDTPEIRAIKGLIRRCEARATCRYVGIRDENIHFMN
                     LPFYETGTIEKKPMGEEDVKLTMELLRRLKPQQIYAAGDLADPHGTHKVCLDIVFESL
                     RRLKAAGDTWLNDCWLWLYKGAWQEWDISEIEMAIPMSPDQVLKKRFGIFIHQSQKDM
                     VPFQGSDSREFWLRAEDRNANTANLYAQLGLTKYAAMEAFVRWYY"
BASE COUNT          654 a          650 c          696 g          627 t
ORIGIN      
        1 atcggcatga tcttctgccc caggaaaaga tcccgggggc tgtcggtatg ccagcccatc
       61 tgagtgaacg agctttcggg catccgaacg taatgattga ggatcaggcc atccttttcg
      121 ttttcgctga tccggccctc atacggatac aacaatccca ccagggcttt aacgcgcgga
      181 tcctgcagca tttcatggag cggctcgcta tgcagggata aaaaacacat gcgctggatc
      241 atcttattcc cattctggtc cttaccgaat ttcaacggga taccattgac cttgtcgcgg
      301 ccctcatcca gccattgctt ttcaattctg ttaagctcat gtatgaataa ttgaacggtc
      361 gccgaattca gaaaattcct aaaaatgatc actccgtatt tatcgaaaaa gctatactgt
      421 tcttccgtta tcttatctcc caggacaaaa tcggggcatt cgaatttttt cataaatgct
      481 agctttattt aagaaaagga agggtccatc cctttccgct tcagagcttt aaaatattgg
      541 gttaattgag ttttaacaaa ttgtgaaatc ttcattaatc atcaaatgta agaaaattag
      601 gttttttcgt gttaatttac aaggcttttt atcgacataa gccaataaat atactacaac
      661 attgcccaac caatcgttcg gcgaagcaat atttacctat tttcttgggc ttatgggcta
      721 ttgacgatac ccattaccgg gaaacccaaa gtccggcaaa tatacgcaag atagacactt
      781 tgccaatagg ttaaaagccg cgaattccca tatatattag cgtaggatca ccctggttgc
      841 gatcttatgg gctatccagg cgaacacgag cccgcccagt acgtcaaagg tataatggac
      901 atgctgtacc aatagcagta ccgccacgac ggttgtggcg atgagcgcga ggttacgacc
      961 ggccctacgg ggtaacgaaa ggtacaaaag aaagacggtg gaagtgtgtc cggagaaaaa
     1021 gaggtctttg gtgacaaact tatccccata aaaaaaattg cttagcggat cgaccagcgg
     1081 gatcagcccg gcgggcggat cgagggggat gagggtgatc gtgagcgacc ggaagatcag
     1141 aaggacgatg taagcccaaa gaaagtttaa aaacagctgc ggggtctgcg ccgcccgata
     1201 tatataataa gccgatatcg cccagatcac cacaaacaga ggaagagaga cattatgcgg
     1261 cgccaatagt ccgaggaccg ggtcattcag ctgcgcgccc tgccggcgtt cgatgtattg
     1321 aaagaacaca ggaaggctgg acaggatggc aataataatg ataaggccag agatcgtccg
     1381 gcgacgaaag gcggacacct gccatgcccc ctgccattcc tgccggacag tgaaaaatgt
     1441 tgtttgtggc attgctaatc cacaaaaata cggaattagc gcaaaattaa tagtaccatc
     1501 ttacaaatgc ttccatggcc gcatatttgg tcagcccgag ctgcgcatag aggttagcgg
     1561 tattggcatt gcggtcttcg gcccgcagcc agaactcacg gctgtcgctg ccctggaaag
     1621 gcaccatatc cttttgcgac tggtggataa agataccgaa gcgcttcttg aggacctgat
     1681 cggggctcat ggggatagcc atttcgatct cgctgatgtc ccattcctgc caggcgcctt
     1741 tgtacagcca gagccagcag tcgttgagcc aggtatcgcc ggcggccttg aggcggcgca
     1801 gcgattcgaa cacgatatcc agacatacct tatgggtgcc atgcgggtcg gccaggtcgc
     1861 ctgcggcata gatctgctgg ggtttgagcc ggcgaaggag ctccattgtc agctttacgt
     1921 cttcctcgcc catcggtttt ttttctatcg taccggtctc atagaaaggc aggttcatga
     1981 aatggatgtt ctcatccctg atccccacat aacggcaggt agccctggcc tcgcaacggc
     2041 ggatgagtcc cttgatagcg cggatctcgg gcgtatcgac gtcactggcc ttctttgtct
     2101 tgagataacg tgtggcctgt tccaggatct gggaggattt tttgctatcg atccccgaca
     2161 gttcttcgaa gcctacggcg aaatccagga agcgggtgac aaattcgtcg gtaacggcga
     2221 tattcccgga agtctgatag gcgacgtgta catcgtgtcc ctgatcatgg agccgcatga
     2281 aggtcccgcc catcgagatg atatcgtcgt cggggtgggg ggagaagata aggcaacgtt
     2341 tggggaaggg caccgaccgc tcgggatgcg tgggagattg attgccgggc ttgccgccgg
     2401 gccagccggt gatgctgtcc cgaagcagat agaatacctg caggttgatc tcgtaagcat
     2461 cgcctttttc caccagcaga tcgctcaggc cgctgtcgtt gtagtcgctg ttggtaaggc
     2521 tgaggatggg ctttcccagc ttcagggcca tattgaccac tgccttccgg atcatggccg
     2581 gagtccactc gagctcgccg gtgagccagg gcgatttgaa acgggtc
//
LOCUS       DATGKE010000008         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_204510, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000008 DATGKE010000000
VERSION     DATGKE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204510"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VKU83_00110"
     CDS             <1..304
                     /locus_tag="VKU83_00110"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLI91976.1"
                     /translation="GTSTYYRYLGFRPLSEAILGRMLTLGLKEFGNVGGFNFSLSGPT
                     EYPNALVEVAFLSNREDEKRIMRPAFRKAVAQKIYLGIVDWLKAMEPDRPVHANKK"
     gene            308..709
                     /locus_tag="VKU83_00115"
     CDS             308..709
                     /locus_tag="VKU83_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HLI91977.1"
                     /translation="MKGYLMQEKDWVPAEDLFIESFSPENAYGVVFEDDGDTAYFYAV
                     EKDAEGAGMRVLDALHIWDKDCGTAEPGLLQIVWSGDWLKAALVIGGRCHALFDFEAH
                     GGYNVNEFPPPNGIWTRGERKLTAEVVERIF"
     gene            complement(769..1491)
                     /gene="pgl"
                     /locus_tag="VKU83_00120"
     CDS             complement(769..1491)
                     /gene="pgl"
                     /locus_tag="VKU83_00120"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HLI91978.1"
                     /translation="MPLHVFKDPDELSHAVAAWIADTIAETLQKSERFTIALSGGSTP
                     KALHKLLASAPYKDRIEWSRLHVFWGDERAVPFDDDRNNAKMAYDTLLDLVPVPPAQI
                     HVMRTDIPPPESAAEYEKILHTYFGPAGAGFDLVLLGMGDDGHTLSLFPGTAAVHEEK
                     AWTIAFYLEAQRMYRITLTKTIVNRSQRVAFLTTGANKAHALKEVQQGKYNPDLYPAQ
                     EIRPANGELHWFVDAAAAAELG"
     gene            complement(1501..>2451)
                     /gene="zwf"
                     /locus_tag="VKU83_00125"
     CDS             complement(1501..>2451)
                     /gene="zwf"
                     /locus_tag="VKU83_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLI91979.1"
                     /translation="YRIDHYLGKESVQNILSLRFANALFEPIWNRHYIEHVQITAAET
                     VGVEGRGGYYENSGALRDMVQNHILQLMCMIAMEAPVSFDANEIRNKKVDVLNAIRKI
                     SKEDVNQYAVRGQYSEGWIKGEKVPGYKSEKSVNPNSHVETFAAVKFYIDNWRWQDVP
                     FYVRTGKRMNQKTTNITIQFKKAPSYAFPPEAAETWRANRLTISIQPEMEVRLRFQAK
                     RLGQSMSLNPVDMVFNPEDDTHEPEAYETLLLDVMEGNPTLFMRADQVEAAWRIVMPI
                     LETWESRPPVDFPNYSPDSWGPEDAEALIARDGHNWITIY"
BASE COUNT          490 a          674 c          736 g          551 t
ORIGIN      
        1 gggcacgagt acctattacc ggtatctggg tttccgcccg ctcagtgagg ccatcctggg
       61 cagaatgctg accttgggtt tgaaggagtt cgggaacgtg ggcggtttca atttttccct
      121 cagcgggccg acggaatacc cgaatgcgtt ggttgaggtg gcctttctaa gcaatcgcga
      181 ggacgaaaag cggatcatgc ggccggcctt cagaaaggca gtcgcgcaaa agatctattt
      241 agggatcgtg gactggctga aagcgatgga gccggatcgc cctgttcacg cgaacaaaaa
      301 ataaaccatg aagggatacc tgatgcagga aaaggactgg gttcccgccg aagacctttt
      361 catcgaaagc ttttcgccgg aaaatgccta cggcgtagtg ttcgaagacg acggagatac
      421 cgcttatttc tatgccgtgg aaaaggatgc cgaaggcgcc gggatgcggg tgctggatgc
      481 cctgcatatc tgggacaaag actgcgggac cgcggagcct ggcctcctgc aaatcgtctg
      541 gtcgggagac tggctgaagg cagcgctcgt catcggcggc cgctgccatg ccttattcga
      601 ttttgaggcc cacggcggct ataatgtcaa cgaatttccg ccgccgaacg gcatctggac
      661 gcgcggggaa aggaagctga cggcagaggt cgtggaaagg atcttttgat cgcgaccact
      721 ccgacggttg atcgccgcca cccgggcttg atcgccgcca ccctacgctt agcccagttc
      781 cgccgcagcc gcggcgtcca cgaaccaatg caattcgcca ttcgccggcc tgatctcctg
      841 ggcgggataa aggtcggggt tgtattttcc ctgctgcact tcctttaacg cgtgcgcctt
      901 gttcgcgccg gtggtcagaa aggcgacccg ctgcgaccgg ttgacgatgg tcttggtcag
      961 cgtgatgcgg tacatgcgct gtgcttccag atagaatgcg atcgtccacg ccttctcttc
     1021 gtggacggcg gcggtgccgg gaaaaagcga caaggtatgc ccgtcgtcgc ccatgccgag
     1081 gaggaccagg tcaaaaccgg ctcccgccgg accgaaatag gtgtgcagga tcttctcgta
     1141 ttccgcggcg gattccggtg gaggaatatc ggtgcgcata acatgaatct gcgcaggagg
     1201 caccggcacc aggtcaagga gcgtgtcata cgccatcttc gcgttgtttc ggtcgtcatc
     1261 gaacggaacg gcgcgctcat cgccccaaaa gacatgcagc ctggaccatt ctatccggtc
     1321 cttgtatgga gcggacgcca gcagtttgtg aagcgctttt ggcgtgcttc cccccgaaag
     1381 ggcaatcgtg aaacgttcgc ttttttgaag ggtttcggcg atggtatcag cgatccaggc
     1441 ggctaccgca tggctgagtt catccgggtc cttaaaaacg tgtaatggca taacaggcgg
     1501 ttagtagatc gtgatccaat tgtgaccatc gcgggcgatg agggcttcgg catcctcggg
     1561 tccccaggag tccggcgaat agttggggaa atcgacgggc ggccggcttt cccaggtttc
     1621 gaggattggc atgacgatcc gccaggctgc ctctacctgg tccgcccgca tgaacagggt
     1681 cgggtttccc tccataacat ccagcaacag ggtttcataa gcttccggtt catgggtgtc
     1741 atcctccgga ttgaacacca tatcgacggg attgagcgac atcgactgcc cgagtcgctt
     1801 cgcctggaaa cgcaacctga cttccatttc aggctgaatg ctgatcgtca ggcgattggc
     1861 ccgccaggtt tccgcggctt ccggcgggaa ggcgtagctc ggcgcctttt tgaattggat
     1921 cgtgatattg gtggttttct ggttcattcg cttgcccgtc cggacataga aaggaacgtc
     1981 ctgccagcgc cagttatcga tataaaactt tactgccgca aacgtctcca cgtgagagtt
     2041 ggggttgacg ctcttttcgc ttttataacc cgggaccttt tcgcccttta tccatccttc
     2101 ggagtattgc ccgcgcaccg catactgatt gacgtcttcc ttgctgatct tgcgaatggc
     2161 gttcaggacg tccaccttct tgttacggat ctcgttggca tcaaaagaga ccggcgcctc
     2221 catggcaatc atgcacatga gctggaggat gtggttctgg accatgtcgc gcagtgcgcc
     2281 ggagttttcg tagtagccgc cgcgcccttc gacgccgacc gtttccgcgg ccgtgatctg
     2341 cacgtgctcg atataatgcc ggttccagat cggttcgaaa agcgcgttcg cgaaacgcag
     2401 cgagaggata ttctggaccg actccttgcc gagataatgg tcgatgcggt a
//
LOCUS       DATGKE010000009         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1032960, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000009 DATGKE010000000
VERSION     DATGKE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032960"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="VKU83_00130"
     CDS             <1..2477
                     /locus_tag="VKU83_00130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HLI91980.1"
                     /translation="MPVGGICAGCVYAGGDGRLWLWDIFNQNQVGVVTKRLPIPLEGF
                     NAKEIDNVEGALYLEPLTETSPLQQGYALLITGNGTTSLRRLHQDDWDEITFETTYPV
                     ATVRYSDRHLPVEVTAELFSPYIPSNARESGMPATIQALTVRNTASTGSVSVQLVGWL
                     ENKMLWITEKTNAGFSRTNEIVQRGPFKAISLGCALADEDLGRAADFGNMCLAAMDED
                     AVLIPDISKATEGPALAIRDLVKTSNGSPMAAVSVNHTLDAGKEFKSDFIISWYTPNI
                     SFNNRGDEVTAHDAGKHYYATVFDSAAAVAGYISQNYEALRDHSFLWKETWYDSSLPW
                     WFLERTFNNISAIATTSTHRFGDGRYYAWEGVGCCHGNCTHVYQYAHAVSRIFPELER
                     DTRERVDLGPGVGYDEATGMIRIRGEKTGPSIDGQAGTVLRIYREHQMAADDQFLRRN
                     WAKIRKAVMFIMEQDKNHDGMEDTPMENTLDALWHGEISWIVGLCIAGVRAGQAMAEE
                     MNDSDFAARCRQYVELGRKNMEAELFNGEFFIHRPDPTVGKKEIGSFNTCHIDQVYGQ
                     SWAWQVNLGRILGKEKTMSALHALWKYNYMPDVGPYLKVHPGGRFYALAGEGGMIMNT
                     NPANDANPYGNAKAWQVGYFSECMSGFEHQVASHMMAEGMTQESLVLTRSIHDRYHAY
                     RRNPFNEIECSDHYGRAMASYGTFISACGFTYHGPKGEIGFAPRISPANFKAPFTAAE
                     GWGTYSQQRHPKSFSAQLRLKYGRLTINKFAVEMDNAMAAARAVVKLDDTAVPCRFTQ
                     TGNRCEIALHQPAVLSANSVLALAIN"
     gene            2484..3596
                     /locus_tag="VKU83_00135"
     CDS             2484..3596
                     /locus_tag="VKU83_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLI91981.1"
                     /translation="MKKRKLIVVGGFLGSGKTTAIHQAARCLRKDHKKAGMITNDHGT
                     LHVDTAFMKGQGIPTAEVSAGCFCCHYAELADRIEWLLRTTGPDIIFAEPVGSCVDLA
                     ATVINPLLTANAGRYDVVLSVFADSRLLLAFLTGNKPVFSDNVNYIYENQLREADILV
                     VNKIDLLAADQLEWAKERMGREYGDKIVLFQNSLAEESVGQWLACCDNYSDRELRPAL
                     DIDYGIYGAGEAQLAWMDEEIGIVSPDANAVRTAYELMQMLHRRITGNGWPIGHLKFL
                     LDDGQEQKKVSFTTIPGDGPAEPADAADAAHAQADRAVVIVNARIHAAPELVRQLTRR
                     TILDLEAATGCRITEHRASAFRPGYPRPTHRIIKRV"
     gene            complement(3593..>4601)
                     /locus_tag="VKU83_00140"
     CDS             complement(3593..>4601)
                     /locus_tag="VKU83_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLI91982.1"
                     /translation="QKPTGGKVEVFCLEPPAESGGMAAAMARTGAVRWKCLIGGASKW
                     KPGQVLAKQVGEVQLEARYLENCGDAFLIELSWRPAALSFAELLHRAGLIPLPPYLHR
                     APEDSDYERYQTVYARHEGSVAAPTAGLHFTQGVFDSLAASGIRSVFVTLHVGAGTFL
                     PVKSATLEQHAMHTEFIDVACETIGALRGTAMSGDPVIPVGTTSARTIESLYWLGLKA
                     HRNPDIGLEDFVVHQWEAYEAGAEPPAEIALGALLEWLDRRKAAQLRTTTQLLIAPGY
                     RWRVATGLITNFHQPESTLLLLVASLIGGDWRKVYDYALAHGFRFLSYGDGCLFLPGK
                     A"
BASE COUNT         1010 a         1331 c         1374 g          886 t
ORIGIN      
        1 gaatgccggt gggcggcatc tgcgccggct gtgtatacgc gggcggcgat ggtcggttat
       61 ggctctggga catcttcaac cagaaccagg tgggggtggt caccaaacgg ctgcctattc
      121 cgttggaagg ttttaatgcc aaagagatcg ataacgtcga aggcgccttg tacctggaac
      181 cgctcacgga gacaagcccg ttgcagcagg gctacgcgct tttgattacc ggcaacggga
      241 cgaccagcct ccggcgcctt caccaggacg actgggacga gatcaccttc gaaacgacgt
      301 acccggttgc caccgttcgt tattcggaca ggcacttgcc ggtcgaagtg actgccgaac
      361 tcttctcgcc gtatattccg tcgaatgccc gggagagcgg gatgccggcg accattcagg
      421 cccttacggt aagaaatacc gcctccaccg gttccgtgtc ggtccagctg gtcggctggc
      481 tggaaaataa aatgctctgg ataacggaga agaccaacgc cgggtttagc cgcacgaacg
      541 agatcgtgca acggggcccg ttcaaggcga tcagcctggg ctgcgcgctg gcagacgaag
      601 accttgggcg ggccgctgat ttcggcaata tgtgcctggc tgctatggac gaggatgccg
      661 tcctcatccc cgacatttcc aaagcgacgg aaggtccggc gctggcgatc cgggacctgg
      721 tcaaaacatc caacggttcg cccatggccg ccgtctccgt gaaccacacc ctcgatgccg
      781 gaaaggaatt caagtcggat ttcattatct cctggtacac gcccaatatt tcatttaata
      841 acaggggtga tgaagtgacc gcacatgacg ccgggaagca ttactatgct acggtgtttg
      901 acagcgccgc ggcagtggcc ggctacatct cgcagaacta tgaagcactc agggatcaca
      961 gctttctctg gaaagagacc tggtatgaca gcagcctgcc gtggtggttc ctggagcgta
     1021 ctttcaataa catttcggcc atcgcgacca cctcgacgca tcgcttcggg gacggcaggt
     1081 attatgcatg ggaaggcgtg ggctgttgcc atggaaactg tacgcacgtc tatcaatacg
     1141 cccatgccgt ctcgaggatc tttcccgaac tggaaaggga tacccgcgaa agggtcgacc
     1201 tgggcccggg cgtcggatat gacgaagcca ccggcatgat ccggatcagg ggcgaaaaaa
     1261 ccggtccgtc gatcgacggt caggccggca ccgttctgcg catttaccgg gagcatcaga
     1321 tggccgcgga tgaccagttc cttcgaagga actgggcgaa gatcaggaag gcggtgatgt
     1381 ttatcatgga acaggataag aaccatgacg gcatggaaga tacgccgatg gaaaatacgc
     1441 tggatgcttt atggcatgga gagatctcgt ggatcgtcgg actttgtatc gccggagtcc
     1501 gcgccggaca ggcaatggcc gaggagatga atgacagtga ctttgccgcg cggtgcaggc
     1561 agtatgtcga acttggccgg aagaatatgg aggcggagct gttcaacggg gaatttttca
     1621 ttcaccggcc agatcccacg gtcggtaaaa aggagatcgg gtcgttcaac acctgtcata
     1681 tcgaccaggt gtatggccaa tcctgggcat ggcaggtcaa cctcggccgg atcctcggca
     1741 aagagaagac catgagcgcc ctgcacgcgt tgtggaagta taattatatg ccggacgtcg
     1801 gcccttatct caaggttcac ccgggcggtc ggttctacgc cctcgcaggg gaaggcggca
     1861 tgatcatgaa taccaacccg gcgaatgatg cgaaccctta tggcaatgcc aaggcatggc
     1921 aggtgggata tttcagcgag tgcatgagcg gcttcgaaca ccaggtagca tcgcacatga
     1981 tggcggaggg catgacgcag gagtcgctgg tcctcacgcg gtcgatccac gatcgttacc
     2041 atgcttaccg acgcaacccg ttcaacgaga tcgagtgcag cgatcattat ggccgagcca
     2101 tggcaagcta tggaaccttt atcagcgcct gcggttttac ctatcatggc ccgaagggag
     2161 agatcggctt tgctccccgc atctcgccgg cgaacttcaa agcgccgttc accgcagcgg
     2221 aagggtgggg gacttacagc cagcaacggc atccgaaatc gttttcagcg caacttcggc
     2281 tgaaatacgg cagactgacc atcaacaaat tcgccgtgga aatggataat gccatggcag
     2341 ccgcccgcgc cgtggtgaag cttgacgata cggccgttcc ctgccgcttt acgcagaccg
     2401 gcaaccgctg cgagatcgcg ctgcatcagc ctgcggtact atccgccaat tcggtcctgg
     2461 cattggccat caactaaagc aagatgaaga aacggaagct catcgtggtc ggcgggttcc
     2521 tcgggagcgg aaagacgacc gccatccatc aggccgcccg gtgtctccgc aaggaccaca
     2581 aaaaggcagg catgatcacc aatgaccatg gaacgcttca cgtggatacg gcgttcatga
     2641 aaggacaagg gattccgacc gcggaagttt cggcgggctg cttttgctgc cattacgctg
     2701 aattggcgga ccggatcgaa tggttgctcc gaaccacggg accggatatc atttttgctg
     2761 aacccgtcgg ttcctgcgtc gacctcgccg cgaccgtcat caatccgctg ctgaccgcaa
     2821 acgccggccg ttatgacgtc gtcctgtcgg tattcgccga cagcaggctt ttgctggcat
     2881 ttttaacggg gaacaagcct gttttcagtg acaacgtcaa ttatatttat gagaaccagc
     2941 taagagaagc cgacatcctc gttgtgaata agattgatct cctggcggcg gaccagttgg
     3001 aatgggcaaa agagcggatg ggcagggaat atggcgataa gattgtttta tttcagaatt
     3061 ctctcgccga agaaagcgtc ggccaatggc tggcctgctg tgacaactat agcgatcggg
     3121 agctgcgccc ggcgctggat atagactatg ggatctatgg ggcgggcgaa gcccaattgg
     3181 cctggatgga tgaagaaatc gggatcgttt cgccggatgc gaatgcagta agaaccgctt
     3241 atgagctgat gcagatgctg catcgccgga taaccggcaa tggctggccc atcgggcacc
     3301 ttaagttcct gctcgatgac ggccaggaac agaaaaaggt cagcttcacg accattcccg
     3361 gtgatgggcc cgctgaaccg gccgacgcgg cggacgcggc gcacgcgcaa gccgacaggg
     3421 cggtcgttat tgtcaatgca aggatacatg cggcgccgga actcgttcgg caactgacgc
     3481 gccggacgat tctggatctc gaggcggcaa ccggctgccg gatcacagag catcgcgcct
     3541 cagcgtttcg acccggctat cccaggccca cccacagaat aatcaagcgg gtctaagcct
     3601 tgccgggcag gaacaggcaa ccgtccccgt agctcaggaa gcggaatccg tgggccagcg
     3661 cataatcgta gaccttgcgc cagtctccac cgatgagaga tgccacaagc aataagagcg
     3721 tgctttccgg ctgatgaaag ttggtgatca gcccggtcgc cacgcgccag cgatagccgg
     3781 gcgcaatgag caactgcgtg gttgttctca gctgtgccgc cttccggcga tccagccatt
     3841 cgagcagtgc gcccaacgcg atttctgccg gcggttccgc cccggcctcg taggcctccc
     3901 actggtgcac cacaaagtcc tccaacccga tgtccgggtt gcgatgcgct tttaagccca
     3961 gccagtagag gctttcaatg gtccgggcgg acgtagtccc taccgggatc accgggtcgc
     4021 cggacatcgc cgtcccgcgc aacgcgccga tggtctcgca tgccacatcg ataaactctg
     4081 tatgcatcgc gtgctgttcc agcgtcgccg acttcaccgg cagaaatgtc cccgcaccca
     4141 catgcagcgt gacaaagacc gatcggatgc cgctcgccgc gagactgtcg aaaacgcctt
     4201 gcgtaaaatg caggcccgcc gtcggcgccg cgaccgatcc ctcatgacgg gcgtagactg
     4261 tctggtaccg ctcgtaatcc gaatcctccg gcgcgcgatg aagataaggt ggcagcggga
     4321 tcaatccggc ccggtgcagc agttcggcaa aagagagcgc cgcaggtcgc caggacagtt
     4381 cgatcaggaa cgcgtcgccg cagttttcga ggtagcgcgc ttccagttgc acttcaccga
     4441 cctgcttcgc cagcacctgg cccggcttcc attttgacgc tccgccgatc aggcatttcc
     4501 aacgcaccgc cccggtgcgg gccatcgccg ccgccattcc gccggattcc gccggcggct
     4561 ccaggcaaaa gacctctact ttgccacccg tcggcttctg a
//
LOCUS       DATGKE010000010         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_100977, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000010 DATGKE010000000
VERSION     DATGKE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100977"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VKU83_00145"
     CDS             complement(<1..887)
                     /locus_tag="VKU83_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HLI91983.1"
                     /translation="MTVPTALTPRQQGYFFPAEFSRHRATWLSWPHKEASWPGKIQAI
                     YPAYSRFVRELTRGEDVCINVKDAAMESAAIAHLESAAVDLARVQFFHFPTNDAWCRD
                     HGPAFLVNPARQDGYKKVIVDWNYNAWGNKYPPFDLDDDIPTHIGKALGLPVYYPGII
                     MEGGSVDFNGAGTLITSTCCLLNPNRNPALDRDAIEDYLREFYGVEQVLWVSEGIVGD
                     DTDGHIDDTVRFVNQDTVVAVVEHDRQDENYQRLKGNLVELSRMRLLNGKQLNIVELP
                     MPSPVVYEDQRLPASYANF"
     gene            complement(897..1772)
                     /locus_tag="VKU83_00150"
     CDS             complement(897..1772)
                     /locus_tag="VKU83_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HLI91984.1"
                     /translation="MSKIKVGLVQMSCSADKASNLQKAMDRIREAAAGGAQIVCLQEL
                     FSSLYFCDVEEYDHFKLAEPVPGPATEALGQLAAQLGLVIVASLFEKRAKGVYHNTAA
                     VLDADGAYLGKYRKMHIPDDPAYYEKFYFTPGDLGYKVFSTKYGKIGVLICWDQWYPE
                     AARITALMGAEILFYPTAIGWAVAQDQATNTEQYNAWQTIQRSHAVANGVHVISVNRV
                     GLEQNGQMNFWGGSFVANPFGSVLFQASHDREETKVVEIDTEATDRYRTHWPFMRDRR
                     IDSYQPITRRYIDED"
     gene            complement(1881..2951)
                     /locus_tag="VKU83_00155"
     CDS             complement(1881..2951)
                     /locus_tag="VKU83_00155"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HLI91985.1"
                     /translation="MSLPKFSSGPQSFHTELKKRISNYFEEVGKAKTGDYHLFLKAVI
                     LMVGFVFIYIHLVFFTPAAFWAILESVLLGGLVAAIGFNVMHDGAHGSFSKYKWVNSL
                     AAFSLNILGGNSFMWNMKHNVVHHSYTNVEGVDDDIDIQPWMRMSEAQKRYRLHRYQH
                     LYFWVLYSVLYIFWIFVLDYQKYFSNKIGTMPLKKMSLGDHFVFWGFKVFHAFLFIFL
                     PIYRLGWYDWLVSFSIFTMVAGFVLSMVFQLAHTVQATAFPKADEATGKMEDEWAIHQ
                     LRTTANFATRNPVVSWLVGGLNFQIEHHLFPKISHVHYPAISKIIRQACQEYGLVYIE
                     FPRVRYAVASHVAFLRQMGRKK"
     gene            complement(3072..3809)
                     /gene="bshB1"
                     /locus_tag="VKU83_00160"
     CDS             complement(3072..3809)
                     /gene="bshB1"
                     /locus_tag="VKU83_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HLI91986.1"
                     /translation="MKLDILAIAVHPDDVELGCSGTLMMERANGKRTGVVDLTRGELG
                     TRGTPELRALEAAKAAGILGLDARENLGMKDGFFRNDEEHQRLLIRAIRKYRPEIVLG
                     NALEDRHPDHGRAGQLIREACFLSGLRKVETLDEGGSPQEHWRPKYLFHFIQDRYTHP
                     SFVYDISPVFEAKLQSIRAYSSQFYSTGYEADEPQTYISSPEFLNAIIGRHQLLGKMI
                     GVPYAEGFLTEKVVGVRNFDAFVQADT"
BASE COUNT          959 a         1157 c         1005 g          936 t
ORIGIN      
        1 tagaaattcg catagcttgc cggcagccgc tgatcctcat agaccaccgg cgacggcatc
       61 ggcagttcga cgatattcaa ctgcttcccg ttcagcaggc gcatccgcga cagctcaacc
      121 aaattgcctt tcaagcgctg ataattttca tcctgccggt catgctccac aaccgccacc
      181 accgtgtcct ggttcacaaa acgcaccgta tcgtcgatat gcccgtcggt atcgtcacca
      241 acaattcctt cgcttaccca caagacctgc tccactccgt aaaattcacg caaatagtct
      301 tcgatcgcgt cccggtcgag agcagggttc cggttaggat tcagtaaaca gcacgtcgaa
      361 gtgatcagcg ttcccgcgcc gttaaaatct acagatccgc cttccatgat gatgcccgga
      421 taatagaccg gcaagccaag ggcttttcca atatgcgtcg gaatatcgtc atccaggtcg
      481 aacggcgggt acttattgcc ccaggcatta taattccagt ccacaatgac ctttttatag
      541 ccatcctgcc ttgccgggtt gacaaggaaa gccggaccat gatcccggca ccaggcatca
      601 ttcgtaggaa agtggaagaa ctgtaccctg gcgagatcga ccgccgctga ttccaaatgt
      661 gcaatcgcgg cagattccat tgccgcgtct ttcacgttga tgcaaacgtc ttcgcccctg
      721 gtcagctccc gcacaaaacg gctgtaggcc ggatagatgg cttgtatctt gcctggccac
      781 gaggcctcct tatgcggcca gcttagccag gtagcgcggt gccgcgagaa ttcggcgggg
      841 aaaaaatacc cttgctgccg gggcgtaagg gctgtaggta cagtcatacc ggataattag
      901 tcctcatcaa tatatcgccg cgtaatcggt tggtacgaat cgatcctgcg gtcgcgcata
      961 aacggccaat gcgtgcggta cctgtcggtc gcctcggtat cgatttcaac tactttcgtc
     1021 tcctcccggt catgtgatgc ctggaacagc accgaaccaa agggattggc gacaaacgat
     1081 ccgccccaaa aattcatctg accattctgc tcgaggccga cccggttgac acttatcacg
     1141 tggacgccgt tagccaccgc atggctacgt tggatcgttt gccaggcatt gtactgctct
     1201 gtattggtgg cctggtcctg ggcaaccgcc cagccgatcg ccgtggggta gaacaatatt
     1261 tccgctccca tgagggcggt tatgcgcgcc gcctcgggat accattgatc ccagcagatc
     1321 agaacgccga tctttccata tttcgtcgaa aaaactttgt aaccgaggtc tcccggggtg
     1381 aaatagaact tctcgtagta cgcggggtcg tcggggatgt gcatcttccg gtatttgccc
     1441 aggtatgcgc catcggcatc cagcaccgca gccgtgttat gatagacccc cttggcccgt
     1501 ttctcgaaca ggctggccac gatcaccagc cctagttgcg cggctaattg tcccaacgcc
     1561 tctgttgcgg gtccgggaac cggctccgcc agcttgaaat gatcatactc ttccacatcg
     1621 cagaaataca gggatgaaaa aagctcctgc aaacagacta tctgcgcccc gcctgctgcc
     1681 gcctcccgga tcctgtccat cgccttttgc aggtttgagg ccttatcggc actgcagctc
     1741 atctgcacca ggccaacctt gatcttgctc atagttgcat ccgatcgggg gttttccgac
     1801 tggccgcaaa attacaggat taacctgaat tgagaaggtt acttatttac cacccgttga
     1861 aggccatcaa gtcgccggcg tcatttctta cgccccattt gcctcagaaa ggcgacatgg
     1921 gaagccactg cataccgcac gcggggaaac tcgatataga ccagcccgta ttcctggcag
     1981 gcctgccgga tgatcttgct gatcgcggga taatgaacgt gggatatctt ggggaagagg
     2041 tgatgctcga tctgaaagtt aagcccaccc accagccagc tgacgaccgg gttgcgggtt
     2101 gcgaaattcg cggtggtgcg aagctgatgg atggcccatt catcctccat ttttccggta
     2161 gcttcgtctg ctttcggaaa cgcggtggcc tgaacggtgt gggccagctg gaataccatg
     2221 ctcaaaacaa acccggctac catcgtgaaa atggaaaagc tgacgagcca atcataccag
     2281 cccagccggt agatcggcag aaaaatgaat aaaaaggcat gaaagacctt gaatccccaa
     2341 aaaacaaaat ggtccccgag gctcatcttc ttcaacggca tagtgccgat cttgttactg
     2401 aaatacttct ggtagtcgag gacgaagatc caaaaaatgt acaggactga atatagcacc
     2461 cagaaataaa gatgctggta ccggtgcagc cggtatcgct tttgcgcttc gctcatcctc
     2521 atccagggct gaatatcgat atcatcatcg acgccctcaa cgttcgtata ggagtggtgc
     2581 acgacattgt gcttcatatt ccacataaag ctgttgccgc ccaggatatt caatgagaaa
     2641 gccgccaggc tgttgaccca tttatatttg ctaaagctcc cgtgtgcgcc gtcatgcatg
     2701 acattgaacc cgatcgcggc gaccagtccg cccagcagca cgctttccag gatagcccaa
     2761 aacgcggccg gagtgaaaaa gacgagatga atataaataa agacaaaacc gaccatcagg
     2821 ataaccgcct tcagaaaaag atggtaatca ccggtcttcg ccttgcctac ttcttcaaaa
     2881 taattgctga tccgcttctt tagctcagta tggaaggact gcggaccgga ggaaaacttt
     2941 ggaagggaca tcgataaacg ggttgaaaat tgcatagtgg cacaaagtta ccggatttgg
     3001 cgcggacagg gatgttaatt tttcccggcc gccgggctat ttaacgcaaa tccgggccct
     3061 ttgttttgtc ctcaggtgtc cgcctgaaca aaagcatcaa aattgcgcac tccgaccacc
     3121 ttttcggtta aaaaaccttc cgcgtaggga acgccgatca tcttgcccag caactggtga
     3181 cgaccgatga tcgcattcag gaattcgggg ctcgaaatgt aggtttgggg ctcgtccgcc
     3241 tcgtatccgg tggaatagaa ttgtgatgaa taagccctga tcgactggag ctttgcctcg
     3301 aaaacaggtg aaatatcgta gacgaagctg ggatgggtgt accggtcctg aataaaatga
     3361 aaaagatact tgggccgcca gtgctcctgc gggcttcctc cttcgtccag cgtttctacc
     3421 ttccggagac cgcttaaaaa acatgcctcc cggatcagct gacccgcccg cccgtgatcg
     3481 ggatgccggt cttcgagcgc attgcccaga acgatctccg gccggtattt tcggatagcc
     3541 cggatcaaca accgttgatg ttcctcgtca ttcctgaaaa acccgtcctt catgccgaga
     3601 ttctcccggg catccaatcc cagtatcccg gcagctttgg ccgcctccag cgctctcagt
     3661 tccggcgttc cgcgcgtacc caactctccc cgggtcagat ccaccacacc cgtacgtttc
     3721 ccgtttgccc gttccatcat caaagtcccc gaacagccta gttctacatc gtccggatgc
     3781 acggcaatcg ctaatatgtc cagcttcatt tttgacttaa ttttcggcgc aaatctactc
     3841 tttttaacgt agcttcgcgg gtcgcatttt ttcagaaagt gcacaatttt aatcaaaatc
     3901 aggtagatat ggcatacgat ctgatcgtcc ttggcagtgg taccggcgga tatccgtcgg
     3961 ccatccgtgc cgcacagtta ggatttaaag tagcggtcat cgagaaagaa aacctcggcg
     4021 gggtctgcct taactggggc tgcatcccca ccaaggc
//
LOCUS       DATGKE010000011         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1550123, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000011 DATGKE010000000
VERSION     DATGKE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550123"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VKU83_00165"
     CDS             complement(<1..274)
                     /locus_tag="VKU83_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91987.1"
                     /translation="MIKAWFSILLAGAALTAAAQLPGPTGDPSGGKLLLPNGWSLTPA
                     GHSLPLGDLPLNIAVSSSRRYLAVTNNGQSTQTIQLIDAKKERVLDS"
     gene            complement(293..1000)
                     /locus_tag="VKU83_00170"
     CDS             complement(293..1000)
                     /locus_tag="VKU83_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLI91988.1"
                     /translation="MILAAGLGTRFKPWTDRHPKALAIVNGKSLLQRNIEYLQQSGIG
                     EVVVNVHHFAGQIIDALTANRGWGSAFMISDETDAVLETGGGLQKARPFLEGRTFVLI
                     NVDILTDMDLPAMIDDHFRHQPLATLAVTGRSTSRYFLFDDHDELCGWRNEATGEERM
                     ARTVLGAVVPKAFSGIHVIDPLIFQLMTRRGKFSMVDVYLDLADRYPIRAHDHSHSRL
                     IDVGRPGSAEDAARLFA"
     gene            complement(1046..>2383)
                     /locus_tag="VKU83_00175"
     CDS             complement(1046..>2383)
                     /locus_tag="VKU83_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLI91989.1"
                     /translation="EGPERIKEIIHWGTRFDKDAEGDFKLGKEGGHSEFRILHHKDVT
                     GKEIERALLDTVGKQRNIEIIKHWFVLDLITQHHLGYLVTKSTPDIECYGVYALDQHT
                     NKIVRILSKVTLLATGGNGQAYRTTTNPTIATGDGIAMVYRAKGRIENMEFIQFHPTA
                     LHEPGVSPSFLITEAVRGDGGILRNNRGDAFMEKYDPRKDLAPRDIVARAIDNEMKKA
                     GTEHVYLDCRHMDTVKFMEHFPNIYEKCRSIGIDVAVQQIPVAPAAHYSCGGIKVDEW
                     GRTSIAHLYACGECSSTGLHGANRLASNSLLEALVFSHRCYLDSVQQVPYIEFVSKIP
                     DWNAEGTTEPDEMILITQSLKELQWLMSDYVGIVRTQRRLLRASKRLDLLHEEIESLY
                     ETTAVSPQLCEVRNLITVGYLIVKEAQFRKESRGLHYNTDYPEKSPILQNIVL"
BASE COUNT          462 a          704 c          663 g          554 t
ORIGIN      
        1 tgctgtcgag gacgcgctcc tttttagcat cgatcagttg gatcgtctgc gtgctttgac
       61 cattattggt caccgccagg taccggcgtg aggagctgac ggctatgttc aatggcaggt
      121 cgccgagcgg aaggctgtgt ccggccggtg tcagggacca tccattgggc agcaacagtt
      181 ttcccccgga agggtctccg gtcggtccgg ggagctgcgc ggccgccgtc agcgcggcgc
      241 cagccaataa aatcgagaac caggctttta tcatattccg aaaattatgt ttttatgcga
      301 acagcctcgc ggcatcttcc gccgacccgg gccggccgac gtcgatcagc cgggaatggc
      361 tgtggtcatg cgcccggatc gggtatcggt ccgccaggtc gaggtaaacg tcgaccatcg
      421 agaatttccc gcgccgggtc atcagctgga agatcagcgg atcgatcaca tggatgccac
      481 tgaatgcctt gggtaccacc gcgcccagca cggtacgcgc catccgctcc tcgcctgtcg
      541 cctcattccg ccagccgcag agttcgtcgt ggtcgtcaaa caggaaatac cgcgaggtac
      601 tgcggccggt cactgccagc gtggccagcg gctggtgacg aaagtggtca tcgatcatcg
      661 cgggcaaatc catatccgtc aggatatcga cgttgatcag cacgaacgta cgcccttcca
      721 ggaaaggccg cgccttttgc aaaccgccgc ctgtttcgag caccgcatct gtctcatcgc
      781 tgatcatgaa cgcgctgccc cacccccggt tggcggtcag ggcatcgatg atctgacctg
      841 caaagtgatg cacattcacg accacctcgc cgatgcccga ttgttgcaga tattcaatgt
      901 tgcgttgcag caggctcttt ccgttgacaa tggccagcgc tttcggatgc ctgtcggtcc
      961 acggcttgaa cctcgtgccg agaccagcgg ccaatatcat tgcccgggaa attttggaag
     1021 aagatgccgt catgcgatgt acaggttata aaacaatatt ctgcagtata gggctctttt
     1081 ccggatagtc ggtgttgtag tgcaaacctc ggctttcctt cctgaattgc gcctccttaa
     1141 cgataagata acccacggtg atcaaattgc gcacctcgca cagttgcggt gataccgctg
     1201 tcgtttcata cagcgattcg atctcttcgt ggagaagatc cagccgtttg ctcgccctga
     1261 gtaaccgccg ctgggtccgg acgattccga catagtcgct catcagccac tgtaattcct
     1321 tgagactttg ggtgatcagg atcatttcgt cgggctccgt cgtgccctcg gcgttccagt
     1381 ccgggatttt gctgacgaat tcgatatagg gaacctgttg cacgctatcc aggtagcagc
     1441 ggtgtgaaaa taccagcgcc tccaacaacg aattgctcgc gaggcggttg gcgccatgaa
     1501 ggcccgttga cgaacactcg ccgcatgcat acaaatgcgc aatggatgtc cgcccccact
     1561 cgtccacctt gatgccgccg cagctgtagt gcgcggcggg cgcaaccggg atctgctgaa
     1621 ccgcaacatc gataccgatc gaccggcatt tctcgtaaat gttcgggaaa tgctccatga
     1681 acttcaccgt gtccatatgc cggcaatcga gatagacgtg ttccgtaccg gccttcttca
     1741 tttcgttgtc gatcgcccgg gcaacaatat cccttggcgc caggtccttt ctcggatcgt
     1801 atttctccat aaaagcatcc ccccggttat tgcgcaggat gccgccgtcg cctctcaccg
     1861 cctcggtgat caaaaaagaa gggctcacgc cgggctcgtg aagggcggta gggtggaact
     1921 ggatgaactc catgttttcg atccggccct ttgcccgata gaccatggct attccgtccc
     1981 cggtggcaat cgtcgggttg gtggtggtcc ggtaagcctg cccgttgccg ccggtcgcca
     2041 ataaggtgac cttggacagg atgcggacga tcttgttcgt gtgctgatcg agcgcgtaga
     2101 ccccgtaaca ttcgatatcc ggcgtggact tggtaacgag gtagcccagg tggtgctgcg
     2161 tgatcaggtc gagcacgaac cagtgtttga tgatctcgat gttcctttgt ttgccgacgg
     2221 tatccagcag ggcccgttcg atctccttac cggtgacgtc cttatgatgc agtatccgga
     2281 actccgaatg gccgccctct tttcccagtt tgaaatcgcc ttcggcatcc ttgtcgaacc
     2341 gcgtacccca atggatgatc tcttttatcc gttccgggcc ctc
//
LOCUS       DATGKE010000012         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_100062, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000012 DATGKE010000000
VERSION     DATGKE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100062"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VKU83_00180"
     CDS             complement(<1..390)
                     /locus_tag="VKU83_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91990.1"
                     /translation="MIAALDIQVTKTTRSRLSEIDFNNLPFGKYFTDHMLEVDYANGQ
                     WQKAAIRPYQHLSLSPALAALHYGQAIFEGIKAYKDRDGHPFIFRPFDNFRRFNVSAE
                     RMNMPEVPEEIFMEGMRRLVELDRDWIP"
     gene            complement(492..1418)
                     /locus_tag="VKU83_00185"
     CDS             complement(492..1418)
                     /locus_tag="VKU83_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91991.1"
                     /translation="MNKKGIFRVLVFLVALILCDSVMGMVARMLFYSQKSGKYARLTY
                     TVKSDTSAIVVLGSSHALAHFIPDMIQDSLHESAFNYGANGQKLLFSRALYEIRKRRS
                     EPAMVLLNVDADWFFDKRGQEERMSDLYPYYDQAGDIILRDFSSRDRFIERLKFLSKT
                     FPYNSTIVHVIRYKLKPQHDFNGYEPLFGAVDSAQFARELHSETRKVDPALPQPDGEL
                     MSLFTQFIGEMQKDRIRLVIVFSPELLKPDKFERAMDEKIKNICDSTGTTVLDFSTSD
                     FFKITGSCFTIFLISMIPAPGSLRECSLTASG"
     gene            complement(1421..2818)
                     /locus_tag="VKU83_00190"
     CDS             complement(1421..2818)
                     /locus_tag="VKU83_00190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HLI91992.1"
                     /translation="MLFNSFTFVFFFLTVTTLYYLLPFKFRWALLLVASCIFYMAFIP
                     IYIFILLGTIVIDFFAGLAIEKTRSRLRRKALLTVSILSTCMVLCVFKYYNFIVGNYN
                     DLAKALHWNYSVNILRIILPIGLSFHTFQSLSYVIEVYRGHQKAEKDFGIYSLYVMYY
                     PQLVSGPIERPQNLLHQFRREVKFDWNIAAAGLKTMCMGYFMKLVVADRCSIYVNAVY
                     NNVHAHGGLTFIAATFFFAFQIYADFAGYSLIAIGASQTMGIQLMTNFRTPYASNSLS
                     EFWRRWHISLSTWFRDYLYIPLGGSRDSKIKTYRNLMITFVVSGLWHGANWTYVIWGA
                     YHGAYLIIEQIAGKGKKRRYPQIWVFAVVCFGWIFFRSNTVQDSFYIIWSIFTHPGTL
                     FIPKNQDVTAPIYALIAIGLLLLVENRSRVSTRLAIPGKPVYAYVNMTFYISLILGII
                     YLGVFNGGQFIYFQF"
BASE COUNT          829 a          728 c          726 g          700 t
ORIGIN      
        1 cgggatccag tcgcggtcaa gctcgaccag ccgccgcatt ccctccataa agatctcctc
       61 cggaacctcc ggcatgttca tccgctccgc cgagacgttg aacctgcgga aattatcaaa
      121 aggccggaag ataaagggat ggccatcgcg gtccttatag gctttgatgc cctcgaaaat
      181 ggcctgtccg taatggagtg cggccagcgc cggcgacagg ctgagatgct gataggggcg
      241 gatagccgcc ttttgccact ggccgttggc gtagtcgacc tccagcatat gatcggtaaa
      301 atacttgcca aaaggaaggt tgttgaaatc gatttcgctc aaccggcttc tcgtagtttt
      361 cgtgacctga atatcaaggg cggcaatcat ggcgactaaa tttctaggga caaagaaaca
      421 aacaatcttt gggtcagcca aatcgaaaac taacaattgt tagttaagtt tatactcgct
      481 gattgctggc ctcaacctga tgctgtcaat gagcattccc gtaaagatcc tggcgccgga
      541 atcatcgaga tgagaaaaat cgtgaaacag gagcctgtta ttttgaaaaa gtcgctggtt
      601 gaaaaatcga gcacagtggt gccggtgctg tcgcagatat tctttatttt ttcgtccatt
      661 gcccgttcaa acttgtccgg cttgaggagc tcgggcgaaa aaacgatgac cagcctgatc
      721 cggtcttttt gcatctcgcc gataaattga gtgaacagac tcatcagctc accgtccggt
      781 tgaggcaacg cggggtcgac cttccgggtt tcagaatgaa gctcacgggc aaattgggcg
      841 gaatctacag cgccgaacaa gggttcgtac ccattgaaat catgttgcgg cttcagttta
      901 tacctgatga catgaacgat ggtcgaattg tacggaaagg tcttcgagag aaattttaac
      961 cgttctatga acctgtcccg gctgctaaaa tcccttagga taatatcgcc cgcctggtca
     1021 taatatggat aaagatccga catccgctct tcttgaccgc gcttgtcaaa gaaccaatcc
     1081 gcatccacgt tcaggaggac catcgcgggc tccgagcgcc ttttgcgaat ttcataaaga
     1141 gccctgctga ataatagttt ttggccattg gctccataat tgaaagccga ctcatgaagg
     1201 gagtcctgga tcatatccgg aatgaaatgc gccagggcgt gtgaacttcc cagtacgact
     1261 atcgctgaag tatcggactt taccgtgtag gtcagccttg catattttcc gcttttctgc
     1321 gaatagaaca gcatcctggc aaccataccc ataacggagt cgcacaggat gagggcaacc
     1381 aaaaaaacga gaacccggaa aatgcccttt ttattcataa ctagaattga aagtaaatga
     1441 actgccctcc gttaaacacg cctaaataga taatacccag gatcagcgag atgtaaaagg
     1501 tcatgttgac ataggcatat accggcttgc ccgggatcgc caacctggtc gaaacgcgcg
     1561 accggttctc tacgagcaac agcagtccta tggcgatcag tgcatagatc ggcgccgtga
     1621 catcctgatt ctttggaata aataatgtcc ccggatgagt gaatatcgac cagataatat
     1681 agaatgaatc ctgtacggtg ttcgatctga aaaatatcca gccaaaacag accactgcga
     1741 atacccagat ctgcgggtat cggcgttttt tccccttgcc tgcgatttgc tcgatgataa
     1801 ggtaggctcc gtggtacgcc ccccagatca cgtacgtcca gttcgccccg tgccaaagac
     1861 cgctgaccac gaacgtgatc atcaggttcc gataagtttt gatcttcgag tcacggcttc
     1921 cgccaagcgg gatatacagg taatcccgga accaggtcga caacgaaata tgccatcttc
     1981 gccaaaattc cgacaagctg ttggaagcat aaggcgtccg gaaattcgtc atgagctgta
     2041 tgcccattgt ttgtgacgcc ccgattgcga tcaacgagta cccggcaaaa tcagcgtaga
     2101 tctggaaggc aaaaaagaag gtagcggcaa taaaggtcag tccgccgtgg gcatgcacgt
     2161 tattgtagac tgcgttgaca tagatcgagc agcggtccgc aacgaccagc ttcatgaaat
     2221 accccatgca catcgttttc aggccggcgg cagcgatatt ccagtcgaat tttacctcgc
     2281 gcctgaactg gtgaagcagg ttttgcggcc tttcaatggg tcccgagacc aactgggggt
     2341 aatacatcac atacaggcta tagatcccaa agtctttttc cgctttttga tggccccggt
     2401 agacttctat tacgtaactt aagctctgga aggtgtggaa agatagcccg atgggcaaaa
     2461 ttatcctcaa tatatttacg ctgtaattcc agtgcagcgc ttttgcaagg tcattatagt
     2521 tgccaacgat gaaattgtaa tatttgaaaa cgcatagcac catgcaggta cttaaaatgc
     2581 tgacagtcaa tagggctttt cgtcgcaacc ggctccgggt cttctcgatg gcgagaccgg
     2641 cgaaaaaatc gatcacgatg gtaccgagaa ggataaaaat gtaaatcggg ataaaggcca
     2701 tgtaaaagat gcaactggcg accagcaaca aggcccagcg aaacttgaaa ggcagcagat
     2761 aatataaggt cgtgacggtt aggaagaaaa atacaaacgt gaatgagtta aatagcatac
     2821 aggatcggtt aacggaagca gccaacaata ttaccaaaag gttgccaaat accggctact
     2881 tttgtcctgc tgcaaaatag ctcatgcagc cccccagctc ttaagaaatg gaccttaaca
     2941 acatcaagct ttctgatctc gtcatccgcg aatggtacaa gga
//
LOCUS       DATGKE010000013         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_721545, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000013 DATGKE010000000
VERSION     DATGKE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721545"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VKU83_00195"
     CDS             <1..518
                     /locus_tag="VKU83_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91993.1"
                     /translation="HQLITQPSLKEQVVVSKQQWPSTTILKNVTFQFIYHNPTHFFGV
                     KKIWIDNHHQVPCSDLEKTIVDCLFKPNYAGGITEVAKAIQMAKNRIQFDKLLVYVIQ
                     FRSKAVAKRLGFLLEVLDIATPIIESLQQQKSSSYVVLDTELPDAGPFVSRWGIRQNL
                     DTATIKSAIAT"
     gene            515..1324
                     /locus_tag="VKU83_00200"
     CDS             515..1324
                     /locus_tag="VKU83_00200"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLI91994.1"
                     /translation="MIKTREIQQKAQRDGVRDTQIEKDYILSWILWGVARHQALAQCL
                     VFKGGTALKKIYFEDYRFSEDLDFTLIDQQLSNDQLLTWFDQSFSTVREAANIPFSLQ
                     PHQEHQDGGLNFYIQYVGPLGGLGANKTVKVDISRNEQLVYAPIAKQVITNYSDLEQH
                     ELLCYSLEEVLVEKLRSVMQRMQARDFYDIWYLLEVHGLQPHFHYNEFCRKCAYKGMR
                     PTDLLQKLADRLPQYKARWKNSMNDQIRDLPDFDKVAREVQKHLRKMPFDS"
     gene            complement(1512..1884)
                     /gene="ssrA"
                     /locus_tag="VKU83_00205"
     tmRNA           complement(1512..1884)
                     /gene="ssrA"
                     /locus_tag="VKU83_00205"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1987..2991
                     /locus_tag="VKU83_00210"
     CDS             1987..2991
                     /locus_tag="VKU83_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI91995.1"
                     /translation="MKMPLLSTLLVLLASGLFAPSPKDYRITNTFHIKSGGGYDYTTV
                     DPSSNRLYLSHGTQVNVIDKTTGDSIGVIKTDHDVHGIALVHALGKGYITNGSANSAV
                     VFDLKTLQILGHVPTGPFADGILYDDFSNKVITCNGRGKNMTVIDPKTDKVVATIQLT
                     GWPETAQSDGKGRIYVNNAEKSEMDVIDTRNFQVIHSWPIAPGHRASGLAIDRSTMRL
                     FAGCDNKLLVVMDAVTGKVVRTLPIGDGCDAVGFDNGLKTVYSSNGEGTLTIIKEHSA
                     NDLSVVENLKTKPGARTNAVDPGTHAVYLPTGEFEPKKTGEFRPKLIPGTFQVLVVKP
                     "
     gene            2997..3248
                     /locus_tag="VKU83_00215"
     CDS             2997..3248
                     /locus_tag="VKU83_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HLI91996.1"
                     /translation="MITGEAFARMALSFPGTGQVPHFDRIGFKVAGKRMFSTYLEKDN
                     TANVFLTPQEQAVFCQMDKTGNEPARNPGSARRRGARRQ"
BASE COUNT          897 a          889 c          782 g          727 t
ORIGIN      
        1 ttcatcaact gattacacaa ccttccctaa aagaacaggt tgtcgtttct aaacaacaat
       61 ggccatccac aaccatcctt aaaaatgtca ctttccaatt catctaccac aatcccaccc
      121 acttttttgg cgtcaaaaag atttggatag acaatcacca tcaggtcccc tgttccgatc
      181 tggaaaagac aattgtcgat tgcctcttta aacccaatta tgcgggaggc atcacagaag
      241 tagccaaagc tatccaaatg gcaaaaaacc ggatacaatt tgataaactg ctggtctatg
      301 ttattcaatt caggtcaaaa gccgttgcca aaaggttggg atttctgctt gaagtactcg
      361 atatagctac cccgatcatc gagtctctgc agcaacaaaa gtcttcctcc tatgtcgtgc
      421 tggataccga gcttcctgat gcaggacctt tcgtcagccg ctggggcatt cggcaaaatc
      481 tcgatacagc aaccattaaa tcagctatcg ccacatgatc aaaacaaggg aaattcaaca
      541 aaaagcccaa cgagatggcg ttcgggatac ccaaatagaa aaggattata tcctttcgtg
      601 gatattgtgg ggcgtggcac gccaccaggc gcttgcgcaa tgcctcgtct ttaaaggcgg
      661 aaccgccctg aaaaagatat atttcgagga ctatcgcttc tcggaagacc ttgactttac
      721 gttgatcgac cagcagctgt caaacgatca acttcttacc tggtttgacc aatcgtttag
      781 cacggtccgc gaagccgcga atattccctt ttcgctccaa ccgcatcagg agcaccagga
      841 tggcgggctc aacttctaca tccaatatgt cggaccattg ggcggtcttg gtgctaacaa
      901 aaccgtcaag gttgatattt cccgcaatga acaactggtt tacgccccaa ttgcaaaaca
      961 ggtgatcacc aattacagcg atctggaaca acacgaatta ctttgttatt cccttgaaga
     1021 ggtcctggta gaaaaactaa gatcggtgat gcagcggatg caggcgcgag atttttatga
     1081 tatctggtat ctcctcgaag tgcatggact acaaccgcat tttcattaca acgagttttg
     1141 ccggaaatgc gcttataaag ggatgaggcc aaccgatctc cttcagaaac ttgcggatcg
     1201 actgcctcaa tataaagccc gatggaaaaa ctctatgaac gaccagatcc gggatttgcc
     1261 cgattttgac aaagtcgccc gggaagtgca aaagcacctc cgaaaaatgc cctttgatag
     1321 ctgattgaaa accagtacat tttctttacc attgccggac aaaagacaat gcactttctt
     1381 cctaactggg aaaagctgaa gaaagagttg aaccggcttc ccgtaagtca tgcagactgg
     1441 gcttattgat cggtgacaaa atcgtcctaa aacaaaaaaa gccatcgcaa attgttaatt
     1501 tacaatgact taagtggagc tgaggggagt cgaacccctg tccaaacatc acctccgaaa
     1561 gctttctaca tgtttattcc ggcattcgtt gtcgggaaat ggctggaacc ggacgaaccg
     1621 accatgtcct tagctgcatc gtcttaagca acggtcacag ccttccgttg cagcatcctg
     1681 tatttatttt gagtcggcag cgcagcttgg caacaggcga acctgccagc gcggcccaaa
     1741 tgactatcta atcactgatt aggcagccat ggcatactga gtattgccat ttgtagtttg
     1801 aatattcaga ttaacgtgct aattatccaa cgcacgacat gcttacacct tcaaagatct
     1861 atgctgtcaa aaccgggcag ccccagcctg caaagatacg aaaaattccc gggcctgtac
     1921 cgtcgaccgg ccggaaatcc aaaatttaaa caaaatcgct cgtcacctta aaggaaattt
     1981 cctcaaatga aaatgcccct tctctcaacg ctgctcgtcc tcctcgccag cggccttttt
     2041 gccccgtcac cgaaagacta ccggatcacc aacaccttcc atatcaaaag tggcggcggt
     2101 tacgactata cgacggttga tccttcctcc aaccgcctgt atctgtccca cgggacccag
     2161 gtcaacgtta tcgataagac gaccggggat tcgatcggcg tcattaaaac cgaccacgac
     2221 gtccatggca ttgcgctggt acacgcgctc gggaaagggt atatcacgaa cggatcggct
     2281 aacagcgcgg tggtttttga tctgaaaaca ttgcagattt tgggtcacgt gccgaccggc
     2341 ccgttcgccg acggcatcct gtatgacgat ttctcgaaca aggtgatcac ctgtaatggc
     2401 agaggcaaga acatgacggt gatcgacccg aagaccgata aggtggtcgc caccattcaa
     2461 ctgacgggct ggccggaaac cgctcaatcc gacgggaagg gcaggattta cgtaaataat
     2521 gccgaaaaga gcgaaatgga tgtcatcgac acccggaatt tccaggtcat ccacagctgg
     2581 ccgatcgcgc cgggccaccg ggcatcgggc ctcgcgatcg accgttcgac catgaggtta
     2641 tttgccgggt gcgacaacaa gctcctcgtc gtaatggacg cggtcaccgg aaaggtcgtg
     2701 cggaccttgc cgatcgggga cggttgcgat gcggtcggct ttgacaacgg cctgaagacc
     2761 gtatattcct ccaacggcga gggaacgctc accattatca aagaacattc cgcaaacgac
     2821 ctgtccgtgg tggagaacct gaagaccaaa ccgggggcgc gaaccaacgc ggtagatccc
     2881 ggcacccacg ccgtctatct cccgaccggg gaattcgagc cgaagaagac gggcgaattc
     2941 cgaccgaaac tgatcccggg aacctttcag gtcctggtgg tgaaacccta acgcccatga
     3001 tcaccggaga agcctttgcc cggatggccc tctccttccc cgggaccggg caggtgcctc
     3061 atttcgaccg gatcgggttc aaagtcgccg gcaagcgcat gttcagtacc tacctggaaa
     3121 aggacaatac ggcaaacgtg tttctgacgc cgcaggagca ggccgttttt tgccagatgg
     3181 acaagacagg taatgaaccg gcacgaaacc cgggatccgc gcgccggcgc ggcgcgcggc
     3241 ggcaatgaaa ttcctcagga ccggcctggc acgaaaatta cattgcgata gcaaa
//
LOCUS       DATGKE010000014         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_202870, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000014 DATGKE010000000
VERSION     DATGKE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202870"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..467
                     /locus_tag="VKU83_00220"
     CDS             99..467
                     /locus_tag="VKU83_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLI91997.1"
                     /translation="MSIQSVVLLLAVGVLAGMLSGLVGLGGGVIIVPALVYLLGFSQH
                     QATGTSLGILLLPAGIFAVMNYYRKGYIDVRVVLLIFAGFLVGGYLGSKISLNIPEGT
                     LKKIFAVILVLIAGRVLFLD"
     gene            complement(453..1226)
                     /locus_tag="VKU83_00225"
     CDS             complement(453..1226)
                     /locus_tag="VKU83_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HLI91998.1"
                     /translation="MSPAICCVPVSPLRADPSHRSEMVSQLVFGEYAGILEQTGRDWC
                     RVRGRFDGYEGWCQPSHLVAAGDSAAEGQGLTPDWVTAIEYNGVPMQVPMGSQLTGLT
                     AGVARWGPNEIRYSGQVWDPLRAARDPASVRKLTRCFLNTPYLWGGKSIFGIDCSGFS
                     QTVFRFLGIPLLRDAALQAGQGEPVGFLEETRTGDLAFFDNDEGRITHVGVLLSPHEI
                     IHSSGKVRIDRIDNMGIVNVDSNQRTHHLRLIKRVLVQK"
     gene            1374..1949
                     /locus_tag="VKU83_00230"
     CDS             1374..1949
                     /locus_tag="VKU83_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI91999.1"
                     /translation="MPKRPHDDMSDQELLDLFFSTGNNDWLGILLPRYTLLLLGVCMK
                     YLKNEEEAKDCVQQIFVKVITELRKYRVDYFKSWIYTVARNHCLMKLRDQHGQPAPLS
                     ETMAAAWDEEPGRNSHLEKEQWLEWMSQSLGELSPEQKLCVTLFYLEKRSYQEISLKT
                     GYTPMQVKSYIQNGKRNLKLSMEKKAREKTS"
     gene            1951..>2281
                     /locus_tag="VKU83_00235"
     CDS             1951..>2281
                     /locus_tag="VKU83_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92000.1"
                     /translation="MNKDLLNILANSNKDIDNQQLMDYLSGKLSAESLHDVERSMADN
                     AFLNDAVEGLQQIGNRHDLQAYVDELNASLHRNMAKKKERRLKRRFLDSPWTYVAIGM
                     VIVFCLMA"
BASE COUNT          540 a          617 c          639 g          485 t
ORIGIN      
        1 gtggaagaat tcggggacaa aacgaccgcg cttaaggtca ttgaagaggc gatgcggctc
       61 tatgatgaga cgtaccggag aaaataaccc tcctttatat gtcgatccaa tcggtagtgc
      121 ttttgctggc cgtcggagtc ctggccggga tgttgagcgg gctcgtcgga cttggcggcg
      181 gcgtgatcat cgttcccgca ctcgtctatt tgctgggttt cagtcagcac caggcgacgg
      241 ggacctcact cgggatactc ttgttgccgg ccggaatttt tgcggtcatg aactactaca
      301 ggaagggcta tatcgatgtc cgcgtcgtgc tgctgatctt cgccggcttc ctggtgggcg
      361 gctatcttgg cagcaagatc tccctgaata ttccggaagg aacactgaaa aaaatcttcg
      421 cggtcatcct cgtgctgatc gccggaagag ttttatttct ggactaaaac ccgtttgatc
      481 agccgcaggt gatgcgtccg ctgattgctg tccacattga cgattcccat attgtcgatt
      541 ctgtcgatcc gcacctttcc tgaagaatgg atgatctcgt gcggactaag caggacgccg
      601 acgtgcgtga tccggccttc atcattgtcg aaaaaggcaa ggtcgccggt ccgggtctct
      661 tccaggaaac ctaccggttc gccttgtccc gcctgcaaag cggcgtcgcg aagcaacgga
      721 atgcccagga acctgaaaac cgtttgggaa aaaccgctgc agtcgatgcc gaaaatgctt
      781 ttgccgcccc acagataagg cgtattcaga aaacaccgcg tcagcttccg gacggacgcg
      841 ggatcgcggg ctgcgcgcaa cgggtcccat acctgccccg aataccggat ctcattcggc
      901 ccccagcggg caacgcccgc cgtcaagcct gtgagctggc tgcccatcgg gacttgcatc
      961 gggaccccgt tgtattcgat ggccgttacc cagtccggcg tcaatccctg tccttcggcg
     1021 gccgaatcgc cggctgctac caggtgcgag ggttggcacc agccttcgta gccatcgaac
     1081 cgtcccctca cccggcacca gtcccggccg gtctgctcca atatcccggc gtactcgcca
     1141 aaaacaagct ggctaaccat ctcggatcga tgggaaggat ctgccctgag cgggctgacg
     1201 gggacgcaac aaattgccgg actcattccg aaaattaaag aatgggactc ctttggcaaa
     1261 attttatggt ctgccggctg ttagaattgg gttgaagccg cccggcgccc cgtagggaga
     1321 caagatgctt gtgaactttt ctgccgcgcg catcttatgt atattgcaat cacgtgccaa
     1381 aacggcctca cgacgatatg agcgaccagg agctgttgga tcttttcttc agcaccggga
     1441 acaatgactg gctgggtatc ctgcttcccc gatacaccct cctgctgctg ggggtttgca
     1501 tgaaatacct gaaaaatgaa gaggaggcaa aggactgcgt gcagcagatc tttgtcaagg
     1561 tgataaccga gctcaggaag taccgggtgg actatttcaa atcctggatt tatactgtcg
     1621 cccggaatca ttgcctgatg aagcttcggg accagcacgg tcagccggcg ccgctctcgg
     1681 aaacgatggc ggctgcatgg gatgaggagc ctggtagaaa cagccacctg gaaaaggaac
     1741 aatggctgga atggatgagc cagtccctcg gcgaactgag cccggaacaa aaactctgtg
     1801 taactttgtt ctacctcgag aaacgatcgt accaggaaat atcgttaaaa acggggtaca
     1861 ccccaatgca ggtgaagagc tacattcaga acggcaaacg gaacctgaag ctgtcaatgg
     1921 aaaagaaggc ccgagaaaaa acaagctgac atgaacaagg acctgctcaa catattggct
     1981 aacagtaaca aggacatcga caatcagcag ctgatggact atctgagcgg caaactctcc
     2041 gcagaatccc tccatgacgt cgagcggtcc atggcagata atgccttcct caacgatgcg
     2101 gtcgaagggc tgcagcagat tgggaacagg catgatctcc aggcatacgt cgacgagctg
     2161 aatgcaagcc tgcaccgcaa tatggcgaaa aagaaggagc gccgcctgaa aagacgcttt
     2221 ttggattcgc cgtggaccta tgtggctatc gggatggtga tcgtcttctg tttgatggca
     2281 t
//
LOCUS       DATGKE010000015         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_509258, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000015 DATGKE010000000
VERSION     DATGKE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509258"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VKU83_00240"
     CDS             complement(<1..504)
                     /locus_tag="VKU83_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92001.1"
                     /translation="MSFPFLKPVCLAIICWQAASGSAPAQGTPRRTLLALSKADHTLA
                     IVDPGNLQVLARIPVGSDPHEVVASSDGRTAFVSIYGGGSLHELDILDLDAQKPKQQF
                     DTRPLLGPHGLDFAEDELWFTAEGSKAVGRFDPATGRVDWVMGTGQDRTHMIYVASGG
                     KRVYTTNV"
     gene            complement(551..1042)
                     /locus_tag="VKU83_00245"
     CDS             complement(551..1042)
                     /locus_tag="VKU83_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HLI92002.1"
                     /translation="MIKKILIVVFLILVVIQFIRPEKNRSGNTDHNIATAYNVPENVN
                     KILQASCADCHSNKTEYPWYAEVQPVGWWLSNHVTNGKRHLNLDNFTTMRIAFQKKRM
                     EDCIEQLKQGDMPLGSYTLIHRYAILSNAQKDTLINWCQGIVDEIKAKYPPDSLVLPK
                     RRR"
     gene            complement(1091..1540)
                     /locus_tag="VKU83_00250"
     CDS             complement(1091..1540)
                     /locus_tag="VKU83_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92003.1"
                     /translation="MKKSILVLALAILGFAGSAFAGTKDPSVPGRITDQFSNQFAKAT
                     DVQWQMGKNFFKATFEDWGRTLFAFYAFNGDLMGVASNLSPAGLPGRLKTQLHRDFAG
                     YWITDLFSYGNQNERGMVVTIENPDRIVVLKALGKGNWIVYRTTIKN"
     gene            complement(1668..>3931)
                     /locus_tag="VKU83_00255"
     CDS             complement(1668..>3931)
                     /locus_tag="VKU83_00255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HLI92004.1"
                     /translation="YFLTVYDIVRYARNHGILCQGRGSAANSTICYCLGITSVDPTKF
                     DLLFERFISPARNEPPDIDVDFEHERREEVMQYVYNKYGRDRAAIVATVTQVHQKGAI
                     RDVGKVMGLSPDTVDRLAKSVWEYGEVLDDKRVAEQGLDPSDTHLQKVLNLTAQYMGF
                     PRQLGQHTGGFVITQGKLSDLCPILNARMEDRTNIEWNKDDIDILGFMKIDVLALGML
                     TCIRKAFDLARDHYGLNWTLANIPQDDPQVYRMVSAADTIGVFQIESRAQQSMLPRLR
                     PETFYDLVIEVAIVRPGPIQGDMVHPYLRRRNGEEAVTYPSKELQEILGRTLGVPLFQ
                     EQAMKIAIVAAGFTPAEADGLRRSMATFKAHGLVTNFKQKLTDGMRRNGYTAEYAEKV
                     FRQIEGFGSYGFPESHAVSFALLVYVSAWIKCYYPDIFACAILNSLPMGFYQPAQLVI
                     DARKHGVVFRPVDVNHSQWDNTLEDEEGSYFSVRLGFRHITGVKEEDMLLLIAGRIRP
                     YHSVHSLQDAGLTVSCLEKLADADAFLSLGLDRRQALWEVSALSDRPIGIFRDQEAES
                     THETGIDLPPISLSEHVFRDYATMSLSLKAHPVHFLRERLHRLGNIAAAELLSHRNGA
                     FVKVAGIVLVRQRPGTASGVCFITLEDETGQFNLIVWPNLFEKYRKEILGAKILMVEG
                     HLQIEKEVVHVIVQRCHNLNRLLVHLEDQDGPGRERNMPRDDPQAGTQRVAPTRSGDV
                     MQASLFPEARNFR"
BASE COUNT          858 a         1083 c         1062 g          928 t
ORIGIN      
        1 aacattggtt gtatagaccc gtttcccgcc ggacgcgaca tagatcatat gcgtccggtc
       61 ctggccggtg cccatcaccc aatccacgcg gccggttgcc ggatcaaaac gcccgacggc
      121 cttcgatcct tccgccgtga accaaagttc atcttcggca aagtccagtc catgcgggcc
      181 gagcaggggc cgggtatcaa actgctgttt tggtttctga gcatccaggt cgaggatgtc
      241 gagctcgtga aggcttccgc ctccatagat agagacgaag gccgtccggc cgtccgacga
      301 cgcgaccact tcatgcggat cggaaccgac cggaatccgc gccagcacct gcaaattgcc
      361 cggatccacg atggccaagg tgtgatccgc cttggaaagg gccagcagcg tgcgcctcgg
      421 cgtaccctgg gcgggagcgg agccggaagc tgcctgccag cagatgatcg caaggcagac
      481 cggcttaaga aatgggaaag acataatgta aaatttatga acagtaaaat cgaatctgca
      541 aggaatgccc tcaacgccgc cgcttcggca acacgaggct gtccggcggg tatttggctt
      601 tgatctcatc gacaatcccc tggcaccagt tgatcagcgt gtctttttgc gcgttgctca
      661 ggatggcata ccggtggata agcgtataag aaccaagggg catgtcgccc tgtttcagct
      721 gctcgataca gtcctccatc cttttctttt ggaaggcgat ccgcatggtc gtgaaattgt
      781 cgaggttcaa atgccgcttc ccgttcgtga cgtggttgct caaccaccaa ccgaccggtt
      841 ggacctccgc ataccaggga tactcggttt tgttcgaatg acaatcggca caggatgctt
      901 gcaggatctt attgacattc tccggaacat tataggcggt tgcgatattg tggtccgtat
      961 tgccggaccg gttcttttcc ggacggataa actggatcac gacaagaatg aggaagacga
     1021 caatcaggat tttctttatc atatccgacg gggtttggga caaagatatt ttgtgaacgg
     1081 attcaaggca ttaatttttt atggttgtac ggtatacgat ccagttgcct ttaccgaggg
     1141 ccttcaggac cacgatccgg tcagggtttt cgatcgtaac gaccatgccg cgctcattct
     1201 ggttgccata gctgaagagg tcggtgatcc agtagccggc gaaatcccga tgcagctggg
     1261 ttttcaggcg tccggggagt cctgcggggg agagattgga ggcgacgccc atcaggtcgc
     1321 cgttaaaggc atagaatgcg aacaaggtcc ggccccagtc ctcgaaagtt gctttaaaga
     1381 aattctttcc catttgccac tggacgtccg tggccttcgc gaactggttg ctgaattggt
     1441 cggtgatccg cccgggtacg ctgggatcct tggttccggc aaaagcgctc ccggcaaaac
     1501 cgaggatcgc aagcgctaat actaaaattg actttttcat ggctgtaagt ttaatactgt
     1561 ttgttttgat ttgacgtcac aaaactaacc tgcgttcttt cccctttttg gcaaatgcgg
     1621 tgaacagggt aaattttgcg ttgaaccgca tgaatccgtc cggaatctca tctgaaattc
     1681 cgcgcttccg gaaacaggct tgcctgcatc acgtcaccgg acctggttgg agccaccctt
     1741 tgggttcctg cctgcggatc gtccctcggc atgttacgct ccctccccgg cccatcctga
     1801 tcctccagat gcaccaggag cctgtttaga ttatgacagc gctggacgat cacatggacc
     1861 acttcttttt ctatttggag atgtccttct accatcagga tctttgctcc gaggatctct
     1921 ttccggtatt tttcaaacag gttcggccag acgatcaggt tgaactggcc ggtctcgtct
     1981 tccagcgtga tgaaacaaac cccgctcgct gttcctggcc gttgtctgac caagacgatg
     2041 cctgccacct tgacaaaggc cccgttccgg tgactcaaca gctcagcggc agcgatattc
     2101 ccgagcctgt gcaaccgttc gcgaagaaaa tgcaccgggt gcgccttcag cgataaggac
     2161 atggttgcat aatcccggaa gacgtgctct gataacgata tgggtggcag atcaatgcct
     2221 gtctcatggg tactttcagc ttcctggtcc cggaatatcc cgataggccg gtcggataag
     2281 gcggagactt cccacaaggc ctgacgccgg tccaggccga gagaaagaaa ggcatcggca
     2341 tcggccagtt tttcgaggca gctcaccgtt aatcctgcat cctgcaatga atggacggaa
     2401 tgatacgggc ggatcctgcc cgcgatcagc aggagcatat cctcttcttt tactcccgtg
     2461 atatgcctaa aacccaggcg caccgaaaag tagctgccct cttcatcctc cagcgtatta
     2521 tcccattgcg aatggttgac gtctacgggc ctgaagacaa ccccgtgttt cctggcatcg
     2581 atgaccagct gggccggttg ataaaagccc atcggcaggc tgttgaggat agcgcaggca
     2641 aatatgtcgg ggtagtagca tttgatccag gccgagacat aaaccaggag ggcgaagctc
     2701 accgcgtggc tttccgggaa gccatagctt ccgaatcctt cgatctgccg gaatactttt
     2761 tcagcatatt ccgcggtata tccgttgcgc cgcatgccgt ccgtcagttt ctgtttgaaa
     2821 ttcgtaacca gtccatgtgc cttgaacgta gccatcgatc tccggagtcc atccgcttcg
     2881 gccggcgtaa aaccggctgc aacgatggcg atcttcatcg cctgttcctg aaagagcgga
     2941 accccaagcg tgcgaccaag gatttcctgg agctctttag acggataggt taccgcttct
     3001 tcgccattcc gccgtcgcag gtaagggtgg accatatcgc cctggatggg cccgggccgg
     3061 acaatggcga cctcgatcac gaggtcataa aaagtctccg gccgcagccg gggcagcatt
     3121 gactgctggg cgcgggattc gatctggaag actccaatgg tatcagcagc gctgaccatt
     3181 cgatagacct gtgggtcgtc ctgtggaata ttcgcgagcg tccagttgag cccgtaatgg
     3241 tcacgggcca gatcaaaagc cttccggatg caggtaagca tccccagcgc caacacgtcg
     3301 atcttcatga atccaaggat atcgatatcg tccttgttcc actctatatt ggtgcggtct
     3361 tccattctcg cgttgaggat agggcagaga tcggacagtt taccctgggt gatcacaaac
     3421 ccgccggtat gttgtccgag ctgccgggga aaacccatat actgcgccgt caggttgagc
     3481 accttctgca aatgggtatc gctcggatcg agaccttgtt ccgccacccg tttatcatcc
     3541 agaacctcgc cgtattccca gaccgatttg gccagcctgt cgacggtatc cggagaaagt
     3601 cccatgacct tgccgacgtc gcggatggcg cctttttgat gtacctgggt gactgttgcg
     3661 acgatggctg ccctgtcccg gccatatttg ttgtacacat actgcatgac ttcctcccgg
     3721 cgttcgtgtt cgaaatcgac atcgatatcc ggtggttcat tcctggccgg ggaaataaaa
     3781 cgttcgaaga gcagatcgaa ctttgtcgga tccactgaag tgatgccaag gcaataacag
     3841 atggtcgagt ttgctgcgga gccgcggccc tggcacaata ttccatgatt tctcgcatac
     3901 cggacgatat cgtagaccgt aagaaaatag g
//
LOCUS       DATGKE010000016         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_927990, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000016 DATGKE010000000
VERSION     DATGKE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927990"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..170
                     /locus_tag="VKU83_00260"
     tRNA            98..170
                     /locus_tag="VKU83_00260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:131..133,aa:Gly,seq:tcc)
     gene            202..273
                     /locus_tag="VKU83_00265"
     tRNA            202..273
                     /locus_tag="VKU83_00265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Thr,seq:ggt)
     gene            378..1565
                     /gene="tuf"
                     /locus_tag="VKU83_00270"
     CDS             378..1565
                     /gene="tuf"
                     /locus_tag="VKU83_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLI92005.1"
                     /translation="MSKETFKRDKPHVNVGTIGHVDHGKTTLTAAITTILSSRGLAEA
                     KKYDEIDAAPEEKERGITINTAHVEYATANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTKEHILLARQVGVPRIVVFMNKVDLVDDPELLELVEMEIRELLNT
                     YGFDGDNTPIIQGSATGALAGEAKWVKQIDELMAAVDSYIPLPPRPVDQPFLMSVEDV
                     FSITGRGTVATGRIERGRIKVGEAIEIVGLQDAPLASTVTGVEMFRKLLDQGEAGDNA
                     GLLLRGIEKNQVRRGMVLCKPGSITPHTEFKGEVYVLSKEEGGRHTPFFNKYRPQFYF
                     RTTDVTGEVTLPAGTEMVMPGDNTSLTVHLIAPIAMEKGLKFAIREGGRTVGAGQVTD
                     IIK"
     gene            1649..1719
                     /locus_tag="VKU83_00275"
     tRNA            1649..1719
                     /locus_tag="VKU83_00275"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1681..1683,aa:Trp,seq:cca)
     gene            1738..1935
                     /gene="secE"
                     /locus_tag="VKU83_00280"
     CDS             1738..1935
                     /gene="secE"
                     /locus_tag="VKU83_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLI92006.1"
                     /translation="MNKITLYIRESYRELMEKVTWPSWTELQQQTVITLVATVLITLV
                     VWLMDVVSSTILHFIYSLFGS"
     gene            1957..2559
                     /gene="nusG"
                     /locus_tag="VKU83_00285"
     CDS             1957..2559
                     /gene="nusG"
                     /locus_tag="VKU83_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLI92007.1"
                     /translation="MVETVNQQEVKTQPEGKPAPETKWYVLRVVSGKERKVKEYLDKE
                     ISRGGWGEIVKQVFLPVEKVYKVQNGKKVMRERNYYPGYVMVEVTEGKLGDDLKEVIK
                     NTNSVIHFLGKENPIALRKAEVNKMLGKMDEMAESGSMSMSEPFIIGETIKIIEGPFN
                     DFNGIIEEVNDEKKKLKVTVKIFGRSTPVELNYMQVEKIA"
     gene            complement(2640..2972)
                     /locus_tag="VKU83_00290"
     CDS             complement(2640..2972)
                     /locus_tag="VKU83_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92008.1"
                     /translation="MAKSKKKSAKKAAPKKAAKKAARKKAAPKKAKKAARKAAPKKKA
                     AKKAAPKKAAKKKAAPKKSAPKKAAAKKAAPKKPAPASPAPSMPEPMSSSFGDPTPRF
                     DHDNDNAK"
     gene            3303..3746
                     /gene="rplK"
                     /locus_tag="VKU83_00295"
     CDS             3303..3746
                     /gene="rplK"
                     /locus_tag="VKU83_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLI92009.1"
                     /translation="MAKEISGYVKLQVKGGQANPAPPIGPALGSKGVNIMEFCKQFNA
                     RTQDKMGKTLPVVLTVYSDKSFEFIIKTPPAAVQLLEASKLQSGSKEPNRTKVGKVTW
                     AQVEAIAKDKMPDLNCFTVESAMRMVAGTARSMGITVEGKAPWAN"
     gene            3777..4469
                     /gene="rplA"
                     /locus_tag="VKU83_00300"
     CDS             3777..4469
                     /gene="rplA"
                     /locus_tag="VKU83_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLI92010.1"
                     /translation="MAITKKRKAATAKVDKNKLYTLKEASALVKEVNLTKFDASVDLH
                     VRLGVDPKKADQSIRGTVNLPHGTGKTKRVLVLCTPDKEADARAAGADYVGLDEFIQK
                     IEGGWVDVDVIVATPSVMPKIGRLGKVLGPRNLMPNPKTGTVTNDVAAAVGEVKGGKI
                     AFKVDKAGIIHASIGRVSFTPEKLMENSQELISAIIKAKPATAKGTYVKSVFMASSMS
                     PGIALDAKAFLA"
BASE COUNT         1158 a         1021 c         1321 g         1012 t
ORIGIN      
        1 ccacgagtct catctccgat caccgaagct gatcggggta aagtccggcg cggttgcgaa
       61 gattcgccgc tgagcgcagc gccggagcgg tttttatgcg gaagtagctc agttggtaga
      121 gcgacagcct tccaagctgt aggtcgcggg ttcgaacctc gtcttccgct ccgaagtggc
      181 tgaagtccct ggacttgacc agccgttgta gctcaggggt agagcacttc cttggtaagg
      241 aagaggtcga gagttcaatt ctcttcaacg gctcagaaaa cgaattggag cgattgtata
      301 tagaacaggt ttttagtttc cgatggcggt attaagcctg ttagcatttt aaaacacaac
      361 tttaaaaaca gataacaatg tcaaaagaga cctttaagag ggacaaaccc cacgttaacg
      421 ttggtacgat tggtcacgta gaccatggta agaccacttt gactgccgcg attacgacca
      481 tcttatcttc aagagggttg gcggaagcaa aaaaatacga cgagatcgac gcggcgccgg
      541 aggaaaagga aaggggtatt accatcaata cagcacacgt tgagtatgcg acggctaacc
      601 gtcactatgc gcacgtggac tgcccgggtc acgctgacta tgtaaaaaat atgatcaccg
      661 gggcagccca gatggatggc gcgatcctgg tggtagcggc tacggacggt ccaatgccgc
      721 agaccaagga acacatcctg ctggcgcgcc aggtaggcgt tcccaggatc gtggtattca
      781 tgaataaggt cgacctcgtg gatgaccccg aattgcttga gctcgtcgaa atggaaatcc
      841 gggagttgtt gaacacctac ggcttcgacg gcgacaacac gccgatcatt cagggttccg
      901 ctacgggcgc tcttgccggt gaagcaaaat gggttaagca gatcgacgag ctgatggctg
      961 cggtcgacag ctatatcccg ctgccccccc ggccggtcga tcagccgttc ctgatgtctg
     1021 ttgaggatgt gttctcgatc acgggtcgcg gtacggttgc taccgggcgt atcgaaagag
     1081 gccggatcaa ggtcggcgag gccatcgaga tcgttggcct gcaggatgcg ccgctcgctt
     1141 ccacggtaac cggggtggaa atgttccgca agctgctgga tcagggcgaa gccggagaca
     1201 acgccgggct gttgctccgt ggtattgaaa agaaccaggt ccgtcgcggg atggtgcttt
     1261 gcaaacccgg ttcgatcacc ccgcacaccg agttcaaggg tgaggtatat gtgctgagca
     1321 aggaagaagg tggccgtcat acgccgtttt tcaataagta tcgcccgcag ttctacttcc
     1381 gtacgacgga cgtgaccggt gaggtaacgc taccggccgg cacggaaatg gtgatgcccg
     1441 gggacaatac ctccctgacg gttcacctga ttgcgccgat cgccatggaa aagggcctta
     1501 agttcgccat ccgcgagggc ggcagaacgg taggcgccgg gcaggtgacg gacatcatta
     1561 aatagccgaa aaacggattt tttgatatct ttgtaaggtt aagagctgtt ccgattttgt
     1621 tcgggatagc tcttagcttc tttgatacac gggtatagta taacggtagt acagaggtct
     1681 ccaaaacctt cagtctgggt tcgaatccta gtacccgtgc agtcgaatta ttttaatatg
     1741 aacaaaatca cgctttatat ccgggagtcg taccgcgaat tgatggagaa ggtgacctgg
     1801 ccgtcctgga cggagttgca gcagcaaacg gttatcacgc tggtggcgac ggtattgatt
     1861 acgctggtcg tatggctgat ggacgtcgtc tccagcacga tattgcattt catctattcg
     1921 ttattcggat cctaaccggg tatcaaaatc ttttttatgg tggaaacagt caatcaacaa
     1981 gaagtcaaaa cccagcctga gggcaagccg gcgccggaaa ccaaatggta tgttctgcgg
     2041 gtcgtgagcg ggaaagagcg caaggtgaaa gagtatctgg acaaggagat ctcgcgcggc
     2101 gggtggggag aaattgtaaa gcaggtcttt cttcccgtgg agaaggtcta taaagtgcag
     2161 aacggcaaga aagtgatgcg ggagcgcaac tattatcccg gctatgtaat ggttgaggtg
     2221 accgagggga agctcgggga tgatctcaag gaagtgatca aaaataccaa cagtgtcatt
     2281 cacttcctgg gtaaggaaaa tccgatcgcc ttgcggaagg ccgaagtgaa caagatgctc
     2341 ggcaagatgg atgagatggc ggaatcaggc agcatgagca tgagcgaacc gttcattatc
     2401 ggggaaacga tcaagatcat tgagggaccg tttaacgatt ttaacggcat tatcgaagag
     2461 gtaaacgacg aaaagaagaa gctgaaggtg acggtgaaga tcttcggccg ttcgacgccg
     2521 gtagagctta actatatgca ggtggagaag atcgcatagg cgggctgcgc atcccgcgcc
     2581 aaacaggatt aaaaaaaacc ttccggctgg aaggtttttt tatctgatcg gctattaaat
     2641 catttggcgt tgtcattgtc atgatcgaag cgcggagtcg ggtccccgaa ggaagatgac
     2701 atgggttccg gcattgaagg agccggggaa gcaggagccg gtttcttcgg agcagctttc
     2761 tttgcggcag ccttcttcgg agcggatttc ttcggagcag ccttcttttt agcagctttt
     2821 ttcggggcgg cttttttagc agctttcttt ttaggagcag ccttcctggc agcttttttc
     2881 gcttttttag gggccgcttt cttcctggca gctttttttg ccgctttttt aggggctgct
     2941 tttttggccg actttttctt agactttgcc atagtttgat gagcgtttta agttgttagt
     3001 aagaattgta atggattact aacggaaaag ttagtcagaa aaatccaaat ataaaaaaat
     3061 gacagaaaaa tttcttggca agcaacggtt tggaaggcaa gcgagttcga ggtaacggtt
     3121 aggatgagga ggaatgatcg gtggtgacgg ctgacaaaaa gtccagggaa aaataggcca
     3181 tatcagcctt ttttcattaa ctttgcgtcc ccaaatagaa agttcttttt cacagatggt
     3241 ttgggagttc cagccggttg gtccttaccg ccggggggac gttaacacca aaaacaagta
     3301 acatggcaaa agagatttcc ggttatgtaa agttgcaggt aaagggcggc caagccaacc
     3361 ctgcaccgcc tatcgggccg gcgttaggta gtaagggcgt gaatatcatg gagttctgca
     3421 agcaattcaa tgcgcggacc caggataaaa tggggaagac cctcccggtt gttcttacgg
     3481 tttattccga caaatcgttt gagttcatca ttaaaactcc gcccgcggcg gtccagttat
     3541 tggaagccag caagcttcag agcgggtcaa aggagccaaa tcgtacaaaa gttgggaaag
     3601 tcacctgggc gcaggtggaa gcgatcgcaa aggataaaat gcctgacctg aattgcttca
     3661 ccgtcgaaag tgcaatgcgg atggtggccg gtactgcccg gagcatggga atcacggtcg
     3721 aagggaaagc accctgggcc aattaattag gtgattaact tcaaaatcat tacgaaatgg
     3781 ctattacaaa gaaaagaaaa gctgccaccg caaaggtgga taagaataag ctctatacct
     3841 tgaaggaggc ctccgccctg gtgaaagagg tcaacctgac gaaattcgac gcatccgtgg
     3901 acctgcatgt gcgcctcggg gtagacccga aaaaggccga tcagtcgatc cggggaacgg
     3961 tcaatctgcc gcatggcacc ggtaagacga agcgggtgct tgtgctttgc acgcccgata
     4021 aagaggccga tgccagggca gccggcgcgg attatgtggg cctggatgaa ttcattcaaa
     4081 agatcgaagg gggatgggta gacgtcgatg tcatcgtggc taccccctcg gtaatgccca
     4141 agatcggcag attgggcaag gtgctgggcc cgcgcaacct gatgccaaat cccaagacgg
     4201 gcacggtgac caatgatgtc gcggcggctg tcggagaagt gaaggggggc aagatcgcct
     4261 tcaaagtcga caaggctggg atcatccatg cgtcgatcgg tcgcgtgagt tttactcccg
     4321 agaagctcat ggagaacagc caggagctga tcagcgcaat tatcaaggca aagccggcga
     4381 cagccaaggg tacatacgtg aagagcgtgt tcatggcgag ctcgatgagc ccgggaatcg
     4441 ctttggatgc aaaggcattt ttggcttaac cggcggaaag ccaatcattg ccatcaaacc
     4501 aagatgaatc at
//
LOCUS       DATGKE010000017         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1548596, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000017 DATGKE010000000
VERSION     DATGKE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548596"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="VKU83_00305"
     CDS             <1..2295
                     /locus_tag="VKU83_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HLI92011.1"
                     /translation="EVVGIHQLIETAYQLISRQDSETLRILDNVIILLTHANPDGQEL
                     VSDWYMRNSLPEHRSLDYVPRLWEKYAGHDNNRDFYMLNLKETQNICRELFVDWIPQI
                     MYNHHQAGPAGSILAGPPFRDPFNYVFDPLVMTSLDAVGAAMVSRLNAEGKPGYTERG
                     GSNYSTWYNGGLRTTTYFHNMVGLLTEIIGGPNPSEVPLVPQRLLPNGSTPFPVTPRK
                     WLFRQSIDYSVSLNYAVLDYASRYRDELLFNIYRMGRNSIDRGSEDHWTLSPKMIDRI
                     EELYHSSQASGQSPRGGRSGPATGSGAGSGSGESGIPVRFYDSVLHNPALRDARGYIV
                     PADQPDFPTAVRFIDALIGDGIVVSKATAGFTVNGKLYPAGSYIVKTDQAFRPHVLDL
                     FEPQDHPNDFMYPGGPPVPPYDAAGWTLAYTMGVRFDRILDAFDGPFQRIPFGEWQTV
                     PAQQPGNGWLDARSNAAFVAVNDLLKSGKPVYRSHSTGDFHVSGRQPRHAARIRPARI
                     ALWDTYGGSMPSGWIRWIMEQYHFPYQVIYARDIDSGGLRAKYDVIVLVGGAVPAVDT
                     SAQQGFRGRREPNADSIPAEFRSHLGRLTAGKSVPQLRRFLEGGGHIVTIGSSANLAY
                     QLGLPVHDALTELVGGKERRLPPEKFFIPGSVLRVSLDTAIQPAWGMGPTADVYFDGS
                     PVFNIEPAAYANGSIRPIAWFADDHPLRSGWAWGQAYLRNGVAAFVAGVGGGKLYVFG
                     PEITFRGQTHGTFKLLFNQLYVAE"
     gene            2373..3053
                     /locus_tag="VKU83_00310"
     CDS             2373..3053
                     /locus_tag="VKU83_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLI92012.1"
                     /translation="MPENSEFYPQVMQRYVSDHALPGQYTEFIRTLVRVFDEKLFAAR
                     DFESFPIALILDYVERTHVFYFEKKLPEIEQSILLLSDHYESHHPILAALRNFFNRYV
                     ADLSEHIRAEEEILLPYIKTLLGAGQTAANFSNFVLARRDYSIDRFLEEHHDTEDELK
                     DIRQTIRLYAPPKTNESLYRILLSQLQAFEQDLSVHASIEEEVLIPKARALEKELNAQ
                     LLYIFPFN"
     gene            complement(3077..>5113)
                     /locus_tag="VKU83_00315"
     CDS             complement(3077..>5113)
                     /locus_tag="VKU83_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92013.1"
                     /translation="WADSSFFKIFTFPILKGDADPLAGVDNIVLTRSTAKKYFGDEDP
                     IGKILRVREEKDYRVAAVVADPPANSQLQFDFLARFDNLNKPDEWYTANWITYLLLKD
                     PNSFTTLERQINAYMETPAIRAETGVKGTDYLHFHLEPLARVHLYSALQGFTPNGSIV
                     AIYALTIIAILIMAIACFNYTNLAIAQSTSRMGEIGVRKVLGAAKSQLFAQFTGESVL
                     AAFIALMLAIVLSAQLLPAFDDLSGKHLAVRSLLRPVLLAVILATGLLIGLLAGAYPA
                     LILSSTRLIHILRSGFRLTGGRRGLRQSLVILQFAISLFLIITTMVILQQMDYIRHKD
                     LGFDRDHVLVLPIDRTIHRRYPSIKTVISQLPGVISLSGSYNPPVDVGWEDDVSTNNG
                     KGDVHVLVHAIPTDLNFIGTMDMHLLAGRDFKPEDQPAITGEDSTKPLYRFILNETAA
                     RKIGWTPQEAIGKRLDKNAPGIVVGVVRDFNFASMHEAIGPLVIFPDTQWVHYLLVRV
                     NDTHLPALIARLQAAWKSIVPSQPFSYHFLDDDYNRLYEADQRTGKLFSVFSALAIFL
                     ALLGLFGLAAISTVQRTKEIGIRKVLGADMLNIAFLLARNFLWLIVIAFGVATPLAWI
                     VSSNWLNRFSYRISLPVLTFPLAGFAVLLVAFATVGFHAWRAARTNPAISLRTE"
BASE COUNT         1112 a         1444 c         1490 g         1067 t
ORIGIN      
        1 gaggtcgtcg ggattcacca gctgatcgaa acggcctatc agctgattag ccggcaggac
       61 tccgaaaccc ttcggatcct ggacaacgtc atcatcctgc ttacgcacgc caatccggat
      121 ggacaggagt tggtatccga ctggtacatg cgaaacagtc ttcccgagca ccggtccctc
      181 gactatgtcc cgcgcctgtg ggaaaaatac gccggccatg acaacaaccg ggatttctac
      241 atgctgaacc ttaaggagac gcagaatatc tgccgcgaac tctttgttga ctggatcccg
      301 cagatcatgt acaatcacca ccaggcgggt ccggcaggga gcatcctggc cgggccgccg
      361 ttccgtgacc cgtttaatta tgtttttgat ccgctggtaa tgaccagcct tgacgcggtg
      421 ggtgcggcga tggttagccg gctgaatgcc gaaggcaagc ctggctatac ggaacggggt
      481 gggtcgaact attcaacctg gtacaatggc ggtttgcgaa cgaccaccta ttttcacaat
      541 atggttggcc tgctaaccga gatcatcggc ggccccaacc ccagcgaggt gcccctcgtt
      601 ccccagcggc tgctgccaaa tggctccacg cccttccccg tgacgccgcg taaatggctc
      661 ttccgccagt cgatcgatta ttcggtttcc ctgaattatg ccgtgctcga ttacgcctcc
      721 cgctatcggg acgagttgct tttcaatatc taccggatgg ggcgcaattc gatcgaccgc
      781 gggagcgaag atcattggac gctttcgccg aaaatgatcg accggatcga agaattgtat
      841 cattcttcgc aggcgtccgg ccagtccccg cgcggaggcc gcagcggtcc ggccaccggc
      901 agcggcgccg gctccggctc cggcgaatcc ggaataccgg tgcggtttta tgacagcgtc
      961 ctgcataatc cggcgctgcg cgacgcgcgt ggttatatcg tgcccgccga ccagccggac
     1021 tttcccaccg ctgtgcgctt catcgatgcc ctcatcggcg acggaatcgt cgtatcgaaa
     1081 gcgacggcag gcttcaccgt aaatggcaag ctttatcctg ccggatccta tatcgtaaag
     1141 accgatcagg cgttccggcc ccatgtgctg gaccttttcg agccccagga ccatccgaat
     1201 gatttcatgt atcccggcgg tccgccggtc ccgccgtatg atgcagcggg ctggaccctc
     1261 gcgtacacca tgggcgtccg gttcgaccgg atcctcgatg cgttcgacgg tcccttccag
     1321 cgtatccctt ttggcgaatg gcaaacggtc cctgcgcagc agccggggaa tgggtggctc
     1381 gatgcaagga gcaatgccgc ttttgttgcc gtcaacgacc tgctgaaatc gggtaagcct
     1441 gtctatcgaa gccattcgac cggcgacttc cacgtgtccg gccgccagcc gcgccacgcg
     1501 gcccggatcc ggccggcgcg catcgctctt tgggatacct atggcggttc catgccttcc
     1561 ggctggatca ggtggatcat ggaacaatat catttcccct accaggtcat ttacgcaagg
     1621 gacatcgatt cgggcggtct ccgggcaaaa tatgacgtca tcgtccttgt cggcggcgcc
     1681 gttcctgctg tcgataccag cgctcaacaa ggtttccgcg ggcgtcgtga acccaacgcc
     1741 gacagcatcc cggcggagtt ccggagtcac ctgggccggc ttactgccgg gaagtcggtt
     1801 ccccagttgc ggcgtttcct tgaaggaggc gggcatattg tcactatcgg atccagcgcc
     1861 aacctcgctt atcagctggg tctcccggtc catgacgcac tcaccgaact tgtgggggga
     1921 aaagagcgcc ggctgccgcc ggagaagttc tttattcccg gcagtgtcct tcgggtcagc
     1981 ctcgatacgg ccatccagcc ggcctggggc atgggcccga cggcggacgt ctattttgac
     2041 gggagccctg tcttcaacat cgaacctgca gcctatgcaa acggttcgat ccggccaatt
     2101 gcctggtttg ccgacgacca tccccttcgc agcggttggg cctggggcca ggcttactta
     2161 cggaacgggg tagccgcatt cgtcgccggc gtcggcggtg gcaagctata tgtttttggc
     2221 ccggagataa cattccgcgg ccagacgcat ggaaccttca aactgctttt taatcaactg
     2281 tatgtggccg agtagggcgc aggcgccctc cctgaacgca attttccgga tcgccatatt
     2341 tttgtacctt gtcatgatta aaagcaggaa cgatgcctga aaacagcgag ttttatccac
     2401 aggtaatgca gcgatacgtg tcggatcacg cgttacctgg tcaatatact gagtttatcc
     2461 ggacgctggt ccgggtattc gatgaaaagc tattcgcagc gcgggacttc gaatcgttcc
     2521 cgattgcgct catcctcgat tacgtcgaga gaacccacgt attttatttc gaaaagaaat
     2581 tgccggaaat tgagcagtcc attctgctgc tctccgacca ttacgaaagt caccacccga
     2641 tccttgcggc cctgcgaaat tttttcaacc ggtatgtcgc ggacctctct gagcatatcc
     2701 gggctgaaga ggagattttg ctgccttata taaagacgct tctcggcgcc ggccaaaccg
     2761 ccgcgaattt ttcgaatttt gtgctggcgc ggcgcgacta ttccattgac cggtttctcg
     2821 aggaacatca tgacacggaa gacgagctca aagacatccg ccagacgatc cggctctatg
     2881 cgccgccgaa aaccaacgaa tctctctacc gcatcctgtt aagccagctg caagctttcg
     2941 aacaggacct ttccgtgcac gccagcattg aagaagaggt gctgatcccc aaggcgcgcg
     3001 ccctggagaa ggaattgaat gcgcaattgc tttatatctt tccgtttaac tgaagttttg
     3061 ccggtaccgc tacagctcat tccgtccgca gactgattgc cggattcgtg cgcgcggcac
     3121 gccaggcatg aaagcctacc gttgcaaagg caaccaaaag gacggcgaaa ccggcgaggg
     3181 gaaaggtcaa aacgggcagg ctgatgcgat acgaaaagcg attcagccag ttcgaggaaa
     3241 cgatccatgc caggggcgta gcaacaccga aggcgataac aatcaaccac aaaaaattcc
     3301 gggcgagcag gaaggcgata ttcagcatgt cggcgccaag cacctttcga atgccaatct
     3361 ctttcgttcg ttgcaccgtg ctgatagcgg cgagtccaaa tagcccaagc agagcgagga
     3421 agatcgccag ggctgagaaa acgctaaaca gtttccccgt acgctggtcc gcctcgtaaa
     3481 gccggttata gtcgtcgtcg aggaaatggt agctgaaggg ctgcgaggga acgatgcttt
     3541 tccaggccgc ctgcagccgg gcgataagcg ccggtaaatg cgtgtcattg acgcggacca
     3601 ggaggtagtg tacccactgg gtgtccggga agatgacgag gggcccgata gcctcatgca
     3661 tggatgcgaa attgaaatcg cgaacgacgc caacgacaat gcccggtgcg ttcttatcga
     3721 gccgcttgcc gatggcttcc tgcggtgtcc aaccaatctt gcgggcggcg gtctcattaa
     3781 gaataaaacg gtatagcggc tttgtcgagt cttcgccggt gatcgcgggt tgatcttcgg
     3841 gcttgaagtc cctcccggcc agcaggtgca tgtccatcgt accaatgaag ttgaggtcgg
     3901 ttggaatggc atggaccagg acatgaacat cccccttgcc attgttcgtg ctgacgtcat
     3961 cctcccagcc tacgtcaaca ggtgggttgt agctaccgga aagactgatg accccgggca
     4021 actggctgat gaccgtcttg atggatggat accggcggtg gatcgtgcga tcgatgggca
     4081 aaaccaatac atggtcgcgg tcaaagccaa ggtccttgtg ccggatatag tccatttgct
     4141 gcaggatcac catggtggtg atgatcagga aaagggagat cgcgaattgg aggatgacga
     4201 gtgactgccg caaaccccgg cggccgcctg tcaggcgaaa accggaacga aggatatgga
     4261 tgagccgggt cgaagaaagg atcagggccg ggtaggcgcc cgccagcagg ccaatgagga
     4321 ggcccgtcgc caggataacg gccagcagaa ccggccggag cagcgaccgc acagcaaggt
     4381 gcttgcccga aaggtcatca aaggcaggca gcagttgggc gctgagaacg atcgccagca
     4441 tcagcgcaat aaatgccgcg aggacggact cgccggtgaa ttgagcgaac aattggcttt
     4501 tcgcggcgcc gaggaccttg cggacgccga tctcgcccat gcgggaagtg gattgggcaa
     4561 tcgccaggtt ggtatagtta aagcaggcta tcgccatgat caggatagca atgatggtca
     4621 gcgcatagat tgccacgatg ctgccattcg gggtaaagcc ctgcaaagcg gaataaagat
     4681 ggacacgggc gaggggctcc agatgaaaat gcagatagtc ggttcccttc acacccgttt
     4741 ctgcacggat ggctggtgtc tccatatagg cattgatctg cctctcgagt gtcgtgaatg
     4801 agttcggatc tttgagcagg agataggtaa tccaattggc agtgtaccat tcatcgggtt
     4861 tgttgaggtt gtcgaagcgg gcgagaaaat cgaattgcag ctgcgagtta gcgggcggat
     4921 cggcaacgac cgccgccacc cggtagtcct tttcctcccg gacccgaagg atcttgccaa
     4981 tggggtcctc atcgccaaaa tactttttcg cggtcgagcg ggtaagaacg atattgtcta
     5041 cgccggccag cggatcggca tcgcccttaa ggatcgggaa ggtgaagatc ttgaagaacg
     5101 aggagtcagc cca
//
LOCUS       DATGKE010000018         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_826855, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000018 DATGKE010000000
VERSION     DATGKE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826855"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VKU83_00320"
     CDS             complement(<1..1678)
                     /locus_tag="VKU83_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HLI92014.1"
                     /translation="MSKWRPAPVFLLIALNAMLLFFVILEDRLAIPRWLQVAGRLHPM
                     ILHFPIVLVVAYAILLWFKPIPFAGEVLMLSAFAAVITALAGVFLATESTHEGSAIQW
                     HKWLGTLTSVSLLTLYLLKDSSLITRSRQKWLAALPLVAVFIAGHYGGDLTHGANYVL
                     APLRPATPAAPPLDEANAYHDVVYPILDSRCIGCHNSADSKGGLNMEDPLQFARGGRD
                     GRPWDTTADGFGLLMTRLHLPADDKKHMPPINTTQLSQQQLDILSAWVRDGSPFDKKV
                     LDLPATDTLRLIASRLLRSGEDEDFDFPAADEKTIAALNTNYRTVIPLASGSPALAVD
                     FYGASVFQPKQLQELSRIKDQIVWLNLDKMPVADNDLSSITTFRNLRRLNLDFTSITG
                     SVLAMLSRLSHLQSLSLSGDKIKSGDLDQLTRIRTLHELYIWQTGLPQDAVARLRQKR
                     KDLDVNTGFNADTVRIKLNPPRLETEARIIRDSGVAIKMRHFVPGAVIRYSLDGSDPD
                     SGGRVYTGPFVLKDRSLFRAQAYRPGWLPSDPVLATFYTQKFLPDSIRLLL"
     gene            complement(1715..>2505)
                     /locus_tag="VKU83_00325"
     CDS             complement(1715..>2505)
                     /locus_tag="VKU83_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92015.1"
                     /translation="ILIFDTNGRLMDNWGHEFPGGHGLTLHDENGTEFLYITDIVRNQ
                     VFKTTIDGQILLTINAPLDAGIYKASKEFVPTETAIDSNGDIFIADGYGAQYILHYDR
                     TGKLVNYFGGRGDGDAHFDNAHGICWDTRRRGRTLLITDRSRNCFKRFTREGKLLEVI
                     SLPGACVCRPVIHRDHLYAAVLRSPDLNAVGTGFVTILDKHNKVVSNIGGSEPTYKNG
                     LLQPMRQTEKIFVHPHDVCVDRDENLYVAQWASGKVYPYKLNRV"
BASE COUNT          547 a          636 c          800 g          522 t
ORIGIN      
        1 gcagcagcag gcgaatcgag tcggggagaa atttctgggt gtaaaacgta gccaaaacgg
       61 gatcgcttgg gagccagccg ggccggtaag cctgtgcgcg aaacaggctg cgatctttga
      121 gcacaaacgg tccggtgtaa acgcggccgc cgctgtcggg gtccgagcca tcaagggaat
      181 aacgaatgac cgcgcccgga acgaaatggc gcattttaat ggcgacgccg gaatctcgga
      241 taatccgggc ctcggtctca agccgaggcg ggttgagttt gatccgtacc gtgtcggcat
      301 taaaacccgt attcacgtcg agatctttcc gtttctgccg gagcctggcg actgcgtcct
      361 gcggcaggcc ggtttgccat atataaagtt catgcagcgt cctgatcctg gtgagctgat
      421 cgaggtcacc cgatttgatc ttgtcgcccg aaaggctgag gctttggaga tgggaaagcc
      481 gggaaagcat agcgaggacg gagccggtga tgcttgtgaa gtcaaggttg aggcggcgca
      541 agttgcggaa agtcgtgata ctgctaaggt cgttgtcggc gacgggcatc ttatcaaggt
      601 ttagccagac gatctggtcc ttgatgcggg ataactcctg caactgttta ggctggaaaa
      661 ccgatgcacc gtagaagtcg acggccaggg ccggagagcc gctggcaagc gggataaccg
      721 tccggtagtt ggtattgagg gccgcaatcg tcttttcgtc tgcggccggg aagtcgaaat
      781 cttcatcttc acccgaccgg agaagcctgg atgcgataag tcgcagggtg tcggtggcgg
      841 ggagatcgag cacttttttg tcaaacgggg agccgtccct cacccaggcg gaaagaatat
      901 cgagctgctg ctggctcagt tgcgtcgtgt tgataggagg catatgtttc ttgtcgtcgg
      961 ccggaagatg gagccgggtc atgaggagcc cgaaaccatc ggcggtcgtg tcccagggcc
     1021 tgccatcccg accgcccctt gcgaattgca gcggatcctc catattgaga ccgcccttgg
     1081 aatcggcgga attatggcag ccgatgcatc gcgaatccag gatcggatag acgacgtcgt
     1141 ggtaggcgtt cgcctcgtcc aacggcgggg cggccggagt cgcgggcctt aggggcgcca
     1201 ggacgtaatt ggcgccatgg gtcaggtcac cgccgtagtg gccggcgatg aaaacggcga
     1261 cgaggggcag ggcggccagc catttttgcc gggatctggt gatcagcgaa ctgtctttta
     1321 ggagataaag cgtcaggagg gacacagagg tcagggtccc cagccattta tgccactgga
     1381 tggccgaacc ctcgtgcgtg gattcggttg ccaggaacac gccggccagc gccgtgatca
     1441 ccgccgcgaa ggcggacaac atgaggactt cgccggcaaa agggattggc ttaaaccaaa
     1501 ggaggatcgc ataggcgacg acgagaacga tgggaaaatg cagtatcatc gggtgtaatc
     1561 gtccggcgac ctgcagccat cgggggatgg cgaggcggtc ctccaggatc acgaagaaaa
     1621 gcagcatcgc gttgagcgcg atcagcaaga ataccggggc gggtctccat ttactcattg
     1681 gggcaaggtg ggtgaggctg caaaaaagtg gcgatcacac cctgtttagt ttataagggt
     1741 aaacttttcc ggatgcccat tgggcgacgt acaggttttc atcgcggtct acgcagacat
     1801 catgcggatg aacgaagatc ttttcggtct gacgcatcgg ctgaagcagg ccgtttttgt
     1861 acgttggctc gctgccgccg atattgctga ccaccttatt atgtttgtcg aggatggtca
     1921 caaagcctgt gcccacggca ttgaggtccg gtgaccgcag aaccgctgcg tacaggtgat
     1981 cacggtgaat gacgggcctg cacacgcaag cgcccggaag ggaaataact tcaaggagtt
     2041 taccctccct ggtgaaccgc ttaaagcaat tgcgactcct gtcggtgata agcaaggttc
     2101 ggccccggcg ccgggtgtcc cagcagatgc cgtgggcatt gtcaaaatgc gcgtcgccgt
     2161 cgccacggcc gccgaaatag tttacgagct ttcccgtcct gtcataatga aggatatact
     2221 gcgcgccgta gccgtctgca ataaaaatat ctccattgct gtcgatagcc gtttccgtcg
     2281 gaacgaactc tttggacgcc ttatagatgc cggcgtccaa tggcgcgttt atggtaagaa
     2341 gaatctggcc gtcaatggtg gttttgaaaa cctgattgcg aacgatgtcg gttatgtaaa
     2401 ggaattccgt accgttctca tcgtgaaggg taagcccgtg gccgcctggg aactcgtgtc
     2461 cccagttgtc catcaaccgg ccattggtat cgaagatgag gatgt
//
LOCUS       DATGKE010000019         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_827853, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000019 DATGKE010000000
VERSION     DATGKE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827853"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VKU83_00330"
     CDS             <1..258
                     /locus_tag="VKU83_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92016.1"
                     /translation="DDATKSIAIIVNYVTAAIAEGLAERQAAKEEDVEDASADENERK
                     AARLQAEAENEASRSRGEKVRGTAQPKRRIPNSGPRRTGAR"
     gene            353..1201
                     /gene="tsf"
                     /locus_tag="VKU83_00335"
     CDS             353..1201
                     /gene="tsf"
                     /locus_tag="VKU83_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLI92017.1"
                     /translation="MSTATITAAEINKLRQTTGAGMMDCRKALVESDGDFEKAIDYLR
                     KKGQKVAALRNDREAKEGVIIATTTPDHKTGYIVTLACETDFVAKNADFVAFAQSIAN
                     VALKNNVRSLEELNASALDGATVADKINDQVARIGEKIQLARFERIEAEAVAAYIHGA
                     YRMGVLVGLSKNSPALLEAGKDIAMQIAAMNPVAVDPDSVPAEIVSREKAIVTEQIKN
                     DPKMGGKSDEMIDKIAVGKLNAFFKENTLTAQAFVKDASKTVGDYLKSVDSSAKVTQF
                     KRVQLG"
     gene            1280..1993
                     /gene="pyrH"
                     /locus_tag="VKU83_00340"
     CDS             1280..1993
                     /gene="pyrH"
                     /locus_tag="VKU83_00340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLI92018.1"
                     /translation="MVPKYKRVLLKLSGEALMGDKSFGFDNEVIAQYAQDIKMIVELG
                     VQVAIVIGGGNIYRGMNEAETGIERAHGDYMGMLATVINGMAMQAGLEKIGVYTRLQS
                     AIKMEQIAEPYIRRRAIRHVEKGRVVIFGAGTGNPYFTTDTAGSLRAIEINAEVILKG
                     TRVDGIYDADPEKVPSAKKFEVITFQDCISKNLRVMDMTAFTLCMENHLPIIVFDMNK
                     PGNLLRVVRGEKVGTLVRD"
     gene            2230..>2448
                     /locus_tag="VKU83_00345"
     CDS             2230..>2448
                     /locus_tag="VKU83_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92019.1"
                     /translation="MNSLHTVEMMILGFQLTVGLPEVIICELGALILGFTIHYFWTSK
                     KDLTIDDAAPAISDNDNWKLKYYNDMDMQ"
BASE COUNT          646 a          665 c          653 g          484 t
ORIGIN      
        1 gacgacgcca ccaaatcgat cgccatcatt gtcaactatg ttacggctgc gattgccgaa
       61 gggcttgccg aaagacaagc tgcgaaggaa gaggacgtcg aggatgcatc ggcggatgag
      121 aacgagcgga aagccgcccg cctccaggcc gaagccgaga acgaagcttc ccgcagccgc
      181 ggagaaaagg taaggggtac cgcccagcca aaacgccgga tcccgaactc gggcccgcgc
      241 cgcaccggag cgcggtaaga cgggtccgat gggcctgccg gccgccgaag gcggccggat
      301 atcagatttc aggtgggccg aaggcccatc cacgtataat aaactcaatc atatgtcaac
      361 tgcaacaatc accgcagcag agatcaacaa actgcgccag acgaccggcg cggggatgat
      421 ggattgccgc aaggcactgg tggaaagcga tggcgacttt gaaaaagcca tcgactacct
      481 gcgcaagaaa ggtcaaaagg tcgccgcact gcggaacgat cgcgaagcga aggaaggagt
      541 gattatcgct acaacgactc cagaccacaa gaccggctat atcgttaccc ttgcttgtga
      601 gacggatttt gtggccaaga acgcagactt cgttgccttt gcccaaagca tcgctaacgt
      661 cgcgttgaag aataacgtcc ggtctctcga agagctgaac gcttccgccc tcgacggcgc
      721 caccgtcgcc gacaagatca acgaccaggt cgcccggatc ggagaaaaga ttcagcttgc
      781 gcggttcgaa cggatcgagg cagaagccgt tgccgcctat atccatggcg cttaccgcat
      841 gggcgtcctg gtggggctgt cgaagaactc tcctgccctg ctggaagcag gcaaggacat
      901 cgcgatgcag atcgcagcca tgaaccctgt ggccgtcgac ccggactcgg ttcccgccga
      961 gattgtgagc cgcgagaagg cgatcgtcac cgaacaaatc aagaacgatc ccaagatggg
     1021 gggcaaatcc gatgagatga tcgacaagat cgcggtcggc aagctcaatg cctttttcaa
     1081 ggagaacacc ctcactgcgc aggctttcgt caaggatgcg tccaagacgg taggggacta
     1141 tctgaaaagc gtcgacagct ccgcaaaggt gacccaattc aagagagtcc agctcggata
     1201 acaaaataaa aaaacaggtg cttgtcgcct gttttttttt acttttacct cacgcagcct
     1261 tcacccaacc ttcacactca tggtgcccaa gtacaaacgc gttcttctca aactttccgg
     1321 cgaggccctc atgggcgata agagtttcgg ctttgacaat gaggtgatcg ctcaatacgc
     1381 tcaggacata aagatgatcg tggagctggg cgtgcaggta gccatcgtca tcggcggcgg
     1441 aaacatctac cgcggaatga atgaagcgga aacgggaatc gaacgagcgc acggcgacta
     1501 tatgggaatg ctcgccaccg tcatcaacgg gatggccatg caggccgggc tggaaaagat
     1561 cggcgtctat acccggctgc aaagcgccat taaaatggaa cagattgccg aaccctatat
     1621 ccgtcgccgg gccattcgac atgtcgaaaa aggtcgcgtc gtgatcttcg gcgccggtac
     1681 cggaaacccg tatttcacca ccgataccgc cgggtctctc cgggcaatcg aaatcaacgc
     1741 cgaggtcatc ctgaagggca cccgcgtgga tggcatttac gacgccgacc ctgaaaaggt
     1801 gccttccgcc aagaagtttg aggtcataac attccaggac tgcatctcca aaaatctccg
     1861 ggtcatggat atgaccgcct ttacgctttg catggagaat cacctcccca tcatcgtgtt
     1921 cgatatgaac aagccgggca acctgctccg ggttgtcaga ggggaaaaag ttggcacgtt
     1981 ggtaagagat tgattacaag cagattacaa aaattgatcg aagaaacccg ctgataggat
     2041 ttgcatttat gcagcaggaa tagtaatttg tgcaatctta tatagctacg ccacctcgca
     2101 agtttttttg ccttccctcg gtctatattc ccttgaaaaa cggtttgttg tttatcggcg
     2161 caatccctaa acgcaatatt gcatgtataa aaactagagt ttggtaaaat ctttttgttc
     2221 cgaaccatta tgaacagctt acataccgtg gaaatgatga tcctgggatt tcagctgact
     2281 gtcggtctgc ctgaagtgat catctgcgaa ctcggcgcgc tcatcctcgg attcaccatc
     2341 cactatttct ggacgtcaaa aaaggacctc accattgacg atgccgcccc ggccatcagc
     2401 gacaacgata actggaaact caagtattac aacgacatgg atatgcag
//
LOCUS       DATGKE010000020         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1137085, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000020 DATGKE010000000
VERSION     DATGKE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137085"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VKU83_00350"
     CDS             complement(<1..1227)
                     /locus_tag="VKU83_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HLI92020.1"
                     /translation="MRCPLLAKFLLPLFSAAVAAATPAAAQNPQLSAVVNYALNRPGV
                     VMIRTEYTANVYVNSMRFDDKAFNQLLDSIQRLDHGGGVMPEQKLDIVLREMNSRPER
                     FFQTTFDYIKQPEQITATGTGFFISSDGFVATNCHLIERDDNFIRRSFILTAFRQITD
                     ASIAALETSWATRFNEQQRSLLYNTYASVYSRLFSMILYDLKKDIYVVYRRDSAGMHS
                     DTVKKAAIVVIKGQPMPGKDVAILKVRVPANMPMLTLASAELPQVGEQLYVYGYPAPV
                     TKNDFVSAESAIEPTLTTGIISAIKKSVGGWPLIQMDANINHGSSGGPVCDEKGEVVG
                     LTTFGSLETNGGLAAGLNFAVPVSILDEYIDSAGINIQPTPVTGLFARALIDYEQRQY
                     HNALRNFEKVYRLNSQY"
     gene            1297..>2018
                     /locus_tag="VKU83_00355"
     CDS             1297..>2018
                     /locus_tag="VKU83_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI92021.1"
                     /translation="MDSSQAPLAERLRPQTLDQLVGQQHLTGKNSVLRKAIEHGKPPS
                     MILWGPPGTGKTTIAGIIAHTLQVPFYTLSAISAGVKEVREVIAEAREKTRAILFIDE
                     IHRFNKGQQDALLGAVEKGIVTLIGATTENPSFEVNSALLSRCQVYVLKPLTEEDLLV
                     VLDHAFRDDAWLRGLHIDLKETTALLTISGGDARKLLNLLEVVADALGKEGVITDEAV
                     MQIAQQRIALYDKKGEQHYDIIS"
BASE COUNT          433 a          560 c          603 g          422 t
ORIGIN      
        1 atattggctg ttcagccgat acaccttttc gaagttgcgg agggcattat ggtattggcg
       61 ctgctcataa tcgatcagcg cccgggcgaa aaggccggtc accggcgtgg gctggatatt
      121 gatgccggcg ctgtcaatgt attcgtccag aatgctgacg ggaaccgcga agttcaggcc
      181 cgccgccagt ccgccattgg tctccaggct tccgaaagtc gtcaggccga cgacttcccc
      241 tttttcgtcg catacgggcc cgccgctgct gccgtggtta atattggcat ccatctggat
      301 caggggccag ccgccgacgg attttttgat ggcgctgatg atcccggtgg taagcgtagg
      361 ttcgatcgcc gattccgccg aaacaaaatc attcttggtg acgggcgccg ggtagccata
      421 gacatacagc tgctccccga cctgcggcaa ttcggcgctc gcaagggtca gcatcggcat
      481 gttggcgggg acccggacct tcagaatggc aacgtccttt cccggcatcg gctgaccttt
      541 gatcacgacg atggcggcct ttttcaccgt gtccgaatgc atgccggcgc tgtcccttcg
      601 gtagacaaca taaatatcct tcttcaggtc gtacaggatc atggaaaaga gccgggagta
      661 aacggaagca tacgtattgt ataacaagct ccgctgctgc tcattgaagc gggtagccca
      721 ggacgtctcc agggcggcga tactggcgtc ggtgatctgg cggaaagccg tcaggataaa
      781 ggaacgccgg ataaaattgt cgtcgcgttc gatcaggtga cagttggtag ccacgaaccc
      841 gtcgctgctg ataaagaaac cggtgccggt ggcggtgatc tgttccggct gtttgatata
      901 atcgaaagtc gtctggaaaa aacgctccgg cctgctgttc atttcccgaa gcacgatgtc
      961 gagcttctgc tcgggcatga caccgccgcc gtggtcgagg cgctggatcg agtccaggag
     1021 ctggttgaag gctttgtcgt cgaaccgcat gctgttgaca tagacattcg ctgtgtattc
     1081 cgtgcggatc atgacgacgc cgggtcggtt cagcgcataa ttgacgacag ccgatagctg
     1141 cgggttctgg gctgcggccg gcgtagcagc ggccactgcc gcggagaaga gcgggagcag
     1201 gaatttagcc aaaaggggac atctcatgct ttgccggttg actttaaaac actaaaaagg
     1261 ataagttgtt gtactattgt cacttaatta tttgttatgg acagttccca ggcgccgctg
     1321 gcagagcgac tgaggcctca gaccctcgat cagctggtcg gtcagcagca tttgacgggt
     1381 aaaaatagcg ttttacgcaa ggccatcgag catgggaagc cgccttcgat gatcctttgg
     1441 ggtcccccgg ggacgggaaa gacgacgatc gccggcatca tcgcccacac cttacaggta
     1501 ccgttctaca cgctgagcgc gatctctgcc ggcgtcaagg aagtccgcga ggtgatcgcc
     1561 gaggcccgcg aaaagacaag ggccatcctc ttcatcgatg agatccaccg gttcaacaag
     1621 gggcagcagg atgccctgct cggcgcggtg gaaaagggca tcgtcaccct gatcggcgca
     1681 acgaccgaaa acccatcctt cgaggtcaac agcgcccttt taagtcgctg ccaggtctat
     1741 gttctgaagc cgctgaccga ggaggacctc cttgtcgtgc tcgaccacgc gttccgcgac
     1801 gacgcctggc tgcgcgggct ccatatcgac ctgaaggaaa cgacggccct gcttacgatc
     1861 tccggcggcg atgcccgcaa actgctcaac ctgctggagg tggtagcgga tgcgctcggc
     1921 aaagaaggag tcatcacgga tgaggccgtc atgcagatcg cgcagcaacg catcgccctc
     1981 tatgataaaa agggggaaca gcattacgac atcatctc
//
LOCUS       DATGKE010000021         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1136866, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000021 DATGKE010000000
VERSION     DATGKE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136866"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VKU83_00360"
     CDS             <1..664
                     /locus_tag="VKU83_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLI92022.1"
                     /translation="VLWWDTPTGMTDDYAEKLHAELALQPRIITNDRLKRPDYPGDYK
                     TPEQKIPKPGELTGDWETCMTMNGTWGFKKSDHNWKTPTTLIHNLVDIASKGGNYLLN
                     VGPTPEGEIPQPSIDALKTIGAWMKVNGEAIYGTKGSPMGPLLWGRCTQKGSTLYLTV
                     FDWPADGRLVVPGVSGEVKRASLLAGGVKLETEKGVDGVVIHVPATAPDAMATVIKLE
                     LN"
     gene            816..>3082
                     /locus_tag="VKU83_00365"
     CDS             816..>3082
                     /locus_tag="VKU83_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLI92023.1"
                     /translation="MKKALLHVVALLLMATVHAQTRVTGYVTDAKGNPLHGVTIAVKG
                     TANGTVSDSSGRFDIRVPGPQTTLVFSLVSYETKEEPVGKRQKMTVILAESGNSMNDV
                     VVIGYGTQKKRDLTGAITTITSKQLEERHTSSLGDALQGMAAGVLVTTAAEPGAQPTI
                     QIRGYSTFSSSGNTPLYVIDGILSDNMSWVNPNDVQSVEILKDAASAAIYGSRSANGV
                     IIVTTKRGIEGKPRIGVQYLHVSGVLAHKLPQANSKEVRLYRNEQGSSGVSTDSLNPS
                     YNADNDYQSVLTRVANRDQVDFSASGAGKGLNFYTSIRAVDDQGLIYNSYAKTLFFRT
                     NVEFSPNSKFRYFGRMQFGYTTGNVIDEGNTIFQAFQRPANYALYNPDGSLTGYISGR
                     RNPLTVVLDQVNLNQNYNGSLFNQIDYTFIPELRLTANAVATYTEIRNQQFLPKILSS
                     NNPLTNNGSESFSRIIDWQSQAYLNYMKTFGGAHAVSLMAGTSAEASQTNGANEAAVN
                     YVSEQVLTLNSGTIIPNGTTTTATADSRASLFGRATYGYKGRYLFATTIRRDGSSRFG
                     PDNRWGDFFSASAAWRFSDERFLRWSNSFLDDGKLRLSYGQTGNESIGDYTAQTTYGF
                     GTYYYNGISGIAPSTTLGNPTLQWEATTQKDLGLDLSFLRSRLLVVADVYEKITTNLL
                     TQSPLPAETGFSNYEVNYGSIRNRGFELSVTGRPVLRRNFDWLVIYNLSVQRGIVTRL
                     NAGLPAIQGNSADAAG"
BASE COUNT          738 a          838 c          908 g          598 t
ORIGIN      
        1 cgtattgtgg tgggacacgc caacgggcat gaccgacgac tatgcagaaa agctgcatgc
       61 cgaactggcg ttgcagccca ggatcatcac taacgaccgg ctcaaacggc cggattatcc
      121 cggcgattat aagacacccg aacaaaagat ccccaaaccg ggggagctga cgggtgactg
      181 ggagacctgt atgaccatga atggcacctg gggtttcaaa aaatcggatc acaactggaa
      241 aacgcctacg acgcttatcc ataatctggt ggacatcgcg tccaaaggcg gtaactatct
      301 actcaatgta gggccgacgc cggaaggcga gatcccccag ccgagcatcg acgcactgaa
      361 aaccatcggc gcctggatga aggtaaatgg agaagccatc tatggaacga agggcagccc
      421 gatgggcccg ctgctgtggg gccgctgcac gcagaaggga tcgacgctgt atcttaccgt
      481 cttcgactgg ccggcggacg ggcggctggt tgtacccgga gtatcgggtg aagtgaagcg
      541 ggccagtctg cttgcggggg gagtgaagct ggagacggaa aaaggggtcg atggggtggt
      601 cattcatgta ccggcaaccg cgcccgatgc gatggcaacg gtcattaagt tggagttgaa
      661 ttaaagcaat aaagcaagag ttagttttgg ctggcgcccc tttacttcgg gggcgtcttt
      721 ttttgccaaa atagtttagt agatggcaat ttgggcgaat tcgggattgc taaaatagga
      781 gcaagttgca gtagaaacca aaactggctt tccatatgaa aaaagcgctc ttgcatgtgg
      841 tggcgctgtt gctgatggcc actgtacatg cgcaaacccg ggtcacgggg tatgtgaccg
      901 atgcaaaagg caatcccctg catggcgtaa ccatcgctgt aaaggggacg gccaacggaa
      961 cggtctcgga cagcagcggc cgtttcgaca tccgggtacc gggaccgcaa accactttgg
     1021 tcttctcgct ggtcagctat gagaccaaag aggagccggt gggcaagcgg cagaaaatga
     1081 ccgtcattct tgccgagtcg gggaacagca tgaacgacgt ggtcgtgatc ggctacggaa
     1141 cgcagaaaaa acgcgacctg accggcgcca tcaccaccat cacctccaag cagctggaag
     1201 aaaggcatac gagcagcctg ggtgatgcgt tgcagggcat ggctgccggc gtgctggtga
     1261 caacggccgc ggaaccgggg gcgcagccga cgatccagat caggggctat tcgacgttca
     1321 gcagctcggg caacacgccg ctctacgtga tcgacgggat acttagcgac aacatgagct
     1381 gggtgaaccc gaacgacgta caaagcgtgg agatcctgaa agacgcggca tcggcggcga
     1441 tatacggttc ccggtccgcc aacggcgtga tcatcgtcac gacaaaacgg ggcattgagg
     1501 gcaagccccg gatcggcgtt caatatctgc acgtgagcgg tgtgctggcc cacaagctac
     1561 cgcaggccaa ttccaaggaa gtgcggctat accgcaacga acaggggtct tccggcgtca
     1621 gcaccgactc gctgaacccc agttacaacg cggataatga ctatcaaagc gtgttgacgc
     1681 gggttgccaa ccgtgaccag gtcgatttca gcgccagcgg cgccggaaag ggtctgaact
     1741 tctataccag catccgggcg gtagacgacc aggggctcat ctataacagc tatgccaaga
     1801 cgcttttctt tcggaccaat gtggagttca gtcctaattc caaattccgg tattttggca
     1861 ggatgcagtt cggctatacc accggaaatg tgatcgatga agggaatacg atctttcagg
     1921 cttttcagcg gcccgccaac tacgcgttgt ataatccgga tgggtcgctt accgggtaca
     1981 tcagcggccg gcggaatccg ctcaccgtgg tgctggacca ggtcaatctc aatcaaaatt
     2041 acaacggatc gctattcaat cagatcgatt atacctttat tcccgagcta aggctgaccg
     2101 ccaatgccgt tgctacctat accgagatca ggaaccagca attcctaccc aagatcctca
     2161 gctcgaataa cccgcttacc aataacggca gcgagagttt ctcccggatc atcgactggc
     2221 agagccaggc ctatctcaac tacatgaaga cctttggcgg cgcgcatgcg gtgagtctca
     2281 tggcgggtac cagcgcagaa gcatcccaga ccaacggcgc caatgaggcc gccgtgaact
     2341 atgtaagcga gcaggtcctg acgctgaatt ccggcaccat tatccctaac ggaactacga
     2401 ccaccgccac tgccgactcg cgggcttcgc tttttggccg ggctacctat ggctataagg
     2461 gacggtatct gttcgccact acgatccgcc gggacggctc ttcccgtttc ggacccgata
     2521 accgctgggg cgatttcttc tctgcttcgg ccgcctggcg gttcagcgac gaacggttcc
     2581 tgcgctggtc gaacagtttc ctggacgacg gcaagctgcg gttaagctat gggcagaccg
     2641 gcaatgaaag catcggggac tatacggcgc agacgaccta tggtttcggg acctattact
     2701 acaatgggat atcgggcatc gcgccgagca cgacgctggg caatcccacg ctgcaatggg
     2761 aggctaccac ccagaaggac ctcgggctcg atctgagctt tttacgatcg agactgctgg
     2821 tcgtcgcgga tgtgtatgaa aagataacga ccaatcttct cacgcaaagc ccgttaccgg
     2881 cggagaccgg tttcagcaat tacgaggtca actacggcag catccggaac aggggatttg
     2941 aattatcggt gacgggaagg cctgttttgc gacggaattt cgactggctg gtcatctata
     3001 acctatcggt ccagcgaggc atcgtcaccc ggctcaatgc cggattgccg gccattcagg
     3061 ggaattcggc cgacgccgct gg
//
LOCUS       DATGKE010000022         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1547583, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000022 DATGKE010000000
VERSION     DATGKE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547583"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VKU83_00370"
     CDS             complement(<1..2207)
                     /locus_tag="VKU83_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92024.1"
                     /translation="MISTEQVYRFLLSVLFYSIPSLLFAQAPHTVSGTVRDLATGETL
                     IGASVLFPDHPRDAVLSNAYGFYSIRAAAGKYQMVVSFTGYRSDTIRLLLQRDTVINV
                     NLAAGARQLQEVVVTANRNANNVSRPLMGVQKLSITEIKDVPVIFGEKDILKTIQLLP
                     GVQSGGDGSSGFYVRGGSVDQNLILLDEATVYNPSHLLGFFSTFNSDAIKDVTLYKGA
                     IPAEFGGRLASVLDVRMNDGNVNDYVVSGGVGLISSRLNVEGPIANGGGSFIVSARRT
                     YADLFLKLSKDTNTSRSRLYFYDLNAKANYRLGPNDHIYLSGYFGKDDLGLGNAFGLD
                     YGNSTATFRWNHILNSRLFSNTSLIYSKYYYDVAINSGNNNIGVTSFIRDYHLKEDME
                     YYANAANKVDFGVDLNNHATSPGVITASATSSFNPLNLPSKYSLESAAYINHDLSVSN
                     RVTLNYGLRASLFTVYGPGNFYRYDSAGNAVDTATYKSGQAVKTYFNLEPRFSMSWKL
                     TANSSVKLAYTRTTQNLHLLSNSTSSNPTDVWIPSSNNVKPELADQVTAGYFRNLREN
                     RYELSVEGYYRDMRNQIDYKNGAQLIANENVESELIFGRGRSYGMELFFKKRVGQLTG
                     WVSYTLSRTERRFAGVNNDTWYPANQDRTHNLAVVGVYRLNRKWTLSGDFVYYTGNAV
                     TWPSGKYMANGEIIFLYTQRNGYRMPPYNRLDLSATLQGKKTKKFESNWNFSL"
     gene            complement(2255..3013)
                     /locus_tag="VKU83_00375"
     CDS             complement(2255..3013)
                     /locus_tag="VKU83_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLI92025.1"
                     /translation="MKSGLVGFLLLLGGIASSAQRISPDTIALSGGALIVQPIVHASV
                     VLTLAGKTIYADPVGGAAKYEGLAAPDIILVTDIHGDHFDPATLRAIRREGTVLVVPQ
                     AVADKMPAAEKTGIVILGNGENKEILGIGVAAIPMYNLPASPTDPRHPKGRGNGYVLS
                     ARGNKLYLSGDTQGIPEMRALTGIDVAFVCMNLPYTMDIKEAAGAVLDFKPRIVYPYH
                     YRGQGGFSDTEGFSKLVKAGDPDIDVRLRNWYPG"
BASE COUNT          688 a          871 c          889 g          710 t
ORIGIN      
        1 taaagcgaaa aattccagtt gctttcaaat ttttttgtct tttttccctg cagcgtggcg
       61 ctaagatcaa gccggttgta cggcggcatc cggtacccgt tacgctgggt gtacaggaaa
      121 atgatctcgc cgtttgccat gtatttcccg ctcggccagg taacggcgtt gcccgtataa
      181 taaacgaaat cgcccgacag cgtccatttt ctattgagcc ggtagacgcc gaccaccgcc
      241 aggttatgcg tccggtcctg gttggcggga taccaggtgt cgttattcac gcccgcaaag
      301 cgacgttccg tcctcgacaa ggtatagctg acccagcccg tgagctgtcc gacgcgtttc
      361 ttgaagaaga gttccatccc gtatgacctc ccgcggccaa agatcagctc cgactccacg
      421 ttctcattgg cgatcagttg tgcgccgttc ttatagtcga tctggttccg catgtcccgg
      481 tagtagcctt cgaccgacaa ttcatagcgg ttctccctga ggttccggaa atagccggcc
      541 gtgacctggt cagcgagctc cggtttgacg ttgttgctgc tcgggatcca gacatccgtc
      601 ggattggaag aggtagaatt cgagagcagg tggaggtttt gcgtcgttct cgtgtaagcc
      661 agcttcacag agctgttggc ggtgagcttc cagctcatgg aaaaacgggg ctcaaggttg
      721 aaataggttt tcactgcctg gcctgacttg taggtggcgg tatcgacagc gttgcccgcg
      781 ctgtcataac gatagaagtt tccgggaccg tatacggtga acaggctggc gcggaggcca
      841 tagttgaggg tcacccggtt cgacaccgac aggtcatgat taatgtaagc cgcactctcc
      901 agggaatatt tgcttggcag gttcagcggg ttgaagctgg acgtcgccga agccgtgatc
      961 accccgggag acgtcgcatg attgttgaga tcgacgccga aatccacttt gttggcagcg
     1021 ttcgcgtaat actccatgtc ctccttgaga tggtagtccc gtataaagga ggttactcca
     1081 atgttattat ttccggagtt gatcgccacg tcataataat atttactgta gatgagcgag
     1141 gtattcgaaa agaggcgact gttcaggata tggttccagc ggaaggtcgc cgtcgaattg
     1201 ccgtaatcaa ggccgaacgc gttgccgagg cccaggtcat cttttccaaa atacccggat
     1261 aagtatatgt ggtcgttggg tcccagccgg taattcgcct ttgcgttgag gtcatagaaa
     1321 taaagccggc tgcggctggt gttcgtgtcc ttcgacagct tcaggaacag gtcggcgtaa
     1381 gtccttcggg cgctgacgat aaaggaaccg ccgccgttcg cgatcggacc ctccacgtta
     1441 agccgtgagg aaatgagccc cacaccaccg ctcacgacat aatcattgac attaccatca
     1501 ttcatccgca cgtccagcac cgaggcgagc cggccgccga attcagcggg gatcgcgcct
     1561 ttgtataacg tgacgtcctt aatggcatcg gagttgaagg tcgagaaaaa tcccagcaga
     1621 tgcgacgggt tgtaaacggt cgcctcatcc agcagaataa ggttttgatc cacgcttccg
     1681 ccgcgcacat aaaacccgct gctcccgtcg ccgcccgact ggacgcccgg caggagctga
     1741 atggtcttga gaatatcctt ttcgccaaag atgaccggga cgtccttgat ctcggtgatg
     1801 gaaagcttct ggacacccat cagcggcctg ctgacattat tggcgttgcg attcgccgtc
     1861 accacgacct cctgcaactg acgggcgcct gcggcgaggt tcacgttgat caccgtatcc
     1921 ctttgcagca aaagccggat cgtgtcgctg cgatagccgg taaaactaac caccatttgg
     1981 tatttccctg cggctgcgcg gatggaatag aacccatacg cgttactcag gacggcgtcc
     2041 cgcggatggt cagggaacaa tacgcttgct ccaatcagcg tctccccggt cgccaggtcg
     2101 cggacggtac cgctgacggt atgcggggcc tgggcaaaaa gaagcgatgg gatcgaataa
     2161 aaaaggacgg acaacaggaa ccggtacact tgctcagtac tgatcattcg catctgtttc
     2221 agggatagca ggatttgtta atagatgaga tcgcttatcc agggtaccag ttacgcagcc
     2281 ggacatcgat gtcggggtct ccggccttga caagcttgct aaatccttct gtgtcgctaa
     2341 agccgccctg cccgcggtaa tggtagggat agacgatgcg gggtttgaag tccagcacgg
     2401 cacccgccgc ttccttaata tccatggtgt agggcaggtt catacaaaca aaagcgacgt
     2461 caatcccggt aagggcgcgc atttcaggta tcccctgcgt gtcgcccgaa agatacagtt
     2521 tattcccgcg cgcgctaagc acgtagccgt ttccccttcc tttcggatgc cgggggtcgg
     2581 ttgggcttgc aggaaggttg tacatcggga tggcggcgac accgataccc aggatttcct
     2641 tattctcccc gttcccgagg atcacgatgc ccgtcttctc cgccgccggc atcttgtcgg
     2701 caacggcttg cggcaccacc agcaccgttc cttcccttcg gatcgcgcga agcgtggccg
     2761 gatcgaaatg atcgccatgg atatcggtca ccaggatgat atccggcgcc gccaggcctt
     2821 catatttggc tgccccgccg acaggatcag cgtagatcgt tttaccggcc agggtcagca
     2881 cgacgctggc atggacgatg ggctgaacga tcagcgcgcc gcccgacagg gcgatcgtgt
     2941 ccggcgaaat ccgctgtgcg cttgaagcaa tgccgcccag caataaaaga aaaccaacca
     3001 atcctgattt catagcaagt agtttgatcg ggcaaaggta ccaaaaccgc gcgaggtcat
     3061 caataattga gggtgacgcc ggcgccgaaa cccatgtcgc tgtcataatg ggtcgacagg
     3121 ccgagccatt tggtgaggac gtaacgaaat cccgccat
//
LOCUS       DATGKE010000023         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_201548, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000023 DATGKE010000000
VERSION     DATGKE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201548"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VKU83_00380"
     CDS             <1..317
                     /locus_tag="VKU83_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HLI92026.1"
                     /translation="TWPEAVAATFPMGSMTGAPKNEVIRLIGLYEGQKRGLFSGAVGY
                     VTPGGDFDFNVVIRSLLYNQGSRYLSYQVGSGITAYSDPAAEYDECLLKAAGIRKALG
                     ME"
     gene            complement(377..1477)
                     /locus_tag="VKU83_00385"
     CDS             complement(377..1477)
                     /locus_tag="VKU83_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLI92027.1"
                     /translation="MCNKRLLLLGLTLVVLSGWVYPSNDPGPAAGHNFPHSSADTERL
                     AGLTAPGALAKANPQLTFSSELFLATEIGGTNGARLNPKALNFIEDYVKENWDELQLV
                     RTTGGPYFHLIENILAQYDLPKELKYLAVIESNLKPSARSRVGAVGPWQLMPQTARDL
                     GLKVNRNVDERRNYVKSTRAAALYLKDLYRQLGDWLLVIAAYNTGTANVFHAIKKSGS
                     RNFWDLQYYLPLESRIHVKKFIGTQYVFEGQGSVTTLTRDEAMDQLSGSAMYVFHRQL
                     SPAEVNGARTVTVSGKYTADVIARYVMMDSVEFNRYNPDFDKVMASSNNSYDLKLPAD
                     KMDLFEANKYQILTASMQQLNANNDLAQVAVK"
     gene            complement(1606..>2586)
                     /locus_tag="VKU83_00390"
     CDS             complement(1606..>2586)
                     /locus_tag="VKU83_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLI92028.1"
                     /translation="GSAVAVQAGLAMASLGSDTGGSVRQPADFCGILGLKPTYGRVSR
                     YGLIAYASSFDQIGIFGLTVPDVARVLEVISGPDDYDSTVARSAAQPCDDDLLMGKID
                     RNKKYRIAYLKEAISHPGLDPEIRRSILERLEQWRREGHTVTAVDFELFDYIVPAYYI
                     LTTAEASSNLARFDGVRYGYRTPAKGLDLTELYKRTRSEGFGREVKRRILLGTFVLSA
                     GYYDAYFTKAQQVRRRVLDCTNLIFKEFDILVLPTAPSTAFEQGEKIKDPIAMYLADI
                     YTVYANLTGIPGISLPLFTHTNGMPFGIQAMASRFKELSLLQFSHQMMQH"
BASE COUNT          578 a          708 c          716 g          584 t
ORIGIN      
        1 tgacctggcc tgaggccgtc gccgcgacat tccccatggg gtccatgacc ggggcgccga
       61 aaaacgaggt gatccggttg atcggcctgt acgaaggcca gaagcgcggc cttttttccg
      121 gcgcggtggg atacgtaacg ccgggcggcg acttcgattt caatgtcgtg atccgaagtt
      181 tgctctataa ccagggcagc cggtatcttt cctaccaggt tggctcgggc atcactgcct
      241 acagcgaccc ggcggctgaa tacgatgagt gtctgctgaa ggcggccggc atccgtaagg
      301 cgctggggat ggaataaaaa tacagaagcc ggcggatttc tccgaccggc ctcgtatcgt
      361 acataaacga gttgatctat ttgaccgcaa cctgggcgag gtcattattg gcgttgagct
      421 gctgcatcga agccgtgagg atctggtatt tattggcttc gaagaggtcc atcttgtctg
      481 ccggcaactt caggtcgtag ctgttattgc tgctggccat aactttgtca aagtccgggt
      541 tatagcgatt gaattcgacg gagtccatca tgacataccg ggcgatcacg tccgcggtgt
      601 acttgccgga aacggtcacg gtccttgctc cgttcacttc agccggactc aactggcggt
      661 ggaacacgta catggccgat cccgacaact gatccatcgc ctcgtcacgg gttagcgtcg
      721 taacgctgcc ctggccctca aatacatact gcgttccaat gaatttttta acatggatcc
      781 gcgattccag cggcaaataa tattggaggt cccagaaatt ccggcttccg ctctttttga
      841 ttgcatgaaa gacattcgcc gtgccggtgt tgtaggcggc gatcaccagc agccagtctc
      901 ccaactgccg gtagaggtcc ttcaggtaca gcgctgccgc ccgggtgctc ttgacgtagt
      961 tccgccgttc atcgacattt ctgttgacct tcaaacccag gtcgcgggcc gtttgcggca
     1021 tcagttgcca gggacccacg gcgcctaccc ttgaccttgc ggaaggcttc agattggact
     1081 cgatgacggc caggtatttc agttcctttg gcaggtcata ttgggccagg atattttcga
     1141 tcagatgaaa ataagggccg cccgtggtgc gtaccagctg cagctcatcc cagttttctt
     1201 ttacgtaatc ttcaatgaaa ttcagcgctt taggatttaa ccgggcgcca ttggtcccgc
     1261 cgatctcggt cgccaggaaa agctctgagg aaaacgtgag ctgcggattg gcctttgcga
     1321 gtgcgccggg ggctgtcaag cccgccagtc gctccgtgtc cgcggaggaa tgaggaaaat
     1381 tatgccctgc cgcagggccg ggatcgttcg aagggtacac ccatccggag agaacaacga
     1441 gggtgaggcc caacaataaa agtctcttgt tgcacataat gtccaacgtt ttaagatggt
     1501 aaacagccca tatcggcgct tacaatcttt cataggttcg gacgaacgaa gggagatttc
     1561 gcttcacatc tctcagccac catagagtag gattttcgta ggactttaat gttgcatcat
     1621 ttgatgcgag aactgaagca aagataactc tttaaatcgg cttgccatag cctgaatacc
     1681 aaaaggcatg ccatttgtgt gggtaaataa ggggagggag atcccgggaa tgccggtcag
     1741 gttcgcgtaa acagtatata tatcagcaag atacatagca ataggatctt taatcttttc
     1801 gccctgctcg aaggcggtag aaggcgcggt aggcaggacc aggatatcaa actctttgaa
     1861 aatcagattg gtgcaatcga gcacccgtct tctgacctgc tgggcctttg taaaataggc
     1921 gtcgtagtat ccggcactta acacgaaggt tcccaataag atacgccgct ttacctcccg
     1981 gccgaacccc tcggaccggg tccgcttgta aagttcggtc aggtccagtc ctttcgccgg
     2041 ggtgcgataa ccataccgga cgccatcgaa ccgggcaagg ttcgacgagg cctcggcggt
     2101 cgtcagaatg taataagcgg ggacgatgta gtcgaacaat tcaaagtcaa cggcggtcac
     2161 cgtatgccct tcccggcgcc attgctccag ccgttccagg atcgaccggc ggatctcggg
     2221 atccagtccc ggatggctta tcgcttcctt gagataggca atccggtact ttttattgcg
     2281 gtcaatcttt cccatcagca ggtcatcgtc gcaaggttgg gcagccgagc gggctaccgt
     2341 gctgtcataa tcatccggtc ccgagatcac ctcaaggacg cgcgccacgt caggaacggt
     2401 caggccgaaa attccgatct ggtcaaaaga cgaggcatag gcaatcaaac catagcggga
     2461 aacccgccca tacgtcggct tcagccccag gatgccgcaa aaatccgccg gctgacgcac
     2521 cgaccccccg gtatcgcttc ccaggctggc catggcaagg cctgcctgga cggccaccgc
     2581 ggaacc
//
LOCUS       DATGKE010000024         5719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1136589, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000024 DATGKE010000000
VERSION     DATGKE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136589"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /gene="manA"
                     /locus_tag="VKU83_00395"
     CDS             <1..955
                     /gene="manA"
                     /locus_tag="VKU83_00395"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HLI92029.1"
                     /translation="LDVKDMLSIQVHPSKAAAELEFAAENERGLDITSALRNYKDDNH
                     KPELMSALSEFWLLHGFKPEEELIRLLADTPELNFLMPGFATGGYQQLYKSVMEMPQA
                     QVNEILQPLLDRILPMYRGGELVKTQEHFWAARAALTYDGAGRIDRGIFSIYLFNLLN
                     LHPGEAIFQDAGLPHAYLEGQNVEIMANSDNVLRGGLTPKHVDVAELLKHVRFEATTP
                     RIIVGESDAGHIKVYSTPAPDFELSRISLMAGEAVGVKAHSVEIFFVMEGLVGVIEEG
                     GQPFSRRRGDSFVAFHGARFQLKAQDKDAVLYRAAVPAGKA"
     gene            complement(989..1462)
                     /gene="rnhA"
                     /locus_tag="VKU83_00400"
     CDS             complement(989..1462)
                     /gene="rnhA"
                     /locus_tag="VKU83_00400"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HLI92030.1"
                     /translation="MSNELTMYTDGASRGNPGPGGYGTILFWGGHKKELSAGYRRTTN
                     NRMELMAVIAGLKALKKDNQRITIYSDSQYVVKAVEHGWLKNWVATNFKGGKKNKDLW
                     LEFYELARKNSIRFVWVRGHADNPHNNRCDELATAAADGTGLLVDEEFEKESPGA"
     gene            complement(1577..3214)
                     /locus_tag="VKU83_00405"
     CDS             complement(1577..3214)
                     /locus_tag="VKU83_00405"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HLI92031.1"
                     /translation="MNTDTLDFNKNEDALRKPVSNWRGRLEKIMLGGGKAAIERQREK
                     NKLTARERIQYLIDEGSHFIEIGAFAGYGMYEEQGGCPAGGTVAGVGYISGRQCVILA
                     NDQTVKAGAWFPITGKKNLRLQEIAMENHLPVIYLVDSAGVFLPMQDEIFPDKEHFGR
                     IFRNNARMSAMGITQIAVVTGPCVAGGAYLPIMSDETIMVEGNGSIFLAGPYLVKAAV
                     GEQVDAETLGGAVTHTEISGIADYKFATEKECLDQVKKIIGKLGHPPAAPFDRGTPRA
                     PAHPVEELYGLFPSDATRPYDMLEIIRRIVDDSAFVQFKEEYGKTILCGYARIDGWAV
                     GIVANQRKMVKTRKGELQMGGVIYNDSADKAARFIMNCNQKKIPLVFLQDVTGFMVGS
                     RSEHAGIIKDGAKMVNAVANSVVPKITIILGNSYGAGNYAMCGKAYDPRFIYAWPTAR
                     IAVMGGEQAAKTLLQIQVSAMKSKGKQIDPVEEKRLLDEIKGRYDEQTSPYYAAARLW
                     VDAIIDPADTRRVIAEGLNAAAHNPTVPDFSTGVIQV"
     gene            3299..4453
                     /locus_tag="VKU83_00410"
     CDS             3299..4453
                     /locus_tag="VKU83_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HLI92032.1"
                     /translation="MSRRRKITWIVLIVLAVLIRLVSMFPGAVEQYYSRGFYPVLARL
                     QRWLFGWVPFSVGDVLYFAAAVLLLIRLARLIQLAVRRKLAKGWVWPVTRRGAFVLLC
                     LYVVFNLAWGLNYNRLGIADQLGLDVRPYSTADLDTLAEVMLSELTRLDTPARLRRAD
                     LGHMSQLRMGAGRAYGALWQNEGRAADFFFLDSPGLPFPRLSVKPSLFEYPGLYIGFA
                     GYYNPFTGEAQVNTLDPLFSQPYTICHEMGHQLGYAKENEANFIGFLAARSSQDPAFG
                     YSAYMDMYLYALRELYVRDSVLAKTFKGRLPPEVRADFVRQQRFNLKYENPFEPVIWA
                     IYGRYLRANRQPHGMVTYSEVTAWVIAYAKKYGWSSLKAWRAAARYPPGG"
     gene            complement(4463..4993)
                     /gene="dcd"
                     /locus_tag="VKU83_00415"
     CDS             complement(4463..4993)
                     /gene="dcd"
                     /locus_tag="VKU83_00415"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLI92033.1"
                     /translation="MSDTRILEEIKKGTIKIEPYDRGCLGSNSYDVHLGKWLATYREH
                     ILDAKKHNEIEYFEIPDEGFVLYPHIFYLGVTDEYTETHEHVPFLEGKSSTGRLGIDI
                     HATAGKGDVGFCGNWTLEISVKQPVKVYKGMPVGQLIYFPVDGEIEVKYNQKRNAKYH
                     GQSDRPVESMMWKNIF"
     gene            5044..5709
                     /locus_tag="VKU83_00420"
     CDS             5044..5709
                     /locus_tag="VKU83_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLI92034.1"
                     /translation="MPLFYQHNINRGTKLGIWRIEEPESFFLAKVPLKRDVTHPFKRR
                     QHLAGRFLLSHLFPDFPVEEIRVADTRQPYLEGDSYHFSISHCGAFAAAIVSRNCRVG
                     VDIELVTPRISRVAEKFLNEGEMTFFNDDYAVFLEQWGLRGRVEQELLTLIWSAKEAL
                     FKWHGQGELDFKRHMQLTGNIRMEGDTIKLPFAFEKDEIILLDLDARIFDEQALTLAW
                     VVS"
BASE COUNT         1150 a         1467 c         1721 g         1381 t
ORIGIN      
        1 cctggatgtg aaagacatgc tatcgattca ggtgcatccg tcgaaggccg ctgccgaact
       61 ggaatttgcc gctgagaacg agcgggggct cgatatcacc tccgccctcc ggaactataa
      121 ggacgacaat cacaagccgg agctgatgtc ggcactgagt gaattctggc tattgcatgg
      181 gttcaaaccc gaggaagaac tgatccggtt gctggcggac acgccggaat tgaactttct
      241 gatgccggga ttcgccaccg gcggttatca gcaactatac aagtccgtga tggagatgcc
      301 ccaggcgcag gttaacgaga tcttgcaacc gctgctcgac cggatcctgc ccatgtatcg
      361 gggcggggag ttggtgaaga cccaggagca tttctgggca gcccgggcgg cattgactta
      421 tgacggcgcc ggacggattg acagaggtat attctcgatc tatttgttca acctgctgaa
      481 cctgcatccc ggcgaggcga tctttcagga tgccgggttg ccgcatgcgt acctggaagg
      541 acagaatgtg gagataatgg caaattcgga caatgtattg cgcgggggtc tgacgccgaa
      601 acatgtagat gtagcggagc tgttgaagca tgtgcgattc gaggcgacga ctccgcgtat
      661 catcgttggc gagtccgatg cgggccatat taaagtatat tcgacgccgg cgcccgactt
      721 cgagttgagc aggatcagcc tgatggcggg tgaggcagtg ggggtgaaag cgcattcggt
      781 cgaaattttc tttgtcatgg aggggctcgt gggggttatc gaagaaggcg gccagccgtt
      841 cagccgcagg cgcggggatt cgtttgtggc tttccatgga gcccgcttcc agttgaaggc
      901 gcaggacaag gatgccgtgc tgtacagggc agcagtaccg gcggggaaag cttagtcccg
      961 ggaaagggag gtagtatcca ttcagcggtc aggctccggg ggattccttt tcgaattctt
     1021 cgtcgaccag gaggccggtg ccgtcggcgg ctgccgtggc cagttcgtcg cagcggttgt
     1081 tatgcgggtt gtcggcatgg ccgcgaaccc agacgaaccg gatgctgttt ttcctggcga
     1141 gctcgtaaaa ttcaagccat agatccttat tcttttttcc acctttaaag ttagtcgcga
     1201 cccagttctt cagccatccg tgttccacgg ccttgaccac atattggctg tcggaataga
     1261 tagtgatgcg ctgattgtcc ttcttcaggg ctttgagacc cgcgatcacc gccattagtt
     1321 ccatgcggtt gtttgtggtc cggcggtagc cggcggacaa ttccttctta tggcctcccc
     1381 aaaacaggat ggtcccatag ccgccagggc cggggtttcc gcgggaagct ccatcggtgt
     1441 acatggtcag ttcgttggac atgcggcaaa gatagggttt gaagggaatg tcggcgggat
     1501 tggccgggcc ctgaggccgt gtcgcgcggc cggatgcgaa ggccgcgctc cgaggcggtg
     1561 tcgcgcggca ggatcctcag acctggatca cgcccgtgct gaaatcgggc acggtgggat
     1621 tgtgagcggc agcatttaga ccttcggcga taacgcgccg cgtatcggcc gggtcaatga
     1681 tggcatcaac ccagagccgt gcggcggcat agtaggggga tgtctgttca tcgtagcgac
     1741 ccttgatctc gtccagcagc cgtttctctt caacggggtc gatttgtttt ccttttgatt
     1801 tcattgcgct gacctggatc tgcagcaggg tctttgccgc ttgctcgccg cccatcacgg
     1861 caatgcgggc tgtcggccag gcatagatga aacgcgggtc ataggctttg ccacacatgg
     1921 cataattgcc ggcgccatag gaatttccga ggatgatggt gatcttcggg acgaccgagt
     1981 tggcgaccgc gttgaccatt ttcgcgccat ccttgatgat gcctgcgtgc tcgctgcgcg
     2041 agcctaccat gaaaccggtg acatcctgaa ggaagacgag cgggatcttt ttttggttgc
     2101 aattcatgat gaagcgggcg gccttatcag cgctgtcgtt atagatcacg ccgcccattt
     2161 gcaattctcc ttttcgggtt ttcaccattt ttcgctggtt tgcgacgatg ccgaccgccc
     2221 agccgtcgat acgggcgtac ccgcatagaa tggttttgcc atactcctcc ttgaattgca
     2281 cgaacgccga gtcgtcaacg atccggcgga tgatttccag catatcgtac gggcgggtgg
     2341 cgtcggaagg gaaaaggcca taaagctctt cgacaggatg cgcgggagcc cttggcgtgc
     2401 cgcggtcgaa aggggcggcg ggagggtggc cgagcttgcc gatgatcttt ttgacttggt
     2461 cgaggcactc tttttcggta gcaaatttgt aatccgctat tccggaaatc tccgtgtgcg
     2521 ttacggcgcc gccgagcgtc tcggcgtcga cctgttcgcc aactgcggct ttgacgaggt
     2581 aggggccggc aaggaagatc gatccgttgc cttcgaccat gatcgtctcg tcgctcatga
     2641 tcggcaggta ggcgccgccg gccacgcagg gtccggtgac caccgcgatc tgcgtgatgc
     2701 ccatcgcgct catgcgcgca ttattgcgga agatgcggcc aaagtgttcc ttgtccggga
     2761 agatctcatc ctgcatgggg agaaaaacac ccgcgctgtc gacaaggtat atgacgggaa
     2821 ggtgattctc catcgcaatc tcctgaagcc tcaggttctt tttgccggtg atggggaacc
     2881 aggcgccggc tttgacggtc tggtcattgg cgaggatgac gcactgccgg ccgctgatat
     2941 agccaacgcc ggccacggta ccgccggcag ggcagccgcc ttgttcctca tacatgccat
     3001 agcccgcaaa agcgccgatc tcaatgaaat gggagccctc gtcgataagg tattgaatgc
     3061 gttcgcgagc cgtaagtttg ttcttttccc gctggcgttc gatggccgct tttccgccgc
     3121 ccagcatgat cttctccaac cggccgcgcc agttgcttac gggtttgcgc aaagcgtcct
     3181 cgttcttatt aaaatccagg gtatccgtgt tcattcagac aaatgttgaa tggcctcgcg
     3241 gccggtacgg gtgcaattta acatggatgc cgtaatattt tggagtattt ttaaggccat
     3301 gtcccgaagg cggaaaatca cctggatagt tctgatcgta cttgcggtgc tgatcaggct
     3361 tgtgtccatg tttcccggtg ctgtcgaaca atattattcg cggggatttt atccggtgct
     3421 ggcaaggttg cagcgctggt tattcggctg ggtccccttc agcgtcgggg atgtcctgta
     3481 ttttgcggca gcggtcctgc tgttgatcag gttggcgagg ctgatccagc ttgcggtaag
     3541 gcggaagctg gcaaaaggct gggtgtggcc ggtgacaaga aggggcgctt tcgtgttgtt
     3601 gtgcttatac gttgtcttca atctcgcctg ggggctgaac tacaaccggt taggcatagc
     3661 cgatcaattg ggactggacg tcaggccgta ttccacagcg gatctcgata ccctcgcgga
     3721 agtaatgctc tcggaattga cccggctcga cacccccgcg cggctccgcc gggcggacct
     3781 gggacacatg agtcaattga ggatgggagc ggggagagct tatggcgctt tatggcagaa
     3841 tgaagggagg gccgcagatt tttttttcct ggatagccca ggccttccct ttccgcggct
     3901 ctcggtgaag ccgtcgttgt tcgaatatcc ggggctttat atcggtttcg ccggctacta
     3961 taatccattc acgggcgagg cgcaggtgaa cacattagac ccactgttca gccagcctta
     4021 tacaatctgc cacgagatgg ggcatcaact cggatatgcg aaggaaaacg aggctaattt
     4081 tataggattc ctggctgcca ggagctcgca ggatcccgct tttggctact ccgcatatat
     4141 ggatatgtat ttatacgccc tgcgtgaatt gtatgtgagg gattcggtgc tcgcgaaaac
     4201 cttcaagggg cgcctcccgc cggaggtgcg ggcggatttt gttcggcagc agcggttcaa
     4261 cctgaagtat gagaacccct tcgagccggt gatctgggcg atatacggcc ggtatttgcg
     4321 ggccaaccgc cagcctcatg ggatggtaac ctattcagag gttaccgcct gggtgatcgc
     4381 ctacgcgaag aaatacggct ggagctcgct gaaggcctgg cgtgcggcgg cccgctatcc
     4441 tccaggcggg taaagcaaag gatcagaaga tattcttcca catcatgctt tcgaccggcc
     4501 ggtcggactg gccgtggtac ttagcgtttc gcttctgatt gtatttaacc tcgatctcgc
     4561 cgtctacagg gaagtatatg agctggccca caggcattcc cttatatact tttacgggtt
     4621 gtttcaccga gatctcgagc gtccaattgc cgcaaaagcc cacgtcgccc ttgcccgctg
     4681 tggcgtggat atcgatgcca agccgtccgg tggacgactt tccttcgagg aatggcacat
     4741 gctcatgcgt ctccgtgtac tcgtcggtga cgccaagata aaaaatatgc gggtacagca
     4801 cgaatccctc atcaggaatt tcgaaatatt cgatctcgtt atgtttcttt gcatcgagga
     4861 tgtgttcacg gtacgttgca agccatttgc cgagatggac atcataagaa ttgctaccca
     4921 ggcagcccct gtcatagggc tctattttta tcgtcccttt ttttatctct tccaggatgc
     4981 gagtatcgga caagatcata tgaaattggt taagtttgtg aatatgctat tataattcaa
     5041 gaaatgccgc tgttttatca acataatatc aaccgtggga caaaattagg tatttggcgt
     5101 atcgaagagc cggaatcctt ttttttggcg aaagtgccgc tgaagcggga cgtgacgcac
     5161 ccgttcaaaa gacgacaaca tttggcgggc cggttcctct tgtcccatct tttccctgat
     5221 tttccggtgg aagagatccg ggttgcagat acgcggcagc catacctgga aggggactcc
     5281 tatcattttt ccatttcgca ttgcggagct tttgccgccg ccatagtgag ccgcaattgc
     5341 agggtgggcg tggatatcga actggtaacc ccgcgcatca gccgcgtcgc ggagaaattc
     5401 ctgaacgagg gcgagatgac attttttaat gacgactatg ctgtttttct cgaacaatgg
     5461 ggattgcggg gccgggtgga gcaagagctc ctgaccttga tatggagcgc caaagaggcg
     5521 cttttcaaat ggcatgggca aggggagctg gattttaaac ggcatatgca actgaccggg
     5581 aacatccgga tggaggggga tacgatcaag ctaccgtttg cgttcgaaaa ggatgagatc
     5641 attttactcg acctcgatgc gaggatcttc gacgagcagg ccctgaccct cgcctgggtc
     5701 gttagttagg ttgcggcca
//
LOCUS       DATGKE010000025         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1547158, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000025 DATGKE010000000
VERSION     DATGKE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547158"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VKU83_00425"
     CDS             complement(<1..492)
                     /locus_tag="VKU83_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLI92035.1"
                     /translation="MRYGYWMPVFGGWLRNVDDENMEASWDYVKRLAIRSEEIGFDLS
                     LIAELNLNDIKGIEAPSLDAWSTAAALAAVTQRQELMVAVRPTFHNPALLAKQAANID
                     HISRGRLSLNVVSSWWRDEATKYGVGFEQHDDRYARTAEWLRVLNGVWNENGFSFEGK
                     YYKV"
     gene            complement(506..1498)
                     /locus_tag="VKU83_00430"
     CDS             complement(506..1498)
                     /locus_tag="VKU83_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92036.1"
                     /translation="MSPFAIYHEHPDWFKPLFEELDRRGIPFIRLNPAAHQFAIEGAA
                     PAYSLFFNRMSPSAYLREGIQGIFYTLNYLKYLEYHRIPVVNGYQCFTYETSKALQLL
                     LMQSLGIKYPRAHVVNHPSKLIEAAAGLRFPVVVKANIGGSGAGITKYHTPEDLRQAV
                     SAGQMNFGVDHTALVQEFIPARGGYITRVETLGGKYLYGIRVYLTGDTFNLCPADICQ
                     TSSGVELVRNACAIEAPKNGLKVEGYTPPAPVIKAIETIVQHAGIDVGGVEYIIDDRD
                     GELLYYDVNALSNFVADAVNVIGFNPHQRLVDYLERRAGGPVSTTAGEAFFSHA"
     gene            1655..4648
                     /locus_tag="VKU83_00435"
     CDS             1655..4648
                     /locus_tag="VKU83_00435"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HLI92037.1"
                     /translation="MQKITAVPLFLFLYLAAAAQQAPYSACPGCWNPDSLGNHRVVLR
                     YSDTGRGVAHARIAWRRRDMRPEDKRIIVQDSATGRRIRNVSIRSVTRESGDILFEPV
                     SGAGIYYVYYLPYRNEGRSNYPRGVYWRPDTTADEAWQGRGGDWPVNTKVTGFQAIDS
                     FNSFYPMEVIATAAETEALRAKYRNDPFLLFPEDRGHPIRMRRDLPFRWVRAGPGAAF
                     EARMGLGEYYAYQIGLYALQPLTDVRVEFRPLVNARGETIPVSAMMCINTNGVSYDGH
                     PFVMNVDVPAGQIQPLWCVVRIPPKTPGVYTGTVAVLANGRRQQIRLRFEVGQRLVPD
                     GGVDHPAEMTRLQWLNSTLAQENTVIAPYTPLQIAGDTLIRLLGRDIRLNAQGLPGQI
                     RTFFTPEMTELTDHPTDLLTEGIHFHFVRRSDGKDMPLRPGQLRFTRRSAGTVSWTGE
                     SASDSLRLVVDGSLEFDGFLSYTVKVIALTDLDLKDIAMHIPFEPAASAYMMGLGRKG
                     GYRPDSMYWWKWDVAHKNQDGAWIGTVNAGLQYSLRDEHYVRPLNTNFYLQKPLIAPT
                     SWANGDRGGIDIGIKGRSMLVNNYSGARQMRRGDTLFYNFTLLVTPFHPLNTDFQWAT
                     RFYHHYNDVDSIKATGATIINIHQGTPINPWINYPFIEWRRMKEYIDSAHHLGMKVKI
                     YNTIRELSDHAYEVFALRSLGHEVYSPGPGGGFSWLQEHVGEDYIPGWFEPAIKDAAI
                     INSGMSRWHNYYVEGMNWLVKNVGIDGIYLDDVAFDRVTMKRIKRVLTADGHPGIIDL
                     HSANQYNPRDGYNNSANLYMEHFPYLNRLWFGEYFDYEHNPPDFFLTEVSGIPFGLMG
                     EMLEGGGNPWRGMIYGMTNRMPWTDNADPRPIWKLWDNFGMQGTRMLGYWSPNCPVRT
                     SDSAVLATVYEKKGAALVSLASWAAQDVTVALHIDWGKLGIDPAHAVIEAPPVKNFQP
                     ARTFRVGEAIPVEKGKGWLLVIRGS"
     gene            complement(4664..5713)
                     /locus_tag="VKU83_00440"
     CDS             complement(4664..5713)
                     /locus_tag="VKU83_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92038.1"
                     /translation="MDRPLKILHLEDLPSDAEMVDRVLRKAQLRFEKKVVIDKPDFLK
                     ALREFQPDIILSDHSLPAFNSLEALKITREEGNQSPFILVTATVSEEYAVNVIKEGAS
                     DYILKDRLERLPNAVQNALDRRRTEEELVRQRDRQQKLVMETSIQVQEREREEIGKEL
                     HDNINQILAAAKLYLEIAIKDDDDSRLEAMEKSQQNLTLAMEEIRKLSQSLVAPSVGG
                     LGLDQALRKLLENFPVGESLEVDLDTSGYTEDISNDDIKLTCYRIAQVQLNNIVKHAR
                     ASKASILLDRVADRLVMTIRDDGVGFNTSGKTAGIGLRNIRNRVGFYNGVVLIDSEPG
                     RGCTLTVSIPIGSRA"
     gene            complement(5719..6171)
                     /locus_tag="VKU83_00445"
     CDS             complement(5719..6171)
                     /locus_tag="VKU83_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92039.1"
                     /translation="MINQVEVLLVEDNIHDAEMTIRSLKKVNLANNLVHVKDGQEALD
                     FIFGEGKFAGRDLVSGPKVILLDIKMPKVDGIEVLRRLKSREVSRAIPVVIMTSSKEE
                     QDIISSYELGVNSYVVKPVDFEGFAKAVSQLGLYWLLTNQLPMPSTKK"
     gene            complement(6190..>6939)
                     /locus_tag="VKU83_00450"
     CDS             complement(6190..>6939)
                     /locus_tag="VKU83_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92040.1"
                     /translation="MAVQVADARHSLEEQVKARTAELEAANKELEAFSYSVSHDLRAP
                     LRAVSGYAMMLKEDYEHSFDAEAKRITGNIISNVKMMGRLIDDLIAFSRLGKREVTRR
                     TVDMKALTEACVTELLHGWPDGKFNIVVGDMPPCLGDEDLLKQVWINLIGNAIKYSSR
                     TGDPFIEIGHTGRNGSSTWFVRDNGAGFDMKYSDKLFKVFQRLHSQEEFEGTGIGLAL
                     VRRIIDKHRGRIWAESEPGKGAVFYFTLPEQ"
BASE COUNT         1405 a         1927 c         2039 g         1568 t
ORIGIN      
        1 gaccttgtaa tattttcctt caaagctgaa gccgttttcg ttccatacgc cgttgaggac
       61 cctgagccat tccgcggtgc gtgcgtaccg gtcgtcgtgc tgctcgaatc cgacgccata
      121 ttttgtcgct tcgtcgcgcc accaggaaga aacgacgttc agggacagtc ttcctctgct
      181 gatatggtcg atattggcgg cctgcttggc cagcagcgcc ggattatgga aggtcggacg
      241 gacggcgacc atcagttcct gccgctgtgt caccgcggcc aaggcggcag ccgtcgacca
      301 ggcgtcgagg gaaggggctt cgatcccctt tatgtcgttg agattgagct cggcgataag
      361 actgaggtca aagccgatct cttcgctgcg gatggctagc cgtttgacgt agtcccagct
      421 tgcctccata ttctcgtcat cgacgttccg aagccagcca ccgaagacgg gcatccagta
      481 tccatatctc atacggttca tttaatcagg catgtgaaaa aaaggcttcg ccggccgtcg
      541 tcgaaaccgg tccgccggcg cgtcgttcga ggtagtcgac aaggcgttgg tgtgggttga
      601 acccgatgac gttcaccgcg tccgctacaa agttcgacaa ggcgttgaca tcatagtaga
      661 gcaattctcc gtcgcgatcg tcgataatat actcgacacc gccgacatcg atgccggcat
      721 gctgcacgat cgtctcgatg gctttgatga ccggcgcggg tggcgtatag ccttcgacct
      781 tcaggccgtt cttcggcgcc tcgatcgcac aagcgttacg gacaagctca acaccggaac
      841 tcgtctggca gatatcggcc gggcaaaggt tgaaggtgtc gccggtcaga tagacacgga
      901 tgccatagag gtatttaccg ccgagcgttt cgaccctcgt aatatagccg ccgcgggcgg
      961 gaatgaattc ctgcacgagc gccgtgtggt cgacgccaaa attcatttgg cctgcggaaa
     1021 cggcctgtcg caggtcttcg ggcgtatgat acttggtgat acccgcgccg ctgccgccga
     1081 tattcgcctt cacgacgacg gggaaccgca atcccgcggc ggcttcgatg agctttgacg
     1141 ggtggttgac aacatgtgcc ctcgggtatt ttatgcccag ggattgcatc agcagcagtt
     1201 gcagcgcctt cgaagtctcg tatgtgaagc actgatagcc gttcacgacc ggaatccggt
     1261 gatactccag gtattttaag taattcagcg tgtagaaaat cccctggatt ccttcccgca
     1321 ggtaggcgga ggggctcatg cggttaaaaa aaagcgaata cgcgggagct gctccttcga
     1381 tcgcaaattg gtgggcggcc ggattcagcc ggataaacgg aatgccgcgc ctgtccagct
     1441 cttcaaagag aggcttgaac cagtcggggt gttcgtgata aatggcgaaa ggtgacatga
     1501 ggcgaagttt tgcgtgactg tgtgcaccgg tacagtgcct gcaaatatac gtcaagccgg
     1561 gtgctccggc gcattttttg ccggctggcg cggtcactgc gcggaaactt agtgggtcgg
     1621 ggaaagaaat cgggatgttg ccttatcttc aggcatgcaa aagatcacgg cagttccttt
     1681 gtttttgttt ttgtatctgg cagcggcggc tcagcaagcg ccctattccg cgtgtcccgg
     1741 ttgctggaat cctgactcat tgggaaatca ccgggtagtt cttcggtatt cggataccgg
     1801 ccggggcgtc gcgcacgcgc ggatcgcatg gcgtcgccgt gacatgcggc cggaagacaa
     1861 gcgcatcatc gtgcaggatt cagcgacggg gcgccggatc aggaacgttt ccatccggtc
     1921 cgtcacccgg gagtcaggcg atatcctgtt cgaacccgta tccggagcag ggatttatta
     1981 cgtctactac ctgccctacc ggaatgaggg cagaagcaat taccccaggg gcgtctattg
     2041 gcggcccgat acgacggccg atgaagcgtg gcagggccgc ggcggggatt ggcccgtaaa
     2101 tacaaaggtc acaggcttcc aggccatcga ttccttcaat tcgttttacc cgatggaggt
     2161 gattgccacc gctgcggaaa cggaagcgct gcgggcgaag tatcggaacg atccgttcct
     2221 gctctttccg gaagaccgcg gccatcccat ccggatgcgg cgcgatctgc ccttcagatg
     2281 ggtacgggcc ggcccgggcg cggcattcga agcccggatg ggcctgggcg agtattacgc
     2341 ttatcagatc gggctgtatg ctttgcagcc gctgacggat gtccgggtag aattccggcc
     2401 gctggtgaat gccaggggcg aaacgatccc ggtaagcgcg atgatgtgca tcaacacgaa
     2461 cggcgtaagc tatgacggcc atccgtttgt catgaatgtc gatgttccgg cgggacaaat
     2521 acaaccgctc tggtgcgtcg tccgcattcc gccaaaaacg ccgggcgtct acacgggcac
     2581 ggtagccgtg ctggcgaatg gccggcggca gcagatcagg cttcgcttcg aagtcggcca
     2641 gcggctggtc cctgacggcg gggtagacca tccggcagag atgacgcgcc tgcagtggct
     2701 caactcgaca ctcgcacagg aaaatacggt tattgcgccg tataccccgc tgcagatcgc
     2761 gggcgacacg ctgatcaggc tgcttggccg cgatatccgg ctcaacgccc aggggcttcc
     2821 ggggcagatc aggaccttct tcactccgga aatgacggaa ctgaccgatc accctactga
     2881 cctgctgacg gaaggcatcc atttccattt tgtccgccgg tcggacggga aggacatgcc
     2941 cttgcgcccg gggcaattgc ggtttacccg gcgatcggcc ggcaccgtta gctggacggg
     3001 agaaagcgcg tccgattcct tgcggctggt ggtggacggc tcgctggagt tcgacgggtt
     3061 tctctcgtac accgtcaagg tgatcgcgct gactgacctg gatctgaagg atatcgccat
     3121 gcatatcccg tttgaacctg ccgcatcggc atacatgatg ggactgggcc gcaagggcgg
     3181 ctaccggccg gacagcatgt actggtggaa gtgggacgtt gcgcacaaaa accaggatgg
     3241 cgcgtggatc gggacggtca acgcgggcct gcaatattcg ctgcgcgacg agcactacgt
     3301 ccggccactg aacaccaatt tttatcttca aaagccgctg atcgcgccga catcgtgggc
     3361 caacggggac aggggaggga tcgacatcgg gatcaagggg cgctcgatgc tggtaaataa
     3421 ttatagcggc gcgcgacaga tgagaagagg ggatacactt ttttacaatt tcaccctgct
     3481 cgtgacgccc ttccatccgc tcaacacgga ttttcagtgg gcaacgcgat tctaccacca
     3541 ctataacgac gtcgattcca taaaggctac cggcgctacc atcatcaata tccaccaggg
     3601 aacgccgatc aatccctgga tcaactatcc ctttatagaa tggcggcgca tgaaggagta
     3661 tatcgacagc gcccatcatc tcggcatgaa ggtaaagatc tacaacacca tccgcgagtt
     3721 gtcggatcat gcttatgagg tcttcgcgct ccggagcctg ggtcatgagg tttactcgcc
     3781 gggcccgggc ggcggcttta gctggctcca ggagcatgtc ggcgaagatt atattcccgg
     3841 ctggttcgaa ccggcgatca aggatgccgc catcattaat agtggcatga gccgctggca
     3901 caattactac gtcgaaggaa tgaactggct cgtgaagaat gtgggcatcg atgggatcta
     3961 ccttgacgac gtcgcttttg accgggtcac gatgaagcgg atcaagcggg tgctcacggc
     4021 ggacggccat cccggcatca tcgacctgca ttccgcgaat cagtataacc cgcgggatgg
     4081 atataataat agcgccaatc tgtacatgga acattttcct tacctgaacc ggttgtggtt
     4141 cggagaatat ttcgactacg agcacaatcc gcccgatttc tttctcacgg aagtcagcgg
     4201 catccctttc ggcctgatgg gagaaatgct cgagggcggg ggcaatccct ggcggggcat
     4261 gatctatggg atgacgaacc gcatgccctg gacggataat gccgacccgc ggccaatctg
     4321 gaaattgtgg gacaattttg gtatgcaggg aacccggatg ctgggctatt ggagtccgaa
     4381 ctgcccggtg aggacgagcg attcggccgt gctggccacc gtttatgaaa agaagggcgc
     4441 tgccctcgtg tcgctggcaa gttgggccgc ccaggatgtc acggtcgcgt tgcacatcga
     4501 ctgggggaag ctcggtatcg atccggcgca tgcggtcatc gaggcgccgc ccgtaaagaa
     4561 ctttcaacct gcccgtacct tccgggtggg cgaggcgatc ccggtggaaa aagggaaggg
     4621 ctggcttctc gtcattcgcg ggagctgaag ggctggcttc tggtcatgcg cgcgaaccga
     4681 tggggatgct gacggttagg gtgcatcctc tgcccggctc ggagtcaatg aggaccactc
     4741 cgttataaaa accgacccgg ttcctgatat tccgcaggcc gatgccggcg gttttcccgc
     4801 tggtgttaaa acctacgccg tcatccctga ttgtcatgac cagtcggtcg gctacgcggt
     4861 cgagcaggat cgacgcctta cttgcgcggg catgtttgac gatgttattg agctgcacct
     4921 gggctatccg gtagcaggtg agtttgatat cgtcgttgct gatatcttcc gtatagccgc
     4981 tggtatcgag gtcgacttcc agcgattcgc caaccgggaa attctcaagg agcttgcgca
     5041 gtgcctggtc caaaccgagg ccgccaacgg agggagccac cagggattgc gagagtttcc
     5101 ggatctcctc catggccagc gtaaggttct gctgactttt ttccattgcc tcgaggcggc
     5161 tgtcgtcatc atccttgatc gcgatttcca ggtaaagctt tgctgccgca aggatctggt
     5221 tgatattgtc atggagttcc ttgccgatct cttcccgctc cctttcctgg acctggatgc
     5281 tggtttccat taccagcttt tgctgccggt cccgttgcct gaccagctcc tcttctgtcc
     5341 ggcgacggtc cagcgcgttc tgcacggcgt tgggaagccg ttcgagacgg tccttcagga
     5401 tatagtcgct tgcgccttcc ttgatgacgt tcacggcata ttcttccgag acggttgcag
     5461 tgaccagaat aaaaggcgat tgattgccct cttcccgcgt tatcttaagg gcctccaatg
     5521 aattgaaggc cggcagggaa tggtcggaaa ggatgatgtc cggctgaaat tcccgcaggg
     5581 ctttcaggaa atccggtttg tcaatgacca cttttttttc gaaccgaagt tgtgccttcc
     5641 gcagtaccct gtctaccatt tcggcatcgg aaggaagatc ttcgagatgt aaaatcttta
     5701 aaggccggtc catgcgaatt atttttttgt tgaaggcatc ggcagctgat tcgtcagcag
     5761 ccaatacagg cccaattgac tcacggcttt ggcaaatcct tcgaaatcga cgggtttgac
     5821 aacataactg ttgacgccga gctcatagct gctgataata tcctgttctt ccttcgagga
     5881 cgtcatgatc acgaccggga tggcccggct gacctcgcgc gatttcagcc ggcgcaggac
     5941 ttcgatccca tcgaccttgg gcattttgat gtccagcagg atcactttcg gcccgctgac
     6001 cagatcgcgg ccggcaaatt ttccctcccc aaaaataaag tccaacgctt cctggccatc
     6061 ctttacgtgt acgagattgt tggcgaggtt gacctttttc agggaacgga tggtcatttc
     6121 ggcatcgtga atattgtctt cgactaagag tacttccacc tgattgatca tagtactatc
     6181 tttttttggc tattgttccg gcaacgtgaa atagaatacg gcgcctttcc ccggctcgga
     6241 ctcagcccag atcctgcccc ggtgcttgtc gatgatccgg cggacaagcg caagcccaat
     6301 ccccgtgcct tcgaactcct cctggctgtg caggcgctgg aagactttaa acagcttatc
     6361 cgaatatttc atatcaaatc ctgcgccgtt atcgcggacg aaccaggtgc tggaaccgtt
     6421 cctgcccgtg tgccctatct cgatgaacgg gtccccggtc ctggaggagt atttgatcgc
     6481 gttgccgatc aggttgatcc atacctgctt gaggaggtct tcgtcgccaa ggcagggcgg
     6541 catgtcaccg acgacaatat tgaatttgcc gtccggccag ccgtgcagca gctcggtaac
     6601 gcaggcttcc gtgagggcct tcatgtcgac ggtgcgccgg gtcacttccc gcttgcccag
     6661 gcgggagaaa gcgatcaggt cgtcgatcag gcggcccatc attttgacgt tggagatgat
     6721 atttcccgtg atgcgtttgg cttccgcgtc gaaactgtgc tcataatctt ccttcagcat
     6781 catggcatag ccgctgacgg cgcgtaacgg cgcccgcagg tcatgcgata ccgaataaga
     6841 gaatgcctcc agttccttgt tggcggcttc gagttcagct gtccgggcct tcacctgttc
     6901 ttccagggaa tggcgggcat ccgccacctg caccgccat
//
LOCUS       DATGKE010000026         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_97392, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000026 DATGKE010000000
VERSION     DATGKE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97392"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VKU83_00455"
     CDS             <1..599
                     /locus_tag="VKU83_00455"
                     /inference="COORDINATES: protein motif:HMM:NF044297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92041.1"
                     /translation="FSVLLYENGEASTRIAGTDAGELGLQVKCSTRFPRDGDVRFEIE
                     PEKEASFEIRFRIPDWSAGFKAKIGANTYQGKEGSWLTIQRRWKPGDVVWISFDMPAE
                     VLPGGASYPNAVAIRRGPQVLAIDKGLNAGIDSLGAVNFVSAGPLTDAAEALPAGWDW
                     REAFWLEAKVGNVSRKLVLVPFAEAGQKADPVEVWIAR"
     gene            610..1608
                     /locus_tag="VKU83_00460"
     CDS             610..1608
                     /locus_tag="VKU83_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92042.1"
                     /translation="MFIINNYPAAVACCIVTMFCWGSWANTQKMAAATWRFELFYWDY
                     VWGILLFSLLLAFTLGSTGSGGRAFIPDLAQAGGRNILSAITGGIVFNAANILLGAAI
                     AIAGMAVAFPVGIGLALVIGVVVNYVANPVGRAGMLGEGVGLIVLAILLNAFAYGRMQ
                     SAGSRRSGNGLLLAVAAGVLMGFFYKYVAASMFSNFNVPEPGKLSPYTAVVVFSAGIF
                     LSNLIFNSFLMRRPFAGEPVTYAAYFKGGPRNHITGILGGSIWCLGMSLSIIASGQAG
                     PAISYGLGQGATIVAALWGIFIWKEFKGAPNGTQRVLNLMLLFYLIGLGLLVYAGA"
     gene            1617..2531
                     /gene="rbsK"
                     /locus_tag="VKU83_00465"
     CDS             1617..2531
                     /gene="rbsK"
                     /locus_tag="VKU83_00465"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLI92043.1"
                     /translation="MKSYIVVVGSSNTDMVVKAERLPGPGETVLGGEFLMAAGGKGAN
                     QAVAAARLGADVCLIAKVGQDIFGRQAIDLFSREGIETGSIIVDAGAPSGVALINVDA
                     RGENCIVVASGANARLMPADVAAAADKITNAALILMQLEIPLPTVEFVAELARRNNVP
                     VVLNPAPARELTDDLLAKISILVPNQKEAEMLSGVRIADKAAAGKAARALAAKGVAKV
                     IITMGAQGALLLESDIVHWVPSPAVTAVDTTAAGDVFCGALAVAIAEGKPLKEAVEAG
                     CAAAALSVTKMGALTSAPTRAELDRFYQ"
BASE COUNT          525 a          720 c          927 g          590 t
ORIGIN      
        1 ggttctccgt tttgttgtat gaaaacggcg aagcaagcac gcggatcgcg ggtacggatg
       61 cgggcgagct tggcttgcaa gtgaaatgca gtacccggtt tccccgggat ggcgacgtcc
      121 ggtttgagat cgaaccggaa aaggaggcca gtttcgagat caggttccgc attccggact
      181 ggtcggcagg attcaaggcg aaaatcggcg cgaacaccta tcagggaaag gagggcagct
      241 ggctcactat ccaacggcga tggaaacccg gagacgtagt gtggataagc ttcgacatgc
      301 cggcggaagt attgccggga ggcgcttcgt acccgaatgc ggttgcgatc cggcgcggcc
      361 cgcaggtgct ggcgatcgat aaggggctga atgccggcat cgattcgctc ggggcggtaa
      421 attttgtgtc cgcgggcccg ctaacggatg ccgccgaggc cttgcctgcc ggatgggatt
      481 ggcgggaggc gttctggctc gaggcgaaag tgggcaatgt ttcccgaaaa ctggtgctgg
      541 tgccttttgc cgaggccgga cagaaggcgg acccggtcga ggtttggata gcgcggtgaa
      601 aattggctta tgttcatcat caataattat cccgcagcgg tggcctgttg cattgtcacc
      661 atgttctgct ggggctcgtg ggcgaacacc caaaagatgg cggcggccac ctggcgtttc
      721 gagctctttt actgggatta tgtatggggg atactgctct tctccctgtt actggccttc
      781 acactcggaa gtaccggtag tggcgggcgg gcctttatcc cggaccttgc ccaggcgggc
      841 gggaggaaca tcctatcggc gatcacgggg gggatcgtct tcaatgccgc caacatcctg
      901 ttgggcgctg ccatcgctat cgccggaatg gccgtggctt tcccggtagg gattggcctg
      961 gcgctggtca tcggcgtggt ggtcaattat gttgccaacc ctgtcggcag ggcaggcatg
     1021 ctcggagaag gtgtcggcct gatcgtgctg gcgatcctgc tgaatgcttt tgcgtacggg
     1081 cgaatgcaat cggcgggcag ccggcgatcg gggaacggcc tgctgttggc cgtcgcggcg
     1141 ggcgtcctga tggggttttt ttacaaatac gttgcggcaa gcatgttcag caatttcaac
     1201 gtgcccgagc cggggaagtt gagcccatat accgcagtgg tggtgtttag cgccggcatt
     1261 ttcctgagca atctcatttt caattccttt ctgatgcgcc ggccatttgc gggcgagccg
     1321 gtgacgtatg ccgcctattt caaaggcggt cccaggaacc atatcaccgg catccttgga
     1381 ggatcgatct ggtgcctggg aatgtcgttg agcattatcg cctcgggtca ggcaggccct
     1441 gccatttcct atggactcgg ccagggcgcc accattgttg cggcactctg gggcatcttc
     1501 atctggaagg aatttaaagg agcgccaaac ggcacccagc gggtgctgaa cctaatgttg
     1561 ctgttttacc tgattggatt gggtttgctg gtttatgccg gggcatgact tgatttatga
     1621 aatcgtatat agtagttgtc ggcagttcca acacggatat ggtcgtcaag gccgagcggc
     1681 tgcccgggcc gggggaaacc gtgctgggcg gcgaatttct gatggctgcc ggcggcaagg
     1741 gagcaaacca ggcggttgct gcggcgaggc tgggggccga cgtgtgtttg atcgcaaagg
     1801 tcgggcagga tatcttcggg cggcaggcga tcgacctctt ctcgcgggaa ggcatcgaga
     1861 ccggctcgat aatagtggac gccggtgcgc cctcgggcgt ggcgctgatc aatgtcgacg
     1921 caagaggcga aaactgcatt gtggtagcct cgggcgcgaa tgccaggctt atgccggctg
     1981 acgtggccgc tgcagcggac aagatcacaa acgccgcgct gatcctgatg cagctcgaaa
     2041 tcccgctgcc aacggtggaa tttgttgcag aattggcccg caggaataat gtgccggtcg
     2101 tgctgaaccc cgcgcccgcc cgcgagctga cggacgacct gctggcgaaa atttcaatcc
     2161 tggttcccaa tcaaaaggaa gccgagatgc tgtcgggagt gaggatcgct gataaagccg
     2221 ccgccgggaa ggcggcgcgg gcgttggcgg cgaagggagt cgcgaaagtg atcattacga
     2281 tgggggcgca gggggcgctg ctgctggaaa gcgatatcgt ccattgggta ccctcgccgg
     2341 cggtaacggc agtcgatacg acggctgcgg gggatgtctt ctgcggggcg ctggcggtgg
     2401 caatagccga ggggaagccc cttaaggagg ctgtggaagc gggttgtgcg gccgcggcat
     2461 tgtcggtgac gaagatgggc gcgctgactt ccgcgccgac gcgtgccgag ctggatcgat
     2521 tttatcagtg aaagagcttt caaatcttta tatttggcta tgaacccgaa ttttccgggc
     2581 cgtcctaatc ggctgctacc ctttgtcgca ttgacactct ccctcagcct ctgcaaggca
     2641 caaaccggcg gcacaacggt taccgaatgg caatatggca agaatggcgc cctatccatc
     2701 acttacgacg acgcttcacg ccatcagttt acgcgcgccc tgccgctcat ggaacgactg
     2761 aa
//
LOCUS       DATGKE010000027         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1234192, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000027 DATGKE010000000
VERSION     DATGKE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234192"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /gene="lptB"
                     /locus_tag="VKU83_00470"
     CDS             <1..632
                     /gene="lptB"
                     /locus_tag="VKU83_00470"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HLI92044.1"
                     /translation="GDVFLDDTNITRLPMYKRAQMGIGYLPQEASVFRKLSVEDNIRA
                     VLEMTKLTRADQKAKLEALLDEFHLHHVRKNNGDSLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGVDPIAVEDIQSVVARLKFRNIGILITDHNVNETLSICDRAYLLIDGKIFKH
                     GSAEELAEDEQVRRLYLGRNFELKRKDYLHEEARKQEEKNALAQDNSKV"
     gene            712..>2039
                     /locus_tag="VKU83_00475"
     CDS             712..>2039
                     /locus_tag="VKU83_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLI92045.1"
                     /translation="MKLLSPAISRLARLRLGRIEHWMQNPVAVQREVLQHLVTSAQYT
                     EFGRKYNFSRIFSLREFKQAVPIHEYDDLKPYIQRIMNGEQNILWNTPISWFAKSSGT
                     TSDKSKFIPVSEESLQDGHYKGAKDVLTMYYDFNPDSDLLTGKGLVIGGSHTISQIKE
                     DVHYGDLSAVLLQNAPFWGNWIRTPELSIALLDEWEDKIERLAKNTINENVTSISGVP
                     TWTLVLIRRILEITGKHSLSEVWPSLELYIHGGVAFTPYQEQFRKLIGKDIYYLDMYN
                     ASEGFFAAQSNPYDSGMLLFLDHGIFMEFMPVEEYGKAQPETIGLADVELGHHYALVI
                     NTNGGLWRYLLGDTIQFTSRDPFRIRVSGRLKHFINAFGEEVIVDNSDKAIAIASEKT
                     GAVVNDYTAAPVYFGDQGNGAHEWLVEFEKKPSDLDAFVFELDKALQSIN"
BASE COUNT          514 a          552 c          533 g          440 t
ORIGIN      
        1 ccggggatgt gttcctggac gacaccaata tcacccgttt acccatgtac aaacgggccc
       61 agatgggtat cggctatcta cctcaggaag cttctgtttt ccggaagctc agcgtggagg
      121 acaatatccg ggccgtgctg gaaatgacga aactgacccg cgccgaccag aaagccaagc
      181 tggaggcatt gctcgacgaa ttccatctcc atcatgtccg caagaacaat ggcgactcgc
      241 tgagcggcgg tgagcggcgg cgtaccgaga tcgcccgcgc cctggcggtg gaccctaaat
      301 tcatcctgct tgacgagccg tttgccggcg tggaccccat agcggtggaa gatatccagt
      361 ccgttgtcgc ccggcttaag ttccgcaata tcggcatcct gatcaccgac cacaacgtga
      421 acgaaaccct ctcgatctgc gaccgggcct atctgctgat cgatggcaag atcttcaaac
      481 atggatcggc cgaagagctg gcggaggacg aacaggtaag acgactctat ctgggccgca
      541 acttcgagct gaagcggaag gattatctcc acgaggaagc ccgcaagcag gaggaaaaga
      601 atgccctggc gcaggataat tccaaggttt gacaaaccct gcttttcagt aagttatgta
      661 agaaattctc catgtcccgg cgattagtta ttttgctggt gaggaccatt tatgaagcta
      721 ttgagcccgg ccatatcaag actcgcgaga ttgcgcctcg ggcgcatcga acattggatg
      781 cagaaccctg tagccgtgca gcgtgaagta ttgcaacatc tcgtcacttc ggcacagtat
      841 accgaattcg gccgtaaata caacttctcc cgtatcttct cgcttcgcga attcaaacag
      901 gctgtcccca ttcacgaata tgacgatctg aaaccttaca tccagcggat catgaacggt
      961 gaacagaaca tcctctggaa tacgcccatc agctggttcg ctaaaagcag cggcacgacc
     1021 agcgacaaaa gcaagttcat ccccgtgagc gaggaaagcc tgcaggacgg tcactacaaa
     1081 ggtgcaaagg atgtgctcac catgtactat gatttcaacc cggattccga tctccttaca
     1141 ggcaagggcc tggtgatagg cggcagtcat accattagcc agatcaaaga agatgtgcat
     1201 tacggcgatc ttagcgccgt gctgttacag aatgcgccct tttggggaaa ctggatccgg
     1261 acgcccgagc tgagtatcgc actgctcgat gaatgggaag acaagatcga gcggctggca
     1321 aaaaatacca ttaacgagaa tgtcacttcc atcagcggcg ttcctacctg gacgctggta
     1381 ctgatccggc gcatcctgga gatcaccggt aagcacagcc tctcggaggt atggccgtcg
     1441 cttgagctgt atatccatgg gggggtcgcc tttactccct atcaggaaca attcagaaag
     1501 cttatcggaa aagatattta ctatctggat atgtacaatg ccagcgaagg gtttttcgcc
     1561 gctcaatcca atccatatga ttccggcatg ctgttgttcc tggaccatgg catctttatg
     1621 gaattcatgc ctgtcgaaga atacggcaag gcccaacccg aaaccattgg gcttgctgac
     1681 gtggagctgg gacatcacta tgcgcttgtc atcaacacca atggcgggct ctggcgctat
     1741 ttgctgggag acaccatcca gttcacctcc cgcgacccct tccgtatccg ggtgagcggc
     1801 cgtctaaaac atttcatcaa cgcctttggc gaagaggtca tcgtagataa ttcggacaaa
     1861 gccattgcca ttgccagcga aaagaccgga gccgtcgtca acgattatac cgccgcgccg
     1921 gtttattttg gcgaccaggg gaacggtgcg cacgagtggc tggtggaatt cgaaaaaaag
     1981 ccatccgatc tcgatgcctt cgtattcgag ctggataaag ctctccaaag cataaacag
//
LOCUS       DATGKE010000028         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_825450, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000028 DATGKE010000000
VERSION     DATGKE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825450"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VKU83_00480"
     CDS             complement(<1..870)
                     /locus_tag="VKU83_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92046.1"
                     /translation="MHLPVNSRIAQVYLLSKLKQTVIAILGVTFGISMYVFMNSFMRG
                     VNEAQNRLAFSTLAHVHIYNDRPADRSNLVSRAYPAGTIANIRNPKVIKYTEGIRNSQ
                     EIIRYLGEQPEVTAIAPEVNLNVFFKNVGNKVNGTLSGVNVAAEDRLFNLTSMMTEGS
                     WKELARRPDGIIVGAELAANLSLNTGDNLNILTSDGVTRTYKVIGKFRTNLASVDKTK
                     AYLSISATRQLLARNQDYVTDIQVNIKDFETARPFVRRVAPIIPYKVESWQMANAQLE
                     AGSNLRDAIALATS"
     gene            complement(870..1958)
                     /locus_tag="VKU83_00485"
     CDS             complement(870..1958)
                     /locus_tag="VKU83_00485"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLI92047.1"
                     /translation="MKRLPYIIVLLAAISCNPQEPVHPQYRNMVDAVFASGNIESKNQ
                     YHVTAYADGYLQQSFVAEDDSVQKGELLFRIAGEIQQTQVENAAANYRYALRNASDKS
                     LQVLQLESQIAQARKKLVTDSLNFLRFEHLLPTHAVAQVDYDNARLTYQASVSNLAVL
                     ENNLADLRTNLAQNAENTRAQFRIQQENDRYYQLTSDAPGRILNVYKRNGDLIRKGDI
                     VADLGTGQLLAKLEIAEEDIRRIQLGQPVLISLNTDKDHLYHATISKIYPSFDTGQQA
                     FIAEATFSEMPPSLRDGTQLQANIILNQKQHALVIPSSCLLPGDSVILAPSGDHRAVT
                     TGIHTLEWIEILGGIDSSTSLEPINSPK"
     gene            complement(1960..3273)
                     /locus_tag="VKU83_00490"
     CDS             complement(1960..3273)
                     /locus_tag="VKU83_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI92048.1"
                     /translation="MPRALFLAIFILWVSNAESQTVFHSLSEVWSAAIKNNPNQHIYR
                     LRTSQLTYEYRAAQSYLYPQISGAFSGTDNLKLNVTPVPGELIGQPGKTLYLTFGKHY
                     VYNGGVIATENLFDWQYVFHSNIARENITLNNLQQEAYQQSLKASAAQYYFSSLVAAA
                     SLRIADFDLRVADSILSAIGSRYAQGLVDIAAVHSAEINVNNVKQNIIQSSQLYTLSI
                     QNLKILTGLTARETLSLDERLDIDTLSADTSSALFPGPDKSLSVYAANVRLAELEAKS
                     EKAAAYPTLSISGFAGSQQFRDNFGLGFNNGDWNNYRYIQLNLGVPLFTGFYNRNKYR
                     SAETLARINAEQYRTAVEQGKINDSLLVTTYDNYRLLAAASARNLVLYRENVELSRQK
                     FAEGMISVDSFQETFEEYLRAENAHLNNLSSLFSAMSSIISRNSN"
BASE COUNT          707 a          819 c         1081 g          812 t
ORIGIN      
        1 ggaagttgcg agggcgatgg cgtcgcgaag gttcgaacct gcttccagct gggcattggc
       61 catctgccag gactccacct tgtaagggat gatgggcgca acgcgcctta cgaacggcct
      121 tgctgtttca aagtctttga tgttgacctg gatgtcggtg acgtaatcct ggttcctggc
      181 cagcagctgc ctggtagcgc tgatggaaag ataggctttt gtcttgtcga ccgaggccag
      241 gttggtgcgg aacttgccaa tcaccttata ggtgcgcgtc acgccgtcgg aagtgaggat
      301 gttcaggttg tcgcccgtat tcaggctgag gttggcggcg agctctgcgc ccacgatgat
      361 cccgtccggc cgccgtgcca gctctttcca gctgccctca gtcatcatgg aagtaaggtt
      421 gaaaagccgg tcttcggcgg cgacattcac gccggaaagg gtaccattga ccttgttgcc
      481 gacgttcttg aagaagacgt tgaggtttac ttccggggca atcgccgtga cctccggctg
      541 ctcgccgaga taccggatga tctcctggga attccggatg ccttccgtgt atttgatgac
      601 cttcggattg cggatattgg cgatcgtgcc ggcgggatag gccctgctca cgaggttgct
      661 gcgatcggcg ggcctgtcgt tatatatatg gacatgcgca agcgtgctga aggcaagccg
      721 gttctgggcc tcgttaaccc cgcgcatgaa actgttcatg aagacgtaca tcgagatgcc
      781 gaaggtcacc ccgaggatgg caatgaccgt ttgcttcagt tttgacagca gatagacttg
      841 cgcaatacgg gaattgaccg gcagatgcat tacttgggcg aatttatggg ttcgagggaa
      901 gtgctgctgt cgatgccgcc aaggatctcg atccattcca gggtatggat gcccgttgta
      961 acggcgcggt ggtcgccgga gggagccagg atgacagagt cgccgggaag aaggcaggaa
     1021 gacgggatca cgagggcatg ttgcttttgg ttcaggatga tattggcctg cagctgcgtg
     1081 ccgtcgcgaa gcgacggcgg catttcactg aatgtggctt ccgcgataaa tgcttgctga
     1141 ccggtgtcga aggaagggta gatcttgctg atcgtcgcat ggtagaggtg gtccttgtcg
     1201 gtatttagcg agattagaac gggctggcca agctggatgc gacggatatc ctcctcggca
     1261 atctctagtt tcgccagcag ctggcccgtg cccaggtcgg caacgatgtc tcccttccgg
     1321 atgaggtcgc cgttcctttt atagacgttc aggatccggc ccggggcgtc actggtcagc
     1381 tggtagtacc tgtcgttttc ctgctggatg cggaattggg cgcgggtatt ctcggcgttc
     1441 tgagcaaggt tggtgcgcag atccgcaaga ttgttttcca acaccgccag gttggagacg
     1501 gaggcctggt acgtcaggcg ggcattgtcg tagtcgacct gggcgaccgc gtgggttggc
     1561 agaaggtgtt caaagcgcag gaaattcagg gaatccgtca ccaacttctt ccgggcctgg
     1621 gcgatctggg actccagctg caggacctga agactcttgt ccgaggcgtt ccgcagggca
     1681 tagcgataat tggcagcagc attttcgacc tgggtttgct ggatctcacc ggcgatccgg
     1741 aaaagcagtt cgcccttctg cacgctgtcg tcctcggcga cgaaggattg ttgaagatag
     1801 ccatccgcat acgctgttac atgatactgg ttctttgatt cgatgttgcc gctggcaaaa
     1861 acggcgtcta ccatattgcg gtactgcgga tgaacgggtt cctgcggatt gcagctgatc
     1921 gctgcgagga ggacgataat ataaggaagg cgtttcatgt cagtttgaat ttctggaaat
     1981 gatggaagac atggcggaaa acaacgacga caagttattc aggtgcgcat tttcagcgcg
     2041 cagatactct tcaaaagttt cctggaaact gtcgacgctg atcatccctt ccgcgaattt
     2101 ttgccgggac agctccacgt tttcgcggta caggacgagg ttccgcgccg aggcggcagc
     2161 cagcaggcgg taattgtcat aggttgtgac gaggaggctg tcgtttattt taccctgttc
     2221 gacggcggtg cgatactgct cggcattgat gcgggcaagc gtctcggcgc tgcggtactt
     2281 gttgcggtta tagaaaccgg taaagagcgg aacgccgagg ttcagctgga tatagcggta
     2341 gttgttccag tcgccgttat tgaaacccag cccgaaatta tcccggaact gttgggaacc
     2401 ggcgaagccg cttatcgaga gggtgggata ggctgcggcc ttttccgatt ttgcctcgag
     2461 ttccgccagg cggacattgg cggcatagac actcaaactt ttatccgggc ccgggaaaag
     2521 ggcagagctg gtgtccgccg aaagcgtatc gatatccagt cgttcgtcga gggacagtgt
     2581 ctcccgggcc gtcaaaccgg taaggatctt caggttctga atggacaggg tatagagttg
     2641 actgctttga atgatatttt gtttgacgtt attgacattg atttccgcag agtgcacagc
     2701 agcgatgtcg acgagaccct gcgcataacg gcttccgatc gctgacagaa tgctgtcagc
     2761 gacccggagg tcaaagtctg caatacgaag ggatgcagcg gcgacgaggc tggaaaaata
     2821 atattgggcg gcggatgcct tcaggctttg ctggtaggcc tcctgctgca gattgttcag
     2881 cgtgatattc tcgcgggcga tgttggaatg aaagacgtat tgccagtcaa acaaattttc
     2941 ggtcgcgatg acgccgccat tataaacata gtgttttccg aaggtcaggt acagcgtctt
     3001 tcccggctgc ccgatgagtt cgcccggtac cggagtaacg ttcagcttga ggttatctgt
     3061 gccggagaat gcaccggaga tctggggata cagataggat tgggcggcgc ggtactcgta
     3121 ggtgagctgg ctggttctca ggcgatagat atgctggttg gggttatttt ttatggcggc
     3181 tgaccagacc tcggagagcg aatggaatac ggtttgcgat tccgcattgg atacccatag
     3241 aataaagatg gctagaaata aggcgcgtgg catgatacaa aggtcgccat ggccttcgtc
     3301 agccggaatg caaaccgggc gaactgtaca aaaagggcgg tggattgacg gctaggcttt
     3361 ttcgatccat tccaggaaag ggctcacttt aagccggctg acgagggggc ggtctgcgg
//
LOCUS       DATGKE010000029         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_306034, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000029 DATGKE010000000
VERSION     DATGKE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306034"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="VKU83_00495"
     CDS             complement(<1..1552)
                     /locus_tag="VKU83_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92049.1"
                     /translation="MSTLTDLLRPFSNLLAGRSVLSIGPGTESVRSFLEKAGAEVAGR
                     NMGQSDGNGTTAKFDFVVWVFSGGPRALAASCAEIAGFLKEEGSLLMATENPLGMSRA
                     LATAGRVRDNGDEPACGKHELVCLLTEAGLKNIDFYYPFPDMHEPAVLLDERAFETPA
                     LDLSNLIEPTARCGGKAASFTARRLMQLLIPNGLIMAHCNSFFLIASRKETRLLPREI
                     LGFCFNGSRMREYNKMNVFYLTGDHTIMVRRVPYEPDIRPKPGLPLVQELSEERYVPG
                     RLYTLVLEAILGRRGWSIADLRAWAQPYWALLLGYADEEGRVDGRYIDLAPFNLLLSN
                     GSLAAVDLEWKAAACLPVKYVFFRGLYHSFARVTAVRQPAAGTPTNIYRLILAVAGTL
                     FDDTGGLLDMFLQLEPFYFGAVFPRSDRFPPDRDLPVVPETAGHADSEARASEASTTD
                     ETATDATAADATPGDEPSGLCPLLACDLQVFVETADGAFSEEKSTSLRVALTPQRRIH
                     SIPLPWFTE"
     gene            complement(1554..1925)
                     /locus_tag="VKU83_00500"
     CDS             complement(1554..1925)
                     /locus_tag="VKU83_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92050.1"
                     /translation="MYAKINENGRQDWQSVDKRLGKIAEFIVKDLRLKQETIRLLMTQ
                     VQEMKAAMAAKDAHIRDLLEKLGKSSQDAEGLRQLMNKLLNDIENYQKDIDWYRRTYE
                     QRSLLGILKDKFFRKPDSSDQ"
     gene            complement(1942..>2217)
                     /locus_tag="VKU83_00505"
     CDS             complement(1942..>2217)
                     /locus_tag="VKU83_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI92051.1"
                     /translation="EGMPFAVLEALAMKKPVLLKDCVGNRDIVLKGINGDLFQSAGAA
                     IHKILRYYNNREMLHIMGEYSQRHCEREFDLTNMSAAYRDLYACRIR"
BASE COUNT          461 a          657 c          599 g          500 t
ORIGIN      
        1 tctccgtgaa ccagggcagc ggaatactat ggatgcgccg ctgcggcgtc aatgcgaccc
       61 gaagcgacgt cgacttctct tcgctgaatg cgccatcggc cgtttcgaca aacacctgca
      121 gatcgcaagc cagcaacggg cataatcctg agggctcatc gccgggggtc gcgtcggcgg
      181 cggtcgcgtc ggtggcggtc tcatcggtcg tggacgcctc gctggcgcgc gcctcgctgt
      241 ccgcgtggcc agccgtctct ggtaccaccg gcaggtcgcg gtcgggtgga aagcggtcgc
      301 tgcgtggaaa gacagcgccg aaatagaatg gctcaagctg aaggaacatg tccagcaggc
      361 cgcccgtgtc atcgaataac gttccggcca ccgcgaggat aagcctgtag atgttggttg
      421 gagtccctgc cgcgggctga cgaacggcgg tcacgcgggc aaaactgtga taaagcccac
      481 ggaaaaaaac atatttcacc ggtaaacacg ccgcggcttt ccattcgagg tccacggctg
      541 cgagtgaccc gttagataaa agcaaattaa acggcgccag atcgatgtat cgtccgtcca
      601 cccgtccctc ctcatcggca taacccagga gcagcgccca atatggctgg gcccaggccc
      661 gcaggtccgc gatggaccat ccccgtcggc caaggatggc ctccagcacc agggtataca
      721 gccgcccggg gacgtaccgc tcttcgctga gttcctggac gaggggaagc ccgggtttcg
      781 gccggatatc gggttcatac ggcacccttc tcaccatgat ggtatgatcg cccgtcaggt
      841 aaaaaacatt cattttattg tattccctca tccggctgcc attgaagcag aaaccgagga
      901 tctcccttgg cagcaagcgc gtttccttcc tcgatgcgat aaggaaaaat gaattacaat
      961 gcgccatgat caggccgttc gggataagca gttgcatcag cctccttgct gtgaagcttg
     1021 cagccttacc cccgcagcgg gccgtgggct cgataaggtt gctgagatcg agggcggggg
     1081 tttcgaaggc ccgctcgtca agcaataccg cgggttcatg catgtcgggg aaggggtagt
     1141 aaaagtcgat gttcttcaag ccggcctcag tcaacaggca aacgagctca tgcttcccgc
     1201 aagccggctc atcgccgttg tcccgaactc ggccggccgt cgccagcgcg cgagacatcc
     1261 ccaatggatt ttcggtcgcc atgaggagcg agccttcttc tttcaggaat cctgcaattt
     1321 cggcgcaact ggcggccagc gcacgcggcc cgccggaaaa tacccatacg acaaaatcga
     1381 atttcgcggt cgtcccgttc ccgtcggatt gccccatatt ccttcccgcc acctcggccc
     1441 ctgctttttc caggaagctg cggacggatt ccgtcccggg gccgatcgac agaaccgacc
     1501 tgcctgctaa cagattggaa aatggtcgca agaggtcggt tagcgtgctc atgttattgg
     1561 tccgaggaat ctggtttacg gaagaacttg tctttcagga tgcccaacag actacgctgt
     1621 tcataggtcc gccggtacca gtctatatcc ttctgataat tctcgatgtc attcaataac
     1681 ttgttcatca gttgacgaag gccttccgca tcctggctcg atttcccgag tttttccagc
     1741 agatcacgga tatgcgcgtc tttagctgcc atggcagctt tcatttcctg cacctgtgtc
     1801 atcaaaagcc ggatcgtctc ctgcttcagc ctcaggtctt tgacgatgaa ttctgcaatt
     1861 ttcccaagcc gcttgtcgac ggactgccaa tcctgccggc cattctcatt gatctttgca
     1921 tacatgtgat gtgctacatt tttatcttat ccggcaggca tataggtccc tataggctgc
     1981 ggacatattg gttaaatcga actcgcgttc acaatgccgc tgtgaatatt cgcccatgat
     2041 gtgcagcatc tcccggttat tatagtaccg gaggatctta tggatcgctg ctccggcgct
     2101 ttgaaacagg tcgccattta tccccttcag gacgatgtcc cggttgccga cgcaatcctt
     2161 gagcaatacc ggttttttca tcgctaatgc ttccaatacc gcaaagggca taccctc
//
LOCUS       DATGKE010000030         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_305993, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000030 DATGKE010000000
VERSION     DATGKE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305993"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VKU83_00510"
     CDS             complement(<1..261)
                     /locus_tag="VKU83_00510"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92052.1"
                     /translation="MERIIIDMDEVIADPMGEMIEWYRKEHRGDVDWEKMLVGSWVKG
                     FPDHHQPMVMDRLRAPGFFRHLPVMKDAVEVLREINDRYELFI"
     gene            complement(242..>2002)
                     /gene="uvrA"
                     /locus_tag="VKU83_00515"
     CDS             complement(242..>2002)
                     /gene="uvrA"
                     /locus_tag="VKU83_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLI92053.1"
                     /translation="FDGEGAGGQIYAEEFEGLVNSLRRWFAASTSDSLREWVERFMEL
                     TTCETCGGSRLRKESLWFRVAEKNIAELTAMDLNHLAEWFADVEERLSTKQNLIAKDI
                     LKEIRERLRFLLDVGLTYLTLNRPSRTLSGGESQRIRLATQIGSQLQGITYILDEPSI
                     GLHQRDNQRLIHALKNLRDIGNSVLVVEHDKDIMLAADYLVDIGPKAGHHGGRIVAQG
                     RPPALLKLDTLTSSYLNGDRKIVVPAERRKGNGKFLELKGARGNNLQNVDVKLPLGKL
                     IVVTGVSGSGKSTLINETLYPILSKHAYRSRVTPMEYKSIKGLENVDKVIEIDQSPIG
                     RTPRSNPATYCGFFTDIRTLFASVPEAKIRGYTAGRFSFNVKSGRCDVCEGGGMRVIE
                     MNFLPDVYVHCEKCNGRRYNRETLEIRYKGKSISDVLDMTVDDAVDFFQNVPYLYRKI
                     KVLQDVGLGYITLGQSAVTLSGGEAQRVKLGTELGKKDTGKTFYILDEPTTGLHFEDI
                     RHLVEVLDKLVDRGNTVLVIEHNLDVIKVADHIIDLGPEGGDGGGRILFEGTPEELIR
                     NKESFTARFLKEELNGKNHH"
BASE COUNT          371 a          579 c          578 g          474 t
ORIGIN      
        1 aatgaacaac tcatagcggt cgttgatttc gcgcagcact tcaaccgcat ctttcattac
       61 gggcagatgg cggaaaaagc cgggcgcgcg aagccggtcc atgaccatcg gttgatggtg
      121 atcagggaac cccttgaccc aggagccgac aagcattttt tcccagtcca cgtccccgcg
      181 gtgttctttt ctgtaccatt cgatcatctc tcccatcgga tcggcgatga cctcgtccat
      241 gtcaatgatg attctttcca ttcaattctt cttttaggaa cctggcggtg aagctttctt
      301 tgttccggat cagctcctcg ggcgttcctt cgaagaggat cctcccgccg ccatcaccgc
      361 cttcggggcc caggtcaatg atatggtcgg cgaccttgat gacgtccaga ttgtgctcga
      421 tgaccaggac ggtgttgccg cggtcgacga gtttatcgag gacttcgacc aggtgccgga
      481 tgtcctcaaa atgaagcccg gtggtcggct cgtcgaggat ataaaacgtc ttgccggtat
      541 cctttttccc cagttcggtc cccagcttca cccgctgcgc ttccccgccg ctcagggtta
      601 cggcagactg gccgagggta atatagccga ggccgacgtc ctgcagcacc ttgatcttgc
      661 gataaaggta gggcacgttt tgaaagaagt ccacggcgtc gtcgacggtc atgtccagca
      721 cgtcgctgat cgatttgcct ttataacgga tctccagcgt ctcgcggtta tacctgcggc
      781 cattgcattt ctcgcagtga acataaacgt cgggaaggaa attcatttcg atgacgcgca
      841 tcccgccgcc ttcgcagacg tcgcagcggc cgcttttgac gttgaaggaa aacctgccgg
      901 cggtatagcc gcggatcttc gcttccggca cggaggcaaa aagcgttcgg atatcggtaa
      961 agaagccgca gtaggtggcc ggattgctgc ggggcgtgcg gccgatcggc gactggtcga
     1021 tctcgatcac tttgtcgaca ttctccaacc ctttgatgct tttatattcc atgggcgtca
     1081 cccgggaacg gtaggcatgt ttgctgagga tcggataaag ggtctcgttg atcagggtcg
     1141 acttgccgct gccgctaaca cctgtcacaa cgatgagttt cccgagggga agctttacat
     1201 cgacattttg caggttattt cccctggcgc ccttcagctc gaggaatttg ccgtttcctt
     1261 ttcgcctttc cgcagggacg acgatcttcc ggtctccgtt gagatatgaa gaggtcaggg
     1321 tgtccagctt tagcaaggcc gggggtctgc cctgcgccac gatcctgccg ccatggtggc
     1381 cggccttagg cccgatgtcg acgagataat cggcggcaag catgatgtcc ttgtcgtgtt
     1441 ccaccaccag cacactgttg ccgatgtcgc ggaggttctt cagggcgtgg atgaggcgct
     1501 ggttatcgcg ttggtgcagg ccgatactcg gctcgtccag aatatacgtg atcccctgga
     1561 gttgggagcc gatctgcgtc gcgaggcgga tgcgctgtga ttcgccgccg ctgagcgtcc
     1621 gggacgggcg gttgagggta agataggtca ggcccacgtc cagcaggaag cgaaggcgtt
     1681 cccggatctc cttgaggata tccttggcga tgaggttctg cttcgtactg agcctttctt
     1741 cgacgtcggc gaaccactcg gcaaggtgat tgaggtccat cgcggtcagt tctgcgatgt
     1801 tcttctccgc caccctgaac cacaggcttt ccttcctgag ccgggagccg ccgcaggtct
     1861 cgcaggtcgt caactccata aagcgttcca cccattcccg gagcgagtcc gacgtgctgg
     1921 ccgcaaacca gcggcgaagc gaattcacga ggccttcgaa ttcttcggca tagatctggc
     1981 cgccggcgcc ttccccgtcg aa
//
LOCUS       DATGKE010000031         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1545879, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000031 DATGKE010000000
VERSION     DATGKE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545879"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VKU83_00520"
     CDS             <1..726
                     /locus_tag="VKU83_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HLI92054.1"
                     /translation="DLPIADRDPSKPSLWQSADDRRKLISLWRKLAQRYANEPWIGGY
                     DIINEPNWGFSGDTSDPHGTKEKMNAPLRQLMMDITAAIRSVDKHHIIIIEGNGWGNN
                     YAGVLPPWDKNTVLSFHKYWNPNTDEAIRRFLDLRERYNVPLWLGESGENNDEWYRDA
                     VKLVESHDIGWSWWPLKKIGRNQPLEVIMPPAFQKLVDYWVNNGPRPSAAEAQQGLDD
                     LIRNVRVKNNIVHRDVLDALFRP"
     gene            complement(751..1512)
                     /locus_tag="VKU83_00525"
     CDS             complement(751..1512)
                     /locus_tag="VKU83_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92055.1"
                     /translation="MPKPRLYLFAVLFIGAAAGCSLLVNPLTRLWFYTYGTGTPEGAD
                     TLLSPASFLELRPDGSFTRDFGRFEYGTWIKKGHQIILTDQEHRVDSLRFSMPAAGDL
                     KITMTGGYIADFESRPIPAQKAKVDPFSLANNRWRIRPSHKETDREIRERLHNHCQFW
                     ETYFRWALDKGLPAVDVRSTPTPIKIYHNGFTLKPVEDLPPEWVSYFYDTDDCRKANE
                     MIRNIFEHRTIAWANTDNPYKMFLSAFQQMSNYLK"
     gene            1552..2283
                     /locus_tag="VKU83_00530"
     CDS             1552..2283
                     /locus_tag="VKU83_00530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLI92056.1"
                     /translation="MGRTLVIGDIHGGYKALQQVMERAGLGAADRLIFLGDYVDGWSQ
                     SSAVIDYLIDLERGHECVFILGNHDAWCLEWLSGGSLSRDWLFNSGETTIASYRDTSL
                     EGKMRHLEVFKKMRLFYEDDEQRLFIHAGFTSQYGPAYEHIPDACFWDRTLWEMVLAT
                     DTRMSKSDVFYPKRLLLYDEIFIGHTPTTNYDVGTPMHKCNVWNLDTGAGFRGRLTVM
                     DADIKQYWQSDVVQQLYPGEKGRNK"
     gene            complement(2335..3321)
                     /locus_tag="VKU83_00535"
     CDS             complement(2335..3321)
                     /locus_tag="VKU83_00535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLI92057.1"
                     /translation="MKYRMLGNTGIRLSAVGLGCMTMNHGYGEPDDTESVATLEKAVE
                     IGVNFWDTADVYANGRNEELVSKVLVPNRKKIFIATKFGFTTDEQSRLTGFDGSPAHL
                     RVCIENSLRRLKTDVIDLYYAHRIDPKVPVEEMVGAMAELVRHGKVRFLGLSEASATS
                     IRRAHAVHPIAALQSEYSLLSRGVEPEILPLCEQLKITLVPFSPLARGLMTNTLNLAQ
                     LGEKDFRRNLPRYQGEHARNNEHLAKAFSEYALSKGHTAAQLAIAWVLSRGSNVIPIP
                     GTKRRKYLLENAAAADIQLTADDFRDIDALLAKYPNVGDRYNESNYRFTDKT"
     gene            3394..3624
                     /locus_tag="VKU83_00540"
     CDS             3394..3624
                     /locus_tag="VKU83_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92058.1"
                     /translation="MEDYIISVGKVEEWQMTNDVTELEIVFDRAKRVLVGGGVVALVR
                     EQRSGPAYRFEEFRSLEDLEAYKRRVYGLLSR"
BASE COUNT          892 a          930 c         1199 g          938 t
ORIGIN      
        1 gaccttccga tcgcggaccg ggacccgtcc aaaccgtctt tgtggcaaag cgccgacgac
       61 cgccggaagt tgatatcatt gtggcgtaag ctggcccagc gatatgcgaa tgagccgtgg
      121 ataggaggct acgatatcat caatgaacct aactggggtt tttcaggcga tacttccgat
      181 cctcatggca caaaagagaa aatgaacgcg ccgctccggc agctgatgat ggacataacg
      241 gcggcgatcc ggtcagtaga caaacatcat atcattatta tcgaaggcaa cggttggggc
      301 aataattacg cgggcgtact gccgccgtgg gacaagaata cggtgttgag cttccataaa
      361 tactggaacc cgaatacgga tgaagcgatc cggcgattcc tggacttgcg ggaaagatac
      421 aatgtgccgt tatggcttgg cgaatcaggc gagaataatg acgaatggta tcgtgatgcc
      481 gtgaaactcg tcgaaagcca tgacattggc tggtcgtggt ggccgctgaa gaaaataggc
      541 aggaaccagc ccctcgaggt tatcatgccc ccggcctttc aaaagctggt cgattactgg
      601 gtaaataatg gtccacggcc atccgcagcg gaggcacagc aagggctgga tgacctgatc
      661 cgcaatgtca gggtgaagaa caatattgtc caccgggacg tgctggacgc gctgttccgg
      721 ccttagagcg gcgtcaaggg gttggacggg ctacttcagg tagtttgaca tttgctggaa
      781 cgccgagagg aacatcttgt atgggttgtc ggtgttggcc caggcgattg tccggtgttc
      841 aaagatgttc cggatcatct cgtttgcctt ccggcagtca tccgtgtcat aaaaatagct
      901 gacccactcc ggcggaaggt cttcgacagg cttcagcgta aatccgttgt ggtagatctt
      961 gatgggtgtg ggcgtgctcc ttacgtcaac cgcggggagg cctttgtcga gcgcccagcg
     1021 gaaataggtt tcccaaaatt ggcaatggtt atgaagccgt tcgcggattt cgcggtctgt
     1081 ttccttatgg gacggtcgga tccgccagcg attgtttgca agcgaaaacg gatcgacttt
     1141 tgctttttgg gcaggtatgg gccggctttc gaagtcggca atgtatccgc cggtcatggt
     1201 gatcttcaaa tcgccggcgg ctggcatgct gaagcggagg ctgtcgacac ggtgttcctg
     1261 atccgtcagg atgatctgat ggcccttctt gatccaggtg ccgtattcga agcggccgaa
     1321 atcccgggta aaggagccgt cggggcggag ttcgaggaag ctggcagggc tcaataacgt
     1381 atccgctcct tcaggcgtgc cagtaccata agtgtagaac cagagcctcg taagagggtt
     1441 gacgagcagg gagcaaccgg cggcggcgcc gatgaaaaga acggcaaaca ggtataggcg
     1501 gggtttgggc attatgcagg aaatttaggg aattcttttt aaacttgaaa gatgggaaga
     1561 acattggtga tcggggatat ccacggcggc tataaagcat tgcagcaggt tatggagcgt
     1621 gcgggattgg gagccgctga ccggctcatt tttttaggcg attatgtgga tggctggtct
     1681 cagtcatcgg cagtgatcga ttatttgatc gatctggagc gcgggcatga gtgcgtcttc
     1741 atccttggca accacgatgc atggtgcttg gaatggctgt cgggcgggtc gctttcacgc
     1801 gactggttgt tcaacagcgg agagacgacg atcgcgagtt atcgggacac cagccttgaa
     1861 ggaaagatgc ggcacctcga ggtttttaag aagatgcggc ttttttacga ggatgacgaa
     1921 cagcgcttgt tcatccatgc cgggttcacc tcccagtacg gtccagccta cgaacatatt
     1981 cctgatgcct gcttttggga caggacgctt tgggagatgg tgctggccac cgatacccgc
     2041 atgtcgaaaa gcgatgtctt ttatccgaag cggctactgt tatacgatga gatatttata
     2101 ggccataccc cgaccacgaa ctatgacgtc ggcacgccca tgcataaatg taacgtttgg
     2161 aacctggaca ccggggccgg attccgtgga agactgacgg tgatggatgc cgacatcaaa
     2221 caatactggc aaagcgacgt cgtacagcaa ctttacccgg gagaaaaggg caggaacaaa
     2281 tagtccgaac ctacgcccct cggataccac taaccgggta caagaccttg ggtttcaggt
     2341 tttatcggtg aagcgatagt tgctttcatt atagcggtcg ccgacgttgg gatatttggc
     2401 gagcagggca tcgatgtcgc ggaaatcatc cgcggttaac tggatatccg cggcggcggc
     2461 attttccagc aggtattttc ttcttttggt gccggggatc ggaatgacgt tactgccacg
     2521 cgagagcacc catgctatgg caagctgtgc cgccgtgtgg ccttttgaaa gcgcgtattc
     2581 agaaaaggcc ttcgccaggt gctcgttgtt ccttgcatgt tcaccctggt aacggggaag
     2641 gtttcggcga aaatcctttt cgccaagctg cgcgaggttg agggtgttgg tcatgagccc
     2701 gcgggcaagg ggactgaagg gaaccaatgt gatcttaagc tgttcgcaaa gcggaaggat
     2761 ttccggttcc acgccgcggg ataagagcga atattcactt tgaagtgcgg cgatcgggtg
     2821 tacggcatgc gcccttcgga tggatgtggc ggaggcttct gacagcccga ggaagcgcac
     2881 cttgccatgt ctgaccagtt cggccatggc gccaaccatt tcttccaccg gaacctttgg
     2941 gtcaatgcga tgcgcgtaat agagatcgat gacgtcggtc tttaaccgcc gcaggctgtt
     3001 ctcgatacat accctcaggt gtgcgggcga gccgtcaaat ccggtcaggc ggctttgttc
     3061 gtcggtcgtg aagccaaact tggtggcaat gaaaatcttt ttccggttgg gcacgagcac
     3121 cttggagaca agttcttcat ttctgccgtt tgcatagacg tcagccgtgt cccagaaatt
     3181 gacgccgatc tcaaccgcct tttcgagcgt ggcgactgac tctgtatcat ccggctcgcc
     3241 gtagccgtga ttcatggtca tgcagcccag gcccacggcc gagagtcgga taccggtgtt
     3301 tccaagcatt ctgtatttca tttcgcaaag gtccccaatt tgccttgaag ggccggagaa
     3361 tttcgggtta attttgtggc aggagaatga gttatggaag attatattat ttcggtgggc
     3421 aaggtggagg agtggcaaat gacaaatgac gttaccgaat tggagattgt ttttgaccgc
     3481 gcgaagcggg tgttggtcgg tggcggcgtg gtggccctgg tcagggagca gcgttccggg
     3541 cctgcgtacc ggtttgagga gttccgtagc cttgaggatc tggaggcgta caaaaggagg
     3601 gtgtatgggc tcctttcccg atgatgccgc ccttcgggaa ccggaacccg gtgggggaaa
     3661 ggcggcaggt gcggagggac cggatcggtc ttaaaacaaa agaggccgtc gatcaagaca
     3721 gcctcctctg actttcgtca gcagattatt ttttggagca acccggttaa gcgagattaa
     3781 ccgaaggttt ccatgattta tctgtagata gcatgaatac ctcctttttt ttggtgaatg
     3841 ataggtataa agttacggaa aaatcaggat aattgacgat gcagggccca ttttctaagt
     3901 ttgcctacga gctcttccag tttgacaggc ttgccgacaa aatcattcat ccccatttg
//
LOCUS       DATGKE010000032         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_717513, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000032 DATGKE010000000
VERSION     DATGKE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717513"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VKU83_00545"
     CDS             complement(<1..774)
                     /locus_tag="VKU83_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HLI92059.1"
                     /translation="MKKTIISFVLFLAVSVIAFRQADAQEFSVGVSIYVGPPVIPVYT
                     QPPCPVDGFLWVPGYWAWDDDYGYYWVPGYWAEPPEIGFLWTPGYWGCQDGIYAWHAG
                     YWGPEIGFYGGINYGCGYYGSGYYGGRWEGRYFRYNTAVTNVNTTIIHNTYIDRTVIR
                     NTTVNRVSYNGGPGGINARPTPQQEAAARGRHVPATQAQISQQHTASRDRNQFASFNH
                     GHPSNVAVVRPTAYHPASDRAGLPRATAFNRPGGSAIRPA"
     gene            911..2020
                     /locus_tag="VKU83_00550"
     CDS             911..2020
                     /locus_tag="VKU83_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI92060.1"
                     /translation="MTIKHLVGIVSRNRKKFIPALLASLFATAVHGQKVLRVGVAGLT
                     HDHVHNIMHQFRSGEVIIAGIAETDPALIARYKKQYQLPDSLFYSSLAALIDHAHPDA
                     VLAYNAISEHLGVVEACAPKGISVMVEKPLATTVGDADRIAALAKQYHIHVLTNYETT
                     WYNSNQQIYEMVNGQHAIGQTRKMIAHDGHQGPREIGCSEDFLKWLTDPVGNGGGASR
                     DFGCYGANLMTWLMHGQPPVAVMATLRHYRPEVYPKVDDDATIVLEYPGATGIIEASW
                     NWPFGIKDWEVFGSTGYLHALNDKTLEERTGSSYDTIPVHPAAYRDNISYLADVLSGK
                     TNPGNDLSSLANNLVVVRILDAATRSAREGKRVTL"
     gene            complement(2029..>2665)
                     /locus_tag="VKU83_00555"
     CDS             complement(2029..>2665)
                     /locus_tag="VKU83_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92061.1"
                     /translation="VMMVKRQGFWDRSVASGAADTASARTANGLDSGKAAAGPARGDS
                     GTVGSGGTGAVAGAGGGLPPLADSVFTRLYRPYMRGDDPIEVRNYYVDYRSGNYAAVL
                     AVGDSTGLGIGQRKVVIRNYMRLYRGLSYLATGDAKDAVPELEGVVLRTKPGDILYET
                     AQWYLALAWVKRTDVDPVDARNNALGLAREVSHSYSRYNGPARELVKELTP"
BASE COUNT          602 a          847 c          664 g          552 t
ORIGIN      
        1 cgccggacgg atcgcgctcc cgcccggccg gttaaatgcc gtcgccctgg gcaggcctgc
       61 cctgtcactt gccggatggt acgccgtcgg ccgcactacc gccacgttcg acgggtggcc
      121 atggttaaag gaagcgaact gattgcggtc gcggctggcg gtatgttgtt gtgaaatctg
      181 cgcctgagtg gccggcacgt ggcggcccct ggccgctgcc tcctgctgcg gcgtcggcct
      241 ggcattgatg ccgccggggc cgccgttata gctcacgcga ttgacggtgg tgttacgaat
      301 gacggtcctg tcgatatacg tgttatggat aatggtggtg ttgacattgg tcaccgctgt
      361 attataacgg aaatacctac cctcccatct accgccgtaa taccctgatc cgtaataacc
      421 acatccgtag ttgataccgc cgtagaagcc gatttccgga ccccagtaac ccgcatgcca
      481 tgcgtatatg ccatcctgac aaccccaata cccgggcgtc cataaaaagc ctatttccgg
      541 cggctccgcc caataacccg gcacccagta ataaccgtag tcatcatccc atgcccaata
      601 tcccggcacc cagagaaaac cgtcgacagg acaaggcggc tgcgtgtaca ccggaataac
      661 aggaggtccg acgtaaatgc tcacgccgac gctgaattct tgggcgtcag cctgcctgaa
      721 cgctataacg ctaacagcca ggaataaaac gaatgaaata atggtcttct tcataaattt
      781 tggttttgac ccaggaaact acatttgttc ttctcttgct taatgcaaga ttgtagccaa
      841 agcgttacga aaaaacgcca tatcttgcca aagctttgtt aattgaaccg ttgtttcgca
      901 aatcaatgtt atgactatca aacacttggt cggtatcgtt agccggaaca gaaaaaagtt
      961 tattcccgca ttgcttgcgt ccctgtttgc tacggccgtt catgggcaaa aagttctacg
     1021 tgtcggagtt gccggcctga cgcacgatca cgttcacaat atcatgcatc aattcagatc
     1081 gggtgaagtc atcatagccg gcatcgcaga aacggacccg gcgttgatcg cccggtataa
     1141 aaagcagtat cagcttcccg attctttatt ttattcgagc ctcgcggccc tcatcgatca
     1201 tgcgcacccc gacgccgtgc tcgcctataa tgcgatctca gaacacctcg gcgtcgtcga
     1261 ggcctgcgcg ccgaaaggca tttccgtcat ggttgagaaa cccctggcta cgacggttgg
     1321 ggacgccgac cgaatcgccg ccctcgccaa acaataccat atccacgtcc ttacaaacta
     1381 tgagaccacc tggtacaaca gcaaccagca gatctacgaa atggtaaacg ggcagcacgc
     1441 tattggccaa acccgcaaga tgatcgccca tgacggccac cagggaccaa gagagatcgg
     1501 ctgcagcgag gactttttga aatggttaac ggaccccgtt ggcaacggcg gcggcgcaag
     1561 ccgtgatttt ggctgctacg gcgcaaacct gatgacctgg ctcatgcatg gccaaccgcc
     1621 cgtcgcggtc atggcaaccc tccgccatta ccggcccgaa gtctacccga aggtcgatga
     1681 tgacgccacg atcgttctgg aatatcccgg ggccaccggc attattgaag cttcctggaa
     1741 ctggccgttt ggaattaaag attgggaggt tttcggaagc accggctacc tgcacgcgct
     1801 gaatgacaaa acgctggaag aacggacagg gtcgtcctac gataccatcc cggtgcatcc
     1861 cgccgcgtat cgtgacaaca tcagctatct ggccgatgtc ctcagcggaa agaccaaccc
     1921 cggcaacgac ctctcctccc tcgccaataa cctcgtcgtc gttcgcattc tcgatgccgc
     1981 cacccgctcc gccagggaag gcaaacgggt aacgttgtaa acggcatcct agggcgtcaa
     2041 ttccttaacc aactcgcgcg ccggcccatt ataacgggaa tagctatgcg aaacttcccg
     2101 cgccaggccc aacgcattat tcctcgcgtc aacgggatcc acgtcagtcc tttttaccca
     2161 ggctaaagcc aaataccact gcgccgtttc atataaaatg tcacccggct ttgtcctcag
     2221 gacaacaccc tcgagttccg ggaccgcatc ttttgcatcg ccggtcgcca ggtaggacaa
     2281 accccggtac agccgcatat agttcctgat caccaccttg cgctggccga tgcccaggcc
     2341 ggtcgaatcc cctaccgcca gcacggccgc gtaattgccg ctacggtagt ccacgtaata
     2401 attcctgact tcaataggat cgtcgccgcg catatagggc ctgtatagcc gggtgaagac
     2461 actatccgcc agaggcggta agcccccccc tgcaccggcg acagcacccg tcccgcctga
     2521 cccgaccgtt ccgctgtctc cacgcgccgg ccccgctgcc gccttccccg agtccaaccc
     2581 gttcgccgtc ctcgcgcttg cagtatccgc tgcgccggaa gctaccgaac ggtcccaaaa
     2641 tccctggcgc ttcaccatca tcacc
//
LOCUS       DATGKE010000033         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_95341, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000033 DATGKE010000000
VERSION     DATGKE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95341"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..376)
                     /locus_tag="VKU83_00560"
     tRNA            complement(302..376)
                     /locus_tag="VKU83_00560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(339..341),aa:Val,seq:tac)
     gene            complement(475..2370)
                     /locus_tag="VKU83_00565"
     CDS             complement(475..2370)
                     /locus_tag="VKU83_00565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI92062.1"
                     /translation="MAQAPAAYNPHALFAPGFYRDDHLATRSPRGTPSAAYWQNRADY
                     QLTAVLDTVANTIRGTDLIRYTNNSPDSLRSLWLYLEQQTYRPDARSNFYTGVAPDRH
                     TAGFQLESVKIRQGGEPMDADTVISDTRLQVRLPRPLAGHGGQITIEIRYHYTVPADF
                     GNRTDFFSTRNGKVYEIAQWYPRMCVYDDRQGWNTLPFLGSGEFYCEYGNFDYRVTVP
                     WDMIVAGSGELQNPQEVLTAAQRSRLDAARASDATVMIRSAAEVGEASSRPVHSGTLT
                     WHFKMSNTRDVVFGASRAYIWDAARVNLPAGKKCLAMSVYPVESAGPGAWGRATEYLK
                     SSIEYFSAQWFPYPYPVAVNEAGIAGGMEYPGIVFDGYTDAGKELYWVTAHEIGHNWF
                     PMIVGSDERTYGWMDEGFNTFIDVYASEAFNHGEYAPKRDGEYAPGGGNPVDEILPLL
                     KDPAAPVMLTRADAIPEKYRHPIVYFKPALGLVLLREQILGPKRFDWAFRQYIRSWAY
                     KHPGPYDFFRTMENEGGEDLCWFWREWFFHNWSLDLAVQAVHAFHGGYDITVANLDRM
                     AMPATLELTWKDGTKQRVSLPVETWLQSGLRTLHFSSDQPLTGVIIDPDHVLPDDDRS
                     NNSWKPS"
     gene            complement(2452..2769)
                     /locus_tag="VKU83_00570"
     CDS             complement(2452..2769)
                     /locus_tag="VKU83_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92063.1"
                     /translation="MVRMSTSMTMRKFQVTIRFDLNDEFAALVPPHRTYINRLIEQGI
                     VDHYVVTMETQRVWITLSAENKKSVERYLSKSPLYKYWTYEIDELFMVDGLHYRLPVV
                     QLN"
     gene            complement(2795..>3475)
                     /locus_tag="VKU83_00575"
     CDS             complement(2795..>3475)
                     /locus_tag="VKU83_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLI92064.1"
                     /translation="EIVLTGVNLGDFGGNGEDFFGLIRELDNMEGIDRFRISSIEPNL
                     LTPEIIEWVASSRRFMPHFHIPLQSGSNPILGLMRRRYRRELYAERVRLIRNVMPHAA
                     IGVDVIAGHPGETAGHFSETIEFLESLDVSYFHVFTYSERDNTQALELGPVVPMAVRA
                     ERNKTLRNLSYRKLQAFIQRHRGQTRPVLLEGHVTTPVMEGYTDNYIKVTTPYRAEWA
                     NRIIDWQL"
BASE COUNT          773 a          981 c          979 g          742 t
ORIGIN      
        1 tttttctcat agcatctcgg tcaggacgaa cataaaatcg gctggtttct ctcaagtcac
       61 cacaaacgta agtcacaccc ttagccttcg catctatatg agctttacgt tcggaaagct
      121 gaaggtcgaa agggaaggaa agcaaaagaa agccattacc aatgatgatc tgaagtaatc
      181 ttgcactgct ttcgccggtg tgaagggttg gaaatgttgt gcagacaacg aaagggagag
      241 ttgtcctcaa caactggcaa agtgcgacaa tgggtgacct cgattaatcg aagtctttct
      301 gtgggagctg agggtctcga acccccgacc ctctgcttgt aaggcagatg ctctgaacca
      361 actgagctaa gctcccatct tccggcgaac caaggaggtt ccatcgaaag gagggcaaag
      421 ataagggcaa aatcgttgcg ccccaatttt tatgccccat tttccagccg gccatcaact
      481 cggtttccag ctattattgc ttcggtcatc atccggcaga acatggtccg gatcgatgat
      541 gacgcccgtc aaaggctggt ccgaagaaaa atggagggtt cgtaagccgc tttgcaacca
      601 ggtctcaacc ggcagcgaca cccgctgttt cgtcccgtct ttccaggtca gttccagcgt
      661 tgccggcatg gccatccggt cgaggttggc gaccgtaata tcatatccgc cgtggaaagc
      721 gtgtacggcc tgcaccgcca ggtcaaggct ccagttgtgg aagaaccact cccgccagaa
      781 ccagcagagg tcttcaccgc cctcattttc cattgtccgg aagaagtcgt agggccccgg
      841 atgtttatac gcccaggagc ggatatactg ccggaatgcc caatcgaaac gcttcgggcc
      901 caggatctgc tcgcgcagga ggaccaggcc aagcgccggc ttaaaataga cgatggggtg
      961 gcgatacttc tccgggatgg cgtccgcgcg ggtcagcatc acgggcgccg ccggatcctt
     1021 gagcaggggc aggatttcgt cgacagggtt gccgcctccg ggcgcatatt cgccatcccg
     1081 ttttggggca tattctccat ggttgaaggc ttcggaagca taaacgtcga tgaaagtatt
     1141 gaagccttcg tccatccatc cataggttcg ctcgtcgctg ccaacgatca tggggaacca
     1201 gttatggccg atctcatgcg ccgtgaccca atacagctcc tttccggcat ccgtatagcc
     1261 gtcaaacaca atacccggat attccatccc accggcgatc ccggcttcat tgactgccac
     1321 agggtaaggg taggggaacc actgggcgga aaagtactcg atagagcttt tcaggtattc
     1381 ggtagcgcgg ccccaggcac cgggccccgc gctctccacc ggatacaccg acatcgccag
     1441 gcattttttc ccggcgggca ggttgaccct cgcagcgtcc cagatatagg cccggcttgc
     1501 gccaaaaacg acatcccggg tattggacat tttaaaatgc caggtaaggg tgccggaatg
     1561 gacagggcgg ctcgacgctt cgccaacttc cgcggcactc cggatcatga ccgtcgcatc
     1621 gctggcgcgg gccgcgtcca gccttgaccg ctgggcagcc gtcagcacct cttgcggatt
     1681 ctgcagctcg cccgatccgg cgacgatcat gtcccagggt acggtgacgc ggtagtcaaa
     1741 attgccatat tcacaataga attccccgct gcccaggaag ggcagtgtgt tccagccctg
     1801 gcgatcgtca tatacgcaca tgcgcggata ccattgcgca atttcgtaaa ccttcccatt
     1861 tcgggtggaa aagaagtctg tccggttgcc gaaatcagct gggaccgtat aatgatagcg
     1921 gatctcgatc gtgatctgcc cgccatgtcc ggccagcggg cgcggcagcc ggacctgaag
     1981 cctcgtatcg ctgatcaccg tatcggcgtc catcggttcg ccaccctggc ggatctttac
     2041 cgactccagc tgaaagccag cggtgtgccg gtcgggggca accccggtgt agaaattgga
     2101 cctggcatcc gggcgatagg tctgctgctc gagataaagc cagagagagc gcaggctgtc
     2161 cgggctgtta ttggtatacc ggattagatc cgtgccccgg atagtgttgg cgacggtgtc
     2221 cagtactgct gtcagttgat agtcggcccg gttctgccag taggcggccg aaggcgttcc
     2281 gcgcggggaa cgtgttgcaa gatggtcatc gcggtaaaac ccgggcgcaa acaatgcatg
     2341 gggattatag gcagcgggcg cctgtgccat gcaggcggca ttaaccaata gcccaataat
     2401 taaaagcgaa ggaaaggaaa gtcttgccat ggtgcggcga aaatagccaa actaattcag
     2461 ctgcacaaca ggaagccgat agtgcagccc gtccaccatg aagagttcat cgatctcgta
     2521 cgtccagtat ttgtacagcg gagactttga caggtaacgc tcgaccgatt ttttgttttc
     2581 agctgaaagc gtgatccata cacgctgggt ctccatcgtc accacgtagt gatcgacaat
     2641 gccttgttcg atcagccggt ttatataagt ccggtgaggt ggaaccagcg ctgcgaattc
     2701 atcgttcaag tcgaacctta tagtgacctg gaatttcctc atcgtcatgc ttgtactcat
     2761 cctgaccatt cggcagcaat gttcgtgccg cggcctacag ctgccagtca atgatccggt
     2821 tggcccattc cgcgcggtag ggcgtcgtaa ccttgatgta attgtcggta tagccttcca
     2881 tgacgggagt ggtaacatgt ccttccagca ggaccggacg ggtctgcccg cggtgccgct
     2941 ggataaaagc ctgcagctta cggtaggaca ggttccgcag cgtcttgttg cgttccgccc
     3001 ggacggccat cgggacgacc ggccctagct ccagagcttg ggtgttgtcc cgctcggaat
     3061 acgtaaaaac gtggaaatag gaaacgtcga gagactccag gaattcgatc gtttcgctaa
     3121 aatgtccggc ggtttcgccg gggtggcccg cgatgacgtc cacgccaatc gccgcgtgcg
     3181 gcatgacatt ccggatcagc cgcacccgtt ctgcataaag ctcccgccgg tacctgcggc
     3241 gcatcaaacc caggatggga ttactgccgc tttgcagcgg aatatgaaaa tggggcataa
     3301 aacgccggct ggatgccacc cattcgatga tctccggcgt cagcagattg ggctcgatcg
     3361 aagaaatccg gaagcggtca atcccttcca tgttgtcaag ctcgcggatg agcccgaaaa
     3421 agtcttcgcc gttcccgcca aaatctccca ggttgacgcc ggtgagtacg atctc
//
LOCUS       DATGKE010000034         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1133671, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000034 DATGKE010000000
VERSION     DATGKE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133671"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VKU83_00580"
     CDS             complement(<1..1523)
                     /locus_tag="VKU83_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HLI92065.1"
                     /translation="MDHQKHMNNLSTAGLLVALGIIYGDIGTSPLYTFQTILTEGGEA
                     NRLLVLGAISCVFWTLTLQTTFKYVLITLQADNHGEGGVFSLYALVRRYSKTLFYIAI
                     IGAGTLLADGIITPPITITSAVEGLSMVKGLQQHIMPGNTLTVEIVMVILLLLFIFQR
                     FGTKIVGGSFGPVMFCWFFMMGSVGLLQVVRNPIVLEALSPWYGIRLLTMHHNGFWLL
                     GAIFLCTTGAEALYSDLGHCGRRNIRVSWIFVKTTLLLNYLGQGAWVLLQHENSIGTV
                     NPFFAIVPQGFLLPAIVLATAASIIASQALISGSFTLINEAMSLGFWPRINVKYPSNI
                     RGQIYIPSINWILFIGCALVLLYFRTSEAMTAAYGFSITIAMLMTTFLMFYYLRYVRR
                     MAFWIVGAILTVFVCVETSFFIANAVKIVKRLFFLVFEIGLISTMYIWFNARRITQRF
                     LTFVNLQDYIPMLEALSRDPSVPKYSSHLVYFTRSAYSKLIEKRILDSIFDNIEKKA"
     gene            1814..2641
                     /locus_tag="VKU83_00585"
     CDS             1814..2641
                     /locus_tag="VKU83_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92066.1"
                     /translation="MDAATDDVNNSLNSSATFSGAASYNGGVVTDGGGGSMNLDLGIC
                     DATVTVDTANGLRQIVITYNGTNCRGNRSRTGVVVVSIPVGTRWRDKGAVVTVNIENL
                     KITRLIDNKSITLNGTYTYTNVTGGLLKDLATLGTITHTITADSVSIEFADSATRIWS
                     VSKQRDFTYNDGIVETTTGTHSDGTNTDVAIWGINRFGNSFETLITQPLVVEQSCDFR
                     LTSGQTETIRPALTLTITYGLDSNGDPTSCPGSGYYYFKAVWIIKKNGKTYTWIAPY"
     gene            3024..>3533
                     /locus_tag="VKU83_00590"
     CDS             3024..>3533
                     /locus_tag="VKU83_00590"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLI92067.1"
                     /translation="MPVSKRRCAWPGEDPLYQKYHDEEWGKHCTDERTLFEFLILESA
                     QAGLSWITILRRRGNYRKAFANFDAKKVAAFTEADVRRLLADEGIIRNKLKIRAAIDT
                     ARLFLQVQKEFGSFYKYLYGFMPGNKPIVNNSPKLIASSPISDAISKDMKKRGFKFFG
                     TTICYAYMQA"
BASE COUNT          916 a          989 c          864 g          764 t
ORIGIN      
        1 tccgccttct tctcgatgtt gtcaaaaatg gaatccagga tccgcttctc aatcagcttc
       61 gaataagccg accgcgtgaa atagaccagg tgcgaactgt atttcggcac cgaaggatcg
      121 cggctgagcg cctccagcat ggggatataa tcctggaggt tcacaaaggt caggaaccgt
      181 tgtgtgatcc gccgggcgtt aaaccagata tacatcgtgg agatcaaacc gatctcgaag
      241 acgagaaaga agagtcgctt cacaatcttg accgcattag cgataaagaa gctcgtctcc
      301 acgcacacaa agacggtcag gatcgccccc acgatccaga acgccatccg gcggacatat
      361 cttaagtaat aaaacatcaa aaaggtcgtc atcagcatgg cgatcgtaat agagaacccg
      421 tacgccgccg tcattgcctc cgaggtccgg aaataaagga gcaccagcgc gcagccgatg
      481 aagaggatcc agttgatgct ggggatatag atctgtccgc ggatattgga gggatatttg
      541 acattgatgc gtggccagaa gccaaggctc atggcttcgt tgatcagggt gaaagatcca
      601 ctgatcaggg cctggctggc aatgatcgaa gcagccgtgg caagcacgat cgcgggcagc
      661 aggaagccct gcggaacgat tgcaaaaaag ggattgaccg taccgatgct gttctcatgc
      721 tgcagcagca cccacgctcc ctgccccagg tagttcagga gcagcgtcgt tttcacaaag
      781 atccaactca cgcggatatt ccggcgcccg caatggccga ggtctgaata tagggcctcc
      841 gcaccggttg tacacaaaaa gatcgccccc aataaccaga agccgttgtg atgcatggtc
      901 aggagccgga tcccatacca ggggctcagc gcctccagca cgatcgggtt ccgcactacc
      961 tgcaataaac ccaccgaccc catcataaag aaccagcaaa acatcacggg gccgaaggaa
     1021 ccgccgacta ttttggtacc aaaacgttgg aaaatgaaga gcaacaacag gatcaccatg
     1081 acgatctcga ccgtgagggt atttcccggc atgatatgtt gttgcagccc cttcaccata
     1141 ctcaatccct ccaccgccga agtgatcgtg atcggcggcg tgatgatccc gtctgccagc
     1201 aatgtccccg cgccgatgat cgcgatataa aagagggttt tgctatagcg tcgcaccagt
     1261 gcatacaagg aaaagacccc gccttctccg tggttgtctg cctgtagggt tatcagcacg
     1321 tatttaaagg tcgtttgcag cgtgagggtc cagaagacgc aggaaatagc ccccagcacg
     1381 agcaaccggt tggcttcccc accctccgta agaatggtct gaaaagtgta cagcggcgaa
     1441 gtcccgatat ctccataaat gatgcccaac gctaccagta atcccgcggt ggacaggtta
     1501 ttcatgtgct tctgatggtc cattatcgtt tgcccggcaa tttttaatcc attttctgtg
     1561 gcggcctaag acccgcaaaa atccgaatag tttaaccatt ctacctaatt ttttataaaa
     1621 taaaaaatat atatgagcgc ccttaaactt cacccattca acccggatca gaaccgtaaa
     1681 tcaactgaaa ctgttaaaac acacttcatg aaaacaaaac agtaccggta acggctccac
     1741 tccatcaggc gatggcacca gtacttcaga ggtccaaacc cagtcgaatg acataacgca
     1801 agtctccagc gaattggatg ccgccaccga cgacgtcaac aacagtctta acagctccgc
     1861 aacctttagc ggcgcggcca gctacaacgg cggcgtcgtc acggatggcg gcggcgggtc
     1921 catgaacctg gacctcggca tctgcgacgc cacggtaaca gtggataccg caaacgggct
     1981 tcgccagatc gtgatcacct acaatggcac gaattgccgg ggcaaccgga gccgaaccgg
     2041 cgtcgtcgtc gtttccattc ccgtcggcac ccgctggcgc gacaaaggcg cggtcgtgac
     2101 cgtcaacatt gaaaacctca agatcacccg cctcatcgac aacaaaagca tcaccctgaa
     2161 cgggacctat acctacacca acgtgaccgg cgggttgttg aaggacctcg ccaccctcgg
     2221 cacgattacc cacacgatca ccgctgacag cgtaagtatc gagttcgccg acagcgctac
     2281 ccgcatctgg agcgtttcca agcagcgcga cttcacttac aacgatggga tcgtcgaaac
     2341 tacgaccggc actcactctg acggcactaa taccgacgtc gccatctggg ggatcaaccg
     2401 attcggaaac tcattcgaga ccctgatcac tcaacccctg gtcgttgagc agtcctgcga
     2461 cttcaggctg accagcggtc aaacggagac catccggcct gcccttaccc tgaccattac
     2521 ctacgggctt gattctaatg gtgatccgac cagctgcccc ggatcgggat actattattt
     2581 caaggcagtt tggataatta agaaaaacgg caaaacctat acttggatcg caccttactg
     2641 atatgttccg taccgatttt cgatcttgac ccgttataaa agaggttgtc tcctgtggct
     2701 tggaggcgac ctctttattc ccaaaacttc ccagtgggga atctttttcc cgtggcctat
     2761 ttcccaatct tgagtaattt ctgcgcgctt gtcccgtatc cttctcgaat ttatgacact
     2821 acacttttat tgtttattat atatattata agtttatcaa aggcgcacct tttgccctag
     2881 taaagtgcga ctcttaatag gatattggaa tttagcaacg gcctggattt tcatccgggc
     2941 ttcttttttt ggacgccggc taagcccaaa tggttttggt tccatagcgg ccgggcagtt
     3001 cccggccgta tatttaccaa aaaatgcctg tttccaagcg acgctgcgcg tggccggggg
     3061 aagaccccct gtaccaaaaa tatcatgacg aagaatgggg caagcactgc accgacgaac
     3121 ggaccttgtt cgaattcctg atcctcgaat cggcacaggc gggcctcagt tggatcacca
     3181 tccttcgccg gcgtgggaat taccgaaagg ccttcgcgaa tttcgacgcg aaaaaggtcg
     3241 cggcgttcac ggaagccgat gtgcggcggc tactcgcaga cgagggcatt atccgcaaca
     3301 agttgaagat ccgcgcggcg atcgacacag cccggttatt cctgcaggtc cagaaggaat
     3361 tcgggagctt ctacaagtat ttgtatggtt ttatgcccgg caacaaacct attgtcaata
     3421 acagcccgaa gctcatcgcc agctcaccca tctccgatgc gattagcaag gacatgaaaa
     3481 agcggggttt taaatttttt ggtacgacaa tctgttatgc ctatatgcag gcc
//
LOCUS       DATGKE010000035         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1340036, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000035 DATGKE010000000
VERSION     DATGKE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340036"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1154)
                     /locus_tag="VKU83_00595"
     CDS             complement(372..1154)
                     /locus_tag="VKU83_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92068.1"
                     /translation="MNGLPSGQFVDYLIIGAITSACAVILDLILKSKTVRKRSRIMVI
                     IILVSIAAFTVFVKIRTETNTAVGTNPRSSSTLSPAIISSSPLNHKPEQKINRDLLTA
                     IYVAPPPKKQRKSGQWAVIIVDPKNNQYYPQLTDAVSSAIAQAGQSTVAMFRPAATKG
                     PGFEIFFAADPSLSRRLHEYCDQIVLGEVSSIMQDNSVAPGLLRLSLTINLKIISTGS
                     GEVQHEIHASAMGAGYEADEARSNAEENLAATLGTTLQSAIK"
     gene            complement(1173..2123)
                     /locus_tag="VKU83_00600"
     CDS             complement(1173..2123)
                     /locus_tag="VKU83_00600"
                     /inference="COORDINATES: protein motif:HMM:NF036774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HLI92069.1"
                     /translation="MLERYPCTQRSALYFFLLMVLLASANCLGQQNDLNQVASSLARD
                     INAGNRHIVTVADFTDLQGKVTELGRFISEELSTRLVIDAKNFNVVERIQLAAILKEH
                     QISISGLVDPATIRKLGQFAGVDAIVTGTFVPFSDSVRLTAKVLDVSTARVMAVSTAE
                     LPRTKAIDDLLSRGIATGSGPNNASSGGTTHPSNPTKIFSVEQNEFMILLKTCSPRGD
                     RVVCSGSITNKANRARHFQIMNGSTSAIDNNGNSYAANQADIGQGVATDLMPDLPTNF
                     HLDFPGVDSAATSLNVIFAYLVDNINFTKALFRNLPLPAK"
BASE COUNT          561 a          513 c          565 g          594 t
ORIGIN      
        1 tcccgcaggc tcggtggcag cagcaaatcc tcatccgggt agtacggacg gtaacttttg
       61 cccatgcccc attatgatgc accaccttca caacgccaag ccaaaatgca ggtcatcgtg
      121 aattctcgga cagactccta gcatccaacc gcctggaact cccttaaatt attttgagga
      181 ggaatcaagt ttataacaca gtaatagtag gccgacaaac cttaattgga tgaagtaatt
      241 tttgaaggtc tataggtcgc atggccatgg gttcgcccat ggcagcgcgg ccggcgcatc
      301 atggccggcc gcgcgggtgt cgtgatcctt gaattggcgt cagcatcggg ttgtgattat
      361 ctccggcgca gctatttgat tgcgctttgc agtgtggtgc ccagagtggc ggccaagttt
      421 tcctctgcgt tgcttcgtgc ttcatcagct tcataacctg ctcccatcgc agaagcatgg
      481 atttcatgtt gcacttcgcc ggagccggta gagatgattt ttaaattaat agtaagggag
      541 agtcttagca atcctggagc aacagaatta tcctgcatga tggaagagac ttcacctaag
      601 acaatttgat cgcaatattc atgtaggcgt cgggataggc tgggatcagc cgcaaaaaat
      661 atttcgaacc caggtccttt cgtggcggct ggacggaaca tcgcgactgt ggattgcccg
      721 gcttgtgcaa ttgctgatga aaccgcatct gttaactgag gatagtattg attgtttttt
      781 gggtccacaa tgatcacagc ccattgcccg cttttgcgct gtttctttgg gggaggcgca
      841 acatatatcg cggtcaataa atcccgattg attttctgct ccggcttatg gttcagaggt
      901 gatgaggata ttatagcggg agatagagtc gaagacgatc ggggattggt tccgacagcg
      961 gtgtttgttt ctgtgcgtat ttttacaaac acggtgaatg cggcaatgga aactaaaata
     1021 ataatcacca taatccgcga tcttttcctg actgtcttac tctttaaaat taagtccaaa
     1081 ataaccgcgc aggcgctagt gatggcgccg ataatcaggt aatcgacaaa ttgtccagaa
     1141 ggaaggccat tcatttgatc tcgtcctctt ttttatttcg caggcagcgg caggttgcgg
     1201 aacagagctt tagtgaagtt tatattatca actaaataag caaaaatgac attgaggctt
     1261 gtggctgcgc tgtcaacacc tggaaaatca agatggaagt ttgtgggcag atccggcata
     1321 agatcggttg ccaccccttg ccctatatcc gcctgattcg ctgcataaga gttgccgttg
     1381 ttgtcaatgg cgctcgtact gccattcatg atttggaaat gcctggccct atttgcttta
     1441 tttgtgattg aacctgaaca aacaacgcgg tctccgcggg gagagcaagt cttgagcagg
     1501 atcatgaatt cattctgttc aacggaaaaa atctttgttg gattagaagg atgtgtcgtc
     1561 ccgccagacg aagcattatt tggtccgctg ccggtagcaa tgcccctaga gagcagatcg
     1621 tcgatggcct tggttcgggg caattccgcc gttgaaactg ccatgaccct tgcggttgag
     1681 acatcgagca ctttcgcggt gagccgtact gaatcggaga aaggcacaaa ggtgcctgtc
     1741 acaatggcat cgacaccggc aaattggccg agctttcgaa tcgtggccgg gtccaccaaa
     1801 ccggaaatgc tgatctggtg ttcttttaga atggcagcta gctggatcct ttccactaca
     1861 ttgaaatttt ttgcgtcgat aaccaacctg gtggacagct cttcggagat aaagcggcct
     1921 aattccgtca ctttcccttg cagatccgtg aaatcggcga ctgtaacgat atgtctattc
     1981 cccgcattga tatcgcgggc cagcgacgaa gcaacctggt tcagatcatt ttgctggcca
     2041 aggcaattcg ccgaagcgag aagaaccatg agcaagaaaa aataaagtgc ggatctttgc
     2101 gtgcaaggat agcgctctag cattcatgcc tcccagaata ttttatgtga aacgtattgt
     2161 gctgcccatg tttttatatc ctaatttcgc caaaacgtga ataatctcag gtggctttga
     2221 aaaaaacaga aga
//
LOCUS       DATGKE010000036         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_503897, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000036 DATGKE010000000
VERSION     DATGKE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503897"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VKU83_00605"
     CDS             complement(<1..811)
                     /locus_tag="VKU83_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92070.1"
                     /translation="MIGIFRQKNPANIVVLLCYALVLKFPLFLHPVAPALHPEDNYLY
                     RVILKGLNTVFDQLPILFSLLTFLLIFTQAALFNSIFNHHKVLPKPTYLPGMSYILIT
                     SLLPDWGHFSAPLLINSVMVLVWYRMLDLYNSPRPGSAIFNIGLWTGIVSLLYIPAVA
                     FLLLVLFGLLTMRPFRVREWIVGLLGFSFPYYFLFLILYLSGHWAWSDIVPKIVFNVP
                     AFPASIWVSLGMAWLVIPFMIGGYFVQKNLSKFLIQIRKSWSLLLLYLLVAV"
     gene            complement(828..>2081)
                     /locus_tag="VKU83_00610"
     CDS             complement(828..>2081)
                     /locus_tag="VKU83_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92071.1"
                     /translation="FVAYEDYFLLIYEYQKKSIVHIAAVKLGGQAQLIGKPMELDTTQ
                     VGFASNNKIYTVIVSEDKQRIMLYKINSHDPRNFLFTTFLYDSELKLVDRHRLFLPME
                     DRNELFTDFLVDNEGTFVFAKYLRSGSNDYVSRVSVVTKGPTADTFSVHDIGAGDRIL
                     DEIKIKVDNNNNRYIISGFYYKQRRGNIEGLYTVFWDKATNSKQAEKVTVFSDELRAL
                     AKSSDATIKTAFNDFFIKQIVTKRDGGYLLVAESLYTTSRGSMFNRWDYMNPIYGYGP
                     GFYSPYYNPYYNPWARNYGGYGTRFHAENIMVLSYDRESNLQWSNIIPKSQFDDESDN
                     MISNEMVNTGRELNFLFNLYERRNMLLNDQSISPEGRITRYPTLHNLDRGYEFMPRYG
                     KQVSSWESIIPCMYRNYLCFAKVDF"
BASE COUNT          553 a          508 c          560 g          460 t
ORIGIN      
        1 cgaccgccac aaggagatag agcagcagca ggctccagct tttccggatc tggatgagaa
       61 atttgctcag gttcttttgg acaaaatagc cgccgatcat gaagggaatg accagccagg
      121 ccatgccgag cgagacccag atcgaggcgg gaaaagccgg cacattgaag acgatcttcg
      181 gcacaatatc gctccaggcc cagtgaccgg aaagataaag gatcaggaaa agaaaataat
      241 agggaaaact aaagccaagc aggccaacga tccattcccg gacgcgaaaa ggccgcatgg
      301 tcagcagccc gaagagcacc agcagcagaa atgccaccgc cggaatatac agcagggaaa
      361 cgatgccggt ccaaagcccg atattgaaaa tggccgaacc gggccggggg ctgttataca
      421 gatccaacat ccggtaccac accaggacca tcaccgaatt gatcagcagg ggggctgaga
      481 aatggcccca gtccggaaga agggacgtga taagaatata agacatcccc ggcagatagg
      541 tcggcttcgg aaggaccttg tggtgattga aaatgctgtt gaacagggca gcctgggtaa
      601 agatcagcag gaaggtaagc agcgaaaaca ggatggggag ctgatcaaaa accgtattca
      661 gccccttcag gatgacccgg tagagataat tgtcttccgg atgcagggca ggcgccacgg
      721 gatgcaggaa caacggaaat ttcagcacca gggcataaca aagcaatacg acgatattgg
      781 caggattctt ttgtctgaag ataccgatca cggtaagggg gttggcatta gaaatctact
      841 ttcgcaaaac aaagataatt cctatacatg cacggaatga tagattccca actgctgacc
      901 tgtttgccat aacgcggcat gaattcatat ccccggtcaa gattatgcag ggtcggatag
      961 cgggttatcc tgccttccgg cgaaatactc tggtcgttga gcagcatatt cctgcgttca
     1021 tacaggttaa agaggaaatt cagctccctg cccgtattga ccatttcgtt ggagatcatg
     1081 ttatccgact cgtcgtcgaa ctggcttttg ggaatgatat tgctccactg caggttgctt
     1141 tcccggtcat aggaaaggac cataatattt tcggcatgaa atcgcgtccc gtacccgccg
     1201 tagttccttg cccaggggtt atagtaaggg ttgtagtacg gcgaataaaa ccccgggccg
     1261 tagccataga tagggttcat ataatcccac ctgttgaaca tgcttccccg ggaggtagta
     1321 tacagcgatt cggcgacaag caggtagccc ccatcccgtt tcgtgacgat ttgtttgata
     1381 aagaaatcat tgaaggccgt cttgatggtc gcgtcgctgc ttttggccag ggcgcggagc
     1441 tcatccgaga acaccgtcac cttttccgcc tgtttcgaat tggtcgcctt atcccaaaac
     1501 acggtataca gcccttcgat attgccgcgc cgctgcttat aatagaatcc tgaaataata
     1561 taccggttat tattgttatc gaccttgatc ttgatctcgt ccaggatgcg atcgccggcg
     1621 ccgatgtcat gtacggagaa agtgtcggcg gtgggtccct tcgtcacgac ggaaacgcgg
     1681 gaaacatagt cgttgctccc gctgcggagg tatttggcaa agacaaaagt tccttcgtta
     1741 tcgaccagga agtcggtaaa gagctcgtta cggtcttcca tcggcaggaa gagccggtgc
     1801 cggtcgacca gcttcagctc gctgtcataa aggaaagtgg tgaataagaa attcctcggg
     1861 tcatgactgt tgatcttgta gagcatgatc cgctgtttgt cctcactgac gatgaccgta
     1921 tagatcttgt tgttggacgc aaatccgacc tgcgtggtat ccagttccat gggcttgccg
     1981 atcagctggg cctggccgcc aagctttacc gcagcgatgt gcacgatgct ttttttctgg
     2041 tactcgtaaa tgagcaggaa ataatcctca tacgcgacga a
//
LOCUS       DATGKE010000037         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_922357, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000037 DATGKE010000000
VERSION     DATGKE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922357"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2195
                     /locus_tag="VKU83_00615"
     CDS             <1..2195
                     /locus_tag="VKU83_00615"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HLI92072.1"
                     /translation="KRYRNRNNPAVALIRQVIAHKEQNEPASYPFLSYDQYDKIRVLA
                     DRPPHWITDGKLFRRYHFLFEPDTTLVPGKRLVPAYLEETYSRQYFRKNPDRTKQVIL
                     AHKRVNLGEYVDMGGVSQIMNRLYEDFSVYDNTMLVFTLQFMSPVAKLGPDLYEYFIR
                     DTVVDKGVRTVRLDFAPRNAQDLLFKGTLYITLDGDYAIRSAELEASNHSNVNWVRSF
                     KVRQSFEKGPEGRYYRQSSDLLSYFSLTRNNAGFYGEHVVIRSNVSNTTLPAQVFRGL
                     PVDTTLASVRLPESSWEQLRPEPLNPSEERTYRNTDSLLQMRSYRRLMDWLVFATVGY
                     KAAGPFEIGPVGNFYSFNSLEGSRYQFGGRSTVKLSTRWFTDDYVAYATGDQRWKYYL
                     STTYSINHKSIYTYPFHYIQASYLHDTRNPGVENLFAQDNSFLGSFSHGIGGKWMYTD
                     IATISYIREFGDHFSYDFGGKYWLQEPAQNLFYIYDRPGSEADTVRRLTTTQLSVTLG
                     WSPHQRFYQGLTSRRVINTQYPAFTLQYALGIDGLLGGQDRYHAFHLGVAKRWYLAPL
                     GFMDIRFNAGYITGNLPFPLLIIQPANPSFFYSFNAYNLMNVEEFISDHYAALNVDHY
                     FNGFFLNKIPLLKKLRLREVVEGKVLVGGVRPENNPATNPAQMKFPLNNEGVLTTYPL
                     GNEPYIEAGVGIYNILNIFRLDLIKRFTYLRHPGISSTGIRLSTGLDF"
     gene            2329..2772
                     /locus_tag="VKU83_00620"
     CDS             2329..2772
                     /locus_tag="VKU83_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLI92073.1"
                     /translation="MTLKAMIGSFLLALVAATGWHYNLDEARKLAEQQHKHILLNFSG
                     SDWCGPCILLRKEILDNPGFLAMADTSLVLVNADFPRSKKNQPTKQQQAVNDAMADKY
                     DPQGKFPYTLLLNASGKVIRTWEGLPDKKPADFAMEVRSAIDSDQ"
     gene            2787..>3060
                     /locus_tag="VKU83_00625"
     CDS             2787..>3060
                     /locus_tag="VKU83_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLI92074.1"
                     /translation="MPQDPAAAVAFRKAFRKTLPLMGNRFELTVIAEQEAVAMDWIDA
                     GIAEIRRIERLLTTFSEDSETSLINRNAGITPVEVSSETFGLIERSI"
BASE COUNT          724 a          863 c          795 g          678 t
ORIGIN      
        1 agaagcgcta ccggaaccgg aacaatcctg ccgtagcgct gatccggcaa gtgatcgcac
       61 acaaggaaca aaatgaaccg gcctcgtatc cgttcctcag ctatgaccaa tacgacaaaa
      121 taagggtatt ggccgacagg ccgccgcatt ggataacaga tggaaagctg ttcaggcgct
      181 accatttcct gtttgaaccg gataccacgt tggtacccgg taagcggctg gttcccgcct
      241 atttggagga aacgtattcc cgacagtatt ttcgcaagaa tcctgaccgc accaagcagg
      301 tgatccttgc acacaaacgg gtcaacctcg gcgaatatgt cgatatgggc ggcgtcagcc
      361 agatcatgaa ccggctgtat gaagactttt cggtgtatga caatacgatg cttgtcttta
      421 ccctgcagtt catgagcccg gtagccaaac tcggtccgga cctgtacgaa tacttcatcc
      481 gggataccgt cgtcgacaag ggggtgcgca ccgtgcgcct ggactttgcc ccacgcaacg
      541 cgcaggacct gttatttaaa gggacgcttt atatcacgct cgacggagat tatgcgatcc
      601 ggagcgcgga gctggaggcg agcaatcaca gcaacgtaaa ttgggtaagg agtttcaagg
      661 tacggcagtc tttcgagaag gggcctgaag gcaggtatta ccggcagtca tccgacctcc
      721 tttcctattt cagcctaacc cggaataatg ccggcttcta cggcgaacat gtcgtgatcc
      781 gctcaaatgt ttcgaacaca acgctccccg ctcaggtttt tcgcggtctg cccgtcgata
      841 cgacgttggc atctgtccgg ctgccggaaa gctcctggga acagttgcgg cccgagccgc
      901 tcaatccttc ggaagagcgg acctacagaa ataccgacag cctgctgcaa atgaggtcct
      961 atcgccggct gatggattgg ctcgtttttg ccacggtcgg ttataaggcc gccgggcctt
     1021 ttgaaatcgg acccgtgggc aatttttatt cgtttaatag cctggaagga agccggtatc
     1081 aatttggcgg ccggtcgacc gtaaagctca gtacccgctg gttcacggac gactatgtgg
     1141 cctacgccac cggcgaccag cgatggaagt attacctgag cactacctac tccatcaatc
     1201 acaaatcgat ctatacctat ccgttccact atatccaggc cagctatctg cacgacacgc
     1261 gcaacccggg cgtggaaaat ctttttgccc aggacaacag cttcctgggt tcgttcagcc
     1321 atggaatagg aggaaaatgg atgtacaccg atatcgccac tatttcttac atccgcgagt
     1381 ttggcgacca tttttcctac gacttcggtg gaaagtactg gttgcaggaa ccggcgcaga
     1441 atctttttta tatttatgac cgccccggaa gcgaggcgga caccgtccgc cggctgacca
     1501 caactcaatt gtcggtaacg cttgggtggt cgccgcacca gcggttctac cagggcctga
     1561 cgtccaggcg ggtcatcaat acgcagtatc ctgccttcac ccttcaatat gcgctgggca
     1621 tcgacgggct cctcggcgga caggacaggt atcatgcctt tcacctcggc gtcgcgaagc
     1681 gctggtacct cgccccgctg ggattcatgg atatccggtt caatgcgggc tatatcaccg
     1741 gcaacctgcc ttttccctta ctgatcatcc aacctgcgaa cccttcattc ttttatagtt
     1801 ttaatgccta caacctcatg aatgtcgagg agttcatttc ggatcactat gcggccctga
     1861 atgtggatca ctatttcaat ggcttctttc ttaacaagat cccgctcctg aagaaactgc
     1921 ggctgcgcga ggtagtggaa ggaaaggttt tggtcggcgg cgtgcgccct gaaaacaacc
     1981 cggcaaccaa tccggctcaa atgaaattcc cactgaacaa tgaaggtgtc ctgaccacgt
     2041 acccactggg aaacgagccc tatatcgaag ccggcgtcgg catctataat atcctgaata
     2101 tattccggct cgacctcatt aaacgtttta cttatctgcg gcatcccgga atttccagca
     2161 cgggcatcag gctgagcacc gggctggact tctaagccgg ccgtgcgatc acggcatggt
     2221 tgggcggcag aagggccttt aacaaaaata taacggagcc cggcgctttt ggttatcttt
     2281 taagatttcg acgcaaccat gttgcaacac tgatcgtacc ttcgtacaat gacgctaaaa
     2341 gcaatgatcg gttctttcct cctggccctg gtggccgcga ccggctggca ttacaacctc
     2401 gatgaggccc gcaagctggc ggagcaacag cacaagcata ttcttttgaa tttttccggg
     2461 agcgactggt gcggcccctg catcctgctt cgaaaggaga tcctcgataa tcccggcttt
     2521 ctggcgatgg ccgataccag tctcgtcctg gtcaatgccg attttccccg gagcaaaaag
     2581 aatcagccga caaagcaaca gcaggcagtg aatgatgcca tggccgacaa gtatgacccc
     2641 caggggaagt ttccctatac cctgctcctg aatgcatccg gaaaagtgat ccgcacatgg
     2701 gaggggctgc ccgacaagaa accggcagat ttcgcaatgg aagtccgttc tgcaatcgac
     2761 agcgaccaat gaccgaacat acaattatgc cccaggatcc tgccgcagcc gtagcctttc
     2821 gcaaggcctt ccggaagacc ttgcccctca tgggtaaccg ctttgagctg acggtcattg
     2881 cggaacagga agcggtggct atggattgga tagatgccgg catcgccgag atccgccgga
     2941 tcgaaaggct cctcactacg ttcagcgagg acagcgaaac cagcctgatc aaccgcaatg
     3001 caggcataac gcccgtggaa gtaagcagtg aaacattcgg actgatcgag cgctcgatcc
//
LOCUS       DATGKE010000038         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1540835, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000038 DATGKE010000000
VERSION     DATGKE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540835"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VKU83_00630"
     CDS             complement(<1..344)
                     /locus_tag="VKU83_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HLI92075.1"
                     /translation="MASAEAEKLFQEKIGEDIRIEPKDWMPDAYRKTLIRQISQHAHS
                     EIVGMLPEANWITRAPTLRRKATLIAKVQDEAGHGLYLYSAAETLGISREEMIDQLHS
                     GKAKYSSIFNYPT"
     gene            540..1787
                     /locus_tag="VKU83_00635"
     CDS             540..1787
                     /locus_tag="VKU83_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HLI92076.1"
                     /translation="MKKLFLLFLFPILTQAGVQAQTEPQSDTGVHFEHGLSWADVLAK
                     AKAENRYIFMDCFTTWCGPCRYMSTTIFPQKQTGDYMNDKFVSVAVQLDTTAKDAPAV
                     KAWYADGHDLATKYGIRAYPTYMIFAPDGRPVHRLVGSRLDAKTFITDLATAFDSTQQ
                     YYTLLDQYNKGRRDTAFLHKMAMGCLNAYDVANGEKVADDWLGTQKDPFTRDALYLED
                     MYTRRSTDKYFAVFADHPAEVDKVLGAGVASQKVRSVYMMEGLGRNPGDNRPPDWTAL
                     RRKIAARVPAAADELTERIKIDYYQRRADWPNFQKAIVTYMKSYSRHMSDAELNQIAW
                     GVFQKCPDMSCVSDVLNWSKRLKDDKEPAFMDTYANILYKLGKREEAIALETRAADLA
                     ANGDKAGYQATIEKMKKGEKTWD"
     gene            complement(1837..>2249)
                     /locus_tag="VKU83_00640"
     CDS             complement(1837..>2249)
                     /locus_tag="VKU83_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLI92077.1"
                     /translation="KDWLEARGVERRKIHFELFHTLENAPAGEPEAVREAKATAQAGL
                     SRVTIRLDGLSHAFDLAYDGTSVLEAALWQGIDLPFACKGGVCCTCRAKLLEGKVEMD
                     HNYALEADELEAGYILTCQSHPRSAKLVIDFDAK"
BASE COUNT          504 a          661 c          614 g          470 t
ORIGIN      
        1 gtgggataat tgaagatcga ggaatacttg gctttcccgc tatgcagctg atcgatcatt
       61 tcctccctgc tgatccccag cgtttcagca gccgaataaa gatatagccc gtgcccggct
      121 tcatcctgca ccttcgcaat cagcgtagcc ttgcgcctga gcgttggagc gcgcgtgatc
      181 cagtttgctt ccggaagcat tccgacgatc tcgctatgcg catgctggga gatctgccgg
      241 ataagtgttt tccggtaggc atcgggcatc cagtctttcg gctcgatccg gatatcctcg
      301 ccgatctttt cctggaataa tttttcagcc tcggctgacg ccataggtca ttgatttgca
      361 cccaaagata ctttttttta cgaggctaac aattgttagt acttttagca agcggtgaaa
      421 gcacttgcgg aaatccggcg accccgactg acagcgcccc gcgtcccgcg gggcgttttg
      481 cgcgggagcg gtttttcggt cgaattccgc tacctttatc aaccaccaac attgatctca
      541 tgaaaaagct attcctcctg ttccttttcc ccatcctgac gcaagccggc gtacaggcgc
      601 aaacggagcc gcagtccgat accggcgttc acttcgagca cgggctcagc tgggcagacg
      661 tgctggccaa agcaaaggct gagaacaggt acatcttcat ggattgtttc acgacatggt
      721 gcgggccctg ccggtatatg tcgaccacca tcttccccca gaagcaaacg ggcgactaca
      781 tgaacgacaa attcgtcagc gttgcggtgc agctggacac gacggcaaaa gatgcgcccg
      841 ccgtcaaggc ctggtacgcc gacggacacg atctggcgac gaaatacggc attcgcgcct
      901 accccaccta tatgatcttt gcgccggacg gccgtccggt ccaccggctg gtcggatccc
      961 gcctggatgc gaaaaccttt atcacggatc ttgccacggc attcgacagc acgcagcagt
     1021 actatacctt gctggaccaa tacaacaagg gccgcaggga taccgccttc ctgcacaaga
     1081 tggctatggg ctgtcttaat gcctacgatg ttgcgaacgg ggaaaaagtc gccgatgact
     1141 ggctcggaac gcaaaaggac cctttcacgc gggatgcgct ctatctcgag gatatgtaca
     1201 cgagaaggag cacggataaa tattttgccg tatttgcgga ccatcctgcc gaagtcgaca
     1261 aggttctcgg tgcgggcgtt gccagtcaaa aggtccgcag cgtgtatatg atggaaggcc
     1321 tcggtcgcaa tccgggtgac aaccggcccc cggactggac agcattgcgc aggaagatag
     1381 ctgcccgcgt gccagccgcc gccgatgagc tgacggagcg cataaagatc gactactatc
     1441 aacgtcgcgc cgactggccg aatttccaga aggccatcgt gacatacatg aaatcatata
     1501 gccggcatat gtccgacgca gagctcaatc aaatcgcctg gggcgtcttc cagaaatgcc
     1561 ccgacatgag ctgcgtgtcg gacgtgctca actggagcaa gcgcctgaag gacgacaagg
     1621 aacccgcatt catggacacc tacgctaaca tcctgtataa attgggaaag cgggaggaag
     1681 cgattgcgct ggagacacgg gccgccgacc tggcggctaa cggcgataaa gcaggttatc
     1741 aggcaaccat cgaaaagatg aaaaagggag aaaagacctg ggattaaggg ctctccggcg
     1801 cctgaatcct atccggcccg gcgtcagccg ggccgtttat ttcgcgtcga aatcgatgac
     1861 cagctttgcc gaccgcgggt gtgactggca ggtcaggatg tatcccgcct ccagttcgtc
     1921 agcctccaac gcatagttgt ggtccatctc caccttacct tccagcaatt tcgcccggca
     1981 ggtgcaacac accccgccct tacaggcaaa gggcagatcg atgccttgcc acagcgcggc
     2041 ctcgagtacg gatgtaccgt catacgcgag atcgaaggca tggcttagcc cgtccagcct
     2101 tatggtcacc cggcttaagc cggcctgcgc ggttgctttg gcttcgcgga cggcctccgg
     2161 ctccccggca ggagcatttt ccagggtgtg aaacaactca aaatggatct tccttctttc
     2221 gacgcctcgg gcctccagcc agtccttaa
//
LOCUS       DATGKE010000039         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_502312, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000039 DATGKE010000000
VERSION     DATGKE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502312"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VKU83_00645"
     CDS             complement(<1..699)
                     /locus_tag="VKU83_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92078.1"
                     /translation="MRRSSLLAWLLAFFLGMVLFKPGLADAQPSTPDSTVRKTQPLIE
                     VLTGLNKSRGIFFLFSRGSLAGTPVDPPILSPGASIEKILTQVLRKSGLRYKKVDERT
                     FVILEKRPTAADSTFAPAVSELSPAPAGETRRGFVSGHVLASDGRNLQGVSITLGHTH
                     TGTTTDLAGYFEIGASGEDTLLFSFVGYRTKKLAADKLPPEGIILDPSDQPLTEVLIT
                     AMGIRKQERSLGFAT"
     gene            complement(720..1736)
                     /locus_tag="VKU83_00650"
     CDS             complement(720..1736)
                     /locus_tag="VKU83_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLI92079.1"
                     /translation="MIANYTTPEELLTDESFLSWYFRTVKDSGDDWDNWMKASPERRR
                     LVNEAVALLEAVRLPEVEPGGVDSNRLQAAEEALFSKLTEVSAGPGYKPQRLYGARRW
                     MVAAVLLLLLAGAVVVTVRIAGHSELRTSYGQIAQDQLPDGTHVTLDANTRLTWPAGW
                     KEGHDREVWISGEAFFHVRKTPTNSRFIVHTDHFDIIVTGTQFNVVNRHGNEHILLQE
                     GSVLLRGKDGSEMALKPGDFVRYDEKTMERKTVKADSVTAWKDQRLIFVRTPLRDLVT
                     IVNDHYGVDIQLGDASLGDSTITAILPNNNLDVLLQALEATTEFDIIQDGSRIRIVPH
                     HDHN"
     gene            1856..2452
                     /locus_tag="VKU83_00655"
     CDS             1856..2452
                     /locus_tag="VKU83_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI92080.1"
                     /translation="MNSAGQDITLWESFRKGDKEAFSSLFRQHYESLFRYGSKFTTNT
                     SVLEDTIQELFIDLWQSRSSTPVTSVRAYLLKSLKYKLLKAYRQTRSMVPMAESDGPF
                     EWSHESFLIAGEEDADKKRRLLKALSALTARQKEIVYLKYYQNLSYDEVSEIMNINYQ
                     AARNLLYQAIRSLKTLLPGPLQVIFLFSVMSTLHFALL"
     gene            2496..>2977
                     /locus_tag="VKU83_00660"
     CDS             2496..>2977
                     /locus_tag="VKU83_00660"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HLI92081.1"
                     /translation="MSMVDSFEKIPVKIFGSLEEGSRFIGKEIATLIREKEKKGEKTI
                     LGLATGSSPKSLYAELVRLHREEKVSFRNVITFNLDEYYPIDNDALQSYNRFMKEQLF
                     DKIDIDPANCHIPNGQWKKEEIKQRCIEYEARIEKAGGIDLQILGIGNNGHIGFNEPG
                     S"
BASE COUNT          716 a          815 c          762 g          684 t
ORIGIN      
        1 ggtggcgaaa cccagggacc gctcctgttt gcggataccc atggccgtga tcagcacttc
       61 ggtgagcggc tggtcggatg gatcgagaat gatgccttcc ggcggcagtt tgtccgccgc
      121 cagctttttg gtccggtaac cgacgaagga aaagagcagc gtatcttctc ccgaggcgcc
      181 gatctcgaaa tagccggcaa gatcggtagt cgttccggta tgcgtgtgcc caagggtaat
      241 gctcaccccc tgcaggttcc tgccgtcgct ggccagtacg tgaccggaaa caaagccgcg
      301 acgggtctct cctgccggcg ccggcgaaag ttccgatacg gcgggggcga atgtactgtc
      361 ggctgcagta gggcgttttt ccaatatgac gaacgtacgc tcgtcgacct tcttatagcg
      421 aagcccggat tttcggagaa cctgtgtaag tatcttttcg atgctggcgc cgggagaaag
      481 gatcggcgga tccaccggcg taccggcgag cgacccgcgg gaaaaaagaa aaaagatgcc
      541 cctgcttttg ttgagcccgg taaggacttc gatcaacggc tgtgttttcc ggacagtgga
      601 atcgggtgtg gagggctgtg cgtcagccag ccccggcttg aacaggacca tgccgagaaa
      661 aaatgcaagc agccaagcga gtaagcttga tcttctcatg acgcagcggt tgaaagtctt
      721 tagttatggt cgtggtgcgg cactatcctg atcctgctac cgtcttggat gatgtcgaat
      781 tcggtagtag cctccagtgc ctgtaatagc acatccaggt tattattcgg taatatagcc
      841 gttatcgtac tatcgcccag cgaagcatct ccgagctgta tatcgactcc ataatgatcg
      901 ttgacgatcg ttaccagatc gcgcaggggc gtccgcacga aaataagccg ctgatctttc
      961 caggcggtca cgctgtcggc cttaacggtc ttccgctcca tagtcttttc atcgtatcgt
     1021 acaaaatcac ccggcttcag cgccatttca ctgccgtcct ttccgcgcag cagaacgctg
     1081 ccttcctgta aaaggatatg ctcattgcca tgccggttga cgacattgaa ctgcgtgccg
     1141 gtgacgatga tgtcgaaatg atcggtatgt acgatgaaac ggctgtttgt cggcgttttc
     1201 cggacatgaa aaaatgcttc cccgctgatc cagacctcgc ggtcatgccc ttccttccat
     1261 ccggcgggcc aggtcagccg ggtattggca tcgagcgtaa catgggtacc atcgggcagc
     1321 tggtcctggg cgatctgtcc gtaagaggtt ctcagttcgc tatgccctgc tatacggacc
     1381 gtcacaacga cggcgccggc cagcagcagc aatagaacgg cagctaccat ccagcggcgg
     1441 gcgccatata ggcgttgcgg tttatagccg ggcccggcgg aaacttctgt taacttgctg
     1501 aataacgcct cttcggccgc ctgcaaccga ttgctgtcta ctccccccgg ctccacttcc
     1561 ggaagacgga ctgcttcgag caaagccact gcctcattca ccaaccttcg ccgctccgga
     1621 ctggccttca tccaattgtc ccaatcgtcc ccgctatcct tcaccgtacg gaaataccag
     1681 gaaagaaagg attcatctgt taaaagctct tccggtgttg tataattggc tatcatgcga
     1741 tcgatactga acgggaaatt aactcctaaa gatagtcctg aaacggcgga ctgccccgtt
     1801 ttttccattt tttttagcga tttatcctaa attacgcgtc taaccacgaa cctctttgaa
     1861 tagtgcaggc caggacataa cgctatggga atcctttcgg aagggggaca aagaagcctt
     1921 ttcgtccctt ttccggcagc attatgaaag cttgttccgc tacggcagca agttcacgac
     1981 caatacaagt gtattggagg acactatcca ggaactgttc atcgatcttt ggcagtccag
     2041 atccagcacc ccggtgacat ccgtgcgtgc ctacttgctc aaatccctga aatacaaatt
     2101 gctgaaagcc taccggcaaa cacggtccat ggtaccgatg gctgaaagcg acgggccgtt
     2161 cgaatggagc cacgagagct tcctgatcgc gggcgaagaa gatgccgaca aaaaaagacg
     2221 cctcctcaaa gcattatccg cactgaccgc ccggcagaaa gagatcgtct atcttaaata
     2281 ttatcagaac ctgagttacg atgaggtgag cgagatcatg aacatcaact atcaggccgc
     2341 acgaaacctg ctctatcagg ccatccgatc attgaagacc ctcttgcccg gcccgctgca
     2401 ggttattttt cttttttcag tgatgagtac attacatttt gccctccttt agcacctttc
     2461 cattaatttc gcgcatcata caaaacatct gatctatgag catggtagac tcattcgaga
     2521 aaatcccggt aaaaattttc ggttcactgg aagaaggctc ccgcttcatt ggcaaggaga
     2581 tcgcgacgct tatccgcgaa aaggaaaaaa aaggcgagaa gaccatttta ggtcttgcca
     2641 ccggctcctc tcccaaatcg ctgtacgcag agctggttcg gctgcaccgg gaagagaagg
     2701 tcagcttccg gaatgtgatc acctttaatc tggatgaata ctatcccatc gacaacgatg
     2761 ccttgcagag ctacaaccgc ttcatgaagg agcagctgtt cgataagatc gatatcgatc
     2821 ccgccaattg ccatatacca aacggccagt ggaaaaaaga agagatcaaa caacgctgta
     2881 tcgaatacga ggcacgcatc gaaaaagccg gcggcatcga cctccagatc ctggggatcg
     2941 gcaacaatgg tcatatcgga ttcaatgaac ccggctc
//
LOCUS       DATGKE010000040         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_608738, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000040 DATGKE010000000
VERSION     DATGKE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608738"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VKU83_00665"
     CDS             complement(<1..1127)
                     /locus_tag="VKU83_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HLI92082.1"
                     /translation="MKKLIFFIGLPLMAGITLVRGQAGLRPVYRDTMGDTMAAPRNFG
                     RAALTFGLGEAGPFLFDRYWLQRDYARISFQSIGYNLKPSSWAWDDDPFQTNEFGHPY
                     HGSIFFNALRSNGYGFWQSAAAAFAGSYLWESFAENQAPAPNDFINTGFGGMVLGEMT
                     HRMAGKLVKNRSRGIQRQANEFLALLINPSEGLTRIVTGRWGRPAPVSEYDATPMSID
                     VEGGIRKYSPDHANPFSDGRFAVYGRLHLLYGSPYQDLNTPFSNLHITIEAGQDDSSK
                     VNIVSVYGSLTGWKLHLAGRQQAAVVSANYDYINNEAFFYSAQSIRMNFYSDFALPAR
                     IRINTQLAAGPVILAAVPDGYLFEGRDYDYGPGLAIHASATV"
     gene            1372..2592
                     /locus_tag="VKU83_00670"
     CDS             1372..2592
                     /locus_tag="VKU83_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92083.1"
                     /translation="MRQLRSTVRGIALVLTIANNPFFATAQAVTVDSSVLDRLTALEQ
                     QVADHKPGESHFLVVGLTTFGFVRSKTTVTPPGGSPQAATIVSLGDADRYEFSPMLLW
                     RHGTKWLMEFEPSFDGNSLGVNWADIHYFACPGLQIRAGYFVLPFGIYNKRLAAGWID
                     KLAPDPQGIDLPGSDFGIGISGGLPLGNMKWSYDASLTNGLQLLPDGELQGVGIVDNN
                     NNKTVTGRLALLPFSNSSLEIGASGLYGGVADAGSPYSNANATMYGADLNFVKTVSPI
                     LINIKSQYSRLAVNSQQFVKPTDSTAFTFDNKQTSFFGQISLRPTGSGNKILSNLEAA
                     FRYVNYQTPAGSFWGSNYHEEDLGLDYWFSWRTVLKFTYAWSKGTDSSNPSGGTNGTV
                     TGMNNLYIQFSIQL"
     gene            2597..2872
                     /locus_tag="VKU83_00675"
     CDS             2597..2872
                     /locus_tag="VKU83_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLI92084.1"
                     /translation="MRVYHFRNTIIGIIGVSAVAVALLLTSCVESKQVAAKSGAQLWA
                     ENCQRCHNTPSPTTFSHEQWITVGMHMQTRAQLTQQERDKIVAFLSQ"
     gene            complement(2882..>3154)
                     /locus_tag="VKU83_00680"
     CDS             complement(2882..>3154)
                     /locus_tag="VKU83_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLI92085.1"
                     /translation="FRIKKGKRYRLHMRNASDDIHPMHLHRHSFELTRYAGKPTSGIF
                     KDVAMLGGFQEMDVDFVADNPGMTLFHCHMQLHMDYGFMALFDYTG"
BASE COUNT          739 a          885 c          837 g          693 t
ORIGIN      
        1 acagtcgccg acgcatggat ggccaggccc ggcccataat catagtcccg gccctcgaac
       61 aagtatccgt cgggtaccgc tgccaggatc accgggcccg cggccagctg cgtgttgatc
      121 ctgatcctcg ctggcagggc gaaatcggaa taaaagttca ttcggatgct ttgagcgcta
      181 taaaaaaagg cttcattgtt gatgtagtcg tagttagcgg acacgaccgc cgcctgttgg
      241 cggccagcaa ggtgcaactt ccatcctgtc aatgacccat aaacgctcac gatgttcacc
      301 ttcgagctat cgtcctgacc tgcctcaatg gtgatatgaa gattgctgaa cggggtgttg
      361 agatcctgat atggcgatcc atacaagaga tgtaaccgtc cgtacacggc gaatcgcccg
      421 tccgaaaatg gattggcatg gtccgggctg tacttgcgta tccccccctc cacatcaatc
      481 gacatgggcg tcgcatcata ttcgctcacg ggtgccgggc ggccccatct gcccgtcacg
      541 atgcgggtca aaccctccga cgggttgatg agcaacgcga ggaactcatt ggcctgcctt
      601 tggatcccgc ggctccggtt cttgacaagt ttacctgcca tgcggtgtgt catctcgccg
      661 aggaccattc ccccgaagcc tgtattgata aagtcattcg gcgccggcgc ctgattttcc
      721 gcgaagcttt cccaaaggta gcttcccgcg aatgcagctg cagccgattg ccagaaacca
      781 tagccgttgc tccgcaaagc attgaaaaaa atgctgccgt gatacggatg gccgaactcg
      841 ttggtctgaa aggggtcgtc gtcccatgcc cagctggaag gcttgaggtt gtacccaatc
      901 gattggaagg atattctcgc ataatcccgt tgaagccagt aacggtcgaa aagaaagggg
      961 ccggcctcgc ccaacccaaa tgtcaatgcg gcccggccaa aatttctggg tgctgccatc
     1021 gtatccccca tcgtatcccg gtaaacaggg cgcaacccag cctgcccccg gacaagagtg
     1081 atgcctgcca tcagcggaag cccaataaaa aagatcaatt tcttcatgtt tggtgcgacc
     1141 ggtggctcag cccgatcggt ccgatcccgc taaactatcc gttcggttgg tttaaatacc
     1201 tgacgccggt caatcccttc cttgactcaa ggcggagtat gaatgtttgt ctttcgattg
     1261 accattgtca tttaagcgcg tgacgagggt cattcggctc ctatatcggg ccatatactt
     1321 ttacgcaacc ggaaattcat ccgtcgataa ataactcaaa caacagacta tatgagacaa
     1381 ttgcgctcaa cggtccgggg gatcgcattg gtcctgacga tcgctaacaa tccatttttc
     1441 gcgaccgccc aggccgtcac cgtcgactct tcggttctcg accggctaac ggcgctggaa
     1501 caacaggtgg ccgatcacaa acccggcgaa agccatttcc tcgtggtggg cctcacgaca
     1561 ttcggatttg tccgcagcaa gaccactgta acaccgccgg gaggaagtcc gcaggccgcc
     1621 acgatcgtga gccttggcga cgcagaccgc tatgaattca gcccgatgct gttgtggcgg
     1681 catggtacga aatggctgat ggaatttgaa ccatccttcg acgggaacag ccttggcgta
     1741 aactgggccg atatccacta tttcgcctgt cccggattac agatcagggc aggatatttt
     1801 gtgcttccct tcggcatcta taacaaacgc ctcgccgccg gatggatcga caaacttgca
     1861 ccggaccccc agggcataga cctgcctgga agcgattttg ggatcgggat cagcggcggg
     1921 ctgccacttg gcaatatgaa atggtcctat gatgcaagcc tgaccaatgg gttgcagctg
     1981 ctccccgacg gggaattgca gggcgtgggc atcgtggaca acaataacaa taagacggtc
     2041 accggccggc tggccttgct tccgttcagc aattcctccc tggaaatcgg cgcatccggg
     2101 ctgtatggcg gcgtcgccga cgccgggtcg ccttattcaa atgcgaacgc caccatgtat
     2161 ggcgccgacc tcaatttcgt gaagactgtc agccccattt tgatcaatat caaaagccaa
     2221 tacagccgcc tggccgtgaa cagccagcaa tttgtcaagc cgactgattc gaccgccttc
     2281 acgtttgata acaaacaaac gtccttcttt gggcaaatct cgcttcggcc taccgggtcg
     2341 gggaacaaaa tcctgagcaa tctcgaagcg gcgttccggt atgtcaatta ccagacgccc
     2401 gcaggatcgt tttggggatc caactaccac gaggaagacc tgggcctgga ctactggttt
     2461 tcctggcgga ccgtgctgaa atttacttat gcctggtcaa agggcaccga tagcagcaat
     2521 ccctccggtg gcacgaacgg aacggttacg ggaatgaata acctttatat acagttctct
     2581 attcaactat aattatatgc gagtatatca ctttagaaat acgatcatcg gcattatcgg
     2641 cgtaagcgcg gtggccgttg ccctcctgct gaccagttgc gttgaaagca agcaagtcgc
     2701 ggccaagtcg ggcgcccagc tatgggcaga gaattgccag cgatgccata acaccccgtc
     2761 gccaactacg ttctcacacg agcagtggat caccgtaggg atgcacatgc aaactagagc
     2821 gcagctcacg cagcaggaaa gggataagat cgtcgcattt ctttctcaat aaggacaatg
     2881 attagccggt atagtcgaag agcgccatga aaccgtagtc catgtgaagt tgcatgtggc
     2941 agtgaaaaag ggtcatgccg ggattgtcgg cgacgaaatc cacgtccatt tcctgaaagc
     3001 cgcccagcat ggcaacatcc ttgaagatgc cggaagtagg tttgccggca taccgcgtca
     3061 actcgaagct atgccggtga aggtgcatcg gatggatatc gtcggaagca ttgcgcatgt
     3121 gcaggcggta ccttttccct tttttgatac ggaa
//
LOCUS       DATGKE010000041         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_409492, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000041 DATGKE010000000
VERSION     DATGKE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409492"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VKU83_00685"
     CDS             complement(<1..256)
                     /locus_tag="VKU83_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLI92086.1"
                     /translation="MRFTFYGHATFAIEISGKVFLFDPFVTGNPSAKDIAIQRIKADY
                     IFVTHGHGDHTADLVDVAKQTGAQCVAGFEIATWLQNMGVE"
     gene            358..906
                     /locus_tag="VKU83_00690"
     CDS             358..906
                     /locus_tag="VKU83_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLI92087.1"
                     /translation="MRKLTGTLFLSLLLAGLSFRASAQGISGDQIKTQFIADWERAKS
                     YTQDYLNTMPADKYSFKAVDSIRSFAQQMLHLASGILFHISAATGEKAPWSGRGLETR
                     PTAQNKDSVVWYVNSAYDFALESVKKLDPNKYGEMVGQAPRQVTRFAMLLKAFEHQTH
                     HRGQTTIYIRLQGIRPPNERLY"
     gene            complement(915..1985)
                     /gene="namA"
                     /locus_tag="VKU83_00695"
     CDS             complement(915..1985)
                     /gene="namA"
                     /locus_tag="VKU83_00695"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF010047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HLI92088.1"
                     /translation="MSKLFTPLEIRDIVLGNRIVVSPMCQYSSNDGMADDWHLVHLGT
                     RAVGGAGLVFTEATAVTPEGRISPHDLGIWKDAHIEGLRRIVKFVRAQGSEIGIQLAH
                     AGRKASVREPWIGGGLVPEKEGGWPVIAPSPIAFDEHYGKPVELTVAAIRQVVDDFRA
                     AARRAMLAGFKIVEIHAAHGYLIHQFLSPLSNLRRDEYGGGFDNRIRLLLEIVDAVRA
                     VWPPGYPIFVRISATDWADGGWSVEEAVQLAGVLKSHAVDVIDCSSGGLVPYQKIPVG
                     PGYQVNFARQIREATGILTAAVGLVTEALQAETILATGQADLIVMARQLLRDPYFPLH
                     AADELRYKDIHWPLQYERARLK"
     gene            2029..>2263
                     /locus_tag="VKU83_00700"
     CDS             2029..>2263
                     /locus_tag="VKU83_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLI92089.1"
                     /translation="MPKPFPMLLAVTTLLLAAKPPAASAQVLVAAAADLRFAMDSLVD
                     VFSRVNPGITVKPTYGSSGNFFEQIRNGAPFDIF"
BASE COUNT          504 a          716 c          535 g          508 t
ORIGIN      
        1 tctccacccc catgttttgg agccaggtgg ctatttcaaa acccgctaca cactgcgcac
       61 cagtctgctt ggccacatcc accaaatcgg cagtatggtc cccatgccca tgcgtcacaa
      121 agatataatc cgccttaatt ctctgaatag caatatcctt cgccgacgga ttaccagtaa
      181 cgaacggatc gaataaaaag accttcccac ttatctcgat cgcaaaggtg gcatggccat
      241 aaaaagtaaa tctcatgtgc aacttcttaa aagtatgtta aaacaaaagt actccggaat
      301 agttcgcaac cgcttatctt taaggtccgt tttttaccac caaataacca aatctatatg
      361 cgcaaactta cagggaccct cttcctttct ctcctgcttg caggtctttc cttccgggct
      421 tccgctcaag gcatttcagg cgatcagatc aaaacgcagt tcattgccga ctgggaacgg
      481 gcgaaatcct atacgcagga ttatctgaac actatgcccg ctgacaaata ttcctttaag
      541 gccgtagata gcatccgtag cttcgcgcaa cagatgctcc atctcgccag cgggatctta
      601 tttcatattt ccgccgctac cggcgagaag gccccatggt ccggtcgcgg actcgagacc
      661 aggccgaccg cacagaacaa ggactcagtg gtctggtacg tgaatagcgc ttacgatttc
      721 gccctcgaaa gcgtcaaaaa gctcgacccc aataaatatg gcgaaatggt cggccaggcg
      781 ccccggcaag tcacccgttt tgccatgctg ctgaaagcct ttgaacatca aacgcatcat
      841 cgcggtcaga ctaccatcta catccgcctg caaggtatcc gtccgcccaa tgagcgcctg
      901 tattgatcgg tgacctattt caaccgggcc cgctcgtatt gcagcggcca gtggatatcc
      961 ttgtaacgca gctcatcggc cgcatgcagc ggaaagtaag gatcacgcag caattgccgg
     1021 gccatcacga tcagatcggc ttgccccgtt gctaaaatgg tttcggcctg cagcgcctcg
     1081 gtgaccagcc cgaccgccgc cgttaggata cccgtcgctt ctctgatctg ccgcgcaaaa
     1141 ttcacctggt agccgggacc tacgggtatt ttttgatacg gaaccagccc gccgctggaa
     1201 caatcgatca catccacggc gtgggacttc aatactccgg ccagctgtac tgcctcctcc
     1261 accgaccagc cgccatcggc ccagtccgtg gcggagatcc gtacgaagat aggatacccc
     1321 ggaggccaga ccgccctcac cgcatcgacg atctccagca gcaatcgtat ccggttgtcg
     1381 aaaccgccgc catattcgtc ccgtcgcagg ttgctgagtg gagaaaggaa ttgatggatc
     1441 aggtatccat gagccgcatg gatctcgacg atcttgaaac ccgccagcat ggcccgccgg
     1501 gcagccgctc tgaaatcgtc gacaacctga cggatagccg ctacggtgag ttccaccggc
     1561 ttcccataat gctcatcgaa tgcgatcggg ctgggggcga tcaccggcca gccgccttct
     1621 ttctcgggca ccagcccacc accgatccag ggttccctga cactggcctt ccggcctgca
     1681 tgcgccagct gtatgccgat ctcgctcccc tgcgcccgta cgaatttcac gatccggcgc
     1741 agcccttcaa tgtgcgcgtc tttccagatg cccaggtcat gcggcgagat ccttccttcc
     1801 ggcgtcactg ccgtcgcttc tgtaaagacc agacccgctc cgcctacagc cctggtgccc
     1861 agatggacca ggtgccagtc atcggccatt ccgtcgttcg aagaatattg gcacatcggc
     1921 gatacgacga tccggttgcc taaaacgata tctcttatct ctaaaggagt gaacagtttg
     1981 ctcatattac cggcgaaggt cctaaattta tgtataattc tatcttccat gcccaagcct
     2041 tttccaatgc tgctcgccgt gacgacgctc ctgctggccg cgaaaccacc ggctgcatcc
     2101 gctcaggtgc tcgttgccgc ggccgccgat ctgcgattcg ccatggattc tctcgtcgac
     2161 gtcttttcgc gggtgaatcc cggtatcacg gtcaagccta cctatggatc atccggaaat
     2221 ttcttcgagc agatccgcaa tggcgccccc ttcgatattt tct
//
LOCUS       DATGKE010000042         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_712682, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000042 DATGKE010000000
VERSION     DATGKE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712682"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VKU83_00705"
     CDS             <1..277
                     /locus_tag="VKU83_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92090.1"
                     /translation="LLTAGMEVSNSLYSYQTALEKQGTRREEIQSLEKAVDFTKELLK
                     FSSATNYTDVLTSEQSLLAAQLDGVNDRLQELQAIVDLYRALGGGWQ"
     gene            complement(293..>2143)
                     /locus_tag="VKU83_00710"
     CDS             complement(293..>2143)
                     /locus_tag="VKU83_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92091.1"
                     /translation="LPFRIGGVYQDLPRNSTFHNLQAIMPWDCNANSYRRDNTDWQDH
                     NSQLYVELAPGVTAEQATARVKDLPGTYNKVLHEETLVYPIDKAYLYDYDHFSMNKPD
                     GGRIRIVWLFGIIGVFVLFLACINFMNLSTARSEKRAKEVGIRKTVGSLRTQLIAQFL
                     SESSLIAFLALFGALLLVYISLPFFNGLAGKEMAIPWSSPVFWLAIFGFTLFTGLLAG
                     SYPAFYLSSFTPVYVLKGTLGAGRPRSLPRQVLVVIQFTVSLTLIIGTSTVYRQVLFT
                     KDRPVGYHREGLFTVAMHTPDSYRNYEALRTELIQKGLAADVAASDMAPTSFKDNNSL
                     DWSGKRPEQNLIGFWNVNITRDYGRTIGWTITRGRDMSRDFATDSNAAVLNEKAARAI
                     GFKDPIGQVVKLSGKPYTIVGVSADMVNNSPYDTVEPAVFVAGGYTDEMIVKIKPGIN
                     PHTAIARMEPIFKKYNPSSPFMYKFVDETYETKFEAEERIGNLSAVFAGLAIFISCLG
                     LFGLAAFIAEQRTKEIGVRKVLGASIVGIWALLSKEFLKLTVISIIISVPPAYYFMHK
                     WLAGYHYQAPLSWWIFASPGAGIVAIALLTVSYQSLRAALMNPVKSLRTD"
BASE COUNT          514 a          529 c          622 g          478 t
ORIGIN      
        1 tttgctgacg gccgggatgg aggtgtcgaa cagcctgtac agctatcaga cggcccttga
       61 gaagcaaggc acccgccggg aggagatcca gtcgctggag aaggcggtgg attttacgaa
      121 agagctgctc aaattcagtt cagcaacgaa ttatacggat gtcctgactt ccgagcaatc
      181 gctgctggcc gctcagctgg atggcgtaaa tgaccgcttg caggaattac aggcgatcgt
      241 cgatctgtat cgggcgttgg gcggggggtg gcaatagcgg atgtttgaca tcctagtccg
      301 ttcgcaggct tttgaccggg ttcatgaggg cggctctcaa tgactgatag ctcacggtta
      361 ggagtgcaat ggcgacgatg ccggctccgg gagaggcaaa gatccaccac gagagcggag
      421 cctgataatg ataaccggcc agccatttat gcatgaaata ataggccggc ggcacggata
      481 taatgatcga tatcacggtg agtttgagaa attccttact aagcagagcc catattccca
      541 cgatgcttgc cccgagcact ttccggacgc cgatttcttt tgttcgttgt tccgcgataa
      601 atgcagccag accgaacagg cccagacagg atatgaagat tgccagtccg gcaaatacgg
      661 ctgatagatt gccaatgcgc tcctcggctt cgaattttgt ctcgtaggtc tcgtcgacaa
      721 atttgtacat gaagggactg gaaggattat attttttgaa aatgggttcc atccgcgcta
      781 tggcggtatg cgggttgatc ccgggcttga tcttgacgat catctcgtcc gtatagcctc
      841 cagctacaaa gacagcaggt tcaaccgtat cgtacgggga gttattaacc atgtccgctg
      901 agacgccgac gatcgtgtaa ggcttaccag acagtttcac gacctgacca atggggtcct
      961 tgaagccgat cgccctggcg gccttttcat ttaagacggc agcattgctg tcggtggcga
     1021 aatcgcgcga catatcgcgt cctctcgtga tggtccagcc aatcgtgcgg ccgtagtcgc
     1081 gagtgatatt gacattccaa aatccaatga ggttttgctc cggccgtttt ccagaccagt
     1141 ccaggctatt attgtcctta aaggaagtag gggccatatc ggaggcagcg acgtcggccg
     1201 ctaggccttt ctggatgagc tccgttcgaa gcgcctcata gttcctgtag ctgtccggcg
     1261 tgtgcatcgc gaccgtgaac aagccttccc ggtggtagcc gacgggacgg tccttggtaa
     1321 ataagacctg tctgtaaacg gtgctggtcc caataatcag tgtgagtgat acggtgaatt
     1381 ggatgacaac aagcacctgc cgcggaaggg aacgaggtct cccggccccc agcgtgcctt
     1441 tcaatacata cacgggggtg aaggaagaaa gatagaatgc aggatatgag cccgctaaca
     1501 ggccggtgaa caaagtgaag ccgaagatgg ccagccaaaa gaccggactg ctccaaggaa
     1561 tggccatttc cttgcctgca agcccattga agaatggaag gctaatatag accaggagca
     1621 gggcgccgaa aagggcgagg aaggcaatga gagaagattc gcttaggaat tgtgcgatca
     1681 gctgggtccg aagcgaaccg accgttttgc ggatgccgac ctctttcgcc cgcttctcac
     1741 tgcgcgccgt gctcaggttc atgaaattga tacaggccag aaacagcacg aagacaccga
     1801 tgatgccaaa aagccaaaca atgcgaatgc ggccgccgtc tggtttgttc attgaaaaat
     1861 gatcatagtc atacagatac gccttgtcga tgggatacac gagggtctct tcgtgaagga
     1921 ctttattgta agttccaggc aaatctttta cccgtgcagt tgcctgttcg gcggtaacgc
     1981 cgggcgccaa ctcgacatac agctgactgt tatggtcctg ccagtcggtg ttgtcccggc
     2041 ggtaactgtt ggcgttgcaa tcccagggca tgatcgcctg caggttgtgg aaggtgctgt
     2101 tgcggggaag atcctgatac acaccgccaa tgcgaaaagg gag
//
LOCUS       DATGKE010000043         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1539181, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000043 DATGKE010000000
VERSION     DATGKE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539181"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1430)
                     /gene="nusA"
                     /locus_tag="VKU83_00715"
     CDS             complement(183..1430)
                     /gene="nusA"
                     /locus_tag="VKU83_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLI92092.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDE
                     NFDVIVNAEKGDLEIMRRRTIVNDGEVNDPLAEIAYTDAVKIEPDYEVGEELYEEVEI
                     LDFGRRAILAAKQTLASRIGDLKKNVLVKKYSERVGEIVSAEVYQVWKKEILLLDEDG
                     SELILPKSEQIPQDYFKKGESIRAVVKKVELKNNSPVIILSRTSPLFLSKLLEIEVPE
                     IFDGLIVIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTANTQLLIQRSLTPSKITNMELDNEKQHANVYLKPDQVSLAIGRKGVNIKLAQEL
                     TGYTIDVFRDNEGEPEEFDIDLEEFADEIETWVIDELKRIGCDTARSVLDLTADELER
                     RTDLERETIDDVLRILKEEFDKG"
     gene            complement(1448..1915)
                     /locus_tag="VKU83_00720"
     CDS             complement(1448..1915)
                     /locus_tag="VKU83_00720"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92093.1"
                     /translation="MDSEAIKTVEGLVGEVLTAEPGYFLVEVRIKPTRNVKVFIDGDE
                     GISIEKCVRFNRALYKKIEASGIFPPGDFSLEVSSPGLDEPLKLHRQYKKNVGRLVEL
                     VLQDGSKYGGRLLEVSEDGIIIEESRGRNKKKETVNHAFLFDNIKSTRIQVVF"
     gene            2040..2396
                     /locus_tag="VKU83_00725"
     CDS             2040..2396
                     /locus_tag="VKU83_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92094.1"
                     /translation="MGEPQNLGSFIAGNKDLVKEYLETRLEIYRLQTVRVVAKSAGLV
                     AWIFLSLLLGFLFLFFCGLMLGCWFAGIFHSYVTGFGLVALLILAVFILVAIFRRSLF
                     VNPVIQAIIQKSKDGE"
     gene            2409..2726
                     /locus_tag="VKU83_00730"
     CDS             2409..2726
                     /locus_tag="VKU83_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92095.1"
                     /translation="MSKAARSLQELDAEIAALKQKARRLEDELDESWAYFQEHSGQMF
                     VRSILPRSLEGLVPLTGIRVLDAILQSERLQRIIERLAGRSAAWLGDGLNWLTNRIFK
                     DEG"
     gene            2791..3078
                     /locus_tag="VKU83_00735"
     CDS             2791..3078
                     /locus_tag="VKU83_00735"
                     /inference="COORDINATES: protein motif:HMM:NF027444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HLI92096.1"
                     /translation="MMLADLLFLVLVGYLLYRFIYGFVVPLMRASRQVREQFRNMQRP
                     SNDSSGYEESQRHSGTQRPHSGAQQPRSGGSGPKRGFKAPAEDYIDFEEIK"
     gene            complement(3092..3709)
                     /locus_tag="VKU83_00740"
     CDS             complement(3092..3709)
                     /locus_tag="VKU83_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLI92097.1"
                     /translation="MIGIKNIIFDLGGVIINLDNRRTEDAFTALGVKDFRKYFGHGFA
                     ESFFRDYEVGKLTDQEFIHSIRRFGALDQVSDQAIVDSWNALLLDFPPERIRLLTELR
                     KTYRLFLFSNTNALHLAALRQIWTRSFGSGSLEDHFEKAYYSHIMGMRKPDPESFQYI
                     LQENNLKGNETLFVDDAIVNVEGAEQAGLRGLFLRPGIGLMDFQW"
     gene            complement(3783..>4467)
                     /locus_tag="VKU83_00745"
     CDS             complement(3783..>4467)
                     /locus_tag="VKU83_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLI92098.1"
                     /translation="KTGKPVVLVLFTGRPLAIKWENDAVPAILNVWFGGTEAGHAIAD
                     VLFGDVNPSGKLSTTWPQNVGQIPLYYNHMNTGRPLEGQWFQKFRSNYLDVSNDPVYP
                     FGYGLSYAHFTYSGLTLSSSSLKGNQRLTATVTVTNDGGVDGREVVQLYIRQLVGAMT
                     RPVKELKGFEKIALKAGESRKVSFTVTTGMLKYYTNDLKYDWEGGDFLIMVGGNSRDV
                     LTEKVNWTK"
BASE COUNT          968 a         1213 c         1145 g         1141 t
ORIGIN      
        1 ataatacaaa tgcgagaaat cgctttaaag aacattagcg aatctctcgc taaccgctaa
       61 tgtttgcaca ggccggagaa ccttgactca aaaacccgtt aaaaacaggt tcgcaacctt
      121 tcggtagtgg ccggcgccac gaaaataaaa ctatttggcc gttatctatt tccccggcca
      181 tattaacctt tgtcgaattc ttctttcagg atgcggagca catcgtcgat cgtctcccgc
      241 tccaggtctg tccttctttc gagctcgtcc gctgtcagat caagaacgct ccgtgcggtg
      301 tcacacccga tccgcttcag ctcatcgatg acccaggtct cgatctcgtc ggcaaattct
      361 tccaggtcga tatcgaactc ttcgggctcg ccctcgttgt cgcggaatac gtcgatcgtg
      421 taccccgtca attcctgcgc cagtttgata ttcacgccct tacgcccgat tgccagggaa
      481 acctggtcgg gtttcaaata cacattagcg tgttgctttt cgttgtccag ctccatattg
      541 gtgatcttgg aaggcgtcag cgaccgctga ataagcagct gggtattggc ggtccagtta
      601 atgacgtcga tattttcatt cttcagctcc cgaacgatgc catgaatacg gctgcccttc
      661 atccctacgc aggcgcccac cggatcgatg cggtcgtcat aggattcgac ggcaaccttg
      721 gcacgttctc cgggctcacg gacgatcttt ttgatcacta tcaggccgtc gaagatctca
      781 ggcacctcga tttcgagcag cttcgagagg aacagcgggc tcgtgcgcga caaaataatg
      841 accggcgagt tgtttttcag ctccactttc tttaccaccg cgcggatact ttcgcctttt
      901 ttgaaatagt cctgcgggat ctgttcggat ttgggcagga taagctcgct gccgtcctcg
      961 tcaagcagga ggatctcctt tttccagacc tgatagactt ccgcgctgac gatctcgcct
     1021 accctttcgc tatacttctt aacgaggaca ttctttttca ggtccccgat ccggctggca
     1081 agcgtctgtt tcgccgcgag gatcgcgcga cgaccgaaat ccaggatctc cacttcttca
     1141 taaagctcct cgccgacctc atagtcgggc tcgatcttta ccgcgtcagt ataggcgatc
     1201 tccgcgagcg gatcattcac ttccccgtcg ttgacgatcg tcctgcggcg catgatctca
     1261 aggtcgcctt tctcggcatt gacgatcacg tcaaaatttt cgtcgcttcc atattttttc
     1321 cgcaacaaag tcttaaagac gtcttcgacc actttcatca tggtcgggcg gtcgatattc
     1381 tccgcttctt tgaaatcctg gaaagcttcg atcagattga tacttgccat agcactgaat
     1441 tttagaacta gaaaactact tgaattcttg ttgatttaat gttatcgaat aaaaatgcat
     1501 gattcaccgt ctctttcttc ttattgcggc ccctggactc ttcgataatg atcccatcct
     1561 cgctgacttc cagcagtcgg ccgccgtatt tcgagccgtc ctgcaggacc agttcgacca
     1621 gccggccgac gttcttttta tactgcctgt gaagcttcag gggctcgtcg agcccgggcg
     1681 aagaaacttc caacgaaaaa tcccccgggg gaaagatccc tgatgcttcg atcttcttgt
     1741 acaatgcccg gttaaaccgg acgcattttt cgatggagat gccctcgtcg ccgtcaataa
     1801 agaccttcac gttacgggtg ggcttgatgc gcacctctac caggaaatac cccggctctg
     1861 cggtgagcac ctccccgacc aagccttcca ccgttttaat tgcctcactg tccatgtgtt
     1921 ataattatgc ctgggccggc tattaattaa agaagggaac gctgccgttc ccttctactg
     1981 tttcttatcc tttgcaaatg tagggtaatt attttatagt acatttaaaa aaataagtca
     2041 tgggcgaacc tcagaatttg ggcagtttta ttgccggcaa caaggatctg gtcaaggaat
     2101 acctggagac gcggctggag atctaccggc tccagaccgt gcgggtcgtg gccaagtcag
     2161 caggtctggt cgcctggatc tttttgtccc tgctgttggg atttttgttt ttgttttttt
     2221 gcggcctgat gctgggttgc tggtttgcgg gcatatttca cagctacgtc acgggattcg
     2281 gcctggtggc gctgctgatc ctggccgtct tcatactggt cgcgatattc cggcggtcat
     2341 tgttcgtaaa ccccgtgatt caagctatta tccaaaaatc caaggacgga gaataaaaca
     2401 tgggaaaaat gagcaaggcc gcacgaagcc tccaggaact cgacgcagag atcgctgctc
     2461 tcaagcaaaa agcccgccgg ctggaggatg agctggatga aagctgggcc tattttcagg
     2521 aacattccgg ccagatgttt gtccggtcga tcctccccag gtcgctcgaa ggccttgttc
     2581 ccctgaccgg catcagggtc ctggatgcaa tcctgcagag tgaacggctg cagcggatca
     2641 tcgaaaggct ggccggccgg tctgcggcct ggttgggcga cgggctcaat tggctgacga
     2701 acaggatatt taaagacgag ggataacggt ttgcctttaa caccctttta attcttattg
     2761 tcgtaactta gcagactaat ttgttgaaaa atgatgctgg ctgatctgct attcctggtg
     2821 ctggtagggt acctcttata ccgttttatt tatggttttg tcgtaccgtt gatgcgggct
     2881 tcccggcagg tcagagaaca gttccggaac atgcagcgcc cttcgaatga ttcttccgga
     2941 tacgaagaaa gccagcggca ttccggcaca cagcggccgc attccggcgc gcagcagccg
     3001 cgttccggcg gcagcggccc gaaacgggga ttcaaggcgc ccgccgagga ctatatcgac
     3061 tttgaggaga tcaagtaagg agcgggaagt ttcaccactg aaagtccatc aggccaatgc
     3121 ccggtcgcag gaacagccct ctcagccccg cttgttccgc gccttccaca tttacgatcg
     3181 cgtcgtccac aaagagcgtt tcattccctt tcaggttgtt ttcctgcagg atatattgaa
     3241 acgactcggg atccggtttg cgcattccca tgatgtggga gtaataggct ttctcgaaat
     3301 ggtcctcaag cgatccgctt ccaaagctgc gagtccagat ctgccgtagg gcggcgaggt
     3361 gcagggcatt ggtattgctg aaaaggaaaa gccggtaagt tttccgcagc tccgtcaaaa
     3421 ggcggatgcg ctccggcgga aaatccagca gcagggcgtt ccagctatcg acgatggctt
     3481 ggtcggaaac ttgatcaagg gcgccgaaac gccggatgga atggatgaac tcctggtcgg
     3541 tcaacttccc cacttcgtag tcgcggaaaa acgactcggc gaatccatgc ccgaagtatt
     3601 tgcgaaaatc ctttacaccc agcgcggtga aggcgtcttc ggtccgccgg ttatcgaggt
     3661 tgatgatgac gccgcccagg tcaaagatga tgttcttgat cccgatcatg atccaaatat
     3721 attcatttgc ctgaaaggat ggatgggcct gccgctatga tcgaggcagc agcctaaacc
     3781 tgtcatttgg tccagttgac cttttccgtc agcacgtcgc gggaattgcc gccaaccatg
     3841 atcagaaagt cgccgccttc ccagtcgtat ttcaggtcat tggtataata tttcagcatg
     3901 ccggtggtga cggtgaaaga aacctttctc gattcgccgg ctttcagggc gatcttttcg
     3961 aaccccttca attccttgac gggccgggtc atggctccaa ccagttggcg gatatacagc
     4021 tgaacgacct ctctgccatc tacaccgccg tcgttggtaa cggtaacagt ggcggtcagc
     4081 cgctgatttc ccttcaggct gctgctgctc agcgtgaggc cgctatacgt aaaatgcgcg
     4141 tagctgaggc cgtagccaaa cggatagacc ggatcgttcg agacatccag gtagttggaa
     4201 cggaacttct ggaaccattg accttcgagc ggcctgccgg tattcatgtg gttatagtag
     4261 agcgggatct gaccgacgtt ctgcggccag gtggtactta atttgccgga agggttgaca
     4321 tctccgaaaa gcacgtcggc gatagcatgg cccgcttccg ttccgccgaa ccagacattc
     4381 aggatggcgg ggaccgcatc gttttcccat tttatcgcta agggacggcc ggtgaaaagg
     4441 acaagcacaa cgggcttccc cgttttc
//
LOCUS       DATGKE010000044         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1243887, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000044 DATGKE010000000
VERSION     DATGKE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243887"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VKU83_00750"
     CDS             <1..819
                     /locus_tag="VKU83_00750"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HLI92099.1"
                     /translation="PDSLRRSGHSCSCRPMPQDSTAQDSTAQDQTFCSGIHNTAFNAG
                     EVLTYRVYYSVAGAYIAAGDASFTTTLEKFDHKEVYHVVGEGKTFPFEDKFFRVRDRY
                     ESYIDTATLQPYKFIRDVDEGGYKTQENVTFIKSANTAITNTGVYQVPNCIQDVLSAI
                     FYARNIDFSKHKPGDKIPFDMFLDRQVYHLYIRYLGKETIRTKYAKFRTIKFKPLLVK
                     GTMFEGGEKMTVWVSDDPNHIAVRIESPITVGKVSVDMSYYKNLRYPLSSLVDL"
     gene            complement(906..1748)
                     /locus_tag="VKU83_00755"
     CDS             complement(906..1748)
                     /locus_tag="VKU83_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLI92100.1"
                     /translation="MQLYSVPTGYFKLDGGAMFGVVPKTLWSKVQPADENNLCTWAMR
                     CLLIEDAGKLVLVDNGIGNKQDTRFFSHYYLHGDDSLDKSLAAHGFHRDDITDVFLTH
                     LHFDHCGGSIVREGDQLLPAFRKATYWSHSRHWKWATEPNDREKASFLKENILPIQAS
                     GQLKFVDEHGGGDAVLPGLSVRLVNGHTDAMMLPQVACNGRTVVFMADLLPSIHHLPM
                     PWIMAYDMFPLTTLTEKKAFLEEAVTGNYVLFFEHDPAYECCEVTVTNKGIRSRRTFT
                     LAEL"
     gene            complement(1759..>2446)
                     /locus_tag="VKU83_00760"
     CDS             complement(1759..>2446)
                     /locus_tag="VKU83_00760"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI92101.1"
                     /translation="ATIFGDKMLHLDQIKLIVTIVLIQLVAIIGATWMSRLSSRYGNL
                     RVLMGVIVIWIMVCISAYLTAGRAERLELFYVRIAALEQQKEDLLAHRSTMNAVLFDQ
                     EDALIEAGLSRSKLELEPHQQPIEYSFYALALMVGLVMGGIQSLSRSTYSKLMPETRD
                     TASYFSYYDLTEKIAIVIGMGSFGLITQQLGMKNSVISLTVFFVLGLAFLYSALIRNK
                     QTGVIATPVL"
BASE COUNT          582 a          688 c          660 g          516 t
ORIGIN      
        1 cccgattcgc tccgtcggtc cggccactcc tgctcctgcc gtccaatgcc gcaggattca
       61 acggcgcagg attcaacggc gcaggatcag acgttttgct cgggaataca taataccgct
      121 tttaatgccg gcgaagttct gacgtaccgg gtctattatt cggtggccgg cgcctatatt
      181 gccgcaggcg atgcatcctt cacgaccacc ctcgagaaat tcgatcacaa ggaggtttac
      241 catgtcgtcg gagaagggaa gacgtttccc tttgaagaca agttctttcg cgtccgcgac
      301 cgatacgaat cctatattga caccgccacc ctccaaccct ataaattcat ccgggacgtc
      361 gatgaaggcg gctataaaac gcaggaaaac gtcaccttca tcaaatccgc caacacggcg
      421 atcacgaata ccggtgtcta ccaggtgccc aactgtatcc aggatgtgtt aagcgctatt
      481 ttctatgccc gcaacatcga tttcagcaaa cacaagccgg gcgacaagat cccgttcgac
      541 atgttccttg acaggcaggt ctatcacctc tacatccgct accttggaaa ggagaccatc
      601 cggaccaagt acgccaagtt ccggacgatc aaattcaaac ccctgctggt gaaaggaacg
      661 atgttcgaag gcggagagaa aatgacggtc tgggtatcgg atgaccccaa tcacattgcg
      721 gtccgcatcg agagccccat caccgtcggg aaagtttccg tggacatgag ctattataag
      781 aacctaaggt atcccctcag ctcgctggtg gatctgtaag cggagatttt catgcgctat
      841 gagggcgagc tggcgaaccg gcgaaccggc gcgttctgcg ctccgggaat ggaaggataa
      901 ctgatctaca actccgccag cgtgaacgtc cgccggctgc gaatgccctt attggtaacc
      961 gttacctcgc aacattcgta tgccggatcg tgctcgaaga acaatacgta atttccggta
     1021 acagcttctt cgagaaaggc ctttttctcc gtcagcgttg tcagcgggaa catatcgtag
     1081 gccatgatcc agggcatagg cagatggtga atggaaggca aaagatcagc catgaagaca
     1141 accgtccggc cgttacaggc cacctggggg agcatcatgg cgtcagtatg gccgttcacc
     1201 agccggaccg aaagacccgg cagaacggca tcgcctccgc cgtgttcgtc tacgaacttc
     1261 aactggccgc tggcctgtat cggcaggata ttttccttca ggaacgatgc cttttccctg
     1321 tcgttcggct cggtagccca tttccaatgg cgtgaatgcg accagtaagt agctttcctg
     1381 aaagcaggca gcagctgatc gccctcgcgg acgatgctgc ccccgcagtg atcaaaatgc
     1441 aggtgtgtca ggaacacgtc cgtgatatcg tcgcgatgga agccatgggc ggcgagtgac
     1501 ttgtccagcg agtcgtcgcc atggagatag taatgactga agaaacgggt atcctgctta
     1561 ttcccgatgc cgttgtccac gaggacgagc ttgcccgcat cctcgatcag caggcaacgc
     1621 atggcccagg tgcacagatt attttcgtcc gccggctgga ccttgctcca caaggttttc
     1681 ggcacgaccc cgaacatggc gccgccgtcc aatttgaaat agccggtagg gacactgtac
     1741 agttgcatgg catatcgttt agaggaccgg tgtcgcaatc acgccggtct gtttgtttct
     1801 tatcagcgcg gaatacagga atgcgagacc aagcacaaag aacaccgtca gcgagatcac
     1861 cgaatttttc atgccaagct gctgggtgat caggccgaag ctgcccatgc cgatcacgat
     1921 ggcgatcttt tcagtcaggt catagtagct gaaataggag gcggtatccc gggtctccgg
     1981 catcagtttg gaataggtcg accggctcag ggactggatg ccgcccatta ccagtccgac
     2041 catcaaagcg agggcataga acgaatattc gatcggctgc tggtgcggct cgagctccag
     2101 cttcgatcgc gacagcccgg cttcgatgag ggcatcttcc tgatcaaaga gcaccgcatt
     2161 catcgtggag cgatgggcca gcaggtcttc tttctgctgt tccagggcgg caatcctgac
     2221 gtaaaaaagc tccagccgtt cggccctccc ggcggtgaga tacgcggaga tgcaaaccat
     2281 gatccaaatg acaataacgc ccatcaggac ccgcaggttg ccataccggg aagaaagccg
     2341 gctcatccac gtcgcgccga tgatggcgac gagttggatg agcacgatcg taacgatcag
     2401 cttgatctga tcgagatgca acatcttatc gccaaaaatg gtcgcg
//
LOCUS       DATGKE010000045         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1227890, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000045 DATGKE010000000
VERSION     DATGKE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227890"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VKU83_00765"
     CDS             complement(<1..1191)
                     /locus_tag="VKU83_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLI92102.1"
                     /translation="MKSPFRKLIRISVVLFVLVLLLNFFGYYINHIRSLENKELIEAI
                     NRSSHQQTLSQRIAKQTILMLDHPEQVRQDASTDTLARLIDIFEANQNMLVQQVNATP
                     TPVPQKVFQIKLLLSTAQPYFETITAISREVSQADSAMLVINKKMYLRDLLAGESRYN
                     GMMSEVNTHYSEMVQEKSSEVATIDTTKLVSLIAAIIGLIILVLEPAFKKGERNYKEL
                     QKARNDLLNEKKFLASILQSQTNYVIRINRSGHFTYANPAFLKTFGYTEQEVQNILFY
                     TTIFPKDLARCQQVAEQCWNNPGKVFRLVIRKPIGHTRQFLWTEWEFLALADENGRIE
                     EIQGIGSDISEKLQMEQGKEEAIRTLSYAMTYAKMGSWKLDLISGELALSKEFKALLA
                     MDAED"
     gene            complement(1290..>2046)
                     /locus_tag="VKU83_00770"
     CDS             complement(1290..>2046)
                     /locus_tag="VKU83_00770"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92103.1"
                     /translation="FRRLKQSYPALKLHTLGPPEVAHICKLEKMSHRDVLTELRAAGM
                     DSLPGAGAEILVDRVRRLISKGKCGAQEWLDIMAEAHQLRITTSATMMFGHVETIPER
                     MEHLIRLRDVQSRKPADAKGFLAFIPWTFQDVDTLLARIRGVHNLVTPEEYIRMIAIS
                     RIMLPNILNIQASWLTVGRQTAQICLHAGANDFGSIMLEENVVSAAGAPHRFTARGIQ
                     AAIREAGFEPQLRNQQYEWRELPAEMEEQIITY"
BASE COUNT          403 a          532 c          613 g          498 t
ORIGIN      
        1 atcttcggcg tccatggcga gcagggcttt gaattccttg ctgagcgcca gttcgccgct
       61 gataaggtcc agtttccagg accccatttt ggcataggtc atcgcgtagg acagggtcct
      121 gatcgcttct tccttgccct gctccatctg cagcttttcc gaaatatccg atccgatccc
      181 ctggatctcc tcgatgcggc cgttctcatc ggccagcgcc aggaactccc actccgtcca
      241 aaggaactgg cgggtatggc cgataggctt gcggatgaca aggcggaaga ccttcccggg
      301 gttgttccag cactgttcgg cgacctgctg gcatcgcgcc aggtctttcg ggaaaatggt
      361 ggtatagaaa aggatgttct gaacctcctg ctccgtatag ccgaaagttt tcaggaaggc
      421 cgggttggca tacgtgaaat gaccgctccg gttgatgcgg atgacgtagt tcgtttgcga
      481 ctgcaggatg gaggcgagga atttcttttc attcagcagg tcgttgcggg ccttttgcag
      541 ctccttgtaa ttcctttcgc ccttcttgaa ggcgggctcg aggacgagga tgatgaggcc
      601 gatgatggcg gcgatcaggg agaccagttt cgtagtgtcg atcgtggcga cctcgctgct
      661 tttttcctgg accatctccg aatagtgcgt attgacctcg ctcatcatcc cgttgtaccg
      721 gctttctccg gcgagcaggt cccgcaggta cattttctta ttgatgacca gcatcgccga
      781 atctgcctgg gaaacctccc gggagatggc ggttatcgtc tcaaaatagg gctgggcggt
      841 ggaaagcagg agttttatct ggaagacctt ctgcgggaca ggggtgggag tggcattcac
      901 ctgctgcacc agcatattct ggttcgcttc aaagatgtcg atcaatctgg cgagggtgtc
      961 ggtcgatgca tcctggcgca cctgttcggg atgatccagc atcaggatgg tctgcttggc
     1021 aatgcgctgg ctcaaagtct gctggtggct ggaacggttg atggcttcga tcaactcctt
     1081 gttttccagg gaccttatat ggttgatata atacccgaag aaattcagca gcaggacgag
     1141 aacgaacagg acgaccgaga tgcgaattaa ttttcgaaag gggcttttca tgaatgtggc
     1201 taggatcttc cctataggaa cgtaaaatcg aattattaag tttctggaac gggcaaaaaa
     1261 agcggggcta aatgactacg tgttgagtgc taataagtta tgatctgttc ctccatttcg
     1321 gcaggcaact cgcgccattc gtactgctgg ttgcggagct gcggttcgaa ccccgcttcc
     1381 cggatcgccg cctggatgcc ccgggcggta aaacgatggg gggcgcctgc tgcactcaca
     1441 acgttctctt ccagcataat actgccgaaa tcattggctc cggcgtgcag gcagatctgg
     1501 gcggtttgcc ggcccaccgt cagccagctt gcctgaatat tcaggatatt gggcagcatg
     1561 atgcggctga tggcgatcat ccggatatat tcctccggag taaccagatt gtgaacgccg
     1621 cgaatgcgcg caagcagcgt gtccacatcc tggaatgtcc agggtataaa ggccaggaaa
     1681 ccttttgcat cggccggctt gcggctctgg acgtcacgga ggcggatcag gtgttccatc
     1741 cgttccggaa tggtttcgac gtgcccgaac atcatggtgg cggatgtggt gatccggagc
     1801 tgatgggctt ccgccatgat atcgagccat tcctgggccc cgcacttgcc tttgctgatg
     1861 aggcggcgga cccggtcgac gaggatttcc gcgccggctc ccgggaggga gtccatccct
     1921 gccgcacgca attcggtcag gacgtcccgg tggctcattt tctccagttt gcagatatgc
     1981 gctacttcgg gcggcccgag ggtatggagt ttcagggcag ggtaagattg cttcaggcgg
     2041 cggaac
//
LOCUS       DATGKE010000046         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1537731, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000046 DATGKE010000000
VERSION     DATGKE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537731"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VKU83_00775"
     CDS             complement(<1..1430)
                     /locus_tag="VKU83_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HLI92104.1"
                     /translation="MRLRLTLILLMLSGALWAGPIVLISPDSGLKFIFRLAKGGTPEY
                     DVVYNGVQLVAHSPLSLRFAGSGGFGRNLVARPAAVTAGADDYTLVVGKTRSVHDRYR
                     EVLIPLEERNGKAAGPPGSLSSAPRRARKVDLEVRVFNDGLAFRWVIPAQEGWDSINL
                     VDEGSCFNLVGDPLLMATYREGFTTSHEGLYTNRRFSAVTDDTLMDMPVFLDFDGAAG
                     VVSGKPGKGAAARAEGSPHIYAAITEARLVDYAGMYLVKHGGLLKSELSPYPGQPGGP
                     VPSTGPIGAGGAVDHEPGTAADLESGRKTAVRVRATLPHSSPWRVMLVGSRAGALIES
                     NVITDLNDPCAIKDVRWIKNGKTDFHWWNGDVVADTGFQPGINFESNKYYIDFCARNH
                     IDYHTVIGWSGIAWYTNDGVSYDAGPHSDVTRPIPGLDMKQICDYAHSKGVGIRVWVH
                     WRALYPKIDSAFAIFQRWGVSGMMVD"
     gene            1494..1835
                     /locus_tag="VKU83_00780"
     CDS             1494..1835
                     /locus_tag="VKU83_00780"
                     /inference="COORDINATES: protein motif:HMM:NF042904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6799 domain-containing protein"
                     /protein_id="HLI92105.1"
                     /translation="MKYLILCLALALSLNTRAQDSTAGTAPRDSTRTHLSKPWDYIMM
                     KHGQLIEVTKGVQKPVTADVILVNETTIHPDGTINDSNGKTKHLREGKYITMDGRIRN
                     LNRLPEGLPPR"
     gene            complement(1879..>2247)
                     /locus_tag="VKU83_00785"
     CDS             complement(1879..>2247)
                     /locus_tag="VKU83_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92106.1"
                     /translation="MAGFSMLTGLIVLIASVLVSKFQRIQESVLLRTLGASRRQVLVI
                     TMLEYFFLGGLASVVGIALSMGFTGLLAKFAFDGVFVPNGWVAGALFIGVSSLTVLIG
                     LYNVRGVLNRPPLEVLRREG"
BASE COUNT          510 a          754 c          567 g          416 t
ORIGIN      
        1 aagtccacca tcatgccgct cacgccccag cgttggaata tcgcaaacgc gctatcgatc
       61 ttcgggtaaa gcgccctcca gtgaacccaa acccggatgc cgaccccctt gctgtgggca
      121 tagtcgcaga tttgcttcat atcgaggccc gggatgggcc tggtcacatc ggagtgcggt
      181 ccggcgtcat agctgacgcc gtcgttcgtg taccaggcaa tccccgacca gccgataacg
      241 gtatggtaat caatatgatt cctcgcgcag aagtcgatat aatacttgtt cgactcaaaa
      301 ttgattcccg gctggaagcc ggtatcggct acaacgtcac cgttccacca atggaagtcc
      361 gtcttgccat ttttgatcca acgcacatcc ttaattgcgc agggatcgtt caggtcggta
      421 atgacgttcg attcaatcag cgctccggcc ctcgaaccca ccagcatcac cctccaaggc
      481 gacgaatggg gcagcgtggc cctcaccctg actgctgtct tgcgccccga ttccaggtct
      541 gccgcggtgc cgggctcatg atccaccgcc cctcccgccc caatcggtcc tgtcgacggc
      601 accggtccgc cgggctggcc ggggtaaggc gacaactcgc ttttaagcag gccgccgtgt
      661 ttgacgaggt acatgccagc gtagtcgacc agccgcgcct ccgtgatggc agcataaatg
      721 tggggactgc cttctgccct cgccgcggcg ccttttcccg gctttccgct aactaccccg
      781 gcggcgccgt caaagtccag gaacaccggc atatccatga gcgtatcgtc ggtgacagcg
      841 ctgaaccgcc ggttcgtgta cagcccttca tgtgaagtcg taaatccctc tctgtaagtc
      901 gccattaaca atggatcgcc caccaggttg aaacagcttc cctcatccac caggttaatg
      961 gagtcccatc cttcctgcgc cgggatcacc cagcgaaacg ctaacccgtc gttgaagacc
     1021 cgcacttcaa ggtcgacctt cctggcgcgc cgcggcgctg aagacaggga tccgggcggg
     1081 ccagcggcct tcccattccg ctcttccaat gggatcagca cctcccggta ccggtcatgc
     1141 accgaacggg tcttccccac aacaagcgta tagtcgtccg ctcccgccgt gactgccgcg
     1201 gggcgcgcaa ccaggtttcg cccaaacccg cccgatccgg caaaccttag actcaagggc
     1261 gaatgggcca ccagttgcac tccgttatag accacgtcat attcgggcgt cccgcctttt
     1321 gccaaccgaa aaatgaactt cagtcccgag tccggcgaga tgaggacgat ggggccggcc
     1381 cacaaggcgc ccgacagcat cagcaaaatc agcgtcaatc gcagtctcat aagcgtcaat
     1441 tttcgataaa gataagaggc aagggaattg catagccaac cgaaagccgc cacatgaaat
     1501 atcttatact ttgcctggcc cttgccttaa gcctaaatac ccgggcccag gacagcaccg
     1561 ccggcacggc tccgcgcgac agcacccgga cgcatctttc gaaaccctgg gattatatca
     1621 tgatgaaaca cggccagctg atcgaagtca ccaaaggcgt ccaaaagcct gtcaccgccg
     1681 acgtcatcct tgtcaatgag acgaccattc accccgatgg gaccatcaac gattcgaatg
     1741 gaaaaacaaa gcatttaagg gaaggcaaat atattaccat ggacggccgc attcgcaacc
     1801 tgaacaggct ccccgagggc cttccgccgc gatagctgcg cgcttaatgc tcgcggtggc
     1861 gctcttcgcg ggccgccctc agccctcgcg ccgcaggact tccagcggtg gccggtttaa
     1921 cacgccgcgg acattgtata atccaatcaa cacggtcagc gaactcaccc cgataaacaa
     1981 cgctcccgcg acccatccat tcggaacaaa aaccccatcg aaggcaaact tcgccaataa
     2041 ccccgtaaag cccatcgaca aagcgatccc aaccaccgag gccaacccgc cgaggaaaaa
     2101 gtactcgagc atcgtgatga ccagcacctg ccgcctgctc gcacccagcg tgcgcagcaa
     2161 cacgctctcc tggatccgtt ggaatttgct gaccagcacg ctcgcgatca gcacgatgag
     2221 gccggtcagc atactgaagc cggccat
//
LOCUS       DATGKE010000047         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1334587, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000047 DATGKE010000000
VERSION     DATGKE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334587"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /gene="pheT"
                     /locus_tag="VKU83_00790"
     CDS             complement(<1..2250)
                     /gene="pheT"
                     /locus_tag="VKU83_00790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLI92107.1"
                     /translation="MTISYNWLCEYLPVTVQPEKLSHILTAVGLEVESMERFESVKGG
                     LRGLVIGEVLTCGPHPNADKLKLTLIDAGGAQPQPIVCGASNVAAGQKVVVAVPGTTI
                     YPMAGDPVTLKVATIRGVESHGMICAEDEIGLGSSHAGILVLPADAPVGMAAADWFQP
                     FSDIVFEIGLTPNHMDAMSHLGVARDVCAWLAHHENGDFRPRQPSVDAFRVDSTKLPV
                     EVRILNPRSCERYSGVSIAGIRIGESPRWLQDRLRSIGVRSINNIVDVTNFVLHEMGQ
                     PLHAFDWDRIRGQKIIVRNLPEGTPFVTLDEKERKLAADDLMVCDEGGGIAMAGVFGG
                     LHSGISASTENIFLESAWFNPTDIRRTSFRHGLRTDAAVHFEKGMDISGTVRALKRAA
                     LLIKELAGGEIASEIVDACPGPPRRPVVALRNEYLKKLSGKEYGRDTTKKILLSLGFE
                     IAAETPEELEIAVPAHKPDITLAADIVEEVMRIDGLDNIPIPPALTISPSVENHPTWA
                     AWKLEAGSWLVGQGFNEILTNSITNSAYFNEAELASGVKMINNLSAELDIMRPSMLET
                     GLESVAYNLNRKNSSIRFFEFGKTYRSHGIGQYEETNHLCLYLTGQLAENNWKGKGVP
                     VDIFYLKGLCEKIFAVAGLRFPGWSGKQDPKLLTGLAVRSDGEIVAEAGEVDPGILRR
                     FDCRQPLFYADIRWDALMEMASRNRIEFRELPRQLPVYRDLAMIVRKSLPYEKVEDAI
                     RGTRLDKLRE"
     gene            2562..4211
                     /locus_tag="VKU83_00795"
     CDS             2562..4211
                     /locus_tag="VKU83_00795"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HLI92108.1"
                     /translation="MRKYLNALIASLLLLSQFSCGPTHIVVESPPPPQPAEPAPPPPE
                     TSYQAFYDALSPYGEWINDPEYGYVWMPNAGPDFKPYATNGNWVYTDEGWTWASNYPW
                     GWAAFHYGRWFYEEGYGWMWIPGNEWAPAWVSWRRSPEYYGWAPLGPSVGMDVAAGGG
                     YNPPAHYWNFVPQQYVASPRITNYYVNERQNVTIINNTTIVNYRESNTRVNNYVGGPD
                     PREVSRVAGMELRAVAVRESNTPGEHHGDGAYAIYRPRINAAPQGNGGMAGRPAPARV
                     QTINNIRPVNPTIYTNNGRYNNIRRLDNNANNLPGNHADGNGNPVNTGNGYHDARDND
                     GRNNDARNNDPRNNDPRNNDARNNDGRNNDPRNNDARGNDNRNNREPGSPPAVQPYRP
                     PGSQPFNRPDNQPGNQPSGQPANQPSPQPQPNNVRPVTLPFMRQQNPNNARPVTQPYT
                     QPQAPNNARAGSQGNAQPANPPAGPNRNNLNGGSQPKPAANPGSVAPNGQRNYGNGNN
                     ANGRPGRPGGNANGRKPASNQGQPAPDNRHKPDSANRDHHS"
     gene            4705..>5453
                     /locus_tag="VKU83_00800"
     CDS             4705..>5453
                     /locus_tag="VKU83_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HLI92109.1"
                     /translation="MDLTNLNLNKDRNKLRRKSSIDLGTMVYGKIPPQAKELEEAVLG
                     AIMLEKSAFDTVVEILKPECFYLEGHQRIYRAMQGLAQKSQPIDILTVVEELKFRQEL
                     EMIGGPYYVTKLTNAVVSAANIETHSRIILQKFIQRELIRVSGEIIGDAYEDSTDVFD
                     LLDDAESKLFEITNNHLRKNFDSIDTVLVKTIQRIEDLRHKQEEITGVPSGFPSLDRV
                     TYGWQSTDLIILAARPAVGKTAFALNLARNAA"
BASE COUNT         1227 a         1680 c         1455 g         1091 t
ORIGIN      
        1 ttcgcgcaac ttgtccaacc gcgtcccccg gatcgcatcc tccacctttt catacggcaa
       61 cgattttcgg acgatcatcg ccaggtcgcg gtataccgga agctgccgcg gcagttccct
      121 gaattcgatc cggttccggg atgccatttc catcaacgca tcccagcgga tgtctgcata
      181 gaacaggggc tggcgacaat cgaaccgccg caggatcccg ggatccacct cgcctgcctc
      241 cgcgacgatc tcgccgtccg atcgcacggc caggcctgtc aacagctttg gatcctgctt
      301 gccgctccag ccgggaaagc gcagccccgc gaccgcaaat attttttcac acaatccttt
      361 caggtagaaa atatctaccg gaacgccctt tcccttccag ttattctccg caagctgacc
      421 ggtcaggtaa aggcagagat ggttggtctc ctcatactga ccgatgccat gcgaccggta
      481 ggttttgcca aactcaaaga aacggatgct gctgtttttg cggttcaggt tgtaggcgac
      541 cgattcgagt cccgtctcca gcatcgacgg ccgcatgata tcgagttccg cgctgagatt
      601 gttgatcatc ttcacacccg aggccagctc cgcttcattg aagtacgcac tgttggtgat
      661 cgaattggtg aggatctcgt taaagccctg acctacgagc caactaccgg cctccagctt
      721 ccaggcagcc caggtgggat ggttctccac ggacggcgag atcgtcagcg ccggcgggat
      781 gggaatattg tccaacccat cgatccgcat cacctcttcg acgatatcgg cagcgagtgt
      841 gatgtccggc ttatgtgcgg ggacggctat ttccaattct tcgggtgtct ccgccgcgat
      901 ctcaaagccc aggctgagga ggatcttctt cgtggtatcc cggccgtatt ccttgccgct
      961 cagtttcttc aggtattcgt tgcgaagcgc gaccaccggt cgccggggag gcccgggaca
     1021 ggcgtccacg atctccgatg cgatctcgcc gcctgccagc tcttttatca gtaaggctgc
     1081 ccttttcaat gcacggaccg tgccggaaat atccatgccc ttttcgaaat gcacggcagc
     1141 atcggtccgc agcccgtggc ggaacgaggt ccggcggata tccgtcggat tgaaccacgc
     1201 gctctcgagg aaaatgtttt cggtagatgc gctgatcccg ctatgcagcc cgccgaagac
     1261 tccggccatc gcgatgcctc ctccttcgtc gcacaccatc agatcgtctg ccgccagctt
     1321 cctttccttt tcatcgaggg tgacaaaggg ggtgccttcc ggaaggttgc ggacaatgat
     1381 cttttgtccc ctgatccggt cccagtcgaa ggcatgcagc ggctgtccca tttcgtgaag
     1441 cacgaaattg gtaacgtcga cgatattgtt gatcgaacgg acgccgatgg atcgcaacct
     1501 gtcctgcagc caccgcgggg actccccgat ccggattccc gcgatgctca ctcccgaata
     1561 acgttcgcag gaccgggggt taaggatccg gacctccacg ggcagttttg tggagtcgac
     1621 ccgaaaggca tccaccgaag gttgccttgg ccggaaatcg ccgttctcgt ggtgggcgag
     1681 ccatgcacaa acgtcccgcg ccacgcccag gtggctcatg gcatccatat ggttcggcgt
     1741 aaggccgatc tcgaacacaa tgtctgaaaa aggttgaaac caatccgccg cagccatgcc
     1801 cacgggagcg tcggccggaa gcaccaggat ccccgcatgg ctgctgccga gcccgatctc
     1861 atcctcggcg cagatcatgc catggctctc gacaccgcga atggtcgcga ctttgagggt
     1921 gacgggatcg cccgccatgg gatagatcgt ggttccgggg accgcaacca ccactttttg
     1981 ccccgccgcc acattactgg cgccacaaac gataggttgc ggctgcgcgc ctccggcgtc
     2041 tatcagcgtc agcttcagct tgtcggcatt cgggtgcggc ccgcaagtca gcacctcgcc
     2101 gatcaccagt ccccgcaggc cgccttttac cgactcgaaa cgctccatgc tttccacttc
     2161 cagcccgacc gcagtaagaa tatgcgaaag cttttccggc tggacggtca ccggcaggta
     2221 ttcacaaagc caattgtaac tgatggtcat gacgggatgc gggtatttga ttggatggca
     2281 aagataatcg ttcccggtca tctccggagg agggaccggg cggcaggagg ccgccgcccg
     2341 gccgccgcgg caccgcctgc agtaccgaaa taattcgccg ggaacccgag aaatccggcc
     2401 ggctgaaaaa caattttccc ccgccgtcgt tctttccata gaaagtatcc aaatccctta
     2461 cctatttgtg aatttttgac aatcaattgt taatatgtta aaacggctgg taatttgcac
     2521 gattcctgcc ccataactag cgaacactta aaactcctcc tatgcgtaaa tatttaaacg
     2581 cgctgatcgc ttccctttta cttcttagcc agttttcctg cgggccgacc catatcgtgg
     2641 tagaaagccc tccgccgccg cagccggccg aacctgcccc gccgccgcca gagacgtctt
     2701 atcaagcgtt ctacgacgct ctcagcccct acggcgaatg gatcaacgat cccgagtacg
     2761 gttatgtctg gatgcccaat gccggcccgg atttcaaacc ttatgctacc aatggaaact
     2821 gggtttatac cgacgaaggc tggacctggg cgtcgaatta tccctgggga tgggccgctt
     2881 tccattatgg acgctggttc tatgaggaag gctacggctg gatgtggata cccgggaacg
     2941 aatgggctcc cgcctgggta agttggcgcc gcagccccga atattatggc tgggccccgc
     3001 tcggacccag cgtcggcatg gacgttgcag ccggcggcgg gtacaacccg cctgctcatt
     3061 actggaattt cgttccccag caatatgtcg ccagtcctcg tatcacaaac tactacgtca
     3121 acgagcggca gaatgtgacg atcatcaaca acacgaccat cgttaattat cgtgaaagca
     3181 atacccgtgt caataattat gttggcgggc ccgatccccg cgaggtttcc cgggtcgcgg
     3241 gcatggagct ccgcgcggta gccgtgcggg agagcaacac cccgggtgaa catcacggtg
     3301 acggcgccta tgccatctac cggccgagga tcaacgccgc gccacaggga aatggcggga
     3361 tggcgggccg cccagctccg gcgcgggtgc agacgatcaa caatatccgc ccggttaacc
     3421 cgacgatata cacgaataac ggccgttaca acaatatccg caggcttgac aacaacgcga
     3481 ataatcttcc gggcaaccat gccgacggga atggcaatcc ggtgaatacc gggaacggct
     3541 accatgacgc ccgcgacaat gacggcagga acaacgacgc ccggaacaac gatcccagga
     3601 ataatgaccc ccggaacaat gacgcccgga acaacgacgg caggaacaac gatccccgga
     3661 acaatgacgc ccgcggcaac gataaccgga acaaccggga acccggcagt ccgcctgctg
     3721 ttcagcccta tcgtccgccc ggttcgcaac cttttaaccg gccggataac cagccgggaa
     3781 accagccctc aggccagcct gcgaaccagc catccccgca accgcagccg aacaacgtcc
     3841 ggccggtcac gctgcctttc atgcggcagc agaatccaaa caacgcccgg ccggtcacgc
     3901 agccttacac ccagccgcag gcgcccaaca acgcccgggc cggcagccag ggtaacgcgc
     3961 aaccggcgaa tccgcccgcg ggcccgaacc ggaataatct caacggtggc agccagccga
     4021 aacccgcggc taatcccggt tctgtcgctc ccaacggaca gcggaactat ggtaatggca
     4081 acaacgccaa cggacgtccg ggtcggcccg gcggcaatgc caacgggcgc aaacccgcgt
     4141 ccaatcaagg tcagccggct ccggataacc gtcataaacc tgactcggcc aatcgggacc
     4201 accattcctg atccggcctg agcgggcggg gcaacgtatt aaacgaaggc cggcactgga
     4261 ttttacccag cagccggcct ttccttttgc gttccggcag ctgcaggatg ccgcgaaacc
     4321 tcattctgta gttttcatat cccggcaaaa tatttaccgc taattctttc tgcacaggcg
     4381 gtgtgtattt ttccgacgat acgattgaaa acttttttaa attgtggata gcttctcggg
     4441 tactgtgtgt aactatcgga gatcggtgga taaacaagtg aacttctata ggaaaaatat
     4501 agaaaaagaa ttttctcggc ggtaaagatc aggacaccta tattcgccgt cctgactcaa
     4561 agggataaca ttctgttcat gttgccgtaa caatatctta ataaaagcac cgactgaaga
     4621 gaacctcccc gatgagccgg tcccccttgg ggcattagcg gctggacacc ctatttttta
     4681 acgtttctgg acggtttatt gaatatggat ctcactaatc tgaacctgaa caaggatcgc
     4741 aacaagctga ggcgtaaatc ttctattgac cttggcacga tggtttacgg taaaataccc
     4801 ccgcaggcaa aagagcttga agaagctgtc ctcggcgcca ttatgcttga aaagagcgcg
     4861 tttgacacgg ttgttgaaat tctgaaaccc gagtgctttt acctggaagg ccaccagcgg
     4921 atttaccggg caatgcaggg cctggcccaa aaaagccaac cgatagatat cctcactgtc
     4981 gtcgaggagc tgaagttcag gcaggagctg gagatgatcg gcggcccgta ttacgtcacg
     5041 aagctaacca acgccgtcgt gtccgcggcc aacatcgaga cccattcccg gatcatcctg
     5101 cagaaattca tccagcggga actgatccgg gtcagtggcg agatcatcgg cgacgcctat
     5161 gaggacagca cggacgtatt cgacctgctg gacgatgcgg aaagcaagct cttcgagatc
     5221 acgaataacc acctccgcaa aaacttcgac agtatcgata cggtcctcgt caaaaccatc
     5281 caacgcatcg aggatctgcg gcacaagcag gaagagatca ccggagtccc gagcggattc
     5341 ccgagcctgg accgggtcac ctatggttgg caatcgaccg acctgatcat cctcgccgcg
     5401 cgacccgccg tcggtaaaac ggcctttgcg ctcaacctgg cccggaacgc cgc
//
LOCUS       DATGKE010000048         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_817176, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000048 DATGKE010000000
VERSION     DATGKE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817176"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1587)
                     /locus_tag="VKU83_00805"
     CDS             complement(133..1587)
                     /locus_tag="VKU83_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLI92110.1"
                     /translation="MQKNAHRARSAEPLYLQIAAGLQKMIGDEILKIGDKLPSVRKLS
                     EERGISMGTAFQAYYHLEAKGLIEARPKSGYYVRFNMKHMPGLPQVADPEPVESEVSV
                     QEMIATVYSDISAADLVNFSISAPPLSLLPAAKLKKSMLHALRHSRNQGVLYEHIQGN
                     AELRRQLARHAFNWGGKFSAGDIVVTAGCMEALVMCLKAVTRPGDTVAIENPTFFGIF
                     QVIESLGLKALEIPTDPVTGIDLNQLRAAIERFPIKACVFVPTFSNPLGSCMPDKNKA
                     ALVRLITENRIPLIEDDIYGEMYFGAHRPRTCKSFDKEGWVLYCSSLSKSLAPGYRIG
                     WAIPGRFTREVSMTKMMHTVTGTTLTQEAIAHFLAIGRYDFHLKKLRKALHTQCLRYL
                     QGIMTWFPADTKVSRPQGGFVLWVELPRQLNAYRLYQQALKNQVSVAPGQLFSARGQF
                     RNCIRLSYARPWDEEVESAIRTLGNLIKSQIRAR"
     gene            complement(1860..>2334)
                     /locus_tag="VKU83_00810"
     CDS             complement(1860..>2334)
                     /locus_tag="VKU83_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92111.1"
                     /translation="LPLSLSFTDTADLQFVRIKLPRDAIVNSVEEFFDENYSTRNPDE
                     IILYGYSHVKDKENTSGTIAEFYDGKLQSDINRPIGFRESNGKIDWVNYLVTTSPRMF
                     HGMSGSPVFFIFNCTNRKESKIVLGGICSQSNDSLNDGLIVKAGEIIRQLKGHKY"
BASE COUNT          578 a          594 c          583 g          579 t
ORIGIN      
        1 cgaaaccggc gaaatgacgc cgaaactgag cctccgccga aaagtcgtga cggaaaaatt
       61 ccgtgacacg atcgagaaga tctacaacaa cgcctagcgc cgccaatccg ccccttgagc
      121 ttcgtatgcc gcctacctcg cgcggatctg gctcttgatg agattcccca gggtcctgat
      181 ggccgactcg acttcctcat cccatgggcg cgcatagctc aggcggatgc agttcctgaa
      241 ctggccgcgg gcggagaaga gctggccggg cgcaaccgag acctggttct tcaaagcttg
      301 ctgataaagg cgataggcgt tcagctgccg cggaagctcc acccacagga caaatcctcc
      361 ttgcggccgg gaaaccttgg tatccgccgg aaaccacgtc ataatgccct gcagataacg
      421 caggcattgc gtgtggagcg ctttgcgcaa ttttttcaaa tggaagtcat accggccgat
      481 agcaagaaaa tgcgcgatcg cttcctgcgt gagggtcgtg ccggtaacgg tgtgcatcat
      541 tttggtcatg ctcacctcgc gcgtaaaccg gcccggtatg gcccagccga tccggtaccc
      601 aggcgcaagt gacttggaga gcgagctgca atacagcacc cagccttcct tatcgaagga
      661 cttgcaagtc ctcggccgat gggcgccgaa atacatttca ccatagatat cgtcctcgat
      721 cagtggaatc cggttttcgg tgatgagcct gacgagtgcc gctttgttct tatccggcat
      781 gcacgagccg agggggttgc taaatgtcgg aacaaaaacg cacgctttaa tgggaaacct
      841 ctcgatcgct gcgcgcagtt ggttgaggtc gataccggtc accgggtcgg tcgggatctc
      901 caatgctttc aggccaaggc tctcaataac ctggaaaatg ccaaagaacg tcggattctc
      961 gatggctacc gtgtcccccg gccgggtaac ggccttcagg cacatgacca gcgcctccat
     1021 acagcctgcc gtcacgacga tgtcgcctgc cgagaacttt ccgccccagt tgaaagcatg
     1081 acgcgccagc tgacgccgca attcggcgtt gccctggata tgttcataaa ggacaccctg
     1141 gttgcgcgaa tggcggagcg catggagcat ggattttttg agttttgcgg ccggcaacaa
     1201 gctgagcggc ggcgccgaga tggagaagtt cacgaggtcc gcggcgctga tatcgctata
     1261 gaccgtggca atcatttcct gcacgctgac ctcgctttca accggctccg gatcggcgac
     1321 ctgcggaagc cccggcatgt gtttcatatt gaaacggaca tagtaaccgg atttggggcg
     1381 ggcttcgatc aggccctttg cttccagatg ataataagcc tggaaggcgg tgcccatgct
     1441 gatcccgcgc tcttcgctga gcttccggac ggagggcagc ttgtccccga tcttgaggat
     1501 ctcgtcgcca atcatctttt ggagaccggc tgctatttgg agatacaacg gctcggcgct
     1561 gcgcgcgcgg tgagcatttt tttgcatgga tagtagttat agcaatggta atgtggtcat
     1621 ttgaatttaa taataaaaaa actccaagtt tattgcttcc tgagcggtca agcagcggat
     1681 tttactatat cttcctacga gggttgactg taaaaggatg taaattagct tttttgctac
     1741 tgatattttt aggcatttca gtagggtgca agacgcatac tactaaatta aaacactcaa
     1801 gagagaggaa agacacctta tcgttaaaga tatatccaaa aacaggttgg tcttattaat
     1861 taatatttat gtcctttaag ttgcctaatt atctctccgg cttttacgat caacccatca
     1921 ttcaaactat cattagattg ggaacaaata ccacccaaca caattttact ttcctttcta
     1981 tttgtacaat tgaatatgaa aaatacagga ctcccagaca taccatgaaa catcctaggt
     2041 gacgttgtaa ccaaataatt aacccagtca attttgccgt tagattccct aaagcctatt
     2101 gggcgattta tatcggattg caacttgcca tcgtaaaatt cagcgattgt accgctggta
     2161 ttctctttat ctttaacgtg tgaataacca taaagaataa tttcatctgg attacgggta
     2221 ctatagtttt catcaaagaa ttcctcaacg gaattaacta tggcatctct tggtaattta
     2281 attctgacaa actgtaaatc tgcggtatct gtgaaagata ggcttagtgg caat
//
LOCUS       DATGKE010000049         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_402615, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000049 DATGKE010000000
VERSION     DATGKE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402615"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VKU83_00815"
     CDS             complement(<1..463)
                     /locus_tag="VKU83_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92112.1"
                     /translation="MTIPKDDPIVSLYRDSFPDFARIVRRMGGTLEQAKDSFHDALLI
                     YLEKEKAGKLHPHSSPKAYLLGTAKICWLHSLNRGPVLSFPEGIELSEPEDADMPERE
                     RGLLESLVRSGKKCMELLKAFYYDHSSMQDIATRFGFNGRRSATVQKYKCLE"
     gene            641..1729
                     /locus_tag="VKU83_00820"
     CDS             641..1729
                     /locus_tag="VKU83_00820"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLI92113.1"
                     /translation="MPKLTIARYAVIPLLALVALSSYSQPISTPRTPSPAASVSQTVG
                     ISTITVNYSRPSVKSRKVWGELVPYGWNKQGFGAGNDAPWRAGANENTVLRLSHDATI
                     EGHPVPAGEYGLFFVINQDNTGEVVLSKDSRSWGSFWYDPQHDEMRAKITLHAIPQTE
                     MLTYDFQNITRNSAELALNWEKVQFPVKIEFAVDDIVIANAAEELKGPTGFNWQGYAS
                     AANYAVQNKIGYDKALLWIDQAIAMNSNFTTLKIKSDLLRATGKTPEAESMLKDATAV
                     ATEVELNQYGYQLLGNNQQDKAIEIFVLNTQRHPKSANVFDSLGEAYALKGDKKNAVI
                     NFKRSLSLNPPANVRANSEKYLKQLGEM"
     gene            complement(1742..2473)
                     /locus_tag="VKU83_00825"
     CDS             complement(1742..2473)
                     /locus_tag="VKU83_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLI92114.1"
                     /translation="MPVIIICFFATLVRSTFGFGESLVAVPLFALFIPLPVAVPLSVL
                     ISVLVALIVVVQDHRQIHVSSAKWLILYALLGIPIGLMILIYGNAYWVKIGLGVLIIG
                     YSFYTILGKASLALAQDSRPWLFICGFLSGVFGGAYGLNGPPLVVYGNMRKWSPTHFR
                     ATLQAYFLPASLFGIAGYALKGLMGRELFTYFLVCLPAVLPAIFLGRFFNRRLQGSSF
                     FTYVHWGMMIIGLLLILFTVSGLTT"
BASE COUNT          684 a          679 c          676 g          520 t
ORIGIN      
        1 tctccagaca tttatacttc tggacggtgg cggacctacg gccgttaaaa ccaaatcgcg
       61 tggcaatatc ctgcatggag gaatggtcat aatagaacgc tttcaggagt tccatacatt
      121 tctttccgct cctgacaagg gactccaata ggcctctttc tctttcgggc atatccgcat
      181 cctccggttc ggacagttcg ataccttcgg ggaacgataa taccggaccc ctgttcagcg
      241 aatgcagcca gcagatcttt gcggtgccca ggaggtaagc cttgggggag gagtgtgggt
      301 gcagcttccc tgctttttcc ttttccaggt agatgagcag cgcgtcgtgg aagctgtcct
      361 ttgcctgttc cagggttccg cccatccgtc tgacgatccg ggcaaaatca gggaaactgt
      421 cccggtacag gctaacgata ggatcatctt ttggtatggt catcgcacgt gttgtttata
      481 gtaagtgtct aaaaaggtag gatgatcacc ccggatgcac aaaaaaatca atgcctttca
      541 agtagcgggg ttgccgctga cgtaaaacag ttggaccctg tgtcgatgga atactaactt
      601 aaagctgtca accaattgtc acacctcaac ttccggtaaa atgccaaagc taaccattgc
      661 caggtatgcc gtcatcccgc tactggcgct tgttgccctt tcttcctaca gtcagcccat
      721 ttcaactccc cggacaccca gccccgcagc gtcggtgtca cagaccgtcg ggatctccac
      781 catcaccgta aactattcca ggccatccgt caaaagcaga aaggtctggg gcgagttggt
      841 gccctatggc tggaacaaac agggattcgg cgcgggcaac gatgccccgt ggcgcgccgg
      901 cgccaacgaa aataccgttc tgcggctgtc gcatgatgcc acgatagaag ggcatccggt
      961 ccccgcaggc gagtacggcc tcttctttgt gatcaatcaa gacaataccg gggaagtggt
     1021 cctgtcgaaa gacagcaggt cctggggcag cttttggtac gacccgcagc atgacgagat
     1081 gagagcaaaa ataacgctgc acgccattcc gcagaccgaa atgctgacgt atgatttcca
     1141 gaacattacc cgcaattccg ccgagctggc gctgaattgg gaaaaggtgc agttccccgt
     1201 aaagatcgag ttcgccgtgg acgacatcgt gatcgccaat gcggccgaag agctcaaggg
     1261 ccccaccggc tttaactggc agggttacgc cagtgccgcc aattatgcgg ttcagaacaa
     1321 gatcggatac gataaggcgc tgctatggat cgatcaggcc atcgccatga attccaattt
     1381 taccacgttg aagatcaaat cggatctgct tcgggcaacc gggaaaacac ccgaagcgga
     1441 aagcatgctc aaagatgcta ctgccgttgc gacggaagtc gagctcaacc aatacgggta
     1501 tcagctgctc ggcaacaacc agcaagataa agcaattgaa atattcgtgc tcaataccca
     1561 gcgtcatccc aagagcgcca acgtcttcga cagtttgggg gaagcctatg cgctaaaggg
     1621 tgataagaag aatgccgtga tcaattttaa gcgatcgctg agcctgaatc cgccggccaa
     1681 tgtaagggcg aattccgaga aatatctcaa acagctgggc gaaatgtagg ggtaagttca
     1741 cctaagttgt tagtcccgag acggtaaaaa ggatcagcag caggccgatg atcatcattc
     1801 cccaatgcac gtaggtgaaa aaggaggagc cctgcagcct gcgattgaaa aaccttccca
     1861 ggaagatggc agggaggacc gccggcaggc agaccaggaa ataggtgaat aattcccgcc
     1921 ccattaaccc tttcagggcg taacccgcga tcccgaagag gctggcgggc agaaaatagg
     1981 cctggagcgt ggccctgaaa tgggtggggc tccatttccg catgttccca tagaccacca
     2041 gcggagggcc gttgagtccg tatgccccgc cgaatacacc agataaaaac ccgcagataa
     2101 aaagccatgg acggctgtcc tgcgcaaggg ccaggctggc cttccccagt atcgtgtaga
     2161 agctgtaacc gatgatcagc acccccaatc cgatcttcac ccagtaggca ttcccataga
     2221 tcaggatcat gaggccgatg ggaattccca aaagagcgta caggatcagc cacttggcgc
     2281 tgctgacgtg gatctgccgg tgatcctgga ccacgacgat caaggcgaca agaacggaga
     2341 taaggacgga aagcgggaca gcgacaggca acggaataaa aagagcgaaa agaggaacgg
     2401 cgaccaggga ttccccgaaa ccgaatgtcg accgtaccaa cgttgcaaaa aagcagatga
     2461 taattaccgg caagagcatt ttttgaatct gaattattta acctattttc aatttacaac
     2521 aacctttatg aactgggacc ccaaatatgc caaaatcgg
//
LOCUS       DATGKE010000050         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_192707, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000050 DATGKE010000000
VERSION     DATGKE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192707"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VKU83_00830"
     CDS             complement(<1..466)
                     /locus_tag="VKU83_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLI92115.1"
                     /translation="MKKAFLQLHIAVLLAGLTGVLGKLISLNAGVLVWYRLFITAVSL
                     WLIAVLRRQDILISRRDLLRISAIGGIAALHWVAFYASIKYSNVSVGLLCFSAVGFFT
                     AIIEPLVMRHRHDVVELFLGLLVIVGIFFIFQLDPRYKTGIVIGLISSLLASV"
     gene            complement(497..1288)
                     /locus_tag="VKU83_00835"
     CDS             complement(497..1288)
                     /locus_tag="VKU83_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HLI92116.1"
                     /translation="MEKIVIIPTYNEKENISNILDAIFQLGQGYHVLVIDDQSPDGTA
                     GIVRTLQPKYLGQLFLEERHGKLGLGTAYIHGFKWAVRNNYQFIFEMDADFSHNPRDL
                     DRLYEACKHQGGDVAVGSRYVKGGGNVNWPKDRILLSRGGSIYTRLITWMPVQDPTAG
                     FVCYRKEVLETINLDQIRFVGYAFQIEMKFAAWKLGFKIKEVPIIFEDRKFGVSKMNK
                     GIVKEGILGVLNLRWQSLFKNYRNRVKNDPVTMRPFSQEATATSK"
     gene            complement(1377..2144)
                     /locus_tag="VKU83_00840"
     CDS             complement(1377..2144)
                     /locus_tag="VKU83_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLI92117.1"
                     /translation="MLQLTRPLAIIDLETTGINLGTDRIVEIAILKIQPDGKKLVKRK
                     LLNPEIPISAISTEIHGITNEMVKDAPTFRQVANELKQFLDHCDLAGYNSNRFDIPML
                     AEEFLRVGMEFEGKGRKLLDVQKIFHMMEQRTLTAAYRFYCNKELEGAHGAEADATAT
                     WDVLVAQVLRYPQLGTNIDTILKHIGEEPLVDFARRFVLENGVEVFNFGKYKGRPVAD
                     VLKAEPQYYDWMMKGDFPLHTKQKLTEIFNRTMLKKG"
BASE COUNT          576 a          607 c          510 g          559 t
ORIGIN      
        1 agacgctggc cagcaaggac gatatcaatc cgatcacaat gccggtctta taacgtggat
       61 caagctggaa aataaaaaag atgccaacga tgacgagcag ccccaaaaag agttccacga
      121 catcatgcct gtgacgcatg accagaggct caatgatggc ggtaaagaaa ccgacggctg
      181 aaaaacacaa aagaccgaca ctcacattgg agtacttaat actcgcgtag aaggccaccc
      241 aatgcaaggc ggcaatgccc ccgatcgcgg aaatccgcaa aagatcccgg cggctgatca
      301 ggatatcctg ccggcgcaac acggcgatca accaaagcga aaccgccgta atgaacaacc
      361 tgtaccaaac caacacgcct gcattcaagg atataagctt cccgaggacc ccggtaagac
      421 ccgcgagaag aacagcgata tgcagctgga gaaatgcctt cttcatgtag ccatggcaaa
      481 tgggatttgc ctcaaattat ttagaggttg ctgtcgcttc ctgagaaaat gggcgcatgg
      541 ttacggggtc attcttaacc cggttgcggt agtttttgaa cagggattgc cagcgtagat
      601 tcagtacacc gagtatacct tcctttacga ttcccttgtt cattttggaa acgccaaatt
      661 ttctgtcttc gaaaatgatc gggacttcct tgatcttgaa tcccagcttc caggcggcaa
      721 atttcatctc gatctgaaaa gcataaccga caaaacggat ctggtcaagg ttaattgtct
      781 cgagcacttc ctttcgatag cagacgaagc ctgccgtcgg gtcctgaacc ggcatccacg
      841 tgatcagccg ggtataaatg gagccccccc gcgacaggag gatcctgtcc ttcggccagt
      901 tgacatttcc cccgcccttc acatatctgg aaccgactgc cacgtctccg ccctggtgtt
      961 tgcaggcctc atacaatcga tccaggtccc tggggttgtg ggaaaaatct gcatccatct
     1021 caaaaataaa ctggtaattg ttccgcactg cccatttgaa cccgtgaata taggctgtgc
     1081 cgagacccag tttaccatgc cgctcctcca aaaaaagctg cccgagatat tttggctgaa
     1141 gcgtccttac tatacctgcg gttccatccg gtgactggtc atcaattacc aaaacatggt
     1201 acccttgtcc caattgaaag atagcgtcca ggatatttga aatattttcc ttttcgttat
     1261 acgttggtat gatgacgatc ttttccaaat ggctggtgta ctaacggatg aaaagccctt
     1321 tcagggctgg ctcataagcg caaaagtaca acttttagcc gcccgcgcct gctttctcat
     1381 ccctttttta acatagtcct gttaaatatc tcggtcagtt tttgtttcgt gtgcaacgga
     1441 aaatcaccct tcatcatcca atcgtaatac tggggctccg ctttaagcac atccgccacc
     1501 ggccggccct tgtactttcc gaaattgaac acctcaacgc cgttttcgag cacaaaccgg
     1561 cgggcgaagt ctacgagtgg ctcctcccct atatgtttga ggatcgtatc gatattggtg
     1621 cccaattggg ggtacctgag aacctgcgcc acgagcacat cccaggtggc cgtcgcatcc
     1681 gcttccgccc cgtgtgcacc ttccagctcc ttgttgcagt aaaacctgta ggctgcggta
     1741 agcgtgcgct gctccatcat atggaagatt ttctgcacat ccaggagctt gcgacccttc
     1801 ccttcgaact ccatccctac ccgaagaaac tcttccgcca gcatcgggat gtcaaaacgg
     1861 ttggaattat agccggcaag atcgcagtga tccaaaaatt gtttcaattc attggcaacc
     1921 tggcggaatg tcggcgcatc tttcaccatt tcattcgtga taccatggat ttccgtcgat
     1981 atagcggata tcgggatttc cggattcaac agtttgcgtt tgaccagctt tttcccatcg
     2041 ggctggatct tcagaatggc gatctcgacg atacggtccg tccccaaatt gatcccggtc
     2101 gtttcgagat caatgatcgc caatggtcgt gtaagttgta gcatggtgca gatattataa
     2161 aaactcttac tgtatgatct cggcggatac cgcctggata tccagaccat cgtaattacc
     2221 cgaactcatt aaaagcaaat taacgttttt gt
//
LOCUS       DATGKE010000051         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1226442, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000051 DATGKE010000000
VERSION     DATGKE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226442"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VKU83_00845"
     CDS             <1..335
                     /locus_tag="VKU83_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92118.1"
                     /translation="VGNVSFYDPQQENSFTKPYSEETSKLIDEEVRKLIDKAYEATKH
                     LLTIKHREVEMLAEALLQKEVLFQSDVEALIGKRPYEEKRALDVTDHESNGVPPAVVV
                     PPATEEVK"
     gene            345..989
                     /locus_tag="VKU83_00850"
     CDS             345..989
                     /locus_tag="VKU83_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLI92119.1"
                     /translation="MNVSSGKENILKKIRKALSDSTPIPFPQSEGNHSVFQPSREDLE
                     IEFAEQFTKLLGKFLFCIDHHELAGQLKTLAGHQGWKKIVCREEKLQSLLRENGFADF
                     GDSGLSECDAAITSCESLVARTGSIVMSTGGGNASGRTVSVYAPVHICIAWSDQLVYD
                     IRDSLVALKEKYQQQMPSLITLATGPSRTADIEKTLVVGVHGPREVYLFLVDKR"
     gene            1036..1824
                     /locus_tag="VKU83_00855"
     CDS             1036..1824
                     /locus_tag="VKU83_00855"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLI92120.1"
                     /translation="MQIPAGKKIYFLSDFHLGAPDRASSLGRERRIVAFLERARQDAA
                     TIFIVGDLFDFWYEYRTVVPKGYTRILGKLAEITDGGVPIHFFTGNHDMWMKGYFEQE
                     LQIPVYHEPKGFEYNGKKFYIGHGDGLGPGDHGYKTLKKIFRNRFCQWLFGILPPAAG
                     VGLASWLSRRSRAVTGQIDERFLGEDGEWLIVYCKEVLQREYFDFMIFGHRHLPIDYR
                     LGNSGSRYINLGDWIRYNTYAVFEGERLALLADSPETAGKIVRH"
     gene            1821..>2132
                     /locus_tag="VKU83_00860"
     CDS             1821..>2132
                     /locus_tag="VKU83_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92121.1"
                     /translation="MKYGSIILLTGLLAATGLRVQGQEEPPPGSASGFALTLQLPQNI
                     VDFNVDNLGNIYVINRDNQLKKLSPRGDSLAVFNDVIRYGKVASIDVTNPLKILVYYR
                     DF"
BASE COUNT          482 a          576 c          628 g          446 t
ORIGIN      
        1 aggtgggaaa tgtcagcttc tacgatccgc aacaggagaa ttcattcacc aagccctatt
       61 ccgaggagac atcgaagctg attgacgagg aagtgcgcaa actgatcgac aaagcttacg
      121 aagcgacgaa acatcttctg accatcaaac accgggaagt ggagatgctg gcggaggctt
      181 tgctgcagaa agaggtgctt ttccagagtg acgtggaagc gctgatcggc aaacggccct
      241 acgaagaaaa gcgggcgctg gatgtcaccg atcacgagag caatggggtg ccgcccgccg
      301 tcgtggtgcc gcccgccacc gaagaagtaa aataaatacc acggatgaat gtatcttccg
      361 gtaaagagaa tattttaaag aagatcagga aggcgttgag tgattcaacg cctattcctt
      421 ttccccaaag cgagggaaac cattctgtgt tccaaccttc ccgcgaggac cttgagatcg
      481 agttcgccga acagttcacg aaactcctgg gcaagttcct gttctgcatc gatcatcatg
      541 agctggccgg gcagctgaaa acgctggccg gccaccaggg ctggaaaaag atcgtctgcc
      601 gggaggagaa gctacagtcg ctgctgcggg aaaatggatt cgccgatttc ggcgattcgg
      661 gcctgagcga atgcgatgcc gcaatcacca gttgtgaatc cctcgtggcg cggaccggta
      721 gcatcgtgat gagtacgggc ggcggcaatg caagcgggcg gacggtcagc gtgtatgcgc
      781 cggtgcatat ttgcatcgcg tggtcggacc agttggtcta cgacatcagg gacagcctgg
      841 tcgcgctgaa ggaaaagtac cagcagcaga tgccttcgct gatcaccctt gccacggggc
      901 ccagccgcac ggcggatatc gagaagaccc ttgttgtcgg cgttcacggc cccagggagg
      961 tgtatctttt cctcgtcgac aagcgctagc tggggtaacg ccggacaaaa tttttgtgga
     1021 attcaatatt cgcgcatgca gatccctgcc ggaaagaaga tctatttcct ctcggacttt
     1081 cacctcggag cgcctgaccg cgcgagcagc ctcggccggg agcggcggat cgtagccttc
     1141 ctggagcgcg cccggcagga cgcggccact atttttatcg tgggggatct ctttgacttc
     1201 tggtatgagt accggacggt tgtccccaag ggttataccc gcatactcgg aaaactcgcg
     1261 gagatcaccg acgggggcgt ccccattcat ttctttaccg gcaaccacga catgtggatg
     1321 aagggttatt tcgagcagga actgcagatc ccggtctatc atgagccgaa ggggttcgag
     1381 tacaatggaa agaagttcta tatcggccac ggcgatggcc ttggccctgg cgaccacggc
     1441 tataaaacac tcaaaaagat atttcggaac cggttttgcc agtggttgtt cgggatcctg
     1501 cctccggcgg ccggagtcgg actcgcatcc tggcttagcc gccgcagccg ggcggtcacc
     1561 gggcagatcg acgagcgttt cctcggcgag gacggggaat ggctgatcgt ctattgcaag
     1621 gaagtcctgc agcgcgagta tttcgacttc atgatcttcg ggcaccgcca tctgccgatc
     1681 gactaccgcc tgggaaattc cggtagccgg tatatcaatc tgggggactg gattcgttat
     1741 aatacctatg cggtcttcga gggcgagcgg cttgctctcc ttgccgacag tccggagacc
     1801 gccggaaaaa ttgtccgcca ttgaaatacg gctccatcat actgcttacg ggattgctgg
     1861 ccgcgacagg gctccgggta cagggccagg aggagccgcc gcccggttcc gcttcgggat
     1921 tcgcgctgac gctgcagctg ccgcagaata tcgtcgactt caacgtcgac aatcttggca
     1981 atatttacgt cattaaccgg gacaaccagt tgaaaaaact cagcccgcgc ggtgattcgc
     2041 tggcggtatt caacgacgtc atccgttatg gcaaggttgc ctcgatcgat gttaccaacc
     2101 cgttgaaaat actcgtttac taccgcgact tc
//
LOCUS       DATGKE010000052         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_497933, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000052 DATGKE010000000
VERSION     DATGKE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497933"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3085
                     /locus_tag="VKU83_00865"
     CDS             <1..3085
                     /locus_tag="VKU83_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HLI92122.1"
                     /translation="ANDGRLELSPRGPKSEKNMPIDVFFTSLSEVHQSHAIGVVLSGT
                     ATDGTLGLKAIKEHGGFTFAQEQQSAAFDSMPQSAIDAEVVDFILVPEEIPLQVSKLL
                     KTLRNGLAEDKEGTKPAKEEAFRQLIALLRIRKGTDFTYYKQTTIRRRVIRRMGLNKK
                     EDIADYLAYCRENLAEQDLLYQDLLIPVTGFFRDPRAFQVLCDSVFPALLKGKDEFNP
                     LRIWIAGCSTGEEPYSMAICLDEYLGGKAADYKIQIFATDISESSVAKARSGIYTKRD
                     IGGLSADRIERYFTKVNGSFHVNKSLRDMCVFACHNFLKDPPFAKMSLISCRNVLIYM
                     ETFLQKKALTTFHYALNENGYLLLGHSETSAPAADLFIPSGKGEKLYLRKNVSGKFLH
                     LSSDRNESKSKDNAYAVKKEPGRDDFQWTADQIVIERYSPPGVVVNEEMDIVQIRGLT
                     GDWLEPSPGKPSLNVLKMVRQGLAFELRNALHKAKSDNKPAVKQGIPLLSREKQQLVT
                     IEVIPLLNTVERYYLVLFRDTTAADATDTKPGGTTNESGGHDTPESRRIGQLEKELVQ
                     LREDMRSITEDQEAVNEELQSANEELLSGSEELQSLNEELETSKEEIQSTNEELTTLN
                     QELFDRNGQLNAARLYSESIIATIREPLIILDKDMKVKTANRAYYRKFMTSEELTEDK
                     YFYHLGNGQWDIPSLRSALENSLEAEGRVSDIEIRHRFEDIGERTLVLNACRIAAKES
                     AELLILVAIEDVTDARNREADLVSFSRALEDKVAERTNALKESNASLKRSNDSLEQFA
                     TIASHDLQEPLRKIQTFSALLLQRHSKNTGGEAGQLIGKIGVASERMSALIRDVLHFS
                     KVLDASVFERVDLNEIMQNVIGDFDMQIEQKAAIITHDTLPVIQAVRLQMNQLFYNLF
                     SNALKFSKPDVPPVIHISSKVFAAGETPGNIVVNGNTAYCEIIFHDNGIGIDKKFLEQ
                     VFLIFRRLNARERFEGTGIGLALCKKIVLSHRGEIYVTSGEAGGTQFHIVLPFAQ"
     gene            3259..>3841
                     /locus_tag="VKU83_00870"
     CDS             3259..>3841
                     /locus_tag="VKU83_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLI92123.1"
                     /translation="MTTSLQSMASEPVVRNNGCPDKSMTEIIPNGFFIVNHDWTVKHW
                     NKAAEVLLTVKAEDIVGRNLWEAFSGVIPLQFYAVYHKAFLRDIPVHFVEYWEEMGAW
                     FDVVTYYYEDSLSVSFKYSNQPAHHELLEERLRTLNELYRYVTEVTNDCLWEWDLTAE
                     ELYWIDGGHKRVFGYPIVNAIIPQQFWENRLHPD"
BASE COUNT         1019 a          848 c         1068 g          906 t
ORIGIN      
        1 ggccaacgat ggacggcttg aattgagtcc ccgaggccct aagagcgaaa agaatatgcc
       61 catcgacgtg ttttttactt cgctatccga agtgcaccag agccacgcga ttggggtggt
      121 actgtcggga acggcgacgg acgggacgct ggggctcaag gcaatcaagg agcatggtgg
      181 atttaccttc gcccaggagc agcagtcggc tgcttttgac agcatgccgc aaagcgccat
      241 tgacgcggag gtggtggact ttatcctggt gccggaagag atccctttgc aggtctcgaa
      301 gctgttgaaa acattaagaa atggcctggc tgaggataag gagggcacaa aaccggcaaa
      361 agaagaggcg ttccggcaac tgatcgcctt gttgcggatc aggaagggga cggattttac
      421 gtattataag cagacgacca tccgaaggcg ggtcatccgc cggatgggac tgaataaaaa
      481 ggaagacatt gcggattacc tggcttactg cagagagaat ctggcggagc aggacttact
      541 ttaccaggac ctgctgatcc cggtgacagg gtttttccgc gatcccaggg cctttcaagt
      601 cctatgcgac tccgtgtttc cagcgctatt aaagggtaag gacgagttca atccgttgcg
      661 gatatggatc gccggttgtt cgacggggga ggagccttat tcgatggcca tatgcctgga
      721 cgaatattta gggggaaagg cagccgacta taagatccag atctttgcga cggatatttc
      781 ggagagctcg gtggcaaagg ccaggagcgg aatctatacc aaaagggata tagggggatt
      841 aagcgccgat cgaatagaaa ggtattttac aaaagtaaat ggcagctttc atgttaataa
      901 gtctctcagg gatatgtgtg tctttgcgtg ccacaacttc ctgaaggatc cgccttttgc
      961 gaaaatgagc cttatcagtt gccgcaatgt cctgatctat atggaaacct tcttgcaaaa
     1021 gaaggcattg acgacctttc attatgcgct gaatgaaaat gggtacttgt tgctgggtca
     1081 ttctgaaacg tcggctcctg cggcggatct gttcattcct tccgggaaag gcgaaaaatt
     1141 atatctcaga aaaaacgtgt ccggaaagtt cttgcacctt tcgtcggaca ggaacgaaag
     1201 taaatcgaaa gataatgcct atgcggttaa gaaggaaccg gggcgggacg attttcaatg
     1261 gaccgcggat cagatcgtca tagagaggta ttctccccct ggcgtggtgg tgaacgaaga
     1321 aatggacatc gtgcagatcc gggggctgac aggtgactgg ttggaaccat caccggggaa
     1381 gccgagcctg aatgttttga aaatggtgag gcaggggctg gcttttgagc tccgaaatgc
     1441 ccttcataag gcaaagtcgg ataataaacc ggccgtcaaa caaggtattc ccctgctttc
     1501 ccgggagaaa caacagctgg tgacgatcga agtgattcct ttgttgaata ccgtggagcg
     1561 ctattatctg gtccttttcc gggatactac ggctgccgat gcgacggaca caaagcccgg
     1621 cggaacgacc aatgaatcag gcggccatga taccccggag agccgccgta tcgggcaatt
     1681 ggaaaaggaa ctggtgcagc tgcgggaaga tatgagaagt atcacggaag atcaggaagc
     1741 agttaatgag gagttgcaaa gcgccaacga ggaactgctg tcgggcagtg aagaactgca
     1801 aagcctgaac gaggagcttg aaacgtcgaa agaagagatc caaagcacca atgaagagct
     1861 cacgaccttg aaccaggagc ttttcgaccg gaatggtcaa ttgaatgcgg cccggctgta
     1921 ttcggaatct attatcgcca cgatccggga accgctcatt atcctggata aggacatgaa
     1981 ggtaaagacg gccaaccggg cttattaccg gaaattcatg acgtcggaag agctgacaga
     2041 ggataaatat ttttatcacc ttgggaacgg ccaatgggat attccatcgc ttcggtcggc
     2101 cctggaaaat agtctggagg cggaaggccg ggtttccgat atagagattc gacacaggtt
     2161 tgaagatatt ggcgaaagaa ccctcgtgct gaatgcctgc cggatcgccg caaaagaaag
     2221 tgccgaatta ttgatcttag tggccataga ggatgttacc gatgcgagga acagggaagc
     2281 tgacctggtt tccttttccc gggcactgga agataaagtg gccgagcgga cgaatgccct
     2341 taaagagtcc aatgcctcgc tcaaacgatc gaatgatagc ctggaacaat ttgcaacgat
     2401 cgcttcccac gatttgcagg aacccctgcg aaaaatacag accttttcgg ccctcttgct
     2461 gcaacggcac agtaaaaata caggcgggga agcagggcaa ttgattggga agattggcgt
     2521 ggcctcggag cggatgtccg cgttgatccg tgacgtgctt catttctcaa aagttttgga
     2581 cgccagcgtc tttgaacggg tggacctgaa cgaaattatg caaaatgtca tcggtgattt
     2641 tgatatgcag atcgagcaga aagctgcgat cataacgcac gatacacttc cggtcattca
     2701 ggcggtgcgt ttgcagatga accagctttt ttacaattta ttcagcaacg ccctcaaatt
     2761 ctccaagccg gacgtgccgc cggtgatcca catttcctcc aaggttttcg ctgccggaga
     2821 aacgcccggt aatattgtcg tcaatggcaa tactgcttat tgtgaaatta tctttcacga
     2881 taatggtatc ggaatcgata aaaagtttct cgagcaggtt ttcctgatat tccggcggct
     2941 gaatgcgcgc gagcgttttg aaggtacggg tatcgggctc gcgttatgta aaaaaatcgt
     3001 attaagtcat cggggggaaa tttatgtgac ctccggtgaa gccggaggaa cccagttcca
     3061 tatcgtcctt ccctttgctc aataatgcgg agaacgtgtg aatcatggaa caaattcccg
     3121 gaatcgtgaa ccatcttcct tattgaattg aggtaacttt ccaagttcgc cgggcatcaa
     3181 caaaatggcc tgttggaacg atgtaagcgt ctccaatggc cggccgttga ttgccctggc
     3241 caattaataa aaatcgatat gacgacttca cttcaatcaa tggcaagcga accggtggtc
     3301 aggaataacg gctgcccgga caaatcgatg actgaaataa ttccaaacgg cttctttata
     3361 gtgaatcatg actggactgt gaagcattgg aataaggcag cggaggtttt gttgaccgtc
     3421 aaggcggaag acatcgtcgg gcgtaacctt tgggaagcct tctccggcgt tatcccgttg
     3481 caattctatg ccgtctacca caaggcattc cttcgggata ttcccgttca ttttgtagaa
     3541 tattgggagg agatgggggc ctggttcgat gtcgtcacct actattacga agattcgttg
     3601 tccgtttcct ttaagtatag taatcagccg gctcatcacg aacttcttga ggagcgattg
     3661 aggaccttga atgagctgta tcgatatgtt accgaggtta ccaacgattg tctttgggaa
     3721 tgggacctta ccgcagagga gttgtattgg atagatggag gccacaaaag agtttttggc
     3781 tatccaattg taaatgcaat tattcctcaa cagttttggg aaaatcgtct gcacccggac
     3841 g
//
LOCUS       DATGKE010000053         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1437130, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000053 DATGKE010000000
VERSION     DATGKE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437130"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VKU83_00875"
     CDS             complement(<1..380)
                     /locus_tag="VKU83_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92124.1"
                     /translation="MRKMRKWWWLLIPLLLIIFFLAGPKPADPVYSTMMPVVPADPSG
                     LTDYIRTMEATHRLKPDNEARIVWADSSHRKTRYAIVYLHGFSASQAEGEPTHRYIAE
                     RYGCNLYLSRLADHGVDTPEAMVNL"
     gene            525..>2034
                     /gene="typA"
                     /locus_tag="VKU83_00880"
     CDS             525..>2034
                     /gene="typA"
                     /locus_tag="VKU83_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HLI92125.1"
                     /translation="MQIRNIAIIAHVDHGKTTLVDKILHATHVFRENQETGELIMDNN
                     DLEKERGITIFSKNAAVEYKDTKINVIDTPGHSDFGGEVERVLKMADGVLLLVDAFEG
                     PMPQTRFVLQKALQLNLKPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLDFPT
                     FYGSGKNGWFNSKNEPCADITPLLDGILKYVPAPKVSEGPLQMQITSLDYSSFLGPIA
                     IGKVARGVIKEGQPIALMQADGVIKRSKVKELYTFMGMGKKKVAEVAAGDICAVVGLE
                     DFNIGDTIADAENPEALPVISVDEPTMSMLFGINNSPFFGKDGKFVTSRHLRDRLMKE
                     TEKNLALKVHDTDSADSFLVYGRGILHLGILIETMRREGFELTVGQPQVLVKEIDGKK
                     NEPYEILVVDVPAEFSGKVIDMVTQRKGEMHVMESKGEMQHLEFEIPSRGLIGLRSNM
                     LTNTAGEAVMAHRFLEYKPWKGPIPGRNNGVLLSKNQDKTTAYSIDKLQERGT"
BASE COUNT          514 a          542 c          568 g          410 t
ORIGIN      
        1 aggttgacca tggcctccgg cgtgtcgacg ccgtgatcgg ctaaccggga aagatagagg
       61 ttgcaaccat atcgttcggc gatataccga tgcgttggct ctccttccgc ctgcgaagcg
      121 gagaagccgt gcagataaac gatagcgtaa cgcgttttcc tatgggaaga atcggcccat
      181 acgatgcggg cttcattgtc cggcttcaac cggtgcgtcg cctccatcgt gcgtatataa
      241 tcggtcagac cggatggatc tgccgggacg acgggcatca ttgtggaata gaccggatcg
      301 gcaggctttg gcccggctag gaaaaagatg atcaggagca gggggatcag cagccaccac
      361 catttacgca ttttccgcat gggatgaaaa tttttgggaa atataaggta tctgacatgt
      421 tttttgcgtt atttctactt aaacgctccg gatcaactag gaaatccggg tgcttttgcg
      481 taactttgcg cgcccaaaag ggggccacaa ggaaagagaa atttatgcaa atcaggaaca
      541 tagcaatcat tgcccacgtc gatcacggta agactaccct ggtagacaag atcttacatg
      601 ccacccacgt attccgtgag aaccaggaga ccggcgaatt gatcatggac aacaacgacc
      661 tcgaaaaaga acgggggata accattttca gcaaaaacgc tgcggtagag tataaagaca
      721 cgaaaatcaa tgtaatagac accccgggtc actccgactt cggaggcgag gtggaacggg
      781 tattaaagat ggccgatggc gtgttgctgc tggtggatgc tttcgagggc ccgatgccgc
      841 agacccgttt cgtgctgcag aaagccttac agctcaacct gaaacccatc gtggtcatca
      901 ataaggtaga caagccgaac tgccgtcccg acgaagtgca tgacgcagtg ttcgagctct
      961 tcttcaacct ggatgccacc gaagagcagc tggacttccc caccttctat ggtagcggca
     1021 agaacggctg gttcaacagc aagaacgagc cctgcgcaga tataaccccg ctcctggacg
     1081 gcattctgaa atatgtgccg gcccccaagg tgtccgaagg ccccctgcaa atgcagatca
     1141 cttcgctgga ctattcttcc ttcttagggc cgatcgctat aggaaaggtt gcgcgcggtg
     1201 tcattaaaga aggccagccc atcgcgctca tgcaggcgga tggcgtcatc aaacgttcca
     1261 aagtgaagga gctctatacg ttcatgggta tgggcaagaa gaaggtggcc gaagtagccg
     1321 ccggtgatat ttgcgccgtc gttggtcttg aggatttcaa catcggggac accatcgccg
     1381 atgccgagaa ccccgaagcc ctgccggtga tcagcgtcga cgagcctacc atgagcatgc
     1441 tcttcggcat caataactcg cctttctttg gaaaggacgg aaagttcgtg acctcgcgcc
     1501 atctgcgcga ccggctgatg aaggagaccg aaaagaacct tgcccttaag gtacatgata
     1561 cggacagcgc cgatagcttc ctggtttacg gtcgcggtat cctgcatctg ggcattctga
     1621 tcgagaccat gcgccgtgaa gggttcgagc tcaccgtggg tcagccccag gtgctggtaa
     1681 aagagatcga cggcaagaaa aatgagccct atgagatcct ggtcgtcgac gtccccgccg
     1741 agttcagcgg aaaggtcatc gatatggtga cgcaacgtaa gggcgagatg cacgtgatgg
     1801 aaagcaaggg cgaaatgcag cacctcgaat tcgagatccc ttcccgcggc ctgatcgggc
     1861 tgcgcagcaa tatgctcacc aatacggcag gcgaagccgt catggcacat cgcttcctgg
     1921 aatataaacc ctggaagggt cccatccccg gccgcaacaa cggtgtcctg ctctccaaga
     1981 accaggacaa gacgacggcc tactccatcg acaagctgca ggaacgcggc acct
//
LOCUS       DATGKE010000054         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1535943, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000054 DATGKE010000000
VERSION     DATGKE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535943"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..503
                     /locus_tag="VKU83_00885"
     CDS             132..503
                     /locus_tag="VKU83_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLI92126.1"
                     /translation="MKSLTKAEEQVMLVLWRLGKGFLKDIVDATPEPRPHPNTIATLL
                     KILVEKGFVEFDVQGRNNCYRPLVSKAAYAKTSANQLVKGYFEGSAAKLVSQFVTDKT
                     LSLQDLETLLQQIRAARKSKS"
     gene            500..2014
                     /locus_tag="VKU83_00890"
     CDS             500..2014
                     /locus_tag="VKU83_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56/M15 family metallopeptidase"
                     /protein_id="HLI92127.1"
                     /translation="MKEFLFTAAIPYLAKVLLASAFFYGYYRLFLRDKVFHQYNRFYL
                     LGASVGPLLAPLVRLRLPAAVSLIDRAPVLVLHAIAPGSWSEGEGIAGATNHSLTGHG
                     SGAAILIAVYGLTVLFALLAFLRQLWYIRRLNGRYPPEKLGPVKLYMTREPGTPFSFL
                     NRLFWNEDIDMDSERGRQIFLHEWWHIRQRHTLDLLWLRLTMVFFWINPFFYLIYREI
                     RTIHEYLADQFAVDGGDRYDYAELLVWRTVSNRSLSLLHPFFQSSIKRRITMLTRFHA
                     SRPGYISRMMVLPLTFLLLCAFAVRKPSLTSPPRLFTVVIDAGHGGPDNGAVSGDVKE
                     KDLDLALALKVQQLAPKYHIHVVLTRKRDELAGGKSSIHSSLEYRAAMARDVKADLFV
                     SLHVNNSGGGTPTHGFVAFVSRDNAHYSQSTQLASALLDAVKRTYATDENIRQREEGI
                     YVLREAQMPAVLLECGSIDDPADLAFIRDGQQQEKVARDILAGIQRYQEAQVGR"
     gene            2151..>2350
                     /locus_tag="VKU83_00895"
     CDS             2151..>2350
                     /locus_tag="VKU83_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92128.1"
                     /translation="MNHPTGHQATDRQLVELVLSGDRQAFNRIILATEGLVAQIVFRL
                     IPNEEDRKDVAQDIYLKVFHHL"
BASE COUNT          532 a          643 c          641 g          534 t
ORIGIN      
        1 aggtaacaaa aaagtgaatt ttgatgcccc cgggcggggg gtaaaaggtt ttcaaagaaa
       61 attatcgaaa taaacttgga gtaactaaaa atttagttat acatttacat aactaaaaac
      121 ttagttatta gatgaagtca ctcacaaagg cggaggagca ggtcatgttg gtcctttgga
      181 ggcttggaaa gggcttcctg aaggacattg tagatgcgac tcccgagccg agaccgcatc
      241 ccaatacgat cgcaaccctg ttgaagatcc tggtggaaaa aggatttgtg gaattcgacg
      301 ttcagggacg caataactgt tatcggccgc tggtaagcaa agcggcctat gccaagacca
      361 gcgccaatca gctggtgaag gggtatttcg aggggtcggc ggccaaactc gtctcgcaat
      421 tcgtaacgga taaaacatta agcttgcaag acctggaaac gcttttgcaa cagatccggg
      481 ctgcaagaaa atccaaatca tgaaagaatt cctgtttacc gccgctattc cctacctggc
      541 caaggtgttg ctggcctctg cgtttttcta tggctattac cgcctgtttc tgcgggacaa
      601 ggtttttcat cagtataacc ggttttacct gctcggcgcc agcgtgggtc cgctgttggc
      661 gccgctggtc cggctacgtc ttcccgccgc ggtatcgctg atcgaccgtg cgcccgtcct
      721 ggtcctccat gccatcgccc ccgggagttg gagcgagggc gaaggaattg cgggggcgac
      781 gaaccattca ttaaccgggc acgggtccgg agcagccatt ctcatagctg tgtatgggct
      841 gacggtttta tttgcattgc tggcatttct gcgtcagcta tggtacatcc ggcgactgaa
      901 cgggcgatat ccgccggaga agctgggacc ggttaaactt tacatgacgc gcgaacccgg
      961 cacgccgttc tcttttctta accggctatt ttggaatgaa gacatcgata tggacagcga
     1021 gcgcgggcga cagatctttc tgcacgagtg gtggcatatc cgtcagcgcc atacgctgga
     1081 ccttctctgg ctacggctga cgatggtctt cttctggatc aacccgttct tttacctgat
     1141 ctaccgggag atccgaacga tccatgaata tcttgccgac cagtttgcgg ttgacggcgg
     1201 tgaccggtat gattatgccg aactgctggt ttggcgcacc gtgagcaacc gctccctttc
     1261 cctcctgcat ccatttttcc aatcatccat aaaacgaaga attaccatgc tcacccgatt
     1321 tcatgcctcg cgacccggct atatcagccg gatgatggtc ctgccgctga ccttcctgct
     1381 gctctgcgct ttcgcggtca ggaaaccttc cttaaccagc cctccacgcc tttttacggt
     1441 ggtcatcgac gcgggccacg gcggccccga taacggtgcg gtctctggcg acgtcaaaga
     1501 aaaagacctc gatcttgctc ttgcgctaaa agtgcagcag cttgcgccga agtaccatat
     1561 ccatgtagtg ctcacccgaa agagggacga gttggctggc gggaaaagca gcatccatag
     1621 ttcgctcgaa taccgtgccg caatggccag ggatgtcaaa gcggatctgt tcgtctcgct
     1681 gcacgtcaac aattcaggcg ggggaacgcc aacacacggt ttcgttgcct ttgtttccag
     1741 ggacaatgcc cactattccc agtccaccca actggcatcg gcgttgctgg atgcggtaaa
     1801 gcgcacttac gcgacagatg aaaatatccg ccagcgcgag gagggtattt atgtgctgcg
     1861 ggaagcccag atgccggccg tcctgctgga gtgcggaagc atcgacgacc cggcggatct
     1921 cgcgttcatc cgcgacgggc aacagcagga aaaggtcgcc cgcgacatcc tcgccgggat
     1981 tcagcgctac caggaggcgc aggtagggag ataggttacc cctgccgtcc cgcagaccgg
     2041 accccttgcc gccagcaggc caggggttct ttttttatcc ccgtgaaaga aatacccctt
     2101 ttgtccggat ttattgcaac atttgagcga ccatccggtc taatcttccc gtgaaccatc
     2161 caacagggca ccaggcaacg gacaggcagc tcgtcgagct ggtattgagc ggcgacaggc
     2221 aagccttcaa ccgcattatc ctggcgaccg aggggctggt ggcgcagatc gtgttcaggc
     2281 ttatccccaa tgaggaagac cggaaggatg tcgcgcagga catctacctg aaagtattcc
     2341 atcatcttaa
//
LOCUS       DATGKE010000055         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1019389, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000055 DATGKE010000000
VERSION     DATGKE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019389"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VKU83_00900"
     CDS             complement(<1..418)
                     /locus_tag="VKU83_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HLI92129.1"
                     /translation="MMTVSHPWHGVPAGKGAPRVVNAIIEIPQGSRCKYEIDKDSGLL
                     KLDRVIYSSFYYPVNYGFIPQTYGDDKDPLDILVITSLPVQALTLMEAKVMGVMQMVD
                     SGDADDKIIAVAAHDPSVNHYNNIEELPKHFFEELRH"
     gene            complement(421..822)
                     /locus_tag="VKU83_00905"
     CDS             complement(421..822)
                     /locus_tag="VKU83_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92130.1"
                     /translation="MIRIFILPAVLLFAATACTHSFKKAEDAEDAGREFIRAALDGDY
                     DKANFYLYADSTNRMLLDKWKKDYDGLPSDEKQKYRDADIRPIDIHPVNDSVTSYTYA
                     NSYKKDTTTFRVVRINGEWLVDLKEFLNHKR"
     gene            complement(851..1855)
                     /locus_tag="VKU83_00910"
     CDS             complement(851..1855)
                     /locus_tag="VKU83_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLI92131.1"
                     /translation="MTETIINLETVNPIEFFGVNNGKLDILKKKFPLLKILSRGTQIK
                     LSGSPEQVESAKEKIDLLVQYLERNGHVSENYFEQILGGDDAETVDHFVDRHPNDILV
                     FGPNGKTVRARTVNQKRMVVAAEKNDIVFAIGPAGTGKTYTAVALAVRALKNKMVKKI
                     ILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMSTRTIEIAPL
                     AYMRGRTLDNAFIILDEAQNATDLQLKMFLTRIGANAKAIITGDLTQIDLPKNQKSGL
                     AKALYILQNIDGIAHIELDEEDVVRHRLVKAIIKAYERDKQEQEEQQERRAIKGKDQA
                     "
BASE COUNT          464 a          586 c          601 g          586 t
ORIGIN      
        1 aatgacgcag ctcttcgaaa aaatgtttgg gcagctcttc gatattgtta taatgattga
       61 cgctcgggtc atgcgcggca acggcaatga tcttgtcatc cgcgtccccg ctgtccacca
      121 tctgcatgac ccccatgacc ttggcttcca tcagggtaag cgcctggacg ggcagggaag
      181 tgatgacaag gatatcaagc gggtccttgt catccccata agtttggggg ataaaaccat
      241 agttgaccgg ataatagaac gaggaataga tcactcggtc gagcttcagc aagcctgagt
      301 ccttatcgat ctcatacttg cagcgcgagc cctggggaat ctcgatgatt gcgtttacca
      361 ctcgcggggc gcccttgcca gccgggaccc catgccaggg gtgggagact gtcatcataa
      421 ttatcgtttg tgattcagga attcttttag gtctacgagc cactcaccgt tgatccggac
      481 aacccggaag gtggtggtgt ccttcttgta tgaattggca taggtatacg aggtaacgga
      541 atcattgacc gggtggatat cgatcggccg gatgtcggca tcccggtatt tttgcttctc
      601 atccgaaggc aggccgtcat agtctttttt ccacttatcc aggagcatcc ggttggtgga
      661 atcggcgtac aggtaaaagt ttgccttatc gtagtccccg tcaagcgcgg cgcggatgaa
      721 ctcccggccg gcatcttcgg catcttcggc tttcttaaag gaatgggtgc aagcggtggc
      781 ggcaaaaagg agcactgccg gcagaatgaa tatcctgatc atccggtaaa ggtagtaaaa
      841 tgaccttaga tcaagcctga tccttcccct tgatcgcgcg gcgctcctgc tgctcttcct
      901 gttcctgctt gtcccgctcg taggccttga tgatcgcctt gaccagccgg tgccggacca
      961 cgtcttcctc atcgagctcg atatgggcta tgccatcgat gttctgcaag atgtaaagcg
     1021 ctttggccaa cccgcttttt tggttcttgg gaaggtcgat ctgggtcagg tcgccggtga
     1081 tgatcgcctt ggcattggcg ccaatacggg taaggaacat tttgagctgc aggtccgtgg
     1141 cgttctgcgc ttcgtccagg atgatgaatg cattatccag cgttcttccc cgcatatacg
     1201 ccagcggcgc gatctctatc gtgcgcgtgc tcatatagta gcccagcttg tcggccggaa
     1261 tcatatcatc cagcgcgtca taaagcggcc gcagataagg atcgatcttc tccttaaggt
     1321 ccccggggag gaacccaagg ctttcgccgg cctcaaccgc gggccgtgtc aggatgatct
     1381 tcttgaccat tttattcttc agcgcccgca cggcgagggc taccgcggta taggttttac
     1441 ccgtacctgc cgggccgatc gcaaacacga tatcgttctt ctcggcggcc accaccatcc
     1501 ttttctggtt gacagtcctg gcgcggaccg tcttgccgtt cggaccaaaa accaggatgt
     1561 cgttcggatg ccggtcgaca aaatgatcca ccgtttcggc gtcatcgccg ccaaggattt
     1621 gctcgaagta gttctcgctc acatgtccgt tccgctccag gtattgcacc agcagatcta
     1681 tcttttcttt ggcgctttcc acctgttccg gggagccgct cagcttgatc tgggtgccgc
     1741 gagataggat tttcagcaga gggaattttt tcttaaggat atccagcttt ccgttattta
     1801 caccaaagaa ttcaatgggg tttacggttt ccaggttgat gattgtctca gtcaaattca
     1861 atgcttttag cgttgagaaa tgggtactgc gcgggtgcca tagggccttt tcaaatataa
     1921 cccgtgcggt tcttattctt tcttaattct tattaacata tgtgaatacc aatggctttc
     1981 acgatcgaat aacaatacgc catacgactt tgttcgtaac cgcggcgaag catgccaatc
     2041 gcggatcctt gagttgacgg gagcaggcgt cgggatgaaa tggagcggaa atgatgaaga
     2101 cgccgtgttg gctcatgggt cagcgccttc ctgaaggagc atattcggtg cccttgccgg
     2161 gcattaaacc ttttatcggg agaacccgga ggctcccaat tcctcctcga tcaggttgtg
     2221 gaaggtggcc tcgtttt
//
LOCUS       DATGKE010000056         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1436864, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000056 DATGKE010000000
VERSION     DATGKE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436864"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1081
                     /gene="trpD"
                     /locus_tag="VKU83_00915"
     CDS             89..1081
                     /gene="trpD"
                     /locus_tag="VKU83_00915"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HLI92132.1"
                     /translation="MKKILQYLFEHKTLAREQARDVLTRISRSEFNESEIAAFITVFL
                     MRSITINELMGFQDALLELCVRVDLGGLETVDIVGTGGDGKNTFNISTLACFIVAGTG
                     NKVAKHGNYGASTISGASNVMEQLGYRFKNDAARLRQEIDVAGICFLHAPLFHPALKT
                     VGPIRKNLGIRTFFNMLGPMVNPAFPTTQLVGVYNLEMARIYNYLLQQTDRRFTIIHG
                     LDGYDEISLTGDTKVITNRGEKIMTPEELGRRMVNAADIYGGNTIEEAAKIFTRILKV
                     EGSWAQNAVVLANAAMALHATGVYAGYEEAYYAAVDSLESGRAFGALEKLISLQ"
     gene            1078..1884
                     /gene="trpC"
                     /locus_tag="VKU83_00920"
     CDS             1078..1884
                     /gene="trpC"
                     /locus_tag="VKU83_00920"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HLI92133.1"
                     /translation="MNILDKIIAHKRVEVAGRKTAVPVPVLEKSAFFYQPALSLKAAL
                     RDTGSTGIIAEFKRRSPSRGVINDKADVGQVTRGYVAAGAACLSVLTDNHFFGGSESD
                     LATARANEVPILRKEFIIDEYQILEARAIGADVVLLIAACLSPQEVRRLSVFARGLGL
                     EVLLEIHGERELDHICDETAIVGVNNRDLKTFTVDIDRSIRLSKRIPAGKLLVAESGI
                     SQVQTILQLREAGFRGFLIGESFMKQADPAAAFTGFVRELRENDRHSMEI"
     gene            1909..>2093
                     /locus_tag="VKU83_00925"
     CDS             1909..>2093
                     /locus_tag="VKU83_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92134.1"
                     /translation="MRIKVCGMTELDQVRQLDSMGVDFAGFIFYPKSPRYVVRHLSGE
                     QLKKARLRLGKVGVFVNP"
BASE COUNT          489 a          498 c          649 g          457 t
ORIGIN      
        1 gagggggtac aatttcaccc ggaaagtgtg ctgaccccgg acggtgagca gctgttgata
       61 aactggttaa aaaattaatg cgatccggat gaaaaagatc ctgcaatatt tgtttgaaca
      121 caagaccttg gcgcgcgaac aggccagaga tgtgttgacg cgtatctccc ggagcgagtt
      181 caatgaaagc gagattgcgg cgttcattac cgttttcctg atgcggagta taaccatcaa
      241 tgaactgatg gggttccagg atgccttgct ggaactttgt gtgcgcgtgg acctcggcgg
      301 ccttgaaacg gtcgatatcg tgggtaccgg cggagacggc aaaaacacct tcaacatttc
      361 cacgcttgcc tgtttcattg tggccgggac tggaaataag gtcgcaaaac acggcaacta
      421 tggcgcctcc accatcagcg gagcgtccaa tgtcatggaa caattaggct atagattcaa
      481 gaatgacgcc gcgcgcttgc gacaggagat tgacgttgcc ggcatctgtt tcctgcatgc
      541 gccgttgttt catccggcat tgaagacggt ggggccgatc cggaagaacc ttgggatccg
      601 aaccttcttc aatatgctcg gcccgatggt aaacccggca ttcccgacga cccaattggt
      661 gggagtgtac aatctcgaga tggcccgcat ctataattac ctgttgcagc agacagaccg
      721 tcggtttacg attattcacg ggttggatgg ctatgacgag atatccctga caggtgatac
      781 caaggtgatc accaaccggg gcgaaaagat catgacgccg gaggaactgg gccgaagaat
      841 ggtaaatgct gccgatattt acgggggtaa taccattgag gaggccgcga agatctttac
      901 gcgcatcctc aaggtagagg gaagctgggc acaaaacgct gtggtgctgg ccaacgcagc
      961 gatggcatta catgccaccg gcgtttacgc cggttatgaa gaagcctatt atgccgcggt
     1021 agacagcctg gaaagcggcc gggcatttgg ggccctggaa aaattaatct cgttgcaatg
     1081 aatatactgg ataaaatcat cgcgcataaa cgcgttgagg tggcggggcg aaagactgcg
     1141 gtaccggtac cggtgctgga gaaaagcgct tttttttacc agccggcctt gtcgctgaag
     1201 gctgccttgc gggacaccgg gtcgaccggg atcatcgcag agttcaagcg ccggtctccg
     1261 tcgcgggggg tgatcaatga caaagccgat gtcgggcaag tgacgcgggg ttatgtggcc
     1321 gccggggcag cctgcctttc cgttctcacc gacaaccact ttttcggtgg cagcgaatcc
     1381 gacctcgcaa cggcaagggc aaacgaggtc ccgatcctcc ggaaggaatt cattatcgac
     1441 gagtaccaga tccttgaagc gcgggccatt ggagcagatg tggtgctgct gatcgcggct
     1501 tgtctttccc cgcaggaagt ccggcggtta tcggtttttg cccgcgggct gggcctggag
     1561 gtgctgctgg agatccatgg cgaacgggag ttggatcata tttgcgatga gacggcgatc
     1621 gtgggcgtga acaaccggga tctcaagacc tttaccgtgg atatcgacag gtcgatccgg
     1681 ttaagcaagc ggataccggc gggaaagtta ctggtagcgg agagcgggat cagccaggtt
     1741 cagaccatcc tgcagttaag ggaggcaggt ttccgcggtt tccttatcgg ggaaagtttt
     1801 atgaaacagg cagaccccgc tgcagctttc accggttttg taagggagct gcgtgaaaat
     1861 gaccgccatt cgatggaaat ataggagggc tgttacggtt taaacgatat gagaattaaa
     1921 gtatgcggga tgacggagct cgaccaggtg aggcagctgg attcgatggg ggtggacttt
     1981 gcggggttta ttttttaccc gaagtccccg cggtacgtgg tgcgccacct gtctggcgag
     2041 caactgaaaa aggccagatt acggctaggg aaggtgggcg tcttcgtcaa ccc
//
LOCUS       DATGKE010000057         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_814749, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000057 DATGKE010000000
VERSION     DATGKE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814749"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VKU83_00930"
     CDS             complement(<1..923)
                     /locus_tag="VKU83_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HLI92135.1"
                     /translation="MTNKKFEPFVSAQTEMKEFTFRALLAGSIFGIIFGASTVYLALK
                     AGLTVSASIPIAVLAITLGRRLLKTTILENNIIQTTGSASESIAAGIVFTLPGFLFLS
                     TTDSGGFFNYAVIFIVAIFGGFLGTLLMIPLRRSLIVKEHETLPYPEGTACGSVLKAG
                     EKGGDFARAAFQGLGFAVVYALGQKLFRIIAETPLFDTVKSKGLSSIFPAAQISADIT
                     PEYMGVGYIVGPRIAGILVAGGVLSWLVFHPLLASLIDPATVAQQQVKLGLLADIHTA
                     GGAGGWDPVTKTFADPASAIYRAYVRLIGAGA"
     gene            complement(920..2599)
                     /locus_tag="VKU83_00935"
     CDS             complement(920..2599)
                     /locus_tag="VKU83_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HLI92136.1"
                     /translation="MSQATAHRARHPRGLYVLFMTEMWERFAYYLLAGILLIYLMDNK
                     TGGKGFSEKDASDLTGTFLALIWLPMFVGGMIADRYFGYIRSVFIGGVFLAAGYFGLI
                     IPGNTAMYISLGLVVIGNGFFKPNISTLLGNIYNKEELKPLKDNAYNIFYMGINIGSL
                     LCNFVAAYLRNKYGWGYAFGAAGAGMVIGLINLASNLRHVKEGDVRKQSLREDMTLGE
                     ISLFVFVPAITAAIIGYWLPGKLLHTTLMGSPSNDAFVFACIPILGFFLSLWVRARGE
                     DRKGIGALLFIFAISVIFWSLYYQNFTGYTLWTERHTDRSITTRWMEKGANFMDFLQT
                     VNTTPRKVDSMNAYMAPVRDDSGHIIKTMGPDLYFRNLPRDKWPPPGTGLKLANTEEF
                     QSIGPLFIVLLTPLLVGLLTWLRRRNREPTTPAKFGIALFLSGVSSLVMVFAVMSVPS
                     IYQYKVSPAWLWGTYFVITAAEIFLSPMGLSLVSKLAPARLTSLLMGGWFLTMSLGSK
                     VAGMMTSSWDKFPDKRIYFLIWFVAGVLGSALIFSRIKWLNRVVREKTGEK"
     gene            2700..>3231
                     /locus_tag="VKU83_00940"
     CDS             2700..>3231
                     /locus_tag="VKU83_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92137.1"
                     /translation="MKILLLGSGGREHALAWKLAQSPLCTSLHIAPGNPGTARCGTNV
                     DLSPLDFEGIRRHCTAAGIGMVVVGPEEPLVKGIVDFFLADPALRGIPVIGPSAAGAQ
                     LEGSKAFSKKFMDRHGIPTAAYREFNHTDFAAGIAYLQQHSLPVVLKADGLAAGKGVV
                     ICQNHVEAVAEFELMVQ"
BASE COUNT          733 a          904 c          955 g          639 t
ORIGIN      
        1 gcgccggcgc cgatcaggcg tacataggcg cgatagatgg ccgaggccgg gtcggcgaaa
       61 gtcttggtca ccgggtccca gccgccggcg ccgccggcgg tgtggatgtc cgccagcagg
      121 cccagcttga cctgctgttg cgcgacggtg gcgggatcta tgagcgaagc cagcagggga
      181 tgaaagacaa gccagctcag cacgccgccg gcaaccagaa tgccggcgat cctcgggcct
      241 acgatatagc cgacacccat gtattcgggg gtgatgtccg cagagatttg tgctgccggg
      301 aagatgctgc tcagtccttt gctttttacg gtatcgaaca acggcgtctc ggcgatgatc
      361 cggaaaagct tttgtcccaa cgcatagacc acggcgaagc ccagcccctg aaaagcggcc
      421 cgggcaaaat cgccaccctt ttcaccggct ttaagcacgg aaccgcatgc tgttccttcg
      481 gggtaaggca gcgtctcgtg ttccttgacg atcagggacc gccgcagggg tatcatgagc
      541 agggttccca gaaatccccc gaaaatcgcg acgatgaaga tgacggcata attgaagaag
      601 ccgcccgaat cggtcgtcga caggaagagg aaccccggca gggtaaagac gataccggcc
      661 gcgatgcttt cgctcgcact gcccgtcgtc tggatgatat tgttctcgag gatggttgtc
      721 ttcaggagcc gccgcccgag cgtgatggcc agcactgcga taggaatgga ggcggagaca
      781 gtcaggccgg ccttgagcgc caggtaaacc gtggacgccc cgaagataat gccgaaaatg
      841 ctgccggcga gcagcgcgcg gaacgtgaat tccttcattt cggtttgcgc gctgacgaac
      901 ggttcaaatt tcttgttggt catttctcac cggttttttc gcgtactacc cggttcagcc
      961 atttgatccg gctaaagatc aatgccgatc cgaggacgcc ggccacgaac cagataagga
     1021 aatagattcg tttgtcgggg aatttgtccc aggaactcgt catcatcccg gcgaccttgc
     1081 tcccgagcga catcgtcagg aaccagccgc ccatcaacag cgaggtcaat cgcgccggcg
     1141 ccagcttgga tacgagcgac aggcccatgg ggctcaggaa gatctcggcg gcagtgatca
     1201 cgaaataggt cccccataac caggccggcg ataccttata ttgatatatg gaggggacgg
     1261 acatcacggc gaagaccatc accagggaag aaacgccgga aaggaagagc gcgatgccaa
     1321 atttcgccgg tgtggtgggt tcgcggttac gccttcgcag ccaggtcagc agtccgacga
     1381 gcagcggggt cagcaatacg ataaataatg gaccgatcga ctggaactcc tccgtattcg
     1441 caagcttcag gccggtaccc ggcggcggcc atttgtcgcg cggcaggttc cgaaaataga
     1501 ggtccgggcc catggtcttg ataatatggc cgctgtcatc cctcaccggc gccatgtacg
     1561 cattcatcga atcgaccttt cgcggtgtcg tattgacggt ttgcaggaaa tccatgaaat
     1621 tagcgccctt ctccatccag cgggtggtga tgctccggtc ggtatgccgt tccgtccaaa
     1681 gggtatagcc cgtgaagttt tgatagtaca aggaccagaa gatgaccgaa atggcaaaga
     1741 tgaacagcag cgcgccgatc cccttcctgt cttcacctct cgcccggacc cagagtgaca
     1801 ggaagaagcc gagaatcggg atgcacgcaa agacgaaggc atcgttcgag ggcgatccca
     1861 tcagggtcgt atggagcaat ttcccgggca gccaatagcc gatgatggcg gcggtgatgg
     1921 ccggaacaaa aacaaataaa ctgatctccc ccagcgtcat gtcctccctt agggactgtt
     1981 tgcggacgtc cccttccttc acgtgccgca gattggaagc caggttgatc agtccaatga
     2041 ccatcccggc ccccgcggcc ccgaaggcat atccccagcc atacttattg cgcaggtagg
     2101 cagccacgaa attgcagagc agggatccga tattgatccc catgtaaaag atattgtagg
     2161 cattgtcctt gagcggcttc agctcctctt tattgtaaat atttcccagc agggtgctga
     2221 tattcggctt gaagaaccca ttgccgatca cgaccaggcc aagggagatg tacatggccg
     2281 tgtttccggg gatgatcagg ccgaaataac ccgccgccag aaagacaccc ccgataaaaa
     2341 ccgagcggat atagccgaaa taccggtcgg cgatcatccc gccgacaaac atcggaagcc
     2401 agatcagggc gaggaaagtt ccggtaaggt cagaagcatc tttttcggaa aaacccttgc
     2461 ccccggtctt gttatccatc aaataaatga gcaaaatacc cgcgagcaaa taataagcga
     2521 accgttccca catttctgtc ataaaaagca catagaggcc tctcggatgt ctggccctgt
     2581 gggcggtggc ttggctcatg caatcttggg ttatgccgga accgcgtccg gcgggtccgt
     2641 tggccgctaa aataaattct tttttacaat tccgcctgaa attcgaattt cggggctgta
     2701 tgaaaatcct attgttaggg tcgggaggca gggaacacgc cctggcgtgg aaactggctc
     2761 aaagtccgct ttgtacctcg cttcacattg caccgggcaa tcccggcacc gccaggtgcg
     2821 ggaccaacgt cgatctgtcc ccgctcgatt ttgaagggat tcgccggcat tgtaccgcgg
     2881 cggggatcgg gatggtggtg gtcgggcccg aagagccgct ggtgaaaggc attgtggatt
     2941 ttttccttgc cgatccggcc ctgcggggga tcccggtgat cggaccgtcg gcagcgggcg
     3001 cccagctcga aggcagcaag gcgttctcta agaagttcat ggatcgccac ggcattccaa
     3061 ccgctgccta ccgggagttc aatcacacgg atttcgcggc gggcatcgcc tacctgcagc
     3121 agcattcgct tcccgtagta ctgaaagcgg atgggcttgc cgcgggaaaa ggcgtagtga
     3181 tctgccagaa ccatgtggag gcggtggcgg agttcgagct gatggtgcag g
//
LOCUS       DATGKE010000058         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_309563, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000058 DATGKE010000000
VERSION     DATGKE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309563"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..2071
                     /locus_tag="VKU83_00945"
     CDS             524..2071
                     /locus_tag="VKU83_00945"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLI92138.1"
                     /translation="MRQKLQLKLLPAEANDPDIIRERIAQAAGRQVQDVSGFQLERRS
                     IDARGRHPHILLTLEAFIGEPFIERMPERISFPDVGKAGRQVIVVGAGPAGLFAALRL
                     IELGVRPVVLERGKDIRSRRRDLAALNKEGVIDPDSNYCFGEGGAGTYSDGKLYTRST
                     KRGDIKRILQLFVQFGASPSILVDAHPHIGTNKLPHIIHAMRQQILASGGVFLFEKKL
                     VDFVLHADRILSVRTADGDTISGSAVILATGHSARDIFQLLDQKRIRIEVKPFALGVR
                     IEHPQELIDLIQYNTPSPDLPPASYSLVEQIDGRGVFSFCMCPGGIIAPASTDNGELV
                     VNGWSPSKRNNPFANSGMVVTIEEKDMQAFRKHGPLAGMYFQQAVEEKAFAAGGGRFV
                     APAQRMTDFVESHLSADLPRCSYPPGLHAALLGEVLPDFVSEALRKGLRIFGAKKRGY
                     FTREAVIVATESRTSSPVRVPRDPEKWHHPTLRNLFPCGEGAGFAGGIVSAAMDGERA
                     AEAAAQG"
     gene            2148..3110
                     /locus_tag="VKU83_00950"
     CDS             2148..3110
                     /locus_tag="VKU83_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI92139.1"
                     /translation="MPILQVSDLQKNFSSQIAVNRVSFQLDNNSIFGLLGPNGAGKTT
                     LIRMITGIFFPDDGQILFQGRKFDPATDIVQIGYMPEERGMYKKMKIGEQAIYLARLK
                     GLSSQEAHARVREWFTKFEMQSWWNKKVEDLSKGMQQKLQFVITVLHEPKLIILDEPF
                     SGLDPVNSNLIKDEIYNLARKGSSIIFSTHRMEQVEEICDHIILMNKGEKILDGSVAG
                     VKQQFKENLFAVGVESADGWSPANGAFEVVGRRDDQWVVKIRQGHRPNDVLRYFIDQG
                     RSINAFTEILPSLSDIFIRLVEGTPLARQFQPVGGPAVTRPNAS"
     gene            3170..>4476
                     /locus_tag="VKU83_00955"
     CDS             3170..>4476
                     /locus_tag="VKU83_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92140.1"
                     /translation="MSKMWLITKREYLSRVRRKTFLISTFLLPVVFIVFIAGAVLIQL
                     KTKSHHKIAVVDANGFFRDYLRGDSTISFIFSPEADTANYEAKGCSAVLIIPKLADSG
                     KTVCRLKFKKSLGIEQTTALEDHVHQAVMDHMIYAQTAVSRAKLDSIRSQADITDVRS
                     FEDLGPTAKASSQTSAFAIGYVSGILIYLLTFVYGAAVMRGVMEEKTNRIAEVMVSSV
                     RPFELMWGKITGIGAVGLTQFLLWVILLGAIGMVAQAVVPHEALNNIPQSGPGSMSAA
                     AGAMKAGSPGFNLTNFIINANLGLVLFCFLFYFIGGYLFYSALFAAVGSAVNEDPQEA
                     QSLMLPITLPVVFSFVIMTAVVQDPTGPLATWASIIPFSSPIVMMARIPSGVPGAVPY
                     WQLGLSMLMLVLGFLGTTWLAGKIYRVGILMYGKKPTWKIMWK"
BASE COUNT         1024 a         1199 c         1239 g         1014 t
ORIGIN      
        1 tttgtggaat aaaaaatttt ttattcacct gtttttctcg cccggaatac aattcaattt
       61 aaggataaga aaatcgatga agcgatgcaa agccggctgc ataacaaccg cccccacgtg
      121 gcagaaagcg acaatagtgg atccagatac tcggcagttg gcctggccga aacaacctaa
      181 acttactttt tatgaagaag gaggaactga gcttcgatgt gaacgaacct ttcttcatat
      241 atgtagagtt ttgagtgaag tcattgaaga tagcaaaata atttgattgc atttttttaa
      301 acccaaattt tttttcaaaa aaaaattgtt atccgtaggt agtatatttt tcatcgggca
      361 tctccccttt aggaaaatct acggtgtcgg attatatctc aaacaaccat aactatttat
      421 agtacttttg gtgccggacg gaccaaattt ccgttcgtcg aagacgataa atacgcgctc
      481 ggtgaaacat catccgtcat tgtttcacga ggcgaggcac acaatgagac aaaagctcca
      541 actgaaactt ttgcccgcgg aggcaaacga tcccgacatc atccgggagc ggattgcaca
      601 agccgcgggc cgtcaagtgc aggatgtatc cggctttcag ctcgagcggc gctcaatcga
      661 cgccaggggc cggcatccgc acatcttgct tacactggaa gcctttattg gcgagccttt
      721 catcgaaagg atgcccgagc gcatttcttt tcccgacgtc ggaaaggctg gtcgccaggt
      781 gatcgtcgtg ggcgcaggtc cggcgggcct ctttgccgcg cttcgtttga tcgaactggg
      841 cgtccggccg gtagtccttg aaagaggcaa ggacattcgc tcgcggcgtc gtgacctggc
      901 cgcgctcaat aaagaaggcg tcatcgatcc ggacagtaat tattgtttcg gtgaaggcgg
      961 cgcgggaacc tatagcgacg gcaaactata cacccgcagc accaagcgcg gtgatatcaa
     1021 aaggatattg cagctcttcg ttcagttcgg cgcctcgcct tccatcctgg tggatgcgca
     1081 tccgcatatc ggcaccaata agttgccgca tatcatccat gccatgcggc aacagatcct
     1141 tgccagcggc ggcgtttttc tgtttgagaa aaagcttgtc gactttgttc tacacgccga
     1201 tcgtatcctt tcggtccgaa cggcggacgg cgacactatc agcggcagcg cagtcattct
     1261 ggcaaccggt cactcggcgc gggatatctt tcaactcctg gatcaaaaac gtatccggat
     1321 cgaagtcaaa ccattcgcgc tgggtgtccg gatcgagcac ccgcaggaat tgatcgatct
     1381 tattcaatat aatacgccgt caccggattt gccgccggca tcctatagtc tcgtggaaca
     1441 aatcgacggt cgcggcgtgt tttcgttttg catgtgtccg ggcggcatca tcgcccccgc
     1501 ctcgaccgat aacggggagc tggttgtcaa cggctggtcg ccgtcaaaac gcaacaatcc
     1561 tttcgccaac tcggggatgg tcgtaaccat tgaagaaaag gacatgcagg ctttccgcaa
     1621 acacgggccg ctcgcgggca tgtattttca gcaggcggtc gaagagaaag cgttcgccgc
     1681 cggtggcgga aggttcgtag cgccggcaca acggatgacg gattttgtgg aatcccacct
     1741 ttccgccgac ctgccgcgct gctcctaccc tcccggcctc catgccgcat tattgggcga
     1801 ggtgcttccg gattttgtga gcgaagcgct ccggaaaggg cttcgcatct tcggcgcgaa
     1861 gaagcgcggc tatttcaccc gtgaggcggt tatcgtagcg acggaatcca ggacatcatc
     1921 cccggttcgt gttccgcgtg atccggagaa atggcaccat cccacgctcc ggaacctttt
     1981 tccctgcggc gaaggcgccg gattcgccgg cggaatagtt agcgctgcca tggatgggga
     2041 gcgggcggcc gaagcggcgg cgcaagggta atatttgttc agccgccgcg acgaattgac
     2101 gtccggcacc gccaaatgat gtatttttag ctctaaacga actgccgatg cctatcctcc
     2161 aggtctccga ccttcaaaaa aatttctcct cgcaaattgc cgtcaaccgg gtgagcttcc
     2221 agcttgacaa taactcgatt ttcggcctcc tcggcccgaa cggagcggga aagactacgc
     2281 tcatcaggat gatcaccggc atcttctttc cggacgacgg tcaaattctc ttccagggcc
     2341 gcaagttcga cccggcgacc gatattgtcc agatcggcta tatgcccgag gagcggggaa
     2401 tgtataaaaa aatgaagatc ggggagcagg cgatctatct ggcccggcta aaaggattaa
     2461 gcagccagga ggcgcatgcc cgggtcaggg aatggttcac caagttcgag atgcaaagct
     2521 ggtggaacaa aaaggtggag gacctcagca aaggcatgca gcagaaattg cagtttgtca
     2581 tcaccgtgct ccacgagccc aagctgatta tcctcgacga gccattcagc ggcctggacc
     2641 cggttaactc taatcttata aaagacgaga tctataacct ggcccggaaa gggtcatcga
     2701 tcattttcag cacgcaccgg atggaacagg tggaggagat ctgcgatcat atcatcctga
     2761 tgaataaggg cgagaagata ctggatggat cggtggccgg ggtgaagcaa caattcaaag
     2821 aaaacctttt tgctgtcggc gtcgagtccg cagacggctg gtctcctgcc aacggggctt
     2881 tcgaagtagt cgggcggcgt gacgaccaat gggtcgtgaa gatcaggcaa ggccaccggc
     2941 caaacgatgt gctgcgatat ttcatcgatc agggcaggag catcaatgct tttaccgaga
     3001 tcctgccttc gctgagcgat attttcatcc gtctcgtgga gggaacgccg cttgcgcgac
     3061 aatttcagcc tgtcggggga ccggcggtca cccggccaaa tgccagctaa tcaaccgatg
     3121 tgacgaaggc gaatgttcag gccggagtcg caaccaaaaa catactacga tgagcaaaat
     3181 gtggttaatt accaaacgcg aatacctgag ccgggtgcgg cgaaagacct ttcttatttc
     3241 aaccttcctg ctgccggtcg tctttattgt ttttattgcc ggcgcggtac tgatacaact
     3301 caagacaaaa tcccaccata agatcgcggt tgtcgatgcg aatggttttt tccgcgacta
     3361 cctcagaggg gattcgacga tcagctttat attttcgccg gaggccgata cggccaatta
     3421 tgaggcaaag ggctgctccg cggtgctcat catcccgaag ctggccgaca gcggcaagac
     3481 cgtttgccgg ctgaagttca agaaatccct tggcatcgag caaacgacgg cgctcgagga
     3541 ccatgtccac caggcggtga tggatcatat gatctacgcg cagacggcgg tcagccgcgc
     3601 gaagctcgac tccatccgga gtcaggcgga cattaccgac gtcaggagtt tcgaggacct
     3661 cggaccgacc gcgaaggcgt ccagccagac ctcggccttc gccatcgggt atgtcagcgg
     3721 cattctcatc tatttgctga cctttgtata tggcgccgcg gtgatgcgtg gcgttatgga
     3781 agaaaaaaca aaccgtatcg ccgaagtgat ggtgagctcc gtccgcccct tcgagctgat
     3841 gtggggcaag attacaggga tcggcgccgt cggcctaacc cagttcctct tgtgggtgat
     3901 cctcctgggg gcgatcggca tggtggccca ggctgtcgtc ccgcacgaag ccctgaacaa
     3961 tattccgcag tcagggccgg ggagcatgtc cgccgccgcg ggcgcaatga aggcgggctc
     4021 tcccggcttt aatctcacca atttcatcat caacgccaac cttgggctgg tgctattctg
     4081 ttttttgttt tatttcatcg gcggttattt attctacagc gccctgtttg ccgccgttgg
     4141 cagcgcggtc aatgaagacc cgcaggaggc gcaatcgctg atgctgccga tcactttgcc
     4201 cgtggttttc tcgtttgtga tcatgacagc tgtcgtacag gacccgaccg ggccgctggc
     4261 aacctgggcg agcattattc cgttttcctc cccaatcgtc atgatggcga ggatcccatc
     4321 gggggtgccg ggcgcagtgc cgtattggca actgggtctt tccatgctta tgctggtgct
     4381 ggggtttctg ggtacaacct ggctggcagg aaagatctac cgcgtcggca ttctaatgta
     4441 tgggaagaaa cccacctgga agatcatgtg gaaatg
//
LOCUS       DATGKE010000059         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1125178, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000059 DATGKE010000000
VERSION     DATGKE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125178"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..402)
                     /locus_tag="VKU83_00960"
     CDS             complement(58..402)
                     /locus_tag="VKU83_00960"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HLI92141.1"
                     /translation="MAMSSTIDWGDFEKIDVRVGTIVEVNDFPKAKRPAWQLSIDFGE
                     LGLKRSSAQLTAFYSREALLGRQVLAVVNFPPKQIANFSSECLVLGVYTDKKEVVLIV
                     PDRKVENGWKVG"
     gene            442..867
                     /locus_tag="VKU83_00965"
     CDS             442..867
                     /locus_tag="VKU83_00965"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLI92142.1"
                     /translation="MKRTATAVWNGSGKDGKGNLTTQSTTLKQTQYSYKSRFEEGVGT
                     NPEELIAAAHAGCYAMALAFALQNAGFTPDSLETTSTITLDVSGQAPTITASHLVLKA
                     KIPGISKDKFQELAHGAEKGCPVTRVLKAEVSLEATLLA"
     gene            1042..2466
                     /locus_tag="VKU83_00970"
     CDS             1042..2466
                     /locus_tag="VKU83_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI92143.1"
                     /translation="MSNKHSKKTSRREFIRNTAVASTGFFIVPRHVLGRGFRAPSDML
                     QLAAIGAGGKGADDLRHFFGSGKVNVAYLCDVDDRQAADSVKKYTKAKYYKDFREMLD
                     KEHKHIDAVSVSCPDNCHAVAAMAAIMLGKHVYVQKPMTHDIYEARMLTEAVKRYKVV
                     AQMGNQGASNDGVRRMQEWYNAGLIGDATDIHVWTNRPVWPQGGPTPTSTDPVPPELN
                     WDLWQGPAAAAQYHKEYLPFNWRGWWAYGTGALGDMGCHLIDPAFKTVGLGYPSEVEC
                     SVANVVEEMWHVAYHPESCPIASIVKMKFPGKPGKPDVNLHWMDGGLLPDRPEELGPD
                     EVMGDTDGCGVIITGTKGKMMCGTYSSRATLLPTSRTDGNSVPQTIPRVPEGHYVQWV
                     NACLAGYGKNELSSPFDYAGPLTETILMGNLALRSHNVKDASGKFTGRKKLLWDAKNM
                     KITNYDEANEFVRRNYRQGWSLGI"
     gene            complement(2532..3449)
                     /locus_tag="VKU83_00975"
     CDS             complement(2532..3449)
                     /locus_tag="VKU83_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLI92144.1"
                     /translation="MNLVDLTTVYPWMNPMQENKEIQALFHLIDDPDQEVFDSVSTRI
                     VSYGRGIIPNLENLWENTISNDVQERIELLIHRLHYHDLTEDFLHWKNSSYQDLLTAS
                     LLVAKFQYPELVTTPVLQEVEKLRRNIWLELNSYLTPLEQVNVLTSILYNYYGLKGTE
                     VAYQHPEEFMINKLIETKRGNAITNGILYLLLSELLDIPVKAINIPRQFVLAYIKPYY
                     DENPEKLSPQQRIEFFIDPMSGQVFSHKDVENYFKRVSVPPVASYFKPLTHNRIIQTA
                     LEEFAKCFDDERSQYKQKELLDLARLLND"
     gene            complement(3497..>4474)
                     /locus_tag="VKU83_00980"
     CDS             complement(3497..>4474)
                     /locus_tag="VKU83_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HLI92145.1"
                     /translation="VKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGVRRDLRVLL
                     LKTNKIEQLDEQETTGAEKSKLFPTDLGLVVTDFLKQYFDDIMDYNFTARIEEEFDEV
                     AQGKMLWSKMIDDFYNPFKKDVENTIENAERIRGERELGVEPESGKKVIARMGRYGPM
                     VQIGDVSDEEKPRFAKLKNTQSIETISYDEAMELFRLPRSLGSFEDKEVTVNIGRFGP
                     YLAHDGKFYSLGKDFDPYTISFDQATPLIVEKRAAKEQRTIKVFEKEKIQLLRGPYGP
                     YIKQGLRNFKLPKEKQDVAADLTLDEVKAIIEEVKANPPKRVPRRRKGN"
BASE COUNT         1007 a         1227 c         1177 g         1063 t
ORIGIN      
        1 tatctcgagc gtcgcagcca tattcgaagg ggttaaagtc gattccaact tcgttgctca
       61 tccgaccttc catccattct ccaccttccg gtccggaact attaaaacta cttctttttt
      121 gtcagtatac acaccgagca cgaggcattc gctggaaaaa ttggctattt gctttggcgg
      181 gaagttaacg acggcaagga cctgccggcc cagcagcgcc tcccggctgt aaaacgccgt
      241 gagctgggct gaagaccgtt taagccccaa ttcaccgaaa tcgatgctta gttgccaggc
      301 cggccgtttt gctttcggga aatcgttaac ttcgactatt gttccgaccc ggacatctat
      361 cttttcgaaa tcaccccagt cgatggtaga tgacatagcc attagcgcat tcattttatc
      421 atttattaaa tataccattt tatgaaacgt acagcaacgg ccgtatggaa cgggtccggc
      481 aaggacggga aaggcaacct gacgacacag agcaccacct tgaaacaaac gcagtattcg
      541 tacaaatccc ggttcgaaga aggcgtcggc accaatccgg aagagctgat cgccgccgca
      601 cacgccggat gctatgcgat ggcacttgcc tttgcccttc aaaacgcagg atttacgccg
      661 gacagtctcg agactacatc cacgattacc ctcgacgtga gcggccaggc tccgacgatc
      721 acggcttcgc atctcgtgct aaaggctaaa attcccggca tcagcaagga caaattccag
      781 gagctggcgc atggcgccga gaaaggctgc ccggttacgc gggtcctcaa agcggaagtc
      841 tcgctcgaag cgacgctgct ggcttgagtt ctggccgaat catggcgacg cgcaacgcgt
      901 cgccggacat taaaatctca ttagaatgct accgcaaaaa tttgatcagc gggggctttt
      961 gtattgttca aacccgtaac tttctgtttc aaacatctac caacagtcta tctcttcatc
     1021 tcttgtttta attcgaagca catgagtaac aaacattcga aaaagacgtc ccgccgcgaa
     1081 ttcattcgca acacggcggt ggcctcaaca ggttttttta tcgtaccgcg ccacgtgctg
     1141 ggtcgtggct tccgtgctcc cagtgatatg ctccaactcg ccgccatcgg ggccggcgga
     1201 aaaggcgccg acgacctaag acattttttc gggagcggca aggtgaacgt cgcctacctc
     1261 tgcgatgtcg atgaccggca ggcggccgat tcggtgaaga agtacacgaa ggccaaatac
     1321 tataaggact tccgtgaaat gctcgacaag gagcacaagc atatcgatgc cgtatccgta
     1381 tcctgtcctg acaattgtca tgcggtcgcc gcaatggccg caatcatgct cggcaagcat
     1441 gtgtacgttc agaaaccgat gacgcatgac atctacgagg cgcgcatgct gaccgaagcg
     1501 gttaaacgct ataaggtggt cgcgcagatg ggtaaccagg gcgcttccaa cgatggcgtc
     1561 cgccgcatgc aggaatggta taacgcaggt ttgatcgggg atgcgacgga catccatgtg
     1621 tggacaaacc ggccggtatg gccgcagggc ggaccgaccc ctacgagcac cgacccggtt
     1681 ccaccggaat tgaattggga cctctggcag ggaccggcgg cggccgccca gtatcacaag
     1741 gaatatcttc ccttcaactg gcgcggctgg tgggcctatg gcaccggcgc tctcggcgac
     1801 atgggctgcc atctgatcga tccggcattc aaaaccgtcg gccttggata cccttccgaa
     1861 gtggaatgca gtgttgccaa cgtcgttgaa gaaatgtggc atgtggccta ccatcccgaa
     1921 agctgcccga tcgcatccat cgtaaagatg aaattccccg gtaagcctgg gaagcccgat
     1981 gtgaacctgc actggatgga cggcggcctg cttccggacc ggccggaaga acttggaccc
     2041 gatgaggtga tgggcgacac cgatgggtgc ggcgtcatca tcaccggcac aaaggggaaa
     2101 atgatgtgcg gtacctatag cagccgcgca accctgcttc cgacctcgcg tacggacggg
     2161 aacagcgtcc cgcagacgat ccccagggtg ccggaaggcc actatgtcca gtgggtgaac
     2221 gcctgtcttg ccggctatgg taagaacgag ctgagctcac ccttcgacta tgccggaccg
     2281 ttgaccgaaa cgatccttat gggcaacctt gccttgcgca gtcataatgt gaaggatgcc
     2341 agcggcaagt tcaccggccg gaagaaactt ctctgggacg cgaagaacat gaagatcacc
     2401 aactatgatg aagcgaacga gttcgtgcgg cgtaactacc gccagggctg gtcgctcggc
     2461 atataaaggt tcggccggcg ggtcttaccc gccggcccga tccggcacgc cacgcgtgcc
     2521 ggatcttttc attaatcatt taagagtctc gccaggtcca gcagctcttt ctgcttatac
     2581 tggctccgct cgtcatcgaa gcatttcgcg aactcttcca gcgccgtctg aatgatgcgg
     2641 ttatgcgtca gcggtttgaa gtaggacgca accggaggca cggacacccg tttaaaatag
     2701 ttctccacat ccttatgcga aaatacctgc ccgctcatcg gatcgataaa aaactcgatc
     2761 cgctgctgag gacttagctt ctccggattc tcgtcgtagt aaggtttgat atatgccaac
     2821 acgaattggc gggggatatt gatcgctttc accggaatat ccagcagctc gctcaacaac
     2881 aaatacagga taccgttggt aatggcattt ccgcgcttgg tttcgatgag cttattgatc
     2941 atgaattctt cggggtgctg gtacgcgacc tcggtaccct tcaggccata ataattataa
     3001 aggatgctgg tcagcacgtt tacctgttcc agtggagtca gataactgtt cagctccagc
     3061 cagatgttcc ggcgaagttt ctcaacctcc tgcagcacgg gcgtcgtcac cagctcggga
     3121 tactgaaatt tggcgacgag cagcgaagca gtgagcagat cctggtagct gctgttcttc
     3181 caatgcagga agtcttccgt aaggtcgtgg taatggagtc tgtggatcag gagctctatg
     3241 cgctcctgga cgtcgttgct gatggtattt tcccaaaggt tttccaggtt ggggataatg
     3301 cctctgccgt aactgacaat ccgggtgctg accgaatcga aaacctcctg gtcggggtca
     3361 tctatcaggt ggaaaagggc ctgtatttct ttgttttcct gcatggggtt catccaagga
     3421 tatacggtag taaggtcgac caagttcata agaatttggt aaaattctta tccacaataa
     3481 cgaccggaat gttcatttaa ttgcccttcc tgcggcgcgg aacccgcttt ggcggattgg
     3541 ccttgacctc ctcgataatg gctttgacct catcgagcgt aagatctgcc gcaacatcct
     3601 gtttttcctt tggaagcttg aagttgcgca gcccctgctt aatataggga ccataggggc
     3661 cgcggaggag ctggatcttt tccttttcga acactttgat cgtccgctgc tccttggccg
     3721 ctcttttttc gacgatgagc ggtgtggcct ggtcaaaact aatggtatac ggatcaaaat
     3781 ccttgccgag ggaatagaac ttcccgtcat gggcgagata cggcccgaag cggccgatat
     3841 tcaccgtgac ttctttatct tcaaaggaac cgaggctgcg gggcagccgg aagagttcca
     3901 ttgcttcgtc ataactgatc gtctcgatgc tttgggtgtt ctttagcttg gcgaaccgcg
     3961 gtttctcctc atcgcttacg tccccgatct gcaccatggg tccgtagcgc cccatccggg
     4021 caatcacctt tttaccgctt tccggctcta cgcccaattc gcgttctccc ctgatccgtt
     4081 cggcgttttc gatcgtattt tcaacgtcct tcttaaaggg attgtaaaaa tcgtcgatca
     4141 ttttactcca taacatctta ccctgcgcca cctcgtcgaa ttcctcctcg atccgggcgg
     4201 tgaagttata atccatgata tcgtcgaagt actgcttgag aaaatcggtt acgacgagtc
     4261 cgaggtccgt agggaagagc ttggactttt cggctccggt agtctcctgc tcgtccagtt
     4321 gttcgatctt gttcgttttc agcagcaata cccgcaggtc ccgccgtacg ccttccttgt
     4381 cgcgcttctc aacgtaaccg cgcttaagga tggtagagat ggtaggggca taggtcgagg
     4441 gccttccgat gcccagctct tccagtttct tgac
//
LOCUS       DATGKE010000060         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1132419, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000060 DATGKE010000000
VERSION     DATGKE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132419"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="era"
                     /locus_tag="VKU83_00985"
     CDS             complement(<1..383)
                     /gene="era"
                     /locus_tag="VKU83_00985"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLI92146.1"
                     /translation="MKAGFVSIFGKPNAGKSTLLNALMGEKLAIVSPKVQTTRHRIKG
                     ILTAAGYQIIFSDTPGIIEPRYKLHEKMMQAVRNSLEDADLALLLVDIRDDWEENDRI
                     FDALRLRVPGILALNKSDGVADERI"
     gene            complement(468..539)
                     /locus_tag="VKU83_00990"
     tRNA            complement(468..539)
                     /locus_tag="VKU83_00990"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Met,seq:cat)
     gene            complement(615..1349)
                     /locus_tag="VKU83_00995"
     CDS             complement(615..1349)
                     /locus_tag="VKU83_00995"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLI92147.1"
                     /translation="MINFMIMGRIFAIFVGAMLACSARAQQTVIADPNAQLRPVKGYH
                     GVRVSSAINLYLSQGQEEKVVVSAKDVKLRDRIVTEVVDGILVIRLDVKPWHRLGNNK
                     LKAYVSFTALDQISASGASDVYVDGVISGDRLSLDLSGASDFKGAIKVNELLLDQTGA
                     SDSQITGQVGGQATIRTSGASDVKAYDLVVDNCSVHASGASDVRITVNRQLDADLSGA
                     SSVYYKGEGVIRESHSSGASEVAHKG"
     gene            complement(1358..2077)
                     /locus_tag="VKU83_01000"
     CDS             complement(1358..2077)
                     /locus_tag="VKU83_01000"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLI92148.1"
                     /translation="MKNSFLLAALLLTGAAATAQTSKIINDPNAQKRSATGFHAIEVS
                     GGIDLYLSQGGDEAVAVSAADPESRDRIVTVVQGGVLHIYMEDHDFHWRWRNRRLRAY
                     VSCKTLDDLTSSGGSDVYIDDAIRSERLEVHLSGGGDLHGKLQVGDLTVGMSGGADSY
                     VSGTATRLRIHVSGGGDFHGYDLAADSCEAHASGGGDIYVTVNKSLNASVSGGGDIRY
                     KGNATIRDIHTSGGGSIARRD"
     gene            2235..>2724
                     /locus_tag="VKU83_01005"
     CDS             2235..>2724
                     /locus_tag="VKU83_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92149.1"
                     /translation="MLKFTAIVLAVPGLLAASAPTACAQRGSTARPGGPSAPAIQVSH
                     RMPAIVPGGSPADKRPIRPADLYRLPGISDPQPSPDGKWISYTLSTIDSLKDTRNSDI
                     WMISWDGTQDIQLTNSPEGESHARWSPDGRYLSFLSGRQASGADGNIKGSQVWLMDRR
                     GGE"
BASE COUNT          559 a          867 c          714 g          584 t
ORIGIN      
        1 tcgatgcgtt catcagccac cccatcgctc ttgttgagcg caagtattcc gggaacgcgc
       61 aaccgcaaag cgtcgaagat gcggtcgttc tcctcccagt catcccggat atccaccagc
      121 agcaaggcaa gatcggcgtc ctccagggaa ttccttaccg cctgcatcat tttttcgtgc
      181 agcttatagc gcggctcgat gatccccggc gtgtcggaga aaatgatctg ataaccggcg
      241 gccgtcagta tccctttgat ccggtggcgt gtcgtttgca ccttgggcga gacaatagca
      301 agcttctctc ccatcagggc gttcagcaaa gtgctcttgc cggcatttgg cttgccaaat
      361 atgctcacaa atcctgcttt cattgctaca tgattccacg caaatataaa aaagggcccc
      421 tttgaggccc ttgatgtcaa tacgatggac ccttccaggg tcctttgttg cgaagggtgg
      481 gatcgaacca ccgaccttcg ggttatgagc ccgacgagct accgctgctc tacttcgcga
      541 tgtggacggc aaatttacgc ccaattccct accctaccaa ataaaaatgg cgtgctgtat
      601 ccgccggccg ggggttagcc cttgtgggcc acctcgctgg cgccgctcga atgggactcg
      661 cgtataaccc cctcgccctt atagtaaacg ctgcttgcgc cggagaggtc ggcatccagc
      721 tgcctgttga ccgttatccg gacatccgac gcacccgaag catgaaccga acagttgtcc
      781 accacgagat cataagcctt gacgtcgctt gcaccggagg tccggatcgt agcctgtccc
      841 cctacctgcc cggtgatctg cgagtcggat gcgccggtct ggtcgagcaa caactcgttg
      901 accttgatgg cgcccttgaa atcgctcgct cctgagaggt ccagggaaag ccggtcgccc
      961 gaaatcacgc cgtcgacgta cacgtcgctt gcgccggatg cgctgatctg atccaaagcc
     1021 gtaaacgata cgtaggcctt aagcttgtta ttccccaatc gatgccaggg tttaacgtcg
     1081 agccggatga ccagtatccc gtccaccacc tccgtgacaa tccggtctcg caatttcaca
     1141 tctttcgcgc tgaccaccac cttctcttcc tgtccctggc tgaggtagag attgattgcg
     1201 ctggagacgc ggaccccgtg ataccctttt accggccgca gctgcgcatt cggatccgca
     1261 atgaccgtct gctgcgccct ggcactacat gccagcatgg cccccacaaa aatggcaaag
     1321 atcctgccca tgatcataaa gttaatcatt tcgaatttta atcccttctg gcaatgcttc
     1381 cgccgccgct ggtatggata tcccggatgg ttgcgttgcc cttataacgg atatcgccgc
     1441 cgccgctgac cgaggcattc aggctcttat taaccgtcac ataaatatcg ccgccgccgc
     1501 tggcatgcgc ctcacagctg tccgccgcca gatcatatcc atggaaatcg ccgccaccgc
     1561 tcacatggat ccgcagccgg gtcgcggtgc cgctgacgta cgagtcggcc ccgcccgaca
     1621 ttccaaccgt caggtcgccg acctgcaatt tgccgtggag atcaccgccg cccgacagat
     1681 gcacttccag cctttccgat ctgatcgcat cgtcgatata aacatccgat ccccctgacg
     1741 atgtcaggtc gtccagcgtt ttacatgaaa cataagcacg caaccggcga ttccgccacc
     1801 gccagtggaa gtcatgatcc tccatgtaga tatggaggac gccgccctgc accaccgtaa
     1861 cgatccggtc cctggattcg gggtcggccg cgctcacggc gaccgcttcg tcgccgccct
     1921 ggctcaggta gagatcgatg ccaccgctga cctcaatggc atgaaagccg gtcgcggagc
     1981 gtttttgcgc attgggatcg ttgatgattt tcgacgtctg ggccgtggcg gccgccccgg
     2041 tcagcagcag cgcagccagc aggaaagaat ttttcataat tcagcagttt ttttaatgac
     2101 ggaatggcgc cggcaatgtt gcactgccgt taatttgaga cgccccgggc cgggaaaagg
     2161 ttacaatgcg ggtatagaaa ttactcgtac ctttccttat ccgggtcatc ccgcgtcctg
     2221 ataactactg cattatgtta aaattcaccg caatcgtcct ggcagtcccg ggccttctgg
     2281 ctgccagcgc tccgaccgcc tgcgcacagc gtggaagcac cgctcgaccg gggggtccct
     2341 cagcgcctgc gatccaggtc agccatcgaa tgcccgccat cgtcccaggc ggctctccgg
     2401 cggataaacg ccccatccgg ccggcggatt tataccgcct tccgggaatc agcgaccccc
     2461 agccctcccc ggacggcaag tggatctcct acaccctcag caccatcgac tcgttaaagg
     2521 atacccggaa ttccgatatc tggatgatca gttgggatgg aacccaggac atccagctca
     2581 cgaatagtcc cgagggagaa tcgcacgccc gctggagtcc ggatggcaga tatttgtcct
     2641 tcctttcggg aagacaagca tccggggccg atgggaacat caaaggcagc caggtctggt
     2701 tgatggaccg gcgcggcggc gaag
//
LOCUS       DATGKE010000061         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_915948, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000061 DATGKE010000000
VERSION     DATGKE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915948"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VKU83_01010"
     CDS             <1..1423
                     /locus_tag="VKU83_01010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLI92150.1"
                     /translation="GKTLQALSFLRYLATRYPDETHLVVCPTSLLYNWEAEMKKFVPD
                     LDWYVYYGASRKLDESSFRRSRVVVTSYGMLRSDISELAAWRWGYVILDESHAIKNPA
                     SRVRKAVQQLQTRNRMALSGTPVQNSTFDLYAQLDFLNPGMLGSQEFFRTQFATPVDR
                     NGDKEAAARLRKLVYPFILRRTKEQVAKDLPDKTETILWCEMGEEQRRIYDRYREAYR
                     AGLLDRIGQEGIGKSAIHILEGLTKLRQICDSPVLANSSSPAGDEAPVAASAQLPHVS
                     AKLEELIREIAELFAAASCDERLGFDRESVYSGRSGTRQNSPPEHHKALVFSQFTSML
                     QLIRAALEEKGIPYLYLDGSVAAEHRKRIVAQFQEEEDTRIFLISLKAGGVGLNLTAA
                     DYVYLVEPWWNPAAEQQAIDRTHRIGQGKKVFAYRMICRDTIEEKILQLQEKKRGLAS
                     ELIGDEAGFVKQLTREDVAYLFS"
     gene            1452..2225
                     /locus_tag="VKU83_01015"
     CDS             1452..2225
                     /locus_tag="VKU83_01015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLI92151.1"
                     /translation="MRTVLTDLLDVADRYFLVAGSAFLLAYVVLKKRIAWRKIQPRFP
                     AAKDYRREIIDSTISILIFAMMPIVILRVPAIRMHTQLYGPPPERGWLYFFLVFPIMF
                     VIHDAYFYWMHRLIHQPKLFRLIHLEHHRSVNPSPWAAYAFGPIEAFLEALIFPIILF
                     TIPVTVWHVFIFFLLSIVYNVYGHLGFELYPKGFHRTLLGRWINTSVSHNQHHHYFKG
                     NYGLYFLWWDRWMGTIRKDYDQAFGEVTGRIKHAARTTG"
     gene            complement(2222..3076)
                     /locus_tag="VKU83_01020"
     CDS             complement(2222..3076)
                     /locus_tag="VKU83_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HLI92152.1"
                     /translation="MVQVGVFNNLVVKRQLNMGLILDDGAEGILLPKRFAPRNAKPGD
                     LLRVFVYHDSEDRLIATTQHPKGILGDIVLLRVVSLTPQGAFLDWGLMKDLFVPKAKQ
                     LTGMRLGGDYLVKIYLDEQTGRLAATEKFEPFLTNNNLTVTDNDIVTLTVIRRTDIGY
                     LCAINNRHTGVLHYNEVYRDIKVGDRFPGFIKNVLPANQSPSGETQIDVAAGRPGYAR
                     VEDAAAKVLRLLEENKGRLPYHDRSDPGAIYSFFGMSKKTFKMTTGKLYKERKIRITE
                     SGIELLTT"
     gene            3171..3680
                     /locus_tag="VKU83_01025"
     CDS             3171..3680
                     /locus_tag="VKU83_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLI92153.1"
                     /translation="MRRSIILSCVFFAACHGTNNPILRPGSSFANNSGSPKWVSLPDS
                     SRIKISDGTTIVVGAGYSRGNRAVDLDGEGMFMIRGGDRFVVTTKNLYIQGMGTKFRV
                     DAPRNKPGEEVDLLDGVMVIRKMYHSDLDSAQEVLHAGDMLMINREIDLMEKEKMDTD
                     ELHKAEAKF"
     gene            3729..4514
                     /locus_tag="VKU83_01030"
     CDS             3729..4514
                     /locus_tag="VKU83_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HLI92154.1"
                     /translation="MPRVVSYQIADNIDVRGLRTAYKEGIYLYDADELFYKMGEGRYV
                     YVFKYGVVCFMGYNEGEAGLYLEQVGPFCKNLFERRLSEEFEVETNAQENKFGYNKLE
                     ITKPDIEVIRLIMLNVSQSVALDYFSEQTNLLQEETNSHTLNLERRGRLKISGAALKK
                     YIGKTLHLKNRISENLYIFDSPPQTWEDENLNRIDQGLKRTFDLQERYRDIREGLEIV
                     RENLELFKDLLQYRNSTFLEWIVIVLVAAEVVNLLLDKLFKLK"
     gene            4767..>5012
                     /locus_tag="VKU83_01035"
     CDS             4767..>5012
                     /locus_tag="VKU83_01035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLI92155.1"
                     /translation="MSRETDKMFLGQAVALAREGILAGRGGPFGCVIVRSGEVIGRGS
                     NSVTSTNDPTAHAEIVAIREACVRLGDYQLAGCTLYAS"
BASE COUNT         1143 a         1229 c         1506 g         1134 t
ORIGIN      
        1 gggtaagacg ttgcaggcgc ttagtttcct tcggtacctc gcaacgcggt atccggacga
       61 gacgcacctg gtggtctgtc cgacctcatt actatataac tgggaggccg aaatgaagaa
      121 atttgtcccg gatctggact ggtatgttta ttatggcgct tcccggaagc tcgacgaaag
      181 cagttttcgc cggtcccggg tggtggtcac gagctatggg atgttgcgga gcgatatttc
      241 ggaactggcc gcgtggcgat ggggatatgt gatcctggac gagagccacg ctatcaagaa
      301 tccggcgtcg cgggtgcgga aagcggtaca gcagctccag acgcgcaacc ggatggcttt
      361 aagcggaacg cctgtccaga acagcacttt tgatctttac gcgcagctgg actttttgaa
      421 tccgggcatg ctcggcagcc aggagttctt tcgcacgcag tttgcgacgc ccgtcgaccg
      481 gaatggcgac aaggaggctg cggccaggtt gcggaagctg gtctatccct ttattttgcg
      541 acggacgaag gaacaagtag caaaggatct gcccgataaa accgagacga tcctatggtg
      601 cgagatgggg gaggaacagc ggcggattta tgatcgctac agggaggcat accgtgccgg
      661 cctgctggac cggatcgggc aagaggggat cggaaaaagt gccatccata tattagaggg
      721 gctgacgaaa ttgagacaaa tctgcgacag tcccgtgttg gcgaatagca gttcgccggc
      781 cggcgacgaa gccccggtgg cggcttcggc gcaattgcct catgtaagcg ccaagctcga
      841 agaactgatc cgggaaatcg cagagctgtt tgcggctgca tcctgcgacg agcgtttagg
      901 ctttgaccgc gaatccgttt acagtggccg ttccggcacc cggcaaaact cgcctccgga
      961 acatcacaag gccctggttt tttcgcagtt cacttcgatg ctccaactaa tccgggccgc
     1021 gctggaggag aagggcattc catatctgta cctcgatggg agtgtggcgg ctgagcaccg
     1081 gaagcggatc gttgcacagt tccaggaaga agaagatacg cggattttcc tcatcagcct
     1141 gaaggcaggc ggggtagggc tgaaccttac cgcggccgat tatgtttacc tggttgaacc
     1201 ctggtggaac cctgccgcgg agcaacaggc gattgaccgc acccatcgga tcggtcaggg
     1261 aaaaaaggtt ttcgcctacc ggatgatctg ccgggatacc atcgaggaaa agatcctgca
     1321 gctgcaggaa aagaaaaggg gtctggcaag tgagctcatc ggcgacgaag ccggattcgt
     1381 aaagcagctg acgcgcgagg atgttgccta tttgttcagc tagggtactc gtaaaataat
     1441 tatatttgga agtgcgaacc gtactcacgg atctcctgga tgttgccgac cggtatttcc
     1501 tggtcgcggg gtcggctttt ctgctcgcgt atgtcgtact gaaaaagcgg atcgcctggc
     1561 gaaagatcca gccccggttt ccggccgcca aagattaccg ccgcgagatc atagattcca
     1621 ccatctccat ccttattttc gcaatgatgc cgatcgttat cctgcgggtg ccggcgataa
     1681 ggatgcatac tcaattgtat gggccaccgc cggagcgcgg ctggctgtat ttcttcctgg
     1741 tgttcccaat catgttcgtg atccatgatg cctatttcta ctggatgcac cggctcatcc
     1801 atcagccgaa actgttccgg ctgatacacc tcgagcatca ccgctcggta aatccatcgc
     1861 catgggcggc ttatgcgttc ggaccgatcg aggcgttcct ggaagcgctg atttttccga
     1921 tcatcttatt tacgatcccc gtaacggtct ggcatgtttt tattttcttt ttgttaagca
     1981 ttgtgtacaa tgtatatggg cacctggggt ttgagctgta tccgaagggt tttcaccgga
     2041 cgctcctggg ccggtggata aacacctccg tttcccacaa tcagcatcac cattatttca
     2101 aggggaatta cggcttgtat ttcctgtggt gggacaggtg gatgggcact atccggaagg
     2161 actatgacca ggctttcggg gaggtgaccg ggcgaataaa gcatgcggcg cggacgaccg
     2221 gttaagttgt caggagttct atgccgcttt cagtaatgcg gatctttctc tccttataga
     2281 gctttcctgt tgtcattttg aaggtttttt tgctcatacc gaagaacgaa tagatcgccc
     2341 ccgggtccga tctgtcatgg taggggagcc gtcctttatt ctcttccagc aggcgaagca
     2401 ccttggctgc ggcgtcttcg acgcgggcgt acccgggccg tcctgccgcg acgtcgatct
     2461 gtgtctcgcc cgagggggat tggtttgcgg gcaggacatt cttgatgaat cccggaaagc
     2521 ggtccccgac tttaatgtcg cggtacacct cgttataatg aaggacaccg gtgtggcggt
     2581 tgttgatggc acaaagatag ccgatgtccg tgcggcggat gacggtgagc gtgacaatgt
     2641 cgttgtcggt taccgttagg ttattgttgg tcaggaaagg ctcgaatttt tcggtcgctg
     2701 cgaggcggcc ggtctgctca tcgaggtaga tcttgacgag ataatcgccg ccgaggcgca
     2761 ttccggtgag ttgtttggct ttcgggacga acaggtcctt catcagcccc cagtcaagga
     2821 acgcgccctg cggggtcagg ctgacgacgc gaagcaggac gatatccccg agaatccctt
     2881 ttgggtgttg cgtagtggct atcagccggt cctcgctgtc gtgatacacg aagacacgca
     2941 gcaggtcgcc gggtttggca tttcgcgggg cgaatcgctt gggtagaagg atgccctcag
     3001 cgccgtcatc gaggatgagg cccatattca gctggcgttt gacgaccaaa ttattaaata
     3061 ctcctacttg gaccattgtg caaaaatagg ggaaaccgaa atcggggcga ggaaaacttg
     3121 cctggatggc cggatgcaga agggccgaaa aacttctgta gattcgccgg atgagaagat
     3181 caatcatcct tagctgcgtg ttctttgcgg cctgccacgg gacgaacaat ccaatcctcc
     3241 ggccgggaag cagcttcgcc aataatagcg gcagtccaaa atgggtctcc ctgcctgaca
     3301 gcagccggat aaagatcagc gacggaacga ccatcgtggt tggagccggg tattcgaggg
     3361 gcaaccgcgc ggttgacctt gacggagagg gcatgtttat gatccggggc ggggaccggt
     3421 ttgtggtgac gacaaagaac ctgtacatcc agggcatggg cacgaaattc agggtggatg
     3481 cgccgcgcaa taaaccgggc gaagaagtgg acctcctgga tggagtgatg gtgatccgga
     3541 agatgtacca ttccgacctg gatagcgcgc aagaagtgct ccatgcgggg gatatgctga
     3601 tgatcaaccg cgagatcgac ctcatggaaa aggaaaagat ggacacggat gaacttcata
     3661 aggccgaggc aaagttttga aagcattccg gcagcgaact attttatttg ttctgggtta
     3721 agacgggtat gccaagggtc gtatcatacc agatagccga caatatcgat gtcaggggcc
     3781 tccggacggc ctacaaagaa ggcatatacc tgtatgatgc cgacgagctt ttctacaaga
     3841 tgggcgaggg acgttatgtg tatgtgttta aatatggtgt ggtctgtttc atgggatata
     3901 acgaagggga ggcagggctt tatttggaac aggtagggcc gttctgtaaa aatttgttcg
     3961 agcgccgcct gagcgaggag tttgaagtgg agaccaatgc gcaggagaat aagtttggtt
     4021 ataacaagct ggagatcacg aagccggata tcgaggttat tcggctgatc atgctgaatg
     4081 tgagtcagtc ggtcgcgctg gattactttt ccgagcagac caatctgttg caggaagaaa
     4141 cgaactccca caccctcaac ctcgagcggc ggggcaggtt gaagatctcc ggggccgccc
     4201 tgaaaaaata tatcggcaag acgctgcatc tgaaaaaccg gatttccgag aacctgtaca
     4261 tcttcgattc tccgccgcag acctgggagg atgagaacct aaaccggatc gatcagggcc
     4321 tgaagcggac ctttgatctt caggagcgtt accgcgatat ccgggaaggc cttgagatcg
     4381 taagggaaaa tctcgagcta ttcaaagacc tcctgcaata ccgcaatagc actttcctcg
     4441 aatggatcgt tatcgtcctc gtggccgctg aggtggtcaa tctattactt gacaagttat
     4501 ttaagctgaa gtagtgtccg gccggtggga tgggaggcaa agcaggccgg gtgctaaaaa
     4561 aaagaagccc agattgaaat ccaggccgtt gctaaaatcc aatatcctat taaaagcctt
     4621 agtaatactg caaacaccga tccaggtggt tttcacgcac aaaagcggcc ggcagcgcgg
     4681 tattccgagg aaatgtgtgg agctttttcc tcattcgtcc caaattttcc ttagcggcgg
     4741 tagtgccacg gccgtaactt tggcggatga gcagggaaac cgacaagatg tttttgggtc
     4801 aggctgtcgc cctggccagg gaaggcatcc tcgccgggcg gggaggccct ttcggctgcg
     4861 taatcgtccg cagcggcgag gtgatcggcc ggggaagtaa cagcgtaacg tcgacgaacg
     4921 acccgacggc acatgcagaa atcgtggcaa tccgcgaagc atgcgtgcga ctcggagatt
     4981 atcaattggc aggctgtacc ctgtacgctt cc
//
LOCUS       DATGKE010000062         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1124739, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000062 DATGKE010000000
VERSION     DATGKE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124739"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..597)
                     /locus_tag="VKU83_01040"
     CDS             complement(190..597)
                     /locus_tag="VKU83_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HLI92156.1"
                     /translation="MNRKKDISGGDDIRLLVDGFYEKARKDPALGPIFNDIARVDWEH
                     HLPKMYAFWGNVLLQKGDYSGNPMATHLELNALTPLKKAHFDRWLQLFRETIDENFEG
                     EVAERARQRSLSIATIMQMKIIGDRNSPSVKGI"
     gene            complement(661..1236)
                     /locus_tag="VKU83_01045"
     CDS             complement(661..1236)
                     /locus_tag="VKU83_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HLI92157.1"
                     /translation="MEPGYDTTHTALRCWWLFLFSGILLFIAGCGVLRYPVGTYLTIS
                     LLLGIVVVAVGVAHIYFAVANSKILPRRPWHLIIGIVDLIIGVLLFSYPDITMAVLPP
                     LVGIWFLVRGASLLVYAYTLRRLHGRSWGWFLAGGILIAIFAGFIIYYPVFEFFTIVV
                     WTAAALMITGISNMLLAFRLKAKKDETAIIL"
     gene            1310..>3405
                     /locus_tag="VKU83_01050"
     CDS             1310..>3405
                     /locus_tag="VKU83_01050"
                     /inference="COORDINATES: protein motif:HMM:NF039503.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5695 domain-containing protein"
                     /protein_id="HLI92158.1"
                     /translation="MRLSVFCLLALAALSGWKLDAQSPWARLAKRPSTLGLEGGFLEL
                     RTPAYRLRLVRSSQTVAFLGPAGGSFDFTPGDSLHIRSSDGMYHLGDITLRIKNDSGG
                     WTTYSSAARRRPVEPLPAGGNILAAADLAATFPDGIPLRIRRYWELDKGELTLRFALT
                     NNSPNPVEIGSLGIPMIFNNLLDGKTLEQAHAENVLYDPYTGRDAGYLQVTRLSGEAP
                     SLIVVPYGNTAFEAYNPLLNDPTPRGITFEGFYEWLVCSKAYAENEWKAAQPWNDPTN
                     IVLKPRETRSFGVQFLLSGTARHIEQTLIAHKRPVAVGIPGYVVPMDVDARLFLRYPR
                     EVRKMEVEPAGALEIAPSAEVGVVTRADGEKEWKGFAVHGRIWGRARLTVTYADGVVQ
                     TIQYKVIEPEKKVVASYGHFLTTEQWFDNPTDIFHRSPGVITYDEDKKAQVVEDNRAW
                     IAGMSDEGGAGSWLGALMKQLVLPDKAELEKLEAFADQTLWGHIQYDSGAHSYGVRKS
                     LFYYAPDSLPQGTYDTSINFRTWSAWPRKEAESTGRSYDYPHVAAAWWVLYRLARYHS
                     GLVSTHRWDWYLEHARRTILAMVEQAPYYSQFGQMEGTVFYLILRDLRLEGLTDEADA
                     VEAAMKKRALHWRSLQYPFGSEMPWDSTGQEEVYIWSLFFGFSGKADVTLKAILAYMP
                     TLPSWAYNGNARRYWD"
BASE COUNT          767 a          874 c         1050 g          714 t
ORIGIN      
        1 tatccggcaa atgcgctcgc cgagggtaag gaggatatat tgagcgggcg tgacaattag
       61 accaagaccg cggccgggcg cgtccttctg caggagagtc atagcgaaag attttagtgg
      121 tggtcagcca ggcatctaaa attccgccat gccgattaaa attttattag aatggaatta
      181 gaaatgctgt taaattcctt taacagacgg agaattccgg tcgccgatga tcttcatctg
      241 cataatggtt gcaatggaca gcgagcgctg acgcgctctt tccgctacct caccttcgaa
      301 gttttcgtcg atcgtttcgc ggaacagctg gagccagcga tcgaagtggg ctttcttgag
      361 cggcgtgagc gcgtttaatt ccaggtgtgt ggccatgggg ttgccactgt agtcgccctt
      421 ctgaagcagc acgttccccc agaacgcata cattttaggc agatggtgct cccagtccac
      481 gcgggctata tcgttgaaaa tagggccaag ggctggatcc ttacgggcct tttcatagaa
      541 gccatcgacc agcaaacgga tgtcgtcgcc gccactaata tccttcttcc tgttcataac
      601 ggcgcaaata taggcaaaaa aagggctccg gtcagtgcag ggggcaccat cgcttccggc
      661 tcagaggatg atcgcggtct cgtccttttt ggcttttagc cggaaggcca ggagcatgtt
      721 ggatattccc gtgatcatca gggcggcagc cgtccatacg actatcgtga agaattcgaa
      781 tacgggataa tagatgatga agccggcaaa gatggcgatg aggatgccgc cggccaggaa
      841 ccatccccag ctccggccat gcagccgtct aagcgtgtaa gcatagacca gcagcgaggc
      901 gccccggaca aggaaccaga tgcctaccag cgggggcagg accgccatgg tgatatccgg
      961 gtaggagaag aggagaaccc caatgatcag atccacgata ccgattatga gatgccaggg
     1021 ccggcggggc aggatcttac tattggccac cgcgaaatag atatgggcga cgcccactgc
     1081 aaccacaaca atacccaaca acagggagat ggtgagatag gtgcctaccg gatagcgcag
     1141 caccccgcac cccgcgatga agagcaggat cccgctaaac aggaacaacc accaacatcg
     1201 gagagccgtg tgggttgtat cgtagccggg ttccattagt taaaatttgt gtattgcaag
     1261 ataagaattg tgaagggcaa tcagcggcaa aacttatttt agcgtctgta tgcggttaag
     1321 cgttttttgt ctgctggcgc tggctgccct ctcagggtgg aagctggacg cgcaatcgcc
     1381 ctgggcgcgg ctggcgaagc gtccgtcgac gcttgggttg gaaggaggct ttctggagct
     1441 gcgcacgccc gcttatcggc tccggctggt acggtcgtcg cagacggtgg cctttctggg
     1501 accggctggt ggaagttttg attttacgcc gggagatagt ttgcacatcc ggagcagcga
     1561 cgggatgtat cacctgggtg acatcaccct gcggatcaag aacgactcgg gtggttggac
     1621 gacctattcc tctgctgccc ggcgcaggcc tgtggagccg ctgcctgccg gcggaaacat
     1681 cctggcggcg gctgatcttg ctgctacttt ccctgatggc atccccctcc gcatccggcg
     1741 ctattgggaa ctggataagg gagaactcac gttacgattt gccctcacca acaattcgcc
     1801 gaatccggtg gagatcggtt cgctggggat accgatgatc ttcaacaacc tcctcgatgg
     1861 aaagacgctc gagcaggcgc atgccgaaaa tgtgctgtac gatccctaca ccggtaggga
     1921 tgccgggtat cttcaggtga cgcggttgag cggagaagcg ccttcgctga tcgtcgtgcc
     1981 ttatgggaat acggcattcg aagcctacaa tcccctgctg aacgatccaa cgccacgagg
     2041 gatcacgttc gaaggttttt acgagtggct ggtgtgcagc aaggcctacg cggaaaatga
     2101 atggaaagcg gcacagccat ggaatgaccc taccaacatc gtgttgaaac cgcgggagac
     2161 gcgttctttt ggggtacagt tcctgcttag cggaacggcc cgccatatcg agcagacatt
     2221 gatcgctcat aagcgccccg ttgccgtggg gattccggga tatgtggtgc ccatggatgt
     2281 ggatgcgcgg ctgttcttgc ggtatccgcg ggaagtgcgg aaaatggagg tggaacccgc
     2341 gggtgcgctg gagatcgcgc cgtcggccga ggttggggtg gtgactaggg ctgacgggga
     2401 gaaggaatgg aaaggcttcg cggtgcatgg caggatatgg ggaagggcga ggctgaccgt
     2461 cacctatgcc gacggggtcg tgcaaacgat ccaatataaa gtgatcgaac cggaaaaaaa
     2521 ggtcgttgct tcctatggcc attttctgac aaccgaacag tggttcgata acccaaccga
     2581 tatctttcac cggagcccgg gagtgatcac atacgacgaa gacaagaagg cgcaggtcgt
     2641 ggaggacaac cgcgcctgga tcgccggtat gagcgatgag ggcggcgccg gaagctggct
     2701 gggcgccctg atgaaacagc tggtgttgcc cgacaaagcg gaattggaaa agctggaagc
     2761 cttcgcggat cagacgttgt ggggccatat ccagtacgac agcggcgccc atagctatgg
     2821 cgtacggaaa agtcttttct attacgcgcc ggattcgctg cctcagggaa catacgacac
     2881 ttccataaat ttccgcacgt ggtcggcctg gccgcggaag gaagcggaat cgacgggccg
     2941 ttcgtatgat tatccgcatg tcgcggcggc ctggtgggtg ctatatcggc tggcgcggta
     3001 ccattccgga ttagtttcga cgcatcgctg ggactggtat ctggaacatg cgcggcggac
     3061 gatcctggcg atggtagagc aggcgcccta ttattctcag ttcgggcaaa tggagggaac
     3121 ggtgttctat ctgatcctgc gcgatctgcg gctcgaaggg ttgaccgatg aggcagatgc
     3181 cgtagaggcc gccatgaaaa agcgtgccct tcactggcga agtcttcaat atccattcgg
     3241 cagcgagatg ccatgggact cgacgggaca agaagaagta tacatctggt ccttgttctt
     3301 cggcttttcc gggaaggccg atgtgaccct gaaagccatt ttggcctaca tgccgaccct
     3361 cccgagctgg gcatataatg gcaatgcccg ccgctactgg gattt
//
LOCUS       DATGKE010000063         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_293675, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000063 DATGKE010000000
VERSION     DATGKE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293675"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VKU83_01055"
     CDS             <1..305
                     /locus_tag="VKU83_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI92159.1"
                     /translation="PSYREGMPMSLLEGASMCKALIAGDGPGCRTLVRDGVTGFLCRT
                     RDSADLAAKMLAYFHLPAQAKKQMGMAARERLMSGFTREQILQVYLDKINALPASG"
     gene            317..1561
                     /locus_tag="VKU83_01060"
     CDS             317..1561
                     /locus_tag="VKU83_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92160.1"
                     /translation="MHIALFILYSALCGYGILKLPFFRRTGIRPGILLAFFAIHVGAG
                     CLHNLIAWRYYPDHGDIWDSFREGIQLRNWLQTDFQQFLASNSRWTDFTHNGVKFIHL
                     ILNLLSMDNLYINTLLFAFPVFLGNTALYFVFRRHFQAPLTPLVVFLLPSTLFWTACV
                     FREGVLYMLLGFFFYQLDRVFSSPAPAAVSHNRQAAAARTPAAIAAFCCFLLILYFRF
                     TVGIVLAPALLVMRWVNKPSTKEILAAAGAALTVLVIMLAVPPFPRWLLQGLRQWQAE
                     FQILEGHSRLYLPAMDGTWPGLFRIMPYALLNGWFQPLPGAGGRPIYLAFSIELILIW
                     AVVALALVRAATSPATPALPAARRRDGFSAACILFALLGMFGIGLIVPFAGAIVRYRS
                     IYLPFLLLPALHSLRDWRRFRA"
     gene            1784..>3555
                     /locus_tag="VKU83_01065"
     CDS             1784..>3555
                     /locus_tag="VKU83_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HLI92161.1"
                     /translation="MSSLRFKAIDNLNTTTETIIPGAAKITAIFGENVFTLRTAREFL
                     SDEAYKSLLASMKSGKKIDRAVANQIASGIRQWAEGKGVTHFTHWFQPLTGSTAEKHD
                     SFFTIKSDGTAIEEFDGAALIQQEPDASSFPSGGLRATFEARGYTAWDPSSPAFVMEI
                     GSGKTLCIPTIFVSYTGESLDYKAPLLKALAALDKSAVEVCHYFDKNVTKVTATLGWE
                     QEYFVVDEGLFNARPDLVLSGRTVFGHAPAKGQQLEDHYFGSIPERIYAFMRDYEQEA
                     YKLGIPLRTRHNEVAPAQFECAPIFEEVSVAVDHNILLMDIMDRVARRHKLRVLLHEK
                     PFAGINGSGKHNNWSMATDTGINLLAPGKTPKTNLMFLTFFVNTIKAVHDYADVLRAS
                     IASAGNDFRLGANEAPPAIISVFIGEYLTKVLADVKERVGDKFDEQDEAILKLDLHRS
                     IPELLLDNTDRNRTSPFAFTGNKFEFRAVGSGANCANAMIVLNTILAETLKRFKKDVD
                     TLIEKGDKKEIAIMHVIREYVVKSEKVLFEGDGYSEEWHHEAERRGLPNVRTTPLALD
                     AMVTDKAKQLFETNGVYTHVELEAR"
BASE COUNT          731 a         1083 c          924 g          817 t
ORIGIN      
        1 tgccatccta tcgggagggg atgccgatga gccttctgga aggcgcgagc atgtgtaagg
       61 ccctgatcgc aggcgatgga cccggatgcc ggaccctcgt tcgggatggc gtcactggtt
      121 ttctttgccg gacgagggac agcgccgacc tcgcggcaaa aatgctggct tactttcacc
      181 tgccggccca ggcgaaaaag cagatgggaa tggctgcccg cgagcgactg atgtccggat
      241 ttacgcgcga gcagatcctg caggtttacc tggacaaaat aaatgccttg ccagcctccg
      301 gctaaatccg ccatccatgc acattgcatt attcatactg tatagtgccc tttgcggcta
      361 tggcatcctg aagctgccgt tcttccggcg caccggcatc cgcccgggca tcctgctggc
      421 ctttttcgcg atccatgtcg gagccggctg cctgcataac ctgatcgcct ggagatacta
      481 tccggaccac ggcgatatct gggacagttt ccgggagggg atccagctcc ggaattggtt
      541 gcagaccgat tttcagcaat tcctggcatc caactccagg tggacggact ttacgcataa
      601 cggggtcaag tttattcacc tgatattaaa tctgttgtcc atggacaacc tatatatcaa
      661 tacactgctt ttcgcctttc ccgtcttcct tggcaacacc gccttatatt tcgttttccg
      721 ccgccatttt caagctccgc tgaccccgct cgtcgtcttt ctgttgcctt ccaccctctt
      781 ttggacggcc tgtgtcttca gggaaggcgt gctgtatatg ttactcggct ttttctttta
      841 ccagctcgac cgcgtgttct cttctcccgc gcccgccgcg gtttcccaca accgtcaggc
      901 cgccgccgca cgaaccccgg cagcaatcgc agccttttgt tgcttcctgc tcatactcta
      961 ttttcgcttc actgtcggca tcgtcctggc tcccgcatta ctcgtcatgc gctgggtcaa
     1021 taagccgtcc accaaagaga tcctggcagc cgccggcgca gcgctgaccg tccttgtgat
     1081 catgctggcc gttcccccgt ttccccgctg gctgctgcag ggtctccgtc agtggcaggc
     1141 cgaatttcaa atccttgaag gccattcccg gttatacctt cccgcaatgg acggcacctg
     1201 gccaggcctg ttccggatca tgccctatgc gttgttgaat ggatggttcc aaccattgcc
     1261 gggagccggc ggccgcccca tctaccttgc tttttcgatt gaactgatcc tgatctgggc
     1321 ggtcgtggcc cttgcccttg tccgtgccgc cacgtccccc gccactccgg cccttccagc
     1381 tgcacgccgt cgcgatggtt tctccgcagc gtgcatcctt ttcgcgctcc tggggatgtt
     1441 tgggatcgga cttattgtcc cattcgccgg tgcaatcgtc cggtaccgga gcatttacct
     1501 tccattcctg ctcctcccgg cccttcattc gttgcgcgat tggcgccggt tcagggcctg
     1561 aagcccttgc ccggggttgt ccggggttgt ccgggggctg gccggcagac atattataat
     1621 ttttaacata actaaaaaac ttatcgctgg cctgagcaaa atgcctgatt tatagccatt
     1681 acaccatttt ttcaccgctt tttttcgggt ttcgcacccc cagtttgggc aaagaattgt
     1741 ttaatatccg gcaatccttt agtttcgcct aaattttatc agcatgtcat cgctacgatt
     1801 taaagctatc gataacctaa acacgacaac cgagacgatc ataccgggtg ccgccaagat
     1861 taccgccatt tttggtgaga atgtcttcac attaagaacc gcccgtgaat tcctgagtga
     1921 cgaggcatac aagagcttgc tggcttccat gaagagcggc aaaaagatcg atcgcgctgt
     1981 cgccaaccag attgcctccg gtatccgtca gtgggcagaa gggaaggggg tgacgcactt
     2041 tacgcactgg ttccagccgc tgactggaag caccgcagaa aaacacgatt cattcttcac
     2101 cataaagagc gacgggacgg ctatcgagga gttcgacgga gccgcactga tacagcagga
     2161 accggacgcg tcctcctttc caagcggagg tctgcgagct acgttcgagg cccgcggcta
     2221 taccgcctgg gatccctcgt cgccggcctt cgtcatggag atcggctcgg gcaaaacgct
     2281 ttgcatcccc accatcttcg taagctacac cggcgaatcg ctggactaca aggcgcccct
     2341 gttgaaggca ttggccgccc tggacaagtc cgccgtggag gtttgccatt acttcgataa
     2401 gaatgttacg aaggtgaccg caacgctcgg ctgggaacaa gagtatttcg tcgtcgacga
     2461 aggtctcttc aatgcccgcc ccgaccttgt tctgagcggt cgcaccgtat tcggccatgc
     2521 cccagccaag ggccagcagc ttgaagacca ttatttcggc agcattcccg agcgcatcta
     2581 tgcctttatg cgggattacg aacaggaggc ctacaaactc ggtataccgc ttcgcacccg
     2641 ccacaatgag gtcgctccgg cacagtttga gtgcgccccc atcttcgagg aggttagcgt
     2701 cgccgtcgac cacaatatcc tgctgatgga catcatggat cgcgtcgctc gccggcataa
     2761 gctgcgcgtc cttctgcacg aaaaaccttt cgccggcatc aacggcagcg gtaaacacaa
     2821 caactggagc atggccacgg acaccgggat caacctgctt gcgccgggaa aaacaccgaa
     2881 gaccaacctg atgttcctca cgttcttcgt caacaccatc aaggcggtcc atgactatgc
     2941 cgacgtactc cgcgcttcca ttgcctcggc cggtaatgac ttccgcctgg gggccaacga
     3001 ggcgccgccc gccatcatct ccgtctttat cggggaatat cttaccaagg tacttgcgga
     3061 tgtcaaggag cgggtgggcg acaagttcga cgaacaggac gaagcgatcc tgaaactgga
     3121 ccttcaccgc agcatccctg aactcctgct ggataatacg gaccggaacc gcacttcacc
     3181 cttcgctttt accgggaata agtttgagtt ccgcgccgtc ggttccggcg ccaactgcgc
     3241 caacgccatg attgtcctga ataccatcct ggccgaaacc ctgaaaagat tcaagaagga
     3301 tgtcgacacg ctgatcgaga aaggtgataa aaaggagatc gcgatcatgc atgtgatccg
     3361 cgaatacgtc gtgaagagcg aaaaggtact gttcgaagga gacggctata gcgaagaatg
     3421 gcaccatgaa gctgaaagac gcggcctgcc taacgtccgg accaccccgc tggcgctgga
     3481 tgccatggtc acggacaaag cgaaacagct cttcgagacg aatggggtct acacccatgt
     3541 cgaactcgaa gcccg
//
LOCUS       DATGKE010000064         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1222368, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000064 DATGKE010000000
VERSION     DATGKE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222368"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VKU83_01070"
     CDS             <1..1266
                     /locus_tag="VKU83_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLI92162.1"
                     /translation="LAEITGFAATSLQPNSGAQGEYAGLLTIRAYHAAHGGAHRDVML
                     IPISAHGTNPASAVMAGMKVVVVKSDEDGHIDVADLNSKAIQYREQLAGLMVTYPSTH
                     GVFEESISEICRIIHENGGLVYMDGANMNAQVGLTSPGLIGADVCHLNLHKTFAIPHG
                     GGGPGMGPICVNEKLKPFLPSHVNLSGYEVPFSGNGHHVPDLRGHAVSAAPFGSASIL
                     LISYAYVKMLGAEGLTRATKYAILNANYMRARLEKYYKVLYTGAGGTCAHEFIVDLRP
                     FKASAGIEAEDVAKRLMDYGFHAPTLSFPVPGTIMIEPTESEDKAELDRFCDAMISIH
                     EEIRAIGEGRADSSDNVLKNAPHTLHVICSDDWKHPYSRSQAAFPLPYVRGSKFWPSV
                     ARVNNTYGDRNLICTCEPVSSYAEEPVSN"
     gene            complement(1339..2058)
                     /locus_tag="VKU83_01075"
     CDS             complement(1339..2058)
                     /locus_tag="VKU83_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HLI92163.1"
                     /translation="MSKTRKQFFWTSIILGMAAGMTGCVKGGSPVTVSPISYVSVMNE
                     APYGVPVDIYFNGTLVSPAGGIAPGQYSSAYGSLKPGNYTVEFKKTGTDSVVYQLPAS
                     NYDTSAFYTLILYNVNADSTAVQAAKIQDDFSQVTASSAYYRFFNLSPDVPGANLSLN
                     DQVAQNNRTPADNVLTSAAYDAFMPLAGGTYTIHVENAATDSVLASLPQTALHSGDVY
                     TIFLAGTLKSGLSVNVLQAVY"
     gene            complement(2224..>2549)
                     /locus_tag="VKU83_01080"
     CDS             complement(2224..>2549)
                     /locus_tag="VKU83_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HLI92164.1"
                     /translation="FFKNLKNGHTVKSPFKVEMGVDGLKLDTAGAVVAGTGHHHLLID
                     AGDSIPAGVVVPKDSTHLHFGKGQSSTELKLPAGEHTLTLQFADGAHRSYGSQLAATI
                     KVKVK"
BASE COUNT          543 a          747 c          715 g          544 t
ORIGIN      
        1 ctcgctgaga tcaccggctt cgccgcaacc tcgcttcaac cgaacagcgg cgcgcagggc
       61 gaatatgccg ggctgctgac gatccgggct tatcacgccg cccatggcgg cgcccaccgc
      121 gacgtgatgc tgatccccat atccgcccac gggacgaatc cggccagcgc cgtcatggcg
      181 gggatgaaag tggtggttgt gaagagcgat gaagatggcc acatcgacgt cgccgacctc
      241 aacagcaagg ccattcaata ccgggagcag cttgccggcc tgatggtaac ctacccctcg
      301 acacacggag tatttgaaga aagtatttcc gagatctgcc gcattattca tgaaaatggc
      361 ggcctggtat acatggacgg cgcgaacatg aatgcccagg tcggcctcac cagcccgggg
      421 ctgatcgggg cggacgtctg ccatttgaac ctgcacaaga ctttcgccat tccccacggc
      481 ggcggcggcc ccggcatggg tccgatctgc gtcaacgaga aactgaaacc gttcctgcct
      541 tcccacgtca acctttccgg ttacgaagtg cccttttccg gcaacggcca tcacgtcccc
      601 gatctgcgcg ggcatgccgt ctcggcggcg ccattcggat cggccagcat cctgctgatc
      661 agttatgcct atgtcaagat gcttggtgcg gaaggcctca cgcgggccac aaagtacgcc
      721 atcctcaacg ccaactatat gcgggcccgg ctggaaaaat attataaggt gctttacacc
      781 ggcgcgggcg gcacgtgcgc ccacgaattc atcgtggatc ttcgcccttt caaggcaagt
      841 gccggcatcg aagccgagga cgttgcgaaa cgcctgatgg actatggctt ccacgcccct
      901 accctgagct tccccgtacc gggtacgatc atgatcgaac cgaccgagag cgaagacaag
      961 gcagagctgg accgcttttg cgatgcgatg atttcgattc acgaggagat ccgcgccatc
     1021 ggggaaggca gagccgacag ttcggacaat gtgctgaaaa atgctccgca taccctgcat
     1081 gtcatctgtt ccgacgactg gaaacatccg tattcccgca gccaggccgc atttccgctg
     1141 ccgtatgtcc gcgggagcaa gttctggccc agcgttgcca gggtcaacaa tacttatggc
     1201 gaccgcaacc tgatttgtac ctgcgagcca gtcagctcct atgcggagga gccggtttca
     1261 aattgagtca tccaatgcta ttgggaaagg ccgctccaag gcaagggggc ggccttttct
     1321 ctgtttcctg tgttgcagtc aatagaccgc ctgaagcacg ttcacgctta gccccgactt
     1381 gagcgtcccg gcaaggaata tggtatagac gtcgcccgaa tggagcgccg tctgcggcaa
     1441 gctcgccagg acgctatccg tcgccgcgtt ttccacatga atggtatatg tcccccctgc
     1501 cagcggcatg aatgcgtcgt aagccgcgct ggtcaggaca ttgtccgcag gggtccggtt
     1561 gttctgcgcg acctgatcgt tgagggacag gttcgcaccc ggtacgtccg ggctcaggtt
     1621 aaaaaagcgg taataggcag atgaagccgt gacctgcgaa aagtcatcct ggatctttgc
     1681 ggcctgcacc gccgtcgagt cggcattcac attataaagg atcagggtgt agaacgccga
     1741 cgtgtcatag ttcgacgcag gaagttggta cactacactg tccgttcccg tctttttaaa
     1801 ttcgaccgtg tagttccccg gcttcagtga accatatgcg gaagaatact gccccggggc
     1861 gatgccgcct gcagggctta ccagggtccc gttgaagtag atgtcgacgg gcactccata
     1921 cggcgcctcg ttcatgaccg aaacatagga aatggggcta acggtgaccg gagagcctcc
     1981 tttcacacac cctgtcatgc cggccgccat cccgagaata attgaagtcc aaaaaaattg
     2041 cttgcgggtc ttactcatgt cgtcattcgg attttattct gtaaatatta agatacgatc
     2101 aatttttcca cctgacaact attacggggt tttcgttgcg cgggttgtct ccggctccgc
     2161 tggaaaaaac aagcgcttgc cgccggctaa ccggacggca agcgccagga tgcagtcaat
     2221 atcttatttc actttgacct tgatcgtcgc cgccaattgg ctgccatagg accggtgagc
     2281 tccgtcggca aactggagcg tgagggtgtg ctcgcctgcc ggcagtttga gctccgtact
     2341 gctttgcccc tttccgaagt gcaggtgggt ggaatccttt ggaaccacca ctccggcggg
     2401 tatggagtca cccgcgtcga tcagcaggtg atgatgaccg gtacccgcaa cgacagcgcc
     2461 cgcggtatcg agtttcaatc catcgacacc catttccacc ttgaaaggtg atttgacggt
     2521 atggccgttc tttaggtttt tgaaaaaga
//
LOCUS       DATGKE010000065         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1222040, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000065 DATGKE010000000
VERSION     DATGKE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222040"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..2758)
                     /locus_tag="VKU83_01085"
     CDS             complement(83..2758)
                     /locus_tag="VKU83_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLI92165.1"
                     /translation="MSSATINCPEPQNAQPSGPADNYAALLRPFELNFKATGYHFQVG
                     ETQATQGWIIHLSAVLSQTVPLIEAIIPYLIDKDLAFKIPLGHAIAEDILNGTLGITE
                     IGKIVTIYPEPALQTIQVAKDLLSLTKSFKGPAVPTDSLLGGIVYTRYGSFRPIMKLG
                     PDGIRRKYFYDKAGLLTEDCQTIPFRLPAGTTWPFDEISRVNIAEPPKPPGHLFKITG
                     ILKQDPRGNVYKALYLKNLLNVKKCVIKQGISNMASDPEGRDIQDRLRWQSELHQRLC
                     NRISLPKPYHLHSAQGAIMFIMEYIPGCSLFEKLLAINPSSGTWTELSGTQRAKILKY
                     AIQIAKSINILRESGYIHRDINPVNFLVDKKDRIVLIDLELAYSIRDDRPSPPFRQGT
                     PGFMSPQQARFSQPTLYDDIYSLGATLLFMFTGLLPVQCDTKSANQLAASLYALIGNQ
                     SLAAIISDCFTTEGQDRPAINTIIQTLETCYRETQTARRPGIRAAGGKLSVSELDEVI
                     ASAIQGLTQQPIPILHNLWLSKQLTAENLPAPQIKRYAANPGTAEGVSGVVYALARIS
                     KAGTCIDSCRDQYAEGWRFLLEACSDDCRPLPGGLYNGKAGIAMSLAEGISSGLVSGT
                     AALTNLTGKLLDSKPEGFSLATGVAGQGVSILSCTRLLKTDAFDHLLEKRVDDLLGEQ
                     RKDGSWFAVPDATRSHGEWMDAAFDQTGPGLFLTKYLAFRPNPRVEKALTHAISRKVR
                     NKSFLKHFYHTLASKAGYNIGDGGKGFLLLLIKAYELFNDKVYKEIATAALATLPRFL
                     LHPNFTTQNGLAAMGELYLEAFRVFKTAEWKTRADWIANVFCHTIIRNPDGSGYWVLE
                     QNNPPTADLLTSITGIIHFLSRCAHPAEPGYRIID"
BASE COUNT          606 a          689 c          799 g          724 t
ORIGIN      
        1 actttttcat aaaaaatgtt tagtgttcta aattttgcct actctactag gttttcggct
       61 tcccctttta ttgcgcaatg cattaatcga tgattcggta gcccggctcc gctggatgcg
      121 cacagcgact caaaaaatga atgatccctg tgatgcttgt tagcagatcg gcggtaggcg
      181 ggttattttg ttccagcacc cagtagccgg agccatcggg gtttctaatg atcgtgtggc
      241 agaaaacatt tgcaatccaa tcggcgcgcg ttttccactc cgccgtttta aagacgcgga
      301 aggcctcgag atagagttcg cccatcgccg ccagcccgtt ttgcgtcgta aagtttggat
      361 gtaataagaa gcgtggcaat gtggccaagg ctgcagtggc gatttcttta taaaccttat
      421 cattgaagag ttcataggcc ttaataagaa gcagcaggaa gcctttcccg ccatcgccaa
      481 tattgtagcc ggctttggaa gccagggtat ggtaaaaatg tttcaggaag gatttatttc
      541 ggactttccg cgagatggcg tgggttaagg ctttttcgac ccgcggattt ggccggaacg
      601 ctaagtactt agtgagaaag aggccagggc cggtctggtc aaaggcggcg tccatccatt
      661 cgccatggga tcgggttgca tcggggaccg cgaaccaaga cccatctttt cgctgttcgc
      721 ccaggagatc atcgacccgt ttttcaagta aatggtcaaa ggcatccgtc tttaacaggc
      781 gcgtgcagga taagatcgat accccctggc cggcaacccc ggtcgcgagc gagaaaccct
      841 cgggcttgga atctaagagt ttaccggtta ggttggtgag ggccgctgtg cctgatacca
      901 agccgctact tattccttcg gctaaagaca tcgcgatgcc ggcctttcca ttgtaaaggc
      961 cgcctggcaa cggccggcag tcatcgctgc acgcctccag caggaatctc cacccctcgg
     1021 catactggtc ccggcaggaa tcaatgcagg ttcccgcttt cgagatccgc gccagcgcat
     1081 atactacgcc tgatactccc tcggctgtcc ccggatttgc cgcgtaccgt tttatttgcg
     1141 gcgccgggag attttcagcg gtaagctgtt tcgaaagcca aaggttatgt agtatcggga
     1201 tcggctgctg ggtcagtccc tgaatggcgg aggcgattac ctcatctaat tcggagacgc
     1261 ttagcttgcc gcccgcagca cggattcccg gccgccgggc ggtttgtgtt tcccgatagc
     1321 atgtctccaa ggtttgaata atggtattaa tggccggcct gtcttgccct tcagtagtaa
     1381 agcaatcgga aatgatcgct gccaggcttt ggttaccgat caaggcgtac aagctggcgg
     1441 cgagttggtt ggcagatttc gtgtcgcatt ggaccggcag gaggccggta aacataaaaa
     1501 gcagggttgc gccaaggctg tagatatcgt catagagggt tggctgagaa aacctggcct
     1561 gttgcggcga cataaagccg ggagtccctt gcctgaatgg tgggcttggc cggtcgtccc
     1621 ttatcgaata ggccagctcc aggtcgataa ggacaattct gtccttttta tcaaccagga
     1681 agttaaccgg gttgatgtcc cggtgaatat atccggattc gcgcaggatg ttaatggatt
     1741 tggcgatttg aatggcgtat ttcaggattt tggcccgttg cgtcccggat agttcggtcc
     1801 aggttcctga agatggattg attgccagta gtttttcaaa aagcgagcat ccagggatat
     1861 actccatgat aaacattatg gcgccctgcg ctgaatgaag gtggtagggc ttcggcagcg
     1921 atatccggtt acacagcctt tgatggagtt cggattgcca tctcagcctg tcctgaatgt
     1981 cacgcccctc cgggtccgac gccatgttac taatgccttg tttgatcacg cattttttta
     2041 cgttcagaag gtttttcagg tatagcgcct tgtagacgtt gccgcgggga tcctgcttga
     2101 gaatgccagt gatcttgaag aggtgaccgg gcggctttgg cggttctgcg atgttcaccc
     2161 ggctgatttc atcaaacggc catgtcgtgc cggcaggaag ccggaaggga atggtttggc
     2221 agtcttcggt caacaatcct gccttatcat aaaaatattt tcgccggatg ccatccggcc
     2281 ccagcttcat aatgggccgg aagcttccgt atctcgtgta cacgataccg cccaataaac
     2341 tgtcagtggg cacggccggc cctttaaagg actttgtgag cgaaaggaga tcttttgcaa
     2401 cctggatagt ttgtaaggcc ggttccggat aaatggttac gatctttccg atttcggtta
     2461 tgccgagcgt cccgttcaga atatcttcgg ctatcgcatg gccaaggggt atcttaaagg
     2521 ccaggtcttt gtcgatcagg taggggatga tcgcttcgat caacggcacg gtttgggaaa
     2581 ggactgcaga caagtgaatg atccacccct gggtggcttg ggtttcgccg acctggaaat
     2641 ggtacccggt cgccttgaaa tttaattcga acggccggag tagcgccgcg tagttgtcgg
     2701 ccggaccgga cggttgcgcg ttttgaggtt ctgggcagtt tattgtggcg gatgacatgg
     2761 ataaggtttg tttgacccga agcaagttac tccggccttg acgaccgatc ggcataaa
//
LOCUS       DATGKE010000066         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1016032, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000066 DATGKE010000000
VERSION     DATGKE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016032"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VKU83_01090"
     CDS             <1..724
                     /locus_tag="VKU83_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92166.1"
                     /translation="NGWQSYGMFNKRPGVGANITWNPTSSFKLLTNDYYGTDAGGLPH
                     RNRFHSDNSMLVRYYNQPEATGISKMALSLTGDIGFEKGDGVNGFSSSSTNGPAQYFL
                     SSMVYNRTWFGRNKFAWTIGGGLMTNPGRYLVLVPTGEASPLPDPNNPTQTEGKFPFD
                     TSPGTEFKAWDCSTNFDWMPNQSVLFRIEFVHRHASVPYFAGSGGVTSPDGYTTTPLP
                     PEWRPDLVQSEDRIILAFLFRL"
     gene            797..925
                     /locus_tag="VKU83_01095"
     CDS             797..925
                     /locus_tag="VKU83_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92167.1"
                     /translation="MDSKRIKGKLAEILVFVIAWLIALSLVYLFIVKLALFKNVFH"
     gene            1123..2841
                     /gene="kdpA"
                     /locus_tag="VKU83_01100"
     CDS             1123..2841
                     /gene="kdpA"
                     /locus_tag="VKU83_01100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HLI92168.1"
                     /translation="MHTEIIGIVASFLITLLLAIPFGRYIAKVFAGERTITDFLNPLE
                     RFIYRIGGVETRKSMNWKDFLKAMLTVNLLWLVYAFVLFLFQGHLPLNPDKNPDMSPD
                     LTFNTAISFLVNCNLQDYSGETGATYLTQLFVITFLQFASAATGIACLVALFNGLKEK
                     TTKNLGNFWVIFIKAITRILLPVALIVAILLVFNGTPASYDGKDTITTLQGDTVQVSR
                     GPAAGMIAIKHLGTNGGGWFGANSAHPLENPNYFTNMLEIITQVLIPIAMIFALGHYI
                     RRKKFAWVVFGIMTLGMLCLLVPTVITEIHGNPEIARMGVVQPTGAMEGKEVRFGPAA
                     SGYWSIVTTIISTGSVNSMHDSSMPLSGLMQMLGMMVNAFYGGCGVGILNYYIYIIIA
                     VFIAGLMIGRTPEFMGHKVEAREVKIAALVTLLSAFIIKGFTALAAYYFVYHANADWA
                     VKPANWLNNPGYHGFSEMLYQFTSTNANNGSGFEGLADNNPFWNITAGLAMIFGRYLP
                     IIGPVAIAGLLAEKKYIPESQGTLKVDSWTFGIMTFATIIIITALSYFPPLALGPIAE
                     QLSMHP"
     gene            2859..4913
                     /gene="kdpB"
                     /locus_tag="VKU83_01105"
     CDS             2859..4913
                     /gene="kdpB"
                     /locus_tag="VKU83_01105"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HLI92169.1"
                     /translation="MPRKNSNKLLNASLVREALKQSVMKLKPAVMMRNPVMFTVYVGT
                     IILLLVCIWIIAGEKSQGSLAYNFTIFVVLLLTVLFANFAEAIAEARGKAQAESLRRT
                     REDTPAKRLNGRQTADGSKWEIIPSLRLKKGDIFLCEAGDIVPMDGEIIEGIATIDES
                     AITGESAPVIRESGGDRSSVTGGTKILSDWIKVRVTISPGESFLDKMIALVEGATRQK
                     TPNEIALTILLASFTIIFLLVCVTLKPFADYANTSITISAFVSLYVCLIPTTIGGLLS
                     AIGIAGMDRALRANVIAKSGKAVETAGDLDTLLLDKTGTITIGNRKATNFYPSGKVDE
                     PVFIEACILASLSDETPEGKSIVELGNSKGIKTPAQADGIRFVPFSAETRFSGVDIGD
                     MSIRKGAFDAILSQVNKAGNAFPEDTDSQVKIISSNGGTPLVVIRNSEVLGVIELQDI
                     IKPGIRERFQRLRKMGVKTVMVTGDNPLTAKFIAAKAGVDDYIAEAKPQDKMDYIRKE
                     QAGGKLVAMMGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDNDPTKLIEVVEI
                     GKQLLMTRGTLTTFSIANDVAKYFAIVPALFITTIPALQALNIMGLRSPQSAILSAVI
                     FNAIIIPLLIPLALRGVAYRPIGAGPLLRRNLLIYGLGGIIVPFLGIKLIDMILALFM
                     QL"
     gene            4928..>5191
                     /locus_tag="VKU83_01110"
     CDS             4928..>5191
                     /locus_tag="VKU83_01110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HLI92170.1"
                     /translation="MKKHILVALRLTAIFIVLCSVIYPLVVAAIARLAPGGGRGETLS
                     LNGKVVGYSNIGQKFDRDIYFWSRPSAVAYNAAGSGGSNKGPTN"
BASE COUNT         1222 a         1313 c         1371 g         1285 t
ORIGIN      
        1 caatggatgg cagagctatg gaatgttcaa caagcggccg ggcgtcggcg ccaacattac
       61 gtggaatcct accagcagtt ttaagttatt gaccaatgac tattacggca ctgatgccgg
      121 tggtctgccg catcggaacc ggttccactc ggacaatagc atgctggtga ggtattacaa
      181 ccaaccggag gccaccggta tctcaaaaat ggcgctttcc cttacgggtg atattggctt
      241 cgagaaaggg gatggggtca atggcttctc cagcagttca accaatggac cggcgcaata
      301 ttttttgagt tcgatggtat acaaccgaac ctggtttggc aggaataagt ttgcatggac
      361 cattggcggc ggactcatga ccaaccccgg ccggtatctg gtgcttgttc ctacggggga
      421 ggcaagtcca ttgcccgatc ccaacaatcc aacgcaaacc gaaggaaagt ttccgtttga
      481 taccagcccc ggaacagaat tcaaagcctg ggactgctcg accaattttg attggatgcc
      541 caatcagagc gttctttttc ggatcgagtt tgttcacagg catgccagcg ttccctattt
      601 cgccgggtcc ggcggagtta cctccccgga tggatacacg acaaccccgc ttccacctga
      661 atggcggcct gacctcgttc agtcggagga cagaattatc cttgccttcc tgttcaggtt
      721 gtaagaagat aaggcgccga tcttttttcg gcagagtcag gcactctttt cgaatatcat
      781 cgcgaaacgt tatctcatgg acagcaaaag gataaaggga aaactggcgg agatcctggt
      841 gttcgtcatc gcctggctga tagcgctgtc gctcgtttat ctgttcattg tgaagctggc
      901 gctgttcaag aacgtgtttc actaaaaata acctttatga ttgcgatcct tttatttctg
      961 ttgtgtcttg tctgcttttg gatcttttac aaatccattg actattttga aaagatataa
     1021 aacgtctgag ccatgatgat atttttgttc attttagcca ttgccgttct cggctatctg
     1081 ctgtacgttt tgcttaagcc cgaaaaattt taatgcccaa acatgcatac cgagatcata
     1141 ggcatcgtcg cctcatttct gataaccctg ctgctggcca ttccgttcgg caggtatatt
     1201 gcaaaagttt ttgcaggtga gaggactatc accgatttct taaatccttt ggagaggttc
     1261 atatacagga ttggtggagt ggagacgagg aaaagcatga attggaagga ctttcttaaa
     1321 gccatgctga cggtcaattt gctgtggctg gtctatgcct ttgtcctttt ccttttccag
     1381 gggcatctgc cgctgaaccc cgacaagaac cccgatatgt cacccgacct gaccttcaat
     1441 acagcgatca gtttcctggt caattgcaat ttgcaggatt attccgggga aacgggcgcg
     1501 acctatctca cgcagctttt tgttattacc tttcttcaat ttgctagcgc ggccacgggg
     1561 atcgcctgcc tggtagccct gttcaatggg ctgaaagaaa aaacgacgaa aaatctggga
     1621 aacttctggg tgatctttat caaggcgata acccgtatcc tgctgccggt cgccctcatt
     1681 gtggcgatac tccttgtctt caacggaacg ccggcaagtt acgacggcaa ggacaccatc
     1741 acgacgctgc agggcgatac cgttcaggtt tcccgggggc cggcggcagg catgatcgct
     1801 attaagcatc ttggcaccaa cggcggcgga tggtttggcg ccaactcggc ccatccgctg
     1861 gagaatccaa attatttcac caacatgctg gagatcatca cccaggtact aattcctatt
     1921 gccatgatat tcgcgcttgg acattacatc cggcggaaaa aatttgcctg ggtcgtgttc
     1981 ggcatcatga cattggggat gttatgtctg ctcgttccca ctgtcatcac ggagatccac
     2041 ggaaaccctg aaattgcccg gatgggcgta gtgcagccca caggcgccat ggaaggtaag
     2101 gaggtgcgct ttggtcccgc cgcttctggc tactggagca tcgtgacgac cattatctcc
     2161 acaggttcgg tgaattcgat gcatgacagc tccatgccgc tttccgggct tatgcagatg
     2221 ctcggaatga tggttaatgc cttttatggc ggatgtgggg tgggcatatt gaactattat
     2281 atctatatca ttatcgccgt gtttatagca ggcctgatga tcggccggac gcccgagttc
     2341 atgggccata aggtggaagc gagagaggtc aaaatagctg ccctggtaac gctgctgtcc
     2401 gctttcatca tcaaagggtt caccgcgctg gcggcttatt attttgtgta ccatgcgaat
     2461 gcggactggg cggtaaagcc ggcgaactgg ctaaacaatc ccgggtatca tggcttctcc
     2521 gagatgttat accagttcac ttcaaccaat gccaataacg gcagcggctt cgaagggctg
     2581 gcggacaaca acccgttctg gaatatcacc gccggtctgg cgatgatctt cggccggtac
     2641 ctgcccatca tcggaccggt agctatcgca gggttgctgg ccgaaaaaaa gtacattccc
     2701 gagtcgcagg gtactttgaa agtggactcg tggacgttcg gcattatgac tttcgccacg
     2761 atcatcatca taacggcgct atcctatttc cctccgcttg cgcttggtcc tattgccgaa
     2821 cagttaagta tgcatccata acccgctaac caacagctat gcccagaaaa aattcaaata
     2881 aactgctcaa tgcttccctt gtaagggaag cgcttaagca atcggtcatg aaactgaaac
     2941 cggcggtcat gatgcggaac cctgtcatgt ttaccgtcta cgtggggaca attattcttt
     3001 tactcgtctg catttggatt atcgccgggg aaaagtcgca gggcagcctg gcatataatt
     3061 ttaccatttt tgttgtcctg ctccttaccg ttctgtttgc caattttgcc gaggccattg
     3121 cggaggcaag aggtaaagcg caggcagaaa gccttcgccg gacgcgcgaa gatactccgg
     3181 cgaaacggct caacggccgc caaacggccg acggctcaaa atgggaaatc atcccgtctt
     3241 tgcggctcaa aaaaggcgat atctttcttt gcgaggcagg cgatatcgtc ccgatggacg
     3301 gagagatcat cgaaggtatt gccactatcg acgagtcggc gattacaggc gaatccgcgc
     3361 ccgtaatccg ggaatccggc ggcgacagaa gttctgttac tggcggcact aagatactga
     3421 gcgattggat caaggtgcgc gttaccatat cacctgggga aagtttcctc gacaaaatga
     3481 ttgccctggt ggaaggtgca acgcggcaaa agacaccgaa cgagatcgcg ctcactattc
     3541 tgctggcttc gtttaccatt atctttctat tggtctgtgt tacacttaag ccttttgccg
     3601 actatgcgaa cacatcgata accatatcgg cgttcgtttc cctatacgta tgcctcatac
     3661 cgactaccat cggcggattg ctgagcgcta tcggcattgc gggcatggac cgcgctctca
     3721 gggctaacgt gattgcgaaa agcggtaaag cggtcgagac ggccggggac ctggatacgc
     3781 tgcttcttga caagacgggt accataacca tcggtaaccg gaaggcaacc aatttctatc
     3841 cttccggaaa ggttgacgag ccggtcttta ttgaagcctg cattctggct tctctttcag
     3901 atgaaacgcc tgaaggaaag tccattgtgg agcttggcaa cagcaaaggg atcaaaacac
     3961 cggcgcaggc tgacggcatc cggttcgttc ccttttccgc ggagacacgg ttcagcggtg
     4021 tggacatcgg cgacatgtct attcggaaag gcgccttcga tgccattctc agccaggtca
     4081 ataaggctgg caacgcgttt ccggaagata cggacagcca ggtaaagatc atatcttcca
     4141 atgggggtac gcctttggta gtaatccgga attctgaagt gctcggtgtc atagaattac
     4201 aggatatcat taaaccgggt atccgcgagc ggtttcagcg gctcaggaaa atgggcgtga
     4261 agaccgtaat ggttaccggt gataatccgc tgaccgcaaa attcattgcc gcgaaagcgg
     4321 gcgtggatga ttacattgcc gaagcgaagc cgcaggacaa aatggactat atccgcaagg
     4381 aacaggccgg cggcaaactg gtagcgatga tgggcgacgg gaccaatgat gccccggcgc
     4441 tggcccaagc cgatgtcgga gtagccatga atagcggtac ccaggccgcc aaagaggcgg
     4501 gcaatatggt ggatcttgac aacgacccta ctaaactgat tgaggtcgta gagatcggca
     4561 agcagttact gatgacgagg ggcacgctta cgacattttc gattgccaac gacgtggcca
     4621 agtacttcgc gatagtgccc gccctgttca tcacgacaat tcccgccctg caggctctga
     4681 acattatggg tcttcgcagt ccccaaagtg cgatcctgtc tgcggtgatc tttaacgcga
     4741 tcattatacc gttgttgatc ccgctggcgc tcaggggcgt ggcgtaccgg ccgattggcg
     4801 cgggaccctt gctaaggagg aacctgctca tttatggact tggaggcatc attgtgccct
     4861 tccttggtat caagctcatc gatatgatcc ttgccctttt tatgcaactc taaaacgaca
     4921 ttcactcatg aaaaagcata tcctggtcgc cttaaggctc accgccatct tcatcgtcct
     4981 ttgttcggta atctatccgc tggtggtagc ggctatagcc aggctggcgc cgggcggagg
     5041 tcgcggtgaa accttgtcat taaatggaaa agtagtagga tacagcaata tcggtcagaa
     5101 gttcgatcgg gatatttatt tctggagccg gccttctgca gtcgcgtata acgccgccgg
     5161 tagcggcggc agcaacaaag ggccgactaa t
//
LOCUS       DATGKE010000067         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_187851, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000067 DATGKE010000000
VERSION     DATGKE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187851"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VKU83_01115"
     CDS             <1..1256
                     /locus_tag="VKU83_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HLI92171.1"
                     /translation="VLQADGQLKTGRDIAIATLLGAEEWGLATTALVVEGCILMRKCH
                     LNTCPVGVATQNPELRKRFTGDADHVVNFFKFITQELREIMAELGFRTIDEMVGQVDS
                     LQVRENVTHWKYGKLDLSPILYKEPAAAGTGLSHVEEQDHGLSAVLDWKLLEAAQPSL
                     DHRQKVFAAFPIRNTDRTVGTILSNEISKRYRSHGLPEDTLHFKFTGTAGQSFGAFNT
                     RGVCLELEGDANDYFGKGLSGARLIVYPSQNAGFVPEENSIIGNVAFYGATSGEAFIR
                     GKAGERFAVRNSGVRAVVEGVGDHGCEYMTGGRIVILGDTGRNFAAGMSGGIAFVYDV
                     RGKFPAQCNKEMVELDPVEAEDIAELKDMIHRHYAYTGSTVAKFVLDDFDNQLQHFVK
                     VFPTDYKKVLRAATNAAAVNRHSNL"
     gene            1271..>2523
                     /locus_tag="VKU83_01120"
     CDS             1271..>2523
                     /locus_tag="VKU83_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HLI92172.1"
                     /translation="MGKPTGFIEYTRHLPSKRPVSERKNDYNEFVHSYSPQELNQQSA
                     RCMNCGIPFCHHGCPLGNVIPEFNDAVYREHWKEAYEILHSTNNFPEFTGRICPAPCE
                     SSCVLGINQPPVAIEEIEKHIIEIAFEQGFVKPNHPPVRTGKKVAIVGSGPAGLAAAD
                     QLNGAGHLVTVFERDDEPGGLLRYGIPDFKLEKWVVARRVKLMQEAGVSFRCNAEVGV
                     NISINDLLREFHAIVLAGGSTIPRDLPVPGRELKGVHFAMDFLKQQNKRVSHLPIDGE
                     HIWATNKNVVVIGGGDTGSDCVGTSNRHKALSVTQFELLPKPPHARTAYMPWPTYPMV
                     LKTSSSHEEGADRHWAIATKEFIGDDEGNLKALRTVRLEWAIGDDGRATRFAEVPGSE
                     RELPCELALLAMGFVHPQHHGLLQEL"
BASE COUNT          575 a          757 c          706 g          485 t
ORIGIN      
        1 tagtcctcca ggccgatgga caactgaaga caggaagaga catcgccatc gccacgttac
       61 tcggtgctga agaatgggga ctggcaacca ccgcccttgt tgtcgaaggc tgtatcctga
      121 tgcgcaaatg ccatctcaat acctgccccg tcggcgtcgc cacccagaac cctgaactcc
      181 gtaagcggtt caccggggac gcggatcacg tggtcaattt cttcaaattc atcacccagg
      241 aacttcgcga gatcatggcg gaactcgggt tcaggaccat tgatgaaatg gtcggccagg
      301 tcgacagcct gcaggtccgc gaaaatgtca cgcactggaa atacggtaag ctggatcttt
      361 cccccattct ctacaaggag cccgcagcgg caggcaccgg cctctcccac gtggaggaac
      421 aggatcacgg actttcggct gtcctggact ggaagctact cgaagcggca cagccgtcgc
      481 tcgaccaccg gcagaaggtt ttcgctgcgt tcccgatccg taacacggac aggaccgtag
      541 ggacgatcct ttctaatgag atctccaaac ggtatcgcag tcacggcttg ccggaagata
      601 ccttgcattt taaattcacc ggcaccgctg gccagagctt tggcgccttc aacacccgtg
      661 gcgtctgcct ggaactggaa ggcgacgcga atgactattt tggcaaaggg ctgtccgggg
      721 caagactgat cgtctatccg tcgcagaatg ccggttttgt tccggaagaa aacagcatca
      781 tcggcaacgt cgctttctat ggcgctactt ccggcgaagc cttcatccgc ggaaaggccg
      841 gcgagcggtt cgcggtccgc aactcgggcg tcagagcggt tgtggaaggc gtgggcgacc
      901 acgggtgtga atacatgacg ggcggtcgca tcgtcatttt gggggacacg ggccgcaatt
      961 tcgcggccgg gatgagcggc ggtatcgcct tcgtctatga cgtccgggga aaattcccgg
     1021 ctcagtgtaa caaggaaatg gtggagctgg acccggtcga ggcggaagac atcgccgagt
     1081 tgaaggacat gatccaccgc cattatgcct ataccggcag caccgtcgca aaatttgtcc
     1141 tggacgattt tgataaccag ctgcagcatt tcgtcaaggt gttcccgacc gattacaaaa
     1201 aagtccttcg cgccgccacc aatgcagcgg ccgtaaaccg tcattctaat ctctaaattg
     1261 acacgtcgaa atgggcaaac caacaggatt tatcgaatat accaggcatc ttccgtcaaa
     1321 gcgtccggta tcagagcgga agaatgacta taatgaattt gtgcactcgt attcgccgca
     1381 ggaactgaac cagcaatcgg cacgctgcat gaactgcggc atcccgttct gtcatcacgg
     1441 ttgtccacta ggaaacgtta tcccggagtt caatgacgcc gtctaccggg agcactggaa
     1501 ggaagcctat gagatccttc actcgaccaa taatttcccc gagttcaccg ggcggatctg
     1561 tccggcgccg tgcgaaagca gctgtgtgct gggcatcaac cagccgccgg tggccatcga
     1621 ggagatcgaa aaacacatca tcgagatcgc ctttgaacaa ggcttcgtca agccgaacca
     1681 tccgcccgtc cgcacgggaa agaaagtagc cattgtaggc tccgggcccg ccggactggc
     1741 cgcggccgac caacttaacg gcgccggcca cctggttacc gtgtttgaac gggatgatga
     1801 acctggtggc ctcctccgct acggaatccc ggatttcaaa ctcgaaaaat gggtagtcgc
     1861 ccgccgggtc aaactaatgc aggaggccgg cgtctcgttc cgctgcaacg ccgaggtagg
     1921 cgttaatatc agcatcaatg acctgctgcg ggaattccac gcaatcgtcc tggccggcgg
     1981 atcaaccatc ccccgcgacc tgccggtacc cggccgggag ctgaaagggg tccattttgc
     2041 catggatttc cttaagcaac aaaacaagcg cgtaagccat ctccctattg acggcgaaca
     2101 tatctgggct acgaacaaga atgtcgtagt catcggcggc ggcgataccg gcagcgactg
     2161 cgtcggaacc tccaaccgcc acaaggcact atccgtgacc caattcgagc ttctgccaaa
     2221 gccaccgcac gcccgcacag cctatatgcc ctggccgacc tacccgatgg tcctcaaaac
     2281 ctcttcctcg catgaggagg gcgccgaccg gcattgggcc atcgccacga aggaatttat
     2341 cggcgacgac gagggaaacc tcaaggcctt gcgcacggtc cggctcgaat gggccattgg
     2401 cgacgacggc cgggcgaccc gctttgccga agtgccgggg agcgagcggg aacttccctg
     2461 tgagctcgcc ctccttgcaa tgggcttcgt ccacccccaa catcatggcc tcctgcagga
     2521 act
//
LOCUS       DATGKE010000068         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1432871, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000068 DATGKE010000000
VERSION     DATGKE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432871"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VKU83_01125"
     CDS             <1..712
                     /locus_tag="VKU83_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HLI92173.1"
                     /translation="LLHDGEIVFDLDKSTHFLALGHNRYPVVVAENGRYHRKYFEWGV
                     IAEFMDTPEKIKERRKSMVNARSEKILVDRRSFWNRIRQKRCLIPVTGIYEHREISGW
                     KKKVPYHIHLRDRPMFCIPGLYHYNTKMPSDPETGEIRGTFTLITRAANSVMRQIHNS
                     GENAYRMPLFLPRDLELQWLQPDLTDEQLAELLAYEMPSEQLVYEPVFSIRGRTDRPD
                     GKPKNAIFEYANLPPILC"
     gene            746..1147
                     /locus_tag="VKU83_01130"
     CDS             746..1147
                     /locus_tag="VKU83_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92174.1"
                     /translation="MKKTSLALALLTIAVLLTAGANAQTDASPVKNTIWKAWLDQLND
                     SIALHITDDSSFVTTSNGDTVVRSVCKMSADTLLLSDYDGQYACTGITGKYKVSFDND
                     VMTTALIEDACEGRAGSINNLKWRKSQPATK"
     gene            complement(1223..1675)
                     /locus_tag="VKU83_01135"
     CDS             complement(1223..1675)
                     /locus_tag="VKU83_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92175.1"
                     /translation="MRKMFMTMSIIAVSVFMGIGAFAQGKPYDKDAIEKQARIGEDSV
                     KLARLKALLPQYEKDKQEAAGQAQQSADANKEAADKLSNDPQDKKLAKKAARAASLAR
                     SDAKKARQASDKLDNLNKDIKKLAKELEKEQAKLQKYEAGLKADSVGN"
     gene            1817..>2633
                     /locus_tag="VKU83_01140"
     CDS             1817..>2633
                     /locus_tag="VKU83_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI92176.1"
                     /translation="MTTSRRDFVRNLSLASASLLSSPAEALAKGGHAASSASLTQPAG
                     RIRTAGAAKDRIRLAVIGTGFRGESHIALALRRTDTDIVAVCDVDAEMLRRTSEIFRK
                     AGKPMPRVFTGSPYAYRDLLATKDIDAVLIATPWEWHAPMVLDSIAAGIKYVATEVIL
                     GITLQDHWDVVNAAEKNNAHVMMLENVCYRRDVMAALNMVRQGIFGELLHLQGGYQHD
                     LRTVLFNDGKPPYDKGVEFGPKAFSEARWRTEHSVHRNGDLYPTHGVGPLAMMI"
BASE COUNT          548 a          812 c          683 g          590 t
ORIGIN      
        1 cctgttacac gatggcgaga tcgtgttcga cctcgataaa agcactcatt ttctggcgct
       61 cggccataac cggtacccgg tcgtcgtcgc cgaaaacggc cgttaccacc ggaaatattt
      121 cgaatggggg gtcatcgccg aattcatgga tacgccggaa aagatcaagg agaggcgaaa
      181 atcgatggtc aacgcgcgca gcgagaagat cctcgtggac cgccgctcgt tttggaaccg
      241 catccgccaa aagcgctgcc tcatccctgt taccgggatc tatgagcacc gggagatcag
      301 cggctggaag aaaaaggtcc cctatcatat tcacctccgg gacagaccga tgttctgcat
      361 cccgggcctc tatcattata acacgaaaat gccctctgac ccggagaccg gggagattcg
      421 cggcacgttt acgctcatca cccgcgctgc caattccgtc atgcggcaga tccataacag
      481 cggcgaaaat gcttaccgga tgccgctctt tcttccccgc gacctggaac tgcagtggct
      541 gcagcccgac ctgaccgatg agcagctcgc ggagcttctt gcctatgaaa tgccttcgga
      601 gcaactggtt tacgaaccgg tgttttcgat ccgtggccgg acagaccggc ctgatggcaa
      661 gccaaagaat gccatttttg aatacgctaa cctgccacct atactttgct aacaaaccgt
      721 tttactcacc accaaaataa cagttatgaa aaaaacctcc ctggcccttg ccctcctgac
      781 cattgccgtc ctgctcaccg ccggagccaa tgcccagaca gacgcttcac ccgtcaagaa
      841 tacgatctgg aaggcatggc tcgatcagtt gaacgacagc atcgcgctcc atatcaccga
      901 tgactcttcg tttgtgacga ccagcaacgg cgacaccgtc gtccgttcgg tctgcaaaat
      961 gtcggcggat accctactgt tatccgatta cgacggccaa tacgcctgca cgggaataac
     1021 aggaaaatac aaggtctcgt tcgacaacga tgtcatgacg acggccctca ttgaagacgc
     1081 ctgtgaagga cgggcagggt cgataaataa tttaaaatgg agaaagtcgc aacctgcgac
     1141 caaatgatga agtccggacc gtgcaggcgc tccagcgcct gcacttattc cctgcggata
     1201 gttggcgcag ggtcccgtgc tgctagttcc cgacgctgtc ggccttaagg ccggcctcgt
     1261 atttctgtag tttggcttgc tccttttcga gctcctttgc cagctttttt atgtccttgt
     1321 tcaggttgtc cagcttgtcc gacgcctgcc tggccttttt ggcatcgctt cttgccagcg
     1381 aagccgccct ggctgctttt ttcgccagct ttttgtcctg cggatcgttg cttagcttgt
     1441 cggccgcctc cttattcgca tcggcggatt gctgcgcctg gccggccgcc tcctgtttgt
     1501 ccttctcgta ttgcggcagc agcgctttca gcctggcaag tttgacactg tcctcgccga
     1561 tccgggcttg cttttcgatc gcatccttgt cgtaaggctt gccctgggcg aacgcgccga
     1621 tgcccatgaa gaccgacacg gcaatgatcg acattgtcat gaacatcttt ctcatatcgc
     1681 agtgattggt tatctctaaa tgtaccacaa tttttccgtt ctgccgcgcc tcgggcatcc
     1741 cgacaacggc cgggcgatga tccgcggcca ttatttgcca ttaagttccg atatttagct
     1801 cataaaacaa ttcatcatga ccacttcccg tcgcgacttc gtccgcaacc tttccctcgc
     1861 ttccgccagc ctcctgagtt ctcccgccga agctttagcg aaggggggcc acgccgcatc
     1921 gtccgcttcc cttacgcagc cggcaggccg gataagaacc gccggagccg ccaaagatcg
     1981 cattcgcctc gccgttattg gcaccggttt ccgcggcgaa agccatatcg cgctggcgct
     2041 ccgccgaacc gatacggata tcgttgccgt ctgcgacgtc gacgcggaaa tgctgcggcg
     2101 caccagcgag atcttcagga aggccggcaa gccgatgccc agggtattta ccggaagtcc
     2161 gtatgcctac cgggacctcc tcgcgacaaa agatatcgac gccgttctta tcgctacacc
     2221 ctgggagtgg catgccccca tggtcctcga ctcgattgcc gccggcatca agtatgtcgc
     2281 tacggaagtc atcctgggaa ttaccctgca ggaccactgg gacgtggtca acgccgcgga
     2341 aaaaaataac gcccatgtca tgatgctgga aaatgtctgt taccgccgcg acgtgatggc
     2401 ggccctgaac atggtccggc agggaatctt cggcgagctg ctccatctgc agggcggtta
     2461 ccagcacgat ctccggaccg tcctcttcaa tgacgggaag ccgccatacg ataaaggtgt
     2521 cgaattcggt ccgaaggcgt tttccgaggc ccggtggcgc accgaacatt cggtccatcg
     2581 caatggcgac ctgtacccga cccatggcgt cgggccgctt gccatgatga tcg
//
LOCUS       DATGKE010000069         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_291270, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000069 DATGKE010000000
VERSION     DATGKE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291270"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VKU83_01145"
     CDS             complement(<1..293)
                     /locus_tag="VKU83_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92177.1"
                     /translation="MTSQQIAKLISEGRFPGQMGRPELVETHISWVLLCERYAYKIKK
                     PIRYSFLDFSTLEKRRYYCEKEIELNRRLTHDLYLDVQAIVEKSGQLSVGDREG"
     gene            complement(326..973)
                     /locus_tag="VKU83_01150"
     CDS             complement(326..973)
                     /locus_tag="VKU83_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92178.1"
                     /translation="MKNGHSLSFTVLSLVLTFVLPLLISCGPSVQLTGSWSDRSIQPV
                     RFTKVMVMAIGNDLEKRRLGENGIKAELQKRGFTAVTSLDEFGPDFAKMKDSVQMRRI
                     LRDKQFDGALTIRVLNVNERDRWVPGGYYYGPVGFYHGFYGYYYRVWGYYDEPGYVIR
                     DVEVLLESNLYRVDSGELLWSGQSKAFSRDPTPQMATRYAKNIVNDMIARGVIMR"
     gene            complement(992..1753)
                     /locus_tag="VKU83_01155"
     CDS             complement(992..1753)
                     /locus_tag="VKU83_01155"
                     /inference="COORDINATES: protein motif:HMM:NF004818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI92179.1"
                     /translation="MERIFENKVAIVTGGSFGIGQATALAFAKRGAKVVVADWIEDKE
                     QATLKQIEAAGGDALIVKCDVSKAADITAMIDKAFSAYGRLDFAFNNAGIEGVSAVTQ
                     ECTQDNWDRTIAINLTGIWLCMKHELPHMLREGKGAIVNCASVAGLIGFAGLPAYVAT
                     KHAVVGLTKTAALENAKLGVRVNAVCPGVIRTAMIDRLTGKDKVAEQQFVNMEPVGRF
                     GEPAEVAEAVVWLCSDAASFVTGHAMPVDGGWIAS"
     gene            complement(1763..>2307)
                     /locus_tag="VKU83_01160"
     CDS             complement(1763..>2307)
                     /locus_tag="VKU83_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLI92180.1"
                     /translation="LVDISLVEQVLDAGRMAPSAMNKQPWQFYVLANKETIQSFSREI
                     AGVMMKGAFKSGVAGIAQLMKAASGLLHLSHGIDLHAIKDPVFYGAPIVIFLTAPRDN
                     EWASLDVGMCAQNIMLAAKSLGLDTCPVGFGKYVEKTKIFSRLHVPASEEVKLAIILG
                     YGDETPEVHERNKNNLLFID"
BASE COUNT          538 a          679 c          497 g          593 t
ORIGIN      
        1 ccttcccggt cgccaacaga taattgaccc gatttttcca caatcgcctg cacatcgagg
       61 tataagtcat gggtaagcct tctgttcagc tcaatctctt tttcgcagta gtatcttcgt
      121 ttttccaggg ttgagaagtc aagaaaagaa tagcggatcg gtttcttaat tttataggcg
      181 taccgttcgc ataacaatac ccaggaaata tgcgtctcaa caagctccgg ccggcccatc
      241 tgacccggaa acctcccttc agaaatcaac ttagcaatct gctggctggt cattttcgat
      301 gctatcggta aacatacccc cttttttaac gcatgattac ccctctggca atcatgtcat
      361 tcacgatatt ctttgcatac cgggttgcca tttgaggcgt aggatcccga ctgaatgctt
      421 ttgactgccc ggaccacaac agttcccccg aatctaccct ataaagattg ctttccaaca
      481 gtacctccac atccctgatc acatagccgg gttcgtcata atagccccat acccggtaat
      541 aataaccgta aaatccgtgg tagaacccaa caggcccata atagtagccg cccggcaccc
      601 agcggtcacg ctcgttcaca ttcagcaccc ggattgtcaa tgctccgtca aactgcttgt
      661 cccgcaatat ccggcgcatt tgaacagaat ccttcatttt ggcaaagtcg ggcccgaatt
      721 catccagact ggtgacagcg gtaaaaccac gcttctgcag ttcggccttt atgccgttct
      781 ctcccagcct cctcttttcc agatcgttgc cgatcgccat gaccatcacc ttggtaaagc
      841 gtacaggctg aatactcctg tcgctccagg agccggtcaa ttgcaccgac gggccgcatg
      901 agatgaggag tggcagcaca aaggttaata ccagcgacag aacagtgaag ctaaggctat
      961 gaccgttttt cataacttct gaatttaggt tttatgaggc aatccatcca ccgtcgaccg
     1021 gcatcgcgtg acccgtcaca aatgaagccg catcggagca tagccaaacc actgcttccg
     1081 ccacctcagc cggttcgcca aatctgccaa ccggctccat attgacgaat tgctgttccg
     1141 ctaccttgtc ttttccggtg agcctgtcga tcatggctgt ccggatgaca cccgggcaca
     1201 ctgcattcac ccgtacgccc agctttgcat tttccaatgc ggctgtcttg gtaagaccta
     1261 cgaccgcatg tttagtggcc acataggccg ggaggcccgc aaatccgatc agcccggcta
     1321 cagacgcgca gttgacgatc gctcctttcc cttctctaag catgtgcggc aactcgtgtt
     1381 tcatacacag ccagatgcct gtaaggttga tcgcaattgt cctgtcccag ttgtcctgcg
     1441 tgcattcctg tgtaaccgcc gatactcctt caatgccggc attattgaaa gcaaaatcca
     1501 accgtccgta ggctgaaaag gctttgtcga tcattgccgt aatgtcagcg gcctttgaaa
     1561 catcgcattt cacaataagc gcgtctcctc ctgccgcttc gatctgcttt agcgtcgcct
     1621 gctctttatc ttctatccaa tctgcgacaa ccacctttgc gcccctcttg gcgaaggcca
     1681 gggctgtcgc ctggccgata ccaaaactgc cgccggttac aatggctact ttgttctcaa
     1741 agatcctttc catagcgatt tttcaatcga tgaataataa gttattcttg ttcctttcat
     1801 gcacttccgg tgtctcatcg ccatacccaa gaatgatcgc cagctttact tcctcggacg
     1861 ctgggacatg aagcctggaa aaaatcttgg ttttttccac gtattttcca aaacccacag
     1921 gacaagtatc caatcccagg gactttgccg ccagcatgat attctgtgca cacatcccga
     1981 catccaggga agcccattca ttgtcccgcg gggcggtaag aaatatgact atcggcgccc
     2041 cataaaaaac ggggtctttt atcgcgtgca gatcgatccc atgcgaaaga tgcaggagcc
     2101 cggatgctgc cttcattagc tgggcaatac cggctacgcc ggatttgaac gctcctttca
     2161 tcatgactcc cgcaatctcc cgggaaaagg attggatggt ttctttgttg gcgagaacat
     2221 aaaactgcca tggttgcttg ttcatggccg aaggcgccat tcgccctgca tccaatacct
     2281 gttcgaccag gctgatgtcc actaact
//
LOCUS       DATGKE010000070         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1122128, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000070 DATGKE010000000
VERSION     DATGKE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122128"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..504
                     /locus_tag="VKU83_01165"
     CDS             166..504
                     /locus_tag="VKU83_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92181.1"
                     /translation="MTDDKQADAQESGATDETTGVEGQIKRIQDKLQQLLRQRDLLLK
                     ENGKLKAELQRARESQTEGDSRLGQLQQQIEALRVTRTDMSEGEKRELEKRLGQYIRE
                     IDRCIALLGE"
     gene            complement(510..764)
                     /locus_tag="VKU83_01170"
     CDS             complement(510..764)
                     /locus_tag="VKU83_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92182.1"
                     /translation="MFLKVKSPVFIDHLTLQVGEPQKCMLRAVLRDDTGIVCSALETE
                     VEKDQQELDWNGLNDLPYGVYTLEIFRGQDEMKLRLVKRV"
     gene            907..1188
                     /locus_tag="VKU83_01175"
     CDS             907..1188
                     /locus_tag="VKU83_01175"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HLI92183.1"
                     /translation="MQDLMTINVVIGDRTYRIKIEVADEEVVRRTLKTINDKIIEYKT
                     QFAGRDMQDYIAMVLLWYATQAVRQGDAGAGRPLLEALSRIEQQLDRAL"
     gene            1235..2788
                     /gene="rny"
                     /locus_tag="VKU83_01180"
     CDS             1235..2788
                     /gene="rny"
                     /locus_tag="VKU83_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HLI92184.1"
                     /translation="MESIIAGVIALVIGIIAGKLLFAKNTTKKLEDAELQAKKMISDA
                     QTTAENIKKEKQLEAKEKFVQLKAEHDKEVLEKNRRINDAEARIKQKEQSINQKLENL
                     DKQAKDNDTIKENLTRQIEVVNLKRTELEKHQEEHIRRLEKIAALTAEEARSQLIESL
                     RQETQTRALAMQQEIIDDARQKANKEARKIIIQSIQRTAAEQAIENSITVFNLESDEI
                     KGQIIGREGRNIRAIEAATGVDLIVDDTPEAIVLSSFDPLRREIARLSLQRLVTDGRI
                     HPARIEEIVEKTRKQIEEQVMEIGERTVIELGIHGLHKELVRIVGKMRFRSSYGQNLL
                     MHSREVANLCGIMASELGMNPKLAKRAGLLHDIGKVPDEETELSHALLGMKLAEKYGE
                     NPAVVNAIGAHHDEVEMQYVIAPIIQACDAISGARPGARREIMQQYLQRIKDLENLAL
                     GYPGVEKAYAIQAGRELRVIVEADKVTDVDSDKLSFEIAQKIQTEMTYPGQIKVTVIR
                     EKRAVNVAR"
     gene            3023..3388
                     /gene="rplS"
                     /locus_tag="VKU83_01185"
     CDS             3023..3388
                     /gene="rplS"
                     /locus_tag="VKU83_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLI92185.1"
                     /translation="MNPAIAFIHEQMTGKREIPNFKAGDNITVNYKIIEGNKERIQGF
                     KGEVIKRQGEGHTATFTVRKISDGVGVERTFPLFSPNIESVELNKVGRVRRAKLYFQR
                     ERSGKSARIKEKRMAVTGK"
     gene            3460..3729
                     /locus_tag="VKU83_01190"
     CDS             3460..3729
                     /locus_tag="VKU83_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLI92186.1"
                     /translation="MGDLFSGPHIILIALVVLLLFGGKKIPELMRGLGRGIREFKDAK
                     DNVQREIEEHVNEDRKTTAATNQPTTVQTTTAATKQVSSDSSIHN"
BASE COUNT         1039 a          954 c         1087 g          694 t
ORIGIN      
        1 agctgggcgc cggccaacgg tcccttgctc tcagcctgac ctttctcgat gaagagaaga
       61 ccctgacgga taaagaaatt gacgggatgg tggccaggat catgacggca ctggaacgtg
      121 aagtaggcgc cgagatccgg aaataagcag gtatcaaacg ttcatatgac cgacgataag
      181 caggcggacg cgcaggagtc cggcgcgacc gacgaaacca ccggagtgga aggacagatc
      241 aaaaggatcc aggataagct gcagcaactg ctgcggcaac gcgacctcct gctgaaggag
      301 aacggaaaat tgaaagccga gctccaacgg gcccgggaaa gccagacgga aggcgacagc
      361 aggctgggtc agctgcaaca gcagatcgag gcgcttaggg taaccagaac ggatatgagt
      421 gaaggggaaa aaagggagtt ggagaaaagg ctggggcaat acatccggga aatcgaccgg
      481 tgcatcgcgt tgttagggga atgatccggt cagactcttt tcaccaaccg tagtttcatt
      541 tcatcctggc cgcggaagat ctccaacgta tacacgccat acggcagatc gttaagaccg
      601 ttccagtcca gctcttgctg gtctttttca acctctgtct cgagcgcgct gcagacgatt
      661 ccggtgtcat cccggagtac ggcgcgtaac atgcattttt ggggttcgcc cacctgcaac
      721 gtgaggtggt cgatgaagac gggggacttg acttttagga acatggctaa gtttttagat
      781 agggtattgg tgagcaatct tagttttaaa tcaggttatg aattaggtca aaaaaacaat
      841 caacaacctc tgatcaggat ccccttatac acaggtgtgg ataaatatat aacaactatt
      901 tgatgtatgc aagacctaat gacaattaat gttgtgatag gtgatcgcac ttaccggatc
      961 aagatcgagg tcgcggacga ggaggtggtc cggcgcaccc tgaagacgat caatgataaa
     1021 attattgaat ataagaccca gtttgccggc cgggacatgc aggactatat cgccatggtc
     1081 ttgttatggt acgccaccca ggcggtccgg cagggggatg ccggggcagg aaggccgctt
     1141 ctggaggccc tgagccggat cgaacagcag ttggacaggg cgttataagt atttttccta
     1201 tcttcgccaa ctaacataaa acagacacta cctaatggag tcaataatag cgggagtcat
     1261 agccctggtg ataggaatta tcgccgggaa gctccttttt gcgaagaaca cgaccaaaaa
     1321 gctcgaggat gccgagttgc aggcaaaaaa gatgatttcg gacgcacaaa cgacggccga
     1381 gaacataaag aaggagaaac agctggaagc caaggagaaa ttcgtccagc ttaaggcgga
     1441 acacgataag gaagtcctgg aaaagaaccg ccggatcaac gacgcggagg cccggatcaa
     1501 gcagaaagaa cagagtatca atcaaaaact cgaaaacctc gacaagcagg ccaaagacaa
     1561 cgatacgatc aaggagaacc tcacccggca gatcgaggtg gtgaacctca agcgcacgga
     1621 gctggaaaag caccaggagg agcatatcag gcggctggaa aagatcgccg ccctgacggc
     1681 ggaagaagcc aggtcccagc tgatcgagag cctgcggcag gagacccaga cccgggcgct
     1741 ggccatgcag caggagatca tcgacgacgc ccggcagaag gccaataaag aggcccggaa
     1801 gatcattatc caaagcatcc agcggaccgc tgcggagcag gcgatagaaa actccatcac
     1861 ggtattcaac ctcgaaagcg atgagatcaa gggccagatc atcggccgcg agggccggaa
     1921 tatccgggcc atcgaagccg cgaccggcgt agaccttatc gttgatgaca cgccggaagc
     1981 catcgttctg agctcgttcg acccccttcg ccgggagatt gcccgcctgt cgctccaacg
     2041 gctggtgacc gacggccgga tccaccccgc ccgcatcgag gagatcgtcg aaaagacgcg
     2101 gaaacagatt gaggaacagg tcatggagat cggggaacgg acggttatcg aattggggat
     2161 ccacggtctc cacaaagagc tcgtccggat cgtcggaaag atgcgattcc ggtcttccta
     2221 tggccagaac ttattgatgc acagccgcga agtagccaac ctctgcggca ttatggcttc
     2281 ggagctgggc atgaacccca agcttgccaa gagggcgggg ctccttcatg acatcggcaa
     2341 ggtacccgac gaggagactg agctcagcca tgccctgttg gggatgaagc tggcggaaaa
     2401 atacggggag aatccggcag ttgtcaatgc cataggcgct caccacgacg aggtggaaat
     2461 gcagtacgtg atcgcgccga tcatccaggc ctgcgacgcg atcagcggcg ccagaccggg
     2521 cgcgcgccgc gagatcatgc agcaatacct tcagcggatc aaggacctgg aaaacctggc
     2581 cctgggctac ccgggcgtcg aaaaagccta tgccattcag gccggccgtg agcttcgggt
     2641 tatcgtcgaa gccgataagg tgaccgatgt ggacagcgat aagctcagct ttgagatcgc
     2701 ccagaagatc cagacagaga tgacctaccc cgggcagata aaagttaccg tgatccgcga
     2761 gaagcgcgcg gtgaatgttg caagatagcc gggcagcccg cggcaacgcc ctgcatcaac
     2821 ggcctacatc aacgccctgc atcgacgccc cgcggaaacg ctccccggaa gagctccgca
     2881 gcattgcccc gcggcgataa gcgggcgggg cctgaaaatt tttggacttt ccgctcaatc
     2941 ttgctacctt tgccatcctt ttgaaagccg ccggaagcgg ctcgtcctaa gcaccattat
     3001 tagcgattta aaagattatg ttatgaaccc tgctatagct tttatccacg agcaaatgac
     3061 cggaaaaagg gagatcccca acttcaaagc cggtgataac atcaccgtga actataagat
     3121 cattgaaggc aacaaggagc ggatccaggg attcaagggc gaggtgatca aacgccaggg
     3181 cgaggggcat accgccacct ttaccgtccg aaagatctcc gacggggtag gcgtcgaacg
     3241 gaccttccct ttattttccc cgaatatcga gtccgtcgaa ctgaacaaag taggccgcgt
     3301 ccgcagggcc aagctgtact ttcagcgtga gcgaagcggc aagagcgcca ggatcaaaga
     3361 gaagagaatg gcggttacgg gcaagtaagg gccgaaggtt aaatatcttt ttgttatcaa
     3421 tttatctgtg tacctttagg agtactttaa aatgtagtta tgggagattt attcagtggg
     3481 ccgcatatta ttctgattgc actggtagtg ctgctattgt ttggcgggaa aaaaatacct
     3541 gaactgatgc gcggactcgg cagaggtatc cgcgagttca aggacgcgaa ggacaatgtg
     3601 cagcgcgaaa ttgaagagca tgtgaacgag gacaggaaga cgactgccgc aacgaatcag
     3661 ccgacaacgg ttcaaacaac cacagccgct accaaacagg tttcctcgga ttcctcgatc
     3721 cataattaaa cgatttcgct cggtatcgag tgtacgaacc tcgcaatttt tgcg
//
LOCUS       DATGKE010000071         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_811258, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000071 DATGKE010000000
VERSION     DATGKE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811258"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VKU83_01195"
     CDS             complement(<1..528)
                     /locus_tag="VKU83_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HLI92187.1"
                     /translation="MKRHLRTAFIVWIALLPALIASAQVKFTTVVSSQDLGQRDYLQV
                     QFIVENAQQIEQFDPPTDFPGFTILEGPIQNSGMSVINGNMSQYKGLSFVLQPTHTGR
                     FTVAGATAVVNGQKMRSNNVTVTVHPGGGSASGPPANSPFGGFPGNPLAQPFGPDPFA
                     PSEPGMADRDDVLRPG"
     gene            complement(583..1284)
                     /locus_tag="VKU83_01200"
     CDS             complement(583..1284)
                     /locus_tag="VKU83_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI92188.1"
                     /translation="MNCVITGASRGLGKAIAGAFAAQGYDLVLSSRDPTALSAASREL
                     AGRYPEISVRSRPADLGDREQVRALGRWVLEPGLPPDVLVNNAGQFIYGSLSEEEDGV
                     LERLMAVNLYSAYHLTRSLLPSMVARKSGHIFNICSIASLQAYPHGGAYGVSKYALAG
                     FSANLREEMKGFGIKVTAVYPGAAYTDSWAGSGVDPHRIMEAADIAKMVVAAAGLSPQ
                     ATVEEIVLRPQLGDL"
     gene            1305..2000
                     /locus_tag="VKU83_01205"
     CDS             1305..2000
                     /locus_tag="VKU83_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HLI92189.1"
                     /translation="MQYKHLFFDLDHTLWDFEANSRQTLEELYHSLGLYSRGVADFPA
                     FHRRYLEHNDKLWERYRNGFIKVDELRWKRMWLTLLDFKIADEPLARTMDTLFLDALP
                     TRKTLFPYTVEILEYLTGKNYRLHLITNGFEKTQHSKLQGSGISRFFEQVVTSEGSNS
                     LKPHKEIFDYALRVTGAHPAESIMLGDSIEVDIQGAMNAGIDQVYVNHLGTEPAIRPT
                     YMVHTLKQLENIF"
     gene            complement(2001..2660)
                     /locus_tag="VKU83_01210"
     CDS             complement(2001..2660)
                     /locus_tag="VKU83_01210"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HLI92190.1"
                     /translation="MTIHREGYKTIAISALLFALVNLISFYYISAHEFWLSTLIFLVT
                     LATLLFLVSFFRVPVRKWTVGQDLVICPADGKVVVIEEITDLEYFSGKRLQVSIFMSP
                     ANVHQNRNPIGGEVVYSQYHPGRYLVAWHPKSSTENERHSVVIRNSRADILVKQIAGA
                     VARRIVNYLAVGQKVEQSAEMGFIKFGSRVDVLLPPGADVRVKLGEAVQGGVTVLAKL
                     R"
     gene            complement(2699..>3544)
                     /locus_tag="VKU83_01215"
     CDS             complement(2699..>3544)
                     /locus_tag="VKU83_01215"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLI92191.1"
                     /translation="VFVIVMLTGLLWNPWSFFVLFTVIHFGCWIEYRKIMVLIDKEYA
                     AVSLLHRYSVPVAGWCFILYWVPPAFSIGALPVFQIGKWGCLIFLFVMLAGETIQYRT
                     LSIRNILHSFFGLLYISLPVAMLVWLRAVYFGNPIPFLDRMLVLMIIGSIWINDTMAY
                     IVGSLIGRTSLTPVSPKKTWEGTIGGIILAIGAMWLLGWALRDEPAVGLFPPPVWLVV
                     AALASVTGTFGDLLESKLKRMAGVKDSGHILPGHGGFLDRFDSLLIATPFAWAYLVWI
                     VGVGS"
BASE COUNT          836 a         1052 c          971 g          685 t
ORIGIN      
        1 gccgggtctg aggacgtcat cccggtcggc catgccgggt tccgaaggag cgaagggatc
       61 gggaccaaaa ggctgggcca gggggttgcc cgggaaaccc ccgaagggcg aattcgccgg
      121 aggtcctgaa gctgatccgc cgcccggatg tacggtcacg gtgacgttgt tggaccgcat
      181 cttctggccg ttcaccaccg cggtcgcacc ggccaccgta aaccttccgg tgtgtgtcgg
      241 ctgtaaaaca aatgaaaggc ccttgtattg tgacatattc ccgttaatga cgctcatgcc
      301 gctgttctgg atcggacctt cgagaatagt aaaaccgggg aagtcggtgg gaggatcgaa
      361 ctgctcgatc tgttgcgcat tttcgacaat gaactgtacc tgcagataat cgcgctgacc
      421 caaatcctgg ctgctgacca cggtcgtgaa cttcacctgg gcagaagcaa taagggcggg
      481 cagcagggct atccagacaa tgaatgcagt acgtagatgt cgcttcatat caccggaatg
      541 caaatataaa atattcaact atttgccggg atggcatccc agttaaagat ctcccaattg
      601 aggcctgaga acgatttcct cgacggttgc ctgaggggac agtcccgccg cggcaacgac
      661 catcttcgcg atatccgccg cctccataat ccggtgggga tcaactccgc tcccggccca
      721 ggaatcagta taggcggcgc cggggtacac ggcggtcact ttgatcccaa atcccttcat
      781 ttcttcccgc aggttggcgg aaaatcccgc caacgcatat ttgctgacgc cgtaggctcc
      841 gccgtgcggg taggcttgca gggaagcaat ggaacaaatg ttgaaaatat ggcccgactt
      901 cctggccacc atggacggca gcagggaccg ggtaagatga taggcgctgt agaggttcac
      961 cgccatcagc cgctccaata cgccgtcctc ctcttcggac aggctgccat agatgaattg
     1021 gccggcgttg ttcaccagga cgtccggggg caaccccggt tccagcaccc aacggcccag
     1081 tgcgcggacc tgttccctat ctccaaggtc tgcgggccgg gaccgcaccg agatctccgg
     1141 atatcgcccg gcgagctcgc ggctcgccgc cgacaaggct gtgggatcgc gggaggacag
     1201 aacgagatca tagccttgcg ccgcgaacgc acccgcgatg gccttgccaa gtcccctgga
     1261 agctccggtt ataacacaat tcataattaa gtataacttg cggaatgcaa tataaacacc
     1321 ttttcttcga cctggaccat accctatggg atttcgaggc gaatagccgc caaaccctcg
     1381 aagagctgta ccattcgctc gggctttata gccggggggt ggccgatttc cccgcgtttc
     1441 accgccgtta tctcgaacac aacgacaaac tgtgggaacg ataccgcaat ggattcatca
     1501 aggtggacga gctccgctgg aagcggatgt ggctcacgct tctcgacttc aagatcgccg
     1561 atgaaccact tgcccgcacc atggataccc tcttcctgga cgccttgccg acacgcaaaa
     1621 cgctttttcc ctataccgtg gagatcctgg aatacctgac cggcaaaaac tatcgcctgc
     1681 acctcatcac gaatggattc gaaaaaaccc agcatagtaa actgcaaggc tcgggcatca
     1741 gccggttctt tgaacaggta gtcacctccg aggggtccaa cagcctgaag ccgcacaagg
     1801 agatctttga ttacgccttg cgcgtgaccg gcgcccatcc cgctgaaagc atcatgctgg
     1861 gcgacagcat cgaggtagat atccagggcg cgatgaacgc cggcatcgac caggtctatg
     1921 tcaatcacct ggggacggag cccgccatcc ggcctaccta tatggtacat acgctaaagc
     1981 agctggaaaa tattttttga ctatcgcagc ttcgccagca cggtgacgcc gccctgaacc
     2041 gcttcaccca gcttcaccct gacatcggcg ccgggcggca gcaagacgtc cacccttgag
     2101 ccgaatttga tgaagcccat ttccgcgctc tgttcgacct tttgcccaac agccaggtag
     2161 ttcacgatcc gtcgggccac cgcgcccgcg atctgtttga ccaggatgtc agcacggctg
     2221 ttccggatga cgacgctgtg ccgctcgttt tcggtcgaag atttgggatg ccatgcgacg
     2281 agatacctgc cgggatgata ctggctgtaa acgacttcgc cgccgattgg attccggttt
     2341 tggtggacat ttgccggact catgaatatc gatacctgca gccttttgcc cgagaaatac
     2401 tcaaggtccg ttatctcctc gatcaccacc accttcccgt ccgccggaca aatgaccagg
     2461 tcctggccca ctgtccattt gcggaccggt acccgaaaaa aggagacgag gaaaagcagg
     2521 gtcgcaagcg taacaaggaa gatcagcgtg ctcagccaga attcgtgggc gctgatgtaa
     2581 taaaacgaga tcagattgac cagggcgaac agaagggcac tgattgcgat cgttttatag
     2641 ccttcgcgat gaattgtcat tttgcggaat tggacatcaa aggtagctat tttctcactc
     2701 aggacccgac accgacgatc cataccagat aagcccatgc gaaaggcgtc gcgatcagca
     2761 gggagtcgaa cctgtccagg aacccgccat gccccggcag gatatggccg ctgtccttta
     2821 ctcccgccat gcgcttgagc ttgctttcca ggagatcgcc gaaggtaccg gtgacggatg
     2881 ccagcgctgc gaccaccaac cagaccggcg gcgggaacag gccgaccgcg ggttcatcgc
     2941 gtaaagccca gcccaggagc cacattgcgc cgatggccag gatgatgccg ccgatcgtcc
     3001 cctcccacgt ctttttcggc gacacggggg tgagcgaagt ccttccgatc agggagccga
     3061 caatataggc catcgtgtcg ttgatccaga tcgacccgat gatcatcagt accagcatcc
     3121 ggtcaaggaa cggaatcggg ttcccaaaat agaccgcccg taaccaaacc agcatcgcga
     3181 cgggcaggga aatatataac aggccaaaga aggagtgcag gatattccgg atgctcagcg
     3241 tccggtactg gatcgtttcc ccggcaagca tcacgaacag gaagatcagg caaccccatt
     3301 tcccgatctg gaagaccggg agcgcgccga tgctgaacgc cggcggaacc caatacagga
     3361 tgaagcacca gccggcgacc ggaacgctgt accggtgcag gagcgaaacc gccgcatatt
     3421 ccttgtcgat gagcaccatg atcttccggt actcgatcca gcatccgaaa tggatgaccg
     3481 tgaacaaaac aaaaaagctc cacggattcc acaacaagcc ggtcagcatg acgatcacaa
     3541 agac
//
LOCUS       DATGKE010000072         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_811158, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000072 DATGKE010000000
VERSION     DATGKE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811158"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VKU83_01220"
     CDS             <1..889
                     /locus_tag="VKU83_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HLI92192.1"
                     /translation="PGLLGGAADLAPSTETHLNGLDDFDAEHPGGRNFHFGVREHAMG
                     SILNGMAVTKGLMPFGATFLIFSDYMRPPIRLAAIMRIKPILIYTHDSIGLGEDGTTH
                     QPIEQLANLRAIPHVTVIRPADANETVQAIKLALGHRGGPVILVLTRQNIPIIEANAY
                     RTTGDLSKGAYILSEPERAPELILIASGSEVDLILKAQRKLNENGIAARVVSMPSWEL
                     FEKQDDSYKQRVFPKGLRKRLAVEAASPMGWHKYTTDEGDMLGMTRFGESAPAADLFK
                     EFGFTVENVVNRAKSLLGK"
     gene            complement(904..1317)
                     /locus_tag="VKU83_01225"
     CDS             complement(904..1317)
                     /locus_tag="VKU83_01225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HLI92193.1"
                     /translation="MPLLEKQLVVKRSTLKGAGRGLFTTKAIPKGKKIVEYKGKVTKW
                     KEVNDRHGLNGYIYYVNRNHVIDAWGSKTALGRYANDAKGLFRSEGLSNNCTYRIEGL
                     RVFIYSMRSIPAGSELFVGYGKEYWDIIKENKGIR"
     gene            1392..>2061
                     /locus_tag="VKU83_01230"
     CDS             1392..>2061
                     /locus_tag="VKU83_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLI92194.1"
                     /translation="MQDPTIPQEVGTPDLENKQAMLAAIIDSSEDAIISKDLDGYITS
                     WNPAAERMFGYTGKEAIGRHISMLIPADKLGEEDMIISRIRSGLRVEPFQTVRVTKSG
                     SLIPISLTISPIKACDGRIIGASKIARDITGQVRAQQELEAHARIQELLLSAGSILAA
                     NLDLQSILQKVTDITTQLSGAEFGAFFYNMVNDGGDSFWLYTLSGQGISRDTFKDFPV
                     PRKTP"
BASE COUNT          482 a          608 c          537 g          434 t
ORIGIN      
        1 cccgggtctg ctgggcggcg ccgctgacct ggcccccagc accgagaccc acctgaacgg
       61 actggacgat ttcgacgcgg aacaccccgg tggacgcaat ttccattttg gcgtccggga
      121 acatgccatg ggctcgatcc tcaatggcat ggcggttacc aaagggctca tgcctttcgg
      181 cgccacattc ctcatcttct cggattatat gcggccgcct atccggctgg cagctatcat
      241 gcggatcaag cccatcctca tctataccca cgacagtatc ggactgggag aggatggcac
      301 gacccaccag cctatcgaac aactcgccaa cctgcgcgct atcccccatg tcacggttat
      361 ccggcctgcg gacgccaacg aaaccgtaca agcgatcaaa ctcgccctcg gacatcgcgg
      421 cggtccggtc atccttgtcc tcaccagaca gaatatccct atcatagagg cgaatgccta
      481 ccggactacc ggcgatctca gcaaaggtgc ctatatcctg tccgaacccg aacgtgcacc
      541 cgagctgatc ctgatcgcga gcggctccga agtggacctc atcctcaagg cgcagcgcaa
      601 gcttaacgag aatggcatcg ccgcgagagt ggtcagcatg ccgtcctggg aactcttcga
      661 gaaacaggac gacagttaca aacagcgggt ctttccgaaa gggcttagaa aacgcctggc
      721 cgtggaagca gcttcgccca tgggctggca taagtatact accgacgaag gcgatatgct
      781 gggaatgacg cggttcggcg aatcggcccc ggcagcagac ctctttaaag aattcggctt
      841 taccgtggag aatgtggtga acagggccaa gtccctgttg ggtaaataag gtgggaatgc
      901 accctaccgg atccccttgt tctctttgat gatatcccaa tattccttcc catagcctac
      961 aaaaagctcg ctgccggccg gaatagaacg catcgaatag ataaaaacac gcaggccttc
     1021 gatgcggtaa gtgcaattat tgctaagccc ttccgaccgg aacaacccct tcgcatcatt
     1081 cgcataacga ccaagggcgg tcttgctgcc ccaggcatcg atcacgtgat ttcggttcac
     1141 gtaataaatg tacccattca gaccgtggcg gtcattgacc tccttccatt tggtgacctt
     1201 tcctttgtat tctacgatct ttttgccctt agggatcgct ttggtggtga acagccccct
     1261 tccggcgcct ttgagagtgg atcttttgac gaccagctgt ttttcgagta acggcatgaa
     1321 taatttgaag attgagcttg atccgccgta agcggattgt aaaaataggg gattcccgta
     1381 aattcgcaat catgcaagac ccgactatcc cgcaagaagt tggaacccct gatctggaga
     1441 acaaacaggc gatgctggct gccattatcg attcctcgga agatgccatt atcagcaagg
     1501 atctggatgg ttatattacg agctggaacc ccgcggcgga aagaatgttc ggctacaccg
     1561 ggaaagaagc gatcggcagg catatctcga tgttgatacc cgccgataag ctcggtgaag
     1621 aggatatgat catttcccgg atcaggagcg gtctgcgggt cgagcctttt caaacggtcc
     1681 gcgttaccaa aagcggaagc ctgatcccca tctccctcac catttcgccg atcaaggcgt
     1741 gcgacggccg gatcatcgga gcctccaaga tagcccggga tatcaccggt caggtacggg
     1801 cgcagcagga actcgaggcc cacgcccgta tccaggagct gctgctttcc gcaggaagca
     1861 tcctggccgc caacctggac ctgcaatcga tcctgcaaaa agtgaccgat atcaccaccc
     1921 agctctccgg cgcggagttc ggcgctttct tctacaacat ggtcaatgac ggcggcgatt
     1981 ccttctggct gtatacctta tcggggcagg ggatatcgag agataccttc aaagacttcc
     2041 ccgtgccgcg caagacccca c
//
LOCUS       DATGKE010000073         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1531302, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000073 DATGKE010000000
VERSION     DATGKE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531302"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VKU83_01235"
     CDS             complement(<1..344)
                     /locus_tag="VKU83_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92195.1"
                     /translation="MLLEVRQQYSFYGVLLYVASTIFVLYLAMGQPEAMVWKGLFWMI
                     QLFVCVNAVAKSFLQESRGRLLYFYSITGAANFILSKLLFSALLMLLMSLLSLGLFVF
                     MLGNPMPEPLLFL"
     gene            complement(529..1101)
                     /locus_tag="VKU83_01240"
     CDS             complement(529..1101)
                     /locus_tag="VKU83_01240"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HLI92196.1"
                     /translation="MFIEPHLRAEKRGWIEVICGSMFSGKTEELIRRLKRAKIANLRV
                     EIFKPAVDTRYHEENVVSHDENAISSTPISTSTSILLMAADVEVIGIDEAQFFDDQLP
                     EVCDQLAIRGVRVIVAGLDMDYRAKPFGQMPNLLAKAEYITKLHAICAKCGNIANYSF
                     RKTRVSPQLLLGEKDVYEPRCRKCFYGLPE"
     gene            1289..2128
                     /locus_tag="VKU83_01245"
     CDS             1289..2128
                     /locus_tag="VKU83_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92197.1"
                     /translation="MERLKELISQLTEQVDHQAEPAEMLQTTQLLEAELRILASQPPK
                     GRVRPTKIAVMMPSAAKISPAPPAEALAKAAASAAEPAARATVTGGEVPAKALPSAAA
                     ANVTAANVTATNGAAANGAANGATAVNGTAVAAGNPPRNREPERGVWPFDPLAEKPTD
                     RFTLSQKSKEINDIIGGGVGSSLNDKLRPDIVDLRSALNDTPVRDLKKAIGVNDRYVF
                     VNQLFRGDEAMYERSIKTINGFRILPEAEYWMERELKVKLGWDENRDVTRHFYQLVKR
                     RFS"
     gene            complement(2101..>2714)
                     /locus_tag="VKU83_01250"
     CDS             complement(2101..>2714)
                     /locus_tag="VKU83_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92198.1"
                     /translation="VEAFCGNQPVIVAGSTWEEDEEEMDHYANTHPDVRFIVAPHHIG
                     QDRLAEVERLFRHSIRYSDWVKQGPPPLKIRRPGPEPNVLLIDNIGMLSRLYRYATIA
                     FVGGGFGDDGVHNVLEAAVYGKPVLFGPVIEKYVEAVELTECGGGIVIESALEAERVF
                     DRLLRNAAERDEIGRASRDYVQSRKGATERIVRFIQENRLLTS"
BASE COUNT          646 a          812 c          715 g          541 t
ORIGIN      
        1 aggaacagca acggttccgg catcgggttc cccagcatga acacgaacaa tcccaggctg
       61 agcaggctca tcagtaacat gagcagggcg ctgaagagca gcttcgacag gatgaaattc
      121 gccgctccgg tgatcgaata aaaatagagc agccttcccc ggctctcctg caggaagctt
      181 ttggcgaccg cattgacgca aacaaacagc tggatcatcc agaaaaggcc cttccagacc
      241 atcgcttccg gctggcccat tgccaggtac agcacgaaaa tggtcgaggc gacatacagc
      301 aacacgccat agaaactata ttgctgccgg acttccagca acaaatcctt cttaaataaa
      361 atgaatattt gggtgaccga ccggatgccg taaattttgt cgcaaatctc cgcactcggg
      421 gccgataatc aaaaaattcg ttaaaattat tgcgaaccca gcgccggctt caaccgccgg
      481 cctgaaccgc cggcttgaac cgccgacggc ccggcaaaag cggccgggct attccggcag
      541 cccataaaaa cacttgcggc aacgcggctc gtagacgtct ttttcgccca gcagcaattg
      601 aggggaaacc ctggttttcc gaaaggaata gttggcaata tttccacatt ttgcacaaat
      661 ggcatgcaac ttggttatat attctgcttt ggcgagaagg ttaggcatct gcccgaaagg
      721 cttggccctg tagtccatat cgaggccggc gacgattacg cggactccgc gaatggcaag
      781 ctggtcgcag acttcgggca attgatcgtc gaagaactga gcctcgtcta tcccgatcac
      841 ctccacgtcg gccgccatca ggaggatgga ggtggacgtg cttattggcg tggaagaaat
      901 ggcattttca tcgtgagaga ccacgttttc ttcgtggtac cgcgtatcga cggcaggttt
      961 gaagatctcg acccttaagt tggcgatttt ggcacgtttt agcctgcgga tcagctcctc
     1021 ggtctttccg gagaacatgg acccgcaaat aacctcgatc caacctcgtt tctcagccct
     1081 taaatgaggt tcaatgaaca taggcgcagt taaattaagt tcggaatttg ttataacttt
     1141 aaggggacaa ggtcccatgg ctttttcggg ctaacacgac gaaaccgcgg acattaactg
     1201 aattgcggca aaaaatttat gtttttggtc ggttttttga cgcagatgat aggaccacgc
     1261 gaacccatac gactcttaag tacctaacat ggaacgactg aaagaactga tatcccagct
     1321 gacggagcag gttgaccacc aggccgagcc cgccgaaatg ctccagacca cgcagctatt
     1381 agaagccgag ctccgaattc tcgccagcca gccgccgaaa ggcagggtca ggccgacgaa
     1441 gatagcggtt atgatgccat ctgccgcaaa aatcagcccc gcgccgccgg ccgaagcctt
     1501 ggcgaaagcg gccgcctcgg ccgctgagcc cgccgccagg gctactgtaa cgggtggcga
     1561 agtgccggca aaggcgctgc cttcggccgc ggcggcgaat gtcaccgcag ccaatgtcac
     1621 cgcaaccaac ggagcggcag ccaacggagc agccaacggg gcaacagccg tcaacggtac
     1681 cgccgttgcc gccggaaatc cgccgcgcaa ccgtgagccc gaaaggggcg tctggccttt
     1741 cgaccccctt gccgaaaagc cgaccgatcg ctttaccctc tcgcagaagt ccaaggagat
     1801 caatgacatc atcggcggcg gggtcggttc atcactcaat gacaagcttc ggcccgatat
     1861 cgtcgacctg cgctcggcgc ttaacgacac ccccgtccgc gacctcaaga aggccatcgg
     1921 cgtgaatgac agatatgtct ttgtgaacca actatttaga ggggatgagg cgatgtacga
     1981 gcggagcatc aagacgatta acggctttcg catccttccc gaggccgaat actggatgga
     2041 gcgggaatta aaggtcaagc tgggctggga tgagaaccgg gacgtaaccc gccactttta
     2101 tcagctcgtc aagaggcggt tttcctgaat aaagcgcacg atgcgttccg tggcgccctt
     2161 tctcgattgg acgtagtccc gggatgccct gccaatctcg tcgcgctccg ccgcgtttcg
     2221 gagcaggcga tcgaagaccc tttccgcttc cagcgcgctc tcgatgacga taccgccgcc
     2281 gcattccgtc aattccaccg cttcgacata tttttcgatc accggaccaa aaaggaccgg
     2341 cttcccatat accgccgcct cgagaacgtt atgcaccccg tcatcaccga agccgccgcc
     2401 cacaaacgcg atcgtcgcat accggtacag cctcgacaac atcccgatat tatcgatcag
     2461 taagacattc ggctccggtc ccggccgccg aatcttcagc ggcggcggcc cctgtttcac
     2521 ccaatccgaa taccggatcg aatgccggaa tagcctttcc acctccgcca gccggtcctg
     2581 gccgatatgg tgcggcgcca caataaaccg gacgtcggga tgggtgttcg catagtggtc
     2641 catctcctct tcgtcttcct cccaggtgct gcccgccacg atcaccggct gatttccgca
     2701 aaacgcctcg actg
//
LOCUS       DATGKE010000074         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_704225, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000074 DATGKE010000000
VERSION     DATGKE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704225"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1404
                     /locus_tag="VKU83_01255"
     CDS             193..1404
                     /locus_tag="VKU83_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92199.1"
                     /translation="MRPTWRTMRCVLTLVAVGLAGALSSQAQSKTEDSLARQKDLRDI
                     AHSVFNKNYQPEPVRSQAAFHNSPFPAVGYTLVTGIAAVFSDRMDFHTGDSTGKITDI
                     LSSFTYSQYNQIIAQSYADIWAGHDKFNIIADWRYMKYPSTTFGLGGHTQYSDGYTID
                     FSYIKVHTTVLRYVLPNLYAGLGYYYDYFYKIQEVDPPPGVLTSFEKYGLTSMESGSG
                     PVARVLYDSRDNPPNALKGWYASVLYHPSFTALGSQGNWAQLQIDIRKYIKLRKDGRN
                     VLALWNFDWLSVGGKTPYLMLPSTGWDDQFNTGRGYIQGRYRGTDMTYLEVEDRFAIT
                     RNNLFGGVVFANVESFKRNLATSFSEDIPGWGAGLRIKLNKHSNTNICVDYGFGLEGS
                     QGIAVNVGEVF"
     gene            complement(1382..>2371)
                     /locus_tag="VKU83_01260"
     CDS             complement(1382..>2371)
                     /locus_tag="VKU83_01260"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLI92200.1"
                     /translation="PALMAGQETETEAESIDRCFSPEHIRDSQLQMLFAVCTPAIASE
                     AQIGLALRILCGFGIDEIADAFLSNKETINKRLFRAKEKLRAEKVKLELPPAAEIPAR
                     LDNVLHIIYLLFSEGYYSRTQHRILQKELCLEAMRLGVLLTEYDRTDLPKTNALMALM
                     CFHASRFEARLTNETELILYEQQDERLWDNSLIKRGMQYLARSATGDQLSSYHLEARI
                     ASWHCIKEDSGEKWEEILQLYNQLLMINYSPSVALNRTFALYKANGREEALAEAEKLK
                     LEGNHFYYLLLGFLYQDVDDSRCRLHWEKAYELAKTDPERQQILGKIKTLPRH"
BASE COUNT          514 a          640 c          676 g          541 t
ORIGIN      
        1 ctgccgccgg accagttctt ttggggcatt atcgtttacg cggcaaaatg atcgatccat
       61 gggcgggacg acaaagtaag gcggcggaag gcataatgcg gaaggctgat ttttcgttta
      121 atacccggaa cgatatccct aagcatcgag atatgacatg ttttcaagcc cggaaataaa
      181 attcaagacc tgatgaggcc cacatggaga acgatgaggt gcgtgctgac cctggtggcc
      241 gtgggcctcg cgggcgcctt gtcctcgcag gcgcaatcca aaacggagga ttcgcttgct
      301 cgccaaaagg acctgcggga tatcgcgcac agcgtcttca acaaaaatta tcagccggag
      361 cccgtccggt ctcaggcggc atttcacaat tcgccattcc cggcggtggg ttataccctg
      421 gtcaccggga tcgcggcggt tttcagcgac cggatggact ttcataccgg ggattcgacc
      481 ggcaagatca ccgacatcct ttccagcttc acctactcgc aatacaacca gatcatcgcc
      541 cagagttatg ccgatatctg ggccgggcat gacaagttca atatcatcgc tgactggcgc
      601 tatatgaaat atccgtccac aaccttcggc ctgggcggcc atacgcaata ttcggacggc
      661 tataccatcg acttcagtta tatcaaggtc catacgacgg tgctccggta cgtgttgccc
      721 aacctttacg cgggcctggg atattattac gattatttct ataagatcca ggaggtggac
      781 ccgccgcccg gggtgttaac cagttttgaa aagtacgggc tgacttcgat ggaatccggg
      841 agcgggccgg tggcgagggt tctttatgat tcgcgggaca atccgcccaa cgcgctgaaa
      901 ggctggtatg ccagcgtcct gtatcatccc agcttcacgg ccctgggaag ccagggaaac
      961 tgggcgcagt tgcagatcga catccgcaaa tacatcaaac tgcgcaaaga cggccggaat
     1021 gtgctggcgc tctggaattt cgactggttg agcgtcggcg gcaaaacgcc gtacctgatg
     1081 ctgcccagca cggggtggga tgaccagttc aataccggcc ggggttatat ccagggaagg
     1141 taccggggca cggatatgac ctacctggag gtggaagacc ggtttgcgat cacccggaac
     1201 aacctgttcg gcggcgttgt atttgctaat gtcgagtcct tcaagcggaa cctggccacc
     1261 tccttcagcg aggacattcc cggctggggg gccggcctcc gcatcaagct caacaaacac
     1321 tccaatacga atatctgtgt cgactacggg tttgggttgg agggctcgca gggcatcgcg
     1381 gtcaatgtcg gggaagtgtt ttgatctttc caaggatctg ctgccgttcc ggatcagtct
     1441 tcgccagctc ataggctttt tcccaatgga gcctgcacct tgagtcatca acgtcctgat
     1501 acaaaaagcc cagcaacagg taataaaaat ggtttccttc cagcttcagc ttctctgctt
     1561 cggcgagggc ttcttccctg ccgttcgcct tgtagagggc aaacgtccgg ttaagggcga
     1621 cgctgggcga ataattgatc atcaggagct ggttatagag ctgcaggatc tcttcccatt
     1681 tttcgcccga atcttctttg atacaatgcc atgaagcaat cctggcttcg aggtgatacg
     1741 agctcaactg atctccggtc gcggagcggg caaggtattg catcccgcgt ttgatgagcg
     1801 aattgtccca aagacgctcg tcctgctgct cgtacaggat gagctcggtt tcgtttgtca
     1861 ggcgtgcttc gaaccgggag gcatgaaagc acatcagggc catcagggcg ttggtcttcg
     1921 ggagatccgt gcggtcgtac tccgtgagca gcacgccgag ccgcatggct tccaggcaaa
     1981 gctctttctg caggatgcga tgctgggtcc tggaataata tccttcgctg aaaagcaggt
     2041 aaatgatatg caggacattg tccagcctgg caggaatttc cgccgcgggc gggagctcca
     2101 gtttaacctt ctcggcccgt aatttctcct tcgcgcggaa gaggcgtttg ttgatggtct
     2161 ctttattgga aaggaatgcg tcagcgatct catcgatccc aaacccgcag aggatccgaa
     2221 gggccaggcc aatttgtgct tcgctggcaa tggccggcgt gcaaacggca aagagcattt
     2281 gcagctggct gtcgcggata tgctccgggg agaaacatcg atcgatgctt tccgcctccg
     2341 tttccgtttc ctggcccgcc attagcgccg g
//
LOCUS       DATGKE010000075         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_704094, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000075 DATGKE010000000
VERSION     DATGKE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704094"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VKU83_01265"
     CDS             complement(<1..607)
                     /locus_tag="VKU83_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92201.1"
                     /translation="MQQVSKIKVLLAEDDLSLGYVIKDNLVDAGYDVTLCPDGQSAID
                     KFDKNEFGICLLDVMMPNKDGFAVAKKIRQQSDMVPILFLTARSMEEDKIKGFQSGAD
                     DYITKPFSMQELLMRMEVFLRRTRKMHSEKSVTFTIGQLNFSYTDLKLTIGQEVFNLT
                     QKEADLLKFLCEHSNRILKREEVLLNVWGKDDYFLGRSMDVF"
     gene            complement(617..1897)
                     /locus_tag="VKU83_01270"
     CDS             complement(617..1897)
                     /locus_tag="VKU83_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI92202.1"
                     /translation="MRSTTLKWIVLSATLLAAIVVTVQLYWLKKVYSFEQRSFNTNVV
                     KSIRSLFEDLQMNDAPGTHLESLIKAPDMDNDYYVFKADTIPIKDSLVYYIQDEFADF
                     GVLTDCKLGAYSSTEKKYIYEEYIPAPASRYGSVQTMTSELPVFYNSFDHILLYFPHR
                     HEYILEQMDFWIVSSVVLFIALIGLAASLFYFYRQKFLAEIQKDFVNNFTHEFKTPLA
                     VMKIASDVLSQDSIASQPGRLVRYAAIIQSQTEHLQNQVERLLRTASIDTKDLTLRKE
                     TVEADLLIEEALSKVQPLIEQKKAKVEVKMEAPVGRVQADKAHLELALVNLLENALKY
                     SPDPHIVVQAGQDEDDFFISVKDNGVGIEKQYLKNIFKKFYRVPTGDIHNVKGFGLGL
                     NFVKRIIDAHKGKIRVNSLPGIGTEFRLFIPLNN"
     gene            2143..2349
                     /locus_tag="VKU83_01275"
     CDS             2143..2349
                     /locus_tag="VKU83_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92203.1"
                     /translation="MKKLLLTGIVLLTITGFAVAQDHHPVHHTHHVVHHSVHHPVHHS
                     VHHSLHHSGYHTVHHRPVHRPERH"
     gene            complement(2412..>3019)
                     /gene="secF"
                     /locus_tag="VKU83_01280"
     CDS             complement(2412..>3019)
                     /gene="secF"
                     /locus_tag="VKU83_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLI92204.1"
                     /translation="QLEAGAVKATIFALIIIFLYIFVRFRDWRYSLGTIISLLHDVFV
                     TLTVFSFAGSFVHFSLEIDEQFIAAVLTVIGFSMNDTVIVFDRIRENSHLMKNATNGE
                     IINKSVNDTLSRTIMTSLTVFLTILILFLVGGEVTRGFAFAMLVGVITGTYSSIFVAA
                     PFLVDFAKGKPLGEKPLAAGKPGAGAEKAAKAAAAAAAAHK"
BASE COUNT          691 a          751 c          799 g          778 t
ORIGIN      
        1 tgaagacgtc catgcttctt ccgagaaaat agtcatcctt tccccacaca ttcaacagga
       61 cttcctccct tttcaggatg cggttggaat gttcgcaaag gaatttaagg agatcggcct
      121 ctttctgggt caggttgaac acttcctgac cgatggtcag tttcaggtcg gtatatgaaa
      181 aattgagttg cccgatggta aacgtaacgg atttctcgga atgcattttc ctcgtccttc
      241 gaaggaatac ctccatgcgc atcagcaact cctgcatgga aaacggcttg gtgatgtagt
      301 cgtcggcgcc gctctggaag ccctttatct tgtcttcttc cattgaccgg gcggtcagga
      361 agaggatggg caccatgtcg ctttgctggc gaattttttt ggcgaccgca aacccgtcct
      421 tgttaggcat catgacgtcc agcaggcaaa tgccgaattc attcttgtcg aacttgtcga
      481 ttgcggattg tccatccggg cagagcgtca cgtcatagcc cgcatcgacc aggttgtcct
      541 tgatgacata gccgagcgac aggtcgtctt ctgccagcag cactttgatt ttcgatactt
      601 gttgcatagc gggtgattag ttgttaaggg gtataaatag cctgaattcc gtcccgatgc
      661 ccggcaggct gttcactctg atctttcctt tatgggcgtc gatgatgcgt ttgacgaagt
      721 tcagtccgag gccgaatccc ttgacgttat gaatgtctcc ggtcggaacc cggtagaact
      781 tcttaaagat attcttgagg tattgttttt cgataccgac gccattgtct ttcaccgaaa
      841 tgaaaaaatc atcttcatcc tggccggcct ggaccacgat atgggggtcg ggcgaatact
      901 taagcgcatt ttccagcagg tttacaaggg caagttccag gtgggcttta tccgcctgaa
      961 cgcggccgac cggggcttcc atttttactt ccacctttgc ctttttctgt tcgatcagtg
     1021 gttgcacctt gcttagcgct tcttcgatca gcaggtcggc ttccacggtt tcctttcgaa
     1081 gggtcaggtc cttggtgtcg atcgatgcgg tccgcagcag gcgttccacc tgattctgga
     1141 gatgctcggt ctggctctgg atgatcgcgg cataacgcac cagccggccg ggttggctgg
     1201 caatggaatc ctgcgacagt acgtcggatg cgatcttcat gacagcaagc ggcgtcttga
     1261 actcgtgcgt gaaattgttg acaaagtcct tttggatctc ggcgaggaat ttctgccggt
     1321 aaaagtaaaa aaggcttgcg gcaagaccga tgagggcaat gaagaggacg acggaactga
     1381 cgatccagaa atccatttgc tcgagaatgt attcgtggcg atgcgggaaa tagagcagga
     1441 tatggtcgaa gctattgtaa aatacaggca gttccgaggt catcgtctgc acgctgccgt
     1501 aacgggaggc aggcgccggg atatattcct cgtaaatgta tttcttttca gtgctgctat
     1561 aggcgcccag cttgcagtcc gtcaggacgc cgaaatcggc gaattcgtcc tggatatagt
     1621 aaacgaggga gtctttaata ggtatggtgt cggccttaaa aacataataa tcgttgtcca
     1681 tatccggcgc cttgatcagg gactcaagat gggtcccggg agcgtcgttc atctgcaggt
     1741 cttcgaaaag gctgcgaatg cttttgacga catttgtatt gaaagagcgc tgttcaaacg
     1801 aatagacctt tttcagccag tacaactgca ccgtcacgac aatagccgcc aacagggtgg
     1861 cagacaggac gatccatttc aaggtggttg aacgcatgga tgcaaaattg atcctcttaa
     1921 cgctttttgg gtgatgaatc tggttaagtg ctggttaaga gctagataca aaattcgggt
     1981 ttttttctca tgactttcgt tccggtagac ccgtttatag gtatatattt ttctgattga
     2041 cagtgagtta ccgggaggta gcgcctggag ccggggcgat gccggaatca ggcatttttt
     2101 ttgatcctac cgctggtaat atcttatcac aaaaaattca tcatgaagaa gctcttattg
     2161 accgggatcg tcctgcttac cattaccggg tttgccgtgg cgcaggatca ccatccggtg
     2221 caccacaccc accatgtcgt acatcattcg gtgcatcacc ccgtgcatca ttcggtacac
     2281 cattcgctgc atcattcggg ataccatacc gtacatcacc gtccggtcca ccgaccagaa
     2341 cgtcattaaa ctaaagaccc cgggcaagcc gcccggggtc ttcttttcac tctaaaaaaa
     2401 gttcgttcca actatttatg tgctgctgct gcggcggcgg cggcttttgc ggctttttcc
     2461 gcacctgctc cgggctttcc ggcagcaaga ggtttctcac ccagcggctt gcccttggca
     2521 aagtcgacca ggaatggcgc ggccacgaag atcgaagaat atgttccggt gatcaccccg
     2581 accagcattg caaatgcgaa tccgcgggtc acttcaccgc ctaccaggaa aaggatcagg
     2641 atggtgagga acacggtcag ggaggtcatg atcgtccggg aaagggtgtc gttgaccgac
     2701 ttattgatga tttccccgtt cgtggcattc ttcatcaggt ggctattctc ccggatccgg
     2761 tcgaagacga tcaccgtatc gttcatggag aagccgatca ccgtcagtac cgcagcgatg
     2821 aactgctcat ctatctccag tgagaagtgc acaaaacttc cggcgaatga aaacacggtc
     2881 agcgtgacga agacgtcgtg gagcagggag atgatcgtac ccagtgaata gcgccagtcg
     2941 cggaaccgga cgaagatata gaggaaaatg atgatcaggg caaagatggt cgccttgact
     3001 gcacccgcct ccagctgtt
//
LOCUS       DATGKE010000076         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1448538, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000076 DATGKE010000000
VERSION     DATGKE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448538"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2350)
                     /locus_tag="VKU83_01285"
     CDS             complement(<1..2350)
                     /locus_tag="VKU83_01285"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92205.1"
                     /translation="MAKYLFLWMISVVLFATRSYSQSRGTVKGVLVDTSGGRQPLANA
                     TISVTPVPGDSTDAEYTVSDKNGNFSMRGIRSGHYKLLITYEGYVPVEKRFLVADTGG
                     VINFALLYMQRADNTLEAVVIQRPPMGIKKDTVEYNASSFAVKPNATAEDLLNKMPGI
                     TVDHSGTVTAHGETVQRVLVDGKRFFSDDPKLATRNLPPDIIDKIQVFDDLSDQSKFT
                     GFDDGNRVKTINIVTKKNARKGYFGKLVAGAGTDQAYDESINLHRFDGNQQISLLGQA
                     NDINKQNFSPQDIFGGNGGGGGGRRGGGGGGPAASTSYQSNGVTTVWAGGANYRNTFG
                     TNPNHSTDVYGSYFYNNQHVVLKQTDSAISPVPNDLGHDSSLVTSGGNTAISRIENHR
                     VFLNIESRLDTNNSLIFRPNITFQRSTPDGSSFSSEYDNHGNLINSLNGHTSSENTGF
                     SINNSNLQFRHRFAKPYRTISLDVSTTANVNNGTGYTYSVNQFYTGLNRVDTINQFYR
                     DSLHSWTVTPTLSYTEPIAKNQILQFSYSHTYNTSTSINSTFDYVDSLKGYSRFDYLF
                     SNSYKFRSNADNAGLYWRTQNAKYTLSIGSGIQFTNFTSINTTKNNLTVSRNYVNYTP
                     SAIFNYSFSNTSHLRIFYQGRTGTPSASQLQPLTTTSDSVNFQLGNPALKPQFVHSLR
                     LLYANFDPASQHVIFATVNASTTVNDIQNVSYYNKEGGKNSTYANLNGTWNVSGFFNY
                     GFPLKRPKSNLNFMTNVSYTQSQTGLATDSAAYLAGQYAHLYT"
     gene            complement(2515..>3189)
                     /locus_tag="VKU83_01290"
     CDS             complement(2515..>3189)
                     /locus_tag="VKU83_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HLI92206.1"
                     /translation="IYDAYRNDYRDKIMGTIETQGIQRSQLTILQGLMKLRQICDSPA
                     ILNETDKYPNHSIKLDELAREITENIGDHKALIFSQFLGMLALIRERLRELDIKFEYF
                     DGSTSAQERERAIQSFQNDDTCRVFLISLKAGGVGLNLTAADYVYIVDPWWNPAVEQQ
                     AIDRTHRIGQTKNIFAYRMICKDTIEDKILQLQEKKRVLAKDLIADDDGFVKSLTRAD
                     VEYLFS"
BASE COUNT          673 a          764 c          950 g          802 t
ORIGIN      
        1 tggtatacag gtgcgcatac tggccggcaa gataagcggc ggaatcggta gccagccccg
       61 tttggctttg cgtatagctg acattcgtca tgaagttcag gttggatttg gggcgtttca
      121 gcgggaagcc gtagttgaag aaaccgctca cattccaggt gccgttcagg ttggcatacg
      181 tactgttctt cccgccctcc ttgttgtagt aggaaacatt ctggatatcg ttcaccgtag
      241 tgctggcatt cacggtagca aagatcacgt gctgcgatgc cggatcgaaa ttcgcataca
      301 ggaggcgcag ggaatgcacg aattgcggct tgagcgccgg attacccagc tgaaaattga
      361 cgctgtcgga agtagttgtg agtggctgca gctgggacgc ggagggtgta cccgtacggc
      421 cctggtagaa tatgcggagg tggctggtat tggagaaact atagttaaaa attgccgaag
      481 gggtatagtt cacgtagttg cgcgatacag taagattatt cttggtggta ttgatgctgg
      541 taaaattcgt gaattggata ccggacccga tagacaaggt gtacttggca ttctgggtgc
      601 gccaatacag accggcgttg tcggcattgg agcggaactt atagctattg ctgaacaggt
      661 agtcgaaacg cgaatagcct ttcagcgaat ccacataatc aaaggtactg ttgatcgagg
      721 tgctcgtatt ataggtatgg ctgtacgaga attgcaggat ctggttcttg gcgatgggtt
      781 cggtataaga cagggtcggg gtgacagtcc acgaatgcag agagtcccgg tagaactgat
      841 tgatggtgtc gacgcggtta aggccggtat agaactgatt caccgaatag gtatatcccg
      901 tgccgttgtt gacattggcg gtcgtactca cgtctagcga gatcgtgcga tagggtttgg
      961 cgaaccggtg ccggaactgg aggttcgagt tgttgatact gaagccggta ttctcgctgg
     1021 aggtatgccc gttgagactg ttgatcagat tgccgtgatt atcgtattcc gaggaaaaag
     1081 aagatccgtc gggtgtgctt cgctggaaag tgatattcgg gcgaaagatc agtgagttat
     1141 tggtatccag gcggctctcg atgttcagaa agacacggtg gttctcgata cggctgatcg
     1201 ccgtattgcc gccgctggtg accaggctgg agtcgtggcc caggtcgttg gggaccgggg
     1261 agatcgcact atccgtttgt ttgaggacga cgtgctggtt gttatagaaa tagctcccgt
     1321 aaacatccgt cgaatggttg ggattggtcc cgaacgtatt ccggtagttc gcgccaccgg
     1381 cccaaaccgt agtcacccca ttggactgat agctggtgct ggccgctggg ccaccaccgc
     1441 cgccgccccg gcgaccgcca ccgccgccgc cattgccacc gaagatatcc tggggactaa
     1501 agttctgttt gttgatgtca ttggcctgcc ccaacaggga gatctgttgg ttcccatcga
     1561 aacggtgcag gttgatgctt tcgtcatagg cctgatcggt gccggcgcca gccaccagct
     1621 ttccgaaata acctttccgg gcgttctttt tggtcacgat attaatggtc ttcacccggt
     1681 tgccgtcgtc gaagccggtg aacttgcttt ggtcgctaag gtcgtcgaag acctggatct
     1741 tatcgatgat atccgggggc aggttacggg ttgcaagctt agggtcgtcg ctgaagaagc
     1801 gtttgccgtc cacaaggacg cgctggacgg tctcaccatg cgctgtaaca gtaccgctat
     1861 gatccacggt gataccgggc atcttattga gcaggtcctc cgccgtagcg ttgggtttca
     1921 ccgcaaaaga gctggcattg tattctaccg tatccttttt tatccccatg ggtgggcgct
     1981 ggatgacgac ggcctccagg gtattgtcgg cccgctgcat atagagaagg gcgaaattga
     2041 tcacgccgcc ggtgtccgct acgaggaatc gtttttcgac cggtacatag ccttcgtagg
     2101 taatgagcag cttataatgg ccggaccgga tgcctctcat ggaaaaattg ccgttcttat
     2161 ccgaaacggt gtattccgcg tcggtggaat cgcctggcac gggggttacc gaaatagtag
     2221 cgttggcgag cggctgccgc cctcccgaag tgtcgacaag gactcccttc acggtgcctc
     2281 tcgactgtga atatgaacgt gttgcgaata aaaccacgct aatcatccat aagaacaggt
     2341 acttagccat atgcttgttt gtcttgcaaa atctctgcta aattagcctt ttgggcggga
     2401 gcaggcatac cgtcccggta ccagtggtaa atttaggttg ccagtagggt gaaccggcgg
     2461 gtaaatgcca accgtgatcg gtgaatattg gccgcgagcc gacggacgtt ctggctagga
     2521 gaacaggtat tccacatcag ccctggtaag gctctttacg aacccgtcgt catcggcgat
     2581 gaggtctttt gccaggacgc gttttttctc ctgcagctgc aggatcttat cttcgatggt
     2641 atccttacag atcatgcggt aagcgaagat gttcttggtc tggccgatcc ggtgggtgcg
     2701 gtcgatcgcc tgctgctcta ctgcagggtt ccaccagggg tcgacaatgt agacatagtc
     2761 ggccgctgta aggttcagac ccacgccgcc cgccttgagc gagatcagga acacgcggca
     2821 ggtatcgtcg ttctggaagc tttggatagc ccgttcgcgt tcctgcgcag aagtgctgcc
     2881 gtcgaagtat tcgaatttga tatccagctc cctgagtctt tcccggataa gcgccagcat
     2941 gcccaggaac tgggagaaga tgagcgcctt atggtctccg atgttctcgg tgatctcccg
     3001 ggcgagctca tccagcttta tcgaatgatt gggatatttg tccgtttcat tgaggatggc
     3061 cggagagtcg cagatctgcc gcagtttcat cagaccctgc aaaatggtga gctgcgatcg
     3121 ttggataccc tgtgtttcga tggtccccat gatcttgtcc cgatagtcat tgcggtatgc
     3181 atcatagat
//
LOCUS       DATGKE010000077         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1443303, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000077 DATGKE010000000
VERSION     DATGKE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443303"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VKU83_01295"
     CDS             <1..690
                     /locus_tag="VKU83_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLI92207.1"
                     /translation="ARDRIAGFRYQGPFTPASYEGTIMAPGNVGGIHWGGMCYDPGTG
                     MLFTNINRVAALIRMIPRGKLDSVVLSSNEVLRAETGRQQGTPYVMKRDYLFRQAGNG
                     FLMQTRPPWGTLVGIDLRTGGKKWEVPLGYMMDPDKYPDARRWGSLNFGGAIVTNGGL
                     VFIAAGIDTHFRAFDSRTGKVLWEYALPASAQATPMTYALDGRQYVIICAGGHGKLHT
                     KQGDYVMAFAL"
     gene            790..1026
                     /gene="rpmB"
                     /locus_tag="VKU83_01300"
     CDS             790..1026
                     /gene="rpmB"
                     /locus_tag="VKU83_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLI92208.1"
                     /translation="MARVCQITGKVPVTGHKVSHSNIKTKRRFLPNLQTKRFFLAEED
                     KWITLKVSSEGLRTINKNGLYNVVKQLRAQGEKI"
     gene            1063..1245
                     /gene="rpmG"
                     /locus_tag="VKU83_01305"
     CDS             1063..1245
                     /gene="rpmG"
                     /locus_tag="VKU83_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLI92209.1"
                     /translation="MAKKGSRVQVILECTEHKASGQPGTSRYITTKNKKNTPERIELK
                     KYNPILKKVTVHKEIK"
     gene            1248..1421
                     /locus_tag="VKU83_01310"
     CDS             1248..1421
                     /locus_tag="VKU83_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HLI92210.1"
                     /translation="MAKAAKTAIKTKDAKAAAEAKNWTKVIRAVRSPKTGAYTFKETI
                     VHKDKIKQFLAQK"
     gene            1481..2443
                     /gene="ftsY"
                     /locus_tag="VKU83_01315"
     CDS             1481..2443
                     /gene="ftsY"
                     /locus_tag="VKU83_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLI92211.1"
                     /translation="MGLFDKLFGKKEKESLDQGLQKTKEGFISRLTKAIAGKSTVDEE
                     VLDHLEDALVSADVGVDTTVQIIRQIEQRVAKDKYVNTSELNKILQQEIEQMLVDAPE
                     QSYRDFSLPAAGRPYVILVVGVNGVGKTTTIGKLAYHFKRAGKSVLLGAADTFRAAAV
                     DQLTIWSERVGVPIIKQGMGSDPGAVAFDTVQSGIARGADVVIIDTAGRLHNKAFLME
                     ELTKISRVIKKLMPDAPHEVLLVLDGSTGQNALEQARQFTAATNVTALAITKLDGTAK
                     GGIVLAIANQFRIPVKFIGVGEKMEDLLVFDKHEFVDSLFSLEK"
     gene            2440..3759
                     /gene="rimO"
                     /locus_tag="VKU83_01320"
     CDS             2440..3759
                     /gene="rimO"
                     /locus_tag="VKU83_01320"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HLI92212.1"
                     /translation="MKTRTLKKDKVNIITLGCSKNMVDSEVLSGQLRANDIDVVHENT
                     RTDHNIVVVNTCGFIDKAKEESVNTILDQVELKKRGRLDKVYVTGRLSQRYREDLEKE
                     IPEVDAWFGTLELPLMLRQFEADYKVELLGERLLATPKHYAYLKISEGCNRTCAFCAI
                     PLMRGGHISRPVEDLVKEAEALVRKGVKEILLIAQELTYYGLDLYKRRALPDLLNRLA
                     DIPGLEWIRLHYAYPAKFPVEILDVIRERPNICNYLDMPLQHASDRMLNAMRRQITRQ
                     GMKELIQTVREKVPGICLRTTLIAGFPGETEDDICELKDFLREHRFDRVGVFTYSHEE
                     GTSAYGLEDNVPAAVKAQRAQELMEVQQEISYEINQQKIGRTFKVLIDKKEAGRYLGR
                     TEFDSVEVDNEVVVYSKNTLPIGEFTHVRITTAYDYDLEGDALPAES"
     gene            3833..4747
                     /locus_tag="VKU83_01325"
     CDS             3833..4747
                     /locus_tag="VKU83_01325"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLI92213.1"
                     /translation="MRLNWNGVYPALTTKFTAKESLDLEAFGANLDAQVQAGVNGVIV
                     GGSLGEASTIALEEKETLVKYCVSHTSLPVLLNIAEGSTREAMNQAALASRWGASGLM
                     LLPPMRYKSDHRETVSFFKSVAAATDLPVMIYNNPIDYKIEVTLEMFDELTELSNIEA
                     VKESTRDITNVTRLINRFGERLKILCGVDPIAMEALLMGAHGWVAGLVCAFPAETVAI
                     YRLVKAGRIEEALEIHRWFLPLLELDIHSKLVQYIKLAEAEAGLGTEYVRAPRLPLIG
                     EERERVLAIIRRGLATRPVLPEYLSLAV"
     gene            4769..>5096
                     /locus_tag="VKU83_01330"
     CDS             4769..>5096
                     /locus_tag="VKU83_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLI92214.1"
                     /translation="MTFRDATAAEIDVVMEAAWQAFPVFREKTLKERADLMRAIAKEL
                     EATGDELIQVAMRETHLPEARLRNERARTQFQLTSYADACASGDWLEVRIDTAQPGQG
                     APDLRKW"
BASE COUNT         1285 a         1382 c         1445 g          984 t
ORIGIN      
        1 gcccgcgatc gtatcgcggg gtttcgttac caggggcctt ttacacctgc gagttatgaa
       61 gggaccatca tggcgccagg taacgttggc gggatccatt ggggtgggat gtgctacgat
      121 ccgggcaccg gtatgctttt caccaatatc aatcgtgtag cggcgctgat ccggatgatc
      181 ccgcgcggta agctcgacag cgtcgttcta tccagcaacg aagtgctccg cgccgagaca
      241 gggcgccagc agggcacgcc atatgtcatg aagcgggact acctgttcag gcaggccggc
      301 aatggttttc tgatgcaaac ccggccgccc tggggcacgc ttgttggtat cgatctccgg
      361 actggcggga agaaatggga agttccactc gggtatatga tggacccgga caaataccct
      421 gatgcccggc gatggggctc cctcaatttc gggggtgcta ttgtaacgaa cggcggtctg
      481 gttttcattg cggcaggcat cgatacgcat ttccgcgcct tcgatagccg gacaggaaaa
      541 gtcctctggg aatatgccct gcccgccagc gcacaggcca cgcctatgac ctacgcgctc
      601 gacggccggc aatatgtcat catttgcgcc ggcggccacg gcaagttgca tacgaagcag
      661 ggggattacg tgatggcgtt cgccctgtga cctgcctcgg ggacccgggc cggatcagac
      721 cgaaaaaacc agaattcttt tggttaattg ccggtatttc acgacctttg cagtccaaat
      781 tttctcatca tggccagagt atgtcagatt acgggtaaag ttccggtcac cggtcacaag
      841 gtgtcgcact cgaacatcaa gacgaaacgc cggttcctgc ccaatttgca gaccaaacgc
      901 ttcttccttg ccgaggaaga taagtggatt accctgaagg tctcctccga agggctgcgg
      961 accatcaaca aaaacggtct gtacaatgta gtaaagcaac tgagagcaca gggagaaaaa
     1021 atctaaaatg cggccggcag aaccggcata aaacatccaa caatggcaaa gaaaggcagc
     1081 agggttcagg tcattctgga atgcacggag cataaggcca gcgggcagcc cggaaccagt
     1141 cgttatatca cgaccaagaa caaaaagaac accccggagc ggatagaatt aaagaaatac
     1201 aatccgatcc tgaaaaaagt aaccgttcac aaagaaatta aataaccatg gcaaaggccg
     1261 cgaaaaccgc aatcaagacc aaggacgcca aagcagccgc tgaagcgaag aactggacaa
     1321 aggtcatccg ggccgtacgc agcccgaaga ccggcgccta tacgtttaag gaaacgatcg
     1381 tccacaagga caagatcaag cagtttttgg ctcaaaaata gttttgggtt aactaagata
     1441 ttaaaagctg cccccgggca gctttttcaa ttggagcgat atgggactgt tcgacaaact
     1501 cttcggaaaa aaggaaaagg aaagcctcga ccagggcctt caaaaaacaa aagaaggatt
     1561 catatcccgg ctcaccaaag cgatcgcagg aaaaagcacc gttgacgaag aagtgctcga
     1621 ccatcttgag gatgccttgg tcagcgccga tgtcggcgtc gataccaccg tccagatcat
     1681 ccgccagatc gagcagcggg tagcaaagga caagtatgtc aataccagtg aattaaataa
     1741 gatacttcaa caagaaattg agcaaatgct ggtggatgcc ccggaacaat cgtaccggga
     1801 tttcagtcta ccggccgctg gcaggcccta tgtcatcctc gtggtcggcg tcaatggcgt
     1861 aggcaagacc accaccatcg gaaaactggc ctatcacttt aagcgggccg gaaaatccgt
     1921 ccttctggga gcggcggaca ctttccgggc ggcagcggtc gaccagctta ccatctggag
     1981 tgagcgggtt ggggttccga taatcaaaca gggcatgggg tcggatccgg gcgccgttgc
     2041 ttttgacact gtccaaagcg gcatcgcgcg cggcgcagac gtcgtcatca tcgacaccgc
     2101 cgggcgactt cataacaaag cttttctcat ggaagaactg accaagatca gtcgcgtgat
     2161 caagaagctc atgcccgatg ccccccacga agtgctcctc gtgctcgacg ggtcaaccgg
     2221 ccaaaacgca ttggagcagg ctcgccagtt tactgccgca acaaacgtca cggcactggc
     2281 gatcacgaaa ctggacggca ccgccaaagg cgggatcgta ctggcgattg ccaaccagtt
     2341 caggatccct gtgaagttta tcggggtcgg cgagaaaatg gaagacctgc tggtcttcga
     2401 taaacatgaa tttgtggaca gccttttcag ccttgaaaaa tgaaaacccg cacgcttaag
     2461 aaggacaagg tcaatatcat caccctgggg tgcagcaaga atatggtcga cagcgaggtt
     2521 ctgagcggcc agcttcgggc caacgatatc gacgtcgtgc acgagaacac ccggacggac
     2581 cacaatatcg ttgttgtcaa cacctgcggt tttatcgaca aggccaaaga ggagtctgtg
     2641 aataccatcc ttgaccaggt tgagctcaaa aagcgcggcc ggcttgacaa agtttatgtg
     2701 acaggccgcc tcagccagcg ctaccgggag gatctcgaaa aagagatccc tgaagtcgat
     2761 gcgtggttcg gcaccctcga gttaccgctg atgcttcgtc aatttgaggc ggattacaag
     2821 gtcgaattgc tcggcgagcg gttgctggca accccaaaac actatgccta cctgaagatc
     2881 tccgagggct gcaaccgcac ctgcgctttt tgcgccatcc cgctgatgcg cgggggccat
     2941 atcagccgcc cggttgagga ccttgtaaag gaggcggaag ccctggtccg taaaggggta
     3001 aaggaaatcc tgctcattgc ccaggaacta acctattatg gcctcgacct atacaaacgc
     3061 cgggcactcc cggacctgct caaccggctg gcggacatcc cgggcctgga atggatcagg
     3121 ctgcattatg cgtatccggc aaaattcccg gttgaaatcc tcgacgtgat ccgggagcgg
     3181 ccgaatatct gcaattacct agatatgccg ctccagcatg ccagcgaccg gatgctgaac
     3241 gccatgcggc gccagatcac gcgccagggt atgaaggaac tgatccaaac cgtccgcgaa
     3301 aaggtgcccg ggatctgtct tcggaccacc ctcatcgcgg gtttcccggg ggaaaccgaa
     3361 gacgatatct gcgaattgaa ggattttctc cgggaacacc ggttcgaccg ggtgggcgtc
     3421 ttcacttatt ctcacgagga aggcacgtcc gcgtacggct tggaagacaa tgtccccgcc
     3481 gcagtcaaag ctcaacgggc acaggaactc atggaggtcc aacaggagat ctcttacgag
     3541 atcaaccaac aaaagattgg aaggacgttc aaggtgctga tcgacaaaaa ggaggcgggc
     3601 cgttatcttg gccggacgga attcgattcg gtagaggtgg acaacgaagt agtcgtctac
     3661 agcaaaaata cattgccgat cggcgaattc acccatgtcc ggatcacgac ggcgtatgat
     3721 tacgacctgg agggagacgc gctgccggcc gaaagttaaa atccggtaat aaaaagtcaa
     3781 ttgttgcggg cctggcggcc cggttaagcg atatatttgt aaaaaacgga acatgagact
     3841 gaactggaac ggcgtttatc ccgcactcac aacgaaattt accgcgaagg agtccctcga
     3901 cctggaggca ttcggggcca atctcgatgc ccaggtgcag gcgggcgtga acggcgttat
     3961 cgttggcggc tcgctcggcg aagccagcac cattgccctc gaagaaaagg agacgctcgt
     4021 aaaatactgt gtatcccata caagtctgcc tgttctgctg aatatcgccg aaggaagtac
     4081 tcgcgaggcc atgaaccagg ccgccctggc gtccaggtgg ggggccagcg ggctcatgct
     4141 gctcccgccg atgcgctata agagcgacca ccgggagacc gtcagctttt tcaaatcggt
     4201 cgcagccgct accgacctgc cggtaatgat ctataataac cccatcgact acaagatcga
     4261 ggtcaccctc gagatgttcg acgaactgac cgagctttcc aatatcgagg ctgtaaagga
     4321 atcgacgcgt gatattacca acgtcacgcg cctgatcaac cgctttggcg agcggctgaa
     4381 gatactttgc ggtgtcgacc ccatcgccat ggaagcactg ctgatgggag cgcatggctg
     4441 ggtagcgggg cttgtttgcg cttttccggc tgaaaccgtc gcgatttacc gcctcgttaa
     4501 ggcgggacgc atcgaggaag ctttggagat ccaccggtgg ttcctacctt tgctggaact
     4561 ggacatccac tctaagctgg tccaatacat caagttggcc gaggcggagg ccggactcgg
     4621 tacggaatat gtacgggcgc cccggttgcc cctgatcgga gaagaaaggg aacgcgtcct
     4681 ggccatcatc cggagaggac tggcgacacg gcctgtcctg cctgaatacc tgtccctggc
     4741 cgtttaaagt aaattaaaat attgtcttat gacctttcgt gacgcaaccg ccgccgaaat
     4801 cgatgttgtt atggaagccg cgtggcaggc ctttccggtc ttccgggaaa agacgttgaa
     4861 agagcgcgcg gacctcatgc gcgccattgc caaagaactg gaagctaccg gtgacgagct
     4921 gatccaggtc gccatgcggg aaacgcatct gccggaagcc cgccttcgta acgaacgggc
     4981 gcgcactcag ttccagctga caagctatgc ggatgcctgc gcgtcgggcg actggctcga
     5041 agtgaggatc gacacggccc aacctggtca gggggcgccc gacctgcgca aatggt
//
LOCUS       DATGKE010000078         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_290506, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000078 DATGKE010000000
VERSION     DATGKE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290506"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VKU83_01335"
     CDS             <1..207
                     /locus_tag="VKU83_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92215.1"
                     /translation="GIIGGAIGPLIIGGIGDALGLRTGMFFLYVTLGYILSIGFWARP
                     LITNQTVFNKREKAAYETVESDRD"
     gene            179..1030
                     /locus_tag="VKU83_01340"
     CDS             179..1030
                     /locus_tag="VKU83_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLI92216.1"
                     /translation="MRQSKVIGIDLGATNIRGAVVSGDDLSDIASVRIHSNGEVDEVL
                     GDIYSIIDTLMGPGVAGIGIGVPSVVDVDEGIVYDVQYIPSWVEVPLKRLLEARYHKP
                     VYVNNDANCFALGEYYFGKGKGVSSMVGLTIGTGLGGGIIIHNKLFAGSNCGAGEIGC
                     LPYKDKILEYYCSGSFFKNVYDLDGVAVYEAARKGEARALQLYAELGQHVGQAIKMVM
                     YTYDPQLIVLGGSVSQAYDLFKETMWKEVRTLVYAKSLERIRVEVSNLKNSGILGAAA
                     LYYDAKQ"
     gene            1014..>2657
                     /locus_tag="VKU83_01345"
     CDS             1014..>2657
                     /locus_tag="VKU83_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLI92217.1"
                     /translation="MMQNNNFYRAGLAAAFALLVVRAFPQTAGHFSMTLKDDWQMQSS
                     TRVPDQGAAISEAGYMPKGWYKVSVPTTIIAGLLANKVYDFDPFFGMNLEKLKDPTLD
                     LPWWFRKEFTLPATQKGKDVVLSLHGINYKANVWLNGVLIADSNQIMNPYRIIELNVT
                     PYIRETGANTLALEIRRPFNPQKRGGDLAIDYADWIHYPPDYNGGIVNDIVIKTYDRV
                     GIEYPLVTTHFDLPSLDVAHLTVDALVKNYSAGPQDVLVKGRIDTSQDGGGAGITFSK
                     KVHLEANDVDDVVFTPAEFPELNVRHPRIWWPWQYGKPELNRLELSVVRDDRVSNTIA
                     ENFGIREITSEMIDTSSRVFVVNGKRILIRGAAWSPDIFQRRSPRRQEEEIRLYRDMN
                     MNIVRSEGKMEDDNFYDLCDKYGMLVMTGWMCCGAWQYPELWDAAKRASAMASDSSVM
                     LWLRNKPSLMVWLNGSDMPPRDTSVERDYLKIEAALKWPNPTISTADASKSKVSGYSG
                     VKMDGPYEWVPPIYWETDSTRRHGGAWSYATEISPGPSIP"
BASE COUNT          608 a          683 c          800 g          566 t
ORIGIN      
        1 ggcatcatcg gcggggccat cgggccgctg atcatcggag gcattggcga cgcgttggga
       61 ctgcgcacgg gcatgttctt tctttatgtc acgctcggat atatcctcag cattggtttc
      121 tgggccagac cgctgatcac caaccagacg gttttcaata aacgcgaaaa ggcagcatat
      181 gagacagtcg aaagtgatcg ggattgatct gggcgcaacc aatatccgcg gagcggtggt
      241 gagcggggac gacctgtcgg atatcgcttc ggtgcgcatt cacagcaacg gcgaagtgga
      301 tgaagttttg ggcgatattt acagtatcat cgacacgctg atgggcccgg gggttgcggg
      361 catcggcatc ggtgtcccga gcgttgtgga tgtcgacgaa gggatcgttt atgacgtgca
      421 gtacattcca tcctgggtgg aggtgccgtt gaagcgcctc ctcgaggcac ggtatcacaa
      481 acccgtctat gtgaacaacg atgccaactg ttttgccctc ggcgagtatt atttcggtaa
      541 gggcaagggt gtgtcgtcga tggttgggct gaccatcggt accggcctgg gtggcgggat
      601 tatcattcac aacaagttat ttgccgggtc gaactgcggg gcgggcgaaa tcggctgttt
      661 gccgtacaag gataaaatcc tggaatatta ttgcagcgga tctttcttta agaatgttta
      721 tgatctcgat ggcgtcgctg tttatgaagc cgcgcgaaaa ggcgaggcca gggcgttgca
      781 gctctacgcg gaattggggc agcacgtcgg ccaggcaatc aaaatggtga tgtacaccta
      841 cgatccgcaa ctgatcgttc tcggcggctc tgtcagccag gcatatgacc tgttcaaaga
      901 gacgatgtgg aaggaggtca gaaccctagt ctatgcaaaa tctctcgagc gcattcgagt
      961 tgaagtttct aacctgaaaa atagcggcat cctgggtgct gccgcgcttt actatgatgc
     1021 aaaacaataa tttctaccgg gcgggtctcg cagcagcctt cgccctcctg gtggtccggg
     1081 ctttcccgca gactgccggg cactttagca tgaccctcaa agatgactgg caaatgcaat
     1141 cgtcaaccag ggtcccggat cagggagcgg ccatttcgga agccggctat atgccgaagg
     1201 gctggtataa ggtcagcgtc cccacgacga tcattgcggg actcttagcc aacaaagtgt
     1261 atgatttcga tccatttttt ggcatgaatc tcgaaaaact gaaggaccca acactcgatc
     1321 tcccctggtg gttccggaaa gagttcacgt tgccggctac gcaaaagggc aaggacgtgg
     1381 tcctcagcct ccatgggatc aattacaagg ctaatgtctg gctcaacggg gtgctgatcg
     1441 cggattccaa ccagatcatg aacccctacc gcatcatcga gctgaacgtc acgccatata
     1501 tccgcgagac cggtgcgaat accctcgcgc tggagatcag gcgtccgttc aatccgcaaa
     1561 aaaggggtgg cgatctggcc atcgactatg ccgactggat ccactatccg ccggactaca
     1621 atggcggcat tgtgaacgat atcgtcatca agacctatga ccgcgtgggg atcgaatacc
     1681 ccctggtgac gacccacttt gatttgccat ccctggatgt agcgcatttg acggtcgacg
     1741 cgctggttaa gaattactcc gccgggcccc aggatgtact ggtgaaagga aggatcgata
     1801 cttcacagga tggcggtggg gccgggatca ctttttcgaa aaaggtgcac ctggaagcca
     1861 acgatgtgga tgacgtggtc ttcactccag ctgagttccc tgagttgaac gtcaggcacc
     1921 cgcgcatctg gtggccctgg caatatggca agccggagct caaccggctg gaactctccg
     1981 ttgtccgcga tgacagggta agcaatacga tcgctgagaa tttcgggatc cgggagatta
     2041 cgtcggagat gatcgacaca agctcaaggg tattcgtagt gaacgggaaa cgcatcctta
     2101 tccggggcgc agcctggtcg ccggatatct tccagcggcg gtcgccgcgg cgacaggaag
     2161 aggagatccg gctttaccgc gatatgaata tgaatatcgt ccggtcggag ggcaaaatgg
     2221 aagacgataa tttctatgac ctctgcgata aatacgggat gctggtgatg accggctgga
     2281 tgtgctgtgg cgcctggcag tatcctgaac tgtgggacgc ggccaaacgg gcttcagcca
     2341 tggcctcgga cagcagcgtg atgttgtggc tgcggaataa acccagcctt atggtctggc
     2401 tgaacggaag cgatatgccg ccgcgcgata cgagcgttga gcgcgactat ctgaagatag
     2461 aagccgccct gaagtggcca aacccaacca tctcaacggc ggacgccagc aagtcgaaag
     2521 tttcaggata cagcggcgtg aagatggacg ggccctatga gtgggtgccg ccgatctact
     2581 gggaaacgga ctcaacgcgg cggcatggcg gcgcctggag ctacgccacg gaaatctcgc
     2641 caggcccgtc gatcccc
//
LOCUS       DATGKE010000079         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1530525, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000079 DATGKE010000000
VERSION     DATGKE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530525"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VKU83_01350"
     CDS             <1..643
                     /locus_tag="VKU83_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HLI92218.1"
                     /translation="GTTFNRGGTGFLIDPKGYLITNAHVVKNATIVDVQNTLGEYRAR
                     IIQLDRQADLALLKIDDTAYHAFNGLPYGISKSGPELGEDLFTLGYPRPEIVYNKGYL
                     SAKTGFQGDTTAFQLTIAANPGNSGTPVLNNDGEVVGMVTSSQKNAEGMVFAVRSREI
                     FQTIETMKEDSALQKADSTLSHLRIPLSSTIKGLDRRQQIKRIEDYIFIVKSN"
     gene            complement(670..1569)
                     /locus_tag="VKU83_01355"
     CDS             complement(670..1569)
                     /locus_tag="VKU83_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLI92219.1"
                     /translation="MQPTLLILAAGMASRYGSMKQIQGFGPSGETIMDYSIFDAIRAG
                     FKKVVFIIREDFAADFKGIFEPKLKGRVATDYVFQDLQSFAGNFAIPPDRTKPWGTAH
                     AVLCAKDAINEPFAVINADDFYGRDAFEKAHAFLTGGVNPQLYCIIGYELAKTLSENG
                     TVSRGVCQVDAAGNLLSIAERTKIYRDGSKITYEEGDRKFEVPFDSKVSMNFWGFDPA
                     VFTYTEKIFREFLAEQGNNPKAEFFIPIIGDRLIKDGKAKIKVIPTSAQWFGVTYKED
                     APAVQASLNALIASGEYPRSLWK"
     gene            1615..2520
                     /locus_tag="VKU83_01360"
     CDS             1615..2520
                     /locus_tag="VKU83_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLI92220.1"
                     /translation="MTSPPPRPSLLHPISPPAGVPGHGKADVLRLDLLHPVISGNKLF
                     KLKYHLQRAAANAGPVITFGGAWSNHLVAAAYAIRQAGLSSIGIVRGEEARSLSATLR
                     SAREYGMRLEFISRRQYAERNTPEFTNWMLARFPGAYIIPEGGSGLAGIRGCEDILRI
                     TDCSHYTHVLCAIGTGTMFLGIARASLPGQVVIGVPVLKGVDPLSLVDSNILPSDRRE
                     HAQLLTGYHFGGYARHPKPLLDFMNDFYRQTGIPSDIVYTGKLFYAYQDSLKKNIFPA
                     AARILLIHSGGLQGNLSLPPGVLLF"
BASE COUNT          581 a          721 c          634 g          612 t
ORIGIN      
        1 aggaaccact tttaaccgcg gcggcacggg tttcctcatc gatccgaagg gatacctgat
       61 taccaacgcg cacgtcgtaa agaatgcgac aatcgtagat gtccagaata cgcttggcga
      121 atatcgtgca aggatcattc aactcgaccg tcaggccgac cttgccctcc tgaaaattga
      181 tgacaccgcg tatcatgcgt tcaatggcct gccgtatggc atcagcaaat cgggtccaga
      241 attgggcgag gaccttttta ccctcgggta tccccgcccg gagattgttt acaataaagg
      301 ttacctcagc gcaaaaaccg ggttccaggg cgatacgaca gcgtttcagt tgaccattgc
      361 agccaaccct gggaatagcg gtacgcccgt attgaacaat gacggcgaag tggtgggaat
      421 ggtcacttcc agtcagaaaa atgcggaagg aatggtcttt gcggtccgct ccagggagat
      481 atttcagacc atcgaaacca tgaaggagga tagcgcgctt caaaaagcgg acagcacgct
      541 tagccatctc aggatacccc tctcctccac gattaaaggc ttggaccgcc gccagcagat
      601 caagcgcatc gaagattaca tctttatcgt taagtcgaat taaagcgcat cggccgcggg
      661 ccgcttcgct tatttccaaa ggcttcgcgg gtattccccg ctcgcgatca aggcattcag
      721 gctggcctga acggctggcg catcttcctt gtacgttact ccaaaccatt gcgccgaggt
      781 gggaattacc ttgatttttg cttttccgtc cttgatcagc cggtctccga ttatcggaat
      841 gaagaattct gcttttgggt tgttaccctg ctcagccagg aattccctga atattttttc
      901 tgtataggtg aagacggccg gatcgaaacc ccagaaattc atggatactt tcgaatcaaa
      961 cggcacttca aatttcctgt ccccttcttc gtaagtgatc ttgctgccgt cccggtagat
     1021 cttggtccgt tccgcaatcg aaagcaggtt acccgccgca tctacctggc aaacccccct
     1081 gctcaccgtt ccattctccg acagggtctt ggccagttcg tatccgataa tgcaatataa
     1141 ttgcgggttt actcctcctg tcaggaacgc atgcgccttc tcgaacgcgt cccggccgta
     1201 gaagtcgtct gcattgatca ccgcaaaagg ctcgttgata gcgtctttcg cacaaagcac
     1261 cgcatgcgcg gttccccagg gcttggtacg gtcgggaggt atcgcgaagt taccggcgaa
     1321 cgactgcaag tcctggaaca cgtaatcggt tgccactctg cctttcagct ttggttcgaa
     1381 gatgcctttg aaatcggctg cgaaatcctc ccggataata aaaaccactt ttttgaaccc
     1441 ggcacgaata gcatcaaaga tcgagtaatc catgatcgtc tcaccgcttg ggccgaagcc
     1501 ctgtatctgc ttcatgctgc catacctgga agccataccg gcagccagga tcaacagagt
     1561 tggttgcatt agcttaattt tatttttgaa atcaagacac gaaaataaaa aaatttgacg
     1621 agcccgccac ctcgaccatc attattacat ccgatcagcc cgccggcggg cgtacctggc
     1681 catggcaaag ccgatgttct ccggctggac cttcttcatc ccgtgatctc cggcaataag
     1741 ttgttcaaat taaaatatca cctgcaacgg gccgcagcga atgcagggcc tgtcattaca
     1801 ttcggaggcg cctggtcaaa tcacctcgtc gcggccgctt atgccatacg acaggcgggc
     1861 ctttcttcca tcggtatcgt tcgcggcgaa gaagctcgca gtctctcggc caccctgaga
     1921 tcggcccgtg aatatgggat gcgacttgag ttcatcagcc ggagacaata cgctgagcgg
     1981 aacacccccg aatttaccaa ctggatgctt gcgcggttcc cgggcgcata tattatcccg
     2041 gaagggggct caggtcttgc gggaattcgc ggctgcgaag atatcctgcg aattaccgat
     2101 tgcagccact acacccatgt cctttgcgcc atcggtaccg gcaccatgtt ccttgggatt
     2161 gcccgggctt ccctgccggg gcaggtcgtt atcggcgttc cggtcttgaa aggtgtcgac
     2221 ccgttgtccc tggtcgacag caacattctc ccttcggacc ggcgggagca tgcgcagtta
     2281 ctgacaggct atcattttgg cggctacgcc cgccatccga aaccattatt ggatttcatg
     2341 aatgattttt accggcaaac cggcattcca tctgatatcg tttatacggg caaactcttt
     2401 tatgcatacc aggacagcct gaaaaaaaat attttccccg cagccgcgcg gatcctcctc
     2461 atccatagcg gtgggttgca gggcaacctc tcgctcccgc ccggagtgct gttattttag
     2521 gccttggacc cggaggatcg ggcattaa
//
LOCUS       DATGKE010000080         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1530042, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000080 DATGKE010000000
VERSION     DATGKE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530042"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VKU83_01365"
     CDS             complement(<1..879)
                     /locus_tag="VKU83_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HLI92221.1"
                     /translation="MKTKIYYPFLLALISTGLHAAPRTPITATINGGNWSSTSSWNLN
                     RIPKNNDSVIIPAGFTVVFDNSYSLNNIYIALTGTLNFKQNNTLALDAASIVDILNGG
                     TLTSTHPTPNELLTINGVTKYDGKTDVTISGPVAATAMTGASPAGFSMVTLPVSFVSF
                     SAARDNGAIELAWSTGNEMNNSHFEIERSTSGSDWETIGHVAAGASCLADGYSYTDEA
                     APASQTQYRIRQVDLDGNYEYSKIVLVSAAATETAQATIFAAGKTVSIIPANGSASRL
                     IVRVFTMGGQILQQQSF"
     gene            complement(1103..1360)
                     /locus_tag="VKU83_01370"
     CDS             complement(1103..1360)
                     /locus_tag="VKU83_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI92222.1"
                     /translation="MRMTVTGLPRHYDKFDLQRLFNPFGWVDYTRIIIDPLSGLSKGR
                     GIVEMSQDADAKQAMAALQGKVLGTSPLNIKEDKKGGQKPR"
     gene            1494..2240
                     /locus_tag="VKU83_01375"
     CDS             1494..2240
                     /locus_tag="VKU83_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92223.1"
                     /translation="MFTKYLLLAFIAVCLLDSCKKNNGANSSGTPDKLKMYIEDATTS
                     GLHQVDSFTVSYDGDNRITSLASPALTFTYAYASKSFTLDLYNFSQLSIHEVAYVNGS
                     SYVDSTFQFNDTNDTTTEGYTYSGGLFTRLTTYNYSSAGSSVYSIDDYSYDNNGNAVK
                     DVNSDGSGSINMIFNFTYTTYPLKVRTSPTYLPVASKYLPATQTITDGSGHPLGTITY
                     SYVFDGSGRLTKETDSADNGDVFVKTYLYQ"
     gene            complement(2334..2573)
                     /locus_tag="VKU83_01380"
     CDS             complement(2334..2573)
                     /locus_tag="VKU83_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92224.1"
                     /translation="MPRKQRSQPGETPKPEGTPTPEITPIPAPAPEETPPGETPPGNP
                     YPEEPTPPPTPGVPVIKGVLSNMDPASFVFPFSLN"
     gene            2736..3095
                     /locus_tag="VKU83_01385"
     CDS             2736..3095
                     /locus_tag="VKU83_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLI92225.1"
                     /translation="MVSKLNWDSVPSETVNPSMSRQIVSGEKLMVARIKFKDGFVVPL
                     HFHVHEQVTQVISGKMLFWFGDDKGQTLTLEPGDVVVIPSNLPHEALMIGDVEEVDTW
                     APPRQDWLDRTDAYLRK"
     gene            3199..3459
                     /locus_tag="VKU83_01390"
     CDS             3199..3459
                     /locus_tag="VKU83_01390"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HLI92226.1"
                     /translation="MNQVFKALKDPTRRQILEMLRERDMTAGEIAERFDITWPSISHH
                     LDLLRKAGLVVSEKEGQFVCYSINTSVLEDLVEWMLQFTEKR"
     gene            3474..4139
                     /locus_tag="VKU83_01395"
     CDS             3474..4139
                     /locus_tag="VKU83_01395"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HLI92227.1"
                     /translation="MPTKRFTPVDGIFFLIWAVPLIYLAAVYRRLPARVPLHFDSAGQ
                     PDHFGTPSAFLWSIVLLQAASVGVYFLIRFLPEIDPKKKIKYSRQALVRISYALVTVM
                     AALATFLIYGTIRGRLYLDMHYVFAGIALLMAYLGNLFNNLKPNYFVGIRTPWTLENE
                     TVWKKTHQMGGRLWLAGGLFLAPACLFLPGNWATGIFAAGVAVLVLVPVLYSYLAFKK
                     ITG"
     gene            complement(4165..>4447)
                     /locus_tag="VKU83_01400"
     CDS             complement(4165..>4447)
                     /locus_tag="VKU83_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HLI92228.1"
                     /translation="GSVMATFDPTTLTLTYSISWTSLTSLPVAMHFHDNGPVIVIITG
                     FPVARTGTVSGTCALTSGQATDLANGGIYAMIHTVNYNSGEIYAPLVRH"
BASE COUNT         1037 a         1165 c         1163 g         1082 t
ORIGIN      
        1 gaaagactgc tgttgcagga tttggccacc catggtaaaa acccgtacga tcaaccggct
       61 agctgatccg ttcgccggga tgatggatac cgtcttgccg gcggcgaaga tggttgcctg
      121 cgcagtttca gtcgccgcgg cgctaaccag cacgatctta ctatactcgt agttaccatc
      181 aaggtccacc tggcggatgc gatattgcgt ctgcgaagca ggtgctgctt catcggtata
      241 gctatagccg tcagccaaac aactggcgcc ggcggcgaca tggccaatcg tttcccaatc
      301 agaaccgctg gtgctacgct ctatttcaaa atggctattg ttcatttcat tgcccgtact
      361 ccaggccaac tctatcgctc cattgtcacg ggctgcggaa aaggatacaa aagaaacggg
      421 cagggttacc atagagaagc cggcaggcga agccccggtc atggcggtgg ccgcaaccgg
      481 gccggaaatc gtaacgtcgg ttttgccatc gtacttggtg acgccgttga tggtaagtaa
      541 ttcgttcggt gtcgggtgcg tcgaggtcag tgtaccaccg ttcaggatgt cgacgatcga
      601 agcagcatcc agcgccaggg tattgttttg cttaaagtta agcgtgcctg ttagggcgat
      661 atagatattg ttcagtgaat aagagttatc gaatacgaca gtaaaacctg ccgggatgat
      721 gacagagtcg ttgttcttcg gaatacggtt gagattccag ctactggtac tggaccagtt
      781 gccgccgtta atagtcgccg tgattggagt gcggggggcg gcatggagcc cggtagagat
      841 cagggctaaa aggaacggat agtagatttt cgttttcatg gttttcagga ttgttggacg
      901 caggatgaaa gacaagcggc ataccaaaca ttccaggcat gccaaaaccg gccggccccg
      961 gcggtcaagc gtctttgtat cagctacttg taacgtcaac ggggcaggct tcccgatttc
     1021 ggaagatccg gcgcgcaaag aatattttcc gttttttgga agattgaatc gaactgggac
     1081 gatcgacggc taacccatgg agctaccggg gtttttggcc gcctttctta tcttctttaa
     1141 tattaagcgg actcgtcccc aacactttcc cctgcagcgc agccattgcc tgtttggcgt
     1201 cggcgtcctg gctcatttcg acgattcctc ttcccttgct cagaccggag agcggatcaa
     1261 taatgattct ggtatagtca acccagccga aagggttaaa aaggcgttga agatcaaact
     1321 tatcgtaatg tcgcggaagt ccggttactg tcattctcat aaacatggtt attcttctct
     1381 aaagataaat attctccgcg cgtttcgggg caaatagttc aagcaccgcc aatttgccgc
     1441 ctttcgggga acattaccta ccttggtagc aaatcactgc tcaaacttag cctatgttca
     1501 ccaaatacct gcttctagcc ttcatcgccg tatgcctgct cgactcctgt aaaaagaata
     1561 acggcgccaa ttcttccggg acccctgata aactgaaaat gtacatcgaa gacgccacca
     1621 catccgggtt gcaccaggtc gacagtttta ccgtttctta tgatggcgac aaccggatca
     1681 ccagcctggc gtctcccgcc ctcaccttca cttatgccta tgccagcaaa agcttcacgc
     1741 tcgatctgta taacttcagc cagctctcca ttcatgaggt tgcctatgtt aacgggtcct
     1801 cctatgtcga cagcaccttt cagttcaatg atacaaacga tacgacaacc gaaggataca
     1861 cctattccgg cggcctgttt acccgcctca ccacctataa ctattccagt gctggatcaa
     1921 gcgtttattc gatagacgat tacagctacg ataacaacgg gaatgcagta aaggatgtga
     1981 atagcgacgg gtcgggcagt ataaatatga tctttaattt tacctacact acttatccac
     2041 ttaaggtaag gaccagccca acctatcttc ctgttgcgtc caaatacctg ccggccaccc
     2101 agaccataac agacgggtcc ggtcatcccc tcggaacaat cacttattcc tatgtgtttg
     2161 acggttccgg ccggctaacc aaagaaaccg actcggcaga taacggcgac gttttcgtga
     2221 agacatacct ctatcaatag catgccgctt aggacgttaa gcgcttcttg gcggcggcta
     2281 agccgccctg cccgcgcagg ggtcccattg accgtccggg cagtgttttc ggctcagttc
     2341 agcgagaatg ggaaaacaaa gcttgccgga tccatattcg acaaaacgcc cttgatgacc
     2401 ggcacgcccg gggttggagg cggggtaggt tcttcgggat agggatttcc cggtggcgtt
     2461 tcgccgggtg gcgtttcctc gggtgctggc gcggggatgg gtgtaatttc cggtgtcgga
     2521 gtgccctccg gctttggtgt ctcgcccggt tgcgagcgct gtttgcgagg catagttggt
     2581 atttgcagta acccgttcaa taagtatgcc ttccggcgat ggcccccata gtcttcacct
     2641 gcagctaaaa ctgtggagga attaacccac cccgaaaagg ccgaattaga aataaatgat
     2701 ccatttcttt acattcaggt taaaaatcac ccatcatggt cagtaagctg aactgggact
     2761 ccgttccaag cgaaacggtc aatccatcca tgtcgcgcca gatcgtttca ggggaaaaat
     2821 tgatggtcgc aaggatcaaa ttcaaggacg gattcgttgt tcctttgcat ttccatgtgc
     2881 acgagcaggt gacgcaggtc atttccggaa aaatgctttt ttggttcggt gatgacaagg
     2941 gtcaaacctt gacgttggag cccggggacg tggttgtcat tccctcaaac ctcccacatg
     3001 aagcgctgat gatcggcgat gtcgaggagg ttgacacttg ggcgccgccg cgccaggact
     3061 ggttggatag aactgatgcc tacctcagga aatagtgctc tcgcaacggt cccctttgcg
     3121 actttcctgg atctcaatta agccaatgct gcgatttgga taattaaaaa atcatttaga
     3181 tatttgtcta aataagtcgt gaaccaggta tttaaagccc taaaagaccc tacccggcgg
     3241 cagatcctcg agatgctccg ggaaagggat atgactgcgg gggaaatcgc ggaacgcttc
     3301 gatatcacct ggccaagcat atcgcaccac cttgacttgc tccgaaaagc cggcctggtg
     3361 gtttccgaga aggaagggca atttgtctgc tattccatca acacgagtgt cctggaagac
     3421 ctggtggaat ggatgctaca gttcacggaa aaacgctaaa gcaaaccaag gtcatgccta
     3481 ccaaacgatt cacgccggtc gacgggatct ttttcctgat ttgggcagtt ccgctgattt
     3541 acctggcggc agtttatcgc aggttgcccg cacgagtgcc gctacatttt gattcagcgg
     3601 ggcagccgga tcatttcggg acgccctcgg ctttcttgtg gtctatcgtt cttttgcagg
     3661 cagccagtgt gggggtgtat tttctcatcc gtttcctgcc cgagatcgac ccgaagaaga
     3721 agataaaata ttcacggcag gcgctcgtcc ggatcagtta cgcgctcgtc accgtcatgg
     3781 cggccctcgc cactttcctg atttacggaa ccatccgcgg ccgcctatac ctggatatgc
     3841 attacgtatt cgcaggtata gccttactga tggcctatct cggcaacctg ttcaacaact
     3901 tgaagccgaa ctattttgtg ggcatcagga caccctggac attggaaaac gagaccgttt
     3961 ggaaaaagac ccaccaaatg ggcgggcggc tctggcttgc gggcggcttg ttcctggctc
     4021 cagcctgcct gtttctaccc ggaaactggg cgacgggcat ctttgccgcc ggggtcgccg
     4081 tcctcgttct ggtaccggtc ctatactctt atctggcgtt caagaagatt accggataag
     4141 aatcgcaccg cgaattactt gcgctcaatg ccggaccagc ggagcataga tttcccccga
     4201 gttataattg acagtgtgta tcatggcata gatccctcca ttggccaggt cagttgcctg
     4261 acccgaggtc aacgcacagg tcccgctgac agttccggtc cgggccacgg ggaaaccggt
     4321 aatgataaca atgacgggcc cgttgtcatg aaaatgcatt gcgacgggca ggctcgtcag
     4381 cgacgtccac gaaatagaat aggtcagggt cagggtggtc gggtcaaatg tggccatgac
     4441 actaccg
//
LOCUS       DATGKE010000081         9447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_186278, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000081 DATGKE010000000
VERSION     DATGKE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186278"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VKU83_01405"
     CDS             <1..439
                     /locus_tag="VKU83_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92229.1"
                     /translation="KGNNYLFVVFSSSGAAGTGAAWFVSHPPVDLKSLNYVLDLDNLG
                     DLNDTTHRLDVGGYHSAAAWASVWPNVREKRYLSFSFDSSSAGDQALFSGQHIPCLGF
                     STGSGKPESHAEKINVPGELQTVKFIYGLIQAANTRGRLAPAP"
     gene            436..870
                     /locus_tag="VKU83_01410"
     CDS             436..870
                     /locus_tag="VKU83_01410"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HLI92230.1"
                     /translation="MKFKLNIDDMTDGFFEDTRLIGIVAPVKDYQLVWQLNNRLRLDF
                     RSNHELEIQLAKKQRKYFFGVYEYREPGTALGHFLYNNQFDGEYLLPEFKHLDFLWLQ
                     KGDTVTEEKMTWLMNSVRAVPAVQLVMELRQEKIKNKGHLIF"
     gene            925..1155
                     /locus_tag="VKU83_01415"
     CDS             925..1155
                     /locus_tag="VKU83_01415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HLI92231.1"
                     /translation="MWEEKNNTLYRKFQFRNFSEAFSFMTRVALEAEKMDHHPNWSNV
                     YNTVEISLSTHDAGDIVTDKDRKLAKKIDALV"
     gene            complement(1208..3001)
                     /gene="rho"
                     /locus_tag="VKU83_01420"
     CDS             complement(1208..3001)
                     /gene="rho"
                     /locus_tag="VKU83_01420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HLI92232.1"
                     /translation="MYDILQLNDMLVPELLDIAEQLKIAGAKKLEKQDLVYKILDKQA
                     ISAKDSKSEADDEKPKRKRIIKTSTANSTEEAIVESGDDKPKKPEVKKDARKKEEKPV
                     VLKKPLRKKEEEEEEEAAAPVAKAGPRIQPVHERPKQERSDRPDQGRDNRDGRPDQAR
                     HEPRREQRHEQPKAEVEDPEETAGADEALAVQEEQEGGEAFSDGRTSDTQSARAYPQR
                     RNEAFNIEFDGVIQAEGVLEMMPDGYGFLRSSDYNYLSSPDDVYVSPSQIKLFGLKTG
                     DTVLGSVRPPKEGEKYFALLKVETINNKGPEEVRDRVPFDYLTPLFPFEKLNLFTTPS
                     DYSCRIIDLFTPIGKGQRGLIVAQPKTGKTMLLKAVANAIATNHPECYLMIVLVDERP
                     EEVTDMERSVKAEVIASTFDEPAEKHVKVSTIALQKAKRLVECGHDVVILLDSITRLA
                     RAHNTVAPASGKVLSGGVEANAMQKPKQFFGAARKIENGGSLTILATALIDTGSKMDE
                     VIFEEFKGTGNMELQLERRLSNRRIYPAIDLVASSTRRDDLLLERDVLQRMNLLRLYL
                     NDMNAEEAMNELLKRMRGTKSNEEFLASMNS"
     gene            3302..4741
                     /gene="asnS"
                     /locus_tag="VKU83_01425"
     CDS             3302..4741
                     /gene="asnS"
                     /locus_tag="VKU83_01425"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HLI92233.1"
                     /translation="MFNTRTRIKELLATAPQGQEVTVMGWVRSFRNNQFIAVNDGSTN
                     NNLQIVVELGAYGDEFLKKITPGTSLRATGSLVPSLGKGQAVEVKAASVDILGECNPE
                     KYPLQLKNRPSLEYLREMAHLRFRTQTFGAVFRIRHSLAFAVHTFFNDKGFLYLHTPI
                     ITASDAEGAGETFRVSTLPFDNPPRRDDGAIDFTEDFFGRATNLTVSGQLEGELGAMA
                     LSDIYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDLEDNMNLAEEFIRYLIRYVMDH
                     NREDLDFLAARLLDEEKQKPQAERSELGLIEKLEFVLHNKFERITYSQAIDILLQSPA
                     YKKKKFQYEVKWGIDLQSEHERYLVERHFRKPVIVTGYPKDIKAFYMRQNDDGKTVAA
                     MDILVPGIGEIVGGSQREERLERLEQRMIDMHIPVKEMWWYLDTRRFGSVPHAGFGLG
                     FERMMLFITGMTNIRDVIAFPRTPKSAEF"
     gene            4778..5704
                     /locus_tag="VKU83_01430"
     CDS             4778..5704
                     /locus_tag="VKU83_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HLI92234.1"
                     /translation="MDLAQFSGTATQKAGFVSSLGKAYEDVGFVAVRNHGVPQSIIDG
                     LYRNARAFFALPPSAKKQYEIAGLAGQRGYTSFGKEHAKGSTAPDLKEFFQFGQTVED
                     GDAVRAEYPDNVIVSELPEFNPVFGAAYRAFEASGSLLLRAIALYLGLPEAYFDPYIH
                     NGNSILRAIHYPPITSEPKSAIRAEQHEDINLITLLIGASADGLEILSKQDQWVPVTS
                     LPEQIVVNVGDMLQRLTNNKLKSTTHRVVNPPREKWGTSRYSIPFFLHPRSSMSLACL
                     DSCVDSAHPKAYPDATAGEYLDERLREIGLKK"
     gene            6041..6370
                     /locus_tag="VKU83_01435"
     CDS             6041..6370
                     /locus_tag="VKU83_01435"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLI92235.1"
                     /translation="MNIENTQSQMRKGILEFCILSVIRRGEAYPSDIVEEMKGANLNI
                     LEGTLYPLLTRLKNADMLTYRWVESNSGPPRKYFSLTPKGETFYGELQATWNELSNAV
                     HALTQKI"
     gene            6418..8694
                     /locus_tag="VKU83_01440"
     CDS             6418..8694
                     /locus_tag="VKU83_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HLI92236.1"
                     /translation="MKKIININFHSRVIPIEETAYEILQQYVESLRRYFANEEGRDEI
                     ISDIENRFSELFADKLKKGAVCITDEDVNATIASMGRPEEFEGEPEPSAHAGASGAAG
                     GAGTTGAGAGTGGASGTSGGFQQGYGPYSTTEEPRRLYRASNDKVLGGVCAGLAHYLK
                     LDPVVVRLIFVFLFFTGAGFLLYIIMWAVLPTRSLTASPASRKRLYRNPDDRVIAGVA
                     SGLAAYLHIDVWIPRLVFALPILLGVVTSIFSNIWWHAFRYNGHFIFNGFGGTFFIAY
                     IILWIVLPEATTAPEKLEMRGEKIDLESIKNTVKSDLETLKGRAKEMGTEMSERMQQF
                     GREMGSSIRQGAQNISSDVGYAARKSGTGIGHAIGVLFKAFFLVIAGLVAFALIMALI
                     GLAFGAFSNQGLVDLKGYVLDGFWQNTLAWASFILCLVVPVVALLTWLIRRVTGMRSR
                     RHYLGYVFASLWVIGIICAVVLAGMFVNNFRARAHSEEDFSLQQPSSGNLIIKAHQPL
                     VRDYYDDWWFGEGWHRHGFFYSLSEDSILLNTVQIKVLKSDDASYHLRILRGSYGSNA
                     NSALEHARQISFNVTQADSVVELPGGFPITRDQKFRNQQVIVAVYIPVGKKILLDNSV
                     KRYDWLNLNFNRQHIRWDNSWRNDEDYDDEGFLRNDNYYWDAGVQYVMTASGLQRAED
                     IEHPELREKPERPQPADRPEKKERPEQPEKANPDGRYHYQPPARSNRDSAPKTTTMIS
                     TPHSGYLVLSLFGITLFS"
     gene            8872..>9447
                     /locus_tag="VKU83_01445"
     CDS             8872..>9447
                     /locus_tag="VKU83_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92237.1"
                     /translation="MKSTPFTRFHQALGAKMVDFAGYNMPVSYTGINEEHQAVRANAG
                     VFDVSHMGEFIVKGPQALDLIQRVTTNDAARLTTGKAQYSALTNEAGGIVDDLIVYCL
                     EDRKAYMLVVNAANIEKDWNWITRHNTNGAELHNISAKTGLLAIQGPNANKIMQALTD
                     VDILNLAYYTFAKGRFAGVDNVLISATGYTGA"
BASE COUNT         2091 a         2741 c         2557 g         2058 t
ORIGIN      
        1 gaaaggcaac aactaccttt tcgtcgtgtt ttcgtcgtcc ggcgccgccg gaaccggggc
       61 ggcgtggttc gtcagccatc cgcccgtgga cctgaaatcg ttgaactatg tgctggacct
      121 cgataacctg ggtgatttga atgacacgac gcaccggctg gatgtcggcg gctaccatag
      181 cgctgcggca tgggcttccg tgtggccgaa tgtccgtgaa aagcgatacc tcagcttcag
      241 ctttgatagc agcagcgcgg gtgaccaggc ccttttttcc ggccagcaca ttccctgcct
      301 cggcttttcg accgggagcg gaaagccgga aagccatgcc gaaaagatca atgttccggg
      361 agagctgcaa accgtcaaat tcatttacgg gttgatccag gctgccaata cgcgcggccg
      421 gctcgccccc gcgccatgaa attcaagctc aacatagacg acatgaccga cgggttcttc
      481 gaggacaccc gcctgatcgg catcgtggcg ccggtgaagg actaccagct cgtgtggcag
      541 ctcaataacc ggctaaggct ggatttccgc agcaaccatg aactggagat ccagctggcc
      601 aaaaagcaac gcaagtattt cttcggggta tatgaatacc gggaaccggg cactgccctc
      661 ggccattttt tgtacaataa ccagttcgac ggcgaatacc tcctgcccga gttcaagcac
      721 ctcgacttcc tctggctgca aaaaggcgac accgtgaccg aggaaaaaat gacctggctg
      781 atgaattccg tcagggccgt tcccgcagtc cagctcgtga tggagctccg ccaggaaaag
      841 ataaagaaca aagggcacct gatattttag gctttatggc attcatggcc cgcagtttgc
      901 ggcctgaccg ataaaccgtc gtctatgtgg gaagaaaaga ataacacgct ttaccggaag
      961 tttcagttcc gaaatttctc cgaagctttc agttttatga cccgcgtcgc gctcgaagcc
     1021 gaaaaaatgg atcaccaccc gaattggtcc aatgtctaca atactgtgga aatcagcctt
     1081 tcgacgcatg acgccggaga catcgtgacc gataaagacc ggaaactcgc gaaaaagatc
     1141 gacgcgctcg tctgacgccg cgcctgctgc gacggctgcg cccgctgaag gccggcgcgg
     1201 accgaattca gctgttcatg ctggccagga attcttcgtt gctcttggtg ccccgcatcc
     1261 gtttgagcaa ctcgttcatc gcttcttccg cattcatgtc attcaggtaa agccgaagca
     1321 ggttcatccg ctggaggaca tcccgctcga gcagcaggtc gtcgcggcgc gttgaggatg
     1381 ccacgaggtc gatggcggga tagatgcggc ggttggaaag ccggcgctcc agctgcaatt
     1441 ccatgttgcc ggtacctttg aattcctcga agatgacttc atccatcttg gaaccggtat
     1501 cgatcagcgc cgttgcgagt atggtaagcg acccgccgtt ctcgatcttg cgggcggcgc
     1561 cgaagaattg ttttggcttc tgcatggcat tcgcttccac gccgcccgac aatacttttc
     1621 cggatgccgg ggctaccgta ttgtgggcgc gggcaagccg tgtgatggaa tcgaggagga
     1681 tgacgacgtc atggccgcat tccaccaggc gcttcgcctt ctgcaaagcg atggtagata
     1741 ctttcacgtg tttttcggcc ggttcgtcaa aggtggaggc aataacctcc gccttgacgc
     1801 tgcgctccat atcggtcact tcctcggggc gttcatccac cagaacgatc atcaggtagc
     1861 attcaggatg gttggtagcg atcgcgtttg ccacggcctt cagcagcatc gtcttgcctg
     1921 tcttgggttg cgcgacgatg agcccgcgct gtccttttcc gatcggggtg aaaaggtcga
     1981 tgatccggca ggagtaatca ctgggcgtcg taaaaaggtt cagcttctca aacgggaaca
     2041 aaggcgtcag gtaatcgaag ggcacccggt cacgcacttc ttccggacct ttgttattga
     2101 tcgtttcgac cttcagcagc gcaaaatatt tttcgccttc tttcggcggg cggacggatc
     2161 caagcaccgt atcgcccgtt ttcagcccga acagtttgat ctgcgaggga gaaacataga
     2221 catcgtcagg tgacgacaga tagttataat cgctgctgcg caagaagcca taaccgtcgg
     2281 gcatcatttc gaggacgcct tcggcctgga tcacgccgtc gaactcgata ttgaaggctt
     2341 cattacgcct ttggggatac gccctggcgc tctgcgtgtc gctggtacgc ccgtctgaga
     2401 atgcttcgcc gccctcctgc tcttcctgga cagccagcgc ttcgtcggcg cctgccgttt
     2461 cctccggatc ttctacctcg gccttcggct gctcatggcg ctgctcacgc ctcggttcat
     2521 gcctggcctg atcaggcctg ccgtcgcggt tatccctgcc ctggtcgggg cggtcggacc
     2581 gttcctgctt cggccgctcg tgcacgggtt ggatcctcgg acccgccttg gctaccggcg
     2641 ctgcggcttc ctcctcctcc tcttcttcct tctttcgcaa tggtttcttc agcactaccg
     2701 gcttctcttc cttcttgcgg gcgtcctttt tcacttcagg ttttttcggc ttgtcgtcgc
     2761 cgctttccac gatcgcttct tcggtgctgt tggccgtgga tgttttaatg atgcgcttgc
     2821 gcttgggttt ttcgtcgtct gcctcggatt tgctgtcctt cgcggatatg gcctgcttat
     2881 caaggatttt gtaaacaagg tcctgtttct ccagcttctt ggcacctgca atctttagct
     2941 gctcagcaat atcgaggagt tccggaacga gcatgtcgtt caattggagg atatcgtaca
     3001 taaaataact tgagagttga acaccattct caaacagtga gcctgttgta attgaaaatg
     3061 tttgaaatga tttttgataa ttgaaaaccg aatgacttaa aactgatgag ctgtgagcat
     3121 tggaaaggga aactaatcag cttaattcct ctgcaatttt agtataaata tcggaaaatt
     3181 caaaaaaaaa tttacaaacc gcctggctct cagcgctttc ccggtttcga tccgtttcgc
     3241 ccgccgccgc ttccacgctt tattgaccta tatttgcagc caatatttta gcaagggatc
     3301 catgtttaac acaagaacaa gaattaaaga actgctcgcg acggctccgc agggacagga
     3361 agtgaccgtc atggggtggg ttcgcagttt tcgcaacaac caattcatcg cggtcaacga
     3421 cggctccacg aataacaacc tccagatcgt ggtggaactg ggcgcgtacg gggatgagtt
     3481 cctgaagaaa ataacgccgg gcacctcgct gcgggcgacg ggcagcctcg taccttccct
     3541 tggaaagggg caggccgtcg aggtcaaagc cgcatccgtc gacatcctcg gggaatgcaa
     3601 cccggaaaaa tatcccttgc agctaaagaa caggcccagc ctggaatacc tgcgggagat
     3661 ggcacacctc cggttccgca cgcagacctt tggcgccgtt tttcgcatcc gccattcgct
     3721 ggctttcgcg gtccatacgt ttttcaacga caagggtttc ctataccttc atacgcccat
     3781 catcaccgca tccgacgcgg aaggcgccgg tgagacgttc cgggtcagca ccctgccctt
     3841 cgacaatccg ccacgccgcg acgatggcgc catcgatttc accgaagact tctttggccg
     3901 cgccaccaat cttacggtca gcgggcagct ggagggcgaa ctgggcgcga tggccctcag
     3961 cgatatctat acgttcgggc cgaccttccg cgcggaaaac tccaacacgg cgcggcatct
     4021 cgccgaattc tggatgatcg agccggagat ggctttttat gacctcgagg acaacatgaa
     4081 ccttgccgag gaattcatcc gctacctgat ccggtacgtt atggaccaca accgggaaga
     4141 cctcgatttt ctggcggcca ggctgctgga cgaagagaaa caaaagccgc aggcggagcg
     4201 cagcgagctc gggctgatcg aaaaactgga atttgtcctc cataataagt tcgaacggat
     4261 tacttacagc caggccatcg atatcctcct gcagtcgccg gcctataaga agaagaaatt
     4321 ccagtatgag gtcaaatggg gaatcgatct ccagtccgag catgagcggt acctcgtcga
     4381 acgccatttc aggaagcccg tcattgtgac cggttatccg aaggacatca aggccttcta
     4441 tatgcgccag aacgacgatg gaaagaccgt cgcggccatg gacatcctcg ttcccgggat
     4501 cggggagatt gtcggcggct cccagcgcga ggaaagactc gagcggctgg aacagcgcat
     4561 gatcgacatg catattccgg tcaaggaaat gtggtggtat ctcgatacgc gccggtttgg
     4621 atccgtaccg cacgccggtt ttgggctggg attcgagcgc atgatgctct ttatcaccgg
     4681 catgaccaat atccgcgatg tcattgcttt tccgcggact cccaagagcg ctgaatttta
     4741 gtattttcgc cgaaattccc tttatggcca ttcccgtgtg gacctcgccc aattttcagg
     4801 caccgccacc cagaaagcag gattcgtatc cagtctgggc aaggcatatg aagacgtcgg
     4861 atttgtcgcg gtcaggaatc acggcgtccc tcaaagcatt atcgacggct tgtaccggaa
     4921 tgccagggct ttcttcgcgc tcccgccgtc cgcgaagaaa cagtatgaga tcgccggcct
     4981 tgccggtcag cgcggctaca cttctttcgg taaggaacat gcgaagggca gcaccgcccc
     5041 cgacctgaag gagttctttc agttcggcca aaccgtggaa gacggcgacg cggtaagggc
     5101 tgaatacccg gataacgtta tcgtcagcga gttgccggag ttcaacccgg tcttcggggc
     5161 agcctatcgc gccttcgaag cttcgggcag ccttctgctg cgggcgattg ccctgtacct
     5221 cggacttccg gaagcgtact tcgatccgta tatccacaac ggcaactcca tcctccgcgc
     5281 catccactac ccgcccatta caagcgagcc caaatccgcg atccgggccg aacagcacga
     5341 agacatcaac ctgatcaccc tgctgatcgg cgcttctgcc gacggactcg agatcctttc
     5401 aaagcaggat caatgggtac cggtcacctc cctgcccgaa cagatcgtcg tcaatgtggg
     5461 cgatatgctg caacggctga ccaacaataa gttgaagtct acgacgcacc gggtggtcaa
     5521 cccgccccgg gaaaaatggg gaacatcccg gtactccatt cctttttttc tacacccgag
     5581 gagcagtatg agcctggcat gtcttgacag ttgcgttgac agcgcacatc cgaaggcgta
     5641 cccggacgcc acggccgggg aatacctcga tgaaaggttg cgggagatcg gcctgaaaaa
     5701 atagccaaaa gcgggctgcc agccgctcag atatccagga gaaaaaggag cgccaatgca
     5761 gcagcggctc cgaccagcaa ggagaggatg agtttcatat acctgggttg gggggtaggt
     5821 caaaaatacg gctttcccgc gcaataatcc agaatttttt aatctaattt taatctgagc
     5881 cgctgaaaac tccgaattgg cagccgtacc gccggccatc cgccattatt gccgtttatc
     5941 tgtcaattac tagcttataa aaattaccag ccttaaaaca gtactttgtc ttgcatagta
     6001 ctaacaaata tcctaccttt atgctgtaaa acgaaagcgt atgaatatcg agaacaccca
     6061 aagccagatg cgaaaaggaa tcctggaatt ttgcatcctc tcggtgatca ggcgcggcga
     6121 agcctatccc tctgatattg tagaagagat gaagggcgcc aacctgaata tcctggaagg
     6181 tactctctac ccgctcctta cccgccttaa gaacgctgac atgcttacgt accggtgggt
     6241 ggaaagcaac tcgggtcctc cccgcaaata tttttccctc acgccgaagg gggaaacctt
     6301 ctacggagaa ttgcaggcga cctggaacga actatccaat gccgtccatg ccctaaccca
     6361 aaaaatctga ttcctggaaa acggcacttc taaatttcaa ccgtcaaaca attttgtatg
     6421 aaaaagatca tcaacattaa cttccatagc cgggtgatcc ccattgaaga gactgcttac
     6481 gaaatcctgc agcagtacgt ggaaagtctg cgccggtatt tcgcaaacga ggaaggacgc
     6541 gatgagatca tcagcgacat cgaaaatcgc ttttccgagc tcttcgccga caagctgaag
     6601 aaaggcgcgg tctgcattac ggacgaggac gtcaatgcaa ccatcgccag catgggacgc
     6661 cccgaagaat tcgaaggcga acccgaacct tccgcccacg cgggcgcgtc tggcgcagcg
     6721 ggcggggcgg gtaccacggg tgccggtgcc ggcaccggcg gcgcctccgg gacctccggc
     6781 ggctttcagc agggatatgg cccctattcg acgacggaag aaccgcggcg gctctatcgc
     6841 gcctccaatg ataaggtcct cggcggggta tgcgccggtc ttgcgcacta cctgaagctg
     6901 gacccggtcg tggtgcggct gatctttgta ttcctctttt tcaccggcgc gggattcctc
     6961 ctatatatca ttatgtgggc agtccttccc acgcggagcc tgacggcgag ccccgcttcc
     7021 cggaaaagac tgtaccggaa tccggatgac cgggtcattg ccggcgtcgc cagcggcctg
     7081 gccgcgtacc tgcatattga cgtctggatc ccgcggctgg tctttgccct tccgatcctg
     7141 ctcggcgtgg tcacgtcgat cttctcgaac atctggtggc acgccttccg ctataacggc
     7201 catttcatct tcaacgggtt cggcggcacc tttttcatag cctacatcat cctctggatc
     7261 gtcctgcccg aagctacgac ggcgcctgaa aaactggaaa tgcggggaga aaagatcgac
     7321 ctcgaatcga tcaagaacac cgtcaaaagc gatctggaaa cgttgaaggg ccgcgcgaaa
     7381 gaaatgggca ccgagatgtc cgaacgcatg cagcagttcg gtcgcgaaat gggcagctcg
     7441 atccggcaag gcgcgcagaa tatcagctcg gacgtgggct acgcggcgcg caaaagcggg
     7501 acgggcatcg ggcacgcgat cggcgtcctc ttcaaagcat ttttcctggt cattgccggc
     7561 ctggtcgctt ttgccctgat catggccctt atcggcctgg cattcggagc gttcagcaac
     7621 cagggtctgg tcgacctgaa gggctatgtc ctcgatggct tctggcaaaa caccctggcc
     7681 tgggccagct tcatcctctg cctcgttgtg ccggtcgtcg ccctgctgac ctggctgatc
     7741 cggcgggtta ccgggatgcg ctcgcggcgg cattaccttg ggtacgtgtt cgccagcctc
     7801 tgggtgatcg gcatcatctg cgcggttgtc ctggccggaa tgtttgtgaa caatttccgg
     7861 gcgcgcgccc actcggagga agatttctcg ctgcagcagc cttcgtccgg caacctgatc
     7921 atcaaggctc atcagcccct cgtcagggat tactatgacg actggtggtt cggcgagggc
     7981 tggcaccgcc atggcttttt ctatagtctg agcgaagact cgatcctgct caatacggtc
     8041 cagatcaagg tcctgaaaag cgacgacgcc tcctaccacc tccgcatcct gcgcggcagc
     8101 tacggcagca acgcgaattc cgccctggag catgcccgcc agatctcttt caatgtcacg
     8161 caagccgata gtgtcgtcga gctgcccggc gggttcccca tcacccggga tcaaaaattc
     8221 cgtaaccagc aggtcattgt cgccgtctat atacctgtgg ggaagaagat cctgctggat
     8281 aactcggtga aacgatacga ttggctcaac ctgaatttca accggcagca catccgctgg
     8341 gataacagct ggcgcaacga cgaagactat gacgacgagg gttttctccg gaacgacaac
     8401 tattactggg acgccggcgt gcagtatgtc atgacggcca gcggcctcca gcgcgctgaa
     8461 gatatcgagc acccggaact cagggagaaa ccggaacggc ctcagccggc tgatcggcca
     8521 gagaaaaaag aacggcccga gcagccggaa aaagcaaacc ccgatggccg ttatcattat
     8581 cagccgcccg cccggagcaa ccgcgattcc gccccgaaaa ccaccaccat gatcagcacc
     8641 ccgcattcag gctatctcgt actctcctta tttggcatca cacttttttc ataaaccact
     8701 ctcgttggtt gaagttggaa cacagtcccc cgtcatctgc cggggggctc tatttttttt
     8761 ttcaaccgcc caggggcgcc aaatctgccg cgccggtgta ggggatcacg gaaggcagaa
     8821 aacttcgggg ttccggcgca aaaccataat tttgcggctc aaaaacattc catgaagagc
     8881 acgcctttta cccgcttcca ccaggccctg ggcgctaaga tggtcgactt cgccggctat
     8941 aacatgccgg tcagttatac cgggatcaac gaggagcacc aggcggtgcg cgccaatgcc
     9001 ggcgtcttcg atgtaagcca tatgggcgag tttatcgtca aaggaccgca ggcgctcgat
     9061 ctgatccagc gggtcacgac caatgacgcg gcgcggctga cgacgggtaa agctcagtac
     9121 agcgccctta ccaacgaggc gggcgggatc gtcgatgacc tgatcgtcta ttgcctcgag
     9181 gacagaaagg cctatatgct cgtggtcaac gccgccaata tcgaaaagga ctggaactgg
     9241 atcacgcgcc acaatacgaa tggcgccgag ttgcacaaca tctcggcaaa aacgggactg
     9301 ctggccatcc agggtccgaa cgcgaacaag atcatgcagg cactcaccga cgtcgacatt
     9361 ctcaacctcg cctattatac cttcgcgaag ggccggttcg ccggcgtgga caatgtcctc
     9421 atcagcgcca ccggctatac gggggcg
//
LOCUS       DATGKE010000082         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1325578, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000082 DATGKE010000000
VERSION     DATGKE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325578"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VKU83_01450"
     CDS             complement(<1..546)
                     /locus_tag="VKU83_01450"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92238.1"
                     /translation="MLKVLKYMVNKRNLPVVLLILGAGVFVAFRTFGIGGNPPSKDET
                     ILHNVGEYLQELHYSPKPIDDNFSLEVFNKYLGEVDGEKNIFFQSDIDGLKAKYGKEI
                     DDEILGKPIAFVPAVNALYKKRLIETEQIYKSILAKPFDFSKDEDYNQNFDKLSFPHS
                     DADRREAWRKQLKYLALVRYAD"
     gene            complement(667..740)
                     /locus_tag="VKU83_01455"
     tRNA            complement(667..740)
                     /locus_tag="VKU83_01455"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:His,seq:gtg)
     gene            883..1497
                     /locus_tag="VKU83_01460"
     CDS             883..1497
                     /locus_tag="VKU83_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92239.1"
                     /translation="MTRISWWLYTIPFISAFIHWLTIRMALKLLFHPRQPRRIFGLRL
                     QGVFPKKQRQIAESLGRIVGQQLLSFGEIGKTITDPANVNRILPVVEEHIDDFLRNKL
                     KESMPMIAMFIGEKTIAQLKAVFMQELQELFPVILQRYLGHLRDDLDLERMVVEKITG
                     LSNDRLEGMLIEFLTREFRFVEGIGAALGFLIGMLQILITLSVK"
     gene            1613..>4088
                     /locus_tag="VKU83_01465"
     CDS             1613..>4088
                     /locus_tag="VKU83_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92240.1"
                     /translation="MRKSWLLLLATALTLNGFAQYPGGGGGGRRGGAVPSIGHFYGKV
                     VDAKTNKGLDGASVELIQSKYSPAAHGPKDTVISGMITDRHGDFSLENLPIFGNFRLR
                     ITAIGFKTVEQKVAFDLRAMRGGNGNNMAPPVDQENQDQQASRAMQALDAVDKDLGNI
                     KLAEEAETMASAVVTASKPLISMGVDRKVYNVEKDISAQGGTGVDVMRNIPSVQVDID
                     GNVTLRNSTPTIFVDGRPTTLTLDQIPADAIESVEIITNPGAKYDASGGTAGILNIVL
                     KKNRKAGYNGNVRAGIDQRGKYNLGGNFNMKEGKINAFLAGSYNQRKSISPGTTSRYT
                     FNQPGRFPDDSLIENDNNVNLGYFAFGRGGIDYLMDNRNTLSLSGNIVHGTFQPHTNS
                     NIDVDTLYPSGTTYSYTNRLSTSNSVFNNHGGMLSYKHNFPKSGEELDADVNYSQGTN
                     NNTNLIASNIYDIKGGPLTTIYNQQQTINGTNKYFTAQTDYSLPFNEKSKFEAGARVQ
                     VRNIYSLTDLDTVGANNKFVVEQLLSSAFQYQDRITAAYVTYGNMYKNFTYQLGLRGE
                     SYQYNGQENFTDVDQFGRQFDTVGTFKSNFPLSLFPSVFLTQKLNDNQNLSASYTRRI
                     DRPQFQQLFPFTDYSDSLNLSRGNPNLRPQFTNSMEIAYQLNYRATNTFLASVYFKNT
                     TDLITRAADTGINPVTHEPVIINSYINAKSGFVGGLELIGRNAITKWWDLTTNVNVYT
                     SKINTVDTGIFAVPPIGEIYSVFAKAISSFKLYKAFTLQVSGDYTSKTQLPPGGSSGG
                     GGGHGWGPTVSGNSQGYSKPSGGLDAS"
BASE COUNT         1034 a         1126 c         1036 g          892 t
ORIGIN      
        1 atcggcatac cggaccaggg ccaggtactt cagttgttta cgccatgctt cacggcgatc
       61 ggcgtcgcta tgaggaaagg aaagcttgtc gaagttctga ttataatcct catcctttga
      121 gaagtcgaag ggcttagcca gaatggactt gtagatctgt tccgtttcga tgagccgctt
      181 cttgtacagc gcattcaccg ccggaacaaa agcgatcggc ttcccgagga tctcgtcatc
      241 gatctccttt ccatattttg ccttcaggcc gtcgatgtcg gactgaaaga agatgttctt
      301 ttccccatcc acctcgccga ggtatttatt gaagacctca agcgaaaaat tgtcatcgat
      361 gggcttcgga ctgtaatgca gttcctgaag gtactcaccg acattatgaa ggatcgtctc
      421 atccttcgaa ggcggattgc caccgatccc aaacgtcctg aaagcgacaa aaactcctgc
      481 cccaagaatc agcaggacga ccggcagatt tctcttattg accatatact tcaatacttt
      541 aagcataact atcaaaaata acgattattg caatgggatg ttgtcgccct caatcaatat
      601 taacagccgc ttataaaaaa agctccgacc aggcgcttag caccttgtga tcgaagcttt
      661 ttcgcgggtg gctgatgggg ctcgaaccca cgaccctcag aaccacaatc tgatgctcta
      721 accaactgag ctacagccac cgggtggaga tttcaaagcg cgaaattagc ataaaatgat
      781 tctttttcaa aaacattttg ccgttaattt gcgggcccaa aaacagcgcg gtagcgcggc
      841 gggaacgaac cgaaacacat ccggcgcatc aacagaagaa ccatgacaag aatctcctgg
      901 tggttgtata caatcccatt catctccgct tttatccatt ggctgacgat ccggatggcc
      961 ctgaaactcc tgtttcatcc acggcaaccg cgacggatat tcgggctcag gctccagggc
     1021 gtgtttccga aaaagcagcg gcagatcgcg gagagcctgg gccgcatcgt cgggcagcag
     1081 ttgctctcct tcggggaaat cgggaagacc atcaccgatc ctgcgaacgt caaccggatc
     1141 cttccggtcg tcgaggaaca tatcgacgat ttcctccgaa acaagctgaa ggagtcgatg
     1201 cccatgattg cgatgttcat cggcgaaaaa acgatcgccc agctgaaggc tgttttcatg
     1261 caggaattgc aagaactttt ccccgtcatt ttgcagcgtt acctgggcca tttgcgggat
     1321 gatctcgacc tggaacgaat ggtagtggaa aagatcaccg gactttcgaa tgatcgcctc
     1381 gaggggatgc tgattgaatt cctcaccagg gaattccggt tcgtcgaagg gatcggtgca
     1441 gccctggggt ttctcatcgg aatgctgcag attctcatca ctttatcggt gaaataaggt
     1501 gcagaatgcg cattacccct ttcgcatttg ttttataccg cttgtataga ttgatctgga
     1561 ttactggtcc aaatgatcgg aaatttgcgg agaaaatcga atctttttac taatgagaaa
     1621 atcctggctt ttactgttgg ctacggcttt aaccctgaat ggctttgcgc aatatccggg
     1681 cggcggcggc ggcgggcgcc gcggcggcgc cgtcccctcg atcggtcatt tttatggcaa
     1741 ggtcgtggat gcaaaaacga acaaggggct cgacggtgct tccgtcgaac tgattcaaag
     1801 caaatacagt ccggctgcgc atggccccaa ggacaccgtt atttcaggca tgatcaccga
     1861 ccgtcacgga gatttcagcc tcgaaaacct tcctattttc ggcaatttcc gcctccggat
     1921 cacggccatc ggctttaaaa ccgtcgagca gaaggtcgct ttcgatctgc gcgccatgcg
     1981 cggcggcaac ggcaacaata tggcgccgcc cgtcgaccag gaaaaccagg accagcaagc
     2041 ttcccgggcg atgcaggcgt tggacgcggt cgacaaggac cttggcaaca tcaaactggc
     2101 cgaggaagcc gagaccatgg cctccgcagt cgtcacggcc tcaaagccgc ttatcagcat
     2161 gggtgtagac cggaaagtct ataatgtcga aaaggacata tccgcgcagg gcggaaccgg
     2221 cgtcgacgtc atgcggaaca tcccttctgt gcaggtggat atcgacggga acgtaacctt
     2281 gcgcaacagt actcccacga tctttgtgga cgggcggccg acgacgctca cccttgacca
     2341 gatcccggca gatgcgatcg agagtgtcga gatcatcacc aatcccggcg ccaagtatga
     2401 tgccagcggc ggtactgcag gcatcctgaa tatcgtcctg aaaaagaacc ggaaggccgg
     2461 ctataacggg aatgtgcgcg ccggcatcga ccagcgcgga aaatacaatt tgggtggaaa
     2521 cttcaacatg aaggaaggaa agatcaacgc ttttctcgcc ggtagttaca accagcggaa
     2581 gtccatatcg ccgggtacga ccagccgcta cacattcaac caacccggcc ggttcccgga
     2641 cgatagcctg atcgagaacg acaataatgt gaacctcggt tattttgcct tcggccgcgg
     2701 cggtatcgac tatctcatgg acaaccgcaa tacgttgtcg ctgtccggca atatcgtcca
     2761 tgggaccttc cagccccata ccaacagcaa tatcgacgtg gacacgctgt acccttcggg
     2821 cacgacctat tcctatacca atcgcctgtc taccagcaat tcggtcttca acaaccatgg
     2881 cggcatgttg tcctacaagc acaatttccc aaaatccggg gaggagctgg acgcagacgt
     2941 taactacagc cagggtacga acaataacac caacctgatc gcctccaaca tctatgacat
     3001 caaaggcggt ccgttgacca ctatctataa ccagcagcaa accatcaacg gaaccaataa
     3061 atattttacc gcgcagaccg actatagcct gccattcaac gagaagtcca aattcgaggc
     3121 cggggccagg gtccaggtcc gcaacatcta cagtctcacc gatctggata cggtaggcgc
     3181 gaataataag tttgtggtcg agcagttgct gtcttcggcg ttccagtatc aggaccgcat
     3241 aaccgcagca tatgttacct acgggaacat gtacaagaat tttacctacc agcttggctt
     3301 gcggggggaa agttaccagt ataacggcca ggaaaatttt accgatgtcg atcagttcgg
     3361 ccgccagttc gataccgtcg gcaccttcaa gagcaatttc ccgctgagct tgtttccaag
     3421 cgtattcctg acgcaaaaac tgaatgataa ccagaacctc tccgcgagct acacccggcg
     3481 gatcgaccgg ccgcaattcc agcagttgtt cccattcact gattattccg attccctcaa
     3541 cctgagcagg gggaatccca atctgcggcc gcagttcacg aactcgatgg aaatcgccta
     3601 tcagctgaac tatcgggcaa cgaacacctt cctggcatcg gtctatttta agaatacgac
     3661 cgacctgatc acaagggcgg ccgacaccgg cattaacccg gttacgcacg agccggtcat
     3721 tatcaattcc tatatcaatg cgaaatcggg gtttgtcggc ggtctcgagc tgatcggccg
     3781 gaacgcgatc acaaaatggt gggacctgac gaccaatgtg aatgtctaca cctccaagat
     3841 caatacagta gacaccggta ttttcgcggt gccgccgatc ggcgagatct atagcgtttt
     3901 cgcgaaggcg ataagcagtt tcaagctgta caaggccttt accttgcagg tcagcggcga
     3961 ctacacgtca aaaacgcaat tgccgcccgg cggcagctcc ggcggcggcg gcggccatgg
     4021 atggggaccg accgtcagcg gaaattcgca gggctacagc aaaccttcgg gcgggttgga
     4081 tgcctcga
//
LOCUS       DATGKE010000083         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_185228, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000083 DATGKE010000000
VERSION     DATGKE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185228"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VKU83_01470"
     CDS             complement(<1..487)
                     /locus_tag="VKU83_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HLI92241.1"
                     /translation="MRKTAGSTQATRVSIQGYEGSFHQVAAEEFFGMEVQVIPCATFR
                     EVVRVASSAGESNGGVMAIENSIAGSILPNYNLLQQSALRVVGEIYLQIGQNLLVNKG
                     VKLEDIREVHSHQMALLQCIDYLEKHPAWKLVETEDTALSARHLAQHHSRHVAAIASR
                     RA"
     gene            complement(784..1347)
                     /locus_tag="VKU83_01475"
     CDS             complement(784..1347)
                     /locus_tag="VKU83_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92242.1"
                     /translation="MHEIEPFYNWRHIYVSEEDERSPFYGRIYSEFEFSQTIYNYYIH
                     PQWDEFGSRTLYMKVLLADYDEHYAVIELFGEWNDAIENDIMNVKREVTDKFFEGGIT
                     KFILIAENVLNFHSGDKDYYDEWFEEVTDAGGWIACLNMPEQTQYDFRKAKLNRYIEL
                     VQLDNWRTYKPFHLFKLLDQHINNRLT"
     gene            complement(1340..2179)
                     /gene="murQ"
                     /locus_tag="VKU83_01480"
     CDS             complement(1340..2179)
                     /gene="murQ"
                     /locus_tag="VKU83_01480"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HLI92243.1"
                     /translation="MDIEKVTEEESRYHDLEKMSVHEILEAINREDKSVPDAVARAIP
                     QVEQLVVAITDKMLAGGRLFYIGAGTSGRLGVLDASEIPPTYGMPFDLVIGIIAGGES
                     AIRKPVENAEDDREQGWLDLGEHEVSTKDIVVGIAASGTTPYVEGALKECQKAGIPTG
                     CVVCNPGSPIAAVADYPVEVVVGPEFVTGSTRMKSGTAQKLVLNMLSTAVMIRIGRVE
                     DNKMVNMQLTNSKLLDRGTRMVMDGTGLKDYERARELLLRTGSVKKAVESGRGWQDPG
                     KYA"
     gene            complement(2188..>2372)
                     /locus_tag="VKU83_01485"
     CDS             complement(2188..>2372)
                     /locus_tag="VKU83_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92244.1"
                     /translation="QHLCKYPESWKLPIHFVGGVAYGFRDVIRELCSGYEFELGKILR
                     KPMDGLISYHRKALSK"
BASE COUNT          505 a          720 c          518 g          629 t
ORIGIN      
        1 cagcccggcg actggcaatc gcggccacat ggcggctgtg atgctgcgcc agatgccggg
       61 cgctgagcgc cgtatcttcc gtctcaacca gcttccacgc cggatgcttc tccaggtaat
      121 cgatgcactg caacagggcc atctggtgcg aatgtacctc gcggatatct tccagtttca
      181 cgcccttatt gaccagcagg ttctgcccga tctgtaaata aatttcgccg acgacccgta
      241 gcgcgctttg ctgcaggaga ttgtaattag gcaaaatact gccggcaatt gaattctcga
      301 tcgccatcac ccctccgttg ctttcgcctg cactcgacgc gacccgcacg acctcccgga
      361 atgtcgcgca cggaatgacc tggacctcca tcccgaaaaa ctcctccgca gccacctgat
      421 gaaagctgcc ttcataaccc tggatagaga cccttgtcgc ttgagttgaa cctgctgtct
      481 ttcgcatgtc tctgctaatt gcgcccatag gctgataaag caaaagtccc ggcttttggc
      541 cgggacttta ttttgttagc gttttattta tttacttgtt aacaaaagcc atcccggtct
      601 acctccgaag tagaagtaaa aataaaagta aaaaaaccgg tatgccttcg taatactcat
      661 attaaggctt caaaattagc tacaaatccg gaataacaaa atttattttg gaatttgcct
      721 ggcttgctcc cttgcggcga tgccccagcc gtccgccgcc tttcatcgca gtcatttgcc
      781 gccctaagtc agccggttat tgatatgctg atcgagcagt ttgaacaaat ggaatggctt
      841 gtacgttcgc cagttgtcca actggaccag ctctatgtac cggttcagct tggcctttcg
      901 gaaatcatac tgcgtctgtt ccggcatatt aaggcaggcg atccatcctc ctgcatcggt
      961 cacttcttca aaccactcgt catagtagtc cttgtcgccg ctgtggaagt tgagtacgtt
     1021 ctccgcgatc aggatgaact tcgtgatccc gccttcaaag aatttatccg tcacctcccg
     1081 cttgacgttc atgatatcgt tctcaattgc gtcgttccat tcgccgaaca gttcgatcac
     1141 tgcataatgc tcgtcatagt ccgccagcag tactttcatg taaagggttc gggagccgaa
     1201 ttcgtcccat tgcgggtgta tgtaatagtt atagatggtc tgtgaaaatt caaattcaga
     1261 atagatcctt ccatagaacg gcgatcgctc gtcctcttca ctcacataaa tgtggcgcca
     1321 gttgtaaaaa ggttctattt catgcatatt tacctgggtc ctgccaaccc ctccccgatt
     1381 ctaccgcctt cttgacgctt ccggtcctca gcaacagttc cctcgcccgt tcataatcct
     1441 tgagcccggt accatccata accatgcgcg taccccggtc aaggagcttg ctgttcgtca
     1501 actgcatatt taccatcttg ttatcctcta cgcgccctat ccggatcatt accgccgtcg
     1561 acagcatatt caacaccagc ttttgcgccg tcccgctctt catgcgtgta cttcccgtga
     1621 cgaactcggg accgaccact acttcaaccg ggtagtctgc aaccgctgct atgggtgacc
     1681 cgggattgca gacgacgcaa cccgtcggta tccccgcttt ttgacattcc ttcaatgcac
     1741 cctcgacata tggcgttgtc ccgcttgctg caatgccgac tacgatatct ttagtggaaa
     1801 cctcgtgttc gccgaggtcc agccagccct gttcccggtc atcctctgca ttttccaccg
     1861 gtttgcggat cgcgctctcg ccgccggcga ttattccgat gaccagatca aaaggcatac
     1921 catacgtcgg cgggatctcg ctggcatcca gcactcccag ccgcccgctt gtcccggccc
     1981 cgatgtaaaa taaccggccc cccgccaaca tcttatccgt gatcgccacc accagttgct
     2041 cgacctgcgg gatggccctt gccacagcat cgggaacgct cttgtcttcc ctgttgatgg
     2101 cttcgaggat ttcatggacg ctcatttttt caaggtcgtg ataccggctt tcctcttccg
     2161 ttaccttttc aatatccata cttgcgctta ttttgataag gcctttcgat gataactgat
     2221 gaggccatcc atcggcttcc ggagaatctt tcccagctcg aattcatagc ccgagcacaa
     2281 ttccctgata acatccctga acccgtatgc cacaccgccc acaaagtgga tcggcaattt
     2341 ccaactttcg gggtacttgc acaggtgctg ga
//
LOCUS       DATGKE010000084         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1528245, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000084 DATGKE010000000
VERSION     DATGKE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528245"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1704
                     /locus_tag="VKU83_01490"
     CDS             31..1704
                     /locus_tag="VKU83_01490"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HLI92245.1"
                     /translation="MSDPRNLSPTERIKQHSDALRGTLTEGLRDELTGAIADDDQALV
                     KFHGMYLQDDRDRREERAEKKLERLYAFMVRLRVPGGFFTPEQWIATHHIAGENATGA
                     IKITTRQTIQLHGILKSRVKPTLAAFNAVALDSIAACGDVNRNVTCSANPLQSPIHEQ
                     VHAYARQISQMLLPKTAAYYEIWLDQEKLSDKSLEADPLYQDRYLPRKFKIGLAIPPN
                     NDVDVLTNDLGLIAIIEEGQLKGFNLAIGGGLGTTHGNPETYPRLATVIGFVPADDRL
                     FKAVYEVVTIQRDYGNRSDRKLARLKYTLDKYGASWYRQELEKRTGFALEEARPFTFN
                     DRTERFGWQEDHKGVWHYTLFVENGRVLDDETLAMKTALLEVAATRKANFRFTVNQKI
                     IIADVDKNDKELIDGILEKYGLIAHTEAASAIRRNSIACVALPTCGLALAESQRYLPA
                     LIGRIEALLSVHGLQEEQIIIRMTGCPNGCGRPYAAEIGFVGTAPGRYNLYLGGDHEG
                     TRLNRLYKENLDEAAILADLDLLFRLYKKERITGERFGDFAWRQRWYKQ"
     gene            1775..3004
                     /locus_tag="VKU83_01495"
     CDS             1775..3004
                     /locus_tag="VKU83_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLI92246.1"
                     /translation="MHRTITALILGQLLWATATQAQTADEILNILTRKGTLTEREADS
                     IRHESALRQRRSEATADSFPLRLGRSLSLSGYSQVVYQNFQHPLTGKYADGFAIKRAR
                     LDFQGQFSSQFDYRLLVDFIGQSGANGSAATGGQQISPFLVEANITYKPFKWLNVKAG
                     QLIVEFSQENIIQDRNLDLIERSQVVCALVARKGDAANGIIDSVGNQNGRDIGIQASG
                     SVGRIGDRYLLDYYVQVLNGAGINTLDNNPSKDFNTRLVLHPLDILSIGGSYYDGYDR
                     FIASTAKDQRRLRWGLEADLEWSGLSARSEYIHGQEGSANLKDQNGISTAISHQGWYA
                     QLGYFFFRHKLQGVVRYDSYDPNSSKPAVTSTYYDLGLNYFFNVWTKLQFYYSVRDQQ
                     GAAINNNFFEAQLQLAF"
     gene            3069..>3530
                     /locus_tag="VKU83_01500"
     CDS             3069..>3530
                     /locus_tag="VKU83_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HLI92247.1"
                     /translation="MENEKSIPATSGNNLFPLFVKLEELKLLIVGGGNVGLEKLQTVL
                     HNSPSTTITLVAPEIVGPIRDLAATYPNIRLLNKSWDPSDLDLADLAIVAVNDRAVSE
                     AVAREARARGILVNVADTPDLCDFYLGSVVRRGNLKIAISTNGKSPTVAKRL"
BASE COUNT          832 a         1052 c          943 g          703 t
ORIGIN      
        1 cggtatgtaa aagacgttta ttgacaggat atgagtgacc ccaggaattt atccccgaca
       61 gaacgaatca agcaacacag cgatgccctc cggggcaccc tgaccgaagg cctgcgcgac
      121 gagctgaccg gcgccatcgc cgacgatgac caggcgctgg tcaaattcca tggcatgtac
      181 ctccaggatg accgcgaccg gcgggaggag agggcggaaa agaagctgga gcgcctttac
      241 gcttttatgg ttcgccttcg ggtgcccggc ggattcttca cgccggaaca atggatcgcg
      301 acccaccata tcgccggtga aaacgccacc ggcgccatca agatcaccac ccggcagacc
      361 attcagctgc acggcattct caaaagccgc gttaagccta cgcttgccgc cttcaatgcg
      421 gtggctctcg attccatcgc ggcctgcggg gacgtgaacc gcaacgtgac gtgctcggcc
      481 aacccgcttc agtcgcccat tcatgagcag gtccacgcct atgcacgcca gatcagccaa
      541 atgctgctgc caaaaaccgc ggcttattac gagatctggc tcgaccagga aaagctttcg
      601 gataagagcc tggaagcgga cccgctttac caggaccggt atctgccgag gaaatttaag
      661 atcggactcg ccattccgcc caataacgat gtcgacgtgc ttaccaatga cctcggattg
      721 atcgccatca tcgaagaagg tcagcttaag ggtttcaacc tggccatcgg cggcggtctt
      781 ggcactaccc acggcaatcc ggagacctat ccgcgcctgg ccaccgtaat tggttttgtt
      841 ccggccgatg accggctctt caaggcagtc tacgaagtcg tcaccatcca gcgcgactac
      901 ggcaaccgca gcgaccgcaa actcgcgcga ctcaaataca ctctcgacaa atacggcgcc
      961 tcctggtatc ggcaggaatt ggaaaaaagg accggcttcg cgctcgaaga ggcgcggcca
     1021 ttcaccttca acgaccggac ggaacgcttc gggtggcaag aggatcacaa aggagtgtgg
     1081 cactatacct tgttcgtcga gaacggacgc gtgctcgacg acgaaaccct ggccatgaaa
     1141 acggcgctgc ttgaagtggc tgcaaccagg aaagcaaatt tccgctttac ggtcaatcaa
     1201 aagatcatca ttgcggacgt agataaaaac gataaggagc ttatcgacgg aatactcgaa
     1261 aaatacgggc tgatcgcaca tacggaggca gcgtcggcga tcaggaggaa ctccattgcc
     1321 tgcgtggcac tccccacctg cggcctcgcg ctggctgagt cgcaacgcta tcttcctgcg
     1381 ctgatcggtc ggatcgaagc gctcctttcc gtgcacggcc tgcaagaaga acagatcatc
     1441 atccggatga ccggctgtcc caacggctgc gggcggccct atgccgccga gatcggtttc
     1501 gttggcaccg cccctggccg atataacctg tacctgggcg gcgaccatga aggcacgagg
     1561 ctcaaccgcc tttacaaaga gaacctggat gaagccgcta tcctggccga cctggacctg
     1621 cttttccggc tgtataagaa ggaacggatt accggcgaaa ggtttgggga tttcgcctgg
     1681 cgccaacggt ggtacaagca ataaccgctc cggccgctga ccgccaacgg tcggtttttt
     1741 ttactttaat actctactaa attgatagac taatatgcat cgtacaatta ccgccctcat
     1801 cctgggccaa ctgctatggg ccaccgccac gcaagcacaa accgccgacg agatcctaaa
     1861 catcctgact cgaaaaggaa cgctgaccga acgcgaagcc gacagcatcc gccacgaatc
     1921 agccctgcgc cagcgacgga gtgaagcgac tgccgactcc ttccctctcc ggctcggccg
     1981 ctcgctgagc cttagcggct attcacaggt agtttaccag aatttccaac atcccctgac
     2041 cgggaaatat gctgacggct ttgccatcaa aagggcccgg ttggattttc aggggcagtt
     2101 ttcttcgcaa ttcgactacc gcctgctcgt cgatttcata gggcagagcg gcgcgaatgg
     2161 atccgccgct accggcggcc agcagatttc tcccttcctg gtagaggcaa atattaccta
     2221 caagccgttt aaatggctga atgttaaagc gggtcagctg atcgtcgaat tctcgcagga
     2281 gaacatcatc caggaccgaa acctcgacct aatcgaacgc agccaggtag tctgtgccct
     2341 cgtggcacgt aaaggcgatg ccgcgaacgg gatcatcgac tccgtcggca accagaacgg
     2401 acgcgacatt ggcatccagg ccagcggcag cgtgggccgg atcggcgaca gatatctcct
     2461 ggattattat gttcaggtgc tgaacggcgc cggtatcaat acccttgaca acaacccatc
     2521 caaggacttc aacacccgcc tggtcctgca tccgctggac atcctcagca ttggcggctc
     2581 ttattatgac gggtatgacc ggttcatcgc cagcaccgcc aaagaccagc gccggctgcg
     2641 atggggcctc gaggctgacc tggaatggag cgggctttcc gccaggagcg aatatattca
     2701 tgggcaggaa ggcagcgcca acctgaaaga ccagaacggg atctccactg cgatcagtca
     2761 tcagggctgg tatgcccagc ttgggtattt ctttttccgg cacaagctcc agggcgttgt
     2821 ccgttacgat tcgtacgacc cgaacagctc aaagcctgcc gtcaccagca cgtattatga
     2881 tcttggcctg aattattttt tcaatgtctg gacgaaactg cagttctatt attcggtgcg
     2941 ggaccagcaa ggagccgcaa tcaataataa ttttttcgaa gcgcagctgc agctggcgtt
     3001 ctagcctgac aagtccacca ttcttatagg cgaatcaagc caaaaatgac tatttttata
     3061 cattattaat ggaaaacgaa aagagcatac ccgcgacgtc cggcaacaac ctgttcccgt
     3121 tgttcgtgaa gctggaagag ctgaaattgt tgatcgtcgg cggtggcaat gtcggactcg
     3181 agaagctgca aaccgtactg cataactccc catcaacgac tatcaccctg gtcgcgcccg
     3241 agatcgttgg gccgatccgg gacctggcgg cgacctatcc gaacatccgc ttgctgaaca
     3301 agtcctggga tcccagcgac ctggaccttg cggatctggc catcgtcgcg gtcaatgacc
     3361 gcgccgtcag cgaagcggtc gcccgtgaag cccgggcccg tggcatcctg gtcaatgttg
     3421 ccgacacgcc tgatctatgc gatttttacc tgggttctgt ggttcgcaga ggaaatctga
     3481 agatcgccat ttccaccaac ggcaagtccc cgaccgtcgc caaacgcctt
//
LOCUS       DATGKE010000085         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1012598, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000085 DATGKE010000000
VERSION     DATGKE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012598"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VKU83_01505"
     CDS             <1..1083
                     /locus_tag="VKU83_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92248.1"
                     /translation="MINSELEVGTDATFTPFEGAWVSKLVFSGSYWHRQSKDVYQNAQ
                     VAPSTGYATHLDNLSTIVSHGVDLSLDASLYKGRNITWDLSARWGYSRAVVTKVANGQ
                     SIVNFCFAVTQGQQLGLFYVQTPLHSINQLGPDGKTPLINPANQGKYTMTSTGDVVLA
                     SDNYVQYTPATDLSVVGHAYPDFTSSLINRFTFFGRLSLSFQFDWIHGNSIYNVTKQW
                     LYRPTGGTGGQGGESSDLDKKLTIAGQTGSFVNYYNSLYNVGLPVSPFVESGSYIRLR
                     DLSIGYDLSRYVTGTKAIKRLTITASGRNLLTFTKYSGLDPENTGAYDEQGDDLSHSR
                     TGAFSGVDFYGTPNLRSYQFSLNVGF"
     gene            1109..2797
                     /locus_tag="VKU83_01510"
     CDS             1109..2797
                     /locus_tag="VKU83_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI92249.1"
                     /translation="MNKQLYSPRLIRRAILPLLACTLLLGACQKKILGILNPNLPTPQ
                     GSLVTEGGIDAFAEGIFYKWIAFETGDGNLNFFDIAWYIESNMGDEDFTPYSNYGSRY
                     PMNIASITLPAPYNTVVNNPSGFATQLDILRSFNSRSAGDGNAIQYEWDCFYYVNAQA
                     NILLNALDNPALKLSGDATTKKALLQAWAYYWKGYAYSKLGSMYLAAVIDDSPDSTVK
                     SLTSNIYVAHDQVIAAANKNFNQAASLFSGITEDADYDATFRAIIPSFNAPTQVITPT
                     MWVHQINSFEARDYLVNHKVDSMTSADWNNVLTLATAGMQQGDHTLMWGVAPAGVPDL
                     TQNLGFLFHPMMLHTPSGALSFVSERLIQDIQPGDARLNYFEPYPTGTVVNVLNRGIQ
                     FGTRYAPVDIENGGRYATENFSSQALVPIAPTWEENALMIAECNIYLGNVDAGLQLID
                     QVRASQGAGLPVLAGSGMSQDSAIAQLRSERRIGLYLHNVSWYDARRWGITAPAAQGG
                     GRIGNIVVPGSLIGANSYQVLPCFINYSFSDYWDVPKNETDFNPPAAGSAPVAN"
     gene            complement(2885..>3306)
                     /locus_tag="VKU83_01515"
     CDS             complement(2885..>3306)
                     /locus_tag="VKU83_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HLI92250.1"
                     /translation="GGNLRLQNVKGTVHATTSGGDIDGDEIAGDLRTSTSGGSIHLDD
                     MRCSLNASTSGGNIDVTMKSMGKFLELENSGGSIHLSVPSGQGYNLAISGDRVHTDKL
                     SNFSGKMDEHSIEGTLNGGGIPIKVRGSSGHVTLSFQ"
BASE COUNT          738 a         1000 c          831 g          737 t
ORIGIN      
        1 atgatcaact cggaactgga ggtaggaacg gacgcaacgt tcacgccttt cgagggcgcg
       61 tgggtgtcca agctggtatt cagcggcagc tattggcacc gacaatccaa ggacgtctat
      121 cagaacgctc aggtggcgcc ttccaccggt tatgccaccc acctggacaa cctgagcacg
      181 atcgtttccc acggggtgga cctttcactt gacgcttccc tgtacaaagg ccgcaacatc
      241 acctgggacc ttagcgcccg gtgggggtat agccgcgccg ttgtgaccaa ggttgccaac
      301 ggtcaaagca ttgtcaactt ctgttttgcg gtaacccaag ggcagcaact cggcctcttc
      361 tatgtacaga ccccgctgca ctctatcaac cagctcgggc ccgacggcaa aacgccgctc
      421 atcaacccgg cgaaccaggg aaaatataca atgaccagta ccggggacgt agtgcttgcc
      481 agcgacaatt acgtgcagta cacgcccgca acggatcttt ccgtcgtcgg tcacgcctac
      541 ccggacttca cgtcctcgct gatcaaccgc ttcacgttct ttggccggct tagcctctcc
      601 ttccaattcg attggatcca cggcaacagc atctacaacg taacaaaaca gtggctgtac
      661 cggcccactg gaggaacggg cggacaaggt ggcgaatcga gcgacctgga caagaagctg
      721 accatcgccg gccagaccgg atcgtttgtc aactactaca actcgctgta taacgttggc
      781 ttgccggtct cgccgtttgt cgaaagcggt tcctatatcc gccttcgcga cttgtccatc
      841 ggctacgacc tttcaaggta tgtaaccggc accaaagcca tcaaacggct caccatcact
      901 gcttccggcc gcaacctgct gacctttacc aaatatagcg gtctcgatcc ggaaaatacc
      961 ggagcctacg acgagcaggg cgacgacctt tctcacagca ggacaggggc tttctccggc
     1021 gtcgactttt atggcacgcc aaaccttcgg tcctatcagt tttcgctgaa cgtcggattc
     1081 taattgatca ctcaacgcat gaaataatat gaataagcaa ttatatagcc cacgcctcat
     1141 cagacgcgcc atactgcctc tcctggcttg cacccttttg ctgggggcct gccagaagaa
     1201 gatcctggga atcctcaatc cgaatttacc caccccgcag ggatctttgg ttacggaagg
     1261 cggcatcgac gcctttgccg aaggcatctt ttacaaatgg atcgcgttcg agaccggtga
     1321 cggaaatctc aatttcttcg atattgcctg gtacatcgaa agcaatatgg gtgacgagga
     1381 tttcacgccg tactccaact acggttcgcg ttatccgatg aatatcgcca gcattacctt
     1441 gccggcgccc tacaatacgg tggtcaacaa tccgtccggc tttgccacgc agctggacat
     1501 cctccggtcc ttcaattccc ggagtgccgg cgacggcaac gccatccaat acgaatggga
     1561 ttgtttctac tacgttaatg cgcaagccaa catcctgtta aatgcgctgg acaaccctgc
     1621 gcttaagctc agtggcgatg caacgaccaa aaaggccctg ttgcaggcct gggcgtatta
     1681 ttggaaaggc tatgcctatt ccaagctggg ttccatgtat cttgccgctg tcatcgacga
     1741 cagtccggac agcaccgtta agagcttgac cagcaacatc tatgtcgcgc atgaccaggt
     1801 gattgctgcg gccaacaaga attttaacca ggcagcctcc cttttctccg gcattacgga
     1861 agatgccgac tatgatgcga ccttcagagc aattattccc agtttcaacg cgccaaccca
     1921 ggtgatcacg ccaaccatgt gggtccacca gatcaattca tttgaagcaa gggattacct
     1981 ggtcaaccat aaggtagact cgatgaccag tgccgattgg aataacgtgc tgacacttgc
     2041 gaccgcggga atgcagcaag gggaccatac gctcatgtgg ggggtcgctc ctgccggcgt
     2101 gcctgatctc acccaaaatc taggattctt gttccatccc atgatgctgc acaccccgag
     2161 cggcgccctt tctttcgtca gcgaacggtt gatccaggat atccaacccg gagacgcccg
     2221 cttgaattac tttgaaccgt atcccacggg aaccgttgtc aatgtgctca accgcggcat
     2281 ccagtttgga acccgatacg cgcccgttga tatcgaaaat ggcggccgct atgctacgga
     2341 gaacttctcg tctcaggccc tcgttcccat tgccccgacc tgggaagaga atgccctcat
     2401 gatcgcggag tgcaatatct atctgggcaa cgtcgacgcc gggttacagc tgatcgacca
     2461 ggtccgggca tcacagggcg ctggcctgcc ggtgctggcg ggctcaggga tgtcccagga
     2521 ctcggcgatt gcgcagctcc gcagcgaacg ccggatcggc ctctatctcc acaacgtctc
     2581 gtggtatgat gcccgtcgct ggggtattac cgctcccgcg gcgcaaggcg gtggcaggat
     2641 aggaaatatt gtggtacccg gaagccttat cggcgccaac agttaccagg tgcttccctg
     2701 cttcatcaat tattcttttt ccgactattg ggacgtgccg aagaacgaaa cggacttcaa
     2761 tccgccggcc gcaggctcgg cgccggttgc gaattaaccc ggaaccgtcc accttacaac
     2821 cgtttcacac aacaataaaa cccggtcttt ggccgggttt cttgttaccg gtctttcccg
     2881 gatcctactg gaagctcagc gtaacgtgac cactgctgcc tcgcactttt atgggtatcc
     2941 ctccgccatt caatgttccc tcgatgctgt gttcatccat cttgcccgaa aaattgctca
     3001 atttatccgt gtggacccgg tccccggaga tcgccagatt gtaaccctgc ccggatggca
     3061 cagaaaggtg gatactgccg ccggaatttt ccaactccag gaatttaccc atcgatttca
     3121 tggtgacatc gatattcccg cctgaagtcg aagcattcag actgcagcgc atgtcatcaa
     3181 ggtggatgct gccgccggac gtagaggtcc tcagatctcc ggcgatctca tcaccgtcga
     3241 tatcgccgcc tgaagttgtc gcatgcaccg tccccttgac attctggagg cgcaaatttc
     3301 cgccgg
//
LOCUS       DATGKE010000086         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1324707, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000086 DATGKE010000000
VERSION     DATGKE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324707"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VKU83_01520"
     CDS             complement(<1..186)
                     /locus_tag="VKU83_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92251.1"
                     /translation="MSRLSSVILCIISSTFLFSCSRKNYVPFTRDLRSKLEKENIDLR
                     QVQFYVDQKLILSRNMGD"
     gene            372..1628
                     /locus_tag="VKU83_01525"
     CDS             372..1628
                     /locus_tag="VKU83_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92252.1"
                     /translation="MLKNFFKVAVRNLWRQRGYSFLNIFGLAVGMACGLLILLWIRDE
                     EAVDNYHVKGDRLYSVYERQYYDGKIESGHFTPGVLPDEMKRVLPEVEMACGFNAGDR
                     MTFQVGNKILKEDGAEAGADFFRMFSYPLLAGDASTALASPAGLAISRKMAIAFFGSP
                     EAAIGKAIRNENRRDLTVTAVFEDLRSTTSQKFDYVMNWHAYLDDNTWAKDWGNNGPE
                     TLLLLRKDAVASRFDKEITHFLDKYNKYQSKSFRIELGIQRFGDRYLHSQIVNGKFVG
                     GRIEYVKLFSLVAIFILCIACINFMNLTTARSVKRAKEIGIRKVVGAVRPALIRQFMG
                     EAILLAFISILLAVGIVLLALPAFNQLTGKEIGFPFGSGSFLLSLLALTVFTGFLSGS
                     YPALFLSSFQPIRVLKGTLRFSPTTA"
     gene            1659..2744
                     /locus_tag="VKU83_01530"
     CDS             1659..2744
                     /locus_tag="VKU83_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92253.1"
                     /translation="MLSIVLIIGTMVVSGQVDYVQSVNLGYNRENLIYIPLEGDLTAK
                     YALFKDQALQMPGIGEVSRGSQSPTNIENGTLGVDWDGKDPNNTIMFTNEAAGYDFAK
                     TMGLQILSGRDFSPAFGTDTVAYILNEEAAKRLAYKDPIGRRLTFWGKKGTIIGVVRN
                     FHFNSLHEPIRPLIIRFGEKDDYGNALVRTKPGQTKAALKSLETLCRELNPKFPFTYD
                     FADEQYRNLYKSEQVVHGLANCFAALAIFISCLGLLGLAMFTAEQRTKEFGIRKVLGA
                     KVGTLFTLLSKDFLLLVLFAFILAIPLAGWAMQRWLDNFVYRIGLEWWVFALAGALAL
                     LIALATVSFQAVKVALANPVKSLRTEG"
BASE COUNT          618 a          684 c          845 g          668 t
ORIGIN      
        1 gtctcccatg ttccgggaca agatcaattt ctgatcgaca tagaactgga cctggcggag
       61 gtcgatgttc tccttttcga gtttgctgcg aaggtcgcgg gtaaagggga cgtagttctt
      121 tctgctgcag ctgaacaaaa acgtcgaaga aataatacaa aggatcacgg aggagagtcg
      181 gctcataaaa atgttttagt gcctcgtttg actttttgaa aacgaaatag ttatttcaag
      241 tggcgataat agttgttatc aaaatgttaa aatggcggcg cccgccgcct gctgccgctg
      301 gcagggccgg ctgcttgctg caacatttac ccggtttgcg cgtccagtag gtatcatcaa
      361 atcccgcgtt catgcttaaa aatttcttca aggtggctgt ccgcaacctc tggcggcagc
      421 gaggctatag ttttctaaac attttcggac ttgcggtggg aatggcatgc ggtcttctta
      481 ttcttttatg gatacgggac gaagaagcag tcgacaacta ccacgttaag ggtgaccgtt
      541 tgtatagcgt gtacgagcgg caatattacg acggcaagat cgaatcgggc cattttaccc
      601 ccggcgtgct gccggacgag atgaaaaggg tcttgcccga ggtggaaatg gcttgtggat
      661 ttaacgcggg cgatcgcatg accttccagg tcggaaataa gatcctgaag gaggacggcg
      721 ccgaagcggg cgccgatttc ttccgcatgt tcagctatcc tttgctggcg ggggacgcct
      781 ctacggcact ggcttcgcct gccggtctgg cgattagccg gaagatggcg atcgcgtttt
      841 tcgggagccc ggaggcggcg atcgggaagg cgatcaggaa tgagaacagg cgggatttga
      901 ccgtgacggc ggtattcgag gatcttcggt caacgacgtc gcagaagttc gattatgtga
      961 tgaactggca tgcctacctg gacgataaca cctgggcgaa ggattgggga aacaatggtc
     1021 ccgagacgct tttgctcttg agaaaggacg cggtcgctag ccgcttcgac aaggagatca
     1081 cccatttttt ggataaatac aacaagtatc agtccaagag tttccgcatc gagctgggca
     1141 ttcaacggtt cggggacagg tacctgcata gccagatcgt gaatgggaag ttcgtcggcg
     1201 gccggattga atacgtgaag cttttcagtc tggtggctat tttcatcctc tgtatcgcgt
     1261 gcatcaactt tatgaatctg acaacggcgc gttccgtcaa gcgggcaaaa gaaattggca
     1321 tccggaaggt cgtgggggct gtgcggccgg cgttgatcag gcaattcatg ggggaggcaa
     1381 tcctcctggc gttcatctcg atcctgctgg cggtaggaat cgtgctcctg gcgttgccgg
     1441 cattcaacca gctgacgggt aaggaaatcg gtttcccgtt tggttccggt tcgttcttgc
     1501 tgagcctcct tgcgttgacg gtatttacgg gattcctctc cgggagttat ccggcccttt
     1561 tcctttcctc gtttcagccg atccgcgtgc tgaaaggtac cctgaggttc agccccacga
     1621 cagcctgatt tcgaaagggc ctggttgttt ttcaattcat gctgtcgatc gtattgatca
     1681 tcggaacgat ggtggtatcg ggccaggtag attatgtgca atcggtcaac ctcgggtata
     1741 accgcgagaa cctgatctat atcccgcttg agggcgacct gaccgccaaa tatgcgctgt
     1801 tcaaagatca ggcgctgcag atgccgggaa tcggcgaggt cagccggggc tcacagtccc
     1861 cgaccaatat tgaaaacggg acgcttggcg tcgattggga cgggaaggac ccgaacaaca
     1921 cgatcatgtt cacgaatgaa gcggcggggt atgatttcgc taagaccatg ggcttgcaaa
     1981 tactgtcagg acgcgatttc tcgccggcct tcggcacgga taccgtcgca tatatcctga
     2041 acgaagaggc tgcgaagcgg ctcgcgtaca aagacccgat cggcaggcgg ttgacctttt
     2101 ggggcaagaa gggaacgatc atcggcgtgg tccggaactt tcatttcaat tcactgcacg
     2161 aaccgatccg gccactgatc atccgctttg gcgaaaagga tgactatggg aacgccctgg
     2221 tcaggacaaa gccggggcag acaaaggcgg cgttgaaaag cctggaaacg ctgtgccggg
     2281 aactcaaccc gaaatttccc tttacgtacg atttcgcgga tgagcagtac cggaatctgt
     2341 acaaaagcga gcaggtggtg catggcctcg cgaattgctt tgcggcattg gcaatcttta
     2401 tctcctgtct cggcctgctc ggtctggcga tgtttaccgc ggagcagcgg acgaaggaat
     2461 tcggcatccg aaaagtccta ggcgccaagg taggcacgct atttacgttg ttgtccaagg
     2521 atttcctgct cctcgtattg ttcgcattca tccttgccat tccgctggca ggatgggcga
     2581 tgcagcgatg gctcgataat ttcgtctatc ggatcggcct ggagtggtgg gtgttcgccc
     2641 tggcaggggc ccttgcattg ttgatcgcgc tggcgacggt gagtttccag gcggtcaagg
     2701 tcgcgttggc gaatccggtc aaaagcctgc gcaccgaggg gtgacacgaa cagagaatga
     2761 aaagacaagt tctttgatgc tgtacattag atggcggtgg gcccgccgga gccgg
//
LOCUS       DATGKE010000087         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_83884, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000087 DATGKE010000000
VERSION     DATGKE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83884"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VKU83_01535"
     CDS             complement(<1..297)
                     /locus_tag="VKU83_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLI92254.1"
                     /translation="MATTKFSKETYLYWYELMQLIRQFELSAEEKYKMEGKIRGFFHA
                     YVGQEAIAAGCMTATRPEDPFITAYRDHGLALSKGVTANACMAELYGKATGAAKG"
     gene            complement(448..2013)
                     /locus_tag="VKU83_01540"
     CDS             complement(448..2013)
                     /locus_tag="VKU83_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI92255.1"
                     /translation="MRKNLITTLLLAFLVPVLAPAQDLPAENLDTATLSRIRQEAFEH
                     SQIPWIAHEITDVAGPRLTNSPGWHNAAEWIVKTLKDWGLSKASIEPWGQFGYGWSAE
                     KTSLAMRTPYYSPLIAYAIPWSGSTHGPVSAPAFLVEHMDSAWVTDHLAEMKGKILLI
                     AVRDTTNRPRFKPDAERYSDSALAALGDMYMMSKEEIAQIIPFFLKMRAVEDMVQHSG
                     AVAVLTSNGGRDGTVFVQSLTGYRKKDQPELPGLEVSHEDLLRLQRLVEDGHQVTLEL
                     QSDTKLYDRDLEGHNVVAEIPGTDPALGSQLVMLGGHLDSWSAATGATDNGAGCIVAL
                     EAVRILRSLGIKPKRTIRIALWDGEEEGLLGSFHYIKNHFGDPADMKLKPEQARVSAY
                     FNLDNGTGRIRGIFAQGNEKAADIFRQWLVPYKDLGATTVTLHNTGSTDHLGFDAVGI
                     PGFQFIQDPIDYETRTHHSNEDNYDHLMIGDLQQAAAIMAGFVYNTAMRAEMLPRKPL
                     PKPEKFIFDDLIP"
BASE COUNT          488 a          603 c          630 g          524 t
ORIGIN      
        1 ccctttcgcc gccccggtcg ccttcccgta caattcggcc atgcaggcat tggcggtaac
       61 gccctttgaa agggccaggc cgtggtcacg ataggcggta ataaagggat cttcagggcg
      121 ggtagcggtc atacagccgg ctgcaatggc ttcctgtccc acataagcgt gaaaaaaccc
      181 ccggatcttt ccctccattt tatacttttc ctcggcagac agctcgaatt gccggatgag
      241 ctgcatcagc tcataccaat atagataggt ctcctttgaa aacttagtgg tagccacaat
      301 caaatatttt tttaaaccgg gccacaaggt ccggttggcc ggaggccaga catcaaatgc
      361 tcgcgctcgc gggctacgtt aaaaaacaga ccgcaggcgc cggaatcgcg ggtgcaaata
      421 tacggcgaaa accgattgga tagcgcccta aggaataaga tcgtcgaata tgaacttttc
      481 gggttttggc agcggttttc ggggtaacat ttccgcccgc atggcggtgt tatagacaaa
      541 gccggccata atggcggcag cttgctgcag atcgcctatc ataaggtgat cgtaattatc
      601 ctcgttgctg tggtgggtcc gggtctcata gtcgatcgga tcctgaatga attggaagcc
      661 gggaatgccg actgcgtcaa agcccaggtg gtcggtcgac ccggtattgt ggagggtaac
      721 cgtggtcgcc cccaggtcct tgtaggggac aagccattgt ctgaagatat cggccgcctt
      781 ttcgttgccc tgggcaaaaa tacccctgat ccttcccgtg ccgttatcga gattgaaata
      841 ggcagacacc ctggcctgtt cgggtttgag cttcatatcg gcggggtcgc cgaaatggtt
      901 tttgatataa tgaaaagagc ccagcagccc ttcttcctcg ccgtcccaaa gggcaatacg
      961 aatagtccgt ttcggtttga tacccagcga acggaggatg cggacggctt ccagggccac
     1021 aatgcatccg gcgccgttat cggtggcgcc ggtagccgcc gaccaggagt caaggtgccc
     1081 gccaagcatg accaattgcg aaccaagggc aggatcggta ccgggaattt ccgcgacaac
     1141 attgtgccct tcaaggtccc tgtcgtatag tttggtatcg ctttgcagtt ccagcgtgac
     1201 ctgatgcccg tcttcgacga gccgctgaag ccgaagcagg tcttcatgtg aaacttccaa
     1261 gcccggaagc tccggctggt ccttctttct gtagccggtg aggctttgaa cgaagacggt
     1321 gccatcgcgg ccgccgttgg acgtcaggac ggcgacagcg ccgctatgct gcaccatatc
     1381 ctctaccgct ctcatcttta ggaaaaaggg aatgatctgc gcaatttctt ctttgctcat
     1441 catatacata tcgccgagcg ctgccagcgc gctgtcggaa taacgttcgg catccggctt
     1501 gaatcttggc ctgttcgtcg tatcccgtac ggcaataagc aagatcttcc ccttcatttc
     1561 cgcgagatgg tcggtgaccc atgccgagtc catatgttcg accaggaagg caggggcgct
     1621 caccggtccg tgggtactgc cgctccaggg aatggcatag gcgatcaggg ggctataata
     1681 cggcgtacgc atggcaaggc tcgtcttttc ggcgctccag ccgtagccaa actgccccca
     1741 cggctcgatc gaggccttgc taagacccca gtccttcagc gttttgacaa tccactccgc
     1801 cgcattgtgc cagccgggag aattggttag cctcggtccc gcaacgtcag tgatctcgtg
     1861 agcgatccag gggatttggg aatgttcgaa cgcttcctgg cggatgcggc tgagggtggc
     1921 ggtgtcaagg ttttcggctg gaaggtcctg ggccggggca aggacgggga ctaaaaaagc
     1981 gaggagcagg gttgtaatca ggttttttct catataactc tgttttggcg gcagctaaga
     2041 ttctgtttag gcgaaaagat actaatttac agggaattcg aacttgttgc tccgaataaa
     2101 ggatatatcc ctcgtccagt tcaagaacta ttcccaggcc aggttccgat ttcaggatcg
     2161 gatcgtcgga atttgcggca acaacggcgt cggcaagacc aacctgttgg acgcccttta
     2221 ttacctatgc tttaccaaga gctat
//
LOCUS       DATGKE010000088         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_394460, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000088 DATGKE010000000
VERSION     DATGKE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394460"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VKU83_01545"
     CDS             complement(<1..378)
                     /locus_tag="VKU83_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92256.1"
                     /translation="MMKLSPCRYLFPLLLLFAQRCDAQDNYEIQVYASPTVGKDTTMV
                     ELHSNFSLIGNTAADGVVSTNHMDRETIEITHGWNSWFETGFYIFNDIGDMGRTNYVG
                     THIRPRVMAPANWNWPVGASISFE"
     gene            513..3008
                     /locus_tag="VKU83_01550"
     CDS             513..3008
                     /locus_tag="VKU83_01550"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92257.1"
                     /translation="MKKLIVLLLSMSCFCGVKAQHAETSHPTVRITGKIIDSATHQPI
                     QYATITLYSAKATKAVGGMMTGNKGEFAIEAPRSGSYTLTIECIGYKTRRIGPFPGEE
                     RKTGLGDIPLAIHATEQQAAVVTAPRGLIENKLDKIVYNAEKDITSIGGVATDVLKKV
                     PMVSVDVDGNVDIMGNTNILFLINGRPSSIFGNNLSDALQSIPASQIKSIEVITSPGA
                     KYDAEGTGGIINIILKDNRINGVNGNLSLTGGSRLQNGSFNFNARHGKFGLNSFFSGN
                     AQLPSTTLNSSNRNSYDSTGEMLGSLSQDGSSRFVRNGYETGLNLEWDPSKYDVLSGG
                     LQYNSFGNNNRGSYLQSQTDYAPPATAPADQINSLVTNNSQFRARSLDWNLRYKRTFP
                     VEDRELDFSVDGTYGHNTASYGQLQSLPAGDSAYEGSTGSSSGHDRETNIRLDYTEPF
                     GEKVRLETGARVQLRQITSNSPVYSYDEETGLYPYNPSQSDSLTYNRHVYAGYVSMTF
                     PAFHFLDVKAGMRFERTTTAASFSEVPDTYIPPYNSWVPSIILSHSFPHDQTLKISFA
                     RRLERPDYRTLNPYTNASDPKNLSRGNPLLQPEDAYNFELGYAKSFDQGSALNVVAFY
                     HRSDHDIQPFIQYYSSFTLGDSVYTDVAVTTPMNIGSENNYGMNIYGSVPVGKKLNLR
                     TNISVFDRYIVTGTLGGPPISSFNYRVNLNASYQLSPTFIAEFFGNFRSARNEIQGRY
                     PSFTTYNVAMRKQFWNKKGSIALTVTNPIGKYVNQETAVKGAGFTLNSLRQIPFRSIG
                     INFTWKFGKLEFKKDKDDQQKDIPTTPEGPGGN"
     gene            complement(3024..>3243)
                     /locus_tag="VKU83_01555"
     CDS             complement(3024..>3243)
                     /locus_tag="VKU83_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92258.1"
                     /translation="RPALMKAVPGQRNLNKYYRYFAWIYPIGRALFPGIFCTLQELAL
                     AMIHATAAGAKPILEVRDIVNLSRERPN"
BASE COUNT          824 a          894 c          825 g          700 t
ORIGIN      
        1 ctcgaaggaa atgctcgcgc ctaccggcca gttccagtta gcgggcgcca tcaccctggg
       61 ccggatatgg gtgccgacat agttcgtccg tcccatatcg ccaatatcat tgaagatgta
      121 gaagccggtt tcgaaccagg aattccagcc atgggtgatc tcaattgtct cccggtccat
      181 atggttggtc gacactacgc catccgcggc agtattgccg atcaaactaa agttgctgtg
      241 cagttcgacc atcgtcgtat ccttgccgac tgtcggcgaa gcataaacct ggatctcata
      301 gttgtcctgg gcgtcgcaac gctgcgcgaa taacaataac agcgggaaga ggtaccgaca
      361 gggagaaagt ttcatcatac gcaggcctta aagatcggaa aattatctgt tttgaatctg
      421 aaaattcagc aggaaaacac tcaggaataa atgagtgatt caggttttcg ccagattatc
      481 gaatgaattt tgccgttttg cagactgaaa tcatgaagaa attaatcgtt cttttactgt
      541 caatgagctg tttctgcggg gtgaaggcgc agcacgcgga gaccagccat cccactgtca
      601 ggataaccgg caagatcatc gattccgcta cccaccagcc gatccaatac gccaccatta
      661 cattatattc ggcgaaggcc accaaggcag tcggcggaat gatgaccggg aataagggag
      721 aattcgctat cgaagcgccg cgatcgggtt cctataccct gaccattgag tgcatcggct
      781 ataaaacccg ccggattggc ccgtttcccg gtgaagaaag aaaaacaggg ctgggcgaca
      841 ttccccttgc catccacgcc acggagcagc aagcagcggt ggtcaccgcc ccccgcggcc
      901 tgatcgaaaa caaactggat aagatcgtgt acaacgccga gaaggacatt acctctatcg
      961 gcggcgtagc gacggacgtg ctgaagaaag tcccgatggt ctcggttgac gtcgacggca
     1021 atgtcgatat tatgggtaat accaatattc tctttttgat caacggcagg ccatcgagta
     1081 tttttggtaa caacctcagc gacgctttgc agtcgatacc ggcaagccag atcaaaagca
     1141 ttgaagtgat caccagccct ggcgcaaaat acgatgcgga agggacgggc ggcatcatca
     1201 acattatcct caaggacaac cggatcaatg gcgttaatgg aaaccttagc ctgaccggcg
     1261 gatccaggct ccagaacggt tcctttaatt tcaatgcccg gcacggaaaa ttcgggctga
     1321 actcgttctt tagcgggaat gctcagcttc cttcaactac actgaattct tcaaaccgca
     1381 actcgtatga ttcgacaggg gaaatgctgg gcagtctaag ccaggatggc agcagtcgct
     1441 ttgtgcgaaa tggatacgaa accggcctga acctcgagtg ggatcccagt aaatatgacg
     1501 ttttgtccgg cggcttgcag tataacagtt tcggcaacaa taaccgcggc tcgtatctgc
     1561 aatcgcaaac agactatgcg ccaccggcta ccgctccagc ggatcagatc aacagcctgg
     1621 tcaccaataa cagccagttc cgggcgcgct ccctcgactg gaacctgcgt tataaacgga
     1681 cttttccggt agaggatcgg gagctcgatt tctccgttga cggcacctat ggtcacaaca
     1741 cggcatccta cggtcagctc cagtccctgc cggccggtga ttcggcttat gaggggtcaa
     1801 ccggaagcag ctcggggcat gaccgggaga ccaatatccg cctggactac accgaaccct
     1861 ttggcgagaa ggtccggctg gagactggcg ccagggtgca gcttcgacaa atcaccagca
     1921 actcgcccgt atattcctac gacgaggaga ccggcctgta tccgtacaac ccttcccagt
     1981 ccgactctct tacctacaac cgccacgtat atgcgggata cgtctcgatg acctttcccg
     2041 cttttcattt tctggatgtc aaggccggaa tgcgttttga acggacgacg accgcagcga
     2101 gcttttccga agtacctgat acctatattc cgccctataa cagttgggtg ccttccataa
     2161 tcctgtctca cagctttcct catgaccaga cgctcaagat cagtttcgcc agaaggctgg
     2221 aacgacctga ctatcgtacg ctgaaccctt acaccaatgc gagcgatccg aagaacctgt
     2281 ctcgcggcaa cccgctgctg caacccgagg acgcatataa tttcgagctg ggctacgcga
     2341 aatccttcga ccagggaagc gcgctgaatg ttgttgcctt ctatcaccgg tcggatcacg
     2401 atatccagcc attcattcaa tactatagca gcttcacgct tggcgactcc gtttacacgg
     2461 acgttgctgt gacgactccg atgaacatcg gatccgagaa caactacgga atgaatatct
     2521 atgggtccgt ccctgtcggc aaaaaattga acctgcggac gaatatttca gtctttgacc
     2581 ggtatatcgt gactggcact ctcggcgggc cgccgatcag cagcttcaac tatcgggtca
     2641 acctgaacgc atcctatcag ttgagtccca ccttcatcgc cgaatttttc ggaaatttcc
     2701 ggtcggcaag gaacgagatc cagggccggt acccctcttt cacgacctac aatgtcgcca
     2761 tgcggaaaca gttctggaac aaaaagggca gtattgcttt aaccgtgacg aacccgatcg
     2821 gcaaatacgt taaccaggaa accgccgtga aaggggccgg cttcacgctg aacagccttc
     2881 gtcagatccc cttccggtca attggcatta acttcacatg gaaattcggc aaactggaat
     2941 tcaaaaagga caaggatgat cagcaaaagg atatcccgac cactccggaa gggccaggcg
     3001 gaaattaacc cgtcgccgcg gatctaattc ggacgctcgc gtgacaggtt gacaatatcc
     3061 cggacttcga gaatcggctt agcgccggcg gcggtcgcgt ggatcatcgc aagagccagc
     3121 tcctgcaacg tacagaagat tccggggaag agtgcgcgcc cgataggata tatccaggcg
     3181 aaatagcgat aatatttatt gaggtttcgc tggcccggaa ccgccttcat taaggcaggg
     3241 cga
//
LOCUS       DATGKE010000089         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1119444, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000089 DATGKE010000000
VERSION     DATGKE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119444"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="yidC"
                     /locus_tag="VKU83_01560"
     CDS             complement(<1..1388)
                     /gene="yidC"
                     /locus_tag="VKU83_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HLI92259.1"
                     /translation="MGMDRNTVIGFVLIGVLLLIYLVTSTKATHELEAQRQRAQDSIA
                     AVKAKQAALAQGKDTVAVKAPAADSTLTGFNRAARGTERFTTIENEVIKVVFSNKGGQ
                     PVAVTLKQYKAYDSNLVKLIPPGSTNRLMYNISTGQNMTADIADLYFDDPQVVRNGDG
                     SQTIRYRLPTAAGESLVHEFTIRPNDYLVDWNIVIGNPAALFYQNSFVLSWLTQLDKT
                     QQDVRYERRQASVSYLEDGRFDYMVAKTEKTLDKPVQWVAVSQQFFNTALIAKNGWSS
                     GEIHLAKETSDTSNVVERAQINLQAKIPALAQTVLPMQIYYGPNDFHILAHQAEGMTK
                     IIDLGRGMYSFVRPINVYVIMPVFNFFKSHIASYGLVILLLTFFIRLFTSPLVYSSYL
                     SGAKMKALRPEIEAMKKRVGNDQQQVGMEQMKLFREAGVNPLGGCIPALLQIPIFFAL
                     YSFFNTYMGLRG"
     gene            complement(1492..>2168)
                     /locus_tag="VKU83_01565"
     CDS             complement(1492..>2168)
                     /locus_tag="VKU83_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLI92260.1"
                     /translation="VVNVHSEFITDENVAEKLGNLDGLLVAPGFGHRGVEGKITAVKY
                     ARENRLPFFGICLGMQMAVIEFARNILGWKDAHSTEMNATTAYPVIDLMEEQKKVSIK
                     GGTMRLGSYPCLLKDGSLAHRIYDGRTVISERHRHRWEFNNAYLRQFEDAGMLPSGTN
                     PESGLVEIVELPSHPFFIGVQYHPELKSTVENPQPIFVHFIRAAKEFAEAKNSVKNPL
                     LQSELI"
BASE COUNT          464 a          629 c          539 g          536 t
ORIGIN      
        1 tggccgcgca aacccatata ggtgttgaaa aaactgtaca gtgcaaagaa gatcggaatc
       61 tgcagcaacg cgggaataca tccacccagc ggattcaccc cggcttcgcg gaatagcttc
      121 atctgctcca ttccgacctg ctgttggtcg ttccctaccc tctttttcat tgcctcgatt
      181 tccggccgca gcgctttcat cttcgcaccg ctcaggtagc tgctgtaaac aagcggcgag
      241 gtaaagagcc ggataaaaaa cgtcagcaac aggatcacca gcccatagct cgcgatatgc
      301 gatttgaaaa aattgaatac cggcatgatc acgtagacat tgatgggccg gacaaacgag
      361 tacattcctc ttcccaggtc gatgatcttc gtcattccct cggcctgatg cgccaggata
      421 tgaaaatcgt tgggaccgta atagatctgc atgggcagga ctgtctgcgc caaggcgggg
      481 atcttcgcct gcagattgat ctgcgcgcgc tccaccacgt tcgaggtgtc cgacgtttcc
      541 ttcgccaggt ggatctcgcc tgagctccaa ccgttctttg cgatgagcgc cgtgttgaag
      601 aactgctggg acaccgccac ccattgcacc ggtttgtcaa gggtcttttc cgttttcgcg
      661 accatataat cgaaccggcc gtcttccagg tagctcacac ttgcctgccg ccgttcataa
      721 cggacgtcct gctgcgtctt gtccaactgt gtcagccagg agaggacgaa gctattctga
      781 tagaacaacg ccgccgggtt accgatcacg atattccagt ccacgaggta gtcatttggc
      841 cggatcgtaa actcgtggac cagcgattcg ccggccgccg tcggcaagcg gtagcggatg
      901 gtctgactcc cgtcgccatt tcggacaacc tgcgggtcat caaaataaag gtcggcgatg
      961 tcagcggtca tattttgacc ggtgctgata ttgtacatca gccggttcgt gcttcccggc
     1021 gggatgagct ttaccaggtt gctgtcgtac gccttgtatt gttttagcgt taccgcgacc
     1081 ggctggccgc ccttattgct gaaaactacc ttgatgacct cattttcgat ggttgtgaaa
     1141 cgctcggttc ccctggctgc cctgttgaaa ccggtcagcg tgctatccgc ggccggcgcc
     1201 ttgacggcta ccgtgtcctt tccttgcgcc agcgccgcct gtttcgcctt cactgctgca
     1261 atactgtctt gagcccgctg gcgctgagct tccagttcat gagtggcttt cgttgacgta
     1321 accagataaa taagcaatag aacccctatc agcacaaacc caatgaccgt attgcgatcc
     1381 attcccatga tacttttttt aggaagtgca aatataggtc attgtggtct accacgacta
     1441 tccggccggc caaaccggcg atttattcct ccggaaagcc ttaatgaccg gctagatcaa
     1501 ctcactctgt aacagcggat tcttcactga attctttgcc tcggcaaatt ccttggcggc
     1561 gcggatgaaa tgcacaaaga taggttgtgg gttttcgacc gtgctcttta gttcgggatg
     1621 gtattgaacc ccgatgaaaa aagggtggga aggcagttcg acgatctcca ccaggccgct
     1681 ttcggggttt gtcccgctcg gcagcatccc ggcatcctcg aattgccgta aataagcgtt
     1741 attgaattcc cagcgatggc ggtgccgctc gctgatgact gtacggccgt catagatccg
     1801 gtgtgccagg ctcccgtctt tcagcagaca gggataggaa cctaaccgca ttgtccctcc
     1861 cttgatcgac acttttttct gttcttccat cagatcaatg accggatagg ccgtggtggc
     1921 attcatttcg gtggaatgcg catccttcca gccgaggata ttcctcgcaa attcgatcac
     1981 cgccatctgc attcccaaac aaattccaaa aaaaggcaac ctgttctccc gcgcatattt
     2041 caccgcggtg atcttaccct ccacgccgcg atgaccaaac cccggcgcaa ccagcaggcc
     2101 gtccaggtta cccaactttt ccgcaacgtt ctcgtcggtt atgaactcgc tatgaacgtt
     2161 cacgacct
//
LOCUS       DATGKE010000090         5544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_490181, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000090 DATGKE010000000
VERSION     DATGKE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490181"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VKU83_01570"
     CDS             complement(<1..613)
                     /locus_tag="VKU83_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLI92261.1"
                     /translation="MISFLFYALAACSLPVGTGAAGATGPRELTVIDTAGMRREIMEV
                     YRREPDASFAVAFEDISTGQQLLLNPHTLFHAASTMKTPVLVEAYRQVASHRLSLNDS
                     LSIHTDFRSIVDGSRYELDSSTDSETDLYRRAGQKMSLRDLLFQMITQSSNLSTNLVI
                     EKVGAKNVMATMRELGAMDIRVLRGVEDEKAFEKGMNNMTTAFD"
     gene            complement(671..3124)
                     /locus_tag="VKU83_01575"
     CDS             complement(671..3124)
                     /locus_tag="VKU83_01575"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLI92262.1"
                     /translation="MKKVLPILFVLFTQVAFSQSQTCPLNSNWSKNDLTHWWAYTGNN
                     AGGNTPGDEIQKYDSSLGAPGGTIGVSSIGEYLLPSVLGIQVLSVPSTDPYGGFPTIP
                     NINGYQYSNSLKLGSTSITHSAFSGGGGGYVRGVKYRINVPPGPTTVPYTMTYAYAMV
                     LENGTHMSNQQPLFTATLTAGDSVVTCASPKYLLPTLSNSRDSSNIGLPLDSAAAKKE
                     GFQPSLLASPNPDPNGGPNAPHLMDVWYKKWTEVTFDLSAYRGQQVVLNFETDNCVPG
                     GHFAYSYIALRNTCGGLQISGDSSACQSVTLIYSVPGLTGARYRWSVPPDWSILSGAD
                     SSVMKVQVGTDPGQVTVNEQNSCANLAASLNVVTALPTIAGAVFGGTEVCTGVNAVNL
                     TTGGNRGAVVGWLATAGGVTTVLDDTTSQYTAQNLTGTTIYRALIKNGQSCDLDTTSG
                     STVLVDPLSVGGTLTPGTFEFCEGQNKDALLQLTGQVGTPVNWQLSTDGVNWNGFVPA
                     DTATKYSLQGVITNTDYRVIVKSGVCPQDTSTIADVSYINVPFPQATYSPADTVICYN
                     TPAFLHTTITIGTNYAWDNTATLTGIGNDIVTQLPYTLSPVAKPKVTTDYVLSVENAG
                     CPNLLLDTFHVRVLPPILVDAGNDTSVVAGEPLQLNAVSNDTTSAGGDSFVWSPIIGL
                     NNPDIANPVGIYTSETDSIRYLVTATSQYGCTGTGQVVVKVFKTAPDIFVPNAFTPSG
                     PANNVFRPIPVGISTLVFFRVYNRWGQLVYSTTRMGDGWDGSVDGHPQDSGTYVWMVA
                     GITYTGKRIYHRGTMILVR"
     gene            complement(3356..4030)
                     /locus_tag="VKU83_01580"
     CDS             complement(3356..4030)
                     /locus_tag="VKU83_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLI92263.1"
                     /translation="MENSLLNGWEGRYLAYYDRLGRFADGSHDLGHFQRVWRTAKRIN
                     QSEGGPANELVLLTAAYFHDLVALPKNHPDRRDASRISAERTAALLLSEWGDFPADLV
                     QSVRHAIHAHSYSAGVEAITPEAKILQDADRMEALGAIGLARVFYTAGQLNSALFDGA
                     DPFAERRPPDDTQFALDHFDMKLLRLPALMNTAAGRKMAEERAGYLREFLARIGQEIG
                     GPSAAG"
     gene            4136..4333
                     /locus_tag="VKU83_01585"
     CDS             4136..4333
                     /locus_tag="VKU83_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92264.1"
                     /translation="MSMLKACLLAFFSGVFFFFLILQSAWWLVPGVLTWTAIFVWQDR
                     QERQRDQARGLDTEMKKTDRS"
     gene            4584..5006
                     /locus_tag="VKU83_01590"
     CDS             4584..5006
                     /locus_tag="VKU83_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92265.1"
                     /translation="MGVDSLRTIAPGAGTVILRPLRHDETIVREAFPGALDLLARALA
                     LEFGCRYLPELLTKTRPTLPNKFLTRRQRRMELEDVYRLTLPATGDGCISADTPFLLI
                     DDILTTGTTMRTLIHILQGTFPLSPIRAFTLTRAANTV"
     gene            5163..>5544
                     /locus_tag="VKU83_01595"
     CDS             5163..>5544
                     /locus_tag="VKU83_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92266.1"
                     /translation="MTIQRLLARLVSLAIICVFFTQPVFSQTKTITGKITDDKGNPLP
                     GVSVSVKGSTVGTSTDAGGNFMLNVKSSAKTLVLSSVGYSDMEVDISSQTTIEVKMTA
                     SSTTLGDLVVTGYGTARKKDLTGAV"
BASE COUNT         1216 a         1569 c         1456 g         1303 t
ORIGIN      
        1 ggtcaaaggc ggtggtcatg ttgttcatgc ccttctcgaa tgctttctca tcctcgaccc
       61 cgcgcaacac gcgaatatcc atcgcaccga gctcccgcat ggttgccatc acattcttag
      121 cacctacttt ctcgatcacg aggttcgtcg acagattgct gctctgagtg atcatctgga
      181 acaacaggtc gcgcagcgac atcttctgcc ccgcccgcct gtacaggtct gtttcactgt
      241 cggtagagga atcgagttcg taccggctgc cgtccacgat gctccggaag tcggtatgga
      301 tcgacaggga atcgttcaat gacaaccggt gcgacgccac ctggcggtac gcctccacga
      361 ggaccggagt cttcatcgta ctggcggcat gaaataaggt atgcgggttc agcaatagtt
      421 gctgccctgt cgagatatcc tcgaaggcca ccgcaaagct tgcgtcaggc tcgcgccggt
      481 aaacttccat gatttcccgg cgcattccgg cagtgtctat caccgtaagc tcgcgcgggc
      541 cggttgcacc ggccgcaccg gtacccaccg ggaggctgca cgctgccagc gcataaaaca
      601 gaaaactgat cacgattgaa ttggtttgcc cataagatag gcaatttttg aaaaccgcgt
      661 tccagccgct ctaccgcacg aggatcatcg ttccccggtg atagatccgc ttcccggtat
      721 aggttatgcc tgccaccatc cagacgtagg taccgctgtc ctggggatgg ccgtcgacac
      781 tcccatccca cccgtctccc atccgcgtcg tgctgtagac aagttgcccc catctgttgt
      841 acacgcggaa gaagacgagg gtcgaaatac ctaccggtat cgggcggaag acgttgttgg
      901 cggggccgga gggtgtaaag gcattgggaa caaatatatc cggggcggtc ttgaaaacct
      961 ttaccactac ctgcccggtg cctgtgcagc catactgcga cgtagccgtg accagatacc
     1021 ggatcgaatc tgtttcggac gtatagatgc caacggggtt ggcaatatcc gggttattga
     1081 ggccgataat gggcgaccat acaaagctgt ctcctcccgc cgaggtcgta tcattcgaaa
     1141 ctgcattcaa ctggagcggt tcgccggcga ccacggacgt gtcattgccg gcatccacga
     1201 gaataggcgg aaggacccga acgtggaaag tatcgagcag caggttcgga cagcctgcat
     1261 tctcgacgct tagcacatag tcggtcgtga ctttcggctt tgcgacgggc gaaagcgtat
     1321 agggcagttg cgtaacgata tcgttgccga tacccgtcag cgtggccgta ttgtcccagg
     1381 cataatttgt cccgatcgtg atcgttgtat gcagaaaagc aggcgtatta tagcaaatga
     1441 ccgtgtccgc cggcgaatac gtcgcttgcg ggaacggaac atttatataa ctgacgtcgg
     1501 cgatggtgct tgtgtcctgt ggacagacgc cgcttttcac gataacccgg taatcggtgt
     1561 tcgtgatgac cccctgcaga ctgtattttg ttgccgtgtc cgccggaacg aatccgttcc
     1621 agttgacccc atccgtcgaa agctgccagt tgaccggagt gccaacctgg cccgtcaact
     1681 ggagcagggc atccttgttc tgtccctcgc agaattcaaa tgtcccaggc gtcaacgttc
     1741 cccctacgct taaaggatcc acgagcacgg tggaccctga ggtcgtatcc agatcacagg
     1801 actggccgtt tttgatcagc gcccggtaaa tcgttgtacc cgttagattt tgcgcggtat
     1861 actgcgaggt ggtgtcatca agtaccgttg tcaccccgcc ggccgtcgca agccatccaa
     1921 cgaccgctcc ccggttacct cccgtcgtca ggttcactgc gttgactcct gtacatacct
     1981 ccgttccgcc aaagacggca ccggcaatcg ttggcaaagc ggtaacgaca ttcagcgatg
     2041 cggccaggtt ggcgcaactg ttttgttcat tcaccgtaac ctgtccgggg tcggtaccga
     2101 cctgcacctt cataacgctg ctatccgcgc ccgacaagat cgaccagtcc ggcgggaccg
     2161 accacctata cctggcgccg gtcagcccgg gcactgaata gatgagtgta acgctctgac
     2221 acgcagagct gtcgccgctg atctgcaggc ctccgcaggt atttcgcaaa gcgatatagg
     2281 aataggcaaa atgaccgccg ggcacgcaat tatcggtttc aaaattcagg acgacctgtt
     2341 ggccccggta ggcggaaagg tcgaaagtca cctctgtcca tttcttatac cagacatcca
     2401 tcaggtgcgg cgcgttcggg ccgccgttag ggtccgggtt gggcgaggcc aggagactgg
     2461 gctggaatcc ttccttcttg gctgccgccg aatccagcgg caggccgata ttgctgctgt
     2521 ccctcgaatt ggacagggtc ggcaggagat attttgggga cgcacaggtg acgacgctat
     2581 cacccgcggt gagcgttgcc gtaaacaaag gttgctggtt ggacatatgc gttccgttct
     2641 cgagtaccat ggcgtaagcg taggtcatag tatagggcac ggtggtaggt cccggcggaa
     2701 catttatgcg gtatttcacc ccgcggacat agccgccgcc gccgccgctg aatgcggagt
     2761 gcgtaatgga agtcgagcca agcttgaggg aattgctgta ttggtacccg ttgatattcg
     2821 ggatggtggg aaacccgcca tagggatccg tcgatggcac ggagaggacc tggatgccca
     2881 ggactgacgg caaaaggtac tccccgatag agctcactcc aatcgtcccg cccggtgcgc
     2941 ccaggctgga atcatatttc tgtatttcat cgccgggggt gttcccgccg gcattattgc
     3001 ccgtatatgc ccaccaatgc gtcaagtcat tcttcgacca attgctgttt aaggggcaag
     3061 tttgagactg cgaaaaagca acctgggtga acaatacaaa taagataggg agaacttttt
     3121 tcattggtct acgataccgt aaaattaaac atttccaccg gcccttcctc ctcatagatt
     3181 ctcaataaga tacgaggtaa accgcggttg agttgactgg cccgaaacgt attttcgaag
     3241 tcactccctg ctcgctgata aacaattgca gatcaaccta aatgcaatac ccatgccgag
     3301 agcccggatt ggcccggcac gaatggctgc accctccggc tttgacctta tagggctagc
     3361 cggccgccga tggcccaccg atttcctgcc cgatccttgc caaaaactcc cgaagatagc
     3421 cggcacgttc ttcggccatt ttccggccgg ccgccgtgtt cattaaggcg ggaagcctga
     3481 gcagcttcat gtcgaaatgg tcgagcgcaa attgcgtatc atcaggtggc ctgcgctccg
     3541 caaaaggatc cgcaccgtcg aacaacgcgg aattcagctg cccggcggta taaaaaacgc
     3601 gcgccaggcc gatggcgccc aacgcttcca ttcggtcggc atcctgcagg atttttgctt
     3661 ccggagtgat tgcctccacg cctgccgagt aactatgtgc atggatggca tgcctgacgc
     3721 tttgcaccag gtccgcaggg aaatcccccc attcggacag gagcaaggcg gctgttcgtt
     3781 ccgcgctgat ccgtgacgca tcccgacggt caggatggtt cttcggcagc gcgacgaggt
     3841 catgaaaata agccgcagtg agaagcacca gctcattcgc cggtcctcct tccgattggt
     3901 tgattctttt tgccgtccgc cagacacgct gaaaatgacc caggtcatgc gacccgtccg
     3961 cgaaccgccc aagcctgtcg tagtaggcga ggtagcgccc ctcccatccg ttcaatagac
     4021 tattttccat ttataagtaa gttctattag caggaattgt cctttttttt atttcgtgcc
     4081 gacgattgtc atctatcacg ctatattatg cgtaattttg ttcagtcgca aaaagatgtc
     4141 catgctcaag gcctgtttat tagccttctt ttcgggggtt ttcttcttct tcctcatcct
     4201 gcaatccgcg tggtggctgg ttcctggcgt tttgacctgg accgcgatct tcgtctggca
     4261 ggaccgccag gaaaggcagc gcgaccaggc gcgcggtctc gatactgaaa tgaaaaagac
     4321 cgatcgcagt taggattcat ccgtagctcg tatttattcc ccttcgccac ttcctcgttt
     4381 ccgcgctttt tctccattat atctttgcgg cggggaaaac gatcatgccg ctgttacgat
     4441 catatataaa ggacgggaaa aatctttatc gtcattattt tctttgccct taccttccac
     4501 ttggagccgg cgaagatagt gtttcaaaaa gtctcctcag atttaaacag cgacttcaac
     4561 cggatctcga tcaatggatc ggattgggcg tcgattcgct gcgaaccatt gcgcctggcg
     4621 ccggcacggt catcctccgc cccctgcgcc acgacgaaac catcgtgcgc gaagcgtttc
     4681 cgggagcgct tgacctgctt gcccgggctc ttgctttgga attcgggtgc cgctatcttc
     4741 cggagctgct gacaaagacc cggccaacgc tgccaaacaa atttctaacc cgccgccagc
     4801 gccggatgga actggaagac gtctaccggc tgacgcttcc ggcaactggc gacggctgca
     4861 tttcggccga tacgcccttc ctgctgatcg acgacatcct taccacgggc acgaccatgc
     4921 gaacacttat ccacatcctt caggggacat ttccgctcag ccccatccgg gcattcacgc
     4981 tgacccgtgc tgctaatacg gtttagctaa aaatctttcg ggattgttgc aattagttgc
     5041 agcattttta caatatggtt ttaaatcgta ttcacatgag cttggctttc atcggataaa
     5101 aaaaattgtt aaatcaattt taactattta tcttagttgc atcattgcta aacttttgct
     5161 ttatgaccat tcaacgactg cttgccagac tggtaagtct tgcgattatt tgcgtatttt
     5221 tcacacagcc ggttttctct caaacaaaga ccataacagg aaaaataact gacgacaagg
     5281 gcaacccctt gcctggagta tcagtttctg ttaaagggtc caccgtaggt acctccactg
     5341 acgcaggggg caatttcatg cttaacgtaa aatcgagcgc caaaactctt gtactttcct
     5401 ccgtcggata ttccgacatg gaggtagata tctcgagcca gacgacgatt gaagtgaaga
     5461 tgacagcctc cagcacaaca ctcggtgacc tggtcgttac gggatatgga acggcaagaa
     5521 aaaaggatct taccggcgcg gtcg
//
LOCUS       DATGKE010000091         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_184420, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000091 DATGKE010000000
VERSION     DATGKE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184420"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VKU83_01600"
     CDS             <1..919
                     /locus_tag="VKU83_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLI92267.1"
                     /translation="EQCIGAKVNHKLVPLSHKLRSGDQVEIITSSKQKPNEDWLGIVV
                     TAKAKSKIKDALKDEKRKIADEGKYIVQRRLENFGAGYNQHNIDVLTTFYKLSSSLDF
                     FYQVAVHSLDLKELKEFQVLGDRLEPPKPPKPAQENRTGETVPPSVPRKDTELIIFGE
                     SSDRIVYTLANCCKPIPGDDVFGFVSVGKGLTIHRTNCPNAAKLMANYGHRVVKTKWA
                     KNKEISFLTGVRIVGMDDVGVINKITNLISGELKLNIAALTIEAKEGLFEGNIKIYVH
                     DKEELEELVSRLMQLNGIHTVVRYDTETT"
     gene            969..2240
                     /locus_tag="VKU83_01605"
     CDS             969..2240
                     /locus_tag="VKU83_01605"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLI92268.1"
                     /translation="MVDVILGLQWGDEGKGKIVDFFAPRYDVIARFQGGPNAGHTLYV
                     QDKKIVLHQIPSGIFHERTVNLIGNGVVLDPVTLRRECESVSAFGVDYRKNLYIAQRT
                     HLILPTHRALDKASEMSKGQEKIGSTLKGIGPAYMDKTGRNGLRVGDLLDKSFTTQYI
                     RLRLKHQKLLDSLNFHEDLSAWEEEFFEALDFMRQFKIVNGEYFINDKIAQGKTVLAE
                     GAQGSMLDVDFGTFPFVTSSNTISAGVCTGLGVPPQQIRDVIGVSKAYCTRVGSGPFP
                     TELEDATGEELRKTGNEYGATTGRPRRCGWIDGVALKFACMINGVTRIVMTKADVLDA
                     FAELNICTGYKINGETTHEIPFQMNKVKIEPVLQRFDGWRSDITGVKKYEDLPVKMRE
                     YVQYINQYLGVSIAYISNGPGRDQLIENVGR"
BASE COUNT          644 a          648 c          627 g          500 t
ORIGIN      
        1 ggaacaatgt atcggcgcca aggtcaacca taagctcgtg ccccttagcc ataaattgcg
       61 cagcggcgac caggtcgaga tcatcacctc cagcaagcaa aaacccaatg aggattggct
      121 gggcatcgtc gtcaccgcca aagcaaaaag caagatcaaa gacgcgctga aagacgagaa
      181 aagaaagatc gccgacgaag gtaaatacat cgtccagcgc cggctcgaaa atttcggggc
      241 aggctataac cagcataaca tcgacgtcct tactactttc tataaattgt cctcgtcgct
      301 ggacttcttt tatcaggtcg cggtccatag cctcgatctg aaagagctga aggagttcca
      361 ggtgctgggc gaccggctgg agccgcccaa gccgccgaag cccgcccagg agaaccggac
      421 gggcgagacc gttcccccgt cagtcccgcg caaggatacc gaactcatta ttttcggtga
      481 gagcagtgac cgtatcgtct atacgctcgc caattgctgc aaacccattc ccggggacga
      541 cgtctttggg tttgtttccg ttggcaaggg cttgaccatt catcgcacca actgtcccaa
      601 tgccgccaag ctcatggcca attacggaca ccgcgtcgtc aagaccaaat gggctaagaa
      661 caaggagata tccttcctca ccggtgtcag gatcgtaggc atggacgacg tcggtgtcat
      721 taacaaaatt accaacctga tctccgggga gctcaagctg aatatcgccg cgctgacgat
      781 cgaggcgaaa gaagggcttt tcgagggaaa tatcaagatc tatgttcacg ataaggaaga
      841 actggaagag cttgtatcca ggctcatgca gctcaacggc atccataccg tcgtccgtta
      901 cgatacggaa acaacataaa atatcgttga cttcacctaa ctttgcccgc ttcaaataaa
      961 gtaattacat ggttgatgtc atcttaggcc tccagtgggg cgatgaagga aaaggaaaga
     1021 tcgtggattt tttcgcaccg cgatatgacg tgatcgcccg tttccagggc ggtcccaatg
     1081 ccgggcatac gctctacgta caggataaga agatcgtgct ccaccagata cccagcggca
     1141 tctttcacga gagaacggtg aacctcatcg ggaacggcgt agttctcgac cccgtgaccc
     1201 tgcgccggga atgcgagtcc gtgtctgctt tcggcgtcga ttaccggaag aacctctata
     1261 tcgcccagcg tacccatctc attctcccta ctcaccgcgc cctcgacaag gcatcagaaa
     1321 tgtcaaaagg acaggaaaag atcggcagca cgctcaaggg catcggcccg gcctatatgg
     1381 ataaaacggg ccggaacggc cttagggtgg gagatctgct ggacaaaagc tttaccaccc
     1441 aatacatcag actccggcta aagcaccaga agctgctcga tagcctcaat ttccacgagg
     1501 acctctcggc ctgggaagaa gagttcttcg aagcgctcga tttcatgcgg cagttcaaga
     1561 tcgtcaacgg cgaatatttt atcaatgata agatcgcgca gggcaagacg gtgctggcag
     1621 agggtgcgca gggaagcatg ctcgatgtcg atttcgggac cttcccattt gtcacctcct
     1681 ctaatacgat ctctgccggc gtttgcacgg gtctcggtgt cccgccccag cagatccgcg
     1741 acgtcatcgg cgtcagcaaa gcctactgta cccgggtcgg cagcggcccc ttcccaaccg
     1801 aactggaaga cgctacggga gaggagctcc gcaagacagg gaacgaatac ggggcgacta
     1861 ccggccgtcc ccgccggtgc ggatggattg acggtgtggc acttaagttc gcctgtatga
     1921 tcaatggcgt caccaggatc gtaatgacca aggccgatgt gctggatgcc tttgccgagc
     1981 ttaacatctg taccggctac aagatcaacg gcgaaactac ccacgagata cccttccaga
     2041 tgaataaggt aaagatcgag ccggtcctgc agcgattcga cggctggaga tcggatatca
     2101 ccggcgtcaa gaagtatgag gacttaccgg taaaaatgcg ggaatacgtg cagtacatca
     2161 accagtacct gggagtgagc attgcttata tttccaatgg ccccggcagg gatcaactca
     2221 ttgaaaatgt aggtcgttaa aattcacggc gggaatcatc aacaaaaaaa tttccttgcg
     2281 tgaaaacagc taattttgga ccttcggtca aggcggctgc gctgtctttt gtgtcgaaaa
     2341 aattatctaa aataaatttg gcggattaaa aacttcgccg aaaatttaca taacaatctt
     2401 attggacatg gcaccaaaa
//
LOCUS       DATGKE010000092         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1324309, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000092 DATGKE010000000
VERSION     DATGKE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324309"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VKU83_01610"
     CDS             complement(<1..363)
                     /locus_tag="VKU83_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92269.1"
                     /translation="MDNSNKPVRALWFANTPGLSAGHLGLSFSGGGWISSLQREVEAE
                     QACQLGFVFYAEKAMAPFEYGRTWYFPVQPLGSNKRKRLVNRLTRRAESDENLAAWLR
                     AIDEFKPDIIHVHGTEFSF"
     gene            complement(482..2809)
                     /locus_tag="VKU83_01615"
     CDS             complement(482..2809)
                     /locus_tag="VKU83_01615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLI92270.1"
                     /translation="MIRLVTIFFCGLSLPGFSQVFSKEDRGRCEAEAKEVKIVRDKWG
                     IPHIYGKTDADVVFGLLYAECQEDFPRVEKNYLEQLGRQAEAFGDSYLYTDIMMRLIY
                     DSAAAVADYNNCPAWMHKLLTAFADGVNYYLYKHPETKPLVLKHFEPWYPLMFTDGSV
                     SATSLGGVRMDEIKSFYGAPQAGAGAPGAGAVVPGKHRPVSAAEKMVLTDRGIISPGI
                     GVSGDAAADGGAADAPAETETPDGGSNGFALSGFRTASGSTELYINPHVPFYFRYEVQ
                     LVSDEGLNAYGAVTWGQFFVYQGFNAHCGWMHTSSLADVADLYAEKVRREGDGWVYEY
                     DGQARPVKSKPFTIYVSRDDSLIAFPVTGLYTHHGPVMASRNGKWLSLREYNRSLNAL
                     LEAWLITKANSFDEYKKAMDLRANTTNNTVYADDQGNIAYWHGNFMPRRDTSFDWSLP
                     VDGSIAATEWKGVHELDEIVHSYNPATGWIQNCNSTPWSLAGGASPVRDKYPKYMAPD
                     GENFRALLAVKLLAGANDFTLDSLIAKGYDHYLMAFDKLLPALFAAYNTAPDSTRTAL
                     QEPIAILQKWDRRSAARSVATTLAVDWGGRVMRYAQRPATAEAASEAVAMVESAVANS
                     TAGEKMEALRIVLQELRQRYGTWKVEWGDICRYQRLTGKILETYDDRKPSLAVGLVSS
                     AFGQIPSYVSRVMPGTNRRYGVSGNSFIAAVEFGKRIKARTIVTGGESSNPASPHFTD
                     QAAMYLTGRFKDVLFYPDEVQAAAEQTYHPGEEQR"
     gene            2871..3695
                     /gene="fdhD"
                     /locus_tag="VKU83_01620"
     CDS             2871..3695
                     /gene="fdhD"
                     /locus_tag="VKU83_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLI92271.1"
                     /translation="MPAVTYHPVQKITADRHHNGNSLDAIAIEEPLEISLQFTRAGAP
                     VRQTIAVTMRTPGHDADLACGFLFTEGIVSDPGAIAAITVADANHAIVTLKEDCHPHL
                     VNASRHSYMNSSCGVCGKTSIDSIFQQVQPTGDSLSLDAGLLYSLPAALTRKQDIFTA
                     TGGLHAAALFECDGEMIAVREDIGRHNALDKTIGFALSQRRLPLSNSILLLSGRACFE
                     LIQKAAMAGIKVVAAIGPPSSLAISLAEKPDITLVGFLREQRFNIYTGARRITFDS"
BASE COUNT          791 a         1174 c          913 g          833 t
ORIGIN      
        1 aaaggaaaat tccgttccgt gcacatgaat gatgtccggc ttgaactcgt cgatcgcccg
       61 cagccaggcc gccaggtttt catcgctttc cgcccgcctc gtcaacctgt tgaccagcct
      121 tttccgtttg ttgctaccca atggctggac gggaaagtac caggtccgcc catactcgaa
      181 aggcgccatc gctttctctg cataaaatac aaacccaagc tgacatgcct gctccgcctc
      241 gacttcccgt tgcaatgaag aaatccaccc gccgcccgag aaactgagtc ccaaatgtcc
      301 agcgctcaaa cccggcgtat tggcgaacca caacgcccgc accggcttat tgctattatc
      361 cattttatgc ttgttactgg tcccgcaagg taatattttt aggcgggcag acggacagat
      421 gaaaaaactg taccagtttt agcgggcggc tcattttcgt tttcgaaata caatgttccg
      481 gtcatctctg ttcctcgccc gggtgatagg tctgttcggc ggccgcctgg acctcgtccg
      541 ggtagaacag cacatctttg aaccttccgg taagatacat cgcggcctga tcggtaaaat
      601 gcggggaagc ggggttgctg ctctcgccgc ctgtgacgat ggttcgcgcc ttgatccttt
      661 ttccgaactc tacagcggct ataaaactat tccccgacac cccgtaccgc ctgttcgttc
      721 cgggcatcac ccggcttaca tacgagggta tctgcccgaa agctgacgac acgaggccaa
      781 cagccaggct gggcttccgg tcgtcatagg tctccaggat cttgccagtc agccgctgat
      841 acctgcagat atccccccac tctaccttcc atgttccgta ccgctgccgc aattcctgca
      901 acacgatccg cagcgcctcc atcttctccc cggccgtact gttggccact gccgactcca
      961 ccatcgccac cgcctccgac gcggcttcgg ccgtagcagg ccgttgcgca tacctcatga
     1021 cccggccacc ccagtccacg gccaaggttg ttgccaccga ccgggccgcc gagcggcggt
     1081 cccatttctg aaggatggcg ataggctcct gaagggccgt ccgggttgaa tcgggcgcgg
     1141 tgttatacgc tgcaaacaaa gccggtaata gtttgtcgaa tgccatcagg taatgatcat
     1201 aacccttcgc tataaggctg tccagcgtaa agtcattcgc tcccgccaac aacttaacgg
     1261 caagcagggc ccgaaagttt tcaccgtcgg gcgccatgta tttcggatat ttatcccgta
     1321 cagggctcgc tccgccggcg agcgaccagg gcgtcgagtt gcaattctgg atccaaccgg
     1381 tcgccggatt atacgagtgc acaatctcgt ccagttcgtg cacgcccttc cactccgttg
     1441 ccgcgatgct gccgtctacc ggcagggacc aatcgaagga agtatcgcgc cgcggcataa
     1501 aattgccatg ccagtaggcg atattgccct ggtcatccgc atataccgtg ttgttcgtcg
     1561 tgttggctct gaggtccatg gccttcttgt attcgtcgaa gctgttcgcc ttggtgatga
     1621 gccatgcctc cagcagggcg ttcaatgacc ggttgtattc ccggaggctg agccatttcc
     1681 cgttacggct cgccatgacg ggaccatgat gggtgtataa accggtcacc gggaaggcga
     1741 tcaacgaatc gtccctcgaa acatagatcg taaaaggctt gctcttcacc ggccgcgcct
     1801 ggccatcata ttcatagacc catccatcgc cctcccgccg cactttttcg gcgtacaggt
     1861 cggccacatc cgcaagactg gacgtatgca tccatccaca atgcgcgttg aaaccctgat
     1921 acacaaagaa ctggccccag gtgacagccc catatgcatt cagcccttca tcgctgacca
     1981 actgcacctc gtagcggaaa taaaaaggca catgcggatt gatatacaat tcagtactgc
     2041 ccgaggccgt ccgaaatccc gataaggcaa agccgttgct ccccccgtcc ggcgtttccg
     2101 tctcagctgg cgcgtcagca gcgccaccat cggcggctgc gtctcccgaa acaccaatac
     2161 ccggcgaaat gatccccctg tcggtcaaca ccattttttc ggcggccgat accggccggt
     2221 gtttccccgg gactaccgct ccggctccgg gagcaccggc gcctgcctgc ggcgcgccgt
     2281 aaaagctttt aatttcatcc attctgacac cgccgaggct cgtcgctgaa acgcttccat
     2341 ccgtgaacat caggggatac cagggttcaa aatgcttcag taccagcggc tttgtttccg
     2401 gatgcttata gaggtaataa ttaactccgt cggcaaacgc agtcaaaagt ttatgcatcc
     2461 atgcggggca gttattataa tcggctacgg ccgccgcgct gtcatagatc agccgcatca
     2521 tgatgtccgt atacagatag ctgtcgccaa atgcctccgc ctgtcgtccc aactgttcca
     2581 ggtaattctt ctcgacccgt ggaaagtcct cctggcattc ggcgtaaagt aatccgaaaa
     2641 ccacgtcggc atctgttttc ccgtagatat gcggaattcc ccacttgtcc cggacaattt
     2701 tcacctcttt ggcttcggct tcacagcgcc cgcggtcttc tttcgaaaaa acctgggaga
     2761 atccgggcaa cgataaaccg caaaaaaaga tcgttacaag tctaatcatg ccctaattta
     2821 gtgttttata gccgttccag ccgcctgcat tttgcgtaaa tttgaccatc atgccagcag
     2881 tgacttatca cccggtccaa aaaataactg ccgaccgcca tcataacggc aacagcctcg
     2941 acgcgatcgc tatcgaagaa ccattggaga tcagccttca gttcacccgt gccggcgccc
     3001 ccgtccgtca gaccattgcc gtcaccatgc ggacccccgg tcacgatgcc gaccttgcct
     3061 gcggtttcct ttttacggaa ggtatcgtgt cggatccggg cgctatcgcg gcgatcaccg
     3121 tcgcggatgc caatcatgcc atcgttaccc tgaaagaaga ttgccaccct catctcgtaa
     3181 acgcatcgcg ccattcctat atgaattcca gctgcggagt ttgcggaaag acttcgatcg
     3241 attcgatctt ccaacaggta cagccaaccg gcgacagcct tagcctggac gcggggctac
     3301 tctattcgct gcctgccgcg ctcaccagga agcaggacat tttcaccgcc acaggaggcc
     3361 tccatgccgc cgcccttttc gaatgcgacg gcgaaatgat cgcggtccgc gaggatattg
     3421 gccgccacaa cgccctggat aaaaccattg gcttcgcact ttcgcagcgg cggctgccgc
     3481 tttcaaattc cattctgctg ctgagcggcc gggcatgttt tgagctcatt caaaaagccg
     3541 cgatggccgg cataaaagta gttgcagcca tcgggccgcc ttccagcctg gcgatcagcc
     3601 tcgcagagaa accggacatt accctggtgg gtttcctgcg tgaacaacgt ttcaatattt
     3661 ataccggcgc ccggcggata acgttcgact catgaagcac cttcataccc g
//
LOCUS       DATGKE010000093         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_597468, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000093 DATGKE010000000
VERSION     DATGKE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597468"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VKU83_01625"
     CDS             complement(<1..1603)
                     /locus_tag="VKU83_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92272.1"
                     /translation="MSPVIRSLSRASFVVSLLCLRFAGFSQADSYPYKVAQDRMYWHD
                     KVDQAQERIVIMGDGKADSVIRLTRDSAVNLQVTDAICRRVDKLQEEIEFDSTLNTNA
                     KKRYLRGLEDMLNGFGKAFQSGQVPASMAPRLVSAFAQAMRLDERSLSIEPVIAAAPY
                     PVGNILVECFLYPTPNAGVAPSRLLLTRRYCERHPGLIFSYLSSHPGLPFEDSLIVVA
                     GHYDVGQLYNFAAAGNQLAWRIRNSKDSLVHTVAIIANSRSGQLYLPFLDQLVRGRIT
                     TDDIDRVKNDDLNYFRLLVRTRLDYAARVLPPQRDTPLEMNALTSMLEKKCKQVFVNE
                     INALHTVENPAVRFRILDPLTPEELYYIIVMSEDEIYTSSYLGVYDRIFQRMRHPYGD
                     SLLMEVHGDYFRKFIKMAAAYNKLEHFLGTMDKQNAGTVMKSFVIHLENAGEEEAVDV
                     ADSYSSIVEKSPALARFILGEVKWNYQKNVAEANSKGIIIYHLLQTLFESADTANHID
                     LSSALGIPPVYTVDHGSLVDDSGRVV"
     gene            1847..2527
                     /locus_tag="VKU83_01630"
     CDS             1847..2527
                     /locus_tag="VKU83_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92273.1"
                     /translation="MDSKARKILIADDEPDILEIIQYNLTKEGYEVSTAKDGDEAIAK
                     AKTVRPDLIILDIMMPKKNGVEVCEILRAQPPFKETLILFLTALSDEGSHVRGLETGA
                     DDYVTKPISPKLLTSRVNALFRRVSKEPEDKVLNVENLSIDPAKFEVTVDGTDVTLAK
                     KEFELLYLLASRPGRVFLRNEILNQVWGNEVIVGDRTIDVHIRKIRQKLGLDCITTVK
                     GVGYKFNM"
     gene            2576..3625
                     /locus_tag="VKU83_01635"
     CDS             2576..3625
                     /locus_tag="VKU83_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92274.1"
                     /translation="MPSTKNLSPRQLSALTAILLAVPISAGIDLLTGNPLIGAASLVI
                     VFGGCYGLILFTLEKFIYRKIKLIYKLIHQTKATKKEEVYFKYILPQKSIDEVREDVE
                     KWGEKRNEEIELLKKNEAYRKEFLQNLAHEFKTPIFAIQGYVDTLLNGAMDDTMIRKK
                     FLENTAKNVERLVNLMNDLDEISTLERGEQLLYKQNFIIQDLIREIFESLSIKTAGKN
                     IRTSIKKGCESPVTVFADKEKIRMVLINLVENATKYGKNGGGIVASIYKIDEKNVLVE
                     ISDDGIGIEEEHLLRIFERFYRTDAARSRDKGGTGLGLAICKHIIEAHGQSVHVRSRP
                     DVGTTIGFTLESRRD"
     gene            3678..>3874
                     /locus_tag="VKU83_01640"
     CDS             3678..>3874
                     /locus_tag="VKU83_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92275.1"
                     /translation="MTTTTPIFDYGKWTTQLAELSKRYQSAKPYPHIVLENFLDPAVL
                     RHCVAEFNHLNETDGWINYKH"
BASE COUNT          946 a         1052 c         1066 g          810 t
ORIGIN      
        1 cgaccaccct gccgctgtca tcgaccaggg aaccgtggtc cacggtatag accggcggga
       61 tacccagtgc gctggacagg tcgatatgat tggccgtgtc cgcggattcg aaaagggtct
      121 gcagcaggtg atagatgatg atgcccttgc tgttggcttc tgccacattc ttttgatagt
      181 tccattttac ctctccgagg atgaacctcg caagggccgg gcttttctca acgatgctgc
      241 tataagaatc ggcgacgtcc acggcctctt cctcaccggc gttctcgaga tggattacga
      301 aggatttcat gaccgtgccg gcgttttgct tgtccatcgt acccaggaaa tgctcgagtt
      361 tgttgtaagc tgcagccatc ttgatgaact tgcgaaaata atccccgtgc acctccatca
      421 ggaggctgtc gccataagga tgccgcattc gctgaaagat gcggtcatag acgccgagat
      481 aactgctggt atagatctcg tcctcgctca tcacaatgat atagtagagc tcttccggcg
      541 taaggggatc gaggatcctg aagcggacgg ccgggttctc caccgtatga agggcgttga
      601 tctcgttgac gaagacctgt ttgcacttct tctccagcat ggaagtgagg gcattcattt
      661 ccagcggcgt gtcgcgctgc ggcggcagca cccttgccgc ataatcgagg cgggtcctga
      721 ccagcagccg gaaatagttc aggtcgtcgt tcttcacccg gtcgatgtca tccgtggtga
      781 tcctgccccg caccagctgg tcgagaaaag gcagatacag ctggccgctc cggctgttcg
      841 cgatgatcgc taccgtatgg accagggaat ccttactgtt gcggatccgc caggccagct
      901 ggttgccggc ggcagcaaaa ttatacagct ggccgacgtc atagtggccc gcgacgacga
      961 tcaggctgtc ttcgaaagga agtccggggt ggctggataa atagctgaag atcaggcctg
     1021 gatgcctttc gcagtagcgt cgcgtcagga ggagccgcga cggcgctacg ccggcattgg
     1081 gagtgggata aaggaaacat tcgaccagga tatttccgac cggataaggc gcggccgcaa
     1141 tgaccggttc aatgctcagc gatcgctcgt cgagccgcat ggcctgggca aaagccgata
     1201 caagtcgggg agccatcgac gccgggacct gaccggactg aaaggccttt ccgaatccgt
     1261 tcagcatgtc ttccagtccg cgcagataac gtttttttgc gttggtgttc aacgtggagt
     1321 caaattcgat ctcttcctgg agtttgtcga cccggcggca gattgcgtcg gtgacctgca
     1381 gattgacggc gctgtccctg gtcaggcgaa tgacgctatc ggcttttccg tcgcccatga
     1441 tcacgatccg ctcctgggcc tggtctacct tgtcgtgcca gtacatccgg tcctgggcca
     1501 ccttgtacgg ataggaatcc gcctgggaaa atcccgcgaa acggaggcaa agcaacgaca
     1561 ccacaaaaga agccctcgat agggatcgta taactggact cataagatat ctcagtgaat
     1621 aatgtgccgg aaaggcactg attcaaaagt actgaaaaac tacgcatttg caggagccaa
     1681 atgctgggaa cggccttctt cccgcgccgg cgggcgacgg ggcttcgccg gaatgggcca
     1741 tccttccgct cgccccttgt tatttatgta ttaataaatt gtaaacagat cccgcaaatt
     1801 tccggcccaa tctttatttc cagtaggttt gtgtaaactt ttgtgaatgg actccaaggc
     1861 gcggaagata ctgatcgctg acgacgaacc cgatattctg gaaatcatcc aatacaacct
     1921 cacaaaggag ggatacgagg taagcacggc caaggatggc gacgaggcca tcgccaaagc
     1981 caaaaccgtc aggccggatc tgatcatcct cgatatcatg atgcccaaaa agaacggtgt
     2041 cgaggtttgc gagatcctgc gggctcagcc acccttcaag gaaaccctga tcctgttcct
     2101 gaccgccctg agcgacgaag ggtcccacgt ccgcgggctg gagaccggcg cggacgacta
     2161 tgttacgaag ccgatcagcc ccaaattgct caccagccgg gtgaatgcgc ttttccgccg
     2221 ggtttccaag gagccggagg acaaggtact gaacgtcgag aacctgagca tcgacccggc
     2281 gaaattcgaa gtgacggtcg acggaaccga tgtcacgctg gccaaaaaag agttcgagct
     2341 gctatacctg ctggcctccc ggccaggcag ggttttttta aggaacgaga tcctgaacca
     2401 ggtgtggggc aacgaagtga tcgtcggcga ccggacgatc gacgtgcaca ttcgcaagat
     2461 ccgccagaaa ttggggctgg attgcatcac aaccgttaag ggagtaggct ataagttcaa
     2521 tatgtaggac ggccgcaatc tccatgaggc ttgtatcttt gtcattattg ctcccatgcc
     2581 ttccaccaaa aatctttcgc cccggcagtt atccgcgcta acggccatcc tgcttgccgt
     2641 cccgatctcg gcggggatcg atttgctcac cgggaacccg ctgatcggcg ccgcctcgct
     2701 ggtgatcgtt ttcggcggct gctatgggct catcctgttc accctggaga aattcattta
     2761 ccggaagatc aagctgatct acaaactcat ccaccaaacc aaggcgacaa aaaaagaaga
     2821 ggtctacttc aaatatatcc ttccccaaaa gagcattgac gaggtgcggg aagacgtgga
     2881 gaaatggggt gagaaacgaa acgaggagat cgagctttta aaaaagaatg aagcgtaccg
     2941 caaagagttc cttcaaaacc tcgcgcacga gttcaaaaca cccattttcg ccatccaggg
     3001 ttatgtggac acgctattga acggggcgat ggatgacacg atgatccgga aaaaattcct
     3061 ggagaacacg gcaaagaacg tcgagcggct cgtcaatctg atgaacgacc tcgatgagat
     3121 ctccacgctc gaaaggggcg aacagctgct ctataagcag aatttcatta tccaggacct
     3181 tatcagggaa atcttcgagt ccctctccat caagacggcc ggcaagaata tccggacctc
     3241 gataaaaaaa ggctgcgaat cgccggttac cgtctttgcc gacaaggaaa agatccggat
     3301 ggtgctgatc aacctggtcg aaaacgcgac caaatacggc aagaacggcg gcgggatcgt
     3361 agccagcatt tacaagatcg acgagaagaa cgtgctggtc gagatcagcg acgacgggat
     3421 cggaattgaa gaagaacatc tgctgcggat attcgaacgg ttttaccgga cggatgcggc
     3481 cagaagccgc gataagggcg gcaccgggct tgggctggcc atctgcaaac atattatcga
     3541 agcccatggt cagtccgtcc atgtccgaag ccggcccgac gtcggcacca cgatcgggtt
     3601 caccctggaa tccaggcggg actgaccttc ctgacccctg gctcggttaa cggaaaatct
     3661 cactactttt gcctccgatg acaacaacca cacccatctt cgactatggc aaatggacga
     3721 cccagctggc agaactcagc aagaggtatc agtcggcaaa gccttatccc cacatcgtgc
     3781 tggaaaactt cctcgatccg gctgtactca ggcattgcgt cgcagaattc aaccacctga
     3841 acgaaacgga cggatggatc aattataagc atta
//
LOCUS       DATGKE010000094         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1117786, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000094 DATGKE010000000
VERSION     DATGKE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117786"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VKU83_01645"
     CDS             <1..945
                     /locus_tag="VKU83_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92276.1"
                     /translation="EEIERQTTRNGKMTEPDAVAGDENVLNLHFSETDAEGNPVEAGI
                     EKDNSLLVKYFNEATRPKWIGLKKGDSILLQPSAAFDEKEREWVLGDLGLSKENPADA
                     GRYFRTTITKVGLIEKAALDENFFKAVYPGRDITTLEAFRDAVKDEIQAYWDNQSRNQ
                     LQHSLYHVLLDQTRIEFPEEFLKRWMQTGGDKPKTQTEVDQEFPAFADQLKWTLILDK
                     IVAENGIEVSQDDIKAFARQQLMGYMGHQAGGEDQPWIADYVQRMMQDRKFIEDTVHR
                     IRTEKVFAWAETRIHPTEKPVSRDEFRNMVEGHQHHHH"
     gene            1102..1800
                     /locus_tag="VKU83_01650"
     CDS             1102..1800
                     /locus_tag="VKU83_01650"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLI92277.1"
                     /translation="MNPANEFEKYAVKHRGISSNTLNDYNKYLVTNLTPNIIEERPMN
                     VAVMDVYSRLMMDRIIFLGYPINEEVANIITAQLLFLDSTDRHRDIQMYINCPGGSVY
                     SGLGVYDTMQYVTPDVATICIGMAASMGQVLMCAGTKGKRTALRHSRVMMHQPSGSIG
                     GQASDIEITVSEIRKLRKELYEITAFHTGKTVKQVEKDSDRDYWLTAQEAKDYGLVDE
                     VLLQNPRKERDGNH"
     gene            1823..2464
                     /locus_tag="VKU83_01655"
     CDS             1823..2464
                     /locus_tag="VKU83_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLI92278.1"
                     /translation="MNYSKYLPDSIWRNDDEPDEKEPKEEKGPDLGMLAKKMEKYFFE
                     KRTVELWGPVDDKSAREVVTKLRLLDADKPGEEIKFFINSPGGVVTSGMVIYDTMKML
                     KSPVSTICMGLAASMGSLLLSGGAKGRRFVFPHSEIMIHQPSLGGYMQGVSVDLEITA
                     KQTRRVKEVSAKILADNCGKKVEQILKDFDRDYWMDAKEAVEYGIVDQIFEQI"
     gene            2547..3782
                     /gene="clpX"
                     /locus_tag="VKU83_01660"
     CDS             2547..3782
                     /gene="clpX"
                     /locus_tag="VKU83_01660"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLI92279.1"
                     /translation="MAKNTLHCSFCGRNRDEVKILIAGQEGHICENCVEHAQEIIQQE
                     LMIREDSPGSSTFKLTVKKPIEIKRILDEYVIGQDDAKKVLSVAVYNHYKRLQQKVVE
                     NEVEIEKSNIIMVGETGTGKTLLAKTIARLLNVPFAIVDATVFTEAGYVGEDVESILT
                     RLLQVCNYDVPAAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGTDVL
                     VPPQGGRKHPEQKLIKVNTQNILFICGGAFDGVDKIIARRINTNAIGFNVNKEQQEAA
                     KKNLLQYVNAPDLKSFGLIPELLGRLPVVTHLDPLDKPTLRSILTEPKNSLIRQYKKL
                     FELEGIQLGIDVDVLDFMVDKAVEYKLGARGLRSICESILTDAMFELPSAKEEKFHLD
                     LEYAQKKFDKSKMNLLKVA"
BASE COUNT         1032 a          937 c         1106 g          711 t
ORIGIN      
        1 gaagagatcg aacgccagac gacccggaat ggcaaaatga cggagcccga cgcggttgcg
       61 ggagatgaaa atgtgctgaa cctgcatttt tccgaaacgg acgcggaagg caacccggtg
      121 gaagcgggga tcgaaaaaga caattccctg ctggtaaaat actttaacga agccacgcgg
      181 cccaaatgga tcggcctgaa gaaaggcgac tcgatcctgc tccagccgtc cgccgccttc
      241 gatgagaagg agcgcgaatg ggtcctgggc gatctggggc tttcgaagga aaaccccgct
      301 gacgccggca ggtatttccg cacgaccatc acaaaggtcg gcctgatcga aaaggctgcg
      361 ttggacgaga acttcttcaa ggccgtatac ccgggcagag acatcacgac gctggaggct
      421 tttcgggatg cggtaaagga cgaaatccag gcctattggg acaatcagag ccgaaaccaa
      481 ctccagcact cgttgtatca cgtattattg gaccagacgc ggatcgaatt tccggaagag
      541 ttcctgaagc ggtggatgca gacaggcggc gacaagccga agacccagac ggaggtcgac
      601 caggaattcc ccgccttcgc cgatcagctt aaatggacgc tgatcctgga caagatcgtt
      661 gccgagaacg gcatcgaggt cagccaggac gacatcaagg cctttgcccg ccagcaactg
      721 atgggctata tgggccacca ggcggggggc gaagaccagc cctggattgc cgactatgtc
      781 cagcggatga tgcaggatcg aaagttcatc gaggatacgg ttcaccggat ccgcacggaa
      841 aaggtttttg catgggcgga gacaaggatc catccaacag agaagccggt cagccgcgac
      901 gagttccgca atatggtgga aggccatcag catcatcatc attagatttg tcgacgtccc
      961 cggaaccatc tccgggcgca accattcggc gcttgtgccg tattgtcagt tttttggcag
     1021 tttgaaagtt tggacggaga tttgaaagga agttctcgtc cataggatag aattggcgtt
     1081 aaaacattga aaaatatcat catgaatcca gcaaacgagt ttgagaagta tgcggtaaag
     1141 caccggggca tctccagcaa cacgttgaac gactacaata aatatcttgt tacgaacctc
     1201 acgccgaata tcattgagga gcggccgatg aatgtcgccg ttatggacgt ctacagccgg
     1261 ctgatgatgg accggatcat cttcctcggc tatccgataa acgaggaagt ggcgaacatc
     1321 atcaccgctc agctgctgtt cctggacagc acggaccggc accgcgacat ccagatgtat
     1381 atcaattgcc cagggggtag cgtttactcc ggcctggggg tatatgacac catgcaatac
     1441 gtgacgccgg acgtcgccac gatctgtatt gggatggcgg ccagcatggg gcaggtgctg
     1501 atgtgcgccg gaacaaaggg caagcggacg gcgttaaggc acagccgcgt catgatgcac
     1561 cagccaagcg gctcaatcgg cggccaggct tccgatattg agatcacggt cagcgagatc
     1621 aggaaactgc ggaaagagct gtatgaaatc accgcgttcc ataccggtaa gacggtgaaa
     1681 caggtggaaa aggacagtga ccgcgactat tggctgaccg cccaggaagc aaaggactat
     1741 ggcctggtcg acgaagtgct gctgcagaac ccgcggaaag aaagggatgg caatcactaa
     1801 cccacacgat aaaaagatta ctatgaacta ttcgaaatac ctgccggatt caatctggcg
     1861 aaatgacgac gaacccgatg agaaggaacc gaaagaggag aagggtcccg acctgggcat
     1921 gctggcgaaa aaaatggaaa aatacttttt cgagaagcga acggtggagc tgtggggacc
     1981 ggtcgatgat aagagcgcaa gggaagtagt caccaagctg aggcttctgg acgcggacaa
     2041 accgggcgag gagatcaagt tcttcattaa tagcccgggc ggcgtggtga cctcaggaat
     2101 ggttatatat gacacgatga aaatgttgaa gagcccggtc agcacgatct gtatgggtct
     2161 cgccgcatcc atgggctcgt tattgctcag cggcggcgcg aaaggccggc ggttcgtatt
     2221 tccgcattcg gagatcatga tccatcagcc cagcctcggc ggttatatgc agggcgtgag
     2281 cgtcgatctc gagattacag cgaagcagac gcggcgggtc aaggaggtga gtgcaaagat
     2341 cctggcagac aattgcggga aaaaggtcga acagatcctc aaggatttcg accgtgacta
     2401 ctggatggat gcaaaagaag ccgtcgaata tggcattgtg gatcaaatct ttgagcagat
     2461 ttagcgtacc acgttgatta ttaatggcaa caaactttga ttgtccacac cctgggagaa
     2521 tcgaataagt tgggtaactt tacacaatgg caaagaacac cttgcattgc tctttctgcg
     2581 ggcgcaaccg ggacgaagtg aaaattttga tcgccggcca ggaagggcat atctgcgaga
     2641 attgcgtcga gcatgcgcag gagatcatcc agcaggagct gatgatcagg gaggattcgc
     2701 cgggaagcag caccttcaaa cttaccgtca agaaaccaat cgagataaaa cggatcctgg
     2761 acgaatatgt gatcggacag gacgatgcga agaaagtatt gtcggtagcg gtatataatc
     2821 attacaaacg gctccagcaa aaggtcgtcg agaacgaggt agaaattgaa aagagcaata
     2881 tcattatggt cggggagacc ggcaccggca agaccctcct ggccaagact attgcaagat
     2941 tgctcaatgt tcccttcgcg atcgtggatg ccacggtctt cacggaagcc ggctacgtcg
     3001 gcgaagacgt cgagagcatc ctgacaaggc tgctgcaggt ctgcaattac gatgtgccgg
     3061 ccgccgaacg cgggatcgtc tatatcgacg agatcgacaa gattgcccgc aaaggggaca
     3121 acccgtccat cacccgggat gtcagcgggg aaggcgtcca gcaggggctg ctcaaactgc
     3181 tggaaggaac ggatgtcctc gtgccgccac aaggaggccg caagcacccg gagcagaaac
     3241 tcatcaaggt caacacccag aatatcctgt tcatctgcgg cggcgcattc gacggcgtcg
     3301 ataagatcat tgcccgcagg atcaatacga acgcgatcgg cttcaatgtc aataaggaac
     3361 agcaggaggc ggcaaagaag aacctgttgc aatacgtcaa tgcgccggac ctgaagtcat
     3421 tcgggctgat ccccgaactg ctcggccggt taccggttgt gacgcacctg gatccgctgg
     3481 acaagccgac gctgcggtcg atccttaccg aaccgaagaa ttcgctgatc cgtcaataca
     3541 agaagctgtt cgaactggaa gggatccaac tcggcatcga tgtggatgtg ctggatttca
     3601 tggtcgacaa ggctgtcgag tacaaacttg gcgcccgcgg cttgcggagc atctgcgaaa
     3661 gcattctgac cgacgcgatg ttcgagctgc cttccgcgaa agaagaaaaa ttccacctcg
     3721 acctggagta cgcccaaaaa aagttcgaca agagtaaaat gaatctgctc aaagtagcgt
     3781 aatctg
//
LOCUS       DATGKE010000095        15336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_806574, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000095 DATGKE010000000
VERSION     DATGKE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806574"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1181
                     /locus_tag="VKU83_01665"
     CDS             18..1181
                     /locus_tag="VKU83_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HLI92280.1"
                     /translation="MDVSYIINELGEEREHYFNAVAPPIIQTSNFRVPKVDQLKALFA
                     DEYSGYLYSRGLNPTVEILRKKLAALDGAEDALVFNSGASAIFAGVLANVKAGDHIVS
                     VDKPYTWAQRMFDVILPRFGVATTYIDGSRIDGFERAILPNTALIYLESPNSWNYKLQ
                     DLAAVAELARAEGIVTMIDNSYCTPLYQRPLDLGIDISMQTATKYIGGHSDTVGGVLC
                     GTRAMIKKIFDSEFLNIGCGIPPFNAWLLLRGLRTLPARLDRISATTRQVLDFLKGHP
                     RVESVLFPLDETFPQYALARRQMKGACGLLSFYVHADSRASIVRFCESLRHIFMAVSW
                     GGHESLILPKCAGLAAEEFDPRNADHRMLRLYTGLEDAAYIIDDLRQAFAAAW"
     gene            1210..2634
                     /locus_tag="VKU83_01670"
     CDS             1210..2634
                     /locus_tag="VKU83_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI92281.1"
                     /translation="MRKPPLFLSLIALLSTLTVRAQDKWDLKRCVDYAVEHNISIKQA
                     DVQARVAKLTLDQSKLMRIPTLSFGSSIDVTAGRSQNPTSFTLNTQTSLYNNLSLQTS
                     LIVFNGFNIQRFIDANRFAWQAMLANSELLKNNISLNVANAYLQVLLADQTAQTAQLQ
                     MQFSKNQLELTRKQVTAGTLPELNAAELESQVAQDSSTYVTAKSNAIQAVFTLKAYMD
                     LDAASPFDVDMPPVDQIPIGQLADLQPDAVYATALTHQPLQKMDALNIQAFQETLRAA
                     KGSMYPTVSLFGSIGTNFINTPGELPVIGTGQIGETQTTHEPVLGQTVTGYYKAPVTP
                     YFTQLNDYLAQSIGLSIQIPIFNQGTLRTNYQKGKLNLRNQELIRDQDNLTLKSNIYQ
                     AYTLAVAALEKFEAQKITLAATQKSYEYAQKRYNVGMSNTIDLLTNQNNYFAAKVNLL
                     YDQFDYVFKMKVLEYYKGMGIKLN"
     gene            2647..4059
                     /locus_tag="VKU83_01675"
     CDS             2647..4059
                     /locus_tag="VKU83_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLI92282.1"
                     /translation="MNKLLLRIIIIVVVIIGLLVLAKSAGWIGKQDGIKVAAEKVARR
                     DITEVVTASGKIYPEKEVKISPDISGEVVELGVSQEGDSVHKGQMLARIYADIYTNQR
                     NQAAAQMDQQEAMVSNLNETLPGLKATMETDKKALDREKQLLDQKVVSQSEYETAQAT
                     YLTAQANYLAALQNVKGNVAGVASARANLDIAAKNLSRTTVVSPIDGVVSLLSIKKGE
                     RVVGNSMMAGTEMMRVADMSRIEAIVDVGENDIPKVQLGDSALVEVDAYNNRKFRGIV
                     TQIASSVTTSAGTATTTVSTNDVTNYKVHIRLSPDSYKDLIDPKRPRHFPFRPGMSAS
                     ADIQTRTHNNVLSVPINAVATREKNTDNPVVGSSQSADDNSGNSDNGNGDNGQNANAK
                     PSSGADLDEVVFVVSADSVKKVKVHTDIQDINYIEIKDGLKEGDEVVTDPYSVISKTL
                     KSGMRIKVVPKDKLFDGNKN"
     gene            4084..5094
                     /locus_tag="VKU83_01680"
     CDS             4084..5094
                     /locus_tag="VKU83_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLI92283.1"
                     /translation="MRFGLIGSGSWATALAKILTDNGNRIAWWVRNPSIIRHIASRHH
                     NPQYLSSVNFDTSLLTLTGAVSEVVAGSDCLVAAVPSAYVVSTLAALDRSAFRGKKIL
                     SAIKGILPEHNLLLNEWLAAEFALDPDDYFTVMGPCHAEEVAAEKLSYLTFSGRQEAS
                     TRELAACFSTEYLNTVINQDVYGVQFAAVLKNIYALGAGIAHGLEYGDNFLSVLIANC
                     ADEMAGFLRKLGVHHIEVGVHRTANYAASVYLGDLLVTCYSLYSRNRTFGNMIGKGYS
                     VKAAQLELNMVAEGYNASKCIYLKNRQVGAEMPIADTIYRILWENVNVAWGMKQIEPY
                     LV"
     gene            complement(5230..6360)
                     /gene="hemW"
                     /locus_tag="VKU83_01685"
     CDS             complement(5230..6360)
                     /gene="hemW"
                     /locus_tag="VKU83_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLI92284.1"
                     /translation="MAGIYLHIPFCRQACVYCNFHFTTSLRNRNDFVTALLKEMERRV
                     DYLGGAVVDTVYFGGGTPSLLYPAELAAILARLKTAFTLSDGAEITLEANPDDMTDRQ
                     KVRDWRSAGINRLSIGIQSFFNEDLKWMNRAHDAAQGEACIRLAQEEGLDNISIDLIY
                     GAPTLADDHWETNVRKAIALAIPHLSCYALTVEPRTALDKMIRLKKSRDISQDDQARQ
                     LLLLMDWTAAAGYEHYEIANFALPGHRSRHNSAYWRGIPYLGLGPSAHSYNGSSREWN
                     VADNARYLAGLAAGDLPPAGKEILTAVQQLNEYIMVSLRTLEGIDPAAVGRRFGSEKA
                     ADLLRRARRYVREGWMTETDRLCLTNEGKLLADGIAADLFFS"
     gene            6391..8178
                     /locus_tag="VKU83_01690"
     CDS             6391..8178
                     /locus_tag="VKU83_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLI92285.1"
                     /translation="MRYIRLLLLIVLIHPLCGRAQYELQIIPVDKDSVFIASQLGLIH
                     SFRSQELCQQYVSGLLPLLRVKGYAAASLDSIGYGPREATLRLYVGAVWRWAAIDTRH
                     ADPTLLAAAAWNDKAFNGKPMDMRIFRYRQQLILDYLENNGYPFAKVGLDSIRLDSGG
                     TLTAALHIDKGPLYRIDSIRVFGNAKISNEFLQRYLNIPNGSIYRKEKLKIISRKILE
                     LPYVQEQQPWSLTMLAEGSVINLYLKPKRSSQVDALVGFLPSSNPVLGNKVVVTGEAT
                     INLQNTLGNGETMRLEWQQLQAGSPRLNLLYQQPFVFSSPFGLTTSFDLYKQDSSYVN
                     INFVVGAQYTFSANQSGSVFLQLASSNLLNVDTVAVIATHALPPQADISSTSLGLTYS
                     YNNTNYRFNPRRGNEMNFLGSAGTKRVHENGQIAGLSDPSDSGFKFASLYDTVKLHSF
                     QFLVKWEGAHYFPIGRAATIKLGVNAGVYSSPNTYRNDLFMIGGYRLLRGFDEQSILA
                     SEYAVGTLEYRYLTGLNSFLFAFADVGYAANHVSDYHLNSLYIGTGAGMAFETKAGIF
                     NMSLAVGRTDTSPFNFHDTKIHLGYVSFF"
     gene            8217..9290
                     /locus_tag="VKU83_01695"
     CDS             8217..9290
                     /locus_tag="VKU83_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HLI92286.1"
                     /translation="MIFTRISIILLTCLLAIGGLQAQTDSAPPPAAQHGRAQDTGSLP
                     AARPLPRRDSARRARPDSLNQLRRGPATGAARDTATSSVKATRSDTLPGNGGVALFPG
                     AGQSPAAMGYWQKVLAENPYLPFTGQPVAETFEVHRSNGKESMFYLIVAILFYFGLIR
                     VFFEKYFNNLMTLFFRVSLRQQQIREQVLQTPLPSLMLNILFVLSAGLYACFLLHYSK
                     FGAGIRFWLLYMDCMALLAAIYLVKFLVLKFIGWVFSISRATDTYIFVVFLVNKMMGM
                     MLLPFLIVIIFSGQQWREVFITISLAMIFVLWAYRILASFRPVRNEIKLTPFYFFLYL
                     CAFEIAPLLLIYKVLLTYLEKVY"
     gene            9336..10109
                     /locus_tag="VKU83_01700"
     CDS             9336..10109
                     /locus_tag="VKU83_01700"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLI92287.1"
                     /translation="MTKNEDKKKILITQPRPETDKSPYFELAKKYDVELAFFPFIRLE
                     PLPAKDFRKQKIDITQFTAVIFTSRNAIDHFFRMCEEMKTSVSQDTKYFCITEAVALY
                     LQKFILYRKRKVFYGADGLNKSMFDVINKHKENEKFLYPCSENQQDNEIVSWLKQNNC
                     GFATPFMYRTISNDVKEILHQSEYDIICFFTPSGVKSLFDNFPKFKQNGTKIGAFGNN
                     TSRAVEEAGLTLDIKAPQPQAPSMVSALEKYLAVTVKKK"
     gene            10169..12232
                     /locus_tag="VKU83_01705"
     CDS             10169..12232
                     /locus_tag="VKU83_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLI92288.1"
                     /translation="MPNRLATETSPYLLQHAHNPVDWYPWGDEALARAARENKPILVS
                     IGYSACHWCHVMERESFEDQETAGIMNDHFVNIKIDREERPDLDHIYMDAVQAITGSG
                     GWPLNVFLTSDGRPFYGGTYFPPRPVHNRPSWKEVLAAVARSYSERRADLEKQAGALT
                     RHLAVAASLGAGNEPAAAVGADGEFTPDLLKQIRDNLLSTADTRDGGFGGAPKFPQTF
                     SIRFLLHYYYFTGDRGARQQACLSLDKMLAGGIFDQLGGGFARYSTDSGWRVPHFEKM
                     LYDNALLVIALCDAYQLTGEPRYREGIEMTLEFADRELSNGRGGYYSALDADSEGEEG
                     KYYVWTRDEIEAVLGADAGVFCARFGVTAEGNWEGKNILVRARDVPSDPGVKARLDAA
                     CRRLLAHRAQRVRPGLDDKILLGWNALMNIACSRAFAALGKEQYRDRARTNMEFLRTN
                     LKGGMIHLYHHSFKDGPKFPAFLDDYAFLIQALMELQEITGDAGYLDEAREITEMVID
                     HFGEEGTPFFYFTHDQQNDLILRKKEIYDGATPSGNSIMAFNLFYLSKLFDVPAWTDR
                     ARQMAGGVKKLVTGHPGSFGTWATLFQALTYTVPEIVITARRPEIARKDFLKEFVPYR
                     VFQSSSQENTHYPLLRDRPAGEVPQFFVCVDRACQLPVNELSALIRLLENVNKIPSKS
                     YNNPI"
     gene            12382..13665
                     /locus_tag="VKU83_01710"
     CDS             12382..13665
                     /locus_tag="VKU83_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLI92289.1"
                     /translation="MKNLSVLTAFSAIFLLASCKLGGKSGKGLLDDGQLHGVAPATAY
                     NLKKPLGMVYIPPGTFHMGPSDEDMNYAFTARNLQISISGFWMDATEITNNEYRQFTN
                     WVRDSIAATLMQYGKDVDGRFAIDWKKAATIKYADKATIEKINQMIVEPDNRIFGRKE
                     IDPNKIVYHSETFDLNAAAQKINEGKPRSQFIEKKDIKVYPDTLVWIRDFSYSYNEPM
                     TKRYYSHPAFGNYPVVGVNWNQASAFCDWRTQYLNAYLEGKHMATESNFRLPTEAEWE
                     YAARGGRSQSMYPWGNYYLRNKRGCLLANFKPGRGNYPEDGGFYTVRADAYWPNDFGL
                     YCMAGNVAEWTSSFYYEGASNFVHDMNPDIRWNAKDSDPPRMKRKVIRGGSWKDVGLY
                     LQTSARTYEYQDTAKSYIGFRCVIDLAPTLSGNRR"
     gene            13794..14567
                     /gene="gldL"
                     /locus_tag="VKU83_01715"
     CDS             13794..14567
                     /gene="gldL"
                     /locus_tag="VKU83_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HLI92290.1"
                     /translation="MSAGVSKSTERLINIVVCVGAAVVIFGALQKILHTELANFFLTA
                     GLLTESLIFLIYAFLPPPGGEMHALVEALPKLAGGTSGNPALDKMDKMLQEADITPAN
                     LGRLSEGFKKFGTTVEKITDVSGALTATTEYTNKTKEAAAALDNMKGAYQNATNTISS
                     FNAAADSTKQFHSQVQVLTKNLGSLNAIYELELQDTNNHLKAMNSFYGNLVKASETMQ
                     GSVTDAKKTQEQIALLAKNLGNLNHVYGNMLSAMQGRQA"
     gene            14696..>15336
                     /locus_tag="VKU83_01720"
     CDS             14696..>15336
                     /locus_tag="VKU83_01720"
                     /inference="COORDINATES: protein motif:HMM:NF023504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92291.1"
                     /translation="MSIPKEPRQKMINMMYLVLTALLALNVSVEVLNAFKTVNTSLEN
                     SNNVVSQKNNLTYTAFQKNLEDPSTKALAEIWSKPAFQVQSYAAAFSTYLEALKTQLQ
                     KESGLKTQDGKAIYNEGDLDAATRMFDQGGHGKKLYDSLANFRNYLLSELDPNNPAYA
                     NANLSELTKQEMITTKAEFAKRLPIDLRIPKSETGEAEKKDPAENWTIRYFHMT"
BASE COUNT         3666 a         4262 c         4130 g         3278 t
ORIGIN      
        1 gccggtaaaa taatgttatg gacgtctcgt atatcatcaa cgaactggga gaagaaaggg
       61 agcattactt taacgcggtg gcgccgccga tcatccagac cagcaacttc agggtaccga
      121 aggtcgatca gctgaaagcc cttttcgcgg acgagtacag cggctacctg tacagccggg
      181 gcctcaatcc gactgtcgag atcctccgga agaagctggc ggcgctggat ggggcggaag
      241 acgccctggt cttcaacagc ggggcgtcgg ccattttcgc gggcgtcctg gcgaatgtaa
      301 aagccggcga tcatatcgtc tcggtggaca agccctatac ctgggcgcag cggatgttcg
      361 acgtcatcct gccccggttc ggcgtggcta cgacgtatat cgacgggagc cggatcgacg
      421 ggttcgaacg ggctatcctg ccgaatactg cgctcattta cctggaatcc cccaactcct
      481 ggaattacaa actgcaggac ctcgccgcgg ttgccgaact cgcgcgggcg gagggcatcg
      541 tgaccatgat cgacaacagc tactgcaccc cgttgtacca gcggccgctc gacctgggca
      601 tcgacatcag catgcaaacc gcaacgaagt atattggcgg ccacagcgat acggttggcg
      661 gcgtcctttg cggtacgcgc gcaatgatca aaaagatctt cgacagtgaa ttcctgaata
      721 ttggctgcgg gattcccccc ttcaatgcct ggcttctcct ccgcggcctg cggaccctgc
      781 cagcccgcct ggacaggatt tcggcgacga cgcgtcaggt cctagacttc ctgaaggggc
      841 accccagggt ggaaagcgtg cttttcccgc tcgatgagac gtttccgcaa tacgcgctcg
      901 cccggcggca aatgaaaggg gcttgcggat tgctcagctt ctatgtccac gcggatagcc
      961 gcgcttccat tgtccggttc tgcgaatcgc tccggcatat cttcatggct gtcagctggg
     1021 gaggccacga gtcactgatc ctgccaaaat gcgccgggct ggcggcggag gagtttgacc
     1081 cccggaacgc cgaccatcgc atgctgcgcc tttatacggg gcttgaggat gccgcttata
     1141 taattgatga tctccggcag gcattcgcag cggcatggta aaaaattatt ataaatttgt
     1201 cacatgagca tgagaaagcc cccccttttc ctatccctga ttgccttact gtcaaccctc
     1261 accgttcgcg cacaggacaa atgggacctg aaacggtgcg tcgattacgc cgtcgagcat
     1321 aatatttcga tcaaacaggc cgacgtccag gcccgggtcg cgaaactcac cctggatcaa
     1381 agcaagctca tgcggatccc gacgctgtcc ttcggcagca gcattgacgt gaccgccggc
     1441 cggagccaga acccgacttc ttttaccctt aatacccaaa cttccctcta taataatctg
     1501 tcgttacaaa ccagcctcat cgtattcaac ggtttcaaca tccaacggtt catcgatgca
     1561 aaccggtttg catggcaggc gatgctcgcc aactccgaat tactaaagaa taatatttcc
     1621 ctgaatgtgg ccaacgccta cctccaggtc ctcctggcgg atcaaacggc acaaaccgcg
     1681 caattgcaga tgcagttttc caagaaccaa ctggagctaa cccggaagca ggttacggcg
     1741 gggaccctgc ccgagttgaa tgccgcggag ctagagtccc aggttgccca ggacagctcc
     1801 acttatgtga cggccaaaag caatgccatc caggctgtct ttacgctcaa ggcttatatg
     1861 gaccttgacg cggcatcgcc tttcgacgtg gatatgccgc ctgtggatca gatccctatc
     1921 gggcaactgg ccgatcttca gccggacgcc gtctatgcta ccgccctgac ccatcaaccg
     1981 cttcaaaaga tggatgccct caatatccag gcgtttcagg agacgctccg ggcggcgaaa
     2041 ggctcgatgt atccgaccgt ctccctgttt ggcagcatcg gcacgaattt cattaatacc
     2101 ccgggcgaac ttccggtcat cggaacggga cagatcggtg aaacgcaaac aacacatgag
     2161 cctgtgctcg ggcagaccgt cacgggctat tacaaggcgc cggttacccc ttatttcacc
     2221 cagctaaacg actacctggc tcaatcgatc gggctttcga tacagatccc catcttcaac
     2281 cagggcacgc tgcggaccaa ttatcaaaaa ggcaagctca accttcgtaa ccaggagctg
     2341 atccgggacc aggacaacct gaccctgaaa tcgaatatct atcaggccta tacgctggct
     2401 gtcgcagcgc tggaaaaatt cgaggctcaa aagatcaccc ttgccgccac ccagaaaagc
     2461 tacgagtatg cgcaaaaaag gtacaacgtc ggtatgagca atacgatcga cctgctcacg
     2521 aatcagaaca attatttcgc cgcgaaagta aatctgcttt atgaccaatt cgactatgtc
     2581 ttcaagatga aagtcctgga atactataaa ggcatgggaa taaaacttaa ttgaatacat
     2641 tgcaacatga ataagttact ccttcggatc atcataatcg ttgttgtcat cattgggttg
     2701 ctggtgctgg cgaaatctgc cgggtggatc ggcaaacagg acgggatcaa ggtcgcggcc
     2761 gaaaaggttg cccggcggga tatcaccgag gttgtgaccg cgagcggaaa gatctaccct
     2821 gaaaaagaag tgaagatcag ccccgatatt tcgggcgagg tggtggagct cggcgtgagc
     2881 caggaaggcg acagcgttca caaaggccag atgctcgcca ggatctatgc ggatatttat
     2941 acgaatcagc gtaaccaggc tgccgcgcag atggaccagc aagaggccat ggtttccaac
     3001 ctgaacgaaa ccctgcccgg gctgaaagcg acgatggaga ccgacaagaa ggcgctggac
     3061 cgggaaaagc aattgctcga ccagaaagtg gtctcgcagt ccgaatatga aacggcacag
     3121 gcaacctatc tgaccgcgca ggcgaattac cttgcggcgc tgcaaaacgt gaaaggcaat
     3181 gtcgccggcg tagccagcgc gcgcgccaac ctcgacattg cagcgaaaaa tctaagccgg
     3241 acgacggtcg tatcaccgat cgacggggtg gtatccctgc tgagcatcaa gaagggtgaa
     3301 cgggtcgtgg gcaactccat gatggccggc acggaaatga tgcgcgtcgc ggacatgagc
     3361 aggatcgagg cgatcgtcga cgtgggggaa aatgacatcc cgaaggtgca gctgggcgac
     3421 tcagcccttg tcgaagtaga cgcctataac aatcgcaagt tccgcggcat cgttacccag
     3481 atcgccagca gtgtgacgac gagcgccggg accgccacga cgacggtgtc taccaacgat
     3541 gtcacgaatt ataaggtgca tatccgcctt tcgccggact cgtataagga tctcatcgat
     3601 ccgaaacggc cgcggcactt tcctttccgg cccgggatga gcgccagcgc cgatatccaa
     3661 acccggacac acaacaatgt gctttctgtc ccgatcaacg cagtggcaac gcgggagaag
     3721 aatacggata accccgtcgt ggggagttcc caatcggcgg atgacaattc cgggaattcg
     3781 gacaacggaa acggcgacaa tggccagaat gccaacgcca aacccagctc gggagcagac
     3841 ctcgatgaag tggtctttgt cgtgtctgca gacagcgtaa aaaaagtcaa ggtccacacc
     3901 gatatccagg atatcaatta catcgaaatc aaggacgggt tgaaggaagg tgacgaagtg
     3961 gtgaccgacc cgtacagcgt catcagcaaa acgctgaaaa gcggcatgcg aatcaaagtc
     4021 gtgcccaaag acaaattgtt cgacgggaat aaaaattgat tccggcatta aaaacggcct
     4081 caaatgcgct ttggactcat tggcagcggc agctgggcta cggcattggc aaagatcctc
     4141 accgataacg ggaacaggat cgcgtggtgg gtccggaatc cttcgatcat ccgccacatc
     4201 gcgagccggc atcacaatcc ccagtatctt tcttccgtca acttcgatac cagcctgttg
     4261 acgctgaccg gcgcggtctc ggaagtggtg gccggttccg attgcctcgt ggcggccgtt
     4321 ccctctgcct atgtggtgtc gacgctcgcc gcccttgacc gctcggcgtt ccgcgggaag
     4381 aagatactct ccgcgataaa agggatactg ccggaacaca acctcctgtt gaatgaatgg
     4441 ctggcggcgg aattcgcgct ggatccggac gattatttta cggtcatggg gccctgccat
     4501 gcggaggaag tggccgccga aaagctttca tacctgacgt tttccggccg gcaggaagca
     4561 tccacacggg agctggcggc ctgtttcagc accgagtatc tgaatacggt catcaatcag
     4621 gatgtatacg gtgtgcagtt cgcggctgtc ctcaagaata tttatgcgct gggagcggga
     4681 atagcgcatg gcctggagta cggggataat ttcctcagcg tgctgatcgc caactgcgcc
     4741 gacgaaatgg ccggattcct ccgcaagctc ggcgtccacc atatcgaagt gggcgtgcac
     4801 cggacggcca actatgcggc gtcggtatac ctcggcgacc tgctggtcac ctgctattcc
     4861 ctatacagcc ggaaccggac cttcggcaac atgatcggga aaggctattc ggtaaaggct
     4921 gcacagctgg aactaaacat ggtggcggaa ggatataatg cgagcaagtg tatctatttg
     4981 aaaaacagac aggtaggagc ggaaatgccg atagctgaca ccatttaccg catcttgtgg
     5041 gaaaatgtta atgtggcatg gggaatgaaa caaatcgagc cgtatcttgt ctaatattta
     5101 aaagcaggct ctatgcccct ctccttccac ggctttggac ccatgaccat ccttgcgatg
     5161 gtgctcatac tctccgcagt gcagggcatc gagtatctgc gggccaggcg ccggcaccgt
     5221 tagcccggtt cagctgaaaa aaaggtccgc cgctatcccg tccgccagca gtttcccttc
     5281 gtttgtcaga cacaaccggt ccgtttcggt catccatcct tcccggacat aacgccgggc
     5341 ccggcgcagc agatccgcgg ccttttcgga accaaaacgc cggccgacag ccgccggatc
     5401 gatgccttcg agcgttctca gcgagaccat gatgtattcg ttcaattgct gaacggcggt
     5461 caggatttcc ttcccggcgg ggggcaggtc gccggccgcg aggcccgcga gataccgggc
     5521 gttatccgcg acattccact ctcgggacga tccgttgtag gaatgggcgg aaggcccgag
     5581 ccccaggtaa gggatgccgc gccagtacgc ggagttatgc cggctccggt gaccgggcag
     5641 cgcaaaattg gcgatctcat aatgttcgta gccggccgcc gccgtccagt ccatcagcaa
     5701 gagcagctgg cgggcctgat cgtcctggct gatatcccgg cttttcttca gccggatcat
     5761 cttgtccagc gcggtgcgcg gctcgacggt cagtgcataa caggatagat gggggatggc
     5821 cagggcgatg gcctttcgga cgttggtttc ccagtggtcg tcggccaggg tgggggcgcc
     5881 atagatcagg tcgatactga tattatccag gccttcctcc tgcgccagcc ggatgcaggc
     5941 ctcaccctgc gcggcatcgt gggcgcggtt catccatttc aggtcctcat tgaaaaagga
     6001 ttggatcccg atactcagac ggttgatgcc cgcggaccgc cagtccctta ccttttgacg
     6061 atccgtcata tcgtcgggat tggcctcgag ggtgatctcc gcgccgtcac tgagggtaaa
     6121 agccgtcttc agcctggcga ggatggcggc cagttcggcc ggatagagca gggaaggcgt
     6181 accgccgccg aaataaaccg tgtccaccac ggcgccccca agataatcga cccgccgttc
     6241 catttccttc aataaagccg tgacaaaatc gttcctattc cggagagaag tcgtaaaatg
     6301 gaagttgcag taaacgcagg cttgtctgca aaaagggata tggagataga tgccggccat
     6361 tcaggtaata aaccacaaaa ttaaccaaac ttgcgctaca tccggctgct gctgctgatc
     6421 gtcctgatcc atccgctttg cgggcgggcg cagtatgagc tgcaaattat cccggtcgac
     6481 aaggattccg tctttatcgc aagccagctt gggctgatcc attctttccg gtcccaggaa
     6541 ctctgtcagc aatatgtgag cggactgctt cccctcctcc gggtcaaagg ctatgctgcg
     6601 gcctctctgg acagcattgg ctacgggccc agggaggcaa cgcttcggtt gtacgtggga
     6661 gcggtttggc gctgggctgc catcgacacc cggcatgccg atccgaccct cctggccgcc
     6721 gcggcctgga acgacaaggc ctttaacggg aagccgatgg atatgcgcat cttccgttac
     6781 cgccagcagc ttatcctcga ttacctggaa aataacggtt atccttttgc aaaagtgggg
     6841 ctcgacagta tccggctgga ctccggcggc acgctgaccg cggcgcttca tattgacaag
     6901 ggcccgctct acaggatcga cagcatccgc gtgttcggga atgccaaaat atccaacgaa
     6961 ttcctgcaac gctacctgaa cattccgaat ggcagcatct accggaaaga aaagctaaag
     7021 atcatcagca ggaagatcct ggaacttccc tatgtccagg agcagcagcc ctggagcctc
     7081 accatgctgg ccgaaggctc ggtcatcaac ctgtatctga agccgaagcg gagcagccag
     7141 gtcgatgccc tggtcggctt tcttcccagc agcaacccgg ttctgggcaa taaagtcgtc
     7201 gtgacaggcg aagcgacaat caacctgcag aatacgctcg ggaacggcga aacgatgcgg
     7261 ctcgaatggc aacagctcca ggcaggctcg ccccggctga acctgctgta tcaacaaccg
     7321 ttcgtgttca gctcaccgtt tggtctgacc acctctttcg atctgtacaa gcaggactcg
     7381 agctatgtca atatcaattt tgtcgtcggc gcccaatata ccttctccgc caaccagagc
     7441 gggtctgtct ttctccagct ggcatcgtcg aacttactga acgtagacac cgtcgcggtc
     7501 atcgcgacgc atgccttgcc gccgcaggcc gatatcagct ccacgagcct cgggctgacc
     7561 tatagctaca acaatacgaa ttaccggttc aatccacgcc ggggaaacga aatgaatttt
     7621 cttggttcgg caggcacgaa gcgggtccat gagaacggcc agatcgccgg cctcagcgat
     7681 ccctcggaca gcggttttaa gtttgcaagc ctctatgata ccgtaaagct ccattccttt
     7741 cagttcctcg tgaaatggga aggggcccac tatttcccaa tcggccgggc ggcgacgata
     7801 aaactcggcg tcaacgccgg ggtctacagc agcccaaata cgtatcgtaa tgatttgttc
     7861 atgatcggcg gctaccggct gctgcgcggt ttcgatgagc aaagcatcct ggcatcagaa
     7921 tacgcggtgg gaacgctgga ataccggtat ctgacgggtt tgaactcctt tttgtttgcg
     7981 ttcgcggacg tcgggtatgc ggcgaaccat gtgtccgatt accatttgaa tagcctgtac
     8041 atcggcaccg gcgccgggat ggcttttgaa accaaggcgg ggattttcaa tatgtcgctt
     8101 gccgtgggaa ggaccgatac atcgccgttc aatttccacg acaccaaaat tcatcttggc
     8161 tatgtcagct ttttttagca aggatgtata tttttgcacc agtaattaag aaagacttga
     8221 tctttaccag aatttccatt atcctcttaa cctgcctgct tgccatcggg gggctgcagg
     8281 ctcagaccga ttcagcacct ccgcccgctg ctcagcacgg acgggcacag gatacaggct
     8341 cgttgccagc cgcccggccg ctaccacgcc gggattccgc ccgccgcgcc cgcccggatt
     8401 ccctcaacca acttcgaagg ggccccgcca ccggcgctgc ccgggatacg gcgacttcat
     8461 cggtgaaggc tacccgcagc gacacccttc cggggaacgg gggcgttgct ttatttcctg
     8521 gagccggtca gtctcctgcc gcaatgggct attggcagaa agtgcttgcg gaaaatccct
     8581 atctgccatt caccggccag ccggtggccg agacatttga ggtacatcgc tccaatggga
     8641 aagaaagcat gttctatctc atagtcgcca tcctgttcta ttttgggttg atccgggtct
     8701 tcttcgaaaa gtatttcaat aatctcatga ccctgttttt ccgggtttca ctccggcagc
     8761 agcagatccg cgaacaggtg ctgcagacgc ccctgccctc cctgatgctg aatattctct
     8821 tcgtcctgtc cgcaggcctg tatgcctgct tcctgctgca ttattcgaag ttcggcgccg
     8881 ggatccgttt ttggctcctg tacatggact gcatggcgct gctggcggcg atctacctcg
     8941 tcaaattcct cgtgttgaag tttatcggct gggtcttctc gatttcccgg gcaaccgaca
     9001 cctatatttt tgtagtcttt ttggtcaaca aaatgatggg aatgatgctg cttcccttct
     9061 tgatcgtgat catcttttcc ggccagcaat ggcgtgaagt gtttataacg atctcattgg
     9121 caatgatttt tgtcttgtgg gcttatcgta tcctggcatc cttccgtcct gtccgaaatg
     9181 aaataaaact aacgccgttt tacttttttt tgtacctttg cgccttcgaa atagcacccc
     9241 tactgttgat ttacaaggtg ttattgacat atttggaaaa agtgtattaa atttgcaaaa
     9301 ccggctgcag accggtaatc ccgaggggca cacacatgac taagaacgag gataaaaaaa
     9361 agattttaat cactcaaccc agacctgaaa ccgacaagtc gccgtatttc gagttagcca
     9421 aaaagtacga cgttgagtta gcattttttc ctttcattcg cctggagccc cttcctgcaa
     9481 aggatttccg caaacagaaa atagacatca cccaatttac cgcagtcatc ttcacgagcc
     9541 ggaatgcaat cgatcatttt ttccgcatgt gcgaggagat gaagaccagc gtttcgcagg
     9601 acaccaaata tttctgcatt accgaagccg tggcgttgta tctgcagaag ttcatcctct
     9661 accgcaagcg caaagtgttc tacggcgcgg acggtctgaa caagagcatg tttgatgtca
     9721 tcaacaagca caaggagaac gaaaaattcc tgtatccatg ttcggagaat cagcaggata
     9781 acgagatcgt cagctggctg aagcaaaaca actgcgggtt tgcgacgccg ttcatgtacc
     9841 ggacgatcag caatgatgta aaagaaatcc tccaccagag cgaatacgat atcatttgct
     9901 ttttcactcc cagcggcgtg aaaagccttt tcgacaattt tcccaagttc aagcagaacg
     9961 gcacgaagat cggcgccttt gggaacaata cctcccgggc cgttgaagag gcgggcctca
    10021 cgctggatat caaggctccc cagccgcagg cgccgtcgat ggtctcggcg ctggaaaaat
    10081 acctggcggt gaccgtaaaa aagaaataga ccgtcgatct agcgcgaaaa ttgcatacct
    10141 tttagctccg gcacatcaag gaaacgtcat gcctaatcga ctcgccacgg agaccagccc
    10201 ttatttattg cagcatgccc acaatcccgt ggactggtac ccctggggcg acgaagcatt
    10261 ggccagggct gcccgggaaa acaaacccat cctcgtcagt atcgggtatt ccgcctgcca
    10321 ctggtgccac gtaatggaac gcgaaagttt tgaagaccag gagaccgccg ggatcatgaa
    10381 tgatcatttc gtcaacatca agatcgatcg ggaggagcga ccggacctgg atcatattta
    10441 tatggacgcg gtgcaggcaa tcaccggcag cggtggctgg ccgctgaatg ttttcctcac
    10501 ttccgacggc agacccttct acggaggcac ctatttcccc ccgcgtcctg tccacaaccg
    10561 gccttcctgg aaggaagtgc tggcggcggt tgcccgttct tacagcgaaa gaagagcgga
    10621 tttggaaaag caggccgggg cgctgacgag gcacctggcg gtcgccgcat cccttggcgc
    10681 cgggaacgaa ccggcggcag ccgtgggagc ggatggggaa ttcacgcccg atctgctgaa
    10741 acagatccgc gacaatctcc tttcaacggc tgacacccgg gacggcggat ttggcggggc
    10801 gccgaagttt ccgcaaacgt tttcgatccg gttcttactc cactattatt attttaccgg
    10861 cgaccggggc gcccgccagc aagcctgcct gagcctggac aagatgcttg cggggggaat
    10921 cttcgaccag cttggcggcg gtttcgcccg gtattcgacc gacagcggct ggcgggtgcc
    10981 gcactttgag aagatgctct atgacaacgc gttgctggtc atcgccctgt gtgatgccta
    11041 tcagcttacc ggcgagcccc gctaccggga agggatagag atgacgctgg agtttgcgga
    11101 tcgcgaacta tccaacggcc ggggcgggta ttattcggcg ctggacgcgg acagcgaggg
    11161 cgaggaagga aaatattatg tctggaccag ggatgagatc gaggcggtgc tcggcgcgga
    11221 tgccggggtt ttctgtgcgc ggtttggcgt cacggctgaa ggcaactggg agggaaagaa
    11281 tatcctggtg cgggcccggg atgttccctc ggatccgggc gtcaaagcgc gtctggatgc
    11341 ggcttgccgc cgcctcctgg cccaccgggc gcaaagggtg cggccgggcc tggacgacaa
    11401 gatcctgctg ggttggaacg ccctcatgaa tattgcctgc tcgcgggcct ttgccgcgct
    11461 tggaaaggag cagtaccgcg accgcgcgcg gacgaatatg gagttcctgc ggacaaatct
    11521 gaagggcggc atgatccatt tgtatcacca ttcgtttaag gacgggccca aatttcccgc
    11581 gttcctcgat gactatgcct tcctcatcca ggccttaatg gaattgcagg agattaccgg
    11641 ggacgccggc tatctcgacg aggccaggga aataacggaa atggtcattg accatttcgg
    11701 ggaagagggt acaccatttt tttacttcac ccacgaccag cagaacgacc tcatcctcag
    11761 aaaaaaagag atttatgacg gggcgacacc ctcgggcaat tccataatgg cattcaattt
    11821 gttttatctt tccaagctct tcgacgtacc tgcctggaca gatcgggccc ggcaaatggc
    11881 cggaggggtg aaaaagctgg tgaccggtca ccccggctct ttcggaactt gggcaaccct
    11941 gttccaggcg cttacgtata ctgttccgga gatcgtgatc accgccaggc ggccggaaat
    12001 tgcccggaag gacttcttga aagagtttgt tccctaccgg gtttttcaat cttcgtctca
    12061 ggaaaatacc cattacccct tactgcggga ccggcctgcg ggggaagtgc cgcagttctt
    12121 cgtatgtgtg gatcgtgcgt gccaactccc tgtaaatgag ctatctgcat taatccgcct
    12181 cctcgaaaat gtgaataaaa ttccatcgaa atcgtacaat aatccgatat aggttgcgtt
    12241 tcaatgaata gcgtcaatcc ctcgtcctgt gaaagcaatt tgtccggatg aaagctttga
    12301 cattcgaaaa attaatttta aacttgtagc gagatgttgt cagaatgttt ttttggccca
    12361 atacccttta atattatgaa aatgaaaaac ctatccgttt tgacagcttt tagcgccatc
    12421 tttctgctgg caagctgtaa actaggtgga aaatccggca aaggattgct ggatgacggg
    12481 caactgcacg gcgtcgcacc tgcaactgcc tataatctca agaaacctct tggcatggtc
    12541 tatattccac ctggaacctt tcatatgggg cccagcgacg aggatatgaa ctatgctttc
    12601 actgcccgca accttcaaat ttcgatcagt ggattctgga tggacgcaac ggagatcacc
    12661 aataacgagt accgccagtt taccaactgg gtccgggact cgattgccgc taccctgatg
    12721 caatacggca aggatgtgga tggccggttc gccattgact ggaaaaaggc cgcgaccatc
    12781 aagtacgccg acaaggctac gatcgaaaag atcaatcaga tgatcgtgga gccggataac
    12841 cgcattttcg gcaggaaaga gatcgatccg aacaagatcg tctaccattc cgaaactttt
    12901 gacctgaatg cggccgccca gaagatcaat gaaggcaagc cccgttcgca gttcatcgaa
    12961 aagaaagaca tcaaggttta tcccgatacc ctcgtctgga tccgcgactt ttcctattcg
    13021 tataacgagc cgatgaccaa gcggtattac tcgcacccgg cattcggcaa ctaccccgta
    13081 gtgggcgtaa actggaacca ggctagcgct ttttgcgact ggcgcaccca gtacctgaat
    13141 gcctacctgg aaggcaagca tatggccacc gagtccaatt tccggctgcc tacagaggca
    13201 gaatgggaat acgcggcccg cggcggccgg tcccaatcca tgtatccctg gggtaactac
    13261 tacctccgga acaaacgggg ctgtcttttg gcgaacttca aacccggccg tggcaactat
    13321 cccgaggacg gcggcttcta tacggtaagg gctgatgctt actggccgaa cgatttcggc
    13381 ttgtattgca tggcgggaaa cgtagccgaa tggacctctt ccttctatta cgaaggggca
    13441 tccaatttcg tccacgatat gaacccggat atccgttgga acgcaaagga ctcggatccc
    13501 ccccgcatga agcgtaaggt gatccgtggc ggcagctgga aggatgtggg tctttacctg
    13561 cagaccagtg cccggaccta cgagtaccag gacacggcta aatcctacat cgggttccgt
    13621 tgtgtgatcg atctggcgcc gaccttaagc gggaatcgca ggtaattcat ttccgtgaag
    13681 caaaattttt aaccagaaat agcgttttga ttttgaaaac agacaaaaaa tcaaaaggag
    13741 ctttttttcc ttaacccttt aaattccttt aacaacaatt taaattaatc actatgagcg
    13801 ctggagtatc aaaatctacc gaaagactga tcaatatcgt tgtttgcgtc ggcgcagcag
    13861 tcgtcatttt tggcgcgctt cagaagatcc tgcataccga gctggctaac ttctttttga
    13921 cagccggtct gctgaccgaa tccctgatct tcctgatcta tgcgttcctt ccgcctcccg
    13981 gaggtgaaat gcatgccctg gtcgaggcgc tgcccaagct ggccggtggt accagtggaa
    14041 acccggcgct ggacaagatg gacaagatgc tccaggaagc cgatatcacg ccggcaaatc
    14101 tgggccggct ctccgaaggc ttcaagaaat tcgggaccac cgttgaaaag atcaccgacg
    14161 tttccggcgc gctgaccgct actacagaat atacgaacaa gactaaggaa gcagctgcgg
    14221 cgctggacaa tatgaaaggc gcttatcaga atgccaccaa taccatctcg agctttaacg
    14281 cagcagcaga cagcacgaag caattccatt cgcaggtaca ggtgctgacg aagaacctgg
    14341 gctcgctgaa tgcaatctat gagctggagc tgcaggacac caataaccac ctgaaagcca
    14401 tgaacagctt ctacggtaac ctggtgaaag cttccgagac gatgcaaggc agcgtaaccg
    14461 atgcgaaaaa gacacaggaa cagatcgccc tgctcgccaa gaacctgggc aacctgaacc
    14521 atgtctatgg taacatgctg agcgccatgc aaggacgtca ggcgtaaccg gccgttcgaa
    14581 accaaacaag ccaagcccgt tttgtccggc cccgcatgaa cctgaactgg acgatgtcca
    14641 gtgatagaca tcaaagaacg atgaaaaacc attaatgcat tttaattact gacccatgtc
    14701 tatacctaaa gaaccgcggc agaagatgat caacatgatg tatttggtgc tgacagccct
    14761 gctggcactg aatgtatccg tggaggtatt gaacgcgttt aaaaccgtga acaccagtct
    14821 cgagaactcg aataatgtcg tgagccaaaa gaacaacctg acttataccg ccttccagaa
    14881 gaacctggaa gacccatcga cgaaggccct ggccgagatc tggtccaagc cggctttcca
    14941 ggttcaatcc tatgcagccg ctttcagcac ttacctggaa gcgttgaaga cccagctcca
    15001 gaaggagtcg ggattgaaga cccaggacgg taaggcaatt tataatgagg gcgacctgga
    15061 cgccgccacg cggatgttcg accagggtgg ccatggcaag aagctgtatg attcgctggc
    15121 gaatttcaga aattacctgc tcagcgaact ggatccgaat aacccggcct atgccaatgc
    15181 aaacctttca gagctgacca aacaggaaat gatcacgacg aaggcggagt tcgccaagcg
    15241 cctgcccatc gaccttcgca tcccgaagtc ggaaaccggc gaagccgaga agaaggatcc
    15301 ggcagagaac tggaccatcc gctacttcca tatgac
//
LOCUS       DATGKE010000096         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_97292, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000096 DATGKE010000000
VERSION     DATGKE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97292"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VKU83_01725"
     CDS             complement(<1..1245)
                     /locus_tag="VKU83_01725"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLI92292.1"
                     /translation="MKIDFYLRYYTQPGQSICLSGNWTLLGEGDPEKSFPLQYVNSDF
                     WHASLEVEDFPADPLVYHYILRGPDGILTEEGGNDRVLEAPGRGTNEIQLIDTWNYAG
                     EFENVFYTSPFREVLLPHHKAGKKTRPKGGYTHIFRVKAPLLQSGESVCLLGSGATLH
                     EWNPQEPIILSREGNWWSAYLTVPPESFPMEYKYGVWNKKAKQFLRYEAGPNRNLPGD
                     ARPNKVSILHDGFVHEPNNNWHGAGVSIPVFSLRSKKSMGVGEFTDLKALIDWAARCG
                     LRMVQILPVHDTTATHTWIDSYPYAAISAFALHPIYLNLEKCAGRKYAELIKPLKKRQ
                     KELNKLPELDYEAVMKIKLLTVTELYELQKEEFKTDADFLEFFERNSSWLIPYAAFCY
                     LRDKHKTPDFTGWKVHSHYDAAA"
     gene            1402..1557
                     /locus_tag="VKU83_01730"
     CDS             1402..1557
                     /locus_tag="VKU83_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92293.1"
                     /translation="MERKKKNTGIYWVLFLISVAGFIYAVDTHFEYITMILPFGCTFF
                     VKALDII"
     gene            1602..3446
                     /locus_tag="VKU83_01735"
     CDS             1602..3446
                     /locus_tag="VKU83_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92294.1"
                     /translation="MDLAQFYSSAAGDCREQLRLIRRKTNAVSLLRLGLFGCLGLSLW
                     FLFNAGSPWLLAAAVASLTAFIASVNWYFRLKDQRLLWEKLVFVNENEKGILQGQENG
                     FPDGAGFLDQENYLDDLDVFGPSSVFHALNRTTTLHGTGALVDLLRQGLLSPAAIREQ
                     QQAVKRLAEQPALRRLLTAKGLLAGEGKGDLRTLSRWLNMKPVVYPLVWLRILISVLT
                     LVNLAVLVYSLASGRYIALVAAIVVDRLIIAYFGREVSEQQRLISHKQEVFAQYADIL
                     AVFSGVETDQAPVLDGLRSRAREAHGAIRRLSRLTSFFDQRMNLMVNLVLNSLAFYDL
                     QCMVGLERWKQRNKEAFPGWIEAVGRIEALNSLATFAFNHPGYVWPSPQEGWPDGDLR
                     PSGGLTSVTPQPGAGEGLFIEARQLGHPLIPAKRRIANDFRIGREERLILVTGSNMAG
                     KTTFLRTIGVNVLLAQCGAPVCAETFSFTPMQLLTSLRISDSLQEQTSYFMAELKKLQ
                     RIVGRLETGVPTLVLIDEILRGTNSEDKNYGSDQFARKLTGYRCLSLFATHDLALGQL
                     ETDLPGIVANYCFESVIEDGDLIFNYRLQRGVARNRNASFLMKKMKII"
     gene            complement(3561..3785)
                     /locus_tag="VKU83_01740"
     CDS             complement(3561..3785)
                     /locus_tag="VKU83_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92295.1"
                     /translation="MKSDLMHSRVQLEKDILDRLVTEVKETVATDVQLPKVERTAPFS
                     VLKLWNMRRNARYATIIGNRPPRIVNGIGY"
     gene            complement(4062..5249)
                     /locus_tag="VKU83_01745"
     CDS             complement(4062..5249)
                     /locus_tag="VKU83_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92296.1"
                     /translation="MKAGLAFAPLTIISLFCQAQIEYRPGYFIRNDSTKVSCLIKDYD
                     WRNNPTGITWKSVATGTSATMDRDSILEFGVGNAVFRRFEIDVDVSAGENDDPGNSPL
                     PTFQRETVLLKLLVQGRASLYRYSNKGLTLFYMVVNNPTPQPLVYKQFLSQSNLELTN
                     TGYITQLQDSLRCTAPGFPDPAAVRYEERSLVRFFVAFNNCTHSAVTDYSAKTSGSPV
                     HPSIRAGIDLTSLTLDDQTGSVDEQKNLGNKLSFRIGAELEIPLNFNRNKWAVVVEPA
                     YRYYSAQTAEGSSGFQVNYRAIQLGIGGRYYLFSGPSSRWLLTGLLVADFPVNSSVEY
                     LQESLRVRTHAGASASAGYLYRERAGVELKYASPEQILDNYFYLHAKLAGTVSLVFSY
                     RIW"
BASE COUNT         1177 a         1351 c         1502 g         1313 t
ORIGIN      
        1 ggcggccgcg tcataatggc tgtggacctt ccagcccgta aagtcaggcg ttttatgttt
       61 atcgcggaga taacagaagg cagcgtacgg gatgagccag ctgctgttcc tttcaaagaa
      121 ctccagaaaa tctgcatccg tcttgaattc ttctttctgt agctcgtata actcagtaac
      181 tgtcagcaac ttgatcttca taacggcctc gtaatccagt tctggcaact tgttgagttc
      241 cttttggcgt tttttcagcg gtttgataag ctcggcatac ttgcgccccg cacatttctc
      301 caggttaaga tagatcgggt gtagggcgaa tgccgagatc gccgcataag gataagaatc
      361 gatccaggta tgcgttgcgg tcgtatcgtg gaccgggaga atctgcacca tgcgaagccc
      421 gcatctggcg gcccaatcta tcagggcttt cagatccgta aattccccga cgcccatgga
      481 ctttttgctt cggaggctga acacgggaat ggagacgccg gctccatgcc agttgttgtt
      541 gggttcgtgg acaaagccgt cgtgaaggat gctgacctta ttgggtctgg cgtcaccagg
      601 aagatttcgg ttggggcccg cctcataccg aaggaattgt tttgctttct tgttccagac
      661 gccatattta tattccatcg ggaagctctc cggcggaacc gtcaggtagg cggaccacca
      721 atttccttcc cttgacagga ttatgggttc ttgcggattc cactcatgga gggtggcgcc
      781 gctgccgagg aggcagacgg attctccgga ctggagcagc ggggctttaa cgcgaaagat
      841 atgcgtatag ccgcccttcg ggcgggtttt ttttccggct ttatgatgcg gcagcaagac
      901 ctcccggaaa ggcgaggtat aaaatacatt ttcaaattca ccggcataat tccaggtgtc
      961 gatcaactgg atctcgttcg ttccccgccc cggtgcttcg aggacccggt cgtttccgcc
     1021 ctcttcggtc aggatgccgt ccggccccct caggatataa tgatagacca gtgggtccgc
     1081 agggaaatcc tccacttcga ggctggcgtg ccaaaaatcg ctgtttacat attgaagggg
     1141 aaaactcttc tccgggtcgc cttcgcctaa caacgtccag tttccggaaa ggcagataga
     1201 ttggccgggc tgcgtataat aacgcaggta aaaatcgatc ttcatggttt aaagcaactt
     1261 tttcttaagc aggcaaccgt aatgtgtatt ttttacatta tgccgctaat ttagggtaat
     1321 ttttcattat gaatcaacta aatccgtttt tttgcagcaa ttgacctaaa ttcgcaaaaa
     1381 tagtttcaaa aaagattgtc catggaacgc aagaagaaaa ataccggaat ttactgggtg
     1441 ttgtttttga tttccgtcgc cgggttcata tatgccgttg atacccattt tgaatacatc
     1501 acgatgatcc tccccttcgg ctgcactttc tttgtcaaag cgttggatat catctagtcg
     1561 cggaaggcat tttctttcct ttcattatct tcgaaaatgc catggattta gctcaattct
     1621 attcgtctgc cgcgggggac tgccgggaac agttgcggct gatccgcagg aagaccaatg
     1681 cggtctccct gttaaggctg ggtttgtttg gttgcctggg actatccctg tggttccttt
     1741 tcaatgcggg ctccccatgg ttgctggcgg ccgcggtggc atccctgacg gcgtttatcg
     1801 cctcggtcaa ctggtatttc cggctgaagg atcagcgctt gctctgggaa aagctcgtct
     1861 tcgtaaatga aaacgaaaaa ggcatactcc agggccagga aaacgggttc ccggacgggg
     1921 caggcttcct ggaccaggag aactacctgg acgatctcga cgtttttggt ccgtcatccg
     1981 tctttcacgc gttgaaccgg accaccacgc tccacggtac cggagcgctc gtggatctac
     2041 ttcggcaggg cctgttatcg ccggcggcga tccgggagca gcagcaagcc gtcaagaggc
     2101 tcgcggaaca gcctgcattg cgtcgtcttc tgaccgcaaa gggtttgctg gctggagagg
     2161 ggaagggcga cctgcgaacc ctgtccagat ggctgaacat gaagcccgtg gtctacccgc
     2221 tggtttggct gcggatcctc atttccgtgt tgacgctggt taacttggcg gtgttggtct
     2281 actcgcttgc ctcgggcagg tatattgcgc tcgtcgctgc gattgtggtg gaccggttga
     2341 tcatcgctta tttcgggcgc gaagtgtcgg aacagcaacg cctgatcagt cataaacagg
     2401 aggtttttgc gcagtatgcc gatatcctgg cggtattcag cggtgttgaa acggatcagg
     2461 cacctgttct tgacgggctc cggtcgcggg cgcgggaagc gcacggagcc atcaggcggc
     2521 tgtcgcggct gacctccttc tttgaccagc ggatgaacct catggtgaac ctggtgctga
     2581 acagtctggc gttttatgac cttcaatgca tggtcggcct ggagcgatgg aagcagcgca
     2641 ataaggaggc atttcccggg tggatcgagg cggttggccg gattgaggct ttgaattcgc
     2701 tggcgacatt tgccttcaac catccgggct atgtgtggcc gtcgccgcag gaaggatggc
     2761 cggacgggga tcttcggccc tcaggcggat tgacttccgt gacgccgcag ccgggtgccg
     2821 gggaagggct gttcattgaa gcccggcaac tggggcatcc gctgattccc gcgaaacggc
     2881 gcatagccaa tgactttagg atcggccgcg aggaaaggct tatcctggta acgggatcga
     2941 atatggccgg caagacgacc ttcctgcgaa cgatcggggt caacgtattg ctggcgcagt
     3001 gcggcgcgcc ggtttgcgcc gagacttttt cctttacgcc gatgcagttg ctgacgtcgc
     3061 tccggatcag cgattctttg caggagcaga cctcctattt catggctgag ttgaaaaagc
     3121 tgcaacgaat tgtcgggcga ttggagacgg gagtccccac cctcgttctc attgacgaga
     3181 tcctgcgcgg tacgaattcg gaagataaga actacggttc ggatcaattt gcccggaagt
     3241 taacgggata tcgctgcctg tcgctttttg ccacgcacga cctggcgctc gggcagttgg
     3301 agaccgactt gccgggaatt gtcgcgaact attgttttga aagtgtgatc gaagatggcg
     3361 acctgatctt caactaccgg ttgcagcgag gcgtggcgcg caaccggaat gcatcgttcc
     3421 tcatgaaaaa gatgaaaatc atttagcggg gcgcggatgg ccccgggttg gcgacaataa
     3481 gcactcacga tcaatgagag tggagctgta aaaaaaagcc ggcccctggg gaccggccgt
     3541 gctggaaaca accttacata tcagtaaccg ataccgttga cgatgcgagg gggcctgttg
     3601 ccgatgatag tggcgtagcg ggcgttccgg cgcatgttcc acaacttgag gacgctgaaa
     3661 ggagcggtcc tttccacttt cggcagttga acatccgtcg caaccgtttc tttaacttcc
     3721 gttaccaatc tgtcaaggat gtctttttcc aattgcactc ttgagtgcat cagatcactt
     3781 ttcataacta tctgttttta attaatctac aatttctcgg agcaaataac taagcgggaa
     3841 aaactaattt ttggccttgg gccggaggcg ctaccgccct cttgtacact ttagaatcgt
     3901 tgaagaagtg tgtcggcaga gatctaacaa tatggcaaac taaacgcgga gcttagttgc
     3961 tcgatttcgg ttgcaaagat acgaaaaatt ggtgatcttc caaatccgcg gaccgaaagc
     4021 ctgccgccca aacagcggat ttacgccgga gtgtgcagag atcaccatat cctgtaggaa
     4081 aagaccaatg aaacggttcc ggcaagtttc gcgtgcagat aaaaataatt gtccaggatt
     4141 tgttccggac tggcatattt gagctcgacg ccggctcgtt cccgataaag atagccggcg
     4201 ctggcggatg cgccggcatg cgtccttaca cgcaggcttt cctgtaaata ttccacggat
     4261 gaattgaccg ggaagtcagc gacaagcagg cctgtcagca accaccgcga ggaggggccg
     4321 gagaacaaat agtatctacc gccgatgccc agctggatcg ctctgtagtt gacctggaaa
     4381 ccggaactcc cttctgccgt ctgggcggag taataacgat aggccggctc gacgacgacc
     4441 gcccatttat tccggttaaa attgaggggt atttcaagtt ctgctccgat cctgaagctc
     4501 agtttgttcc ccagattctt ttgttcgtcc acggaaccgg tctggtcgtc caaggtcaaa
     4561 ctggttaggt cgatgccggc acggatgctc gggtgcaccg ggctgcccga cgtcttcgcc
     4621 gaatagtcgg taacggcgga atgcgtacag ttattgaaag cgacaaaaaa tctgaccagg
     4681 cttcgctcct cataccgtac tgctgcgggg tctggaaaac caggcgccgt acagcgtagg
     4741 ctatcctgca actgggtgat atatccggtg ttggtgagct cgagattgct ttgcgaaaga
     4801 aattgcttgt aaacgagcgg ctgaggcgtc gggttattga ccaccatata aaacagcgtc
     4861 aaccccttat tgctgtaacg gtaaagagat gcccgccctt gaaccagcaa tttcagcagg
     4921 acggtctcgc gctggaaagt gggcagcggc gaattgccgg ggtcatcgtt ttcgccggcg
     4981 gaaacatcga cgtcaatttc gaagcgacga aagacggcgt ttcctacccc aaactccagg
     5041 atactgtcac ggtccatagt ggctgatgtt ccagtcgcaa cgctcttcca ggtaattccg
     5101 gtcgggttgt tacgccagtc gtaatccttg atcaggcacg agactttggt gctgtcgttt
     5161 cgtatgaagt atccgggccg gtactcgatc tgggcctgac aaaacaggct tataatggtt
     5221 aacggcgcga aggccaatcc ggctttcatc gtggtggttt agtatcccca agatagggat
     5281 aaaccggtaa aagcgaaggc ccctccggaa aaaaggggcc ttcgcgtcaa aacgtatatt
     5341 tgc
//
LOCUS       DATGKE010000097         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1328914, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000097 DATGKE010000000
VERSION     DATGKE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328914"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VKU83_01750"
     CDS             complement(<1..185)
                     /locus_tag="VKU83_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92297.1"
                     /translation="MLKNKLFSSYWFRSAFFSLLQRFSITLFGMVSYMLLAKRGLSVA
                     EMGVYAIFLVVITVFENT"
     gene            complement(204..1103)
                     /locus_tag="VKU83_01755"
     CDS             complement(204..1103)
                     /locus_tag="VKU83_01755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI92298.1"
                     /translation="MESSPLVSIITVNYNSTEVTAELLRSIKKVNYPSLEVIVVDNAS
                     REDPTQRLREVDPDVKVMVSATNRGFSGGNNLGMRAAGGAYFFLVNNDTEFTPGLIEG
                     LLDVFRQHEDAGMACPKFHFYYHPAIIEYAGYNRLNVLKGSCTMIGNKEKDEGQYDVE
                     RSTPFAHGGAMMVPAKVVQEVGEMPECYFLYYEELDWSEQIRRKGYSIYYQPRALIYH
                     KESVSVGKKSLVKTFYHTRNRILFMRRNVGPAALAVFTLYFMFFSIPKNSVQFLARGE
                     GKHFKAFWRGIFWNLAHYRIKNL"
     gene            complement(1093..2307)
                     /locus_tag="VKU83_01760"
     CDS             complement(1093..2307)
                     /locus_tag="VKU83_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI92299.1"
                     /translation="MASFFEYLFYFVFFYFAITVFYLFSLSVGARIFSRRLAAINPSG
                     AGGGKKRIAILVPAYKEDGIIVSTAASHLGLDYPRERFDVYILADSFREDTLAELRRM
                     PVEVLVMKFDKSTKIKSLNSALERMQKDYDIVVISDADNVLARDFLKKIEASFDAGEM
                     VVQGRRVAKNMDTSYALLDACSEAINNHIFRKGPNAYGLSAPLIGSGMAFDFSIIRDL
                     LRGIDSVVEDKVLQLRIAQRRIFIRYREDALVFDEKVDSSHAFRQQRRRWVSGQFINL
                     KESFLASIEQLLRGNISYFNFGIVNNLVLPRAFLLVMLPALVVAGFFISPFWGIASTV
                     IFLLYATSLLLAIPRALVNRDLFAALLKLPGAILVMAGALLRMKKSHKVFIHTVHTKT
                     EISNSLFNENGK"
     gene            2370..3557
                     /locus_tag="VKU83_01765"
     CDS             2370..3557
                     /locus_tag="VKU83_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI92300.1"
                     /translation="MHVWLGAFIISCFLVVYNYIGYAFPIYLWNRLRGPLQPPQPKSP
                     EEWPAVSFIVAAFNEQDCIREKILNSLAQDYPGKKLEFIFVTDGSTDDTPRIVAAFPE
                     IKGLYQPERNGKSAAVNRAVAAASNNILIFSDANTVLNVDAIRNLARHYLDPQVGGVA
                     GEKKVLSTTAAAREVGEGEGLYWKYESFLKRQDSAFYSVVGAAGELFSVRSALYEPLP
                     HHIILDDFVLSLNVAKKGYRVVYEPGACATELPSLSMADEHRRKVRIAAGGFQAISLL
                     TPLLAFWRRPRLSYLYISHRFMRWAVSPLCLIVAYVSNCVLALGTSVAIYRWLLGAQT
                     AFYGMAALAARFPILKEKSRLLKLGSYFVFMNVSVVQGFFRFVRGKQAAAWEKARRVQ
                     SAA"
BASE COUNT          840 a         1094 c          925 g          849 t
ORIGIN      
        1 gtattttcaa agaccgtgat gacgaccagg aagatcgcgt acacgcccat ctccgcaaca
       61 gacaggcctc tcttggccag caacatatag ctgaccattc cgaataacgt gatggaaaat
      121 ctttgcagga gactaaagaa cgccgacctg aaccaataag aagagaatag tttgtttttc
      181 aacatgatgc gacctgaggc cggttatagg tttttgatcc ggtaatgcgc caggttccag
      241 aagatcccgc gccagaatgc cttaaaatgc tttccctccc cgcgcgcaag gaactgcacg
      301 ctgttttttg ggatgctaaa aaacataaaa taaagcgtaa acacagccag cgccgccggc
      361 cccacattcc ttcgcatgaa caggatgcgg ttgcgggtgt gataaaacgt cttcacgagg
      421 cttttcttgc ccaccgacac cgattcctta tggtagataa gggcccgtgg ctggtagtag
      481 atgctgtatc ccttgcgcct gatctgctcg ctccagtcca gttcctcata atacaggaaa
      541 tagcattccg gcatttcccc cacctcctgc acaactttcg ccgggaccat catcgctccg
      601 ccatgggcaa agggcgtgct ccgctccaca tcgtattgcc cttcgtcttt ttctttattg
      661 ccgatcatcg tgcaactgcc cttcagcaca ttcaaccggt tatagcccgc atattcaatg
      721 atcgccgggt gatagtagaa atgaaatttc ggacatgcca tgcccgcgtc ctcgtgctgc
      781 cgaaaaacgt cgagcaaacc ttcgatcagc ccgggcgtga attcggtgtc attgttgacg
      841 agaaaaaagt aggcgccgcc ggccgcccgc atgccaaggt tattgccgcc cgaaaaaccc
      901 cggttcgtcg cgctcaccat caccttcacg tcggggtcaa cttcccgcag ccgctgcgtc
      961 ggatcctcgc gcgacgcatt gtccacaacg atgacctcca gcgaaggata gttcactttc
     1021 tttatcgatc gcagtaactc cgccgtaacc tccgtgctgt tgtaattcac ggtgataatg
     1081 gagaccaaag ggctactttc cattttcatt gaacaaggag ttgctgatct ctgttttggt
     1141 gtgaacggta tggataaaaa ccttgtgtga ttttttcatc ctcaggagag cccccgccat
     1201 cacaaggatc gcaccgggga gcttaagcaa agcggcgaac aggtcgcggt tgacgagggc
     1261 ccgcggaatg gccagcaaaa ggctcgttgc atatagcagg aagatgaccg tcgaagcaat
     1321 gccccaaaac ggcgaaataa aaaaacctgc cacgacgagc gcagggagca tgaccaataa
     1381 aaatgccctg ggcagcacca ggttattgac aatcccgaaa ttaaaatagc tgatgtttcc
     1441 cctgagcaat tgttcaatcg aggcgaggaa cgattctttg agattgatga actgcccgga
     1501 cacccaccgc ctgcgctgct gcctgaaagc gtgggatgaa tccactttct catcgaacac
     1561 cagtgcatct tcgcggtagc ggatgaatat gcgccgctgc gcgatccgaa gttgcagaac
     1621 cttgtcttcc accaccgagt caatgccccg cagcaaatcc cggatgatcg aaaaatcgaa
     1681 cgccatcccg gatccgatca gcggcgcgga taacccatag gcgtttggcc ccttgcggaa
     1741 aatgtgattg ttgatcgcct cgctgcaggc atcgagcaag gcataggacg tatccatatt
     1801 cttggccacc ctcctgccct gtacaaccat ctcgcctgcg tcgaagctgg cctcgatctt
     1861 tttcaggaaa tcgcgggcaa gcacgttgtc ggcatcactg atcaccacaa tatcgtaatc
     1921 cttctgcatt cgctccagcg cgctgttcag cgacttgatc ttcgtgcttt tgtcaaattt
     1981 catgacgagc acctccaccg gcatccggcg cagttctgcg agcgtgtctt cccggaaaga
     2041 gtcggccagg atatagacgt cgaaccgttc gcgggggtag tcgaggccca ggtggctcgc
     2101 cgctgtcgat acgatgatcc cgtcttcctt gtatgccggc acaaggatcg cgatccgctt
     2161 cttaccgccc cctgcgccgg aggggttgat cgccgccagc cgccggctga aaatccgggc
     2221 gccaacggac aagctgaaca gataaaagac cgttatggca aagtagaaga agacaaaata
     2281 aaaaaggtac tcaaaaaatg aggccataac tttgaaatat atttacgagt ctgtcagtcc
     2341 gggtcctaaa attagtcaaa tttccctata tgcacgtatg gcttggcgcc ttcataatca
     2401 gctgtttcct agtagtatat aactatatcg gctacgcgtt ccccatttac ttgtggaacc
     2461 ggcttcgcgg gccgctccag cccccgcagc cgaagtcgcc cgaagagtgg ccggccgttt
     2521 cctttatcgt ggcggccttc aacgaacagg attgcattcg ggaaaagatc ctcaattccc
     2581 ttgcccagga ctaccccggc aaaaagctcg agtttatctt cgttaccgac ggctcaacgg
     2641 acgatacccc gcggatcgtt gccgccttcc cggaaatcaa gggcctgtat cagcccgaaa
     2701 ggaacggaaa gtccgccgcc gtcaaccggg cggtcgccgc cgccagcaat aatatcctca
     2761 ttttcagtga tgccaacact gtcctgaatg tcgatgccat ccggaacttg gcccgccatt
     2821 acctcgatcc gcaggtcggc ggcgttgccg gcgagaaaaa ggtgttatcc actacggcgg
     2881 ccgcacgtga agtaggcgaa ggcgaaggcc tttactggaa gtatgagtcc ttcctaaaac
     2941 ggcaggactc cgctttttat tccgtagtcg gcgccgccgg cgagcttttt tcagttcgca
     3001 gcgcgcttta cgaaccgttg ccccaccata tcattctgga tgacttcgtc ctttccctga
     3061 atgtggcgaa aaagggttac cgggtcgtgt acgaaccggg cgcctgcgca acggaactgc
     3121 cgtctttatc gatggctgac gaacaccgcc ggaaagtccg catcgcagcc ggcggcttcc
     3181 aggccatttc cctgcttacc cccctcctgg ccttctggcg gcgtccgcgg ctctcttatc
     3241 tgtatatttc ccaccggttc atgcgatggg cagtcagccc gctctgtctc atcgtcgcct
     3301 acgtctccaa ctgcgtgctg gcgctgggaa caagcgtcgc catttaccgc tggcttctcg
     3361 gggcccaaac cgccttttac ggaatggctg cccttgctgc acgttttccc atcctcaaag
     3421 aaaagagccg gctgctgaag ctcggctctt atttcgtatt catgaatgtc tccgttgtcc
     3481 agggcttttt ccggtttgtt cgcggcaaac aggctgccgc atgggaaaaa gcgaggcggg
     3541 ttcaatccgc cgcgtgaccc tgcttttatc tataatttca ccacctgcac caccctggtg
     3601 acattattga ccattacttt cactacatag ctccccgacg gcaggcttgc catggggaca
     3661 ctgatcgtgt tcggtccggc gcccaccgcc tgttccatca gcgttatc
//
LOCUS       DATGKE010000098         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_183145, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000098 DATGKE010000000
VERSION     DATGKE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183145"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="VKU83_01770"
     CDS             complement(<1..2126)
                     /locus_tag="VKU83_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92301.1"
                     /translation="MFRNYFKIAWRGLLKDRQFSLLNILGLSAGICCALLIWLWVADE
                     LSVDKFFPNDSRIYEVMETNRVDGQEQITDESSGLVSDFIVAQCPEVEYAASLAPPSW
                     WPKYTLSVGDKNLKATGQYAGKDYFNIFSFPMLEGNTAKVLTDNNMIVISDELAMRLF
                     GRTDHLIGQPVRFQQQQTFFVSGVFKKPPRNSSQQFDFVLSFDYLYKQQNWVKSWNNS
                     GPHNFILVRQGTDINVLNKKITALITKASGDTSRRPVAKRFSSLYLENTFSHGVSTGS
                     KIVYVKLFSLIAIFIIVIACINFMNLSTAKAARRMKEVGIKKVVGARRPQLILQFLTE
                     SFLITLLATIVALGMCIVFLPAFNNLTGKEIILRPDKQLMEMLFGIVVITAIVAGSYP
                     ALYLSKFNPLTVLKGKLPSSFAELISRKGLVTFQYTLSATLIVSVLVIYRQVRFIQSA
                     DPGYSKENIVKFRVEGRVQQQPTAFVNAVKRIPGVVNAAFTTHNMVGRNYGTYGVNWE
                     GRDPNKGIYFEGFLCSYDFIETMGMHMTAGRSFSRSFGADTSKIIVNETAVATMHFKD
                     PIGKNIEVNGTRMQIIGVVRDFHFESLHEPVKPAFFVLMGDGVSWANIMIRIKPGQQQ
                     ETIAGIRDLYNAFNPGFPFTYDFLDEQYQKQYDTEVRVATLAKWFSGLAILISCLGLF
                     GLAAFTAQRRQKEIGIRKVVGASANQ"
     gene            complement(2257..>2927)
                     /locus_tag="VKU83_01775"
     CDS             complement(2257..>2927)
                     /locus_tag="VKU83_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLI92302.1"
                     /translation="TRITEYSTVMSPPTKEGLEVKMDLTALFHIKPEAAPAIYATVGT
                     DYGKEIVVNNFMAIVREYTMTYSAVELLGERETIEQHIEDKLRAAVAPYGIILDDVLV
                     KDIDMPAEVLRAIEDKAKADQVAKLTKLELQTKREQEDFDLESREKELKFALDKQKND
                     SLIMQIDANSIKNYQNTIGSSLTDRLLKYKSIEVTKELVKSPNAKIIITDGKTMMVNN
                     VEEK"
BASE COUNT          697 a          777 c          715 g          738 t
ORIGIN      
        1 atttggttcg cagaagcccc aaccaccttg cgaatgccaa tctccttctg ccggcgctgt
       61 gcggtgaatg ccgccagacc aaaaagaccc agacaggaaa ttaaaatagc aaggcccgaa
      121 aaccattttg ccaacgtggc gacccgcacc tccgtgtcat attgcttttg gtattgctcg
      181 tccagaaaat cgtaggtaaa gggaaaaccg gggttgaaag cgttgtagag gtcgcggatg
      241 cccgcaatcg tctcctgctg ctgaccgggt ttgatccgga tcatgatgtt tgcccaggaa
      301 acgccgtcgc ccatcagaac aaaaaatgct ggtttaaccg gctcgtgcag ggactcgaaa
      361 tgaaaatccc ttaccacgcc aatgatttgc atgcgggtac cattgacctc aatattctta
      421 ccgatcgggt ccttgaaatg catcgtcgcc acggccgtct cattcacgat gatcttgctg
      481 gtatctgccc cgaagctcct cgaaaaactt cttcctgccg tcatgtgcat gcccatcgtc
      541 tcgataaagt catagctgca caaaaaaccc tcgaaataga tgcccttatt tggatctctc
      601 ccttcccaat tgacgccgta ggtaccataa ttgcgaccga ccatattgtg cgtggtaaaa
      661 gcagcattca caaccccggg gattcgcttg acggcgttaa cgaaagccgt cggctgttgc
      721 tgcacccgcc cctcaaccct gaatttgacg atattctctt tgctgtaccc ggggtctgcg
      781 gactggataa accgtacttg gcgataaata accaacacgg aaacgatgag ggtagccgac
      841 aaggtgtatt ggaaggtgac caggcccttt cgggaaatga gttcagcgaa ggagctaggc
      901 agcttgccct tcaatacagt aagcgggttg aatttcgaca ggtacaaggc ggggtagctg
      961 cctgcaacga ttgccgtgat gaccactata ccgaacagca tttccatcaa ctgcttatcc
     1021 gggcgcagga tgatctcttt gcctgtcaga ttgttgaagg cgggcaaaaa gacgatgcac
     1081 atgcccagcg ccacaattgt cgccaggagc gtaataagaa aggattcggt caggaattga
     1141 aggatcaatt ggggccgacg ggcgccgacc acttttttta tgcccacttc tttcatccgc
     1201 cgcgcggcct tcgccgtcga aaggttcata aagttgatgc aggcaatgac gataatgaaa
     1261 atggcaatga gcgaaaagag ctttacgtat acgatcttgc tgccggtact gacgccatgg
     1321 ctaaacgtat tctctaaata caggcttgag aatcttttgg cgacaggacg gcggctcgtg
     1381 tcgccgctcg ctttcgtgat taatgcggtg atctttttat tcaatacatt gatgtctgtc
     1441 ccttgccgca ccaaaatgaa gttgtgcggt cccgaattat tccagctctt cacccaattt
     1501 tgctgcttgt agaggtagtc gaaggacagt acaaagtcaa actgctggga tgaattgcgg
     1561 ggcggcttct taaagacgcc ggacacaaag aaagtctgtt gctgctgaaa gcggaccggc
     1621 tggccaatca ggtgatcggt ccggccaaaa agccgcatcg ccagctcgtc ggaaatgaca
     1681 atcatattgt tgtctgtcag tactttcgct gtattgcctt ccagcatcgg gaaggagaaa
     1741 atgttgaaat agtccttccc ggcatattgg ccggtagctt tcagattctt gtcgccgacg
     1801 gaaagggtgt attttggcca ccagctgggc ggcgccaacg aagcggcgta ttcgacctcg
     1861 ggacactggg cgacaatgaa gtcgctgacc aggccggaag actcatcagt gatttgttcc
     1921 tgaccatcca cccggttggt ttccatcacc tcatagatcc ggctgtcatt cggaaaaaac
     1981 ttgtccacgc ttagctcatc ggcgacccaa agccagatca gcagcgcaca acagataccg
     2041 gcggaaagcc caaggatgtt gagcagtgaa aattggcggt ctttcaggag gccccgccag
     2101 gcgatcttaa aatagttccg aaacatgatg gtgaattttt cccagaaccg ttcagccaag
     2161 atggtgccat ttgcgtaacc cgttgtaaac gcataattta agcgctgaat tctgctgaaa
     2221 accgtccggt tccgtacacc ggctgttcgc ttcacgctat ttttcctcga cattattcac
     2281 catcatggtc ttaccatccg taatgatgat cttcgcattc ggcgatttca ccagctcctt
     2341 tgtgacctcg atgctcttat atttcaaaag ccggtcggtc agcgaggaac cgatcgtatt
     2401 ttgatagttc ttaatagaat tggcgtcgat ctgcatgatc aacgaatcat tcttttgttt
     2461 atcgagcgcg aactttaatt ccttttccct cgattccagg tcaaaatcct cctgttcccg
     2521 cttcgtttgc aattccagct tggtcagctt cgcgacctgg tcggctttgg ctttgtcttc
     2581 tatcgcccgc aggacctcgg ccggcatgtc gatgtccttg acaagcacgt catccaggat
     2641 aattccataa ggagcaacgg ccgctctcaa tttgtcctcg atatgctgtt cgatcgtttc
     2701 ccgctcacca agtaattcca ctgccgaata tgtcatggta tattcccgca cgatggccat
     2761 aaaattgttg acgacaatct ctttcccata atccgttccc acggtggcat agatcgccgg
     2821 cgccgcctcc ggcttgatat ggaacaatgc cgtcaggtcc atcttgacct caagcccttc
     2881 tttcgtcggc gggctcatga cggttgagta ctctgtaatc cgggtgc
//
LOCUS       DATGKE010000099         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1010997, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000099 DATGKE010000000
VERSION     DATGKE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010997"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /gene="galA"
                     /locus_tag="VKU83_01780"
     CDS             complement(<1..2159)
                     /gene="galA"
                     /locus_tag="VKU83_01780"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HLI92303.1"
                     /translation="MRIIRLNRILLALISLFATLLTAKGQLPVGRIRMNFDEGWKFHL
                     GDATDPHRDFNYSIANILAKTGDAGNTCIRMDFDDRSWSAVRLPHDWAVSLPFAHVSN
                     EDVESHGYHAVGALFPENSIGWYRKTFTLPHGDSGRRFVLRFDGIFRDSKVWINNCYL
                     GGHFSGYGGAAYDITDFIRFGGKNTVVVRADASQDEGWFYEGAGIYRHAWLESFPNVH
                     AAELGGVFVHTRLTGDQAAVTIETRVANEETHAADAAVSVYVTDREGNKLAQSSEQPL
                     SLAVGKDGLVTQTLTVAHPRLWSLDDPYLYRAVALIRSGHGNLDSVKLRFGIRTITID
                     KDRGLFLNGSPVKVHGVCCHQDHAGVGSALPDYLQYYRIQLLREMGVNAYRTSHNAPT
                     PELLDACDSLGMLVLDETRLLNSGPEYGGEFERQILRDRNHPSVFMWSIGNEEWMIQE
                     SDLGKRIAQTQILRQQELDPTRTCTYAANVGNEWTGVNQVIPVRGFNYNLDGLDAYRA
                     AHPDQPVIGTEVASTVSTRGIYIKDTVNAYVPDYDSVAPPWASTAEYWWKIAAARQWF
                     MGGFAWTGFDYRGEPTPFSWPDINSHFGIMDICGFPKTVYYYYQSWWTDKDVLHIAPN
                     WNYKGLPGQPVPVWVNTNAADVELFLNGKSLGKKPMPRNGHLEWLVNYAPGKLEAVAY
                     KNGRKLTAVVETTGEPYAVSIHPSKRRLADNGADAIV"
BASE COUNT          528 a          718 c          622 g          508 t
ORIGIN      
        1 aagacgatgg catccgcccc gttatccgcc aaccgccgtt tcgatggatg aatgctgacc
       61 gcatacggct cgccggtagt ctcgacaacc gcggtcagct ttctgccatt cttataggca
      121 accgcttcca gcttgcccgg agcataattc accaaccatt ccaaatgccc attacggggc
      181 attggctttt tccccaggct ttttccgttc aaaaagagct ccacgtccgc ggcgttggtg
      241 ttcacccaga cagggaccgg ctgccccggc aagcccttat aattccaatt gggggcgata
      301 tgcaggacat ccttatcggt ccaccacgac tgataatagt agtacacggt tttaggaaac
      361 ccgcagatat ccatgatgcc gaaatgtgag ttgatatccg gccagctgaa gggcgtcggc
      421 tcaccccggt agtcgaatcc ggtccaggca aagccgccca tgaaccattg gcgcgccgcc
      481 gcaatcttcc accaatactc cgccgtgctc gcccacggcg gagccacgct gtcgtagtcc
      541 ggcacatacg cattgacggt atcctttatg tagatgccac gggtcgatac cgtactggca
      601 acctccgtgc cgattacagg ctgatcgggg tgcgccgccc ggtaggcatc gaggccatcg
      661 aggttatagt tgaacccccg taccgggatg acctggttga cgccagtcca ttcatttcca
      721 acatttgcgg catacgtaca agttctggtc ggatcgagct cctgctggcg caggatctgg
      781 gtttgggcaa tccgcttgcc caagtccgat tcctggatca tccattcttc attgccgatg
      841 gaccacatga agacgctggg atggttacga tcgcgcagga tttggcgctc gaactcgccg
      901 ccatattccg ggccgctgtt aagcagccgc gtctcatcca gcacaagcat cccgaggctg
      961 tcacatgcgt cgagcaattc gggcgtcggt gcattatggc tggtccggta cgcgtttacg
     1021 cccatttccc tgagcagctg tatccggtaa tattggagat agtcgggcaa tgcgctgcca
     1081 acgcccgcat ggtcctggtg acaacagacc ccgtgcacct tgacagggct gccattgagg
     1141 aataaccccc tgtctttgtc gatagtgatc gtccggatac cgaacctcag cttcacactg
     1201 tcgagattac cgtgacccga ccggatgagc gctacagccc ggtacagata aggatcatcc
     1261 agtgaccaaa gccgcggatg cgccaccgtc agcgtctggg tgaccaggcc atccttgccg
     1321 accgcaaggg ataagggctg ttccgaactt tgggccagct tgtttccctc ccggtcggtc
     1381 acatagacgg aaaccgccgc gtctgctgcg tgagtctcct cattcgcaac ccgcgtctca
     1441 atggttacag cggcctgatc ccccgttaac cgggtgtgca caaagacccc gcccaattcc
     1501 gcagcatgca cgttcggaaa gctctcgagc catgcatgac gatagatccc cgccccttca
     1561 tagaaccacc cttcatcctg gctggcatcc gcgcgcacga caacggtatt cttgccgcca
     1621 aagcggatga aatccgtgat gtcataagcg gccccgccgt acccgctgaa atggccgcca
     1681 aggtaacaat tgttgatcca caccttgctg tcccggaaaa ttccgtcgaa ccgcaaaacg
     1741 aaacgccttc ccgaatcgcc atggggcagc gtgaacgttt tccggtacca gccgatgctg
     1801 ttctcgggaa acaacgcacc gacggcatga tatccgtgcg actcgacgtc ttcattgctc
     1861 acgtgcgcaa agggaagcga aaccgcccag tcatgcggca gccgaactgc cgaccaggag
     1921 cggtcatcga aatccatccg gatgcaggtg ttacccgcgt caccggtctt ggcaagaata
     1981 tttgcgatcg aataattgaa atcgcgatgc ggatccgtgg catcgcccag gtggaatttc
     2041 cagccctcgt caaaattcat gcgaatgcgg cccacaggca attggccttt tgccgtcaga
     2101 agcgtcgcga aaagggaaat aagagcgagg agaattcgat tcaggcgaat gatacgcata
     2161 tcgggctttt catcaaaaat agcccgaaaa ggcgcttacc cgataccggc gactaaaatt
     2221 taattgtctc aggcaggtac ttcgccacga ggtccgcata atatggcttg agcttttgcc
     2281 aatccggcgg cgtgggattc ttcgagtaca ggtcataagg gttgaataat ttcacccaac
     2341 ggaacatttc ccggtcgtgc tcgtccatca aatgat
//
LOCUS       DATGKE010000100         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1348130, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000100 DATGKE010000000
VERSION     DATGKE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348130"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VKU83_01785"
     CDS             complement(<1..1352)
                     /locus_tag="VKU83_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92304.1"
                     /translation="MQFIKTLCSRNIIFGAALFLLPCRAFSQSSFLPQGSRFDPFLDR
                     MQILQQTNSTLNFSSDRPFSRKTAVEMAEMADSLNTRYPYDEFYHLSAVDRATLRSLL
                     LNNMEWTRMSRDSFRSKTPWFNTFYTEKANFYQVTDKDFFLTVDPVIQETQSVESGYN
                     QRPFLNAKGLTLRGTIAGRLGFSAYITDNQERGPAFFGQRAEQFRAVPGAGYYKHFKT
                     TGYDYFDNRASIFFNAWKYFDFQIGYDKNFIGDGYRSLFLSDYAAPYLFGKINTRIWK
                     LNYQVIYMQLVDQHFAPDSDFLYPKKYGVTHHLSINAFPWMTFGIFENVIFTRTNGYE
                     LSYLNPVAFLVSAQQQNGSPDKTTAGVDLKLNIGHTVQLYGQLLVDEFILHQILHYSD
                     GYWANKQGLQAGIKYINAFSIRNLDLQVETNLVRPYTYQHDDTVDNYSHYNQPLAHPL
                     G"
     gene            1383..2723
                     /locus_tag="VKU83_01790"
     CDS             1383..2723
                     /locus_tag="VKU83_01790"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLI92305.1"
                     /translation="MRNYLITNVHLVNEGRISVGDLLVKNGRIEKIGASLRPAFAVTE
                     INGEGRYLLPGVIDDQVHFREPGLTHKATIYTEAKAAVAGGVTSFMEMPNTIPPAFTQ
                     DLLEEKYAIAARSSLANYSFYMGTSNDNADEALRTNQKKKDVCGLKIFMGSSTGNLLV
                     DNYLTLDRLFRDSEVLIATHCEDEKIIRRNLERLRQEGRELVAADHPRIRDEEACFES
                     SLTAIQFAQRHNSRLHVFHLSTEKEMQLFGNLLPQREKRITAEVCVHHLHFTSDDYER
                     LGNKIKCNPAIKAPHNREALWSALLDDRLDLIATDHAPHTLAEKTGPYEKAHAGLPLV
                     QHSLLLMLHYVKAGRISLEKLVEKMCHSPAVCFQLAERGYLREGYYADLVMIDFDLPS
                     SVRPENILYKCGWSPLEGFHFPATVTHTFVNGHLVYGNGSFDESQWGRRLRFDR"
     gene            2733..>3088
                     /locus_tag="VKU83_01795"
     CDS             2733..>3088
                     /locus_tag="VKU83_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92306.1"
                     /translation="MEIDYTTYEDLSLFSRHEEFSIFHKMDFTRTTGGKDLLVEYFNH
                     PLTSVEDIYSIQRTLKIILERLDQWPSSISNGTIMVIERFYETALDEIPHGMNLPAAL
                     GYKLFHSSDYSMVRYSV"
BASE COUNT          721 a          819 c          860 g          688 t
ORIGIN      
        1 ccgagcggat gggcgagagg ctgattgtaa tgactgtaat tatcgaccgt atcgtcgtgt
       61 tgatacgtgt acgggcgaac caggttcgtt tccacctgca ggtccaggtt cctgatggag
      121 aacgcattga tatatttgat ccccgcctgg aggccctgtt tgttcgccca gtagccgtcc
      181 gaataatgca ggatctgatg caggatgaat tcatcgacga gcaactgacc atacaattgc
      241 acggtatggc cgatattcag cttcagatcg acccccgcgg tcgtcttgtc cgggcttccg
      301 ttctgctgct gggccgagac caggaaggca acggggttca ggtaggacag ctcgtacccg
      361 ttggtacggg taaagatgac gttctcgaag atgccaaagg tcatccacgg aaaggcattg
      421 atgctgagat gatgcgtgac accgtatttt ttcgggtaga ggaaatccga atcgggcgcg
      481 aaatgctggt ctaccagctg catgtaaatg acctggtaat tcagtttcca gatccgcgta
      541 ttgatcttgc cgaagaggta tggcgccgcg tagtcactca ggaaaaggct ccggtagccg
      601 tcgccgataa agttcttgtc ataaccgatc tggaaatcga aatatttcca ggcattgaaa
      661 aaaatggaag cccggttatc gaagtagtcg taaccggtcg tcttgaaatg tttgtaataa
      721 cccgctcccg gtaccgcccg gaattgctcc gcccgctggc cgaagaacgc gggaccccgc
      781 tcctggttgt ccgtaatata tgcggagaag cccagcctgc cggcgatcgt acccctcagc
      841 gtaaggcctt ttgcgttcag gaacggccgt tggttgtagc cggactcgac cgattgtgtt
      901 tcctggatca ccgggtcgac ggtcaggaag aagtctttgt cggtcacctg gtagaaattg
      961 gccttctccg tataaaaagt attgaaccac ggcgtcttgc tccggaagct gtcgcgggac
     1021 atccgcgtcc attccatatt gttgagcagc aaagaccgga gcgtcgcccg gtcgacggcc
     1081 gataggtgat aaaactcgtc ataaggatat ctcgtgttca gcgagtccgc catctctgcc
     1141 atttcaacgg ctgtcttgcg gctgaacggc cggtctgaac tgaagttgag cgtggaattc
     1201 gtctgttgaa gaatctgcat gcggtccaga aacggatcga aacgggaacc ctggggaagg
     1261 aaactggatt gtgaaaaggc ccggcaggga aggagaaaga gcgcggcccc gaaaataatg
     1321 ttccggctac aaagggtttt aataaattgc atgataaagg ttttgcacga tcaattttct
     1381 ctatgcgtaa ttatttgatc actaacgtcc atctggtcaa cgaaggtagg attagtgtcg
     1441 gagatttgct agtcaaaaac ggccggatcg aaaaaatcgg cgcaagtctc cggcccgcat
     1501 ttgcggttac ggagatcaac ggcgaagggc gctatttatt gcccggcgtg atcgacgacc
     1561 aggtgcattt cagggaaccg ggcctcacgc ataaagcgac gatctatacg gaagcgaaag
     1621 ccgccgtggc cggcggcgtc acttctttca tggagatgcc caatacgatc ccgcccgcat
     1681 tcacgcaaga cctgttggag gagaaatatg cgatcgccgc gcggagctcg ctcgccaact
     1741 actcctttta catggggact tccaacgata atgccgacga ggcgctgcgc acgaatcaga
     1801 aaaagaagga cgtatgcggg ctcaagatct tcatgggatc gtccaccggc aacctgctgg
     1861 tcgacaatta cctcaccctc gaccggctct ttcgggacag cgaagtgctg attgccacgc
     1921 actgcgagga tgagaagatc atccggcgta acctggagcg gctgcggcag gaagggcggg
     1981 agctggtcgc cgcggatcat ccgcgaatcc gggatgagga ggcctgtttt gagtcttccc
     2041 tgacagccat ccagtttgcc cagcgacaca atagccggct gcacgttttt catctctcga
     2101 cagaaaagga aatgcagctg ttcggcaatt tacttccgca gcgggaaaaa cggatcaccg
     2161 cggaggtctg cgtccaccat ctgcatttta cttccgacga ctatgagcgg ctgggaaata
     2221 agatcaagtg caatcccgcc atcaaggccc cgcataaccg ggaggccctt tggagcgcct
     2281 tgctggatga ccggctggat cttatcgcga ccgatcatgc gccgcatacc ctcgcggaga
     2341 agaccggacc gtacgaaaaa gcgcatgcgg gtttgccgct ggtgcaacat tccttgctgc
     2401 tgatgctgca ctatgtcaaa gcgggccgga tcagcctgga aaaactggtg gagaaaatgt
     2461 gccattcacc ggccgtctgt tttcagctcg ccgaacgggg atacctccgc gaaggctact
     2521 acgcggacct ggtcatgatc gactttgacc tgccatcgtc tgtccggccc gagaacatcc
     2581 tctacaagtg cggctggagc ccgctggaag gattccactt tcccgcgacg gtgacccata
     2641 ctttcgtgaa tggccatctc gtgtatggaa acggcagctt tgatgagtct caatggggcc
     2701 ggcggcttcg gttcgaccgc taaacaacac atatggaaat cgactatact acttacgagg
     2761 acctgtccct tttcagccgc cacgaagaat tctccatttt tcacaagatg gattttaccc
     2821 ggacgaccgg cggaaaagac ctgctggtcg aatatttcaa tcatcccctc accagcgtgg
     2881 aagacatcta ttccatccag cggacgctga agatcatcct ggaaaggctg gaccaatggc
     2941 cttcttccat cagtaatgga acgatcatgg tcatcgagcg tttctatgag accgccctcg
     3001 atgagatccc tcatgggatg aacctgccgg ccgcgcttgg ctacaagctg ttccattcca
     3061 gcgactattc gatggtccgc tattcggt
//
LOCUS       DATGKE010000101         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_401539, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000101 DATGKE010000000
VERSION     DATGKE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401539"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VKU83_01800"
     CDS             <1..414
                     /locus_tag="VKU83_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92307.1"
                     /translation="REEEMKNKLYGRAFLPILVVFLVFTGAILFGAPVLKGWNTDGKV
                     LLVANELLFALTAVSYLLHLKSLRNQNPHFFVRMVYGSLVVKMLACLLAVFLYGVFAS
                     PVNKGAIFGSFILYVIYTFLEVKTLIKFLKKPGHA"
     gene            407..1027
                     /locus_tag="VKU83_01805"
     CDS             407..1027
                     /locus_tag="VKU83_01805"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HLI92308.1"
                     /translation="MPKREAPLDYLRRWIPEAAAALALDYLNRYHVHLTITRERKSIL
                     GDYRHATSSSHHRISINGNLNHYSFLITLIHELAHLETFMEHGNRVQSHGREWKKIYR
                     KILEQFLPLSVFPADVLSALRKNLHDLPASSCADENLMRVLKRYDDDEGGMVLVEQVP
                     EGGCFTLEDQRVFRKGKKLRKRYQCVEVATGKLYLFSPIYEVKPAS"
     gene            complement(1232..2014)
                     /locus_tag="VKU83_01810"
     CDS             complement(1232..2014)
                     /locus_tag="VKU83_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLI92309.1"
                     /translation="MKKLLPCVLAAGLLPALLYAGGPARQEFYAIRIYQLQTAQQEAR
                     VDSFLQQAFLPALHRHHISGIGVFKPVGNDTAAVRRIYVFIPLKSLDEYAGLLPGLAG
                     DRQYQSGGQSYLTAAYDNPPYVRMETILLQAFPDMPQHAAPWAAQGYSADRIYELRSY
                     EGPTEQYFANKVQMFNKGGEIALFEKLGFHAVFYASVVSGSHMPNLMYMTSFENKAAR
                     EDHWKAFGNDPTWKSLSSSPEYQHNVSHIDDIFLHATPYSEI"
BASE COUNT          555 a          530 c          623 g          558 t
ORIGIN      
        1 agggaagaag aaatgaagaa taagctatac ggcagggctt ttctgccgat tctggtcgtt
       61 ttcctggttt ttaccggcgc gatcctgttc ggcgcgccgg tcttgaaagg gtggaatacc
      121 gacggcaaag tcctgctcgt ggccaacgag ttgctgtttg ccctgaccgc agtgtcttac
      181 ttattacatc tcaagtcatt aaggaaccag aatccgcatt ttttcgtaag aatggtctac
      241 ggcagcttgg ttgtcaagat gttagcctgc ctgttggcgg tattcctgta cggagtcttt
      301 gccagtccgg tgaataaagg ggccatcttc ggcagcttta ttctctatgt gatctatact
      361 ttcctggagg tcaagacatt aatcaaattt ctcaagaagc cgggacatgc ctaaaagaga
      421 agcgccgttg gactatctgc gccgatggat ccccgaagct gcggcggcgc tggcacttga
      481 ctacctgaac cggtaccacg tacatttaac gattacgaga gagcgcaaaa gcatactggg
      541 ggactaccgg catgcgacgt catccagcca tcaccggata agtattaacg gaaacctgaa
      601 tcattactcc ttcctgatca cgctgatcca cgagctcgct cacttggaaa ccttcatgga
      661 gcacgggaac cgggttcagt cgcacggcag ggaatggaag aagatctacc gcaagatcct
      721 ggagcaattc cttccgctgt ccgtttttcc cgcggatgta ttgtcggcgt tgagaaaaaa
      781 tttgcatgat ctccctgcca gcagttgtgc ggatgaaaac ctcatgcggg tgttgaagcg
      841 atacgatgac gacgagggcg gaatggtact ggttgagcaa gtccccgagg gcggctgttt
      901 taccctcgag gatcaacggg tattcaggaa agggaaaaaa ctgagaaaaa ggtatcaatg
      961 tgtcgaggta gcgacaggaa agttgtatct tttcagtccg atttacgaag taaagcctgc
     1021 ttcttagccc gtttttaatc ctttcccctg ttctatctcc ccatttcctc tcagctcaat
     1081 aaggccataa ggctgtacag tacggcgacg gtaacaataa tgatcccaat gaagggtcga
     1141 tctgcgtgtt tcatagtcga tttggcgaga atgatcattc ggtaaacgcc aaagcaactg
     1201 gaaatagtat gtttcgggtg gctgttcttt gtcagatttc cgaataaggc gtcgcatgca
     1261 ggaaaatgtc gtcaatatgg gacacattat gctggtattc aggcgaagag gaaagtgatt
     1321 tccaggtagg atcattaccg aaggccttcc aatggtcctc gcgcgccgct ttgttttcaa
     1381 aggaggtcat gtacatcagg tttggcatat gcgaaccgga gacgaccgac gcatagaaaa
     1441 cagcatggaa tccaagcttt tcgaaaagcg cgatttcacc gcctttgttg aacatctgca
     1501 ctttgttagc aaaatattgc tccgtagggc cttcgtaact gcgtagctcg tagatcctgt
     1561 cggcgctata cccttgtgca gcccatgggg ctgcatgttg cggcatgtcc gggaaggctt
     1621 gcagcaggat ggtttccatc ctgacgtaag gcggattatc gtacgctgcg gtcagatacg
     1681 actgtccgcc gctttgatat tgacggtcgc ccgccagccc gggcaataaa ccggcgtatt
     1741 cgtcgaggct tttcagggga atgaagacat aaatacggcg gacggcggcg gtatcgttcc
     1801 ctacgggttt gaatacgccg atgccgctga tatggtgacg atggagggcc ggaaggaaag
     1861 cctgctggag aaagctgtcg acccgggcct cctgctgggc cgtttgaagc tggtaaatgc
     1921 ggatagcata gaattcctga cgggccgggc cgccggcgta caatagggcg ggaagcaggc
     1981 cggccgcgag gacgcacggg agtaactttt tcatgattaa acggttcaat aagcggtaaa
     2041 tgtaaatcgt aaaatcattt taacaaaaac ttgaagcagt cccgggagcg gttttgggaa
     2101 atagggctat cttggcgcac tcctgaatga ctatgcgata tatttttttt tcagtaattc
     2161 tgctgctggc cgggggaatc agcgccgagg cgcagcagtt cctgaccgga aaggtctatg
     2221 taaagggaac cgccgataca ctgatctccg tcagtatcca caatat
//
LOCUS       DATGKE010000102         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1217047, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000102 DATGKE010000000
VERSION     DATGKE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217047"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VKU83_01815"
     CDS             complement(<1..1043)
                     /locus_tag="VKU83_01815"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HLI92310.1"
                     /translation="MIISGFKKAMARGRNWYARYERPFSSLSLIGGFIFDAVTLRRVD
                     MFWENFWVVAHLFIVGACIILVDRQDLEIGAEANPEKTHFWLVNVMQFFFGGILSTYL
                     VFYFRSGALSTSWPFLLLLVLAFWANESFKREYVRLSFQIVLFYLSLFAFTIYLVPVL
                     VHAIGPWIFVVSGLASLVLMAGFLALLNYFTHYKFSKSKKVIILSIGLTFLAVNGLYA
                     LNIIPPIPLSLKDAGVYHAIEKDPQGDYVVYSEKRGWFSFLDLFEDFHASSTDNVYVY
                     SAIFSPPLLDIKIVHHYEYYDAKNERWIDEGKITLPVLGGRESGFRTYSMRSGLMDGK
                     WRVNVETENGQVIG"
     gene            complement(1093..1524)
                     /locus_tag="VKU83_01820"
     CDS             complement(1093..1524)
                     /locus_tag="VKU83_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92311.1"
                     /translation="MNKTVTVIIGLVLIAGSFYGGMKYDQSQAAAARSARIGQFAGQF
                     GSAGGRGAGFAGARGGGSFITGQILSKDSQSITIQLTSTSTPSAGSAIVFISASTTIS
                     KTSMVPLSDLAVGQNVIVSGQKNPDGSVSANLIQLRQTNGQ"
     gene            complement(1696..>2161)
                     /locus_tag="VKU83_01825"
     CDS             complement(1696..>2161)
                     /locus_tag="VKU83_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92312.1"
                     /translation="ISDPTAADFSVLNQNDIISSASSISNTLTYLLAAVAGISLIVGG
                     IGIMNMMLTTVTERTREIGLRKAIGAKKGDISRQFLIEAVVLTVVGGVIGIALGWGIS
                     FLLNATGILATSVSWYSVILAFGVSAVIGIIFGYYPAKRAAGLNPIDALRYE"
BASE COUNT          609 a          542 c          520 g          490 t
ORIGIN      
        1 ccgatgacct ggccgttctc ggtttcaacg tttacccgcc atttgccgtc cataaggcca
       61 ctcctcatcg aataggtgcg aaagccgctt tcccttccgc ccaagacggg cagtgttatt
      121 ttgccttcgt caatccagcg ctcatttttc gcatcatagt attcatagtg atgcacgatc
      181 ttaatatcaa gcagaggcgg cgaaaaaatg gcgctataga catagacgtt gtcggtcgag
      241 ctggcgtgaa aatcctcaaa aagatcgaga aaggagaacc agccccgctt ttcagagtac
      301 acaacgtagt ctccttgcgg atctttttct atagcgtggt agacgccggc gtctttgagc
      361 gagagcggta tgggcggaat gatgttcagc gcatagaggc cattcacggc aaggaatgtc
      421 agtccgatcg agagaatgat gaccttttta cttttagaaa atttgtaatg ggtaaaataa
      481 ttcaggaggg cgagaaatcc cgccataaga acaagactgg ccaatcctga aacgacgaaa
      541 atccacgggc cgattgcgtg caccaacacc ggaacgagat aaatagtgaa agcaaaaagc
      601 gaaagataga acaggacgat ctgaaaactc aagcgaacat attccctttt gaaagattca
      661 ttggcccaaa aggcgaggac gagaaggaga agaaacggcc agcttgtcga aagcgcgccg
      721 ctcctgaaat aaaatacaag ataggtggaa agaatgcctc cgaaaaagaa ttgcatgaca
      781 ttcaccagcc aaaagtgggt cttttcggga ttggcttcgg caccgatctc taagtcctgg
      841 cgatcgacga ggatgatgca cgcgccgaca atgaaaaggt gggcgacgac ccagaaattt
      901 tcccaaaaca tgtcaacccg gcggagcgtc actgcgtcaa aaatgaaccc gccgatcaaa
      961 gacagggatg agaacggacg ttcatagcgc gcgtaccaat tccggccgcg agccattgct
     1021 tttttgaagc cggagatgat catgcattaa agatattcct tatcttccat gatgtccata
     1081 caaacaaatc ctttactggc cgttcgtttg gcggagctgt atcaaattgg cgctcacact
     1141 gccatctgga tttttctggc cgctcacgat gacattttgt ccaacagcca ggtcagacaa
     1201 tggaaccatg ctcgttttgc ttatggtcgt cgaagcggaa atgaaaacaa tagccgaacc
     1261 tgctgacggg gtcgatgtgc tcgtaagttg gatagtgatg ctttgggaat ctttactcaa
     1321 gatctgtccg gtaataaagc ttcctccgcc tcgggcgccg gcaaaccctg cgccacgtcc
     1381 acctgcgcta ccgaactgcc cggcaaactg acctatccga gcgctgcgtg cggcagccgc
     1441 ttggctttgg tcatatttca taccgccgta aaacgaacct gcgatcaaaa ccaaaccgat
     1501 aataacagtt actgttttat tcatattatt tctttattat ttaattttta aaccctgcag
     1561 acctcgaact ttcaaattcg gatgcattgc taggcgatcg aagaatttga ttggaagcgt
     1621 actggtcgta cgatgagaat caaattaccg aagctcaaca acgcagggcg ccgaatttga
     1681 aagttcaacc cttgatcatt cgtatcgtaa ggcatcgatg gggttcagcc ccgccgcccg
     1741 cttcgccgga tagtatccga aaatgatacc gatcacagct gatacgccga aagctaaaat
     1801 aaccgagtac cacgaaacgc tggtggccaa tatacccgtc gcattcaaga ggaatgaaat
     1861 gccccaccct aaagcgatgc cgataacacc gccaacgaca gtcaaaacta ccgcctcgat
     1921 caagaattgc cggctaatat cgcccttctt cgccccgatg gctttacgca gaccgatctc
     1981 ccgagtgcgc tcggtcaccg tggtaagcat catgttcatg atgccaatgc cgccgacaat
     2041 aagcgagata ccggctacgg cggccaaaag ataggtaagg gtattcgata tagatgaagc
     2101 actggaaatg atatcgttct ggttcagtac gctaaagtca gctgctgtcg gatcgctgat
     2161 a
//
LOCUS       DATGKE010000103         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_96263, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000103 DATGKE010000000
VERSION     DATGKE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96263"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VKU83_01830"
     CDS             complement(<1..864)
                     /locus_tag="VKU83_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92313.1"
                     /translation="MYLCKTYFSFNYGTWSTKELVAAGAGAGATVLVLANINSTCDAW
                     DFVLYCREAGLRPVLGAEIRNGNKLLYILLSANNAGFRGINAFLSEHLLEKKEFPDTP
                     AGAFAGPQDGFIIYPFGSRSPEALGDNERIGVLPDEVNRLLRTGFAATASKWVIRQPV
                     TYQNKVYYNLHRLLRAVDKNTLLSRLQPEDMAGPREYFVPAAEVLAAFQRYPAMIANT
                     SRLLEACSIDMEFGADKNKRLFTASREDDRSLLEKLASDGLRLRYGKNEKARQQVVRE
                     LQIIDQMGFTAY"
     gene            989..1282
                     /locus_tag="VKU83_01835"
     CDS             989..1282
                     /locus_tag="VKU83_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92314.1"
                     /translation="MIRPNRDHRYELIKPMIETGRITFFNDIFKYIPKTVVAKDLGKK
                     VDRFNGLMSRVEQFMLHDLFIIAGFFDIDEDVMMRLVMNDYKRAKEKGLHLEG"
     gene            1279..>2551
                     /locus_tag="VKU83_01840"
     CDS             1279..>2551
                     /locus_tag="VKU83_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HLI92315.1"
                     /translation="MKRTTWILTLVLAIEGGMAQPLYHERWRPQFHFSPKAHWMNDPN
                     GMVYYKGVYQLFFQYYPDSSVWGPMHWGYAESRDLVHWQEDPVALFPDKLGYIFSGSA
                     VVDKSNTSRFGQPDDVPLVAIFTQHDPVRAAQNRNDFQNQSIAYSLDNGQDWLKYDHN
                     PVLKNPGVSDFRDPKVFWYPEGHQWVMVLAVGDHVGFYTSPNLKDWYQAGEFGKDAGA
                     HGGVWECPDLFPLNDGRGHLQWVLVVNINPGGPNGGSATQYFLGQFDGRTFTPSTAGA
                     RWLDYGPDEYAGVTWSNTGNRRIFLGWMSNWLYGQRVPTSPWRSANTLPRELGLERVG
                     DSLYVKSTPVRELGELVTRTERTKLSAQLPALAKLHVRTKTIGSFDLVLSNAAGDSVV
                     IGYDSAAARYHIDRRHAGVAMGPDFDRDYTAP"
BASE COUNT          558 a          689 c          776 g          528 t
ORIGIN      
        1 ataggcggtg aaccccatct gatcgatgat ctgcagctcc ctgacgacct gctgacgcgc
       61 cttctcattt ttgccgtagc gaagccgcag tccgtcgctg gccagtttct ccaggaggct
      121 gcggtcgtct tcccggctcg cggtgaacag ccgcttattc ttgtcggcgc caaattccat
      181 gtcgatcgaa caggcctcga gaaggcggga ggtattggcg atcatggccg gatagcgctg
      241 aaaggccgca agcacttcgg cggcggggac gaaatattcc cgcgggccgg ccatatcttc
      301 cggctgaagc cgggacagga gcgtattctt atcgactgcc cgcaacaggc ggtgaagatt
      361 ataatagacc ttattctggt acgtgaccgg ttggcggatc acccactttg aggccgtggc
      421 cgcgaaaccg gtccgcagaa ggcggttgac ctcatcggga aggacgccga tccgttcgtt
      481 gtcgccaagc gcttcgggag accgcgaccc gaaggggtaa ataatgaagc catcctgcgg
      541 tccggcaaag gctcctgccg gcgtatccgg aaactccttt ttttccagca ggtgttcgga
      601 gagaaaggcg ttgatgccgc gaaagcctgc gttatttgcc gacaacagga tatataacag
      661 tttattgccg ttccggatct ccgcgcccag aaccgggcgc aggcccgctt cgcggcaata
      721 caggacaaag tcccaggcgt cacaggtgct gttgatattc gccaggacca gcaccgtcgc
      781 gccggccccg gcgcccgcgg ccaccagctc ctttgtcgac caggtgccgt aattgaagct
      841 aaagtatgtc ttgcacaggt acatgggtta ttttagtaac aagaatgcta aattaattag
      901 taaatttgta cggctcaccg ttcctgaggg aattaaacgg agcaagaaaa taagttttac
      961 ggactaacac ataaaacggt ttttagccat gataagacct aacagggacc atcgttatga
     1021 actgatcaaa cccatgatcg agaccggccg gatcaccttc ttcaacgata ttttcaagta
     1081 cattccgaag acagtcgttg ccaaagacct tggaaagaaa gtcgaccggt tcaacggcct
     1141 gatgagcagg gtggagcaat tcatgctgca cgatcttttt atcatcgccg gttttttcga
     1201 catcgacgag gatgtcatga tgcggttggt catgaacgac tataagcggg cgaaggaaaa
     1261 aggattacat ttggaaggat gaagaggaca acctggatcc tgacgctcgt cctggccatc
     1321 gaaggcggaa tggcgcagcc gctgtatcat gaacggtggc ggccgcagtt ccatttttcg
     1381 ccgaaggccc attggatgaa cgaccccaac gggatggtat attataaggg cgtttatcag
     1441 ctgttctttc agtactatcc ggacagcagc gtctggggcc cgatgcattg gggctatgcg
     1501 gagagcaggg atctcgtgca ttggcaggag gacccggtcg cgctgttccc cgacaagctg
     1561 ggctatatct tttcggggag cgcggtggtg gataagagca acacttcccg cttcgggcag
     1621 ccggacgatg tgccgctggt ggcgatcttt acgcagcatg acccggtcag ggcagctcag
     1681 aacaggaacg attttcagaa ccagagcatc gcctacagcc tggacaatgg gcaggactgg
     1741 ttgaaatacg atcacaaccc ggtgctgaag aatcccggcg tcagcgattt tcgcgatccc
     1801 aaggtattct ggtatccgga agggcaccaa tgggtcatgg tgctggcggt cggggaccat
     1861 gtggggttct acacctcgcc gaacctcaag gactggtatc aggccggcga attcggcaaa
     1921 gacgcggggg cacacggcgg ggtctgggaa tgcccggatc ttttcccgct caacgacggc
     1981 cgcgggcacc tgcagtgggt cctggtcgtg aacatcaacc ccggcggtcc caacggcggg
     2041 tcggctaccc aatattttct cggtcagttt gacggcagga cctttacccc ttccacggca
     2101 ggcgcgcggt ggctggatta cggacccgac gagtatgcgg gggtcacgtg gtcgaatacg
     2161 ggtaaccggc ggatcttcct cggctggatg agcaattggc tttatggcca gcgggtgccc
     2221 acatcgccgt ggcgaagcgc gaataccctg ccccgggagc tgggactgga aagggttggc
     2281 gactcattgt atgtaaagtc cacgccggtc cgggaactgg gggaactggt aacccggacg
     2341 gagcgaacca aattgtcggc gcagctcccg gcgctcgcga aattgcatgt caggacaaag
     2401 accatcgggt cctttgacct cgtgttgtcg aatgccgccg gggacagcgt tgtgatcggc
     2461 tatgactcag cggccgcgcg ataccatatc gaccggcggc atgcgggcgt cgcaatgggg
     2521 ccggattttg accgcgacta tacggcgccc c
//
LOCUS       DATGKE010000104         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_805425, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000104 DATGKE010000000
VERSION     DATGKE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805425"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..924)
                     /locus_tag="VKU83_01845"
     CDS             complement(76..924)
                     /locus_tag="VKU83_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HLI92316.1"
                     /translation="MKNSVHYIFSIAAGLVLLASCKKDEAKVYFEGGTAPVLTSSATD
                     SISLPVNDTTATAATFTWTNPNYMFSDGPSSLNVHYYLQFDTTSAFSSANMQTVGINS
                     SLSVTYTVAQLNAILANGLLLSTGNPHTVYIRVQSFIQPFTSTSQPEGTLSSSPLNFM
                     VTPYAPPPAVTPPANDSLYIVGSAVPDNWANPIPSADLASETFTMVSPTEYKLTTTLT
                     GGGEYKLISVNGSWTNQWSVATADTYPNGGPFVSNGNNSIAPAATGTYLIDVNFQTGK
                     FTVTAQ"
     gene            complement(948..2531)
                     /locus_tag="VKU83_01850"
     CDS             complement(948..2531)
                     /locus_tag="VKU83_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI92317.1"
                     /translation="MKRSFLTIASLAAIIAFGLVSCSKDLNREPPNSITASQAFSTPQ
                     GAKEALAKVYGAYALTGSNGSGSSDLGGIDAGTSDFVRLMWDANELSTDEAVCAWNDP
                     GVPDFHNLGWNSGNTILLGLYARCVYQITVANSFIHQAASLKFTGQDATNLGHYVAEA
                     RFLRAFQYWELMDLFGNPPFVTDKDPIGAGSFLPKQISRADLFTYVESELKSLDSAGA
                     LVAPMQNEYARADQACVWALLARMYLNAEVYTGTPRWNDAITYASKVIGAGYALMPHY
                     QDLFLADNNVGNTEQILSIAYDGSNTQNYGGTTFIINAEIGGSMVPANYGVPGGGWGG
                     NRTTANIPKLFPNTSGNIDTRGLFYTAGQSEQINTIGAFTDGYPSTKFQNVTSAGQTP
                     AGASVYASTDFPLFRLAEQYLIYAEAVLRGGTNGTMPQAVQYFNALRERAYGNTNGDV
                     VSISLQDILGERGRELTWEGFRRTDLIRFGDFTSQSYLWPWKGGVSSGTGVDAHYNIF
                     PIPATDLNANTNLKQNPGY"
     gene            complement(2566..>4723)
                     /locus_tag="VKU83_01855"
     CDS             complement(2566..>4723)
                     /locus_tag="VKU83_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLI92318.1"
                     /translation="VNSQVAANPADSTFSDKMGAASTDWQKLIFQTAITNNDNLSVSG
                     TAGNLPYRISVGYHNEQGILKTDQFERGTVGIHLTPHLLDDHLKIELNLNGALTKSRF
                     ANQGAIGAAVAFDPTQSPYQKGSPYGGYFEWQEAGNLNPLATRNPLALLYQDNNIGYA
                     ANSVGNLHLDYALPMVPGLHAIANLAYDVTDGHGTNTVPGNAAQQWGLTPNHGLMSKY
                     KQNNTYVLQEYSLNYNKDLPSIKSNINVLGLWSYANTLTTPYNYPSLDSKRDTVPGTQ
                     PVFPTSPFENTLISYVGRVIYTYDQRYILTASIRDDGSSRFAPNERWGIFPAISGAWH
                     VSQEEFLKNNRVLSDLKIRASYGVTGNQDGLGDYNYIPAYSLSGNQSRYEFGNTFYNM
                     FTPSAFITNFKWEQTTSTNVGVDFGFLNNRISGSIDYYNKNISNLFNNVFIPVGSNFT
                     NQATINIGTMKDQGMEFTLNANPVRSGKFNWDLNFNFTYNKNTITKLTNGNQPGFYGD
                     QTGGIGGGTGNTIQIQTVGQEANSFYVLQQVYDKSGQPIEGAFVDNNRDGQITQLGDA
                     IHYKSPFAPVFLGFSSTFTYDKWSLNLVTRANIGNYVYNNVQAGLGSSQYIMNPLQYL
                     QNAGSSYLQTHFYSPQYFSSYYVENGSFFKLDNLGLTYNVGSIGSGASLRVSGYCQNV
                     FVITKYTGIDPEVYNGIDNNIYPRARTFTLGASLNF"
BASE COUNT          994 a         1212 c         1386 g         1131 t
ORIGIN      
        1 gagcaggccg cggcaaaggt catcaacacg ccgctcagaa gggtatattt taacgaattt
       61 ctcatattaa gatgtttatt gcgcggttac ggtaaatttt ccggtttgga aattcacatc
      121 gatgagatat gtcccggtgg cagccggcgc gatactgttg ttgccgttgg atacgaaggg
      181 tccgccgttg ggataagtgt cggcggttgc gacgctccac tgattcgtcc acgatccgtt
      241 tacgctgatc agtttgtatt cgcctccgcc ggttagcgtg gtggtcagtt tgtattccgt
      301 cgggctcacc atcgtgaatg tctcgctcgc aaggtcagcg gatgggatcg gattagccca
      361 gttatcggga accgcgctgc cgacgatgta aagggagtcg ttggctggcg gcgttactgc
      421 cggaggcggc gcatacggcg taaccataaa gttcagcgga ctcgacgaaa gcgtaccttc
      481 cggctgggat gtggaggtaa acggctggat gaaggactgc acgcggatat acaccgtatg
      541 aggatttccg gtggacaaca gcagtccgtt ggcaaggatg gcattcagct gggcaaccgt
      601 gtacgtaacg ctcaggctgg aattgatgcc gacagtctgc atgttggcgc tcgaaaacgc
      661 cgacgtcgta tcgaactgca ggtaataatg aacgttcagc gaactcgggc cgtcgctgaa
      721 catataattc ggattggtcc aggtaaaggt ggcggccgtg gctgtcgtat cattcacggg
      781 cagtgaaatc gaatcggttg ccgaggacgt cagtaccggc gccgtccctc cttcgaaata
      841 aactttcgcc tcgtctttct tgcaggacgc cagcagcacc aaccctgcgg ctatactgaa
      901 tatataatgg actgaatttt tcatatcgct actttaactt ttgttattta gtacccgggg
      961 ttttgtttca aattcgtgtt ggcattgagg tcggtcgcgg ggatcgggaa aatattatag
     1021 tgcgcatcca ctccggtacc ggatgagacg ccccctttcc acggccacag gtacgactgg
     1081 gacgtgaagt cgccgaaccg gataaggtcc gtgcggcgaa acccttccca agtcaattcc
     1141 cggcctcttt caccgaggat atcctgtaga ctgatgctga cgacgtcgcc attggtattg
     1201 ccataagccc gttcgcgcag cgcattgaaa tattgcacgg cctggggcat cgtcccattg
     1261 gtaccgcccc tgaggactgc ttccgcatag atcaggtatt gctccgcaag ccggaagagg
     1321 ggaaagtctg tgctggcata aacgctggct cccgcgggcg tctggccggc cgaggtcacg
     1381 ttctggaact tggtagatgg gtatccgtcg gtgaaagcgc cgatcgtgtt gatctgttcg
     1441 gattggccgg cggtgtagaa cagcccccgg gtgtcgatgt tcccgctggt gttggggaag
     1501 agcttgggaa tattggccgt cgtacggttg ccaccccagc cgcccccggg aacgccgtag
     1561 ttggcgggaa ccatggagcc gccgatctcg gcgttgatga tgaaggtcgt accgccatag
     1621 ttctgggtat tgctgccatc gtaagcgatc gagaggattt gttcggtatt gccgacattg
     1681 ttgtccgcca ggaaaaggtc ctggtaatgg ggcatcagcg cgtatcctgc gccgatgacc
     1741 ttggacgcat aggtaatcgc atcgttccag cggggggtgc ccgtatacac ttcggcattc
     1801 agatacatcc tggccagcag ggcccacacg caggcctggt ctgcacgggc atattcattc
     1861 tgcatgggcg ccaccagggc tccggcactg tcgaggctct tgagctcgga ctcgacatag
     1921 gtgaacaggt ccgcccggga gatctgcttc ggtaaaaagc tgccggcgcc gatcggatcc
     1981 ttgtcggtga caaacggcgg attgccgaac aggtccatca gctcccaata ctgaaaggcg
     2041 cggaggaacc tggcttctgc aacataatgg cccaaattgg tggcgtcctg gcccgtgaac
     2101 tttagacttg cggcctggtg aataaaagaa ttagccaccg tgatctggta cacgcaacgg
     2161 gcatagaggc ccagcagaat cgtattgccc gagttccagc ccaaattgtg gaaatcagga
     2221 acgccggggt cattccaggc gcagaccgct tcatccgtgc ttagctcatt ggcgtcccac
     2281 atcaggcgga cgaagtccga agtacccgcg tcgatgcccc caaggtcaga actgcccgac
     2341 ccattgctgc ccgtcagggc ataggcgccg tagaccttgg ccagcgcctc tttcgccccc
     2401 tgtggcgtgc tgaacgcctg tgacgcggtg atgctgttcg gcggctcccg gttgaggtct
     2461 tttgaacagg agacgaggcc aaaggcgatt atggctgcca ggcttgctat cgtcagaaaa
     2521 gaacgtttca tattcaaatg ctttataaga caataattca acatattaga aattgaggct
     2581 tgcaccgagc gtgaaagttc tcgcgcgcgg ataaatattg ttgtcaatcc cattatagac
     2641 ctcggggtcg atgcccgtgt atttggtgat cacaaacacg ttctggcaat acccgctcac
     2701 tcgcaggctg gcgccggatc caatgctacc aacgttgtaa gtcaggccca ggttgtccaa
     2761 cttaaagaaa gatccgtttt cgacgtaata gctggaaaaa tactgtggcg aatagaaatg
     2821 ggtctgcaaa tagctgcttc cggcattctg caggtattgc aggggattca tgatatattg
     2881 gctgctgccg aggcccgcct gcacgttatt atagacgtag ttcccgatat tggccctggt
     2941 gaccaggttc aggctccact tatcgtaggt gaacgtgctg ctgaaaccca agaacaccgg
     3001 cgcgaacggc gatttgtaat ggatggcatc gcccaattgg gtgatctggc cgtcccggtt
     3061 attgtcgaca aaggcgcctt cgatcggctg cccgctcttg tcatagacct gctgcagcac
     3121 atagaaggag ttggcctcct ggcccacggt ttggatctgg atcgtgttgc cggtgccgcc
     3181 gccgatcccg ccggtctggt cgccgtaaaa gccgggctgg ttaccgttcg tcagcttcgt
     3241 gatcgtattc ttattgtagg taaagttgaa attcaggtcc caattgaatt taccggaacg
     3301 gactgggttg gcgttcaacg taaattccat accctggtct ttcatcgtgc cgatattgat
     3361 ggtcgcctgg ttggtgaaat tcgagcctac agggatgaat acgttgttga acaaatttga
     3421 gatgttctta ttataatagt cgatgctacc gctgatccgg ttgttgagga atccgaagtc
     3481 gacaccgacg ttggtcgacg tcgtctgctc ccatttgaag ttcgtgatga aggccgacgg
     3541 cgtaaacatg ttatagaaag tattgccgaa ttcgtaccgg gattggttgc cgcttaagct
     3601 gtaggctgga atataattgt agtccccaag cccgtcctgg ttacccgtaa cgccgtagct
     3661 ggccctgatt ttcaggtcag acaacaccct gttatttttc aggaattcct cctggctgac
     3721 gtgccaggcg ccggatatgg ccgggaaaat cccccaccgt tcattgggtg caaagcggga
     3781 ggaaccgtca tcccggatag aggcggtgag gatatatcgc tgatcgtacg tgtagatcac
     3841 ccggccgaca taggagatca gggtattctc gaatggggaa gtcggaaaga caggctgggt
     3901 acccggtacc gtatcccttt tcgaatcaag cgacgggtaa ttgtaaggcg tcgtcagggt
     3961 gttcgcataa ctccaaagcc cgagtacgtt gatattactc ttaatggacg gaaggtcctt
     4021 gttgtaattc aggctatact cctgcagcac atacgtattg ttttgcttgt atttgctcat
     4081 caggccgtgg ttcggggtga gtccccactg ttgcgcggcg ttacccggca ccgtattcgt
     4141 cccgtggccg tccgtcacgt cgtacgccag gttggcgata gcatgcagac ccggcaccat
     4201 gggcagtgcg taatcaagat gcaggttgcc gacgctgttg gcagcatacc cgatattgtt
     4261 gtcctggtac aggagcgcca ggggattgcg ggtagccagc gggttcaggt tgccggcctc
     4321 ctgccactcg aagtaccctc cataagggct tcctttttga taaggagatt gggtcggatc
     4381 gaaagcgacc gctgcgccga tggcgccctg attggcaaaa cgggattttg tgagggcgcc
     4441 gttcagattc aattcgatct tcaggtgatc gtcgagcaaa tggggtgtca ggtggatccc
     4501 aaccgtgccc cgctcgaact ggtccgtctt caggattccc tgttcgttgt gatacccgac
     4561 ggagatccga tagggcagat taccggcggt ccctgaaaca ctgaggttat cgttgttggt
     4621 gatagcggtc tggaagatca gcttttgcca gtcggtgctg gccgcgccca tcttgtcgga
     4681 gaacgtcgaa tcggcggggt tggccgccac ctgggagttc aca
//
LOCUS       DATGKE010000105         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_699212, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000105 DATGKE010000000
VERSION     DATGKE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699212"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VKU83_01860"
     CDS             <1..758
                     /locus_tag="VKU83_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92319.1"
                     /translation="MHKIRWFLLPVAIWTGIIVAANLAHMLPTGWQGPSYVLGAVAGL
                     LPLAGDITRSLKQGKVDLGVPVLITILILLYARQFLITDIFVLLIVAGDIFKEYILWR
                     VEQSVREISHALPDIAFRKTDELREVRIENIIIGDVLVVKAGGRVPVDGVLQGDEALL
                     DESVITGGSRLITKEKGAHLVAGSINQGSIFEMKVLSASENSTLSQIHWMVEEAQRHS
                     SPLSRFTDRFALINALAADKIRYIRDFKPRASG"
     gene            761..1105
                     /locus_tag="VKU83_01865"
     CDS             761..1105
                     /locus_tag="VKU83_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92320.1"
                     /translation="MIGDGINDAPAISEANVGIAMGLKGTDITLNSAKVVLMNDNIGA
                     LPYTLTASRKVLKIIRLNLYLATTIHLVAAGLSAAAILSLLGSTLMHQVSSALVLLIR
                     LDCIPYVRPLHD"
     gene            complement(1446..1589)
                     /locus_tag="VKU83_01870"
     CDS             complement(1446..1589)
                     /locus_tag="VKU83_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92321.1"
                     /translation="MQLAAIDERSAAARYRETQAVGLPQIGLSYTAMTTDNPLNAFGF
                     KLQ"
     gene            complement(1697..2002)
                     /locus_tag="VKU83_01875"
     CDS             complement(1697..2002)
                     /locus_tag="VKU83_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLI92322.1"
                     /translation="MNVFNDLFGKTGDLKTVYLKGATILDVRSSEEFRTGHIKGAVNI
                     PLGSLNESIQDLQKAGKPVIACCKSGVRSRLAHIVLSRAGLEAYNGGPWETLESVIR"
     gene            2133..>2597
                     /locus_tag="VKU83_01880"
     CDS             2133..>2597
                     /locus_tag="VKU83_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLI92323.1"
                     /translation="MADEQQIKALFPFFEPELIAGVTEKATIRRYAAGQLLMRTGQNI
                     RSTLVITEGLVKIYREDEEGGEFFMYYLQPGQACALSMICATKQETSGIMARAMTDTE
                     AISIPLEFMDKWMTQYKSWYHFVLESYRSRFEELLVTVDHIAFRHMDERLLFY"
BASE COUNT          584 a          642 c          729 g          642 t
ORIGIN      
        1 acatgcataa aatacgttgg ttcctactgc ctgttgcgat atggacaggg attatcgtcg
       61 cggccaatct ggcccatatg ctgccaacgg gttggcaggg gccttcctat gtactcggtg
      121 cggttgcggg cttgctcccg ctggcgggag atattacacg gtcgctgaaa cagggaaaag
      181 ttgatttggg tgttccggtt ctaataacca tccttatttt actgtacgca cggcagttcc
      241 tgatcaccga catctttgtg ctgctcattg tagcgggcga tatcttcaag gaatatatcc
      301 tgtggcgggt cgagcaatcc gtcagggaga tctcccatgc cctgcccgac atcgctttcc
      361 gcaagacgga cgagctgcgc gaggttcgaa ttgagaatat catcataggg gacgtgctcg
      421 tggtgaaggc agggggcaga gtgcctgtcg acggagtgct gcagggcgat gaagcattac
      481 tggacgaatc ggtcataacg ggcggatcga gactgatcac aaaagaaaaa ggggcgcacc
      541 tggtggccgg gtccatcaat cagggcagca tattcgagat gaaggtcctc agcgcgagtg
      601 aaaactccac cttgtcgcag atccactgga tggtggaaga agcccagcgc cactcttcgc
      661 ccctgagcag gttcacggac aggtttgcgc ttatcaatgc cttggctgcg gacaagatcc
      721 ggtacatccg ggatttcaag cccagggcga gcgggtgatc atgatcggcg acgggatcaa
      781 tgacgctccc gccatttcgg aggcgaacgt cggaattgcg atggggttaa aaggaacgga
      841 tataacgttg aattccgcga aagtcgtatt gatgaacgat aacattggtg cgcttcctta
      901 caccttaaca gcgagcagga aagtactaaa gatcatccgg ctgaaccttt atctggcgac
      961 aaccattcac cttgtggcgg cggggctgtc cgcggctgcc attctatcgc tgctgggcag
     1021 cacgttgatg catcaggtca gcagcgcgct ggtgctgcta atacgcttag attgtattcc
     1081 ttacgttagg cctttgcacg attaagattt taatttcgct tgttggcgcc gggccgtatc
     1141 ttgggaacag gcttgcaaga tgcgcaaaaa tatgccccct cctggagagg agggaggcaa
     1201 tacactatta ataattcgtt ccggtgtaaa ggtaggatgc tgtttacaaa tccggtctac
     1261 caacaatttt tttaacagtc tgcaattcaa gattaaaatt tttcatgcct gtcgggtttt
     1321 cgcgctggaa gagctggtcc aggttgagaa tgggctgctt tacatccagg cttgtcaggg
     1381 aagtccggca cggctttagg gtggttaagc aggtcagggg caaagtcggt ctggccgatc
     1441 gatgcctact gcagcttgaa cccaaatgca ttcaacggat tgtcggtcgt cattgccgta
     1501 taggagagcc caatctgcgg cagacctaca gcctgcgttt cccgatacct ggctgcggct
     1561 gacctttcgt cgatggctgc gagctgcaag tcgcggttgt tggcgatatc attccactta
     1621 atattctgcc aaattatttt gacggcatga tcctttccgt gacattggtc acgtgacggg
     1681 agaacgtcgt tacctcttac cggataacgc tttccagtgt ctcccagggt ccgccgttgt
     1741 acgcttcgag gcctgctctg gaaaggacga tgtgcgccaa tctgctcctg acgccgcttt
     1801 tgcagcacgc gatgaccggt ttgcctgctt tctgcagatc ctgaatgctt tcgttcagac
     1861 tgccgagcgg gatattgacc gctcctttga tatggcccgt cctgaattct tcggacgagc
     1921 ggacgtccag aatagtggca cctttgaggt agacggtctt cagatcgccg gtttttccaa
     1981 aaaggtcatt gaatacgttc atgtttttga atttttgaat ctttcttttg cgttgacgag
     2041 acaaaaatac tgcggctgaa aagtgccggc cgtgactttt atcacgtgga cggccgcttc
     2101 tccctacatt tgtccggcgt aaggcaatcg ttatggcaga tgaacagcaa ataaaggcgt
     2161 tatttccctt cttcgaaccg gaattgattg ccggcgtgac ggagaaagcc accatccggc
     2221 ggtatgcggc gggccaactg ctcatgagaa ccggccagaa catccggtcg accctcgtca
     2281 taaccgaagg actggtcaag atctacaggg aggacgagga aggaggcgaa ttcttcatgt
     2341 attatctgca gcccggacag gcctgcgcat tgtcgatgat ctgcgccacg aaacaggaga
     2401 cgagcgggat catggcgcgg gccatgacgg atacggaagc tatcagcatc ccgctcgaat
     2461 tcatggacaa atggatgacc cagtataaga gctggtatca tttcgtgctg gaatcttacc
     2521 gttcccggtt cgaagagttg ctggtcacgg tcgaccacat cgcctttcgc catatggatg
     2581 aaaggctact cttctat
//
LOCUS       DATGKE010000106         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1007978, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000106 DATGKE010000000
VERSION     DATGKE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007978"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="VKU83_01885"
     CDS             complement(<1..1855)
                     /locus_tag="VKU83_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLI92324.1"
                     /translation="MKMIYSVLSILVPTFLAMVPARGSNYYFSSMNGDDSRSAGQAQH
                     PATPWRSLLKLNQIFPQLRPGDSVLFERGGVYYGTIEAGASGTDGRPIVFAAYGRGYD
                     PIISGFSRLKGWKAGGSNGVLQAPCPECKARVNMVTVGNEPVQMGRYPNTGYLTVQQH
                     NGNTSITDNSLSGGADWTGAEAVIRKNRFIIDRNTIISQQGGTLTLKGGSVYYQPTDN
                     FGYFIQNDIRTLDQDGEWYYDPKAHAMNLYYSSGSVPGDVDASSVDTLVVISGKQNLV
                     FTGLTVEGSNGNAFFLTNASNITISNCRILFTSQDAVLALKSNDLVLDHLYIDHSNDD
                     GVCFLGGSGSRIIDCTIKRTGTVPGMGNFERSYDGIYLEGENTTVEYNKVDTSGYVGI
                     LFHGGPDSIKNNSVDYFCFVKDDGGGIYTWSGDIDSATTRHTGYVIGNIVLNGITATS
                     GTDSRQQGIANGIYLDENTGMIEVTGNTVAHCTIGIFVQDTHEAVVKDNTLYDNGSQI
                     ALRHALAKGTLRNNEVAGNTAVAAKKDQTVLTLTSGVSGEVSSFTKMHDNHYYEPGGG
                     SFYNTSVRQNNRSVQEKGAIGDWQSKYGKDANSVELAPAGAVKFEYNAGKNV"
     gene            2134..3501
                     /locus_tag="VKU83_01890"
     CDS             2134..3501
                     /locus_tag="VKU83_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HLI92325.1"
                     /translation="MRLRPREIHLLILSVDLFILNASFFLVRLFLDEGDPECIKQLLI
                     LLNIGYVIISPIFVENLRDLKSDYPKMARSLVRRFVYYFAFTALILLFTDQTACPKRQ
                     YLSTISLFFLLKFCISSWFFFRYFFNTHLYLKPTIIIGDNKLGSQLQDYFLTNKYLGI
                     KPIGLLADRPASQHSHNVIGSVTDFQKIFDARPFEDAFIVLPLSEVEKIKYLIAVAER
                     NGVRTHLVPNFAGSQEIHFKVGSLGDIPLLQMRTFPLDTYANRFLKRAFDIVFAATMM
                     VCLVPLGILIAIAIKLTSRGPVFFKTHRLGVTGAPFTIYKFRTMVHCPDADNPLKSTV
                     EGDRRITRVGRFLRKYSLDELPQLLNVLTNDMSIVGPRPHRLNLNKTLQQKMGPYMMR
                     HTIKPGITGWAQVNGWRGPTETKLQYWGRTLHDLWYIEHWNFWLDLYIILLTLVGKKT
                     RKNAF"
BASE COUNT          822 a         1057 c          880 g          955 t
ORIGIN      
        1 ttacgttttt gcccgcatta tattcaaact ttacggcccc cgcgggcgcc agctcgaccg
       61 aattcgcatc cttgccgtat tttgactgcc agtcgccgat cgcgcccttt tcctgaacgc
      121 ttcggttgtt ctgccgtacc gaggtgttgt aaaaggaacc tccgcccggc tcataataat
      181 gattgtcatg catcttggta aaggaactga cctctccgga gacacccgag gtcagtgtaa
      241 ggacggtctg gtccttttta gccgcaacag ccgtgttgcc ggccacttcg ttgttccgga
      301 gcgtgccttt ggccaaagca tgccgcagcg cgatctgcga accattgtcg tagagtgtgt
      361 tgtccttcac caccgcctcg tgtgtatcct ggacaaagat cccgatggta cagtgcgcca
      421 ctgtgttgcc ggtcacttcg atcatgccgg tgttttcatc gaggtagatc ccgttggcga
      481 tcccttgctg gcgggaatcg gttccgcttg tagccgtgat gccgttcagt acgatgtttc
      541 caatgacata cccggtatgg cgcgtcgttg ccgagtcgat atcccctgac caggtgtata
      601 tgccgccgcc gtcgtcctta acgaagcaga agtagtccac tgaattgttt ttgatcgaat
      661 cgggaccgcc gtggaataaa atacccacat aaccggaagt gtccactttg ttgtattcca
      721 cggtcgtgtt ctccccctcc aggtagatgc cgtcatagga tcgctcgaaa tttcccatcc
      781 cgggcacggt gcccgtacgt tttatggtgc aatcgatgat ccggctcccc gacccgccga
      841 ggaaacatac cccatcgtcg ttcgagtggt cgatataaag gtggtcgagc acgaggtcgt
      901 tcgacttcag cgccaggacg gcatcctggg aagtaaagag gatgcggcag ttcgagatcg
      961 tgatgttcga ggcattcgtg agaaaaaaag cattcccgtt gctgccttcg accgtcagcc
     1021 cggtaaaaac gaggttctgt tttccgctga tgacaaccag cgtgtccaca ctcgatgcgt
     1081 cgacgtctcc gggcaccgat cccgaactat agtagaggtt catcgcatgg gccttggggt
     1141 cataatacca ctcgccatcc tggtcaaggg tccggatatc gttctgaatg aaatacccga
     1201 aattgtccgt cggctgataa tataccgagc cgccctttag cgtgagcgtc ccgccctgtt
     1261 gcgaaatgat cgtgttgcgg tcgataatga accggttttt cctgatcacc gcctccgccc
     1321 cggtccagtc ggcgccgcca gacagactgt tatcggttat cgaagtatta ccgttatgtt
     1381 gctggacggt cagatacccg gtgttcgggt agcggcccat ctgtaccggt tcattcccca
     1441 ccgtcaccat atttacccgc gccttgcatt caggacatgg cgcttgcagt acaccgttac
     1501 tgccgcctgc tttccatccc ttcagccggc tgaaaccgct gattatagga tcataacccc
     1561 gaccataagc cgcaaaaacg atcggtcggc catcagtccc cgacgcgcct gcctcaatcg
     1621 tgccgtaata caccccgccc cgttcgaaca atacggagtc cccgggccgc aattgcggaa
     1681 agatctggtt tagtttaagc aggctccgcc agggagtcgc tggatgttgg gcctgccccg
     1741 ctgacctcga atcgtcgcca ttcatcgaag agaaataata attgcttccg cgtgcgggaa
     1801 ccattgccag aaaagttggc accagtattg ataaaacaga gtagatcatt ttcatgttcg
     1861 tcctttaagt attaaaactt agttgtttgt aaaaaaacca cctgaaaatg caatttaatc
     1921 cctaactttg cccctggaaa gcccgcgaat tgatcttacc ggacagaaaa atcctttctt
     1981 aagaaaccac cacctcgagc gctgccagaa atcctgtttc tgtttacaaa cgccgttcca
     2041 ttattatctg ttaagaacgc cattatcaac cccactccca aattggaccg aaggaacttt
     2101 tgctgagtcg tatgtaatct taaatgtcac taaatgagat tgagacccag ggaaatccac
     2161 ctgttgatcc tttccgttga tctgtttatc ctaaacgcat cattttttct cgttaggtta
     2221 tttctcgacg aaggggatcc ggagtgtatc aaacaattat tgatcctgct gaatatcggc
     2281 tacgtgatca tcagcccgat ctttgtggag aatcttcggg atctgaagtc cgactacccc
     2341 aaaatggcgc ggtcccttgt ccggcgcttc gtgtattatt ttgcctttac tgccctcatt
     2401 ctgctcttta ccgatcagac ggcttgtccg aagcgtcagt atctcagcac catctccttg
     2461 ttcttcctgt tgaaattctg tatttcgtcg tggttttttt tccgctattt cttcaacacc
     2521 cacctctatc tgaagccgac gatcatcatt ggggataaca agctgggctc ccagttgcag
     2581 gattattttt tgactaataa atatttgggt atcaagccga tcggtctact cgccgaccgg
     2641 cccgccagcc agcatagcca taacgtgatc ggttcggtca ccgattttca gaagatcttc
     2701 gacgcccgcc cctttgaaga cgcatttatc gttttgccgc tgagtgaggt ggagaaaata
     2761 aaatacctta tcgccgtcgc cgaacggaat ggcgtccgca cccacctcgt accaaatttt
     2821 gccggcagcc aggagatcca ttttaaagtg ggcagcctgg gcgatattcc cctcctccaa
     2881 atgaggacct ttcccctgga cacttacgcc aaccgcttcc ttaagcgggc gttcgatatc
     2941 gttttcgcgg cgaccatgat ggtttgcctc gttccgctgg gaatcctgat cgccattgcc
     3001 attaagctga ccagccgggg accggtcttt ttcaaaacgc accggctcgg cgttaccggc
     3061 gcgccattca ccatctataa attccggacg atggtccact gtccggacgc cgataatccg
     3121 ctgaagtcca ctgtagaagg tgaccggcgc atcacgcggg taggacggtt tctgcggaaa
     3181 tacagcctcg acgagctccc gcagctgctc aacgtgctga cgaacgatat gtccatcgtt
     3241 ggcccccggc cgcaccggct gaatctgaac aagactttgc aacagaaaat ggggccgtat
     3301 atgatgcgcc ataccatcaa gccgggaata acgggttggg cacaggtcaa cggttggcgc
     3361 ggtcccaccg aaacgaaact gcaatattgg ggcaggaccc tccacgacct ctggtacatc
     3421 gagcattgga atttttggct cgatctttat attatcttgc tcacgcttgt cgggaaaaaa
     3481 acccgtaaaa atgccttttg agtgcgttga accccctaca gatcaatccg tactgaccct
     3541 aaattactta tttttataac atgaaaaagt tcaatggctt attcctcctg atcatgttag
     3601 gtaccgcctg ggtcatgctt ttttgctcct cctgcacaaa cgccaagaag cttaccatca
     3661 tgcaggacct cagtccgaac cagacccttc cctccccccg gcccgctccc attt
//
LOCUS       DATGKE010000107         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_388956, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000107 DATGKE010000000
VERSION     DATGKE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388956"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VKU83_01895"
     CDS             <1..479
                     /locus_tag="VKU83_01895"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLI92326.1"
                     /translation="LEDLFLVRKLQPWASNVGKRLVRAPKVYIRDSGLTHTLLKIKDY
                     DDLLGHPAVGGSWEGFVIENLLADLPTWASPYFYRTSAGAEIDLVIEAGNKKVIAIEI
                     KRSILPSLSKGFLIGCQDVNATHRYFVYSGVERFKISTGVTALPLTMMIDELKTLW"
     gene            682..1992
                     /locus_tag="VKU83_01900"
     CDS             682..1992
                     /locus_tag="VKU83_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HLI92327.1"
                     /translation="MKLPLYPILAVASLLAALLFFAGCAAEKPVDAPAIVSVMDSLLD
                     QKEYFRLERQLTLHLPDLSKARRLYYAAILDNAFNRDSDCARRVDSLMGAERGGLSDS
                     LKAKLLRYQGASYFKTGLYRAAAGVDSLLLHDYKTVMDSSTYADIKNDSLIRNALRDV
                     PPQQVSAGTGSEVSWSRDALALIELPIRLHAQSVNAIFDTRANISSITRTFAEKLRLR
                     LLNVRYDEGAGMTGIQFKVGLAVADSLWIGNVLVRNAIFQVMPDSIFYIAPIHFQLNL
                     ILGYPIIAGLQEVRLFGDGKMEIPSTAEPGGAHNMFMDRLDPVLRLTSGADTLLFDFD
                     SGAGTTVLYSTYFRLHQAAVQAKGTKKKTSLGGAGGVKTSDTYELPALPLTLGARTVR
                     IDSVTVLTEKITPGERFSGNLGEDFMRQFKLLVINFRYVYVRAE"
     gene            2059..2508
                     /locus_tag="VKU83_01905"
     CDS             2059..2508
                     /locus_tag="VKU83_01905"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLI92328.1"
                     /translation="MNTITNEIKEFVNRTKLGFVATVCPDGSPNLSPKGTVLAWDKDH
                     LVFADIHSPGSVANLKLNPAIEINVVDIFTRRGYRFKGQAEIFTEGSVFEEVVAFLRA
                     AGSTHVIRHIVRVNVDRLQPVWSPAYDAGQTEEEIRRRWIDYWDEKR"
     gene            2824..>3109
                     /locus_tag="VKU83_01910"
     CDS             2824..>3109
                     /locus_tag="VKU83_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92329.1"
                     /translation="MGKDRFTYKEAFSALTDSKEGAIKQLLSDMTRRGLLLRLRKGMY
                     QIIPYEQDATNYLPNWHLTAAGLVKDADYYIGYYSALQIHQLITQPSLKEQ"
BASE COUNT          812 a          729 c          800 g          768 t
ORIGIN      
        1 tattggaaga tcttttttta gttcgtaaac tccaaccctg ggcctcaaac gtcggaaaaa
       61 gattggttcg ggcaccaaag gtatatattc gagatagtgg tcttacccat acgcttctaa
      121 aaataaaaga ctatgacgac ttattgggcc atccagcggt tggtggtagc tgggaagggt
      181 tcgttattga gaacctgttg gcggatcttc caacatgggc atctccctat ttttatagga
      241 cgtccgccgg ggcagaaatt gatttagtga ttgaagccgg aaataaaaaa gtgatcgcaa
      301 tagaaattaa acgatccatt ttgccatcgt taagcaaggg gtttttaatt gggtgccagg
      361 atgttaatgc aactcatcgc tactttgttt attcaggagt ggagcgtttt aaaatatcta
      421 caggagtaac tgctctccct ctcacaatga tgatcgacga attaaagaca ttgtggtaag
      481 agaacaatta aatgacaaga caaaccccaa ggggagacta atatcttgcc tggctgagct
      541 tgagcacgaa atttccgcct ctgtcggcag cttgttcctg ccaatggcaa gacgcgccgt
      601 tttgaaatgg atatacatga ttggcgggtt aaatgatgga aggttggaca atattgcctc
      661 aatttggtaa attagaccca catgaagctc ccgctctacc cgattctcgc ggtcgcatcc
      721 ctgctcgctg cgctcctctt ttttgccggc tgcgcggccg aaaaaccagt agacgcgccg
      781 gcaatcgtca gcgtaatgga ttctctgctc gatcaaaagg aatatttccg gctggagcga
      841 cagttgacgc tccacttgcc ggatctgtca aaggcaaggc gtttatatta cgcagccatc
      901 ctcgacaatg catttaaccg ggacagcgac tgcgcccgtc gggtcgattc gctgatggga
      961 gccgaacggg gaggcttatc cgattcgctg aaggcaaagt tgctacgata ccagggagca
     1021 agctatttta agacagggct ataccgggca gctgcggggg tggacagcct gctgttacac
     1081 gattacaaaa cggtcatgga cagttcgacc tacgcagaca tcaaaaacga ctcgctgatt
     1141 cgaaacgcgc ttcgggacgt cccgccgcag caagtgtcgg ccggaacggg ttcggaagtg
     1201 tcctggagcc gtgatgcact cgcactgatc gaattgccta tccgtctcca cgctcaatcc
     1261 gtcaatgcca ttttcgatac gcgggccaat atttcatcca ttacccggac gttcgccgaa
     1321 aagctgcgtt tgcgcctgct gaacgtgcgt tacgatgaag gggccgggat gaccggaatc
     1381 cagttcaagg tcggactggc cgtcgctgac agcctctgga tagggaatgt gctggtgcgc
     1441 aatgcgatct tccaggtgat gccggactcg atcttttaca tcgcgccgat tcatttccag
     1501 ttaaacctga tccttggcta tcccattatt gcgggcttgc aggaagtccg gctcttcggc
     1561 gatggcaaaa tggagattcc gtccacagct gaacccgggg gtgcgcacaa tatgttcatg
     1621 gaccggctcg acccggtgct ccggctgaca tctggcgcag acacgctcct tttcgatttc
     1681 gacagcggcg caggcactac cgtcctgtac tccacgtatt tccgcttgca ccaggcggca
     1741 gtccaggcaa aaggaacaaa aaagaaaaca agcctcggcg gtgcaggcgg tgtgaagaca
     1801 agcgacacct acgagctgcc ggcattaccg ctgaccctcg gcgcgcggac ggtccgtatt
     1861 gacagcgtaa cggtgttgac ggagaagatc accccgggcg aaagatttag cgggaatctg
     1921 ggggaggact ttatgcggca gtttaaatta ctggtgatca atttcagata cgtgtatgta
     1981 agggcggagt agcgcattgt gccgcaaggg aagttacgcc tcgactacct ttaacaagcc
     2041 cggaacaatt tcggcctgat gaataccatt acaaatgaga taaaagagtt tgtcaatcgc
     2101 acgaagttgg gcttcgtcgc aacagtctgt cctgacggta gtcccaatct ttcgccaaag
     2161 gggactgtgc tcgcttggga caaagaccat ttggtttttg cggacattca ttctcccggg
     2221 tctgttgcca atctaaagct caaccctgcc atcgagatca atgtagtcga tatttttacg
     2281 cgacggggtt atcgatttaa aggccaggca gaaattttta ccgaagggtc ggttttcgag
     2341 gaagttgtcg cgtttctaag agccgcgggt tcaacgcatg tcatccggca catcgtgaga
     2401 gtaaatgtgg atcgattaca accggtctgg tcgccggcat atgatgccgg gcaaacagaa
     2461 gaagagatca ggcgacggtg gattgactat tgggatgaaa agaggtgagc cagtatcccg
     2521 gccagtacga aagttggatt gcttccgcca taaataagaa aaccgctgat ttacagcggc
     2581 ttacttatct cgtggagctg agggggctcc aatcgaacac ctaacatcta ttttgctgtt
     2641 aatcaggcac tcaatagtta tcttatatag cttaaatcag ctttgaactg attagttcat
     2701 ataaaaattt tacgtgtagt tattatttca tagctttata gtcaggataa acctgactaa
     2761 gtggatataa aagataaata catatcaaag caatcgggcc ggcttttgtc ctattttatt
     2821 caaatgggta aagatcgctt tacctacaag gaagcttttt ccgcgttgac agattcaaaa
     2881 gaaggcgcga tcaaacaact gctaagcgat atgacacgtc ggggactgct gcttcggctg
     2941 aggaaaggaa tgtatcaaat cataccgtac gagcaggatg caactaacta tttaccgaac
     3001 tggcatctga ctgcggcagg cttggtaaaa gacgccgatt actatatagg ttattattct
     3061 gccctacaaa ttcatcaact gattacacaa ccttccctaa aagaacagg
//
LOCUS       DATGKE010000108         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_486085, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000108 DATGKE010000000
VERSION     DATGKE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486085"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..640
                     /locus_tag="VKU83_01915"
     CDS             368..640
                     /locus_tag="VKU83_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92330.1"
                     /translation="MVLTTEISVFPNPFEHFIFLEVTCDEPLDCIILLADLGPGRIVR
                     MMGAGMRDGINRLSLDNLQSLSPGQYQLDIKAVSGDLLYQTLLIKS"
     gene            671..1387
                     /locus_tag="VKU83_01920"
     CDS             671..1387
                     /locus_tag="VKU83_01920"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLI92331.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPDVVRAAMDAQRFGADGITVHP
                     RPDERHIRYKDVFDLKAIVTTEFNIEGNSQEQKFVDLVLAVRPSQVTLVPDALNQITS
                     NHGWDTIRHRTYLIDTVKRFQDAGIRVSLFVDPVLAMVEGAAETGTDRIELYTEAYAR
                     EFPANPENAIASYYAAAVRAAELGLELNAGHDLDLQNLRYFCSRIPGLKEVSIGHALI
                     CDALYLGFENAIQLYRRQLQ"
     gene            1567..2685
                     /locus_tag="VKU83_01925"
     CDS             1567..2685
                     /locus_tag="VKU83_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLI92332.1"
                     /translation="MKKGLRAGLWLAILQSAFVSFSHAQLTALPDGGNKRASVSERIG
                     ITDVTINYNRPGVKGREGKIWGQLIPVGYNDLGFGTSKAAPWRAGANENTTIEFTTDV
                     TVEGQPLPAGKYGFFIAYDPSECTLIFSRNSTSWGSFFYDQQEDALRVKVKPVPMDQE
                     VEWLKYEFTDEKENSATICLQWEKTCIPFRVEVDYIKTQLESFRRELRSDKGFTWNAW
                     VQAAQFCAQRNTDLDEGLAWADKAVSDPFVGVRNFQTLSTKAQILSLQGKTAEAAAAM
                     KEALPLGTVLEVHQYARQLIGQKRAKEALEAFQGNYDRHPNEFTTNMGLARGYSAVGD
                     YKKALDYLRKAQAQAPDKLNRDNIGKLIPILEQGKDFN"
     gene            complement(2682..3758)
                     /locus_tag="VKU83_01930"
     CDS             complement(2682..3758)
                     /locus_tag="VKU83_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLI92333.1"
                     /translation="MIFPVIGMAQKAPAPHFLVKGQIKGLEEGSDVSISAVSNPADTV
                     STGKVRNGVFTLTGHVDEPNLYEINFLGAKKKVPVFIGNDRITMAGSIDDLKTLKTTG
                     SPSNNDFTEFQDLFNPYFARLNMLMGMLHSPEGASRQDSLYRVFKGLTDSVFVMLDGF
                     INRKTNSYVSAFVLVVVNQLTDDVVAQQRRLHSLSPAVQHSFYAEYLGKQLDDAQVGA
                     VGTQEIDFTQSDTAGHAVSLSSFKGKYVLVDFWASWCEPCRMENPNVVSTYKKFKDKN
                     FTVLGVSLDKARAPWIKAINDDGLAWTQVSDLKYWYNEAAAKYHIQQIPQNLLVDPSG
                     RIVARNLRGPALEAKLCELLGCSN"
     gene            complement(3802..4404)
                     /gene="hisIE"
                     /locus_tag="VKU83_01935"
     CDS             complement(3802..4404)
                     /gene="hisIE"
                     /locus_tag="VKU83_01935"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HLI92334.1"
                     /translation="MNAEVDFEKFPDGLAPAIVQDSVTDKVLMLGFMNREALDKTRTE
                     GKVTFFSRSRKQLWTKGQESGNYLLLKGISVDCDRDSLLIKALPLGPVCHTGADTCWN
                     ERNRRDDFLVSLEEIIETRRRQASPDNSYVAGLFSQGINKIAQKVGEEAVELVIEAKD
                     HDDRLFLNEAADLLFHFLILLNAKGYNLQDVVDVLQGRHQ"
BASE COUNT         1092 a         1299 c         1057 g         1032 t
ORIGIN      
        1 aagctcgtcg gccgcttcta acaccgccgc ccccctgctc ccgcgagccc aattgttttt
       61 gcgaattttc gcaatttcga ccgctgaaac ccgcaaattt tttcccaaac ccaaagaata
      121 attcccattt ttggaatctc atttgccaag ctggaaccgc aagttattgc aaatcaaaat
      181 cttgcctttt ggcaaaatat tagccgagat taaatcaaat cacgtactat gaagaccctt
      241 atctatctcg tgaaagtttt aacccttaat accttctccc aggaaaaaat gccggcattg
      301 atcccggtac gggtggtgac gaaaattcat ccccttaaat aactgctaat tccaaatttg
      361 tagaacaatg gtcctgacca ccgagatttc ggtattcccg aatccttttg agcatttcat
      421 attcctggaa gtaacctgcg acgagcccct tgactgtatc atcctcctgg ccgatcttgg
      481 acccggcagg atcgtcagaa tgatgggcgc aggaatgagg gacggcatca accggctttc
      541 gctcgataac cttcaatccc tttcacctgg tcaataccag ttagatatca aagccgtctc
      601 cggcgacctt ctctaccaaa ctttgttgat caagagctga gccctaccct tctttgttta
      661 catttgcggc atgacgaagc tctccgtaaa cattaacaaa tttgccactt tgcgcaacag
      721 ccgtggcggc aacaatccgg acgtcgtccg ggcggcaatg gacgcccagc ggtttggcgc
      781 tgacggaatt accgtccacc cgaggcctga tgagcgccat atccgctata aggacgtttt
      841 tgatctgaag gcgatcgtca ccacagagtt caatattgaa gggaattccc aggaacagaa
      901 attcgtcgat ctcgttctgg cggtacgccc aagccaggtc accctggtgc ctgatgcgct
      961 caaccagatc acgtccaacc atggctggga taccatccgg catagaactt atctcatcga
     1021 caccgtcaag cgattccagg acgctggtat ccgggtttcc ctctttgtcg atccggtcct
     1081 cgccatggtt gaaggcgcgg ccgaaaccgg aacggaccgc atcgagcttt acacggaggc
     1141 ctatgcccgc gaattcccgg caaatcccga aaacgcaatt gcctcctatt atgcagccgc
     1201 tgtccgcgcc gccgagctgg gcctggaact caacgccgga catgacctcg atctgcagaa
     1261 cctgcgatat ttttgtagcc ggatcccggg cctgaaagaa gtgtcaatag gtcatgccct
     1321 gatctgcgat gccctttatc tcggtttcga gaacgcgatc caactttacc ggcgccagct
     1381 ccaatagttt tcaaccgtta gccttccgtt caaccgttcg caagccggtc cgccttgacc
     1441 cgctttacgg cctgatagct ttacatcctg ataataggcc tcaggtgtaa tattgtttgc
     1501 ttcggccgcc tgccatacgc tacctttaat ccgctaaccc catccatcac caaaatagtc
     1561 atcaatatga aaaagggtct gagagccggc ctttggctgg ccatcctgca gtcagcgttt
     1621 gtttccttca gtcatgccca actaaccgct ctccccgacg gcggaaacaa aagggcgtcg
     1681 gtctcagagc ggataggcat caccgatgta accatcaatt ataaccggcc cggcgtcaaa
     1741 ggccgcgaag gcaagatttg gggccagctg atcccggttg gttacaacga tctgggattc
     1801 ggtaccagca aggcggcgcc ctggagggcc ggcgccaatg aaaacaccac catcgaattc
     1861 accaccgacg taacagtcga aggacagccg ttgccggccg ggaaatacgg attctttata
     1921 gcctatgatc cttcagaatg cacgctcatt ttctcccgca attccacctc ctggggtagc
     1981 ttcttctacg accaacagga ggacgccctc cgggtaaaag tgaagcccgt accgatggat
     2041 caggaagtcg aatggctgaa gtacgagttt acggatgaaa aagagaattc tgccaccatt
     2101 tgcctccaat gggaaaagac ctgtatcccc ttccgggtag aggtcgacta tattaagaca
     2161 cagctcgagt cattccgccg cgagctccgc agcgataaag gattcacatg gaacgcctgg
     2221 gtccaggcag cacagttctg cgcccaacgc aataccgatc tcgacgaagg cctggcctgg
     2281 gctgacaagg ccgtcagcga cccctttgtc ggcgtaagga attttcaaac cctgtccacc
     2341 aaagctcaaa tcctttcgct gcaagggaaa accgctgaag ccgcagccgc catgaaagaa
     2401 gcactgcccc tgggcaccgt tctggaagtc caccaatatg cccgccagtt gatcggtcaa
     2461 aaacgggcaa aggaggccct tgaagcgttc cagggcaatt atgaccggca tccgaacgaa
     2521 ttcacgacga atatgggcct ggccagaggc tattccgccg tcggggatta caagaaagcg
     2581 ctcgattatc tgaggaaggc acaggcacag gcgcccgata aactcaacag ggataacatc
     2641 ggcaagttga tcccgatcct tgaacagggg aaagacttca attagttgct acaacccagc
     2701 aattcgcaga gcttggcttc caatgcgggc ccccggagat tgcgcgccac gatcctgcca
     2761 cttggatcga cgagcaggtt ttgggggatt tgctgaatat ggtatttggc cgccgcctcg
     2821 ttataccagt atttcaaatc gctgacctgc gtccaggcaa ggccatcgtc attgatagcc
     2881 tttatccagg gcgctctcgc tttatccagg ctcaccccaa gtacagtaaa attcttgtct
     2941 ttgaatttct tgtaggtcga aacgacattc gggttttcca tccggcacgg ctcgcaccag
     3001 cttgcccaga agtcgacgag cacatatttc cccttgaagg acgacaggga aacggcgtga
     3061 ccggccgtat cgctttgcgt aaagtctatt tcctgcgtcc ccaccgcgcc aacctgcgca
     3121 tcgtcgagct gcttcccgag atattcggca tagaaactgt gctgcaccgc cggcgacagg
     3181 gaatgaagac gccgctgctg cgccacaaca tcgtccgtga gctggttgac cacaacaagg
     3241 acaaaggcag acacgtagga attggtcttt cggttgatga acccatccag catgacgaag
     3301 acagaatctg tcagaccctt gaagacccgg tatagtgaat cctgcctgga ggcgccctca
     3361 ggagaatgca acatccccat caacatattg agccgcgcaa aatacgggtt gaacaggtcc
     3421 tggaactccg tgaaatcgtt attggatgga gaacccgttg ttttcagggt cttcaggtcg
     3481 tcgatagatc cggccatcgt gatcctgtca ttcccgatga atacgggcac cttcttttta
     3541 gccccgagaa aattgatctc ataaagattc ggctcgtcta catggcccgt cagtgtaaaa
     3601 acgccattcc ttacctttcc ggtcgacacg gtgtccgccg gattgctgac ggccgagatc
     3661 gagacatcgg aaccctcttc caaccccttt atctgccctt tgaccaggaa atgaggcgcg
     3721 ggagccttct gagccatgcc aataacgggg aagatcatta atgctgcaac aaataatttt
     3781 atacgcatga tggtttgctt tctattgatg ccgtccctgc aaaacgtcta ccacatcttg
     3841 caaattatag ccttttgcat ttaaaagaat aaggaagtgg aacaagagat ccgcagcttc
     3901 attaaggaat agccggtcat cgtgatcctt cgcttcgatt accaattcca ccgcttcctc
     3961 accgaccttt tgggctatct tattgatccc ttggctgaaa agtcccgcca catacgagtt
     4021 atccggactc gcctgccgcc gccgggtttc aatgatctcc tcgagggaaa ccaggaaatc
     4081 atcccgccga ttgcgctcat tccagcaggt gtccgcgccc gtgtgacaca ccggtcccag
     4141 cggcaaggcc ttgatcaaca gggaatcccg gtcgcagtcc acagatatcc ccttcaacag
     4201 gagatagttg ccgctctcct ggcccttggt ccacaactgc ttccgcgaac ggctaaagaa
     4261 ggtcacttta ccttcggtcc gggtcttgtc aagggcttcc cggttcataa aacccagcat
     4321 caggaccttg tccgtcacgc tgtcctgcac aatagccggt gcaagcccgt ctggaaactt
     4381 ttcaaaatca acctccgcgt tcatattata aaagatttaa attctcaccg cgatcccatg
     4441 agtccgcaaa tatcctttta attccggaat cgtgatctcc
//
LOCUS       DATGKE010000109         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1320256, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000109 DATGKE010000000
VERSION     DATGKE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320256"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1294)
                     /locus_tag="VKU83_01940"
     CDS             complement(23..1294)
                     /locus_tag="VKU83_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92335.1"
                     /translation="MRLSDTLSLAYRTIRGNRLRTGITVAIIAFGIMALVGINTAIEA
                     MKQKFTESFSAMGANGFTIHYKNWFNFHGDGVKSSKRGSLKEKRSNTNEPITKLQAET
                     FLARYNYPATVSLGISGVGDAVVSVGDQKTNPNIRVQGGDANYIDLNGFTLAAGRNLN
                     QLDIQSGRNVCVIGSAIARKFFGENPERPVEKIIHINNLPFRVVGVLASKGSTLGMNY
                     DNMILTSYNNVRRLFSTGSATSFNIQVKVPDVRQIDGAIDQAEGIFRPVRRLDVTEAD
                     NFLIDKSDTFVNLLLKNLSFITWAAVVIGVITLVGAAVGLMNIMLVAVTERTKEIGLV
                     KAIGGRQRSVRQQFLFESVIISLIGAAFGVVLGVVVGNTFSIVLSTGFVLPWIWLLLG
                     IAMCTVVGLAAGAYPAYKAGKLNPIEALRYE"
     gene            complement(1345..2742)
                     /locus_tag="VKU83_01945"
     CDS             complement(1345..2742)
                     /locus_tag="VKU83_01945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLI92336.1"
                     /translation="MNAILISAVWGIIMMFSGIFTGRKAAVRNLAIIGLVALLIGNYA
                     DLEGYHLIVMDVHKMLFFDSFGLLGNSIAFGCTLLLVLLSGKDMEKIGHNLAEYYALV
                     FFTLTGIALVTTFNSLLMLFLGIEIVSIPLYILTGSDKRNLKSNEAALKYFLLGSFST
                     GLMLLGIAFLYGASPAGTFNIDEIWTAKPAVTPLLLSGLLLLLVSIAFKVSAAPFHFW
                     TPDVYDGAPTVFTSFMATIVKVSIFVGFVRLFDEAFGTDPAQWQIFVAIIAAATLFIG
                     NITAVFQQSVKRMLAYSSIAQAGFMLLALLSLSGVAKEGIILYAAAYSIATIGVFAVL
                     IRMKDYTFEGFNGLARQQPLLAATCTIFLLSLAGIPLTGGFFAKYYMLASVVSTGRFL
                     WLVVFAVLCAAVSAYYYFRVIQAMYFKDGDGTTEAAPGAGYKGMLVVLAVLVLVLGVF
                     PQSLLGQLSRLIYTW"
     gene            complement(2739..>3367)
                     /locus_tag="VKU83_01950"
     CDS             complement(2739..>3367)
                     /locus_tag="VKU83_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLI92337.1"
                     /translation="YASLVAIRQDDLKRLVAYSSIAHVGLMCLAIFATTSIGMQGVMI
                     QMFNHGINILGMWVVVELIERKTGTRKISQLGGLARTAPGLSILLVVVALANIALPLT
                     NGFIGEFLLFTGVFTSAATQYNVLFTVVGAVTVILAAVYTLNMIKNVFFGDVNALTSR
                     VTDIRLNEKLALAVIVVLIVFIGIYPKPVLDLTKETADFILAKMNYKL"
BASE COUNT          831 a         1017 c          863 g          656 t
ORIGIN      
        1 tggtccccgc ggcacatttt tcctattcgt acctcagggc ctcgatcgga tttaactttc
       61 ccgccttgta agccggatac gcccccgccg ccagacctac aacggtgcac attgcaatac
      121 ccagcaacag ccagatccat ggaagcacga agccggtcga gagaacgatc gaaaacgtat
      181 ttcccacgac cacccccagc acgacgccga aggcggctcc gatgagactg atgatcacgg
      241 attcaaaaag gaattgttgc cgcacgctcc gttgccgccc gccgatcgcc ttaaccagcc
      301 cgatctcctt tgtccgttcg gttaccgcaa cgagcatgat attcatcagg cctaccgccg
      361 cgcctaccag ggtgatcacc ccgatgacaa ccgccgccca ggtgataaaa ctgaggttct
      421 taaggaggag gtttacaaag gtgtcgctct tgtcgatcag gaaattatcc gcttccgtca
      481 cgtccagccg ccgcaccggc cggaagatgc cctcagcctg gtcgatggcc ccgtcgatct
      541 gccgcacatc cgggaccttc acctggatat tgaaggatgt cgccgaaccg gtgctgaaca
      601 agcgccgcac gttattataa gacgtgagga tcatattgtc atagttcatt cccagggtac
      661 tgcctttgga agccagcacg ccgacgaccc ggaacggcag gttattgata tggatgattt
      721 tctcaacggg ccgctcggga ttttcgccaa agaacttcct ggcgatcgca cttccgatca
      781 cacagacatt cctgcccgat tggatgtcca gctggttgag gttccgcccc gctgccaggg
      841 taaacccgtt gaggtcgata tagttcgcgt ccccaccctg tacgcggatg ttcggattgg
      901 ttttctggtc accgacagag accaccgcat cgccgacgcc cgatatcccc aacgagaccg
      961 tcgcgggata attgtaccgc gcaaggaagg tctcggcctg cagcttcgtg atgggctcat
     1021 tggtatttga ccgcttttcc tttaaagagc cccgcttgga cgatttaacg ccgtcgccgt
     1081 gaaaattgaa ccagttcttg tagtggatgg taaacccgtt tgcgcccatc gccgagaagc
     1141 tttccgtgaa tttctgtttc atcgcttcga tggcggtatt gatgccgacc agcgccatga
     1201 tcccaaacgc aatgatggcc accgtaatac ccgtccgcag ccgattcccg cggatcgtcc
     1261 ggtaagccag tgataaagta tcgctaagtc gcatggtgag gaaagcaatt taaagaattt
     1321 tccgctaccc cggagcccgg ccggttacca agtataaatt agcctcgaaa gctgccccag
     1381 cagggactgg gggaagacgc ccaaaaccag tacaaggact gccagcacca ccagcattcc
     1441 tttataccct gcccccggcg ccgcctcggt cgtgccgtcc ccgtccttga agtacatggc
     1501 ctggatcacc cggaaatagt agtaggcgct caccgccgcg cagagtacgg caaataccac
     1561 cagccaaagg aacctgcccg tcgataccac cgacgccagc atatagtatt tggcaaagaa
     1621 accgccggtc agcggaatgc ccgccagcga caagagaaag atcgtacagg tggcggccag
     1681 cagcggctgc tggcgcgcaa ggccattaaa cccctcaaac gtgtagtcct tcatccggat
     1741 caacaccgcg aacacgccaa tcgtcgcgat actgtacgcc gccgcataga ggataatgcc
     1801 ctccttcgcc acccccgaga gggataacag ggccagcagc ataaaaccag cctgcgcaat
     1861 ggaggaatac gccagcatcc gcttcacgct ctgctgaaag accgccgtga tattgccgat
     1921 gaacaaggtc gcggccgcaa tgatcgccac aaagatttgc cattgtgccg ggtcggtgcc
     1981 gaacgcttcg tcgaagagcc ggacgaatcc cacaaaaatc gagaccttta cgatcgtcgc
     2041 catgaaagag gtaaagaccg tcggggctcc gtcatagacg tcgggcgtcc agaaatggaa
     2101 gggcgccgcc gaaaccttga aggcgatgga aacgagcagc aacaggagcc ccgataacaa
     2161 cagcggcgta accgccggtt tggcggtcca gatctcgtcg atattgaagg tgccggccgg
     2221 gctggcgcca tagaggaagg cgatgcccag cagcatcagg ccggtggaaa aggaccccag
     2281 caggaaatat ttcaacgcag cttcgttgct tttcaagttt cgcttgtcgc tccccgttag
     2341 gatataaagc ggtatcgaaa cgatctcgat tcccaggaaa agcatgagga gcgaattgaa
     2401 tgtggtcacc agggcgattc cagtaagggt aaaaaagacc agcgcataat actcggccag
     2461 gttgtgcccg attttttcca tatccttccc cgacaacagg accagcaaca aagtgcagcc
     2521 gaaagcaatg ctgttcccga gcagcccgaa gctgtcaaaa aagagcatct tgtgaacgtc
     2581 cataacgatc agatggtaac cctccaggtc ggcataattc ccgatcagca gcgccaccag
     2641 cccgatgatc gccaggttcc tcacggcggc tttccgcccc gtgaagatgc cgctgaacat
     2701 cataataatc ccccaaacag ccgaaatcaa tattgcgttc ataatttata attcatcttc
     2761 gccagaataa aatccgccgt ctccttcgtc agatccaaaa caggcttcgg atatatcccg
     2821 ataaaaacaa tcaggacgac gatcaccgcc aaagccaatt tctcatttag cctgatgtca
     2881 gtgacgcgtg aagtaagggc gttgacgtcg ccgaaaaaga cgtttttgat catattcagg
     2941 gtgtacaccg ctgccaggat caccgtcacc gcgccgacta ccgtgaagag cacattgtat
     3001 tgggtcgccg cagaggtgaa gacgcccgtg aacaacagga actcgccgat aaatccattc
     3061 gtcagcggca gtgcgatatt tgccagcgca acgacaacca gcaggatcga cagcccgggc
     3121 gccgttcgag ccagtccgcc cagctgggag atcttccggg ttccggtctt ccgttcgatc
     3181 agctcgacga ccacccacat cccgagtata ttgatgccgt ggttgaacat ttggatcatg
     3241 accccctgca tcccaatcga cgtcgtcgca aagatcgcga gacacatcag acccacgtgc
     3301 gcgatcgaag aataggcgac caaccgcttc aggtcgtcct gccggatcgc caccagcgac
     3361 gcataca
//
LOCUS       DATGKE010000110         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_388694, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000110 DATGKE010000000
VERSION     DATGKE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388694"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1237)
                     /locus_tag="VKU83_01955"
     CDS             complement(149..1237)
                     /locus_tag="VKU83_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HLI92338.1"
                     /translation="MRIGILGTRGIPNAYGGFEQFAQYLALGLHRRGHDLYVYNSSDH
                     PYREAEWNGVHIVHCRNWEHKIGTAGQFLYDLNCFRDARRRNFDILLQLGYTSSSVWH
                     RFWPGNAINVINMDGLEWKRSKYNRLTRKFLRRAEGLAVRHGDILVADSIGIKDYIQA
                     EYGRSSVYIPYGAEIPEKFDAEIPARYGLECGGYSLLMARMEPENNIEMIIRGWLASD
                     KTRPLVLVGNTGNSFGQYLVRTYQHKQLHFTGAIYEPEVIHALRHFSALYLHGHSVGG
                     TNPSLLEAMACGCVIAAHDNPFNRAILGDQAYYFSSAKQVCDALERNSAVEEKLAWQE
                     KNREKIKNIYNWDKIVGDYENVFRSQAR"
     gene            1597..2358
                     /locus_tag="VKU83_01960"
     CDS             1597..2358
                     /locus_tag="VKU83_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLI92339.1"
                     /translation="MFEARVREKKTTGEKDEIDVATISLRKYISNRSSYLLAKRVFDV
                     VLSLIVIIFVFSWLFPIIWVLIRMDSRGPVFFIQKRVGFLGKSFSCLKFRTMYVNEEA
                     NSRQATENDPRITRVGRFLRLSNLDELPQFINVIAGHMSIVGPRPHMHKDCNAFSRDV
                     NFYKFRNLMKPGITGLAQVKGYRGPAQNFEKIFRRYQWDAFYIRNAGFWLDLRIIHQT
                     GIETAAYLCNKAVSLGKLSKLARHSEWIKKEEVLN"
     gene            2588..>3096
                     /locus_tag="VKU83_01965"
     CDS             2588..>3096
                     /locus_tag="VKU83_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLI92340.1"
                     /translation="MQRTVVESAVCYPENWQKGTWKFIREYWSDRYNYLIYKRLMDIL
                     TSLAVIILVFPWLFPLIMLLIRLDSRGPVFFRQKRVGFLGRTFWCYKFRTMYVNADAD
                     TRRASSDDPRVTRIGWFLRNTCLDEIPQFINVLFGQMSIVGPRPHMLKDTLEFSNLVS
                     NYHFRNMARP"
BASE COUNT          737 a          805 c          726 g          828 t
ORIGIN      
        1 gtgctcacca ccgttacgct cactcccagg tcccgcaggc attgcgctgt ctgcatgatc
       61 tggatggtgt ctcccccttt gttggagaac agcgtggatc ttatgataaa caatactttc
      121 attgtcttgc aggccgcgcc cgcatccgtc atcttgcctg cgaccggaag acgttttcat
      181 agtcgccgac aattttatcc cagttatata tattcttgat cttttccctg tttttttcct
      241 gccacgccag cttttcttcc acggcgctat tccgttccag ggcgtcacaa acctgcttcg
      301 ccgatgaaaa ataataagcc tggtcgccca gtatcgcccg gttaaaggga ttgtcatgag
      361 ctgcaatgac gcatccgcac gccatggcct ccagcaatga gggattggtg cctcccaccg
      421 aatgcccatg aagataaaga gccgaaaaat gccgtagcgc atggatgact tccggttcat
      481 agattgcgcc ggtaaaatgc agctgtttgt gctggtaggt cctgacaaga tactgtccaa
      541 aggaatttcc ggtgttcccc accagtacca gcggtctggt cttgtcggag gccagccatc
      601 cgcggataat catctcgata ttgttctccg gctccatccg ggccatcaag agcgagtagc
      661 cgccgcactc aagcccgtac cgggcgggaa tctccgcgtc gaatttttcc gggatctcgg
      721 ctccgtaagg gatgtagacg gatgacctcc catattccgc ctggatataa tccttgatgc
      781 caatcgaatc ggctaccagg atatcgccgt gccttacagc caggccctca gcccttcgca
      841 ggaatttccg ggttagccgg ttatacttgc tccgtttcca ttccagccca tccatattga
      901 tgacgttgat ggcattaccg ggccagaacc tgtgccagac cgaactgctg gtatagccta
      961 gttgcagtag aatatcaaaa ttccgccgtc tcgcgtcccg gaaacaattc agatcataca
     1021 ggaattgccc ggcggtaccg atcttatgct cccaattccg gcaatgcaca atgtgcacgc
     1081 cattccattc agcctcccgg tatggatggt cgctggagtt ataaacatag agatcgtgtc
     1141 cgcgccggtg caggcccagg gccaggtatt gtgcgaactg ttcgaatccg ccataggcat
     1201 tcgggattcc ccgggttcct aagatgccga tcctcattcc gttaaaaatt ttgcaaatgt
     1261 aattgattcc tatccgactg tgcgctttga atcaattatt tcagcatctt tgcactcgag
     1321 ctggaattta tcctactgaa ggtcaattgt tttttgccct ttaccgcatt gcctacttcc
     1381 cgtcccctcc gcctttctgg ttcgccgtgc aataagttgg ataattatct gtttataagt
     1441 gtcttcgatg gaatggccct gcataagagg aaccatgtat tcgtcgcaaa taactataaa
     1501 ctgaaaccgc attaaaccat taactatcgt atcggcaaat gtcgcgcccg ccatctgcgg
     1561 cattgtctga tacctaaact aacaaaccaa agccttatgt tcgaagccag ggtaagggaa
     1621 aaaaagacta caggagaaaa agatgagatc gatgtcgcca ctatcagttt gagaaaatat
     1681 atcagtaacc gcagcagcta tctccttgcg aagcgggtgt tcgacgttgt cctttcgcta
     1741 attgttatca tcttcgtatt cagctggtta tttccaatca tctgggtgtt gatccgaatg
     1801 gattcacgcg gcccggtctt cttcatccaa aagcgtgtcg gctttctcgg caaatcattt
     1861 tcctgtctga agttcagaac gatgtatgtc aacgaagagg ccaacagcag gcaggcgaca
     1921 gaaaacgatc cccgcatcac ccgggtaggg cgtttcctgc ggctttcgaa cctggatgag
     1981 ctgccccagt tcatcaatgt gatcgcaggc catatgagca tcgttgggcc gaggccacac
     2041 atgcataagg attgtaatgc tttttcgcgg gatgttaatt tttacaaatt ccggaatctg
     2101 atgaagccgg ggataaccgg tctggcacag gttaaaggat accggggccc cgcgcagaac
     2161 ttcgagaaga tcttccgccg gtatcagtgg gacgctttct acatccggaa cgccggtttc
     2221 tggctcgacc ttcggatcat tcaccaaacc ggaatagaaa ccgcggctta tttgtgcaac
     2281 aaggcggtat ccctcggaaa actgtcgaaa ctcgcccggc acagcgaatg gatcaaaaag
     2341 gaagaagtgc ttaactaaaa attaacgcat gtcgttttcc aatattcgtt attaaggaac
     2401 ggacgaatgc aacgttattg taacagttct tgtcaacatt cggaacgtca accgcacctg
     2461 taatggttcg cagcttgccc ctgtttaagt cttaattcct gcctccaatg gcaccgttat
     2521 tgctaacacg acattgagat gatggggata ctaatcaaag ttgagtttaa aaccaatcct
     2581 tcgtaacatg cagcgtactg ttgttgaatc ggccgtctgt tatccggaaa actggcaaaa
     2641 aggaacctgg aaatttattc gcgagtattg gagcgatcga tacaattatt tgatatacaa
     2701 gagattaatg gatattttga cttctcttgc cgtcattatc ctggtttttc cctggttgtt
     2761 cccccttatc atgctgctga tcaggctgga ttcaagggga cccgtttttt tccggcagaa
     2821 aagggtcggc ttccttggga ggaccttctg gtgctataag ttcaggacga tgtatgtcaa
     2881 tgccgatgcc gatacccgcc gggcttcttc cgatgatccg cgcgttacgc ggatcggctg
     2941 gttcctgcgc aatacctgtc tggatgaaat cccgcaattc atcaatgtat tgttcggtca
     3001 gatgagcatc gtcggcccgc gtccccatat gttaaaagat accctcgaat tttccaacct
     3061 ggtttcaaat tatcattttc gcaatatggc gaggcc
//
LOCUS       DATGKE010000111         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_512986, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000111 DATGKE010000000
VERSION     DATGKE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512986"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VKU83_01970"
     CDS             complement(<1..517)
                     /locus_tag="VKU83_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI92341.1"
                     /translation="MKITVIDAGKRFNRDWIFRHLSYEFSTGHSYAITGPNGSGKSTL
                     LQSLAGAIGISEGAIHYADAGKTAEHRQTLTGPGKTLAALAQIPADQIFRHLSLAAPY
                     LQVIEEMTLTEFLHFHDAFKPLGCTVKEAIARIGLEASAHKQIRFYSSGMKQRVRLAQ
                     AFFSDTPIVLLD"
     gene            complement(498..1304)
                     /gene="lpxA"
                     /locus_tag="VKU83_01975"
     CDS             complement(498..1304)
                     /gene="lpxA"
                     /locus_tag="VKU83_01975"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLI92342.1"
                     /translation="MIHPLASVHPEARLANDVHVDPFTMIYKNVEIGSGTWIGSNVTI
                     MEGARIGKNCRIFPGAVVSGDPQDLKFDGEETTVEIGDNTTIREFVTIHRGTRDRWTT
                     KVGRNCMILAYSHIAHDCIIGDNCVLSNNSQVAGHVVMGDWVILGGMCAIHQFVRVGS
                     HVFLGGGSLVGKDIPPYIKAARMPLSYSGVNSVGLKRRGFTIDKINHILDIYRVIYNK
                     GMNTSQALEYLEEEFVASDERDEIVGFIRESGRGIIKRYTKTSFDEDYGD"
     gene            complement(1501..>2109)
                     /gene="fabZ"
                     /locus_tag="VKU83_01980"
     CDS             complement(1501..>2109)
                     /gene="fabZ"
                     /locus_tag="VKU83_01980"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HLI92343.1"
                     /translation="KLLDVIGDLALIGYPIKGHIMANRPGHATNVEFAKKIKQYIKKN
                     KHVKNTPVYDPNQPPVFNTPQIEKTLPHRFPFLLVDKIIELTDRTIVGIKNVTFNEPF
                     FVGHFPGNPVMPGVLQIEALAQTGGILCINAMPQGEYDTYFIKIDNCKFKHKVVPGDT
                     MILKMELLNPIRRGICEMRGTVFVGNKVATEADLMAQLVKRS"
BASE COUNT          431 a          521 c          624 g          533 t
ORIGIN      
        1 cgtcgaggag gacgatgggg gtatcggaga agaaggcctg ggcgagccgg acacgctgct
       61 tcatgccgga ggaataaaag cggatctgtt tgtgggcgga tgcttcgagg cctatccggg
      121 cgatggcttc cttcaccgta cagcccaggg gtttgaaagc gtcgtgaaaa tgcaggaatt
      181 cggtgagcgt catttcttcg atgacctgaa ggtaaggagc ggccagcgag aggtggcgga
      241 agatctggtc tgccggaatc tgcgcgaggg ccgccagggt cttcccgggg cctgtgaggg
      301 tctgccggtg ttctgccgtt ttgccggcgt cggcgtagtg aatggctccc tcgctgatgc
      361 cgatggctcc cgcgagggat tgcaacaggg tcgatttgcc cgatccgttg ggaccggtga
      421 tcgcgtagct atggcctgtg ctgaattcgt aactgagatg gcggaagatc cagtcgcggt
      481 tgaaccgttt gccggcgtca atcaccgtaa tcttcatcaa agctcgtttt ggtgtagcgt
      541 ttgataattc cgcgaccgct ttcccggatg aaaccgacga tctcgtcgcg ttcatcgctg
      601 gcgacaaact cttcttccag gtattcgagc gcctggctgg tattcatccc tttattgtag
      661 ataacccggt agatatcgag gatgtgattg atcttatcga tcgtgaatcc gcggcgtttg
      721 aggccgacgg aattgacgcc ggaatagctg aggggcatac gggcggcctt gatgtaaggg
      781 gggatatcct ttcccaccaa agagccgccg cccaggaaga catgggagcc cacccgaacg
      841 aactgatgga tggcgcacat gccgccgagg atcacccagt cgcccattac gacatggccg
      901 gccacctggg agttattgct caggacacag ttgtcgccta tgatacaatc gtgtgcgata
      961 tggctgtaag ccaggatcat gcaattgcgt ccgacctttg tcgtccatct gtcacgggta
     1021 ccgcgatgga tggtgacgaa ttcgcggatg gtcgtattgt cgccgatctc tacggtagtc
     1081 tcttcgccgt caaacttaag gtcttgagga tcaccggata caacagctcc cgggaatatt
     1141 ctgcaattct ttccgatccg tgccccctcc atgatcgtga cattgctgcc tatccaggtt
     1201 cccgatccaa tttcaacgtt cttatagatc atcgtgaacg ggtccacgtg tacatcgttc
     1261 gccagacgcg cttcggggtg aacagaggcg agagggtgga tcattctacg agcaattgat
     1321 tgattttcag tttcttggat cattatttga ggcttccttg cggtttgctg ttttcttcaa
     1381 aaaaagtgcc gaaattctcg tttctgcgac tggcacagac ttgccctcag gcttacgcct
     1441 gtcacggtca ttttacaaaa ataaacttat ttgccacaat taccggaaat atcctcgtgt
     1501 ttacgaccgc ttaaccagtt gggccatgag gtccgcctcg gtggcgactt tattgccgac
     1561 aaagacggtc ccgcgcattt cacagatgcc gcggcggatg gggttgagga gctccatttt
     1621 caggatcatc gtgtcgccgg gaacgacctt gtgcttgaac ttacagttgt cgatcttgat
     1681 aaaataggta tcgtattcgc cctgcggcat ggcattgatg caaaggatac caccggtctg
     1741 ggcgagcgct tctatctgca gaaccccggg catgacgggg ttaccgggaa aatggcccac
     1801 aaaaaagggt tcgttgaagg tgacgttctt gatgcctacg atagtccggt cggtcagctc
     1861 tatgatcttg tccacgagca ggaaggggaa gcggtggggc agggtctttt cgatctgggg
     1921 cgtattaaaa acgggcggct ggttaggatc gtagacgggt gtattcttaa cgtgtttatt
     1981 cttcttgatg tattgcttga tctttttggc gaattcgaca ttggtggcgt gtcccggccg
     2041 gttggccata atgtgtccct tgatcggata gccgatcagc gccaggtcgc cgatgacgtc
     2101 gaggagctt
//
LOCUS       DATGKE010000112         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_696252, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000112 DATGKE010000000
VERSION     DATGKE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696252"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="VKU83_01985"
     CDS             <1..1738
                     /locus_tag="VKU83_01985"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HLI92344.1"
                     /translation="SELSVCGYPPRDFLEFEDFISKCYAMVDRIREETAQIGVIVGSP
                     ARNPTGKGKELFNAAWLLYEKEVKGVAHKTCLPNYDVFDEYRYFEPGFDWKVIPFKGK
                     RIALTICEDIWNMGDHPLYRVCPMDELIGQDPDLMINISASPFDYDHDEDRKEVVRQN
                     VLKYGLPIYYCNAVGSQTEIVFDGGSVIYDAKGKLVKELKYFEEDFAVIPLEESPQEN
                     GGASGKKPIPPLYEELLPGLSVAVTDEPEPGHRQHRAGHGVVPGGIVTRIFDKDMRVS
                     KLNDPDRILQYLTNDENISQIRQALILGIRDYFFKMGFRKAILGSSGGIDSAVTLTLA
                     CEALGRENVRAILLPSPYSSAHSVSDAEQLSRNLGNPYDIIPVREVYEALLHTLEPVF
                     RDASKGSQSPDIGLAEENLQSRTRGNILMGLANKFGYILLNTSNKSELATGYGTLYGD
                     MAGGLSVLGDVYKGQVYALARCLNRNGGMIPPNILEKAPSAELRPGQKDTDSLPEYDV
                     LDKILYQYIERRQGPKEIIAMGIDEALVARVLRLVNTNEYKRNQFCPIIRVSCKAFGV
                     GRRVPIVGKYLS"
     gene            complement(1775..2230)
                     /locus_tag="VKU83_01990"
     CDS             complement(1775..2230)
                     /locus_tag="VKU83_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92345.1"
                     /translation="MHANWTIFTLMLTAGLSLMACSARNGSRKDLELLTNSPWKYEKA
                     GFDSNDDGIFDALNPAIAGSEKDNMIIFCQDGTGYSALGPSSRFGSRSVRGALDSLPF
                     LWQFQNNDSTIYFQDRYYKVRKLDRQHLEMYADEKFGGVSTRYVIVLRH"
     gene            2380..3381
                     /locus_tag="VKU83_01995"
     CDS             2380..3381
                     /locus_tag="VKU83_01995"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI92346.1"
                     /translation="MLYTAEDIRQLNNKIQYQASFIDRLRDEVGRVIVGQHQMLDRLL
                     IGLLSHGHILLEGVPGLAKTLTIKSLAQAVHARFSRIQFTPDLLPADVIGTMIFNQQK
                     HEFVVRKGPIFANFVLADEINRAPAKVQSALLEAMQERQVTLGDSSHPLEEPFLVLAT
                     QNPLEQEGTYPLPEAQQDRFMMKVIVDYPTKQEEQLIIRQNVQGLQSPGISQVVSTQE
                     IIQAKELTRQIYMDEKVEHYILDIVFATRTPDKYKLEKLKPLIAYGGSPRASINLALA
                     AKANAFLNKRGYVIPEDVRSISKDVLRHRIGLTYEAEAENVNVEQVVDDVLKAIPIP"
BASE COUNT          848 a          950 c          951 g          700 t
ORIGIN      
        1 ctcggagctg tcggtctgcg gctacccgcc gcgggatttc ctggaatttg aagacttcat
       61 ctccaaatgc tatgcgatgg tcgaccggat cagggaggag acggcgcaga tcggcgtcat
      121 cgtcggcagc cccgcgcgca atcccaccgg aaagggaaag gaactcttca atgctgcctg
      181 gcttctatac gaaaaagagg tcaaaggggt ggctcataaa acatgccttc cgaactacga
      241 tgtcttcgac gaataccgct atttcgaacc cggcttcgac tggaaggtca ttcctttcaa
      301 agggaaacgc atcgcgctta cgatctgcga ggacatctgg aatatggggg accatcccct
      361 gtaccgggtt tgccccatgg acgagctgat cggccaggat ccggacctca tgatcaatat
      421 ctccgccagc cccttcgatt acgatcatga tgaggaccgc aaggaagtgg ttcgccaaaa
      481 cgtgctgaaa tatggccttc ccatatacta ctgcaacgca gtggggtcgc agaccgagat
      541 cgtcttcgac ggaggcagcg tcatctacga cgccaagggc aagctcgtga aggaactcaa
      601 gtattttgag gaggatttcg cggtgatccc tctcgaggaa agcccgcagg aaaacggcgg
      661 ggccagcggg aagaaaccca ttccgccctt atacgaggag cttcttcccg gcctctcggt
      721 ggccgtcacg gacgagccgg aacccggaca ccgtcaacat cgggcaggtc acggcgtcgt
      781 tccaggaggc atcgtaacgc ggatcttcga taaggacatg cgggtctcca aactgaatga
      841 cccggaccgc atcctccaat acctcacgaa cgacgaaaat atttcccaga tccgccaggc
      901 attgatactc gggatccggg attatttttt taagatggga ttcaggaaag ccatccttgg
      961 aagcagcggg ggcatcgaca gcgccgtcac gctgacgctg gcctgcgaag ccctgggcag
     1021 ggagaatgta cgcgccatcc ttctgccgtc accctactcg agcgcccatt cggtaagcga
     1081 cgccgaacag ctttcgcgca acctcgggaa cccgtatgat atcatcccgg tccgcgaagt
     1141 gtatgaggcc ctgctccaca ccctcgagcc ggttttcagg gatgcctcga agggcagcca
     1201 gtcaccggat atcggacttg ccgaagagaa tctccaaagc cgtacccggg gaaatatcct
     1261 gatggggctt gccaacaaat tcggctatat cctcctgaac acgagcaaca aaagtgagct
     1321 ggcgacgggc tacggcacgc tgtatgggga catggcgggc ggccttagcg ttctggggga
     1381 cgtttacaag ggacaggtgt atgcccttgc ccgctgcctc aaccggaacg gcgggatgat
     1441 cccgccgaat atcctggaga aggccccctc cgccgagctc cggccggggc aaaaggatac
     1501 cgacagcctg ccggaatacg acgtcctgga taagatcctt taccaatata tcgagcgaag
     1561 gcagggtccc aaagagatca ttgcgatggg catcgatgaa gcgctggtag cgcgggtgtt
     1621 gcggctcgtg aataccaacg aatacaagcg gaatcagttc tgcccgatca tccgggtgag
     1681 ctgcaaggca ttcggcgtag ggcggcgggt acctattgtc ggaaaatacc tgtcctgatc
     1741 cgcggactgc ccggagaacg gccggcagcc gccgtcaatg tcttaagacg atgacatacc
     1801 gggtgcttac gccgccgaat ttctcatctg catacatttc gagatgctgc cggtcgagct
     1861 tcctgacctt gtagtaccgg tcctggaagt aaatcgtact atcgttgttc tggaattgcc
     1921 acaggaaagg caggctgtca agagcgcccc tgacggaccg gctgccgaag cgcgaagaag
     1981 gtcccaatgc cgaataacct gttccatcct gacaaaatat gatcatattg tctttctcgc
     2041 tgcccgctat cgccgggttg agagcatcga agatgccgtc gtcgtttgaa tcgaagccgg
     2101 ccttctcata tttccacggt gaattcgtga gaagctcgag atccttcctt gaaccgttcc
     2161 gggccgaaca agccatcagg gaaagcccgg cggtcaacat cagggtgaat atggtccaat
     2221 tcgcatgcat cttcacaggt ttccattaga agggtggcga tcatcaaagt ttaaaactcg
     2281 gggatcaaat aattatttca tgtaaatccg ataaaaattt ttaggggatc tttgccaaat
     2341 cgaattcgta ctttcggcct ttatataatc ttcagcgata tgctttatac agcagaggat
     2401 atcagacagt tgaacaacaa aatccagtac caggcgtcat ttatcgaccg gctgcgggat
     2461 gaggtgggaa gagtgatcgt aggtcagcat caaatgctcg accggctgct gatcgggctg
     2521 ctcagccacg gccacattct cctggaaggg gtgccgggat tggctaaaac attaaccatc
     2581 aagtcattag ctcaagcggt ccatgcccgg ttcagtcgca ttcaatttac tcctgatctc
     2641 ttgcccgccg acgttatcgg gaccatgatc tttaaccagc agaaacacga gtttgtggtc
     2701 cgaaagggac ccatttttgc aaatttcgtt cttgcggacg agatcaaccg cgctcccgca
     2761 aaggtccaaa gcgcgctgct ggaggcgatg caggagcgcc aggtgacgct gggcgacagc
     2821 agccacccgc tggaagagcc tttccttgta ctggcgacgc aaaaccccct ggagcaggaa
     2881 ggaacctatc ctctgccgga agcgcagcag gaccggttca tgatgaaggt gatcgtagat
     2941 taccccacca agcaggaaga acagctcatt atccggcaga acgttcaggg gcttcaatcc
     3001 cccggcatca gccaggtggt gtcgacacag gagatcatcc aggcaaagga gcttacccgg
     3061 cagatctata tggacgagaa agtcgaacat tatatcctgg acatcgtttt cgccacgcgg
     3121 acgccggaca aatataagct cgagaaactg aaacccctca tcgcatatgg cggcagcccc
     3181 cgggcaagca tcaaccttgc actggcggca aaggccaacg cttttctcaa caagcgcggt
     3241 tatgtcatcc cggaggacgt ccgctctatc tcaaaggacg tcctcagaca ccggattggg
     3301 cttacctacg aggcggaggc ggagaatgtc aatgtagaac aggtggtcga cgatgtcctc
     3361 aaagccattc cgatcccctg ataattcaga ccatgctgac gacagccgaa atattgaaga
     3421 aggtaaggga gctggacatc aaaagcaag
//
LOCUS       DATGKE010000113         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1424095, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000113 DATGKE010000000
VERSION     DATGKE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424095"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VKU83_02000"
     CDS             <1..433
                     /locus_tag="VKU83_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92347.1"
                     /translation="ITSMRRQHYATMKDISWANYYMGLNLPNTVGLMRKTPFPATIPV
                     IDLVSEINFPTPAWSGRWADCHRQLVNAQPGYREGITAHGCGHFIFRDNPPLVIGAIV
                     KAYTGVLTKEQRDEIMNRYLSYSLENANEEKRREVHQAKKN"
     gene            467..2659
                     /locus_tag="VKU83_02005"
     CDS             467..2659
                     /locus_tag="VKU83_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLI92348.1"
                     /translation="MKNAVFVLLLAVQIGAQAQTSAQVQPPVQAGARTQAQPSYPFQN
                     RDLPVEKRIDNLLSLLTMDEKVTCLSTNPTIPRLGLKGTGHSEGLHGLALGVPGGWGR
                     KTPVATTTFPQSIGMAETWDTALIHEAAAIEGYEARYTNQTLAKGGLVIRAPNADLGR
                     DPRWGRTEECYGEDAFFNGTLVQAYVHGLQGDDPRYWQAASLMKHFLANSNENSRDSS
                     SSDFDERLWREYYSVPFRMGVEAGSRAYMASYNKVNGIPQAVNPSLRKITVDEWGQNG
                     IICTDGGAYRLLLTAHHYFKTPEQAAAACVKAGINQFLDNYRAGVKGALDEQLLTEKD
                     LDTVLRGVFRVMIKLGLLDDSTLVPYASIRGGSPPFMDPQHKQFVRLLTQKSIVLLKN
                     TDHFLPLDRQKLHSIAVLGSRSDQVYGDWYSGSPGYAVSPLQGILNKLGPDLSVNHTT
                     NNDLAVSLARSSDVAIVCVGNHPTGGENKGWKQVSVPSEGREAVDREQIDLDSAQESL
                     VERVMAVNPRTIVVLISSFPYSINWINEHVPAIVHLAHNSQELGNALADVLFGDVDPG
                     GRLVQTWPTSLDQLPPMMDYDIRHGRTYMYFKGKPLYPFGYGLSYTTFRYDNLRLSSP
                     VLKDALSISVDITNTGDRIGDEVVQLYVSHENSKVIRPIKELKGFSRITLRPNETKTV
                     TFPIAPRDLTYWNAAHHRFETEKDTIKVMVGGSSAPADQKATASFKVL"
BASE COUNT          647 a          812 c          703 g          551 t
ORIGIN      
        1 tatcacgagc atgagaagac agcattatgc aaccatgaaa gatatcagct gggcaaatta
       61 ctacatgggc ctaaatcttc ccaataccgt ggggctgatg cggaaaacgc ctttcccggc
      121 gaccatcccg gtaatcgatc tggtttcgga gatcaacttc cctactcccg catggtccgg
      181 cagatgggcg gactgtcacc ggcaattggt aaacgcgcag cctggctacc gcgagggtat
      241 aaccgctcat ggatgcggac attttatttt cagggataac ccgccccttg tcataggcgc
      301 catcgtaaaa gcgtataccg gtgtgctgac caaagaacaa cgcgatgaaa taatgaaccg
      361 gtatctttcc tatagcctgg agaatgccaa tgaggagaag aggagagagg ttcatcaggc
      421 gaaaaaaaac taaatatcct ctatctttag tggacaatgc cttgccatga aaaatgctgt
      481 ctttgtcctt ctcctcgccg ttcaaatcgg ggcccaagcc caaacatcgg cccaggtcca
      541 accgccggtc caagccggag cccggacgca ggcccaaccc tcctacccct tccaaaaccg
      601 ggaccttcct gtcgaaaaac gcatcgacaa cctgctttcc ctgcttacca tggacgaaaa
      661 agtcacttgt ctgagcacca acccgacgat cccccgtttg ggcttaaaag gaaccggtca
      721 cagcgaaggg ctgcatggcc tcgcgctcgg agtccctggc ggctggggaa gaaaaacacc
      781 tgttgccacc actaccttcc cgcagtcgat cggtatggcg gagacctggg acacagcgct
      841 gatacatgag gcggcagcca tcgaaggata cgaagcccgc tataccaatc agacgcttgc
      901 caaaggcggc ctcgtcatcc gggccccgaa cgccgacctc ggccgcgacc cccggtgggg
      961 aaggaccgaa gaatgttacg gtgaagacgc cttcttcaat ggcaccctgg tacaggccta
     1021 tgtacacggc ctccagggtg acgatccccg ttattggcag gctgcttcgc ttatgaaaca
     1081 tttcctggcc aacagcaatg aaaacagccg ggacagcagc tcttccgact tcgacgaacg
     1141 gctctggcgc gaatactact cggtgccctt ccgcatgggt gtcgaggccg gctcgcgcgc
     1201 ctatatggcc tcgtataaca aagtgaatgg catcccccag gccgtaaacc cgtcgttgag
     1261 aaagatcacc gtcgatgaat ggggacagaa tgggatcatc tgcaccgacg gcggcgccta
     1321 tagactgctc ctcaccgccc accactattt caagacgccg gagcaagccg ccgccgcttg
     1381 cgtcaaggcc ggcatcaacc agttcctcga caactaccgc gccggggtca aaggcgcatt
     1441 ggatgagcag ctgctcacgg aaaaagacct cgacacggtc cttcgcggcg tcttccgggt
     1501 aatgatcaag ttagggctat tggacgattc caccctcgtg ccctatgctt cgatccgtgg
     1561 cggctcgccg cccttcatgg acccgcagca taagcaattc gttcgcctgc taacgcagaa
     1621 atcgatcgtg ctgctaaaga ataccgatca ttttctgccg ctggacaggc agaagctgca
     1681 tagcatcgcg gtcctcggct cccgaagcga ccaggtctat ggcgactggt atagcggctc
     1741 cccgggctat gcggtgagcc cccttcaggg aatactaaac aaactggggc cggatctttc
     1801 cgtgaatcat actaccaata acgacctcgc cgtcagcctg gcaagatcat ccgatgtagc
     1861 catcgtgtgt gtcggcaacc atcccaccgg tggagagaac aaaggctgga aacaggtctc
     1921 cgtccccagc gaaggccgcg aagcggtaga ccgcgaacag atcgatctcg actccgcgca
     1981 agagagcctt gtggaacggg tcatggccgt caacccccgt accatagttg tgctcatcag
     2041 cagctttccc tattcgatca attggatcaa cgagcatgtg ccggcaatcg ttcatctcgc
     2101 acacaacagc caggaactgg gcaacgccct tgccgatgtc ctcttcggtg acgtggatcc
     2161 cggcggccgg cttgttcaga catggccaac ctcgctcgac cagctgcccc cgatgatgga
     2221 ctacgatatt cgtcatggaa ggacctatat gtatttcaaa ggcaaacccc tctatccctt
     2281 tggttatggg cttagctata ctaccttccg ctacgataat ctccgcctca gctcgcccgt
     2341 tttaaaagat gccctgagta tttcggtgga catcaccaat accggcgaca ggataggtga
     2401 cgaggtggtt cagctttatg tgagccatga aaattcaaag gtcatccggc ccatcaaaga
     2461 gctcaaagga ttttcccgta tcaccctccg ccccaacgaa acaaagaccg tcacttttcc
     2521 tatcgcgcca cgggatctga cctattggaa tgccgcgcac catcggttcg aaacggagaa
     2581 ggatacgatc aaggtcatgg tgggcgggtc ttcggccccg gcggatcaaa aggcgacggc
     2641 cagttttaaa gtcttatgag gggttgcggg tcaacgtggt gatcgcgttt tcattcactc
     2701 cttaagccct tta
//
LOCUS       DATGKE010000114         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1319116, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000114 DATGKE010000000
VERSION     DATGKE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319116"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..409
                     /locus_tag="VKU83_02010"
     CDS             224..409
                     /locus_tag="VKU83_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92349.1"
                     /translation="MKTLISLLTLVAIVFAYFLSATLYAQGELVSAYVLVMSSIAAIA
                     FWINSHDILRVQPVKKA"
     gene            464..1747
                     /locus_tag="VKU83_02015"
     CDS             464..1747
                     /locus_tag="VKU83_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HLI92350.1"
                     /translation="MSLIRALDRQRTIFLQGISGRRPLIPFQAAALRALARKKMSPRA
                     WAYIDGGAGNEESIQSNTTAFTAVKIRPRMMHPNDTADLSSSLFGMTLPFPLLLAPIG
                     VLDLICRSADRLVARQCRETNIPFIFSNQAGTPMEVCAGDMGETPRWFQLYWSRSDAV
                     VLSFVRRAEACGCQAIVLTVDTTLLGWRSRDLALGYLPFLSGMGIAQYTSDPVFLELL
                     RKGGGRDGIAGPRPAITPSTIATMLRLKQNYPGGLWKNIRSKEPLEAVRFFINTYSNP
                     ALSWDKLEWLREQTRLPILIKGILRADDAIRARASGADGIIVSNHGGRQVDGAISTLQ
                     ALVQIRRSLPRPFPVLLDSGIRSGSDMFKALALGADAVLVGRPYAYGLAIDRNNGVKE
                     VVRNLLNEFELTARLAGCRTLAEIGPDMVECGPSV"
     gene            1814..>2648
                     /locus_tag="VKU83_02020"
     CDS             1814..>2648
                     /locus_tag="VKU83_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92351.1"
                     /translation="MEKIPLWLAAIICIQSCSHSSLHLQTRDIWLCPQASVPPSPLNR
                     AVDLLQLFSPGAPWEKAASHTKVFKLYGSFVGPASQGQVDTIVADLNRRGIAIALEAG
                     VIDVDSRDPVPPCGGLGLVEGYGTPAQAMNICGKIKKAGGVISFLCMDEPLFYGHYFT
                     GRPGGGQPGCHSPVGDIVSLSVPTLNTYLQEFSDIVIGDIEPTEIAAFSDWQNDLAGW
                     ATDFRTAMGRPLAFIHLDIPWANGAPSKEPDDALAFYQYAGTLKTEHLLGKIGIIMNG
                     TP"
BASE COUNT          544 a          760 c          701 g          643 t
ORIGIN      
        1 gcactatgtt tgtatcactc ctcagagatt caatctccac tcggaaaacc tgcaatagac
       61 gtacccaatt ccctcgaaac gccttctcat tattctacca tttcgctgat ccttttacgc
      121 atttcctggt atcctttgta atgcctcgtt tccaatgact atgagccgtt catggcttat
      181 tctttttcta tcatagtctt taattaccta aatgtaccac aaaatgaaaa ctttgatcag
      241 cctcctcacc ctcgttgcaa tcgtttttgc ctatttcctg agcgctactc tttatgcaca
      301 aggcgaactg gtttctgcct atgtactggt gatgtcttct atcgccgcca ttgctttctg
      361 gatcaattcg cacgacatcc tgcgcgtgca gcccgttaaa aaggcgtaac ccacagcgac
      421 ccgccggaag ttgcgtatct tgtagccgat caacacgcta cttatgtccc tcatacgggc
      481 actcgatcgg caacgcacca tcttcctgca ggggatctcc ggaagaagac ctttgatacc
      541 ttttcaggcc gcggcgcttc gcgcgcttgc aaggaagaag atgtctcccc gcgcatgggc
      601 ttatatcgat ggcggcgctg gaaatgaaga aagcatccag tccaatacta ccgcgtttac
      661 cgcggtaaag atcaggccgc ggatgatgca tcccaatgac acggccgatc tttcctcctc
      721 tttgtttggc atgacactgc cgttcccgtt gctactggct ccgataggcg tcctggacct
      781 gatctgtcgc agcgcggaca gacttgtcgc caggcaatgc cgggagacca atatcccatt
      841 cattttttcc aatcaggcag gtactcccat ggaggtctgc gccggcgata tgggagagac
      901 tccgcgttgg ttccagctct attggagcag atccgatgcc gtagtccttt cctttgtgcg
      961 gcgcgcggaa gcgtgtggct gtcaggccat cgttcttacg gtagatacca ccttgctggg
     1021 atggcgaagc cgcgatctgg cgctgggcta tctgccattt ctgagcggta tgggcatcgc
     1081 ccaatatact tcggaccccg tattcctgga gctgctccgg aagggcgggg gcagggacgg
     1141 aatcgcgggt ccccgaccgg ccattacacc atcgaccatt gccaccatgc tccggctcaa
     1201 gcagaattac cccggcggtc tttggaaaaa tatccggagc aaagaaccat tggaagccgt
     1261 ccgttttttt atcaatacct actcgaatcc tgccctcagc tgggacaagc tggagtggct
     1321 gcgggaacaa accaggctcc ccatcctgat caaaggcatt ctgcgtgccg acgacgctat
     1381 ccgggcccgg gcttcgggcg ccgatggcat cattgtttcc aatcacggcg gccgccaggt
     1441 agacggcgcc atttcgacat tgcaggccct cgtacagatc cgccggtcgc ttccccgtcc
     1501 attccccgtt ttactcgaca gcggtatccg cagcggcagc gatatgttca aagcattggc
     1561 gctgggcgca gacgccgtcc tcgtcggccg gccctacgct tatggcctgg cgatagaccg
     1621 gaacaatggg gtaaaagaag tggtccgcaa cctgctcaac gaattcgagc tcacagcaag
     1681 gctcgcgggt tgcagaacgc tggccgagat cggtccggac atggtcgaat gcggcccctc
     1741 cgtttaacat cttattaagc ggatctattg accgggcaca ccggcaatgt tctattttcg
     1801 tgaaaacctt tctatggaaa aaattccctt gtggctcgcg gccattatct gtatccaatc
     1861 ctgcagccat agtagtttgc acctgcagac ccgggatatc tggttgtgcc cgcaggcttc
     1921 tgttcctcct tcgcccttga atcgtgcggt cgatctcctg cagctattct cgccaggcgc
     1981 accctgggag aaagcggctt ctcataccaa ggtcttcaag ctctatggca gcttcgtcgg
     2041 ccccgcttcc caggggcagg tcgatacgat cgtagccgat ctcaacagac ggggtattgc
     2101 gatcgcgctg gaagcagggg tgatcgatgt cgactccagg gatcctgttc ctccctgcgg
     2161 cggcctgggg ctggtcgagg gctatggcac tccggcccag gcgatgaata tctgtggaaa
     2221 aattaaaaag gccggcggcg tgatcagctt tctgtgcatg gacgagccct tgttctatgg
     2281 acactatttt acggggagac cagggggtgg acaacctggc tgtcacagcc ccgtcggcga
     2341 tatcgttagt ctgagtgtgc cgacattgaa tacgtacctg caggaattca gcgatatcgt
     2401 gatcggtgac atcgaaccta ccgagatagc tgcgttttcg gactggcaga acgatttggc
     2461 tggttgggct acggattttc gtacggcgat ggggcggcct ttggccttca ttcacctcga
     2521 tatcccatgg gccaacggcg ctccgagcaa agagccggat gacgcgctgg cgttctatca
     2581 atacgccggt acgttgaaga cggagcatct attgggaaag atagggatca ttatgaacgg
     2641 tacccctt
//
LOCUS       DATGKE010000115         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_801710, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000115 DATGKE010000000
VERSION     DATGKE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801710"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VKU83_02025"
     CDS             <1..826
                     /locus_tag="VKU83_02025"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HLI92352.1"
                     /translation="PVFNESIRGDMVFLIQSTCAPAENLLELLLMVDAARRASAYKVV
                     AVIPYYGYARQDRKDKPRVAIGSKLIANMLVSAGADRIITMDLHAAQIQGFFDIPVDH
                     LDSSAIFIPYIEQLKMENLTFAAPDVGGANRVREIASYFNAEMVICDKHRKRANEIAS
                     MVVIGDVTGKDIVLIDDLVDTGGTLAKSAGLLKEKGARSVRALCTHPVLSGNAYQNIE
                     NSVLDELVVCDTIPLKQAGKKIKVLSVAELFAVAIRNAYENRSITGLFIHSQLRGK"
     gene            848..1540
                     /locus_tag="VKU83_02030"
     CDS             848..1540
                     /locus_tag="VKU83_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLI92353.1"
                     /translation="MKTITIEGQLRTEIGKQATRQLRSEEKVPGVIYGGAKEVNFSAP
                     ATAFKTLVYTPDFQLAEIKVDGSAYRCILKDLQFDKVTDDLIHADFLELVEDKKVIAT
                     IPIKFTGAAKGVKDGGKLITKMKALKVKTYPKFLKENIEVDLTELELNGNVRVEDVKA
                     DNYEILNSPRIPIASVVLTRQLKQEEATATTPAAGAAPAAAPGAAGAKPAAGAPAAAP
                     AAAAAKPAGAKK"
     gene            1601..2182
                     /gene="pth"
                     /locus_tag="VKU83_02035"
     CDS             1601..2182
                     /gene="pth"
                     /locus_tag="VKU83_02035"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLI92354.1"
                     /translation="MPTKYLIAGLGNIGAEYEGTRHNIGFDILDAMAAKHQQFFTVDR
                     LAERVEIRWKGNLIVGIKPTTYMNLSGRAVKYWMGKDNITLDHILVVMDEIALPLNRF
                     RLRPGGSDAGHNGLTSIQEALGTQEYARLRFGIGSNFPRGRQIEYVLGKWTEHELPLV
                     RKKIEKSVELIESFASIGIERTMSLYNNLEFEL"
     gene            2347..2958
                     /locus_tag="VKU83_02040"
     CDS             2347..2958
                     /locus_tag="VKU83_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLI92355.1"
                     /translation="MKIFCVGRNYVAHAKELNNEVPEEPIIFMKPKSALLQAHTPFYY
                     PEFTNELHYECELVLRISKNGKYIQDRFASKYYDAVTVGVDFTARDIQTELKEKGLPW
                     EKAKAWDNSAAIGKWIPLQNIKNKKDINFCLYKNKELVQQGNSGLMINSFDKIVAYIS
                     NYFSVNIGDLVFTGTPAGVGECVVGDELEGFIEDDSLFTIEIK"
     gene            2979..>4080
                     /locus_tag="VKU83_02045"
     CDS             2979..>4080
                     /locus_tag="VKU83_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HLI92356.1"
                     /translation="MITDEILLKAYRLMRAAVEMAATYEKNRAVCRYVHSTSRGHEAV
                     QLATAFQLLPCDWISPYYRDESILLGLGFEPYDLMLQLLAKDGDIFTGGREYYSHPNY
                     RGSNHPRIIHQSSATGMQAIPATGLAQGLAFLERIGSPLLMRGPGGVLPIVVCSLGDA
                     SVTEGEVSEAFQFAVLKKLPIIFLVQDNNWGISVRADESRAMDSYEYAAGFKGMGRLR
                     IDGSDFQGSYETMGAVIREVRGGGGPFLVQAYVPLLGHHTSGVRREFYRDAADLAEHA
                     ARDPVPRLKAQLLFRGVAEEQLATIEDEVAQSIAAQFSRAVAAPEPVAATVGEDVFAA
                     TPVLEERGERTPAGREKVPMVDAALFAIRELME"
BASE COUNT         1013 a          974 c         1189 g          904 t
ORIGIN      
        1 accggtattt aacgaaagca tccggggaga catggttttc ctcattcaga gcacctgcgc
       61 accggcggaa aacctcctgg aattgctgtt aatggtagat gccgcacggc gcgcctccgc
      121 ctacaaggtg gttgccgtaa tcccctatta cggatatgcg cggcaggacc gaaaggacaa
      181 gcctcgcgtt gccattggta gcaagctgat agccaatatg ttagtttctg ccggggcgga
      241 ccgcatcatt acgatggacc tgcacgccgc gcaaatccag ggcttcttcg atatcccggt
      301 ggatcacctt gacagttcgg caatttttat cccttacatt gaacaactta agatggaaaa
      361 tcttaccttt gcggcccctg acgtgggcgg tgccaaccgg gtcagggaga tcgcaagcta
      421 tttcaatgcc gagatggtga tctgcgacaa acaccggaaa cgggcaaacg agatcgcaag
      481 catggtggtc atcggggatg tgactggaaa ggatattgtg ttgatcgatg acctggtgga
      541 taccggcggc acgctcgcca agtctgccgg ccttttgaaa gagaaggggg cgagaagtgt
      601 gcgcgcatta tgtacgcatc ctgttcttag cggtaatgcc tatcagaata tcgagaatag
      661 tgtgcttgac gaactggtcg tatgcgacac gatcccgttg aagcaggcgg gtaagaagat
      721 caaggtgctg agtgtggcag agttatttgc ggtggctatc cggaatgcct atgagaacag
      781 gagcatcacc gggttgttta tccattcgca gctaaggggc aaataagaaa aattaaacaa
      841 aaataaaatg aagacgatta caatcgaagg acaactcagg accgaaattg gcaaacaagc
      901 cacccgccaa cttcgctctg aggaaaaagt gcccggtgta atttacgggg gtgcaaagga
      961 ggtgaatttt tcggctccgg cgactgcgtt caaaacactg gtttatacgc ctgactttca
     1021 gttggccgaa atcaaggtgg acggtagcgc ctacaggtgt atcctgaagg atctgcagtt
     1081 cgacaaggtg accgatgacc tgatccatgc cgatttcctg gaactggtgg aagataagaa
     1141 ggtgattgcg acgatcccga tcaagttcac gggagcggcc aaaggcgtaa aggacggagg
     1201 caagctgatc acgaaaatga aggccctgaa ggtaaagacc tatccgaagt ttttgaaaga
     1261 gaatattgag gtagacctga ccgagcttga gctgaatggc aacgtgagag tggaggatgt
     1321 gaaggcagac aattatgaga tcctcaattc gccgcggatc cctatcgcat cggtcgtgct
     1381 gactcgccag ttgaaacagg aggaagccac tgccacgacc ccagctgcgg gcgccgctcc
     1441 ggctgccgct cctggcgccg ccggtgcaaa acctgctgcc ggcgctcccg ctgccgctcc
     1501 cgccgctgcc gctgcaaaac ctgcgggcgc caagaaataa agtttttctt agtggagacc
     1561 cggttatgat gtaaccgggt ttttgctgta attaggatgt gtgccaacca agtacctgat
     1621 agccgggctc gggaatatcg gtgccgagta tgaaggcacc aggcacaata ttgggtttga
     1681 tatcctggat gcaatggccg ccaaacacca gcaatttttc accgtcgacc ggttagccga
     1741 aagggtggag atcaggtgga agggaaatct gatcgtgggc ataaaaccca cgacgtatat
     1801 gaacctgagc ggccgcgcgg tgaaatactg gatggggaag gacaatataa cgctggacca
     1861 tatcctcgta gtgatggacg aaattgccct gcccctcaac cggttccggc ttcggccagg
     1921 gggcagcgac gccggccata acgggctgac cagcatccag gaggccttgg ggacccagga
     1981 atatgccaga ttgaggtttg ggatcggaag taatttcccc aggggcaggc agatagagta
     2041 tgtcctggga aagtggacgg aacacgaact gccccttgtc cggaagaaga tcgaaaagtc
     2101 ggtggaattg atcgaaagct ttgccagtat tggcattgaa cgaacgatga gcctgtacaa
     2161 taatttggag tttgaactat gatttataaa ggggaatctt tattttcgta taaatattgc
     2221 aaggttccgt aatgaataag tattttgata ttcaatattc ctatgtagca tagatattaa
     2281 ataattgcag gagatgtgct catttaccgg atgcctatct cccgcaaata aaaaccctta
     2341 ttgaatatga aaatattttg tgttggccgt aactatgtgg ctcatgccaa ggagctgaac
     2401 aatgaggttc cagaagagcc gatcatattt atgaagccaa agagcgcgtt gctccaagcg
     2461 catactcctt tttattatcc ggagtttacc aacgagctgc actatgaatg cgagctggtc
     2521 cttcgcatct ccaagaatgg gaagtatatc caggaccgct ttgccagcaa gtattatgat
     2581 gccgttaccg tcggagtcga ttttacagcg cgcgacattc agactgagtt gaaagaaaaa
     2641 ggcctgccct gggaaaaagc caaagcctgg gacaactctg cggccattgg caagtggatt
     2701 ccccttcaga atatcaagaa caagaaggat attaactttt gcctgtacaa gaataaggag
     2761 ctggtgcaac aggggaattc cggcctaatg atcaatagct tcgacaagat cgtggcatat
     2821 atttccaatt atttttcggt caatatcgga gacctggtct ttacgggtac gccggccggg
     2881 gtcggtgaat gcgtcgtggg cgacgagctc gaaggattta tcgaagacga cagtcttttc
     2941 accatagaga tcaagtaacc gatctaccga ccgctccaat gataaccgac gaaattctcc
     3001 tgaaagccta ccggctgatg agggccgcgg ttgagatggc tgccacttac gagaagaatc
     3061 gggcggtctg cagatatgtt cactctacgt cccgcggaca tgaagccgtt caactggcca
     3121 ccgcattcca attgcttccc tgcgattgga tcagcccgta ttatcgcgat gaaagtattc
     3181 tgctggggct tggcttcgag ccttatgacc tgatgctgca actgctggca aaagacgggg
     3241 acatatttac cggcggccgc gaatattact cccatccgaa ttatcgcggc tccaaccatc
     3301 cacggatcat ccatcaaagc agtgcgaccg gaatgcaggc gatcccggcc accggtctcg
     3361 cgcagggact ggcttttctc gaacggatcg gatcaccgct gcttatgcgt ggacccggcg
     3421 gcgtgttacc catcgtcgta tgttcgttgg gggatgccag cgtgacggaa ggggaggtga
     3481 gtgaagcgtt tcagtttgcc gtcttgaaaa agttgccgat catctttctt gtccaggaca
     3541 ataactgggg catcagtgta agagcggacg aatcgcgggc gatggattcc tacgaatatg
     3601 cggccggttt caagggaatg gggcgcttgc gcatcgacgg tagcgacttc cagggcagct
     3661 atgaaacgat gggggctgtg atccgggagg tccggggcgg cggcggccca ttcctcgtac
     3721 aggcatatgt gccgttacta gggcatcata ccagcggcgt gcgccgcgag ttttaccgtg
     3781 atgctgccga ccttgcggag catgcggcaa gggatccggt ccctcgcctt aaggcgcagc
     3841 ttttgttccg gggggtggcc gaggagcagc tcgcaaccat tgaggatgag gtggcgcagt
     3901 cgattgccgc tcaattcagc agggcggttg ccgctcccga gccggtagcc gccaccgttg
     3961 gtgaggatgt gtttgcggcg acgcccgtcc tggaggagcg gggggagcgg acgccggccg
     4021 gacgtgaaaa ggtgccgatg gtcgacgcgg ctttgtttgc gatccgggaa ttgatggaag
//
LOCUS       DATGKE010000116         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_904638, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000116 DATGKE010000000
VERSION     DATGKE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904638"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VKU83_02050"
     CDS             complement(<1..260)
                     /locus_tag="VKU83_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92357.1"
                     /translation="MKTLVRIAFPLILIAFLNADARCQSISGVVNSYYAITAVNSVAN
                     TLTVSNSAGLYTGEPVLIIQMKGATINSGNTSSYGNITAIGDA"
     gene            425..1672
                     /locus_tag="VKU83_02055"
     CDS             425..1672
                     /locus_tag="VKU83_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HLI92358.1"
                     /translation="MQTSRKAAMGFIFVTLLIDVMGFGLIIPVLVDLISKLKGIPANQ
                     ASIYGGYLLTAFAATQFLFSPVIGNLSDRFGRRPVLLFSLFGFGIDYLILAVAPAYQW
                     LFVGRVIAGITGASFTTASAYIADISPDPTERAKNYGLIGAAFGLGFVLGPALGWVLS
                     TMGLRAPFYGAAILCLLNCIYGYFLLPESLPKESRRRFDWKRANPFGSMRFLARHREI
                     GNLAFCFFLIYLAAQAVQSNWTFFTKYRFNWSDGMIYISLIVVGVLIGGVQGGLTRVV
                     NPRLGNEKSIYVGLLLYTFGLVLFAFASQTWMMFAFLVPYCLGGICGPSLQAVISGHV
                     DPNHQGELQGALTSLMSVTTVIGPLIMNGSFTYFTSDKAPFYFPGIHFVIGAACMLMS
                     VVIAYLVLSRERGGAPSVSPPAG"
     gene            1768..2655
                     /locus_tag="VKU83_02060"
     CDS             1768..2655
                     /locus_tag="VKU83_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI92359.1"
                     /translation="MIKITPVLSIVAVGLLFFSACVPMSKFNASQMALSSARYDSAQL
                     AAKVNKLQSDLAQANQTAKEQFDSLNNVIAQEQSQINQLNQQIADLKGKNSDLIAEAA
                     AKQSLLSKSKQDLINQQARLEHLQALMDAQKKAIEEIKKKMSNALVGFKSNELSVSIK
                     NGKVYVSLQENLLFPSGSAVVNPKGKEALGKLAAVLNQNPDITVDIEGHTDSIPIHGK
                     YQDNWDLSLARSASIVRILTTDYKVDPQRVIASGHSQYDPVQPNSTPEGRALNRRTDI
                     ILSPRLDELYRLLQTPDNM"
     gene            complement(2968..3549)
                     /gene="nudK"
                     /locus_tag="VKU83_02065"
     CDS             complement(2968..3549)
                     /gene="nudK"
                     /locus_tag="VKU83_02065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HLI92360.1"
                     /translation="MQEVKILETKVLSDQFYVLRRYQFERTDRSGRRIKQVREVYDRG
                     NGAAILLYNRTSGKVILTRQFRMPTYVNGNPTGMLIECCAGLLDADDAEEAIRRETQE
                     ETGYKVGAVKKIMEAYMSPGSVTEILSLFAAEYSNDMKIGDGGGLEDESEDIEVLEIP
                     YQRALEMVRTGEIKDAKTILLLQYAELNRIMVG"
     gene            complement(3581..>3787)
                     /locus_tag="VKU83_02070"
     CDS             complement(3581..>3787)
                     /locus_tag="VKU83_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92361.1"
                     /translation="GHTHGAQFGVETDKFRWSPVEYRYLDWAGLAREGNRYLYVNRGF
                     GFLAFSGRLGIWPEITVFTLRKTV"
BASE COUNT          876 a         1082 c          904 g          925 t
ORIGIN      
        1 gcgtctccga tagcggtgat attgccgtaa gaggaagtat ttccggagtt gattgtcgca
       61 cccttcattt ggatgatcag cacgggctcg ccagtgtaga gtccggcgct gttgctaacg
      121 gtcaaggtat tggcgacgga attgaccgcg gtgatggcat aataggaatt gacaacgccc
      181 gaaatggatt ggcaacgggc gtcggcattc aagaaagcaa tgaggatcaa tggaaaagcg
      241 atccttacaa gggttttcat acatattggt tctcatgcta aaaatacagc atatatttta
      301 tacttgcaac acagcgatca caatgctaca cattgatacc cagtcattag tgtcatactt
      361 taaaaaagac cggggcttcc ggaaaatgaa gtaagtttgt ccacacacca gaaagaggcc
      421 gctcatgcag acatccagga aagccgcgat gggttttata ttcgtcacgc tcctcatcga
      481 cgtaatggga tttggtttga tcatccccgt cctggtcgat ctgatcagca aactaaaagg
      541 cattcccgct aatcaggcaa gtatctatgg aggatacctg ctaactgcct tcgccgcgac
      601 tcagttcctt ttttccccgg taatcggcaa cctgagcgac cgctttggcc gccggccggt
      661 tctccttttc tccctgtttg gatttggcat tgattacctt atcctcgccg tcgcaccggc
      721 gtatcaatgg ctgtttgtcg gccgggtcat cgccggcatc accggcgcca gttttaccac
      781 cgcgagcgcc tatatcgcgg acatcagccc tgatccgacc gaaagagcaa aaaactatgg
      841 actgatcggc gccgcattcg gtctgggctt tgtcctcggc cccgctctcg gctgggtact
      901 gtccacgatg ggcttgcggg cacctttcta tggcgccgcg atcctttgcc tgctcaactg
      961 catctacggc tatttcctgc tgcctgaatc gctcccgaaa gagagccggc gccgcttcga
     1021 ttggaagcgc gcgaatccgt tcggttccat gaggttcctg gcccgccacc gcgagatcgg
     1081 caatctcgcc ttctgctttt ttctaatcta ccttgccgct caggcagtcc agtcaaactg
     1141 gactttcttt acgaagtatc gatttaactg gagcgacggc atgatctata tttccctgat
     1201 cgttgtgggc gtcctgatcg gtggcgtaca aggtgggctg accagggtcg tcaatccaag
     1261 gctgggaaat gaaaagagca tttatgtcgg tttgctttta tacaccttcg gtcttgttct
     1321 gttcgccttt gccagccaaa cctggatgat gttcgcgttc cttgtcccct attgcctcgg
     1381 cggaatctgc ggcccctcct tgcaagcagt catttccggc catgtcgatc ccaaccacca
     1441 gggtgaattg cagggcgccc tgacgagcct gatgagcgtg acaaccgtta tcggtcccct
     1501 gatcatgaac gggagcttta cctatttcac cagcgacaag gcgccgttct atttcccggg
     1561 catccacttt gttattggcg ccgcctgcat gttaatgagt gtcgtcattg cctatcttgt
     1621 cctaagccgc gaacgcggcg gcgctccgtc agtatcgccg cccgccggct agcagcggtc
     1681 atcagccccg ctctaatccc gttctaataa gattttaagc cgcttctaaa cccaagtcga
     1741 tatttttgcg ctcaaataca tagtattatg ataaaaatca cccctgtcct gtctattgta
     1801 gctgtggggc ttctcttctt cagcgcttgt gttcctatga gcaaattcaa tgcgtcccag
     1861 atggccttgt cttcggcacg ctatgacagc gcccagctgg ctgcgaaagt caataaactg
     1921 caatccgacc ttgcccaggc caatcagacg gcaaaggagc aattcgactc cctgaacaat
     1981 gtgatcgccc aggaacaaag tcagatcaat cagctgaacc agcagattgc cgatctgaaa
     2041 gggaagaata gcgatctcat cgccgaggcc gccgcgaaac aatcgctgct cagcaagagc
     2101 aaacaggacc tgatcaacca gcaggcccgg ctggagcacc tgcaggcgct tatggatgcc
     2161 cagaaaaaag ccattgaaga gatcaaaaag aaaatgagca acgccctcgt tgggtttaaa
     2221 tccaatgaac tctcggtgtc tataaaaaac gggaaagttt atgtcagtct gcaggaaaac
     2281 ctgctcttcc cgtcgggtag cgccgtcgtc aatccgaagg gtaaagaagc cctgggcaaa
     2341 ttggccgcgg ttttaaacca gaaccccgat atcacggtcg acatcgaagg acatacggac
     2401 tccattccca tccatggtaa atatcaggat aactgggatc tcagcctggc gcgatccgct
     2461 tcaatcgtcc gaatcctgac aacggactat aaagttgacc cccagcgcgt catcgcatcg
     2521 ggccacagcc agtatgatcc ggtgcagccg aatagcacgc ccgaaggacg ggccctcaac
     2581 agaaggaccg atatcattct ttcgccgcgg ctggacgagc tttaccgcct cctgcagaca
     2641 cccgataata tgtgaccttc ataccaggtc ctgcggcggg gcttctgctt taacttcttg
     2701 gctgcctggg gcaaaccgac gcgcccgggc agccaatcca ccttattaat ccgattgcag
     2761 ggagcacaaa ccggctttgc cggccgcccg ccagggtcga ccaaacaata attatcgaaa
     2821 aaagttgatt gatagccggc aggcaattct ttaccagatc aaaataatcc tgtatcttag
     2881 ccccagttta gaacaaaggt ataatggtta acaagccgcg gcttttccaa gccggggctt
     2941 ttttattttc ggagcagttc tctttcctta gccaaccatt atcctattta attccgcata
     3001 ctgcagcaag aggatggtct ttgcatcttt gatctcgcct gtcctaacca tctccaatgc
     3061 ccgctgataa ggaatttcca gcacctcgat atcttcgctt tcgtcctcca ggccgccacc
     3121 atcgccgatc ttcatgtcat tcgaatactc agccgcaaac aggctaagga tctcggtcac
     3181 cgaacccggc gacatatacg cctccatgat ctttttcacc gcgccgactt tataacctgt
     3241 ctcttcctgg gtctctcgcc ggattgcctc ctccgcatca tccgcgtcca aaaggccggc
     3301 acagcactcg atcagcattc cggtggggtt cccgttcaca taagtaggca ttctgaattg
     3361 ccgcgtcagg attactttac ccgaggtcct attgtagagc aaaatagcag cgccatttcc
     3421 gcggtcgtaa acctccctga cctgttttat ccggcggccg cttcggtcgg ttcgctcaaa
     3481 ctggtaccgc cggagaacgt agaattggtc tgacaaaact ttggtttcca aaattttaac
     3541 ttcctgcata tactggcatg attgttattg atcgcatgag tcaaacggtc ttcctcagag
     3601 tgaaaacggt tatctcgggc caaattccca acctgcctga aaaagccagg aacccgaatc
     3661 cccggttgac gtacaaatag cggttgcctt ccctggccag gcctgcccaa tccaaatacc
     3721 ggtattccac cgggctccac cggaacttat ccgtttcgac gccaaattgg gctccatggg
     3781 tgtgccc
//
LOCUS       DATGKE010000117         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_821145, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000117 DATGKE010000000
VERSION     DATGKE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821145"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VKU83_02075"
     CDS             <1..467
                     /locus_tag="VKU83_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLI92362.1"
                     /translation="SAYVVKRDQPGWTTFSIPRAFWYSTAVILVSSLTIQLSLKAFKD
                     REMMRYRNFLTLTAVLGILFVFLQWTGFRQIWHTGITLHGSGGGQFLYVIAGLHAVHV
                     LGGIVALLIIVARAFATRTRSYNSVPVEIMSTYWHFVDLLWIYLFIFFMAIN"
     gene            510..1184
                     /locus_tag="VKU83_02080"
     CDS             510..1184
                     /locus_tag="VKU83_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLI92363.1"
                     /translation="MAQAIPAGTKWWSGGKSPFNVEYGKLMMWYFLMSDAFTFGAFLI
                     SYGTIRVSSNSWPDPNEVFRAFPGMGEAHLPLVFVSLMTFILIMSSVTMVLAVAAGHQ
                     GDRKKVTFYMILTILGGLAFLGCQAWEWTHLHGEGAWWGRNPFPNVDGSKASTNFTNF
                     FFTITGFHGFHVFSGVCINVIMLIKNLRGDFEKRGHYLMIEKAGLYWHFVDLVWVFVF
                     TCFYLL"
     gene            1218..1646
                     /locus_tag="VKU83_02085"
     CDS             1218..1646
                     /locus_tag="VKU83_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HLI92364.1"
                     /translation="MSSIENTSRDNAHVKFDTRPIWRTFWVLLVITLFEITLATVHMK
                     LVEHGHEFPPKMVLNGVYLILTCAKAFYIIAEFMHLRHEIKNMIMTIAVPATLFIWFI
                     LAFTLDGASYNTLRNRYDRHFADQNKVKVEQPAQKPNSPE"
     gene            1675..2490
                     /locus_tag="VKU83_02090"
     CDS             1675..2490
                     /locus_tag="VKU83_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLI92365.1"
                     /translation="MSKKSWLAICLAVIVPLVFYGIANTYGVVMPPRYFPDSVVTKMK
                     DGKEVTDTIWHKVANIRLVNQLGDTVSLDSLRGKVLCIDFFFTHCASICPILTRNMRH
                     MQDALKSPDKIRQVDAADTNFVQFVSLTVDPAHDSPQVLKRYADKYGVNSDVWWMLTG
                     PKKTIYDFALKELKLGLQDSVNVDSNFVHSDLIALLDRDRVIRGYYDGTDTTALKRLG
                     NDMVFIMLEKDKNRRSTVFDELKPMRIPIAIILLGTVVGVIYFSRKRKGTEYS"
BASE COUNT          586 a          669 c          688 g          619 t
ORIGIN      
        1 ccagcgcata tgtggtaaaa cgggaccagc cgggatggac cactttctcc attccgcggg
       61 cgttctggta ctccacagcg gtgatcctgg tgagcagcct caccatccag ctgtcgctca
      121 aggcattcaa ggacagggag atgatgcggt accggaattt cctgacactg acggccgtgc
      181 tggggatact gttcgtgttc ctgcaatgga ccggattccg gcagatctgg catacgggga
      241 taaccttaca cgggtccggc gggggccagt tcctgtatgt gatagccggg ctgcatgcgg
      301 tgcatgtgct gggtgggatc gttgccttgc tgatcatcgt tgccagggca ttcgcgacga
      361 ggacccggag ctataatagt gtacctgtgg agatcatgag cacctattgg cattttgtcg
      421 atctgctctg gatctacctg ttcatattct ttatggcgat aaactaagat tattattaat
      481 aaaatatatt tacaaaacaa accagcaata tggcacaagc aataccggca gggacgaaat
      541 ggtggagtgg cgggaaaagc cccttcaatg tggaatatgg caaattgatg atgtggtatt
      601 ttttgatgag cgatgccttc accttcggcg ctttcctgat ctcttacggt actatccggg
      661 tgtccagtaa cagttggccc gaccctaatg aagtgttccg cgctttcccc ggtatgggcg
      721 aggcgcacct ccccctggta ttcgtgagcc tgatgacctt tatcctcatt atgagctcag
      781 tgacgatggt cctggctgtg gcagcaggcc accagggcga ccggaagaaa gtgacctttt
      841 acatgatcct gacgatcctc ggcggtctgg ccttcctggg ctgccaggcc tgggaatgga
      901 cgcacctgca cggtgaaggc gcctggtggg gccggaatcc cttccccaat gtcgacggtt
      961 ccaaagcatc taccaatttc acgaactttt tcttcaccat aacgggtttc catggcttcc
     1021 acgtcttttc cggcgtctgc atcaatgtga tcatgctcat caagaacctg cgcggggatt
     1081 tcgaaaagag aggccattac ctgatgatcg aaaaggccgg gctgtactgg cacttcgttg
     1141 acctggtatg ggtatttgtg ttcacctgtt tttatctgct gtaacaaagg aatttaaaag
     1201 acaacaaaat aatgactatg tcaagcatcg agaataccag ccgcgacaac gctcacgtta
     1261 agttcgacac gaggccgatc tggcgtacct tttgggtgct gctggtgatc acgctgttcg
     1321 agatcacgct ggctaccgtc catatgaagc tggtggaaca tggccatgag ttcccgccga
     1381 agatggtcct caacggggta tatctgatcc tgacctgcgc gaaggctttc tatatcatcg
     1441 ccgagttcat gcacctccgg cacgagatca agaatatgat catgacgatc gcggtgccgg
     1501 ccaccctgtt tatctggttc atcctcgcct ttacgctcga cggggcttcc tataacaccc
     1561 tgcgcaaccg ctacgaccgg cactttgccg accaaaataa agtgaaggtc gaacagcctg
     1621 cgcagaagcc caattctccc gagtagggtt aagcgggttc aaaagcaatg aaaagtgagt
     1681 aagaaatcgt ggctggctat ctgtctcgcg gtgatcgtac cgctggtgtt ttacgggatc
     1741 gcgaacacct acggcgtcgt tatgccgccg cgttattttc ccgattccgt cgtgaccaaa
     1801 atgaaagacg ggaaggaagt caccgacacc atctggcata aggtcgccaa tatccggctg
     1861 gtcaaccagc tcggcgacac cgtttcgctg gattcccttc gtggcaaggt cctctgcatc
     1921 gacttcttct ttacgcattg cgcctccatt tgcccgatcc tgacgcgcaa catgcgccat
     1981 atgcaggacg cgctcaaatc tccggacaag atcaggcagg tagacgccgc cgacaccaat
     2041 tttgttcagt ttgtctcgct cacggtggat ccggcccacg actccccgca ggtgctgaag
     2101 cgctatgccg ataaatatgg ggtgaactcg gatgtctggt ggatgcttac cgggcctaaa
     2161 aagacgatct acgattttgc gctcaaggaa ctcaaactcg ggttgcagga cagtgtcaat
     2221 gtcgattcga acttcgtcca ctccgacctg atcgcattgc tggacagaga ccgggtcata
     2281 agaggctatt atgacggcac cgatacaact gcgctcaaga ggctggggaa cgatatggtc
     2341 tttatcatgc tcgagaaaga caagaaccgg agatccacgg ttttcgacga gctgaagccg
     2401 atgcgcattc cgatcgcgat catcctgttg ggtacggtcg tgggtgttat ctacttcagc
     2461 cggaaaagga aaggaacaga gtacagttaa cccatgacca tggcaggatc tgttgcacag
     2521 cgcagtttta caagggcgat cattttcgct tcgattgcca tt
//
LOCUS       DATGKE010000118         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1005759, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000118 DATGKE010000000
VERSION     DATGKE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005759"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2492)
                     /locus_tag="VKU83_02095"
     CDS             complement(<1..>2492)
                     /locus_tag="VKU83_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HLI92366.1"
                     /translation="ATYKYYIDFAADNHLRYILLDEGWSDDADLMKVVPDIDLPAIIG
                     YARERNVGVWLWSGALAMGRQMDEVMHHYSEMGIKGFKVDFMNRDDQQMVRFYYRAAK
                     TAAACHLMLDFHGAYKPTGLQRTYPNVLNFEGVRGMENEKWSNDDFPSYDVSIPFIRM
                     LAGPLDYTPGAMKNYNKANFRPIFSEPMSQGTRCHQLAMYIVYEAPFSMLSDNPTSYR
                     REPESVQFIAGVPSFFDETRSLDGEVGQYVMLARRKAGDWWVGAMTNWTPRDLNVDLS
                     FLPSGNYRAEIMRDGINADRDGTDYRKETVEVTRESKLAIHLAPGGGWVAKIHPEGEG
                     AAAGSATDGAAAGTGMKGGAAVPPPAIDVNQVGFYPRAPKMAIVAGGPDAAENFYLVS
                     MNGGDTVFRGLLGPATPSANSSLSTRLADFSAFRSPGLYSVVVPGLGRSHSFRIADKV
                     LFPVARAALKGFYYIRSGTALTPAYAGKWSRVAGHPDTEVLVHPSAATASRPAGTVIR
                     TPGGWYDAGDYNKYVVNSGITMGTLLDAYEDFPGFFDTLHTNIPPVPGVPDLLNEVLY
                     NLRWMLTMQDPGDGGVYHKCTNAAFDGMVMPDITRAPRYVVQKGTAATLDFAAVMAQA
                     ARMLRSFRRSLPSLSDSCLRAAEYAWQWALQHPAVIYDQDQLDRTFTPAITTGAYGDR
                     HFNDEWFWAASELLVTTGNDQYGQVVREHAGDTMILPSWSNVQMMGDYTLLRYRKSLP
                     ASWEPLIRSVQGRVLRMADSWLAQTGANAFHSVMGESRRDFVWGSNSVAANQGMLLVA
                     AWLQTGDGRYLAPAVANMDYLLGMNATGYCFI"
BASE COUNT          516 a          768 c          720 g          488 t
ORIGIN      
        1 aataaaacaa tatccggtag cgttcatgcc cagcaggtaa tccatattcg ccaccgcggg
       61 cgccaggtat ctgccgtcgc cggtttgaag ccaggccgcc accagcagca ttccctggtt
      121 ggcggcaacc gaattgctcc cccagacgaa atcgcgccgc gactcgccca tcacggaatg
      181 aaaagcgtta gcgccggtct gggccagcca gctgtccgcc atgcgcaaaa cccgcccctg
      241 aactgaccgg atcaggggtt cccaggatgc cggaagggat ttgcgatagc gaagcaaagt
      301 ataatcaccc atcatttgca cattggacca ggagggaagg atcatggtat cacccgcatg
      361 ttcgcggaca acctggccat attggtcgtt cccggtggta accaaaagct ccgaggcggc
      421 ccagaaccat tcgtcattaa agtggcggtc gccataggcg ccggtcgtga tagcgggagt
      481 gaaggttctg tcgagctgat cctggtcgta aatcaccgcg ggatgctgca gcgcccattg
      541 ccatgcatat tccgccgcac gcagacagct gtcggaaagc gagggcagcg aacgtcggaa
      601 gctacgcagc atccgcgcgg cctgggccat taccgccgcg aagtccagcg tggcggcggt
      661 gcctttttgc acaacatatc ggggcgcccg ggtgatgtcg ggcatgacca tgccgtcgaa
      721 ggccgcgttc gtgcatttgt gatagacgcc gccgtctccg ggatcttgca tggtcagcat
      781 ccaccgcaaa ttataaagga cttcgttgag aaggtccggt acgccgggca ccggcgggat
      841 gttcgtgtgc agcgtgtcaa aaaatccggg aaaatcctca taagcgtcca gcaatgtgcc
      901 catggtgatc ccggagttga cgacgtattt attatagtca cccgcgtcat accagccgcc
      961 cggcgtacgg atgacggtgc ccgcgggccg cgatgccgtt gcggccgacg ggtgaacgag
     1021 cacctcggta tccgggtgcc cggcaaccct gctccatttt ccggcgtagg ccggcgtcag
     1081 ggccgtccct gaacggatgt aatagaatcc ctttaaggct gcccttgcca cggggaaaag
     1141 gaccttgtcc gctatccgga aggaatggga gcggccgagc ccggggacca cgacgctata
     1201 gaggccgggg ctccggaaag cgctgaaatc cgcaagccgg gttgatagtg aagaattggc
     1261 ggacggggtt gcggggccaa gcagtcccct gaagacggta tcacccccat tcatcgatac
     1321 gagataaaaa ttttcggcgg catccggacc gccggcgacg atggccattt ttggcgcacg
     1381 cggatagaag ccgacctggt tcacgtcgat tgccggcggt ggaaccgccg cgccgccttt
     1441 cattccggtg cccgcggcgg ccccgtccgt tgcagacccc gcggcggccc cctccccttc
     1501 cggatggatc ttcgccaccc agccgccgcc cggggccaga tggatcgcca gtttgctttc
     1561 gcgtgtcacc tccaccgtct cctttcgata gtccgttcca tcccggtccg cgttaatccc
     1621 atcgcgcatg atctccgccc gatagttccc cgaaggaaga aaggaaaggt cgacgttcag
     1681 gtccctcggc gtccagttgg tcatcgcccc gacccaccag tcacccgctt tccgccgcgc
     1741 caacatgacg tattggccta cctctccgtc cagcgaccgg gtctcgtcaa agaatgaggg
     1801 cacacctgca atgaattgca cactttccgg ctcccgccgg taactggtag ggttgtccga
     1861 aagcatgctg aaaggcgcct cgtatacgat gtacatcgct aactgatgac aacgcgtacc
     1921 ctggctcatc ggctcgctga aaatcggcct gaaattcgcc ttgttgtagt tcttcattgc
     1981 gcccggcgta taatccaggg gccctgccag catccggata aagggaatgg aaacgtcata
     2041 ggaggggaaa tcgtcgtttg accatttttc gttctccatg ccgcggacac cctcgaaatt
     2101 caggacattg ggataggtcc gctgcagtcc ggttggctta taggcgccgt ggaaatccag
     2161 catcaggtga cacgcggcgg ccgtttttgc cgcacggtaa tagaaacgca ccatctgctg
     2221 gtcgtcgcgg ttcataaaat cgaccttgaa gcccttgata cccatctcgg aatagtggtg
     2281 catcacctcg tccatctggc gtcccatggc cagcgctccc gaccagagcc agacgccgac
     2341 attccgttcc cgcgcatacc cgatgatcgc cgggaggtcg atatcgggca cgaccttcat
     2401 caaatccgcg tcatcggacc agccttcgtc caacaggatg taacggagat gattatccgc
     2461 ggcaaaatcg atataatact tgtacgtggc gg
//
LOCUS       DATGKE010000119         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_903100, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000119 DATGKE010000000
VERSION     DATGKE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903100"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VKU83_02100"
     CDS             <1..814
                     /locus_tag="VKU83_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI92367.1"
                     /translation="RQEGRPVRLMDRRAAAETSAAVFTEGLLGGLYCDDELLVDPREA
                     IAALPGWLAGELGIAFHWGKCISYISDQTVYIGNDEEYEADLIFICSGADFETLYPEH
                     FRGQPLTKCKLQMMRLAVQPENWRIGPALCGGLSLIHYNAFKAAPSLPALRRRYQDEM
                     ADYLEWGIHVMVAQNGRGELTVGDSHEYGLTHDPFDKKCINARVLAYLRRFAAVRDWT
                     VIETWNGVYAKLTDGEADLFFSPEPYVYVINGVGGAGMTLSFGLAEELVAGL"
     gene            888..1526
                     /locus_tag="VKU83_02105"
     CDS             888..1526
                     /locus_tag="VKU83_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLI92368.1"
                     /translation="MKSLKAILADNRYFFYCYLLFVLVGLIILASLGKAASFIDLNPW
                     HSKLMDTCFMGVTFLGDGRFAILFCLIYLLMRRWSRACQLIAAFLCSALVAQILKNVF
                     SMPRPKQFFPAGQYAYFIDGVTHIGFASFPSGHSTSVFALATLLAIFDSNKRGNVLYL
                     LAAVAVGYSRIYLGQHFLPDVLAGSALGATTAVLIHWLFSTKLRSVRAFARG"
     gene            1606..2355
                     /locus_tag="VKU83_02110"
     CDS             1606..2355
                     /locus_tag="VKU83_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI92369.1"
                     /translation="MIEGKKLVIVLPAYNAELTLFKTYSEIPFEIVDEVVLVDDNSSD
                     RTVAVARELGIRHIIRHDKNRGYGGNQKTCYDKALELGADIVIMLHPDYQYTPKLIPS
                     MAWLIANDVYQVVLGSRTLGRGALHGGMPLYKYVFNRALTFFENLMLRQKLAEYHTGY
                     RAFSREILTRIDYHINSEDFVFDNQILSQIIYLGYEIAEITCPTRYFEEASSINFSRS
                     VTYGLGVLKVSVVHRLCKWGLMKRDMYKPVC"
     gene            2355..>3157
                     /locus_tag="VKU83_02115"
     CDS             2355..>3157
                     /locus_tag="VKU83_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLI92370.1"
                     /translation="MHINKTTTILLITALAALLFIPFLGHVHLFDWDEINFAECSREM
                     IKLDDYSRVYVDFKPFWEKPPMFFWMQSSAMRVFGINEFAARFPNAICGIVTLITIFL
                     CGSRIYDRKLGLLWALAFGGSLFPNMYFKSGIIDPWFNLFTFLSLYHFILYHWKRNGY
                     DKPGINKSKAYYVVMSGVFMGLAVLTKGQVALIVFLLALGVYFCYNRFRFYFNWWHAL
                     LFLLVAFAVTFTWYGYETIKRSDWFIVQFLKYQWRLFTTHDADQKGFFG"
BASE COUNT          603 a          888 c          884 g          782 t
ORIGIN      
        1 ccggcaggaa gggcgtccgg tccggctgat ggaccgccgt gctgcggcgg agacctcggc
       61 tgcggtgttc accgaaggtc tgctcggcgg gctctactgt gatgacgaac tgctcgtcga
      121 cccgcgcgaa gcgatcgcgg cgctcccggg ctggctggca ggggagctgg gcattgcttt
      181 tcactggggg aaatgcatct cgtatatctc cgatcagacc gtgtatatcg ggaacgacga
      241 agaatacgaa gccgacctca tctttatctg cagcggggcg gattttgaaa ccctgtaccc
      301 ggaacatttc cgcgggcagc ccctcaccaa atgcaaactt cagatgatgc ggctggcggt
      361 gcaaccggaa aactggcgga tcggcccggc actatgcggc ggactttcgc ttatccacta
      421 taacgctttc aaggccgcgc cgtcgctgcc cgccttgcgg cgccgttacc aggacgagat
      481 ggctgactac ctggaatggg gcatccatgt catggtcgcc cagaacggca ggggcgagct
      541 gacggtaggc gactcgcacg aatacgggct cacgcatgat cccttcgaca aaaaatgcat
      601 caatgcgagg gtccttgcgt acctgcgcag atttgccgcg gtcagggact ggaccgtcat
      661 cgagacgtgg aatggcgtct acgcgaagct gacggacggg gaggccgatc tctttttctc
      721 gccggagccc tacgtttacg tgatcaacgg agtgggcggc gccggaatga ccttatcttt
      781 cggcctggcg gaggagcttg tggccggact ctgaacggca ggggccgccg ggtccgcgag
      841 gccgctaccc gtgagcgtgc ttgccgcgga ttccttagtt ttacgggatg aagagcctca
      901 aggcgatact ggccgacaac cgctattttt tttattgcta cctccttttt gttctcgtgg
      961 gcctcattat tctggcaagt cttggcaagg ccgccagttt tatcgatctt aacccgtggc
     1021 atagcaagct catggatacg tgttttatgg gcgtcacctt tctcggtgac ggccggtttg
     1081 cgatcctgtt ttgcctgatc tatctgctca tgcgacgatg gtcccgggcc tgccagctga
     1141 tcgctgcttt tctttgttcc gcgctggtcg cccagatcct gaagaatgtc ttttccatgc
     1201 cgcgtccaaa gcaattcttt cccgccggcc aatacgctta ttttatcgat ggcgtgacgc
     1261 atatcgggtt tgcgagcttt ccgtccgggc actcgacatc cgttttcgcg ctggccaccc
     1321 tgctggcgat ctttgacagc aataaaaggg ggaatgtact ttacctgctg gcagccgtgg
     1381 ccgtgggtta ctcgcgcatt tatctcgggc agcactttct gccggacgtg ctggccggct
     1441 cggcgctggg ggcaactacc gccgtgctta ttcactggct tttttccacg aagctgcgct
     1501 ccgtccgggc tttcgcgcgc ggatagcagg tttgccgcgc agccctggcg aaatttcacc
     1561 gaatgcccgg tttgacgtag gtttgccctc aattttcgat atcgtatgat tgaaggtaaa
     1621 aagctggtca ttgtccttcc cgcctataat gcagaactta ccctgtttaa gacctattcc
     1681 gagatcccgt tcgagatcgt ggacgaggtc gtgctggtgg atgataacag cagcgaccgg
     1741 acggtagccg tggcccgcga actgggcatc cggcatatta tccggcacga taagaaccgg
     1801 ggttacggcg gcaaccagaa aacctgttat gacaaggcgc tggagctggg cgccgacatt
     1861 gtgatcatgc tccatcccga ctatcaatat acgcccaaac tgatcccttc catggcctgg
     1921 ctgatcgcaa atgacgttta ccaggtcgtt ctcggctccc ggacgcttgg ccggggcgcg
     1981 cttcacggcg gcatgccgtt atataagtat gtattcaacc gtgccctgac cttcttcgag
     2041 aatctaatgc tccgtcagaa gctggcggaa tatcataccg gttaccgcgc gttttcccgc
     2101 gagatcctca cccggatcga ctatcacatc aattcggagg attttgtttt tgataatcaa
     2161 atactgtccc agatcatcta tttaggctac gagattgccg agatcacctg ccccacccgg
     2221 tatttcgaag aggcctccag catcaatttc agccggagcg tcacctacgg cctgggagtg
     2281 ctgaaggtca gcgtggtcca ccggctctgc aaatgggggc tgatgaagcg ggacatgtat
     2341 aaacccgtct gctgatgcat attaacaaga ccaccaccat cctgctgatc actgcgctgg
     2401 cagcgctgct gttcattccg ttcctgggcc acgtccattt gttcgactgg gacgagatca
     2461 attttgccga gtgttcgcgc gaaatgatca aactggatga ctacagccgg gtctatgtcg
     2521 attttaagcc gttttgggag aaacccccga tgttcttctg gatgcaaagc agcgccatgc
     2581 gcgtgttcgg catcaacgaa tttgccgccc gttttcccaa cgcgatctgc ggcatcgtca
     2641 ccctcatcac catttttctt tgcgggtccc ggatctatga ccggaaactg ggcctgttgt
     2701 gggccctggc gttcgggggc tccctgttcc cgaacatgta tttcaagtcg gggataatcg
     2761 acccctggtt caacctgttt acgttccttt ccctatacca tttcattctc taccattgga
     2821 aaaggaatgg ttacgacaag cccgggatca acaaaagcaa ggcttattat gtggtcatgt
     2881 ccggggtttt tatgggcctt gccgtgctga ccaagggcca ggtcgccctg atcgtcttcc
     2941 tgctcgcgct cggggtttat ttttgctaca accgcttccg cttctatttc aattggtggc
     3001 atgccttgct tttcctcctg gtcgcctttg cggtaacgtt tacctggtac gggtacgaga
     3061 cgataaaaag gagcgattgg ttcatcgtgc aattcctgaa ataccagtgg cggctgttca
     3121 cgacgcatga cgcggatcag aagggattct tcggcta
//
LOCUS       DATGKE010000120         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1421992, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000120 DATGKE010000000
VERSION     DATGKE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421992"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VKU83_02120"
     CDS             complement(<1..451)
                     /locus_tag="VKU83_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92371.1"
                     /translation="MLRNYWRIAWRFLVRNKLYTCIHILGLALGICGCLVLFLVTRYE
                     FSFDRHWKDGDRIYRIVGEIHRPDGLEMFTNSPFDDVAGFETQIPGFEAKAAMFPYDG
                     KISVPQGAGPAKHFDNHIGDQYQSAAVITVPDYFRVFPYRWLAGNAAV"
     gene            complement(503..>2857)
                     /locus_tag="VKU83_02125"
     CDS             complement(503..>2857)
                     /locus_tag="VKU83_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLI92372.1"
                     /translation="RVEKLFDIQKRVANQCLIEAKKKFAEGNDDPKDGGLALMRAYRG
                     LPKSGPLIKFLSEPGIKVKLQKTENYYLADQSKEMPKVDAELYFTIEEKNNSVDLTDK
                     GIQMITREGEDPEFFILPDISIKLAEIEQSPASNEEKLQQKEALLNEYAVKADRIHTV
                     QQLLKAYTLFDKDVEYVVLDGQVKIVDEQTGRILDGRRYSDGLHQAIEAKENVKIEAA
                     TQTYATVTLQNYFRMYHKLAGMTGTAETEAAELWDIYKLDVVTIPTNVPAIRIDNQDL
                     VFKTKREKYKAVIDEIERLRAAGRPSLVGTTSVEVSELLSKMLQVKKIPHNVLNAKQH
                     AREAQVVAEAGLAGAVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQL
                     RGRSARQGDPGTSQFYVSLEDDLMRMFGSDRIASLMDRMGYKEGEVIQHSMVTKSIER
                     AQKKVEENNFGIRKRLLEYDDVMNKQRNVVYKKRNHALFGDRLALDLDNAFYIVAESL
                     INSFREQDDYEGFKLAAIVNFGTDTKIGQEEFLKGDTGELAEKLYQEATQNYERKLDE
                     LRKQAVPVFSNIRATQGSHIENVVVPFSDGKKRMNVIANLQKTLNTDGRELTNAVERQ
                     ITLAIIDEAWKEHLRAMDDLKQSVQTAYLEQKDPLVIYKVEAFELFRRMDSDVNRDII
                     GFLCHSGIHAGSGHQQQAIREGREQRTDMSKLRVRKDELAEAGVVSGGAPGRVPTEGG
                     GDYHDPSAPPAKREPVKVGPKIGRNDPCPCGSGKKYKNCHGREA"
BASE COUNT          497 a          899 c          763 g          698 t
ORIGIN      
        1 gcaccgccgc atttcccgcc aaccaccgat agggaaacac gcggaaataa tcgggaacgg
       61 ttataaccgc cgcgctctgg tattgatcgc cgatgtggtt gtcgaaatgc ttcgccggac
      121 ccgccccctg cggaacgctg atctttccgt cgtaggggaa catcgccgcc tttgcctcga
      181 aacccggtat ctgggtttca aaccctgcga catcgtcaaa ggggctattc gtaaacatct
      241 ccagaccgtc aggccgatgg atctccccga cgatccggta gatccggtcg ccgtccttcc
      301 aatggcggtc aaaactaaat tcataccggg tgacaagaaa gagcaccagg catccgcaga
      361 ttcccagcgc gaggcccaga atatggatgc aggtgtagag cttgttgcga acgaggaatc
      421 gccaggcgat ccgccagtag tttctgagca tcatttatgg tttcccttga ccagatgcgc
      481 aatgggtgct tttccataat ccttaagcct cccggccgtg gcagttcttg tatttcttcc
      541 cgctgccgca ggggcaaggg tcgttccgcc cgattttcgg ccccaccttg accggctccc
      601 gcttggcagg gggcgccgat ggatcatggt aatcgccgcc gccttccgta ggcaccctgc
      661 caggcgcccc gcccgagaca acgccggctt cggccagctc atccttgcgg acccggagct
      721 tactcatatc ggtccgctgc tcgcgtccct cgcggatcgc ctgctgctga tgaccggagc
      781 cggcgtggat ccccgaatgg caaaggaaac cgatgatgtc gcggttgaca tcgctgtcca
      841 tccgccgaaa gagttcgaat gcctctacct tgtagatgac cagcgggtcc ttctgctcga
      901 gataggccgt ctgaacgctt tgtttcagat catccatggc gcgtagatgc tctttccagg
      961 cctcatcgat gatcgccagc gtgatctggc gttccacggc attggtgagc tcgcggccgt
     1021 cggtattcaa ggtcttctgc aggttagcga tcacattcat ccgcttcttg ccgtcggaga
     1081 aaggcacgac gacattctcg atatggctgc cctgggtcgc gcggatattg ctgaacacgg
     1141 gaaccgcctg tttgcgcagc tcgtccagtt tccgctcata gttttgtgtg gcctcctggt
     1201 aaagcttctc ggccagctcc cccgtatccc ccttgaggaa ttcttcctgc ccgatcttcg
     1261 tgtccgtccc gaaattaacg atcgccgcga gtttgaatcc ctcatagtcg tcctgttcgc
     1321 ggaacgaatt gatgaggctt tcggcaacga tatagaatgc gttgtcaagg tccagcgcca
     1381 gccggtcccc gaatagcgcg tggttgcgct tcttgtacac gacatttcgc tgcttgttca
     1441 tgacgtcatc gtattcgagc agccgcttgc ggatcccaaa gttgttctct tcgaccttct
     1501 tctgcgcccg ctcgatgctt ttcgtcacca tgctatgctg gatgacctcc ccttccttat
     1561 aacccatccg gtccatcagc gacgcgatcc ggtcgctgcc gaacatccgc atcaggtcat
     1621 cttccagcga tacgtagaac tgcgaagtgc ccgggtctcc ctgccgggcg gaacgaccgc
     1681 gcaactgccg gtccacgcgg cggctttcat gccgttccgt gccgatgatc gcaaggccgc
     1741 cggcctcttt cacgcctggc ccaagcttaa tatcggtacc gcggccggcc atattcgtcg
     1801 cgatcgtcac tgccccggcc agcccggctt cagcgacaac ctgcgcttcc cgcgcgtgct
     1861 gtttcgcatt taaaacgtta tgagggatct tcttcacctg cagcatcttg ctcagcagct
     1921 cgctgacctc gaccgaagtg gtgccgacca gtgagggccg gccggccgcc cgcaaccgct
     1981 cgatctcatc gatgacggcc ttgtattttt cgcgcttggt cttgaagacc aggtcctggt
     2041 tgtcgatacg gatcgccggg acgttcgtag ggatcgtcac cacatccagc ttgtagatat
     2101 cccacaattc ggccgcctcg gtttccgccg taccggtcat gccggccagc ttgtggtaca
     2161 tgcggaaata gttttgcagg gtgactgtcg cataggtctg cgtggccgcc tcgatcttga
     2221 cgttttcctt ggcttcgatc gcctggtgca agccatcgct gtaccggcgc ccatccagga
     2281 tacgtcccgt ctgctcgtcg acgatcttca cctgaccgtc caacacgacg tactccacgt
     2341 ccttgtcaaa cagggtgtag gccttcagca actgctggac cgtgtggatc cggtccgcct
     2401 tgaccgcgta ttcgttaagc agggcttcct tttgctgcag tttttcttca ttgctggcgg
     2461 gcgattgctc gatctccgcg agcttgatgc tgatatcggg aaggatgaag aactccggat
     2521 cttccccttc gcgggtgatc atctggatgc ccttatccgt caggtcgacc gaattgttct
     2581 tttcttctat cgtgaaatac agttccgcgt cgaccttcgg catctccttc gactggtctg
     2641 cgagataata gttttcggtc ttctggagtt tgaccttgat gccgggctcg ctcaggaact
     2701 tgatcagcgg accggacttc ggcaaccccc tgtaggcccg catcagcgcc aggccgccgt
     2761 ccttcgggtc gtcgttccct tcggcaaatt tcttcttggc ctcgatcagg cactggttcg
     2821 cgacccgctt ctggatgtcg aataattttt ccacccg
//
LOCUS       DATGKE010000121         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_800084, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000121 DATGKE010000000
VERSION     DATGKE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800084"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VKU83_02130"
     CDS             complement(<1..1741)
                     /locus_tag="VKU83_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92373.1"
                     /translation="MAGPGDDVTTLTGTVTDKADGKPIAGATISIPDLKTGTVTGADG
                     KFSITVTSRGTHMVEVSYVGYATFNQVVDFSKTNHIDVQLSLATIEAGEVIVTGVSKA
                     TEIRRAPIPIVAVGKTELEQHSAAGNIIDAVANLPGVSAVTTGPNISKPFIHGLGYNR
                     VVTAEDGIRQEGQQWGDEHGIEVDQNSIDRVEIIKGPASLSYGSDAIGGVVNLLTPPP
                     VPDGKILGYASGLYGTNNGLINGSLRLQGNHNGLVWGTQLSQKLAQDYRDRHDGRVYA
                     TNFNEKDARVMIGLNKSWGYSYLNASVFDDEQAIPDGSRDSLTRKFTYQVTDSDTYRP
                     IVPASVLTSYSLPTLHQHVQLYRVYDASSIALGNGNLLVNLGYQYSHRREFTHPRDAT
                     VPGLNLELSTYTYDVKYNFTIGHGYETTFGVNGMYQNNALGSSTDFPIPAYHQFDIGP
                     FITERKSYGRLDISGGVRIDSRNFSGRAAYIDTTTPYFPVLYKGPDPAGTPGVIQQFP
                     ALSKTFSGFSGSLGATYNFTNRFLLKANIARGFRAPSIAELSANGPDPGSQIYHVGNR
                     DFRPEFSVQEDVGL"
     gene            complement(1896..2234)
                     /locus_tag="VKU83_02135"
     CDS             complement(1896..2234)
                     /locus_tag="VKU83_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92374.1"
                     /translation="MFRQYPKLVATLLLLVFTQKAGLRLWMHDWFHEGKVVRAVSCPA
                     AAQIHLQCDCFDDAMMPLLGSPVFTIAPPVCVYAPRTVARQSPVISAGKVYFSLKGPP
                     PPSLFSCRLS"
     gene            2428..>2967
                     /locus_tag="VKU83_02140"
     CDS             2428..>2967
                     /locus_tag="VKU83_02140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLI92375.1"
                     /translation="MKNCLFGLLLLCSVPAMAQPTVRDQFAKIGTVQEAQAWMAANPQ
                     LKPALLHLVAGKDSSLIDKRLLRQNQGDIFAVGYVTYKVIESKESVKYRASYIYLDGS
                     TLSPSELDSLRKVIVDKIRAGANWDQLSDQYTMDGNTTHGDTGWFFGELQMPKEFQDA
                     VKAHNKGDVFFVDVPDRNWH"
BASE COUNT          678 a          763 c          885 g          641 t
ORIGIN      
        1 cgaggccgac atcctcctgc acgctgaatt ccggccgaaa gtcacggttg cccacgtggt
       61 agatctgcga gccggggtcc ggtccgtttg cggaaagttc ggcgatgctg ggcgcccgga
      121 atccccgggc gatgttggcc ttgaggagga agcggttcgt gaaattatat gtggcgccaa
      181 gacttccgga aaatccagaa aaagtcttgc tgagcgccgg gaactgctga atgacgccgg
      241 gcgtccccgc cgggtcaggg cccttgtaca gaacagggaa atagggcgtg gtcgtatcga
      301 tgtacgctgc ccggccggag aagttcctgc tgtcgatgcg tacgccgccg gagatgtcga
      361 gcctgccata gctcttccgt tccgtaatga aagggccgat atcgaactgg tggtaggcgg
      421 ggatcggaaa atcggtgcta gagccgaggg cgttattctg gtacatgcca ttcacgccaa
      481 aagttgtctc gtacccgtga ccgatggtga aattgtattt gacgtcatag gtataggtac
      541 tcagctcgag attgagtccg ggaacggtcg cgtcccgggg atgcgtgaac tcgcggcgat
      601 ggctgtactg gtatcctagg ttgacaagca ggttgccatt gccaagcgcg atgctgctgg
      661 cgtcataaac gcggtacagt tgaacatgct gatgcagggt gggaagggaa taggatgtca
      721 ataccgatgc cggaacgatg ggccggtaag tatccgaatc ggtaacctgg taagtgaact
      781 ttcgcgtcaa cgaatcgcgg ctgccgtcgg gaatcgcctg ttcgtcgtcg aagacggaag
      841 cattcagata ggaatagccc cacgacttgt taaggccgat catgaccctc gcatccttct
      901 cgttgaaatt ggtggcgtat acccggccat catgccggtc ccggtagtcc tgcgccagct
      961 tttgcgaaag ttgggtgccc cagacgaggc cgttatggtt gccctggagc cggagcgagc
     1021 cgttgatgag gccattattc gttccataca ggccgcttgc atagccaagg atcttgccat
     1081 ccggaacggg gggcggcgtc agaaggttaa ccacgccgcc aatggcgtcg gaaccgtagc
     1141 ttaggctggc ggggccttta atgatctcga cacggtcgat ggagttctga tccacttcga
     1201 tcccgtgttc gtcgccccac tgctgaccct cctgccggat accgtcttcc gcggttacga
     1261 cgcgattgta accgaggcca tggataaacg gcttggaaat gttgggaccg gtggtcacag
     1321 cgctgacgcc aggaaggttg gcgaccgcat caatgatatt gccggccgcc gaatgctgtt
     1381 ccaattccgt ctttccaact gcaacgatcg ggatgggcgc ccgcctgatc tctgtcgcct
     1441 tgctgacgcc ggtaacgatc acctctcccg cctcgatggt cgccagcgac agttggacgt
     1501 cgatatggtt ggtcttcgag aaatccacaa cctgattaaa ggtggcatag ccgacgtagc
     1561 tgacctcgac catatgcgtt ccccgggaag taacggtgat ggaaaatttg ccgtctgcgc
     1621 cggttacggt gcctgtcttg aggtccggaa tggagatcgt cgcgcctgcg atcggcttgc
     1681 cgtcggcctt atcggtgacg gtcccggtca aggtcgtaac gtcgtcgcca ggccccgcca
     1741 tttcgcgggt tttggccaaa tcgcgcccgt ttgccaggag cgactgacag ccaaataaga
     1801 atatgagaat gagtgagaat gagtaagact tccggtccat aatccttttt tgattcagca
     1861 gcaatatgtc attaatccag tcggtctgaa agaagtcagg agagtcggca ggagaacagg
     1921 gagggaggag gagggccctt tagggaaaaa tagactttac cggcgctgat aacaggggac
     1981 tggcgcgcga ccgtacgggg tgcatacacg cagaccggcg gcgctatcgt gaagacgggg
     2041 gagccaagca agggcatcat ggcgtcgtca aaacaatcgc actggaggtg gatctgtgcg
     2101 gcggcagggc aggaaactgc gcgaaccacc ttgccttcat gaaaccagtc atgcatccac
     2161 aaacgcaggc ccgccttttg cgtgaaaacc agcaaaagca aagtggccac cagcttggga
     2221 tactgcctga acatgcggtg caatattagt tattatgcaa caatatttca aaaagcgctg
     2281 ctcccgttac cgatcatcaa aaagcctgcc ctttataccg atatggatat aaccacatta
     2341 agaggatgtt aaattgggcc tcgtggctac ctccatatca aaaaatagta acttcagtct
     2401 ttggatcatt cgttaaaata gtaatatatg aagaattgtc ttttcggcct gctcctgctg
     2461 tgttccgtac ccgcaatggc acagccgacg gtccgtgacc agttcgcaaa gattggaacg
     2521 gttcaggagg cgcaggcatg gatggctgcg aatccccaac ttaaaccggc tcttttgcac
     2581 ctggtagcgg ggaaggattc gtcgttgatc gacaaacggt tattgcggca gaaccagggc
     2641 gatatttttg cggtcggata cgttacttac aaggtgatcg aatcgaaaga gtcggttaaa
     2701 taccgcgcca gctatatcta tctcgacggc tccacgttgt cgccctccga actggacagc
     2761 ctgcggaagg tgatcgtgga caagatcagg gcgggcgcga actgggacca attatcggac
     2821 cagtatacga tggacgggaa caccacgcac ggggatacag gctggttctt tggcgaactc
     2881 caaatgccga aggaattcca ggatgcggta aaggcccaca acaaaggcga tgtctttttc
     2941 gtggatgtac ccgaccgcaa ttggcac
//
LOCUS       DATGKE010000122         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_923910, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000122 DATGKE010000000
VERSION     DATGKE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923910"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VKU83_02145"
     CDS             complement(<1..530)
                     /locus_tag="VKU83_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLI92376.1"
                     /translation="MQPGSAPYDVIIAGGGLAGLALSIQLAKAGYSVALFEKENYPFH
                     KVCGEYISLESWNFLQELGLPLSDWNLPVIRHVMVSAPNGLFVRQELPLGGFGLSRYK
                     LDAALAEIARGCGVALRESVKVTEMRVEGSVVAVGTSAETHFSKLACAGFGKRSNLDI
                     KWRRPFTQKRPDRINN"
     gene            complement(517..1239)
                     /locus_tag="VKU83_02150"
     CDS             complement(517..1239)
                     /locus_tag="VKU83_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLI92377.1"
                     /translation="MASFSNRSHDKELLDQERIPFADIEQNMRELDLINTWLGGHRIS
                     LLGLKRLLGGRRRITICEIGCGGGDNLRVLDRYCRRRGIDAELIGVDSNESCIAVARQ
                     RWRGGHAEWIHSDFRDVIFEGKKPDIIFSSLFCHHFTDRELVYMLRWMEMNCAAGWYI
                     NDLHRHPVAYHAIRWLTGWLSRSYLVKNDAPLSVLRGFTRAEWMAILREAGIQDYRLE
                     WKWAFRWLLYRPAGLSILYAAG"
     gene            complement(1241..2323)
                     /locus_tag="VKU83_02155"
     CDS             complement(1241..2323)
                     /locus_tag="VKU83_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HLI92378.1"
                     /translation="MSKIISIGTAVPAYRHRQEDILPFMQRIYAGDSQASRKLKHLYR
                     QSGIESRYSVIPDYTLPAADWEFYPPTENLEPFPSLEQRMSIYRRHAAPLSVAAVRDC
                     LQGKLEVRAITHLITVSCTGMSAPGLDLELADALGLPVTLFRTSVNFMGCYAAVHALK
                     LADALAGSDPGARVLVVCTELCTLHFQQEPTVDNMLSSLLFADGSAAVLVTADNYPGG
                     GLKIKGFYSEIVPGGKDEMGWHLSSTGFRMTLSNYVTDLIEADFGGLVGRVLQRGGLE
                     QDSIGHWCIHPGGKKILDAIAKSLSLRNGQLDDSYAILGAYGNMSSPTILFVLRRMMS
                     RPDPGRILGAAFGPGLTMETFIAETN"
BASE COUNT          525 a          725 c          656 g          487 t
ORIGIN      
        1 taattgttga tccggtccgg tcttttttgt gtaaagggcc tgcgccattt gatgtccagg
       61 ttactgcgct ttccaaaacc cgcacaagcc agcttcgaaa aatgcgtctc cgcgcttgtc
      121 ccgacggcaa ccaccgaccc ttcgacccgc atctccgtca cctttaccga ttcgcgcaag
      181 gcgacgccgc agccgcgggc gatttcggcc agggctgcat ccagcttata ccggctgagg
      241 ccgaagcctc ccaacggcag ttcctgcctt acgaagagcc cattaggcgc ggaaaccatt
      301 acgtgacgga tgaccggcag gttccagtca ctcagtggca gacccagctc ttgaaggaaa
      361 ttccagcttt ccaggctgat gtattcgccg caaactttat ggaagggata attctctttc
      421 tcgaagaggg cgacggaata gcccgccttc gccagctgga tcgaaagggc gaggcccgcc
      481 agaccgcccc ctgcgataat gacgtcgtat ggcgcgctac ccggctgcat acaatatgga
      541 taggcctgcc ggccgataca gcaaccagcg gaaagcccat ttccactcca gccggtaatc
      601 ctggatccct gcttcccgca ggatcgccat ccattccgcg cgggtgaagc cccgcaggac
      661 ggacaacggc gcgtcatttt tgaccaggta ggatcgcgac agccatccgg tcaaccagcg
      721 gatagcgtga taggcgacgg gatgccggtg gaggtcattg atataccagc ctgccgcgca
      781 attcatttcc atccagcgga gcatgtagac gagttcccga tccgtaaaat gatgacaaaa
      841 cagcgaggag aaaatgatat ccggcttttt cccttcgaag ataacgtcgc ggaaatcgga
      901 atgtatccac tccgcatgtc caccgcgcca tcgctgcctc gcgaccgcga tgcatgattc
      961 gttgctgtcg acgccgataa gttccgcgtc aattccccgc cggcgacaat aacggtccag
     1021 gacgcgcagg ttgtctccac cgccgcagcc aatctcgcag atcgttatcc ggcgccggcc
     1081 gcccaacagc cgtttcagcc cgagcagact tatccggtgt ccgcccagcc aggtgttgat
     1141 gaggtcgagc tcacgcatgt tctgttcgat gtcggcaaag ggaatccttt cctgatcgag
     1201 caattccttg tcgtggctcc tgttggaaaa ggaggccatg ctagttggtt tccgcaataa
     1261 aagtttccat cgttagaccg gggccaaagg cagcgcccaa tatccggccg gggtccggcc
     1321 tgctcatcat ccgccggagg acgaaaagta tggtggggga tgacatgttc ccataagccc
     1381 cgagaatggc ataactgtcg tcgagctgtc cattccgcag tgaaaggctt ttggcgattg
     1441 catccagaat tttctttccg cccggatgga tgcaccaatg accgatgctg tcctgctcca
     1501 ggccgccgcg ctgcaatacg cgccctacga ggccgccgaa gtcggcttct atcaggtcgg
     1561 ttacgtagtt gctgagggtc atccggaatc cggtagagga aagatgccag cccatctcgt
     1621 ccttaccacc cggcacgatc tccgaataaa atcctttaat tttcagcccg cccccaggat
     1681 aattgtccgc tgtgacaagg accgctgcgg aaccgtccgc aaataaaagg ctggacaaca
     1741 tattatcgac cgtgggctcc tgctggaagt gcaaggtgca gagttccgta cagacgacca
     1801 gcacgcgggc gccggggtcg gagcccgcca gcgcgtcggc cagcttcagc gcatgtacgg
     1861 ccgcgtaaca tcccataaaa ttgaccgacg tgcggaacaa cgttacggga aggcccaatg
     1921 catccgccaa ctccaggtcc aatcccgggg cgctcatgcc ggtacaactg acggtgatca
     1981 gatgcgtgat ggcccgcacc tccagtttgc cctgcaggca atctcgcacc gccgccaccg
     2041 acaaaggcgc ggcgtggcgc cggtagatcg acatccgctg ttcgagggaa gggaatggct
     2101 ccaggttttc tgtcggcggg taaaactccc agtcagccgc cggcagggtg taatcgggga
     2161 tgacagaata gcgcgattca atcccactct gacgatacag gtgcttcagc ttcctgcttg
     2221 cctgactgtc tcccgcatag atccgttgca tgaaaggaag aatatcttcc tggcgatggc
     2281 ggtaggccgg aaccgcagtt ccaatggaaa tgatcttgct caaaatatgc gcttatttga
     2341 ctacttattg atcgctgcaa agatgggacc agctatcgat caccagccgc cga
//
LOCUS       DATGKE010000123         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_175774, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000123 DATGKE010000000
VERSION     DATGKE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175774"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VKU83_02160"
     CDS             <1..835
                     /locus_tag="VKU83_02160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLI92379.1"
                     /translation="GIDIRQVAGSGDNGRIVKKDVDNFTPGTVAGGGAQPASGAQPAA
                     ASAPAGQVSFEEVPVSQMRKAIAKRLAESKFSAPHFYVTMTVDMDKAIESRLKLNEVS
                     PVKISFNDLVLKAVAVALKQHPAINSSWLGDKIRINHHVNIGVAVAIDDGLLVPVVRF
                     ADTKTLSQIAAEVKDLAQRAKSKKLQPADWEGSTFTISNMGMYGVDEFTAIINPPDAC
                     ILAVSSIQAVPVVRNGAVVPGNIMKLTLSCDHRVVDGAKGSEFLQTVKSLLEEPLRMM
                     L"
     gene            1290..>2743
                     /locus_tag="VKU83_02165"
     CDS             1290..>2743
                     /locus_tag="VKU83_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLI92380.1"
                     /translation="MSFKQLNLIEPLLKALEKEGYTVPTPIQEQAIPIILKRRDLLGC
                     AQTGTGKTAAFALPLLQLMCEDATHADGHPGTAGPIRALVLTPTRELAIQIDESFGAY
                     GKFTRVRHTVIFGGVPQNPQVQALQQGVDVLVATPGRLLDLMNQGYINLQQIRYFVLD
                     EADRMLDMGFIHDIRKVLAKLPRKKHSLFFSATMPPEIQKLAGTILVNPEQVEVTPVS
                     STAETVRQSIYFVEKKDKRSLLLHVINEGQIPSALVFTRTKHGADKVAQGLLRAGIRA
                     EAIHGNKSQNARQRALANFKSRQTRILVATDIAARGIDIDELTHVINFELPNVPETYV
                     HRIGRTGRAGASGTALSFCDQEETEYLRDIQKLIARAIPVVEDHPHVMAAGLRSRAPV
                     VATTMPADRNDGRQDLRHAGMRREETKRRDEARRHEERKRREQVKPHAEPKRGEWKGG
                     EEPKRREPVKHPEEPKRREPVKHPEKQKPREHVER"
BASE COUNT          641 a          748 c          799 g          555 t
ORIGIN      
        1 agggattgat atcaggcagg tagccggtag cggcgacaac ggccggatcg tcaaaaagga
       61 cgtcgacaac tttactcccg gtacggtcgc cggcggcggg gcgcaacctg cttcgggcgc
      121 ccaaccggca gcggcttcag cgccggccgg tcaggtcagt ttcgaggagg tgccggtatc
      181 gcagatgcga aaagcgatcg caaagcgact ggcggaaagc aaattttcgg cgccgcattt
      241 ttatgtgacg atgacggtgg atatggataa ggccatcgaa agccggctga agctgaatga
      301 ggtttcgccg gtaaagatct cttttaacga tctcgtgctg aaggcggtgg cggtggcatt
      361 gaaacagcat cctgcgatca acagcagttg gcttggcgat aagatccgga tcaaccacca
      421 tgtcaatatc ggcgtagccg tggccatcga cgacggcttg ctggtacctg tcgtgcgttt
      481 tgcggacaca aaaacgcttt cccagatcgc ggccgaggtc aaggacctgg cgcagcgggc
      541 gaaaagcaag aagttgcagc cggctgactg ggaaggaagc acgttcacta tttccaatat
      601 gggcatgtat ggcgtggatg agttcacggc aatcatcaat ccgccggatg cctgcattct
      661 ggccgtgagc tcgatccagg ccgtcccggt cgtcaggaac ggcgccgtgg tgccgggcaa
      721 tatcatgaag ctgacgctga gctgcgacca ccgcgtcgtg gatggggcca agggctccga
      781 gttccttcag acggtcaaga gcctgctcga ggagccattg cgaatgatgc tgtaggctgg
      841 ctgcagggcc gcctgaaagt ataaagccgc cccatagaca tgaggcggct ctttaaacct
      901 tatcctattg ggtttcctgt agaaacaggc agcccttaga actgtgtcct tgctgttcca
      961 accaaactta aattcttgat ttttcgattc acctgcggcg ggttgcctga tccgccgcta
     1021 acgatcacaa atataggaac tttctcgatt ctgcggtctt tttagaccac aatttcgaaa
     1081 attttttcgc ccttttttat agcgcaggga caatggttat caggggcatc gtccggcggc
     1141 caggattttg tcgcggttat tcggcgcaaa atacctaact ttgcacccgt tatactcctt
     1201 tctattcact ttcgtgacaa ccttctgtga taacgcccga aggataatgc tgtgtagatg
     1261 ggctttttac aacttaaata gattttattt tgtcgttcaa acaactcaac ctcattgagc
     1321 ctttgctaaa ggctttggaa aaagagggat ataccgtccc tacgcctatc caggaacagg
     1381 ctataccgat aatccttaag cgcagggatc tgcttggttg tgcccagacg ggcaccggca
     1441 agaccgcagc ctttgcgctc cccttgttac aacttatgtg cgaagacgcg actcacgccg
     1501 acggacatcc ggggactgcc ggccctatcc gggcgctggt gcttacccca acccgcgagc
     1561 tggcaatcca gatcgacgaa agctttgggg catacggaaa gttcacccgg gtcaggcata
     1621 cggtcatctt tggcggggta ccgcaaaacc cgcaggtaca ggctttgcag caaggcgtag
     1681 acgtattggt tgctacgccc ggaaggttac tcgatctcat gaaccaggga tacatcaacc
     1741 tgcagcagat ccggtacttc gtgctggatg aggccgaccg catgctggac atgggtttca
     1801 tccacgatat tcgcaaagta ctcgccaaac tgccccgcaa aaaacattcg ctcttctttt
     1861 cggctaccat gccgcccgaa atccagaagc ttgcgggcac gattctcgta aaccccgaac
     1921 aagtcgaggt cacgcctgtt tcttcgaccg cggagacagt ccggcaatcc atctattttg
     1981 tagagaagaa agacaagcgt tcgctcctgc tgcatgtcat caacgaaggc caaatccctt
     2041 cagcgctggt ttttacccgt accaagcatg gcgctgacaa ggttgcgcag ggcctgttgc
     2101 gggctgggat ccgcgcggaa gcgatccacg gcaacaaatc tcaaaatgcc cggcagcgcg
     2161 cactggcgaa cttcaaaagc cgccaaaccc gcattttggt ggcgaccgat atcgccgccc
     2221 gcgggatcga catcgatgag ttgacgcatg tgattaattt cgagctgccg aacgtaccgg
     2281 aaacttatgt gcaccgcatc ggaaggaccg ggcgggccgg ggcgagcggg acggcgctct
     2341 ctttttgcga tcaggaagaa acagaatatc tccgtgatat ccagaagctg attgcgcggg
     2401 ctatcccggt agtggaagac catccgcatg tcatggccgc cgggctgcgt agccgggcgc
     2461 cagtcgtcgc gacaaccatg ccggccgacc ggaacgacgg gcggcaggat ctgcggcatg
     2521 ccgggatgcg ccgggaagaa acaaaacgcc gcgacgaggc aaggcggcat gaggagcgga
     2581 agcgaaggga gcaggtcaag ccgcacgcgg aaccgaagcg cggggaatgg aaaggggggg
     2641 aagaacccaa gcgccgtgag ccggtcaaac atcccgaaga gccgaagcgc cgtgagccgg
     2701 tcaaacatcc ggaaaagcag aagccgcgcg agcacgtcga acg
//
LOCUS       DATGKE010000124         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_692144, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000124 DATGKE010000000
VERSION     DATGKE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692144"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..561)
                     /locus_tag="VKU83_02170"
     CDS             complement(40..561)
                     /locus_tag="VKU83_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HLI92381.1"
                     /translation="MSVLEKFKSIRTFAFDVDGVMTDGSVLLYQSGEQVRRMSTRDGY
                     SLQLAVHKGYRVVVVSGALSEGVRQRLLYLGIKDIFLETHDKAGILRQYAADHGLDVN
                     DMLYMGDDIPDFHPMQLVGLACAPADAAPEIRHIAAYISSFNGGQGCVRDVIEKVLKL
                     NGHWDLNATVASK"
     gene            complement(539..1321)
                     /locus_tag="VKU83_02175"
     CDS             complement(539..1321)
                     /locus_tag="VKU83_02175"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HLI92382.1"
                     /translation="MKTVIIGSGNVASVFGEKIVAAGHEVCQLVGRSAGPAAAVARSL
                     GCPWTTAWPEVDRTAGLYVVAISDSALTTLAKSITLPGKLVVHTAGSAAASALAAVSE
                     RCGVIWPLQSTRAEVRPYPPMPLIVDAHRPEDLPEIAGFAGSLSEKVVTAADDARLNL
                     HVAATLVNNFTNYLYSLAAGFCDRQRIDFGLLLPLIRETAGRLDRYEPEALQTGPARR
                     GDVPTIARHMNILRDFNDIKQLYELFTIQIEEHYRYERTGKI"
     gene            1320..2228
                     /locus_tag="VKU83_02180"
     CDS             1320..2228
                     /locus_tag="VKU83_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLI92383.1"
                     /translation="MLYFCMGGMPGKSMKLKQKLSTRYLRAQLNILSAVSPRKSAVKA
                     FRAIIRPRRRQKGKPPKIFETGEPLSFRFDGHTVRGHRWQPYSDDLRPTKEAAKKVLM
                     VHGFESSTKVFGHCITALLKKGFEVVAFDAPGHGASGGKRLFVSDYARMLTQIEEMFG
                     PFQAWLAHSLGGLALALSLEDTQPEGRSALVLVAPAPGVARAAEEWTARLRLPADVLR
                     EMDEYVMETSGHPLGWYSLRRVIGSVGAEILYVQDEEDPVTPPEEARLIEQDRHPNIQ
                     FVFTTGLGHKKIFKDQEVLDRIVEFL"
     gene            complement(2225..2881)
                     /locus_tag="VKU83_02185"
     CDS             complement(2225..2881)
                     /locus_tag="VKU83_02185"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLI92384.1"
                     /translation="MRTYEDTYRHKGLRRQLVEVIRGKGITDERVLHAILHIPRHFFM
                     DSAFDKIAYEDRAFPIGEDQTISQPYTVAYQTQLLEVKPFDKVLEIGTGSAYQACVLA
                     EMGATVYTIERQKKLFDANRQFGYLKKYPALKFFYGDGYEGLPTFAPFDKIIVTAAAP
                     FIPPRLIEQLKTGGRMVIPVGEGNVQRMLRLTLQSDGNIVEETFDNFSFVPLLGGKNS
                     "
     gene            3016..>3446
                     /locus_tag="VKU83_02190"
     CDS             3016..>3446
                     /locus_tag="VKU83_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI92385.1"
                     /translation="MKTTTRIQLSVLMFLEFFIWGAWYTMIGVYMTNHGMPKLTQWPF
                     TVNPIAAIIAPFFIGLIADRYFATQKVMGVLHIIGSLFMFLVPSFTDKPLIFILLLLG
                     YNICYMPTMGLANTVCFYKITDQKKEFPLLRVFGTIGWIVAG"
BASE COUNT          748 a          959 c          978 g          761 t
ORIGIN      
        1 ggacaaggcg aaagaaagcg gaaaataaag ccataccatt tattttgagg cgaccgttgc
       61 attcaggtcc caatgaccat tcagcttgag cactttttcg atcacgtccc ggacgcagcc
      121 ctggccgccg ttaaacgacg aaatgtaggc ggcaatatgc cggatctcag gcgcagcgtc
      181 tgccggggcg caggccagcc cgaccaactg catgggatgg aagtcgggga tatcgtcgcc
      241 catatataac atgtcattga catcgagtcc atgatcggcg gcatactgcc gcaggatacc
      301 cgccttgtcg tgcgtttcga ggaagatatc ctttattccc aaatagagta gccgctgccg
      361 gaccccttcc gacaacgcgc ccgaaaccac gactacccga tatcctttgt ggacggccaa
      421 ctgcagcgaa tagccgtccc gggtgctcat tctacggacc tgttcgccag attgatatag
      481 cagtacgctg ccatcggtca ttacgccatc gacgtcaaac gcaaatgtcc ggatcgactt
      541 aaatttttcc agtacgctca taacggtaat gctcttctat ttgaatagta aataactcgt
      601 ataactgctt gatatcattg aaatcgcgca aaatattcat atgcctggcg atggtcggaa
      661 cgtcgccccg ccgcgccggc cccgtttgca atgcttccgg ctcgtaccgg tccagccggc
      721 cggcggtctc gcggatcagg ggcaacagca agccgaaatc gatccgttgc cgatcgcaaa
      781 accccgcagc gaggctgtaa agatagttcg taaaattgtt taccagggtg gcggcgacgt
      841 ggaggttcag ccgggcatca tcggccgccg tcaccacctt ttcggaaagg ctgcccgcga
      901 agccggcgat ctccggcagg tcttcagggc ggtgggcatc gacaataagc ggcatgggcg
      961 ggtacggccg aacttcggcc cgcgtgcttt gaagcggcca gatgacgccg caccgttcgc
     1021 tgacagcggc gagtgccgac gcggccgccg agccggcggt gtggacgacg agctttccgg
     1081 gcagggtaat gctcttagca agggtcgtca gagcgctgtc gctgatcgcg accacataga
     1141 ggccggctgt ccggtcgacc tcgggccagg ccgtagtcca gggacaaccc agggacctgg
     1201 cgaccgcggc agcgggacct gcactccggc caacgagctg gcaaacctcg tggcctgccg
     1261 ccactatctt ttccccaaat accgatgcga cgttgcccga gccgataata acggtcttca
     1321 tgctttattt ttgtatgggc ggtatgcccg ggaagagcat gaaattaaag caaaaacttt
     1381 caacgcggta cttgcgggcg cagctaaata tactttcggc tgtcagcccg cgtaagtcgg
     1441 ccgtgaaggc gttccgagcc atcatccggc cgcgacggcg ccaaaaaggc aaaccgccga
     1501 agatttttga aacgggcgag cccctatcct tccggtttga cggccatacg gtccgcgggc
     1561 accgttggca gccgtattcg gatgacttgc ggccgacgaa ggaggccgca aaaaaagtcc
     1621 tcatggtgca cgggttcgag tcgtcaacga aggtgtttgg tcactgcatc accgccttac
     1681 taaaaaaggg gtttgaagtg gtggccttcg atgcgccggg acatggggcg tcgggtggaa
     1741 agcgcctttt tgtgtcggat tatgcgagga tgctcactca aatagaagaa atgttcggtc
     1801 cgttccaggc atggctggcc cattccctcg gcggtctggc gttggcgctg tcgctcgagg
     1861 acacccagcc ggaaggccgg tctgcgctgg tgctggtcgc gccggcaccg ggagtcgcca
     1921 gggcggcgga agaatggacg gcccggctca ggctgcctgc agacgtcttg cgggagatgg
     1981 atgaatacgt catggagacc agcggtcatc ccctgggctg gtattcgctc cgccgcgtca
     2041 tcggttccgt aggggccgag atcctgtatg tgcaggacga ggaggatccg gtcacacctc
     2101 cggaagaggc acgattgatc gaacaggacc ggcacccgaa catccagttt gtttttacaa
     2161 ccggccttgg ccataaaaaa atatttaaag accaggaagt ccttgaccgg atcgtcgaat
     2221 ttctctagct gttcttcccg ccgagcagcg gaacaaaaga aaaattatca aaggtctctt
     2281 cgacgatgtt gccgtcgctt tggagcgtca gtcggagcat ccgctggaca ttcccctcgc
     2341 caacggggat gaccatgcga ccgccggtct tgagctgttc gatcaggcgg ggcggaataa
     2401 aaggcgccgc ggcagtgaca atgatcttgt cgaacggggc aaaagttggc aagccctcat
     2461 agccgtcgcc gtaaaaaaac ttcagcgcgg gatatttctt caggtaaccg aactgccggt
     2521 tggcatcaaa gagtttcttt tgcctttcga tggtatagac agtagcgccc atttcggcca
     2581 gcacgcacgc ctggtaagcg ctgccggtcc cgatctccag cactttgtca aacggtttca
     2641 cctcgaggag ctgggtctga tacgcgacgg tgtacggctg agagatggtc tggtcctccc
     2701 ctatcgggaa cgcgcggtct tcatacgcga tcttgtcgaa tgcggaatcc atgaaaaaat
     2761 ggcggggaat gtgtagaatg gcatgaagga cccgttcgtc ggtaatgccc ttgccgcgga
     2821 tcacctcgac caattgccgc cggaggccct tatgccggta tgtatcttcg tatgttctca
     2881 tcccggctgc aagatactgt gcggggggat gccgggtcac tgacctttca gaaatttttt
     2941 acaggatacg tttttcggtt gtatcttaac cggccggagg cgtttaattt ttcccgcaat
     3001 tcaatcacta gagcaatgaa aacaaccacc agaattcagc tttcagtcct catgttcctg
     3061 gaatttttta tctggggggc ctggtacacc atgataggtg tctatatgac caaccatggg
     3121 atgccgaaac tcacccaatg gcctttcacc gtaaacccca tcgccgccat tatcgcgcct
     3181 ttctttatcg gcctgatcgc cgaccggtat ttcgccaccc aaaaggtgat gggtgttctg
     3241 cacattatcg gttcgctctt tatgttcctt gtgccgtcct ttacggataa gcccctgatc
     3301 tttatcctcc tcttgctggg atacaatatc tgttatatgc cgaccatggg acttgccaat
     3361 acggtgtgtt tctataagat caccgaccaa aaaaaggaat tcccgttgct tcgggttttc
     3421 gggaccatag gctggatcgt ggcagg
//
LOCUS       DATGKE010000125         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1234044, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000125 DATGKE010000000
VERSION     DATGKE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234044"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VKU83_02195"
     CDS             <1..380
                     /locus_tag="VKU83_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI92386.1"
                     /translation="YLALFVTNTPLNVGSYTGLIMIVGIIGENAIFTYLQFRDSLATM
                     SVDDAVIYAISTRLRPKLMTALGAIIALLPLALGIGTGAQLHQPLAISVIGGFIIALP
                     LLLIVLPTLLRLVYRRKKPATIA"
     gene            373..909
                     /locus_tag="VKU83_02200"
     CDS             373..909
                     /locus_tag="VKU83_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI92387.1"
                     /translation="MRSLPPMEIRISMAGKEEATVIADMSIRTFEETFASHNTAENME
                     HFLRTQFTREILIQQVGAPRNDFLLAWLDDRLAGYARLYDSPELPRELSGTRAIEVSR
                     FYAEQFAIGKGVGKSLMEACFDLARKREKEWIWLGVWEKNHRAIAFYEKMGFRIFGRH
                     IFLLGQDVQHDWEMKRKL"
     gene            complement(904..1506)
                     /locus_tag="VKU83_02205"
     CDS             complement(904..1506)
                     /locus_tag="VKU83_02205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLI92388.1"
                     /translation="MFISEDQLHLLRGKSVYLEFLKQEHREQLRSLARDERLWEFTKT
                     LLITDSFDHQFNKYFDDALAFAARGDQAFAILETGTRRPIGMTRAYDVDFRVKKVTIG
                     HTWYVPEVWGRAHNKECKLLLLRYIFDTLGFERTDFKVAGENLRSQRAVEKIGGVKEG
                     VLRRFAMRNDGTPADTVYFSILGNEWPERRQRLQAMVDAL"
     gene            complement(1572..2285)
                     /locus_tag="VKU83_02210"
     CDS             complement(1572..2285)
                     /locus_tag="VKU83_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI92389.1"
                     /translation="MTGKIKCVIIDDEPFARKGLQGYAGQIDFLDLRGMCENALELNS
                     MLKREPVDLLFLDIEMPYLTGIEFLRQIAVKPKVIFTTAYEKYALQGFELEVLDYLLK
                     PIPFERFLRAANKAWDYFNGQQSAAVVQDFIFVKADGKLEKILLADILFAEALENYVA
                     IYTREKKTITHLTLKMLQEKLPPAAFVQPHKSYLIAIDKIKSIEGNILHIDRYQVPVS
                     KYQKDEILEKIVNSKLLKR"
     gene            complement(2288..>2527)
                     /locus_tag="VKU83_02215"
     CDS             complement(2288..>2527)
                     /locus_tag="VKU83_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92390.1"
                     /translation="AFKYVGGEYQLRIAAGIETGAIRFEVANSVPADLPANRQGGIGL
                     DNLKRRLELLYPGRHCLTAGRKGDRFEAVLTIQYQ"
BASE COUNT          530 a          734 c          664 g          599 t
ORIGIN      
        1 gttacctcgc cctgttcgtg accaatactc cgctgaatgt aggcagctat acgggactca
       61 tcatgattgt cgggatcatc ggcgaaaacg cgatttttac ctatttgcag ttccgggatt
      121 cgttagctac catgtcggtg gacgatgcgg tgatctacgc gatttccacc cggttacggc
      181 caaagctcat gacggcgctg ggggccatca tcgccctcct tccgcttgcg ctgggcatcg
      241 gcacgggcgc tcaattgcac cagccgctcg cgatctcggt tatcggcggg ttcatcattg
      301 ccctgcccct gttgctgatc gtcctgccca cgttgctgag gctcgtttac cgccgtaaga
      361 aaccggccac aattgcgtag cttgcctccc atggagatcc ggatcagcat ggctggcaag
      421 gaggaagcaa cagtcatcgc cgacatgagc atccgcacgt tcgaggagac cttcgcctcg
      481 cacaacacgg cggagaatat ggaacatttc ctgcgcactc agttcacccg ggaaatcctg
      541 atccaacagg tcggcgcccc ccgcaacgac tttctcctcg cctggctcga cgaccgcctt
      601 gccggctatg cgcggctcta cgatagtccc gaacttcccc gggaattatc cgggacgcgg
      661 gcgatcgaag tttcgcgctt ctatgccgaa cagtttgcga tcggaaaagg tgtcggaaaa
      721 tccctgatgg aagcttgctt cgacctggcc aggaagcggg agaaggaatg gatctggctt
      781 ggcgtttggg aaaagaacca ccgggcaatc gccttttacg agaagatggg ctttcggatc
      841 ttcggccgcc atatttttct gcttgggcag gacgtccaac atgactggga aatgaaaagg
      901 aaattatagc gcgtcgacca tcgcctgcaa ccgttgtctt ctttcgggcc actcattgcc
      961 aaggatactg aaataaacgg tatctgccgg ggtgccgtca ttccgcatgg caaacctccg
     1021 caaaacaccc tctttcacgc ccccgatctt ttcgaccgcc cgttgcgacc gcagattctc
     1081 ccccgccact ttgaaatcgg ttcgctcgaa acccagcgtg tcgaaaatat accgcagcag
     1141 caggagctta cattccttgt tatgggccct cccccagact tcggggacgt accaggtgtg
     1201 gccaatggtg acctttttca cgcggaagtc gacatcatat gcccgcgtca tgccgatggg
     1261 ccggcgggtt cccgtttcca ggatcgcaaa cgcctggtcg cccctggccg caaacgccag
     1321 cgcgtcatcg aaatacttgt tgaattggtg gtcgaaggaa tcggtgatga gcaaggtctt
     1381 cgtaaattcc cacagacgct cgtctcttgc aagcgacctg agctgctcgc ggtgctcctg
     1441 ctttaaaaac tcaaggtaaa cgcttttccc cctcagcaga tgcagttgat cttcgctgat
     1501 gaacatgcag caaatatagc cctattccac caatattttg cccgaatccg gcagcccgtg
     1561 ctgtggcgca cctaccgttt taacaatttc gaatttacga tcttttccag gatctcgtcc
     1621 ttctgatatt tgctgaccgg cacctggtac cggtcaatgt gcaggatatt tccctcgatg
     1681 gatttgatct tgtcgatcgc aataaggtac gacttatgcg gctggacaaa ggccgccggg
     1741 ggcagtttct cctgtagcat ttttaacgtc agatgcgtga tggtcttctt ttcccgggta
     1801 tagatcgcga cataattctc gagcgcctcg gcaaacagga tgtcggcgag gaggattttt
     1861 tccagcttgc cgtccgcctt gacgaaaata aagtcctgca cgacggctgc ggactgctgg
     1921 ccgttgaaat aatcccaggc cttattggcc gcgcgaagga agcgctcgaa cgggatcggt
     1981 ttcaacaggt agtccagtac ttccagttcg aacccctgca gggcatattt ctcgtaggcg
     2041 gtggtaaaga tcaccttagg cttaacggcg atctgcctga gaaattctat gccggtaagg
     2101 taaggcattt cgatgtccag gaacaggagg tcgacgggct cacgtttcag catcgagttc
     2161 aactccagcg cattttcgca catgccgcgc agatccagaa aatcgatttg accggcataa
     2221 ccctgcaatc ccttgcgggc aaaaggctca tcgtcaatta tgacacattt gatctttccg
     2281 gtcatcgtta ttggtattga atggtgagca ctgcctcaaa ccggtcgccc tttctcccgg
     2341 cggtcaggca gtggcggccg ggatagagca gctccagccg tcgtttcagg ttgtccagcc
     2401 cgatcccgcc ttgcctattc gccggcaggt ccgccggtac gctgttggcg acctcgaagc
     2461 ggatagcccc cgtttcgatg cccgcagcta tccttagctg atactcgccg ccgacatatt
     2521 taaaagc
//
LOCUS       DATGKE010000126         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1518349, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000126 DATGKE010000000
VERSION     DATGKE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518349"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VKU83_02220"
     CDS             complement(<1..329)
                     /locus_tag="VKU83_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92391.1"
                     /translation="MSGHSNGFDPAEVNARPDSPWKPGPKEFIFRYLKYLPWVLVTLV
                     FFMILAYLKVRYATSIYRVQSSLLIKNDRSENRASDARFDELFMSQGVTNLSNEIEIL
                     KSKPVLQR"
     gene            complement(348..1199)
                     /locus_tag="VKU83_02225"
     CDS             complement(348..1199)
                     /locus_tag="VKU83_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HLI92392.1"
                     /translation="METTKQGRKCLPLIYVLALGFSAGLSASCGSTRPFTYMQGQFDT
                     AKFSQIPVADPAIQKSDLLSIIVFSDNPQATALFNQPMIGAASSSGGLGGAAGMASTP
                     GTTGGAGVPLTTAGYLVDQRGDITFQGIGLLHVEGLTRSQLIDTLNDRLKQFLTNPYC
                     TVRFLNNRFTMLGEVAKPGMYTIPGDQLNILEAIGLAGDLTFYGRRDNVLIVRENNGK
                     REFARLDLTKPDIMGSPYFYLQQNDMVIVEQNKKKAVANDQVATRNISIAATIVSTLA
                     ILYSIFR"
     gene            complement(1186..1695)
                     /locus_tag="VKU83_02230"
     CDS             complement(1186..1695)
                     /locus_tag="VKU83_02230"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HLI92393.1"
                     /translation="MRVDKRNWYAIYTKPRWEKKVYGRLVEKGFEAYCPLNRVRKKWS
                     DRLKWVEEPLFKSYVFVKVEEEELAAVRMVNGVVNFVYWLGKPAIIRNKEIEVIHKFL
                     SEYDEVQVVPVDLRTESRITILQGALMDKKATVIKVLNNKVRVVIESIGYSLVATIDK
                     SNVILNGNH"
     gene            2007..>2609
                     /locus_tag="VKU83_02235"
     CDS             2007..>2609
                     /locus_tag="VKU83_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HLI92394.1"
                     /translation="MITGNLSQAGFHASDNLKATALMLMFFLFSFTSAAQYDSTVVGR
                     TMQSLRNVKTDRAEGYYITDPGKQGWFVGDPGDTKTPDDSVMTLVAANGMRFKRTDKG
                     DIDVRWFGARGDGKSDDWFCIQKAITFILEHNTASRTLSFPAGTYSLSRPLIIARFTG
                     NSYKQASINLVGPANSKTISAGYAVLAPTFNNTFAIGVQLG"
BASE COUNT          645 a          704 c          635 g          625 t
ORIGIN      
        1 cgctggagca ccggctttga cttcaggatc tctatctcat tgctgaggtt ggtgaccccc
       61 tgcgacatga ataactcgtc aaaccgggca tcggacgccc ggttctcaga acggtcattt
      121 ttaatgagca gggaagactg aacgcggtaa atactcgtag cgtagcggac cttcagatag
      181 gccaggatca tgaaaaatac caacgtcacc aatacccagg gcaggtattt gagatagcgg
      241 aagataaatt ccttcggacc cggtttccag gggctatcgg gccttgcatt gacctcggct
      301 gggtcgaaac cgttggagtg gccgctcata ttgaagatct gtccatttta cctgaatata
      361 gaataaagga tcgccagggt agaaacaatg gtggccgcga tcgaaatatt ccgggtggcg
      421 acctggtcat tcgccaccgc tttcttcttg ttttgttcca cgatcaccat atcattttgc
      481 tggaggtaga aataaggaga gcccatgatg tccggcttgg tgaggtcgag cctggcgaat
      541 tcgcgcttcc cgttgttctc acggacgata agcacgttat cccgcctgcc gtaaaaagta
      601 agatcgcccg cgaggccgat ggcttccagg atattgagct ggtctccagg aatcgtgtac
      661 atccccggct tggccacctc tcccagcatc gtaaaccggt tgttaagaaa gcggacggtg
      721 cagtacgggt tcgtcaggaa ttgctttagc cggtcgttca aggtatcaat cagctggctg
      781 cgggtcaggc cctctacgtg taagaggccg atgccctgaa aggtgatatc gccgcgctgg
      841 tccaccaggt aaccggcggt agtcagcggc actcccgcgc cgccggttgt gccgggcgtc
      901 gacgccattc cggccgcccc acccagcccg ccggaactgc ttgcagcgcc aatcatgggt
      961 tgattaaaaa gggcggtggc ctggggatta tcactgaaaa cgatgatgct gagcaagtcg
     1021 gatttttgga tggcgggatc cgccaccggt atctggctga atttggccgt gtcaaactgt
     1081 ccctgcatgt aggtgaacgg cctcgtgctg ccgcaggaag ctgataagcc ggccgaaaac
     1141 ccaagggcca atacatagat gagcggaaga catttccgcc cctgtttagt ggtttccatt
     1201 taatattacg tttgacttat cgatggtagc caccagggaa tatccaatgc tctcgatgac
     1261 cacgcggacc ttattgttca gcacttttat aacggtcgct tttttatcca tcagggcgcc
     1321 ctgcaggatc gttatcctgg actccgtccg caggtcgacg ggcaccacct gcacctcgtc
     1381 atactcgctc agaaacttat ggatcacctc gatttcctta ttcctgataa tggcgggctt
     1441 gcccagccaa tacacaaaat tcaccacccc gtttaccatc cgtactgcgg ctagttcttc
     1501 ttcctccact ttcacgaaga cataggactt gaacaagggc tcttcgaccc atttcagccg
     1561 gtcactccat ttcttccgca cccggttcaa aggacaatag gcttcaaatc ctttttcaac
     1621 cagccggcca tacactttct tctcccaccg gggcttggta tatatcgcat accaattcct
     1681 tttgtctacc cgcatagctt cgcctcctca gtcacaggaa aatattgcaa ctgcttgatt
     1741 ttaactaaac taaacccttt gccctccaat gcccacaaag agaaaagggc acaagcggga
     1801 attctgtgaa ttacccctca tacccttcca aagatgtatt gaacagtagc aacttttgca
     1861 actcattctt cacaggaaat aaaattttaa ccgccattgg cccaacaaaa aggcaaaaag
     1921 atccgtttag acgccttaaa cgcaaatata agcgatttaa gtattcaata tttttaatta
     1981 caaattgaaa aacaagggct caaccagtga tcaccggaaa tttatcgcaa gccggctttc
     2041 atgcatcgga taacctgaag gccaccgccc tgatgctgat gttttttctg ttttcgttta
     2101 catccgccgc ccaatacgat tcaactgtcg tgggccgcac gatgcagtct ttaaggaatg
     2161 tcaaaacgga tcgcgccgaa ggatattata tcacagaccc aggaaagcaa ggatggtttg
     2221 ttggtgatcc gggggacaca aagacccctg acgacagcgt gatgacgcta gtcgccgcaa
     2281 acggcatgcg gttcaagcgg accgataagg gcgatatcga cgtgagatgg ttcggagcga
     2341 ggggagacgg gaagtctgat gactggttct gcattcagaa agcgataacg ttcatcctgg
     2401 agcacaacac tgccagcagg acgctttctt tccctgccgg aacatattcc ctctccaggc
     2461 cactgatcat cgccagattt accggaaatt catacaaaca agccagcata aaccttgtag
     2521 gccccgcgaa ctcgaaaacc atatcggcag gctatgcggt ccttgcgccg actttcaaca
     2581 atacgtttgc catcggcgta caattgggt
//
LOCUS       DATGKE010000127         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1315505, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000127 DATGKE010000000
VERSION     DATGKE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315505"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VKU83_02240"
     CDS             complement(<1..498)
                     /locus_tag="VKU83_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92395.1"
                     /translation="MKTRLAFATLLLTLTAAHSYAQKSMEWGLKAGGNYFKVGGRSFD
                     NTYNISFSGGAYADLNYTSKFSLQPELLFNQVLCKTSQGFTQIYSNLQPVGFQLVSLD
                     YIALPVLLEYRPVPLVSVIIGPQYSYLIAQTQGLLRAETNENAFHHSELALVFGAELN
                     MGKTKA"
     gene            complement(654..1892)
                     /locus_tag="VKU83_02245"
     CDS             complement(654..1892)
                     /locus_tag="VKU83_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLI92396.1"
                     /translation="MRSWRYVWLIPILALVLDLYVFQAVKAVSQSASPRLRAGIYWSY
                     WVGSVLILVSLCLLPYLYRRYSWMQGYGFFILIGVYLSKLLIGIFLLLDDIRRVIQWI
                     AGRLMTTPAATHPASGDISRSVFMSWLGLGLGAGLYASLVHGFVNNYKYRVRRVRLNF
                     ANLPAPFKGLRILQISDIHSGSLKNPAAVRRGVDMALAEKPDIILFTGDLVNNLALEV
                     GDYQGIFGCLTAPLGVYSTLGNHDYGDYSWWENAEAKKANLDHLKRIQAEMGWRLLMN
                     EHVVLERGGEQIALIGVENWSAKARFPKYGKLDEAYAGAEKYPFKILMSHDPSHWDAQ
                     VRREYPDIDLTLSGHTHGMQFGLELPWFRWSPVQYIYKEWAGLYEEDRQKLYVNRGFG
                     FIGYPGRVGIMPEITVIELG"
     gene            2081..2890
                     /locus_tag="VKU83_02250"
     CDS             2081..2890
                     /locus_tag="VKU83_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI92397.1"
                     /translation="MAKIIGVANQKGGVGKTTSAINLAASLAVLEFRTLLVDADPQAN
                     STTGVGFDLHNVTQSLYDCMVNHIPVRDVILRSEIPHLDVVPSHIDLVGYEIEMINYP
                     NRESVLKGILEPVKDEYDFIIIDCSPSLGLITVNALTAADSVIVPVQTEFFALEGLGK
                     LLNTVKIVQSRLNPKLAIEGILMTMYDGRLRLCNQVVSEVRKHFDEIVFDTIIHRNTK
                     ISEAPSVGKPVILYDALSKGSVNYLNLAKEILQKNNLTRIKQEDRILDKQV"
     gene            2900..3820
                     /locus_tag="VKU83_02255"
     CDS             2900..3820
                     /locus_tag="VKU83_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLI92398.1"
                     /translation="MTTPHKKDKEALGKGIRSLLQSIDADLKTTSGELKSSVVEAVTN
                     MLRIPLEQIETNPRQPRHDFDEQGLQELAHSIRMHDIIQPVTVSKLPTGKYRLVSGER
                     RFRAARIAGLKDIPAYIRQTDDQQLLELALLENLQREDLNAMEIALSYKRMMEELNYT
                     QEQVAERMGKERSTVANYIRLLKLPPDIQVAVRSSQLSMGHARALINVDTVDKQLYLF
                     NEIKKKGLSVRQTEELVRQMYKENAAVKNSVKPGLPEAFKRIEDNLATHFSTRVKLSH
                     NKKGEGSISIEYYSLQELNKLLDQLGVSVN"
     gene            3830..>4364
                     /locus_tag="VKU83_02260"
     CDS             3830..>4364
                     /locus_tag="VKU83_02260"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HLI92399.1"
                     /translation="MNALRHLLVFTGFLFLHCVCRAQRTDTIPSRNDTIPAFIKGKTT
                     IPARPPDTVTPNSRPIRQKTTPYKVDSLARRQHDPRKATIRSAIIPGWGQLYNRKYWK
                     LPIVYAAVGIPAYAYFSNRAWYRKCQFALTILNNYQAAGFTGSQIPDTMLAKINVDLR
                     PLVAAGEDNSIRTARNEY"
BASE COUNT         1122 a         1250 c         1084 g          908 t
ORIGIN      
        1 agcctttgtt tttcccatgt tcagttccgc cccgaatacg agggcgagtt cgctgtgatg
       61 gaaagcattc tcgttcgttt cggcccgcag caacccctgt gtctgcgcga tgaggtaact
      121 gtactgcggt ccgataatga ccgacaccag cggcaccggc ctgtactcga gcaacaccgg
      181 caacgcgata taatccaggc tcaccaactg gaatcccacg ggctgcaaat tgctgtagat
      241 ctgggtaaag ccttgagacg ttttgcatag tacctggttg aacagcagtt ccggctgcag
      301 cgagaatttt gaagtatagt tcaaatcggc ataagcgccg ccgctaaaac tgatgttata
      361 ggtgttgtcg aaggagcggc ccccgacctt aaaataattg cccccggcct tcaaacccca
      421 ttccatagat ttttgagcat aagagtgagc ggcagtcagc gtcagtaaaa gggttgcgaa
      481 agctagtctg gttttcattg ttgatgcttg tttattgctt gatttatctc caattacaat
      541 gtgtgaaaat acagttcaat attctatcgg tttgttaaaa tcagcaaacc gcaagttaaa
      601 cggaacgccc ccgccccgca accctggtca ccaaagcgtc agcaacgaag gccttatccc
      661 aattcgatca ccgtgatttc cggcatgata cccacgcgcc cgggataacc gataaacccg
      721 aatccccggt tgacatataa tttctgcctg tcttcctcat acagcccggc ccattcctta
      781 taaatatatt gtacagggct ccaccggaac catgggagct ccagcccgaa ttgcattcca
      841 tgcgtatggc cggaaagcgt gagatcgata tccggatact cccttctcac ctgcgcgtcc
      901 caatggctgg gatcatggct catgaggatc ttgaatgggt atttttcagc ccccgcgtac
      961 gcctcgtcca actttccgta tttagggaaa cgcgccttcg cgctccagtt ctccacgcca
     1021 atcagcgcaa tttgttcgcc gcctctttcg agcacaacgt gctcattcat cagcaagcgc
     1081 caccccatct ctgcctggat ccgcttcaaa tgatcgaggt tcgccttttt cgcttccgcg
     1141 ttttcccacc aggaataatc cccgtaatcg tggttcccca gggtcgaata cactccgagc
     1201 ggcgccgtaa ggcagccgaa gatgccctgg taatcgccaa cctcgagcgc gagattgtta
     1261 accaggtcgc ccgtgaacag gataatatcg ggtttttccg caagggccat gtcgacgccg
     1321 cgccggacag cagcggggtt cttcaggctt cccgaatgaa tatcagatat ttgcaggatc
     1381 ctcaaaccct tgaacggggc aggcaggttg gcaaaattca ggcgcacgcg gcgaacccgg
     1441 tacttgtaat tattgacgaa cccatgcacc agggaagcgt acaaccctgc ccccagccca
     1501 agccctaacc agctcatgaa aaccgatcgt gaaatgtcac cggaggccgg atgtgtggct
     1561 gccggggttg tcatcaggcg cccggcaatc cattggatca cccgccggat gtcgtccagc
     1621 aacaggaaaa tcccaatcag gagcttggac agataaacgc cgatcaggat aaagaaaccg
     1681 tagccctgca tccagctgta acggcgatat aagtaaggca acagacaaag ggacaccagg
     1741 atcaggacag aacccaccca atagctccag tagatgccgg cccgaagccg gggcgaggcc
     1801 gactgggaaa ctgcttttac ggcctgaaaa acatacaaat cgagtaccag ggccaggata
     1861 gggatcaacc agacataccg ccagctacgc atgcaccaga aatttatatc tcataattta
     1921 tatcgaaggg ttaaatttgc catcctgtca gtgttcgtcc gggagacagg ccgcaaagtt
     1981 cggaattatc cccggtgggt aaaagaaaat tttgccgtaa ccccgattgg cgacaatttt
     2041 gacgaaattc gacaaaagcg ggaccgcaga caatcatatt atggcaaaaa ttattggagt
     2101 agcaaatcaa aaaggagggg ttgggaaaac tacgtccgcg atcaatctcg cggccagtct
     2161 tgccgtcctg gaattcagga ccctgctggt tgacgcggac cctcaggcga acagcacgac
     2221 cggcgtgggc tttgacctgc acaatgttac ccaaagcctg tacgactgca tggttaacca
     2281 cattccggtc agggacgtca tcctgcggtc ggaaatcccg catctcgacg ttgttccgtc
     2341 ccatatcgac ctggttggct atgagatcga aatgatcaat tatccgaacc gggagagtgt
     2401 cctgaagggg atcctggaac cggtaaaaga cgaatatgat tttatcatca tcgactgctc
     2461 cccttcgctt ggcctcatca ccgtcaacgc ccttaccgcc gccgactcgg tcatcgttcc
     2521 ggtccagacc gaattcttcg cactggaagg gctgggcaag ctgttgaata cggtcaagat
     2581 cgttcagagc cgcctcaatc cgaagctggc cattgaaggc atcctcatga caatgtacga
     2641 tggccgcctc cgcctttgca accaggtggt cagcgaggtc cggaaacatt tcgatgagat
     2701 cgtttttgac accatcatcc accggaatac caagatcagc gaagctccga gcgtcggcaa
     2761 gccggtgatc ctctacgacg cgctcagcaa gggatcggtc aactacctta acctggccaa
     2821 agaaattctt caaaagaaca acctgacccg gatcaaacag gaagacagga tcctcgataa
     2881 gcaggtttaa gctcaaaaca tgacaactcc ccataaaaaa gataaagaag cgttgggtaa
     2941 gggcatccgg tccctgttgc aaagcattga tgcggacctg aagacaacct ccggagagct
     3001 caagtcctcg gtcgtcgagg ctgtaacgaa tatgctcagg atccccctgg aacagatcga
     3061 gaccaacccg cggcagccgc ggcatgattt cgacgaacag gggttgcagg agctcgcgca
     3121 ttccatccgg atgcacgata tcatccagcc ggtgactgtc tcgaagctgc caacgggaaa
     3181 gtaccgcctg gtatccggtg aacgccgctt tcgggccgcc cgcatcgccg gcctcaaaga
     3241 catccctgca tatattcgcc agacggacga ccagcagttg ctggaactcg ccctcctcga
     3301 aaacctccag cgggaggacc tgaacgccat ggagatcgcc ttgagttaca agcggatgat
     3361 ggaagaactg aactacacgc aggaacaggt cgcggagcgg atgggaaagg aacgatccac
     3421 ggtcgctaac tatatccggc tgctcaaatt gccgcccgat atccaggttg cggtgcgcag
     3481 cagccagctc tccatgggcc atgcgcgggc cttgatcaac gtggacacgg tcgacaaaca
     3541 actctatctc ttcaatgaaa taaagaagaa aggcctttcg gtaaggcaaa cagaagagtt
     3601 ggtccgccag atgtacaagg agaatgccgc tgttaaaaat tcggtaaagc ccggccttcc
     3661 cgaggctttc aagcggattg aagataactt agctactcat ttcagcaccc gggtgaagct
     3721 cagtcacaac aaaaaagggg aaggcagcat ctcgatcgag tattattctc ttcaggagtt
     3781 gaacaaatta ctggatcagc tgggtgtctc ggttaactaa gcaagctcca tgaacgccct
     3841 caggcactta ctggtcttca caggctttct tttcctgcat tgcgtgtgca gagcgcaaag
     3901 gaccgacacg atcccttccc gtaacgatac tattccggct tttatcaagg gaaaaacaac
     3961 gatccccgcc agaccgccag acaccgttac gccaaacagc cggcccatcc ggcaaaaaac
     4021 gaccccctac aaagtcgatt cgctggcccg ccggcagcac gacccccgaa aagcaaccat
     4081 tcgctccgcg attatcccgg gctggggaca gctctacaac cgcaaatact ggaagttgcc
     4141 gatcgtttat gccgccgtcg gcattcccgc ctatgcctat ttctccaacc gcgcctggta
     4201 ccgcaaatgc cagttcgccc tcacgatcct gaataattac caagccgcgg gatttaccgg
     4261 cagccagatt cccgatacga tgctggccaa gatcaatgtt gacctaagac cgctcgtcgc
     4321 ggccggcgaa gacaactcca tccggacggc gcgaaatgaa tacc
//
LOCUS       DATGKE010000128         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_901035, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000128 DATGKE010000000
VERSION     DATGKE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901035"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VKU83_02265"
     CDS             complement(<1..614)
                     /locus_tag="VKU83_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI92400.1"
                     /translation="MHKRIIISVISDLVTDQRVHKVSLTLSEAGYTVLLIGARRKHSL
                     ELAPRSYSTRRIRMLAQKKAFFYAEFNLRLFLRLLFTRGDVFLANDLDALSAVWLAGK
                     LRRKPVVYDTHEYWLGMPELDGRPFVKKFWQRIENFIFPRLEHIYTICDSFAALYHKD
                     YGKKLGVVRNVPLLNAGPAMPLPPEVERRIPRDKFLLLFQGAGI"
     gene            complement(607..1686)
                     /gene="wecB"
                     /locus_tag="VKU83_02270"
     CDS             complement(607..1686)
                     /gene="wecB"
                     /locus_tag="VKU83_02270"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HLI92401.1"
                     /translation="MNIIHIVGNRPQFIKLSLLHGALSGHTRVRSRIIHTGQHFSENM
                     SDVFFRECGLPDPHHQLGIHSLSHNEMIGRMLIALDSALAVERPDAVVVYGDTNTTLA
                     ASLAAKKRQVRLFHVEAGVRTGDESMPEEVNRYLSDRMADLNFTCTALCSNNLVGEGM
                     PAARILDTGDLLLDASLRFADKARTLSTLPAALIPGGRPFILATIHRAANTGDPSALA
                     AILQALHELHEEYPVVFPVHPHTRAVIEANGLPFRLIDTPPLGYLDMLALIQASNYVI
                     TDSGGLSREAFFFSKPSVVVMDRPFWPEILATGASLAAPADSKTIIRCFRALSSSGKR
                     FHREVFGDGGAAQKIAGAILNHLHA"
     gene            1964..2236
                     /gene="rpsO"
                     /locus_tag="VKU83_02275"
     CDS             1964..2236
                     /gene="rpsO"
                     /locus_tag="VKU83_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLI92402.1"
                     /translation="MPYLTNDKRSSFFNNYGGSATNTGSIEGQIALLTERINRISEHL
                     QTNKKDFSTHRGLMQLVGKRKRLLTYLSKNNLQGYRQLIEKLGLRK"
     gene            2407..>2740
                     /locus_tag="VKU83_02280"
     CDS             2407..>2740
                     /locus_tag="VKU83_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92403.1"
                     /translation="MLSQPINVTFDTGEGRMVSIETGKLARQADGAVTVRQGNCIILA
                     TVVATREPKEGQDFFPLSVDYQEKFASAGRIPGSFFKREARLNDYEVLTSRLVDRALR
                     PLFPEDYFC"
BASE COUNT          641 a          685 c          826 g          588 t
ORIGIN      
        1 ttgatgccgg cgccctggaa caggagcagg aatttgtcgc gtgggatgcg cctttcgacc
       61 tctggcggca gcggcatggc cggcccggcg ttgagcaaag gtacgttgcg gaccacgccc
      121 agtttttttc cataatcttt gtggtagagg gcagcaaagg aatcgcagat cgtatagata
      181 tgctccagcc ggggaaaaat gaagttttcg atccgctgcc agaatttttt tacgaagggc
      241 cggccatcga gctccggcat gcccagccag tattcgtgcg tatcgtaaac gacgggcttt
      301 cgacggagct tgcccgccag ccaaaccgcg gacagggcgt caagatcgtt ggccaggaag
      361 acgtcgccac gggtaaagag caggcgcagg aaaagccgga ggttgaactc ggcgtagaag
      421 aaggcttttt tctgggcaag catgcgaatg cgtcgggtgg agtaggagcg gggagctaac
      481 tccaggctat gttttcgccg cgcgccgatc aggaggacgg tataacctgc ttcgctaagc
      541 gtaaggctga ccttgtgcac gcgctggtcg gtcaccaaat cggagataac ggaaatgatt
      601 attcgcttat gcatggagat ggtttaggat ggcgccggcg atcttttggg ctgcccctcc
      661 gtcgccgaag acttcccgat ggaaccgttt tccggaggag ctcaatgccc gaaagcaacg
      721 gatgatcgtt ttgctatcgg ccggggcggc caggctggcg ccggttgcca ggatctccgg
      781 ccaaaagggt ctatccatca cgacgaccga gggttttgaa aaaaagaagg cttctctgga
      841 aaggccgccg ctgtcggtga tcacatagtt gctggcctgg atgagggcga gcatgtcgag
      901 gtagcccagc ggcggggtgt cgatgagcct gaagggcagt ccgtttgcct cgatcactgc
      961 gcgggtatgg ggatggacgg gaaagacgac cggatactct tcgtgcagct cgtgaagggc
     1021 ctgaaggatc gcggccaggg cggaagggtc tccggtattc gcggcgcggt gaatggtcgc
     1081 caggataaag ggccggccgc cgggtatcag tgctgcgggg agggtggaca gcgtccgggc
     1141 cttgtctgca aagcgcaaag acgcatcgag cagcaggtcg ccggtatcca gaatacgggc
     1201 agcgggcatc ccttcgccca ccagattatt gctgcagagg gcggtacagg taaaattgag
     1261 gtccgccatg cggtcgctaa ggtagcggtt cacttcctcg ggcatcgatt cgtcgccggt
     1321 tcggacgcct gcttctacgt ggaaaaggcg gacctgccgt tttttggcgg ccagcgacgc
     1381 ggccaaagtg gtattggtgt ccccatagac gacgaccgca tcggggcgtt cgacggccag
     1441 ggcggagtcg agggcaatca gcatccggcc gatcatttca ttgtgcgaaa gggagtggat
     1501 gccgagctgg tggtgagggt ccggcagacc gcattcccgg aaaaagacat cggacatgtt
     1561 ttcgctgaaa tgctgccccg tatggatgat gcgcgagcgg acccgggtat gaccggaaag
     1621 ggcgccgtgc agaagggaaa gtttgatgaa ctgcggccgg ttgccgacga tgtgaatgat
     1681 attcatgaaa aaaatgcggc ggaacagccg cccgtgacgg ccttacgggc gaaaattacc
     1741 tatttaggcg gaaatgccta caaattttat ccgaaattgt caggttatta atattttatc
     1801 ttaattttgc cgcctgctaa agcagcagaa cctataggcg gcttccgggc ggccggccaa
     1861 acacccatcg accgctgtgc gccgggatgg gcgggccagg atgccaatcc ggtgtgcggg
     1921 gcggacaaat agcccgctga agataataaa aaaatagtta gagatgcctt atttgacaaa
     1981 cgacaaaaga tcgagttttt tcaacaatta tggcggaagc gccacgaaca ccggttctat
     2041 cgagggccag attgccttgt taacagagcg gattaaccgt atctccgaac acttgcagac
     2101 caataaaaaa gacttctcca cccatcgcgg tctcatgcaa ttagtgggaa aaagaaagcg
     2161 cctgcttacc tatctgagca agaataacct ccagggctac cgccagttga tcgaaaaact
     2221 cggattaaga aaataaaacg tgtatgttat aggatcacta ttcccaactg atcggccggt
     2281 tgggaatcgt ttttttatag ccaaacgatt tgtagtagtt tatttagatt ttttaacata
     2341 gaatttcggt ttatttatcc gtcccccctc aagtaagccg caattcgaca aagacgcaga
     2401 aaactcatgc tttcacaacc tattaatgta acgtttgata ccggtgaagg ccgcatggtc
     2461 tccattgaaa ccggaaagct tgcccgtcag gctgacggag ccgttaccgt acgccaggga
     2521 aattgcatca ttctagccac ggttgttgcc accagggaac cgaaggaagg gcaggacttc
     2581 ttcccgctgt ccgttgatta ccaggaaaaa tttgcatctg ccggtcgcat ccccggctcg
     2641 ttcttcaaac gtgaggccag actgaatgat tacgaggtcc tgaccagccg cctcgtcgat
     2701 cgcgcgcttc ggccgctttt tcccgaagac tatttttgtg
//
LOCUS       DATGKE010000129         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1442386, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000129 DATGKE010000000
VERSION     DATGKE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442386"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VKU83_02285"
     CDS             complement(<1..497)
                     /locus_tag="VKU83_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLI92404.1"
                     /translation="MADIFEKLVKHNGPIGQHRERAHGYFAFPKLEGEIGSRMMFRGK
                     EKIVWSLNNYLGLANHPEIRKVDAEAAARYGMAAPMGARMMSGNSSFHEQLEKELAAF
                     EKKEDATLFNYGYQGIMSAIDAICGRHDVIVYDAESHACIIDGLRLHPGHRYVFKHND
                     IEDCE"
     gene            780..1106
                     /locus_tag="VKU83_02290"
     CDS             780..1106
                     /locus_tag="VKU83_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HLI92405.1"
                     /translation="MITVSPRAKEYISQLMADQHASPQTFVRVGVKSGGCSGLEYKLD
                     FDNQAREGDQVFEDKGIRVVVDMKSLLYLYGTELDYSGGLNGKGLTFNNPNAARTCSC
                     GESFSV"
     gene            1148..2593
                     /gene="sufB"
                     /locus_tag="VKU83_02295"
     CDS             1148..2593
                     /gene="sufB"
                     /locus_tag="VKU83_02295"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HLI92406.1"
                     /translation="MANDNEIIDNIANKEYEFGFVTDIEMDVAPAGLNENTIRFISEK
                     KKEPEWLLDWRLKGLQAFGRQKLPDWQNFELPPIDFQKISYYAAPKNKKKYDSLDQVD
                     PELLATFEKLGIPLSEQKALAGVAVDVVFDSVSVKTTYKEKLKELGIIFCSFGEAVQE
                     HPDLVKRYLGSVVPYSDNIFAALNAAAFSDGSFVYIPKGVRCPMELSTYFRINTENTG
                     QFERTLIIADEGSYVSYLEGCTAPMRDENQLHAAVVELIALENAEIKYSTVQNWYPGD
                     KDGKGGIYNFVTKRGICKGANAKISWTQVETGSAITWKYPSVILQGDNSTGEFYSVAV
                     TRNKQIADTGTKMHHIGRNTRSRIISKGISAGQGQNSYRGLVQIGPKADAARNFTQCD
                     SLLIGDRCGAHTFPYIESKNNSATVEHEATTSKIGEDQIFYLNQRGIDSEKAVALIIN
                     GYAREVLNQLPMEFAVEAQKLLSITLEGSVG"
     gene            2602..>2782
                     /locus_tag="VKU83_02300"
     CDS             2602..>2782
                     /locus_tag="VKU83_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI92407.1"
                     /translation="MNKEMLIIKNLHAEVEGKQILKGIDLKVNAGEVHAIMGPNGSGK
                     SSLAAVLAGREDYDVT"
BASE COUNT          699 a          680 c          777 g          626 t
ORIGIN      
        1 ttctcacagt cttcgatatc gttgtgcttg aagacgtacc ggtggccagg atgaagccgg
       61 aggccgtcga tgatgcaggc atggctttcc gcatcataaa cgatcacgtc atgccggccg
      121 caaatggcat cgattgcgct cataatccct tgatagccat aattaaacaa agtcgcgtct
      181 tcctttttct caaaggcggc cagctccttt tccaattgtt cgtggaaact gctgtttccg
      241 ctcatcatcc gggcccccat cggcgcggcc atcccgtacc gggcagcggc ttcggcgtcg
      301 acctttcgga tttcggggtg gttggcaagc ccaagatagt tgttaaggct ccaaacaatc
      361 ttctctttcc ccctgaacat catcctgctg ccaatctctc cctccagttt cggaaaggcg
      421 aaatacccgt gcgctctttc ccggtgttga ccgatcggtc cattatgctt cactaacttt
      481 tcaaaaatgt ctgccattgt tttgaaataa ttaaagttat gttgcccgca aatttaacgt
      541 atttggcgat tcggcggccc attgtttgac cggcgggtca attttaacgg cgcgactgac
      601 acctgggcag aacccccggg ccgtgatccg ccgcgcggcg cggcggacac gacattgcta
      661 aaaaactagg aattccgcct tattttgcgg aattttgcag ggcaggaatt tccaatctcg
      721 caaccgggcg agggaacagt tccgggcatg aattgttcta ctatataaaa atttgaatga
      781 tgatcaccgt ttcaccaagg gcgaaagagt atatcagcca attgatggcc gaccagcatg
      841 cgtccccgca aacctttgtc agggtagggg tgaaaagcgg cggctgttca gggctcgaat
      901 acaagctgga ttttgacaac caggcacggg agggggacca ggtctttgaa gacaaaggca
      961 tccgcgtcgt ggtggacatg aagagcctgc tgtatttgta tggcaccgag ctggattatt
     1021 ccggcggcct gaatggtaaa gggttgactt tcaataaccc gaatgctgcc aggacctgca
     1081 gttgcgggga aagcttttcc gtttagcggg ggtccggtaa tatcaaacta agttatcttc
     1141 gtgcattatg gccaacgaca acgaaataat tgacaatata gccaataagg agtatgaatt
     1201 cgggttcgtg acggacatcg aaatggatgt ggcgccggct ggcctcaatg aaaatacgat
     1261 ccggttcatt tccgagaaga aaaaagagcc ggaatggctg ctggactggc ggctgaaggg
     1321 ccttcaggca ttcggaaggc aaaagctgcc cgactggcag aacttcgagt tgcccccgat
     1381 cgatttccag aaaatatcct attacgccgc tcctaagaat aagaagaagt atgacagcct
     1441 ggaccaggtg gacccggagc tactggcgac gtttgaaaaa ctgggtattc cgctttccga
     1501 acaaaaggct ctcgcggggg tggcggtgga tgtggtattt gacagcgttt cggtcaaaac
     1561 gacctataag gagaaactca aagagctggg aatcattttc tgctccttcg gcgaagcggt
     1621 acaggagcat cctgatctgg tgaagagata tctcggatcg gtagtaccct actcggacaa
     1681 tatctttgcc gccctgaatg ccgcggcatt ttcagacggc tcttttgtct atatcccgaa
     1741 aggcgtccgc tgtccgatgg agctctcgac ctatttccgg atcaatacgg aaaataccgg
     1801 ccagttcgaa cggaccctga tcattgcgga tgagggcagc tacgtcagct acctggaggg
     1861 ctgcacggcg ccgatgcggg atgagaacca attacatgcg gcggttgtcg aactcattgc
     1921 cctggagaac gccgagatca aatattccac cgtgcagaac tggtatcccg gggacaagga
     1981 tgggaagggc gggatctaca attttgtgac caagaggggt atctgcaagg gcgcgaatgc
     2041 aaagatctcc tggacccagg tcgagaccgg atcggcgatc acctggaaat atcccagcgt
     2101 gatccttcag ggcgacaatt caacgggcga attctattcg gttgcggtga cccggaataa
     2161 gcagatagcg gataccggca cgaagatgca tcacatcggg cggaataccc ggagccggat
     2221 catttccaaa ggaatctcgg ccggacaggg acaaaacagc taccgcggcc tggtgcagat
     2281 cgggccgaag gcggacgccg cacgtaattt tacgcagtgc gactccttgc tgatcggcga
     2341 tagatgcggt gctcatacgt ttccttatat cgagtcgaaa aataacagtg ctaccgtgga
     2401 gcatgaggcg acgacttcga agatcgggga ggaccagatc ttttatctca atcagcgcgg
     2461 gatagactcg gaaaaagcgg tcgccctcat catcaacgga tatgcgcggg aagtgctgaa
     2521 tcagcttccc atggaattcg cggtggaagc gcagaagtta ttgtcgatta cattggaagg
     2581 gagtgtcgga taaaaaacaa gatgaataaa gaaatgctga tcattaagaa tttacacgcc
     2641 gaagtggaag gaaagcagat ccttaagggg attgatctga aggtgaatgc aggcgaagtg
     2701 cacgctatca tgggaccaaa cggctctggc aagagttcac ttgccgccgt gctggccggc
     2761 cgggaggatt atgatgtcac cc
//
LOCUS       DATGKE010000130         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_593577, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000130 DATGKE010000000
VERSION     DATGKE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593577"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VKU83_02305"
     CDS             <1..541
                     /locus_tag="VKU83_02305"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLI92408.1"
                     /translation="CDIFQPKAIARICRIKQVDPAKARLSFICHDLSDLSNYTKSIST
                     PLYRLLRGRLPGPYTFILPASKQVPKILKSRKDTIGLRIPDNVIARSLVRELGHPILS
                     SSLPGGILAGSADDPAEVLRVEEYTDPERINEKFGNLVDIVVDGGIGGILPSTIIDCT
                     GEEPELVRKGLGPWEEVHS"
     gene            complement(501..1118)
                     /locus_tag="VKU83_02310"
     CDS             complement(501..1118)
                     /locus_tag="VKU83_02310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLI92409.1"
                     /translation="MDNPLRTHYQDELTEAGCDEAGRGCFAGPVFAAAVVLPPGFFHP
                     LLNDSKQVTPENRNLLRGYIEANAVAWSVARVDEDEIDRINILRASFKAMHLALDRLK
                     IRPKLLLIDGNRFTRYRRTAHRCIIKGDSIFASIAAASILAKTWRDEYMLNLHSEYPE
                     YGWITNKGYGTREHRDAIGKFGLCKYHRKSFDIMSEPLPMDPDLF"
     gene            1176..2279
                     /locus_tag="VKU83_02315"
     CDS             1176..2279
                     /locus_tag="VKU83_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLI92410.1"
                     /translation="MSTATFPQRRGGLVAIWIYIGVAMLLVQVILGGITRLTGSGLSI
                     TEWDVAAGVVPPLNAAQWQEAFDKYRQTPQFHLVNSGFTIADFKFIFFWEWFHRFWAR
                     MVGVVFLVGFAWLLIKKQMGREMVRPLIVLFLLGALQGVVGWIMVASGLVGDAIYVAP
                     TRLAMHFIFALVLVVYTLWLGLQLSVPPDLRLARAGDRASIASLRRWTVAILAVLVFQ
                     LIYGALMAGHKAANVAPTWPDINGAFIPRGMFDRSFVESAIGNRITVQFIHRTLAYIL
                     LLLVLVWTVKAASLKAVPAAFRSLQWLPLLLIAVQILLGISSLLTSPWIRPHHWVAFD
                     WLAQFHQITGLLFLLTMVGMLYLVFPVRRSVMV"
BASE COUNT          462 a          649 c          691 g          581 t
ORIGIN      
        1 ctgcgatatt ttccaaccta aggctatcgc ccgcatctgc cggatcaagc aggtcgatcc
       61 cgccaaagcc cgcttatcgt tcatctgtca cgacctgagt gacctgagca actatacaaa
      121 gagcatttcc acgccgctgt accggttgct gcgaggccgg ctgcccgggc cgtacacctt
      181 tattttgccg gcaagcaaac aggtcccaaa aatattgaag agccggaaag acacgattgg
      241 actgcggata ccggacaatg tgatcgcccg ttcgctggtc cgcgagctgg gtcatcccat
      301 cctcagctcc tccctgccgg ggggaatcct ggccgggtct gcggatgacc cggcggaggt
      361 gctaagggtc gaagaatata ccgatccgga aaggatcaat gaaaaattcg gcaaccttgt
      421 ggacatcgtg gtggacggtg gcatcggcgg catccttcct tccacaatca tcgattgcac
      481 cggcgaggag ccggaacttg tcagaaaagg tctgggtcca tgggaagagg ttcactcatg
      541 atatcgaaac ttttccggtg gtatttgcag agcccgaatt tcccgatggc atcgcgatgt
      601 tccctggtgc cgtaaccctt attcgtgatc cagccgtatt ccggatattc ggaatggagg
      661 ttcagcatgt attcatcccg ccaggttttg gcgagaatgc tggcggctgc gatggaggcg
      721 aagatcgaat cgcccttgat gatgcaacga tgggcggttc gccggtagcg tgtgaaccgg
      781 ttgccgtcga tgagcagcaa ctttggccgg atcttcagcc ggtcgagggc aaggtgcatc
      841 gccttgaacg aggcccggag aatattgatc cgatcgatct cgtcctcatc cacccgcgca
      901 acgctccagg ccacagcatt agcctcgatg tatccgcgca gcaggttccg gttttcaggg
      961 gtgacttgtt tggaatcatt cagcagcgga tggaaaaaac ccggcggcag gactaccgca
     1021 gcggcaaata ccggtcctgc aaaacaaccg cggccggcct catcgcaacc ggcttccgtc
     1081 agctcgtcct ggtaatgcgt tctcagcggg ttgtccatgc gccaaaaata agtattcagg
     1141 gcattaaata atcattagtg actaaatttg ccgccatgtc aacggctact tttccccagc
     1201 gccgcggagg gctcgtggca atctggatct acattggggt cgcaatgctg ctcgtgcagg
     1261 tcatactcgg cggcatcacc aggcttaccg gatcgggtct ttccatcacc gaatgggatg
     1321 tcgcagcagg cgtggtgccg ccgctgaatg ccgcgcagtg gcaggaggct ttcgacaaat
     1381 accgccagac gccgcaattc catctggtca attccgggtt cacgattgcc gatttcaaat
     1441 tcatcttttt ttgggaatgg ttccaccgtt tctgggcccg catggtcggc gtggttttcc
     1501 ttgtgggatt tgcctggctg ctgataaaaa agcaaatggg ccgggagatg gtccggccgc
     1561 tgatcgtcct gttcctgctg ggcgcgttgc aaggcgtggt aggatggatc atggttgcca
     1621 gcggccttgt aggcgacgcc atctatgtcg caccgaccag gctggcgatg cactttatat
     1681 ttgcgcttgt attggttgtt tatactttgt ggcttgggct gcagctttct gtgcctccgg
     1741 acctgcgatt ggcccgcgca ggggaccggg cctccatcgc gtcgctccgc cggtggacgg
     1801 tcgccattct ggccgtgttg gtttttcagc tgatatacgg tgcactcatg gcggggcata
     1861 aggccgcgaa cgtggccccc acctggcccg atatcaacgg cgcatttatt ccccggggga
     1921 tgttcgacag gtccttcgtg gagtcggcga tcggcaaccg gataaccgtt cagttcatcc
     1981 atcggaccct tgcgtatata ttgttgctgc tggtgctggt ctggaccgtc aaggcggcaa
     2041 gcctgaaagc ggttccggcc gctttccggt cattgcagtg gttgccgctg ctattgatcg
     2101 cggtacaaat cctgctgggt atcagcagcc tgctgaccag tccgtggatc aggcctcacc
     2161 attgggtggc gtttgactgg ctggctcagt tccaccagat cacgggcctc ttattcttgc
     2221 tgacaatggt gggaatgtta taccttgtgt tcccagttag acgttctgta atggtatgat
     2281 gcgaatccta aaagccattt actacttttt cccggttcaa ttgctgttgg ggcaactcag
     2341 aaggcatcag gtgttgctga tcttttgggc cattctgttc agt
//
LOCUS       DATGKE010000131         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1002368, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000131 DATGKE010000000
VERSION     DATGKE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002368"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VKU83_02320"
     CDS             <1..1136
                     /locus_tag="VKU83_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92411.1"
                     /translation="PAQLKTIDPVVSPDGRYVYFSRRFGPWSYNAGLPQYQIGVYDRQ
                     NSQISTLTSRYGSSFTPVLSKDGKWLVYGSRFEDKTGLVIRNLATGDEHWLAYPVQRD
                     DQESIATMGVLPGMAFTPDSKAVIAAYGGKIHRIPIDGGKPAEIPVTVDLQLELGPRL
                     EFKYPVSDSAYGLASQIRDAVPSPDGKKLAFTVLDRLYVMDYPKGTPKRLTDHDFIEA
                     QPNWSRDGKTLVFVSWSTQGGQIYKVAATGGPVQQLSKAPAVYQNPAFDNTGNRIVFL
                     RSPAQKYKEAFETGYDDGEEDLCWVPAAGGDIAVITKAHGRANPHFVQGEDRIYLNNG
                     NGDLLSINWLGTDEKTIVHITGITTYGISVFKHGRPDYGSEDN"
     gene            1164..2921
                     /locus_tag="VKU83_02325"
     CDS             1164..2921
                     /locus_tag="VKU83_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI92412.1"
                     /translation="MEVQQASAAAIITMSPTGKQAIAQINNEIYMATIPETGKTVNIS
                     VADAGSAQFPARKLTDLGGEFPFWEADGKKVHWSLGNAHFVYDLDASQAFDDSVKLAK
                     KAKADSLAKAAAAPKKDSAAKDSVGGARKDSAAAKKDNAAPKKPATYKAIETAVNVYY
                     ERDEPKGVVLLKGARIITMEGNEVIENGDILVENNRIKGIGPTGSLSVPSQAKVIDVS
                     GKTIVPGFVDTHSHMWPQWGLHKSQVWKYAMNLAYGVTTTRDPQTATTDIMTYRDEVD
                     AGAVFGPRIYSTGPGVGFWDYNVKDSAQAEDILRQYSKYYNTHYIKMYLTGNRQQREW
                     IIMAARNQQLMPTTEGGLNTKLNICNLLDGYPGHEHAIPIYPLYNDLIKTIAFSQMCV
                     TPTLIVSYGGPFAENYWWETENPYHDAKVQYFMPYEEVAGKTRRDKAGWFMPEEQVFP
                     KHAKSMKSMVEAGALAGIGSHGEFQGIGYHWEMWAMGSGGMKPMDVLRVATILGATGL
                     GLDKDLGSLKTGKLADLVILEKNPLDNLRNTNSVYYVMKNGRLYDGNTCNELYPGKRV
                     LDRSEWSFEKPAKTTDVPE"
     gene            2960..>3311
                     /locus_tag="VKU83_02330"
     CDS             2960..>3311
                     /locus_tag="VKU83_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92413.1"
                     /translation="MLFICLLAALGTAAQTCTGGLGDPIVDITFGQGAVDAGPLKGGI
                     TNLTFEPSDCPNDGYYTIAPRSSYCFGNTWWTVSQDHTGNIDGNFMLINASYLPSVFF
                     VREVSGLCGGTSYQF"
BASE COUNT          787 a          766 c         1086 g          672 t
ORIGIN      
        1 ccccggcgca gttgaagacg atcgatccgg tagtgagccc ggatggccgg tacgtctatt
       61 tttcgcggcg tttcggtccg tggtcgtata atgccggttt gccgcagtat cagatcggtg
      121 tctatgaccg gcagaattcg cagatcagca cactgacctc gcggtatgga agcagtttca
      181 cgccggtgtt gagcaaagac ggaaagtggc tggtctatgg cagccggttc gaggataaga
      241 caggtctggt gatccgtaat ctggcgacgg gagatgagca ttggctggcg tatccggtac
      301 agcgggacga tcaggagtcc atcgccacga tgggggtatt gccgggcatg gccttcacgc
      361 ccgacagcaa ggcggtgatc gcggcttatg gcggcaagat ccaccggata cccatcgatg
      421 gcggcaagcc ggcggagatc cctgtaacgg tagatctgca gctggaactg gggccgcggc
      481 tcgaattcaa atatcctgtg agcgattcgg cctatgggct ggcgtcgcag atccgggatg
      541 ccgtcccttc gccggatgga aagaaattgg cgtttacggt gctggaccgg ctttacgtaa
      601 tggattatcc caagggtacg ccgaaacggc tgaccgatca tgattttatc gaggcgcagc
      661 cgaactggag cagggacggc aaaacgcttg tttttgtaag ctggtcgacg cagggcgggc
      721 agatctataa agtcgccgcg accggcgggc cggtacagca actctcgaag gcgccggcgg
      781 tatatcagaa tcctgcgttc gacaataccg gcaaccgcat cgtcttcctg cggagcccgg
      841 cgcagaaata caaggaggca ttcgaaacgg ggtatgacga tggggaagag gatctgtgct
      901 gggtgcctgc agcgggtggg gatatcgccg tgatcaccaa ggcgcatggc agggccaatc
      961 cgcatttcgt gcaaggcgag gaccgcatct atctgaataa cggaaatggc gatctgctgt
     1021 ccatcaactg gctgggaacg gatgagaaga cgatcgtaca tatcacgggg atcactacct
     1081 atggcataag cgtattcaag catgggcgtc cggactacgg ctccgaggat aactgactgc
     1141 tgagcgagac ggctgcagaa gcaatggagg ttcagcaggc cagcgcggcg gcgataatca
     1201 cgatgtcgcc gacggggaag caggcgatcg cgcagatcaa taatgagatc tacatggcga
     1261 cgataccgga gacggggaag acggtgaata tatcggtggc cgatgcgggg agtgcccaat
     1321 ttccggcgcg gaaactgacc gatctgggcg gggaattccc tttttgggaa gccgatggca
     1381 aaaaagtgca ctggagcctg ggaaatgcgc attttgtgta tgatctggat gcctcgcagg
     1441 cgttcgacga cagcgtaaag ctggcgaaga aggcgaaggc cgatagtctc gccaaggcgg
     1501 ctgccgcgcc gaaaaaggat agtgcggcaa aagacagtgt cggcggggcc aggaaggaca
     1561 gtgcggctgc gaaaaaggat aatgcagcgc cgaagaagcc ggctacgtat aaggcgatcg
     1621 agacagcggt gaatgtttat tatgagcggg atgagccgaa gggggttgta ttgctgaaag
     1681 gagcgcggat catcacgatg gaaggcaatg aagtcatcga gaatggggat atacttgtcg
     1741 agaacaaccg cataaagggg atagggccga cgggtagtct atctgtaccg tcgcaggcca
     1801 aagtgatcga tgtctccgga aagacgatcg tgccgggctt cgtggacacg cattcgcata
     1861 tgtggccgca gtgggggttg cataagagcc aggtatggaa atacgccatg aacctggctt
     1921 atggcgtaac gaccacgcgc gatccgcaga cggccacgac ggatatcatg acctatcgcg
     1981 atgaagtgga tgcgggtgcg gtgttcgggc cgcggatata cagcaccggg cctggtgtag
     2041 gattctggga ctacaacgtg aaggactccg cgcaggcaga agatatcctg cgccagtaca
     2101 gcaaatatta taatacgcat tacattaaga tgtatctcac gggcaaccgg cagcagcggg
     2161 aatggatcat catggcggcg cgtaatcagc agctaatgcc gactaccgaa ggcgggttga
     2221 ataccaagct gaatatctgc aatctgctgg atggctatcc gggtcatgag catgccatac
     2281 ctatttatcc gctctacaat gacctgataa agacgatcgc tttttcgcag atgtgtgtaa
     2341 cgcccacgct cattgtgtcc tatggcggac catttgccga gaattactgg tgggagacgg
     2401 agaacccgta tcatgacgcg aaggtgcagt atttcatgcc gtatgaagaa gttgccggca
     2461 aaacgcgccg ggacaaggcc gggtggttca tgccggaaga gcaggtcttt cccaagcatg
     2521 ccaagtcgat gaagtcgatg gtcgaagcgg gggcattggc gggtatcggt agtcatggcg
     2581 aattccaggg gatcggttat cattgggaga tgtgggccat gggttccggg gggatgaagc
     2641 cgatggatgt gctgcgtgtg gctaccatat tgggggccac gggattaggc ttggacaagg
     2701 atctaggcag tctcaagacc gggaagctgg ccgatctggt gatcctggaa aagaatccgc
     2761 tggacaacct aagaaacacg aattccgtat actatgtgat gaagaatggg cggctatacg
     2821 atgggaatac ctgcaatgag ctctatccgg gcaaacgggt gttggacagg agcgagtgga
     2881 gttttgagaa accggcgaaa accacggatg tgccagagta ggggtttgag tgttttagta
     2941 ctattgcatg cggaaattga tgctgttcat atgcttgctg gctgccctgg gaacggcggc
     3001 gcagacctgt acgggggggc tgggcgaccc cattgtggat ataacgttcg gtcagggagc
     3061 ggtggatgcc ggtccattga aaggtgggat caccaatctt acttttgagc cgtcggattg
     3121 tccaaacgat ggatactaca ctatcgcgcc ccgaagctcc tactgttttg gcaacacctg
     3181 gtggacggtg tcgcaggatc atacggggaa tatcgacgga aatttcatgt tgatcaacgc
     3241 ttcttacctg cccagtgtct tttttgtaag ggaggtgagc gggctttgcg gcggcacgag
     3301 ctaccagttc t
//
LOCUS       DATGKE010000132         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_277744, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000132 DATGKE010000000
VERSION     DATGKE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277744"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VKU83_02335"
     CDS             complement(<1..973)
                     /locus_tag="VKU83_02335"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HLI92414.1"
                     /translation="MSYQPIENYGIIGDLNTTALVGLNGSIDFMCFPRFDSPSLFAAL
                     LDDKKGGNFRISPGFKEMKTKQLYLPDTNVLLTRFLSSEGVAEVVDFMPVDPNGEKRL
                     IRRVTTVRGEVKFQLRCCPRFEYGRKAHRVVSTGDQEITFQVEDAPDLSVRLKASIPL
                     STAGADAVAEFTLQAGESAAFLLESAANEEARDQDLMEMVAGSLARTINYWKDWMSRS
                     TYRGRWMEVVNRSALVLKLLSSCEFGSIVAAPTFGLPESIGGKRNWDYRYTWIRDASF
                     TVYSLIRLGYTKEAGAFMNWVERLFRKIKEDDRLGIMYSIDGTRQLQE"
     gene            1064..1861
                     /locus_tag="VKU83_02340"
     CDS             1064..1861
                     /locus_tag="VKU83_02340"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLI92415.1"
                     /translation="MKKHTYLRLFFLAAALVSMALAQFSFFSGSKFIFSSHLAGHVTL
                     LLITAPFSVLAMRERPEAKGGLAFGLSRWLSAMPWLNWILGIGILWLWQVPSVFNALI
                     TWDGPGFHRHLHLLSFLHSGSLLLAGILFAWPLAGPYLSHRLSAPESLLYITTAWLGI
                     SLLAFLIEFASPGLYGSPALPIAHKDQVTAGWVLWLPSCVVYLFGGILLLREWMGGEL
                     WIHHEFYRREKRRGTRTVHSADQGPAETGGFVPRGGAIIPHHSPQQI"
     gene            complement(1917..3008)
                     /locus_tag="VKU83_02345"
     CDS             complement(1917..3008)
                     /locus_tag="VKU83_02345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLI92416.1"
                     /translation="MQETQHLAAIIIDKIKTGGPMPFREFMELALYHPRLGYYTSVRE
                     QIGKRGDFFTSPCLTGLFGDLLGAQFEEMWRLLGGVPFTIVEIGAGTGVLSRDILSRL
                     KSVPEMYRKLNYFIIEKSETMRRRERHLLPEKVSWRGSLGEVGAVTGCIFSNELVDNF
                     SVHRVVMGEELMEVYVDFESGFREVLQPASDELKAYFAALGVALAPGQEAEINLEATA
                     WIQEVATTLRRGWVVTIDYGHTASDLYSRKSGTLACFHRHRVHHCPYDFIGEQDITSH
                     VNFTALDHWGRTQGLEVCGYTNQTHFLQGLGLAGRLRQWEEMGSGDRDMSGMRTFLLE
                     MGQKFKVLIQQKGVGRSFLSGLQFARVLG"
     gene            3098..>4373
                     /locus_tag="VKU83_02350"
     CDS             3098..>4373
                     /locus_tag="VKU83_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92417.1"
                     /translation="MSSTTSLPQQDAPGASRGDLEQLCINTIRVLSADAVQKANSGHP
                     GTPMGTAPMGHVLWSEVMRYNPRDPAWPNRDRFVLSAGHACMLQYCYLFLTGYDLSLD
                     DIRHFRQLHSKTPGHPEYGLTPGIEVTTGPLGQGFANSVGMAIAQRHVAARFNRPGFD
                     LFNYRIYGICSDGDMMEGVASEAASMAGHLGLGQIIFLYDDNHISIEGNTNVTFSEDV
                     GKRFEAYGWHVQVLEDGNDTGAIRQAIQRAQAETTRPSLIKVRTHIGYGSPNKVDTGS
                     AHGSPLGADEVKLVKKGFGFDPDASFVVPEQVLAFYRKAGEKGTAAEKQWTALWESYR
                     QQFPDLAAEWEAMRQEQLPGDWEKKVPVFRQSDGKDGQISTRVAAGKVLNSIAPLFPH
                     LIGGSADLAPSTETNLKNFSSFSADDPGGRNFH"
BASE COUNT          910 a         1343 c         1161 g          959 t
ORIGIN      
        1 actcctgtaa ctgccttgtc ccgtcaatgg aatacatgat gcccaaacgg tcgtcttcct
       61 tgatcttcct gaacagcctc tcgacccaat tcatgaaggc gccggcctcc ttcgtatatc
      121 ccagccggat aagcgaatat accgtaaagg acgcgtcgcg gatccaggtg taacgataat
      181 cccagttccg tttgccgccg atgctttccg gcaacccgaa cgtgggagcg gcaacgatcg
      241 acccgaattc acaggaagac aataatttca ggacgagggc cgaacgattg acgacctcca
      301 tccatcttcc ccggtaggtg ctgcgggaca tccagtcctt ccagtaattg atcgttcttg
      361 cgagactgcc ggcgaccatc tccatgaggt cctggtcccg ggcttcctcg ttcgcggcgc
      421 tctccagcaa aaacgctgcc gattccccgg cctgaagggt aaattctgcc acagcgtcgg
      481 caccggcagt acttagcgga atggaggcct tcagccttac cgacagatcc ggggcatctt
      541 caacctggaa agtaatctcc tggtcgccgg tggaaacaac tctgtgggcc tttcttccgt
      601 attcaaagcg gggacagcac cgtaattgaa attttacctc gccccggacc gtggtcacgc
      661 ggcggatgag ccttttctca ccgttcggat ccacaggcat aaaatcgacg acctccgcaa
      721 ccccttccga ggagaggaac cgagtcagca gaacgttggt gtccggcaaa tacaattgtt
      781 tggttttcat ttccttaaac cccggactga tccgaaagtt gcctcccttt ttgtcatcca
      841 ggagcgctgc gaagagactg ggggaatcga atcgtgggaa acacatgaaa tcgatggaac
      901 cgttgagacc gaccagggct gtagtgttga gatcgccgat tatcccgtaa ttctcgatcg
      961 gttggtagct cacgatgttt aatttaatag cggggcaaaa accattcctc aaggttggca
     1021 gaaattttgt ataaacctta ttgtcttttt atcacaaccc atcatgaaaa aacatactta
     1081 tctccggctt ttctttttag cagcagcttt ggttagtatg gctctggccc agttctcctt
     1141 tttttccggc agtaaattca tcttcagctc gcatctggcg ggccatgtca ccctgctgct
     1201 catcaccgcg ccattttcgg tactggcgat gagagaacgc cccgaagcca agggtggcct
     1261 tgctttcggc ctttcccgct ggctttcggc catgccctgg ctgaactgga tcctgggaat
     1321 cggcatccta tggctctggc aagtgccttc tgtcttcaat gcgctgataa cctgggatgg
     1381 ccccggcttt catcgccacc ttcacctgct ttcctttctg cattccggta gcctgctgct
     1441 ggcagggatc cttttcgcct ggccgcttgc ggggccctac ctttcacacc gtctgtcggc
     1501 gcccgaatcc ctgctttata ttacgacggc ctggctgggt atcagccttc tggctttcct
     1561 tatcgaattc gcctcgcccg gactgtatgg atccccggcc cttcccatcg cccataagga
     1621 tcaggtgacc gccggatggg tgctttggct ccccagctgc gtcgtttatc tcttcggggg
     1681 aattctcctg ctgcgggaat ggatgggcgg cgaactctgg atccaccacg agttctatcg
     1741 ccgcgaaaaa cgccgcggga cccggaccgt ccacagcgcg gaccaggggc cagccgaaac
     1801 gggcggattt gtccctcgcg gcggggccat tattcctcac cattcgccac agcagattta
     1861 gccggtggct gggcaatgcc cttttccagc gccctgaaag tcgccagtgt ggcatttcat
     1921 cccaacaccc gggcaaactg tagcccggaa agaaagctcc tgccgactcc cttttgctgg
     1981 atcaggacct taaacttttg gcccatctca agaagaaagg tcctcatgcc gctcatgtcc
     2041 cggtcgccgg aacccatctc ctcccactgc cgcaaccgtc cggccaatcc cagcccctgc
     2101 aagaaatggg tttggttggt atagccgcaa acttccaggc cctgcgttct gccccaatga
     2161 tccagcgcgg taaaattcac gtggctggtg atgtcttgtt ccccgataaa gtcgtaggga
     2221 cagtgatgca cccggtgtct gtggaaacat gccagcgttc cggacttcct gctgtacaga
     2281 tcggaggcgg tatggccgta gtcgatcgtc actacccacc ctctgcgcaa ggtggtagcc
     2341 acttcctgga tccacgcggt cgcctccagg ttgatctcgg cctcctgacc gggcgccagc
     2401 gccacgccaa gggcggcgaa atacgctttc agctcgtcgg acgcgggctg cagcacttcc
     2461 cggaacccgc tttcaaagtc cacatagact tccatcagct cttcgcccat gacaacgcgg
     2521 tgtacagaaa agttatccac cagttcgttg gaaaagatgc agcctgttac cgctccgacc
     2581 tccccaagcg accccctcca gctcaccttt tcggggagta aatgccgctc tctccggcgc
     2641 atcgtctcac tcttctctat gatgaaatag ttaagcttcc ggtacatctc cggcaccgat
     2701 ttcagccggc taagaatgtc cctgctgagc actccggttc ccgcgccgat ttcaacgatg
     2761 gtgaagggga cgccgccgag caaccgccac atctcctcga actgggcgcc cagcaggtcg
     2821 ccaaataaac cggtcaggca ggggctcgtg aagaagtccc cgcgcttgcc gatctgctcg
     2881 cgcaccgacg tataataacc taaccgcgga tgatacaaag caagctccat gaactccctg
     2941 aagggcatcg gacccccggt tttaatttta tcgataatga tcgccgccag atgttgagtc
     3001 tcctgcatgt cgaaagatat aagtcaatca gtcggttgga atgtcacaag caccgtgccg
     3061 ggcgcccaaa tggcatggtt ttttcggcga actgggcatg tcaagcacta catctcttcc
     3121 ccagcaggac gcccccggcg cgagccgtgg cgatctcgag cagctgtgta tcaataccat
     3181 aagggtcttg tccgccgacg ccgtccaaaa agccaactcg ggccatccgg ggacgccgat
     3241 gggtacggct ccgatgggtc acgttctatg gtccgaggtc atgcgctaca acccgcgcga
     3301 tcccgcatgg ccaaaccgcg accgtttcgt gctttcagca ggccatgcgt gcatgctcca
     3361 atactgctat ctttttctta ccggttatga cctgtctctc gatgatatcc ggcatttcag
     3421 gcaattgcac agcaaaacgc cgggtcaccc cgaatacggc ctgacacccg ggattgaagt
     3481 caccaccggt cccctggggc agggctttgc caacagcgtg ggaatggcca tagcgcaacg
     3541 gcacgtcgcg gcacggttca accggccggg gtttgacctg ttcaactacc ggatctacgg
     3601 gatctgcagc gacggcgata tgatggaggg cgtcgcctcc gaagccgcgt cgatggccgg
     3661 acatctcggg ctgggccaga tcatcttcct ttatgatgac aaccatatct ccatcgaggg
     3721 aaacaccaat gtgactttct ccgaggacgt aggcaaacgg tttgaggcat acggctggca
     3781 cgttcaggta ctggaagacg gcaacgacac cggggccatc cgccaggcca ttcaacgggc
     3841 gcaggcggag actacaaggc cctccctcat caaggtcagg acgcatatcg gctacggcag
     3901 tccgaataag gtggatactg gcagcgcaca cggatcaccg ttgggcgccg acgaggtgaa
     3961 gctcgtcaag aagggttttg gtttcgaccc cgatgcgtcc ttcgtcgtac cggaacaagt
     4021 cctcgcattt taccggaagg cgggtgaaaa agggaccgct gcggaaaagc aatggaccgc
     4081 gctgtgggaa tcctacaggc aacagtttcc ggatctggca gccgagtggg aagcaatgcg
     4141 gcaggaacaa ctgccggggg actgggagaa gaaagtcccc gtcttccgcc agtcggacgg
     4201 caaggacggc cagatctcga cgcgggtcgc cgcgggcaag gtactgaaca gtatcgcacc
     4261 gctgttcccc cacctgatcg gcggctccgc cgatctcgcc ccttcgaccg aaacgaacct
     4321 caagaacttt tcttcttttt ccgcggacga ccccggcggc cggaattttc att
//
LOCUS       DATGKE010000133         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1517670, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000133 DATGKE010000000
VERSION     DATGKE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517670"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VKU83_02355"
     CDS             <1..1971
                     /locus_tag="VKU83_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HLI92418.1"
                     /translation="EGRFGNRLENMVDWNLSRSRYWGTPLPVWRTDDASEEICIGSLA
                     ELRDGILKSVAAGFMKPASGEEAEAMAERLTRDLHKPHVDEITLVSAKGLPMKRVPDL
                     IDVWFDSGAMPYAQWHFPFENKETFARNYPADFIAEGVDQTRGWFYTLHALGALIKES
                     VHEELAKAGLAGLAGGDLETRYPGLAYRTVVSNGLVLDKNGNKMSKRLGNVVDPFQTI
                     DKYGADATRWYLITNASPWESMKFDLEGIREAQRKFFGTLYNTYQFFALYANVDGFAF
                     KEEYIPAADRPEIDRWILSSLNTLITKVTAYFDDYEPTIAGRLIEDFLDEHLSNWYVR
                     LCRRRFWKGEYGFDKICAYQTLYECLETTSRLMAPISPFFSDWLFLNLNEITGRIRVA
                     SVHHADFPGSNPGQIDLLLEERMQMAQDASSLILSLRKKVNVKVRQPLQRVLIPVLEA
                     RMQEQLSQVEDLIKAEVNVKQIDYLAGNETFIKKKIKPNFVALGKRLGPRMKAVTAAL
                     SRFTQEDIAKLEKDGRITLFIDKEPLILATNDVDITSEDIPGWVVASKNALTVALDVT
                     VTPELAREGNAREFVNRIQKIRKDKGYEVTDRILVKVTDVPALKDSLTVFKDYICTEI
                     LADSIELVNEPANGTEIEVNDILLKVFVSKKA"
     gene            1976..2935
                     /locus_tag="VKU83_02360"
     CDS             1976..2935
                     /locus_tag="VKU83_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HLI92419.1"
                     /translation="MATKKKAAKPAKKTVNVKKIAPAKKAAAKPVHSSGSSKNAKKAP
                     AAKSIAKPVAKKVATAQAKKSAGPATTKKTAASHVARPAASQAKPAAGKGQLAGKPTS
                     VPSVAKAQPAPKTPVTAHAAAAKQAAPASAGKPAAAPTLPPTPAGTKPEPKKPAAPPK
                     QVKVVIPEIKTKTVRKYEPEFTKSVLDTPQEEQTGPIMRYSDAELGEFRELINRKLDA
                     AKKELAYLQGLITRKDEMGGDNDDARYMTMEDGSMSMEREQLSQMASRQITFIDHLEK
                     ALMRIENKTYGICRVTGKLIDKARLRAVPHATLSIEAKQMMNK"
     gene            3067..>3789
                     /locus_tag="VKU83_02365"
     CDS             3067..>3789
                     /locus_tag="VKU83_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92420.1"
                     /translation="MRRIRFAWLLVAFFGAQAQQTPVTKANYQLAARFSPKKLEKLIF
                     STSVDPHWLKKSDRFWYVYETTDGKKWYIVDPAKAEKRPLFDNDKMAAAISRVVKDPF
                     DAQHLGLDSLKFIRDENWIQFEVKSSEDIERKDTVKKKAGAADRPEKKVYYFEYNLLD
                     GTLTELKDYKKPKHKPAWANISPDSNTIVFGRHFNLYWMDRANYDKALINENDSTIVE
                     HQLTTDGIEDYSYHGNGLAGSVG"
BASE COUNT         1028 a          986 c         1022 g          753 t
ORIGIN      
        1 gaggggcggt tcggcaatag gcttgaaaat atggtagact ggaatctgag ccggagccgg
       61 tactggggca ctccccttcc tgtatggaga acggatgacg catcggaaga gatctgcatc
      121 ggttccctgg ccgagttgcg ggatggcatc ctgaaatccg tagccgcagg tttcatgaag
      181 ccagcttcag gtgaagaggc ggaagcaatg gcggaaaggc tgacccgcga tctccacaag
      241 cctcatgtcg acgagatcac cctggtcagc gcgaaagggc tgccgatgaa gcgggttccg
      301 gacctgatcg atgtctggtt cgatagcggc gccatgcctt atgcccagtg gcattttccg
      361 tttgaaaaca aagagacgtt cgcccggaac tatccggcgg actttatcgc cgaaggcgtc
      421 gatcagaccc gtgggtggtt ctacaccctg catgccctcg gcgcattgat aaaagagtct
      481 gtccatgaag aactggccaa agccggcctc gccggcctcg ccggcggcga cctggagacc
      541 cgttatccag gtttggctta ccgtaccgtg gtgagcaacg gcctggtcct cgacaagaac
      601 ggcaataaga tgtcgaagcg gcttggcaat gtcgtcgacc cgttccaaac catcgataag
      661 tacggcgccg atgcgacccg ctggtacctg atcaccaacg cttcgccctg ggaaagcatg
      721 aaattcgacc tggagggaat ccgcgaggcg cagcgcaagt tctttggcac gctatataat
      781 acctaccaat tttttgcatt atatgcgaac gtggatggct ttgcgttcaa ggaagaatat
      841 attccggcag cggatcgtcc tgaaatcgac cgatggatcc tctcttcgct gaacacgttg
      901 ataaccaagg ttacagccta ttttgacgac tacgaaccaa ccatcgccgg ccggctgatc
      961 gaagatttcc tggacgagca cctcagcaac tggtacgtgc gcctttgccg tcgccggttc
     1021 tggaaaggag agtacggatt cgacaagatc tgcgcctatc agacacttta tgagtgcctg
     1081 gagacgacca gccgcctgat ggcgcctatt tcgccgttct tttcagactg gctttttctg
     1141 aatctcaatg agataacggg gcggatcagg gtggcctccg ttcaccacgc cgattttccg
     1201 gggtccaacc ccggccagat cgacctcctg ctcgaagaaa gaatgcaaat ggcccaggat
     1261 gcctcttcgc tgatcctctc cttgcggaag aaagtgaatg taaaggtgcg ccagccgctg
     1321 caaagggtac tcatccctgt gctggaagcg cggatgcaag aacagctttc gcaggtggag
     1381 gacctcatca aggccgaagt aaacgtcaaa caaatcgatt acctggctgg gaacgagacg
     1441 tttatcaaga aaaagatcaa gccgaatttc gttgcgctgg gcaagcggct tggaccgaga
     1501 atgaaggctg taaccgccgc cctgagccgg ttcacgcagg aagacatcgc gaaattggaa
     1561 aaagatggcc gcattacttt atttatcgat aaagaaccct taatattggc gactaatgac
     1621 gtggatatca cgagcgaaga tatcccgggg tgggttgtgg caagcaagaa cgcactgacg
     1681 gtcgccctcg acgtcaccgt cacgccggaa ctggcccggg agggcaatgc ccgcgaattc
     1741 gtgaacagaa tccaaaagat ccgtaaagac aaaggctacg aagttacgga ccgcatcctg
     1801 gtaaaagtta cagacgtgcc cgccctgaag gactccttaa cggtttttaa ggattatatt
     1861 tgtacggaaa ttttggcaga ttccatcgaa ttggtcaatg aaccagccaa tggcactgaa
     1921 attgaagtga atgatattct tttaaaagtg tttgtttcaa aaaaagcctg actgtatggc
     1981 aaccaaaaag aaagcggcca aacccgctaa gaagaccgta aatgtcaaga aaatagcgcc
     2041 ggctaaaaaa gcggctgcga agcctgtcca ctcgtcaggc agcagcaaga acgcgaagaa
     2101 agcgccggcg gctaagagta ttgctaaacc ggttgctaaa aaggttgcaa cagcccaggc
     2161 aaaaaaatct gccggtccgg ccactaccaa gaaaactgct gcgagtcatg ttgccaggcc
     2221 ggccgcttcc caggcaaagc cggcggccgg gaaaggtcaa ctggccggaa aacccacgtc
     2281 cgttccttcc gttgccaagg ctcagcccgc ccctaagacc ccggtgaccg cgcatgcagc
     2341 tgccgccaag caggctgcac ccgcatccgc cgggaaacct gctgccgccc caacgctgcc
     2401 gccgaccccg gccggcacga aaccggaacc gaagaaaccc gcggcgcctc ccaaacaagt
     2461 aaaggtggtc atcccggaaa taaagacaaa aacggttcga aagtatgaac cggaattcac
     2521 taaatcagta ttagacactc cacaagagga gcaaactgga cctattatga gatacagcga
     2581 tgcagaactt ggagaattca gggaactgat caaccgcaaa ctggatgcag ctaaaaagga
     2641 attagcttac ctgcagggtt tgatcacgcg taaagatgag atgggcggtg ataatgatga
     2701 cgcccggtac atgaccatgg aggatggaag catgagcatg gagcgcgaac agcttagcca
     2761 aatggccagc cggcagatca cctttatcga tcatctggag aaagccctga tgaggatcga
     2821 aaacaaaacg tatggaattt gccgggtcac cggtaaattg atcgataagg caagattgag
     2881 agccgtgccg cacgctaccc tgagcatcga ggcaaagcaa atgatgaata agtaggaatc
     2941 cgatcctttt ttccggcata acccggctgc ggaaggtccg tctatccgtt aatgccgccg
     3001 cgcgtggata catgagacac ggattccgga agaaagagta tatagcagta taaattccaa
     3061 ctgcttatgc gccgcatccg ctttgcgtgg ctactggtgg ctttttttgg agcacaagcc
     3121 cagcaaaccc ccgttacaaa ggccaactat caactggctg cccgtttttc acccaagaag
     3181 cttgaaaagt tgatcttcag cacttctgtt gatccccatt ggctgaagaa gagcgatcgt
     3241 ttctggtatg tgtacgaaac caccgacgga aagaaatggt atatcgtgga cccggcgaaa
     3301 gcagagaagc ggcctctttt tgataatgat aaaatggctg cggcgatctc tcgtgtcgta
     3361 aaggacccat tcgatgccca acatcttggc ctggacagcc tgaaattcat tcgcgatgag
     3421 aattggatac aatttgaggt gaagagctcg gaggacatcg agcggaagga caccgtaaaa
     3481 aagaaggccg gcgcggctga ccggccggaa aaaaaggttt actatttcga atacaatctc
     3541 ctcgatggaa ccctaaccga actgaaagac tacaaaaagc ccaaacataa acccgcgtgg
     3601 gccaatatct cccccgacag caacacgatc gttttcggcc gccatttcaa cctgtattgg
     3661 atggaccggg ccaattacga taaggcgctg atcaacgaga atgacagtac catcgtcgag
     3721 catcaactga ctaccgatgg catcgaagac tacagctatc acgggaatgg tttggccggc
     3781 agtgtgggc
//
LOCUS       DATGKE010000134         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_69931, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000134 DATGKE010000000
VERSION     DATGKE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69931"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1203
                     /locus_tag="VKU83_02370"
     CDS             88..1203
                     /locus_tag="VKU83_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HLI92421.1"
                     /translation="MAKLSGRETAFLRMSNQLICGSRFDDAGEMVRRMACVQAQDYSM
                     AKWALGCRLASATDCSVEAAFQESSFLRTHVLRPTWHFVHPADIRWMLALSAPKIKAL
                     SAPYLRKLGIDQAVLRKSRKLIVRSLVETPRLTREELTAVLIRGKVAVNDGRISFLLM
                     DAELDAIICSAGRERKKFAYQLLDNVVAAGALPGREQSIAELARRYFTSRGPATLADF
                     AWWAGLTVAEARVGVELNEKSLEQAEANGKVYLFAAEMEERQTGERSKILAEGTFLLP
                     AFDEYTVGYKDRSLVLDPRFAAQCNSGLSPVVVHKGQIIGTWSRAPGKGAGTKIREGK
                     NPAVQEHGEVKIRLFRGVRPSGALRAALKKYSAFKSG"
     gene            complement(1233..>2294)
                     /locus_tag="VKU83_02375"
     CDS             complement(1233..>2294)
                     /locus_tag="VKU83_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLI92422.1"
                     /translation="FAGGVFLLSSAKRNEMTKISSRRSFLRNTTLGAVGMGLGAALPA
                     RAFASAPAPVNPDDLFFKIAVSQFSFASQFWTGKLDPLDFPAKSKELGITGLDYCSMF
                     FATKAKDAGFLADLKKRSADAGTYNLRIMVDGEGVLGDLNDANRLKAVDNHYKWIDAA
                     SALGCPMIRVNVEGEGAPEDVAKAAVDSLGRLIEYGRRTDTDVIVENHIGISCNAGWL
                     AGVMKQLNSPHGGTLADFGNFCINRTKPETMDIPGYLKTKCLEEYDRYKGITELMPYA
                     KGVHAKSHLFNPDGNDQETDFNRMFRIIKDSGFTGWVSVEYEGGLLKMYTKDEQYLDD
                     YAGTAATKKLVEKAGSLVS"
BASE COUNT          480 a          592 c          685 g          537 t
ORIGIN      
        1 tttatttacg agacaagcca gcgactggaa catcgaccgg tgtaccgctc ctaaaccatt
       61 tggaaagatc gctgtaactt gggcccgatg gccaaactgt cgggcaggga aacggctttt
      121 ctccggatgt cgaaccagtt gatctgcggt tcgcggttcg atgacgcggg cgaaatggtt
      181 cgccggatgg cctgtgttca ggcccaggac tattccatgg cgaaatgggc gcttggctgc
      241 cggctggctt cggctacgga ttgctccgtg gaagcggctt tccaggagag tagttttctc
      301 cggacacatg tcctgcggcc tacctggcat tttgtgcacc cggcggacat ccggtggatg
      361 ctcgccctga gcgcaccaaa aattaaggct ttgagcgccc cgtatcttcg aaagttgggg
      421 atagatcagg ccgtcctgcg caagagcagg aagttgatcg tccgttcgct tgttgagacg
      481 ccgcgtttga cacgtgaaga actgacggcg gtccttattc gcgggaaggt tgccgttaat
      541 gatggccgga tcagtttttt gctgatggac gcggaattag atgcgatcat ctgcagcgcc
      601 ggccgggaga ggaagaaatt tgcctaccaa ttactggata atgtggttgc cgccggagcc
      661 ttgcctggcc gtgagcaatc gatcgctgag ctggccaggc ggtattttac cagccgcggc
      721 cccgcgacgc ttgcagattt tgcctggtgg gcagggttga cagtggctga ggcaagagta
      781 ggagtggagt tgaatgaaaa atcgttggaa caagcggagg ccaatggtaa agtatattta
      841 tttgccgctg agatggaaga acgccagacg ggtgagcgaa gtaaaatcct cgcggaggga
      901 acctttttat tgccggcttt cgacgaatac actgtgggat ataaggatcg gagtttggtg
      961 cttgatccgc gatttgccgc ccaatgcaat tccgggttga gcccggtggt cgtgcacaag
     1021 gggcagatca tcggtacctg gagccgtgcg ccggggaagg gcgccggcac taagatccgc
     1081 gagggaaaga acccggcggt gcaggaacat ggcgaggtga aaatccgact attcagggga
     1141 gttaggcctt ccggcgcact gcgcgctgcg ctcaaaaagt attcggcgtt taaatcgggg
     1201 taggtcgacc ggccggggcc ataaactttc cattatgaga ccaggctgcc cgctttctcc
     1261 accagcttct tggttgcggc ggtgccggca taatcgtcga gatattgctc gtcctttgtg
     1321 tacattttga gcaggccgcc ttcgtattcc acgctgaccc agccggtaaa gccggagtcc
     1381 ttgatgatgc ggaacatgcg gttgaaatcg gtttcctggt cgttcccgtc cggattgaac
     1441 aggtgagatt tggcatgaac accctttgcg tacggcatca gctcggtgat acccttgtag
     1501 cggtcgtatt cctccaggca ctttgttttc aggtaacccg ggatatccat ggtttccggt
     1561 ttcgtgcggt taatgcagaa atttccaaag tcggcgaggg tgccgccatg cgggctattc
     1621 agttgtttca tgacgcccgc gagccagccg gcgttgcagg agatgccgat atggttttcc
     1681 acgatcacgt cggtatccgt cctgcgtccg tattcgatca ggcggccgag actatctacg
     1741 gcggctttcg ccacgtcctc cggtgctcct tctccttcta catttacccg gatcatcgga
     1801 cagccgaggg cggatgccgc gtcgatccat ttataatgat tatcgactgc ctttaaccgg
     1861 ttggcgtcgt taagatcgcc gagtacccct tcgccatcga ccatgatgcg caggttatat
     1921 gttccggcgt cggcagagcg ttttttgaga tctgccagaa agcctgcatc tttcgctttg
     1981 gtggcaaaaa acatggaaca atagtccaac cccgttattc ccaactcttt tgattttgcc
     2041 gggaagtcca acggatccag cttccccgtc caaaactggc tggcgaacga aaactgggag
     2101 accgcaatct taaagaaaag atcgtccgga tttaccggtg ccggcgcaga agcaaatgcg
     2161 cgcgccggaa gagcggcgcc caatcccatg cctactgcgc caagggtcgt attccggaga
     2221 aagcttcggc gggaactgat cttcgtcatc tcgttacgtt tagccgaaga taggagaaaa
     2281 acgccgcctg cgaa
//
LOCUS       DATGKE010000135         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_899621, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000135 DATGKE010000000
VERSION     DATGKE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899621"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VKU83_02380"
     CDS             complement(<1..1496)
                     /locus_tag="VKU83_02380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLI92423.1"
                     /translation="MRDRIVRPGLILFALSLAPHVKAQRVAAFVAGLDSIKAGLRIPA
                     MEAGVMQAGSVLLEKGFGVPSTAVFRVASITKTFTSTLIMQLVGKGRLNLDSGIIRYG
                     VDLGNPDITVRELLTHTSEEKPGSWFSYNGYRYGRLGAIIARVAGKPYYQLLMENIVK
                     PLDLYSTAPGDSLTHYLAYIEQHRDMWDFFETAHARLVRPYVLDSTGRLREAAYLDEF
                     GAFGGLATTVGDLMKYSAAIDRNALVSPATQQQIFTANRTADGKLTPYGLGWFVQNYR
                     GTEFYWHYGQTSPGESGIFIKVPARKLTVAVLANTDKLSAPFPLGDGDLFMSPVGQLV
                     YRCFIADGPADKDFQNKELIVRASMDEVHGDTAGAQRLYEAYGRENFTGRPPSPPAGK
                     EVVGFRNVRTSQDLSKPFRLKRRTSLRVYGVGEDCSGDHRTWCDFGWIEDAQGNIVWQ
                     MPGQPVEPAGGAAKNQRVDAVVNLPRGRYTLRYKSDGGHAYGSWDSLPP"
     gene            1552..2403
                     /locus_tag="VKU83_02385"
     CDS             1552..2403
                     /locus_tag="VKU83_02385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI92424.1"
                     /translation="MVAFLGMGLLGSNFVRAMINKGEKVRVWNRTPGKAKALEQYGAT
                     AHLTPAEAVQGADRVHLTIKDDASVDEVLAMASPGLQAGATIIDHTTTSVEGAISRTR
                     DWKARGFTYLHAPVFMGPSNALEGTGFMLVSGDREVVKRCTPVLEKMTGKLLDLGAET
                     GRAAAMKLLGNCFLVAFTTGIADTLMLAKAMQVPVEDLMHLFDNWNPAAMLTGRLKRM
                     TSGNFTNPSWELSMARKDTGLFLEEAKRGGAKLAVIPAIAAEMDRWIQKGHGGDDWTV
                     IAKDASR"
     gene            complement(2400..2804)
                     /locus_tag="VKU83_02390"
     CDS             complement(2400..2804)
                     /locus_tag="VKU83_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLI92425.1"
                     /translation="MKMITVVVLLAAVVTIGAAGATRFAKGGGFRNLKVLPKDISSKD
                     LQGIMADDFEDGLGVSCGFCHAGAPGGHGLDFASDAKPEKSIARAMMRLTLALNKRYF
                     RVRKPMIGSEALAVTCSTCHRGQPFPDDGGLP"
     gene            complement(2810..>3186)
                     /locus_tag="VKU83_02395"
     CDS             complement(2810..>3186)
                     /locus_tag="VKU83_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLI92426.1"
                     /translation="KGLSASTPVVVFAHIPLWAIYPQWGWGTQDGEQVLSMLRRFGSV
                     SVLNGHIHQTIQKVEGNMTFHTACSTAFPQPAPGSAPSPGPMKVPAEKLRSVLGLTEV
                     KYVGHDHTLAVTDTPLTEGAGS"
BASE COUNT          651 a         1008 c          920 g          607 t
ORIGIN      
        1 ggcggcagcg aatcccagct gccataggca tggcccccat ccgacttgta ccggagcgtg
       61 tatcggcccc ggggcaggtt cacgacggcg tcgacccgct gattcttggc cgccccgccg
      121 gcgggctcga ccggttggcc cggcatctgc cagacgatgt ttccctgcgc atcttcgatc
      181 cagccaaagt cacaccaggt gcgatgatcg cccgaacaat cctccccgac tccgtacacc
      241 cgtaagctgg tccgccgctt cagccggaaa ggcttactga ggtcctgact ggtcctgacg
      301 ttgcggaatc ccaccacttc ctttcccgcc ggcggcgacg gcggccgccc ggtgaaattc
      361 tcccggccgt acgcttcata caagcgctgc gcacccgcgg tgtcgccatg cacctcgtcc
      421 atggaggccc gcacgatcag ctctttgttc tgaaaatcct tgtcggccgg cccgtccgca
      481 atgaaacacc ggtacaccag ttgccccacc ggcgacataa agaggtcgcc atcgcccagc
      541 gggaatgggg cactgagctt gtccgtattt gccagcaccg ctacggtcag ctttctcgcc
      601 ggaaccttga taaaaattcc cgattcgccg ggcgaggttt gcccataatg ccaataaaac
      661 tcggtccccc ggtagttttg tacaaaccac cccaggccgt acggggtcag tttcccatcg
      721 gcagtccggt tggcggtaaa gatctgctgc tgggttgccg gggataccaa tgcattccgg
      781 tcaatggcag ccgagtattt catgaggtcc cccacggtgg tcgccaggcc gccgaaggcc
      841 ccgaactcgt ccagataggc agcctcccgc agccggccgg tactgtccag cacataaggc
      901 cggacaagcc gggcatgagc ggtctcgaaa aagtcccaca tgtcccggtg ctgttcgata
      961 taggcgagat aatgggtcag cgaatccccg ggcgccgtgg aatacagatc cagcggcttt
     1021 acgatatttt ccatcagcag ctgatagtag ggcttccccg ccaccctcgc gatgatggcg
     1081 cctaaccgcc cataacggta accattatag gagaaccaac tgcccggctt ttcttccgaa
     1141 gtatgcgtca gcaactcccg taccgtaata tccgggttgc cgaggtccac cccgtaccgg
     1201 ataatcccgg agtcgaggtt gagcctgcct ttcccgacga gttgcatgat cagcgtcgaa
     1261 gtaaaagttt ttgtaatgga tgccacgcgg aaaaccgcgg tggaggggac cccgaatcct
     1321 ttctcgagca ggacggaccc ggcttgcatc acgccggctt ccatcgccgg gatccgcaat
     1381 cccgccttga tcgagtcaag gcccgccacg aatgcagcca cccgctgggc tttcacgtgg
     1441 ggggcaagcg agagggcgaa aaggatcagc cccggtcgga cgatacgatc ccgcatgcgc
     1501 ttttgggcgc aaattacatg tttgattacc taactttata aaaaaaacat catggtagca
     1561 ttcttaggca tgggtttgct gggctcgaac ttcgtccggg cgatgatcaa taagggtgaa
     1621 aaggtccgcg tgtggaaccg caccccggga aaggccaaag cgttggaaca atatggtgcg
     1681 acggcccacc tcacccccgc cgaggcggtg cagggcgctg acagggttca tctgacgata
     1741 aaggacgatg cgtccgtcga cgaggtgctc gccatggcaa gccccggcct tcaggcaggc
     1801 gcgaccataa tcgatcatac caccacatcc gtcgagggcg cgatcagccg gacccgggat
     1861 tggaaagcgc ggggctttac ctacctgcac gcccccgtgt tcatgggacc ttcgaatgcc
     1921 ctcgagggta cgggcttcat gctggtatcg ggcgatcggg aagtggtcaa acgctgcacg
     1981 ccggtcctgg aaaaaatgac cggcaagctg ctggatctgg gcgcggagac aggccgcgcg
     2041 gcggcaatga aattgctggg aaattgtttt ctcgtcgctt tcactaccgg catcgccgac
     2101 accctgatgc tcgcaaaagc catgcaggta cccgtcgaag acctgatgca cttgtttgac
     2161 aactggaacc cggccgcgat gctcaccggc cggctgaagc gaatgacttc cggaaatttc
     2221 accaacccat cgtgggaatt gagcatggcc aggaaggaca ccggcctttt cctcgaggaa
     2281 gccaaacgcg gcggcgccaa actggcggtc atcccagcca tcgccgcgga aatggaccgt
     2341 tggatccaaa aaggacatgg cggcgacgat tggaccgtga tcgccaaaga cgcttcccgt
     2401 tagggtaatc ccccgtcgtc ggggaacggc tgcccccggt gacaagtgga gcaggtgacc
     2461 gccagcgcct cgcttccgat catcggtttc ctcacccgga agtatcgttt attgagcgcg
     2521 agagtcagcc gcatcatcgc gcgggcaatc gacttctccg gcttggcgtc gctcgcaaag
     2581 tcaaggccgt gcccgcctgg cgcgccggca tggcaaaacc cgcagcttac tccaagcccg
     2641 tcttcgaaat cgtccgccat gataccctgc aggtcttttg acgaaatatc ctttggcagg
     2701 actttcaggt tccggaagcc gccgcctttg gcgaaccgcg tggcgcccgc cgccccgatg
     2761 gtcacgacgg cagccagcaa tacaacgacc gttatcattt tcatggggat catgatccgg
     2821 cgccctccgt cagcggggtg tcggtcacgg caagcgtatg atcatgcccg acatatttga
     2881 cttccgtcag accgagaacg cttctcagtt tctccgccgg caccttcatc ggcccaggcg
     2941 acggcgcact gcccggcgca ggctgtggga aagccgtgct gcaggcggta tggaaggtca
     3001 tattcccttc cactttttgt atggtctggt ggatatggcc gttcagcacc gagacggaac
     3061 cgaaacgcct cagcatcgac agcacctgct cgccgtcctg cgttccccag ccccattgcg
     3121 ggtagatggc ccaaagcggg atatgcgcga agacaaccac gggcgtgctg gcggaaagcc
     3181 ctttca
//
LOCUS       DATGKE010000136         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1313833, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000136 DATGKE010000000
VERSION     DATGKE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313833"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="VKU83_02400"
     CDS             <1..2200
                     /locus_tag="VKU83_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92427.1"
                     /translation="YERDYFNGNVLAGYPTQGLLAEELKRVMPEVQYAAGLESVAPPG
                     AANTLALGEKITKMNGFFAGADFFRMFSFPILQGQAATALNKPGTIAISRKVATYFFG
                     SPQEAVGKALTFDNREALLVSAVFGDVPANSSLQFDFLRSWTDFVKQNDWVNNWGNTD
                     PETFVQLRPGTDPAHLETLIRDFIYRYRPRTDGARTELALQPFPDKYLHSAFRDGRIA
                     GGRIEYVRLFTLVAIFILLIACINFMNLATAQSGRRAKEVGLRKVIGAVRSALIGQFI
                     GEAMLLTLLSVCLALLLTDAALPAFNSLTGKQLLLPVTQPFFWILVLSLLLVTGLVAG
                     SYPAFFLSGLQPVKVLKGSLKFTGSSLFLRKGLVVFQFSLSVILIVSTIVIYRQTTYI
                     RSTNIGYDRENLVYIPIEGDLVKNYTVFKQKAGDLAAVLSVSKMRNSPSDIQHHTGDI
                     FWPGKDPNAHIRFADGVVGYDFVKTMRLQLKRGRDFSKDYPSDSVGFLLNETAVRVMG
                     LRNPLGSTVTWGRHPGKVVGVIKDFHFNSLHQTIDPLIIRLDEDWSWGTILVRTRAGM
                     TRQAVSGLENLCRELNPKFPFTYQFSDEAFNRQYRSDEVVSQLSEVFAFLAIFISCLG
                     LLGLAAFTAAQRTREIGVRKVLGATAASIATMLSRDFLVLVLLAFLIASPVAWLAMRQ
                     WLGSFAYRIDVGWWTFGLAAVTVVTIAFLTVAWQTLQAALANPVNSLRME"
     gene            complement(2204..>3053)
                     /locus_tag="VKU83_02405"
     CDS             complement(2204..>3053)
                     /locus_tag="VKU83_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI92428.1"
                     /translation="ILTYSTGARFDSIVKKYERYKARFAIWCGFDYTGYERPGWQAHA
                     IAELERCHREGAVGVGELGDKGLGEYYSKPVPGYGMHIDDPRMKPLLAKCGELHMPVS
                     IHVAEDAWMYLASDSTNDGLMNAATWKVDMTVKGKLGHDQLIATLANAVRENPGTTFI
                     ACHLANCCSDLSVLGRMLDKYSNLWADIAARYGEISPVPRYTRAFMEKYADRLVYGTD
                     MGFSVRMYRTTFRILETRDEHFYAQWLLNYHWPLYGLGLPDATLKKLYHDNAARLFSQ
                     AAGSGR"
BASE COUNT          628 a          901 c          824 g          700 t
ORIGIN      
        1 ttatgagcgg gattatttca acggcaatgt cctcgccggg tacccgacgc agggactcct
       61 cgcagaagaa ctgaaaagag tcatgcccga agtccagtac gcggcgggct tggagtctgt
      121 tgcacccccg ggtgctgcaa acactctggc gttaggagag aagatcacca agatgaatgg
      181 gttctttgcc ggcgccgact ttttccggat gttcagcttt cccatccttc agggacaagc
      241 cgccacagcc ctgaacaagc cgggcaccat tgctatttcc cgcaaagtcg cgacgtattt
      301 ttttggaagc ccgcaagaag ccgtcggtaa agccctgacc tttgataacc gggaggccct
      361 gctggtttct gcagtattcg gggatgtccc cgccaattcc tcgctgcagt ttgattttct
      421 ccggtcctgg acagactttg tgaagcagaa cgactgggtg aataattggg gcaacaccga
      481 cccggagacc tttgtgcaac tccggcccgg aacggacccc gcccatttgg agaccctgat
      541 cagggacttt atctatcgtt atagaccccg cacggacggc gcacgcacgg agctcgctct
      601 gcagccgttc cctgacaaat accttcactc agcattcagg gacggccgga tcgcaggggg
      661 ccgtatcgaa tatgtgcgcc tcttcacgct ggtggcgatc tttatcctgc tgatagcctg
      721 catcaacttt atgaacctgg ccactgccca atcgggacga cgtgcaaagg aagtcggcct
      781 tcgcaaagtc atcggcgcag tgcggtcagc attgatcggt cagttcattg gcgaggccat
      841 gctgcttacg ctcctgtcgg tctgtctggc cttgctgctg accgacgccg cgttgccggc
      901 tttcaacagc ctgaccggaa aacagctgct cctgcccgta acccaaccct tcttttggat
      961 cctcgttctc agcctgctgc ttgtcaccgg cctggtagcg ggaagctatc ccgccttctt
     1021 cctttccggg ctgcaacccg tgaaggtatt gaagggcagc ctaaaattca cgggatcttc
     1081 gctatttctg cgaaaaggcc ttgtcgtgtt ccagttctcg ctgtcggtga tactgatcgt
     1141 ctcgacgatc gtgatctacc ggcagacaac ctatattcga agcacgaaca tcggctatga
     1201 tcgcgaaaac cttgtttata tccctatcga aggggacctg gtgaagaact acaccgtgtt
     1261 caagcaaaag gccggagacc tggcagctgt cctcagcgta tccaagatgc ggaattcccc
     1321 ttcggatatc caacaccata cgggcgacat tttttggccc ggcaaagacc ccaacgcgca
     1381 cattcggttc gccgacgggg tagtgggata cgacttcgtg aagaccatga ggctgcaatt
     1441 gaaacgaggc cgggatttct caaaggatta cccgtccgac tccgtcgggt tcctgctgaa
     1501 tgaaacggcg gtcagggtca tgggtctccg gaacccactg gggtcgaccg tgacatgggg
     1561 ccgtcacccg ggcaaagtag tcggagtgat caaggacttt cattttaatt ctctccacca
     1621 gaccatcgat ccccttatca ttcgcctgga tgaggactgg tcatggggca cgatcctcgt
     1681 gcggaccagg gccggcatga cgcgacaggc agtctcggga ctcgaaaatt tgtgcaggga
     1741 gctgaatccg aaattcccgt tcacctatca attctcagac gaagcgttta accgccagta
     1801 ccggagtgac gaagtggtca gccagctctc tgaagtcttt gctttcctgg ccattttcat
     1861 ctcctgtctc gggctgctcg gcctggccgc ctttacggca gcgcagcgca cccgcgagat
     1921 tggcgttcga aaggtcctgg gagctacagc agcgtccatc gctaccatgc tctcgcggga
     1981 tttcctggtg ctggtgctgc tcgccttcct tatcgcttct cctgtcgcct ggctggccat
     2041 gcgccagtgg ctcggaagct ttgcctaccg gatcgatgtc ggctggtgga ccttcggcct
     2101 ggcagccgtc acggtcgtga ccatcgcctt cctgacggtg gcctggcaaa cgctgcaggc
     2161 agccctcgcg aacccggtaa atagcctgag aatggaatag aacctatcga ccgcttccgg
     2221 ccgcctgact gaaaagcctc gcggcattgt cgtgataaag ctttttcagc gttgcgtccg
     2281 gcaagccgag cccatagagc ggccagtgat aattcaataa ccactgggca taaaaatgtt
     2341 cgtcgcgcgt ctcgaggatc cggaacgtcg tccgatacat cctgacggag aaacccatgt
     2401 ccgttccata gacgagcctg tccgcatatt tttccataaa agccctggtg taccttggaa
     2461 ccggggaaat ctcgccataa cgggctgcaa tatccgccca aaggttggag tatttgtcga
     2521 gcattcttcc gagcacactc agatcggaac agcagttggc gaggtggcag gcaatgaagg
     2581 tcgttccggg attctccctg accgcattcg cgagggtagc gatgagttgg tcgtgaccta
     2641 atttgccctt tactgtcatg tctactttcc acgtggcggc attcatcagg ccgtcattgg
     2701 tggaatcgga cgcaagatac atccacgcgt cttccgccac atggatgctg accggcatat
     2761 gcaattcacc gcatttggcc aggagcggct tcattcgcgg gtcgtcaata tgcatcccat
     2821 atcccgggac cggttttgaa taatattcgc ccaggccttt atcgcccagc tcaccgacac
     2881 ccaccgcgcc ttccctgtgg caccgctcca gctctgcaat agcatgggct tgccagccgg
     2941 gccgctcgta gcccgtgtag tcaaacccgc accagatcgc aaacctcgct ttgtatcgtt
     3001 cgtatttttt cacgatggaa tcaaaacggg cgcccgtcga gtaggtaagg atc
//
LOCUS       DATGKE010000137         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_594756, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000137 DATGKE010000000
VERSION     DATGKE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594756"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VKU83_02410"
     CDS             complement(<1..456)
                     /locus_tag="VKU83_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLI92429.1"
                     /translation="MTTNFDVIIIGGGPIGIACALEAQKAALHYLVLEKGCLVNSLYN
                     YPANMTFFSTSERIEIGNVPFVSNNVKPNRAEALEYYRRVAVSNHLNIRLQERVHEVR
                     PDGDGFTVISSQAVYRANNVVLATGFYDIPVLMGVPGEDLPKVTHYYKEP"
     gene            612..1559
                     /locus_tag="VKU83_02415"
     CDS             612..1559
                     /locus_tag="VKU83_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLI92430.1"
                     /translation="MDASSLVTIILLVLLAAILFSCWVTVNQGYIYVVTLFGKYRRIL
                     RPGLNFKMPFFEQVYSRISIQNRSVELEFQAVTADQANVYFKSMLLYAVQNADEETIK
                     KVAFKFISDRDLMQALTRTIEGSIRSFVATKRQAEILGLRKEIVEFVKDQIDHSLADW
                     GYHLMDLQINDITFDKAIIESMSKVVASNNLKAAAENEGQALLITKTKAAEAEGNAIK
                     ISAEAEKEAARLRGQGVALFRQEVAHGMSEAAEEMRKANLDTNVILFSMWTEAIKNFA
                     EVSKGNVIFLDGSSQGMENTMRQIQALMLRPVGTGGGTN"
     gene            1737..2441
                     /locus_tag="VKU83_02420"
     CDS             1737..2441
                     /locus_tag="VKU83_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLI92431.1"
                     /translation="MNLFLLQVIVDSAAIKDSLIRAAQLAAQQKESLIDILVKGGPLM
                     IPLAILLVIAIFVFFERYIAISRASKVEDNFMNIIRDNIMTGNVSAARNLAKNTPTAV
                     ARIIDKGIQRVGKEIADIERSMENIGKLELYKMEKNLAILSAIAYLGPLFGFLGTIFG
                     MFELFYAIASTGEYSISNIAAGIYVKMISSAAGLIIGIVAYVGYRFLNSQIDKATNKM
                     EAASADFVDILQEEVR"
     gene            2443..2850
                     /locus_tag="VKU83_02425"
     CDS             2443..2850
                     /locus_tag="VKU83_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLI92432.1"
                     /translation="MNLRKRHRADETEVHTGPLNDILFILLLFFLIVSTLANPNVVKL
                     SQPKSKSDTKSKQTVVVSIDANKQFYVGTTKVSLDQLKAQLQPFLAKETEQPSIVINA
                     DKTVPIDDVVAVMRVARELGARTVLAVDKNGVK"
     gene            2974..>3267
                     /locus_tag="VKU83_02430"
     CDS             2974..>3267
                     /locus_tag="VKU83_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92433.1"
                     /translation="MRKKLVFIIAVMSGMSAAEAQTSDSAGQSGWSNHFQLTVINQIH
                     SGFKAAYSGPNSLADSVEPAATSVTTTLFLGRRLWRNAAVYANPEVSGGRGLSY"
BASE COUNT          802 a          816 c          940 g          709 t
ORIGIN      
        1 cggctcctta tagtaatgcg tgacttttgg taaatcctcg cccggcactc ccatcaggac
       61 aggaatatca tagaagccgg tagccaggac cacattgttt gcgcggtaga ctgcctggct
      121 gctgatgacg gtaaagccgt cgccatccgg ccgcacctcg tgcactcttt cctgcagccg
      181 gatgttcagg tggttcgaaa ccgcgacgcg gcgatagtat tccagcgcct ccgcgcggtt
      241 gggtttgaca ttgttgctga caaagggcac attaccgatc tctatccttt cggacgtcga
      301 aaagaacgtc atgtttgcag gatagttata aagggagttg acaagacagc ctttttccag
      361 aaccaggtaa tggagtgctg ccttttgcgc ttccagcgca caggcgatgc cgatcggccc
      421 gccgccgatg ataataacgt cgaaattcgt ggtcattggg cagattgcgg tgcgaagtta
      481 ccatataata cgggtgagaa ggacggccgc cgaaaaatag catcattcca cgggccgccc
      541 ttcgctgcgt ggccctggcc gtcttgagcc aattcgataa aaagcgtaca ttcacccgga
      601 aaaatctttt tatggacgca agcagtttag ttaccattat ccttttggtc ctactggccg
      661 ccatcctgtt cagttgctgg gtgacggtga accagggata catttatgtt gtgacgctct
      721 tcggaaagta ccggcgcatc ctgcggcccg ggctgaattt caaaatgccc ttcttcgagc
      781 aggtctacag caggatctcg atccagaacc gttccgtgga actggagttc caggcggtca
      841 cggccgacca ggcaaatgtg tacttcaaaa gcatgttact atatgccgtg cagaacgcag
      901 acgaagagac catcaagaaa gtggcgttca agttcatcag cgaccgggat ctcatgcagg
      961 ctttgacgcg gacgatcgag ggaagcatcc ggtcatttgt cgccacgaaa aggcaagccg
     1021 agatcctggg attgagaaaa gagatcgtgg aattcgtgaa ggaccagatc gatcactccc
     1081 tggcggactg gggttaccat ttgatggatc tgcagatcaa tgatatcacc tttgacaagg
     1141 cgatcatcga atcgatgagc aaggttgtgg caagcaataa cctgaaggcc gcggcggaga
     1201 atgaaggaca ggccctgctg atcacgaaga cgaaggcggc ggaagccgag ggcaacgcga
     1261 tcaagatctc cgccgaagcg gaaaaggagg cggcgcggtt gcgcggccag ggggttgcct
     1321 tgttccgcca ggaagtggcg cacggcatga gcgaggcggc ggaggagatg agaaaggcga
     1381 acctggatac gaacgtcatc ctgttcagta tgtggacaga ggcgatcaag aattttgccg
     1441 aggtcagcaa gggaaatgtg atctttctgg atggcagcag ccagggcatg gagaatacga
     1501 tgcggcagat ccaggcgctg atgctgaggc cggtaggtac cggcggcggc acgaactaag
     1561 cgctggtcaa acgccattcc ggcgctgcgc ggataacttt agcattttgt taaaaaatag
     1621 acggggccgg agatcgttat agtcgtaaat ctttactttt accgatcgga aggcggctcg
     1681 ccaccggcgg caaagatacc gatcagccga tttttcacat aaaaccgttt attggaatga
     1741 atttatttct tctgcaggtc attgtagatt cagcagcgat caaagacagc ctgatcaggg
     1801 cagcacagtt ggcggcacag caaaaggaaa gtctcatcga tattcttgtt aagggcggcc
     1861 cgctgatgat ccccctggcg atcctgctgg tgatagcgat cttcgttttc ttcgagcggt
     1921 atattgccat ttcgagggct tcgaaagtag aggataattt catgaatatc atccgcgaca
     1981 acatcatgac aggtaatgtt tccgccgcca ggaacctggc aaagaacacc ccgacggcgg
     2041 ttgcacgcat tatcgacaag ggtatccagc gggtaggaaa ggagatcgcg gatatcgaga
     2101 ggagcatgga aaatatcggc aagctggaat tgtacaagat ggaaaagaac ctcgctatcc
     2161 tgtcggcgat cgcctacctc ggaccgctgt tcggattcct cgggacgata ttcgggatgt
     2221 tcgaattgtt ctatgccatt gcaagcacgg gagagtacag catttcaaac atcgcggccg
     2281 ggatctatgt aaagatgatc tcgtcggcgg ccgggttgat catcgggatc gtcgcttatg
     2341 tcggttaccg gttcctgaat tcccagatcg acaaggccac caacaagatg gaggcggcga
     2401 gcgcggattt cgtggacatt ctgcaggagg aggtgagata atatgaacct tagaaaacgg
     2461 catagggcgg atgaaacgga agtccacaca ggaccgttga acgatatttt gttcattctc
     2521 ctgttgttct tcctgatcgt gagcacactg gccaacccga acgtggtgaa gttgtcgcag
     2581 ccaaagtcga aaagcgacac aaagtccaag cagaccgttg tcgtgtcgat cgatgcgaac
     2641 aaacaattct atgtgggcac gacgaaagta tcgctggatc agctaaaggc ccagctgcaa
     2701 ccctttttgg caaaggagac cgaacagccg tccatcgtaa tcaatgcgga taagactgtc
     2761 ccgatcgacg atgtggttgc cgtgatgcgg gtcgcccgcg aactgggggc aaggaccgtc
     2821 ctggcggtgg acaagaatgg cgtcaaatag ccgcccggca ttccgccgcc acctggttgc
     2881 aatcatttca tttcgaactt ttctaaaatt tcctagtttc gcgccacccg ttggtgcgcc
     2941 tgaacggctg ccggccaatg gcattgaacg gctatgcgaa agaaactggt ttttattatt
     3001 gcggtcatgt cggggatgtc ggcagccgaa gcccaaacca gcgattcggc cgggcagagc
     3061 ggctggagca accattttca actgacggtc atcaatcaaa tccattccgg gttcaaagcc
     3121 gcctatagcg gaccaaacag cctggctgat tcggtggagc ccgccgcaac cagcgtgacc
     3181 accaccttgt tcctgggcag gaggctttgg cggaatgccg ctgtctatgc caatcccgaa
     3241 gtctccggcg gccgcgggct tagctat
//
LOCUS       DATGKE010000138         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_690457, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000138 DATGKE010000000
VERSION     DATGKE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690457"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VKU83_02435"
     CDS             <1..1457
                     /locus_tag="VKU83_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92434.1"
                     /translation="ARAATRLKEVGIKKVVGALRRDLALQYLAEAVMMAVFAFLVAIG
                     VVIALLPQFNILTGKHLALQFNPNVILSVIGILLFTGVAAGSYPAFYLSGFRPVAILK
                     NKLKSSIGEVIARKGLVIFQFTLSIILIVAVLVCNRQIEYIQTTNLGYSRDNVVLIKM
                     EGDISKNLESFLTELKSTPGVLNASAMSGNITESGNSTGPGGVTWRGRDPKDATSFEY
                     REVNNDFVETLGMSMAEGRSFSRAYPNDTSGIIFNETAIKAMGLKDPIGQAVTYNGTQ
                     MHIIGVVKDFHFQSFHEAIKPLLLVAVPAENDRIIAKLGPGQERSAMEKIENLYRRFN
                     PGYTFEYQFMDQDFLAVYAAERRVGILSRYFAGLAIFISCLGLFGLATFTVERRQKEI
                     GIRKVLGSGTFNIFFLLSKGFIRLILIAIIIALPASYLLTKDWLDSFAYRIDLSIWYF
                     LTAGCLALTIAWLTVAMQALKAAYSNPLKSIRTE"
     gene            1591..>2631
                     /locus_tag="VKU83_02440"
     CDS             1591..>2631
                     /locus_tag="VKU83_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLI92435.1"
                     /translation="MQNKIDKTNVEDIVELNRVQEGMLYHYLQDENRNLYNVQLSFRI
                     NGFLDRAILQEALNAVQSAHQALRSVFRWEKLNKPVQVILYSCPTSIGYTDLSGRDEA
                     TGDLLAEYSLRKDWDQRFDLTELPLRLQIIRLGEQSFVLSITHHHILYDGWSTAIFLK
                     DLFYNYNQLVNKRAPLPGQQPSFKDVCLAIKRNNGIDNGNEFWPDYLKGYQLRSAFAR
                     KSLSAGPSDGIQKKVLTIGNIDLDTICRSFNLTKAVIIYATYAIFRQKWQGISDIVIG
                     TTVSSRSAAVRGQEHTIGNFINTIPMRLGGVEDRSFYEVITQLNRELLLRNQFVETSY
                     SDIKQLLRLKPAD"
BASE COUNT          685 a          592 c          666 g          688 t
ORIGIN      
        1 ccgcaagggc ggccacgagg ctgaaggaag tgggtataaa aaaggtagtc ggcgccttgc
       61 gaagggatct ggcgcttcaa tatctggcag aagctgtaat gatggcagtt tttgcctttt
      121 tggtcgctat cggcgttgtg atcgcattgc tgccgcagtt caatatactg acaggaaagc
      181 atcttgccct tcaatttaat ccgaatgtga tcctatcggt gatcggcatt ttactattca
      241 caggagtagc agccggcagc tatcctgcct tttacctttc gggattccga ccggtggcta
      301 tcttaaaaaa caagctgaag tcttccatag gtgaggtcat tgccaggaaa gggctggtca
      361 tatttcaatt taccttgtcg atcattctga tcgttgctgt attggtttgc aaccggcaga
      421 tcgaatatat ccagacgact aacctgggct atagccgcga taacgtagtc ttaataaaaa
      481 tggaaggcga catttcaaaa aatcttgaat ccttcctgac ggaattgaaa agcacgcccg
      541 gcgtgctcaa tgcttcggct atgagcggca atattacgga aagcgggaat agtacggggc
      601 cgggcggggt cacctggcgc gggagggacc ccaaagatgc cacctccttc gagtaccggg
      661 aagtaaataa tgactttgtc gaaacgctcg gaatgtccat ggcggaaggc cgtagttttt
      721 cccgggcata ccccaatgat acttccggca tcatctttaa tgaaacagcg atcaaagcca
      781 tggggcttaa ggaccctatt ggccaggcag tgacgtataa cggaacacag atgcatatta
      841 tcggggttgt gaaagacttt catttccagt ccttccatga ggcaataaag cctcttttgc
      901 tcgtagcggt tccggcagaa aacgatagga tcatcgctaa actcgggccg ggtcaggaac
      961 gttcagccat ggaaaaaata gagaatctat atagaagatt taaccccggc tacacattcg
     1021 agtaccaatt catggatcag gatttcctgg cggtgtacgc tgctgaaagg cgggtaggga
     1081 tattgtcgag atattttgcg ggcctggcta tcttcatatc ctgtttgggg ttattcgggc
     1141 tggccacctt taccgtggaa agaaggcaaa aagaaatagg gatccgcaag gtgcttggct
     1201 ccggtacatt caatatcttt ttccttttat caaaaggctt catccgcctg atacttatag
     1261 ccatcatcat tgcattaccc gcaagttacc tccttaccaa ggactggctc gatagctttg
     1321 cgtatcgcat cgatctgagc atctggtact ttttgactgc aggatgtctg gcattgacaa
     1381 tagcctggtt aacggtagcc atgcaggcgt tgaaggcggc atactccaat ccattgaaaa
     1441 gcatcaggac ggagtagtcg cattcccctc tttcaggtga tataaaatac agaaaactat
     1501 tgtgttgcga aacatcacct gcccatctga cggcagggtg atcgctattt gtgtctacgc
     1561 cgccacttcc ggcggcataa atgactcgct atgcaaaata agatcgacaa gacaaatgtc
     1621 gaagacattg ttgaactgaa tagggttcag gaagggatgt tgtaccatta cctgcaggat
     1681 gagaacagga atttgtataa tgttcaattg tctttccgga taaatggctt tctggatcgt
     1741 gcgatactgc aggaagcatt gaatgcggta caatctgccc atcaggcatt gaggtcggtg
     1801 ttccggtggg agaaattgaa taagccggtc caggtgatcc tttacagctg tcctacgtcc
     1861 atcggatata ctgatctttc cggcagggat gaagcgaccg gcgatcttct ggcggagtat
     1921 agcctcagaa aagactggga tcaacggttc gatctcactg aacttccgct gcgtttgcag
     1981 atcatccggc tgggtgagca gtcgtttgtt ttatcaatta cccaccacca tattctctat
     2041 gacggctgga gcactgctat ttttctgaag gatctttttt acaattacaa ccagctggtg
     2101 aacaaacggg ccccgttgcc tggtcaacaa ccctctttca aggacgtttg cctggccatt
     2161 aaacggaata acggcatcga taatgggaat gaattctggc cggattattt aaaaggctat
     2221 caactaaggt cggcttttgc aagaaaaagc ctgtctgccg gacctagtga cgggatccaa
     2281 aaaaaggtgt tgactatcgg gaatatcgac ctggatacca tctgccgttc gttcaacctt
     2341 acaaaagccg tgatcattta tgcgacctat gccatattcc ggcagaaatg gcagggcatt
     2401 tcggatatcg ttatcggcac tactgtttcc agccggagtg ctgctgtaag aggtcaggag
     2461 catacgatcg gcaattttat caacacgatt cccatgaggc ttggcggggt agaagaccgg
     2521 tccttttatg aggtgataac tcagttgaat agggaattgt tgttgaggaa tcaatttgtc
     2581 gaaacctctt attccgacat caagcagctg ttgagactaa aacccgcgga t
//
LOCUS       DATGKE010000139         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1313588, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000139 DATGKE010000000
VERSION     DATGKE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313588"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VKU83_02445"
     CDS             <1..285
                     /locus_tag="VKU83_02445"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HLI92436.1"
                     /translation="SLAEQILEKIPRKKGWVNHISDNLEDLEILSERVDPAPGTHRIE
                     YSSAIDTIEIVHTAHSRRGFASGAVLAAEFLQGKRGIYQMSDVLGFKQQL"
     gene            297..707
                     /locus_tag="VKU83_02450"
     CDS             297..707
                     /locus_tag="VKU83_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLI92437.1"
                     /translation="MLSKKTQYAFQALMYLAEKENGEPVLIAEIAKKKKIPLKFLENI
                     LLELKNADILDSKKGKGGGYFFAKNPKDVPLATVMRLLDGPISLLPCVSLYFYERCKN
                     CDEKTCGLHIVMSQVRDANLKILEKKTIADLAHG"
     gene            complement(733..1590)
                     /locus_tag="VKU83_02455"
     CDS             complement(733..1590)
                     /locus_tag="VKU83_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLI92438.1"
                     /translation="MKSSLAYLPAARRGELERVKEIIIDAVQPEKVILFGVYGSAQEA
                     EIFSGLPEGLSAYDLLVVTRNSERRSDYQLQDMIENRCKDSRAVTILVHDIGYVNSRI
                     AEGQCFFAAVRREGILLYDSGGSALVQPGETNLEQVLRTAEQDFARWCRQARAFLRSA
                     QVMLDGGEWKVALFMLHQAAEQMYQAILLTFMGYKPTTHNLDKLRRYTNRFSIELALL
                     FPRSEGEEDRLFRLLLSGYVEARYKDGFHVAEPDARLLTSRVGRLLEIGERVCRNRML
                     ALEKQLAKL"
     gene            1734..2126
                     /locus_tag="VKU83_02460"
     CDS             1734..2126
                     /locus_tag="VKU83_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLI92439.1"
                     /translation="MHFLGKNLRYLRKQSAKTQSEIASLIQKGQTTIGNWENGISEPS
                     LNELLVISNYFDIPVDTLLKIDLMETQVRPVPGSIGAARPYLPSDKEPLKTEDPGITM
                     AYVLKELKDVQQKIELLNSRLPKQQDKE"
     gene            complement(2132..>3177)
                     /locus_tag="VKU83_02465"
     CDS             complement(2132..>3177)
                     /locus_tag="VKU83_02465"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLI92440.1"
                     /translation="VQEVLMGCVLQGNLGQAPARQAAKGAGLPDEVVCTTVNKVCASG
                     MKAVAQGAQSISLGDAEIVVAGGMESMSNVPFYAAQMRWGNKYGNSTLIDGLAKDGLT
                     DAYDGLAMGNAAELCASTCGISREEQDAFAISSYRRSQAAWAEGKFAEEVVPVAMPQR
                     KGEPVVVAKDEEPYNVKFEKIPGLKPSFQEDGTVTAANASTLNDGAAALVLMSADKAR
                     SLGLQPLARIASYADAEQAPEWFTTTPSLAVPKAVAKAGLEMKDIDYWELNEAFSVVG
                     IENTRRMKLDAERVNVHGGAVSIGHPLGASGARILVTLIHILRQQKARWGAAGICNGG
                     GGASAMVIENLDL"
BASE COUNT          746 a          975 c          762 g          694 t
ORIGIN      
        1 tcactcgccg agcagatcct ggaaaaaatt ccccgtaaaa aaggatgggt caatcatatt
       61 agtgataact tagaagactt ggaaatcctg agcgagcgcg tcgacccggc gcctgggacc
      121 caccgcatcg aatacagttc ggcgatcgat acgatcgaga ttgtgcatac agcccatagc
      181 cgtcggggat tcgcgtcggg ggccgtcctg gctgctgaat ttttgcaggg taagcgagga
      241 atttaccaaa tgagcgacgt gctcgggttt aaacaacaac tgtaggaggt taagcaatgc
      301 tttccaaaaa aacacaatac gcttttcagg cgctgatgta cctggccgaa aaagaaaacg
      361 gcgaaccggt tctcattgcc gagatcgcca aaaaaaagaa gatcccgcta aaatttctcg
      421 aaaatattct gctcgagctc aagaacgccg atattctcga cagcaagaaa gggaaaggcg
      481 gcggctattt ttttgccaag aaccccaagg acgtcccgct ggccacggtc atgcgcctgc
      541 ttgacggccc catctctctc ctgccctgcg tctcacttta cttctatgaa cgttgcaaga
      601 actgcgacga aaaaacttgt gggctccata tcgttatgtc ccaggtgcgg gacgccaatc
      661 tgaagatcct ggaaaaaaag acgatcgccg accttgccca cggctaagat gatactgcct
      721 gcacaccctc ggctacaact tcgccagctg cttttccagc gccaacatcc tgttccggca
      781 aaccctttcc ccgatctcga ggagtcgccc aacccggctt gtcaataacc gtgcatccgg
      841 ctcggccaca tgaaaaccgt ccttataacg cgcttcgaca tagcccgata gcaatagccg
      901 gaacaaccgg tcttcctctc cttcgctcct gggaaaaagc agcgcaagct cgatcgaaaa
      961 ccggttcgtg tagcgccgca atttgtccag gttatgggtc gttggcttat accccatgaa
     1021 tgtcagcagg atggcctgat acatttgctc ggccgcctgg tgcaacataa aaagcgccac
     1081 cttccactcc ccgccgtcca gcatcacctg ggcgcttcga agaaaggccc gggcctggcg
     1141 gcaccagcgg gcaaaatcct gttcggccgt ccgcaggact tgttccaggt tggtctctcc
     1201 cggctggacc agtgccgatc caccggaatc gtacaaaaga attccctcgc gtctgacggc
     1261 ggcaaagaaa cactgtcctt cggcaatgcg gctattgacg taaccgatat cgtggaccag
     1321 gatcgttacc gccctgctgt ccttgcagcg gttttcgatc atatcttgca actgatagtc
     1381 ggatcgccgc tcactgttcc tcgtcaccac cagcaggtca tacgcggaaa gcccctccgg
     1441 cagcccggaa aagatttccg cttcctgcgc ggagccataa actccaaaaa gaatgacctt
     1501 ttccggctga acggcatcaa taatgatctc ctttactctc tccagttccc cgcgccgcgc
     1561 ggccggcaaa taggccaaag aacttttcat gtgggtggat gaggtattca tagcaaaagg
     1621 cttgtaagtc ccaagatatt tggctaactt tgccaagtta atccacaaat ctataaattt
     1681 gctaaagata ccaataaaaa agatactaac atctttggca ctattcgaac aacatgcatt
     1741 ttttaggaaa aaatcttcgg tatttaagga aacaatcggc caaaacccaa tctgagattg
     1801 cgtcgctgat tcagaagggt cagacaacca ttggcaactg ggagaatggg atcagcgagc
     1861 caagtttgaa tgaattgctc gtcatcagca attattttga tatcccggtg gatacccttc
     1921 ttaagatcga tcttatggaa acccaggtcc ggccggtccc gggatcgata ggtgcggcgc
     1981 gaccctacct gccgtcagac aaggaaccac tcaaaacgga agatccggga atcaccatgg
     2041 cttacgtgct gaaggagctc aaagacgtcc agcagaagat cgaactattg aattcgcgcc
     2101 tcccgaagca gcaggacaag gaatagcaac gctacaaatc caggttttca atcaccatcg
     2161 ccgacgcccc gccgccgccg ttacaaatgc ccgctgcccc ccacctggcc ttttgctggc
     2221 ggaggatatg gatcaacgtt accaggatcc tcgcgccgct cgcgccgagg ggatgcccga
     2281 tcgacaccgc gccgccgtgc acattcaccc gctccgcgtc cagtttcatt cgccgcgtgt
     2341 tttcgatccc tacgacgctg aatgcttcat tcaactccca atagtcgata tccttcattt
     2401 caagtccggc ttttgcgacc gccttgggta ccgccagcga gggagtcgtc gtaaaccatt
     2461 ccggcgcctg ttccgcgtca gcgtaggaag cgatcctggc cagcggctgc aaacccaggc
     2521 tccgggcttt gtcagcgctc atcaatacca atgccgccgc accgtcgttc agggttgatg
     2581 cattggctgc cgtgaccgtc ccgtcctcct ggaacgaagg cttcaggccg gggatctttt
     2641 cgaactttac attgtatggc tcctcgtctt tcgccacgac taccggctcg cccttgcgct
     2701 gcggcatggc gaccggcacc acttcttcgg caaacttgcc ttcggcccac gccgcctgcg
     2761 agcgacgata gctcgaaatg gcaaatgcat cctgttcttc gcgactgatt ccgcaagtcg
     2821 aagcgcaaag ctccgctgca tttcccatcg ccaatccatc gtaagcgtcc gtcaagccgt
     2881 ctttcgccaa accgtcgatc agcgtgctgt tgccatattt gtttccccac cgcatttgcg
     2941 cggcgtaaaa agggacgttg ctcatgcttt ccattcctcc cgccacgacg atctctgcgt
     3001 cccccagcga gatgctttgc gcaccctggg ccaccgcctt catgccgctg gcgcaaacct
     3061 tgttaaccgt tgtgcatacc acctcatccg gcaaccccgc acctttcgcg gcctgccgcg
     3121 ccggcgcctg gccgagattt ccctgtaaca cgcaacccat caatacctcc tgcacca
//
LOCUS       DATGKE010000140         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_276015, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000140 DATGKE010000000
VERSION     DATGKE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276015"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VKU83_02470"
     CDS             <1..1134
                     /locus_tag="VKU83_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLI92441.1"
                     /translation="ENRTYDQVLGDVASGNGDTSLVLFGNRVTPNQHALVNQFVLLDN
                     FYVNAEVSADGHNWSMGAYATDYLEKTWPTSYGGRGGDYDAEGNRAIANNKGGFIWDD
                     CQRAGVSYRSYGEFGKDGGPKEDGQPNPLITGLRGHYCASFTGWDLTVPDTTRFTQWK
                     ADFDSLMAAGGLPRLNVIRVGNDHTAGLRRGSPTPFAYVADNDLAVGEFVEYLSKSPI
                     WKESLVVILEDDAQNGPDHVDAHRSPCYLAGGLVRHHFVDHTLYTTTSVLRTIELILG
                     IPPMTQYDASATTMWRCFSSQPDLTPFRALPANVNLLDKNTAVNEWSKKSAGLDFARE
                     DRVPDRLFNEILWKGIKGDRAPVPVPSRAAFVKVVKAGVADDD"
     gene            complement(1165..1503)
                     /locus_tag="VKU83_02475"
     CDS             complement(1165..1503)
                     /locus_tag="VKU83_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92442.1"
                     /translation="MKKLFVLAAIVSGSLIASKSDAQVYVRGHFGRVGVGVGVPDCHD
                     PYYRGPVGYGAVPRYEYYPGAEYYPGYQYYDYPVWNGHFRDRIYYEHYRPLFFREHPY
                     YRSFRRGRRW"
     gene            complement(1675..2022)
                     /locus_tag="VKU83_02480"
     CDS             complement(1675..2022)
                     /locus_tag="VKU83_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92443.1"
                     /translation="MNRNFRPRKIARIITMVIVGILGFSLIVMLLWNALMPDIFGMRL
                     ISYWQALGLLILSKILFTGMRGGPGRGWRKEAIRDKWANMTPEERERFKEEWSRRCGK
                     PFTGNPPGAPTQA"
     gene            complement(2033..>2659)
                     /locus_tag="VKU83_02485"
     CDS             complement(2033..>2659)
                     /locus_tag="VKU83_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI92444.1"
                     /translation="ISLKYFLRPAVFLNRMEDLLTIEPRMTSPPGKNIASVVSRFGKR
                     LLGFIRQRVDNEADAEDILQDVWYQLTRAVDTEPIEQISAWLFAVARNRIIDRYRKHR
                     LVSLQPLLHDDEEPASRDLAGLIPDDGDPETVHLRSLFWKTLQESLAELPEEQRLVFV
                     WNELEGIPFNEIAELTGEKLNTLISRKRYAVLFLRERLLTLYHEIIEH"
BASE COUNT          560 a          735 c          780 g          584 t
ORIGIN      
        1 gaaaaccgga cctacgacca ggtcctcggc gatgtcgcca gcggcaatgg cgatacgagc
       61 ctggtgttgt tcgggaatcg ggtgacgccg aaccaacacg cattggtgaa ccagtttgtc
      121 ctgctggata acttctatgt aaacgcagaa gtcagcgccg acggccacaa ctggagcatg
      181 ggggcttacg ctaccgatta cctcgaaaag acctggccga cctcctatgg cggaagggga
      241 ggtgactatg acgcggaagg gaaccgcgcc attgccaata acaagggcgg gtttatctgg
      301 gacgattgcc agcgtgcggg ggtgagctac cgcagctatg gcgaattcgg caaggatggc
      361 ggcccgaagg aggacggaca gcccaacccg ctgatcaccg gtctgcgcgg gcattattgt
      421 gcttccttca ccggctggga cctgacggtg cccgacacga cgcggttcac gcaatggaag
      481 gccgatttcg attcgttgat ggccgcgggc ggcctgcccc gcttgaacgt catccgggtc
      541 ggaaacgatc atacggccgg gttgcgccgc gggtcgccaa cgccttttgc ctatgtcgcc
      601 gacaatgacc ttgccgtagg agaatttgtc gaatacctta gcaagagccc gatctggaag
      661 gaaagtcttg tcgtcatcct ggaagacgac gcgcagaatg gaccggacca cgtcgacgcg
      721 catcgcagcc cttgctatct cgccggcggc cttgtccggc atcacttcgt ggatcacacc
      781 ctgtacacga caacgtccgt gctgcgtacg atcgagctga tcctcgggat accgcccatg
      841 acccagtatg acgcatcggc gacgacgatg tggcggtgtt tttcttcgca gccggatctg
      901 acccctttcc gtgcattgcc ggcgaatgtc aacctgctgg ataagaacac ggcggtcaat
      961 gaatggtcga agaaatcggc cggccttgac tttgcgcggg aggaccgggt cccggatcgc
     1021 ttgttcaacg agatactctg gaagggcatc aaaggcgacc gggcgccggt gccggtgccc
     1081 agccgcgcgg cttttgtgaa ggtggtgaag gccggcgtcg cggatgatga ctgagtcggt
     1141 tggtccgccg gctacgcttg tcggttacca ccttctgccg cggcggaacg aacgatagta
     1201 tggatgttcc ctgaagaaca gcggacgata atgctcgtag tagatgcggt cgcggaagtg
     1261 gccgttccat acggggtagt cataatattg ataaccggga tagtattcag cacccgggta
     1321 gtattcataa cgcgggacgg cgccatatcc gacgggtccc cgatagtacg ggtcgtggca
     1381 atcgggaacg cctacgccga caccgacacg gccgaaatgg ccgcggacat acacctgtgc
     1441 gtcgcttttc gacgcgatga gggaaccgct tacgatggct gcaaggacga atagcttttt
     1501 catagctgta tttttgaagt tcgcgaaatt tttgaagttc gcggatcgga tttttgtttc
     1561 cgcatccttg ctatttggat ttgaaatgag cccgggaatt taataagcgt ttgttaaatc
     1621 gggcaactgc ctgtgcgacc ggctgttact gccggatcgt ggccgtcgcg cgcatcacgc
     1681 ctgcgttggc gcgccagggg gattgccggt gaacggcttc ccgcatcgcc ggctccattc
     1741 ttctttgaac cgctcccttt cttccggtgt catatttgcc catttgtcgc gaatcgcctc
     1801 ttttcgccag cctcgtcccg ggccgccgcg catcccggta aataatattt tagataagat
     1861 gagcagtccc aacgcctgcc aataactgat cagccgcatc ccgaagatgt ccggcatcaa
     1921 ggcgttccaa agcagcatga cgataagact aaatccgaga atgcccacga tgaccatggt
     1981 aatgatgcgc gctattttac gcggcctgaa attcctgttc ataaatttta ttttaatgtt
     2041 cgataatctc gtgatagagg gtcagcagtc tttcccgaag aaagaggacc gcataacgtt
     2101 tccgggagat cagcgtattc agtttctccc cggttagctc agcgatctcg ttgaatggga
     2161 tgccttccag ttcgttccat acaaatacca gcctttgctc ttcgggcagc tccgccagcg
     2221 actcctgcag cgtcttccag aacagggacc ggaggtggac agtctcggga tcgccatcat
     2281 ccgggatgag acctgcgagg tcccggctcg ccggttcctc gtcgtcatgg agtaagggct
     2341 gcagcgatac caggcgatgt tttcgatagc ggtcgatgat ccggttgcgc gcaacggcaa
     2401 aaagccaggc gctgatctgt tcgatgggtt ccgtatcgac cgccctcgtc agctgatacc
     2461 agacgtcctg caaaatgtct tccgcgtccg cctcgttatc aactcgttgg cggatgaagc
     2521 ccagcagccg cttgccgaaa cggctgacga ccgaagcgat attttttccg ggaggcgaag
     2581 tcattcgcgg ctcaatggtc aaaaggtcct ccattctatt caggaagacg gccgggcgga
     2641 ggaaatattt taaagaaat
//
LOCUS       DATGKE010000141         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_479302, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000141 DATGKE010000000
VERSION     DATGKE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479302"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VKU83_02490"
     CDS             complement(<1..613)
                     /locus_tag="VKU83_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HLI92445.1"
                     /translation="MAAPSILFENADFIAVDKPAGLLSIPDREGREPSLKSQLRARHG
                     NIFTVHRLDRDTSGVIIFAKTEESHKYLSRIFEEHNVEKVYQGIVQGSPPQSRGRVDL
                     PIMEHPTKPGLMVINRRGKPSLTDYQVLETLGPYSQLQFQIHTGRTHQIRIHMQAIGH
                     PIACDELYGDGKPVFLSKIKRNYKLSLAEEAERPLLARLALHSL"
     gene            complement(616..1647)
                     /locus_tag="VKU83_02495"
     CDS             complement(616..1647)
                     /locus_tag="VKU83_02495"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLI92446.1"
                     /translation="MKQSPPRERPSLGKRKTDNFSKFYNKKRNSAIKEGLRQEKKAAK
                     KEREQAIERHFEDRRQSKVMKRDAVPAKVDRSHAPSASSAASLPLNKYISHSGICSRR
                     DAAELVRQGKVTVNGKPVTEPGTKVQPTDIIKIGNKKLTISHNFVYILLNKPKDYITT
                     TEDPQGRRTVLDLIRTATTERVFPVGRLDRNTSGVLLFTNDGDLAQKLAHPSHQIRKI
                     YHVTLDRPLTKADFDAIASGAITLEDGPASVDDLAYADPKDRSQIGLEIHSGRNRIVR
                     RIFEHLGYDVRALDRVMYAGLTKKNVQRGHWRLLNEKEVRVLKYLNSSIKGSPGRAKD
                     LAPGQKNPQ"
     gene            1969..>2608
                     /locus_tag="VKU83_02500"
     CDS             1969..>2608
                     /locus_tag="VKU83_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HLI92447.1"
                     /translation="MSVSKTSIRLTVTLLLVLSMNRSQAYSILAHEALIDSCWATSIR
                     PLLLAKFPGATEAQLKTAHAYAYGGAIAPDIGYFPFGSPFFTNLVHYVRSGDFVNALL
                     ADARDLNEYAFAIGFLSHYMADRYGHSLATNKSVPLLYPKVRKKYGNWVTYEENHLAH
                     KRVEFGYDVLQTVEGDYTKIAYHDFIGFEISDGLLERAFTEVYGLDIHKVFPV"
BASE COUNT          523 a          628 c          781 g          676 t
ORIGIN      
        1 ggagcgagtg gagggccagc cgggcaagca gtggccgctc ggcttcctca gcaagggaga
       61 gtttgtaatt ccgcttgatt ttggaaagaa ataccggttt gccgtcgcca taaagttcgt
      121 cgcaagcgat ggggtggccg atagcctgca tatggatgcg gatctgatgc gtgcgaccag
      181 tatgaatttg aaactgtagc tgtgagtaag gaccgagggt ctcgaggacc tgatagtcgg
      241 tgagggaagg tttcccgcgc ctgttgatca ccatgagtcc gggcttcgtc gggtgttcca
      301 ttatcggcag gtccacccta ccccgcgatt gtggcgggct gccctggacg atgccctggt
      361 agaccttttc cacgttgtgt tcttcaaaga ttctggaaag gtatttatgg ctttcttccg
      421 ttttggcaaa aatgataacg ccactggtgt cccggtcgag ccggtggacg gtaaaaatat
      481 tgccgtgcct ggcacgcagc tggctcttca aagaaggttc cctgccctcc ctgtcgggaa
      541 tgctgagcag gcccgcaggt ttgtcgacag caatgaagtc cgcgttttcg aagagaatgg
      601 aaggcgccgc cattgtcact ggggattttt ttgcccgggg gcaaggtcct tcgcccgccc
      661 tggggaaccc tttatggaag agttaaggta tttcagcacg cggacttctt tctcgttgag
      721 gaggcgccag tggccgcgct ggacgttctt ttttgtcagg ccggcgtaca ttacccggtc
      781 gagcgctcta acgtcatagc ccagatgttc gaagattcgc cgtacgatcc ggttgcgccc
      841 gctgtggatc tcgaggccaa tttgggagcg gtccttcggg tcggcatacg cgaggtcgtc
      901 gacagatgcc gggccgtcct cgagcgtaat ggcgccggag gcgatggcat caaagtcggc
      961 cttggtgagc ggtcgatcca atgtcacgtg atagatcttt cggatctggt ggcttgggtg
     1021 agcgagtttc tgcgccaggt cgccgtcgtt cgtgaaaagc aacacgccgg aagtattgcg
     1081 gtccagccgc cctacgggaa agacacgttc ggtcgtggcg gtgcggatca ggtcaaggac
     1141 agtgcgccgg ccctgcgggt cttcggtggt agtgatatag tccttgggct tgttgagaag
     1201 aatgtagacg aagttatgcg agatcgtgag tttcttgttg ccgatcttaa tgatgtcggt
     1261 gggctggact tttgtgccag gttctgtgac gggtttcccg ttgacggtca ccttgccttg
     1321 gcggaccagc tccgcggcgt cgcggcgcga acagatcccg gagtgggaaa tgtacttatt
     1381 caggggcagg ctcgccgcgg agcttgcgga aggggcatgg ctgcggtcga cctttgcggg
     1441 gacagcgtcc cgcttcatga ccttcgattg ccgccggtct tcaaaatggc gttcaatggc
     1501 ctgttcccgc tctttctttg ccgctttctt ttcctggcgc aggccttcct taatggcgct
     1561 gttgcgcttc ttattataga acttgctaaa attgtcggtt ttgcgtttgc ctaaagaggg
     1621 acgctcccgg gggggagatt gcttcatgtt tcgattttgc tgttttacaa cagtaatgag
     1681 aggcaaaggt acgatttgcg gtggataaaa gaaaaacgcc gcaatcttgc ggctattggc
     1741 gttctcctgg tgattcaggg tccgctgttc ctgccgggtg atatgactgc gcaaagctag
     1801 cggccgtaaa cccggaaatg gcaatgtgag tggcgattaa gtgtttgatt ttcaaaatag
     1861 tagactttaa agaggatgcc ggtgacgacg ggaacctttc gtttggcgcg tttttagtga
     1921 ggtagaaagt tgttgctttt atgggacagc aaaatataat tcggctatat gtctgtatca
     1981 aaaacgagta ttcggctcac cgttaccctc ctgctagtcc tcagcatgaa ccgttcacag
     2041 gcatattcca tccttgcgca tgaagccctg atcgattcct gctgggcgac ttccatccgg
     2101 ccgctgctgc tggctaaatt tccaggggcg acagaagcac agttaaagac tgcgcacgcc
     2161 tacgcatatg ggggggccat cgctcccgac atcggctatt ttcctttcgg cagcccattt
     2221 tttacaaacc tcgtccacta tgtgaggagc ggggactttg tgaatgctct gttagcggat
     2281 gcccgggacc tcaatgaata tgctttcgcc atcgggtttc tatcccatta tatggctgat
     2341 aggtacggac attccttagc caccaacaaa tccgttccgt tgctttatcc caaagtgcgg
     2401 aaaaaatatg ggaactgggt gacctacgaa gaaaatcatc tcgcacacaa gcgggtagag
     2461 ttcggttatg atgtattgca gactgtggaa ggcgattaca cgaagatcgc gtaccatgat
     2521 tttatcgggt tcgagatatc cgacggattg ctggaaaggg ctttcacgga ggtttatggg
     2581 ctggatatcc ataaagtttt tccggtct
//
LOCUS       DATGKE010000142         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_275323, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000142 DATGKE010000000
VERSION     DATGKE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275323"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VKU83_02505"
     CDS             <1..421
                     /locus_tag="VKU83_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92448.1"
                     /translation="RSCNNYPVEGADRGFIIYGTGGTLVNLGGGDYKIFDEKKKLVKE
                     VKSEVVPDPTDPVAASGNMDLYHFNNFIKTIRGEATLNAPVDEASKSVLLCHLANIAQ
                     RTSGTLHCDPSNGHILDNPAATALWRRKYQPGWELEV"
     gene            complement(452..1807)
                     /locus_tag="VKU83_02510"
     CDS             complement(452..1807)
                     /locus_tag="VKU83_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLI92449.1"
                     /translation="MRYAITLACLLAGSSLFAQSAGWTPLFDGKSLNGWKQVAGTAAY
                     SVEDGAIVGTTVAGSGNSFLVTDREFADFVLEMDVRIEDTGSNSGVQVRSHFDPAGQD
                     GKGLVYGYQYEIDPSSRRWTGGIYDEGRRGWLYPVSLNHSVQSAFKVGQWNQVRIECA
                     GPAIRTWVNGLATAYLVDTMDKKGFIGLQVHAINRAEQAGKKVYFKNIRIRKTQPGFF
                     PPGVYVVDNIPNDLTDYEKKSGWRLLFDGSSSKGWIGVGRKEFPPHGWVIHNGILMVE
                     AGKGEMSTNAGDIVTTRQYKAFDLSFQFRLTPGANSGVKYFLTLKEPNTGHAGIGLEY
                     QLLDDSLHPDAKLGRNGDRTLASLYDLIPAVKQPRFLHPVGEWNTGRIIVFPDNHVEH
                     YLNGVKVLEYERGSADFRALVAISKYKIYPNFGEAPEGHILLQDHGNEVSFRSIKIRE
                     L"
     gene            complement(1912..2023)
                     /gene="rrf"
                     /locus_tag="VKU83_02515"
     rRNA            complement(1912..2023)
                     /gene="rrf"
                     /locus_tag="VKU83_02515"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2167..>2481)
                     /locus_tag="VKU83_02520"
     rRNA            complement(2167..>2481)
                     /locus_tag="VKU83_02520"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          535 a          711 c          670 g          565 t
ORIGIN      
        1 caggagctgc aataattatc cggtggaggg agcagaccgg ggatttatca tttacggcac
       61 gggaggcacg ctggtcaatc tcggcggcgg cgactacaag atcttcgatg agaagaagaa
      121 gctggtgaag gaagtgaaat ccgaggtcgt ccccgatccg accgaccccg tggccgcaag
      181 cgggaatatg gacctttatc atttcaacaa tttcatcaag accatccgcg gcgaggcgac
      241 gctgaacgct cccgtggacg aggcatccaa gtctgtcctg ctttgccatc tggccaatat
      301 tgcccagcgg acgtccggca cgctccattg tgacccgtcg aacggccaca tcctcgacaa
      361 tccggccgcg accgcgctct ggcgccgcaa atatcagccg ggctgggagc tggaggtatg
      421 atccccgcct gggactgccc gatcccgggc tttatagttc cctgatcttg atgctccgga
      481 aactcacctc gttgccgtgg tcctggagca ggatgtgtcc ttccggggct tcgccgaaat
      541 tcggatagat cttgtactta ctgatggcga cgagagcgcg gaagtccgcg gaaccccgct
      601 cgtattccag gacctttacg ccattgagat agtgttccac atggttgtcg gggaaaacga
      661 tgatccggcc ggtgttccac tcgccaacgg gatgcaggaa gcggggttgc ttgacggcgg
      721 ggatgagatc gtagagggag gccagtgtcc ggtcgccgtt ccggccgagc ttcgcgtccg
      781 gatgaagcga gtcgtcgagc agctggtatt ccagcccgat ccccgcgtgc ccggtattgg
      841 gttccttaag ggttaaaaaa tatttcacgc cgctgttggc gcccggcgtc aaccggaatt
      901 ggaaggacag atcaaaagcc ttatactgcc gggtggtcac gatatcaccg gcgttggtgc
      961 tcatttcacc cttcccggcc tcaaccatca ggatgccatt gtggatcacc cagccatgcg
     1021 gcgggaattc ctttcgtccc acgccaatcc agcccttgct gctgctgcca tcgaacaaca
     1081 gccgccagcc gctttttttc tcataatccg tcagatcatt gggaatgttg tccaccacat
     1141 atacgcccgg cggaaagaag cccggctgcg ttttcctgat ccggatattt ttaaaataaa
     1201 cttttttgcc ggcttgttcc gcccggttga tggcatgaac ctgcagaccg ataaaaccct
     1261 ttttatccat cgtatccacc agataggcgg tggcaaggcc gttcacccag gttctgatgg
     1321 ccgggcccgc gcattcaatc cggacctggt tccattggcc caccttgaac gcgctttgaa
     1381 ccgagtggtt gagtgatacg ggataaagcc agccgcgccg cccttcgtcg tagatgccgc
     1441 cggtccaacg gcgggatgaa ggatcaatct catactggta accatatacc agtcctttcc
     1501 cgtcctgacc cgcgggatcg aaatggctgc ggacctggac gccggaattg ctgccggtat
     1561 cttcgatcct gacgtccatt tccaggacga agtcggcaaa ttcccggtct gtcacgagga
     1621 agctattgcc ggagccggcg acggtggttc ccacgatcgc gccgtcttcg acggaatagg
     1681 ctgcagtgcc ggcgacctgt ttccagccgt tcagtgattt gccgtcgaag agcggcgtcc
     1741 aaccggccga ttgtgcgaag agggagctgc ccgccagcaa gcaggcaagg gttatcgcgt
     1801 atctcatggc ctaaagttaa gtcctcatgg catccggcca aaaaaaaagc cctgtcacga
     1861 tgaatgacag ggctcccgga acggcgtggc cgttacggac tcgagaaatg aatatggcag
     1921 ctacctactc tcccgcattg tggtgcagta ccatcggcca tgaggggctt aacttctctg
     1981 ttcggtatgg gaagaggtga acaccctcgg caaaaccacc ataagtcgat ctgctttgac
     2041 ccttgatctt ccggccaggg gtcgccgcta atatcatgac atatcgggaa gttgtaaatc
     2101 gttgtaacca gactccgtct atgtttatac acgtcgtgta tatccgcgga cttcgtccat
     2161 tttaaaaaaa ttaaagctta cgggtaatta gtactactcg gctttactgt taccagtttt
     2221 agacctgtag cctatcaacg tcatcgtctc tgacgaccct taaaagaaaa ctcatctcgt
     2281 ggaaggtttc acgcttagat gctttcagcg tttatcctgt ccgtacatag ctactcagcg
     2341 ctgcacctgg cggcacaact gatacaccag cggtacgtac gacccggtcc tctcgtacta
     2401 aggtcatgtc cactcaattt tctaacgccc atcacagata gggaccgaac tgtcttgcga
     2461 cgttctgaac ccagttcacg t
//
LOCUS       DATGKE010000143         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1123519, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000143 DATGKE010000000
VERSION     DATGKE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123519"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VKU83_02525"
     CDS             complement(<1..1023)
                     /locus_tag="VKU83_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92450.1"
                     /translation="MAPGSARNYRYVYLVAFLSVLYLAFLASSAQPGVFFSSDGGVKY
                     IVVKQLTEGHGFKYLYLPQPGWVQQIWANGYFPFHPPFLYPSPQGYLFVFPPAFQLIS
                     AFFYKHLGDAGLYLIPFVSTLLLWLGFVVLLKRCGVSPNRIAAGLFLLVFCSPLTIYG
                     ATYWEHMTAILLLFAGLVYLTRPPSGFLALMLGLCSGLAAWFRPEAMMMNALYGLAVC
                     WLFYRERQRGAILFLGGIAIGVGSFLLFNKIEFDAFFGIHSRQVLQDSVAGGVEVDPV
                     RNLIANNLINIRHFLFILLLIPAAILWVRHKKPLDLRTRLLMLIVLAYSLLTPFIVPN
                     DGGRQWG"
     gene            1149..>3698
                     /locus_tag="VKU83_02530"
     CDS             1149..>3698
                     /locus_tag="VKU83_02530"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92451.1"
                     /translation="MRQILTSLTFLVLLNLTLQAQMPPAKSGGQRQGPPPSIGRTFGK
                     VTDSAGQPLQATVIVLKSTVDPATKKKKLVLQKGMDTKANGEFNFEDLPIVTPLIVRI
                     SAIGYKTVDVPVTIIPAAPGGAAPAPAGQNSGSPYAGMPSFEKDLGAVRLAADVRTLS
                     AATVVAAPPTMKLELDKKVFNVEKNIVSAGGTGLDVMRNVPSVNVDIDGNVTLRGQTP
                     TIFVDGRPTTLTLDEIPADAIESVEVITNPSAKFDASGGGAGILNIILKKNRKKGYNG
                     SLSAGIDSHGAPNALAGFNIRQGKINVTANGLYNGMNGKTIGNTDRYTFLGDTTRLLQ
                     TDLQRNKGHFMFGQLGLDYFVSNKTTFSISYIKVHGEFGPRDVSQIETDSLSAPDKSV
                     TFATRSSNSTRVFDVTGGQFGMKHLFAKEGENWTADASLFDVHSHSNALYTTDYYSGA
                     PGSNPSEITQQQTLSSASPLFYTLQTDYTDPLGKNSKLEAGLRAAVQHLDNLANTYNN
                     DGAGHFLLDSLGVSNYKSTSTVYAAYVNYTSSIGKFSYALGLRAESSSYTGQLLNIVP
                     PQTFKNTYPLSLFPSVFVSQKFAHDQELQLSATRKISRPTFFQLIPYTDYSDTLNITR
                     GNPGLVPQFTQSFELTYMKTLAHSNTFLFSAYYKYTSNLITPYQDSGLNPVGKPVLIN
                     TYENANNSYNLGFEATATYNFTHWWDASLNVNVYNSHINTENLDQPSQNAMWSYFGKF
                     NSNFKLPDGFTIQLTSTFQSKTQLPVNTNQGMFGPPNSQTQASAQGFIKAFYGVDLAV
                     KYSFLKQNAASVTLSMSDIFRTRWSRQFSESAFFQQEFDRLKDPQLFRVVFA"
BASE COUNT          926 a         1016 c         1012 g          744 t
ORIGIN      
        1 accccattgc ctgccgccgt cattcggcac gatgaagggg gtgaggagag aatacgcaag
       61 aacgatcagc atgaggaggc gggtgcgaag atccaatggc ttcttgtgcc ggacccaaag
      121 aatagccgcg gggataagca ggaggataaa cagaaaatgg cggatattga tcagattgtt
      181 tgcaatgaga ttgcggaccg ggtccacctc gacgcccccc gcaacgctgt cctgcaagac
      241 ctgacggctg tggatgccga aaaaagcgtc gaactcgatc ttgttgaaaa gcaaaaagct
      301 gccgacgccg atggcgatcc cgccgaggaa aaggatggcc ccgcgctgcc gctctcggta
      361 aaaaagccag caaacggcga gcccgtacag cgcattcatc atcattgcct cggggcggaa
      421 ccaggcagcc agcccgctgc atagtccgag catcagcgcc aggaagccgg atggcggcct
      481 tgtcaggtag accagcccgg cgaacaaaag aagaatggcc gtcatatgtt cccagtacgt
      541 ggcgccgtag atcgtcaaag gactgcaaaa gaccaggaga aataggccgg cggcaatgcg
      601 gttcggggaa acgccgcagc gtttaagcag gacaacgaaa ccgagccaga gaagaagcgt
      661 tgaaacgaac ggaataaggt ataagccggc atcgccgaga tgcttgtaga agaatgcgct
      721 gatgagttgg aaagcaggcg ggaagacaaa gagataacct tgaggcgaag gatagagaaa
      781 aggcggatgg aacgggaaat agccgttcgc ccagatctgc tgtacccatc cgggttgggg
      841 cagatataga tacttgaacc cgtgcccttc ggtcagttgt ttgacaacga tgtatttaac
      901 accgccatcg cttgaaaaaa agacgccggg ctgtgcggag gaagcgagaa aggccaggta
      961 caggacagag aggaaagcaa cgaggtacac gtaccggtaa ttgcgggctg atcctggcgc
     1021 catgcgacga aattacaagg ttttgccgag agttttgcgc cgtttaccgt gttttttact
     1081 ccgtttgcag cattcatctg gacgggctcg gtttcggaag ctagctttgt gcatctaaat
     1141 ttacaactgt gagacaaatt ttgacgtcgc taaccttcct tgtccttttg aatcttactc
     1201 ttcaggccca aatgcccccg gccaagagcg gtggccaaag gcagggcccg ccgccttcga
     1261 tcgggcggac attcgggaaa gtaaccgatt ccgccggcca accgctgcag gcaaccgtaa
     1321 tcgttctcaa atcgaccgta gacccggcaa cgaaaaagaa gaagctggtt ttgcagaagg
     1381 gtatggacac gaaagcaaac ggggagttca atttcgagga ccttccgatc gtgacgccat
     1441 taatcgtccg gatctccgcc atcggctaca agaccgtcga cgtaccggtg acgatcattc
     1501 ctgcggctcc tggcggagcc gcacctgctc ccgccggtca gaacagcggg tcgccgtatg
     1561 caggcatgcc gtcattcgaa aaggatctgg gcgcagtcag gctcgcggcg gacgtccgga
     1621 cgcttagcgc ggccacggtc gttgcggcgc cgccgaccat gaaactcgag ctggataaga
     1681 aagtctttaa tgtggaaaaa aatatcgtca gtgccggcgg gacaggcctc gacgttatgc
     1741 ggaatgtccc ctcggtcaat gtcgatatcg acgggaatgt caccctccgc ggtcagacgc
     1801 cgaccatctt cgtggacggc cggcccacta ccctgaccct ggacgagatt cctgcggatg
     1861 ccatcgaaag cgtcgaggta ataacgaatc cttcggcgaa atttgatgcg tccggtggcg
     1921 gcgccggcat cctgaatatt attctgaaaa agaaccgcaa gaagggatat aacggcagcc
     1981 tgtcggccgg catcgactcg catggcgcgc ctaatgcgct cgcggggttc aatatccgcc
     2041 agggtaagat caatgtcacc gccaatgggt tgtacaacgg gatgaacggc aaaaccatcg
     2101 gcaataccga ccgttatacc tttctgggcg atacgacgag gctcctgcag acagacctgc
     2161 agcggaacaa gggtcatttc atgttcgggc aacttggcct ggattatttc gtcagcaaca
     2221 agaccacgtt ttccatctct tatatcaagg tccacggaga gtttggcccg agggacgtct
     2281 cccagatcga gaccgacagc ctgtccgcgc cggataagtc ggtaacgttt gcaacgcgga
     2341 gctccaattc gacccgtgta ttcgatgtga ccggcggtca gttcggaatg aagcatttat
     2401 ttgcgaaaga aggcgagaac tggacggcgg atgcgtcgct cttcgatgtc cattcgcaca
     2461 gcaatgcact gtacacgacg gattattatt ccggcgcccc gggttcgaac ccgtcagaga
     2521 ttacgcaaca gcagacgctc agcagcgctt ctccattgtt ttataccctc cagaccgact
     2581 acacagatcc attaggcaaa aactccaagc tcgaagcggg cttacgggca gccgtacaac
     2641 acctggacaa ccttgcaaac acctataaca acgacggtgc gggccatttt ctgctcgaca
     2701 gcctcggcgt aagcaattac aagagcacca gtaccgtcta tgcggcctac gttaactaca
     2761 ccagcagtat cgggaagttc agctatgcgt tggggttgag ggcggaaagt tccagctata
     2821 caggccagtt gctcaacatt gttccgccgc aaacgttcaa gaatacctat ccgctgagtc
     2881 tcttcccatc ggtctttgtc agccagaagt tcgcgcacga ccaggagctg cagttgagcg
     2941 ctaccaggaa gatctcgagg ccgaccttct tccagttgat tccttatacc gattactcgg
     3001 ataccctgaa tattacccgc ggtaaccccg ggctggtccc gcagttcacc caatcgttcg
     3061 agctgaccta tatgaagacc cttgcccaca gcaatacatt cttgttctcg gcctactata
     3121 agtacacctc gaacctgatc actccgtacc aggacagcgg cctgaacccc gtcggcaagc
     3181 cggtgctgat caatacctat gaaaacgcca ataactccta taaccttggt ttcgaggcaa
     3241 cggcgaccta caacttcacc cactggtggg atgcttcgct gaatgtcaat gtgtacaatt
     3301 cgcatatcaa caccgagaac cttgatcagc cgtcccaaaa tgcgatgtgg agctatttcg
     3361 gaaagtttaa cagcaatttc aaactgcctg acggtttcac gatccaactg acctcgacct
     3421 tccagtcgaa gacccagctg ccggtcaata cgaaccaggg catgttcgga ccgcccaatt
     3481 cgcaaacgca ggcgtcggct cagggcttca tcaaggcgtt ctacggcgtg gacctcgccg
     3541 ttaagtatag tttcctgaag caaaacgctg cgtcggtcac cctttcgatg tccgatatct
     3601 tccggacgcg gtggtcgcgt cagttcagcg aaagtgcttt tttccagcaa gaatttgacc
     3661 ggttgaagga cccgcagctc ttcagagtgg tttttgca
//
LOCUS       DATGKE010000144         5809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1205916, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000144 DATGKE010000000
VERSION     DATGKE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205916"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VKU83_02535"
     CDS             <1..709
                     /locus_tag="VKU83_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HLI92452.1"
                     /translation="RVPNYRDLTPQEEWDLLENHYNRVTDTCIPEEEAFRRLQRHLVK
                     AWKEAEAKNERYFPHEFLYKVVRSGDLEQYYEIDPKDSWILAAAEKDIPIVVPGWEDS
                     TTGNIFASYVIKNELKAGTVKSGIEYMTWLAGWYRQNSAGKGVGFFQIGGGIAGDFPI
                     CVVPMMYQDLEWHDVPFWSYFCQISDSTTSYGSYSGAVPNEKITWGKLDIHTPKFIVE
                     SDATIVAPLIFAWVLGW"
     gene            724..1227
                     /locus_tag="VKU83_02540"
     CDS             724..1227
                     /locus_tag="VKU83_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92453.1"
                     /translation="MNTNSEELYLEAEADIKNNHYAEAFKKYESILYEEPDSAQAHNS
                     MGWLYKTQFDDYAKAELHFQTAVKCDPLYPHSYFHIAALLMDMERFEELQQHLEKCLQ
                     IRTIDKSWVYGRHALSEELRRNYDKAIYYFERAILASQNDEKIKDYRQDIERCQMKTE
                     IWKRHAP"
     gene            complement(1520..2797)
                     /locus_tag="VKU83_02545"
     CDS             complement(1520..2797)
                     /locus_tag="VKU83_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLI92454.1"
                     /translation="MINTYTLIWIVVAWLMIAFFSGMEVAFMRANKLAIELKKKQGLS
                     SGIILSHFVDNPVEFIGSTLAGFNLFLVIFGLMVGETFLPFWNALVTKVHVDNSWVNA
                     IRLFAETMAASAFILVFGEFIPRALFRANSNAMLGFFAGTFDVLNRLLNPIAAFFSAI
                     AKWILVYVFNVRMDERKGTLTGADIDHFLQQTREKEDDVHDLNTDLFEAALALPKVRV
                     RECLVPRKEVEGIDINMAMEEVRKKFVDTRLSKLVVYEGNLDHVSGYIHQLDLFGNPA
                     QISAILHPIPAVPESMSATDLINKFSREKKSIAWVVDEFGGTAGIVTMEDLLEEIFGD
                     IRDEYDTEEFEEKKLSNDEYIFSGRLELDYINRKYGLDLPENGSETLSGYIINQHETI
                     PHLKDRIIIGDYEFEIASVSETRIEMVRLKVLK"
     gene            complement(2820..3395)
                     /gene="gmk"
                     /locus_tag="VKU83_02550"
     CDS             complement(2820..3395)
                     /gene="gmk"
                     /locus_tag="VKU83_02550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLI92455.1"
                     /translation="MSADHKIVIITAPSGAGKTSITRYLLGKYPDYLAFSVSAATRSP
                     RIYEKDGQDYYFLSMDSFKQKIQHNEFAEWEMVYEGKYYGTLKSELDRIWNSGRTPLL
                     DIEVKGAIHIREQYPQSTLALFIEPPSVDELRRRLEMRGTETIESLLARVNKASYEIS
                     FKQHFDKIIVNDDLQKACREAENAIREFLGI"
     gene            complement(3392..4696)
                     /gene="murA"
                     /locus_tag="VKU83_02555"
     CDS             complement(3392..4696)
                     /gene="murA"
                     /locus_tag="VKU83_02555"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLI92456.1"
                     /translation="MHSFEVIGGKRLKGDITPQGAKNEALQIISAVLLTPEKMTVANI
                     PDILDVNLLIELLGEMGVKTDRVTRDTCVFQADNIDIDYLKSDDFRSKSGRLRGSVML
                     AGPMLARFRKAFIPKPGGDKIGRRRLDTHIIGFEKLGVRFTYHPEEGLFHLDAPRLRG
                     TYLLLDEPSVTGTANIIMAAVMAEGMTTIYNAACEPYIQQLCKMLVRMGAKIRGIGSN
                     LLHIEGVSGLGGTEHRILPDMIEIGSFIGLAAMTQSEITIRNAGLEDLGVIPDKFRQL
                     GIRLELQGDDIHIPEQEIYEIGTFLDGSVLTVYDHPWPGFTPDLLSIVLVVATQARGT
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRAAVVGLAREQRLRGISMSSPDIRAGV
                     ALLIAALSAEGKSIIQNIEQIDRGYQYIDHRLRQLGAQITRI"
     gene            complement(4708..5430)
                     /locus_tag="VKU83_02560"
     CDS             complement(4708..5430)
                     /locus_tag="VKU83_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HLI92457.1"
                     /translation="MPSIENMEYNTTRNHLVMREYGRHIQKMIEYLLTLEDKEKRQRN
                     AYAVIELMGFLNPHLKNVEDFRHKLWDHLFLISDFRLEVASPYPIPTRETLKAKPKPL
                     RYPKRYPKLSHLGKNLEIVINKALAEENPDKRNGFANAVAYYMKLAYNNWHKEVVHDD
                     AIQSELSQITNGQLEFTNTPFVKGFRVNEGRDTGNVANGNRGNYQDYRNKRNQKFQQR
                     NNNRNIDRNNDRGGNKFKKKRY"
     gene            complement(5498..>5809)
                     /locus_tag="VKU83_02565"
     CDS             complement(5498..>5809)
                     /locus_tag="VKU83_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI92458.1"
                     /translation="QMSYSQVAVIDPTSDKTSDISYRTYPNPATNSLLFQFNNNQTGR
                     FVLQLVNTAGQIVRETAATLTGTNQIRMDLSPQPPKGLYFLRATDLTHDRSYTTKVFI
                     Q"
BASE COUNT         1281 a         1479 c         1621 g         1428 t
ORIGIN      
        1 gcgggtgccc aattaccggg acctgacccc ccaggaggaa tgggacctgc tggagaacca
       61 ttataaccgg gtaacggaca cctgtatccc tgaggaggag gctttccggc ggttgcagcg
      121 gcatcttgtc aaggcatgga aggaggcaga ggcgaaaaat gagcgatatt ttcctcatga
      181 attcctgtat aaggtggtga ggagcggcga cctcgagcag tattatgaga tcgatccgaa
      241 ggacagctgg atccttgccg cggcggagaa ggatatcccg atcgtggtgc ccggctggga
      301 ggatagtaca acgggcaaca tttttgcgag ttatgttata aagaacgaac tgaaggctgg
      361 cacggtcaaa agtggcatag aatacatgac ctggcttgca gggtggtacc ggcagaacag
      421 cgccggcaaa ggcgtaggct tttttcagat cggcggcggc atcgcgggcg actttccgat
      481 ttgcgtcgtg ccgatgatgt atcaggacct ggagtggcac gatgttccgt tctggagcta
      541 tttctgccag atctccgatt cgacgacgtc atacggatcg tactcggggg ctgttcccaa
      601 tgagaagatc acgtggggaa agctggacat ccatacgccg aaatttattg tggaaagcga
      661 tgccaccatc gtcgcgccgc tgatctttgc gtgggtgctg gggtggtaat aataacaaga
      721 actatgaata caaattccga ggagctgtat ctcgaggcgg aagcggatat caagaacaat
      781 cactatgctg aggctttcaa aaaatatgaa agcattctgt atgaggagcc cgacagcgcg
      841 caggctcata attccatggg ctggttatac aagacacaat tcgacgacta tgcgaaagcg
      901 gagctccact tccagacggc ggtaaaatgc gaccccctct atcctcattc ctatttccat
      961 attgcagcgt tgctgatgga tatggagcgg tttgaagagc tccaacagca tttggagaaa
     1021 tgtctgcaga tcaggacgat cgataaatcc tgggtctacg gccggcatgc tctatcggag
     1081 gagctgcggc gcaattatga caaggcaatc tattattttg aacgggcgat cctggcgagt
     1141 caaaatgacg aaaagatcaa ggattaccgg caggatatcg aaagatgcca gatgaagacg
     1201 gagatctgga agcgccatgc gccgtagcgc ctgcacgcag gctttggcgg ggccgccggc
     1261 tgcgaagctt ccttcctggg ctccctaaaa aaaacaagca ctccattaca gagtgcattg
     1321 tttactccta actgacctat gaaaaattta attgttcagg ttgataggac gaaggtggga
     1381 aaaaagatca ggacggggaa aaatgcgaag ccgattaacc acggcttaac attggttaac
     1441 ggcctggtag cgaagacgct ggaatgccct gccagcgcgg gtcccggcgc gcgcgtcccg
     1501 aaaatggttg gatgcgagac tatttaagga cttttagccg caccatttcg atcctcgttt
     1561 cgctgacgct cgcgatctcg aactcataat cgccgatgat gatgcggtcc ttcaggtggg
     1621 gaatggtttc atgctggttg atgatatagc cggagagtgt ttctgatccg ttttccggaa
     1681 ggtccaggcc gtatttccgg ttgatatagt ccagctcgag ccggccggag aagatgtatt
     1741 cgtcgttcga cagtttcttt tcttcaaatt cttcggtatc gtactcgtcg cggatgtcgc
     1801 cgaagatctc ttccagcagg tcttccatgg tgacgattcc cgcggtgccg ccaaattcgt
     1861 ctacgaccca ggcaatgctt ttcttctccc ggctgaattt attgatcagg tcggtggcgc
     1921 tcatgctttc cggaacggcc ggaatgggat gcaggatggc gctgatctgc gccgggttcc
     1981 caaatagatc gagctggtga atgtatcccg atacgtggtc caggttccct tcatagacca
     2041 ccagcttgct caaccgggta tcgacaaatt tctttctgac ctcttccata gccatattga
     2101 tatcgatccc ttcgacctct ttccggggca cgaggcattc ccggaccctt acctttggca
     2161 gcgccagcgc ggcttcgaac aggtcggtgt tgagatcatg cacatcgtcc tccttttccc
     2221 tcgtttgctg gaggaaatga tcgatgtcgg cgcccgtcag ggtccctttt ctctcgtcca
     2281 tgcgaacgtt gaaaacgtag accaggatcc atttcgcaat ggccgagaaa aacgcggcaa
     2341 tcggattcag gagccggttc agcacatcga aggttccggc gaagaagcca agcatcgcgt
     2401 tgctgtttgc ccggaaaagg gcccggggaa tgaattcccc gaagacaagg atgaaagcgg
     2461 atgcggccat cgtttccgcg aacagccgga tcgcgttcac ccagctgttg tctacatgaa
     2521 ccttcgtgac cagggcgttc cagaagggca ggaaagtctc gccgaccatc aggccgaaaa
     2581 tgacgaggaa caggttgaat ccggccagcg tgctgccgat gaactcaacg ggattgtcca
     2641 cgaaatggga caaaataatc ccgctgctca atccctgctt tttcttcagc tcgatcgcca
     2701 gtttgtttgc acgcatgaat gcgacctcca ttcctgaaaa aaaagcgatc attaaccagg
     2761 caaccacgat ccagatgagg gtataggtgt tgatcataat gttcgggctg ctaaaatact
     2821 caaattccga ggaattcgcg gatggcattt tccgcttcac ggcatgcctt ctgcaggtcg
     2881 tcgttaacga tgatcttgtc gaaatgctgt ttgaaggaga tctcgtaaga tgctttgttg
     2941 actcgggcaa gcaggctttc tatcgtttcc gtgccacgca tttcgaggcg gcggcgaagc
     3001 tcgtccaccg aaggcggctc gataaaaagg gccagggtgg actgtgggta ctgttcgcga
     3061 atatggatgg cgccctttac ctcgatgtcc agaagggggg tccggcccga attccagatc
     3121 cggtccagct cgcttttcag cgttccataa tattttcctt cataaaccat ctcccactcc
     3181 gcgaattcgt tgtgttgtat tttctgtttg aacgagtcca tcgagaggaa ataatagtcc
     3241 tggccatcct tttcatagat cctgggcgag cgcgtggctg cagagaccga aaacgccagg
     3301 taatcggggt attttcccag aaggtaccgc gttatggagg tcttacccgc gcctgaaggc
     3361 gcggtaatga tgacgatctt gtgatccgca ctcatatcct ggtaatttga gcgcccaact
     3421 gtcgcagccg gtggtcgata tactggtatc cccggtcgat ctgctcgata ttttggatga
     3481 tgctttttcc ttccgcgctg agcgcagcaa tcaacagggc tacgccggcg cggatatccg
     3541 ggctgctcat gctgatcccc cgaagccgtt gttccctggc cagtcccacc acagccgcgc
     3601 ggtgagggtc gcacaggatg atctgggcgc ccatatcgat gagcttgtcc acgaaaaaga
     3661 ggcggctttc gaacattttc tgatggatca ggacggtacc gcgggcctgc gtcgccacta
     3721 ccagcacaat gctgagcagg tcgggagtaa aaccgggcca gggatggtca tataccgtca
     3781 ggaccgaccc gtcgagaaag gtgccgatct catagatctc ctgttccggg atatggatat
     3841 catcaccctg caattcgagc ctgattccca gctggcgaaa tttgtcgggg atgacgccga
     3901 ggtcttcaag gccggcgttc cggatcgtta tttcgctctg ggtcatcgcc gccagcccga
     3961 taaaagatcc aatttcaatc atgtccggca ggatccggtg ttccgttcca ccgaggccgc
     4021 tgacgccttc gatatggagc agattgctgc cgattcccct gatcttcgcg cccatgcgga
     4081 cgagcatttt gcacaactgc tggatatatg gctcgcatgc cgcattatag atcgtcgtca
     4141 ttccttccgc catgacggca gccatgatga tattcgcggt tccggtcacg gaaggctcgt
     4201 cgagaagcag atacgtcccc ctgagccggg gcgcgtcgag atggaaaagg ccttcttcgg
     4261 gatggtaggt gaaccgcaca cccagtttct caaagccgat gatatgggtg tccagcctgc
     4321 gccggccgat cttgtctccg cccggttttg ggataaaggc cttgcgaaag cgcgccagca
     4381 taggcccggc cagcattacc gatccccgca gccggccgct cttgctacgg aaatcatcgc
     4441 ttttcagata gtcgatatcg atgttgtcgg cctgaaagac gcaggtatcc cgggtgacgc
     4501 gatcggtttt cacgcccatt tctccgagca attcgatgag cagattgacg tccaggatgt
     4561 ccgggatatt ggcgaccgtc attttttccg gggtcagcag cacggcgctg ataatctgta
     4621 gggcttcgtt cttggctccc tgcggagtga tatctccttt cagtcgcttg cccccgatga
     4681 cttcaaatga atgcacggtc ctcccggtta gtatcgtttc tttttgaatt tattcccgcc
     4741 ccggtcgttg ttccggtcaa tattgcggtt gttattgcgc tgttgaaatt tctggttccg
     4801 cttgttgcgg taatcctggt aattgccgcg gttgccgtta gcgacgttgc cggtgtcgcg
     4861 tccttcgttg acccggaaac ccttcacaaa cggagtattg gtgaattcaa gctggccatt
     4921 ggtgatctgg gaaagctcac tctggatcgc gtcgtcatga acgacttctt tatgccagtt
     4981 gttataggct aacttcatgt agtaggctac ggcgttggca aagccattac gcttatccgg
     5041 gttctcctct gcgagcgctt tattaatgac gatttcaaga tttttcccca ggtgcgaaag
     5101 ctttggatag cgtttgggat atcgcagggg cttcggtttg gctttgagtg tttcccgcgt
     5161 tggaatggga taaggcgacg ccacctccag cctgaaatca gagatcagga agagatgatc
     5221 ccaaagtttg tgacgaaaat cctcgacgtt cttcaggtgc ggattcagaa accccatcag
     5281 ctcgatgacc gcataggcat tgcgttggcg cttttccttg tcttcgagcg tgagcaggta
     5341 ctcgatcatc ttttggatgt gccggccgta ctcccgcatc accaggtggt tcctggtggt
     5401 attatattcc atgttctcga tactgggcaa atgataatga ttttatcggc aaatctacac
     5461 aaaaaacata gaatggcgat atttcagcgg ccggcgttta ttggatgaat accttggttg
     5521 tatagctccg gtcatgagtg aggtccgtgg cccgcagaaa atacaatcct tttggcggct
     5581 gtgggctcag gtccatcctg atctggttgg tcccggtcag cgtggcggca gtttcgcgga
     5641 cgatctgacc ggccgtattg accagctgca ggacgaagcg tcccgtctgg ttgttgttga
     5701 actggaacaa caggctgttg gtagccgggt tgggataggt acgataactg atatcgctag
     5761 tcttgtcgct ggtcggatcg atgacagcca cttgcgaata gctcatttg
//
LOCUS       DATGKE010000145         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1311948, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000145 DATGKE010000000
VERSION     DATGKE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311948"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1812
                     /locus_tag="VKU83_02570"
     CDS             253..1812
                     /locus_tag="VKU83_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLI92459.1"
                     /translation="MKLFFWPAAVLLGIVLLLSLTHDPRNRSHAKVPMSSIRDGGRLA
                     TTYCGACHITPDPSLLDSRSWEKGVLPEMGPRLGIFAYGFQLYPNSRRDTNISKGFYP
                     SQPLLSLDDWQHIIDYYTALSPDTLPAQRRPEPIAMTGLSLFDVRAPAMRYPMPATTY
                     LGFDTSAGERGISVYDYNYRKLYRFSPDLRLIDSTADQGGIVGMERQGREWVSCDIGE
                     LNPNNGKSGTLKRIESDGKGHWRIDSPAFYSPLARPVQAVAADINGDGRMDWVVCEFG
                     NLVGALSWLENKGDSGFERHIIRASPGAIHVIVNDYNHDGLPDLWVLFAQGDEGIFLF
                     TNLGNGRFRQQTLLRFPPSFGSSSFDLADFNGDGYADILYTCGDNADFSRVLKPYHGV
                     YIFLNDGHNHFSQRYFFPIDGCYKAVARDFDGDGDLDIATIAFFPDLAHQPQEGFVYL
                     ENLGDFRFRPYSLPQAQQGKWLTMDVGDVDGDGKPDIVLGNFSYFAPVTPAGVDFKRG
                     PPFLLLKNLSK"
     gene            1818..>2162
                     /locus_tag="VKU83_02575"
     CDS             1818..>2162
                     /locus_tag="VKU83_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLI92460.1"
                     /translation="MSVFPSWLIYALLSAVFAALTAIFAKVGLKDVNSDLATAIRTAV
                     VLLITWGIVLVRGNARGIPGLSRNNWIFLVLSAVATGLSWLFYYRALQLGRVSQVSVI
                     DKGSILFTILLSF"
BASE COUNT          438 a          569 c          622 g          533 t
ORIGIN      
        1 cgccgtactg acttacagcg acgggtcaag aagaaaggaa gaatgcggat acggatcttc
       61 tttcctgtcg gaatccggcc attttttgac gataaacggg ccggtaacgg gagtaaccat
      121 cacgaattcg gccggccaga cgcggacagc tttttaaaag gccctaacct gcaatattta
      181 ccaggaaccg gtaggcgtca ccgtttggtg tttacatttt cggtagtttt atagccttat
      241 ttcactgtaa cattgaagct ttttttttgg ccggccgccg ttttgctggg catcgtcctg
      301 ctgctgtcgc tcacacacga cccccgcaat cgttcgcacg cgaaggtccc gatgtcctcc
      361 atccgggacg gcgggcgact ggccacgacc tattgcggag cctgccatat aactcctgat
      421 ccgtcgttat tggatagccg aagctgggaa aagggcgtgc tcccggaaat ggggcccagg
      481 ttgggtattt tcgcctatgg tttccagctt tatcccaata gcaggcgcga cacgaatatc
      541 agcaagggct tctacccatc ccaacccctg ttaagcctgg atgactggca gcatatcatc
      601 gactattaca cggcgttgtc gccggatacc ttgcccgcac agcggaggcc ggagccgatc
      661 gcaatgaccg ggctatcttt attcgatgtc cgggcgccgg cgatgcgata cccgatgccg
      721 gctaccacct accttggctt cgacacgtcg gccggggaac ggggtatcag cgtatacgac
      781 tacaactaca ggaaattgta ccggttctcg ccagacctgc ggctgatcga ttctacagca
      841 gatcaggggg gaatagtggg gatggagcgg caggggcggg aatgggtcag ttgcgatatc
      901 ggggaattga acccaaataa cggcaaatcg gggaccttga agcggatcga gtccgacggc
      961 aaggggcatt ggcggatcga ttcgccggct ttctattcgc cgctggccag accggtgcag
     1021 gcggtggccg cggatatcaa tggggatgga cggatggatt gggtcgtctg cgaattcggg
     1081 aacctcgtcg gcgcattatc ctggctcgaa aacaagggtg acagcggctt cgaacgtcat
     1141 atcatccgtg cgtcgccggg tgcgatccat gtcatcgtga acgattacaa ccatgacggg
     1201 ttgccggatc tgtgggtcct cttcgcccag ggagatgaag ggatctttct ttttaccaat
     1261 ttgggaaacg gcaggttccg ccagcagacc ttattgcgtt ttccgccttc attcgggtct
     1321 tcttccttcg atcttgccga cttcaacggg gatggatacg cggatatcct gtatacctgc
     1381 ggtgataacg cggatttttc acgggtgctc aaaccttacc acggggtcta tatattcctc
     1441 aatgacggac ataaccattt ttcccagcgt tatttctttc cgatagacgg ttgttacaag
     1501 gcggttgccc gggatttcga cggggacggc gacctggata tcgccacgat cgcttttttc
     1561 cctgacctgg cgcatcagcc gcaagaaggt ttcgtttacc tggagaatct cggcgacttt
     1621 cggttccggc cgtatagcct gccccaggcg cagcaaggta aatggctgac catggatgtg
     1681 ggtgacgttg acggggacgg gaagcccgat atcgtgttgg gcaatttttc ctattttgca
     1741 ccggtcacgc ccgcgggggt tgactttaag cgcggaccgc cattcctgtt attgaagaac
     1801 ctttcaaaat gaaatctatg tcagtctttc ccagttggct gatttatgcc ttgctgtcag
     1861 cggtttttgc cgcgctcacc gccatcttcg ccaaggtggg attgaaagac gtgaattctg
     1921 acctggcaac ggcaatccgg accgccgtcg ttttactgat aacctggggg atcgtgctcg
     1981 ttcggggaaa cgcgcgggga attccgggct tatcgagaaa caattggatc ttcctggtgc
     2041 tttcagcggt ggccacgggg ctttcatggc tattttacta ccgggccctg cagctgggca
     2101 gggtctcgca agtgagcgtg attgacaagg gcagtatttt gtttacgatc ctcctctcct
     2161 tc
//
LOCUS       DATGKE010000146         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_278973, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000146 DATGKE010000000
VERSION     DATGKE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278973"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VKU83_02580"
     CDS             complement(<1..1133)
                     /locus_tag="VKU83_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92461.1"
                     /translation="MNDIEVLRPALSRLQWKFLVVGVVALIACAVGAVFNWTQFARSY
                     LLAYIFWLEIGLGCFSILMLHHMVGGRWGYVIRRPLEAGSRTIPLLALLFIPLYFAHP
                     RLYPVVTGADFKARYLNPPFFFGRTILYFALWLLFAYLLNKWSSVQDKDSSPLLIKRL
                     QNISAPGLLIYCFSITFLAVDWVMSLEPKFFSTIWGMIFVIMPALVGMSFIVIITTLL
                     AKYEPVSRVASPERFNDFGNLLLVFVMLWAYLSFAQFLIIWAGNLQDEIPWYTVRATG
                     GWAAIGLFIILFHFAVPFVLLLNRPVKRKMKVLAGVGAGLVVMTWVDLFWFIVPSFYP
                     LAPHISWMDIVATIGVGGVWLAMFVGQLKKKPLLPLRDPRFEGV"
     gene            complement(1130..1660)
                     /locus_tag="VKU83_02585"
     CDS             complement(1130..1660)
                     /locus_tag="VKU83_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLI92462.1"
                     /translation="MTGCRLDMHIQPKYKDYEYSKFFSDNRSERPPVPGTVARGELRT
                     DELLYAGKINGVPADVFPFPITAKDLERGRQRYNIYCTPCHDYTGNGNGMIVQRGFPP
                     PPSYHIDRLMKAPVGHFFDVMTNGYGTMYSYAARVSPEDRWRIAAYIRALQLSEHATR
                     DDVPSEELQKLEGSAQ"
     gene            complement(1782..2291)
                     /locus_tag="VKU83_02590"
     CDS             complement(1782..2291)
                     /locus_tag="VKU83_02590"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HLI92463.1"
                     /translation="MAEFLSAEELLESAKHAFEAGYTSTDAFSPFPVEGLSDVVGFHR
                     GKVAVLTFIGGIIGCCGGFFLQWWPNVVGYPLDIGGKPYDSWPAFIPITFELTILGAA
                     LAATFGMLALNGLPCPYHPAFNVPRFALASQDRFFLLIMADDPKFDLEETRRFLSEFN
                     PREVSEVER"
BASE COUNT          684 a          728 c          679 g          479 t
ORIGIN      
        1 actccctcaa accgcggatc gcgcaaaggc agcagaggct tctttttgag ttgcccaaca
       61 aacatcgcca gccaaacgcc cccgaccccg atcgtggcca cgatgtccat ccaactgatg
      121 tgaggcgcca gcggataaaa ggaagggacg atgaaccaaa aaagatcaac ccaagtcatc
      181 acaaccagtc ctgcaccgac gcctgccagc actttcatct tcctcttgac cggcctgttc
      241 aaaagaagga cgaaaggcac ggcaaagtgg aagaggatga taaacaggcc gatagcggcc
      301 catcctccgg tggcgcgcac ggtgtaccag ggaatctcgt cctgaaggtt tccggcccag
      361 ataatcagaa attgagcgaa tgacagatag gcccacagca tgacgaacac cagcaggaga
      421 ttgccaaaat cattgaagcg ctcgggcgaa gccacgcgcg aaacaggttc atacttcgcc
      481 agcagcgtcg taatgatgac gatgaatgac atcccgacca gcgcaggcat gatgacgaaa
      541 atcatgcccc agatcgttga aaagaatttc ggctcaagcg acattaccca gtccaccgcc
      601 aggaacgtaa tgctgaagca gtagatcagg agacccggtg cgctgatatt ctgcagccgc
      661 ttgatgagca gagggcttga atccttgtcc tgcacggacg accatttgtt caggagataa
      721 gcaaataaaa gccacaaagc aaaatagagg atcgtgcgcc cgaaaaagaa tggagggttc
      781 aggtatcgag ccttgaagtc cgctcctgta accaccgggt agagacgggg atgggcaaaa
      841 tagagtggaa tgaacagcaa cgccagtaga gggattgtcc tgctgccggc ctcgagaggc
      901 cgccgaatga cgtaacccca cctgccgccc accatgtgat ggagcatgag aatagagaag
      961 cacccgaggc ctatttccag ccagaaaata tacgccagaa gataggatcg cgcgaactgg
     1021 gtccagttga acaccgcccc gactgcgcag gcgatcaaag ccaccacgcc aaccaccagg
     1081 aacttccatt gcagacggct taaagcgggc cgaagcactt caatatcgtt cattgtgctg
     1141 atccttcgag cttctgaagt tcctcagacg gaacgtcatc ccgagttgca tgctcactta
     1201 attgcaaggc gcggatatag gctgcgatgc gccagcgatc ctccggcgat actctcgccg
     1261 catagctgta catcgtcccg taaccgtttg tcataacgtc aaagaaatga cccacggggg
     1321 ccttcatgag gcgatcgatg tgatatgacg gcggcggggg aaatcctcgc tgcacgatca
     1381 tgccattccc gttgcctgtg taatcatggc agggggtgca gtaaatgttg tatcgctgcc
     1441 gtccgcgttc caaatccttg gcggtgatcg gaaaagggaa cacgtcggct gggacgccgt
     1501 tgattttccc cgcatacagc aattcgtcag tccgcagttc tccccgtgcg acggtgcccg
     1561 gaacaggagg acgctccgac cggttatcgc tgaaaaactt gctgtattcg tagtccttat
     1621 atttcggctg aatgtgcatg tccagccggc agccagtcat gagtattcca caggcggcaa
     1681 taccaatcca ccccaggccg atcaagggtc gcagacctgc tgcttcgggc cggcgagatc
     1741 ccgatgacca tgaagaaaac gaaaaatgcc gctcacttct tttacctttc aacttcggat
     1801 acctcgcgag ggttgaattc cgataagaac cgtcgagttt cttcgaggtc gaacttggga
     1861 tcgtccgcca taatcagaag gaaaaaccgg tcctgcgatg ccagcgcaaa gcgcggcaca
     1921 ttaaaagcgg ggtgatacgg gcacggcagc ccgttgagcg ctaacatgcc gaaagtcgct
     1981 gccaacgctg cgccgaggat ggtcaactcg aaggtgatcg gaatgaaagc cggccagcta
     2041 tcataaggct tgccgccaat atcgagcgga tagccgacca cattcggcca ccactggagg
     2101 aaaaatccgc cgcaacaacc tatgatgcct ccaataaagg taagaacggc caccttgccg
     2161 cgatggaagc ccacgacatc ggacaatcct tcgactggaa agggtgagaa agcgtccgtg
     2221 gaggtgtaac cggcttcgaa ggcgtgtttg gcgctctcca gcagttcctc cgccgacaga
     2281 aactcagcca ttaacccgta cagccttttt tcattttcca tcacaagctg cctgttagag
     2341 gattcactcc ggttctccgg cagtaagcac accggagccg caaaatgcct cttatgttcg
     2401 cgctatactc tcctctgctg tttcacgcac caagccgcgc atctccgaca tcgagatcat
     2461 gggcaggaag cggataaaca gatagaacag ggtgaaaaac aaaccgatcg acccaagcaa
     2521 aacggcccaa tcccatcgcg tggggtcata catgccccac gaagatggca
//
LOCUS       DATGKE010000147         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_274208, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000147 DATGKE010000000
VERSION     DATGKE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274208"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VKU83_02595"
     CDS             <1..639
                     /locus_tag="VKU83_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLI92464.1"
                     /translation="TTGLAVLGYYLRLAHSVEYQTAANETRSIRLADGTTMSLEEWTT
                     VRVEAGYNRSSRAITLLTGKAHFDVIHDAGRPFRVDAGTVEIRDIGTSFTVTLANDSV
                     GVEVITGKVSVEDKRTRQTFALSAGQAANNYSLGGSALKFRNAPLSAVLDALQRRYGK
                     SILLADTTLAAKRLTVNLEGESFEEAIKVICASLDLISHEASNGIVLSKATR"
     gene            687..3302
                     /locus_tag="VKU83_02600"
     CDS             687..3302
                     /locus_tag="VKU83_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92465.1"
                     /translation="MASAVLWMFLLLLAPRLYAQDSTTLVTVTIIDQPFPAALAILEM
                     KIPYKFAYSNELARMQKNVSIRATAMPLPDFLQTLFRNTGLSYRIIGDQIVVQQATTA
                     PRITFSGYIRDARTGESLVGASVYAPGLRVGTLSNNYGFYSLTIPRTDSIGLEISYVG
                     YQPLDLQLSGAASRQFSFPLEHSAEQEAIGKLVVTSDRREDNVRKNQPALVELSTEMV
                     TAAPSISGSGDIIGSVEMLPGVQAGIDGTPGYFVRGGNAGQNLILLDDATLYNPSHIF
                     GLVGIFNPPAIKYASLLKGGFPASYGDHLSSVLDVVMKDGSNQQYGGTLQAGTVSSGA
                     TLWGPVQPGKSSFLISGRRSTTDLLLKPFLDSNYFSHYYFYDLNAKLNVELTPKDRLL
                     ASFYTGRDNNTYSSDTASLEGINYAMNYGNTAVTLRWNHQYSTRLFGHTTVEYNHYHQ
                     FLSAAQEGYFAQLYSGIRDMDAKSELGWWLSPAHTISGGVDYLHQTVFPSTLSGQVAA
                     VDSARNIVPSGIQPLSSNRLALYASDAMHLGDRWQVYVGLRTPYYMQGAARYRYIEPR
                     ASVLFMIDQTTSIKLAYSKMHQFVNLVQSYNATFPAEVWINSSATVKPQVSHEVTAGL
                     YKNFDANRFQTSLEVYYKKMGNQLLFGGTDTQAIDNTIEKQLIFGKGWDYGMEIFVRK
                     ATGRWTGWLAYTWAYAFQQFDSLNEGQSFPFAYDRRQMLDLSLAYSLSSHWKLGTNFL
                     IASGRAFSLSPDSSYILNPGPGQDPLYDNPGRGRGRGRGRNQGTGGSWDVVANNYRLS
                     PYNRLDFDLEYTKRRVVGSHTLETDWIFSIYNVYARPNNALVYRTIDPNTRTVVAKQL
                     PLIPVIPSLTYLLKF"
     gene            3374..4186
                     /locus_tag="VKU83_02605"
     CDS             3374..4186
                     /locus_tag="VKU83_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HLI92466.1"
                     /translation="MKLSSLLFLSGLISLPLLSCKKTASSGRNGTSHLQVMLTDGPAT
                     YDAVYIDIQQVEVNASADSNATDSGAGSGWQTVPLLRPGVYNLLDFRNGMDTVLAAAD
                     LPAGKLSQIRLVLGDSNEVVIAGQSYPMKTPSAQESGLKLNFSATLTPGIDYKLWIDF
                     NASRSIVETGNGKFILKPVLRAYTAAIGGSIKGFVLPGAAMPEVWAIQGADTMLALAD
                     TTTGYYFFGGVNAGSWNLLFQPRDTSFADSSAMVTVSTGVVTDAGTMNLRLK"
     gene            complement(4260..>4470)
                     /locus_tag="VKU83_02610"
     CDS             complement(4260..>4470)
                     /locus_tag="VKU83_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLI92467.1"
                     /translation="VTSGCGWAQREGGPVEEIHPGDVVWFSPGERHWHGASPTTAMSH
                     IAIQENLDGKVVDWMEKVTDTQYRK"
BASE COUNT          996 a         1240 c         1236 g          998 t
ORIGIN      
        1 accacgggac ttgctgtcct gggatattat cttcgcctgg cacattctgt cgaatatcag
       61 acagcggcga atgaaaccag gtcgatcagg cttgctgacg ggacgacgat gagcctggaa
      121 gaatggacaa cggttcgcgt ggaagcgggc tataacagga gcagccgggc cataacctta
      181 cttacaggca aggcccattt cgacgtgatt cacgacgccg gccggccgtt cagggtggat
      241 gccggtacgg ttgaaatccg ggatattggc acgagcttca ccgtgacact ggcgaacgat
      301 agcgtcggtg tcgaagtgat cacgggtaag gttagcgtgg aagacaaacg aacccggcaa
      361 acttttgccc tgtctgccgg acaagcggcc aataactact ctttgggcgg atctgcactt
      421 aagtttcgga atgcgccgct gtcagcggta ttggacgcct tgcaaaggcg ctatggaaaa
      481 tcaatcctcc tggccgatac caccctcgcc gcaaaaaggt taacggttaa cctggaagga
      541 gaatcatttg aagaagcaat aaaagtgatc tgcgcttcgt tggatctaat cagccatgaa
      601 gccagcaacg gaattgtcct gagcaaggcg acgcgttgat catgttactg aaccggacag
      661 ctgcatatcg gatgaaaccg cgcggcatgg cctcggccgt gttatggatg tttttgttgc
      721 tccttgcgcc gcggctttac gcgcaagact ctaccacgct tgtcactgta acgattatcg
      781 accagccctt tccggccgcg ctggccatcc tcgaaatgaa gatcccttat aagtttgctt
      841 atagcaatga gctggcgcgg atgcaaaaga acgtcagcat ccgcgcgacc gccatgcccc
      901 tccctgactt cctgcagacc ctattcagga acacggggct cagttaccgg atcatcggcg
      961 accagatcgt cgtgcagcag gcgaccaccg ccccgaggat cacgtttagc ggatatatcc
     1021 gcgatgcccg gacgggcgaa tcgctggtcg gcgccagtgt ctacgccccc ggactacgag
     1081 tgggaacact gtccaataat tacgggtttt attcgctgac cattccgcgg acggatagca
     1141 tcggattgga gatatcctat gttggttacc agccgctcga cctccaactg agtggcgccg
     1201 ccagccgaca attttccttt ccgctggagc atagcgccga acaggaggcg atcggcaagc
     1261 tggtcgtcac cagtgacagg cgcgaggaca atgtccggaa aaatcaacct gcgctggtgg
     1321 aactgtctac ggaaatggtc accgcagcgc cgtcgatcag cggaagcgga gacatcatag
     1381 gctccgtcga gatgttgccc ggggtgcagg ccgggatcga tgggaccccg gggtattttg
     1441 tccgcggcgg caatgcaggt caaaatctca ttctgctgga tgatgcgacc ttgtataacc
     1501 caagccatat cttcggactg gtcggtatct tcaacccacc ggcgatcaag tatgccagcc
     1561 tgctcaaagg cggctttccg gcttcctacg gcgaccacct tagctctgtc ctcgacgtgg
     1621 tcatgaagga cggcagcaat cagcagtatg gcggtacctt gcaggcgggc acggtctcgt
     1681 cgggggcgac actttggggg ccggtccagc cgggcaagtc atcctttctc atttcggggc
     1741 ggcgcagtac gacggacttg ctgctaaagc ccttcctgga cagcaactat ttctcgcact
     1801 actactttta tgaccttaat gccaagctga acgtcgagct tacgccgaag gaccggttgc
     1861 ttgccagttt ctatacgggc cgggataata acacctattc cagtgatacc gcctcgctcg
     1921 agggaatcaa ttatgcgatg aactatggca atacggccgt taccctgcgg tggaaccatc
     1981 aatactcgac aagattattc gggcatacca ccgtggaata taaccactat catcagttcc
     2041 tttcggctgc acaggaaggc tatttcgctc agctttattc cggcatccgg gatatggatg
     2101 ccaaatcgga actgggctgg tggctctcgc ccgctcacac catcagcggc ggcgtggact
     2161 acctgcacca gactgttttc ccttctaccc tttccggtca ggtcgctgcg gtggactcgg
     2221 ccagaaatat cgtgccgtcc ggaattcagc cgctgtcctc aaaccggttg gcgctgtacg
     2281 cgagcgacgc aatgcatctc ggcgaccgtt ggcaggtgta tgtgggcctg cgaacgccgt
     2341 attacatgca gggggcagcg cgataccgtt acatcgagcc gcgggcatcg gtgctgttca
     2401 tgatcgatca aactaccagc ataaagcttg cctacagcaa gatgcatcag ttcgtaaacc
     2461 tcgtccaaag ctataatgct acatttcccg cagaggtctg gatcaacagc agtgcgacgg
     2521 taaagcccca ggtctcccac gaggtcacag cgggcttata caagaatttc gatgcgaacc
     2581 gctttcagac cagtttggag gtttactata aaaaaatggg caaccagctg ctgttcggcg
     2641 gcacggacac gcaggcgatc gacaacacga tcgaaaagca gttgatcttc ggaaaaggat
     2701 gggattacgg catggaaata tttgtccgaa aggcgacagg gcggtggaca ggttggctgg
     2761 cgtacacgtg ggcttatgct ttccaacaat ttgactccct gaatgagggg caaagctttc
     2821 cttttgcgta tgaccggagg cagatgctgg atctctcact ggcatattcg ctttcgtcgc
     2881 attggaagtt gggaacgaac ttcctgatcg ccagcgggag ggctttttct ttgagccccg
     2941 attcttctta tattctcaat ccggggccgg gccaggatcc gctttatgat aacccgggtc
     3001 gcggacgcgg ccggggtcgt ggccgcaatc agggtaccgg cggctcctgg gacgtcgtag
     3061 ccaacaatta ccggctgagc ccttacaacc ggctcgattt cgatctggaa tatacgaaaa
     3121 ggcgggtggt gggatcccat accctcgaaa ccgactggat cttttctata tacaatgtct
     3181 atgcaaggcc aaataacgct ctggtataca gaaccattga tccaaatacg cggacggtgg
     3241 tggcaaagca gctgccactc attcctgtta ttccgagcct tacatatttg ctcaaatttt
     3301 gacccccgga ctaggaccat gggccggttt tcttgtctta tccgtgtaca gtaactctat
     3361 gaaactccca acaatgaaat tatcgtctct tctctttctt tcaggactta ttagcctacc
     3421 ccttttatcc tgtaaaaaga cggcttccag cggtcgcaat gggaccagcc atctccaggt
     3481 catgttgacg gatggtccgg cgacgtacga tgccgtatat atcgacatcc agcaagtgga
     3541 agtgaatgct tctgccgata gcaatgcaac tgattccggc gccgggtccg gctggcagac
     3601 cgtacccctg ctccggcccg gtgtttataa cctgcttgat ttccggaacg ggatggacac
     3661 ggtcctggcg gctgccgatc ttcccgcggg gaaattatcg cagattcggc tggtccttgg
     3721 cgatagtaac gaggtcgtca ttgcaggtca gtcctatccc atgaagaccc cgtcggcgca
     3781 agagtcggga ctaaaactca atttcagcgc caccctgacc ccaggaatcg attataagtt
     3841 gtggatcgac ttcaacgcca gccgatccat cgtagagacc gggaatggga aattcattct
     3901 caagccggtg ctgcgcgcgt acacggcagc tatcggcgga agtatcaaag gcttcgtcct
     3961 gcccggcgct gcaatgccgg aagtgtgggc gatccagggc gcagatacca tgctggccct
     4021 ggccgatact acgacgggat attacttttt tggcggagta aatgcgggaa gctggaacct
     4081 tttgttccaa ccgcgggata ccagttttgc agacagcagc gcaatggtaa ccgtcagcac
     4141 gggcgttgta accgatgcgg gcacgatgaa cctgcgccta aaataattgt cacctatgaa
     4201 ttccgttgga ccgggtacgg aacgcctgct ctagtgcccg ggcctttttt acccgcgcat
     4261 catttgcggt attgcgtgtc ggtcactttc tccatccaat ccaccacttt gccgtcaaga
     4321 ttctcctgga tggcaatatg cgacatggcg gtcgtcgggg aggcgccatg ccagtgtcgt
     4381 tcgcccggcg agaaccaaac cacatcgccg ggatggatct cttcgacagg gccgccttcc
     4441 cgctgggccc agccgcagcc cgaggtcacg
//
LOCUS       DATGKE010000148         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_687959, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000148 DATGKE010000000
VERSION     DATGKE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687959"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1019
                     /locus_tag="VKU83_02615"
     CDS             72..1019
                     /locus_tag="VKU83_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92468.1"
                     /translation="MIRRTALLPALILLACQGLRAQSDDDAITMAKKNLCVGAMYSHS
                     AWNYYWEGTFHRNNQNLGTVTTQMVGLMAIYGLTPRLNLVANAPYVWTDASAGTLHAM
                     HGIQDLSGWLKWEAFSRKIGAGKLKLFGAAGGSLPLTNYMADYLPLSIGLHSRTASGR
                     IIADYQLGHFFVTATGTYTWRSNITIDEASYYTTEFHQTNQVQMPNENSYIFRAGYRS
                     RSWTAEAILSGMTTLGGFDIRRNDMPFPSNRMDATMAGTHIKYEFAGGKLKGLSFTAD
                     GSYTVAGRNVGQAAMFGAGIFYTFDFSHGARGNNSLPKK"
     gene            1026..2573
                     /locus_tag="VKU83_02620"
     CDS             1026..2573
                     /locus_tag="VKU83_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLI92469.1"
                     /translation="MRINTAFRFLAGLSFITLLACNKSVSDVTASLPPLRPANQDLNA
                     GTWKPVLLSRPDTFAVAAPAAPGSPAYAADLNTIKGYQRNLTGDQLNEIKYWSAGGVL
                     RWNEIMRGLVAKYNLPPYQLANGTYPVPSSTNPFAYPLFPFSNPPYAARAYAYVSAAQ
                     YDALVACWHYKTLYNRPAPYAVDSTVKALAGKTSLPSYPSEGAVLAGVTAEMMQLLFP
                     DELASIQQKLQQAEFSLIASGSAAPSDVTAGEALGRQVADVFIARGRTDKAGTAGGNP
                     AIWASYVSAAQANGQTAWVSLETPSRPPMLPLFGQVKGFLLDSAAVVALRPGPPPSTA
                     SDSMKQLVATARYTVEHATRDQVSQVQLWADGVGTWTPAGHWNYIAAQDFVTQNWSEV
                     RWARNMALLNMAEMDAAIECWNIKYYYFNPRPSQMDPMIKTLTGIPNFPSYVSGHAMF
                     SGAAATVLGHLIPSRAQAYQQLAEQAANSRIYAGIHYAIDCEVGMSVAQQIGNYAIQR
                     AQTDGAE"
BASE COUNT          560 a          775 c          760 g          539 t
ORIGIN      
        1 gcgcgaatat cagattttaa ccatgagcat gaaagctctt caacaaagca ataaaaatga
       61 gaaccgaaac tttgatccga agaacagctc tgctgcctgc cctgatcctg ttagcttgtc
      121 aggggctgcg ggcacagtct gacgacgacg ccatcactat ggcgaaaaag aacctgtgcg
      181 tcggcgcgat gtattcgcat agcgcatgga actattattg ggagggtact tttcaccgta
      241 ataaccagaa ccttggaacg gtcacgacgc agatggtcgg gttgatggcc atatacggac
      301 tgacgccccg gctgaacctg gtcgccaatg ctccctatgt ctggacggat gcctcggcgg
      361 gcacgctcca tgccatgcat gggatccagg acctttccgg ctggctcaag tgggaagcgt
      421 tcagccggaa gatcggcgcc ggtaagctga aactgttcgg ggccgccggc ggttcgctgc
      481 cgctcaccaa ttatatggcg gattacctgc ccctttccat tgggctgcat agccggaccg
      541 cctcgggcag gatcattgcc gattaccaac tcggccactt cttcgtgacg gcgacgggca
      601 cctatacctg gcgaagcaat atcaccatcg acgaggcgtc ctattacacc acggaattcc
      661 atcagaccaa ccaggtgcag atgccgaatg agaacagtta tatcttccgg gcagggtacc
      721 ggagcagatc ctggaccgcg gaggcgatcc tttcgggaat gaccacgctc ggcggtttcg
      781 acatccggcg aaacgacatg cccttcccga gtaaccgcat ggacgccacg atggcgggaa
      841 cgcacataaa atatgaattc gccgggggta agctcaaggg gctgtccttt acggctgacg
      901 gcagctatac ggtcgcgggg agaaatgtcg gccaggctgc gatgttcggc gccgggatct
      961 tttacacgtt cgatttttcc cacggcgcgc gcggaaataa ttctttacca aaaaaataat
     1021 tcatcatgcg gatcaatact gcttttcgtt tcctggccgg cttgtcgttt atcacgctgc
     1081 tggcctgcaa caagtcggtt tctgatgtaa cggccagtct gccgcctctt cgtccggcca
     1141 accaggatct caacgcgggc acctggaagc ccgttctgct ctccaggccc gatacatttg
     1201 ccgttgcggc gccggcagcc cccggctcgc ctgcctacgc ggcggacctg aataccatca
     1261 aaggctatca gcgtaatctt acgggcgacc agctgaacga gatcaaatat tggagtgcgg
     1321 gtggcgtgct gcgctggaat gagatcatgc gggggctggt agccaaatac aatcttccgc
     1381 cctaccagct tgctaacggg acttaccctg ttccgagctc taccaacccg ttcgcatatc
     1441 cccttttccc gttttccaat ccgccctatg ccgcccgcgc ttatgcctac gtcagcgcgg
     1501 cgcaatacga tgcgctggtg gcatgctggc actataagac gctgtataac cgccctgccc
     1561 cgtatgcggt cgactcgacc gtcaaggccc tggccggcaa gacgagcctt ccgtcctatc
     1621 cctcggaagg cgccgtactg gcaggcgtaa cggcggaaat gatgcaattg ctttttccgg
     1681 atgagctcgc atcgatacag caaaagctgc aacaagcgga gttcagcctg atcgcatccg
     1741 gatccgcggc tcccagtgac gtaacagcgg gtgaagcgct ggggcgccag gtagccgacg
     1801 tttttatcgc gcgcggccgg accgataagg caggaacggc aggcggcaac ccggcgatct
     1861 gggcgtctta cgtaagtgcg gcgcaggcca acggtcaaac tgcctgggtc agcctcgaga
     1921 cgccttcgcg gccgccgatg ctgcctttgt ttggtcaggt aaaagggttt ttgctggatt
     1981 cggcggcggt ggtagccctg cgccccgggc cgcccccttc caccgccagc gattcaatga
     2041 agcagctggt ggcaacggcg cggtataccg tcgagcacgc gacccgcgac caggtaagcc
     2101 aggtgcagct ttgggcggac ggcgtcggaa cctggacgcc ggccggccat tggaattata
     2161 tcgcggcgca ggactttgtc acccagaact ggagcgaagt ccggtgggcg cggaacatgg
     2221 ccttgctgaa catggcggaa atggatgccg ccatcgaatg ctggaatatt aaatattatt
     2281 atttcaaccc ccggccgtcg cagatggatc caatgatcaa aacgcttacg ggcattccaa
     2341 atttcccgtc ttacgtgtcg ggccatgcga tgttcagcgg cgccgcggca accgtccttg
     2401 ggcatcttat cccttccagg gcccaggcct atcagcagct ggccgagcag gcggccaatt
     2461 cgcgcattta cgcgggcatt cattatgcca tcgactgcga ggtggggatg tcggttgcgc
     2521 aacaaatcgg caactatgcc attcagcggg cgcagaccga cggcgccgaa tgacaagatc
     2581 tttaggttat aagcaaaagc agaaagaaca ccgccattcc tggcggtgtt cgct
//
LOCUS       DATGKE010000149         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1126540, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000149 DATGKE010000000
VERSION     DATGKE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126540"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VKU83_02625"
     CDS             <1..199
                     /locus_tag="VKU83_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92470.1"
                     /translation="EMDGYETTQKIRREHKNNTLPIIAVTAKAMKGDRQKCIEAGASD
                     YITKPLKIDQLLSLMRVWFYK"
     gene            207..1502
                     /locus_tag="VKU83_02630"
     CDS             207..1502
                     /locus_tag="VKU83_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92471.1"
                     /translation="MQPKIMLVDDREDNLLSLETVLEPDGYRFVKATSGRQVLKLLLS
                     DFDFALILMDVQMPNINGFETASLIYERDKLRHIPIIFITANNYGEENLFKGYRAGAV
                     DYIYKPVNPELLRAKVSVFVDLYKKSQRLLAQEQKLVAINRNLELEIAERVASEQKVN
                     ELNHQLLENIARLETANKDLDLFAFMASHDLQAPLRKIRMFSDRLLASSTNGMSNESK
                     LYLTRIQEVSRRMQDLINDILRFSKISAEKQSFEEVDMNVVIGEVLSEMESVIREKHA
                     EVTVDPLPVLPASTVLMNPLFSNLISNALKYTKKNEAPRVHIRYEEAGAVSITPGKEP
                     ETRYGRIFIEDNGIGFDQKYAEQIFDMFRRLHPSSEYEGTGIGLALCKKIVEMHRGFI
                     SAIGKPGEGAVFIVSLPLHAAKTETPAHEPAGTKVKSES"
     gene            1526..>2959
                     /locus_tag="VKU83_02635"
     CDS             1526..>2959
                     /locus_tag="VKU83_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92472.1"
                     /translation="MLSHSPTRILIIEDDQDDFLIIEACIKDIPDKEFVVDWCYDYQD
                     ALQRIAHCGYQIYFVDYLLGEKTGLELLKEAKAIGCDEPLVLLTGIGDRDVDLRAMAS
                     GATDFLVKSEINKEKLERCIRYALERSANTKALKANERKFRSMFERSKDSVFLTGEDL
                     VFRDVNTATCELFKYNKEEILKISLLSLFARKEAVAQLRSRLAETGEVEDLEVELLTH
                     GKERKNCILSISREAYPSGEGYYQGIIHDITNLKRIERATFQIEKLRSTATLLRTLAH
                     EVRNPLTNINLSVEQLKPEINSEDANIYLDIIARNCGRIDSLISELLDLSRPAEISLQ
                     RAGLQEILDSTLAAASDRISLKNIRLEKAYPDKPAFVMADREKLKIALLNILINAVEA
                     VPAQSGVITVSIRKEDGRYRMYINDNGGGIPEENLSRIFEPYFTSKANGFGLGLAATW
                     NILQSHRAAIDVSSQVGEGTSFTLTFEE"
BASE COUNT          749 a          793 c          815 g          602 t
ORIGIN      
        1 ggaaatggat gggtatgaaa ccacgcagaa gatccggcgg gaacacaaaa acaacaccct
       61 gccgatcatc gccgtgacgg caaaggccat gaagggcgac cggcagaaat gcatcgaggc
      121 gggcgcttcc gattatatta caaaaccatt gaagatagat caactattgt ctctgatgag
      181 ggtatggttt tataaataga tgaatcatgc agccgaagat catgttagtt gatgatcggg
      241 aggataattt gctgtcgctg gaaacggtat tggaacccga tgggtaccgg tttgtaaaag
      301 cgacctccgg ccgtcaggtc ctgaagttgc tcctttccga ctttgacttc gcgttgatat
      361 tgatggatgt ccagatgccg aatatcaacg gtttcgagac tgcctcgctt atctacgagc
      421 gggataagct tcgccatatc ccgatcattt tcattacggc caacaactac ggcgaggaaa
      481 accttttcaa agggtatcgc gccggtgcgg tagactatat ctacaagccc gttaaccccg
      541 agctgctcag ggcgaaagtg agcgtctttg tggacttgta taagaagagt cagcggttgc
      601 tcgcgcagga acagaagctg gttgccatca accggaacct cgagctggag atcgccgaaa
      661 gggtggcctc cgaacagaaa gtgaatgagc tgaatcatca gctgctggaa aatatcgccc
      721 gcctggagac ggcgaacaag gatctcgatc ttttcgcgtt catggcctcc cacgacctgc
      781 aggccccgct gcggaagatc aggatgttca gcgaccgcct gcttgcgagc agcaccaacg
      841 gtatgagcaa cgaatcgaaa ctttacctga cacgcatcca ggaagtctcg cgccggatgc
      901 aggacctgat caacgatatc ctccggttct ccaagatctc tgccgaaaaa cagtcgtttg
      961 aagaagtgga catgaatgtc gtcatcggcg aagtgctttc agaaatggag agcgtgatcc
     1021 gggaaaagca tgccgaagtg acggtagatc cgctgccggt cttacccgcc agcaccgtcc
     1081 tcatgaatcc actattctcg aacctgatca gcaacgccct gaaatacaca aaaaaaaatg
     1141 aagccccccg ggttcatatc cggtatgaag aagcaggcgc tgtcagcatc acgcccggga
     1201 aggaacccga gacccgctac ggcaggattt ttattgagga taatggcatt gggtttgacc
     1261 aaaagtacgc cgaacagatc ttcgatatgt tccggcggct gcatccgagt tccgaatatg
     1321 aagggacggg gattgggctc gctctttgta agaagatcgt cgaaatgcac cggggtttca
     1381 tttcggccat tggaaaaccg ggggaagggg cggtgttcat cgtctcgctg ccgctccacg
     1441 cggccaaaac ggagaccccg gcgcacgagc cggcagggac caaggtgaaa tccgaaagtt
     1501 aaacacgaaa ctcgtatttt gtactatgct gagccattcg cccacacgga tcttgattat
     1561 agaggatgac caggacgatt ttttgatcat tgaagcttgc atcaaagata tcccggataa
     1621 ggagtttgtg gtcgactggt gctacgacta tcaggacgca ctgcaacgaa tagcccattg
     1681 cggctaccaa atctattttg ttgactacct tttgggggag aagacgggcc tcgagctcct
     1741 caaggaggcc aaggcaatcg ggtgcgacga accgctggtg ctgctcaccg gaatcggcga
     1801 ccgcgacgtc gacctccggg ccatggccag cggcgctacc gatttccttg tcaaatccga
     1861 gatcaataag gaaaagctcg aacggtgtat ccggtatgcg ctggagcgga gcgcgaatac
     1921 caaggcgctc aaggccaacg aacggaagtt tcgcagcatg ttcgaacggt ccaaggattc
     1981 ggtattcctg acgggcgaag acctggtgtt ccgggatgtc aatacggcca cctgcgagct
     2041 gtttaaatac aataaggaag agatcctgaa aatcagcctt ttaagccttt tcgcccgaaa
     2101 ggaggcggta gcccagctcc gcagccgcct cgccgagacc ggtgaagtcg aggacctgga
     2161 agtggaactg ctgacgcatg gcaaggagcg gaagaactgt atcctttcca tatcccggga
     2221 agcctatcca tccggcgaag ggtattatca gggcattatc cacgatatca cgaacctcaa
     2281 aaggatcgaa agggctacgt tccagatcga aaagctgcgg tccaccgcca cgctcctccg
     2341 gaccctcgcg catgaagttc ggaacccgct aaccaatatc aacctgtccg tcgagcagct
     2401 gaagcccgag atcaacagtg aagacgccaa catttacctc gatattattg caagaaattg
     2461 cggccggatc gacagtctga tctcggagct gctggatctc tcccggccgg cggagatctc
     2521 gctccagcgc gccgggctgc aggaaatttt ggactccacg ctggccgcgg cgtcggaccg
     2581 gatctccctc aagaatatcc ggctggagaa ggcttacccc gataagccgg ccttcgttat
     2641 ggcggaccgg gagaagctga agatcgccct gctgaacatc ttaatcaatg ctgtcgaagc
     2701 ggtccccgcg cagtcaggcg tcatcaccgt ctccatccgg aaggaagatg gccgctaccg
     2761 gatgtatatc aacgacaacg ggggcgggat cccggaggag aacctctccc gtattttcga
     2821 accctatttc acctccaaag ccaatggctt cggccttggc ctcgccgcga cctggaacat
     2881 cctccaatcc caccgcgcag caatcgatgt cagttcccag gtaggggagg gcacgagctt
     2941 taccctcacc tttgaagag
//
LOCUS       DATGKE010000150         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_274089, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000150 DATGKE010000000
VERSION     DATGKE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274089"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..610
                     /locus_tag="VKU83_02640"
     CDS             272..610
                     /locus_tag="VKU83_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HLI92473.1"
                     /translation="MSDIQNITNQLRKFRDERDWSQFHDSKNLAIAISIEAAELNELF
                     LWKNGEEAEEVDREKLKEELADIFSFAFLLADKHQLNVQQIILDKLRINGNKYPVDKS
                     KGTAKKYTEL"
     gene            632..2497
                     /locus_tag="VKU83_02645"
     CDS             632..2497
                     /locus_tag="VKU83_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HLI92474.1"
                     /translation="MIVYQGTKARFSEDVLTNSIDTIISSHIKKWTGKATAKSEMAAF
                     KNSLEHMDRVLQDREIPDDCGISVEYHIPQTSKRVDFIISGNDGTQDCVVIVELKQWS
                     MAELSSRDGLIRTRLQGHLVETSHPSYQAWAYAALMHSFNATIEDEAIQLRACAYLHN
                     YEPDDIITNAFYHHYLERAPAFLKPDAGKLRGFIRQYIKQGDNSDIVQRINNGKIRPS
                     KSLADSLRSIMEGNQEFIMIDDQKVVYETAMDLAIHSDEKRKNVLIVEGGPGTGKSVV
                     AINLLVDLTRRYILTHYVTKTSAPREVYFDKLQGVRKMAELRNLFVGSGSFMKTVRDS
                     IPALIVDEAHRLTKQTGFLKMGDNQVKEIIDAAYFSVFFIDEDQRVHIDDYGNVKTIE
                     RIANAAGAKVAKMTLSSQFRCNGSDGYLAWLDEILQIRETANEEFSSDFEFDFKVVDS
                     PAILRDMIFEKNKVSNKARLVAGYCWDWNSDKNPDAYDICFPEYDFKMRWNLKAYGST
                     WIIDPNSVQEVGCIHTCQGLEVDYIGVIVGEDLIFRDGQVLVDPGKRSKMDKSISGYK
                     RRMKEKPEETRKLVRTIIKNTYRTLMTRGMKGCYLYCTDKQLDGYIKDRLAIAGV"
     gene            2539..3807
                     /locus_tag="VKU83_02650"
     CDS             2539..3807
                     /locus_tag="VKU83_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HLI92475.1"
                     /translation="MLTKTVTAICLAFSPMLAIAQVPPGSIALYPLNNSANDVSGNGY
                     NGTLTSTTSATNRFGTAGSATGFTAGTSTGTLPNNLVVAMQDDFSIGYWFNSTMTAPS
                     SSQWYGGAALVDAEVCGGTSDWGTALINGGSVAFGIGNPDITVISPGTTYNDGNWHFV
                     VATRAESAGTITLYIDGAQVATTSGTATTARTAPSIIGLGRNNCVATGVYTGSLDDAI
                     AYARVLSSTEVSNLYNYYSGIALPLTWIGFTGEAEGGAVLLRWQTADAAGNDHFEIDR
                     STDGSHFSDIGMLPNADDMVTGPGVQSYQFTDPNPLQGNNFYRIKQIDADGRYSYSRV
                     VEVNVGRVASGFQLQTNPARSEVTLLNAGQQTVEMLQVLDVSGRAIINAAPNSSHSLI
                     NVDVSGLRPGYYLLRVGTRDGVVSMPFMKY"
     gene            complement(3841..>4027)
                     /locus_tag="VKU83_02655"
     CDS             complement(3841..>4027)
                     /locus_tag="VKU83_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92476.1"
                     /translation="ERTQSLESRTAVLDSVVRQQAHLIVELKRLNSTAIRTIVRLQRN
                     FDQYQERERSAAQTAEQ"
BASE COUNT         1041 a          940 c         1107 g          939 t
ORIGIN      
        1 agttcaaacg gtggaaggaa ggttggcagt gagagttgtg gtttgtctat gcaacattta
       61 agcccagggc agcgtcaggc tgaagcagta tggtcaggag attggggatt caccaaacaa
      121 aaagcgtgtc acgcctctaa aaaatttcct accttgttcg tggttccggg taatcgattg
      181 gacccgggga agcgggaagc tggatgctcg attttcggga tctcgttttc agaaagtcat
      241 ttgagatggc tacattcttt aatttctgat tatgtccgat attcaaaata ttacaaacca
      301 attacgaaag ttccgggacg agcgtgactg gtcccagttc catgattcca agaatctcgc
      361 cattgccatt tcgattgagg cggcagaatt gaacgagcta ttcctttgga agaatggcga
      421 ggaagccgag gaagtcgaca gggaaaagct aaaggaggag ttggccgaca ttttctcctt
      481 tgccttcctt ctggcggata agcatcagtt aaacgtgcag cagatcatcc tcgataaact
      541 ccggatcaac ggtaataaat atcccgttga caaatcgaag ggcactgcaa aaaaatacac
      601 tgagctctaa acgaagaaat cctaacctct aatgattgta taccagggca ctaaagcccg
      661 ttttagtgag gatgtgctga caaacagcat tgacacgatc atcagttctc atattaagaa
      721 atggacagga aaggcaacag cgaagagtga gatggctgca tttaagaact cgctggagca
      781 tatggacaga gtgctgcagg accgcgaaat tccagacgat tgcggtattt cggttgaata
      841 tcatattcca cagacctcaa aaagggtcga tttcatcatt tccggcaacg atggcacgca
      901 agactgcgtg gttattgtag aattgaagca atggtcaatg gccgaattgt cgtcccggga
      961 tggccttatc agaaccaggt tgcaaggcca ccttgttgaa accagccacc cgtcatacca
     1021 ggcctgggca tatgcggcac tcatgcattc cttcaatgcg acgattgagg atgaagccat
     1081 ccaattgcgc gcctgcgcct accttcataa ttatgaacct gatgacataa taactaatgc
     1141 attttaccac cactaccttg aaagggctcc agcctttctg aagcctgatg caggcaagct
     1201 gcgcggattt atcaggcaat atataaagca gggggacaat tcggatattg tacagcggat
     1261 caacaacggt aagatccgcc cttccaaaag cctggcagat tccctgcggt cgatcatgga
     1321 aggaaaccag gaattcatca tgatcgatga ccagaaggtg gtgtacgaaa cagccatgga
     1381 tctggccata cattccgatg agaagcgcaa gaacgtcctg attgtcgagg gcggccctgg
     1441 aacagggaag tcggtcgtcg ccatcaatct gctggtggat ttgacaagga gatacatttt
     1501 gacccactat gttaccaaga ctagtgcgcc ccgggaggtg tattttgaca agctacaggg
     1561 agtaagaaag atggcggagc tgaggaacct ttttgttggt tcgggcagct ttatgaagac
     1621 ggtgcgagat agcattccgg cgctgattgt cgatgaagcg catcgactga ctaaacaaac
     1681 cgggttctta aaaatgggtg acaaccaggt aaaagaaatc atcgatgcgg catacttttc
     1741 ggtgtttttt atcgacgagg accaacgggt gcacattgac gactacggta atgtcaagac
     1801 tatagaaagg atcgctaacg cggcaggggc caaggtcgcc aaaatgacat tatcctccca
     1861 gttccggtgc aatggatcgg atggctacct ggcctggctg gatgaaatct tacagattcg
     1921 ggagaccgcc aatgaagaat tttcttcaga ttttgaattc gatttcaagg tggtcgacag
     1981 cccggctatt ctcagggata tgatatttga gaagaataaa gtcagtaata aggcgaggtt
     2041 ggtggcaggg tattgttggg attggaatag cgataagaac ccagacgcct atgatatttg
     2101 ttttcctgaa tatgatttta agatgcgctg gaacctgaag gcttatgggt cgacctggat
     2161 catcgatccg aattcagttc aggaggtggg atgcattcat acctgccagg gcttggaagt
     2221 ggactatatt ggggtgatcg tcggggagga tttgattttt agggacggtc aggtgttggt
     2281 ggatcccggt aaaaggtcga aaatggacaa gtctatttcg gggtataagc ggaggatgaa
     2341 agaaaaaccg gaggagacga ggaaacttgt aaggacgatc attaagaata cgtatcggac
     2401 gctgatgacc agagggatga aggggtgtta tctgtattgc acggataaac agttggatgg
     2461 ctatataaag gatcgattgg caatagcagg cgtttgacac gactaagaca attcatcgtt
     2521 aactaaaaca attgcatcat gcttaccaaa actgtcactg cgatttgtct cgccttctct
     2581 cccatgcttg ctatagcgca agttccgccc ggaagcattg cgctttatcc actgaataat
     2641 tcggccaatg atgtgagcgg caacggatat aacggaaccc tgacgtccac cacgagcgca
     2701 accaatcgtt ttggtaccgc cggcagcgcc accggattta cggcgggtac ctccaccggg
     2761 acccttccca acaaccttgt cgtggccatg caggacgact tcagcatcgg atattggttc
     2821 aacagcacca tgaccgcccc cagctcgtcg caatggtatg gcggtgcagc attggtcgat
     2881 gcagaggtat gcggcgggac aagtgactgg ggaacggcat tgatcaatgg aggctccgtt
     2941 gcgttcggga tcggcaaccc ggatatcacg gttatctcgc cggggacaac ctataatgac
     3001 ggcaactggc atttcgttgt agccacacgt gccgagtcgg ccggtacgat tacactctat
     3061 attgatgggg cacaggtagc cacgacgtcc ggcaccgcaa cgaccgcacg tacggcgcca
     3121 tctatcatcg gcctgggccg gaacaattgt gttgctaccg gcgtctacac cggctccctc
     3181 gatgacgcca ttgcgtatgc gcgcgtgttg tcttctaccg aagtaagtaa cctgtataat
     3241 tattacagtg ggattgcttt accgctgacc tggattggat ttaccggcga ggcggaagga
     3301 ggggccgtcc tcttacgttg gcaaacggca gacgctgccg gcaacgatca cttcgagatt
     3361 gaccggtcaa cagatggcag ccattttagt gacatcggca tgctcccgaa tgcggatgac
     3421 atggttaccg gtcccggagt ccagtcctac cagttcaccg acccaaaccc gctacaaggc
     3481 aataatttct accggatcaa gcagatcgat gcggacggcc gctattcgta ttcgcgcgtc
     3541 gtcgaggtca acgttgggag ggtggcctcc ggattccagt tgcaaaccaa cccggcgcgg
     3601 agcgaggtga ccctgttgaa tgccgggcag caaacggtgg aaatgttgca ggttttagac
     3661 gtatccggca gggccatcat caacgccgcg cccaattcga gccactcgct gataaatgta
     3721 gacgtgtcgg gcctgcgacc ggggtattat ctgctccggg tcgggaccag ggatggagtg
     3781 gtgagcatgc cgtttatgaa gtattaggcg cacttggccc tggaccaagc tggaaaatag
     3841 ttactgttcc gctgtctgcg ccgcggaccg ctccctttcc tggtattggt caaagttccg
     3901 ctgcagtcgg acaatggtac ggatagcggt cgaattcagc ctctttagct ctactatgag
     3961 atgggcctgc tgcctcacga cggaatccag gacggcggtc ctcgactcca ggctctgcgt
     4021 ccgctcc
//
LOCUS       DATGKE010000151         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_896410, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000151 DATGKE010000000
VERSION     DATGKE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896410"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKU83_02660"
     CDS             complement(<1..207)
                     /locus_tag="VKU83_02660"
                     /inference="COORDINATES: protein motif:HMM:NF044433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92477.1"
                     /translation="MLRGVAQTATEAIHQIKEINELFSATKEKIKKQAEKSYNKELLE
                     LLFEHPYSKIDHVIDRLQVSRVTAA"
     gene            complement(312..626)
                     /locus_tag="VKU83_02665"
     CDS             complement(312..626)
                     /locus_tag="VKU83_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92478.1"
                     /translation="MLKSYFQMAWRNLQKHKASAAINIGGLTVGLTVSLLVVLFVIDE
                     FGFDRFHRNIAELYLLMKNQQQADGVSTNRTSAGPIAGALRATLPEVANAARVAGTAN
                     KG"
     gene            1257..>2880
                     /locus_tag="VKU83_02670"
     CDS             1257..>2880
                     /locus_tag="VKU83_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92479.1"
                     /translation="MILNASNSWHPLWPRIAMFRNYFKIAWRNLRKNKVFSFINIAGL
                     SLGISVCFIITLYVRDELCYERYNVYADRIARIQFRAVMGGSQISEAGVMAPVAKTLR
                     SDYPEVENATRLLPLGKSKVVCERREFRNDLLALVDSNFFDIFTLPLSRGDARTALAQ
                     PHSVVISQAMAEKYFGKQDPVGRTLAIDDDQTLYKVTGVFAGMPSHSDFRFDMLGSLA
                     GFQPAQSDSWLDGSYRTYLLLKPRTDLAALQAKFPEMVKKYMGPQIQRVMHMSLDQFM
                     SKGNQLGFVLQPLTAIHLHPIATTNDASATDGFAPPGDASYVYIFGAIAGFMLLIACI
                     NFVNLSTAGASKRAREVGVRKAIGSDRSQLIAQFLTESAVVVFIALALSSAIIYFSLP
                     VFNAISGKALSFTLSPGIFAMLALFGLIVIVAAGMYPAFFMSAFKPARVLKGKLTANH
                     SSFRLRSGLVVFQFVISVSLIIGTIVVYGQMRYIQNKELGYVKEQVITIPNSAALMKN
                     EALFRDIMLKDPRVISATMSQYKPAGASNSNNS"
BASE COUNT          630 a          759 c          754 g          737 t
ORIGIN      
        1 ggcggcagtc acgcgggaaa cctggagacg atcgatgacg tgatcgattt tgctgtatgg
       61 atgctcgaac aacagctcca gtaattcttt gttataagat ttttccgctt gcttcttaat
      121 tttctccttc gttgccgaaa aaagctcatt tatctccttg atttggtgga ttgcttcagt
      181 tgccgtttgg gcaacccccc gcaacataaa taaaatccat tcctcccaat tgtttttgag
      241 ccgtacatcc tgcagcagcc gataataaag aggcagtcac caattggcgt aaggtattga
      301 tagtctctat cttatccttt gttggcggtg ccggccaccc gtgcggcatt cgctacttcc
      361 ggaagagtgg cccgcaatgc gccggcaatc ggtcccgcgc tggtccggtt cgtggagacg
      421 ccatctgcct gctgctggtt tttcatcaga agatacagtt ccgcgatatt ccggtgaaac
      481 cggtcgaatc caaattcatc tattacaaat aatacaacca gtagacttac cgtgaggccc
      541 acggtcaggc cgccgatatt gatagccgca gaggccttgt gtttttgcag attccgccag
      601 gccatttgga agtaactctt aagcatatgg aatagacttt gaatggatca gtttctggat
      661 gagacaggta accaagacga tgccaactcg caacttgttt gtaaatcaac aatttaattt
      721 ttgcggaagc ggcgcaggtg cccgatttcg atgcaactgt gtttgatttt gattgaagaa
      781 aaccggcaga ctgaggcgcc ggctttcacc gggctttaac acttgcctat ccaggattgg
      841 ctaactttgt gttaggattg gttagtttat ggccaataag ctaatatatt ggttacccat
      901 cgttggggtg tttgttagtt tggtccatta cgataaagac aatggcatga gcgcgttctg
      961 gtgttattat cagaccgccg cgctcctggt cacgatctgg gttctggcct ttatctttta
     1021 cggaaatccg taagcggtcc gcgaacaaac gctcctcctg acggctttac atcccgccct
     1081 attttccggg aagggccgcc gcatagtctt caggcgaggt ttttgatccg gcaatgtccc
     1141 ggcagccctc cggcagccct ccggcgccct ccggcagtct cccggcagcg ttccgaaagc
     1201 gagcagcgat tgtatcgaaa gcgcacggta gctttgccaa aatcttcctt agtcgtttga
     1261 tactcaacgc ttcaaattcc tggcatcccc tttggccgcg catcgccatg tttcgcaatt
     1321 acttcaagat cgcctggcgc aacctccgga agaataaggt cttctccttt atcaatattg
     1381 cgggcctttc cctgggcatt tcggtgtgtt ttatcatcac cctttatgtc agggatgagt
     1441 tatgctacga gcggtacaat gtatatgcgg accggatcgc ccggatccaa tttagggccg
     1501 tcatgggcgg gagccagatc agcgaggcag gagtaatggc gccggtggcc aaaacgctca
     1561 ggtcagacta tcccgaagtg gaaaatgcaa cccggttgct gcccttgggg aagtcaaagg
     1621 tggtctgcga aagaagagaa ttccggaacg acctgctggc cctggtggac tcgaattttt
     1681 ttgacatttt caccttgccg ctgagccggg gagatgcgag gaccgcgctg gcgcagcccc
     1741 attccgtcgt gatatcacaa gccatggcgg agaagtattt cggaaagcag gatccggtcg
     1801 gcaggacgct tgcgatcgac gatgaccaaa ccctgtataa agtgacgggc gtcttcgccg
     1861 ggatgccttc ccattcggat tttcgtttcg acatgctggg ctcgctggcc ggctttcagc
     1921 ctgcgcaatc cgactcatgg ctggatggga gttaccggac atacctgttg ctcaaaccgc
     1981 gcaccgacct tgccgcgctg caggcgaaat ttcccgaaat ggtgaaaaag tacatgggcc
     2041 cgcagattca gcgggtcatg cacatgagcc tcgatcaatt catgagcaag gggaaccagc
     2101 tcggctttgt tttacagcca ttgacggcta tccatctgca ccccatcgcg accacaaacg
     2161 atgccagcgc gacggatggg ttcgcccctc ccggtgacgc cagctacgtg tacatttttg
     2221 gcgccatcgc aggattcatg ctcctcatcg cctgtattaa ttttgtcaat ctttccaccg
     2281 ccggggcatc aaaacgggcc agggaggtag gggtgcgaaa ggcgatcggg tcggaccggt
     2341 cccaattgat cgcccaattc ttaaccgaat ccgccgtggt ggtattcatc gccctggcgc
     2401 tatcctccgc gatcatttac ttttctttgc cggtattcaa tgcgatttca ggcaaggcgc
     2461 tgtcgtttac actcagtccg ggaattttcg ccatgctggc cttatttggc ctgatcgtga
     2521 tcgtagcggc aggcatgtac cccgcgtttt ttatgtccgc tttcaagccg gcgcgggttt
     2581 taaagggcaa actgacggcg aatcattcca gtttcaggct ccggagcggt ctggtcgtat
     2641 ttcagttcgt catttctgtg tccctgatca tcggaaccat cgtggtctat gggcagatgc
     2701 ggtatatcca gaacaaggag ctcggttacg tgaaggaaca agtgattacg attcccaatt
     2761 cggctgcgct gatgaaaaat gaagccctat tcagggatat tatgctgaag gacccgagag
     2821 tgatcagcgc taccatgtca cagtacaaac cggccggcgc cagcaacagc aacaacagcc
//
LOCUS       DATGKE010000152         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_66884, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000152 DATGKE010000000
VERSION     DATGKE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66884"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VKU83_02675"
     CDS             <1..463
                     /locus_tag="VKU83_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92480.1"
                     /translation="KHLRLVISVPEAYTGYLSDKEKVNFTVKALPNRNFTAQVRRLAG
                     ALDNHLRAERTEMDVYSDDKTLLPGMVAEVELPLPARDSTFIVPGSAVVNGTERLFVI
                     RETADHRAEWVDVQTGRSQGGKIEIYGKGLNEGDRLVRTGSEEIRDGAKIP"
     gene            complement(517..>2110)
                     /locus_tag="VKU83_02680"
     CDS             complement(517..>2110)
                     /locus_tag="VKU83_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HLI92481.1"
                     /translation="ALLIFILSFIFIHSHAQQAEPRLYSANSLPSATISKVASFAPNR
                     PFPQHNSYQEGTILPDHVSQEEMDDSVKSFYQAWKKHYILPACREGESYVWFEGSHGS
                     NICVSEGQGYGMVIVALMAGFDSSAQETFDRLYRFYKSHPSTTSPHLMAWTQKKDCIS
                     LDGGAATDGDMDIAYSLLLADAQWGRHSNIPYRQEALAMIDAIRHQEINPYTYIVMED
                     NTGRPRSKDYYDMRSSDFMPDHLRAFHEATGDPFWDTVITNNYRVFRYLQNTYSPEAG
                     LVPDFIKNIHISQDRLGAGPGQVATEGKKNDTSFVTGAGVRTANSLNPRARSAGSTPP
                     NAESSDYPGTGPFADGDPDDAHLFAQPVQPNYLESKFDGVYNFNACRVPWHVATDFLV
                     SGDPRAAAFLTRINKWIQATTKGNPDNISAGYNLSGEDLPHRYFEALCFICPFAVAAM
                     SSADNQEWLNRLWDYIQHFKMKNFDYYDNTIKMLTMIILSGNYWTPATSPGTFLASHS
                     QPSSPVHQRQLPGSAAQDRSAR"
BASE COUNT          471 a          493 c          675 g          471 t
ORIGIN      
        1 aaaacatctg cgattggtaa tttctgtgcc agaggcctat acgggttatc tcagtgataa
       61 ggaaaaggtg aactttacgg tgaaggccct cccgaacagg aacttcaccg cgcaggtacg
      121 gcggctcgcg ggagcgctgg acaatcattt acgggccgaa cggacggaga tggacgtcta
      181 tagcgatgat aaaaccttgc tgccaggaat ggtggccgag gtggagctgc cgctgccggc
      241 gagggacagt acgtttatcg tgccgggttc ggcggtcgtg aatggtacgg aaagattgtt
      301 tgtgatccgg gagacagccg atcaccgggc cgaatgggtg gatgtgcaga caggtaggtc
      361 acaaggcgga aagatcgaaa tttatggcaa gggcctgaat gagggcgacc gtttggtcag
      421 gaccggcagc gaagagatca gggatggggc gaagatcccg tgaggggatt agagcgcgct
      481 ctcgcggcgt tccagcggcg ctccgcggaa cggtggttat cgggcggaac ggtcctgggc
      541 tgcagagccg ggcagttgcc gctgatgcac ggggcttgag ggctgggaat ggcttgccag
      601 gaaagtgccg ggggatgtgg ctggcgtcca gtagttgccc gatagaatga tcatggtcag
      661 catcttaatg gtgttatcgt aataatcgaa attcttcatc ttgaagtgtt ggatgtaatc
      721 ccacaatctg tttagccatt cctggttgtc ggcgctggac atggcggcaa cggcgaacgg
      781 acagatgaag cagagtgctt caaaatagcg gtgcggcagg tcttcgcccg agagattata
      841 gccggcagag atgttatcgg gattaccctt tgtcgtcgcc tggatccact tattaattcg
      901 ggtcaggaac gcagcggcgc gagggtcgcc ggaaaccagg aagtctgtgg cgacatgcca
      961 tggtacccgg caagcgttga aattgtacac gccgtcgaat ttcgattcca ggtaattggg
     1021 ctgaaccggt tgggcaaaga gatgggcatc atcggggtcg ccatcagcga agggaccggt
     1081 accaggataa tcggaagatt cggcattcgg gggagtggat cccgctgatc tggcacgggg
     1141 attcagcgag ttggcggtgc ggacgccggc gccggtaacg aaggaggtat cgttcttttt
     1201 cccttccgtt gcaacctggc ccggaccagc gcccaggcgg tcctgtgaga tatgaatatt
     1261 cttgatgaag tcgggtacca atccggcttc ggggctgtaa gtattctgca aatatctgaa
     1321 gacgcggtaa ttgttagtga tcaccgtgtc ccaaaaggga tcgccggtgg cttcatggaa
     1381 agcgcgcaaa tggtcgggca tgaaatccga cgaccgcata tcataataat ccttgctgcg
     1441 cggtctgccc gtattgtctt ccataacgat gtaagtgtat ggattaatct cctggtggcg
     1501 gatggcgtcg atcatagcaa gcgcttcctg tctgtaagga atattgctgt ggcgtcccca
     1561 ttgcgcgtcg gccagcagca gggaataggc gatatccata tcgccgtctg tggcggcgcc
     1621 tccgtcgagg ctgatgcagt ccttcttttg tgtccacgcc atcaggtgcg ggctggtcgt
     1681 actcggatgg gatttataga agcgatacag gcggtcgaaa gtctcctggg cgctggaatc
     1741 aaatcccgcc attagcgcca cgatgaccat gccgtagccc tgtccttccg acacgcaaat
     1801 attggatccg tggctgcctt cgaaccagac ataactttct ccttcgcggc aggcagggag
     1861 gatataatgc ttcttccacg cctgataaaa tgacttgacg ctgtcatcca tttcttcctg
     1921 gctcacgtgg tcggggagta tagtaccttc ctgatatgaa ttgtgctggg ggaagggccg
     1981 gttaggagcg aagcttgcga ccttcgaaat cgtcgccgac ggaagactgt tggcggaata
     2041 aagccgcggt tcggcctgct gtgcatgtga atgaataaaa ataaaggaca ggataaagat
     2101 taatagagcc
//
LOCUS       DATGKE010000153         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_89381, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000153 DATGKE010000000
VERSION     DATGKE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89381"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1168)
                     /locus_tag="VKU83_02685"
     CDS             complement(179..1168)
                     /locus_tag="VKU83_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLI92482.1"
                     /translation="MAKAGSTSLSYESLAIGIDIGGTGTKYGIVDRNGNVLFSGEMST
                     RGHKDLDAFIAQLHANLLKLIEKAGGSGRMRGIGVGAPNGNFYSGTVEYAPNLPWKGI
                     LPLAKAIQNQFQLPAVLTNDANAAAIGEMMYGAAKGMKDFIMITLGTGVGSGIVANGK
                     LIYGHDGFAGELGHTIIVPNGRYHEGTGKKGSLESYASATGVMLTAIEMLEKSNEDSL
                     LRKVPKEKLDSRKVYDAAIQGDKLAKEVFDFTGSILGLALANFVMFSSPEAIILFGGL
                     TKAGDLILKPTRENMEANLIQVFQNKVKILVSHLKESDAAILGASALVWDMKG"
     gene            complement(1211..2821)
                     /locus_tag="VKU83_02690"
     CDS             complement(1211..2821)
                     /locus_tag="VKU83_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI92483.1"
                     /translation="MSQSIQTKQPTNTGALFTLVLVFFFWGFIAASQSIFIPFCKHNF
                     SLTQFESQLLGSAFYGAYFIGSLALFLISSGLKYDILNRIGYKKGIIYGIWISVAGAL
                     LIIPFANAHSFAGLLTAYFVIALGFSLQQTAAQPFAIILGDPSTGSHRLNLAGGVNSF
                     GTTLGPIIFSVFLFGKVGGDASAATVTNINNLFLIVACVFAAVALFFTFSPLPSGKDD
                     DHFEASYKASGSLILMTLLIGVIIVIGQLTNIQKLPLLIVTLVVVLGILFSSNGMAVK
                     NPSHWGAMKYPQLIYGMLGIFLYVGVEVTIDNNFGALLRVPHYLTDAGLDESKISKYV
                     SLYWASLMIGRWTGAIGVFNLTETGKRIATIVVPFVAFGVALLVNHLYSGDISDLYPY
                     AAFVAIAIIAFFYGQQKPVKTLLTLALLAGAFILTGVLTSGHLSVYCIMAAGLCNSIM
                     WPCIFSLGVAGLGKYTSQGSAFLIMMILGGAVVPPFQGAVGDLAGMHNSYLVAVACYL
                     VLAWLALKLRSVLKSQGLNFDEQIGAGH"
BASE COUNT          749 a          871 c          802 g          667 t
ORIGIN      
        1 tggcttacct gattatggcc cgctggctcg aatcctacgc gtaccggctc cctgtgtctc
       61 cagccccttt cctgatcgcc ggcctgagca cgctgttcct gctcctcgcg ctcattttca
      121 ctcaatgtta ccgggtatcg gtcgcaaacc ccgtgggcag tctacgatcc gactagcctc
      181 agcctttcat gtcccacacc agcgcactcg cgcctaatat tgctgcatcc gactctttga
      241 ggtggcttac gaggatcttc accttgtttt gaaacacctg gatcagattt gcctccatat
      301 tctcccgggt cggctttagg atcaggtccc ccgccttcgt caggccgccg aaaaggatga
      361 tcgcctccgg gctcgagaac atcacaaaat tggccaacgc caaaccaagt atcgaaccgg
      421 taaaatcaaa gacctccttc gcaagcttgt cgccctgtat tgccgcgtca tagaccttcc
      481 gcgagtccag cttctccttc ggcaccttcc gcagcaaact gtcttcgtta cttttttcga
      541 gcatctcgat cgcggtcagc attaccccgg tagcggaagc atagctttcg agcgatccct
      601 tttttcccgt tccctcgtga taccggccat tggggacgat aatggtatgg ccgagctcgc
      661 cggcaaaccc atcgtggccg tagatcaact tgccgttcgc gactatgccg ctgcccacgc
      721 ccgtacccag cgtgatcatg ataaagtcct tcattccctt ggccgcgcca tacatcattt
      781 cgccaatcgc cgccgcgttg gcgtcattgg tcagcaccgc cggaagctga aactgattct
      841 ggatggcctt tgcaagcgga agaatgccct tccacggaag gttgggtgcg tattcgacgg
      901 tgccgctgta aaaattgcca ttcggcgcgc caacgccgat gccgcgcatc cgccccgaac
      961 ccccggcctt ctcgatcaat ttgaggaggt tcgcatgcaa ctgcgctata aatgcgtcca
     1021 ggtccttatg cccccgcgtc gacatctcgc cggaaaacag gacgtttcca ttccggtcaa
     1081 cgatcccata tttggtgcca gtccccccaa tgtcgatgcc gattgccaat gattcatacg
     1141 ataacgaagt agatccggcc tttgccatga gatatccttt tataaatgat ttcgacaata
     1201 agttgtgtcc tcaatggccg gccccgatct gttcgtcgaa gttcaatccc tgcgacttga
     1261 gcacgctccg gagcttcagc gccagccatg caagcacgag atagcaggcc accgccacca
     1321 ggtagctgtt gtgcattccc gccagatctc cgaccgcccc ctggaaaggc gggacgacgg
     1381 cgccgcccag gatcatcatg atgaggaacg ccgagccttg tgaagtatat ttcccaagcc
     1441 ccgcgacacc cagcgaaaag atacacggcc acatgatgga attgcaaaga cccgccgcca
     1501 tgatgcaata gaccgaaaga tggcccgatg tcaggacgcc ggtcaggata aaggcgccgg
     1561 ccagcagggc gagggtcaac agcgtcttca ccggtttctg ctgtccgtaa aaaaaggcga
     1621 tgatagcaat agccacaaat gcggcgtaag gatacagatc cgaaatgtcg ccgctataaa
     1681 gatgattgac gagcagcgca acaccgaagg caacaaaagg caccacgatc gtcgcgatcc
     1741 gcttaccggt ttcagtcaga ttgaatacac cgatcgcccc cgtccagcgg ccgatcatca
     1801 ggcttgccca gtagaggctt acgtatttgg agatcttgct ttcgtcgagg cccgcatccg
     1861 tcaggtaatg cggcacgcgc agcaatgccc cgaaattatt gtcgatggtt acttctacgc
     1921 ctacatacag gaatatcccg agcatcccgt agataagctg cggatacttc atcgcgcccc
     1981 agtggctcgg gtttttaacg gccatgccgt tggaggaaaa aaggatcccc aacacaacga
     2041 ccagggttac gatcaggagc ggtagtttct ggatattggt cagttgtcca atcacgatga
     2101 ttaccccgat cagcagcgtc atcaggatca gtgaaccgga agccttgtat gaagcttcga
     2161 aatggtcatc atcctttccc gagggaagcg gggaaaaagt gaagaacagc gctaccgctg
     2221 caaaaacgca agccacgatc aggaacaggt tattgatatt ggtgacggtt gccgcgctgg
     2281 catcgccacc gaccttaccg aacaggaaca cgctgaagat gatcgggccg agcgtcgtcc
     2341 caaaagaatt gacgccgccg gcaaggttca gccggtggct gcccgtcgaa gggtcgccaa
     2401 ggatgatcgc gaagggctgg gcggcagtct gctggagcga gaagccgagc gcgataacaa
     2461 agtaggcggt cagcagcccg gcgaaagaat gggcgttggc gaagggaatg atcagcaagg
     2521 cgccggctac ggagatccaa atgccgtata tgatcccttt cttatagccg atccggttca
     2581 ggatatcata tttaagtccg cttgatatga gaaaaagcgc aagcgatccg atgaagtatg
     2641 caccatagaa cgcggagccg agcaactgcg actcgaattg agtcaaactg aaattgtgct
     2701 tacagaaggg gataaaaata ctttgcgaag cagcaatgaa tccccagaaa aagaagacga
     2761 gaacgagcgt aaagagtgca ccggtgttgg tgggttgttt tgtttggatg ctttgactca
     2821 cggtgttgga attagggtct gaaaatagcg ttatttcccg aagcgaaaaa ttttttcatc
     2881 cccggtacgt cttataataa aaaaccttgc taaattgaat gacaatagct tacagctcaa
     2941 atggaaatac ttcacgtatc ggcggaatgt tatcccgtgg caaaggcagg tggattgggc
     3001 gacgtcgtgg gggcattgcc aaagtatcag cgcgacctcg gccatatcgc caaggtcgtc
     3061 atgccgatgt ataaaaccaa attcctggc
//
LOCUS       DATGKE010000154         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1415263, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000154 DATGKE010000000
VERSION     DATGKE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415263"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VKU83_02695"
     CDS             complement(<1..212)
                     /locus_tag="VKU83_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92484.1"
                     /translation="MKVNKHFSTLLFFWTSGWALLAVFGAGCGAPARNAEKKVFYYNE
                     SDGVATLDPAFAKNRSIMWVIHQLYST"
     gene            245..>2025
                     /locus_tag="VKU83_02700"
     CDS             245..>2025
                     /locus_tag="VKU83_02700"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI92485.1"
                     /translation="MSRKLLLLAGFSFLSGLTSQAQLLTPQRTFTRADTLRGSITPER
                     AWWNLLKYDLQVTPDYNSQTLAGENAITFQAIAPGQMMQIDLQEPMNLLSATWGKKKL
                     SYSREGNVFHLRWPRELKAGDIETVHLKFEGKPRVAVRPPWDGGWIWTKDKEGRPWMT
                     VADEGLGASCWFPCKDHLYDEPDSGVVLSITAPDSLVAIGNGRLREKKDNGNGSTTWT
                     WAVLNPINSYDIIPYIGKYVNWSEEYGGLKGRLDCSFWVLDYNLDKAKPQFKQVDTML
                     DAFEYWMGPYPFYEDGYKLVEAPHLGMEHQSNVAYGNHFENGYLGHDLSGTGWGLKWD
                     FIIVHESGHEWFGNSITDKDAADMWVHEGFTNYSETLFTTYMYGVEAGNDYVIGTRKN
                     ISNESTIIGTYNVNKEGSGDMYYKSGNMLHMIRQIMGDSAFRGMLHGLQKTFYHQTVT
                     TQQIEDYCSQYAGKDFSKIFDQYLRTTQVPVLTYQSSGDVISYRWVNCVKGFNMPVKV
                     YCGTQPEKWIYPTEDWQTMPAGSSGQILRGAGGQMPPQTRAGQAGHRSPQGPGPKIEI
                     VGQTGNGSGGDGNLSVDRNFYIIMRKAG"
BASE COUNT          498 a          570 c          569 g          388 t
ORIGIN      
        1 gtactgtata gctggtggat cacccacatg atggaccggt tcttcgcgaa ggccggatcg
       61 agcgttgcga caccgtccga ttcgttgtaa taaaatactt ttttttccgc attcctcgcc
      121 ggagcgccgc aacccgcacc gaagaccgcc agcaacgccc aaccgcttgt ccaaaagaaa
      181 aggagggttg aaaaatgttt gtttaccttc attccggata ccaacgttaa attaaagata
      241 cccaatgagt cgtaaactgc tgctgcttgc gggattttct ttcctttccg gcctgaccag
      301 ccaggcccag ctcctgacac ctcaaagaac gtttacccgc gccgacaccc tgagaggaag
      361 catcactccc gaacgcgcct ggtggaacct gttgaaatac gacctccagg taacgcccga
      421 ttacaacagc cagacgctcg ccggcgagaa tgccatcacc tttcaggcca tcgcgccggg
      481 gcagatgatg cagatcgacc tgcaggaacc gatgaacctt ttgtccgcaa cctggggaaa
      541 aaagaagctc agttattcgc gcgagggaaa tgtctttcat cttcgctggc cgcgggagct
      601 gaaggccggc gatatcgaaa ccgtccatct gaaattcgaa ggcaagccgc gcgtggccgt
      661 gcggccgccg tgggatggcg gctggatctg gacgaaggat aaggagggac gcccctggat
      721 gaccgtggcg gatgagggac ttggcgccag ctgctggttc ccctgcaaag accatctgta
      781 tgacgaaccg gatagcgggg tcgtcctgag catcaccgcc cccgacagcc tggtagccat
      841 tggcaacggc cggctccggg agaaaaaaga taacgggaac ggctccacga cctggacctg
      901 ggctgtactc aaccccatca attcttacga catcattcca tatatcggga aatacgtcaa
      961 ctggtcggaa gagtatggag gactgaaagg caggctggat tgcagtttct gggtgctcga
     1021 ctataacctc gacaaggcaa agccgcaatt caagcaggtc gataccatgc tggatgcctt
     1081 cgaatactgg atgggcccct atccctttta tgaggacggt tataagctgg tcgaagcccc
     1141 gcaccttggg atggaacacc agagcaatgt cgcgtacggc aatcatttcg agaatggcta
     1201 cctcgggcac gacttgtcgg gaacgggttg gggtctgaaa tgggatttca tcatcgtcca
     1261 cgagagcggc cacgagtggt tcggtaacag catcaccgat aaagatgccg cggacatgtg
     1321 ggtccacgaa ggttttacca actattcgga gaccctgttc accacgtata tgtacggcgt
     1381 cgaagcgggc aacgactatg taatcggtac gcggaagaac atcagcaacg agtctacgat
     1441 catcggcacc tacaacgtca acaaggaagg ctccggggac atgtactata agagcggcaa
     1501 tatgcttcat atgatccggc agatcatggg cgacagcgct ttccgcggca tgttgcacgg
     1561 cctgcaaaaa accttctatc accagacggt gaccacgcag caaatcgagg attattgcag
     1621 ccaatacgcc gggaaggatt tctccaagat cttcgatcaa tacctgcgca cgacgcaggt
     1681 ccccgtgctc acctatcagt ctagcggaga cgtcatctcc taccgctggg tgaactgcgt
     1741 aaaggggttc aacatgccgg tcaaagtcta ctgcggaacc caacccgaaa aatggatcta
     1801 cccaaccgag gactggcaaa ccatgccggc gggtagttcc ggccagatcc tcaggggcgc
     1861 gggcggccag atgcccccgc agacccgcgc cgggcaggcg ggccatcgca gcccgcaggg
     1921 gccgggcccg aagatcgaga ttgtcggcca gacaggtaat ggctcgggcg gcgacggcaa
     1981 cctatccgtt gacagaaact tctatatcat tatgaggaag gccgg
//
LOCUS       DATGKE010000155         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_476827, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000155 DATGKE010000000
VERSION     DATGKE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476827"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..834
                     /locus_tag="VKU83_02705"
     CDS             94..834
                     /locus_tag="VKU83_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92486.1"
                     /translation="MKLFRFHGSLFLAAAVAFLSSCGSGGNEKPAVTDSTAAADSAAK
                     AQAANTIVTTPENMVTITHKVANYDKWLAAYEAHDSARMAAGLHNYVIGRGVQDSNMV
                     LVALKVDDTAKARAFSKDPGLRKAMKKGGVVGAPTIFLSTETWQDTAHLDPTVIRSMV
                     SLTVKDWDAFMKSFEDGKQERADNGIMARVVGHDLDDNKKVAVVVALSDTAKAFAYYR
                     SDTFKKRIESGTVVGQPVRFLFHIVKRY"
     gene            988..>2365
                     /locus_tag="VKU83_02710"
     CDS             988..>2365
                     /locus_tag="VKU83_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92487.1"
                     /translation="MGKEIPAANGSPIASKKTGLSIMDGNEAAAYIAYKTNEICAIYP
                     ITPSSAMGEWADEWSAQGLHNIYGQTPKVIEMQSEAGAAGTVHGALQGGSLSTTFTAS
                     QGLLLMIPNMYKIAGELIPTVFHIAARSLATHALSIFGDHSDVMAVRSTGFSMLFGNS
                     SQEAMDMALIAQAATLRSRIPFLNIFDGFRTSHELTEVEKIPDETIEAMIDMEDVNQH
                     RARALSPDNPFIRGTAQNPDVFFQNREAANRYHAKVVEVVKDKFQKFYQLTGRRYLPF
                     EYYGHEEADRLIVIMGSGAGAVKETVDYLNADGKRVGVLIVRLFRPFSVVDFLAVIPK
                     TVNRVAVLDRCKEPNSIGEPLYMDVVNAFSDDLTHSRPLVTGGRYGLSSKEFTPAMVV
                     AIFQELATGDPKHHFTIGINDDVTQLSLPYDHTFSLDKQHLFRGQFFGLGSDGTVSAN
                     KNSIKIIGT"
BASE COUNT          589 a          653 c          583 g          540 t
ORIGIN      
        1 cggcttactt attatgatta tatttaatag cgctttacct tctgaatcgt tcttacctcc
       61 ctgtttaatt attcactctt aaactcaaaa tcgatgaagc ttttcagatt tcatggctct
      121 ctgtttctgg cagcggcggt agccttcctg tcctcttgtg gttccggcgg caacgagaaa
      181 ccagcggtga cggactctac ggctgcggct gactccgccg cgaaagcgca agcggcaaat
      241 accattgtca ccacgcccga aaacatggtg accatcacac acaaggttgc caattatgac
      301 aaatggttag cagcctatga agcgcacgat tccgcccgga tggccgcggg attacataat
      361 tatgttattg gtcgcggcgt gcaggattcc aatatggtcc tggtcgcgct gaaagtcgac
      421 gacaccgcca aagcaagggc attttccaaa gatcccgggc tcaggaaggc catgaaaaaa
      481 ggaggcgttg tgggcgcgcc gacaattttc ctttctacag agacctggca ggacacggcc
      541 catctcgacc caacggtcat ccgttccatg gtctccctta cggtcaaaga ctgggatgcc
      601 tttatgaagt catttgaaga tggcaagcag gaacgcgccg ataacggtat catggcccgg
      661 gtcgttggac acgacctgga cgacaataaa aaagttgccg tggtggtcgc tctgtccgac
      721 acggccaaag ccttcgctta ttacagatcc gacaccttca aaaagcgaat tgagagcggc
      781 accgtcgtcg ggcaaccggt tagattccta ttccatatcg tcaaacggta ctaaaccctt
      841 taccaccgca cgaataaaaa atgaagcatt tgcttcattt ttttttatgt atggccaggc
      901 aacagtataa atcatcccga cccctgatcc cgatcatctt ttccccacgg tctgcggcct
      961 atatttattt ccaccaaacc aggggttatg ggcaaggaaa taccagctgc caacggcagc
     1021 ccgatagcat ccaagaaaac cggcctttcc ataatggatg gcaacgaggc ggcagcttat
     1081 atcgcctaca agaccaacga gatttgcgcc atttatccca ttacgccttc ctccgctatg
     1141 ggcgagtggg cggatgaatg gagtgcacag ggactccaca atatttacgg ccagacaccg
     1201 aaggtgatcg aaatgcaaag cgaggccgga gccgccggaa ccgtacacgg cgccttgcag
     1261 ggtggcagtc tgtccaccac cttcactgca tcacagggcc tgctgctgat gatacccaat
     1321 atgtacaaga tcgccgggga attgatcccg acagtatttc atatcgcagc ccggtccctg
     1381 gccacccatg cgctatccat cttcggtgac cacagcgacg tcatggcagt tcgctccacc
     1441 ggcttcagta tgctttttgg taattcatcc caggaagcaa tggatatggc gctgatcgcc
     1501 caggccgcca cactccgttc ccgcatcccc ttcctaaata tctttgacgg ctttcgcacc
     1561 tcgcatgaac tgacagaagt tgaaaagatc cccgacgaga cgattgaggc catgatcgac
     1621 atggaagatg tgaatcaaca ccgcgcacgg gctttgtcac ctgataaccc gttcattcgt
     1681 ggtacggcgc aaaacccgga cgttttcttt caaaacaggg aagctgccaa ccggtatcat
     1741 gccaaagtgg tcgaggttgt gaaggacaaa ttccaaaagt tctaccaatt gaccggcagg
     1801 aggtatctgc cgttcgaata ttacggccac gaagaagccg accgcctgat cgttattatg
     1861 ggctcgggag caggcgctgt aaaagaaacc gtcgattacc tgaacgccga cgggaagcgg
     1921 gtcggtgtgc tcattgtaag gctgttcagg ccgttctccg ttgtcgattt cctggccgtc
     1981 attcccaaga cggtaaacag agtggccgtg ctggaccggt gcaaagaacc caacagcatc
     2041 ggcgagcctt tgtacatgga tgttgtaaat gctttcagcg acgacttaac gcattcccgg
     2101 ccgctggtga ctggcggacg gtacggtttg tcatcaaaag aattcacccc cgccatggtg
     2161 gtagccatct tccaggagct tgcgaccggg gatcccaaac atcattttac tatcggtata
     2221 aacgatgatg tcacccaact gagcctgcct tacgatcata ccttttcatt ggataagcaa
     2281 cacctgttcc ggggccagtt tttcggcctt ggttcggacg gcacagtaag cgccaacaaa
     2341 aactccataa agatcatcgg aacca
//
LOCUS       DATGKE010000156         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1310767, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000156 DATGKE010000000
VERSION     DATGKE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310767"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VKU83_02715"
     CDS             complement(<1..1172)
                     /locus_tag="VKU83_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92488.1"
                     /translation="MNRKFRYFCLLAAAAIAACHPAGKYSSWPAYGGAANIHYSDLRE
                     IDTGNVAGLREAWTYHTGDADSNSQIQANPIIIGHTLYGVSPRLKLFALDAATGRLQW
                     SFDPATVTSVGIRSLNTCRGVAYYRGSESDQRLFFAAGHSLYCVDALTGRLVPGFGEQ
                     GRIDLHNDLGRDVSDLYIAATTPGVVYKDMLILGDRVAEDADAAPGHIRAYDVHTGRL
                     RWIFHTIPYPGEAGYNSWDDTAAYRHIGGANDWSGFSLDAERGIVFAAVGSASADFYG
                     GRRLGNDLFADCALALDANTGKLIWHFQTIHHDLWDRDPPTPPVLVTVKKEGKSIAAV
                     AQVTKSGLVYLLDRSTGKPIYPVVERPVPDSSELAGERPAPTQPWPTVMPPFARQSV"
     gene            complement(1265..2677)
                     /locus_tag="VKU83_02720"
     CDS             complement(1265..2677)
                     /locus_tag="VKU83_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLI92489.1"
                     /translation="MMKTSARLLSRILPVILVFLFVDVSCKKHSDGPFVAPTALPAID
                     LELNGFTADWTKIGPAASYNLFVATDTGFTQPIAGYNPMSVKALNQVVSGLSPKSLYY
                     YRLQAVNSGGTVTAYSNTEPVTTADTLDDHYVYIGSEDENFYCFLALTGTKAWSFPTK
                     GDIESSPTLVGGSVYFGGTDQRLYALDAFTGQKRWNALAQGAIMSSPTYSNGAVYFDS
                     YAGFVIRADTVNGHEDWNVLLNGSKQLLFSSPTVVNGLLYVGGQDHNLYALDPSTGNR
                     VWAAPTNDTISSSPAVSNGIVYVGCSDWHMYAFNAANGNLIWRNSTGDSIVSSPTVAN
                     GMVYVGSYDDGVYAFDALNGNRFWRAPTGGRVQSSPTVAGGVVYAGSFDGKLYAFDAQ
                     SGALIWSAATGDRILSSPVVGNKAVYVGSQDHKLYAFDIKTGAILWAAPTGGRIRMAS
                     PLVYTYNGNVYHPGVSGDQQ"
     gene            2922..>4789
                     /locus_tag="VKU83_02725"
     CDS             2922..>4789
                     /locus_tag="VKU83_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLI92490.1"
                     /translation="MNLSTSGEEAELIPEFLSGGGEMGQRIREFDWSSTSLGPVNAWP
                     QSLRTCIRIMLGSRQPIWIGWGKDLIKLYNDPYRDIVRGKHPWALGKPAAVVWREIWP
                     TIGPMLHQVMEKDEGTYVESQFLAMERNGYPEETYYTFSYTPIPGANGGTAGMICFNT
                     DDTERIISERQLNTLTSLGKRLTDSQSAPDVIRKTVDTLRENPFDFPIAGFYLVKGDK
                     ACLTASTEQPGTTEFTEGGNTGKSAHGTDPPLGSRLPAEIDLNGRYGISAAVKQALQK
                     KQPQIVDDAADYLGRLQGGPWELPPSNIMILPFTQTGEADPYGLLVTGLNPYRLPDEK
                     YRGFFSIIADQVTSSFGNVHVLEQERKRAQALAEIDLAKTTFFSNISHEFRTPLTLLL
                     GPIEDALRDPDDVTQNRLRMGVAYRNALRMQKLVNALLEFSRIEAGRLEGQFSRVDVC
                     ALTADLVSSFRSAIEKAGMSLKLDLEPVTAEVYVDPAMFEKIILNLVSNAFKYTSEGE
                     IGVAVRERNGEVRISVTDTGIGIPEDQLHRIFDRFHRVENATVRTQEGTGIGLALVKE
                     LVRIHEGRIEVESNPGQGSTFTVVLPSGKNHLAPDRIISPESIPLTRERNIYVEES"
BASE COUNT         1085 a         1353 c         1398 g          953 t
ORIGIN      
        1 accgactgtc gcgcaaacgg cggcatgacc gtcggccagg gctgcgtggg agcgggacgt
       61 tcgccagcca gctcactgct gtcagggacc ggtctttcaa ccaccgggta gatgggtttg
      121 ccggtggacc ggtcgagcag atagaccagg cccgacttgg tgacctgcgc cactgcggca
      181 atcgacttcc cttccttttt caccgttacc agtacagggg gagtgggcgg atcgcggtcc
      241 cagaggtcgt ggtgaatggt ttggaaatgc cagatcagct tgccggtgtt ggcatccagc
      301 gccagggcac agtcggcaaa aagatcgttg ccgagccgcc ggccaccata gaaatcggcg
      361 ctggcggatc ctaccgcggc gaagacgatg ccgcgctccg catcgaggct gaatccggac
      421 cagtcgttcg caccgccgat gtgccggtag gcggcggtat catcccagga gttgtaccct
      481 gcttcgccgg ggtatgggat ggtgtggaaa atccaccgga ggcggcccgt atgcacatca
      541 tatgcccgga tatgaccagg cgccgcatct gcgtcctcag caacccggtc gccaaggatc
      601 agcatgtctt tatacacgac tcctggcgtg gtggcggcga tatacagatc gctgacgtcg
      661 cgtcccaggt cgttgtgaag gtcgatccgg ccctgttcgc caaaaccggg cacgagacgc
      721 cccgtcaggg cgtcgacgca gtacagcgaa tgccccgctg cgaaaaacag gcgctgatca
      781 gactcgcttc cccggtaata ggccactccc cggcaagtat tgagcgatcg gatcccaacc
      841 gaagtaacgg tcgccggatc aaagctccac tgcagccggc cggtggcggc atcgagtgcg
      901 aacagcttga ggcgggggga tacgccatac aacgtatggc cgataatgat ggggttggcc
      961 tggatctgcg aattgctgtc ggcatcgccg gtatgatagg tccaggcttc acgcaggcct
     1021 gccacgttgc cggtgtcgat ctcgcgcagg tcggaatagt ggatattggc ggcgccccca
     1081 taggcgggcc aggagctata ttttcctgct gggtggcaag cggcgatcgc cgccgcggcg
     1141 agcaggcaga aatagcgaaa ctttctgttc atcggtggga ggaatagtga atggacggca
     1201 tttatcgcct caatttatca ttatgtcggc aaataaacaa tcctttttgc tgatggcagc
     1261 acacctattg ctggtctccg ctgacacccg ggtgatagac attcccgtta tacgtgtaga
     1321 caagtggcga tgccatccgg atcctgccac cggtcggtgc tgcccagagg atcgctcccg
     1381 tcttgatgtc gaaggcatac agcttgtgat cctgtgaccc gacgtaaacg gctttgttcc
     1441 cgacgaccgg gctggacagg atgcggtctc cggtggcggc actccagatc agggcgccgg
     1501 attgggcgtc aaaagcatac agtttgccgt cgaaactgcc cgcatagaca accccgcccg
     1561 caacggtcgg gctcgactgg acccggcctc ccgtgggagc ccgccagaac ctgttcccgt
     1621 tcaaggcatc gaaagcgtac acaccgtcat cgtagctgcc gacatacacc attccgttcg
     1681 caaccgtcgg ggaagagacg atgctatcgc ccgtggagtt tcgccagatg aggtttccgt
     1741 tagcggcatt gaacgcatac atgtgccagt cgctgcagcc gacatacacg atgccattgc
     1801 tgaccgccgg agaagaactg atggtgtcgt tggtaggcgc tgcccatacg cgattgcctg
     1861 tcgagggatc cagcgcataa aggttgtggt cctggccgcc gacgtataat agcccgttga
     1921 cgacggtcgg gctggagaac aacaactgtt tgctgccatt caagaggaca ttccagtcct
     1981 cgtggccgtt gacggtgtcg gccctgatca caaacccggc atagctgtcg aagtagactg
     2041 cgccgttaga gtaggtcgga ctgctcatga tagcgccttg tgccagggca ttccaccgct
     2101 tctgccccgt aaaggcgtcg agggcataca accgctggtc ggtaccccca aagtaaacgg
     2161 aaccgccgac cagggtcggg ctggattcga tgtcaccctt cgtcgggaaa ctccaggctt
     2221 tcgtgccggt taacgccagg aaacagtaga aattctcatc ctcgctgccg atatagacat
     2281 agtggtcgtc cagggtgtcg gcagttgtca ccggttccgt gttcgaatag gccgtgacgg
     2341 tgcccccgga attcacggcc tgaagccggt agtaataaag tgacttcggc gacagtccgg
     2401 aaaccacctg gttcaatgcc ttgaccgaca tgggattata tcctgctatg ggctgggtga
     2461 accccgtgtc cgtcgccaca aaaagattat aactggccgc agggccgatc ttggtccagt
     2521 cggcggtgaa tccattgagt tccagatcga tggcggggag agccgtcgga gcaacaaagg
     2581 ggccatcgct atgtttctta cacgaaacat ctacaaagag gaacacaagg atgaccggga
     2641 gaatcctcga caataagcgc gcggatgtct tcatcataca tttcgtgtta attcaatgtc
     2701 cggcttaatc aagggaattg gggcaggata ttgagggaaa agggccatgc ccaaacggga
     2761 cactctaaga aggatattgc cccgggggca attagaattt agcgacggcc tggtggcgag
     2821 ggacgaaaat acgtaatagt tactggatgt caaaatcggc gtttatggcg ggggcatcct
     2881 ttttgcacaa ttgcatgctt aaaaaaacca taacttctat catgaatctg tccacatccg
     2941 gagaagaagc tgagcttatc cctgaattcc tgtccggtgg cggtgaaatg ggccaaagaa
     3001 taagagaatt tgactggtcg tccaccagtc ttggtccggt gaacgcatgg ccccaaagcc
     3061 ttcgcacctg catccggatt atgctgggtt cccggcaacc catctggatc ggctggggta
     3121 aggacctcat caagctctac aacgaccctt acagggatat tgtacgcggc aagcacccct
     3181 gggctcttgg aaagcccgcg gcggtcgtct ggcgggaaat ctggcctact atcggaccca
     3241 tgcttcacca ggttatggaa aaggatgaag gtacgtacgt agaatcccag ttcctggcga
     3301 tggagcgaaa cggatatcct gaggagacct actatacctt ttcttatacc ccgataccgg
     3361 gtgctaacgg tggtaccgcc ggaatgatct gtttcaacac cgacgacacc gaacgcatca
     3421 tcagcgaaag acaactcaat acgctgacca gtctgggtaa gcgtcttact gattctcagt
     3481 ctgccccgga cgtcatccga aagacggtcg atacgctgcg cgaaaatcct tttgatttcc
     3541 ctattgccgg tttttacctc gtcaaaggcg acaaagcctg cctcaccgcc agcacggagc
     3601 agccggggac gaccgaattt acggaaggcg gaaacaccgg taaaagcgcg cacgggacag
     3661 accctccgct cggctcccgc ctgcccgcgg aaatagacct gaacggtcgc tacggaatct
     3721 ccgcggcagt gaaacaagcc cttcaaaaga agcaaccgca aattgtcgat gacgcggcgg
     3781 actacctcgg aaggctgcaa ggaggcccct gggaactccc cccatccaac atcatgatcc
     3841 tgccattcac tcagaccggc gaggcggacc cttatggttt gctcgtgacg gggctcaatc
     3901 cgtaccggct gccggatgaa aagtaccggg gctttttttc catcatcgcc gaccaggtca
     3961 ccagcagctt cggaaacgtc catgtcctgg aacaggaacg caaacgggca caagcccttg
     4021 ccgagattga cctggcgaag acgacatttt tctccaatat cagccacgaa ttccgtaccc
     4081 cgctgaccct gttgcttggc ccgatcgaag acgcgcttag ggatccggac gacgtgacgc
     4141 aaaaccggct gcggatgggc gtcgcgtacc ggaatgccct gaggatgcaa aagctggtaa
     4201 atgcgctgct tgaattttcc cgtatcgagg cgggccgcct ggaaggccaa ttcagccggg
     4261 tcgacgtctg cgccctgacc gcggacctgg tgagctcatt ccggtcggcg atcgaaaaag
     4321 ccggcatgtc gttgaagctc gacctggagc cggtgacggc tgaagtatat gtcgatcccg
     4381 ccatgttcga aaaaatcatc ctaaacctgg tttccaatgc attcaaatat accagcgagg
     4441 gcgagatcgg tgtagcggtg cgggaacgaa atggagaagt acggatatcg gtaacagata
     4501 ccgggatcgg catcccggag gaccaactgc accggatatt tgaccgcttt caccgggtgg
     4561 aaaatgcaac ggtgcgcacc caggaaggca ccggcatcgg tcttgccctt gtcaaagaac
     4621 tggtgcggat ccacgagggg cggattgagg tggaaagcaa tcccggccag gggtcaacct
     4681 tcaccgtagt actgccttca ggcaaaaacc atctggcgcc cgaccggatc atttcccccg
     4741 aatcgatccc gctgaccagg gaacggaaca tctatgtcga agaatctat
//
LOCUS       DATGKE010000157         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_792637, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000157 DATGKE010000000
VERSION     DATGKE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792637"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VKU83_02730"
     CDS             <1..754
                     /locus_tag="VKU83_02730"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HLI92491.1"
                     /translation="GGNNRVILLTDGDISMEPPAKEQDVEDLVAQGIQAGIRLTCGGL
                     GMKSVKDSRLPVLAEIGRGDFAYLDDEGVTEQLLADQLDPRLASVAENVSLSVDFSPA
                     LVSEYRLIGYNNKRNAITDSAEAITGARVGSGSSQVALFEFAPKVDTTGADTLARLKV
                     NYSLPGQNLIRSLAFSCMNDVEPVERAEPEWRRAICLVMFGMKLRHSDYLGSVTWPDI
                     ERMAKRLFTGNNYLDDEYISLIDRAKRIYSRE"
     gene            968..>4800
                     /locus_tag="VKU83_02735"
     CDS             968..>4800
                     /locus_tag="VKU83_02735"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLI92492.1"
                     /translation="MRKIWAIALVCGLIQASDAAAQIHHFALSSSEFLLDGKPFQIIS
                     GEMHPARIPREYWLQRIRMAKAMGCNTIAAYVFWNYLEPEPGQWDLRTGNHDIAAFIR
                     LCQAEGMWVLLRPGPYVCAEWDWGGLPTWLLKFPDIKVRCMDRRYMDAVSKYIARLSR
                     EVVPLQCDHGGPILMVQVENEYGSYGNDRAYVKALRRLWQEHGVTVPFYTADGPTPFM
                     LEAGSLDSCAIGLDSGGSDADFEQATKQNPHVPAFSSETYPGWLTHWKERWQHPDTAD
                     IMNEVTYLLSRHRSFNLYVVHGGTNFGFTAGANAFSPTEFQPDVTSYDYDAPINEQGR
                     ATPKYYALRRLIGEYSDHSLPPVPAPVPAINIPPMQLKPVASLWDNLPAPKLTPTPMP
                     MESFGQGSGLILYRTRLIGRKSGELRITEPHDFAMVFLNGKLVDTVYRDGGHWTVKIP
                     ESGVREPVLEILVEAMGHINFAQYMIDRKGITDRVTLDGMTLMNWETYNLPMTPGWVR
                     GMKSIFTNPAAAERATAGRNARPGLFFKAMFNLDTVADTYVDMSGWRKGIVWVNGHNL
                     GRYWNVGPQERLYCPAPWLKKGLNEMMVFDLLKPDGGTVRGAETLEEGDAPARGATGR
                     VEGEAANGEDSQGAGVQWVARPDITRVNRYYLSNRAPLEPLSFLKLPVGSIEPQGWVR
                     KYLELQRDGLTGHLGEISAWLDKKDNAWYSGNGEGSHGWEEVPYWLKGYGDLGYLLHD
                     SGMIRTTRDWLGKVFESQRPDGYFGPRVVDQPEKDSIPDLWPNMLMLWCMQSYYEHSG
                     DQRVLQFMTRYFRWELSVPQDLLLRTYWENSRGGDNLYSIYWLYDHTGESWLLELANR
                     IHHHTANWSQDTTLPNWHNVNVAQSFREPATYFMQVKDSSYLQSTYDDFYLIRRLYGQ
                     VPGGMFGADEDARKGFTDPRQGVETCGMVEQMASDELLIGITGDPMWADNCEDVAFNT
                     YPAAVLPDFRGLRYLTAPNMVVSDDKNHSPGIENAGPFLLMNPFSSRCCQHNHSQGWP
                     YYAEHLWMATPDEGVAALLYSSSSVHARVGNGEDVRIEETTHYPFEETVRFRIGINQP
                     TAFPLYLRIPGWCQGAKVRVNGRESAVALAPDGFARIRKVWTEGDEIELELPMKVRVR
                     TWDQNKGSESVNYGPLTFSLKIKEDYVKVDSKANAIGDSKWQASADQSKWPAFEILPG
                     SAWNYGLVAGDSFKIVRRPWPADDFPFTQQSAPIEIRTTGRQVPGWTIDQYGLCGVLP
                     VSPVAVNTPEQSLVL"
BASE COUNT         1070 a         1163 c         1573 g          994 t
ORIGIN      
        1 gggcggcaat aaccgcgtca tcctgctgac cgacggggat atcagtatgg aaccgccggc
       61 aaaggagcag gacgtggaag acctggtggc gcagggaatc caggctggca tccggcttac
      121 ctgcggcggc ctggggatga agtcggtaaa ggattcgcgg ctgcccgtgc ttgccgagat
      181 cggccgtggg gacttcgcct acctggacga tgagggtgtg accgagcagc tgctggcgga
      241 ccagctcgac cccaggctcg ccagcgtggc ggagaatgtg tccctgtcgg tggacttcag
      301 tccggcgttg gtaagcgagt accggctgat cgggtataac aacaagcgca acgccatcac
      361 cgattcggcg gaggcaatta ccggcgcaag ggtcggatcg ggtagttcac aggtagcctt
      421 gttcgaattt gcgccgaagg tggataccac cggggcggat acactagcac gcctgaaggt
      481 aaattattct ttgcccggac agaacctaat ccggtccctt gccttcagtt gtatgaatga
      541 tgtcgagccc gttgaacggg cggagccgga gtggagacgg gcgatctgcc tggtcatgtt
      601 cggaatgaaa ttgcgtcatt cggattatct tggttccgtg acctggccgg atatcgaaag
      661 aatggccaag cggctcttta ccggaaataa ttatttagat gacgagtata tttcactgat
      721 agacagagca aaacgaattt atagtcggga gtaagtgcgc cctccggtgc gcctttccag
      781 ctcatggcaa cgaaagctgt cagagcagga cggatcgaca atcgcgatgc cggctgcctc
      841 cccggatttc ttaaacgaga agagtggttg gcttatacta aattagcaaa tcgctgcatg
      901 cggacatgcg gaaatctccg cggaaggcat gccgggcgga tggattattt gaataagaca
      961 gctacacatg agaaaaattt gggcaattgc actggtttgc ggcctcatcc aagcgagcga
     1021 cgccgctgcg cagattcatc attttgcttt gagcagcagt gaattcctgc tggacggtaa
     1081 gccgttccag atcatcagcg gcgaaatgca cccggcgcga atcccgcgcg aatactggct
     1141 acagcggatc cggatggcga aggcgatggg ttgcaatacg atcgccgcat acgttttctg
     1201 gaattacctg gagcctgaac cggggcaatg ggatctccgg acgggcaatc acgatatcgc
     1261 cgcattcatc cgcttgtgcc aggctgaggg aatgtgggtc ttgttgcggc cggggcctta
     1321 tgtgtgtgcg gaatgggact ggggtgggtt acccacctgg ctgctgaagt tccctgatat
     1381 caaggtgcgg tgcatggacc gccgctatat ggatgcggta agcaaatata ttgcacggtt
     1441 atccagggag gtggtgccgc tgcagtgtga tcacggcggt cccattctga tggtccaggt
     1501 agagaacgaa tacggcagct atgggaatga ccgggcctat gtcaaagcct tacgccgatt
     1561 atggcaggag catggcgtaa ccgtaccttt ttatacagcg gatgggccga cgccgttcat
     1621 gcttgaggcc gggagcctcg atagctgcgc gatcggactg gacagcggcg gcagtgacgc
     1681 cgattttgag caggcgacca aacaaaatcc ccacgtaccg gctttcagca gcgagaccta
     1741 ccctggatgg ctgacacatt ggaaggaacg ctggcagcat ccggacacgg cggatatcat
     1801 gaatgaagta acatacctgc tgtcgcgtca ccggtccttc aatttatatg tggtgcatgg
     1861 aggaacgaat tttgggttta cggcgggggc aaacgccttt tcaccaacgg aatttcagcc
     1921 ggatgtaacg agttatgatt atgatgcgcc tatcaacgag cagggaaggg cgacgccgaa
     1981 atattatgcg cttcggcgat tgatcggcga gtactccgac cattctttac cgcccgtgcc
     2041 ggcgcctgtg ccggcgataa acattccgcc gatgcaattg aagccggtgg cttccctctg
     2101 ggacaattta cctgcaccga agttgacgcc aacccccatg ccgatggaat cattcgggca
     2161 gggcagtggc ctcatcctgt accggacgag attgatcggg cgcaaaagcg gtgagctgag
     2221 gatcacggag ccgcatgact ttgcaatggt ttttctgaac gggaagctgg ttgatacggt
     2281 atatcgcgac ggtggccact ggacggtaaa aatccctgaa agcggcgtca gggagcctgt
     2341 gctggagata ctcgtggagg cgatgggcca tatcaatttc gcccagtata tgatagaccg
     2401 gaaaggcatc acggaccggg tgacactgga cgggatgacg ctgatgaact gggagacgta
     2461 taatttgccc atgacacccg ggtgggtgag agggatgaag tcgatcttta cgaatccggc
     2521 ggccgcggaa cgggcgacgg ccgggcggaa tgcgcgaccg ggcttgttct ttaaggcaat
     2581 gttcaacctg gacacggtgg cggataccta tgttgatatg tccggatggc ggaagggcat
     2641 cgtttgggtg aatggacata acctcggaag gtattggaat gtggggccgc aggagcggtt
     2701 gtattgtccg gctccatggt tgaagaaggg gcttaacgaa atgatggttt ttgatctgtt
     2761 gaagccggac ggcgggacgg tgaggggggc ggagacattg gaggaggggg atgccccggc
     2821 acgcggcgcc acgggccgcg ttgagggtga agctgcgaac ggtgaggaca gtcagggtgc
     2881 gggagtccag tgggtcgccc ggccggatat cacccgggtg aaccgctatt atctgtcaaa
     2941 ccgggcgccg ctggagccgt tgagctttct gaaactgccg gtgggaagca ttgaaccgca
     3001 aggatgggtg aggaagtatc tggagctgca acgcgacggg ttgaccgggc acctgggcga
     3061 gatcagcgca tggctcgaca aaaaagataa tgcgtggtac agcggcaacg gcgaaggttc
     3121 gcatggatgg gaagaagtgc cgtattggtt gaaaggctat ggggaccttg gttacctgct
     3181 tcatgacagc ggcatgatcc gaacgacacg cgactggctc gggaaggttt ttgaaagtca
     3241 gcggccggac ggatatttcg gtccgcgggt cgtcgaccag cccgaaaaag attcgattcc
     3301 cgacctatgg cccaacatgc tcatgttgtg gtgcatgcag tcctattacg agcatagcgg
     3361 tgaccagcgg gtgctgcagt ttatgacgcg ttattttcgt tgggagctga gtgtaccgca
     3421 ggacctgttg ctgcggacct attgggagaa cagccgggga ggcgataact tgtacagcat
     3481 ttattggctg tatgatcata cgggggaaag ctggctcctt gagctggcga accggataca
     3541 ccatcatacg gcaaactggt cccaggatac gacgcttccc aactggcata atgtcaatgt
     3601 tgcgcaaagt ttccgggagc ccgccaccta ctttatgcaa gtgaaggact catcctattt
     3661 gcaatccacg tatgacgact tttacctgat ccggcggcta tacgggcagg tgccgggagg
     3721 gatgttcggc gcggacgagg atgcccgtaa aggtttcacg gacccgcggc aaggtgtgga
     3781 gacatgtggc atggtcgagc agatggcgtc ggatgaattg ttgataggca tcacgggtga
     3841 cccgatgtgg gcggacaact gtgaggacgt ggccttcaac acctatccgg cggcggtgct
     3901 gccggacttc agggggctgc ggtatcttac tgcgcccaac atggtggtca gcgatgacaa
     3961 aaatcacagc ccgggaatcg agaatgcggg ccctttcctt ttgatgaacc ctttcagcag
     4021 ccgctgctgc cagcacaacc attcgcaggg ctggccttat tatgcggaac atctgtggat
     4081 ggcgacgccg gacgaagggg tggctgcgct gttatacagc agctcgtcgg tgcatgcgcg
     4141 cgtcggcaac ggcgaagacg tgcggatcga ggagactacg cattatccct tcgaagagac
     4201 ggttcgattc aggatcggca tcaatcagcc gacggcattt ccactctatc tgcggatccc
     4261 gggctggtgc cagggcgcga aggtgagggt gaatgggcga gagagcgcgg tggcgctggc
     4321 gccggatggg tttgcgagga tccggaaggt ttggacggag ggtgatgaaa tcgaattgga
     4381 gctgccgatg aaagttcgcg tcagaacctg ggaccaaaat aagggaagtg aaagcgtgaa
     4441 ttatgggccg ttgacctttt ctttgaagat aaaggaggat tatgtaaaag tggacagcaa
     4501 ggcaaacgcg attggcgact cgaaatggca ggcaagcgcc gaccagtcga agtggcccgc
     4561 gttcgagatc ttgccgggaa gcgcctggaa ctatgggctt gttgccgggg acagttttaa
     4621 gatcgtgcgc aggccatggc cagcggatga ttttccgttc acgcagcagt cggcgcctat
     4681 agagatccgg acgaccggac ggcaggtccc cggttggaca atcgatcaat atgggctatg
     4741 cggcgtcctg ccggtcagcc cggtcgcggt caatacgccg gagcaatcgc tggtactcat
//
LOCUS       DATGKE010000158         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_476667, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000158 DATGKE010000000
VERSION     DATGKE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476667"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VKU83_02740"
     CDS             <1..602
                     /locus_tag="VKU83_02740"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLI92493.1"
                     /translation="RSLGAYANIFAIESFMDELAAKAGKDPYEFRLRHLHDPPAIAVL
                     KKLRELVGQSNAGIAFSRYKNGGTYCAVAANVSVKANKVTVHKLWAVVDAGEVINLDG
                     IRNQIEGGLVQSASWTLFEEVKFDPEQITSTAWASYPILHINQAPEVEVIALDHPDEQ
                     PMGAGEAAQGPAAAAILNAIHNACGKRIRRLPVSTAILI"
     gene            complement(573..1304)
                     /locus_tag="VKU83_02745"
     CDS             complement(573..1304)
                     /locus_tag="VKU83_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLI92494.1"
                     /translation="MTVRKFDSREGMGQAAAEAIAAAVGAVLRGKEVVRIVFAAAPSQ
                     KEVLRALAESEMAWGRVIAFHMDEYLGLEPGAPQGFGNFLRDRLFSKVPLKQVHYLNG
                     QAADPLEECRRYARLLEQHPPDIVVLGIGENSHLAFNDPHAALFDDPLTVKVVELDAN
                     CRRQQVNDGCFATIEEVPTHALTLTIPALMQAGSVFGIVPGKRKAAAVWHTLHSEICE
                     RYPSTILRRHPNVALYLDEDSGGHG"
     gene            complement(1285..2529)
                     /gene="nagA"
                     /locus_tag="VKU83_02750"
     CDS             complement(1285..2529)
                     /gene="nagA"
                     /locus_tag="VKU83_02750"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HLI92495.1"
                     /translation="MKLPLPKRRTLKIYNGRILTPDRVIPKGTVVVREGKIVEVVEGD
                     CQAGDAIAIDARGMYISPGFIDMHVHGGGGHDFMDGTVEAFLRIAETHARYGTTALMP
                     TTLTSTKEALFGVFERYKEAVAQNTRGARFLGLHLEGPYFAMSQRGAQDPRFIRHPDP
                     VEYREILARSAAGPGEAPAPYLIKRWSAAPELEGAIPFARFLRSSGVLVALAHTDALY
                     EEALKGFENGFTLATHFYCCMSGVTRRNAYRYAGAIECAYLTEMDVEVIADGVHVPAP
                     LLQLIYKIKGPERTALVTDAMRAAGMPEGESVLGNLHDGLRVIVEDGVAKLPDRGSFA
                     GSVATADRLVRTMIKEAGVSLSEAVRMITATPARILGVGDRLGALAPGMEADIVLFND
                     DIEVDMTIVGGKIIYANDCKEV"
     gene            2691..>3655
                     /locus_tag="VKU83_02755"
     CDS             2691..>3655
                     /locus_tag="VKU83_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92496.1"
                     /translation="MKTNIVLTLATTAVVAGLVIACNKTNSTSGNTGPNSDNTSTTQM
                     QTQADDESQVNNELNDAETDVNNSLNASTTFSGTTGAGSESGIVTTGGNGGIIIDYGI
                     CDATVTTDTANGLRQLVITYNGLNCRGNRTRTGVVTISIPVGVRWRDSGAVVTVNIDN
                     LKITRVRDNKTITLNGTYTFTNVTGGLLRDLASRGTITHTITADSVTIQFADSATRVW
                     SVAKKRVFTYNDGVVITTTGTHSDGTNTDVAVWGTNRYGNSFETLILQPLVIQQSCDW
                     RLTSGETETIRPAVTLTVTYGLDSNGNPTSCPGSGWYYFKEVWTR"
BASE COUNT          877 a         1228 c          815 g          735 t
ORIGIN      
        1 tacgtagcct cggcgcctat gccaacattt tcgctatcga gtcttttatg gatgagctgg
       61 cagcaaaggc aggcaaggac ccatacgaat tccgcctccg gcatctccac gacccccccg
      121 ccatcgccgt ccttaaaaaa ctgcgggagc tcgtcggcca atccaatgcg ggcatcgctt
      181 tctcccgata caaaaacggt gggacctatt gcgctgtcgc cgccaacgtt agcgtcaagg
      241 ccaataaagt cacggtacat aaattatggg ccgtcgtcga cgccggcgaa gtcatcaacc
      301 tcgacggcat ccggaaccag atcgaaggcg gcctcgtcca atccgccagc tggacacttt
      361 tcgaagaggt aaaattcgat ccggaacaga tcaccagcac cgcttgggcc tcctatccca
      421 ttctccacat caaccaggcg ccggaagtgg aagtgatcgc cctcgaccac ccggacgaac
      481 agccaatggg tgcaggcgaa gcagcccagg gacccgccgc agcagccatt ctcaacgcca
      541 tccataatgc ttgtggaaaa aggatccgcc ggctacccgt gtccaccgct atcctcatct
      601 aggtacaacg ctacattcgg atgtcttcgt aagatcgtgg aaggataacg ttcacaaatt
      661 tcactatgca acgtatgcca taccgccgcc gccttgcgct tccccggcac tatgccaaac
      721 actgaaccag cttgcattaa cgccgggata gttaatgtaa gcgcatgtgt cggcacctct
      781 tcgatcgttg caaaacagcc gtcattcacc tgctgccgac ggcaattggc atccagctcc
      841 actaccttga cggtcaatgg atcgtcaaat aacgccgcat gcggatcgtt gaaggccaaa
      901 tgcgaatttt caccgatgcc caacactacg atatccggcg gatgctgctc cagcaaccgg
      961 gcatatcgcc ggcattcctc caatggatca gccgcttgcc cattcagata atgtacttgc
     1021 tttaatggca ctttggaaaa aagccggtcc cgtagaaaat tcccgaaacc ttgcggcgcc
     1081 cccggctcca accccagata ttcatccata tgaaaggcga tcaccctgcc ccacgccatc
     1141 tccgactccg ccaatgcccg caacacctcc ttctgtgatg gcgccgcggc aaatacgata
     1201 cgaacaacct ccttcccccg taataccgca ccaaccgccg cggcaattgc ttccgccgca
     1261 gcttgaccca tcccctcccg gctgtcaaac ttccttacag tcattggcat aaatgatttt
     1321 tccgcccacg atggtcatat ccacctcgat gtcgtcgttg aatagcacga tgtccgcttc
     1381 catgcccggc gccaacgctc caagacggtc ccctactccc aggatccgtg caggcgtagc
     1441 agttatcatc cgcaccgctt ccgaaagcga taccccggcc tccttgatca tggtcctgac
     1501 cagccggtcc gccgtggcca cactgcccgc aaacgaaccg cgatcgggca gcttcgccac
     1561 cccatcttct acgatgaccc gcagaccatc gtgcagattc cccagcacac tttctccctc
     1621 cggcatcccc gccgcccgca tcgcatccgt gacaagcgcg gtccgctccg gaccctttat
     1681 tttatagatc aactgaagta acggcgccgg aacatgtacg ccgtccgcta tgacctcgac
     1741 atccatctcg gtcaaatagg cgcattcgat cgcgccggca taccggtagg cattccgccg
     1801 ggtaaccccg ctcatacaac aataaaaatg tgtcgccaaa gtgaatccat tctcaaaccc
     1861 tttcagtgcc tcttcataaa gggcatctgt atgcgccaac gctaccagca caccgctgga
     1921 tcgtaagaac cgcgcgaaag gaatagcccc ttccagttcg ggcgccgcgc tccatcgctt
     1981 gatcagatac ggtgcaggag cctcccccgg acccgccgcc gaccgcgcca gtatctctcg
     2041 gtattccacg ggatcaggat gacgtataaa ccggggatcc tgcgcccccc gctgactcat
     2101 cgcaaagtac ggcccctcca gatgtaaccc caggaaccgg gccccccttg tattctgcgc
     2161 cacggcctcc ttatagcgct caaatacccc gaacaacgcc tccttcgtgc tcgtcagcgt
     2221 cgtgggcatt aacgccgtcg tcccatatcg cgcatgcgtc tccgcgatcc gcaggaatgc
     2281 ttcgacagtt ccatccatga agtcatgccc tcctccccca tgcacatgca tatcgataaa
     2341 acccggagag atatacattc ccctcgcatc tatggcaatc gcgtctcctg cttgacaatc
     2401 cccttccacc acttccacga tcttcccctc ccggaccacg accgttcctt tgggaatgac
     2461 acgatccgga gtcaggatgc ggccgttata aatctttagc gtccggcgct ttggcaaagg
     2521 caatttcatt ccacaaagat tctgtaagac ggaccaaacc acaaaaagtt taatgcaata
     2581 atctgaaaag caggaatatt ttatgaaagg aaaatatttt atacatccgg gttaaaccaa
     2641 accctttagc gcgcatctga acctacacac aactgttaaa aaaacaaatt atgaaaacga
     2701 acatcgttct cacgctggcg acaacagctg tagtcgccgg cctggtcatc gcttgcaaca
     2761 agaccaactc cacttcgggc aatactgggc ccaattccga caacaccagt actacacaga
     2821 tgcaaacaca ggcggatgat gaatcccagg tgaacaatga attgaatgac gccgaaaccg
     2881 atgtcaacaa ttcgctcaat gccagcacta ccttcagcgg cacaacgggc gccggctcgg
     2941 aaagcggtat cgttaccacc ggcggtaacg gcggcatcat catcgactat ggcatctgcg
     3001 acgcaaccgt cactaccgat acggccaacg gccttcgtca gctcgtcatc acgtacaacg
     3061 ggctcaattg ccgcggtaac cgtacccgca ccggcgtggt cacgatctct attcccgttg
     3121 gcgttcgctg gcgtgattcg ggtgccgtcg tcactgtcaa catcgacaac cttaagatca
     3181 cccgcgttcg cgacaacaaa accattaccc tgaacggtac ctataccttt accaacgtca
     3241 ctggcggcct gctcagagac ctggccagcc ggggtactat cacgcacacg atcacggccg
     3301 acagcgttac catccaattc gcagacagcg cgacccgcgt ctggagcgtt gctaaaaaac
     3361 gcgttttcac ttacaatgac ggtgttgtga tcaccactac cggcacccat tccgacggca
     3421 cgaataccga cgtcgccgtc tggggtacga accgctatgg caattccttc gaaacgctca
     3481 tcctccaacc cctcgtcatc caacaatcct gcgactggcg gctgaccagc ggcgagaccg
     3541 agactatccg ccccgccgtt acccttacag tcacctacgg cctcgattcc aacggcaatc
     3601 ccaccagctg ccccggatcc ggttggtatt atttcaaaga ggtctggacc aggaa
//
LOCUS       DATGKE010000159         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1310410, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000159 DATGKE010000000
VERSION     DATGKE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310410"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VKU83_02760"
     CDS             complement(<1..319)
                     /locus_tag="VKU83_02760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLI92497.1"
                     /translation="MDTLVAAPSAIPALTGPELERYSSITSRFFDSMLAGRFNGSVLV
                     AKNGVVVYERYKGYTNPRLKKDSITEHTAFHLASMTKTFTAMATLKLWQDGKLDIHDP
                     VAKY"
     gene            510..1748
                     /gene="serA"
                     /locus_tag="VKU83_02765"
     CDS             510..1748
                     /gene="serA"
                     /locus_tag="VKU83_02765"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLI92498.1"
                     /translation="MADKKTTSYPKEKINILFLENISDAAVKHFVSSGYASVRKLSGA
                     LSEEQLINEIKDVHLLGIRSKTKITARVLDAARKLQAIGCFCIGVNQVDLRSATSHGI
                     VVFNAPYSNTRSVAELVIALSIMLIRRIPDKTKAAHEGVWMKESKGSYEIRGKTLGII
                     GYGNIGAQVSVLAEALGMKVIYCDTVTKLPLGTASARKTLKEVVSEADIVTLHVPETN
                     QTKNMINKSVLKNFKKGSILINYARGEVVDLEGLAKCLQEGALSGAAIDVFPWEPEKN
                     GDRFSTPLQGLPNVILTPHIGGSTEEAQANIGEDVSNKLFQYLETGMTLGSHTIPPLV
                     LPHQEGTHRILHIHKNVPGVLSEINSTLSQHNINILGQYLKTNDEIGYVVLDIDKRLS
                     NQAVQLLKAVRATIKTRLLY"
     gene            complement(1775..2287)
                     /locus_tag="VKU83_02770"
     CDS             complement(1775..2287)
                     /locus_tag="VKU83_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92499.1"
                     /translation="MDLLKLLRHEHSKRQTDMIVEWVGADKERFGRLIELFFSGEYRI
                     TQRAAWPLSYCVQRYPSLIVPYYKRLLDNLARTDIHVAVIRNTVRLLQHVSIPKRFHG
                     RVMNTCFDLIQKPETPIAVKAFSLTILSNLAADYPEIRGELRLIIEEQWEQATPAFRS
                     RARKILKTIK"
     gene            2334..3323
                     /gene="mgrA"
                     /locus_tag="VKU83_02775"
     CDS             2334..3323
                     /gene="mgrA"
                     /locus_tag="VKU83_02775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HLI92500.1"
                     /translation="MSYCPDPKRYDAMEYRRCGRSGVLLPAVSLGLWHNFGHVDVLEN
                     SRAILRLAFDAGITHFDLANNYGPPPGSAEENFGRILKTDFYGYRDQLIISTKAGYYM
                     WQGPYGEWGSKKYLVASLDQSLKRMGLDYVDIFYHHRPDPNTPLEETMTALDLIVRQG
                     KALYIGISNYGTEEAAKAIAILRKLGTPCLIHQPKYSMFERWVEGGLLDLLEKEGIGC
                     IPFSPLAQGLLTDKYLKGIPADSRAAKPTGFLQESQVTEDRISKVRRLNELAVKRDQT
                     LAQMALAWILKDKRVTSVLIGVSRPEQLTDSLQCFRNTWFSTEELRAIEGILK"
     gene            3455..3937
                     /locus_tag="VKU83_02780"
     CDS             3455..3937
                     /locus_tag="VKU83_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92501.1"
                     /translation="MKQLITFVALLFTAFASLANDNDGKVSSQAASSSAASTKVMNAG
                     AVRFQFNNIDAQNHADSMLVIFDRFDHTGAGVIYQVFAADQKNGFDISAIPAGKYYVT
                     IQCMGLHHDQIQKTITVKRNKNEKIKIALSATELFSKDNVVIPTTYTKLSDLSILNNK
                     "
     gene            complement(3946..>4374)
                     /locus_tag="VKU83_02785"
     CDS             complement(3946..>4374)
                     /locus_tag="VKU83_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92502.1"
                     /translation="LRLAGDLLLIYSLLGILWPFAPMHQRPVLAAGGATLSDTFHIAL
                     GAITEIIYLVVMGLVAAALGRAFRLYSIASFTVLLVFGILTFSAASGVGTNQPTPLIG
                     VWERVNIGAFLLWVSVLAIALEKRTTNDVAVRKRENVQPR"
BASE COUNT         1118 a         1156 c         1089 g         1011 t
ORIGIN      
        1 ggtatttggc gacagggtcg tgaatgtcca gctttccatc ctgccaaagc tttaatgtgg
       61 ccattgccgt aaaggtcttg gtcatcgaag cgagatgaaa ggccgtatgt tcggtaattg
      121 aatctttttt caaccgcgga ttggtatacc ctttatagcg ttcataaacg acgacgccgt
      181 tcttggccac caacacgctc ccgttgaacc gtcccgccag catcgaatca aaaaacctgc
      241 tggttatcga agaataccgt tccaattccg gtcccgtgag ggcagggatt gccgatggag
      301 ccgcgaccag tgtatccaca ggcgaagctt cctttgcggt ttgagcatga ccacaggata
      361 caaccaacaa tgttgcgcac agcgccgaaa aaccaagacc tttcaactgc gtgacttaat
      421 ttgggttaga aaaggtgcaa aattaaaata atccgccgtt actttgcatt tccatatcgg
      481 aaaagtaacg caaaaagcat ggcaaaatta tggcggacaa gaaaactacc agctacccta
      541 aagaaaagat taacatactg ttcttagaga atatctccga tgcggctgtg aaacattttg
      601 tcagctcggg gtatgcctcc gtccggaagc tgagcggcgc cctttccgag gagcaattga
      661 tcaatgaaat caaggacgtc cacctcctag gtatccggtc aaaaacgaag ataaccgcca
      721 gggtcctgga tgctgccagg aaattgcagg ccatcgggtg tttttgcatc ggcgtcaacc
      781 aggtcgacct cagatccgca accagccacg ggatcgtggt ctttaacgct ccctattcca
      841 atacgcgctc cgtagccgaa ttggtcattg cgttgtcgat tatgctgatc cgccgcattc
      901 ccgacaagac aaaggcggcg cacgaaggtg tttggatgaa agagtccaaa ggcagctacg
      961 agatcagagg taaaacattg ggtatcatag gctacggaaa tattggtgcg caggttagcg
     1021 tgctggctga agcactggga atgaaagtga tctactgtga taccgtcacg aagcttcctc
     1081 tcggcactgc gtcggcgcgc aaaacgctca aagaggtggt cagcgaggcc gatatcgtca
     1141 ccctgcatgt tccggagacc aatcaaacga agaacatgat caacaagtcc gtcctcaaga
     1201 actttaagaa aggctctatt ctcatcaact acgcccgtgg cgaagtagtc gacctcgagg
     1261 ggttggcgaa atgcctgcag gaaggggcgc tttccggcgc ggcgatcgac gttttcccct
     1321 gggagcctga aaagaacgga gaccggttct cgaccccact gcaaggtctg ccgaatgtca
     1381 tccttacccc ccatatcggc ggctcgacag aagaagcgca ggccaatatc ggggaggacg
     1441 tcagcaataa gcttttccag tacctcgaga ccggcatgac cctgggcagc cataccattc
     1501 cgccgcttgt ccttccgcat caggaaggca cccaccggat actccatata cataagaatg
     1561 taccgggtgt gctctccgag ataaatagta cgctttccca acataatatc aatatccttg
     1621 gccaatattt gaagaccaac gacgagatag gctatgtggt cttggacatt gacaaacggc
     1681 tgtccaacca ggcggtgcag ttgcttaaag cggtgcgggc gaccattaag acccgcttgc
     1741 tgtattaaac caccgatttg gcaactgaaa cgggctactt aatcgtcttc aaaatctttc
     1801 ttgccctcga tcggaatgca ggtgtagcct gctcccactg ctcttcgata atcaaccgca
     1861 actcgccccg gatctccggg taatctgcgg ccaggttcga aagaatggtc aatgaaaagg
     1921 ccttgacggc gattggcgtt tccggctttt gaataagatc aaagcaggtg ttcatgactc
     1981 gtccgtgaaa acgcttcgga atgctcacgt gctgcaacag ccgaacggtg tttcggatca
     2041 ccgccacatg aatatcggtc ctggcgaggt tatcgaggag ccgcttgtaa tagggcacga
     2101 tcaatgacgg atatcgctgc acgcaatagc ttaacggcca ggccgcacgc tgcgtgatcc
     2161 ggtactcgcc actgaagaat aattcaatca atcgcccaaa ccgttccttg tcggccccga
     2221 cccattcgac gatcatgtcg gtctgccgtt ttgaatgttc gtggcgcaat aattttaaca
     2281 ggtccatggg ttcgcctaaa gttacctaat tttataccct aattcccttg ataatgagct
     2341 attgtcccga tccaaaacgc tatgatgcca tggaataccg tcgttgcggc cgcagcggtg
     2401 ttctcttacc ggcagtttcc ctcggtcttt ggcataattt cggtcacgtc gatgtgttag
     2461 agaactcacg ggccatcctg agactggcct ttgatgcggg gatcacccat ttcgatcttg
     2521 ccaacaatta tgggccgcct ccgggttcgg cggaggaaaa tttcggtcgc atcctgaaga
     2581 ccgattttta cggataccgg gatcaactga ttatttcaac caaagccggc tactacatgt
     2641 ggcagggtcc atatggcgaa tggggctcga agaaatacct ggtggccagc ctcgaccaaa
     2701 gtctcaagag aatggggctg gattatgtcg acatttttta tcaccatcga ccggacccga
     2761 atactccttt ggaagaaacg atgaccgccc tcgatcttat tgtccgccag ggtaaggcat
     2821 tatacatcgg gatttccaat tatgggacgg aagaagcggc aaaggcaatc gccattctgc
     2881 gaaaactcgg taccccttgc ctgatccacc agccgaaata ttccatgttt gagcgctggg
     2941 tggagggagg tctgctggac ctcctggaaa aagaagggat tggatgcata cccttttctc
     3001 ctttggcgca gggattattg actgacaagt acttaaaggg tattcccgcg gattccaggg
     3061 cggccaaacc aactgggttt cttcaggagt cccaggtcac cgaagacagg atcagcaagg
     3121 tccgccggct taatgagttg gccgtcaagc gggatcagac actcgcccaa atggcccttg
     3181 cctggatcct gaaggataag cgcgtaactt cggtgctgat cggggtgagc aggccggagc
     3241 agttgaccga ttccctccag tgcttcagaa acacttggtt ttcaacggaa gagcttcggg
     3301 caatcgaggg cattctaaag taatcttatt cctggaataa gtgttcccat ctagggaaag
     3361 ctaagcgcgc attagctgcg gcaacgatct gctttccaat gttttatggt ctggcattcc
     3421 gattgtccat attatggcat aatcgtaccg catcatgaaa cagttgatta ctttcgttgc
     3481 cctattattc accgcgtttg cctccttagc gaacgacaat gatggcaagg tgtcctcgca
     3541 ggccgcctct tcctcagccg cttccaccaa agtgatgaat gccggcgccg tccgtttcca
     3601 attcaacaac atcgacgccc agaatcatgc cgattccatg ctcgtcatct tcgaccggtt
     3661 cgaccacacc ggcgccgggg taatttatca ggtttttgcc gccgaccaga aaaatgggtt
     3721 tgatatttcc gctattcctg ccggtaaata ctacgtcacg atccagtgca tgggtcttca
     3781 ccatgaccag atccagaaga cgattaccgt gaagaggaat aagaacgaaa agatcaagat
     3841 cgccctgtct gccaccgaat tgttctcgaa agacaatgtt gtgatcccga cgacctatac
     3901 caagttatcc gatctgtcga tcttgaacaa caagtaagct ccaatttacc gcggttgcac
     3961 gttttcccgc ttccttacag caacatcatt ggtcgttcgt ttctcaaggg ctatcgccaa
     4021 tacgcttacc catagcagga acgcaccaat attgacccgt tcccaaacgc caataagggg
     4081 cgtgggttga tttgttccaa ctccggatgc cgccgaaaaa gtcaaaatgc cgaatacaag
     4141 caacactgta aagctagcga tcgaataaag ccgaaaggcc cttcccagcg cagctgccac
     4201 taagcccatc acgacgaggt agatgatctc cgtaatggcg cctaaagcaa tgtggaacgt
     4261 atcggacagt gtcgcgcctc ccgccgccag gaccggccgc tgatgcattg gcgcaaatgg
     4321 ccaaagaatg ccaagtaaac tataaatgag gagcagatcg ccagccaaac gtag
//
LOCUS       DATGKE010000160         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_686892, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000160 DATGKE010000000
VERSION     DATGKE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686892"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1386
                     /gene="sucC"
                     /locus_tag="VKU83_02790"
     CDS             175..1386
                     /gene="sucC"
                     /locus_tag="VKU83_02790"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HLI92503.1"
                     /translation="MNLHEYQAKELLKKYQVPVQEGIPVDNAGAAEEAYKHLKVQFGN
                     NFAVVKAQIHAGGRGKGKIQGTEQRGVAVGKSAEEIKQIASRILGGTLVTIQTGPAGR
                     KVNKVLIAQDVYYPGANPVKEFYLSVLLDRAKGQNVIMYSTEGGMDIEEVAHKTPEKI
                     FKEWVHPCGALQPFQARKIAFNLGLSGEAFKNCVRFVTNLYNAYLGLDCSMLEINPLF
                     KTSDEKVIAVDCKMNLDDNALMRHPDLEALRDIGEEDPTEVEAGKYNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFRIILKDPKVKAILINI
                     FGGIVRCDRVAQGVIDAYESIGDIKVPIIVRLQGTNAEEAGELIRQSGLKVQSAILLS
                     EAAALVNKAVS"
     gene            1386..2213
                     /locus_tag="VKU83_02795"
     CDS             1386..2213
                     /locus_tag="VKU83_02795"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HLI92504.1"
                     /translation="MDQPAILQQINEAVGAIRAVFAVKPRIGIVLGSGLGNLVREMEV
                     VREINYESIPHFPVSTVEGHCGKLIFGLLNRQPVVVLSGRFHYYEGYTPQQVVFPIRV
                     LKFLGVEYLLVSNAAGGMNAGFRVGDLMIIRDHISLFVVNPLLGKNEGGLGPRFPDMS
                     EPYNRELIRKAKAVAERLGIPVREGVYAGVTGPTFETRAEYRLLHIAGGDAVGMSTVQ
                     EVIAARHMGMAVFAISVITDLGIREEDNLITHEEVLAAAAAAEPRLAAIFKSLVSEL"
     gene            2278..>3440
                     /locus_tag="VKU83_02800"
     CDS             2278..>3440
                     /locus_tag="VKU83_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HLI92505.1"
                     /translation="MITDGRVMSNLSRINAIVNRWRIIRAVIWMAVLLSGTAVMAQNP
                     LQRFGGMGGGGGKAADSLEHRKPDTITIHFRYLDSSRLNNLDSGILDFGKKIPRPDNW
                     INLGNLGTPARSLAFTPRMQSGFDPGFHSLDIYEYTVAAATFYNTTKPYTELGYFLAS
                     KAEQFISVFHTQNIKPNWNFVFQYRLINAPGTFKNENSNHNVFRVTSWYQSHNKRYQN
                     FMVFVANKLEVSENGGLQNPTDLDSAAYSNQQILPVKLGNNILASSGSPFNVKISTGT
                     KYSTLTFLLRQDYDLGQKDSIVTDTSVIPLFYPRLRLEHNFSYTSYNYQYMDDSLPGY
                     SLNSAYYANYGLGYVGQPQIGVGVVADTFHRQDIWHVLSNDFSIYQFPDARNP"
BASE COUNT          819 a          821 c         1045 g          755 t
ORIGIN      
        1 cggcagccga tcgaagcgat cgggcagggg gcgatggccg cattgatcga tcgcctgaag
       61 catcccgcga gcgccgaggg tttcccgccg gcggagccgc tgaagctgga gccggaattg
      121 attgcgaggg aatctatttg aaatgcccta tttttgagcc aaatttttcg atccatgaac
      181 ttgcatgaat atcaggccaa ggaacttctg aaaaaatacc aggtacccgt acaggaaggc
      241 attcccgtgg ataacgcggg ggctgccgag gaagcttaca agcatttgaa ggttcagttc
      301 gggaacaatt ttgcggtagt taaggcacag atccatgcag gcggccgggg aaaaggtaag
      361 atccagggta cggagcaacg gggagtagcg gtcgggaaga gcgcagagga aatcaaacaa
      421 atcgccagca ggatactggg cggcacgctg gtgacgatcc agacaggccc ggcaggaagg
      481 aaggtaaata aggtgctgat cgcgcaggac gtttactacc cgggggctaa tccggtaaag
      541 gagttttatt tgtccgtgct gttggaccgc gccaagggtc agaatgtgat catgtacagt
      601 accgaagggg gaatggatat tgaagaggtc gcgcacaaga cgccggagaa aatcttcaag
      661 gaatgggttc acccctgcgg ggcgcttcaa ccgttccagg cgagaaagat cgcgttcaac
      721 ctgggtctga gcggtgaggc tttcaagaac tgcgtccggt tcgttaccaa tttatacaat
      781 gcctatttag ggctcgactg ctcgatgctg gaaatcaatc cgctcttcaa gacgagcgat
      841 gaaaaagtga ttgcggtcga ttgtaagatg aacctggatg acaacgccct catgcggcat
      901 cccgacctcg aggccttgcg agacatcggt gaagaggacc cgaccgaggt ggaagccgga
      961 aaatacaacc tgaattttgt gaagctggac gggaatgtgg gctgcatggt caatggcgct
     1021 ggactggcga tggcaaccat ggacatgatc aaattgagcg gcggagagcc cgcgaatttc
     1081 cttgacgttg gtgggacggc caatgcacag acggtcgagg cgggattccg gatcattctg
     1141 aaggacccca aggtaaaggc catcctcatt aatatttttg gcggtattgt ccgttgtgac
     1201 agggtggctc aaggcgtgat cgacgcttat gaatctatcg gtgatattaa agttcccatt
     1261 atcgtcaggc tgcagggcac caacgcagaa gaggcgggag aactgatccg gcagagcggt
     1321 ctcaaggtgc agtcggccat attgctgagt gaagcggccg ctttggtcaa taaagcggtg
     1381 tcctgatgga tcagccggcg atcttacagc aaataaatga agcggtaggc gccatccggg
     1441 cggtttttgc ggttaaaccc cggatcggga tcgtgctcgg cagcggcctg ggtaaccttg
     1501 tccgggagat ggaggtggtg cgcgagatca actatgaatc gattcctcat ttccccgtct
     1561 cgaccgtcga gggtcactgc ggtaaattga tcttcgggct attgaaccgt cagccggtag
     1621 tagtcttatc gggccgtttc cattactacg aggggtatac gccgcagcag gtggtcttcc
     1681 cgatccgggt attgaaattc ctcggagtcg aatacctgct cgtgtcgaat gcggccggcg
     1741 ggatgaatgc cgggtttagg gtgggggatc tgatgatcat ccgggatcat atcagtctct
     1801 tcgtggtgaa tccgctcttg ggaaaaaatg aaggcgggct gggcccgcgg ttcccggata
     1861 tgagtgagcc atataacagg gaattgatcc ggaaggcaaa ggcggtggcg gaacgattgg
     1921 gaattccggt gcgtgaaggg gtttatgcgg gcgttacggg gcctaccttc gagacccgcg
     1981 cggaatatag gttattacat attgcgggcg gcgatgcggt gggcatgagt acggtgcagg
     2041 aggtgatcgc ggcccggcac atggggatgg cagtgtttgc gatcagtgtg atcaccgacc
     2101 tcgggataag agaggaggac aacctgatca cccatgaaga ggtgctggcc gccgcagccg
     2161 cggcggagcc gcggctggcg gcgatattca agtcccttgt ttcggaactg tgagcggcat
     2221 tgacaggagg tccgccgcgg caaagctatg gcgggcgagg caacattaac gttttttatg
     2281 atcaccgatg gcagggtaat gagtaacttg tcccgaataa atgcgatcgt gaaccgttgg
     2341 cgcatcatta gagccgtgat ctggatggcc gtacttcttt cgggaacagc ggtgatggcg
     2401 cagaatccct tgcagcggtt cggcggtatg ggcgggggcg gtggaaaggc agcggactcg
     2461 ctggaacacc ggaagccgga tactatcacg attcactttc gttacctgga cagcagccgg
     2521 ctgaacaacc tcgattccgg tatcctggac ttcggtaaaa agatcccgcg gccggataat
     2581 tggatcaatc ttggcaattt ggggacaccg gcgcgcagcc tggcttttac cccgcggatg
     2641 caatcgggtt ttgaccccgg attccattcc ctcgatattt atgaatatac ggtggccgcg
     2701 gcgacgttct acaacaccac caagccctat accgagctgg gctatttcct ggctagcaaa
     2761 gcggagcaat tcatcagcgt ctttcatacc caaaatatca agccgaactg gaatttcgtg
     2821 ttccaatacc ggttgatcaa tgctccgggc actttcaaga acgaaaacag caatcacaac
     2881 gtattccgtg tcacctcctg gtaccaatcg cataacaagc ggtatcagaa ttttatggta
     2941 tttgtggcga acaaactgga agtgtcggaa aacggcggac tgcagaaccc gacagacctg
     3001 gattcggcgg cttacagtaa tcaacagatc ttgccggtga aattggggaa taatatcctg
     3061 gccagttcgg gtagcccgtt caatgttaag atcagtaccg gaaccaaata ctctactttg
     3121 acctttcttt tacggcagga ttacgacctg ggacagaagg attcgatcgt gacggacacc
     3181 tctgtgatcc cattgtttta tccgcgcctg cggctggagc acaacttcag ttatacttca
     3241 tacaattatc agtacatgga tgacagcctg ccgggttatt cgctgaattc ggcttactac
     3301 gccaactacg ggttaggata tgtgggccag ccgcagatcg gcgtaggagt ggtggcagat
     3361 accttccacc gtcaggatat ctggcatgtt ctatccaatg acttttcgat ctatcaattt
     3421 cccgacgccc ggaacccgca
//
LOCUS       DATGKE010000161         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_996909, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000161 DATGKE010000000
VERSION     DATGKE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996909"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="dusB"
                     /locus_tag="VKU83_02805"
     CDS             complement(<1..935)
                     /gene="dusB"
                     /locus_tag="VKU83_02805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HLI92506.1"
                     /translation="MEDVSDPPFRMICRAAGADLTYTEFINSEGLLRGTASSRRKLDF
                     DETERPVGIQLYGGDEACLAKAAAIVDATRPDLLDINFGCPVRGVVGRGAGAGVLRDP
                     DRMVRLTAACVRSTALPVTVKTRLGWDDDSKNIETIAERLQDAGIRALTIHGRTRCQL
                     YKGEADWTLIGKVKANPRIRIPIFGNGDIDSPQRAVAYRNRYGIDGIMIGRAAIGHPW
                     IFREIRHFASTGEIAPSPTVQERVAVCIAHLEGAIGWNGPKQGLYEMRGRYARYLKGL
                     PGISEWLHEMVRAPDPAAARQLLHEIGKAYRGYEIA"
     gene            1121..1612
                     /locus_tag="VKU83_02810"
     CDS             1121..1612
                     /locus_tag="VKU83_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92507.1"
                     /translation="MKIPFNRALSLLLAGIAILLGAGNCKKSSNNGNAALSASVSGKD
                     WTANYPVRGTYLGGQFEIGGLRVKGGDSTVLGLIFTAPVVLNKPVSSDTSQIDVASVD
                     NETKTEYDGSTPGHSIITIISYDSASRKIAGTFTGVLYNDTNGADSLIVTGGAFSASY
                     TVQ"
     gene            2009..2521
                     /locus_tag="VKU83_02815"
     CDS             2009..2521
                     /locus_tag="VKU83_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92508.1"
                     /translation="MRFPFNRAMAFLVLSSAVVFAAASCKKSGTGNSGATLSASVSGT
                     AWTPNYPTAAYYATSGSQFNIVGLYYKNGDSTAFVLDFFSPVNVNQAINSDTSNIDVQ
                     YVVAKPGSTQVYDGSYMSGGHAILTVTSYDSTGHKIAGTFSGVLYNVSGGTDSVLVTN
                     GAFNTSYSVQ"
     gene            complement(2565..>3197)
                     /locus_tag="VKU83_02820"
     CDS             complement(2565..>3197)
                     /locus_tag="VKU83_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HLI92509.1"
                     /translation="VLSTDGQGFIEGNAWNYTLYVPEDPDGLIALLGGKKRFVQRLDS
                     LFTMTLPDKFFAETEDITRDGIIGGYVHGNEPSHHVAYLYDWTDQPWKTQERVRMILD
                     NQYHSGPAGLGGNDDTGQMSAWYIFTALGFYPVAPGSDRYWLGSPAIDKATLRLENGH
                     SFRIETRNQGAKNVYVRKVTLNGQVLNRRYITHEEIMNGGLLVFDMSAKH"
BASE COUNT          726 a          910 c          831 g          730 t
ORIGIN      
        1 gcgatctcgt atccccggta ggccttgccg atctcatgca agagttgccg tgccgcggca
       61 ggatcgggcg ccctgaccat ttcgtgcagc cattcgctga tgcccggcaa ccccttcaga
      121 taacgggcat aacggccgcg catctcgtac agtccttgtt tcgggccatt ccagccaatc
      181 gctccttcga ggtgagcgat gcagacggcg acgcgttcct gaacggttgg cgatggcgcg
      241 atctcccccg tcgaggcgaa atgtcggatc tcccggaaga tccagggatg gccgatcgcg
      301 gcgcggccga tcatgatgcc gtcgattcca taccggttcc ggtacgccac cgctctttgc
      361 gggctgtcaa tatcgccatt gccaaagatc gggatgcgga ttcgcggatt ggccttcacc
      421 tttccgatca gggtccagtc ggcttcgcct ttgtataact gacatcgggt ccggccgtgg
      481 atcgtaaggg cccggatgcc tgcatcctgg agccgttcgg cgatggtctc tatattcttg
      541 ctgtcgtcat cccagccaag ccgggtcttt accgttaccg gtagtgctgt ggaccggacg
      601 caggcggcgg tcagccgaac catgcggtcc ggatccctca aaacgcccgc gcctgctccc
      661 cggccgacca ctcccctgac cggacaaccg aagttgatgt ccagaagatc gggccgggtg
      721 gcatcgacga tcgccgcggc ttttgccagg caggcttcat ccccgccata cagttggatc
      781 ccaacaggcc tttccgtttc atcgaaatca agcttgcggc ggctactcgc cgttcccctg
      841 agcagcccct cgctgttgat gaattctgta tatgtcaggt cggccccggc agcgcgacaa
      901 atcatgcgaa aaggcgggtc gctgacgtcc tccatcggcg ccagcagaag cggaaaatcg
      961 ggcagggaaa tggaacctat ctggaccagc tgttcttgta ttaaagattt tgataatctc
     1021 gttgtaaaat taaggcaaaa tgggtgaacc gtctcaataa agaacagttc caatcgttta
     1081 tagaagagaa gctgtcatgc ttagcttcta gaaaacgtcc atgaaaatcc cctttaaccg
     1141 cgcgctttcc cttttactcg caggcattgc cattctcctg ggcgcaggaa actgtaaaaa
     1201 aagcagcaat aacggaaacg ctgccttgtc tgcctccgtg agcggcaagg actggactgc
     1261 gaattacccg gtcaggggaa cttacctcgg cggccaattt gaaatcggtg gccttcgggt
     1321 caaaggcgga gactcgacgg ttctcggctt gatattcacc gcgccggtcg tccttaataa
     1381 gccggttagt tccgacacct cacagatcga cgtcgcttcc gtggacaacg aaacgaagac
     1441 cgaatacgac ggttctaccc cagggcactc catcatcacc atcatcagtt atgactctgc
     1501 gagccgaaag attgccggaa ccttcacggg cgtactgtat aatgatacca acggggctga
     1561 cagcctcatt gtcaccggcg gcgctttttc cgcaagctat accgtgcaat agatccatcg
     1621 tcaaccgctt ttcggaaaac gaaaaaggtc aggcaacgcc tgacctcaat ctcaaatttc
     1681 tttagtgagg cgcttatctg cctacggtgc tgttaatgaa gagatacgac aagaagagaa
     1741 cgagactcag gaaagaaatg gcaagtacga tggtaatcgc agtattgccc ggatggatag
     1801 ggttgtgttt catatgacag atttttaatg aataaataaa atcgtcatac acaatatata
     1861 taaaaaaaat cgcatttgca agcccggtaa gagacaggtt acccgtccgt tatgacagca
     1921 gccggcaata aacggatcgc cgtcgccgtt tacagcaggt aaacagtcct ttctaatatc
     1981 ctcctaacaa ctaaaatcct tattggctat gagatttcct tttaaccgcg ccatggcttt
     2041 cctggttctg tcatcagcag ttgtattcgc cgccgccagc tgcaagaaaa gcggcacggg
     2101 caactccggc gcaacattga gtgcttctgt gagcggcact gcctggacgc ccaactatcc
     2161 caccgccgcc tattatgcta ccagtggcag ccaattcaat atcgtcggcc tctattataa
     2221 aaacggcgat tcaaccgctt ttgtcctgga cttcttctct cctgtcaacg tgaatcaggc
     2281 catcaactcg gacacttcaa acatcgacgt gcaatacgtt gtcgcgaaac ccgggtcgac
     2341 acaagtatat gacggatcgt acatgtcggg tgggcacgcg attttgacag tgacgtcgta
     2401 tgattccacc ggccacaaga tcgctgggac gttcagcgga gtactctaca acgtgtccgg
     2461 cggtaccgac agcgtgctgg tcacgaacgg cgccttcaat acctcctact ccgtacaata
     2521 gcttaccttc tttcgataat gagaaaggtc agggcccatc gccgttaatg tttggcgctc
     2581 atgtcgaata cgagaaggcc gccattcatg atttcctcat gcgtaatgta gcgccggttc
     2641 aggacctggc cattcagggt gactttgcgg acataaacat tttttgcacc ctggttcctg
     2701 gtctcgatcc gaaagctgtg gccgttttcc agccggagtg tcgccttgtc aatggcgggg
     2761 ctgcccagcc agtaacggtc ggacccgggc gccaccggat aaaagcccag agccgtaaaa
     2821 atgtaccagg cgctcatctg gccggtgtca tcattgccgc ccaggccggc gggaccggaa
     2881 tgatattggt tgtcgaggat catcctgacc ctctcctgcg ttttccacgg ctgatccgtc
     2941 cagtcataga gataggctac atggtgggaa ggctcgttcc cgtggacata accgccgatg
     3001 atcccgtccc gggtgatgtc ttcggtttcc gcaaagaact tgtcgggcag ggtcatcgtg
     3061 aacagcgagt ccagccgttg aacaaatctc ttttttccgc cgagaagtgc gatcaacccg
     3121 tcaggatcct ccgggacata gagggtgtag ttccaggcat tcccttcaat aaacccctgg
     3181 ccatcggtgc tcagaac
//
LOCUS       DATGKE010000162         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_65582, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000162 DATGKE010000000
VERSION     DATGKE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65582"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VKU83_02825"
     CDS             complement(<1..1063)
                     /locus_tag="VKU83_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HLI92510.1"
                     /translation="MKAGLTKRKMTFDEHSSDGRSFVSFPITEFLPIKRQMLTWASRV
                     GIFCFLDSQDFPAQIPPGLDCLLAAGAAEFLEAPAGNAFQQLRDWAEGRRDWIFGHFA
                     YDLAAETEPPPTGAAPQCRPDPVGFPDLFFFIPQVVLRLSRDSISIGSLNADQEAIWN
                     EIRCSCAEPLYAGHARPGFQDVPAFKAGLTRKAYLDRLADLQRHIHRGDCYEINFCQE
                     FYSQPAELDPIAAWWRLGEASPNPFSCLYRLDERWLLCASPERYLKKTGDRVWSQPMK
                     GTIARHPGRPEADEAARVGLLQSEKDRSENVMVVDLVRNDLARVCVPGSVAVAELYGV
                     YPFPQVFQMVSTISGILAPG"
     gene            complement(1026..1484)
                     /locus_tag="VKU83_02830"
     CDS             complement(1026..1484)
                     /locus_tag="VKU83_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92511.1"
                     /translation="MAPKSDKGGKGSKKSAPQAPKDTPEKPSSKARKQLDDDDDDDVD
                     DDDDAPKASAKSSPKGSSKTKKAEEDDDDDEDDFEEVDEWEKVEEEEEWDPDFDEFDI
                     PKSKTKKSTGGNTSKKSAKGADEDDLGLDDDFKDLDLFNESGFDEEEDDF"
     gene            complement(1566..2831)
                     /locus_tag="VKU83_02835"
     CDS             complement(1566..2831)
                     /locus_tag="VKU83_02835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLI92512.1"
                     /translation="MVDVILGLQWGDEGKGKIVDYFAPRYDLIARFQGGPNAGHTLYV
                     KDKKIVLHQIPSGIFHESTMNLIGNGVVLDPVTLKRECETVASFGVDYRKNLYIAQRA
                     HLILPTHRALDKASEMSKGQEKIGSTLKGIGPAYMDKTGRNGLRVGDLLDKTFTTQYI
                     KLRLKHQKLLDSLHFQEELSEWEEQFFEALDFMRQFKIVNGEYFINDQLSRGKTVLAE
                     GAQGSMLDVDFGTFPFVTSSNTISAGVCTGLGVAPQRIRDVIGVSKAYCTRVGSGPFP
                     TELEDATGEALRKTGNEFGATTGRPRRCGWIDGVALKFACMINGVTKIVMTKADVLDA
                     FPELKVCTAYSIDGAQTAEIPFQMTRLQIEPVLQTFAGWKSDITGVKTFAGLPQKMKE
                     YVQFINSYLGVGIAYISNGPGRDQLIEAH"
     gene            complement(2909..>3228)
                     /locus_tag="VKU83_02840"
     CDS             complement(2909..>3228)
                     /locus_tag="VKU83_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLI92513.1"
                     /translation="AAKLMANYGHRVVKTKWAKNKEISFLTGVRIVGMDDVGVINKIT
                     NLISGELRLNINALTIEAKDGLFEGNIRIYVHDKEELEELVSRLLQLNGIHSVVRYDA
                     DTA"
BASE COUNT          659 a          941 c          853 g          775 t
ORIGIN      
        1 ggcccggggc aaggatcccg ctgatggtag agaccatctg gaatacctgg ggaaacggat
       61 acacgccata aagctcggcg acggccacgg agcccggaac gcaaaccctc gcgaggtcat
      121 tgcgcaccag atcgacgacc atgacattct ccgacctgtc cttttcgctt tgaagaagcc
      181 cgacacgtgc cgcctcatcg gcttccggcc ggcccgggtg ccgggctatg gtgcccttca
      241 tcggttggga ccaaaccctg tcccccgtct tcttcagata acgctccgga ctggcacaaa
      301 gcagccagcg ttcatccagg cggtaaaggc aggaaaaagg atttggcgag gcctccccta
      361 gccgccacca tgcggctatc gggtccagtt ctgccggctg ggagtaaaac tcctggcaga
      421 aattgatctc ataacaatct ccgcggtgga tatgccgctg gaggtccgcc agccggtcca
      481 ggtacgcttt cctggtcagg cctgccttga acgccgggac atcctggaaa ccgggcctgg
      541 cgtgaccggc gtaaaggggc tccgcacagc tgcaccggat ttcgttccag atcgcctcct
      601 ggtcggcgtt caaagacccg atgctgatgc tgtcccggct caaccggagc accacctgcg
      661 gaataaagaa aaaaaggtcc ggaaagccta ctgggtcggg gcggcactgc ggcgcggcgc
      721 ccgtgggcgg cggctcggtt tccgcggcaa ggtcgtacgc aaaatggcca aagatccagt
      781 cccgcctgcc ctccgcccaa tcccttagct gctgaaacgc attccctgcc ggggcttcga
      841 ggaattccgc cgctcctgcg gccagcaggc aatccaaccc gggcgggatt tgcgctggaa
      901 agtcctggct gtccaggaaa caaaaaatgc cgacgcggga ggcccaggtc agcatttgtc
      961 gtttgatcgg gagaaattcg gttatcggga acgagacgaa ggatcgtccg tcggacgaat
     1021 gctcatcaaa agtcatcttc ctcttcgtca aacccgcttt cattgaaaag atccagatct
     1081 ttgaagtcgt catctaaacc cagatcgtcc tcgtcggcgc cctttgccga tttcttggaa
     1141 gtatttccgc ccgtgctctt ttttgttttt gattttggga tgtcgaactc gtcaaaatcg
     1201 ggatcccatt cctcctcctc ttcgactttt tcccactcat ccacctcctc aaagtcgtcc
     1261 tcgtcatcat catcgtcttc ttcagccttc tttgttttcg atgagccctt gggcgacgat
     1321 ttcgcggaag ccttgggagc atcatcatcg tcatccacat catcatcatc atcgtcatcc
     1381 agttgtttcc tggctttaga ggacggtttt tccggcgtgt cctttggtgc ctgaggcgcg
     1441 gatttctttg aaccctttcc ccccttatca gattttggtg ccatgcccaa tgagattgta
     1501 gtgtaaattt tcggcgaagt ttttaatccg ccaaattttt ttttgtttaa ctttttgcgt
     1561 tttttttaat gggcttctat cagctgatcc cggccgggac cattggaaat ataggcaatt
     1621 ccaacgccca gatagctgtt gatgaactga acgtattcct tcattttttg gggcaacccc
     1681 gcgaatgttt taacgccggt aatatcggat ttccacccgg caaatgtttg caggaccggt
     1741 tcgatctgca accgggtcat ctggaaggga atttcggcgg tttgcgcgcc atcgatcgaa
     1801 tatgccgtac aaaccttcag ttcggggaat gcatccagga catcggcctt ggtcatgacg
     1861 atcttcgtaa cgccgttgat catgcaggca aatttcagcg cgacaccgtc gatccagccg
     1921 cagcgccggg ggcggccggt cgtcgccccg aactcattgc ccgttttgcg caacgcttct
     1981 cccgtcgcat cttccagctc ggtcgggaac ggcccgcttc cgacacgggt acaataggct
     2041 ttgctgacac ctattacatc gcggatccgc tggggggcca cgcccaggcc cgtgcaaacg
     2101 ccggcagaaa tggtattcga ggaagtcacg aacgggaagg tcccgaaatc aacgtccagc
     2161 atgctgccct gcgcgccttc ggcaaggacg gtcttacccc gggacaactg atcgttgata
     2221 aaatattcgc cgttgacgat cttgaattgc cgcataaagt ccagcgcttc gaaaaattgt
     2281 tcttcccatt ccgacagttc ctcctggaaa tggagactgt ccagcagctt ctggtgcttc
     2341 agtcgaagct tgatgtactg ggtggtgaag gtcttgtcga gaaggtcccc taccctgagg
     2401 ccgttccggc cggtcttatc catataggca gggccgatgc ccttgagcgt gctgccgatc
     2461 ttctcctgtc ccttggacat ttcagacgcc ttatccaggg cccggtgggt tggcaggatc
     2521 aggtgcgccc gctgggcgat ataaaggttc ttccggtagt caacgccaaa cgacgcaacg
     2581 gtttcgcact cccgcttcag ggtgacaggg tccagcacga cgccgttccc gatcaggttc
     2641 atggtgcttt cgtgaaaaat gccggaaggt atctgatgca gaacgatctt tttatccttg
     2701 acgtagaggg tatggccggc gttgggacca ccctggaacc gtgcgatcag gtcatagcgg
     2761 ggcgcgaaat agtccactat ctttcccttt ccttcgtctc cccactggag gcctaaaatg
     2821 acatcgacca ttaattaagc atttgacttt gaggcggcaa aaatacgcga acagccggtt
     2881 taaaaaaaaa gcattcggaa ccgtatggct acgccgtgtc cgcgtcatac ctgacgacgc
     2941 tgtggatgcc attcagctgc aggagccgcg acaccagctc ctccagttct tccttgtcgt
     3001 gcacataaat gcggatattt ccctcgaaaa gcccgtcctt ggcttcgatc gtcagcgcat
     3061 tgatatttag cctcagctcg ccggaaataa ggttggtgat cttgttgatg acgccgacat
     3121 cgtccatccc cactatccgg acgccggtga ggaaggagat ctctttgttc ttcgcccatt
     3181 ttgtcttgac cacccggtgg ccgtaattcg ccatcagctt ggcggcat
//
LOCUS       DATGKE010000163         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_168738, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000163 DATGKE010000000
VERSION     DATGKE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168738"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VKU83_02845"
     CDS             <1..1058
                     /locus_tag="VKU83_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLI92514.1"
                     /translation="FEFSPRAIIPVALACVTGAGMHYLLFGQGPVFEMPYIPEPTDTA
                     LVIYVLLGALIGVVAAGVSRSVYWIEDLFEKLPIHWMWWPAIGAIAIGVVGYFAPHTM
                     GVGYDNIKVLLTGTAPFVMILSLCFLKYLSWSISLGSGTSGGTLAPLFTIGGALGCLL
                     GGLILHWFPSSGINPATAALIGMAAMFAGASRALLTSIVFALETTGQMHGLLPLLGAC
                     VAAYFVSFFLMKGSIMTEKIRRRGVKTPDAYEPDILRTVTVRELLVPVKSEPAPARSI
                     GAAEDAGLAAELMGKYNTDALAIRDEEGAVIGMITARGLLEFYSRQRQKEQRYQSPAG
                     TRRILAHGRHIARKMRF"
     gene            1154..1591
                     /locus_tag="VKU83_02850"
     CDS             1154..1591
                     /locus_tag="VKU83_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92515.1"
                     /translation="MYKEHEQEHPSVFFKAMMTGLFVGIIDTLICLGFNIGYRYETGY
                     FPSEFINVSSVIFMVNLIMLVVGLVYFVFIRFAGGNTAYVIFMLLVTAFLAWKSATVV
                     RFGDSHMDGGFHGLLTGIVLIMGLSGACIPVLYNNEKFLDGVI"
     gene            complement(1987..>2219)
                     /locus_tag="VKU83_02855"
     CDS             complement(1987..>2219)
                     /locus_tag="VKU83_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI92516.1"
                     /translation="KDYEDKHIVLPEVDPIDAIKLKMEERGLRARDLEPLIGSKGHVS
                     SILSGRRELTLKMAQKLKDYFQLPADIFMPTA"
BASE COUNT          446 a          492 c          728 g          553 t
ORIGIN      
        1 tgttcgagtt ctcgccgcgg gcgatcatcc cggtagcgct ggcgtgtgta acaggcgcgg
       61 ggatgcatta cctgcttttc gggcaggggc cggtttttga gatgccatac attccggagc
      121 cgacggacac ggcgttggtc atctatgtac tgctcggggc cctgatcggg gtggtagcgg
      181 cgggtgtatc gcgttcggtc tattggatag aggatctatt cgaaaagctt cccattcact
      241 ggatgtggtg gccggcgata ggggccattg ccattggcgt ggtcggttat tttgcgccgc
      301 acacgatggg ggtgggttat gataacataa aggtgctatt gacggggacg gcgccgttcg
      361 tcatgatctt atcactttgt tttttgaaat atctgagctg gtcgatcagt ctggggagcg
      421 gcacatccgg cgggacgctg gcgccgttgt ttaccatagg cggggcgctt ggctgtctgc
      481 tgggcgggct gatcctgcat tggttcccgt catcggggat caatccggct acggcggcgc
      541 tgatcggcat ggcggcgatg tttgccgggg cgtcgcgggc gctgcttaca tcgatcgtct
      601 tcgcgttgga gacgacgggg cagatgcacg gcttgctgcc gttgttaggg gcctgtgtag
      661 cggcgtattt cgtgagcttc tttttgatga agggaagtat catgaccgag aagatccggc
      721 ggcggggtgt aaagaccccg gatgcgtatg agccggatat cctgcggacg gtaacggtgc
      781 gggagctgct ggtaccggtc aagagcgagc cggcgccggc acgttcgatc ggcgcagcgg
      841 aagatgcggg gctggcggcc gagctgatgg gcaagtacaa tacggacgct ttagcgatcc
      901 gggatgagga aggtgcggtg atcgggatga tcacggcgcg gggtttgttg gaattctata
      961 gccggcagcg gcaaaaagag cagcggtatc aatcgccggc cggcacgcgg cggatactgg
     1021 cgcatggaag acatattgcc cggaaaatgc gcttttgaaa aaatagcgat tttccggtca
     1081 tgcgctaaag tctgtcctgg cggacagccg ccgccggcgg ctaaggtttt acagaacgca
     1141 taaatcataa catatgtata aagaacatga gcaggagcat ccttctgtat ttttcaaggc
     1201 gatgatgacg gggttattcg tggggatcat cgatacattg atctgtctcg gtttcaatat
     1261 cggctatcgg tatgagacgg gatatttccc gtccgagttc atcaatgtga gctcggtgat
     1321 ctttatggtg aatctgatca tgctggtggt ggggttggtt tattttgtat ttatccggtt
     1381 cgccggcggc aataccgcct atgtgatctt tatgttgctg gtgacggcct ttctggcatg
     1441 gaagtctgcg acggtagtgc gtttcggcga cagccatatg gacgggggtt tccatgggct
     1501 gctgacgggt atcgtgctga tcatggggtt aagcggggcg tgtataccgg ttttgtataa
     1561 caatgagaag ttcctggacg gcgtgatcta ggggccgccg gaggcgggcg gcggcagtgg
     1621 ggatcggtgt aaataagtat ttttggtttc gacggaaacc attatattcg agttacgaaa
     1681 acactttgac ctcattataa acgatgtgtc taaattaagc caaagtgatt ccttttgcgg
     1741 ctgctatttc cataggagcc aataaggaat cgagccggcg gatgccgagg ctgatggcgc
     1801 cggccgagat gtgaggtaaa aagaaggctg gccggtgcgg ggagggctgt caaaggcgag
     1861 ggtgaggacc tgcaaagggc gggggacctg ccaaaggcga gggaccggca taggccgggc
     1921 agagatatgc gagcttccga tgattgtcgg gggctttgtt gtttttgggg cacataaagg
     1981 tatagactac gccgtaggca tgaatatatc cgcgggtagc tgaaaataat cctttagctt
     2041 ctgcgccatt ttcaatgtca gctccctccg tccggaaagg atagatgaaa cgtgaccttt
     2101 tgatcctatc aacggctcca ggtccctggc tctcagacct ctttcttcca tcttcaactt
     2161 tatcgcatca atcggatcga cctcgggcag aacgatatgc ttatcctcat aatctttta
//
LOCUS       DATGKE010000164         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1102936, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000164 DATGKE010000000
VERSION     DATGKE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102936"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VKU83_02860"
     CDS             <1..895
                     /locus_tag="VKU83_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HLI92517.1"
                     /translation="TAFGYAHLPHAGVGLAREEFIINNYIKAHPLALLQHRQLKDASL
                     SAAIQTMIRGFGDEETYFIEKLSYGVAKIAAAFYPHTVIVRFSDFKSNEYRNLLGGAY
                     FEPEEENPMIGWRGASRYYSPAYKPAFGLECRAIRYVREQLGLSNVAVMIPFCRTVKE
                     LLQVQATMAEFGLARGEAGLRLFLMAELPSNVLLAAEFAKHIDGFSIGSNDLTQLTLG
                     LDRDSALVAGLYDERDEAVKTLLRMLIRTAKAAKVKVGICGQGPSDFPEFARFLVEEG
                     IDSISVTPDSFVKTVRALADL"
     gene            962..>2589
                     /locus_tag="VKU83_02865"
     CDS             962..>2589
                     /locus_tag="VKU83_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLI92518.1"
                     /translation="MRLNLIFLLFLFSMVCRGQGYNLIPYPQVLEPGQGQFVVNAQLR
                     VVASPGFEREGVMLRRLLQKALGRALTGSGREILLEHDVGITRPEAYTLVITPRRIEI
                     KAGGSDGIFHGIETIRQLLPAAAERGKVGDPPRLPAESRKVSDSLALPAVRIEDYPAY
                     SWRGMHLDVSRHFFSIDYLRRFIDRMALYKMNKLHLHLSDDQGWRVEIKHYPLLTEAG
                     AWRAFNNQDSACMRRAADDPDFAIDSSHLIRRNGRVLYGGFYTQEQLRALVAYAGERH
                     IDIIPEIDMPGHMMAAINSYPFLTCNGENKWGELFTKPICACNETTYTFAENVFSEIM
                     DIFPSPYIHIGGDEVDRSDWAKSAACRALMERMGIKDLPGLQSYFISRMEKFFRSKGR
                     ELVGWDEIIEGPISPDALVMYWRGWVPGAPLKAARNGNKVVMAPGEPLYFDRPADQYD
                     LSNVYHFNPVGKISDTALAAKIIGAQACLWSEQLPNEKRCDYMYMPRMTALAEALWTA
                     RPEGWDTYVHRLGAHFPRMDAMGINYRLPDLPDLL"
BASE COUNT          533 a          648 c          820 g          588 t
ORIGIN      
        1 gactgctttc gggtatgccc atctgccgca tgccggcgta ggccttgcgc gcgaagagtt
       61 catcatcaac aactacatca aggcgcatcc gctggcgttg ctgcaacatc gccaactgaa
      121 ggatgcgtcg ctctccgccg ccattcagac catgatccgc ggtttcggcg atgaggaaac
      181 ctattttatt gagaagctct cttatggggt tgccaagatc gcagccgctt tttatccgca
      241 tacggtgatc gtacggttct ccgatttcaa gagcaatgaa taccggaacc tattgggcgg
      301 cgcttatttc gagccggaag aagaaaatcc gatgatcggc tggcggggtg catcgcgata
      361 ttattctccc gcttataaac ctgcctttgg gctggaatgc cgggcgatcc gctatgtacg
      421 cgagcagctc ggtttgagca acgtcgccgt gatgatcccg ttctgccgta cggtaaaaga
      481 gctgctgcag gtacaagcca ccatggcgga attcggactg gcgcgcggcg aagccgggct
      541 acgcctcttt ctaatggcgg aactgccgag caatgtgctg ctggcggcgg aattcgccaa
      601 acatatcgac ggattctcga tcggctccaa cgatctcaca caacttaccc tgggactcga
      661 ccgcgattcc gcgctggtgg cggggctata cgacgagcgg gacgaggcgg tgaagacctt
      721 gctgaggatg ctgatccgga cggccaaggc agccaaggta aaagtgggga tctgcggaca
      781 gggtccctcg gatttcccgg agttcgcccg ttttctggtt gaggaaggga tcgacagcat
      841 tagcgtgacg ccggattcgt tcgttaaaac ggtgcgagcg ttggccgatc tttgaaggcc
      901 ggggccgctc cagtggaggg gccgaaggtc aaacagacat tagccttata tttgccgcat
      961 tatgcggctg aacttaatct ttttactgtt cttgttttcg atggtttgcc gggggcaggg
     1021 ctataacctg atcccctatc cgcaggtgtt ggaaccggga caagggcaat ttgttgtaaa
     1081 tgcgcagttg cgggtggtgg cgtcgccggg tttcgagcgg gagggggtaa tgctccggcg
     1141 tctgttgcaa aaggcattgg gtcgggcatt gaccggttcg gggcgggaga tcctgttgga
     1201 acacgatgtc ggaataaccc ggccggaagc atatacgctg gtgatcactc cgcggcggat
     1261 agagatcaag gcagggggct ccgatggtat ctttcatggc atcgagacga tccggcagct
     1321 gttgccggct gcggccgaac gaggcaaagt gggtgatccg ccccgactac cggctgaaag
     1381 ccgaaaggtt agcgactccc ttgcacttcc tgccgtgcgg atcgaagact accctgccta
     1441 tagctggcgg gggatgcatc tggacgtgtc gcggcatttc ttttccatcg attatctgcg
     1501 gcggttcatc gatcgcatgg cgctttacaa aatgaataag ctgcatctcc atctttcgga
     1561 cgatcagggc tggcgtgtag agatcaagca ctatccctta ctgaccgagg cgggcgcctg
     1621 gcgggccttc aacaaccagg actctgcctg tatgcgaagg gcagccgacg atcctgactt
     1681 tgccatcgac agcagccacc tcatccggcg taacgggcgg gtcctttacg ggggttttta
     1741 tacgcaggag cagctacgtg cattggtggc gtatgcgggg gaacggcata tcgacatcat
     1801 tccggagatc gacatgccgg gccatatgat ggcggccatc aattcttacc ccttcctcac
     1861 ctgcaatggc gaaaacaaat ggggtgagct gttcaccaaa cctatatgtg cgtgcaacga
     1921 gacgacctat accttcgccg agaatgtgtt ttcggagatc atggatattt tcccttctcc
     1981 ttatatccat atcgggggcg acgaagtgga ccggtcggac tgggcaaagt cggcggcttg
     2041 cagggcgttg atggaacgga tggggatcaa ggatcttccg ggtttacaaa gctactttat
     2101 aagccggatg gagaagttct ttcgatcgaa gggccgcgag ctggtcgggt gggatgagat
     2161 catcgaagga cctatttcgc ccgatgcgct ggtcatgtat tggcggggtt gggtgccggg
     2221 cgctccgctg aaggcggcgc gaaacgggaa taaggtagtg atggcaccgg gtgagccgtt
     2281 gtattttgac cggccggcgg atcagtatga cttgtcgaat gtgtatcatt ttaatccggt
     2341 ggggaagata tccgacacgg cgttggcggc gaagatcatc ggggcgcagg cttgtttatg
     2401 gagcgagcag ctgcccaatg agaaacggtg cgattatatg tatatgccgc ggatgacggc
     2461 gttggcggag gcgttgtgga cggcgcggcc ggaagggtgg gatacttatg tacataggct
     2521 aggcgcacat tttcccagga tggatgcgat ggggatcaat taccggcttc ccgatctacc
     2581 cgatttgct
//
LOCUS       DATGKE010000165         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1102754, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000165 DATGKE010000000
VERSION     DATGKE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102754"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="VKU83_02870"
     CDS             <1..1797
                     /locus_tag="VKU83_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92519.1"
                     /translation="KELQAAAAGYQAAANQAALAGNAALAAQYQMQAQQASQLAAATP
                     SNSNVTRTGYAESALTNYRADNFKVYGGLYYKVTPGSTLSLIGNWGTASTIYQGTDRY
                     SLKDVRIGQYKLELTGQHYYVRAYTTQENAGNSYDMVAVATLMNEKYAPTATVWGPTY
                     IGAYAQSFGADLQAGQDPATAYANASAVARGAADQNRLQPGSPAFNQAFNTLKQQAIP
                     YGGKFNDQTNLYVGEAMYNFANEVKWIDITVGAATREFVLNSHGTIFADTAGRININE
                     SGAFAQLQKGFLDDLLKLSVAGRYDKNSNFKGRFTPRVTALLRVAPDNYIRASFQTAY
                     RFPSTQNQYINLQTGSARLIGGLPQFITAYGLNDGQTYDTAQLNKYMATGTPPTAYQY
                     QTFKPETVASWEVGYKGLIGKNLLLDVYGYYAQYTNFIGLTVLVKDPLTLGQTAANTF
                     GIYTNSPTKVNTGGAGIGLEYSLPAGWMLSGNYAYATISSKDVSQQTDFNTPKSKVNL
                     TVANYSIAKVYGFNLTYRWQEGYLYQSSFITGYTPAFGTLDAQISMKLPKLNRSLIKI
                     GASNLLNYYHVDAIGDAMIGGLYYISFGYNVL"
     gene            complement(1847..>2905)
                     /gene="ychF"
                     /locus_tag="VKU83_02875"
     CDS             complement(1847..>2905)
                     /gene="ychF"
                     /locus_tag="VKU83_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLI92520.1"
                     /translation="KSTLFNAVSNSAKAQASNYRFCTIEPNTGLVDVPDERLDKLAAL
                     VKPHRIVPTQLQIVDIAGLVRGASKGEGLGNKFLANIREVDAIVHVIRCFEDENILRD
                     EGDINPVADKEIIDTELQLKDLESVEKKLARAEKMLKTADPKVKAEYAVLRRCYEHLE
                     KGRNIRELGLSKEERGAIADLFLLTEKPVLYVANVDEASMHTGNEFSKALVAAVAGEQ
                     AQVIVMNNSIEAQIAEMDDPADRQLFMEEYRMTEPALNKLIRTAYRLLHLATYFTAGE
                     QEVRAWTIHEGWKAPQAAGVIHTDFEKGFIKAEVISYQDYVQFGSEAAARENGRLRIE
                     GKDYIVKDGDVMHFRFNV"
BASE COUNT          614 a          856 c          803 g          632 t
ORIGIN      
        1 aaagaactac aggcagccgc cgcagggtac caggccgccg caaaccaggc cgccctggca
       61 ggcaacgccg ccctggccgc ccaataccag atgcaggctc agcaggccag ccagctcgcc
      121 gcggcgacgc ccagcaattc caacgtaacc cgcaccggct atgccgagag cgcgcttacg
      181 aattaccggg ccgataactt caaggtttac ggcgggctgt attataaagt gacgccgggt
      241 tcgaccctaa gcctcatcgg caattggggc acggccagca cgatctacca gggaacggac
      301 cggtacagcc tcaaggatgt caggatcggc cagtacaaac tcgagctgac cggccagcac
      361 tattacgtgc gggcatatac cacgcaggaa aatgccggga actcctacga tatggtggcg
      421 gtggccacgc tgatgaacga aaagtatgcg ccgaccgcaa ccgtttgggg tccgacctat
      481 atcggggcct atgcccagtc ctttggggca gacctgcagg cgggccagga tcccgcgaca
      541 gcctatgcca acgccagcgc ggttgcgcgc ggggctgccg atcagaaccg gttgcagcca
      601 ggctcgccgg ctttcaacca ggcgttcaac accttgaagc aacaggcgat cccctatggc
      661 ggcaaattca atgaccagac caacctgtat gtcggggaag cgatgtataa ttttgccaac
      721 gaggtcaaat ggatcgatat taccgtcggc gccgccaccc gcgaattcgt tttgaactca
      781 catgggacca tatttgccga caccgcgggc cgcatcaata tcaatgagag cggcgcattt
      841 gcgcagctgc aaaagggttt cctggacgac ttgctgaaac tttccgtcgc ggggcgctac
      901 gacaagaact cgaatttcaa gggccggttc acgcccaggg ttacggcgtt gctccgggtt
      961 gcgcccgaca actatatccg cgcgtctttt caaacggcct accggttccc gtctacgcag
     1021 aaccagtaca tcaacctgca gaccggttca gcgcggctga tcggcggctt gccgcagttc
     1081 attacggcct atggcttgaa tgacgggcag acctatgata cggcgcaact taacaaatac
     1141 atggcgacgg gaacgccgcc tacggcatat caataccaga ccttcaagcc ggaaacggtg
     1201 gccagctggg aggtcggtta taaggggctg atcgggaaga accttttgtt ggatgtctac
     1261 ggttattacg cgcagtacac gaattttatc gggctgaccg tgctggtgaa ggatccgctg
     1321 acccttggcc agacggccgc aaacactttt ggcatttata cgaacagtcc gaccaaggtg
     1381 aatacgggcg gcgccgggat cgggctggaa tattccctgc ccgcgggctg gatgctctcc
     1441 ggaaattatg cgtacgccac gatctccagc aaggacgtca gccagcagac ggacttcaat
     1501 acaccgaaat cgaaagtcaa tttgaccgtc gccaactatt cgatcgccaa ggtctatggc
     1561 tttaacctga cctatcgctg gcaggaaggc tacctgtacc agtcgtcttt cattacgggt
     1621 tacacgccgg ctttcggaac gctggatgcg cagatcagca tgaagctgcc gaaactcaac
     1681 cgttcactga taaagatcgg ggcttcgaac ctgttaaact actaccatgt ggacgccatc
     1741 ggcgatgcga tgatcggcgg cctgtattat atcagcttcg gctataacgt gctttgatct
     1801 cccccaatcc ccagacccgg agcctgtctg cagacaggct ctttttttat acattaaacc
     1861 ggaagtgcat gacatcccca tccttgacga tataatcctt tccttcgatc cgcagcctgc
     1921 cgttttcgcg tgcggcggct tccgacccaa actggacata gtcctggtaa gaaataactt
     1981 ctgctttgat aaaccctttt tcaaagtccg tatggatcac ccctgcggcc tgcggagctt
     2041 tccagccttc atggatcgtc cacgcgcgga cttcctgttc gccggccgta aagtaggttg
     2101 cgaggtgaag cagccggtag gcggtccgga tcagtttgtt gagggccggc tcggtcatcc
     2161 ggtattcttc catgaacaac tggcggtcgg ccgggtcatc catttcggcg atctgcgctt
     2221 cgatcgaatt gttcattacg atgacctgtg cctgttcgcc ggcaacggcc gcaaccaggg
     2281 ctttcgagaa ttcgttaccc gtgtgcatgg acgcctcatc gacattcgcg acatacagga
     2341 ccggtttttc ggtgagcagg aagaggtcgg ctatggcccc gcgttcttct ttcgacaggc
     2401 ccagctcgcg aatattgcga cccttttcca gatgctcata gcaacggcgg agcaccgcat
     2461 actctgcctt gaccttcggg tccgccgtct tgagcatttt ctccgcccgg gccagtttct
     2521 tttcgacgct ttccaggtct ttcaactgca actcggtgtc gatgatctcc ttgtctgcga
     2581 ccgggttgat gtcgccctcg tcacgcagga tattctcgtc ctcgaagcag cggatgacgt
     2641 gcacgattgc atcgacttcg cggatattcg ccaggaattt gttgccgagc ccttcgcctt
     2701 tgctggcgcc ccttacgagt ccggcgatat cgacgatctg caactgcgtg ggcacgatgc
     2761 ggtgaggctt aaccagcgcc gcaagcttgt ccagccgctc gtcggggaca tcgaccaggc
     2821 cggtattcgg ttcgatggtg cagaaccggt aattgctggc ttgtgctttg gcgctgttgc
     2881 tgacggcgtt gaataacgtc gattt
//
LOCUS       DATGKE010000166         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1102347, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000166 DATGKE010000000
VERSION     DATGKE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102347"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VKU83_02880"
     CDS             complement(<1..565)
                     /locus_tag="VKU83_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92521.1"
                     /translation="MAVISIIFDPTVFPDSLFADHKHPGRAGRVTVTRKMVGDLKDII
                     PYNIFKEMNWQQLYSPVRTGSGTDSRRQDLAESIDAAGGTGSSGRGQEAGELRTSFLR
                     DYDRIIFSSAFRRLQNKTQVFPLPGPVFVHNRLTHSLEVASVGRSLGKAVGDALAEKH
                     SGLGENFREFYKYELASVIAAACLAHDI"
     gene            complement(594..1463)
                     /locus_tag="VKU83_02885"
     CDS             complement(594..1463)
                     /locus_tag="VKU83_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLI92522.1"
                     /translation="MRLYRTKKGNLLADQEKFWLFDKAWDELINRNDLHGYLARAALR
                     EDEVTAMEAADLLEQELLAPIGSQEVWAAGVTYLRSRDARMEESKDSGAASFYDKVYE
                     AERPELFFKSLPHRVAAHGEKVYIRRDSSWNVPEPELTLFINTQGHIQGYTIGNDMSS
                     RSIEGENPLYLPQAKVYDRSAALGPGLYVPAEPIGRDTVIRMSIRRGANLLYRGEVPI
                     SQIKRSLTDLAGWLYRESDFPAGCFLMTGTCLVPANDFTLKEGDNVEISIDGIGTLYN
                     AVGYRPNCRNNIS"
     gene            1578..>2176
                     /locus_tag="VKU83_02890"
     CDS             1578..>2176
                     /locus_tag="VKU83_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92523.1"
                     /translation="MGRNRAETGPRGLSLRNLSIRLQLPLLTCLLLLILIVLFGTVSY
                     IGFRRATMSIGQERLRSLTTELSTLFQQSARTLTAATRGIATGDTIVQFVSRGLRQSP
                     AGNRAPAIALQKILDADSVTVRMELRDTAGNIMLAVDQRRRAKKAIPPWNVESPMGGA
                     ATATDGIGKNPDSSFVDKFYVIGDSVFYPVVASIKANQRT"
BASE COUNT          448 a          663 c          591 g          474 t
ORIGIN      
        1 cgatatcgtg cgccaggcac gctgccgcga tcacggatgc cagctcatat ttgtaaaatt
       61 cccgaaaatt ctcgcccagc ccggaatgct tctccgccag ggcatcgccg accgccttgc
      121 ccagggatct gccaacggac gcaacctcga ggctatgcgt caggcggttg tggacgaaga
      181 ccggtccggg gagggggaag acctgcgttt tgttttgtag ccggcggaag gccgaggaaa
      241 aaatgatccg gtcatagtcg cgcagaaaag aagtgcggag ctctccggct tcctggccgc
      301 ggccggagct gcccgtgccg ccggccgcat ctatgctttc cgcgagatcc tgccgccgtg
      361 aatcggtccc gcttcctgtc cttaccgggc tatataactg ctgccaattc atctctttaa
      421 agatattgta gggaatgata tctttcaggt caccgaccat cttccttgtc acggtcaccc
      481 ggcctgcgcg gcctgggtgc ttgtgatcgg caaaaagact gtcggggaat accgtcggat
      541 caaaaataat tgaaataact gccaccctcg ctggtgacga tcgcgcggca actttaactt
      601 atgttgttgc ggcagttggg cctgtatccg acggcgttgt acaaggttcc gatgccgtcg
      661 atcgagatct cgacattatc cccctccttt agcgtgaaat cattggcggg aaccaggcag
      721 gtgccggtca tcaggaagca gcccgccgga aaatcggatt cccggtaaag ccagcctgcc
      781 aggtccgtga gggaccgctt gatctggctg atgggaactt cgccgcgata aagaagattg
      841 gcgccgcgac ggatcgacat ccggataacg gtgtcgcggc cgatcggctc cgccggcaca
      901 tagaggcccg gacccagcgc cgcgctccgg tcgtagacct ttgcctgggg aaggtacaat
      961 gggttctccc cttcgatact gcgggaactc atgtcattgc ctatcgtgta tccctggata
     1021 tgtccctggg tattgatgaa cagggtcagt tcgggctcgg gcacattcca cgaggaatcg
     1081 cgccggatat agactttttc accatgcgcc gccacgcgat ggggcagtga tttgaaaaac
     1141 agttccggcc gctctgcttc gtacactttg tcatagaagc tggcggcccc ggaatccttc
     1201 gattcctcca tcctcgcgtc gcggctgcgc agataagtga cccccgctgc ccagacttcc
     1261 tgcgacccga tcggcgccag caattcctgc tcgagcaggt ccgctgcttc catcgccgtg
     1321 acctcgtctt ctctcaacgc agcccgcgcc aggtagccgt gcaggtcgtt gcggttgatc
     1381 agctcatccc acgctttgtc gaagagccag aatttctcct gatccgccaa taaattgccc
     1441 tttttcgtcc tgtaaagccg catcttgcct tttttcttca ggcaagctag gcagattaaa
     1501 ccaaatttaa ttcccggcca ataaaaaatt ttgtatttta tccccgcctc aaacaccaaa
     1561 agaaaaacgc tgcctgtatg ggacgtaaca gggccgaaac cggaccacgt ggcctgtcgc
     1621 tccgcaattt atctatccgg ctgcagttac cgctgctgac ctgtttgtta ttgctcatcc
     1681 tcatcgtgtt attcggaacg gtatcgtaca tcggcttccg ccgcgctacc atgagcattg
     1741 gccaggagcg gctgcggagc ctgacaaccg agctgtccac ccttttccag cagtcggcgc
     1801 gaaccttaac ggctgccacc cgggggatcg ccaccggaga caccatcgtt caatttgttt
     1861 cccggggctt gcggcagtcg cccgctggca accgggcgcc cgccattgca ctccaaaaga
     1921 tcctcgacgc cgactcggtc accgttcgga tggaactgcg ggataccgcc ggaaacatca
     1981 tgctggccgt ggatcagcgc cgccgcgcga aaaaagccat tccaccctgg aatgtcgaaa
     2041 gtccaatggg gggcgccgcc accgccacgg atggcatcgg caaaaatccc gactcgagct
     2101 tcgtagacaa gttctatgtt atcggcgatt cggtgtttta ccccgtggta gcaagcataa
     2161 aagccaacca gcgaac
//
LOCUS       DATGKE010000167         6772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_685296, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000167 DATGKE010000000
VERSION     DATGKE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685296"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VKU83_02895"
     CDS             <1..1128
                     /locus_tag="VKU83_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLI92524.1"
                     /translation="NELNGARSRIRYFLDHSAKWLAEEWMVTDGATREKIVYEPLGIV
                     ANISAWNYPYLVGVNVFVPALIGGNAVLYKPSEYATLTGLHIRRLLFRAGVPENVFQL
                     VTGRGRAGEMLLELPLDGYFFTGSYRTGKSIAEKVAPKLVPCQLELGGKDPLYVMDDV
                     ADIDQVAGAALEGVVYNNGQSCCAVERIYVHEKVYEAFVRSYTEQVRKMVVGDPRVPG
                     TDIGPLSRAGQLDFLLQQIEDALSKGARLLCGGRRVDGKGYFIEPAVLVNVDHRMRLM
                     TEESFGPVVGIQRVKDDEEAVERMADTEYGLTAAVYSADYRRAERVMQRLNTGTVYWN
                     CCDRVSATVPWSGRKHSGLGATLSYQGIRAFVQPKAYHLRG"
     gene            complement(1426..2133)
                     /locus_tag="VKU83_02900"
     CDS             complement(1426..2133)
                     /locus_tag="VKU83_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLI92525.1"
                     /translation="MNRYPGETRLLVAVDCIIFGFDGSDIKLLLIQRGLQPKKGHWSL
                     MGGFLKPKESLDEAANRVLKKLTGLEGVYMEQMAAFGNPDRDPVERTVSIAYFALIDI
                     HQYARQLTTEYHAEWFHLKKMPDLIFDHRRMVDIAKRQLRYKAALHPILFELLPPRFT
                     IPQLQILYESIYDTNFDNRNFSRKVLSTELLVKQKEKDKTGSKKGAFFYRLDKRKYKA
                     NFHAFLNFIPNPDKLLN"
     gene            complement(2172..3884)
                     /locus_tag="VKU83_02905"
     CDS             complement(2172..3884)
                     /locus_tag="VKU83_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HLI92526.1"
                     /translation="MNSNLDFSDKIVFLIYFLIVAFYGTWIYRRKKKAVTDTKDFFLA
                     EGTLTWWAIGASLIASNISAEQFVGMSGDGFFAGIAVAAYEWIAAVALIIIAVWFMPV
                     YLKNKIFTMPQFLKMRYNETVSLIMAIFWLLLYIFVNLTSIIYLGAVAINGLLGGQYL
                     HIVMILLGVFALIIALGGMKVIGYTDVIQVCVLIIGGLATSYLALTVVAKTFGLGDNA
                     FAGFTAMMHDAPEHFRMIFPKPTASTSQLEINKYLILPGLAMYVAGQWIINLNYWGCN
                     QYITQRALGANLKTARTGILFAGFMKLLMPVIVMLPGIAAFVLYKHGNLHLEGGNKDY
                     AYSAVLGFLPAGLKGLAIAALTAAIVSSLAGKINSISTIYTLDIHKKYFDKTADERKM
                     VWTGRFVIVVAMIIGIIFTWTDVLGISGTGGFTYIQKYTGFISPGVLAMFLFGMFWKR
                     TTGAAAVGGLITGFILSVFFNEPRFAPEVFGHNTWLYTAYPTQVLEHGQMVTVYEIPF
                     AVAMGLAFLFTALIMIAISLGGPKVNAKAFIFEKGMFKVEPRTVALIVVTLLILAAIY
                     VKFW"
     gene            complement(3916..5412)
                     /gene="araA"
                     /locus_tag="VKU83_02910"
     CDS             complement(3916..5412)
                     /gene="araA"
                     /locus_tag="VKU83_02910"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF002795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HLI92527.1"
                     /translation="MDLKSLEAWFVTGSQHLYGEATLQQVAEHARIIAKGLNDFAGIP
                     VHVVYKPTVKSPEEIFRICQEANNARSCIGIIAWMHTFSPAKMWIGGLKILQKPIAHL
                     HTQFNRDIPWAQIDMDFMNLNQSAHGDREFGFMMARMRLNRKVVTGHWQDPAVLQRLN
                     AWLRAAAGWHDWQGARFVRFGDNMRQVAVTEGDKVEAEMTFGYSVNTHGIGDLVQVIG
                     DTPDADTDRLVKEYEERYIVAAALRKGGPRHQSLKDAARIELGLRRFLEEGNFKGFTD
                     TFEDLHGMTQLPGIAAQRLMGSGYGFAGEGDWKTAALVRAMKVMGSGLPGGNSFMEDY
                     TYHFEPGNNLVLGAHMLEICESIADGKPSCEIHPLGIGGKADPVRLVFNSAAGPAINA
                     SIIDMGNRFRLIVNEVEAIAPAHSLPNLPVARVLWRPYPDMATGCSAWIMAGGAHHTG
                     YSQNLTAEHMRDFADIIGIELALIGKNTDIYQFRNELRWNEAWFGIKR"
     gene            complement(5423..6121)
                     /locus_tag="VKU83_02915"
     CDS             complement(5423..6121)
                     /locus_tag="VKU83_02915"
                     /inference="COORDINATES: protein motif:HMM:NF006047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HLI92528.1"
                     /translation="MSKYNHIQEAAFEANRQLPKLGLVLFTFGNVSAADRGLGVFAIK
                     PSGVPYEDLSPEKMVIVDFDGKTVKGKLRPSSDTFTHALLYKEWPEVGGIAHTHSTYA
                     TAWAQSQRDIPIFGTTHADHATVDIPCAPPMDDEMIQGDYEYQTGFQIIHCFRNRRLN
                     YKEVEMILVGNHAPFTWGPTPQKAVYNSAVLESIAQLAYLTLQIDVSAPRLKESLIKK
                     HYERKHGPNAYYGQ"
     gene            complement(6132..>6772)
                     /locus_tag="VKU83_02920"
     CDS             complement(6132..>6772)
                     /locus_tag="VKU83_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLI92529.1"
                     /translation="QSAFGDAYAWFRDLLAWPLHQFAPDRPELVDKVLPELSRRAALL
                     TPSEQAPLALDWLNGRRSPDANQLLKAAVTGLNLGTDAPQLFRSIVEATCFGARAIVD
                     RFQEEGVPIRGLIGLGGVARKSPFVMQMMADVMDRPLRIHASEQTCAFGAAMFAATAA
                     RLYPNVETAMAAMGQGFDASYSPDAGRAAYYARRYQQYRTLGQFAESTTILL"
BASE COUNT         1444 a         1695 c         2099 g         1534 t
ORIGIN      
        1 aacgagctga acggggcgcg atcccggatc cggtatttcc ttgaccattc tgcaaaatgg
       61 ctggccgaag agtggatggt aacggacggg gctacccgcg aaaagatcgt gtacgaaccg
      121 ctgggcatcg ttgccaatat ctcggcctgg aattaccctt accttgtcgg agtgaatgtt
      181 tttgtgccgg cgttgatcgg cggcaacgcc gtgctgtaca aaccatcgga gtatgccacc
      241 ctaacagggt tgcacatccg ccggctgctt ttccgggccg gcgttccgga gaatgtcttc
      301 cagctggtga cgggcagggg cagggcaggg gaaatgctgc tggagcttcc gctggacggg
      361 tattttttca cgggcagcta ccggacgggc aaatcgatcg cggaaaaagt ggcgccgaag
      421 ctggtacctt gtcagctcga gcttggcggt aaagaccctt tatatgtcat ggatgacgtc
      481 gccgatatcg accaggtggc cggggcggcg ttggaaggag tagtgtacaa taatggtcaa
      541 agctgctgcg ccgtcgagcg gatctatgtg cacgaaaagg tctatgaggc gtttgtccgg
      601 tcctatacgg aacaggtacg gaagatggtc gttggtgatc cgagggtccc gggtaccgat
      661 atagggccgt tgagccgggc aggtcagctc gactttctgc tgcagcagat cgaagacgca
      721 ttgagcaagg gcgccaggtt attatgtggg ggaaggcggg tcgacggaaa gggctatttt
      781 atcgagccgg cggtcctcgt gaatgtcgat caccggatga gactgatgac cgaggaatct
      841 ttcggcccgg tggtgggcat acagcgcgtg aaggacgatg aggaagcggt ggaacggatg
      901 gctgacacgg aatacggctt gacggcagcg gtttattcag ctgattacag gcgggcggaa
      961 cgggtcatgc agcgcttgaa tacgggaact gtgtattgga actgctgtga ccgggtgagc
     1021 gcgacggttc cctggtcggg aaggaagcat tccgggcttg gcgcgacctt gtcttaccag
     1081 ggtatcaggg catttgttca accgaaagcg tatcacttaa ggggttagcg gcacagcttg
     1141 cccgttcgcc gaagcttcgg ctggcgaatc cggtctgggc agggctttca agataagagg
     1201 gcaatagaaa ttgccctcgc gtcacaactt tgtgaccatt caaaactaac atttataagc
     1261 aggtaatttt ttggcagagc aatccaaaga caggtagcca gcaaatgtac gactccgcgc
     1321 cgacgtcgca ttatccctgg cactaaaaat caccgtagtg gggcaaaatt atccgcaaac
     1381 gtttgggccc tggcgcttgg gggtcttgtt cggctgcatc tgccatcaat tcaataattt
     1441 atccgggtta gggataaagt tgaggaacgc gtgaaaattc gccttgtatt ttcgtttgtc
     1501 gagccggtag aagaaggccc cttttttgga gcccgtctta tctttctctt tttgcttgac
     1561 cagcaattcg gtggaaagga ctttgcggct aaaattccgg ttgtcgaaat tcgtgtcata
     1621 aatgctttca tataggatct ggagctgagg gatagtgaag cgcggtggca gcagctcgaa
     1681 aaggatgggg tggagcgcag ctttatagcg aagctgccgc ttggcgatat ccaccatccg
     1741 gcggtggtca aaaatgaggt cgggcatttt tttcaggtgg aaccattcgg catggtattc
     1801 cgtggtcagc tggcgggcgt attgatggat gtcaatcagc gcgaagtatg cgatgctgac
     1861 ggtgcgttca accgggtcac ggtcagggtt gccgaaggcc gccatttgtt ccatatacac
     1921 gccttccagc ccggtgagtt ttttcagcac gcggttggcg gcctcgtcga ggctttcttt
     1981 gggtttgagg aagcctccca tcaggctcca gtggcccttt ttcggttgca ggccgcgttg
     2041 gatcagcagc agtttgatat cgctgccgtc aaatccaaag atgatgcagt ctacggcaac
     2101 cagcaggcgg gtttcacccg ggtaacgatt cattcgaatg tttttttgtc aaatatgcgc
     2161 gtgaattttt tttaccagaa cttgacatag atcgcagcaa ggatcaacag cgttacgaca
     2221 atgagcgcaa cggttctcgg ttcgaccttg aacatccctt tctcaaagat gaatgctttg
     2281 gcgttgacct ttggaccgcc gaggctgatg gcgatcatta tcagggccgt gaacaggaag
     2341 gccaggccca tggcgaccgc gaagggtatc tcatagacgg tgaccatctg gccgtgctcc
     2401 aatacctggg tcgggtaagc ggtatacaac caggtattgt gaccaaatac ttccggggca
     2461 aacctgggtt cgttgaaaaa cacgctgagg atgaatccgg tgatcagacc gccgaccgcg
     2521 gctgcgccgg tcgtccgttt ccaaaacatt ccgaacagga acatggccag gacacccgga
     2581 ctgatgaaac ccgtgtattt ctggatatag gtgaagcctc cggtgccgct gatcccgagc
     2641 acatccgtcc aggtaaaaat gatgccgatg atcatggcta cgacgataac gaaacgtcca
     2701 gtccagacca ttttcctttc atccgcggtc ttatcaaaat attttttatg gatatcgagg
     2761 gtatagatcg tggaaatgct gttgatcttg cccgcaaggg aggaaacaat ggcggcggtc
     2821 agggctgcga tggcgagacc ttttaagccg gcgggcagga aacccaggac ggcagagtag
     2881 gcataatctt tatttccgcc ttcaagatgg aggtttccgt gtttgtagag tacaaatgcg
     2941 gcaataccgg gcagcatgac gatcacgggc atcaggagct tcataaagcc ggcgaaaagg
     3001 atgccggtgc gcgccgtctt gagattcgcg ccgagggcgc gctgggtgat gtattggttg
     3061 cagccccagt agttcaggtt aatgatccat tggccggcga cgtacatggc gagacccggc
     3121 aagattaagt atttgttgat ctccagttgg ctggttgagg cggtgggttt gggaaagatc
     3181 atcctgaaat gttccggagc gtcatgcatc atggcggtga aacctgcgaa ggcattgtcg
     3241 ccgagaccga aagtcttcgc gacaacggtg agcgcgagat aagaggttgc caggccgccg
     3301 atgatcagga cgcagacctg gatgacatcg gtataaccga tcaccttcat tccgcccagg
     3361 gcaataatca gggcgaagac acccaggagg atcattacga tgtggaggta ttggccgccc
     3421 aggaggccgt taatggcaac ggcgccaagg tagattatgg aggtaaggtt gacgaaaatg
     3481 tagagaagca gccagaagat ggccatgatc agggataccg tctcgttata gcgcatcttg
     3541 aggaactgtg gcatcgtgaa gatcttgttc ttaagataga ccggcatgaa ccagacggcg
     3601 atgataatga gggcaacggc tgcgatccat tcataggcgg cgaccgcgat gcccgcgaag
     3661 aatccgtcgc cgctcattcc gacgaattgt tcggcggata tattggaagc gatcaatgag
     3721 gcgccgatgg cccaccaggt gagcgtacct tcggcgagga agaagtcctt tgtatccgtg
     3781 acggctttct ttttccgccg gtagatccag gtaccataaa acgcaacgat caggaaatag
     3841 atgaggaaga cgatcttgtc ggagaagtcg aggttactat tcatgagcaa tcgttttttg
     3901 tgcggatggt ccgggttaac gtttgatacc gaaccaggct tcattccacc gaagctcgtt
     3961 ccggaattgg taaatatctg tatttttccc gattagcgcc aattcgatgc cgataatgtc
     4021 ggcgaagtcg cgcatgtgtt cggcagtgag gttctggcta tagccggtat ggtgcgcgcc
     4081 cccggccatt atccaggcgg agcagccggt cgccatatcc gggtagggcc tccagaggac
     4141 gcgggccacg ggcagattcg gcagtgaatg ggccggggcg atggcttcca cttcgtttac
     4201 gatcagccga aagcggttac ccatgtcgat tatggaagcg ttgatcgcgg gtccggcggc
     4261 cgaattgaaa accagtcgta ccgggtccgc ctttccgccg atgccgaggg ggtggatctc
     4321 gcaggaaggt ttgccgtcgg caatggattc gcagatctcg agcatatgcg cgccgaggac
     4381 gaggttattg cccggttcga aatgataggt ataatcttcc atgaacgaat tgccgccggg
     4441 caggccgctg cccatcacct tcatggcgcg gacaagggcg gccgtcttcc agtctccttc
     4501 cccggcgaat ccgtaaccgc tacccatgag ccgctgggcg gcgatgcctg gtaactgcgt
     4561 catgccgtga aggtcctcga acgtatcggt gaagcctttg aaattaccct cttcgagaaa
     4621 acggcgaagg ccaagctcga tgcgggccgc gtccttcagc gactgatgtc gcgggccgcc
     4681 tttccggagc gctgccgcga cgatataccg ttcttcatat tccttgacga ggcggtcggt
     4741 gtcggcatct ggcgtatccc cgatgacttg caccaggtcg ccgatgccat gcgtattgac
     4801 ggaatagccg aatgtcattt cggcttcgac cttgtcgcct tccgtgaccg ccacctggcg
     4861 catattgtca ccgaagcgaa caaatctggc gccctgccaa tcgtgccagc cggcggcggc
     4921 gcgcaaccac gcgttgaggc gttgcaggac ggcggggtcc tgccaatggc cggtcactac
     4981 ctttctgttc aggcgcatcc gcgccatcat gaacccgaat tcgcggtcgc catgagcgct
     5041 ctggttcagg ttcatgaaat ccatgtcaat ttgtgcccag ggaatgtcgc ggttgaactg
     5101 cgtgtgcaga tgagcgatgg gtttttgcaa tattttgagc ccgccgatcc acattttggc
     5161 gggtgaaaag gtatgcatcc acgcgataat gccgatgcag ctgcgggcat tgttggcttc
     5221 ctggcaaatc cggaagatct cttccgggga ttttacggtc ggtttgtata cgacatgcac
     5281 cggaatcccg gcgaagtcgt tgaggccttt tgcaatgatc cgcgcgtgtt ccgctacttg
     5341 ttgcagggtt gcttctccgt agaggtgctg gcttccggtg acgaaccagg cctcgagact
     5401 ttttagatcc atatttggtt ggttattggc cgtagtaggc gttggggccg tgtttcctct
     5461 cgtagtgttt tttgatcaga gactctttca acctgggcgc gctgacgtcg atttgcaggg
     5521 taaggtaggc caactgggca atggactcga ggacggcgct gttatagacc gctttttgag
     5581 gcgtcggccc ccaggtaaaa ggcgcatggt tgcccaccag gatcatttcc acctctttgt
     5641 agttcaggcg gcggttgcga aagcaatgga tgatctggaa gccggtctgg tattcgtaat
     5701 ccccctggat catctcgtcg tccatagggg gtgcgcaagg gatgtccacg gtggcatggt
     5761 cggcatgggt ggtgccaaag atggggatat cgcgttggga ctgcgcccac gccgtggcgt
     5821 atgtcgagtg cgtgtgggcg atgccgccga cctccggcca ttccttgtaa agcagggcat
     5881 gcgtaaaggt gtcggaagac ggacggagct tacctttaac ggtctttccg tcgaaatcca
     5941 cgatgaccat tttctccggt gacaaatctt cgtacgggac gccgctcggt ttgatcgcga
     6001 acacgccgag gccgcggtcg gcagcgctta cattgccgaa ggtgaacagg acaagcccaa
     6061 gtttcggcag ttgccggttg gcctcaaagg cagcctcctg gatatgattg tatttactca
     6121 tgatgtggcc ctcataatag tatcgttgtg gattctgcaa attgtccaag cgtcctgtat
     6181 tgttggtagc ggcgggcata atatgccgcg cggcccgcat ccggggaata ggatgcgtcg
     6241 aatccctgtc ccatggcagc cattgcggtt tccacatttg ggtaaaggcg cgctgccgtg
     6301 gcggcgaaca tggcagcccc gaaggcgcag gtttgctccg aagcgtgaat gcgaagcggg
     6361 cggtccatga cgtccgccat catttgcatg acaaaggggg atttcctggc tacgccgccg
     6421 aggccgatca gccccctgat gggaacgcct tcttcctgaa agcggtccac gatcgcacgc
     6481 gcgccgaagc aggtagcttc cacgatggaa cgaaagagct gcggcgcatc ggtaccaagg
     6541 ttgagaccgg tgaccgccgc tttaagaagc tggttggcgt cgggactgcg gcggccgttg
     6601 agccagtcga gggcaagcgg cgcctgctct gagggggtca gcagggcggc gcggcggctc
     6661 agttcgggaa ggactttgtc aacgagctcc ggccggtccg gcgcgaattg atggagcggc
     6721 caggcaagga gatcgcgaaa ccaggcgtat gcgtcgccaa acgcggactg cc
//
LOCUS       DATGKE010000168         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1201866, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000168 DATGKE010000000
VERSION     DATGKE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201866"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /gene="ligD"
                     /locus_tag="VKU83_02925"
     CDS             <1..2429
                     /gene="ligD"
                     /locus_tag="VKU83_02925"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HLI92530.1"
                     /translation="EALEAPGGTKAAQEKALLRQLHEGSLKFRLNGKKLKGEFALVRT
                     KGRGENSWLLIKHRDRYATEDPVTDKDKSVVSKKTLEQIAGNPRAAQWSSNRPAAGTG
                     VKGGSGKRSAGKPAPARESGAKRTASAAVHPAIPAAAPRRPMPKDVPPMLATLVDKPF
                     DGEGWSYEVKWDGYRAIAYVNQGKVELRSRNNKTFEKYYPIYEALREWPVNAVVDGEI
                     VVLDENGQSDFGKLQNWRSEADGFLVYYVFDLLWLDGRDLTGLPLTDRRDLLRTIVPG
                     NDPIRYSESFVTSGKTFFEKARQLKLEGIMAKKMDSLYTPGSRTREWLKIKTASRQEV
                     VIGGYTRNEDSPKLFSALLVGVFEDGKLQYTGKVGTGFTEQLQRDMMKQFKPLVQKKS
                     PFNVEPDINQPSRFRPDPPTAKATWLKPVLVGEVAFREMTSDGVMRHPSFQGMREDKE
                     AKDVVRETPKPAEAILGEAAAPGPRRRPSRRKTAVADRILSPVGVKDRKTLLNPADET
                     QTREINGHELSFTHLSKIFWPKEKYTKRDLLNYYYQLAPFILPYLKDRPQSLNRHPNG
                     ITGKGFYQKNVSGKVPDWIKTFPYHSAADAEDKEFLVCTDEASLLYMVSLGCIELNPW
                     SSRVNSPDNPDWCIIDLDPAERTPFEKVIEAAQVTKKVLDSAGIDSYCKTSGSTGLHV
                     YFPLGAKYSYEHSKEFARLVVQRVHEEIPAFTTVERMVAARRGRMYLDFLQNRPQATI
                     AAPYSVRPKPGATVSAPLRWEEVKKGLSMQDFTIRNMAARLKEAGDLFQPVLGKGISM
                     EAALKKLNSL"
BASE COUNT          630 a          746 c          766 g          428 t
ORIGIN      
        1 atgaagccct cgaagcgccg ggaggcacca aggcggcgca ggaaaaagcg ctgttacgcc
       61 aactgcatga aggatcgttg aagttccggc taaacggtaa aaagctcaag ggtgaatttg
      121 ccctggtccg gacgaagggc cgcggagaaa attcctggct gctgatcaaa caccgcgacc
      181 gctatgctac agaggacccg gtgacggata aagacaaatc ggtggtctcg aagaagaccc
      241 tcgaacaaat cgccggcaac ccgcgtgccg cccaatggag cagcaaccgg cctgccgcgg
      301 gaaccggggt gaaaggcggc agcgggaaac gaagcgcggg gaagccggcc cctgccaggg
      361 agagcggggc gaagcgcact gcaagcgcgg cggttcatcc ggccatccct gccgccgctc
      421 ccaggaggcc catgccgaag gacgttccgc cgatgctcgc gacgctcgtc gacaaacctt
      481 tcgacgggga aggctggtct tacgaagtaa aatgggacgg ctaccgggcc atcgcctacg
      541 tgaaccaggg gaaggtcgaa ctccgttccc ggaacaataa aaccttcgaa aagtattatc
      601 cgatctatga agccctgcgg gagtggcccg tcaacgccgt ggtggatggc gagatcgtcg
      661 tccttgatga aaacggccag tccgatttcg ggaaactgca gaactggaga agcgaagccg
      721 acggtttcct cgtgtactac gtttttgatc tgttgtggct cgacggacgc gatttgaccg
      781 gtctgcccct gacggatcgg cgcgacctgc tccggacgat cgttcccggg aacgacccga
      841 tccggtacag cgaaagtttc gtgacgtcgg gaaaaacctt tttcgagaaa gccaggcaat
      901 tgaagctcga aggcatcatg gcaaagaaga tggacagcct ttataccccg ggcagccgca
      961 cccgggaatg gctgaagata aagaccgcca gccgccagga agtggtgatt ggcggctaca
     1021 cccggaatga agactcgccg aaactgttca gtgccctgct ggtcggcgta ttcgaggacg
     1081 gcaagcttca atacacgggg aaggtcggta caggatttac cgagcagctt caacgcgaca
     1141 tgatgaaaca attcaagccg ctggtccaaa aaaaatctcc tttcaacgtt gaacccgata
     1201 tcaaccagcc ctcacggttc cggccggatc cccccaccgc caaggctacc tggctgaagc
     1261 ccgtgctcgt gggagaagtg gccttccgcg aaatgacctc cgacggcgtg atgcgacatc
     1321 cctctttcca ggggatgcgc gaagacaagg aggcaaaaga cgtcgtgcgc gaaacgccga
     1381 agccggccga agccattctc ggcgaagcgg cggcgcccgg gccgcgccgg agaccatcca
     1441 ggcgaaagac tgccgtggcg gacaggatct tgtcgcctgt cggcgtgaaa gaccggaaaa
     1501 cgctgctcaa tcccgccgac gaaacccaaa cgagggaaat caatggccac gagctgtcct
     1561 ttacgcatct gagcaaaata ttctggccaa aagaaaaata taccaagcgc gacctgctga
     1621 actactacta tcagctagcc ccttttatcc tgccttacct gaaggaccgg ccgcaatccc
     1681 tgaaccgtca cccgaacggc attaccggga aagggttcta tcagaagaac gtgagcggca
     1741 aagtgccgga ctggataaag accttcccct atcacagtgc ggctgacgcc gaagacaagg
     1801 aattcctggt ttgcacggac gaagccagcc tgctatatat ggtttcgctg ggttgcatcg
     1861 aactcaaccc ttggagcagc agggtcaaca gccccgataa ccccgactgg tgtatcatcg
     1921 accttgatcc ggccgaacga acgccgtttg aaaaggtgat cgaggccgcg caggtcacca
     1981 aaaaagtgct ggattccgca ggcatcgact cgtattgcaa gacatccggc tcaacggggc
     2041 ttcatgtcta tttcccgctc ggcgcgaaat attcctatga acactcgaag gaattcgccc
     2101 ggctcgtcgt gcagcgcgtc cacgaggaga tccccgcgtt cacgacggtg gaacggatgg
     2161 tggctgcgag gcgtggcagg atgtacctgg actttcttca gaaccggcca caggcgacga
     2221 tcgctgcgcc ctactcggtg agacccaagc ccggcgcaac ggtttcggct ccccttcgat
     2281 gggaggaggt gaaaaaaggc ctgtccatgc aggatttcac gatccggaat atggcggccc
     2341 gcctgaagga ggcaggcgac cttttccagc ccgtgctggg aaaaggcatc agcatggagg
     2401 cggcgctgaa aaaactgaac agcctgtaac gcggctaaat gacggcgtca ataaaacgct
     2461 gggaccggta caggaaggga atgcttgccg tgctcaggcc aaggatcacc gtaagcccgc
     2521 cgagcaggcc cctgaaaccg ctgtccaggc ccggatcgcc aaagcgcgtc
//
LOCUS       DATGKE010000169         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_376199, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000169 DATGKE010000000
VERSION     DATGKE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376199"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1066)
                     /locus_tag="VKU83_02930"
     CDS             complement(215..1066)
                     /locus_tag="VKU83_02930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLI92531.1"
                     /translation="MRSHTLIVTAIALAAVCLNACHNNSGKEAATAPAGQVPIPAVLK
                     EQTVSVPADGDTLICYLAYNDSIKGKRPAILVLPEWWGLNEYPRMRARMLAKLGYVAM
                     AVDLYGNDKVADSPATAGAYAMPFYKHPEKAKLRIDSAIARLKTFDVVDPHEIGAIGY
                     CFGGGVLLNTVRLGDSLKGVVSFHGDLLGVRPTRNLLRTKILVCHGNADQFVTAKEVA
                     VFKKQMDSIGAPYTFIGYDSATHAFTNPASTANGIKFKMPIAYNPRADTASWKAMTDF
                     FAGLFGK"
     gene            complement(1136..>2087)
                     /locus_tag="VKU83_02935"
     CDS             complement(1136..>2087)
                     /locus_tag="VKU83_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLI92532.1"
                     /translation="LVPDSSYQDIKRRLAGYHSQWYAPPSREGTVIFPGFDGGAEWGG
                     PAYDPETGLLYVNANEMPWILTIVNAAAPPPVSGSTTIGKAARALYLTNCMSCHGTGR
                     QGSGNYPSLIGVSKKYNREQFLALVSNGRRMMPAFKQLSPAEKDALAAYILDEGSRQK
                     LPFVDKTVKPRDPYFRLPYSITGYNKFLTKEGYPAIMPPWGTLNAIDLDKPSIAWRDT
                     LGDYPELKARGIHSGTENYGGPVVTAGGLLFIAATSDAKIRAFNKRTGELLWEASLPA
                     AGFATPAVYSEEGKEYLVIACGGGKLHKRSGDAYVAFALP"
BASE COUNT          437 a          592 c          595 g          463 t
ORIGIN      
        1 caccccgaga tcctgcaggt agtccagctt ctgcagcaag ccctggaaat caccgatccc
       61 gtccgcgtcg ccgtcacaaa acgccttaat gtgcaattcg taaatgatgg catctttgta
      121 ccagtggagg ttgtcgtcga acgcaaacgc cgttgccata ccttctctct tacaaatagc
      181 atgcaaaccg ttatggggaa ttcgctctac actcctactt accgaacaag cctgcaaaaa
      241 aatccgtcat cgctttccag gaagccgtgt ccgccctcgg gttgtaggcg atcggcatct
      301 tgaactttat cccattcgcc gtcgaggccg gattggtgaa agcgtgggta gcgctgtcat
      361 aaccaataaa tgtataggga gcaccaatgg aatccatctg ctttttgaac acggccacct
      421 ccttagcggt aacaaactgg tcggcgtttc cgtgacaaac gaggatcttc gtccgaagca
      481 ggttcctggt cgggcgtaca ccgagtaagt caccatgaaa actcacgacg cctttgagcg
      541 agtcgccaag ccggacggtg ttcagcagga cgccgccgcc gaaacaatag ccgatcgcgc
      601 cgatctcatg tgggtctacc acgtcgaatg tttttagtct ggcaatggcc gaatcaatgc
      661 gcagtttggc cttttcagga tgcttgtaga aaggcatcgc ataggccccg gcggtggccg
      721 ggctatcggc cactttgtcg ttgccataca aatcgaccgc catagccacg tatcccagct
      781 tcgccagcat ccgcgcgcgc attcgcggat actcattcaa accccaccat tccggaagga
      841 ccaggatagc gggccttttt cccttgatgg aatcattgta ggccaggtaa caaatcagcg
      901 tgtccccgtc ggcagggacg gaaacagttt gctcctttaa tacagccggg atcggaacct
      961 ggccagccgg agcggtagcg gcttctttcc cggagttatt atgacaggcg tttaaacaga
     1021 cagccgccag cgcgatggcg gtcacgatca gagtatgact acgcattttt tttgaatttt
     1081 ggtttacaaa attaaggaac ggcgcgaacc gtttcgcggt cagcgggcag gatggtcacg
     1141 gcaaggcaaa agccacatag gcatccccgg atcttttatg cagcttgccg ccgccgcagg
     1201 cgatgacgag gtattctttg ccttcttccg aatacaccgc aggtgtggca aatcctgccg
     1261 cgggaagact ggcctcccat aagagctcac cggtgcgctt gttgaaggcc cggatcttcg
     1321 cgtcgctggt tgccgcgata aacagcaggc cgcccgccgt aaccaccggt ccgccgtagt
     1381 tctcggttcc gctgtgtatc ccccgcgctt tcagctccgg ataatcaccg agggtatcgc
     1441 gccaggcgat ggacggttta tccagatcga ttgcgttaag cgttccccag ggcggcatta
     1501 tggccgggta gccttccttt gtcagaaatt tattatagcc ggtgatcgaa tacggcaggc
     1561 ggaagtaagg gtcacggggt ttgaccgtct tgtcgacgaa gggcagcttc tgcctcgagc
     1621 cctcatcgag aatatatgcg gccaatgcgt ccttttcagc cgggctcagc tgtttgaacg
     1681 cgggcatcat gcggcggcca ttggagacga gggcgagaaa ctgttccctg ttgtattttt
     1741 tgctgacgcc gatcagcgac gggtaattgc cgctgccttg gcggcctgtg ccatggcaag
     1801 acatacagtt ggtcaagtac agggcgcggg cggccttccc gatcgtggtg gacccggaca
     1861 ccggtggtgg cgccgccgcg ttcacgatgg tcaggatcca gggcatttcg ttcgcgttga
     1921 catagagcag tcccgtttcg gggtcataag ccgggccgcc ccattcggcc ccgccgtcga
     1981 aaccgggaaa gatgacggtt ccctcgcggc tgggcggcgc gtaccattgc gagtggtatc
     2041 cggccagccg tcgtttgata tcctggtagg aggaatcggg aacgagg
//
LOCUS       DATGKE010000170         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_789601, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000170 DATGKE010000000
VERSION     DATGKE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789601"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="VKU83_02940"
     CDS             <1..2140
                     /locus_tag="VKU83_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92533.1"
                     /translation="KHFDNHVGDQYQSAAIITVPDYFRVFPYRWLAGNAAVLDRQDEL
                     VLTESRGRLYFGNIPPAEMVGKMVVYDDSLQVHVGGVVRDLAGNTDMGYTDFISIATA
                     THSFLKDWIPTADWKSLRPHQSMAWVKLAKGVTAGQVNASFAEYIRQHPPRGRGTLNF
                     YLQPLEDIHFTRNFHRGEGDMGDDGDMWRKPYLPELDAMIGVAIFILLIAAINFINLS
                     TAQSMSRAKEVGVRKVMGSRVSHLRFQFLVETMLVVLLAVILAVSLVNPVLHLFQAYL
                     PRGVRFELAYSGTLAFLAGLILVTTLLAGAYPAWVMARYRPVESLKGEISGAGPGSLH
                     LRRALIVFQFTVSLVFIIGAIVVGKQITYMRVADKGFNTDRILTLTSWNSKPEQLREY
                     ANAIRQLPDVAKVILEGAPPMGFAQGADLYADRPAPDANRLVHAEMGDEDYIPFYGMR
                     LVAGRNVFHSDSLRELVINETMAKTMGCRTPKEALGRVLYQVVSQGKLGKGYPVVGVV
                     ADFHEGSFHDPIPPTVIENVKDRQHGVAIQLINADPRTVKAVVAGMREKWKERFPDQP
                     FQAAFLTESIDWLFHQEEDQAWLVNAAMGVTIFISCMGLFGLGLFTVRRRSKEISIRK
                     VLGASVTGITTLLTADFAWLVGLAFCVASPLGWYLARTWLEDFAYRTGLNWWVFAAAG
                     LAALVIAMLTVGFQAAKTAMASPVKYLRNE"
     gene            complement(2193..2864)
                     /locus_tag="VKU83_02945"
     CDS             complement(2193..2864)
                     /locus_tag="VKU83_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92534.1"
                     /translation="MKLLIIEEEKGSSRSILQYLRRDTYRCDVSLEAEYLSQSPALAA
                     FDCILLNCVHSDDMAFRVLHMLNAQDYPGGVIMLSFQRSPESRVRGLNAGADDYLSQP
                     VHFGELSARIGAIIRRRNFAGNKLLVVKELSIDLAGKLVSVRHNPVDLTRKEFDLLLY
                     LASNPNRVVSKESIATNLAEENEPHQSPDFIYAHVKNLKKKLAAAGCRDCIASVYGMG
                     YKFTT"
     gene            3085..>3404
                     /locus_tag="VKU83_02950"
     CDS             3085..>3404
                     /locus_tag="VKU83_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92535.1"
                     /translation="MCKRSFVVWVIFILSVQRGLGQTGEIRGNVYDQVTRDGLSGASI
                     SLEHTAFKRAADAKGNFSLLQIPAGEYELIVTSVGYLEVRRHIRVKRGEVQKIFIALS
                     QATGA"
BASE COUNT          731 a          910 c         1014 g          749 t
ORIGIN      
        1 gaagcatttc gacaaccacg taggcgatca ataccagagc gcggctatta taaccgtccc
       61 ggattatttc cgcgtgtttc cttatcgctg gttggcggga aatgcggcgg tgctcgatcg
      121 gcaggacgag ctggtcctga ccgagagccg gggccggctg tacttcggga atatcccgcc
      181 ggcggagatg gtcggaaaga tggtcgtcta cgatgattcg ctccaggtcc atgtcggcgg
      241 cgtggtgcgg gacctcgcgg gaaataccga catgggttac acggatttca tttcgatcgc
      301 gacggcgacg cacagctttc tgaaagactg gatccctacg gccgactgga agagcctgcg
      361 gccccaccaa agcatggcgt gggtcaaact ggcaaaggga gtgactgccg ggcaggtaaa
      421 tgccagcttt gcggaatata tccggcagca tccacccagg gggcggggaa cgctgaactt
      481 ctacctccag ccgctggaag atattcattt cacccggaat ttccaccggg gagaaggaga
      541 catgggagat gacggcgaca tgtggcgcaa gccctatctg cccgagctgg atgccatgat
      601 cggcgtcgcc atcttcatac tgctgattgc agccatcaat ttcattaacc tgtccactgc
      661 gcagtcaatg agcagagcta aagaagtcgg ggtgcgaaaa gttatgggca gccgggtgtc
      721 gcatctccgc ttccagttcc ttgtcgagac gatgcttgtg gtcctgctcg cagtcatact
      781 ggcggtaagc ctggtgaatc cggtgctgca cctctttcag gcatacctcc cgcgaggggt
      841 gcgtttcgag ctcgcgtatt ccggcacgct ggctttcctg gccgggctca tcctggtgac
      901 cactttgctg gcgggcgcct atcccgcctg ggtcatggcc cgctatcggc ccgtggaaag
      961 cctcaagggg gagatcagcg gcgccggtcc gggcagtcta cacctgcggc gggcgctgat
     1021 cgtgttccaa ttcaccgtct cactcgtatt tatcatcggc gctattgtgg tcggcaagca
     1081 gatcacctac atgcgggtcg cagataaggg cttcaacacg gaccggatat tgacgctgac
     1141 aagttggaac agcaagccgg aacaactgcg ggaatatgcc aatgctatcc gccaattgcc
     1201 ggatgtggcg aaggtcatcc tggagggtgc gccgccaatg ggttttgccc agggtgccga
     1261 cctatatgcg gaccggcctg caccggatgc caatcggctg gtgcatgcgg agatgggcga
     1321 cgaagactat atcccgttct acggcatgag acttgtcgca ggccgcaatg tttttcacag
     1381 cgacagcttg cgggaattgg taataaatga gacgatggcc aaaacaatgg gttgcaggac
     1441 gccaaaggag gcgctcggcc gggtcttgta ccaggtcgtg tcccagggaa agctcggcaa
     1501 gggctacccc gttgtcggcg tggtggccga ctttcacgag ggttccttcc acgatccgat
     1561 cccgccgacg gtcattgaaa atgtgaagga ccgccagcac ggcgtggcga tccagttgat
     1621 caatgcagat ccccggacgg tcaaagcagt ggtggcgggg atgcgcgaaa aatggaagga
     1681 acggtttccg gaccaaccgt tccaggcagc ctttttgacc gagtcgattg actggctgtt
     1741 ccaccaggag gaggaccagg cctggctggt gaacgcggcg atgggcgtca ccatctttat
     1801 ttcctgcatg gggctgttcg gactggggtt gttcacggtc cggcgccggt caaaggaaat
     1861 cagcatcagg aaggttttgg gagcaagtgt aacggggatc acgaccttac ttaccgccga
     1921 ctttgcatgg ctggtcggcc tggcgttctg cgtggcgtcg cctcttggtt ggtatcttgc
     1981 gcgtacctgg ctggaggatt ttgcttaccg gaccggcctg aactggtggg tgtttgcggc
     2041 ggcaggactc gcggcgctgg tcatcgccat gcttaccgtg gggttccagg cggcgaaaac
     2101 cgcgatggcg agcccggtga agtacttgcg gaatgaataa aagcgcccct tcaggccagc
     2161 acgacggtgc ggctccccgg gggcgggacg aatcatgtcg taaacttgta gcccatccca
     2221 taaaccgacg cgatacagtc gcgacatccg gcggccgcaa gcttcttctt cagattctta
     2281 acatgcgcgt aaataaaatc ggggctttga tggggctcgt tctcttccgc caggttggtc
     2341 gcgatgcttt cttttgagac taccctgttg ggattcgacg ccaggtagag cagcagatca
     2401 aattcttttc gggtcaggtc caccgggtta tgacggaccg aaaccaattt acccgcaaga
     2461 tcgatggaga gctccttaac aaccaataat ttgtttcccg caaaattcct tcggcggata
     2521 attgcgccga tccgggcgct caattcacca aaatggaccg gctgtgagag gtagtcatcc
     2581 gctcccgcgt tcaggccgcg aaccctcgat tccggcgaac gctgaaagga aagcatgata
     2641 acgccccccg gataatcttg tgcattcagc atgtgcagca ccctgaaggc catgtcatcc
     2701 gagtgaacgc aattcagcag gatgcagtca aaagcggcca aagccggcga ctgcgagagg
     2761 tactcagcct caagggaaac atcgcagcga tacgtgtccc gccgcaagta ttgcaatatg
     2821 cttcgcgagc tgcctttttc ttcctcgatg atgagaagct tcaaaggacc ggtgtttttt
     2881 ccccaccaat gttacgacag aattttgaaa ttagccggat tgggccaact gcgatcctgg
     2941 cgacattttg aaatcaaaat ggaaaacaag tgcgcgtgtt atcaaatgat taagcaattg
     3001 ttgtcctatg tttatttctc tcccgcgtga acccgcactg cttcatttag caccatattt
     3061 gccccatcaa caatattagc tgctatgtgt aagcgaagtt ttgtcgtgtg ggtcattttt
     3121 attttgtcgg tacaacgggg gctcggccag accggtgaga tcagggggaa cgtgtacgat
     3181 caggtcaccc gtgacggctt gagcggcgct tccatctcgc tggagcacac tgcgttcaaa
     3241 cgggcggccg acgccaaagg aaacttttcc ctgttgcaaa tccccgccgg cgaatatgaa
     3301 cttattgtta ccagtgtcgg atacctggaa gtgaggcggc atattcgtgt gaaaagaggc
     3361 gaggtccaaa aaatctttat cgcattgagt caggcaaccg gcgc
//
LOCUS       DATGKE010000171         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1134287, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000171 DATGKE010000000
VERSION     DATGKE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134287"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VKU83_02955"
     CDS             complement(<1..380)
                     /locus_tag="VKU83_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92536.1"
                     /translation="MKRTAVALLLAVGFLPYHGHAQLTNGGLYALFGVDADTRTNYLK
                     YGTVTGTMASDDWFAPSGSGNNVIDTTNASIYKALLQAGTNTSFSQRMSQLLYAKIGG
                     KLWLDAAYGRDYMATAALKDSTTFA"
     gene            complement(572..1216)
                     /locus_tag="VKU83_02960"
     CDS             complement(572..1216)
                     /locus_tag="VKU83_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI92537.1"
                     /translation="MIIDQTTAISNRASDEHLITRILSGEKQDFEVIIRRYNQRLFRV
                     GMSVLGNEGEVEEAMQAAYLSAYEHLHQFEFRAAFGTWLTRIMLNQCFKARRSQIRSS
                     PLTADSENVINMKTPENVMCSKELNQVLENAIARLPEKYRLIFVLREIEELSVKEAAE
                     VAGIQESNVKVRLNRAKSMLRQSLNHYMRDSVYPFHLSRCNEIAASVMGKILPA"
     gene            1312..1698
                     /locus_tag="VKU83_02965"
     CDS             1312..1698
                     /locus_tag="VKU83_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92538.1"
                     /translation="MATIKQLQGTLKTTFVVVPVVAGLDKFTNLLTQWSAYLDKSLIP
                     FDPAVFMKIVGVVEIVAGLIVLLRPVIGAYIVMVWLICIALQLIAGGHYFDVAVRDLV
                     MAIAAFTLAQLTMMQQAGGRPVREKV"
     gene            1702..2367
                     /locus_tag="VKU83_02970"
     CDS             1702..2367
                     /locus_tag="VKU83_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92539.1"
                     /translation="MSHFCKILSIIPVTHDVRSIRLVKPANYSFVPGQATDVAINKDG
                     WADQERPFTFTSLNSDPYLEFTIKCYNDRHGVTEQIGHLVPGDEMIIGEPWGSIRYKG
                     EGYFIAGGAGITPFLAIFRQLHQVGLIGKNKLFFSNKTAGDIIYRDELIRIFEDKVTF
                     VLTAEGEHQQAIDYIDEKFLQFHVSRYDNCFYVCGPDPMVHAINEILTDAGVSPQSLV
                     FEK"
BASE COUNT          587 a          653 c          645 g          610 t
ORIGIN      
        1 gcgaaggtgg tggagtcttt taaagccgcc gtagccatat aatcccggcc ataagcggca
       61 tccagccaaa gcttaccacc gatcttggca taaagtaact gcgacatccg ctgactgaac
      121 gaggtatttg tcccggcctg caataaggcc ttataaatgg aggcattcgt cgtatcgatc
      181 acattattgc ctgagccgga cggcgcaaac cagtcgtcgc ttgccatcgt cccggtaaca
      241 gttccgtatt taaggtaatt ggtgcgcgta tccgcgtcta cgccaaacaa ggcgtaaagg
      301 cccccgttgg tgagctgggc gtggccgtgg tagggtagga accctacagc gagcagcagg
      361 gcaactgcag tacgtttcat agtaacggat ttagggtaca caggataagg gattagtaaa
      421 aatacggttc attgcgtaaa aatgcaagtc cagcggccgg attcgccaga cttccgaccc
      481 taaatcactt aaaatcagcc gggtaaaaat tatttttttt gagaccccaa ccgggaagcc
      541 cgattttggc gcacgctgag cgctgataat gtcaggcggg aagaatcttg cccattactg
      601 aggccgctat ttcattgcac cggctaagat gaaatgggta aaccgagtcc cgcatgtaat
      661 ggttgaggct ttgacgcaac atactttttg cccggtttaa gcggactttg acgttggatt
      721 cctggattcc ggcaacctca gccgcctctt ttaccgaaag ttcttcaatc tcccttaata
      781 cgaagattaa ccggtatttc tccggcaacc gcgcgatcgc attttccagc acctggttca
      841 attctttgct gcacattaca ttttccggcg ttttcatgtt gatgacattt tcgctgtccg
      901 ctgtgagagg tgaagaccgg atctgactac gccttgcctt gaaacattga ttgagcatga
      961 tccgggtgag ccaggtaccg aaagcagccc ggaattcgaa ttggtgaagg tgctcatagg
     1021 cactgaggta ggcagcctgc atcgcttcct cgacctctcc ctcatttccc agtacggaca
     1081 ttccgacccg gaagagccgc tgattataac gccggataat cacttcgaaa tcctgttttt
     1141 ctccagaaag gattcgcgtg atgagatgct cgtccgacgc ccggttcgat atggccgttg
     1201 tttgatcgat gatcattgga ttggcgccgg gctaaaaggt tacgcagcaa tcgtcctttt
     1261 ttgtaaccat tcggcgcatt tttgttccaa tatggaatta aaaccaaatt tatggcaaca
     1321 attaaacaac tccaaggcac ccttaagacg acattcgtcg ttgtcccggt cgtggccggc
     1381 ctggacaaat tcacgaacct gctgacccaa tggtcggcct atctcgacaa gagcctgatc
     1441 ccatttgacc cagcagtctt tatgaaaatc gtcggagtcg tcgagatcgt agccggcctc
     1501 atcgtgctgc tgcggccggt tattggcgcc tatatcgtga tggtatggct gatttgcata
     1561 gctttgcagc taatcgccgg tggccattat ttcgacgtgg ctgtcaggga cctggtgatg
     1621 gctattgcag cttttaccct tgcccagctt acgatgatgc agcaggcagg cggtcgcccg
     1681 gtccgggaaa aagtttaagt tatgtcccat ttctgcaaaa ttctttcaat tattccggtt
     1741 acccacgacg tccgttccat ccggctggta aagcccgcca actactcttt cgtccccggg
     1801 caggccacgg acgtcgccat caacaaagac ggatgggcgg accaggaacg gccttttacc
     1861 tttacgagcc tcaacagcga cccgtatctc gaattcacga tcaaatgcta taatgaccgc
     1921 catggcgtga cggaacaaat cggccacctg gttccgggag atgaaatgat aatcggggag
     1981 ccctggggtt cgatccgata caagggtgag gggtatttta ttgccggcgg cgcaggcatt
     2041 acgccttttc tcgccatttt ccggcagctc caccaggtgg gcctgatcgg caagaacaag
     2101 ctcttttttt cgaacaagac cgccggggat atcatctaca gggacgaact gattaggata
     2161 tttgaggata aggtgacgtt tgtcctgacg gcggaaggcg agcaccagca ggccatcgac
     2221 tatatcgacg agaaattcct tcagttccac gtttcccgtt atgacaactg cttttatgtc
     2281 tgcggcccgg accccatggt gcacgcgatc aacgaaatcc taacggacgc gggagttagc
     2341 ccgcagtcgc tcgtcttcga aaaatgaagc accttcgcac cggtcacctc gcgcctttga
     2401 ccggatagcc gcgatcgatc cagtctgcgc ggatattatg ggaaagattg agcagccggg
     2461 catgcgtaaa atgcatcgac atgagcaggg cgaag
//
LOCUS       DATGKE010000172         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_684260, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000172 DATGKE010000000
VERSION     DATGKE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684260"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1373
                     /gene="lysA"
                     /locus_tag="VKU83_02975"
     CDS             162..1373
                     /gene="lysA"
                     /locus_tag="VKU83_02975"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLI92540.1"
                     /translation="MSQILTSDQLSALAQEFGTPLYVYHAEKIKEQYQKLTAAFSGSD
                     TVFFYACKALTNINILRYILELGANVDCSSTNEVRLALHAGFPKERILYTSNGIGFDE
                     IEEAQALGVIINIDSLSNLEKFGRRFGSSYPVGVRLRPNIMAGGHLKMSTGHDKSKFG
                     IPVDQADKILGLVKQFDLRVHDLHIHTGSEIKDVDVFVQGIDILFDLIPRFNDLRFID
                     LGGGFKVPYRDGDSETDIPALARKVREAFASHPNPGGRPLQVWFEPGKFLVSECGYFI
                     TKVNVIKQTAAATFVSVDSGFNHLIRPMFYDAYHRIVNISNPAGGHQRYSVVGNICET
                     DTFAWDRSIAEVREGDYLVFYNAGAYGFEMSSNFNSRYKPAEVLVRDGQPRLIRRRDS
                     FADLLRNQIES"
     gene            1386..1862
                     /locus_tag="VKU83_02980"
     CDS             1386..1862
                     /locus_tag="VKU83_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HLI92541.1"
                     /translation="MAEFDRLARSALRFRLFLLTRMPSAFFSGLHITRLDADSCVVSV
                     PYTWFTRNPFRSTYFACLAMAAEMSTGILAISNVYKRKPPVSMLVVHLEANYFKKATG
                     PTHFHCKDGPGIRETIERAIATGDGQTIRARSEGANAAGEPIAEFFITWSFKPKKS"
     gene            1916..3319
                     /locus_tag="VKU83_02985"
     CDS             1916..3319
                     /locus_tag="VKU83_02985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLI92542.1"
                     /translation="MKIAFHGAARVVTGSKHLLSLNNGKKYLLDCGMFQGLGPQTDTL
                     NRNWGFTPAQVDHLILSHAHIDHSGLIPKLIKDGFTGKIYCTPATRALTGILLQDSAE
                     IQEDEVKFANKRRKAEGQPYLQPLYTVEDAKLVEGHFVSVEYDTWYTIDENVQVLYTD
                     AGHIIGSAAVSLKIKEDGKETRLTFSGDVGRYKDVILRSPEVFPQADYILIESTYGNS
                     LHDPAGTTPDMMLEWINRTCLQKKGKLIIPAFSVGRTQELLFALNQLEEEHRLPALDY
                     YVDSPLSVAATEIVKSYPQYFNKTIQKVLETDKDPFMFEGLKYIKTVEESKLLNYHDK
                     PCVIISASGMADAGRVKHHISNNIENSRNTILLVGYCEPHSLGGRLMAGAKEVHIFGV
                     LHEVHAEVGSIRSMSAHGDYEDLCQFLACQDTRQVKTVFIVHGEYDVQQAFRDRLIKK
                     GFTDVEIPELHFEKGLT"
     gene            complement(3316..>3813)
                     /locus_tag="VKU83_02990"
     CDS             complement(3316..>3813)
                     /locus_tag="VKU83_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLI92543.1"
                     /translation="LQEQRGLVDTYLAIRHDPAFRLRVTHHDTKISNVLFDSSDRGLC
                     VIDLDTVMPGYFISDVGDMIRTYVSPASEEEADFDKVLVRGEIYQAIEAGYMEEMGDV
                     LTARERGHFLYGGLYMVYMQALRFLADHLNRDVYYGAKYQGQNFVRAGNQVSLLRKLI
                     EFGAR"
BASE COUNT          878 a         1167 c          962 g          806 t
ORIGIN      
        1 cgacaattgt ccaagccgaa ggtgccgaag ccgaagaaac ccggggcgaa ggcagccccc
       61 ccgaccagcc gcagcgcgat gagccgtcag cgggcgaggc tccggctgat caagaccggc
      121 aaacccaaga aacctgacga acctctacaa taacgcacga catgtcgcag atcctcactt
      181 cggaccaact ctccgccctc gcacaggaat ttggtacccc gctctatgtt taccacgccg
      241 agaagataaa ggagcagtat caaaaactaa ccgccgcctt cagcggcagc gacacggtct
      301 ttttctatgc ctgcaaagcg ctcactaaca tcaatatcct ccgctacatc cttgagcttg
      361 gcgcgaatgt ggattgcagc tctacaaacg aggtgcgcct ggcgctccat gccggctttc
      421 cgaaagagcg cattctttac acctctaacg gcatcggctt tgacgagatc gaagaagcgc
      481 aggccctggg ggtcatcatc aatatcgaca gcctctccaa cctcgagaaa tttggccggc
      541 gctttggctc ctcctatccc gttggggtgc ggctgcgccc caatatcatg gcggggggcc
      601 acctgaaaat gtcgaccggc cacgataaat cgaaattcgg cattcccgtg gaccaggcgg
      661 ataagatcct cggtctcgtg aagcaattcg atctcagggt gcacgacctc catatccata
      721 ccggttcgga gataaaggac gtggatgtct tcgtacaagg catcgacatt ctttttgacc
      781 ttattccaag gttcaatgac cttcgattca ttgaccttgg cggtggcttc aaggttccct
      841 accgggatgg cgactcggaa accgatatcc cggcgctcgc gcgaaaggtc agggaggcct
      901 tcgcctctca tcccaatccg ggtggccgtc cactccaggt ctggttcgag cccggcaagt
      961 tcctcgtcag cgagtgcggg tattttatca cgaaagtgaa cgttatcaag caaaccgcgg
     1021 ccgcgacgtt tgtgagcgtc gacagtgggt tcaaccactt gatccggccg atgttctacg
     1081 acgcctacca ccggatcgtc aatatcagca acccggccgg cgggcaccaa cgctattccg
     1141 tcgtcggcaa tatctgcgaa acggatactt tcgcctggga ccgcagcatc gccgaggtcc
     1201 gcgaaggcga ttatctcgtt ttttataacg ccggcgccta cggctttgaa atgtcatcca
     1261 atttcaactc gcggtataag cccgctgaag tcctcgtcag ggatggtcag ccccggctta
     1321 tccggcggcg cgacagcttt gcggacctgc ttcgaaacca gattgaatcg taaaatattg
     1381 ttcctatggc tgagtttgat cgccttgcgc gcagcgccct gcgtttccgc ctcttcctgc
     1441 taacgcggat gcccagcgct tttttctcgg gactgcacat cacgcggctg gacgccgatt
     1501 cctgtgtcgt gagcgttccc tatacctggt ttacccgcaa tcccttccgc tcgacttatt
     1561 ttgcctgcct tgccatggcg gcggaaatga gcaccggcat ccttgcgatt tcgaacgtct
     1621 acaaacgaaa gcctcccgtc tccatgctgg tggttcacct cgaagcgaac tatttcaaga
     1681 aagctaccgg cccgacccat tttcattgca aggacggccc aggcattcgt gaaacgatcg
     1741 aacgggccat cgccaccggc gacggccaaa ccatccgcgc ccgctccgag ggcgccaacg
     1801 ccgccggcga acccatcgcc gaatttttta tcacctggtc cttcaaaccc aaaaagtcgt
     1861 aaattgacgg cagctgcggc tttacctgcc gcaaattcat cccaacgcaa agactatgaa
     1921 gatcgcattc cacggcgccg cacgcgtcgt caccggctcg aagcatctcc tgtccctcaa
     1981 taacggcaag aagtacctgc tggactgcgg gatgttccag ggcctcggcc cccagaccga
     2041 taccctcaac cgcaactggg gcttcacgcc ggcgcaggtg gatcatctta tcctgtcaca
     2101 tgcgcatatc gaccacagcg gcctcatccc aaaactcatc aaggatggtt tcacgggcaa
     2161 gatctactgc acccccgcca cccgcgccct taccggcatt ttgctccagg actccgctga
     2221 gatccaggag gacgaggtca agttcgccaa caagcgccgg aaggccgagg gccagcccta
     2281 cctccagcca ctctataccg tggaggatgc gaagctggtc gaagggcatt ttgtgagcgt
     2341 ggaatacgat acgtggtata cgatagacga gaacgtccag gtcctctata ccgatgccgg
     2401 ccatatcatt ggcagcgccg ctgtcagtct taagataaag gaagacggca aggagacgcg
     2461 cctaaccttt agcggtgacg tcggccgtta taaggatgtc atcctgcgtt ccccggaagt
     2521 ctttcctcag gcggattata ttctcatcga gtctacttat ggtaacagcc tgcatgaccc
     2581 ggccggcacg acccccgata tgatgctgga atggatcaac aggacctgtc tgcaaaaaaa
     2641 agggaaactt atcattcccg cttttagcgt cggccgcacc caggagttgc tttttgcgct
     2701 caaccagctg gaagaggaac accggttgcc tgcccttgat tattatgtcg acagtccgct
     2761 aagcgtggcg gctaccgaga tcgtcaagag ctatccgcag tacttcaaca agacgatcca
     2821 aaaggtcctt gaaaccgaca aagacccctt catgttcgag gggctcaaat acatcaaaac
     2881 ggtagaagaa tccaagcttc tcaactacca cgacaaaccc tgcgtgatca tctcggccag
     2941 cggaatggct gacgccggac gggttaaaca ccatatcagc aataatatcg agaacagcag
     3001 gaacaccatc ctcctggtcg gctactgtga accgcattcc cttggcggcc gccttatggc
     3061 cggcgcgaag gaagtgcata ttttcggggt cctgcatgaa gtccacgccg aggttggcag
     3121 catccgcagc atgagcgccc acggcgacta cgaagacctc tgccagttcc tggcgtgcca
     3181 ggacaccaga caggtgaaaa ctgtctttat tgtccatggg gaatatgatg tccagcaagc
     3241 cttccgcgac cgtctcatca aaaaaggatt caccgacgtc gagatccccg aattacattt
     3301 tgaaaagggc cttacttagc gtgctccgaa ttctatcagc ttcctgagca aactgacctg
     3361 gttgcccgcg cggacaaagt tctgcccttg atactttgct ccataataca catccctgtt
     3421 caaatgatcc gccaggaacc ggagcgcctg catgtagacc atgtagaggc cgccgtaaag
     3481 gaaatgccca cgctccctgg ccgtgagcac atctcccatc tcttccatat atcccgcttc
     3541 gatcgcctga taaatttctc ccctgaccaa caccttatcg aaatcggcct cttcttcgct
     3601 tgcgggggac acgtaggtcc tgatcatatc tccgacatcg ctgataaaat atcccggcat
     3661 gaccgtatcg aggtcaatga cgcaaagtcc cctgtcggaa gaatcgaaga ggacgttgct
     3721 gatcttcgta tcgtgatggg ttacccgaag gcggaacgcg ggatcgtgcc gtattgccag
     3781 gtaggtgtcg accaagcccc gctgctcctg caa
//
LOCUS       DATGKE010000173         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1201324, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000173 DATGKE010000000
VERSION     DATGKE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201324"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VKU83_02995"
     CDS             <1..823
                     /locus_tag="VKU83_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92544.1"
                     /translation="QPQPPVVLITDSQAMPPPVPPAAAMPQQQAPPLSCPALPIYGDT
                     IVYPQPANGGDYIISPINNPGAGTYLSWPAGMVMDSTTGAIDLTASETGLKYMIGYVR
                     SGTTDTCLSTLVIGGASYADSVYVLSEGETTAVPYFEANPADLNLCQTSNACHFDVTG
                     SAARMKVIVNTSNGQIDLQKTLNGTGLLGGAFGLLPVNGQTITAPIYYQIGDDPSNNA
                     LQNISVQLVYYNNASLINIGLLNTVVNKLDNLLSGNQISMTSNPRPPLIVIVRSK"
     gene            950..1900
                     /locus_tag="VKU83_03000"
     CDS             950..1900
                     /locus_tag="VKU83_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92545.1"
                     /translation="MKTSPTQFLWTALVFIFSSCQKDGGSIQHFVVGQIHNPPVQDSI
                     FQPPVVLITDSQQMAPPILPRAPLPQQPAPALSCPALPIYGDSIVYAQPANGGDFIIQ
                     PVNNPGPGTYLSWPVGMVIDSATGAIDLTASQSGMKYIVGFVKSGSTDTCLSTLIIGG
                     ASYADSVYILNNNRKVAGPYFEGNPANVSLCQTANACTFDVTGSAGAHGVVINPHNGQ
                     IDLLKTLKGVANQGGAFGPAPVNGQTITADFYYQINDDPSNNAVQHTSLQFVYYDNEA
                     LINAGLLNTIVTRLDNLVSGNLISTTANPKPPLIVIVRHN"
     gene            2017..>2592
                     /locus_tag="VKU83_03005"
     CDS             2017..>2592
                     /locus_tag="VKU83_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLI92546.1"
                     /translation="MGLFPLLGNSGDPVSRIAAIYRRADSLFELTNNSRYTDSLALAG
                     FVKVIDGVQRMPPGVARDTLLFQSWLKKGILLDSRFDYSGAKAAYCQALHVHPKEDSL
                     TFVLWVYAGASYYNLNDFDSARYYLRNAESRSGRYADPADNVRLYNTLGVLFYDNGNY
                     EQGKNYFNHALSIVQGRRPFDTAAAVSLQTNI"
BASE COUNT          597 a          752 c          682 g          561 t
ORIGIN      
        1 gcagccacag ccgccggtcg tgctcatcac ggacagccag gccatgccgc cgccagtgcc
       61 gcccgccgcg gcgatgccgc agcagcaagc tcctccattg agttgccccg cgctgcccat
      121 ttatggggat accatcgtat atccgcaacc ggccaatggt ggcgattaca ttatttcgcc
      181 gatcaataac ccgggcgccg gcacctattt gtcctggccg gcagggatgg tcatggattc
      241 cacaacgggc gcgatcgacc tgaccgccag tgaaacgggg ttgaaatata tgattggcta
      301 tgttagaagc gggaccacgg atacctgtct aagtaccttg gtcatcggcg gcgcttcgta
      361 cgcggatagc gtctatgtgc tgagcgaggg ggaaaccacc gcggttccct attttgaagc
      421 aaacccagcg gatttgaacc tctgtcagac ttcgaacgcc tgccatttcg acgtcaccgg
      481 ttcggccgcc aggatgaagg tcatcgttaa taccagtaac ggccagatag acttgcagaa
      541 gaccctgaac ggcactggct tgctgggcgg cgccttcggg ctgctgccgg taaatggcca
      601 gaccattaca gcgccgattt actaccagat cggggatgac ccaagcaaca acgccctgca
      661 gaacatctcg gtacagttgg tatattacaa taacgcgtcg ctgataaata tcggcttgtt
      721 gaatactgta gtgaacaagc tggataacct gctcagcggg aaccagatca gcatgaccag
      781 caacccgaga ccgccattga tcgtgatcgt gcggagtaaa tagtggcact agctgaaatt
      841 caatgtgttg gcccgaaata tttctttagg gaaatggatg ctgaggtttg aacatataaa
      901 aaattctttg tatcttgttt gtgaccctta gcatccaaaa cccttgaaaa tgaaaacaag
      961 tcctactcaa ttcctatgga ctgcgctagt attcattttt agttcctgcc agaaagacgg
     1021 cggttccatt caacattttg ttgtcggaca gatccataac ccgccggttc aggattcgat
     1081 tttccagccc ccggtcgtcc tgatcaccga cagccagcag atggctccgc cgatcttgcc
     1141 cagggcgccc ttgccgcaac aaccggctcc ggccctcagt tgtccggctc tcccgatcta
     1201 cggtgactcg atcgtgtacg cacaacctgc caatgggggt gactttatca tccaacccgt
     1261 caacaatccc ggcccgggca cctatctttc ctggccggtg ggaatggtga tcgactccgc
     1321 cacgggggca atcgacctga cggcaagcca gtcgggaatg aaatacatcg ttggcttcgt
     1381 gaagagcgga agtacggaca cttgtttgag cactttgatc atcggtggcg cctcatacgc
     1441 cgacagcgtg tacattctta acaataacag gaaggttgcc gggccttatt tcgagggcaa
     1501 tcccgccaat gtcagccttt gccagaccgc gaacgcctgt acgtttgacg tcacaggttc
     1561 agccggcgcc cacggcgtcg tgatcaaccc gcacaatggg caaatcgacc tgttgaagac
     1621 cctgaagggg gtcgctaacc agggcggtgc attcggcccg gcgccggtga acggccagac
     1681 gataacggcc gacttctact accagattaa cgacgaccca agtaataatg ccgtgcagca
     1741 tacctccctg cagttcgttt attacgacaa tgaggcgctg atcaatgcgg ggttgctgaa
     1801 taccattgtg accaggctgg ataacctggt cagcggcaac ctcatcagca cgacagccaa
     1861 cccgaagccg ccgctcatcg tgatcgtcag acataattaa accgtgaacc cgacgtcatg
     1921 accgcagcga ccggcagcgc ccgcatacac aattaatgcg ccgccggggc cgttgtagtg
     1981 ggcatggtta agcggttaat gacagctgcc ctgtttatgg gtctctttcc gctgttaggc
     2041 aattccggcg accctgtgag ccggattgcc gctatatacc gccgcgcaga cagcctcttc
     2101 gagctcacca ataattcccg gtacaccgac agcctcgcgc ttgccggctt cgtcaaagtc
     2161 atcgacggcg ttcagcgcat gccgccagga gtggccaggg acacgttgct ttttcaatcc
     2221 tggctgaaaa agggcattct actggattcc cggtttgact attcgggggc caaagcggct
     2281 tactgccagg cccttcatgt ccacccgaaa gaggattccc tgaccttcgt cctttgggtc
     2341 tacgcgggcg ccagctatta taacctgaac gatttcgatt cggcgcgcta ctatctccgg
     2401 aatgctgaat cgcgaagcgg ccggtacgcg gatccggcag acaatgtaag actctataac
     2461 accctgggcg tactgttcta cgacaatgga aattatgagc agggaaagaa ttatttcaac
     2521 catgccctca gcatcgtcca gggccggcga ccattcgata ccgcagccgc ggtaagtcta
     2581 cagaccaata tc
//
LOCUS       DATGKE010000174         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1202343, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000174 DATGKE010000000
VERSION     DATGKE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202343"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..141
                     /locus_tag="VKU83_03010"
                     /pseudo
     CDS             1..141
                     /locus_tag="VKU83_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            complement(297..875)
                     /locus_tag="VKU83_03015"
     CDS             complement(297..875)
                     /locus_tag="VKU83_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92547.1"
                     /translation="MTKIWSLGFALFFGLGFASAQSSFRFRSSESLGISNGQLGGFVQ
                     VQAINGIAYGPWFLGAGAGLDYYRFRSVPLFLSAGRDLWLTKKEGLGLFLNGGTNLPW
                     LQGGGSPNTGTITLSGGEYWSGGISYILRLGQHTREAVFFSVGYEVKKMGEHAVWKTL
                     SPCYNMAGCPIDQTVDYQYLNRILFLAAGYRF"
     gene            complement(872..2170)
                     /locus_tag="VKU83_03020"
     CDS             complement(872..2170)
                     /locus_tag="VKU83_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92548.1"
                     /translation="MRKEIKVATAPLLLPTLLLAAAGLFELQGCLKDRAMETYKIYTP
                     VYVRRDTVLHKIVGNAAAPVSRPGQIYVKGNYIYLNDIDKGIHIFDNTNPSNPVQTGF
                     LLIPGNENIAIRNNILYADMYTDLVSIDISDPRNPRVVGTLQNFFTGRNTGANPGYML
                     SGWIVRDTTCPVPPGGTGIFLIPHSNYLTYFGAAVPAAAFAANSANSSGNTGIAGSEA
                     TMTLVGNYLYAIPEQHSLGVVDISDSSHPALVQSSFAGDDLETIFPLQDKVLLLGSKE
                     GVFVYSISNPAQPTQVSEFTHGTACDPVIADPKFAYVTLRSGTDCGGAANELDVLDAQ
                     DIMNTALLKSYPMTSPSGLCEDGSLLFVCDGTVVKVFDNSDPQNLQLLSSLPVTRPND
                     VIAANHILIVVAASGLYEFDYTDPKNVVQKSFIATTNLNS"
BASE COUNT          508 a          703 c          665 g          517 t
ORIGIN      
        1 ctcgccgcgg gctgtatccc gggcggcacg gagcggaact gggcaagcta cggaaagcgg
       61 gttcgcctct caacggaaga gcagcgccag cttctcgccg acccgcaaac gagcggtggc
      121 ctcctggtcg ccgtcaatcc cgcgagttgc attgctgtcg aagccctcct gtatcagcat
      181 catgtcccgt ccgaatgctg ccgttccttt gggcaactgc gcccccgcga catccctgaa
      241 gcaccgctca tcgacgtcca taccctcagg ggaagctgac tttacccgcg ccctgctcag
      301 aacctatagc ctgccgccag gaacaatatc ctgttcaagt actgataatc gactgtctga
      361 tcgatcgggc aaccggccat gttgtagcag ggggacaggg tcttccatac ggcgtgctcg
      421 cccatttttt tcacttcata ccctacggaa aagaaaactg cttcccgggt atgctgtccc
      481 agtctcaaaa tatagctgat gccgccgctc cagtattctc cgccggagag ggtgatcgtg
      541 ccggtgttag gcgaaccgcc gccctgcagc cagggaaggt tggtcccgcc gttcaggaac
      601 aggccaagcc cctccttttt ggtaagccac aggtcccggc cggccgacag gaaaaggggt
      661 acgctgcgga accggtagta atccaatccc gctccggcgc caaggaacca tggaccgtag
      721 gcgatgccat tgatcgcctg cacctggaca aaccctccca gttgcccgtt gctgatcccc
      781 agcgactcgc tggaacggaa cctgaaggat gactgcgccg acgcaaaccc cagcccgaaa
      841 aacaaggcaa agccaagcga ccatatcttc gtcatgaatt gaggttggta gtggcaataa
      901 aactcttctg cacgacattc ttcggatcgg tataatcaaa ctcatacaag ccggacgccg
      961 ctacaacgat caggatatga ttagccgcaa tcacgtcatt cgggcgggtc accggcaggc
     1021 tgctcaataa ttgcaggttt tgggggtcgc tgttgtcgaa cactttcact accgttccgt
     1081 cgcacacgaa gagcagcgaa ccgtcctcac acagccctga ggggctcgtc atcggatagc
     1141 tcttgagcag ggccgtattc attatgtcct gcgcatccag cacatccagt tcgtttgccg
     1201 ctccgccgca gtcggtgccc gaacgcaggg tcacgtaggc aaactttggg tcggcaatca
     1261 ccggatcaca agccgtcccg tgggtgaatt cgcttacctg cgtgggttgg gcgggattgc
     1321 tgatcgagta cacaaagacg ccttcttttg acccgaggag taaaaccttg tcctgcagcg
     1381 gaaagatggt ttccaggtcg tctcccgcga aagaagactg gaccagggcg ggatgagaag
     1441 aatcggagat atcgacgacc ccgagactat gttgttcggg aatcgcgtag agatagttcc
     1501 ctaccagtgt catcgtcgcc tccgaacccg cgatgcccgt atttcccgat gaattggcgg
     1561 aattggccgc aaaggcggcg gcgggcaccg cggcgccgaa atacgtcagg tagttcgagt
     1621 gaggaatcag gaagatcccg gttcctcccg gcgggaccgg gcaggtcgta tcccggacga
     1681 tccagccgct gagcatatag cccggattgg cgccggtgtt gcgtcccgtg aagaagttct
     1741 gcaacgtgcc aaccaccctc gggttcctcg ggtcactgat gtcaatggac accaggtcgg
     1801 tatacatgtc cgcatagagg atattattgc ggatggcgat gttttcatta ccgggtatca
     1861 ggaggaagcc cgtctgcacc ggattggacg gattggtatt gtcgaaaata tggatccctt
     1921 tatcgatatc gttgagatag atataattcc ctttcacgta gatctgtccc ggccggctga
     1981 cgggtgcggc ggcatttccc acgatcttat gcaggacggt gtcccttcga acatagacgg
     2041 gcgtatagat cttgtaggtc tccatggcgc ggtctttcag acagccctgc aattcgaaca
     2101 ggcccgcggc cgcaagcagc agcgttggca acaataaggg cgctgtcgcc accttgattt
     2161 cctttctcat agtcggcgtt ttacaccctg acaaggcaac tcgtccggtc gttgcacagt
     2221 gggagggata tactgcatct attaattggg ccgatttcgg cccaattaac cggcacgcca
     2281 cctggccggc cccggagcgc gcttgcctag cctatggcgc agccaggtta tccccaaaat
     2341 attgtcgttt atcccccggg gtctttttgc aagttttttt tccctagttt tca
//
LOCUS       DATGKE010000175         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_62735, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000175 DATGKE010000000
VERSION     DATGKE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62735"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VKU83_03025"
     CDS             <1..876
                     /locus_tag="VKU83_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLI92549.1"
                     /translation="VLDLTGPIQVFYEANAYGADYELDYIALEPNPVSAAGLPFGQIT
                     HFSEISLQEGDYLILPGAHMDYLRSESFKAHKEFFAWLRSLPGKGVLLCSVCTGAFIL
                     AEAGLLDNTRCTTHWKRLEELQLTYPNVITEQNILYTNTDNKYTSAGITSGIDLSLAI
                     IEEHYGPLVTHRVARELVVYHRRSQNHSQKSVYLDYRNHINPGVHAAQDWLIENLQQK
                     TTVEKLAKQVNMSSRNLTRAFKQATGITINEYMKKLRLEKAATLANNPAYSSEYIAGQ
                     VGYADGRQLRRIKKT"
     gene            880..2376
                     /locus_tag="VKU83_03030"
     CDS             880..2376
                     /locus_tag="VKU83_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLI92550.1"
                     /translation="MKKSILPSLLLVLLHFALFGQPLISATKQFYYCTPCGNTRCDTV
                     RYDHPGTCPVCHMALIKKAIDGPFVAKINNEFIGHWGGYAKTTNQQLTFSLAVSADPV
                     VGVLFTATDLNAMNIRGQHVSLYADSLHFELIGDDGSMKFSGILRDGRISGAFIADDQ
                     SVAPENRIGSFELQKVQIQPVNYTVQEVTFRNKEVTLAGSLYIPNGPGKFPAIVCNHS
                     SGDKPRYDGAFMADYLARRGIAVLIYDKRGDGQSTGDWKTSTFEDLADDCIAGIGLLK
                     SNPRINPRLIGIFGHSQGATISPIIVSRSPDVAFNVAAAGFAVTPPEQDVFRVTNILR
                     HQAHFNDKVIDSAIQFYKLWLEVARTGKGWMQMEEAKQKAENTKWYGWVEPPPADSWP
                     WKWYLSAGNYNYAPYWKKVKIPTLLIYGENDEVTPVKPSVAIIETALKEAGNRRYKLI
                     IMPNAIHYFAQVKKEGDLWTKSTPGYFENIYNWIAEVCSIPPASGKAD"
     gene            complement(2524..2597)
                     /locus_tag="VKU83_03035"
     tRNA            complement(2524..2597)
                     /locus_tag="VKU83_03035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2561..2563),aa:Val,seq:gac)
BASE COUNT          742 a          630 c          664 g          647 t
ORIGIN      
        1 gttttggatc taaccggccc tattcaggtt ttttacgaag ccaacgctta cggtgcagat
       61 tatgaacttg attatatcgc actggaacca aatccggtaa gtgcggcagg cttgccgttt
      121 ggtcagataa ctcatttttc agaaatcagc ctccaggagg gagactacct tattttgccg
      181 ggtgcgcaca tggattattt acgttcagag tcgtttaaag cacacaagga atttttcgct
      241 tggcttcggt cattgcccgg gaaaggcgtt ttattatgct cggtttgtac aggagcgttt
      301 attttggccg aagccggttt gctggataat acgcgttgca ctacgcattg gaaacggttg
      361 gaagaacttc agttaaccta cccgaatgtt atcaccgaac aaaatatcct atacacaaac
      421 accgataata aatataccag tgccggtata acttccggca tcgatctttc cctagccatc
      481 atcgaggaac attatggtcc tttggttacg catagggtcg caagggagct ggtggtgtat
      541 catagaagga gtcaaaacca ttcgcagaag agtgtctacc tcgattaccg gaaccacatc
      601 aacccgggtg tacacgccgc gcaggattgg ctgatagaaa atttgcagca aaaaaccacc
      661 gtggagaagt tggcaaagca ggtgaatatg agttcaagaa atttgacgcg cgccttcaaa
      721 caggctaccg ggattacgat caatgagtat atgaagaagc ttaggcttga aaaggcggct
      781 accctggcta acaatcccgc atacagttcg gaatacatcg cgggccaggt cggatacgct
      841 gacggccggc aattaaggcg aattaaaaaa acttgaatca tgaaaaaaag tatccttccg
      901 tcgctccttt tagtcttgct tcattttgca ttgttcggcc agccgttaat ttcagcaacg
      961 aagcagtttt attattgtac gccgtgtggc aatacaaggt gcgacaccgt tcgatatgac
     1021 catccgggaa cctgcccggt ttgccatatg gcgctaataa aaaaggcaat tgatggaccg
     1081 ttcgttgcga aaatcaacaa cgaatttata gggcactggg gagggtatgc taaaacaacc
     1141 aatcagcagt tgacattttc cttggcggtt tcggcggacc cggttgtagg tgtgctgttt
     1201 acggcgacgg acctgaacgc tatgaatatc agggggcagc atgtctcact ctatgctgac
     1261 agcctgcatt ttgaattgat aggcgacgat ggatcgatga aattcagcgg tatattaagg
     1321 gacggtcgaa tatcgggagc ttttattgcg gacgaccagt cggttgcacc tgaaaaccga
     1381 attggttcat tcgagttgca aaaagtgcag atacaaccgg ttaattatac ggtccaggaa
     1441 gttacctttc gcaataagga agtgacgctt gccggtagcc tgtatatacc gaacggcccc
     1501 ggcaagttcc ctgcaattgt ctgtaatcat agcagtggcg ataaaccgag gtatgacggt
     1561 gcttttatgg cagactatct cgctcggcgt ggaatagccg tgctcatcta cgataaacga
     1621 ggcgacggac agtctaccgg cgattggaaa acgtccactt ttgaagacct ggcagatgat
     1681 tgtatcgccg gtatcggcct gctcaaatca aaccccagga tcaaccccag gctgatcgga
     1741 atattcggcc acagccaggg cgccacaatt tctccgatta ttgttagccg aagcccggat
     1801 gtcgccttta acgttgccgc agcaggcttt gcagttaccc cacccgaaca agacgttttc
     1861 agggtaacca atattttacg acatcaggcc catttcaatg ataaagtaat tgacagcgct
     1921 atccaatttt acaagttgtg gctggaagtg gccagaacgg gtaaaggctg gatgcagatg
     1981 gaagaggcga aacaaaaggc agaaaatacg aaatggtacg gatgggtaga gcctccaccc
     2041 gcagacagtt ggccgtggaa atggtactta agcgcgggaa attacaatta cgcgccctat
     2101 tggaaaaagg ttaagatacc gaccttactg atttacggcg aaaatgatga agtgacgcct
     2161 gtgaaacctt cagtagccat aattgaaaca gcgctgaagg aggccggcaa caggcgatat
     2221 aaattgatca ttatgccaaa tgcgatacat tactttgcgc aagtaaaaaa ggaaggcgac
     2281 ctgtggacaa aaagcactcc gggctatttt gaaaatattt ataactggat agcagaagtg
     2341 tgttccatac cgcctgcatc cggaaaagca gattaagcca acgaataccg gttatacgtg
     2401 ttcgcctctt acccgaggcc ccgccgcccg cttcgcgggc tgggcttttt cacgtgtccg
     2461 cttggcaagc aagctcgccc gctcccacat gaaaaagccc ctcgacttcg tcgaggggcc
     2521 tcgtgggtcc aactggattc gaaccagcga cctcttccat gtcaaggaag cgctctaacc
     2581 aactgagcta aagacccttt caaaagatcc ggaaacgctc tggtccggga ctgcaaattt
     2641 tagtcaaatt cccctattta ccaaaatcca tttcatatcc ccc
//
LOCUS       DATGKE010000176         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_478697, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000176 DATGKE010000000
VERSION     DATGKE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478697"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="tilS"
                     /locus_tag="VKU83_03040"
     CDS             <1..782
                     /gene="tilS"
                     /locus_tag="VKU83_03040"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLI92551.1"
                     /translation="NYTRNFFRHRILPLVREAYPAALQNLAANIDRFRGIELLYTQAV
                     ELQKKKLLEYRGGEVHIPVGRLKKATPLITLLYEIFSPYGFTPQQAPAIGSLLESDTG
                     KYICSATHRVLRNRSWLILSPLESMKATTILIESGESAVEFAQGCLRFKRTIPASPPP
                     ADAMVAWLDAKEIIFPLILRPWQPGDYFYPLGMRKKKKLARFFIDQKLSATEKEKIWV
                     LEMNKKIIWVVGRRIDHRFRITPSTKDAWQISFRSAAGSTR"
     gene            complement(754..1371)
                     /locus_tag="VKU83_03045"
     CDS             complement(754..1371)
                     /locus_tag="VKU83_03045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLI92552.1"
                     /translation="MSQSITMIIIILTTIISILSFNNSRLMDALIFWPPAVHLRHQYY
                     RFVTCGFIHADLMHLAFNMFTLYFFGRALENYYMGILGLQHYYFTILYITALIVANIP
                     SYFKQRDNYNYRSLGASGAVCAVLFAFIMISPWDKIVVFVIPMPAILYALLFLGYTIY
                     MGRRGGDNVNHDAHLWGAIYGVLFTIAVRPGIVGNFLNELSQPRF"
     gene            complement(1401..1874)
                     /locus_tag="VKU83_03050"
     CDS             complement(1401..1874)
                     /locus_tag="VKU83_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HLI92553.1"
                     /translation="MKIQLWSIGKEHEPYVKAGTEDFTRRVSRYYPVEWRLIAPPKQA
                     GSLAVPELKKKEGEIILAQLSKDDWLVALDERGKLLSSEGLALLLRQRANESVKNLVF
                     LIGGTYGLDETVLERSDLRWSLSTLTFPHQLVRLMLAEQLYRACSILRNEKYHHS"
     gene            complement(1901..3334)
                     /locus_tag="VKU83_03055"
     CDS             complement(1901..3334)
                     /locus_tag="VKU83_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI92554.1"
                     /translation="MRFLSQKLLPLEQRPRLFAGAIEWRRKLRSAGIAALRLLEQIKR
                     IGFTAEMDDYEKRKLGIFNQLNFLQLLTGIAIPLAGLVRNHHFPQMAWTMACIPALVS
                     MIVLACNARREYYLAQIAYFVGYPIATSFVYFWGFNMGVELSFILYGILSVFFIQDLS
                     QMLFALALSMASYFGLAILCKNYTYELSAASLYFYFFNQLLALGFIFYGLFLIKKENT
                     GYQYSILRQKEEIAQNEQLLKQQTEELKRLNAFKGRLFSIIAHDLKSPIYALRNLFRN
                     MQQYDLPAQDIKDMVPEVVNELTYTSSLMENLLVWARSQMRADSVKPQPIELNAVVDD
                     VSRLLKLQADAKQISLKVNGEVPVWGFADKDMVNLILRNLISNAIKYTPHGGEITVTA
                     ENKPHGVAFCVQDTGTGIDADSLEKINSNSFFTTKGTAGESGTGLGLMLCREFAVRNR
                     GELVVESEPGKGSRFTVLLPAPPAVNA"
     gene            3594..>4417
                     /locus_tag="VKU83_03060"
     CDS             3594..>4417
                     /locus_tag="VKU83_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI92555.1"
                     /translation="MKVSAATALGLTIVPRHVLGGKGFVAPSDKVTLAYIGTGTQGTR
                     ELLTMLANPHFQVIAVADPNKYAVGYKDWAEGWLLSEIRTAVKDPNWKSGGDNKIPGG
                     RDCGKSIVETYYSNNRPELKYKGCAAYADFRELLEKEKDLDAVKVMTPDHLHGVFATA
                     AIKRGKHILIHKPLSNRLMEGKKVVELARNSNVITHLIPWDANGSMTQVMEWINGGEI
                     GTLLEVHNWTNRPVWPQYATIPTDNPPVPDGFEWDLWLGPEATRPYSPEYTNMVFRG"
BASE COUNT         1073 a         1185 c         1206 g          953 t
ORIGIN      
        1 ataactatac ccgcaatttt tttcgccacc gcattctccc actcgtccgg gaggcgtatc
       61 cggcggcgct gcagaacctg gcggcgaaca tcgaccgctt ccgcggtatc gagctcctgt
      121 acacgcaggc tgtcgaactt cagaaaaaga aactcctcga ataccgcgga ggagaggttc
      181 atatccctgt cgggcggttg aaaaaggcga ctccgcttat cacgctcctc tacgagatct
      241 tttctcccta tggcttcacg ccgcaacagg cgccggcgat tggctcgctt ctggaaagcg
      301 acaccggcaa atatatctgt tccgctaccc atcgggtcct caggaacagg agctggctga
      361 tcctttcgcc cctggaatcg atgaaggcga caaccatcct cattgagtcc ggcgagagcg
      421 ccgtcgaatt cgcgcagggc tgcctgcgat tcaaacggac catcccggcc agccctcctc
      481 ccgcagatgc gatggtcgcc tggctggatg caaaagagat cattttcccc ctgatccttc
      541 gaccctggca gcccggtgat tatttttatc ctttggggat gcgcaagaaa aagaagcttg
      601 cccggttctt tatcgatcag aaactgtcgg cgaccgaaaa agaaaaaatc tgggtgctgg
      661 aaatgaacaa gaagatcatt tgggttgtcg gccggcgcat tgatcaccgt ttccggatta
      721 cgccgtcaac gaaggacgca tggcagatca gcttcagaag cgcggctggc tcaactcgtt
      781 gaggaaattg cctacgattc ctggccggac ggcgatcgta aaaagcaccc cgtatatcgc
      841 tccccaaagg tgcgcgtcat ggttgacgtt atcgccgccg cggcggccca tatagatcgt
      901 ataacccagg aagagcagcg cgtagaggat ggctggcatg gggatgacga acacgactat
      961 tttatcccac gggctgatca taatgaacgc gaacaggaca gcgcagaccg cgcccgacgc
     1021 gcccaggcta cggtagttgt aattatcgcg ctgcttgaaa tatgagggga tattcgctac
     1081 gatcagcgcc gtgatgtaga ggatggtgaa ataataatgc tggagcccca ggatgcccat
     1141 atagtaattt tcaagggccc gcccaaaaaa atacaatgtg aacatgttga aggcaaggtg
     1201 catgagatcg gcatggatga agccgcaggt gacgaaacgg tagtattgat gacgcaaatg
     1261 gaccgcgggg ggccagaaaa taagcgcatc cataagccgg gaattattga aggataggat
     1321 agagatgata gtggtaagga taatgatgat catcgtgatc gactgggaca tcggaatttt
     1381 ttttctaaag ataagcgaaa ttatgaatgg tggtatttct cgttgcgcag gatactgcac
     1441 gcgcgataga gctgctcagc cagcatcaac cgcaccagtt gatgcggaaa ggtgagcgta
     1501 gagagcgacc agcgaagatc ggaccgttcc aggaccgttt catccagtcc gtacgtgccg
     1561 ccgatcagga ataccaggtt cttaacgctt tcgtttgccc gctgccggag gagcagtgca
     1621 aggccttccg agctcaggag tttaccccgc tcatccaggg ccaccagcca atcgtccttt
     1681 gacagctgtg cgaggatgat ctcgccttct ttttttttca gttccggaac ggcaagcgag
     1741 cccgcctgct tcggcggtgc gatcaaccgc cattccaccg gataatagcg ggatacccgt
     1801 ctcgtgaagt cttccgtccc ggctttgaca taaggctcgt gttcctttcc gatggaccaa
     1861 agttgaattt tcatagccga aaactaggaa taaattacga ttacgcgttt accgccggag
     1921 gggcgggcaa gaggacggtg aaccggctgc ctttccccgg ctcactttca accaccagct
     1981 cgccgcgatt gcggacggcg aattcccggc aaagcatcaa gccgagtccc gtccctgatt
     2041 cgccggcggt ccccttcgtc gtaaagaacg aattgctgtt gatcttttcg agcgagtcgg
     2101 catcgatgcc cgtaccggtg tcctgcacgc agaaggctac gccgtgcggc ttattctctg
     2161 cagtgacggt gatctcgccg ccatgcggcg tatatttgat cgcgttggaa atcaggttac
     2221 gaagaatgag gtttaccata tccttatcgg caaatcccca aaccggtact tcgccattca
     2281 ccttcaggct gatctgcttc gcgtccgcct gcagcttcag gagccggctg acgtcgtcga
     2341 ccacggcgtt cagctcaatc ggctgcggtt tgacgctgtc ggcgcgcatc tggctacgcg
     2401 cccaaacgag cagattctcc atcaggctgg aagtataggt caactcgttg acgacttcgg
     2461 gcaccatatc tttgatgtcc tgtgcaggca ggtcgtactg ctgcatgttc cggaaaaggt
     2521 tccgcaaggc atagatcggc gacttcaggt catgcgcgat gatggaaaat agccggccct
     2581 tgaaagcgtt caatcgtttc agttcctccg tctgttgctt gagcagctgc tcgttctgcg
     2641 cgatctcttc cttttggcgc aggatactat actgataacc ggtattctct ttcttgatca
     2701 ggaacagccc atagaaaatg aaacccagtg cgagcaactg gttaaagaaa taaaaataaa
     2761 ggcttgcagc cgagagttcg taggtgtaat tcttgcagag gatcgccagc ccgaaatagc
     2821 tggccatcga cagtgcaagc gcgaagagca tctggctgag atcctggatg aagaaaacgg
     2881 agaggatgcc gtacaggatg aacgataact ccacgcccat attgaatccc cagaaataga
     2941 cgaaactggt ggcaatggga tagcctacaa aataggcgat ctgcgccagg taatattcac
     3001 gacgggcgtt gcacgcgagt acgatcatgc tgaccagcgc gggaatgcag gccatggtcc
     3061 acgccatttg cgggaaatgg tggttgcgaa ctaaccctgc aaggggaatg gcgataccgg
     3121 ttaaaagctg aagaaaattg agctggttga agatgccgag ctttcgcttt tcataatcgt
     3181 ccatctccgc agtgaaaccg atccttttga tctgttcaag caagcgaagg gctgcaattc
     3241 cggcggacct aagctttcgc cgccactcga tggcgcctgc gaacagccgc ggcctttgct
     3301 ccaagggcag gagtttctgc gataggaatc tcattgggtt tcaagtacgt gataaacagg
     3361 atccgtaaaa tagagattat tcttcactta gtcaagcccc cggtaaaaaa cgtccccggc
     3421 cttacgcgcc gcggctaatt gtaccggggc ttctaaatat ttcgaggaac aatttcccca
     3481 atgcggcgca atccgctaaa aaagatcaga atcccttatc tttaggcagc taacgataaa
     3541 atctgccaat catgagatcc gctaaccgcc gcacaatcga ccgtcgtaaa ttcatgaaag
     3601 tttctgccgc cacagccctc gggctcacta tcgtgcccag gcacgtgctg ggcggaaagg
     3661 gctttgtcgc gcccagcgac aaggtcaccc tcgcctacat tggaacaggc acccagggca
     3721 cccgtgaact gctcaccatg cttgccaacc cgcatttcca ggtaatcgcc gtggccgacc
     3781 cgaacaagta tgcggtcggt tacaaggatt gggccgaagg gtggcttctc agcgagattc
     3841 gtacagcggt gaaggatccc aactggaaat ccggtggtga caacaagatt ccgggtggcc
     3901 gcgactgcgg caagtctatc gtagaaactt attattcgaa caacaggccg gaactgaaat
     3961 ataagggctg cgcggcttac gccgattttc gcgagttgct ggaaaaggaa aaagacctgg
     4021 acgctgttaa ggtgatgacg ccggatcatc tccatggcgt attcgccacc gcggcaatca
     4081 aaagaggcaa gcatatcctc attcacaagc cgctttccaa ccggttgatg gaagggaaga
     4141 aggtcgtcga gctggcgcgg aattccaatg tcatcacgca cctgatcccc tgggacgcca
     4201 acggatcgat gacgcaggtc atggaatgga tcaatggtgg cgagatcggc accttgctgg
     4261 aagtgcataa ctggaccaac aggcccgtat ggccgcaata tgcgacgata ccgaccgaca
     4321 acccgccggt accggacggt tttgagtggg acctctggct cggccctgag gcgacccggc
     4381 cctattctcc ggagtatacg aatatggtat tccgcgg
//
LOCUS       DATGKE010000177        10110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_270167, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000177 DATGKE010000000
VERSION     DATGKE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270167"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VKU83_03065"
     CDS             complement(<1..285)
                     /locus_tag="VKU83_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92556.1"
                     /translation="MIVLYIIAGIIVLFLVVAAFVGRGWSFEKNILIQASRSNVWSHV
                     NSLGAINTWNPWLELDPAVSQSISGVDGTPGATYSWDSKVKNVGAGRQTIV"
     gene            375..1154
                     /locus_tag="VKU83_03070"
     CDS             375..1154
                     /locus_tag="VKU83_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HLI92557.1"
                     /translation="MKALTKGLSFGALALAVAGALAFICYDRAAFPQVAVGAPGFAVV
                     ELFTSEGCSSCPPADQLVARLQEEDRKEPVYILAFHVDYWDRLGWKDVFSSPRYTQRQ
                     NQYARWLSLNSIYTPQVVVNGHQEMVGSQESAVRKAIDDGLDQTPAEDLTFVAVREEQ
                     GKVGWHYQVSHPERDQVLVVALIQKKATTNVRGGENNGRTLSHVQIVRGLASQSMGRG
                     GAGSGDLTLPAGVRADEEELIAFVQDGVTGKILAAARAAIP"
     gene            1311..2153
                     /locus_tag="VKU83_03075"
     CDS             1311..2153
                     /locus_tag="VKU83_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI92558.1"
                     /translation="MRPALPLLLLALGSCACHQPQPAAPVVYGDNPAAGHYLSTRGFK
                     MYYETYGEGAPLLMLHINGGSIANFANQIPYFAAHYRVIAVDTRAHGKSVDAGDSLTF
                     EMMADDFNALLDSLRLDSCYVIGWSDGGISGLQLAIRHPDKVKKLVVSGPNLWPDTTG
                     LVPFIFHYLQRVADTLRRQPQTPAIKNQLKIVELDLKEPHMTLDQLAKIRCPTLVMGG
                     DHDGIPSYHLWLISQHIPMSYLWIVPNSGHSVAIYKKDQFNPVVEDFLHEPYRKIEGL
                     DVLH"
     gene            complement(2253..3287)
                     /locus_tag="VKU83_03080"
     CDS             complement(2253..3287)
                     /locus_tag="VKU83_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92559.1"
                     /translation="MKNQWWGAGAGEWKTVPEKTLMDRASTLFWLADGGGRLIYFNNA
                     FYRQFGVEESPADTKAQEFVPRDMAVFFAEKHRRVAQSGIPYRRVHHFTRTDGRCCSY
                     LISIYTIDSPGEPLTGGEAWDVSRFSEEKTEQEEKFARKIVEVREEERTYLGHELHDN
                     VIQIMISARLHLDGLKPKTDDERAMKSRATSLILSAIEESRRLSRQLAASQLKNSGLL
                     TAIAQLLNDLRSTGRFNIKFNSSLDIDTEYHLSEEKKVALYRIVQEELSNIIRHSRAR
                     NIRVNIMQNNSGVILYTFDDGIGFDLKQAPKGMGLASIRNRAQVLNGIVDIATSPGKG
                     CSVTVLMPYD"
     gene            complement(3416..3778)
                     /locus_tag="VKU83_03085"
     CDS             complement(3416..3778)
                     /locus_tag="VKU83_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92560.1"
                     /translation="MTKILLADDHSAIRQRLRQILLEGFPSVQIAEVSDGDLLVREAL
                     EGGWDLIVADISMPGQSGIEATRRIRARFPRLPVLLLSIYYDEDYARHVVSAGASAYL
                     CKENAQDELVETISRLIV"
     gene            complement(3903..4529)
                     /locus_tag="VKU83_03090"
     CDS             complement(3903..4529)
                     /locus_tag="VKU83_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92561.1"
                     /translation="MRILIADDHAVVRRGLSEILLEAYPAATIEQAGDADTLLQRSTE
                     EAWDIVISDLMMPGRSVLEVLQQLRQVHPQLPVLVLSIFPEEQYATRVFKAGAAGYIN
                     KDAAPTELVKAVERILQGRKYITARIAEKLAGDLSLDKDKAPHELLSDREFHVMKLLA
                     TGKTITEIAGELSLSTTTISTYRSRIMGKMKMKANAELARYALENGLI"
     gene            complement(4545..6209)
                     /locus_tag="VKU83_03095"
     CDS             complement(4545..6209)
                     /locus_tag="VKU83_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HLI92562.1"
                     /translation="MLRNLKTALLGAFGKRNIRRTRLEKSRRLYATVSQVNKTIIYAR
                     EPIDLFRRVCSAVVETGGFRMAWVGIIDPETNTVVPVCHVGHEAGYLSIRRFSIDARP
                     DGTGMGPTGRAVRERHHYICHNIATDPAMRPWQADALERGYRSSMALPILKFGDSSGV
                     FCLYSEEADFFDEEEVRMLDEVARDISYTLEVIEQEELRKKAETALRAEKQFSDSIIN
                     MLPGVFYMYDERLSFLRWNSRFEEVTGYDHEQFATLHVLDLFNAGQRELIRKKTESVF
                     VTGRCDVEAELTTRTGENVPFYFSGIRISYQDKPAVLGIGFDITRLKETENLLKQSEE
                     DLRDLASNLQNVREEERAAIAREIHDELGQQLTAIKLDVSWLDRKIAGDEMIKERIAG
                     VLGMLTGMIHSIRRISTELRPSILDDLGLVEALKWHVRDFQKRTGIRVILDCTTEPLK
                     LEPAVNTGLFRIFQETLTNIARHSEASVVSARLFPDNGQLVLSIADNGKGFDPGMAKK
                     KKTLGLLGMKERTLMMNGSYEIRSRPGEGTSLRFSVPLDSLEQPSA"
     gene            6294..7604
                     /locus_tag="VKU83_03100"
     CDS             6294..7604
                     /locus_tag="VKU83_03100"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLI92563.1"
                     /translation="MKAFGPFCMKVISGFFLITIYCALSLSPLNAAGQTPADFQRISG
                     LFLKYYNANNADSLFALFSPEVKKALPSDKTGELLGSLHAQLGLWKTLGLTRQSRHYA
                     LYKAGFEHSLITLSLALDSNEQINGFHFLPYKEDSSAGAHDGETLSLKTATGTLVGTL
                     VVPADQKKVPVVLIIAGSGPTDRDGNSPNMGLHSDMYKLLADSLASHGIASFRYDKRG
                     VGASAGAMTSKADLTFDDYIADAAGWIELLRKDPRFSRTVVLGHSEGSLVGMVAAREA
                     KADAYISAAGAGDRIDKVLLPQIEVQSAGLADSARRLFDSLAEGLAVHVASGPLLVLF
                     RPSIQRYMISWMKYDPRNEIKKLTVPILILQGTADLQVTIADAEKLKAARPDAILNLI
                     PGMNHIFRDAGAGADRAANLATYSNPSLPLDATLVGDIVSFINR"
     gene            complement(7660..8874)
                     /locus_tag="VKU83_03105"
     CDS             complement(7660..8874)
                     /locus_tag="VKU83_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92564.1"
                     /translation="MKLTFFLVAFFLILMRVCLPAIGLWHHGPEQGTMVMESDNYHEG
                     IWWSGKYRLSDDDRSVSSISPGGYLKFRENDTTMKLESDLQGRISYTLYNGREHLPLN
                     DSGRNFIAAQLQKMIRLGFFGDQRAERIYRQGGVSALLGELSRIKMEGGREPYLKLLL
                     RTDSLTADQRIALLRLTDSSQDMGERQHLLEEFHRARLDDPAVAREWLTVVGHLDASY
                     MKKNLLLKYIDSSLTVDQFDTVLAITSRFESDADEQDIYKSLVDLPQLRMDDSAYAQP
                     WLSAVGQLEASYQKKDLLLTFIGRDSAGAGLAPRLFDTVVAITGRFGSSADELEVYKR
                     LSRLPGITAEEWADLLQASVTLDADYLKGELLRTIALKMPRTDSLRAGYRSAAKSIQG
                     DDEYGKTMRAIE"
     gene            complement(8879..9313)
                     /locus_tag="VKU83_03110"
     CDS             complement(8879..9313)
                     /locus_tag="VKU83_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92565.1"
                     /translation="MKKTFLIMTLLGGSLATYAQDKPEPLVDRSLISDILSTTAIVFV
                     VYLLADFIVRIIRHNFDYRLKSKIVDKQTPEGVITQLVQPEQTSTRRAVLQWFCTLVG
                     VGIGFALMAATEPVGLHSLAIMAFSVAAGLGVYYFLGKKPKD"
     gene            complement(9318..9773)
                     /locus_tag="VKU83_03115"
     CDS             complement(9318..9773)
                     /locus_tag="VKU83_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92566.1"
                     /translation="MSGKHLTEDMLQQCALNPGECPDETLTHLFECAECRKVVEAYEH
                     LGTLLRDQPAPVFEFDLAAAVIARLDSPELAVESASGARQTGRFLIPLIAVLVAFPAW
                     FFWKTAYFVFSAASTGLAGIILGFAGFTILSAFYKYYRKYQQVLHLLNS"
     gene            complement(9770..>10110)
                     /locus_tag="VKU83_03120"
     CDS             complement(9770..>10110)
                     /locus_tag="VKU83_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HLI92567.1"
                     /translation="LNRLEKKQLDYFLAAERDDDGKGEPSTLVFRAPEETDQRLLRKE
                     LRELIAIEIGRLPPLFQTLIGLYHQEDLSYQEIGGITGLPEGTIKSYLFRARKMLKET
                     TLSSYKKETL"
BASE COUNT         2121 a         2849 c         2744 g         2396 t
ORIGIN      
        1 gacgatcgtt tgcctgcccg cgccgacatt cttcacctta ctgtcccagc tatacgtagc
       61 tccgggcgta ccgtcgacgc cgctgatcga ttggctgacc gccgggtcca gttccagcca
      121 cgggttccag gtatttatcg cgccgaggga attgacatgg ctccagacat tagatcggga
      181 cgcctgaata agaatgttct tttcaaagct ccatcctctt cccacgaatg cagccactac
      241 cagaaagagc acgataatac ctgcaatgat gtaaaggact atcataagaa tgctattttg
      301 gtttgtataa atataaatca ttttgggttt catttgggcg aatttctgcg atctttgctt
      361 ctatacgatt catcatgaaa gctttgacga agggcctgtc gtttggcgcc ctggcacttg
      421 cggtcgccgg cgcgctggcc tttatctgtt acgaccgggc cgcatttccg caggtcgcgg
      481 tgggcgcgcc cggattcgct gttgtcgagt tatttacatc cgaaggatgt tcgagctgcc
      541 ctccggcgga tcagctggtc gctcgtctgc aggaggaaga caggaaggag ccggtctata
      601 ttctcgcctt tcatgtcgac tactgggacc ggctgggttg gaaagatgtt ttcagcagcc
      661 cccgatatac ccaacgtcaa aatcagtacg ccaggtggtt aagcctgaat tccatctata
      721 cgccgcaggt ggtggtcaat ggtcatcagg aaatggttgg ctcccaggaa tcggcagtgc
      781 gaaaagccat cgatgacggc ttggaccaaa cgccggcgga agatttgacc ttcgtggcgg
      841 ttcgggagga acagggcaaa gtgggctggc attaccaggt cagtcatccg gaaagggacc
      901 aggtgctggt tgtagccctc atccagaaga aagcaaccac caatgtccgg ggcggcgaga
      961 ataatggccg gaccctttct catgtgcaga tcgtccgggg cctggccagc caaagtatgg
     1021 gccggggagg ggcgggatcc ggcgacctga cccttcccgc cggtgtccgt gcggacgagg
     1081 aagagctgat tgccttcgtc caggatggcg ttaccggaaa gatcctggca gcggccaggg
     1141 cggccatccc ctaggcatgg tttccaacgc ccgccccggg ggtctggtat ccttttccct
     1201 taattgggcc gaaatcggcc caattaacct gacacctatc ccgcgccctg ttgtgccggg
     1261 tgatttgtcc cgggattttg ggttatattt aaaacctcaa cattctgttt atgagaccag
     1321 cactgccgct cctgctgctc gccctgggct cctgcgcctg ccaccagccc cagcctgccg
     1381 cgccggtcgt ttacggcgat aaccctgcag ccggccacta ccttagcacc aggggcttca
     1441 aaatgtacta tgaaacttac ggtgaaggcg ctcctctcct catgctccac atcaacggcg
     1501 gatccatcgc caattttgcc aaccaaattc catatttcgc tgcccattac cgggtgatcg
     1561 ccgtcgacac gcgcgcccat ggcaagtccg tggacgcggg agattcactg acattcgaaa
     1621 tgatggccga tgacttcaac gccctgctcg atagcctgcg cctagacagt tgctatgtta
     1681 tcggctggag cgacggtggc atcagcggct tgcagctggc catccggcac cctgataagg
     1741 tgaagaagct cgtggtcagc gggccgaacc tctggccgga caccacaggc cttgtgccct
     1801 tcatcttcca ttatttgcaa cgtgtcgcag atacgctgcg ccgtcagccg cagacgccgg
     1861 caataaagaa ccagctgaag atcgtcgagc tggacctcaa agagcctcat atgacgctgg
     1921 atcagttggc gaagatccgt tgtccgacgc tcgtgatggg cggggaccac gacggcattc
     1981 cctcctatca tctctggctg atatcacagc atatcccaat gtcctatctc tggatcgttc
     2041 ccaattcagg gcattcggtg gccatttata aaaaggatca attcaacccg gtcgtcgaag
     2101 actttttaca cgagccttac cggaagatag aagggctcga cgtgctccat tgaggattgg
     2161 ggaaaaccaa atcaccctac acctccgcct gaccacgctc acagccagga caggagggta
     2221 gagttttcag aggtatgaac cgtcgttaat ggtcaatcat aaggcatcaa taccgttacc
     2281 gaacagccct tgccgggcga ggttgcgatg tcgacgattc catttagcac ctgggcccgg
     2341 ttgcggatgc ttgccagacc catgcctttt ggcgcctgtt tcagatcgaa gccgatgccg
     2401 tcgtcaaaag tgtacaggat gacgccggag ttgttttgca tgatgttgac ccggatgttc
     2461 cgcgccctgc tgtggcggat aatattgctg agctcctcct gtacaatgcg gtacaaagcc
     2521 accttttttt cctccgagag gtgatattcg gtatcaatat ccagcgatga attgaatttg
     2581 atgttgaacc ggccggtgct ccgcagatcg ttcaaaagct gcgcaatggc ggtgaggagc
     2641 cctgaatttt ttagctggga tgcggccagt tgtcgcgaaa ggcggcggct ttcctcgatg
     2701 gccgacagga taagggaggt agccctggat ttcatcgcac gctcgtcgtc cgtcttaggt
     2761 ttaaggccgt caagatgaag acgggcggaa atcatgatct ggataacatt gtcgtgcaac
     2821 tcatgtccga ggtaggtgcg ttcctcttcc cggacttcca caatcttcct ggcgaatttt
     2881 tcttcctgct ccgttttctc ttccgaaaac ctgctgacat cccacgcttc gccgccggta
     2941 aggggctccc cgggggagtc gatggtataa atgctgatca ggtaactgca gcatcgcccg
     3001 tccgtccgcg taaaatggtg caccctgcgg taggggatac ccgactgagc gacccgccgg
     3061 tgcttttcgg caaaaaaaac ggccatatcc cgcgggacaa attcctgcgc ctttgtatcg
     3121 gccgggcttt cctccactcc aaactgccgg taaaatgcgt tgttgaaata gatgagcctg
     3181 cctccgccat cggccagcca gaaaagggtg ctggcccgat ccatcaaggt cttttccggc
     3241 acggttttcc actcgccggc tcctgcaccc caccattggt ttttcatatt gtccctctca
     3301 gtcatctctg tttccatggc cgtttgaggg tgcaaaataa gttcgttatg cctgactgca
     3361 gaatagtact tggtatctat tcggggtaca tttttataaa gccgggttgt agattttata
     3421 ctataaggcg gctgatcgtt tcaacgagtt catcctgagc attttccttg catagatacg
     3481 cggatgcgcc ggcgctgacc acatgacgcg catagtcttc gtcgtaataa atactcagca
     3541 ggagcacagg gagacgcgga aaacgggcgc ggatacgccg cgtggcttca atgccgctct
     3601 ggccgggcat agaaatgtcc gcgacgataa ggtcccagcc gccttcgagc gcttcgcgaa
     3661 ccagcagatc gccgtcggag acctcggcga tttgaaccga aggaaagccc tcgagcagga
     3721 tttgcctcag gcgctggcgg attgcggagt gatcgtctgc caataatatc ttcgtcatgc
     3781 agggccggat tatcctctgc cggcgtttgt gcctggcagc agggcattgt aaaaatatgc
     3841 tgcttaccga ctgcgaggta taaatgaatg atgaagactt ttgtagattt tttgctacac
     3901 ggtcagatca ggccgttttc gagggcgtat cgcgccaatt cggcattggc cttcattttc
     3961 attttcccca tgatccggct gcgataggtg cttatcgttg tggtactaag ggaaagctcg
     4021 ccggcgatct ccgtaattgt cttacccgtc gccagcagtt tcattacgtg gaactcgcgg
     4081 tccgaaagaa gttcgtgcgg cgctttgtcc ttatcgaggc tgaggtcacc ggccagcttt
     4141 tcggcgatcc tggcggtgat gtacttccgg ccctgcagga tcctttcgac cgctttaaca
     4201 agttccgttg gagcggcatc cttgttgata tacccggccg cgcctgcctt gaagacccgg
     4261 gtcgcgtatt gttcttcggg aaagatgctg aggacgagca caggcagctg tgggtgaacc
     4321 tgcctgagct gctgcaagac ttcgagaacg ctgcggccgg gcatcatcag gtcactgata
     4381 acgatatccc aggcttcttc cgtcgaccgc tgcaacaacg tatcggcatc accggcctgc
     4441 tcgatggtgg cggcagggta ggcttccagc aggatctccg aaaggccgcg gcggacgacg
     4501 gcgtgatcgt cggctataag gatgcgcata tgaggttgat tggattaggc agagggttgc
     4561 tccaggctgt ccagcggaac gctgaaacgg agactggttc cctcgcccgg cctgctcctg
     4621 atctcatagg acccgttcat catcagggtt cgctctttca tcccgagcaa tcccaaggtc
     4681 tttttctttt tggccatgcc cggatcgaac ccctttccat tgtccgcgat tgacaacacc
     4741 aactggccat tgtcgggaaa aagccgtgcc gacacgacgg aagcctccga gtgacgggct
     4801 atattggtca gggtttcctg gaagatgcga aataatccgg tgttgaccgc cggctccagc
     4861 ttaagcggct ccgtggtgca gtccaatatg acgcgaatgc ctgtccgctt ttggaaatcg
     4921 cgcacgtgcc atttcaacgc ctccacgagc cccagatcgt cgaggatgct gggccgtaat
     4981 tcggtggaga tccggcggat cgaatggatc atcccggtca gcatcccgag cacgcccgcg
     5041 atccgctcct tgatcatctc atctccggct atcttccggt cgagccatga gacatccagc
     5101 ttgatcgccg tcagctgctg gcctaactcg tcgtggattt cgcgggcgat ggcggcgcgt
     5161 tcctcttccc ggacgttttg caggttggag gccaggtcgc gcaaatcctc ctcgctctgt
     5221 ttgaggaggt tttcagtctc tttcaggcgc gtgatatcga atcctatgcc cagcacagcc
     5281 ggcttgtcct ggtaacttat cctgattcct gagaaataga acggtacatt ttcgccggtc
     5341 cgtgtcgtca gttcggcttc tacatcgcaa cggccggtta caaaaacact ttcggttttt
     5401 tttctgatca gctcacgctg gccggcattg aacaggtcca ggacatgaag ggtggcaaat
     5461 tgttcgtgat cgtatccggt gacctcttca aatctgctgt tccagcggag gaaggacagc
     5521 cgctcgtcgt acatataaaa tacgccgggc aacatattga tgatcgagtc ggagaattgc
     5581 ttttccgcac gcaatgccgt ttcggctttt tttcgcaact cctcctgctc gatgacctcc
     5641 agggtgtagg aaatatcccg ggccacctca tccagcatcc ggacctcttc ttcgtcaaaa
     5701 aaatctgctt cttccgagta caggcaaaaa acgccggatg aatcgccgaa cttcaagatg
     5761 ggcaaggcca tcgatgaccg gtagcccctt tccagcgcgt ccgcctgcca gggccgcatc
     5821 gccggatctg tggcgatatt gtggcagata tagtgatgcc gctcacgaac agcccttccc
     5881 gttggcccca ttcccgtacc gtccgggcgg gcgtcgatgc tgaaccggcg gatgcttagg
     5941 tagccggctt catgccctac atgacaaaca ggcacgaccg tgttcgtttc gggatcgata
     6001 atccctaccc atgccatccg gaagccgccg gtttcgacga cagccgaaca gacccggcgg
     6061 aacaggtcga tcggctcgcg ggcataaatg atcgttttat tgacctggct aaccgtagcg
     6121 tataagcgtc tcgatttctc cagcctggtt cgccggatat tgcgcttccc aaatgcccct
     6181 aataacgccg ttttaaggtt cctgagcaat tttcagcagt tgtgtattat aaggtacaat
     6241 cttaattcag ctttccggct ttttttgttt caaattattt atttaccttg aaaatgaagg
     6301 cctttggccc gttttgcatg aaagtgatct ccgggttttt tttaataact atctactgcg
     6361 ccctttcgct gtcgccgctg aacgctgcag gtcagacgcc ggcggatttt cagcggatct
     6421 ccggactatt tctcaagtat tataatgcca acaatgcgga cagtcttttt gccctgtttt
     6481 ccccggaagt gaaaaaagcg ctgccatccg acaagaccgg agagctgctt ggctcgcttc
     6541 atgcccagct gggtctttgg aaaacgcttg gcctgacccg gcaatcaagg cactatgcgc
     6601 tctacaaggc gggattcgaa cattcgctca taacgctcag cctcgccctc gacagcaatg
     6661 agcagatcaa cggctttcat tttcttccgt acaaggagga ttcttcggct ggcgcccatg
     6721 atggtgagac gctgtccctc aaaactgcca ccggcacgct tgtcggcacc cttgtggtcc
     6781 cggcggatca aaagaaggta cctgtcgtcc tgatcatcgc cggttcagga ccaaccgatc
     6841 gtgacgggaa ttcaccgaac atgggcctcc attccgatat gtataaattg ctggccgact
     6901 ccctggccag ccacggcata gccagtttca ggtacgacaa gcggggtgta ggcgccagcg
     6961 ccggcgccat gacctccaaa gctgacctga cctttgatga ctatattgcc gacgcggcag
     7021 gatggattga acttttgcgg aaagacccga ggttttcccg caccgttgtt cttggccata
     7081 gcgaagggtc gctcgtaggg atggtggcgg cccgcgaggc gaaagccgac gcgtatattt
     7141 cggcggccgg cgcgggtgac cgtattgaca aggtcttgct gccacagatc gaagtgcagt
     7201 cggccggcct cgctgacagc gccaggagat tgtttgacag ccttgccgaa ggcctcgccg
     7261 tgcatgttgc aagcgggccg ttgcttgtcc tgttcaggcc atccattcaa cgctatatga
     7321 tttcgtggat gaagtacgat ccgcggaatg agatcaagaa actgaccgtt cccatactga
     7381 ttttgcaggg gacggccgac ctgcaggtta cgatcgccga tgcggagaag ctcaaggccg
     7441 ccaggccgga cgccatcctg aacctgattc cgggaatgaa ccatatcttc cgggatgccg
     7501 gcgccggcgc ggaccgcgcc gccaatctcg ccacctatag caatccgtcc cttccactgg
     7561 atgccacgct ggtgggggat atcgtctcgt ttatcaaccg ttaagagggt tggtcgtgcc
     7621 cctgcatcat ggggtgtcag tcaacgcttt atccctgttt cattcgatag cccgcatggt
     7681 ctttccgtat tcgtcgtctc cctggatgct tttcgctgcc gatctatatc cggcgcgcaa
     7741 actgtccgtc cggggcattt tcagcgcgat ggtccgcagg agttcacctt tgagatagtc
     7801 cgcgtcgagt gttaccgatg cctgcaaaag atcagcccat tcttccgcgg tgattccggg
     7861 aagtcggctg agtcgtttat agacttccag ttcgtcggca ctcgacccga aacgacccgt
     7921 gatcgcaacg accgtatcaa aaagccgtgg cgccaggcct gcgcctgcag aatccctccc
     7981 gataaaggtc agcaacaaat cctttttctg atacgaggcc tcaagctgcc caaccgcgct
     8041 caaccatggt tgggcgtagg cagaatcatc catccgcagc tgcggcagat ccacaagtga
     8101 tttgtaaata tcctgctcat cggcgtcgga ttcaaaccgg gaagtgattg ccagaaccgt
     8161 atcaaactgg tcaacggtca ggctgctgtc gatgtacttt agcagcaggt tttttttcat
     8221 gtaggaggcg tcgaggtggc cgacaacggt caaccattcc cttgcgacag cgggatcgtc
     8281 cagccgggcg cggtgaaatt cctccagcaa atgctggcgc tcacccatat cctggctgct
     8341 gtcggttaac cgcaatagcg cgattcgttg gtcagccgtc aaactgtcgg tacgtagcag
     8401 cagcttgagg tagggctcgc ggccgccttc catttttatc ctggaaagtt cgccgagcag
     8461 cgcggaaaca ccgccctgcc ggtatatccg ttcagcccgc tggtcgccaa agaagcccag
     8521 ccggatcatc ttttgtaact gggctgcaat gaaatttcga ccgctgtcat tcaatggaag
     8581 gtgctcgcgc ccgttataca aggtatagct gatcctcccc tgcaaatcgc tttccagctt
     8641 catggtggta tcgttctctc tgaatttcag gtaaccaccc ggactgatgc ttgaaaccga
     8701 ccggtcgtca tcgctcagcc ggtacttgcc cgaccaccag atcccttcat ggtagttgtc
     8761 gctctccatg accattgtgc cttgttccgg cccatgatgc cacaggccaa tagccggcag
     8821 gcaaacgcgc atcaggatca gaaaaaaggc gacgagaaaa aaagtaagtt tcatacgatc
     8881 aatcttttgg ctttttgccg aggaaataat acactccaag cccggccgct acgctgaaag
     8941 ccatgatagc gagcgaatga agtcccaccg gctcggtggc ggccatcagg gcgaagccaa
     9001 taccgacgcc aaccagggtg cagaaccatt gcaggacggc gcgcctggta ctggtctgct
     9061 ccggttggac aagttgggtt ataacgcctt cgggcgtttg tttgtcaaca atcttgcttt
     9121 tcaggcggta gtcgaaattg tgccggatga tccgcacgat gaaatccgca agcaggtaga
     9181 cgacaaacac aattgcggtc gtactcaaga tatcggagat taatgaccgg tcgaccagcg
     9241 gttcaggttt gtcttgagcg taggtggcca ggctgccgcc caaaagcgtc ataatgagaa
     9301 aggttttttt catttgttta actgtttaaa agatgaagga cttgttgata tttgcgataa
     9361 tatttataaa aggcggacag tatcgtgaac cccgcaaatc ccaaaatgat ccccgcgagc
     9421 ccggtcgatg cggccgagaa tacaaaatag gcggtcttcc agaagaacca tgcgggaaag
     9481 gcaaccagca cggcgatcag ggggatcaaa aaccggccgg tttgccgcgc accggatgca
     9541 gattccaccg ccagctccgg ggaatcgagc cgagcgatca ccgcggccgc cagatcgaat
     9601 tcgaagaccg gtgcaggctg gtccctaagc agcgtgccca ggtgctcata cgcttccaca
     9661 accttgcggc attctgcgca ttcaaaaaga tgtgtcagcg tctcgtccgg gcattctccg
     9721 gggttcagcg cgcattgttg cagcatatct tcggtcaggt gctttccgct cataacgttt
     9781 cttttttata ggaggacaag gtggtttctt ttaacatctt tcgcgcccgg aacaagtagc
     9841 tcttgatcgt tccctccggc agtccggtga tcccgccgat ctcctggtag ctcaggtcct
     9901 cctggtggta aaggccgatc agcgtctgga acagcggcgg cagccggccg atctcgatcg
     9961 cgatcagctc gcgtagctct tttcgcagca agcgctgatc ggtttcctca ggcgcccgga
    10021 acaccagcgt gctgggttcg cctttcccgt cgtcatcgcg ttcagcggcc aggaagtagt
    10081 ccaattgttt tttttccagc cggttcaaac
//
LOCUS       DATGKE010000178         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1306816, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000178 DATGKE010000000
VERSION     DATGKE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306816"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..561)
                     /locus_tag="VKU83_03125"
     CDS             complement(106..561)
                     /locus_tag="VKU83_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HLI92568.1"
                     /translation="MQDDAISKFFGKYVDDDRFSVVSISPRMFRLLAKVDWDTVSPDL
                     KRTVSRLTSFRILSTSTSPMQFYKEALQKIDRRQYEELITVRDKTDDVHFLVKSTGNA
                     VQELLMIAGSPDSFTLVSFVGDIDLKSLSRLSADMGIKGMEELKDAKRP"
     gene            complement(649..1233)
                     /locus_tag="VKU83_03130"
     CDS             complement(649..1233)
                     /locus_tag="VKU83_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92569.1"
                     /translation="MEPVEIRGLLEKYWLAETTVAEEEQLAAWFRQQQEIPADLEPLR
                     ELFTWRVEESQVVAGEDLDRRILQRISRMESGGTVIPFRMAAAAAVVVSLAMGLLIAV
                     TAPRSNSTLPGAARRDAAPGAALVQVEKDNGQKTGGQDATLRVKDTYTDPRQALAAVR
                     NALLLASVRINEGAQITQKNITRLHNSWEVATGD"
     gene            complement(1221..1694)
                     /locus_tag="VKU83_03135"
     CDS             complement(1221..1694)
                     /locus_tag="VKU83_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI92570.1"
                     /translation="MKDKLFRFALRMLSNVQEAEDAIQEVMAGLWVRRDDWGNWKNLE
                     AYCMTATRNHCIDRIRRRRHRTLQENEIPDVAAGQDPYEKMMTKEMSYRIRQCMAELP
                     ENQQLVMHLREVEGFSYNEIAEVLQITLDQVKTNLFRGRNAIKNTIIKEEGIWSR"
     gene            complement(1871..>2216)
                     /locus_tag="VKU83_03140"
     CDS             complement(1871..>2216)
                     /locus_tag="VKU83_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HLI92571.1"
                     /translation="KDVKDYAAMMIKDHTAAADKLQAIAGKKNITLPTTLTPDAQKKI
                     DDLQKESGKKFDKDYISMMVSDHKKVISSFEDESKNGSDADIRGFADSTLHTLRVHLK
                     KAEQCEKMEKKM"
BASE COUNT          437 a          646 c          553 g          580 t
ORIGIN      
        1 tgtcaaattt gtgataaaac cggtcgagat gactttcctg cgcgttgcag gaggctgccg
       61 tcataaggat cagcagcgga gttagtaatt ttttcatatt gatgattatg gtcgttttgc
      121 atctttaagt tcctccattc ccttgatccc catatccgcg gatagccggg acaaggattt
      181 cagatcgata tccccaacga agctcaccag ggtgaaactg tccggacttc cggcgatcat
      241 gagcagttcc tgcaccgcgt tcccggtgct cttgacaaga aaatgcacgt catcggtctt
      301 atcgcggacc gtgatcagtt cctcatattg cctccggtcg atcttctgca gcgcctcctt
      361 gtagaattgc atggggctgg tggaagtact tagaatgcga aagctggtga gccggctgac
      421 cgttcgtttg agatcggggg aaacggtatc ccagtcgacc ttggcaagca gccggaacat
      481 ccttgggctt atcgagacaa ccgagaacct gtcgtcgtcc acgtatttgc cgaagaattt
      541 gctgatggca tcgtcctgca aagcctgccc tgcccagaag gagcagcgac agcaacaatg
      601 cccagggacg gaatttggct atactcatat tctttttttt atctgtggtc agtcgccggt
      661 agcgacttcc cagctattat gcagccgggt gatatttttt tgcgtaatct gcgcgccttc
      721 attgatcctg acggacgcca gcaacagggc attccgtacc gccgccagtg cctgccgcgg
      781 atccgtgtag gtgtctttga cccgcagggt ggcgtcttgc cccccggttt tttgcccgtt
      841 gtccttttcc acctgcacaa gcgcagcccc gggcgccgca tcgcggcgcg ccgcaccggg
      901 cagcgtcgaa ttactacggg gcgccgtaac cgcgatcagc aggcccatcg ccaaactgac
      961 gacaaccgcg gcggccgcgg ccatccggaa cggaataacg gtcccgccgg attccatcct
     1021 ggagatccgc tgcaaaatcc gccggtcaag atcctctcca gccacgacct gcgattcctc
     1081 cacccgccat gtgaataatt cccgcagcgg ctccaggtcg gcagggatct cctgttgctg
     1141 gcggaaccag gcggcgagtt gctcctcttc ggcgacggtc gtctccgcca gccagtactt
     1201 ctcaagcaat ccccttattt ctaccggctc catataccct cctctttgat gatagtgttc
     1261 ttgatggcgt tccgcccgcg gaacaaattt gttttcacct ggtccagcgt gatctgcaag
     1321 acttcggcga tttcgttata gctgaatccc tccacctccc gcaaatgcat caccagttgc
     1381 tggttttcgg gcaattccgc catgcattgt ctgattcgat aggacatttc cttggtcatc
     1441 attttttcgt atggatcctg gcccgcggcc acatccggga tctcgttttc ctgcaaggtc
     1501 cggtggcggc gccgccggat ccggtcgatg caatggtttc gggttgccgt catacaataa
     1561 gcctcgaggt tcttccagtt cccccagtcg tcccgccgca cccaaagccc cgccattacc
     1621 tcctggatcg catcctccgc ctcctgcacg ttggacaaca tgcgcaaggc aaaccggaac
     1681 agtttgtctt tcatgggcaa tatgcgctcc ttaaagactt cgacagacat gcttaatacc
     1741 atgacggaag gaaatggccg atgttacagc acaaaataaa aaaaacgggc agaaatttct
     1801 tcctgcccgc tctgaactgg gtctgcgccc aattacattt caatgggtaa ggcacttggc
     1861 attcgatcag ttacatcttt ttctccattt tttcacattg ttcggccttc ttcaggtgta
     1921 cccgcagcgt atggagcgtg ctgtcggcaa aaccccggat gtccgcgtca gagccattct
     1981 tcgattcgtc ttcaaacgag ctgattactt tcttgtgatc cgacaccatc atcgagatat
     2041 agtccttgtc gaatttcttg ccgctttcct tttgcaggtc gtcgatcttt ttctgggcat
     2101 cgggtgtcag tgtcgtaggt aacgtgatat tctttttgcc ggcaatggcc tgtaatttgt
     2161 ctgcagccgc ggtgtgatcc ttgatcatca tcgctgcata gtctttgacg tccttg
//
LOCUS       DATGKE010000179         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1099775, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000179 DATGKE010000000
VERSION     DATGKE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099775"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1056
                     /locus_tag="VKU83_03145"
     CDS             85..1056
                     /locus_tag="VKU83_03145"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HLI92572.1"
                     /translation="MRKFNSILVLLLVAGFARGQEINFSRVQDMTMWYNQSLKTDKQN
                     NLVLDYRSVSYGGQIAYNSVAAMFDMPLLSKDGKNKPHSGYFSMSAGAASDKSNAGIL
                     NNTLGMLGISYAVPISQHETYLALGFQAEYYQSNFNVAGISGAFGDQFDAYGPIEGQP
                     SADRLASGWSYGHFNANAGASVFSNGRYDKWYFGASVQQVNQPYSYEIKSDSTRLKPD
                     YGIQGGYKFTTATDDEVSLCMSMSWQGPAYRHYFDLSYTKAVPELVGGGAVGLGIGYR
                     YNDAIIPHLDLKYQKLLIGICYDVNISTISASGIARNGVELALRMDF"
     gene            1067..2608
                     /locus_tag="VKU83_03150"
     CDS             1067..2608
                     /locus_tag="VKU83_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI92573.1"
                     /translation="MMKVMTCLIGCFLFALTLKAQNTASPADTTRPAVDSAKQTSDTA
                     RMVAMLKDSGVVRPAASAGDTTSPATNSGATGSVIPPAGDPATPKLQSAAVNSDAPGQ
                     ASGNQGSKDTTADPSAAPASRQLDTRWFISPLLKLQFQDFAMLEKNREGYLSNANTLP
                     FFSRGNASFAASAYKNLTGRLSVSADLGLSFGHVTSNQVQISQTKSQTFNLLNATVYY
                     HLLPATYRLQPYVEVGINDIINSASYLTVPMGIGAKFTSRKVMALAEVSYGEAIGKNI
                     ANTTMYSLGVYIPIGGKKHKQLDTDDNPYNKTTKEDKKKDTTRPNGTVINNIYVTINM
                     DSVLRAKGLLNDDGSPRRDGADDADGSSGHSRRQRRGLRNFDVDDFSDNDYKMDSLDG
                     KPAIRFVVYFEFNDFGLNSSAFESIDKVIGHLRRTPNQFTVEIKGYTDSVGNDHYNNW
                     LSRERAKMVLKYMNSRGIPAELMQAKAYGKDDPVADNSDPSKAWLNRRAEIIVHARES
                     LANDK"
     gene            2692..>2881
                     /locus_tag="VKU83_03155"
     CDS             2692..>2881
                     /locus_tag="VKU83_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92574.1"
                     /translation="MLNLNTAALWGHLPANIKIRLIDEHGNLIDPLQLKEVDYLSRFQ
                     NKCVVNKIFHQMMADNRSG"
BASE COUNT          705 a          786 c          783 g          607 t
ORIGIN      
        1 tagcaaaagg tatgacttcg ctggtccggt aacgggagga acgcgtagtc cgaaaataag
       61 aaactattaa aatccaatat cccgatgagg aagttcaatt ctatcctggt tctcttactt
      121 gtcgcgggct ttgcccgggg acaggagatc aatttctccc gggtgcagga tatgaccatg
      181 tggtataacc agtccctcaa gacggataag cagaacaacc tggtgttgga ttatcggagt
      241 gtttcctacg gcgggcagat cgcctacaat agtgttgcgg cgatgttcga tatgcccctg
      301 ctgtccaagg acgggaagaa caaaccccat tcgggctatt tcagtatgag cgccggcgcc
      361 gcttccgata agtccaatgc gggcattctg aacaatacgc tgggaatgct gggcatttcc
      421 tacgcagtcc cgatcagcca gcacgaaacc tatctcgcgc tcgggttcca ggcagaatac
      481 tatcagagta atttcaatgt cgcagggatc tccggcgcat ttggcgacca gttcgatgcc
      541 tacggcccca ttgagggtca gccgagcgcc gaccggctcg cttccggttg gtcttacggc
      601 catttcaacg ctaatgccgg agcatcggta ttcagcaacg gcaggtatga caagtggtat
      661 ttcggcgctt ccgttcagca ggtcaaccag ccttactcgt atgagatcaa atccgattcg
      721 acgcgcctta agccggatta cggcatccag ggcggatata aattcaccac ggcaacggac
      781 gacgaagtgt ccttatgcat gtcgatgagc tggcagggcc cggcctatag gcactatttt
      841 gacctgtctt acaccaaagc cgttcccgag ctcgttggcg gcggcgcggt ggggctggga
      901 attggctacc ggtacaacga cgccattatc ccccatctcg atctgaaata tcaaaaattg
      961 cttataggta tttgttatga cgtaaatatt tcgacgatca gtgcgtctgg catcgcgcgg
     1021 aatggcgtgg agctggcgtt acggatggat ttttaaaatt aaccccatga tgaaggtaat
     1081 gacttgtttg attggttgct tcctgttcgc cctcacgctg aaggcgcaga acaccgccag
     1141 ccctgcggat accacccgtc cggcagtgga ttcggccaaa caaacatccg atacggcgag
     1201 gatggtggcc atgctgaaag acagcggcgt cgtgcgaccc gcggcttcgg ccggtgatac
     1261 gaccagcccg gccacgaatt ccggcgcaac gggatcagtc attccaccgg ccggcgatcc
     1321 ggcgacccca aaactacaga gcgctgcggt gaattcggat gcgcctggtc aggcttcggg
     1381 gaaccagggc agcaaggata ctacggcaga cccttcggcc gcgccggctt cccggcagct
     1441 ggacacgcgt tggttcatct cgccgctcct caagctgcag ttccaggatt ttgccatgct
     1501 ggagaagaac agggaagggt acctgtcgaa cgcgaatacg ttgccgttct tctcccgggg
     1561 caacgcgtct ttcgcggcat cggcatataa gaacctaacg ggccgcctgt ccgtcagcgc
     1621 ggacctcgga ttgagttttg ggcatgtgac ttcgaaccag gtacagatca gccagacaaa
     1681 gtcccagacc ttcaaccttc tgaacgccac ggtctattat catttacttc ccgctactta
     1741 ccgcctgcag ccgtatgttg aagtggggat caacgatatc atcaacagcg cctcgtatct
     1801 gaccgtgccg atgggcatcg gcgccaagtt cacttcgcgc aaggtgatgg ccctcgccga
     1861 ggtcagctat ggcgaggcca tcgggaagaa catcgccaac actacaatgt attccctggg
     1921 tgtttacatt ccgatcggcg gaaaaaagca caagcagctt gacacggacg acaatccgta
     1981 caacaagaca actaaggagg acaagaagaa ggacacgacc aggccgaacg gcaccgttat
     2041 caacaatatc tacgtgacga tcaatatgga ctccgtgctg agggcaaagg gtctcctgaa
     2101 cgatgatggc tctccacgcc gcgacggggc ggatgatgcc gacggtagtt cgggccacag
     2161 ccggaggcag cgccgcggcc tgcgtaactt cgacgtcgac gatttcagcg acaacgatta
     2221 taaaatggat tcccttgacg ggaagcccgc catccggttc gtcgtctatt tcgaattcaa
     2281 cgacttcggc ctaaactcca gcgcatttga aagcattgac aaggtcattg gccatctgcg
     2341 caggaccccg aatcagttta cggtagagat caagggttat accgattccg tcggtaacga
     2401 ccactacaac aactggctgt cccgggagcg cgcgaaaatg gtgttgaaat acatgaacag
     2461 caggggtatt ccggccgaac tgatgcaggc caaggcttac gggaaagatg atcccgtagc
     2521 ggacaacagc gaccccagca aggcctggct taaccggcgc gcagagatca tcgtccacgc
     2581 gagggaatcg ctggccaacg ataaatagtt tttaaaaaag acagcacgat aatggtccgg
     2641 caggacagat attgtgctat tattgctatc caaattaaaa tccgagaatc catgttgaac
     2701 ctcaacacag ctgccttgtg gggccatctc ccggccaata tcaagatccg cctgatcgat
     2761 gagcacggca atctgattga cccgctccaa ctgaaagaag tcgattacct ttcccgtttt
     2821 caaaacaagt gcgtcgtgaa caagatattt caccagatga tggccgataa ccggtcgggg
     2881 g
//
LOCUS       DATGKE010000180         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_61677, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000180 DATGKE010000000
VERSION     DATGKE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61677"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="VKU83_03160"
     CDS             <1..2041
                     /locus_tag="VKU83_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLI92575.1"
                     /translation="RFGHFRVSTISRLANQDSVIADGVMNGNAEVKNVTTKPVFTSKL
                     GVENLSYKADTLGDLTLNVNNEKANALTADIGLQGKNNDIAVSGDYYTGNSKMDLRLD
                     LRRVNLAAFTGAAAAVVDNMKGDLMGQLTIQGTVDKPAVRGSLHFDSAVITPAISGEA
                     LKVSGDEIGFDEDGFNFSQFTLRDSAGNKLILDGNVFTKDYHSFAFDLSLDAQNFRLV
                     NEPENSSRPFYGQLNLDAAINLEGDMKAPAVDGTIRVNKNTNFFYNLPTDNPEVGDRV
                     GVVRFVDHRTGDTLIDRKALALHARNSQIKGIDVSLNLITDTSAALNVVVDPHTGDEL
                     DVRGNSNLVFQLEKSGKMDLTGSYEVAGGFYKMSFDVLKRKFSIEPGSVVTWTGDPTS
                     ATVNLSATYTALAPSIDLVSNVISDLPQLERNKFQQKLPFLVTLKLNGDLLKPDIKFD
                     ISLPTTTLTLWPDVDERLSELRIHQSEMNEQVFALLLLGRFVGQDPLASAAGGGSTPG
                     NLALSSAGQIVTNQMDQAAASLIKPVDVHFNLINQQDFSTGYEIDYTELDVTLSKHLM
                     QDRLRASVGSSFDVVGTGAPQQAPSNLAGDVDVDYKLSKDGRYQVHAYRQNQYEAVVL
                     GQVIETGVGFIFLFNYDKFSEIWHRSKRLTIEPRKVSNHGTTSGGTSSSGTTSQR"
     gene            2038..>3228
                     /locus_tag="VKU83_03165"
     CDS             2038..>3228
                     /locus_tag="VKU83_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92576.1"
                     /translation="MTVISRILRFFIATVLFVTALASCNESKYLGENQVLYSANKTEI
                     HSSVPMTKKERNKWNEEMEGYLRPKLNSKILGIRVKLWIYNVAGQTTKKKGFKHWLKY
                     KVGEAPVLASPAILANNAQVLQGHLENKGFFHDTVIVTTFVKDKHLTATYSAQVGPRY
                     VIGKINYPDDSDLISNRIDTLQRHSLLKTGDYYDFETIKAERVRIDNRLKNRGFYYFL
                     PDYLLVDLDTAVGNHRVDMFMRLKDEMPDPAREVYHIDSITVYVNPGLKVDTDRKQAF
                     VTPEGYRVIDSTKFLRPKVFSQTLLIKPNNIYKEDDHNSSLSRLVSLGVFQFVKARFE
                     PASAADTNTGLNLYYYLTPAQRKQLRFEVTALTRSDNTTGTELALDWRHRNLFRGAEL
                     FNIRL"
BASE COUNT          781 a          912 c          874 g          661 t
ORIGIN      
        1 ccgctttggc catttccggg taagcacgat cagccggctc gccaatcagg actcggtgat
       61 cgccgacggc gtgatgaacg gcaacgcgga ggtgaagaat gtaacgacaa aaccggtatt
      121 cacttcgaag ctcggggttg aaaacctgtc ctacaaggcc gacacgctcg gcgacctgac
      181 gctgaatgtc aacaacgaga aggccaacgc gcttaccgcc gacatcggac tgcagggaaa
      241 gaacaatgac atcgccgtct cgggggatta ttacaccggc aacagtaaaa tggacctccg
      301 tcttgacctt cggcgggtca accttgcggc tttcacgggg gcggcggcgg ccgtcgtgga
      361 caatatgaag ggcgacctta tgggtcaact aaccatccag ggaactgtgg acaagccggc
      421 ggttcgcgga tcccttcact tcgacagcgc cgtgatcacc ccggccattt ccggggaagc
      481 gctgaaagtt tccggggacg agatcggttt cgatgaggac gggttcaact tcagtcagtt
      541 cacgctcagg gattcggcgg ggaacaagct gatcctggac ggcaatgttt ttacgaagga
      601 ctaccatagc tttgcttttg acctctcgct cgacgctcaa aatttccggc tggtcaatga
      661 accggagaac agcagccggc cattctacgg ccagctcaac ctcgacgccg ccatcaacct
      721 cgaaggcgat atgaaagcgc cggcggtcga tgggacgatc agggtaaaca aaaacaccaa
      781 tttcttttat aatttgccga cggacaaccc cgaagtgggc gaccgggtag gcgtggtccg
      841 tttcgtcgac caccgcaccg gcgacacgct gatcgacagg aaagcgctgg ccctgcatgc
      901 ccggaactcg caaatcaaag gtatcgacgt cagcctgaac ctgatcacgg ataccagcgc
      961 agctctcaat gtcgtggtcg acccgcatac gggtgacgaa ctggatgtcc ggggcaacag
     1021 caacctcgtc ttccagctgg aaaaaagcgg taagatggat ctcaccggtt cctatgaagt
     1081 cgccggcggc ttctataaga tgtccttcga tgtgctgaaa cggaaattca gcatcgagcc
     1141 gggcagcgtc gttacgtgga ccggcgatcc cacctccgcc accgttaact tgtcggccac
     1201 ttacaccgcg cttgcgcctt ccattgacct ggtgtccaac gtcatctccg atcttccgca
     1261 gctcgagcga aataaatttc agcaaaagct gcccttcctc gtcacgctca aattgaatgg
     1321 cgatctgctg aaaccggaca tcaagttcga tatttcgctg ccgacgacga cgctgacgct
     1381 ttggccggac gttgacgagc gcctttccga gttgcggatc catcagtcgg aaatgaacga
     1441 acaggtgttt gcactgctgc tccttggccg ctttgtcggg caggatcccc tggcctcggc
     1501 ggcaggcggc ggcagtacgc cgggcaacct ggccctgtcc agcgccggcc agatcgtgac
     1561 gaaccagatg gatcaggcgg ctgccagcct catcaaaccg gtggacgtac actttaatct
     1621 catcaatcag caggacttca gcacgggcta tgagatcgac tacaccgaac tcgacgttac
     1681 gttatccaaa catttaatgc aggaccggct ccgggccagc gtaggcagca gctttgacgt
     1741 cgtgggtacc ggcgcacccc agcaggcccc gtccaatttg gcgggcgatg tcgacgtgga
     1801 ctacaagctt tcgaaagacg gccggtacca ggtccacgcc tatcggcaaa atcaatacga
     1861 agccgttgtc ctcggtcagg tgattgaaac gggcgtcggc ttcatctttc tgttcaatta
     1921 cgataagttc agcgagattt ggcatcggtc gaaacggttg acgatagagc caagaaaggt
     1981 atccaatcac ggcactacat ccggcggcac aagttccagc ggcacaactt ctcaacgatg
     2041 acagtaattt cacgcatcct gcggtttttc attgcgaccg tgctattcgt gacggccctg
     2101 gcttcgtgca atgagtccaa atacctggga gaaaaccagg tattgtattc ggctaataag
     2161 actgaaatcc actcttccgt gccgatgaca aaaaaagagc ggaacaaatg gaacgaggaa
     2221 atggaaggat atctgcggcc gaagctcaac agcaagatcc tcgggatacg cgtcaagctg
     2281 tggatctata acgtcgccgg acagacgacg aagaaaaagg gattcaaaca ctggttgaag
     2341 tacaaggtgg gcgaggcgcc ggtactggcg agcccggcta tcctggccaa caatgcccag
     2401 gtgctccagg gccatcttga gaataagggt ttttttcacg atacggtcat cgtgaccact
     2461 ttcgtaaagg ataaacacct caccgcaacc tattcggccc aggtcgggcc acgctatgtc
     2521 atcgggaaga tcaactaccc ggatgattct gatttgatca gcaaccggat cgataccctg
     2581 cagcggcatt ccctgctgaa aacgggtgat tattatgatt tcgagacgat caaggcggag
     2641 cgggttcgta tcgacaaccg gctgaagaac cggggctttt attatttcct tcccgactat
     2701 ctgctggtcg acctcgacac cgccgtcggc aaccatcggg tggatatgtt catgcgactg
     2761 aaagatgaga tgcctgatcc cgcccgcgag gtgtatcata tcgacagcat tacggtatat
     2821 gtcaaccccg gacttaaagt cgacacggat cgcaaacagg cctttgtaac accggaagga
     2881 tatcgcgtca tagactcgac taaatttcta aggcccaagg tcttcagcca gacgcttctc
     2941 atcaagccga ataatattta taaggaagac gatcacaatt cctccctcag ccgcctggtg
     3001 agcctcgggg tcttccaatt tgtgaaggcc cgatttgaac cggcctccgc ggcggatacc
     3061 aataccggat taaacctcta ctactacctg acgcccgcgc agcgaaagca actccgtttc
     3121 gaggttacgg cgcttacgag gagtgacaac accaccggta ccgaattggc gctcgattgg
     3181 cggcaccgga acctcttccg gggcgcggaa ttgttcaata tccggctc
//
LOCUS       DATGKE010000181         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1410388, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000181 DATGKE010000000
VERSION     DATGKE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410388"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..522
                     /locus_tag="VKU83_03170"
     CDS             268..522
                     /locus_tag="VKU83_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLI92577.1"
                     /translation="MQVVNFTEFRKNLAGNLNLVNDSGDMVVVSRSKGKNVVVMSLED
                     FNSIQETLHVVSTPANQKRLDEALAEMRAGKTFRHKLSDN"
     gene            522..785
                     /locus_tag="VKU83_03175"
     CDS             522..785
                     /locus_tag="VKU83_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HLI92578.1"
                     /translation="MQLVWQTAAWEDYLSWQETDRSVVRKINSLIKACDRNPFKGAGK
                     PEPLKGLYAGCWSRRIKGDHRLLYAVRNQYLHILQCRFHRESP"
     gene            883..2304
                     /locus_tag="VKU83_03180"
     CDS             883..2304
                     /locus_tag="VKU83_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HLI92579.1"
                     /translation="MRKTCLLISLFASLILILPANIHAQVKKKLRVAVLTDIEADPDD
                     TESLIRFLTYCNEWDVEGLIATTSIHQKTRTAPESILKVLDAYAKVQPNLLKHEPGYP
                     TADFLRARVKHGLAVYGMEGVGAGHDSQGSDWIVDILKKPDDRPVWFCVWGGPNTLAQ
                     ALWKIKSTLPQDEAEKIYKKVRIYTISDQDDSGPWIRKTFPNLFYIVSPGYNYAHATW
                     LGMSFGMPGSNKEVVSAEWLARNIQEGHGPLGAAYPDVAYGMEGDTPSFLDLINNGLN
                     DPEHPDYGGWGGRYEFYLPPFEDSNTGPFSRPGWPKDEPESRPIWTNAVDSVLVDKLG
                     YASIQATIWRWRTEYQNDFAARMAWTTKRYRECNHPPVPHLGMPARFTVKSGEQFHLS
                     AAGTTDPDGDSMSYLWFQYPEAGTYKGRVSFAPYAANLYDVPVTAPVVDSPATIHFIL
                     KVTDKGTPALTRYKRVIVTVLPK"
     gene            2360..2872
                     /locus_tag="VKU83_03185"
     CDS             2360..2872
                     /locus_tag="VKU83_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92580.1"
                     /translation="MRLSFIAAVTLLLAAACTHKRTLTEQLTSTLSSHLRRLDPVAVV
                     DSVHILWTVPVNERFGRVVDDTVYIREYSRLKTELAGAQQRNEKDSIAFLHYEIAVME
                     KEIDSISKAVAKGDTTDRFGTLIGCAYYLRSADRTRPDSTMLFVDSTNTLRFTEFLDS
                     SLARTIRHLR"
     gene            2922..3725
                     /locus_tag="VKU83_03190"
     CDS             2922..3725
                     /locus_tag="VKU83_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLI92581.1"
                     /translation="MSDELDPYIRRWGLLPDGEPFTTFSGFLQPVRFNGARCMLKVAR
                     REKDRRGGELLVWWSGEGAAKVLVHDQRAILMERATGASSLAEMARSGQDDQATRILC
                     DVVLRLNSHKAPFPDQLVPLDQWFGDLPVAAAKYGGTFIKCAAVAADLLSNQNDFAPL
                     HGDIHHGNVLDFGDRGWLAIDPKGLIGERGFDYANIFCNPDPAIATKPGRLQRQASLA
                     ADAARLEWVRLLQWIVAWAGLSAAWCIEDGQEPQHALEVAKLALAALER"
     gene            3817..4509
                     /locus_tag="VKU83_03195"
     CDS             3817..4509
                     /locus_tag="VKU83_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92582.1"
                     /translation="MRILCCLGLFIFLSLPFTTHAQVSIGIQAGPDFAWLVNAVEGSN
                     GSTSITTQKSGSVTQIYGGVYVDIPLDSAGGMFYLRPGIEYLGAGGQMNANADYYNSN
                     GFLPSTKYSLRYVDVPFQFVYSPEMDFGKPWLGLGLYGGALVGGTVATQGSSSQPVKI
                     GNNASDNFKRFDAGYVFSLGLMTKSGFLFGADYQHGFVRAVPDARLNSSQSRLLTRNS
                     VWDLHVGWVFRL"
     gene            4596..>5146
                     /locus_tag="VKU83_03200"
     CDS             4596..>5146
                     /locus_tag="VKU83_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HLI92583.1"
                     /translation="MGNTLVKNAPANPRFVHLPLEEIKAGVHFVPGGLSHEEAEARLE
                     QYGFNEVAREKPVSPWRRLLTNLKNPLVILLSILGIVSWFTGDVPGTIVIFIMVVLGI
                     VLRYVQEMRADKAAEKLRAMVSTHATVCRDGKDMELPLKMIVPGDIIRLGAGDMVPAD
                     VHVLAAKDLFINQAALTGEAMPV"
BASE COUNT         1138 a         1438 c         1438 g         1132 t
ORIGIN      
        1 atgtaaaggt tatccccatt attaccattt gcagggggtg gtcatggccg ctaaaaatag
       61 ccaaaatagg gctgatcgcc gttcttgtct ttccggtccg gcattcggga cgcgccgccg
      121 tcgtaacatc aaacccgtcc tggtcgtcca acagatatca gttgagattt atattctctt
      181 gtgtaaaagc gaacggtcca tttccatgga ccgttttaat tttcggccaa aaaaccctaa
      241 tttagtactt aaataagtac ttattacatg caagttgtca attttaccga attccggaag
      301 aacctcgcag gtaatctcaa ccttgtcaat gacagcggcg acatggtggt cgtttcgcgt
      361 agtaagggga agaatgtcgt cgtcatgtcg ctcgaggact tcaattccat ccaggaaacg
      421 ctgcacgtcg tgagcacccc ggccaaccaa aagcggctcg acgaagccct ggcggaaatg
      481 cgcgccggga aaacgttcag gcataaacta agcgataact gatgcaactg gtttggcaaa
      541 ccgccgcctg ggaggattat ctgtcctggc aggagaccga cagaagtgtc gtccgtaaga
      601 tcaacagcct gatcaaggcc tgcgaccgca atccgttcaa aggtgccggc aagccggagc
      661 cgcttaaggg tttgtatgcc ggctgctgga gccgccgcat taagggtgac caccgcctgc
      721 tttacgccgt gcgcaaccaa tacctccata tcctgcaatg ccggttccat cgcgaaagcc
      781 catagccgtc ggggccaccg ctttttgcta tttacggtta ctttggtgaa ttttctttta
      841 tttgcatatc accctgatgc cgggtgcgga aactcccgtt atatgagaaa gacctgtctc
      901 ctgatttccc ttttcgcctc gctcatcctg atcctgccgg ccaatatcca tgcccaggta
      961 aagaagaaac tgcgcgtggc cgtcctgacg gatattgaag ccgaccctga cgataccgag
     1021 tccctgattc ggttcctgac gtactgcaac gaatgggacg tggaaggcct catcgcgaca
     1081 acttccatcc atcagaagac gcgcacggcc cctgaaagca ttctgaaggt gctcgatgct
     1141 tatgcgaagg tgcaacctaa tctcctgaag catgagccgg gatatccgac agcggacttc
     1201 ctgcgggccc gggtaaagca tgggttagcg gtctatggca tggaaggggt aggagcgggg
     1261 catgattcgc aggggtccga ctggattgtc gatatcctca aaaaacccga tgaccggccg
     1321 gtttggtttt gcgtctgggg cggccccaat accctggccc aggcactctg gaaaataaag
     1381 agcacccttc cgcaggacga ggcggagaag atctacaaga aagttcggat ttataccata
     1441 tcggaccagg acgacagcgg tccctggata cgcaagacct ttcccaacct gttttatatc
     1501 gtatcacccg gatacaatta tgcgcatgct acctggctcg gaatgtcctt tggaatgccc
     1561 ggttccaaca aggaagtagt gtcggccgaa tggcttgccc gcaatatcca ggagggccac
     1621 ggcccactgg gcgccgccta tcccgacgtt gcctatggga tggaagggga tacccccagt
     1681 tttctggacc tgatcaacaa cgggctcaat gacccggaac accctgatta tggcggctgg
     1741 ggcggccggt acgaatttta tctgccgcca tttgaagact ccaatacggg gccttttagc
     1801 cggcccggtt ggccgaagga cgaacccgaa tcgcgcccga tctggaccaa tgctgtcgat
     1861 tcggtacttg ttgataaact gggctatgcc agtattcagg ccaccatctg gcgctggcgc
     1921 acggaatacc aaaacgattt tgccgcccgc atggcctgga cgacgaagcg ttacagggag
     1981 tgcaaccacc cgcccgtccc tcacctcggc atgccggcac ggtttaccgt taagtcgggc
     2041 gagcagttcc acctgagcgc cgccggcacc accgaccccg acggagattc gatgagctat
     2101 ctctggttcc agtatcccga agcgggaaca tacaagggac gcgtcagctt tgcgccttac
     2161 gccgccaacc tgtatgatgt tccggtaacc gcgcccgtag tggacagccc cgcgaccatc
     2221 catttcattc tcaaagtgac ggataaaggg acgcccgccc tcacgaggta caagagggtg
     2281 atcgtaacgg ttttgccgaa atagcccagg actttgatag cggggagttt gtcgtacctt
     2341 ggttgaaaac cgggttgcca tgagactaag cttcatcgcc gccgttaccc tgctgctggc
     2401 cgcggcttgc acccacaaaa gaacgctgac cgaacaactg acctccaccc tctccagcca
     2461 tctgcggagg cttgatccgg tcgctgtcgt cgactcggtg cacattctgt ggaccgtccc
     2521 cgttaacgag agattcgggc gggtggttga cgataccgtt tatatccggg aatacagcag
     2581 attgaagaca gagctggcag gggcccagca acgcaatgaa aaggatagca tcgccttcct
     2641 ccactatgag atcgcggtca tggaaaagga gatcgactcg atttccaaag ccgtcgccaa
     2701 aggcgatacg acggacagat tcggcaccct gatcggctgc gcctattacc tgagaagtgc
     2761 cgaccgaacc agacccgaca gcaccatgct cttcgtggat tcgacgaata cgctccgatt
     2821 caccgaattt ctggactctt ccctggccag gaccatccgt catctgcgat gaccggctta
     2881 ggttatcggg ggttcgatat ccggatttca acacgcttta aatgtctgac gagcttgatc
     2941 catacattcg ccgctggggg ctgcttccgg atggagaacc cttcaccacc ttctcgggat
     3001 ttctgcagcc cgtacggttc aacggcgcga gatgtatgct caaggtagcc cgccgggaaa
     3061 aggaccggcg cgggggtgaa ctcctggtct ggtggagcgg tgagggcgcc gcgaaagttc
     3121 tggtccacga ccagcgcgcg attttgatgg aacgggcaac cggcgcatct tccctcgctg
     3181 agatggcaag gagcggtcag gatgaccagg ccacccgcat tctttgcgac gtcgtcctgc
     3241 gcttgaacag ccacaaagcg ccctttccgg accagctggt tccgctggat cagtggttcg
     3301 gggacctgcc ggtcgcagcg gcaaaatacg gcggcacttt tataaaatgc gcggcggtgg
     3361 cggcggacct cttaagcaat caaaatgatt tcgctcccct ccacggcgat attcaccatg
     3421 gcaacgtgct ggatttcggg gatcgcggct ggctggccat cgatccgaaa gggttgatcg
     3481 gagagcgtgg cttcgactat gcgaatatct tttgcaaccc ggatccggcg atcgccacga
     3541 agcctggccg cttgcagcgg caggcttccc ttgcagccga tgccgcccgg ctcgaatggg
     3601 tcaggttgct gcaatggatc gtagcctggg ctgggctatc ggctgcctgg tgcattgagg
     3661 atgggcagga acctcaacat gcgctggagg tcgcgaagct cgctctggcc gcattggagc
     3721 ggtgagcgag cggggcatta ataccgtgtt aaaattgttg cattattttg tcaatccata
     3781 cattccgcta acttttgctt ttccaaaacg acacttatgc gtatcctttg ctgtctcgga
     3841 ttgttcattt ttctttcact accctttacc actcatgcac aggtttcgat cggcattcag
     3901 gcaggcccgg attttgcctg gctggtcaac gccgttgaag gaagtaacgg cagcacttcg
     3961 atcacaaccc agaaaagcgg atcagtaacc cagatatacg gtggcgtcta tgtggacatc
     4021 ccgctcgaca gtgccggtgg aatgttttat ctccggcccg gtatcgaata cctgggcgcc
     4081 ggaggtcaga tgaacgccaa tgccgattac tataactcca acggcttcct gcccagcacc
     4141 aaatattcgc tgcggtatgt cgacgtgcct ttccaattcg tttacagccc cgaaatggat
     4201 ttcggcaagc cgtggctagg gctcgggcta tacggcggcg cgttggtcgg cggcaccgtg
     4261 gcaacgcagg gcagcagctc gcagccagtg aagattggaa acaatgccag tgacaatttc
     4321 aaacggttcg acgccggtta tgtgttcagc ctcggtttaa tgacaaagtc cggctttctg
     4381 ttcggcgccg actaccagca cggcttcgtc cgcgcggtcc cggatgcccg gttgaacagc
     4441 agccagagcc ggctcctgac ccgcaactcg gtttgggatc tgcacgtcgg gtgggtgttc
     4501 agactttagt cagacccctt attgacggga atcagctgca gcgccggcaa aaatgccgat
     4561 ctttaaaaat tactgcttaa atctacctga ccgtcatggg gaatactttg gtaaagaacg
     4621 ctccggcgaa cccccggttc gtccatcttc cattggaaga gatcaaagcc ggagtgcatt
     4681 ttgtgcctgg gggactttcc catgaggagg ccgaggcaag actggagcaa tatggtttca
     4741 acgaagtggc cagggaaaag ccggtatcgc cctggcgtcg cctcctgacc aacctgaaaa
     4801 acccacttgt catcctcctc agcatattgg gcatcgtgtc ctggttcacc ggggatgtcc
     4861 caggaacgat cgtcatcttc atcatggtcg ttttggggat tgtattacgc tacgtgcagg
     4921 aaatgcgggc cgacaaggcg gcagaaaagt tgcgggcaat ggtgagtacc catgcgaccg
     4981 tttgccgcga cggcaaggat atggaactgc cattgaaaat gatcgttccg ggagacatta
     5041 tccggctggg ggcgggggat atggtgccgg cggacgtcca tgtgctcgcg gcaaaggatc
     5101 tgtttattaa ccaggccgcg ctaacaggcg aggccatgcc cgtcga
//
LOCUS       DATGKE010000182         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_992995, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000182 DATGKE010000000
VERSION     DATGKE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992995"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VKU83_03205"
     CDS             complement(<1..306)
                     /locus_tag="VKU83_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92584.1"
                     /translation="MFREIFLFEIRTRIRRPAVYLYFLSLFVFTCFAFSTGSLPVGER
                     EHINSPYLISFWCAAMSMMMTLVSSPVMGMALLRDIEFQTKDYYLTYPITRPGYFWGR
                     "
     gene            complement(318..1208)
                     /locus_tag="VKU83_03210"
     CDS             complement(318..1208)
                     /locus_tag="VKU83_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI92585.1"
                     /translation="MKLVIHSLCKTYANGVQALRDVSLTLGNGMFGLLGPNGAGKSSL
                     MRTIATLQEADSGTVFLDGLDVLKDKTRVRQLLGYLPQEFGVYPRIPAEQMLDHIARL
                     KGVMNAGERRDVVQGLLEKVNLSKDRRKHLGTYSGGMKQRFGIAQALIGDPKLIIVDE
                     PTAGLDPAERNRFYNLLSQLGENTIVILSTHIVEDVSTLCSNFAIICHGEVLYAGDPE
                     QAVRELEGAIYSKAIDQSQIKYFRDVYNVISTQLKGGKLHIRVLARDNVPEDGFLPAV
                     PNLEDVYFSNIATRVDVNTL"
     gene            complement(1211..1930)
                     /locus_tag="VKU83_03215"
     CDS             complement(1211..1930)
                     /locus_tag="VKU83_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92586.1"
                     /translation="MRTSIKLFLGSMGLLGAGLLAYDLQLNAAFRKADYTQPFYNYVP
                     LDFRDFDRIRLNSAASLNIYLVKGDYHVKLNPRMADLVKLQKEGGELVITAHFPDRVN
                     ALNYALYISCPTLKGIETSGEYYFRNQLVDTHIPEQLWYEPTVMKGFRVDSLQISEKD
                     AGTIVLQDDRIGVLRAMVGAGSSLRIDAGNVIGGGDIAVLNKGQLTLKDTTGLNINHH
                     LADSATLILSGEAAKKLLKLH"
BASE COUNT          454 a          587 c          512 g          469 t
ORIGIN      
        1 ccggccccag aaataacccg gccgcgtgat cggataagtc aggtagtagt cctttgtctg
       61 gaattcgata tcccgcaata aggccatgcc catgaccggg gaactgacca atgtcatcat
      121 catcgacatg gctgcgcacc agaaactgat caggtaaggc gagttgatat gttcccgttc
      181 gccgacgggc agcgaccccg tgctgaaggc gaaacaggtg aagacgaata gggaaagaaa
      241 atacagatag accgccggtc ttcgaatccg ggtccggatc tcgaacaaga agatttccct
      301 gaacatagca aaggtattta aagcgtattt acatctaccc gcgtagcaat attgctgaag
      361 tacacgtctt ccaggttagg cacggcaggc aagaagccat cttccggcac gttgtcgcgc
      421 gccagtaccc ggatgtgaag cttgccgccc ttcaactgcg tcgagatcac attgtagaca
      481 tccctgaagt atttgatctg ggattgatcg atggccttcg aataaatcgc tccctccagt
      541 tcccgcacgg cctgttccgg atcgcctgca tacaacactt cgccgtgaca aatgatcgcg
      601 aaattcgagc acagcgtgct cacgtcctcc acgatgtgcg tcgacaggat gacgatggta
      661 ttttctccca attgactcaa caggttgtag aagcgatttc gctctgccgg atcgagccct
      721 gccgttggct cgtccacaat gatcagcttc gggtcgccga tcagcgcctg tgcgatgcca
      781 aatcgttgct tcatcccgcc cgaataggtc ccgaggtgtt tgcgccggtc tttcgaaagg
      841 ttcacctttt ccagcaatcc ctgcacgaca tcccggcgct cgcccgcatt catcacgccc
      901 ttgagccgcg cgatatgatc cagcatctgc tcggcgggaa tcctcgggta gacgccgaac
      961 tcctggggca gataacccaa cagttgccga acccgcgttt tatctttcag cacgtccagc
     1021 ccgtcgagga aaaccgttcc ggaatcagcc tcctgcaagg tggcaatcgt gcgcatcagc
     1081 gaggatttgc cggccccgtt ggggcccaga agcccaaaca tcccgttacc cagggtcagc
     1141 gagacatccc taagcgcctg gactccgttt gcataggtct tacacaaaga atgaatgaca
     1201 agtttcatgg tcaatggagt tttagtagct ttttcgccgc ttcgccactc aggatcaggg
     1261 tggcgctgtc agccagatga tggttgatgt tcagcccggt ggtgtccttc aaggtaagct
     1321 gacctttatt caggacagct atatcgccac cgccgatgac gtttcccgca tcgatcctca
     1381 gcgaagagcc cgccccgacc atcgcgcgca gcactccgat ccggtcgtcc tgcagaacaa
     1441 tggttccggc gtccttttct gagatctgca ggctgtccac ccggaatccc ttcatcaccg
     1501 tcggctcgta ccagagttgc tccggaatat gggtgtccac caattggttc ctgaaataat
     1561 actccccgct tgtttcgatc ccttttaggg tggggcagga gatatacagc gcataattca
     1621 gggcattgac acggtccggg aagtgcgccg taatgaccag ctcgccgcct tccttttgca
     1681 atttgaccag gtccgccatg cgcggattga gtttgacgtg gtaatcgcct ttcaccaaat
     1741 atatattcag cgacgcagcc gaattcagcc ggatccggtc aaagtcccgg aaatccagcg
     1801 gcacatagtt ataaaaaggc tgcgtgtagt ccgcttttcg aaaggccgca ttcagctgca
     1861 ggtcataggc gagcagaccg gctcccagca gacccatcga acccaggaat agcttaatgc
     1921 ttgttctcat cttgtgattg ttttatggcc ggctgccggg ctacccatgc cgtatattgt
     1981 ttttgtatct cttccattga aatccccagc agatacacat tt
//
LOCUS       DATGKE010000183         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_933580, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000183 DATGKE010000000
VERSION     DATGKE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933580"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VKU83_03220"
     CDS             complement(<1..411)
                     /locus_tag="VKU83_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92587.1"
                     /translation="MGKERKGEEGGMKMDTGRSARRRMGRRGGRALRIVGFIAGIILL
                     LVAGFFIANAVVCRKVDEALRSLPASMTLRYGTLRANVLTGSIQLGSIEGRYMPAPGH
                     SHHLSVNHLAINGFSMIGWLTAHRMRINEIHAEGI"
     gene            complement(476..1360)
                     /gene="dapA"
                     /locus_tag="VKU83_03225"
     CDS             complement(476..1360)
                     /gene="dapA"
                     /locus_tag="VKU83_03225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLI92588.1"
                     /translation="MSIRDQLRGTGVALVTPFRSDNSVDFNALEKVIEHVLSGGVEYV
                     VTLGTTGETPTLSKDEKRQIASFTIEKVAGRVPVVVGIGGNNTAELVRELADFPLSKA
                     AAVLSASPYYSKPSQEGLYQHYKALAAASPKPLILYNVPGRTGRNLSAATTIRLAREV
                     KNIAGIKEASGDMAQCMEILRDKPGQFLVVSGDDALAMPQIACGMDGVISVAANAFPR
                     EFSDMVRSALKGDFKTAKAINDRLIAAYEIMFAENNPAGVKAAMAQQQLIGDYLRLPV
                     VPLSEGLQAKWREYLGKK"
     gene            complement(1488..2465)
                     /locus_tag="VKU83_03230"
     CDS             complement(1488..2465)
                     /locus_tag="VKU83_03230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HLI92589.1"
                     /translation="MPQAKNRQFLDFEKPIKELFEEIEKLKQTAEKTKVDLSDSIRKL
                     EEQVLEKRREITQHLSPWQKVQLSRHPDRPYTMKYIEKLCTNFVELFGDRNVKDDKAM
                     VGGFAQLDGRTVMMIGQQKGTNTKMRQLRNFGMANPEGYRKALRLMKLAEKFDKPIIT
                     LIDTPGAYPGMEAEERGQGEAIARNIYEMMRLQVPVVCVIIGEGASGGALGIGVGDKV
                     YMLENSWYTVISPENCSSILWRSWDQKEKAAEELKLTPDNMYKFGLIDCIIPEPIGGA
                     HWDYNEAAALVKPRLIEALNELSRMTPQERIAKRIEKFGKMGFWNEVPA"
     gene            2697..2770
                     /locus_tag="VKU83_03235"
     tRNA            2697..2770
                     /locus_tag="VKU83_03235"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2731..2733,aa:Asp,seq:gtc)
     gene            2943..>3808
                     /locus_tag="VKU83_03240"
     CDS             2943..>3808
                     /locus_tag="VKU83_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLI92590.1"
                     /translation="MKIPIKPLCEEKWARKDGVSAIYLQWFHDGEHRIFLNTGLAIPS
                     AYWNRKKQCVVENLPIGYGSALQLNDEIDRQLRLAQDLIKLARSQDIPQIGLYVKEKY
                     SPGLVLDCLAHLDFNLRSAYIPEIKKKKENFFKHMDDYIASKSKRVKKGTLTVYHSMK
                     VHLEAYENYRKEPITFASLDYQFYESFLDFLIFEYRLPRKKTPQYGLRVNTIGKTIKQ
                     FRTFINDRVKRKIIPSIDLTDWRIPAEETDAVYLSYEEIQSIYQLDLSENPDLVAFRN
                     WLVLACLTGLRFS"
BASE COUNT          854 a         1085 c          931 g          938 t
ORIGIN      
        1 aatgccctcg gcatggattt cattgattcg catccggtgg gctgtcagcc agccgatcat
       61 tgagaatccg ttaatagcca ggtgattcac cgagaggtgg tggctatgcc ccggggcggg
      121 catgtaccgg ccctcgatag atcccaattg gatggaaccg gtcagaacat tggcgcgcag
      181 cgtcccgtag cgcaaggtca tcgatgcggg gaggctgcgc aatgcctcat ccactttgcg
      241 gcaaacgacg gcattggcga taaaaaagcc ggccaccagc agcagtataa tgccggcaat
      301 gaaacccacg atcctcagcg cccgtccccc gcgccggccc attctcctgc gagcgcttcg
      361 gccggtatcc atcttcattc cgccctcttc ccctttcctt tccttgccca taaccatttc
      421 ccatacaaac gccctgcccg gagccgccgt tgccagcggg caccggttgt ccggctcatt
      481 ttttccccag gtactcccgc catttggcct gtagcccttc gctcaggggt accaccggca
      541 ggcggagata atccccgatg agttgctgct gagccatagc ggccttgacg ccagccgggt
      601 tgttttccgc aaacatgatc tcataggcgg cgatcaaccg gtcattgatg gccttggccg
      661 tcttgaaatc ccctttgagt gccgaccgca ccatatcact gaattccctg gggaatgcat
      721 tcgcggccac gctgatcacg ccgtccatac cgcaggcgat ctggggcata gccaaggcgt
      781 cgtcgccgct gaccaccagg aactgcccgg gcttgtcgcg caatatttcc atgcattgcg
      841 ccatatcgcc gctggcttcc ttgatacccg cgatgttctt tacttccctt gcgagccgga
      901 tggtggtggc tgcggacaga ttgcgccccg tcctgcccgg gacattatac aatatgaggg
      961 gcttcggtga agcggcggcc agtgccttat aatgctgata aagaccctcc tgcgaaggct
     1021 tgctgtagta cggactcgcg ctcaggaccg ccgcggcttt gctcaacgga aagtcggcca
     1081 attcgcggac aagctcagcg gtattattgc cgccgatacc cacaacgacc gggacccggc
     1141 cagctacttt ttcaatagtg aacgatgcta tctggcgctt ttcgtccttt gacaaggtcg
     1201 gggtctcccc ggtcgtgcca agcgtaacga catattccac gccgccggaa aggacatgct
     1261 cgattacttt ttccagcgca ttgaagtcta cgctgttgtc ggatcgaaaa ggagtgacaa
     1321 gtgctactcc ggttccacgg agttggtccc tgattgacat attcgattgt aataaagagt
     1381 gaatgtatgg ctgcgctggc cggcgaaagc cggaaacgca aatgttatca tgcggcctgc
     1441 ccggcttcgg gaacgatttc tgcggtgctt gttccgtcct tctgacgtca ggccggcacc
     1501 tcgttccaga agcccatctt cccgaacttc tcgatccgtt tggcgatccg ttcctgcggc
     1561 gtcatgcggg agagctcatt taacgcttcg atcagccgcg gcttcaccag cgcggcggct
     1621 tcattataat cccagtgcgc gccgccgatc ggctccggga tgatgcaatc gatcaacccg
     1681 aacttataca tattgtcagg cgtgagcttc aattcttccg ccgctttctc cttctgatcc
     1741 cagctgcgcc aaaggatgga gctgcagttc tccggcgaaa tgaccgtgta ccaggagttc
     1801 tccagcatat aaaccttgtc ccccacgccg atccccagcg cacccccgga ggcgccctcg
     1861 ccgatgatga cgcagacgac gggtacctga agccgcatca tctcgtaaat attccgggcg
     1921 attgcctccc cttgtccgcg ctcctccgct tccatgccgg gataagcccc cggtgtatcg
     1981 atgagggtga tgatcggctt gtcaaacttc tccgccagct tcattagtct tagtgccttt
     2041 cgatagcctt ccggattcgc cattccaaag ttccgcagtt gccgcatttt ggtattggtc
     2101 cctttttgct ggccaatcat cattaccgtc ctaccgtcga gctgcgcgaa accccctacc
     2161 atggccttgt cgtccttcac gttccggtcc ccgaacagtt ccacaaaatt cgtacacagc
     2221 ttttcaatat acttcatcgt gtatggccgg tcaggatgcc ggctcaactg caccttttgc
     2281 catggcgaca gatgctgggt gatctcacgc cgcttttcca acacctgctc ctccagcttg
     2341 cggatcgaat cgctaaggtc cacctttgtc ttttcggcag tctgcttgag cttctcaatc
     2401 tcttcaaaca actctttgat cggcttttcg aaatccagga actgtcgatt tttcgcttga
     2461 ggcataaata ttactgtaga ttaactgtta atcgcgcgcc gtttcgccca gcggcagcga
     2521 cccggagaac cagtgtttat ccggtttgat gcgtaaaatt aagggttaag gctttgttac
     2581 aaaaagattt accggggttt gttggggaaa atcaaatttc acctatcttt gccatcccga
     2641 aaaaacgcgc cggttcccgg ccttttgggc cacgcgccgg catttattcg gaggttggat
     2701 tggtagttca gttggttaga atgccgccct gtcacggcgg aggtcgcggg ttcgagtccc
     2761 gtccagtccg ctaccgcgaa ggatcacatc atgtgatcct ttttcattcg tacaggttcg
     2821 tacaagttac tccctctttt tcagacgatt ataacaatgg ccttttcgaa agccatttat
     2881 ctttacaagg tcatacaaca tcaaccgcga ataaaaacgc gaacaaaaaa tattggtgta
     2941 gtatgaaaat cccaattaaa ccgctatgcg aggaaaaatg ggctcgaaaa gatggcgtat
     3001 cagccatcta tctgcagtgg ttccacgacg gtgagcatcg tatctttctt aataccgggc
     3061 tcgccattcc gtcggcatac tggaaccgaa aaaaacagtg cgtcgtagaa aatttgccga
     3121 ttggatacgg ctcagcgtta cagctgaatg acgaaattga ccggcaattg cgactagcac
     3181 aggacctgat caagctggcc aggagtcagg atatccccca gatagggcta tacgtcaaag
     3241 aaaaatattc gccggggctg gttcttgact gcctggcgca tctggacttt aatcttcggt
     3301 cagcctacat accagaaatc aagaaaaaga aggaaaactt tttcaagcac atggacgact
     3361 acatcgcctc aaaatccaaa agagtgaaga aaggaaccct cactgtttac catagcatga
     3421 aagtccacct ggaagcctat gaaaattatc gcaaggagcc cattacattt gcttccttgg
     3481 actatcagtt ctacgagagt tttctggact ttctcatatt tgagtaccgg ttgcctcgaa
     3541 aaaaaactcc ccaatatgga cttcgtgtga atacgatagg caagaccatc aagcagttta
     3601 gaacatttat aaatgatcgt gtcaagcgaa aaatcattcc ttcaatagat ctaaccgact
     3661 ggaggattcc agctgaagag acagacgcag tctacctgag ctatgaagaa atccagtcca
     3721 tttatcagtt agacctgtcg gaaaatccgg atctggttgc ctttcgaaat tggctcgttt
     3781 tagcctgtct caccgggcta cgattttc
//
LOCUS       DATGKE010000184         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1519490, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000184 DATGKE010000000
VERSION     DATGKE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519490"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VKU83_03245"
     CDS             <1..1218
                     /locus_tag="VKU83_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLI92591.1"
                     /translation="VGVPGWTIVLWVLIAGAVLVLMRLGVRWFSLVRLRRKVTPIKGK
                     GMKIYQLDGPVIPFSFGKAIYINRHLHSAREWEDIILHEYVHIRQRHTIDIMIAELVC
                     VVNWYNPFAWLIRYSIRQNLEFIADQEVLDKGADPKGYQYHLLKVVGEPRYRLANNFN
                     FSALRERIVMMNKMRTPRVHLFKLLFLLPLVAVLLLAFRDRQKLPDRVPQASGRSHRV
                     KGDAAKARDGVRDTARDGLRDIAPAGLRDTVPDNGKSGGKPGGRMILNVHRDSLSAVS
                     KDLGNQPVHIIGGREVPADTLSKINPDSIASVDVLKDSAAMNSPAFKKYGKRAQNGIV
                     IIYLKGEKSPMCLVDGRETSWASAMRMDPGLIESVHVWKGAEAKKRYGDKGENGVVVI
                     KLKAKGPQTGVKP"
     gene            1373..1936
                     /gene="efp"
                     /locus_tag="VKU83_03250"
     CDS             1373..1936
                     /gene="efp"
                     /locus_tag="VKU83_03250"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLI92592.1"
                     /translation="MANTADISRGMIIKLDGSLYTVVEFGENKTARAAAKVWAKLKGV
                     DNTRSIEKTWNSGDTIYPVRVERKAYQFLYKDDTGYNFMDNETFEQIALQEHLIDAPQ
                     FLKEAQEVSVLFNTDTEQPMSVELPDKIVVQVTYSEPGIKGDTATRTLKPAKVETGAT
                     VNVPLFVNEGEMIRINTKTGEYVERVK"
BASE COUNT          553 a          494 c          661 g          499 t
ORIGIN      
        1 gtgggagtgc cgggatggac catcgttttg tgggtcctga tcgcaggcgc ggtgctggtg
       61 ctgatgcggc tcggggttcg atggttctcg ctggtccggt tgcggcgcaa agtcacgccg
      121 atcaaaggaa agggaatgaa aatataccag ctggatggcc cggtcattcc gttttctttc
      181 gggaaagcta tttacatcaa ccggcatttg cattcggcca gggaatggga ggatatcatc
      241 ctgcatgagt atgtccatat ccggcagcgg cacacgattg acattatgat cgccgagctc
      301 gtttgtgtgg taaactggta taatcctttt gcctggctga tccgctactc gatccgccag
      361 aacctggagt tcattgcgga ccaggaggtg ttggataagg gcgccgaccc gaagggatac
      421 cagtatcatc tcttgaaggt ggtgggcgag ccaaggtacc ggttggcaaa taacttcaac
      481 ttttccgcgc tgcgcgagcg catcgtgatg atgaacaaga tgcgcacccc gcgggtgcat
      541 ttgttcaagc tgttgttcct gctgccgctg gttgcagtgt tgttgctggc gttcagggac
      601 cgtcaaaaat tgccggatcg ggtgccgcag gcatcgggaa gatcccacag ggtgaaagga
      661 gacgcagcga aggctcggga cggcgtgagg gatacggctc gtgatggcct ccgcgatatc
      721 gctccggccg ggctgcgcga tacggtcccg gataacggga agtccggcgg caaacctggc
      781 gggcgcatga tcctaaacgt gcatcgggat tcgcttagcg ctgtgtcgaa ggatctgggc
      841 aatcagccgg ttcatattat cggcggcagg gaagtgccgg cagatacgtt gagcaagatc
      901 aacccagaca gcatcgcatc agtcgatgtt ttgaaggact cggcggcaat gaattcgccg
      961 gcatttaaaa aatacggaaa gagggcgcag aacggcatcg tcattatcta tctgaaggga
     1021 gaaaagtccc caatgtgcct cgttgatggg cgcgagacaa gttgggcctc cgcaatgcgc
     1081 atggatcccg gcctgatcga atccgtgcat gtctggaaag gagcagaggc gaagaaaagg
     1141 tatggggata agggcgagaa cggggtggtg gtgatcaagc tcaaggccaa aggaccacaa
     1201 accggagtga agccataggg ccgggacccg ggtcgctgca ttttggggaa tttactgtaa
     1261 atttgcaccc tccttcccgg aagcggttca tgctagtgtt tttagcggcc ggccggaaag
     1321 gagggtttct gtttttcccg gttaacggat ttaaaatttt ttaacagcgt ttatggcaaa
     1381 tactgcggac atcagtcgcg gcatgatcat caaactggat ggcagcttgt acaccgtcgt
     1441 ggagtttggc gaaaacaaga cggcaagggc tgcggccaag gtttgggcaa aattgaaagg
     1501 agtggacaat acgcgctcca ttgaaaagac ttggaacagc ggcgatacga tctatcccgt
     1561 acgcgtggaa agaaaagcgt atcaatttct ctataaggat gataccggtt ataatttcat
     1621 ggataatgag actttcgaac aaattgcctt gcaggaacat ttgatcgatg ctccccagtt
     1681 tctgaaggag gcgcaggaag tctcggtgct gttcaatacg gataccgagc agcccatgag
     1741 tgtggagttg cccgataaga tcgtggtgca ggtcacctat tctgaaccgg ggatcaaagg
     1801 ggacacggca acgcggaccc tgaaacctgc taaggtggaa acgggcgcaa cggtaaatgt
     1861 gccgcttttc gtcaatgaag gagaaatgat ccgcatcaac acgaagacag gcgagtatgt
     1921 cgaaagagtg aaataagtgc tccccggatt tatgtaagcg attgattctg aaaatttacc
     1981 cgtgtttttg ggtatatttg gctttaatcc ctattttagc cacccatata aaactgtaaa
     2041 ttcatctgat atggatttca aacagatcca ggagttgatc aaaatgatca acaagtccaa
     2101 cattggcgag gtgagtatcg aggagaaggg atttaggttg acgatcaagc aaaaggaaga
     2161 acccgttcag caaataatta ccgctcccgt cccggcagct ccggttt
//
LOCUS       DATGKE010000185         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_992889, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000185 DATGKE010000000
VERSION     DATGKE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992889"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VKU83_03255"
     CDS             <1..868
                     /locus_tag="VKU83_03255"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HLI92593.1"
                     /translation="TSSPDQMMPSHGVMVHGWLPETNPIEVVSPSGVCCAGMHAFKYA
                     HMAVMTGDINRAVATGSERFSASLLANFFEEEAQKLKELNENPFIAFDKEFLRWMLSD
                     GAAAFLLSDQPNQDGLSLRVEWIEGASYANEMETCMYMASEKQKDGTLKSYLEFTPEE
                     IIGKSIFSIKQDIKLLSDNIVPLGARKIKEIFDRKGLTPDDIDYFLPHMSSEFFKSKI
                     FEQIALHGASIPYEKWFVNLSTMGNVGAGSVYLMVHELFHSGKLKKGDRLLLLVPESS
                     RFSYMYAMLTAC"
     gene            880..1293
                     /locus_tag="VKU83_03260"
     CDS             880..1293
                     /locus_tag="VKU83_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92594.1"
                     /translation="MKKDEVPQDPGSLGKVTREICYATDDKGKYTTELSQGWEVKTGA
                     LDVAWQDITERVKAAGEKVLRGEASPLLYFMELKLMDAAVLAGYTGLWKWQVKRHLRP
                     AAFSRLSGRKLRRYAEVFEISVEELLHPNIHDTRI"
     gene            1277..>2027
                     /locus_tag="VKU83_03265"
     CDS             1277..>2027
                     /locus_tag="VKU83_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HLI92595.1"
                     /translation="MTPEFDHIQAAHCENGVTVSLLRNIGVDRITEPLAFGIGSGLFF
                     TYIPLLKVNNGPAFAFRTMPGLIFKRTCRALDIHIRRRRFRRKAEARAYLDECLEAGH
                     AVGCQVGVYYLPYFPKEYRFHFNAHNLIVFGKSNGRYLVSDPIMEHPSSLSDYELERV
                     RFARGVLAPRGQIYYPVPGKPVTDEMIRQGIRHGIKRNIRHMLHIPGPIAGISGIRYT
                     AGRIKKWRDLLGVKKAGVWLAQLVRMQEEIGT"
BASE COUNT          465 a          528 c          610 g          424 t
ORIGIN      
        1 tacctcttcg ccggatcaaa tgatgcccag tcatggcgtg atggtgcatg gctggctacc
       61 cgagacaaac ccgatcgagg tggtgtcccc gtccggcgtt tgctgtgcgg gcatgcacgc
      121 attcaaatac gcccatatgg cggtcatgac gggtgacatc aaccgggcgg tcgctaccgg
      181 atcggaacgg ttttccgctt ccctgctggc gaatttcttc gaagaagaag cgcagaagct
      241 gaaggagctg aacgaaaacc ctttcatcgc cttcgataag gaatttcttc gctggatgct
      301 ttccgacggc gccgccgcct ttctgctgtc cgaccagccg aatcaggacg gtttgagcct
      361 gcgggtcgaa tggatcgaag gcgcctccta cgccaatgag atggaaacgt gcatgtatat
      421 ggccagcgaa aagcagaagg acggaaccct gaagagttat cttgaattca ccccggaaga
      481 gatcatcggt aaatcgatct tcagcattaa gcaggacatc aagctgctga gcgataatat
      541 cgtacccctc ggcgccagga agatcaagga gattttcgac cggaaaggtt tgacaccgga
      601 tgacatcgat tatttcctgc ctcatatgtc gagcgagttc ttcaagagca agatctttga
      661 gcagatcgca ttgcatgggg ccagcatccc ttatgaaaaa tggttcgtta acctgagcac
      721 catgggcaac gtgggggcag ggtccgtgta tttaatggtg cacgaactat ttcactccgg
      781 aaaattgaaa aagggcgaca ggcttctgtt gctggtaccg gaaagctccc gtttctcgta
      841 tatgtatgcc atgctgacgg cctgttagaa agtacacaaa tgaaaaagga cgaagttccc
      901 caggatccgg gttcgctcgg caaggtcacg agggagatct gctatgcgac ggacgataag
      961 ggtaagtaca cgacggagct gagccagggc tgggaagtca aaacgggcgc cctggatgtg
     1021 gcgtggcagg atataacgga gcgggtcaaa gccgccggcg aaaaagtttt gcgcggcgaa
     1081 gcgagtccgc tgctgtattt catggagcta aagctgatgg atgcggcggt gctggccggg
     1141 tacaccggat tatggaaatg gcaggtaaag cggcatttgc ggccggcggc cttttccagg
     1201 ttatccgggc gaaagctgcg ccggtatgct gaagtattcg agatttcggt cgaggagctc
     1261 ctccacccca atattcatga caccagaatt tgaccatatc caggcagcgc attgcgagaa
     1321 cggtgtgacg gttagcctgc tcaggaatat aggcgtcgac cggatcaccg agccgctggc
     1381 attcgggatt ggatccgggc tgttctttac atatattccc ctgctcaagg taaataacgg
     1441 gccggccttc gcgttccgga ccatgccggg attgatcttc aaacggacct gccgggccct
     1501 cgacatccat atccgccgcc ggcggttccg ccggaaagcc gaggcgcgag cctatctcga
     1561 tgaatgtctc gaggccgggc acgccgtcgg ctgccaggta ggcgtttatt acctgcctta
     1621 tttcccgaaa gaataccgtt tccatttcaa cgcgcacaac ctgatcgttt tcggcaagtc
     1681 gaacggacgg tacctggtca gtgatcccat catggagcac ccgagcagcc tgtcggatta
     1741 tgagctggag cgggtgcggt ttgcgcgggg cgtattggcc ccgcggggac agatctatta
     1801 tccggtaccc gggaaaccgg tcactgacga gatgatccgg cagggtatcc gccacggcat
     1861 caaacgcaat atccggcata tgctccatat tcccggcccg atcgccggca tcagcggcat
     1921 ccggtacacc gcggggagaa taaaaaaatg gcgcgatctc ctgggcgtga aaaaggccgg
     1981 ggtctggctt gcgcagctgg tgcggatgca ggaggagatc gggacgg
//
LOCUS       DATGKE010000186         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1118492, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000186 DATGKE010000000
VERSION     DATGKE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118492"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VKU83_03270"
     CDS             complement(<1..226)
                     /locus_tag="VKU83_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92596.1"
                     /translation="MASIKQLQGILRLTYGLVPIVAGLDKFTNLLTHWVDYLGSNKSL
                     IPFDALVFMKIVGIIEIIAGIIVLVRPLIGA"
     gene            331..993
                     /locus_tag="VKU83_03275"
     CDS             331..993
                     /locus_tag="VKU83_03275"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLI92597.1"
                     /translation="MPDQPNVASSQITDKELIKRITGGEKELFEIIIRRYNKRFYRIG
                     MSILNNDQEVEEAMQAAYISAYEHLHQFENRAAFGTWLTRIMLNQCFKARRERSRTLS
                     VQALSENVINMKTPENVMASRELGRALENAISRLPEKYRVVFVLREIEDLSIQETAEV
                     AGIQASNVKVRLNRAKTMLRESLTDYMKESVYQFHLSRCDKIVGEVMGYLRSSLPSAR
                     IE"
     gene            complement(970..1704)
                     /locus_tag="VKU83_03280"
     CDS             complement(970..1704)
                     /locus_tag="VKU83_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI92598.1"
                     /translation="MEKPIKMIRCMIVDDEPLAREVIRRYIARVPFLQLIAECENAIR
                     AMAVVQQQGIDLAFVDIRMPELLGTEWIKMLRHPPRIVLTTAYAEYALEGYELDVVDY
                     LLKPIQFERFLKAVNKTVQQDQPDQPPAPHDPPENRKESYLYFRTDRKMVKVMLDEIV
                     YIEGMKNYIKIITDKGIVITKNSMTAIEAMLPGTAFIRTHRSYIVSRAKISSFTGECI
                     GIGKTEIPIGKLFKTNVMRSLDPGAR"
     gene            complement(1714..>2470)
                     /locus_tag="VKU83_03285"
     CDS             complement(1714..>2470)
                     /locus_tag="VKU83_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLI92599.1"
                     /translation="PEPYGSLSRHLPYDFLFALRITLNIAGFAVALKLLKHWYQKEFR
                     NNILQKEKLDAELQSLKAQLHPHFLFNTLNNIYSIAEDTSPQASEMLLKLSAILRYIL
                     YECNHSRVSLAQELTLIRDYLDLEKIRYSNLDLSVHLPADTGAYTITPLLLLPLVENC
                     FKHGASQLLEQPWIKIEAYIQDGWLLVKLINGRRPVEEKDTFTEGIGLSNLRKRLELL
                     YPGRHELNIITEQELFILNLSVELENKTDAEAR"
BASE COUNT          606 a          601 c          659 g          604 t
ORIGIN      
        1 acgcaccgat caggggccgt acgaggacaa tgataccggc tatgatctcg atgatcccga
       61 caattttcat aaacaccaat gcgtcaaacg gaataaggct tttattgctg ccgaggtaat
      121 caacccaatg cgtcaacaaa ttagtaaatt tgtccaatcc ggctacaatg ggtactaatc
      181 cgtacgtaag cctcaaaatg ccctgaagct gtttaatact tgccataaaa gtagattttg
      241 tttaaagatt ggagcaataa ctactcaaaa ggttacaaaa aatttcgtgg ccggtctgta
      301 acctttcggc accgaaaata tctaacgatc atgccagatc agcccaatgt cgcatcgagc
      361 caaataacgg acaaagagct cattaaacgc ataacgggag gcgaaaagga gcttttcgag
      421 atcattatcc gccggtataa caagcggttc taccggatag gcatgtccat cctcaataat
      481 gatcaggagg tggaggaggc gatgcaggcc gcttatataa gcgcctatga gcatcttcat
      541 caattcgaaa atcgcgctgc tttcggaacc tggttgacgc ggatcatgct caatcaatgc
      601 tttaaggcca ggcgtgaacg tagccggact ctatctgttc aggcactcag tgaaaatgtc
      661 atcaacatga aaacgccgga aaatgtaatg gccagcaggg aactgggccg ggcgctggaa
      721 aacgcgatct cccggctgcc ggaaaaatat cgcgttgtct tcgttctgag ggagatcgag
      781 gatctctcga tccaggagac ggccgaggta gccggcatcc aggcatccaa tgtgaaggtc
      841 cggctcaaca gagccaaaac catgctgcgg gaaagtctca ccgattatat gaaggaatcc
      901 gtttatcaat tccatctatc ccgatgtgac aagatcgtcg gcgaggtcat gggttatctg
      961 cggtccagtt taccgagcgc ccggatcgag tgatctcatc acgttggtct tgaagagctt
     1021 tccgataggg atctcggtct tgccgatccc gatgcattca cccgtgaacg aactgatctt
     1081 tgcccgggag acgatatacg atcggtgggt ccggatgaag gcagttccgg gaagcatcgc
     1141 ttcgatggct gtcatagagt tcttggtgat gacgatgcct ttatcggtaa tgatcttgat
     1201 gtaattcttc atgccctcga tatagacgat ctcatcgagc atgaccttga ccatctttcg
     1261 gtcggtccgg aaatagagat aggactcttt tctattttcg ggggggtcat ggggtgccgg
     1321 cggctggtca ggctggtcct gctgtaccgt tttattgacg gctttcagga accgttcgaa
     1381 ttggatgggt ttcagcaaat agtctaccac atcgagctca tagccttcca gggcatattc
     1441 ggcgtaagca gtcgtcagga cgatccgggg aggatgccgg agcattttta tccattccgt
     1501 gcccaggagt tccggcatgc ggatatcgac aaatgccaga tcgatgccct gttgctggac
     1561 gacggccatc gcccgaatgg cgttctcgca ttcggcgatc agctggagga agggcacccg
     1621 tgcgatatac cggcggatca cctctcttgc caggggttcg tcatcgacga tcatgcagcg
     1681 gatcatttta atgggcttct ccatacgttt tgcttaacgg gcttccgcgt cggttttgtt
     1741 ctccaattcc acgctgagat tgagaataaa gagttcctgt tcggtgatga tgttcagctc
     1801 gtgtctgccc gggtagagca gctccagcct tttccttagg ttgctcagcc cgatcccttc
     1861 cgtaaaggta tccttttctt ccacggggcg ccggccgttg atcaatttaa ccaggagcca
     1921 gccgtcctgt atatacgctt ctattttgat ccagggctgt tcgagcagct ggctcgcgcc
     1981 atgcttgaaa caattttcca ccagcggcag caataacaac ggcgtgatcg tataggcgcc
     2041 ggtatcggca ggcaggtgga ccgagagatc caggttcgaa taccggatct tttccaggtc
     2101 gagatagtcg cggatcagcg tcaactcctg ggcaagcgac acccgggagt ggttgcattc
     2161 atacaggata tagcgcagga tcgcggacag cttcagcaac atctccgaag cctgcggtga
     2221 agtatcctcc gcgatagaat aaatattatt cagggtgttg aataaaaaat gcggatgcag
     2281 ctgagctttc agcgattgca actcggcatc cagcttttcc ttttgcagga tattgttgcg
     2341 aaactctttt tggtaccaat gtttgaggag cttaagggca acggcgaaac cggcgatgtt
     2401 gagcgtaatc cggagagcga agaggaaatc gtagggcaag tgtctgctaa gcgaaccata
     2461 cggctcggga
//
LOCUS       DATGKE010000187         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_787780, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000187 DATGKE010000000
VERSION     DATGKE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787780"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VKU83_03290"
     CDS             complement(<1..1019)
                     /locus_tag="VKU83_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HLI92600.1"
                     /translation="MEIPLLPLIINPQATTILAIVILFLLLLSFVLSGSEVAFFSLTY
                     KDINILKTKQDASWRRIVSLLEDPKTLLASMLIANSIINIAIIILANFLIDEMVLLKQ
                     NFWFFEFLIKVILVAFVMILFGEVMPKIWATQNNLQFAYYTSGIIELVHLLFRRVSGW
                     AVSQSEGLERFFGSKKRGLIIEQLGNQIDKSVRIEASEGERNILKGIVKFGSITVRQV
                     MRARLDVCGAAYDLSFANLKRRFEELHYSRLPVYRSSLDDLAGMIHSRDLIGYLSQPD
                     NYEWHGLLRPIYFVHEHKPIEDLLQEFQQKRIHFAVVVDEFGGTSGIVTLEDILEEVI
                     GDIRD"
     gene            complement(1047..1484)
                     /locus_tag="VKU83_03295"
     CDS             complement(1047..1484)
                     /locus_tag="VKU83_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLI92601.1"
                     /translation="MRGVNRVMLIGNLGKDPDVQYLEGNIAVAKFSLATTETFKDRAG
                     KLISQTEWHTVVLWRGLAELAQKYLHKGSLVYIEGRLRTRSWEDKEGNKKFATEVVGD
                     NLIMLDKRTDGPAHPPIHHNEGMEGIGGTDAAPLGEPSEDLPF"
     gene            complement(1623..>2169)
                     /locus_tag="VKU83_03300"
     CDS             complement(1623..>2169)
                     /locus_tag="VKU83_03300"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLI92602.1"
                     /translation="NQAIMDFGATVCLPRNPLCPSCMQRAACEAQRNGWTTQLPVKEK
                     SVSKKQRWLYYFLVETSDGKVYIRQRTTKDIWEGLFELVLYETDRPAQFDEADIAASD
                     VVRQLFGKQVLTVRHISRVYRQELSHQTIQGRFLTVRLGKVISEMKGYQLVSRKQLAE
                     YPFPKFINGWLRDPAPAQTLF"
BASE COUNT          530 a          556 c          558 g          525 t
ORIGIN      
        1 tcatcgcgga tgtcgccgat cacctcttct aagatgtctt ccagggttac gatgccgctg
       61 gttcccccga actcgtcgac aactactgcg aaatggatcc ttttctgctg gaattcctgg
      121 aggagatctt cgatcggctt atgctcatgg acgaaataga taggccgcag aagaccatgc
      181 cactcataat tgtccggctg actcagataa ccgatgaggt cccggctatg gatcatgccg
      241 gccagatcat ccagactgct ccggtagacg ggcagccggg aataatgcag ctcttcgaaa
      301 cgccgcttga gattggcaaa cgacagatca taggcggcgc cgcagacatc gagccgggcc
      361 cgcatcacct gacgcaccgt aatgcttccg aacttgacga tccctttcag gatgttacgt
      421 tctccctccg acgcctcgat ccggaccgat ttatcgatct ggttgcccag ctgctcgatg
      481 atcaggccac gtttcttgct cccaaaaaag cgctccaggc cttcggattg acttacagcc
      541 caaccgctta cacggcgaaa cagcagatgg accagctcga tgatcccgga agtgtagtag
      601 gcgaattgca gattgttctg ggtagcccag atcttgggca tgacctcgcc gaaaaggatc
      661 atgacgaagg ctaccaatat gaccttgatg aggaactcga agaaccagaa gttctgtttg
      721 agcaggacca tttcatcgat caggaagttg gccaggatga taatggcgat attgatgatg
      781 ctgttcgcga tcagcatgga cgctaaaagg gtcttgggat cttccagcag cgagacgatg
      841 cgtcgccagg aagcatcctg tttggtcttt aggatattga tatccttata ggtaagcgaa
      901 aaaaaggcca cttcggaccc cgagagcaca aacgagagga gaagcaggaa aagtatgacg
      961 atcgccagga tggtggtagc ctggggattg atgatcaagg gcaacaaagg gatctccaag
     1021 attcaacagg tttcgttaat aatcggctaa aaaggaagat cttcggaggg ttcgccaagg
     1081 ggagctgcat cggttccgcc tattccttcc atcccttcat tatgatgtat gggaggatga
     1141 gccggtccat cagtacgttt atcgagcatg ataaggttat ctccaactac ttcggtggca
     1201 aattttttat tcccttcttt atcttcccag ctgcgggtcc tgaggcggcc ttcgatatat
     1261 acgaggctgc ccttgtgaag gtatttctgc gccagttccg cgaggccccg ccaaagtacg
     1321 accgtatgcc attcggtctg agagatgagc ttgccggccc ggtccttgaa agtttccgtg
     1381 gtggctaaag agaattttgc aacggcgata tttccttcca ggtattgaac atcagggtcc
     1441 tttcccaaat tacctattag catcacccta tttacgcctc tcatacatca aattttgaat
     1501 tgaaagcaaa tcacaataaa tcgtcttgga attcagcgcc aattaaaatt agctcatttc
     1561 agccaaaaag ataaattttt gatataagcc gtcgaagacc ggcttagtcc gaaggacaga
     1621 tgtcagaaca aggtttgtgc aggtgcggga tcccgtagcc aaccattgat gaatttggga
     1681 aatggatact ctgccagctg cttgcggcta acgagctgat agcctttcat ctcgctgatc
     1741 accttcccca aacggaccgt caggaaacgg ccctgtatgg tttgatggct gagctcctgc
     1801 cggtagaccc gtgagatatg cctgacagtg agaacctgct tgccaaacag ttgtcggaca
     1861 acgtcggacg cagcgatatc cgcctcatcg aattgcgctg gcctgtcggt ctcgtacagc
     1921 acgagctcga acaggccttc ccagatgtcc ttggtagttc gctgccggat gtaaaccttt
     1981 ccgtcggacg tttccaccag gaaataatac agccagcgtt gtttcttgct gacgctcttt
     2041 tcttttacag gcagttgcgt ggtccaaccg ttccgttgcg cctcgcaggc ggcgcgctgc
     2101 atgcacgatg ggcataacgg attacgcggc agacaaacag tggcgccgaa atccataatg
     2161 gcttggtta
//
LOCUS       DATGKE010000188         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1305406, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000188 DATGKE010000000
VERSION     DATGKE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305406"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VKU83_03305"
     CDS             complement(<1..1928)
                     /locus_tag="VKU83_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HLI92603.1"
                     /translation="METVAAIPKYNLTEDQEKRLILREYRALLRSLKPKLKIGDKELI
                     RSAFEMAAEAHKTMRRKSGEPYILHPLAVARICSEEIGLGVRSTICALLHDTVEDTDI
                     TLEDIEREFSGEIARIVDGLTKISNVVDVNASRQAENFKKILLTLTDDPRVILIKLAD
                     RLHNMRTLDSMKREKQLKIASETVYVYAPLAHRMGLYTIKTELEDLAMKYLEPEEYRE
                     IARKLAETKRERSRYINEFVKPLKDKLEKAGFNFDIYGRPKSIHSIWNKMRKKGVAFE
                     EVYDLFAIRVILNSTVEKEKEDCWKVYSMITDEYNPSPERLRDWLSNPKSNGYEALHT
                     TVMGPQGKWVEVQIRSRRMNEIAEKGLAAHWKYKEGAADESRFDKWFQQIREVLNTED
                     NDSIDFLQDFKTSFLAEEIYVYTPRGDVKMLPVGSTALDFAFSIHSAIGVQCIGAKVN
                     HKLVPISHKLRSGDQVEIITSGKQRPTEDWLNIVVTAKAKSKIKDALKEEKRKIADEG
                     KYIVQRKLENFGAAYNQHNIDVLTAYYKLNSSLDFFYQVAVRTLDLKELQDFHLLGDR
                     LEPPKPMKLVEHKNADLPATPAVPRKDTELIIFGESSDRIVYTLANCCKPIPGDDVFG
                     FVSVGKGLTIHRTNCPNAA"
     gene            2121..2202
                     /locus_tag="VKU83_03310"
     tRNA            2121..2202
                     /locus_tag="VKU83_03310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2155..2157,aa:Leu,seq:tag)
     gene            2321..>2681
                     /locus_tag="VKU83_03315"
     CDS             2321..>2681
                     /locus_tag="VKU83_03315"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HLI92604.1"
                     /translation="MATVTRENIGLLTDKIVVNVSKGDYLPSFEKALKQYGKQANIPG
                     FRKGMVPPGLIRKMYGNSVFADEVLRTVEKELTTYMSTEKLEIFAQPLPLPENDSRRI
                     DVSNPADYSFAFEIGLKP"
BASE COUNT          538 a          738 c          739 g          666 t
ORIGIN      
        1 gcggcattgg ggcagttggt gcggtggatg gtaaggcctt tgcccacgga aacgaatccg
       61 aagacgtcgt cgccggggat cggtttgcaa caattggcca gggtatagac gatgcggtcg
      121 ctgctttcac cgaagatgat cagctccgtg tccttgcggg gaaccgccgg cgtcgcaggc
      181 agatccgcgt ttttatgctc caccagcttc atcggcttag gcggctcgag ccggtcaccc
      241 agcaaatgga aatcctgcag ctctttcagg tcaagggtgc gtaccgcaac ctggtaaaag
      301 aaatcgagtg aagagttgag cttgtaataa gccgtcagga catcgatgtt atgctggttg
      361 tatgcggcgc cgaaattttc cagcttccgc tggacgatat acttgccttc atcggcaatt
      421 tttcgctttt cttccttgag cgcgtccttg atcttgctct tggccttcgc cgtcacgacg
      481 atgttcagcc agtcttccgt cggccgctgc ttgccggatg tgatgatctc gacctggtcg
      541 ccgctgcgga gcttatgact gatcggtacg agcttatggt tgacctttgc gccgatgcac
      601 tgaacaccga tcgcgctgtg gatcgaaaaa gcgaagtcca gcgcagtgga gccgacgggc
      661 agcattttca catcgccgcg cggcgtgtac acgtagatct cctcggcgag gaagctggtt
      721 ttgaagtcct gcaaaaaatc gatggaatcg ttgtcttccg tgttgagcac ttcccggatt
      781 tgctggaacc atttgtcgaa ccggctttcg tcggccgctc cttccttgta cttccagtga
      841 gcggcaagcc ccttttcggc gatctcgttc atccggcggg agcggatttg cacttccacc
      901 cactttccct ggggacccat cacggtggtg tgcagggcct catatccatt ggacttggga
      961 ttgcttagcc aatcccgcag gcgctccggc gaggggttgt actcatccgt gatcatcgag
     1021 taaaccttcc agcagtcctc cttctccttc tccacggttg aattcaggat cacccggatc
     1081 gcaaacagat catagacctc ctcgaacgcg acgcccttct tgcgcatttt gttccagatg
     1141 gagtggatgc ttttcggccg gccgtaaatg tcgaagttga agcctgcctt ttccagcttg
     1201 tctttcaggg gcttgacgaa ttcattgata taccgcgacc tttccctttt cgtttccgcc
     1261 agtttgcggg cgatttcccg gtattcctcg ggttccaggt atttcatcgc caggtcttcg
     1321 agttcggtct tgatcgtata caaacccatc cggtgcgcca gcggtgcata gacatagacg
     1381 gtttcggagg cgatcttcag ctgtttttcc cgcttcatac tgtcgagcgt ccgcatattg
     1441 tggagccggt cggcaagttt gatcaggatg acacgcgggt cgtcggtgag cgtaaggagg
     1501 atcttcttga agttctcggc ctgccgcgat gcgttcacgt ccacaacgtt ggagatcttc
     1561 gtgaggccgt cgacgatccg ggcgatctcg ccgctgaact cccgttcaat gtcttccagg
     1621 gtgatatccg tgtcctccac cgtgtcgtgg agcagggcgc agatcgtgga gcgtacaccc
     1681 aggccgatct cttctgaaca gatcctggca acggcgagcg gatgcaggat atagggctcg
     1741 ccgctcttgc ggcgcatggt tttgtgcgcc tcggccgcca tttcgaaggc agaacggatc
     1801 agctccttat ccccaatttt gagtttcggt ttcagtgaac gaagcagggc ccggtattcg
     1861 cgcagaataa gccttttttc ctggtcttcc gtaagattgt atttcggtat tgctgctact
     1921 gtctccataa aatcaccggg gatggcaacc catcatttag cgcatccccg tgaaccgcta
     1981 aagattgtcc tgttgccggc gccttagccg cctcaactta cgaatttacg aaaaaaaacc
     2041 tatgtttgca ggctttccgt cggccgggaa cgcctgggcg atggcggtag tagtagctgg
     2101 cagacaataa attatccgat gcggatgtgg cgaaactggc agacgcacca gacttaggat
     2161 ctggcgccgc aaggcatgta ggttcgagtc ctatcatccg cacaggcctc ccggccctgg
     2221 ggcatttggc ggcccggggc caccgcccgg ggtattccga tcgcaacgga tttccgaaaa
     2281 atgcctacct ttgccccccg cttaaaaatc actcaaatct atggcaactg taacgaggga
     2341 aaacatcggc cttttgaccg ataagatcgt ggtcaatgtc tcgaaaggcg attatctgcc
     2401 gtcctttgaa aaggctttga aacaatatgg gaaacaggcg aatatccccg gcttcaggaa
     2461 aggaatggtg ccccccgggc tcatcaggaa gatgtatggc aattcggtct tcgccgacga
     2521 agtgctgcgg accgtggaaa aggagctgac gacgtatatg tccaccgaaa aactggagat
     2581 ctttgcgcaa ccgctgccac tgcccgaaaa cgattcccgc aggatcgatg tcagtaatcc
     2641 cgcagattat tcatttgctt tcgagatcgg gctcaagccg t
//
LOCUS       DATGKE010000189         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_681966, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000189 DATGKE010000000
VERSION     DATGKE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681966"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VKU83_03320"
     CDS             complement(<1..1489)
                     /locus_tag="VKU83_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92605.1"
                     /translation="MKFIIALCCLCPVLARATGYYISSSTGNNGNNGTSASTPLQTIA
                     FFNLHFSPKGGDTVYLKSGDTFNEALNLAFRGSPSPHLVYTTYGGSARAVISGMFKLT
                     GGTQIGSSNVWEFFCPNLNKNTNMLVMDGVPQPMGRWPDAGYRTFTYINTNKLYDMNL
                     MGAPNWTGAFVVAQTEHYIIDTALITNQSNDTIFADKNFSAFNTGRGNGYFIENDQRT
                     LQMTSVTGRWYNNYTKDSMQVYLPGGLGSHVLQVPTLDSLCYFNRHGYVDVYNLDFEG
                     SYNAAVLMNTDTALTMTNCLLRYGASNGISGNQSLQASFVNDTLDYFQNNGVEQTGSN
                     TAHGLFKNVLINHSGLIPGLGASSGGNGAASYTGCNWTFGTTTFQNTTILNSGYTGLC
                     YGGDSVNVTDCVVDTFCLVKIDGGGFYTTDLSFLSYTYGRHLTSCLALHGQNITSGVP
                     PDTTDASFGFYLDSHSQAVTLTGCTGAYNVSGGLFIHGSHDTSYAN"
     gene            1596..2603
                     /locus_tag="VKU83_03325"
     CDS             1596..2603
                     /locus_tag="VKU83_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92606.1"
                     /translation="MENSDPSSISPSAYTLLFMKRHTEIPYIREAAALVENKLRQAET
                     AGAGPTFIPQFSTDPAGYGIFLLHFEDRYWSIDQLMADLPITNMLELSSGFSFRGLDL
                     SRRKPVYYIDTDLPGLIAVKQSLVDDLLAGEQSAGGTPPDSSAADPTPGTPPGHYELQ
                     TLDVLDPDAFAAIVARFPPGPLVILNEGLLVYLDTTQKEQLCTNIRKALEARGGYWIT
                     ADIYIRNPADNPPAGQDPAANPNPAASPSPAASEQYTQWQAWKNQFQIEQKKFSGFAK
                     AQAFFTRMGYKIDKEATPDYPRLTSLPFLRTTAGPQFLEHLRQRGRLRLRTTWRLTLQ
                     S"
BASE COUNT          592 a          809 c          661 g          550 t
ORIGIN      
        1 tgttggcata gctggtgtcg tgggacccat ggataaagag tccgccgctg acattgtagg
       61 cgccggtgca tccggtaagc gtcacggcct gggaatggga atccaggtaa aaaccgaacg
      121 aggcgtcggt agtgtcaggc ggaacgcccg atgtaatatt ttgtccatgc agggccagac
      181 agctggtgag atgccggcca taggtatagg aaaggaagga caggtcggta gtatagaaac
      241 cgcccccatc gatcttcacc aaacaaaagg tgtcgaccac gcagtcggtg acattgacgc
      301 tgtcgccgcc atagcacaga cccgtatagc cgctgttcag gatcgtcgta ttttgaaagg
      361 tggtagtgcc aaaagtccaa ttgcagccgg tatacgaggc agcgccgtta ccgccgctgg
      421 acgcgcccag acccggtatc agtccgctat ggttgatcag tacattttta aatagcccgt
      481 gggccgtgtt cgagccggtc tgttccaccc cgttgttctg aaaataatcg agtgtatcgt
      541 tcacaaagct ggcctgcagg ctttggttgc ccgatatacc attggaagcg ccataacgta
      601 gcagacagtt ggtcatggtc agcgccgtat cggtgttcat caggacggcg gcgttatagc
      661 tgccttcgaa gtcgagattg tatacatcca catagccatg ccggttaaaa taacaaaggc
      721 tatccagcgt gggcacctgc aggacatgcg accctaaccc gcccggaagg taaacttgca
      781 tgctgtcctt ggtataatta ttataccagc gtcccgtgac ggacgtcatt tggagcgtcc
      841 gctggtcgtt ctcgataaag tacccgttcc cccggccggt attgaatgcg ctgaaatttt
      901 tatcggcaaa gatcgtatcg ttgctttgat tcgtaataag cgccgtatcg atgatgtagt
      961 gttcggtttg agccaccaca aaggcgccgg tccagttcgg cgcacccatc aggttcatgt
     1021 catagagctt attggtattg atgtacgtga aggtccggta cccggcatcc ggccagcggc
     1081 ccatgggttg cgggacgccg tccatcacga gcatattggt gttcttgttg aggttcggac
     1141 aaaaaaactc ccagacatta gagctgccga tctgcgtacc gcccgtcaac ttgaacatcc
     1201 cgctgatcac cgcccgcgcg gagccgccat aggtggtata gacgagatgc ggcgaggggg
     1261 atccccggaa agccaggttc aaggcctcgt tgaaggtgtc gcccgatttc aggtacaccg
     1321 tatccccgcc cttcggactg aaatggagat tgaaaaatgc gatcgtctgt aaaggcgtcg
     1381 aagcgcttgt cccattgtta ccgttgttac cggtggagga agaaatgtaa taacctgtcg
     1441 cccgcgccaa caccggacac aagcaacata gcgcaataat gaatttcata gtcaaggttt
     1501 ttataatgcg actaacgcgg atcccccgac tttattgaat tcctgcagcc acactgcgcc
     1561 gaacccgcgt caaaccattg cgctatcttt ggcggatgga aaatagcgac ccttcttcca
     1621 tcagcccttc cgcctatacg ctcctcttca tgaagcgcca cacggaaata ccctacatcc
     1681 gtgaagccgc cgccctggtc gaaaacaagc tgcgacaagc cgaaaccgcc ggcgccggcc
     1741 ctaccttcat tccccaattt tcaaccgacc ctgccggcta tggcattttc ctgttgcatt
     1801 tcgaggaccg ttactggagc atcgaccagc tgatggccga tttgcccatc accaatatgt
     1861 tggaactttc ttccggcttc tccttccgcg gtctggacct cagccgccgt aagccggtct
     1921 attatatcga tacggatctc cccggcctca tcgccgtcaa acaatccctc gttgacgacc
     1981 tcctcgccgg cgaacaaagc gccggtggca caccccccga cagttccgcc gcagatccaa
     2041 cccccggcac accccccggc cactacgagc tccaaaccct cgacgtcctg gacccggacg
     2101 ccttcgccgc catcgtcgcc cgcttccctc ccggcccgct cgtgatcctc aatgaaggcc
     2161 tcctcgtcta tctcgacacg acccaaaaag aacagctctg caccaatatc cgcaaagcgc
     2221 tcgaagcccg cggcggctac tggatcaccg ccgatatcta catccgcaac cccgccgaca
     2281 accctccggc cggccaggat ccggccgcaa accccaaccc cgcagcaagc ccaagccccg
     2341 cagcctccga gcagtatacc caatggcagg catggaagaa ccaattccag atcgaacaaa
     2401 agaaattctc cggcttcgcc aaagctcagg cgtttttcac ccgcatgggc tacaaaatag
     2461 ataaggaggc aacaccggat tatccccggc ttacctcctt accttttctt cggacgaccg
     2521 cgggcccaca gttcctggag cacctccggc agcgtggccg tctccgactc cgtactacct
     2581 ggcggttaac gctccagtcc tagctccggc at
//
LOCUS       DATGKE010000190         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_471868, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000190 DATGKE010000000
VERSION     DATGKE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471868"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VKU83_03330"
     CDS             complement(<1..891)
                     /locus_tag="VKU83_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HLI92607.1"
                     /translation="MKALVCIRPGSMEYREMTAPIAGPGQAIIRIRRIGICGTDLHAF
                     EGTQPYFSYPRILGHELAGDLVDVDGAPGFVPGEAVTIIPYFHCGTCIACRNGKPNCC
                     TMIEVCGVHIDGGMVDYLSVPSGSLVHANGLSYDQLALVEPLAIGAHAVRRAEVQSGE
                     FVLVVGAGPIGLGTMEAARIAGGEVIAMDINEDRLRFCHDALQVLHTVKGGPEAFETI
                     SDITGGDMPTVVVDATGSLQAINRAFEYMAAGGRYVLVGLQKGEIGFSHPEFHKREGT
                     LMSSRNATREDFDRVMSAMGR"
     gene            complement(1007..1795)
                     /locus_tag="VKU83_03335"
     CDS             complement(1007..1795)
                     /locus_tag="VKU83_03335"
                     /inference="COORDINATES: protein motif:HMM:NF006384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI92608.1"
                     /translation="MDLKLEGKVIVVTGGAKGIGEGIVRLLAREGAIPFIVGRNEGDN
                     QAAVAAAEAAGGKAFQVVAELTEPGASAAAVKKVMEVCGRIDGLVNNAGVNDGVGLEK
                     GSYDAFMASLHKNLVHYYLMAHHALPALKLSRGAIVNIGSKTAETGQGGTSAYAAANG
                     GRNALTREWAVELLKYGIRVNAIIVAECWTPLYERWIHTLPDPKGKLEEIVKKIPLGN
                     RMTTADEIANMTAFLLSERSSHTTGQLIHVDGGYVHLDRALANA"
     gene            complement(1805..2668)
                     /locus_tag="VKU83_03340"
     CDS             complement(1805..2668)
                     /locus_tag="VKU83_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI92609.1"
                     /translation="MAASDHLKAAPARIDAHQHFWKYDPMAHSWISDEMYAIRRDFAP
                     SDLQPLLAGAGFDGCVTVQVNQTEDENAVMLEQAAKHDFIKGIVGWVDLQDHRVEERL
                     AAYREYPLMKGFRHILQGERDRALMLKPAFKRGIASLTKYGFTYDILIYPDQLGYTRE
                     FVAAFPSQPFVIDHIAKPHIKDRYITEEWKEAIRAVAVYENVYCKISGLVTEADWGGW
                     KQEHLRPYMDTVVEAFGPARILFGSDWPVCLLAATYQQVFNIADEYFAAFSADERAAF
                     FGGNAVKFYHL"
     gene            complement(2655..>2868)
                     /locus_tag="VKU83_03345"
     CDS             complement(2655..>2868)
                     /locus_tag="VKU83_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLI92610.1"
                     /translation="ANLIFGIPYLVAYVSQFMTLLPGDVISTGTPAGVGLGMKPNVYL
                     KPGDVVELGIDHLGKARQNVKAYGSE"
BASE COUNT          576 a          964 c          716 g          612 t
ORIGIN      
        1 cctgcccatg gccgacatta cccggtcaaa gtcttcacgc gtcgcattcc ggctgctcat
       61 cagcgtgccc tcccttttgt ggaattccgg gtggctgaac ccgatctccc ccttctgcag
      121 gccgacgagc acatagcggc cgccggctgc catatattcg aaagcccgat tgatcgcctg
      181 gaggctccct gttgcatcga ctaccaccgt cggcatatcg cctccggtaa tgtcgcttat
      241 cgtttcaaat gcctccggtc cgccctttac cgtgtgcaag acctgcaacg cgtcgtggca
      301 aaaccgcagg cggtcttcat tgatgtccat cgcgatcact tccccgcccg caatccgcgc
      361 cgcctccatc gtgcccagcc cgatcgggcc cgcgcccaca accagtacaa attccccgga
      421 ttggacctcc gcccgccgga ccgcatgggc gccgatcgcc agcggctcta ccagtgcgag
      481 ctgatcatag cttagcccgt ttgcatgcac cagagatccc gacggcaccg acaaataatc
      541 caccatgccg ccgtcgatat gtaccccgca gacctcgatc attgtgcagc agttcggttt
      601 accgttacgg caggcaatgc aggtcccgca atgaaaatag gggattatcg tgaccgcctc
      661 cccgggcaca aagcccggcg caccgtcgac atccaccagg tccccggcca gctcgtgacc
      721 caggatgcgc ggataactga aataaggctg tgtaccttcg aaggcatgca agtcggtgcc
      781 gcaaatcccg atccgccgga tcctgataat cgcctgacca ggacctgcga tcggcgccgt
      841 catctcccgg tattccatcg accctggccg tatacaaact aatgccttca tatccgttta
      901 tcatgtgcgg gcggccgcga cgaatttctg gtcgcgacgc atttctggcc gcgacgcatt
      961 cgcggtcgcg acgctttccc ggccgcggga aacccgcggc cgctccttat gcattggcga
     1021 gcgccctgtc caggtgtaca tatcctccgt ccacgtggat caactggccc gtcgtatgac
     1081 tcgaccgctc ggaaaggagg aacgcggtca tgttggcgat ctcgtcggcg gtcgtcatcc
     1141 tgttcccgag agggatcttc ttcacgattt cttcgagctt gcctttcgga tcgggcagcg
     1201 tatggatcca gcgctcgtac aacggcgtcc agcattccgc cacgatgatc gcattcactc
     1261 ttatcccgta tttcagcaat tcaacggccc attcccgggt cagcgcattc cggcctccgt
     1321 tcgccgccgc gtaggccgac gtgccgccct gtcctgtctc ggcggttttt gatccgatgt
     1381 tcacgattgc gccccgggaa agcttcagcg ccggtaacgc atgatgcgcc atcagataat
     1441 aatggaccag attcttatgc aaggaggcca taaaggcatc atagctcccc ttctccagtc
     1501 cgaccccatc attgacgccg gcattgttta ccaggccgtc gatccggccg caaacctcca
     1561 tgactttttt gactgccgcc gccgacgcac cgggctcggt caactccgcc acaacctgga
     1621 aggccttgcc ccccgccgcc tctgccgcgg ccactgccgc ctggttgtcg ccttcgttac
     1681 gtccgacgat aaacggaatg gcgccttccc tggccagcag gcggacaatc ccttccccaa
     1741 tacccttcgc cccgccggtt acaacgatca ccttaccttc taatttaagg tccatataca
     1801 ggctttataa atgataaaac ttcaccgcat tgccgccgaa aaaagcagcc cgctcgtcgg
     1861 cagagaatgc ggcaaaatat tcgtccgcaa tattgaagac ctgctggtac gtcgccgcca
     1921 gcaggcagac cggccaatcc gacccaaaca ggatccttgc cggaccaaaa gcttctacca
     1981 ccgtatccat gtacggcctg agatgttcct gcttccagcc gccccaatcc gcttcggtca
     2041 ccaggccgga gatcttgcag taaacattct cataaacggc cacggcccgg atcgcttcct
     2101 tccactcctc cgtgatataa cggtccttga tatgcggctt ggcgatgtga tcgatcacga
     2161 aaggctggct gggaaacgcc gctacgaatt cccttgtata gccgagctga tccggataaa
     2221 taaggatatc ataggtaaat ccgtattttg tcaacgacgc gatgccccgc ttgaacgccg
     2281 gcttcagcat aagcgcccgg tccctttctc cctgcaggat atgccggaaa cccttcatca
     2341 gcggatactc gcggtacgct gccagccgtt cttccacgcg gtggtcctgc agatcgaccc
     2401 agccgacgat ccctttgatg aaatcatgct tcgcagcctg ctccagcatg acggcattct
     2461 cgtcttctgt ctggttgacc tgcacggtca cgcaaccatc gaatcccgca cccgccagga
     2521 gcggttgcag gtccgaagga gcgaagtccc ggcgaatggc atacatctca tcgctgatcc
     2581 aactatgcgc cattggatcg tatttccaaa aatgctggtg cgcatcgatc cgggccggcg
     2641 ccgctttcag gtgatcactc gctgccatat gcttttacat tttgacgggc cttgcccaaa
     2701 tgatcgatac ccagttcaac cacgtcgccc ggcttcaggt agacgttcgg cttcatcccc
     2761 agtccgacgc ccgcgggagt gccggtcgag atgacatccc ctggcaacaa cgtcatgaat
     2821 tgactgacat aggcaaccag gtacgggata ccaaagatca gattggct
//
LOCUS       DATGKE010000191         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_59585, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000191 DATGKE010000000
VERSION     DATGKE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59585"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2484)
                     /locus_tag="VKU83_03350"
     CDS             complement(<1..>2484)
                     /locus_tag="VKU83_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92611.1"
                     /translation="RFIRQYKHNPADSNSLGGDNVYAITDDGEGTVWVATYGEGLSQY
                     NSRTGGFHHFKHVASDPNTPGSNRLHTLMSDSNGILWIGTYDGGLDRLDAYTGIFTHY
                     RHEPGRNSLSNNTVNYIYADSRHQLWICTADGLNRLDRKTGHFTVYGEKDGLPSAVIF
                     GILEDNNGMLWVSTNDGLSRFDPGTGTFKNFSAADGLQSNQFKAHACFKSRAGDLYFG
                     GVNGFNRFNPDSIRDIPFDPPMLVTGFQIFNKDVPIGDSLQPSPLTADISMTRDITIA
                     HQNSVISFEFASLNYTVPEKKHYAYMLDGFDKGWNDVGLKRTVTYTNLDPGAYTFRVR
                     GWDNDGRWSNAVARIGLTVLPPYWQTWWFRLLATVLIVGGVVGVFRLRLHSVRMQKRK
                     LEAEVDHRTNLLALSRDEERRAREQAEKASRAKSEFMANISHELRTPMNAIIGFTDLV
                     LTTDLQRPQREYLENVHRSGYNLLGIINDILDYSKMEAGKLTIENTAFRLCQLVEQTV
                     DSLAIKAFEKNLELICQTDPSLPMQVLGDPGRIQQILVNLLGNGIKFTEAGEVVVALG
                     RGMVIQGPDGRRYQPVTLSVRDTGIGIPPEKLEHIFESFAQADSTTTRKYGGTGLGLT
                     IAKNLAHMMDGTLDVQSQMGKGSVFTLRLTFEIVDEMSSVPLVQRPVLKRALVVDDNI
                     TNCQLLENIFKYIGVDCQTCTSGPEALDLLGKVKVEEFFDLIVTDHQMPVMDGIRFVK
                     HVKALLKGRREPYILMLSSLDKGMCLEDAGQVGVDLFLSKPVKLRELNNMLQSIFGNT
                     RDSRSGDAGKPEIRKVAEGASILVAEDEPV"
BASE COUNT          498 a          735 c          675 g          576 t
ORIGIN      
        1 caccggctcg tcctccgcca cgaggatgct cgcgccctca gccaccttgc ggatctccgg
       61 cttgcccgca tccccggacc tgctgtcccg cgtgttccca aagatcgatt gcagcatatt
      121 attgagctcc cgcaacttca ccggctttga aaggaacaag tcgacgccga cctggccggc
      181 gtcttcgagg cacatgcctt tatccagcga agagagcata aggatatagg gctcccgccg
      241 gcccttcagc agggccttga catgcttgac gaaacggatg ccatccatta ccggcatctg
      301 gtgatccgtc acgatcaggt cgaagaattc ttcgaccttt accttgccga ggagatcaag
      361 ggcctcgggc ccgctcgtgc aggtctggca atcgacgccg atatatttga agatattttc
      421 cagcagctgg caattcgtaa tattgtcatc gacgacaagc gcccgcttga gcacaggacg
      481 ttgcacgagc gggacggaag acatctcgtc cacgatctcg aaggtcagcc tcagcgtgaa
      541 tacacttcct tttcccatct ggctctgcac gtcaagggtc ccgtccatca tgtgcgccag
      601 gtttttcgca atggtcagcc caagacccgt accgccatat ttccgcgtcg tcgtcgaatc
      661 ggcctgcgcg aagctttcaa atatatgctc cagtttctcg ggcggaatgc cgatgccggt
      721 gtccctgacc gaaagcgtga ccggttgata tcttctgccg tcggggccct gaatgaccat
      781 tccgcgcccg agtgccacga cgacctcccc ggcttccgtg aatttgatgc catttccgag
      841 caggttgaca aggatctgtt gaatgcgccc ggggtcaccc aatacttgca tcggcaagct
      901 gggatcggtc tggcagatca gttccagatt tttctcaaag gcttttatcg ccagactgtc
      961 taccgtctgc tccacgagct gacaaagcct gaaggccgta ttttcgatgg taagcttgcc
     1021 ggcctccatt tttgaatagt cgaggatatc gttgatgatg ccgagcaggt tataacctga
     1081 ccggtgcaca ttctccaggt attcccgttg cggccgctgc aagtccgtcg tcagcaccag
     1141 gtccgtaaag ccgatgattg cgttcatcgg cgttctcagt tcatggctga tattggccat
     1201 gaactcgctc ttcgcccggc tggccttttc cgcctgctct ctcgcgcgcc gctcctcgtc
     1261 ccgcgacaag gcgagcaggt tcgtccggtg atcgacctcc gcttccagct tccgcttttg
     1321 catccggacc gaatgtagcc gcagcctgaa aacgcccact acgccaccca cgatcagtac
     1381 ggtcgccagc aagcggaacc accaggtctg ccagtacggc ggcagcacgg tgagcccgat
     1441 acgcgcaaca gcattcgacc atctgccatc gttatcccag cccctcaccc ggaaagtgta
     1501 ggcgccgggg tcgaggttcg tataggttac cgtccgcttc agtcctacgt cgttccatcc
     1561 tttgtcgaag ccatccagca tataggcata atgttttttt tcaggaacgg tatagttcag
     1621 ggaagcgaac tcaaaggaaa tgaccgaatt ctgatgggcg atcgtgatgt cccttgtcat
     1681 ggagatatcg gccgtcagtg gcgatggttg cagggaatcg ccgatcggca cgtccttgtt
     1741 gaagatctgg aaaccagtca ccagcatcgg cgggtcaaag gggatatccc ggatgctgtc
     1801 ggggttgaac cggttgaatc cgttgacgcc accgaagtac aggtcgccgg cccggctttt
     1861 aaagcaagca tgcgctttga actggtttga ttgcaatccg tcggccgccg aaaaattctt
     1921 gaatgtaccg gtcccagggt cgaaccgcga aaggccatcg ttggtgctca cccataacat
     1981 gccgttattg tcttcgagga tgccgaagat gacggcgctt ggaagtccgt ctttctcgcc
     2041 atagaccgtg aagtgtccgg tcttccgatc gagccggttc agcccgtccg ctgtacaaat
     2101 ccacagctgg tggcggctgt cggcatagat atagttgacg gtgttattgc taaggctgtt
     2161 gcggcccggc tcgtggcggt agtgggtaaa aatgcccgtg taggcatcga gcctgtcgag
     2221 gccgccgtca taggtcccga tccagagaat gccattgctg tccgacatca gcgtatgaag
     2281 ccggttgctg cccggcgtat tcggatcgga cgccacatgc ttaaaatgat ggaacccgcc
     2341 ggtcctggaa ttatactggc tcaaaccttc tccatacgtg gcgacccaga cggtcccttc
     2401 cccgtcatcg gtgatcgcat aaacattatc gccgccgagg ctgttgctgt ccgcgggatt
     2461 atgcttgtat tggcggatga atct
//
LOCUS       DATGKE010000192         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1097008, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000192 DATGKE010000000
VERSION     DATGKE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097008"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VKU83_03355"
     CDS             <1..216
                     /locus_tag="VKU83_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HLI92612.1"
                     /translation="PQTGIDEDPVTGSAHTMTVPYWAERLGKTRLAAIQLSARRGYLD
                     CQLLGDRVLMTGEARTFLKGEILLPEN"
     gene            228..596
                     /locus_tag="VKU83_03360"
     CDS             228..596
                     /locus_tag="VKU83_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HLI92613.1"
                     /translation="MNENSAVAELSIREAQDLVDSWIRGTAAGYFDELTNMAVLTEEV
                     GEVARVISRTYGRQSFKESDRGRDLGDELADVLWVLLCIANQTGIDLTAALERNFQKK
                     NLRDRDRHTGKSERGSPPEV"
     gene            692..2143
                     /locus_tag="VKU83_03365"
     CDS             692..2143
                     /locus_tag="VKU83_03365"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HLI92614.1"
                     /translation="MANDSNRFQAIISHCKEYGFIFPSSEIYDGLSAVYDYGQYGSEL
                     KKNIKDHWWKSMTQLQENIVGIDAAIFMHPTTWKASGHVDGFSDPMIDNKDSKKRYRV
                     DHLIEARANELESQGRKSEADALLLAMDGMLAADDFAGLKGLIDEQKIKCNVSGTANW
                     TEIRQFNLMFSTQLGSVSEEASEIYLRPETAQGIFVNFLNVQKTGRMKVPFGIAQIGK
                     AFRNEIVARQFIFRMREFEQMEMQFFIRPGTEDEWYEHWKAARMRWHVGLGIPKEKFR
                     FHDHTKLAFYAKAACDIEYEFPIGFKEVEGIHSRTDHDLSRHQEFSRKKMQYFDPELN
                     ESYIPYVIETSAGLDRTILMVLSEAYTEEDLSTPEKKDSRVVLRFPPRLAPIKLAVLP
                     LTKKDGLPDLAREVMAACKPHFRCFYEEKDTIGKRYRRMDAIGTPFCVTIDYQTKEDQ
                     TVTIRYRDTMQQERVALGDVAALVGRQIAGADI"
     gene            2427..2606
                     /locus_tag="VKU83_03370"
     CDS             2427..2606
                     /locus_tag="VKU83_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92615.1"
                     /translation="MRKQQRIRDLESELMRSNEETLEAQRAYVMLESKLRDQQSIPVI
                     PMKINGKETKEKISK"
     gene            complement(2668..>3141)
                     /locus_tag="VKU83_03375"
     CDS             complement(2668..>3141)
                     /locus_tag="VKU83_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI92616.1"
                     /translation="LFNEVKVGSQLTLRGPQGVFTLPDNIERDLFLICTGTGIAPFRS
                     MAHYIMDHNIPHRDIYLIFGCRKFGDALYREEMTGLAEKIPHFHYIPTFSREPASPNG
                     SYRTGYVHPVYEELCCKKQPAYFFLCGWKNMIDEAKQRITTLGYERKSIHQELYG"
BASE COUNT          795 a          789 c          866 g          691 t
ORIGIN      
        1 ccgcagaccg ggatcgatga agatccggtc acggggtctg ctcacacgat gacggttccc
       61 tattgggcag aacgccttgg aaaaacccgc ctggccgcca tccagctgtc tgcacgacgc
      121 ggctacctgg attgtcagct gttgggcgac cgggtactca tgaccgggga ggcccgaaca
      181 tttttgaaag gtgaaatact tttacccgaa aattaacgat ctgcaagatg aacgaaaact
      241 cagccgttgc cgaactgtct atccgcgaag cgcaggatct tgtcgactca tggatccgcg
      301 gcaccgccgc cggctatttc gacgagctga ccaatatggc cgtcctgacc gaagaagtcg
      361 gagaggtggc ccgggtcatc agcaggacat atggacggca gtccttcaaa gagtcggacc
      421 ggggtcggga cctcggcgat gaactggcgg atgttctgtg ggtcctgctc tgcatcgcca
      481 atcagaccgg aattgacctg acggcggcgc tcgaaaggaa ttttcagaaa aagaatctcc
      541 gcgacagaga ccgccacacc ggaaagagcg aacgaggttc cccgccggag gtttgatgcc
      601 cgcttttgga tatcctgcct gtaacatatc tttgccccct taaacaccgg aatgcgcgac
      661 attggttaac agtgcagtcc ggtaaaaatt tatggcaaac gacagcaacc ggtttcaggc
      721 gatcatctcg cactgcaaag aatatggttt tatattcccg tcaagcgaga tctatgacgg
      781 gcttagcgcg gtttacgact atggccagta tggcagcgaa ttgaagaaga acatcaagga
      841 tcattggtgg aagagcatga cacagctgca ggagaatatc gtagggatcg acgccgccat
      901 cttcatgcat cccaccacct ggaaggccag cgggcatgtc gacggattca gcgacccaat
      961 gatcgataac aaggacagta aaaaacggta ccgggtagac catcttatcg aggcgagggc
     1021 gaatgaactg gagagccagg gacgaaagag cgaagccgac gcacttttgc tggcgatgga
     1081 cgggatgctt gcggccgatg actttgccgg cctgaaggga cttatcgacg agcagaagat
     1141 caaatgcaac gtaagcggca cggccaattg gacggagatc cggcagttca acctgatgtt
     1201 ctcgacgcaa ctgggttccg tgtccgaaga ggcgagcgag atctatctgc ggccggagac
     1261 cgcccagggg atcttcgtta atttcctcaa cgtccagaag acgggcagga tgaaggtccc
     1321 gttcggcatc gcgcagattg gaaaagcctt ccgcaacgag atcgtggcgc ggcaattcat
     1381 cttccggatg cgggaattcg aacaaatgga aatgcagttc tttatccggc ccggaaccga
     1441 agacgaatgg tatgagcatt ggaaagctgc ccggatgagg tggcacgtgg ggctgggcat
     1501 cccgaaagag aagtttcgct ttcatgatca tacgaagctg gctttctatg ctaaagcagc
     1561 atgcgatatc gagtatgaat tcccgatcgg attcaaggag gttgaaggca tccattcccg
     1621 caccgaccac gaccttagcc ggcaccagga attcagccgg aagaagatgc agtattttga
     1681 cccggagctg aatgaaagtt atattcctta tgtgatagag acttctgccg gcctcgaccg
     1741 gaccatcctg atggtgttga gcgaagcgta cacagaagag gatctcagca ctcccgaaaa
     1801 gaaagacagc cgcgtggtgc tccgcttccc gccgcgcctc gcgccgatca aacttgcggt
     1861 tcttccgctg acaaaaaaag acgggctgcc ggacctcgca cgcgaagtga tggccgcctg
     1921 caagccccat ttccgctgtt tctacgaaga aaaagacacg ataggcaaga ggtatcgccg
     1981 aatggatgcc ataggtaccc cgttctgtgt gactatcgac tatcaaacaa aagaggatca
     2041 gacggttaca attcgttacc gggataccat gcaacaggaa cgcgtggcgc tgggtgacgt
     2101 cgcggcatta gtcggccgcc aaattgccgg cgcggatatt taagaaaaaa aatagctgct
     2161 gcctgagatt tcggcacagt attgtgtgat ataatcgtta tatttgaatc ggatccgcag
     2221 ttccattctg ctccaactat cccctgaaag caatcggttg caactccctg ttgaagagtt
     2281 agatctcttc atttcgcgaa ctttttaata ctttatgtaa gatgggtgtt attttgacta
     2341 ctgattttgt cattaatccg gttattctca cggtggctgt gattttcggg ggtttatttg
     2401 gtttttttgt gttcaaggcg agtcttgtga gaaaacagca gaggatccgc gacctggagt
     2461 cggaactgat gcgaagcaat gaggaaaccc tggaagccca gcgtgcctat gttatgttgg
     2521 aaagcaagct gagggatcag caaagcatcc cggtcatccc gatgaagatc aacggcaagg
     2581 aaacgaagga aaagatttcc aaatagcctg cggttatctt tcaaccggca acagacgcgt
     2641 ttgtccgaca gggcaggcat gcaggcatta cccgtatagt tcctggtgaa tcgacttgcg
     2701 ctcgtaacct agcgtcgtga tccgttgctt ggcttcatcg atcatattct tccatccgca
     2761 tagaaagaag taggcgggct gttttttgca gcacaattcc tcatagaccg gatgcacata
     2821 tcccgtccgg tacgatccgt ttggactggc tggctcccgt gaaaaagtcg ggatgtaatg
     2881 gaaatgcgga atcttctccg ccaacccggt catttcttcc cggtacagcg catcgccgaa
     2941 tttcctgcag ccaaagatca ggtagatatc ccggtgcggg atattgtggt ccatgatgta
     3001 atgcgccatc gacctgaacg gagcaatgcc cgtcccggtg cagatcagaa agagatcccg
     3061 ttcgatattg tcgggaagcg taaagacacc ttgcgggccc cttagcgtta actgacttcc
     3121 caccttgact tcattgaata a
//
LOCUS       DATGKE010000193         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_371988, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000193 DATGKE010000000
VERSION     DATGKE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371988"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1714
                     /locus_tag="VKU83_03380"
     CDS             461..1714
                     /locus_tag="VKU83_03380"
                     /inference="COORDINATES: protein motif:HMM:NF028181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HLI92617.1"
                     /translation="MRYYFLFSSLLAAMSVFAQQPVDTIPFGGNAWQHSNEATIWFRI
                     SHPGPLQVQLLASGSRDTSIDLDITIGSPDRRPAISSLTHRLRVSGDQLHPYAAGSWT
                     IHDTGYTSVTIRQRRKSTLHLQSLILSGAAAQQTAYVPNDDGNFFYWGRRGPSVHLNY
                     AVPDALKVEWFYNEVTVPEGEDRIGSFFMADGFSVGYFGMQVNSASERRILFSVWSPF
                     NTDDPQAIPDSLRIGLVRKGDGVHAGTFGAEGAGGQSYLVYPWTAGHTYRFLLRAEPY
                     KSDPPNHTRFTAWFYAPETGNWRLIADWDRPATNTWLTHLHSFLENFEPEAGDRDRLA
                     LYANQWVADSTSHWYPLTRARFTGDNTARKGYRLDYAGGSRDSTFYLRNGGFFNQYTP
                     LDSWFDRPRPRQTAPPVDIASPFFR"
     gene            1726..>2030
                     /locus_tag="VKU83_03385"
     CDS             1726..>2030
                     /locus_tag="VKU83_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI92618.1"
                     /translation="MWKTSLPFFFALAIVCGASGQQTRTDTVVLHFTFDHSTVRPIDS
                     GLLLPVAGRPLDSILIAAYTDTTGSERYNQKLSLRRALAVQTALQHFLAMTPPQQHSL
                     "
BASE COUNT          391 a          703 c          480 g          456 t
ORIGIN      
        1 caccccgccg ctcatctact acaaagcgga tcccctcggg agatccactc cacccgttat
       61 gcagcggacg tactggataa acgaccccaa aacattggcg gattccgttg ccagcatcct
      121 gcatattaag ctataaacga cagcttttta taaaggtccg gcggcacacg gttgccgccg
      181 ggcggccgct acccggtgcg cggccgcagg ctggttatcc acttgttaac cccctccgcc
      241 ggcgttaaac taccccttcc ccaagccctc ttacttatga aacttaaaac tacgatcatg
      301 aaaaagttaa ttcatctcct tatcatcggc gccatcatcg ccatcggctt cagctcttgc
      361 gtcgtatacg ctcaccccgc acatccacat tattattatc accactggta gtatcgaggg
      421 acggttccgc cgtccctttt tattgtattt tgcatctcgc atgagatact atttcctgtt
      481 cagttctctt ttggccgcca tgtccgtctt cgctcagcag cccgtcgaca ccatcccctt
      541 cggcggtaac gcctggcagc attcaaatga agcgaccatc tggttccgca tctcccatcc
      601 gggtccgctg caggtacagc tcctggcctc aggcagccgc gataccagca tcgatctcga
      661 catcaccatt ggcagccctg accgccgtcc cgcaataagc agcctcaccc atcgactccg
      721 cgtctccggc gatcagcttc acccttatgc cgccggcagc tggacgatcc acgataccgg
      781 ctacacctcc gtcaccatcc ggcagcggcg caagagcacc ctccatcttc aaagtctcat
      841 cctatccggc gcggccgcac aacaaacggc ctatgtgccg aatgacgatg gtaatttctt
      901 ttactggggc cgccgcggcc cctccgtcca tctcaattat gccgtccccg acgcccttaa
      961 ggtcgaatgg ttctacaacg aagtaaccgt ccccgaaggg gaggacagga tcggctcttt
     1021 ctttatggcc gatgggttca gtgtcggcta cttcggcatg caggtcaact ccgcttcgga
     1081 acggcgtatc ctgttctccg tctggagccc tttcaacacg gatgacccgc aagccatccc
     1141 cgacagcctg cgcatcggtt tggtgcgcaa aggcgacggc gtccacgcgg gtacgtttgg
     1201 cgcggaaggg gcgggcggac aaagctattt ggtctatccc tggaccgccg gccatactta
     1261 ccgctttctt ctccgtgccg aaccgtacaa aagtgatccc cccaaccaca cccgcttcac
     1321 ggcctggttc tatgccccgg aaacagggaa ttggcgcctc atcgccgact gggaccggcc
     1381 cgccaccaat acctggctca cccaccttca tagtttcctc gagaacttcg aaccggaggc
     1441 cggcgaccgg gaccgcctgg ccctctatgc caatcaatgg gtcgccgatt ccaccagcca
     1501 ttggtacccg ctcacgcgag cgcggttcac cggcgacaac actgcccgca agggctatcg
     1561 tctcgattat gccggcggct cgcgggacag caccttctat ctgcgcaatg gcggcttctt
     1621 taaccaatat acgcccctgg attcctggtt cgaccggccc cggccacgcc agaccgcgcc
     1681 gccggtagat atcgcttccc cgttttttcg ctaaattgta ggtctatgtg gaagactagc
     1741 ttgccttttt tctttgcgct tgctatagtg tgtggtgcgt ccggccaaca gacccggacc
     1801 gataccgtgg ttttgcattt taccttcgat cacagtactg ttcgccccat cgattccggc
     1861 ctcctgctgc ctgtggcggg ccgccccctc gattctatcc tgatcgccgc ctataccgat
     1921 accaccggaa gtgaacgcta caaccaaaaa ctttcgcttc gccgcgccct tgccgtgcag
     1981 acagccctcc aacatttttt ggcgatgacg ccgccgcagc agcattccct
//
LOCUS       DATGKE010000194         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1001835, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000194 DATGKE010000000
VERSION     DATGKE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001835"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VKU83_03390"
     CDS             complement(<1..827)
                     /locus_tag="VKU83_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLI92619.1"
                     /translation="MYRRVIFFTTLFLVFQSAFSQSRKYVIAVIGSSTAQGVGADPID
                     SSWVNRTKSYYQSLGLIDTIYNIALGGQTTYGGMPSGFTPPAGRPAPDPATNVTKALS
                     FHPDVVLVNFPSNDAAADYSLTETMSNLRAIYGAVVAAGKIAFITTSEPRTSLETSQK
                     ELLKTMRDSVEAEFGANSLDFYDAIVGPDSLDINPAYNFDGTHVNNAGHRQLFEVVRN
                     AHILSGLAPAAPTLLGFTAKPEGNIVRLDWALTGASGPVTVLVQRSIDSTNFADIGR"
     gene            944..1384
                     /locus_tag="VKU83_03395"
     CDS             944..1384
                     /locus_tag="VKU83_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLI92620.1"
                     /translation="MPNNHFKKGELYSFITGKASTAIARNLQKKFGAAGLKITIEQWS
                     VLYQLWKEDGKSQQELCVATFRDKPSITRLVDNLEKQQLVKRVPAENDRRINLIYLTK
                     QALRLQEETMALAESTLNEALEGVAPEKIDICKEVLQVVYDNLR"
     gene            1417..>2323
                     /locus_tag="VKU83_03400"
     CDS             1417..>2323
                     /locus_tag="VKU83_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLI92621.1"
                     /translation="MSASTEKAQVLKGGEWLVKESSPFDCFIPEDYNEEQQMVKDMCS
                     AFLDTEVLPIVDRIDKLEPGLMPSLIKKAGDQGLLAAAIPEEYGGLGKDFITATLVNE
                     GLGGGFSFSVGVSAHTGIGTLPILYFGTPEQKQKYIPKLATGEWKGSYGLTEPNSGSD
                     ALGARTTAKLSADGKYYILNGQKCWITNGGFADIYTVFAKIDGDKFSAFIVERGFEGF
                     TQGPEEHKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHVIAFNILNIGRLKLCAAAL
                     GGAKRAATTSIGYARTREQFKTPIAH"
BASE COUNT          576 a          560 c          706 g          481 t
ORIGIN      
        1 tgccgcccaa tatccgcaaa gttcgtactg tcgatgctgc gttggaccag aacggttact
       61 gggccggatg cacccgtcag cgcccaatcg aggcggacga tgttcccttc cggtttggcg
      121 gtaaagccca ggagggtcgg cgccgccgga gcgaggcctg acaaaatatg ggcattccgg
      181 acgacctcga agagctgccg gtggccggcg ttgttgacat gggtgccgtc gaaattatag
      241 gcaggattga tgtcgaggga gtccggtccg acgatcgcgt cgtagaagtc aaggctgttt
      301 gcgccgaatt ctgcttcgac cgagtcgcgc atcgtcttca gcaattcctt ttgggacgtt
      361 tccagggaag tcctgggttc gctggtggta atgaacgcga tcttgccggc ggcgaccaca
      421 gccccgtaaa ttgcccgcag attggacatg gtttcggtaa gactgtaatc ggcggccgcg
      481 tcgttgctgg gaaaattgac gagtacgaca tccggatgga aggaaagcgc tttggttacg
      541 ttggtggcgg gatcaggcgc gggccggccc gcggggggcg taaaaccgga cggcatgccg
      601 ccataggtcg tttggccgcc gagtgcgata ttgtagatcg tatcgatcaa acccaggctc
      661 tgataatagg acttagtccg gtttacccag gaactgtcga tgggatccgc tccgaccccc
      721 tgtgcagtcg aagagccgat gacggcaatc acgtattttc tggattggga gaatgcggac
      781 tggaaaacaa gaaaaagggt tgtaaaaaag atcacgcggc ggtacatggc ggcagtttta
      841 attccgtagg ggattaggat cggcggggta taaagttagg ccaatttttt ttggctaatc
      901 cggaaaaatt gttgtagatt tatttagttg tctagctaac tgtatgccaa acaaccattt
      961 taaaaaaggc gaactgtaca gctttatcac cggtaaggcg agtaccgcta ttgcccgtaa
     1021 tctccaaaaa aagtttggcg ccgccggcct gaagatcacc atagaacaat ggagtgtcct
     1081 gtatcaattg tggaaggaag acggaaagag ccagcaggaa ctttgcgttg ccaccttcag
     1141 ggataaaccg agtatcaccc ggctggtgga caacctggaa aagcagcagc tcgtcaaaag
     1201 ggtgccggcg gagaatgacc gccggatcaa cctgatctat ctgacaaaac aggcgctgcg
     1261 attacaagag gagacgatgg cccttgccga atcgacgctg aatgaggcgc tggaaggggt
     1321 cgcgccggag aagatcgaca tctgcaagga ggtattacag gtcgtttatg acaatctgag
     1381 gtagagaaag attcaatcaa cataaaacga ttatttatga gcgcatctac tgaaaaagca
     1441 caagtcctga agggcgggga atggctggtg aaggaaagct ctccgttcga ttgttttatt
     1501 cccgaagact ataacgaaga gcagcagatg gtaaaagata tgtgcagcgc cttcctcgac
     1561 acggaagtgt tgccgatcgt cgaccggatc gacaagctgg agccgggact gatgccttcg
     1621 ctgataaaaa aggcggggga ccagggactg ctcgccgcag cgatcccgga agaatacggg
     1681 ggcctgggaa aagacttcat caccgcgacc ctggtgaatg aaggtctggg tggcggcttt
     1741 tcgttttcgg tcggcgtctc cgcccatacc ggtatcggca ccctgccgat attgtacttc
     1801 ggcacgcctg aacagaaaca gaagtatatc cccaagctgg caacgggcga atggaagggt
     1861 tcatacggct tgacggaacc gaacagcggc agtgatgcgc taggggccag gacgacggca
     1921 aagctttcgg cggacggcaa gtactatatc ctcaatgggc aaaaatgctg gatcaccaac
     1981 ggcggctttg ccgacattta tacggtcttt gcaaagatcg acggggataa gttctccgca
     2041 tttatcgtcg agcgcgggtt cgaaggcttt acacagggcc ccgaagagca taaaatgggg
     2101 atcaaggggt cctcgaccgt gcaattgtac ttccaggatt gcaaagtgcc ggtagagaac
     2161 ttgcttggcg agatcggcaa gggacatgtc atcgcgttca atatcctgaa catcggccgg
     2221 ctcaaattgt gcgcggccgc gttgggcggc gccaaacgcg cagcgacgac ctcgatcggg
     2281 tacgcgcgga cccgcgaaca attcaaaacg ccgatcgctc att
//
LOCUS       DATGKE010000195         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1509334, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000195 DATGKE010000000
VERSION     DATGKE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509334"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..345
                     /locus_tag="VKU83_03405"
     CDS             61..345
                     /locus_tag="VKU83_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92622.1"
                     /translation="MKKIKWTIMTLAVIVSISAAVGFRPKPVLSGLYYYNGTAYLPAG
                     TEGVNYVCLSSTDVCTYILQNGVYTPYETASSYQPINLAPPAEPSARKAK"
     gene            complement(381..1775)
                     /locus_tag="VKU83_03410"
     CDS             complement(381..1775)
                     /locus_tag="VKU83_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI92623.1"
                     /translation="MNPNPFIRLTIAIFLAIAVAAGGCRKAFLAQRPSSNLLIPNSLS
                     VLQELLDNERVMNVSPALGEVSADNYFLTSNTWTSLDTKDYNAYVWAADLYQGQGSVP
                     DWDLPYQQVFYANTVLQGLAQIRPDSTEVAEWNKLEGWALFSRAFAFFNIAELFAKPY
                     DSATAGMDPGIPIRLSPDINEKTTRATMQSTYDQILDDLHRAAAFLPVTVPGNNLNRP
                     SRLAAQALLARIYLSMRVYDSAGWYANTVLQAYPFLLDYNSLNVGGFVPFADAMREVL
                     FQANFPVVGNFLEGIVCRGCVVDTSLVDSYDSNDLRRPAFYFQRAADTFNMKGSYAGS
                     VFPFSGLATDEMYLIRAESEARAGNAAGAINDVNTLLAHRWRTGSFTAETSMTAPQAL
                     DTVLAERRRELAFRGIRWSDLRRLNQEGRLDTLHRVINGQVYTLFPNSDLYTLPIPPD
                     VIRFSGIMQNPREP"
     gene            complement(1787..>2438)
                     /locus_tag="VKU83_03415"
     CDS             complement(1787..>2438)
                     /locus_tag="VKU83_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92624.1"
                     /translation="SVYALRWKGLDSAGNPVGYLNATGQVSGQNTENYSAILNSPDLT
                     NLIYKGPMTPAFFGGWRNNFSWREWSLSINILYKMGYVFRRPSINYLALFNGTSPGNK
                     DFDRRWQHPGDELHTSVPSLPVPNVTANQPRDAFYQNSEVLIEKGDLIRLQDIQLTYD
                     ITKAPHPQLPLQSIRLYLYANNLGIIWKANRAGIDPDFISTFPNPRTLAIGIRAGF"
BASE COUNT          578 a          679 c          656 g          525 t
ORIGIN      
        1 gaggctccag caccggaagc ttttcaaatc catatatatc accaacaaac aaacattcgt
       61 atgaaaaaaa tcaagtggac cattatgacc ttagctgtca tcgtcagcat cagcgccgcc
      121 gtcggattcc ggccaaaacc cgttttatcg ggcttgtact attataacgg tacggcgtat
      181 ttgcccgccg gaacggaggg tgttaactac gtgtgcctgt cctcaaccga cgtttgcaca
      241 tacattctcc agaacggggt ttatacgcct tacgagaccg cctcgtcgta tcaacccatt
      301 aacctggcgc ctccggccga accttcggcg cgtaaggcaa aatgaccgcg ttcaaggaag
      361 tgtttttccg accccgggag tcaaggctcc cgggggtttt gcataatgcc cgaaaaccgg
      421 atgacgtccg ggggaatcgg cagcgtgtag agatcgctat tgggaaacag cgtgtatact
      481 tgcccattga tcacccggtg caacgtgtca agccggccct cctggttaag ccttcggaga
      541 tcagaccacc ggattccacg gaaggccagc tcccttcgcc gctccgctaa gaccgtatcc
      601 agcgcctggg gcgctgtcat tgacgtttcc gcggtaaaac tgccggttcg ccagcgatgc
      661 gcgagcaggg tattgacatc gttgatcgcc ccggccgcat tcccggccct ggcctccgat
      721 tccgcccgga tcaaatacat ttcgtccgtc gcaagtccgc tgaacggaaa aacggatccc
      781 gcataactgc ccttcatatt gaacgtatcc gctgcccgct gaaaataaaa ggccggtcgc
      841 cggagatcat tgctgtcgta agaatccacc aagctcgtgt cgacgacgca gccacgacag
      901 acaatccctt ccaagaaatt cccgacgacg gggaaattag cctggaaaag aacttcacgc
      961 atcgcatcgg caaatggcac gaagccgcct acgttcagcg aattgtaatc gagaaggaac
     1021 gggtaggcct gcagaacggt gttcgcgtac cagcccgcac tgtcataaac ccgcatgctg
     1081 agataaatgc gcgccaacag ggcctgcgcg gcgaggcggc tcgggcggtt gaggttgttc
     1141 ccggggacgg ttaccggcaa aaatgcggca gcgcgatgca ggtcgtccag gatctgatca
     1201 taagtggatt gcatggtcgc cctcgtagtc ttttcattga tatccggact caaccggatg
     1261 ggaattccgg gatccatacc ggccgttgca ctgtcatatg gcttcgcaaa cagttctgcg
     1321 atattgaaaa aggcaaacgc ccggctgaat aacgcccagc cttccagctt gttccattcc
     1381 gccacttcgg tggaatccgg ccggatctgg gccaggccct gcaacaccgt attggcataa
     1441 aaaacctgtt ggtaaggaag gtcccagtcg gggaccgacc cctgtccctg atagaggtcc
     1501 gcggcccaga cataggcatt atagtccttc gtgtccaggg atgtccaggt attggatgta
     1561 aggaaataat tgtccgcgga aacctcgccg agcgcaggcg aaacgttcat cacccgctcg
     1621 ttgtccagca gctcctgtaa gacactgagg gaattcggga tcagaagatt gctgctgggt
     1681 ctctgggcca gaaaagcctt gcgacagccg ccggccgcga cagcaatcgc caggaagatc
     1741 gcaatagtta gccggataaa aggattcggg ttcatgatat ccacttttaa aagccggctc
     1801 taatgccgat cgccagcgtt ctggggttgg gaaaggtaga aataaaatcg gggtcgatgc
     1861 ctgccctgtt ggccttccaa attatcccca ggttattcgc atacaaatac aatcggatcg
     1921 attgcagggg cagctgcggg tgcggggcct tcgtgatgtc ataagttagt tggatatcct
     1981 gcaggcggat caggtctccc ttctcgatca gcacctcgga gttttgataa aacgcatccc
     2041 tgggctgatt cgcggtcaca ttggggacgg gaagcgacgg cacactggta tgtaattcgt
     2101 ctcccggatg ctgccagcgc cggtcgaagt ccttgttgcc cggagaagtg ccattaaata
     2161 aagccaggta attgatcgat ggccgccgga agacgtaacc cattttgtat agaatattga
     2221 tcgagaggct ccattcacgc caggaaaagt tgttccgcca accaccaaaa aaagccggcg
     2281 tcattggacc cttgtagatt agatttgtca ggtcaggcga gttcagaatt gcactataat
     2341 tctcggtgtt ctgccccgac acctggccgg ttgcgttcag atagcctacc ggatttccgg
     2401 cagagtcaag tcccttccac cgcaatgcat acaccgaa
//
LOCUS       DATGKE010000196         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_804589, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000196 DATGKE010000000
VERSION     DATGKE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804589"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..438)
                     /locus_tag="VKU83_03420"
     CDS             complement(127..438)
                     /locus_tag="VKU83_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI92625.1"
                     /translation="MQSEPFYALAKKLGSLFDNSYDFLGRSDISLGYDAASKGYRIMR
                     TGTKRAANNLSEGEKTADAFVYFTIKMNENGNRIEDSIVLAGDPTLSSDSNHLFHFLL
                     F"
     gene            630..1604
                     /locus_tag="VKU83_03425"
     CDS             630..1604
                     /locus_tag="VKU83_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92626.1"
                     /translation="MPNNLWLAILSSLIVLVPAREREDSSLISTLKDVKKAYSVLHVI
                     DDQEEQLHLYVADKDQEEIRPFLGQCIKTCESLGGQTYSNSTLSTHMQNYLDQTIKNY
                     QTLNKLQPGQQGFIKKFDAHNTQRRNIADFLVTTFDLSHFVHLTEETYWKTVDKKNYI
                     RSARYSAYVELKKHNIPQALSLLDSIYRVTPDFQEKTIYQLELADEYEKNRNTLSNGT
                     NLAIKTYQSILDKKQYCLYLYEAWLKWRVVSQQYSGLSKSSDIPNNHYDEVRDQVANV
                     ILAHIQQNEKDEMAINQFFVITTHDIIFRFGEYPYGNQNTVEYHEIFQ"
     gene            1969..3516
                     /locus_tag="VKU83_03430"
     CDS             1969..3516
                     /locus_tag="VKU83_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLI92627.1"
                     /translation="MATPSEKLADSLDRLKELQDKGIVAIKSDALSRIHRERLVENGF
                     LEEVHRGWYIVVSPEAIKGDSTAWYSNYWDFCVQFLNERLGQNWCISPEQSLLLHSGN
                     TTVPSQMIIRSPENNNQRIELPYNTSLFLMRSSLPPENAHIEKDGIQMFSLPAALIGA
                     APSMFAKQPIDVRTALSLVSDASDILALLLDGGHSLIAGRIAGAFRNNNQERIADSIT
                     RTMQRAGYDVREVDPFQTKLVIPISGRVHSPYGNRIRLMWQMMREKIIPHFPTAPGLP
                     ANKKMYLDEVEKIYVTDAYHSLSIEKYKVTPELIERVRKGTWNAKGNEEDRKQLDAMA
                     ARGYWDAFQEVEKSIGRILDGKNSGEVAYSDHGEWYAALFGPSVTAGLLKPSDLAGYR
                     THQVYIVTSMHTPLNKEAVRDAMPVLFELLTEENEASVRAVLGHFVFVFIHPYMDGNG
                     RMGRFLMNVMLASGGYPWTVIPVQRRDEYMQALEKASVGQDIEPFARFLGYLTNEGVK
                     GTPVAKI"
     gene            3977..4315
                     /locus_tag="VKU83_03435"
     CDS             3977..4315
                     /locus_tag="VKU83_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLI92628.1"
                     /translation="MHELSVFMSGKKTALLPGPEKILTELGVNLKLARKRRKLTRNLV
                     AERAGIARSTLQLIEDGNPGVAMSSYLQVLFVLGLEKDLLRVAADDLLGRKLQDAALL
                     NKKKGNDKAN"
     gene            4299..5561
                     /locus_tag="VKU83_03440"
     CDS             4299..5561
                     /locus_tag="VKU83_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HLI92629.1"
                     /translation="MTKQIEVYADWIGLNGPEHIGRLTAQSIRGREIFSFSYRPEWLE
                     VAGALVLDPDLQLFTGAQYVRDEKPNFGLFTDSAPDRWGRVLMQRREALLAKKEERRP
                     RTLMESDYLLGVFDAYRLGALRFKLDKDGPFLDDQATMAAPPMTALRTLEEASLHLEQ
                     ENAADDPAFGVWLNQLLSPGASLGGARPKASVIDPEGRLWIAKFPSGGDDRDIGGWEA
                     VVNELALRAGLNVAQGQAKRLTLQHHTFLTRRFDRDGNWRIHFASAMTLLGRTDGADA
                     ATSSYLELAECIARVGARTNSDLEELWRRIVFNICISNADDHLRNHGFLLTSKGWVLS
                     PAYDLNPVPDAQGLTLNIDGTNNALDMELAGSVAPQFRVKSERSKQIIQEVTKAVNGW
                     SALAAKMGIPRAERERVGVAFDKVSNRS"
BASE COUNT         1782 a         1392 c         1577 g         1629 t
ORIGIN      
        1 taaacgcaag atctgatctt atccttctta taatcatttt ttttcatgat ccaatcccga
       61 ataagcttaa agtacagaag ttatgcgtga ggatgaaaag ctgcttcgcc gttgcgcagg
      121 tggctttcaa aaaagcaaga aatgaaaaag atgattggaa tcagaactca aggtggggtc
      181 gccggctaag acgatcgagt cctcgatcct gtttccattt tcattcatct taatggtgaa
      241 atacacgaag gcgtcggccg ttttttcgcc ttcgctcaaa ttattcgcgg cacgtttcgt
      301 tcccgtgcgc atgattctgt agcctttgga ggccgcgtca tatcccagcg agatatcgga
      361 tctcccgaga aagtcatagg agttatcgaa gagactaccc agttttttcg cgagtgcgta
      421 aaaaggctca ctttgcagtg aacctcgctg tagcgggcct tacgatacaa ttcttgaacc
      481 tttttatgtt gggcttgcgc gcccttgcaa atttgggtac tcgaattcaa tgatttatgt
      541 ctttgaaata tttggcttta ttggaaggag cgacttcagt caccgaccgg cttgcacttt
      601 ttttatactt ttacggacaa cctataccca tgcccaacaa cctctggctc gccattctaa
      661 gctctcttat tgtgttggtt cccgctcgcg aaagggagga ttcaagcctt atcagcacgt
      721 taaaagacgt taagaaggcg tacagtgttc tccacgtcat tgatgatcag gaagagcagc
      781 tccatctata tgtagcggat aaagatcagg aagaaattcg tccatttctc ggccaatgca
      841 tcaagacttg cgaaagcctt ggggggcaaa cttatagtaa cagtacgctt agcacgcata
      901 tgcaaaacta tctggaccag accatcaaaa attatcaaac actcaacaaa ctgcagccag
      961 ggcagcaagg attcataaag aaattcgatg cccacaatac ccagcggcgc aatatcgcgg
     1021 acttcctcgt aacaactttt gacctcagcc actttgtcca cctgaccgaa gagacctatt
     1081 ggaaaaccgt cgacaagaaa aactatatca ggtcagcgcg ctacagcgct tatgtggaac
     1141 tcaaaaaaca caacataccg caagcgctca gtttactgga cagtatttac cgggtgaccc
     1201 cggacttcca ggagaaaacc atttaccagt tagaattggc agatgaatac gaaaagaacc
     1261 gcaacacgtt gagtaatgga acgaatctgg caattaagac ataccaatct attctcgata
     1321 aaaagcaata ttgcttatac ttatatgagg catggctcaa atggcgggtg gtaagccagc
     1381 aatattcggg actttctaaa tcttctgata tacctaataa tcactatgac gaagtccgcg
     1441 atcaggtcgc caacgtcatc ctggcgcaca tccaacagaa tgaaaaagat gaaatggcta
     1501 tcaaccagtt tttcgtaata acgacgcatg atatcatctt ccgattcggg gaatatcctt
     1561 acggcaatca gaacacagtc gaatatcatg agatatttca ataaacaaga gggagggaac
     1621 agcaccctcc ggaaataaaa aatgccccgc gaatgcgggg ctacggattt tcgtgcggac
     1681 tggacgggac cgtcgaggcc aggacctaaa ttgtccggaa atttataaaa aacctgacaa
     1741 ctttgctagc aaacggattt tgctagtatt ttaaatggtt gacaagcaat gtattactag
     1801 attttaaatg gcggtagaat tttaaatgca aagcacagca ttattttagt gtatttgcac
     1861 agcatcattt tagtataaat taacggcatt actttagtat atttacacat cgttatttca
     1921 gtataaacac acggcatcat tttagtataa ccacacagga caagttatat ggcaacgcct
     1981 tccgagaaac tcgccgattc gttagaccgg ctaaaggaac tacaagataa aggaatagta
     2041 gccataaaat cggatgcact aagccggatc catcgcgaaa gattggtgga aaatggcttc
     2101 ctggaagaag tccatagagg gtggtatatt gtcgtttcac ctgaggcaat aaaaggcgat
     2161 agcactgcgt ggtacagtaa ctattgggat ttctgcgttc agtttctaaa tgagcggcta
     2221 ggacagaatt ggtgtatttc tccggaacaa tcgttgttac tgcatagtgg taacacaacc
     2281 gtaccctctc aaatgatcat tcggtccccg gaaaataaca accaacgtat tgagttgcca
     2341 tataatactt cattattttt aatgcggtcc agtttacctc ctgaaaatgc acacatcgaa
     2401 aaggacggca ttcaaatgtt ctctctgcct gcagcgctta taggggcagc tccgtcaatg
     2461 tttgccaaac agccgattga cgtacgaact gctctttctt tggtaagtga tgcctcagat
     2521 attctggcct tattattaga tggcggacat agccttatcg ccggaagaat agcgggcgct
     2581 ttccggaata ataatcaaga gcggatagca gatagtatca caagaaccat gcagagagct
     2641 ggctatgacg taagagaagt tgatccattt cagactaagt tggttattcc aatatcaggt
     2701 cgggtgcatt ctccttacgg caaccgaata aggttaatgt ggcagatgat gcgtgaaaag
     2761 attatcccac attttccaac agcccccggc ttgcccgcaa ataaaaaaat gtatttggat
     2821 gaggttgaga agatttatgt cactgatgcc tatcattccc tatccataga aaagtacaaa
     2881 gtaacacctg aacttattga aagggtaagg aagggtacat ggaatgccaa aggaaatgaa
     2941 gaagatcgaa aacaacttga tgctatggct gccagaggat attgggatgc cttccaggaa
     3001 gttgaaaaaa gcatcggtcg tattctggat ggaaaaaact caggtgaggt cgcatattcg
     3061 gatcacggag aatggtatgc ggctcttttc gggcctagtg taacagcagg tctcctaaag
     3121 ccatcggatc tggcgggata tcggactcac caggtatata ttgtcacctc gatgcatact
     3181 ccgctgaaca aagaagcagt cagggatgcc atgccggttc tttttgagct attaacagaa
     3241 gaaaatgagg cttctgtgag ggctgtctta ggacatttcg tgtttgtctt cattcatccc
     3301 tatatggatg gtaatggccg aatgggtcga ttcttgatga atgtcatgct agcctcggga
     3361 gggtacccgt ggacggtaat tccagttcaa cggcgagatg aatatatgca ggctctggaa
     3421 aaagcgagcg ttggccagga tatcgagcct tttgccaggt ttttggggta tctaactaat
     3481 gaaggggtaa aggggacgcc tgtcgctaag atatagaaat gaaaaattac ttgacgacct
     3541 ttagaaaact gtccaggtta taaggggacg atacaaaaca gatcattagc gatgcatccg
     3601 gtctcgcaat attatgctcc ataggattat ttggacttgc ccatttctcc acgataaaac
     3661 gtaccaagga aataggtatt cgcaaggtat tgggtgcatc ggttattaat atctcaactt
     3721 ttctatcgaa ggacttctta caacttgtat tgctttctgt aattatcgcg cacggttgga
     3781 tgggcccgaa gcaacagggg gaaaccggcg cgtgaccggg gaggacacct cgggggcatt
     3841 aaaaaaaagc tattttattt tggattccgc gcccagatgg gacgtcgtca tagacggata
     3901 atagaaatca ccttttaagg ttttagacaa gtaagaaatc agaaaaatac ctacaattcg
     3961 gttatatttt atatttttgc atgaattatc ggtatttatg agtggaaaga aaacggcctt
     4021 gttaccaggc ccggaaaaaa tccttacgga attgggtgtc aatctaaaat tggcacggaa
     4081 gcgcaggaaa ttgacccgca acttggtggc ggagcgtgcg ggcattgcac gctcgacact
     4141 acagcttatt gaggatggga atccaggggt agcaatgagc agttatctgc aagtgctgtt
     4201 tgtgctgggc ctggagaagg atctattacg ggtagcagcg gatgatctgc tcggtcggaa
     4261 attgcaggat gcggctttat tgaataaaaa gaaaggtaat gacaaagcaa attgaagttt
     4321 acgcagattg gattggcttg aacgggccag agcacatagg gcggctgaca gcgcagtcga
     4381 tccgggggcg ggaaatcttt tccttctctt accgaccgga atggttggaa gttgctgggg
     4441 cgttggtgct cgacccggac ttacagcttt tcaccggcgc acaatacgtg cgcgacgaga
     4501 aaccgaattt tggtttgttt actgattccg cacccgaccg ttggggacgt gtactaatgc
     4561 agcggcgcga ggcgctattg gcaaagaagg aagaaagaag gccgcgcacc ttgatggaaa
     4621 gtgactattt gttgggtgtt tttgacgcct atcgcctggg cgcccttcga ttcaagttag
     4681 ataaggacgg gccattcctc gatgatcagg cgacgatggc tgccccgccg atgacggcgt
     4741 tgcgcacatt ggaggaggca agcttgcatt tagaacagga aaatgcggcg gatgacccgg
     4801 cgtttggggt ttggcttaat caacttttat cgcccggtgc gagtcttggc ggggctcggc
     4861 caaaggccag cgtaattgat ccggaaggac gtttatggat tgccaaattc ccaagcggag
     4921 gcgacgaccg ggatataggc ggatgggaag cggtagtaaa tgaattggca ctgcgggcgg
     4981 gcctgaatgt cgcacaaggg caagcgaagc ggctaaccct acaacatcat acgtttttaa
     5041 cccggcgttt cgatagagac ggtaattggc gtatccactt tgcatccgcg atgaccttgc
     5101 ttggacggac agatggggcg gatgcagcta cctcatccta cctggaattg gcggaatgca
     5161 tcgcaagagt aggtgcccga acaaactccg acctcgaaga attgtggcgt aggatagtct
     5221 tcaatatctg tataagtaat gcagacgacc atttgcgcaa ccacggtttc ctcttgacgt
     5281 ctaaaggttg ggttctgtcg cctgcttacg atctgaaccc agtgcccgat gcgcaaggat
     5341 taacgttgaa tattgacggc accaataatg cgctggatat ggagttagca ggaagcgtcg
     5401 cgccacaatt ccgggtcaag tcagagcgga gtaaacagat tattcaagag gttaccaagg
     5461 cagtcaatgg atggtcggcg ctggctgcaa aaatgggaat accgagggcg gagcgggaga
     5521 gggtcggagt ggcgtttgat aaggtctcaa accgttcata aaaatcgttg attctaggcc
     5581 gcctgttctc ctgcctcctg accgaatgac ttagtcagcc gagtctgtta aatcttgaac
     5641 atagtccaat ccatttcaac ctcataccca taattaagcc tgaagctttc gcataacatc
     5701 cctcggaggc taggctataa tattcttcgc cagtgagttt ggtactggcg ttgatatgta
     5761 tcgctttgat cttgatcatc ggtctggtgt cgaaatagcc tttgggcagg acctgtgtcc
     5821 ggtcccattc aggcttgccg aaacttttca caaaagagga ttcattatcg acccataccg
     5881 gtagctgcag atacctggtg aacctgttcc ggccggggct aaggcgccat tcgaacttag
     5941 tgaatttttc gccctcttcc agatcctgta cgaatgaaaa aggatcacat gatgtgatcc
     6001 tcgcggtagc ggactggacg ggaccgtcca gcctatcaca tcttactgat tttcagattc
     6061 ctattttttc attcgcggtt tcattcgcgg tggcgctcca ctccgttttt cactacaaaa
     6121 tcatacggtt tcgaacacgc taaaacaaag gtaagccatc ggccctaact gccgcgcgaa
     6181 ttttccggcc aaatacgggc aaaaaaaagc cgcctggcag gcagggcgga ctgtataatg
     6241 gagcaagtgc tacgaggtgt tacttcgagt tctcttgcca gagtgcctcg atattggtcc
     6301 agagaaaata caccttccct ttcatcttga caggatgcaa ttttcccgct ttaatccact
     6361 cgtaaatggt ggaccgggaa
//
LOCUS       DATGKE010000197         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_27661, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000197 DATGKE010000000
VERSION     DATGKE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27661"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VKU83_03445"
     CDS             complement(<1..347)
                     /locus_tag="VKU83_03445"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLI92630.1"
                     /translation="MKPKLLLAGALLSSIIVTAQTPAFIADSLDGYIQQGMKEWQVPG
                     LALVIVKDGKVVVMKGYGVKDIQTKEPVDENTLFMIASNTKLFTATALAQLESDKKLS
                     LDDKITKYYPDFKL"
     gene            500..688
                     /locus_tag="VKU83_03450"
     CDS             500..688
                     /locus_tag="VKU83_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92631.1"
                     /translation="MKHTIKNVLSIGLFIATIAFSLSSCYEGRYYNHYHHHTHGWYDR
                     HHQAPPPGVRWDVDVDLH"
     gene            813..1850
                     /locus_tag="VKU83_03455"
     CDS             813..1850
                     /locus_tag="VKU83_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI92632.1"
                     /translation="MSRNILLPLLCAFLMPGISLLNNNVFSSAVEPRRVIGSWLIGFV
                     FLLLLWAIDQWIADRFNTANRAAEPKSAVSRPAAVHGPPALRLAVQILVNLILTVGVI
                     RLLGAVDGLRINVHATPTLGFIKLTLASLVILSIQATLASLEEKEDILLANAQLQNEN
                     LRTRFEMLKQQVNPHFLFNALSTLRIMIREKDERAEPFILKLSDLYRQLLGKNTGQTI
                     ALGDELEFLKSYIYMLQARFESLLHIDIDVWPDSLTRKVPAFSLQLLIENCIKHNIIS
                     AARPLYIRIFQRQEDRIIIANNLQIRQPAEESSGIGLDNLRKRYDLLQVREGVTIDRS
                     NETFSVTLQLL"
     gene            1851..>2450
                     /locus_tag="VKU83_03460"
     CDS             1851..>2450
                     /locus_tag="VKU83_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI92633.1"
                     /translation="MNILIVEDEPRTARLLREFIVALDDANTVLEICDSVESTVQFLQ
                     KNSQRLDLLFMDIQLADGSSFEVFKKVQVATPVIFCTAYEDHLLEAFKSNGIDYILKP
                     FREKDIEQAFRKLDQIRESLLLTPSLPLRIEHAFASRAQSSFLVKFRDKMYPAAVRDI
                     AAVALEKEVVYLYTFNKEKHAVFRPIDDIEAALDPQAFFR"
BASE COUNT          551 a          756 c          573 g          570 t
ORIGIN      
        1 agtttaaaat cggggtaata cttcgtgatc ttgtcgtcga gcgaaagctt tttgtcggat
       61 tcaagctggg ccagggccgt cgcggtaaac aatttggtgt tgctggcgat catgaacagc
      121 gtattctcgt ccaccggctc ctttgtctgg atgtccttca cgccatagcc cttcatgacc
      181 acgactttcc cgtccttgac aataacgagc gccagtcccg ggacctgcca ctctttcatc
      241 ccctgttgaa tataaccgtc gaggctgtct gcgataaaag ccggggtctg ggccgtgact
      301 ataattgacg aaagcagcgc gccggcaagc aaaagttttg gtttcatggt tacaaatata
      361 atgttccggg ctttctcgaa tttcaaccgg gggcggcaaa taaatccaca agtccaccgg
      421 ccagcatcta ttaacttcct gtttacccgt gctttgcgtc tggcaggatt ttagacaaga
      481 tacttcaaaa accatttata tgaagcacac gatcaaaaac gtgctgagca tcggcctttt
      541 catagccacc atcgccttct cgctatccag ttgctatgaa ggccggtact acaaccacta
      601 tcaccaccac acacacggct ggtacgaccg gcaccaccag gcaccgccgc cgggggtccg
      661 gtgggatgtg gatgtcgatc tgcattaata tcatcccgag ctttcaactg acccggcatt
      721 agcgggtcat ttttttatgt tatcccggct aaatatcttc ggtatccaaa actcatccgt
      781 tgccaaccgc catccggcaa aagcctaagt tcatgagcag aaacatcctg ctcccgctat
      841 tgtgcgcctt cctgatgccg ggcatcagcc ttttgaataa caacgtattt tcttctgccg
      901 tcgagccccg ccgtgtcatc ggctcctggc tcatcggctt tgtattcctg cttttgcttt
      961 gggcgatcga ccaatggatt gccgaccggt tcaacacagc caatcgcgcc gcggagccga
     1021 aaagcgcggt gagccgccct gccgccgttc acggtcctcc cgcccttcgc cttgccgttc
     1081 aaatcctggt caacctgatc ctgaccgtcg gcgtcatccg cctgctgggt gcggtcgatg
     1141 gcctccgcat caatgtgcac gccacgccta cgctcggttt catcaagcta accctggcct
     1201 ccctcgttat cctttcgatc caggcgaccc ttgcctccct cgaggagaaa gaagacatcc
     1261 tgctggcaaa cgcgcagcta cagaacgaga acctccgtac gcgcttcgaa atgctgaagc
     1321 agcaggttaa tccgcatttt ctgttcaacg ccctcagcac gctccgcatc atgatccggg
     1381 aaaaggatga acgcgccgag ccctttatcc tgaaactctc cgacctctac cgccagctgc
     1441 tcggcaaaaa cacgggtcaa accatcgccc tgggggacga gctcgagttc ctgaaatcat
     1501 atatctacat gctgcaggcg cgcttcgaat cccttcttca tatcgacatc gacgtctggc
     1561 cggacagcct tacacgaaag gttcccgcat tcagccttca gcttctgatc gaaaactgca
     1621 ttaagcacaa catcatctcg gcggcccgcc ccctttatat ccgtatcttt caacgccagg
     1681 aggaccgcat catcatcgca aacaacctgc aaatccggca gcccgccgaa gagtcttcgg
     1741 gcatcggtct cgacaacctg cgcaaacgct atgacctgct gcaggtccgg gagggtgtga
     1801 cgatcgatcg cagcaatgaa accttttccg taacccttca actcctgtaa atgaatatcc
     1861 ttattgtaga agacgagccc cgtaccgcgc gcctgctgcg ggaatttatt gtagcgcttg
     1921 acgatgccaa cacggtcctt gagatctgcg acagcgtcga gagcacggtg cagttccttc
     1981 aaaagaacag ccagcggctg gacctcctgt tcatggacat ccagctggct gacggtagca
     2041 gcttcgaggt ttttaaaaag gtgcaggtgg caacgccggt tatattttgc acggcatatg
     2101 aagaccacct gctggaagcc ttcaaatcga acggcatcga ttatattctc aaaccgtttc
     2161 gcgagaagga catcgaacag gcatttcgaa aactcgacca gatccgtgaa tccctcctcc
     2221 tgaccccgag ccttcccctt cgcatcgagc acgcttttgc cagccgcgcc cagtcctcct
     2281 tccttgtcaa attccgcgac aagatgtacc ctgccgccgt ccgcgatatc gcggctgtag
     2341 ccctcgaaaa ggaggtggta tacctgtaca cgttcaacaa ggaaaaacac gccgttttcc
     2401 ggcccatcga tgatatcgag gcggccctcg atccacaagc cttcttccgg
//
LOCUS       DATGKE010000198         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_378425, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000198 DATGKE010000000
VERSION     DATGKE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378425"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VKU83_03465"
     CDS             <1..195
                     /locus_tag="VKU83_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92634.1"
                     /translation="RDNLGALRGFRAELDGKWLCFTNDKYLAYIYRFDAHCPRGPHVL
                     KVSVEDIAGNRRETQYHFYR"
     gene            198..677
                     /gene="bcp"
                     /locus_tag="VKU83_03470"
     CDS             198..677
                     /gene="bcp"
                     /locus_tag="VKU83_03470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HLI92635.1"
                     /translation="MTTLKVGETAPAFAGKDQDGNPVTLQDYKGKKLVLYFYPQDDTP
                     ACTDEACNLRDNYALLKKAGFAVVGVSPDDEKSHKKFETKFGLPFPLIADPGHVIIDR
                     YGVWGEKQLYGRKYVGLHRTSFLIDERGVIRKIFLRPRNKQHAQEILKAWAEMTKTR"
     gene            complement(664..1407)
                     /locus_tag="VKU83_03475"
     CDS             complement(664..1407)
                     /locus_tag="VKU83_03475"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLI92636.1"
                     /translation="MAIGHAFIELESVDSTNNYAMARATSGQASHGTIFFARRQWAGK
                     GQRGRSWNSKPGENIILSAVLEPVAFAPSQAFPLSAAVALACDDLFTRHAGADMTRIK
                     WPNDLYWGDRKAGGILIENNLRGERWAYAIVGIGININQTDFPDTGGNPVSLRQITGR
                     PADPMQLARELAACLERRFTELESDPTAIIARYNSRLYGLGREVTLRAKEGILRTIVT
                     GVSADGKLLTRDSAERSFTFGEVDWIIGS"
     gene            1550..1903
                     /gene="rsfS"
                     /locus_tag="VKU83_03480"
     CDS             1550..1903
                     /gene="rsfS"
                     /locus_tag="VKU83_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HLI92637.1"
                     /translation="MTHHSKIFKTIIQAIQEKKAQHIISLDLRKIPEAVADFFVICEA
                     SSTTQVKAIADSVEEEVKKHCEELPYKHEGRTSLQWVLIDYVNVVVHVMLPESRRFYK
                     LEDMWSDAPQEKYGN"
     gene            2026..>2898
                     /locus_tag="VKU83_03485"
     CDS             2026..>2898
                     /locus_tag="VKU83_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLI92638.1"
                     /translation="MPPEDLKQNDKNNFPRFRPRGDDDNGNRKGPKFSIYWIWGIIAA
                     VLIGFNLFGTFTPDAHQVEELEFRKNMLAQGDVEKIDLISNKNIVRIYIKQDSLKKPF
                     YRQYLNKGNWPGGVSKEGPQFEFRVTDVKEYERWLRDYFAKNPSQEVDINSKPEGEWF
                     GPLLNFLLPLLIIVLIWVMLMRKMGGQAGAGGPGGIFNIGKSKATLFDKGTKVTITFN
                     DVAGLDEAKVEVMEIVDFLKNPKKYTALGGKIPKGALLVGPPGTGKTLLAKAMAGEAQ
                     VPFFSMSGSDFVELF"
BASE COUNT          708 a          747 c          818 g          625 t
ORIGIN      
        1 agggacaacc tcggcgcgct ccgcgggttt cgcgcggagc tggacgggaa atggctttgc
       61 tttaccaacg acaagtatct tgcctatatt tacaggtttg acgcgcattg cccgcggggc
      121 ccgcacgtgc tgaaagtatc cgtggaggac atcgccggga atcgccgtga aacgcaatat
      181 catttttacc gataagcatg acaacactaa aagtaggcga gacagcgccg gcctttgccg
      241 gtaaggacca ggacggcaat ccggtcaccc ttcaggacta taagggaaaa aagctggtcc
      301 tgtattttta tccgcaggac gacaccccgg cgtgcaccga cgaggcctgc aatctccggg
      361 acaattacgc gctgctgaaa aaggcgggat tcgctgtcgt cggcgtcagt ccggacgacg
      421 agaagagcca taagaagttc gaaacgaagt tcgggctgcc ttttcccctc atcgcggatc
      481 ccggtcatgt catcatcgac cgttacggcg tgtgggggga gaagcagctc tacggccgga
      541 agtatgtggg gttgcaccgg accagttttc tgatcgacga gcgcggcgtc atcaggaaga
      601 tctttctccg gccgaggaac aagcagcatg cacaggagat cctgaaggcc tgggcggaga
      661 tgactaagac ccgatgatcc aatcgacctc gccgaacgta aaggaccttt ccgccgaatc
      721 ccttgtgagc agtttgccgt ccgctgacac gccggttacg atggtccgga ggatcccttc
      781 cttcgcccgg agcgtgactt cgcggccaag gccatagagc ctgctgttgt agcgggcgat
      841 gatcgcggtc gggtccgatt ccagttcggt aaagcgacgc tccaggcagg cggcgagttc
      901 cctggcaagc tgcatcgggt cggccgggcg gcccgttatc tggcggagcg aaaccgggtt
      961 gccgccggtg tcgggaaaat cggtttggtt gatgttgatc ccgatgccaa cgatggcata
     1021 ggcccagcgt tcgccgcgga gattgttttc gatcagaatg cctcctgcct ttctgtcacc
     1081 ccaatacagg tcgttgggcc acttgatccg ggtcatgtca gcgccggcgt ggcgggtgaa
     1141 aagatcgtcg caggccagcg caacggcggc gctcaacggg aaggcttgcg acggagcaaa
     1201 tgcaactggc tctaacacag cactgaggat gatgttctcc cccggcttgc tgttccagct
     1261 gcggcctctt tggcctttgc cggcccactg gcgcctcgcg aagaagatcg tcccgtgact
     1321 ggcctggccg gaggttgccc gggccatggc atagttgttg gtgctgtcca ccgattctaa
     1381 ttcaataaaa gcatgaccga tcgccaaaag attcctattt ttgatgagga taaagataac
     1441 ccgtcatctc acaatattct gatttgcacc taatttatca taaaaaccac attgattatt
     1501 ggaacagctg tctatattag ctacccgcaa acgcagcagc gtagtaaggt tgactcatca
     1561 ttcgaagatc tttaaaacaa tcattcaggc gatacaggaa aaaaaagcac agcatatcat
     1621 ttccctcgat ttgcgaaaaa tcccggaagc agtggcggat tttttcgtta tatgtgaggc
     1681 cagtagcacc acccaggtca aagcgatagc cgattccgtt gaagaagaag taaagaaaca
     1741 ttgcgaagag cttccctata aacacgaagg ccggacttcc ctacaatggg tattgatcga
     1801 ctacgtcaac gtggtcgttc acgtcatgct gccggagagc cgccgttttt acaagcttga
     1861 ggacatgtgg agcgacgccc cccaggagaa gtatggaaac tagaaagcct gctactggca
     1921 atttaacagg ttattttgcc gtttcatgga tttttttgaa catttttacc ttacaatcat
     1981 ttattatatt taataatatc cagcaaacaa cacgcatctt acaaaatgcc acccgaagat
     2041 ttgaaacaaa acgataagaa taattttccc aggttccggc ccagggggga cgacgacaat
     2101 gggaaccgga aggggccaaa gtttagcatt tattggattt ggggcatcat tgcagccgtt
     2161 ctgatcggtt ttaatctgtt tggaaccttt actcccgacg cgcaccaggt cgaggagctc
     2221 gaattcagga agaacatgct ggctcagggg gacgtggaaa agatcgacct gatctccaac
     2281 aagaacattg tcaggatcta tatcaagcag gacagcctta aaaaaccttt ttaccgtcaa
     2341 tatcttaaca agggcaactg gcccggcggc gtcagcaaag aaggtccgca attcgagttc
     2401 cgggtgacgg acgtgaagga atacgagcgg tggctgcgcg actattttgc aaagaacccg
     2461 tcccaggagg tggacatcaa ctcgaagccg gagggcgagt ggttcgggcc tttgctcaac
     2521 ttcctgcttc cgctgctgat catcgtgctg atctgggtca tgctcatgcg aaaaatgggc
     2581 gggcaggcag gggccggagg cccgggcggc atcttcaata tcggcaaatc aaaagcaacg
     2641 ctattcgata aggggaccaa ggtaacgatc accttcaacg acgtggcggg cctggacgag
     2701 gccaaagtcg aagtcatgga gatcgtcgac ttcctgaaga atcccaaaaa atatacggcg
     2761 cttggcggga agatcccgaa gggcgcgttg ctggtgggac cgccgggaac cgggaagaca
     2821 ttgctggcga aagccatggc gggtgaagcg caggtgccgt tcttctccat gagcgggtcc
     2881 gacttcgtcg aactcttc
//
LOCUS       DATGKE010000199         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_649273, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000199 DATGKE010000000
VERSION     DATGKE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649273"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VKU83_03490"
     CDS             complement(<1..353)
                     /locus_tag="VKU83_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HLI92639.1"
                     /translation="MPHESISVNDIFKIGIGPSSSHTLGPWRAAQRFTDSIHRRHGLH
                     GLETIQVLLYGSLAKTGKGHGTDIAVQLGLAGEDPVTFDTGLIDSTIQAIRASGRLRV
                     RGEQPVEFNPETDIR"
     gene            complement(393..1565)
                     /locus_tag="VKU83_03495"
     CDS             complement(393..1565)
                     /locus_tag="VKU83_03495"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLI92640.1"
                     /translation="MSKRPGYLTNLTPLRGLAALLVAIFHFEMTAGRFIPAGSTMFIE
                     KSYLMVDLFFVLSGFIMLHVYGRAFDGTMQNGNLRHFFVARFARTYPLHLFSLLLLVV
                     IVRYLTNWGNPPILIEQPRDILPNVFLLQAWGVCKIFNWNIPSWSISAEWAAYLIFPF
                     LALWFSRRRRAAPILLAAGVVVAWCSIMYLLPRKNPFNPLIAVPHNLNTTYDYGWLRG
                     IAGFCAGMVTYRFYQRPGTGRMMGGDGASLLILLLVAAALHFAVNDALVVLLFAALVL
                     NFSLNNGWLHRICNFRPLQYLGDISYSIYLMQIFLQEPFSHGIRLPGVTGTARGKFNF
                     THFWPGLGFCLLYVLLLIGIASVTYFLVERPCRRWINQRWGQPRIPKAVSGLKTEP"
     gene            complement(1569..2606)
                     /locus_tag="VKU83_03500"
     CDS             complement(1569..2606)
                     /locus_tag="VKU83_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLI92641.1"
                     /translation="MLRAIPSGFPAILISLVMTLPVAAQPGAASNPYVLDASASTVVW
                     GYYWSEAKPVLHIHSGDYVRVRTVLTSSPDRLEAAGVPAADVEQQLRDVQAVKDRGPG
                     GHVLTGPIYVEGADSGDVLEVRFDSINLAIPYGYNGIGQAGFLSDEIFSRKMRIIPLD
                     RDRMTGHFSAGIDIPLHPFFGSAGVAPPAYAGRWNSAPPWIHGGNLDNKELVAGTALY
                     LPVFVKGALFEIGDGHAAQGNGEVDITAIETSLIGKLQFIVHKGRSIPWPWAESPTHI
                     ITMGEDRELNAATHIAVRQMIRYLMAERNMTQEDAYMLCSVAADVDITELVDGNVGVH
                     VMLPKSVFTTK"
     gene            2672..3940
                     /locus_tag="VKU83_03505"
     CDS             2672..3940
                     /locus_tag="VKU83_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI92642.1"
                     /translation="MSPATLKIAICLMGILLAGQVRGQQRPVHSNIALVGARIYPSPT
                     DSPIDNGVVLIRNGKIAAVGPAGAIKVPPSYSVVDCKDLSLTAAFWNCHVHFIEPKWQ
                     DADLMPEAQFNQQMEQMITSHGFAHVYDLAEFAIGNVLRIRNRIKRGDVRGPVIYTTG
                     VPLVPQDGSPFYIAPRKLPEATDPEQAVTHVREQIDSGADGVKIWTGSPTGNKVVYMP
                     GEIVRAITETAHRLRKPVFAHPTDTKGVMIAVDNGVDILAHTSADDGRLWSEDTIERM
                     LAAHIALIPTLKLWKDELKKAGVTDWAHHPLVLTAQQQVHDFSKAGGVVLFGTDVGYL
                     SDYATSDEYVLLAGAGLNFPQILAMLTTAPAKRFGLARRTGRIAVGMDADIVLLAGDP
                     AKDIQALDKVAYTFLKGRIIYTSAPPDTTP"
     gene            complement(3937..>4444)
                     /locus_tag="VKU83_03510"
     CDS             complement(3937..>4444)
                     /locus_tag="VKU83_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HLI92643.1"
                     /translation="GQPGAEYDLSVTAGGETYRAVSAMPMPVTLDSVSFQSNSGFGRT
                     RISALANFRDPAGVRNYYQFVEYVNGARLSKELYVFDDRLSDGRYINYTLFNDSTYLQ
                     AGDQVQVDMYCIDSTVYNYFFKLFQSGGAGAFNTSASPANPTSNLSNGAFGYFSAHTV
                     SSRSGYVP"
BASE COUNT          994 a         1286 c         1318 g          846 t
ORIGIN      
        1 aagcggatat cggtctcggg attgaattcg accggctgct caccgcggac ccgcagccgt
       61 ccgctggcgc ggatcgcctg gatcgtggaa tcgatcagcc cggtatcgaa agtcaccggg
      121 tcttcgcctg ccagtccgag ctgcacggcg atatcggtgc catgtccttt tccggttttc
      181 gccagggagc cgtacaagag cacctggatc gtttccagtc catgaagtcc atgccggcga
      241 tgaatagaat cggtaaatcg ctgcgccgcg cgccacggac ctaaagtgtg ggagctggaa
      301 gggccgatcc cgatcttgaa aatatcgttg acggaaattg attcgtgtgg caagtcgcgt
      361 tgattttcag gcaaagttat tacgttttcc cattatggct ccgttttcag gccggagacg
      421 gctttcggga tccgcggctg gccccatcgt tgattgatcc agcgccggca gggccgctcg
      481 actaggaagt aagttacgga agcgatcccg atcagcaata acacgtacag gagacagaag
      541 ccgaggcccg gccagaaatg ggtgaaattg aattttcccc tggcggtacc ggtgaccccg
      601 ggcaggcgga tcccatgaga gaacggctcc tgcaaaaaga tctgcatgag gtagatcgag
      661 taggaaatat cgccgaggta ctgcagcggc ctgaagttac agatccggtg aagccacccg
      721 ttattcaggg aaaaattcag caccagcgct gcgaacagga ggacgacaag ggcatcgttg
      781 accgcgaaat gcaacgcggc ggccacaaga agcaggatca ggagggaagc gccatctcca
      841 cccatcatgc ggccggtacc gggcctttga taaaaccggt aggtgaccat gccggcgcaa
      901 aatcccgcga tgccccgtag ccagccgtag tcgtaggtgg tgttcaggtt atgcggtacc
      961 gcgatgagtg gattgaaggg gtttttccgg ggcagcaggt acatgatgct gcaccaggcc
     1021 acaaccacgc ccgcggccag caggatgggc gcagcccgcc ggcggcggct gaaccacaac
     1081 gccagaaatg gaaagatcag gtaggctgcc cattcggcgc tgatactcca ggaaggaata
     1141 ttccaattga agattttaca gactccccat gcctgcagca gaaagacgtt cggcaggatg
     1201 tcgcgcggct gctcgataag gatcggcgga ttgccccagt tggtgaggta tctgacgatg
     1261 acgaccagca gcaagaggga aaaaaggtgc aagggatagg tgcgggcaaa ccttgccaca
     1321 aagaaatggc gcaggtttcc attttgcatg gttccgtcaa aggctcgccc atatacgtga
     1381 agcatgataa acccgctcag tacgaaaaag aggtctacca tcaggtagga cttttcgatg
     1441 aacatcgtgc tgcccgcagg aatgaaacgg ccggccgtca tttcgaaatg gaagatcgct
     1501 accagcaatg cggcaaggcc gcgcaggggc gtcagattgg taaggtaccc gggtcgtttc
     1561 gacataactt attttgtggt aaaaacggat ttcggaagca tgacgtgtac gccgacgttg
     1621 ccgtcgacca attcggtgat gtccacatcg gccgctacgc tgcagagcat atacgcgtct
     1681 tcctgcgtca tattcctctc cgccatcagg taccggatca tctgccgcac cgcaatatgc
     1741 gtcgcggcgt tcaattcccg atcctcgccc atggtgatga tatgcgtcgg cgactccgcc
     1801 catggccacg gaatgctcct gcccttatgc acaataaatt gcagctttcc gatcagcgag
     1861 gtctcgatcg cagtgatatc gacctcgccg ttgccctgtg cggcatgccc atcgccgatc
     1921 tcaaacaacg cgcctttgac aaatacgggc agatagagcg ccgtgcccgc aaccagttcc
     1981 ttattgtcga gattgccgcc gtggatccag ggcggtgcgc tgttccaccg gccggcgtag
     2041 gcgggtggcg ccacgccggc actgccaaaa aacggatgca gcgggatatc tatccccgcc
     2101 gaaaaatgtc ctgtcatgcg atcccggtca agcgggatga tccgcatctt cctgctgaag
     2161 atctcgtcgg agaggaaacc cgcctggccg atgccgttat atccgtacgg gatcgccagg
     2221 ttaatggagt cgaaccgcac ctccagcacg tcgccggaat cggccccttc tacgtagatc
     2281 gggccggtaa ggacgtgacc gccaggccca cggtctttga ccgcctgcac gtcgcgtagc
     2341 tgctgctcca cgtccgccgc aggcacaccc gccgcctcca gacggtcagg gctcgacgtc
     2401 agcaccgtgc ggacccggac ataatcgccg gaatggatat gcagcaccgg cttggcctcc
     2461 gaccaataat agccccagac gaccgttgat gccgaagcat cgagcacata aggattggaa
     2521 gccgctccgg gctgcgcggc aacgggaagg gtcatgacga ggctgatcag tatcgccggg
     2581 aagccggaag gaatggcacg cagcatcgga tacaaaattt atatcgatcg tcgaccgggc
     2641 aaatgtatcg attttgcata aatttatcgg catgagtcct gcaaccttga aaatcgcgat
     2701 ctgcctcatg ggcatcctgc tggccggcca ggtccgtgga cagcagcgtc ccgttcattc
     2761 caacatcgcg ttggtcggcg ccaggatcta tccttcccct acggatagtc cgatcgacaa
     2821 cggtgtcgtt ctgatccgca acggaaagat cgccgcggtt gggccagccg gcgccatcaa
     2881 agtgcccccg tcctattccg tcgtcgattg taaagacctc agccttacgg cggctttctg
     2941 gaactgtcat gtccacttca tcgaaccgaa atggcaggat gccgatctga tgccggaggc
     3001 gcagttcaat cagcagatgg agcagatgat caccagccac gggttcgcgc acgtgtacga
     3061 cctcgcggag tttgcgatcg gaaatgtcct ccgcatcagg aaccgcatca agcgcggtga
     3121 cgtccgcggc ccggtcattt acacgacggg ggttcccctc gtgccacagg acggcagccc
     3181 gttttatatc gcgccgcgaa aactgccgga ggcaaccgat ccggagcagg cggtcaccca
     3241 tgtacgcgag cagatcgact cgggcgccga cggggtgaag atttggaccg gttcaccgac
     3301 gggcaacaag gtcgtctata tgccgggcga gatcgtccgc gcaattacgg aaacggccca
     3361 ccggctcagg aagccggtgt tcgcccatcc caccgacacg aagggggtca tgatcgccgt
     3421 ggacaacggc gttgatatcc tcgcccatac ctcggccgac gacggccggc tctggagtga
     3481 agataccatc gaaaggatgc tggcggcgca tattgccctt atcccgacgc tcaaactctg
     3541 gaaggacgaa ttgaaaaagg cgggggtaac ggattgggcg caccatccgc tggtgctgac
     3601 ggcacagcag caggtgcacg atttctcgaa agcggggggc gtcgtcctgt tcggcaccga
     3661 cgtaggctac ctcagcgact acgccaccag cgacgaatat gtgctcctgg ccggcgcggg
     3721 actcaatttt ccacagatcc tcgcgatgct gaccacggca ccggcgaaac gcttcggtct
     3781 tgcgcgccgg acaggcagga tcgcggtcgg gatggatgcc gacatcgtct tgctggcggg
     3841 cgatcccgcg aaagatatcc aggcgctcga taaggtcgcc tacacttttc tgaaaggcag
     3901 gatcatctac acctccgcgc cgcctgatac gacgccttaa ggcacatacc cggaccggga
     3961 gctgaccgta tgcgcgctga aataaccaaa ggcgccgttg ctgagattgg aggtcgggtt
     4021 ggcaggggaa gccgacgtat tgaacgcacc tgcaccgccg gattgaaaga gcttaaaaaa
     4081 atagttgtat acggtggagt caatacaata catatcgacc tgtacctggt cgccggcctg
     4141 caggtaggtg ctgtcattaa acagggtata gttgatatat cttccatcgg agagccggtc
     4201 atcgaagaca tacagctcct tcgacaaccg cgcgccgttg acgtactcga caaactggta
     4261 gtaattcctc actcctgccg ggtcccggaa attggcaagg gcgctgatgc gggtccggcc
     4321 aaaaccgctg ttgctctgga agctgaccga gtcgagggtc acgggcatcg gcatggcaga
     4381 gaccgcgcgg tacgtctcgc cgccggccgt taccgataag tcatattccg cacccggttg
     4441 gcct
//
LOCUS       DATGKE010000200         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_318182, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000200 DATGKE010000000
VERSION     DATGKE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318182"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="VKU83_03515"
     CDS             <1..2154
                     /locus_tag="VKU83_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92644.1"
                     /translation="LLIFWGILFSTLNGDFMHSYGAGALFLSPEYLGNVSPVSTSFVG
                     MATGIFIMSWNITTFILFSRHFRFLATTTNPFLKYCMNNFVLPLMFLLFYLVKAIQFS
                     GSKELLPVSSIILLILGFLSGLSFVIMISFLYFFRADKTIVRRMAPVISNPKLFKSQF
                     GREEVKLNETRLMHVKWYLNSPSTIRQTRDVAHYSKAFIESIFNRHHISAVLSIFAAF
                     IFLIVVGFFMDSPYFQLPAAASILIFFAILISLSGAVSYFLGSWAIPFFAVTFVVLNV
                     LYQYHVIDPTNKAYGLNYSSVEDRPLYDRQTLLQLCSETHVGRDEANMIRILERWKAR
                     QSSPRPTLYVINSSGGGNRSACFNMSVLQHLDSLCGGKLMRKTFMMTGASGGMLGAAY
                     FRELSRRRDEGDTSIHIPNRRYVNDISRDLLNTLFSSFVARDLASPAQKFKVGQFEYL
                     KDRGYAFEQQLNINTRGMLNLQLKDLAPEESAAKTPLLLMSPDITQDSRALVISTQPV
                     SFLMRPQYDSTRISPMDPDAVDFGALFARQGPMNLRLLTALRMNASFPYVLPDVWLPT
                     QPVIDVMDAGFRDNFGELSTIRFLYFFRRWIRENTSGVVLLQIRDRKSGGWDAYEPRD
                     ITDIITKPILLLEDNWYNLQEYNQNDLVSLAQDAFGFPFRKLVFQYVPKSEDAGAALN
                     FHLTTQEKLTIIGSLDNEDNQQAFAKFAALANTNH"
BASE COUNT          474 a          636 c          608 g          568 t
ORIGIN      
        1 ctgctgatct tctggggtat tctgttcagt acgctgaacg gagatttcat gcatagctat
       61 ggcgcggggg cgctgttcct gtcgcccgag tacctcggga acgtcagccc ggtcagcact
      121 tcgtttgtcg ggatggccac cggaattttc atcatgagct ggaatatcac cacctttata
      181 cttttcagcc ggcacttccg tttcctggcc acgacgacca atccctttct aaaatattgc
      241 atgaacaatt tcgtgttgcc gctgatgttc ctcctgttct acctcgtgaa ggcgattcag
      301 ttcagcggga gcaaggaatt gttgccggtt tcctccatca tcctgctcat cctgggtttc
      361 ctctcgggcc tcagtttcgt tatcatgatc tcctttttgt attttttccg ggcagacaag
      421 acgatcgtcc ggcggatggc ccccgtaatc agcaatccga agctgtttaa gtcccagttc
      481 ggcagggagg aggtgaagct gaacgagacc cgcctcatgc acgttaaatg gtatctcaat
      541 tcgcccagca ccatccggca gacaagggat gtagcccatt acagcaaggc gttcattgaa
      601 agtattttca accgacatca catctccgcg gtcctttcga ttttcgccgc cttcattttt
      661 ctgatagtcg ttggtttctt catggatagc ccctacttcc aattgcctgc ggccgccagt
      721 atcctcattt tttttgcgat ccttatctcg ctgtccggag ccgtgtccta tttccttgga
      781 tcctgggcca tacccttctt tgccgtcaca ttcgtcgtgc tgaatgttct ctatcaatat
      841 catgttatcg atccgaccaa taaagcctat ggcttgaact attccagcgt agaggaccgc
      901 ccgctttatg accggcaaac gctattgcag ctttgttccg aaactcatgt cgggcgggat
      961 gaggcaaata tgatccgcat cctggagcgc tggaaggccc gccaatcttc gccgcggccg
     1021 acgctttatg tgatcaatag cagcggcggc gggaatcgta gcgcctgttt caacatgagc
     1081 gttctccagc acctggatag cctttgcggt gggaagctca tgagaaagac cttcatgatg
     1141 accggagcgt ccggaggcat gctgggcgcc gcgtatttcc gggagctgtc gcggcggcgc
     1201 gatgagggcg acacgagcat tcatattcca aacaggcggt atgtcaatga tatttcccgc
     1261 gatttgctga ataccctctt ttcctccttc gtcgcgcggg accttgcatc ccctgcgcaa
     1321 aaattcaagg ttggccaatt tgaatatctc aaggaccgtg gctacgcctt cgagcagcag
     1381 ctcaacatta acacgagggg catgctcaac ctgcaattga aggaccttgc gccggaggag
     1441 tcggcggcaa agacaccgct gttgttgatg agcccggata ttacgcagga cagccgggca
     1501 ttggtcatta gcacgcagcc cgtgagcttc ctcatgcgcc cgcagtacga cagtacgcgg
     1561 atctccccga tggatcccga tgccgttgat ttcggcgcct tatttgcgcg acaggggccg
     1621 atgaacctgc gcctgctgac ggcgttgcgg atgaatgcca gctttcccta tgtcctgccg
     1681 gatgtctggc ttccgaccca gccggtgatt gacgtaatgg atgccggttt cagggacaat
     1741 ttcggcgagt tgagcaccat acggttcctc tatttcttcc gcaggtggat ccgtgaaaat
     1801 acgagcggcg tggtgctgtt gcagatccgc gaccggaaat cgggcggttg ggacgcttac
     1861 gagccgaggg atattacgga cattattacc aagccgatcc tgttgctgga agacaactgg
     1921 tataacctgc aggaatacaa ccaaaatgac cttgtcagcc ttgcgcagga cgccttcggt
     1981 ttcccgttcc ggaagcttgt cttccaatat gtgccaaaga gcgaggacgc gggggccgca
     2041 ttgaatttcc atctgaccac ccaggagaaa ctgacgatca tcggctcact ggacaacgag
     2101 gacaaccaac aagccttcgc caaatttgcg gcgctggcaa ataccaatca ttaactacca
     2161 atcattaacg ttggatctta gggctggatc agccggatcg cggaaggcac tacgcggatt
     2221 cggaacaggt cggccgttgg cgctggatcg ccatcgatgt gcagcggcgc ccggccgggg
     2281 ttgacg
//
LOCUS       DATGKE010000201         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1032857, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000201 DATGKE010000000
VERSION     DATGKE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032857"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VKU83_03520"
     CDS             complement(<1..779)
                     /locus_tag="VKU83_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLI92645.1"
                     /translation="MRGLMMDFQLTIPAILRRAESLFSAKEIITRLPDRSIHRYTYAE
                     FAIRAKRLAAALHRLGIREGDRVATLSWNHYGHLEAYFGVPGMGAVIHPLNLRLAPHD
                     LAYIVNHAEDKVIIVDQVLLPLFEQFKAGVDAGTVIVIPQTADPVAPPYLNYEEVVAA
                     GDENEFQPFEGDEYTAAFMCYTSGTTGKPKGVLYSHRSVVLHAMAFLLSCGGLGITER
                     DVVLPVVPMFHASAWGLPYNCAFAGATQVYPGPYLDAESLLA"
     gene            complement(776..1555)
                     /locus_tag="VKU83_03525"
     CDS             complement(776..1555)
                     /locus_tag="VKU83_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HLI92646.1"
                     /translation="MQFHDDISLAQWSLIDEYRTGKLRTLDFPRTARQDFGLNGVELV
                     NTLLEVPTEAYLKQLKQNAEYHGVNIVLLMVDDEGDGCSPTKEGRKEFVTFHRKWVDI
                     AQYLGCHAIRTNCRGAHVTDATDALGWAAESYHPLLEYAKPAGIKILIENHGGVSNDP
                     DWMVRLMKTVDDPFFGTYPDWRQPGPGFDNLEYLKKTLPFAKGMSYRNQPTDELTQKM
                     LGLCRESGFHGWYGIESGGREQIRKGIRLLRAYQQENATIA"
     gene            complement(1673..>2612)
                     /locus_tag="VKU83_03530"
     CDS             complement(1673..>2612)
                     /locus_tag="VKU83_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI92647.1"
                     /translation="FVLTVTDSLGATATDTANILVNPVPQTQSKLINVAIYGFSNPYV
                     NSAWNDWNLGGAVLTSGIFKYSDGTTSTLSAGLSAQSAVVDNGTGYVTVTMCPQQAAR
                     YPSYYSGSGGRTLTLNGLDSTKLYRIDIYATRNNPGQTTTFAVGASKVTIPTNNNTAT
                     VATFDNLTPAPGGKIVVTLTHGQYYDYFNAFTITEKTVVATGGGAPEMTAFTPGITAD
                     TSPVAGNGSLFGEAIGIYPNPLKDGFELHVHNANTGRLKVDIVTAGGVLVKEFVIPKT
                     TPEFDIRLSLAGLKPGDYFLVATIGNWRHSIKLVKL"
BASE COUNT          519 a          720 c          794 g          579 t
ORIGIN      
        1 gcaagcagac tttctgcgtc gaggtagggc cccggataaa cttgtgtagc accggcaaag
       61 gcacaattat agggaaggcc ccaggcgctc gcatgaaaca tgggaacgac gggcagcacg
      121 acatcccttt cggtgatgcc caggcccccg caactcagca ggaaggccat ggcgtggagg
      181 accaccgacc ggtgagagta caacacgcct ttcggcttcc ccgttgtgcc ggaggtatag
      241 cacatgaagg cggcagtgta ctcgtcgcct tcaaagggct gaaattcgtt ttcgtcgccg
      301 gccgcgacta cttcttcgta gttcaggtaa ggcggcgcga cgggatcggc ggtctgcgga
      361 ataacgatga ccgtgccggc atcaacgccg gctttgaatt gctcgaacaa aggaagcagg
      421 acctggtcga cgatgatgac cttgtcttcg gcatggttta caatgtaagc caggtcgtgg
      481 ggagccagcc gcaggttcag cggatggatc accgccccca tgccgggaac accaaaataa
      541 gcctccaggt gtccgtaatg gttccagctc agcgtagcca cgcggtctcc ctcgcgaatg
      601 ccgagccggt gcaaggcggc ggccaggcgt ttggcacgga tggcgaattc cgcgtacgta
      661 taacgatgaa tgcttctatc cggaaggcgg gtgatgatct ccttagctga aaacaatgat
      721 tccgcccgcc ttaggatggc gggaatggtc agctggaaat ccatcattaa ccctctcatg
      781 cgatcgtggc attttcctgc tgataagccc tgagcagccg gatgcccttc cggatttgct
      841 cccgcccgcc cgattcaatg ccataccagc catggaaacc gctttcccgg catagcccga
      901 gcattttctg ggtgagctcg tcggtcggct ggttccggta ggacatgcct ttcgcgaatg
      961 gcaaggtctt tttgagatat tccagattgt caaaccctgg tcccggttgc cgccagtcgg
     1021 ggtaggtgcc gaaaaaggga tcgtctaccg tcttcatcag ccgcaccatc cagtccgggt
     1081 cgttggagac gccgccgtgg ttttcgatca gaattttaat gccggcaggt tttgcgtatt
     1141 cgagcagcgg atgatatgat tcggcagccc agcccagggc gtccgtggca tctgtcacat
     1201 gcgcgccgcg acaattggtg cggatggcat ggcaccccag gtactgcgcg atgtccaccc
     1261 atttccggtg aaaggttacg aattccttcc ggccttcctt tgtcggcgag catccgtcac
     1321 cttcgtcatc gaccatcagc agcacaatgt tgacaccgtg gtattccgcg ttctgtttca
     1381 gctgcttcag gtaggcctcg gtcgggactt ccaggagggt gttgaccagc tcgacgccgt
     1441 tgaggccgaa gtcctgccgc gcggtgcgcg gaaagtcgag ggtccgaagt ttgccggtcc
     1501 ggtattcgtc aatcagggac cattgggcga gggaaatgtc gtcgtggaat tgcatacgtt
     1561 aagttacgcc aaggcccgga ccgaaccaaa tggcgcttgt gctggcaaaa aggcggggga
     1621 catatttgag ggtgatcctt caaactgttc cgcccgccgt tggttttcct aattataatt
     1681 tcaccagttt tatcgaatgt ctccagttgc cgatcgtggc aacgaggaaa tagtcccccg
     1741 gcttcaggcc tgccagagac aggcggatat cgaattcggg agtggttttc gggatcacaa
     1801 actccttcac caggacgccg ccggcagtga cgatatccac cttcagccgg ccggtgttgg
     1861 cgttatggac atgcagttcg aacccgtctt tcaatgggtt gggatagatc ccaatcgctt
     1921 ccccgaaaag ggaaccgttg ccggctaccg gactcgtatc cgcggtgatg cccggcgtga
     1981 atgccgtcat ttcgggagcg ccgccgccgg tggcgacgac ggtcttttcg gtgatggtga
     2041 aagcgttgaa ataatcgtaa tactgtccgt gggtaagcgt gaccacgatc ttgccgcccg
     2101 gcgccggtgt caggttgtca aacgttgcaa cggtcgccgt gttgttgttt gtcgggatcg
     2161 tcaccttgga tgcacccacc gcgaaggtag tcgtctgacc cgggttattg cgggtggcat
     2221 agatgtcgat ccggtaaagc ttggtgctgt ccaggccgtt tagcgtcagg gtgcggccgc
     2281 cggagccgga ataatagctc ggatatcgcg cggcctgctg cgggcacatc gttaccgtga
     2341 catagccggt accgttgtcg acgacggcgg actgcgcgga cagccccgcg gatagggtcg
     2401 aagtcgtgcc gtcggaatat ttgaagatcc ccgaagtcaa tacagcgcca cccaggttcc
     2461 agtcattcca ggcgctgttg acgtatgggt tgctgaatcc gtatatggcg acgttgatca
     2521 gcttgctctg agtctgggga acagggttga cgagtatatt cgccgtatcg gtcgccgtcg
     2581 cgcccaggct gtcggttact gtcaaaacga ag
//
LOCUS       DATGKE010000202         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1271057, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000202 DATGKE010000000
VERSION     DATGKE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271057"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2307
                     /locus_tag="VKU83_03535"
     CDS             <1..2307
                     /locus_tag="VKU83_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HLI92648.1"
                     /translation="RQAGFHTGKSFNKRMILPAILRNWYWFLLAGAVGFGVAFVLRKV
                     FPGSYRSTMTILLINSPRQSPFTSSLDNNLEIQENPINVQDEQTVLSAYSLQLQTLQN
                     LGWKTRWYKKSIFGRKDIYKEDPFAVEFIEDSVLKGVDLLITPLSTTRYRVQCDRKDR
                     VGDTDRVIRFNTVAEFGKPFNNEWFHFTLYPATPPVPANGAQYILDFNDLYFMAIEYQ
                     GNLEVKIGAPESDVLNVELKGENVLRNVDYLNELGKTYQKFGLDQKNQSAINTLTFIR
                     NLISGVADSLRTAGDRYTDFRTRNRVVDLSTESSMVLQKVEDLDKQESLLKLKLDYFN
                     GLTRHLNDGDSLKSFVAPPLGDPDPDLSANVQKLASLYSQRTALSFSVQPKNPRMIAI
                     NDDISLTQRLIQGNIASHLTAIRDQISALEQQKAETNERLTNIPATERSYLDIKRGFD
                     VNSTLYNFLLQKRAEAGIALASNNPDARILDPATLPTTGPIGLKPIVSAAIGVILGML
                     VTLGLVLLNQYLNDRLREPEEAAAALHLSVAGQIPHNRLKTELPTVHHPRSEITESFR
                     NLRANLRYLMKDSTGGVIAIHSASSGEGKSFIAANLASLLALGSKKALLLQADKHNTQ
                     LETLFGAQPRVDLTQYLEGTVSFWEMPASTEIPGLSYIRASQPDGPLSELTDTPAMEK
                     FIREARAGFDFIIIDNPPISLLSDARTMAVYADINLFVLRIGYSTVKELSFINNTVEE
                     ETVRNMIVVLNDTPPSGKKIKKAAYFKD"
     gene            2314..3675
                     /locus_tag="VKU83_03540"
     CDS             2314..3675
                     /locus_tag="VKU83_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92649.1"
                     /translation="MLYLTKIKSQFFGGHARTAKANKHIVYSFFIKGLSIGCQFALVP
                     LTLHYLDSERYGIWLTMASIVGWFSFFDVGIGNGLRNKLSEALAKGDNRLAKIYISTS
                     YACVAAIFLTLMVLFWVVSPFLNWASILNTGQGLAGELGRMMLVVFSFFCLQFILNLI
                     GNILYAHQEPALTNLIMPLGNIVSLIIIYILTLTTHGSLFWVAVVFSASPVFVLLLFN
                     LFFLGWKFRAIAPGIRYVQFRHSRDLFGLGFQFFVIQIAFIVMYSTANILLIRWFSPA
                     TVTVYNIAYKYFTIALLANGIITATYWSAFTDAYVRQEFDWVRNTIRRMEKVTYLLMG
                     MVVLQTLLAGPLIGLWLHNAIQVPLSMQITMCVYVVISLIAAPQHIFLNGTGKIRLQL
                     YTAIYTIFMDIPFAWVFCKVLHLGPKGVILAMICTSLPVTILYRIQYNKIMSGNATGI
                     WVK"
     gene            3663..>4449
                     /locus_tag="VKU83_03545"
     CDS             3663..>4449
                     /locus_tag="VKU83_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92650.1"
                     /translation="MGKVTIGKILSYKFYSLLGRCCRLAFNLSLPRRLAPDKPLGETD
                     LLLFSGRKGARMMKATLLSIYYNWDRVPRLTIVSDGSPKEVFEAALRFWPYPYEIKSW
                     EDCASWHRQRGRKAIIDFAHVNPYARKLLSLLAEAERRPVFYCDTDVLWFAEPRLPSS
                     RSPASRSDVGPGGRGGATARLPDAEAEGAPKGGPCVMRISRDHEHCYHLPAIRYLNRE
                     DLLAKPAINCGVVYLSGSVYDNYPGFESLMEFMRIFSEPFSEQT"
BASE COUNT          987 a         1388 c         1109 g          965 t
ORIGIN      
        1 cggcaggcag gttttcacac cgggaaaagc ttcaataaga gaatgatcct gcccgcgatc
       61 ctccgcaact ggtactggtt cctcctggcg ggcgctgtcg ggttcggcgt ggcttttgtt
      121 cttcgcaagg tatttccggg cagctaccgg agcacaatga ctatcctgct catcaattcc
      181 ccacgccagt cgcctttcac ctcttccctt gacaataacc tcgaaatcca ggaaaacccc
      241 attaatgtcc aggacgaaca aacggttcta tcggcataca gcctgcagct ccagacgttg
      301 cagaacctcg gttggaaaac ccgctggtac aaaaagtcga tcttcggtcg caaggacatt
      361 tacaaagagg acccattcgc ggtcgaattt atcgaggact ccgtcctgaa aggcgtcgac
      421 ctgctcatca cgccgctttc caccacccgt taccgggtcc agtgcgaccg gaaggaccgc
      481 gtcggcgata ccgatcgcgt catccggttc aataccgtcg cggaattcgg gaagccattc
      541 aacaatgagt ggttccattt tacgctctat cccgctacgc cgccggttcc ggccaacggc
      601 gcccaataca tcctggactt caacgacctt tatttcatgg ccatcgaata ccagggcaac
      661 ctcgaagtga agatcggtgc gccggaaagt gatgtcctca atgtcgagct gaaaggcgag
      721 aatgtgcttc ggaacgtcga ttacctcaac gaactcggca aaacctacca aaaattcggc
      781 ctggaccaga agaaccagtc agcgatcaac acgcttactt tcatccggaa cctgatctcg
      841 ggcgtggccg actcgcttcg cacggccggg gaccgttata cggatttccg tacccgcaac
      901 cgggtagtcg atctcagcac ggagagttcc atggtcctgc aaaaagtaga agaccttgat
      961 aagcaggaaa gcctgctcaa gcttaagctg gactatttca acgggctgac ccgccacctg
     1021 aacgacggcg acagcctgaa gagcttcgtc gccccgccgc tcggcgatcc ggatcccgac
     1081 ctcagcgcca acgttcagaa actggcttcc ctgtacagcc agcggacggc cttgtctttc
     1141 tccgtccagc ccaaaaaccc ccggatgatt gccatcaacg acgatatcag cctcacccaa
     1201 cggctgatcc agggtaatat cgcgagccac ctcaccgcca tccgcgacca gatcagcgcc
     1261 ctcgaacaac aaaaagcaga aacgaatgaa aggttgacga atatcccggc aacggaacgt
     1321 tcctatttgg atatcaaacg gggatttgac gtcaacagca ccctgtacaa cttccttctc
     1381 cagaagcgcg ccgaagccgg catagccctg gcctcaaata acccggacgc caggatcctc
     1441 gatccggcga cattgcctac gaccggccct atcggattga aaccgatcgt cagtgcggcc
     1501 attggcgtca tcctaggcat gctggtcacc cttggcctgg tgctgctgaa ccaatatctg
     1561 aacgaccggc tccgcgaacc cgaagaagct gcagcggccc tgcacctgtc cgtggcaggc
     1621 cagatccccc acaaccgcct gaagacggaa ttgcccaccg tgcaccaccc gcgttcggaa
     1681 atcacggaat ccttccggaa cctccgcgca aacctgcggt atctgatgaa ggacagcacc
     1741 ggcggcgtca ttgccatcca ttctgcaagc tcgggggaag gaaaatcctt tattgcggcc
     1801 aatcttgctt cgctcctggc cctcggcagc aagaaagcgc tcctcctgca ggccgacaaa
     1861 cacaataccc agcttgaaac gctattcggc gctcaacccc gcgtggacct gacgcaatac
     1921 ctcgaaggca cggtttcctt ttgggaaatg cccgcgtcga cggaaatacc cgggctgagc
     1981 tatatccgcg ccagccaacc ggacggcccg ctttccgaac tgacggacac gccggccatg
     2041 gagaaattca tccgggaggc ccgcgcgggg ttcgatttca tcatcatcga taatccgccc
     2101 atcagcctcc tcagcgacgc gcgcaccatg gcggtctacg ccgacatcaa cctgttcgtg
     2161 ctgcgcatcg gctacagcac ggtcaaggaa ctttccttta tcaataatac cgtcgaggaa
     2221 gaaacggtac gaaatatgat cgtcgttttg aacgacacgc cgccaagcgg aaagaagata
     2281 aagaaggctg cctatttcaa agactaagta cccatgctgt accttaccaa gataaaaagc
     2341 cagttcttcg gcggtcacgc cagaacagcg aaggcaaata agcatatcgt ctattccttt
     2401 ttcataaagg gattgagcat cggatgccag ttcgcgctcg tgccccttac actgcactac
     2461 ctggacagcg agcggtacgg catctggctg accatggctt ccatcgtcgg ctggttcagc
     2521 tttttcgacg tgggcatcgg caacggcctg cgaaacaaac tgtcggaagc cctggcaaaa
     2581 ggcgataacc ggcttgcgaa aatctatatc agcacgtcct atgcctgcgt ggcggcgatc
     2641 ttcctcacgc tgatggtgtt gttctgggtc gtcagcccat tcctcaactg ggcgagcatc
     2701 ctgaataccg ggcaaggttt ggccggcgag ctgggaagga tgatgctggt ggtattctcg
     2761 tttttttgcc tgcagtttat cctgaacctg atcggcaata tcctctatgc acaccaggag
     2821 ccggcgctga ccaacctgat catgcctctg ggcaatatcg tttcccttat catcatctat
     2881 atcctaaccc ttaccacgca cggctccctg ttctgggtcg ccgtcgtctt cagcgcctcg
     2941 ccggtattcg tcctgctgct gttcaacctt tttttcctcg gctggaaatt ccgggccatc
     3001 gcgcccggca ttcgctatgt acagttccgc cactcccggg acctctttgg ccttggcttc
     3061 cagttcttcg ttatccagat cgccttcatc gtgatgtact cgactgccaa tatcctcctc
     3121 atccggtggt tcagcccggc gacggtgacg gtttataata tcgcctacaa atatttcacg
     3181 atcgccctgc tggcaaacgg cattattacg gctacttact ggtcggcatt tacagatgcc
     3241 tacgtccgcc aggaattcga ctgggttcgc aacacgatcc gccggatgga aaaagtaacc
     3301 taccttctga tgggaatggt cgtgctgcaa acgctgctgg cggggccatt gatcggcctc
     3361 tggctgcaca acgccattca ggtccccctc agcatgcaga tcaccatgtg cgtctatgtc
     3421 gtcatcagtc tcatcgcagc cccgcaacat atttttctga acggcacggg caagatccgg
     3481 ttgcaactgt atacggccat ttacacgatc tttatggata ttccctttgc gtgggtcttc
     3541 tgcaaggtcc tgcacctcgg cccgaaagga gtcatcctgg caatgatctg taccagcctg
     3601 cccgtaacca tcctgtaccg catccaatat aataaaatca tgagcggcaa cgctaccgga
     3661 atatgggtaa agtaacgatt ggcaagatcc tctcttataa gttttatagc ctgctgggcc
     3721 gttgctgccg ccttgccttc aaccttagct tgccccgaag gcttgctccc gataagcccc
     3781 tcggcgaaac cgacctcctg cttttttccg gccgtaaagg tgcaaggatg atgaaggcca
     3841 cactcctttc catatattat aactgggaca gggtgccccg gctcacgatc gtttccgacg
     3901 ggtcgccgaa ggaggtcttt gaagccgcgc tgcgtttctg gccctacccc tatgagatca
     3961 aatcatggga ggattgcgcc tcctggcacc ggcagcgcgg acggaaggcg atcattgact
     4021 ttgcccacgt caacccctat gcccgcaaac tgctgtcgct gctggccgaa gcggagcggc
     4081 gacccgtgtt ctattgcgac accgacgtcc tctggttcgc cgaaccgagg cttccctcat
     4141 cccgctcccc cgcttcacgc agcgatgtcg ggcccggagg tcgcggcggg gcgacagccc
     4201 gcctgccaga tgcagaagcc gagggtgcac caaaaggcgg cccttgtgtc atgcgcatct
     4261 cccgcgacca tgagcattgc tatcaccttc cggctatccg ctacctcaac cgcgaagacc
     4321 tgttggcaaa acccgccatt aactgtggag tcgtttacct ctccgggtcg gtctatgaca
     4381 actatccggg attcgagtcg ctgatggaat ttatgcgcat tttcagcgag cccttttccg
     4441 agcagacca
//
LOCUS       DATGKE010000203         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1138985, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000203 DATGKE010000000
VERSION     DATGKE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138985"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1118
                     /locus_tag="VKU83_03550"
     CDS             135..1118
                     /locus_tag="VKU83_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLI92651.1"
                     /translation="MNKGIKIIVNYVLGPLIFLVLCVFIYWQITRQHNWRTSLHQVLL
                     ALSGPNQWRLWLVLGLMPVNWGIEARKWQLALRPIGGLPFGRAFRAIFTGAAVASFTP
                     NRMGEYLGRILYIKEGHRTQGIALTIACSMAQLMITLVLGLAGIFYLSTAAHRASQSN
                     FQLVLNILIGIDLLILISLTFIYFRLSWVADLLLKIPGARKFASYINVLENFDTAILL
                     RILFLSFGRYIVFIAQYSAVFPVFGVDIGFWETGGGMSVVFLIMAVVPTFTILTELGL
                     RWEASIQIMELFSPNGVGIFAASFAIWLINLIIPALIGSLFIVSIKLFKNR"
     gene            1115..1966
                     /locus_tag="VKU83_03555"
     CDS             1115..1966
                     /locus_tag="VKU83_03555"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HLI92652.1"
                     /translation="MRVITWVCIMLMIFFPVITWVANAQQKDTDTVVQQPTQPEDEFC
                     RNLHNTAFKAGESITYKVYYTLAGVYAAAGECTFNTALEHFQGKDVYHVIAEGKTYPF
                     YDKFFRVRDKYESYIDTGNLQPYKFIRDVDEGGYKTYENVTFVKAAHTAITNSGVYNV
                     PECIQDVLSATFYARNIDFSHHKPGDKIPFAMFLDRQVYHLYVRYLGKETIRTKYAKF
                     RTIKFKPLLVKGTMFEGGEKMTVWVTDDPNHIAVRIESPITVGKVSVDMFTYRNPRSP
                     LTSLVDL"
     gene            1977..2477
                     /locus_tag="VKU83_03560"
     CDS             1977..2477
                     /locus_tag="VKU83_03560"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HLI92653.1"
                     /translation="MNLTALVKVFLIATLIPGWLTYTDSANKFTMNYPREWTQKASGN
                     IVAFLSPKADANDQFQENVNLMLQDISAQPMTLEQYTDLTKKQVTDAGGAGAIVSQGT
                     KTIGGHKAIQLIYNFSYQGHALKVEQYFFIKGKTVFLFSYTAEPGQFVKYEQTAIAII
                     NSFTFL"
BASE COUNT          620 a          654 c          602 g          649 t
ORIGIN      
        1 tgtccttgga agtaggtagc cgcaggacgc ccatttcgat aagcgtaagt ctgtccccgg
       61 cgcttttgat caatccataa cccatgacaa ccgtgccggg gtcgatacct aaaattattt
      121 tagattgcat tgcgttgaat aaaggtatta aaataatagt caactacgta ttagggccgc
      181 tcatcttcct tgtgctctgt gttttcattt actggcagat cacccggcaa cataattgga
      241 gaacgtccct gcaccaggtg ctgttggccc tctcaggtcc gaaccaatgg agactttggc
      301 tggtgctcgg cctgatgccg gtaaattggg gcatcgaggc gcgcaaatgg cagctggcgc
      361 tacgcccgat cggcggcctg ccgtttggga gggccttccg ggccatattc accggggccg
      421 ccgtcgccag ttttaccccc aaccgcatgg gggaatacct gggccgcatc ctgtacataa
      481 aggaggggca tcgtacccag ggcatcgcgc tgaccattgc gtgtagtatg gcacagctga
      541 tgatcacact ggtccttggc ctggccggta tcttctatct ctcgaccgcg gcccatcggg
      601 caagccagtc aaattttcag ttggtgttga atatcctgat aggaatagac ctgctcatat
      661 taataagttt aacatttatt tattttcggt tatcctgggt agcggatctg ttgctgaaga
      721 tccccggcgc ccgtaagttt gcgtcatata taaatgtttt agaaaacttt gatacggcca
      781 tcttattgag gattttgttc ttatcttttg gcagatatat tgtatttatt gcccaatata
      841 gtgcagtctt ccccgttttt ggggtagata tcggtttttg ggaaacaggg ggcggcatga
      901 gtgttgtatt tttgattatg gccgtcgtcc ccactttcac gatcctgacg gaattgggtt
      961 tgaggtggga agcgagtatc cagattatgg aactgttcag tcccaacgga gtggggatct
     1021 tcgccgcctc ctttgccata tggctgatca acctgatcat accggccttg ataggaagct
     1081 tgttcattgt aagtatcaaa ttattcaaaa atcgatgaga gttattactt gggtctgtat
     1141 catgctgatg attttttttc cggtaattac ctgggtagct aacgcccagc agaaggacac
     1201 cgataccgtg gtgcagcagc ccacccagcc cgaggatgaa ttttgccgta acctgcacaa
     1261 caccgctttc aaagcggggg aatccatcac gtataaagtt tactataccc ttgccggcgt
     1321 atacgccgcg gcaggtgaat gtacattcaa tactgcactc gaacatttcc agggtaagga
     1381 cgtctaccat gtgatcgccg aaggtaagac ctatcccttc tatgacaagt tcttccgcgt
     1441 gcgggataaa tacgaaagct atatcgatac cggtaacctc caaccctaca agttcatccg
     1501 cgatgtggat gaaggcgggt acaagaccta tgagaacgtc acctttgtca aagccgccca
     1561 taccgctatc accaattccg gcgtatacaa cgtgccggaa tgcattcagg acgtgctgag
     1621 cgccactttc tatgcccgca atatcgactt ctcccatcac aagccgggcg ataagatccc
     1681 cttcgccatg ttcctggacc ggcaggtcta tcacctttat gttcgttatc tgggtaagga
     1741 gaccatccgc acaaaatacg cgaagttccg caccatcaaa ttcaagcccc tgctcgtgaa
     1801 gggcaccatg ttcgaaggcg gcgaaaaaat gacggtctgg gtgacagacg atcccaacca
     1861 catcgccgtc cgcatcgaaa gtcctatcac cgtgggtaag gtatcggtgg atatgttcac
     1921 ttaccgcaac ccgcgctctc cgctcacgtc gctggtagat ttataaacca aatactatga
     1981 acctaactgc ccttgttaaa gtatttctga tcgccaccct catccccggt tggctcacgt
     2041 acaccgacag cgccaacaag ttcacgatga actatcccag ggagtggacc caaaaagctt
     2101 ccggcaatat cgtagcattt ctaagcccga aagccgatgc caatgaccaa ttccaggaga
     2161 acgtgaatct tatgctgcag gacatttccg cacagccgat gacgctggag caatataccg
     2221 atcttaccaa aaaacaggtc acggatgcag ggggcgcggg tgccatcgtc tcccagggca
     2281 cgaagaccat cggcggccat aaggcgatac aattgatcta caacttcagc tatcaaggcc
     2341 acgccctgaa ggtcgagcaa tactttttta ttaaagggaa gaccgtcttc ctcttttcct
     2401 acaccgcaga gcccggccaa ttcgtcaaat acgaacaaac ggccatcgcc attataaata
     2461 gcttcacgtt cttgtgattt tttgtcgtaa cttgtaacgt aataggttac atttgcataa
     2521 tgatt
//
LOCUS       DATGKE010000204         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1165467, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000204 DATGKE010000000
VERSION     DATGKE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165467"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /locus_tag="VKU83_03565"
     CDS             <1..2051
                     /locus_tag="VKU83_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92654.1"
                     /translation="IAGDPHSALKDAHTVVITERMAEKYFDATPGTMSSVVGRTLTIN
                     DSIPYKVTGVIRNIPTESHFHFDFFLSLTESDEARRTDDAWLSSNFQTYVLVRQGTDP
                     RQLQAKLDAMAARHVGPLLQQVVHVSLEDFSKSGNKFAFYLTPLTAIHLRSNRIAELG
                     ANGSIQNVYIFSAIAVFILLIACVNFMNLSTARSANRAREVGIRKVMGSMRGGLVSQF
                     LLESVLISLIALVLAIGLAMLLLPVFNHLSGKEMAIGAFMRPWMWPTALGLVLVVGML
                     AGSYPAFFLSAFQPVQVLKGKIAGGMRTGWLRNTLVVFQFSISIFLLVGTAVVYGQLS
                     YIRSRDIGFNREQVMIINNTYVLGTQAKAFENQLKQLSGVEGATMTAAVPTGGYGNND
                     AIFLSPVMDVKKSISTQVWPVDDQYVPVLGMKILAGRNFSKDFGTDSSAVLINESAAA
                     LLQGEKPVGAKLYEIEDLQTKKVKEYHVIGVVRNFNFNSLREVVTPIMLYPGTDEGRI
                     SLRIREKNAHQLAGQIEKLWRRAVPSQPFSYTFMDDEFNNQYLSEQREGSIALSFSVL
                     AIFIACLGLFGLTAYAAEQRTREIGIRKVLGATVVGIISLLSRDFLMLVGIAMLIAFP
                     FSWWAMHQWLQEFAYRIGIGWEVYVLTAVVAVAIALLTVSFQAVRAALLNPVDSLRTE
                     "
     gene            2300..>2537
                     /locus_tag="VKU83_03570"
     CDS             2300..>2537
                     /locus_tag="VKU83_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92655.1"
                     /translation="MNKIFPNDSRSIIETVLVLLLLLLLMLALYDVLRVFFGVLTFAL
                     IFSVSFFGPYERLVRWLRNQRSLGAVIYSIVLIAI"
BASE COUNT          498 a          673 c          744 g          622 t
ORIGIN      
        1 tgatcgccgg agatccgcat tcggcactga aggatgcgca taccgtagtg atcaccgagc
       61 ggatggcgga aaaatatttc gacgcgaccc ccgggaccat gtcatcggtg gtgggaagga
      121 cgctgaccat caatgactcc atcccttaca aggtaacggg agtgatccgc aatatcccta
      181 cggagtcgca ctttcatttc gacttctttc tttccctgac cgagtccgac gaggcaagac
      241 ggaccgacga tgcctggtta agcagtaatt tccagaccta tgtcctggtc cggcaaggca
      301 cggatccccg tcagctgcag gcgaaactgg atgctatggc cgcgcgccat gtagggccgt
      361 tgctccaaca ggtggtgcac gtgtcgctcg aagacttttc aaaatccggc aataaattcg
      421 cattttacct cacgccactg actgccattc atttgcgcag caacagaata gcggagcttg
      481 gcgccaacgg cagcatccag aacgtgtaca tcttttcggc catcgcggtt ttcatcctgc
      541 tgatcgcctg cgtcaatttc atgaacctgt ccacggcgcg atccgctaac cgggcgaggg
      601 aggtcggcat ccgcaaggtc atgggttcga tgcgcggcgg attggtctcc cagttcctgc
      661 tcgagtcggt attgatcagc ctgatcgcac tggtgctggc gattggactt gccatgctgc
      721 tgttgccggt gttcaatcat ctgtcgggaa aagaaatggc catcggcgct tttatgcggc
      781 catggatgtg gcctacggcg ttggggctgg tgctggtggt ggggatgctg gcaggtagtt
      841 atccggcgtt ttttctttca gctttccaac cggtccaggt attaaaggga aaaattgccg
      901 gcggcatgcg gacgggctgg ctgcggaaca cgctcgttgt ttttcagttc agcatttcaa
      961 tcttcctgct ggtcgggacc gcggtggtct acggacaact tagctatatc cgctcgcggg
     1021 acatcggctt taaccgcgag caggtgatga tcatcaacaa tacgtatgtt ctgggcaccc
     1081 aggcaaaagc gtttgaaaac cagctaaagc agctgtcggg cgtggaaggt gcgacgatga
     1141 cggcggccgt gcccaccgga ggatatggaa ataacgatgc gatctttctt tctcccgtca
     1201 tggatgtgaa gaagtcgatc tctacgcagg tctggccggt ggacgaccag tatgtgcccg
     1261 tattgggaat gaagatcctc gcagggcgca atttttccaa agactttggg actgattctt
     1321 cggcggtcct catcaatgag tctgccgcag cccttttgca gggcgagaag cccgttggcg
     1381 ccaaactcta cgagatcgag gacctacaga ctaagaaggt taaagagtac catgtcattg
     1441 gagtcgtgcg caatttcaat ttcaactcac tgcgcgaggt tgtgacaccg atcatgctct
     1501 atccaggaac ggacgaaggc cggatatcgt tgcggatccg ggaaaagaat gcgcatcaac
     1561 ttgccgggca gattgagaag ttatggagac gggcagtgcc gtcgcaaccg ttcagctata
     1621 cgttcatgga tgatgaattc aataaccaat acctgagcga acagcgcgaa ggtagcattg
     1681 ccctgtcctt ttcggtgctg gcaatcttca ttgcctgtct cggtctcttc ggcctgacgg
     1741 cctatgccgc cgaacagcgg acgcgcgaga tcggcattcg caaggtcctc ggtgcgaccg
     1801 tggtgggcat catttccctg ttatcgcggg atttccttat gctcgttggc atcgccatgc
     1861 tgatcgcatt ccccttttca tggtgggcga tgcatcaatg gctgcaggag tttgcatacc
     1921 ggattggtat cggatgggag gtttatgttc tgacggccgt agtggccgtc gcaatcgccc
     1981 tgcttacggt gagtttccag gcggtccggg ccgctttgtt gaatcccgtc gacagcttgc
     2041 gaacggagtg attcgcctcc ggaccgcccg gtggctaatg attcgccttc ggcggatcgg
     2101 cctgcggccg atctgtggcc gccttgttga atcccgtcga cagcttgcga acggagtgat
     2161 tcgcctccgg accgcccggt ggctagtgat tcgccttcgg cggatcggcc tccggccgat
     2221 cgaggggggg gcggggcgcg ccaaagcatc cccgggcaag gcacattttt cgctaacttt
     2281 agaagtaagc ggacgatcta tgaacaaaat ctttcccaac gacagccgga gcatcattga
     2341 aacggtactg gtgttgctgt tgttgctgct gttgatgttg gcgctttatg acgttttgcg
     2401 ggtatttttc ggcgtcctca cttttgccct gatcttttcg gtttctttct ttggtcccta
     2461 cgaacggctc gtccgctggc tgcgcaacca gcggtcgctg ggagcggtca tctattcgat
     2521 cgtgctgatc gctattt
//
LOCUS       DATGKE010000205         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_282406, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000205 DATGKE010000000
VERSION     DATGKE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282406"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..774
                     /locus_tag="VKU83_03575"
     CDS             214..774
                     /locus_tag="VKU83_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLI92656.1"
                     /translation="MNILSDNALMLKVKSGDLDKMSLLFQRYHRPLYGFLYHMTGQRE
                     SSEDMVQTVFYRLLKSRHTFTGEGEFKTWMYHVARNVLSDHFKGSRRAGYFADVAGLE
                     EKLATDGALFDRVEKRLAVQALHKAMAALSPESREILVLSRFQELKGAEIAGILEISV
                     GAVKVRIHRAISQLKAHYLQQQTEIE"
     gene            776..1744
                     /locus_tag="VKU83_03580"
     CDS             776..1744
                     /locus_tag="VKU83_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HLI92657.1"
                     /translation="MSCQKHKELLERWWNDQLPPGERADLAAQLADCEDCRRELEGSR
                     ELWDLMGYMPVPEPSGEMQLQFNAMLETYKASEEGRRASAGGWPGFWAVFASRPGISV
                     AFSLLMLLAGAGIGWLLRPAGTASAGSRQDAAEKRVIRDTVYIAQAPPREVLPTKNIE
                     SGGREDNAVAAAGVGRRQSESRQLEALAAQVHEMRELMMLSLLQNPAASERIKAVSYT
                     SEMRHADPNIASALLATLDNDPNVNVRLTTLEALTHYADDPAIREGLVQSILQQDSPL
                     VQVALADVMLKLQEKRAISPFRKLLQQKDLNETVRTKIQQTIVRLT"
     gene            1757..>2065
                     /locus_tag="VKU83_03585"
     CDS             1757..>2065
                     /locus_tag="VKU83_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92658.1"
                     /translation="MKKLFFPLMAGLGLICCFRTVALAQQQEYKEHISKAFTVQKTSG
                     GVLALYNLNGSLNVEGYAGDKVLIEVDERLTADNSEALEAGKKEFRLGLEQSGDSIIV
                     Y"
BASE COUNT          494 a          503 c          672 g          396 t
ORIGIN      
        1 ggctcagggg cgcggaagga ccttgggaaa atccgggaag gccgatggag gcggttagca
       61 agacgacaag caagttcctc atggcgacag aaattatttc gccggaagat agactttttg
      121 ccggagataa aataaatctt agattttcgg taaccttttc ccgccggcgc agtttctctt
      181 accggtccat cgaacaatat acaaccgcgt tgcttgaata ttctatcgga taatgccctc
      241 atgctgaaag tgaaatccgg ggatctggat aagatgtcgt tgttattcca gcgataccac
      301 cggcccctgt atgggtttct ctatcatatg acggggcagc gcgagtcgag tgaagacatg
      361 gtgcagacgg tgttttatcg cctcctgaag tcaaggcata cgtttaccgg ggaaggcgaa
      421 ttcaagacct ggatgtatca tgttgcccgg aacgtattga gcgatcattt caagggcagc
      481 cggcgggcag ggtactttgc ggatgttgcg ggcctggagg aaaagcttgc gacggacggc
      541 gccctctttg accgcgtgga aaaaaggctg gcggttcagg ccctgcataa ggcgatggcc
      601 gcgctgagcc ccgaaagccg cgagatcctc gtcttaagcc ggttccagga actgaaaggc
      661 gcagagatcg cgggcatcct cgagatcagt gtcggagcag tgaaagtgag gatccaccgg
      721 gccatcagcc aattgaaggc gcactattta caacagcaaa cggaaatcga ataatatgtc
      781 gtgtcagaaa cataaagaat tgctggagcg ctggtggaat gaccagctgc cgcccgggga
      841 gcgggcagac ctggcggcgc agctggcgga ttgtgaagac tgcaggcggg agctggaagg
      901 cagccgggag ttgtgggatc tgatgggcta tatgccggta ccggagccat ccggtgagat
      961 gcagctgcag ttcaacgcca tgctggagac ctataaggca tcggaagagg gaaggcgggc
     1021 gagcgccggc ggctggccgg gtttttgggc cgtattcgcg tcgaggccgg ggatctccgt
     1081 ggcgttcagc ctgctcatgc tcctggccgg cgcaggaata ggctggttgc tgagaccggc
     1141 ggggactgca agcgcagggt cgcggcagga cgccgctgaa aagcgggtga tcagggacac
     1201 ggtttatatc gctcaggcgc cgccgcggga agtgctgcca actaaaaata tcgaatcggg
     1261 aggccgggaa gacaacgcgg tcgctgccgc aggagtggga cgcagacaat ccgaaagcag
     1321 gcagctcgag gcgctggcgg cgcaggtcca cgaaatgcgg gaattgatga tgctgtcttt
     1381 gctgcaaaat ccggcggcgt cggagcggat caaggcggtg agctatacca gcgagatgcg
     1441 gcacgcggac ccgaatattg catcggcctt gctggcgacg ctggataatg atcccaatgt
     1501 gaatgtgcgg ctgaccaccc tggaggcgct gacgcattat gccgatgatc ccgccatccg
     1561 ggaagggctg gtgcaatcca ttctccagca ggattcgccc ctggttcagg tggcgctggc
     1621 ggacgtcatg ttgaaactgc aggaaaaacg ggcgatcagc ccattcagga agcttttgca
     1681 acaaaaagat ctcaatgaga cggtgcggac taaaatccag caaaccatcg tccggctaac
     1741 ttaaaaacaa aatgatatga aaaagctgtt tttcccgctg atggcgggac tgggattgat
     1801 ctgctgcttc cggaccgttg cgctggccca gcagcaggaa tacaaggagc atatcagtaa
     1861 agcgtttacg gtccagaaga cctcgggcgg cgtcctggcg ttgtacaacc tgaacggatc
     1921 gctcaatgtc gaaggttatg ccggcgacaa ggtgctgatc gaagtcgacg agcggcttac
     1981 agccgacaac agcgaagcgt tggaggcggg gaaaaaagag ttcaggctgg gcttagaaca
     2041 gagcggcgac agcatcatcg tgtat
//
LOCUS       DATGKE010000206         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1063943, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000206 DATGKE010000000
VERSION     DATGKE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063943"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VKU83_03590"
     CDS             <1..485
                     /locus_tag="VKU83_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92659.1"
                     /translation="KRTYGWVFAFLSVAFIGLSQLNALLLRRFKSEQIVRLALAGQVI
                     VAAFFWLADAAGWLGLGPTILFLFVFLACLGFTNPNAAALSLAPFSKNAGTASALLGF
                     FQLGIGTLASVAVSVFSTGTASPMVAVIAISSVLAMVILLAGHGSLATRPSGNDFSTR
                     "
     gene            complement(434..1162)
                     /locus_tag="VKU83_03595"
     CDS             complement(434..1162)
                     /locus_tag="VKU83_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI92660.1"
                     /translation="MTAAKNVLVLGANSDVAKECIKLYLAKGYTVTAASRGTDALRAF
                     ASANGILQGRLDIRYFDATAFGEHEAFYTALPVKPHIVLYAAGFQVTNEQALTDWEGS
                     YRMIQVLYAGAVSMLNRVVMDNRNQNLQRIIGLSSLSGVRGRKSNFIYGSTKSAFTQY
                     LAGLRQYLFPRQVIVNAIVAGYIRTKLTAGLDLPESLLLEPSFVAEAVVNAGNRFIIV
                     PGWKWKLIYRVLKSLPEGLVAKLP"
     gene            1523..1957
                     /locus_tag="VKU83_03600"
     CDS             1523..1957
                     /locus_tag="VKU83_03600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HLI92661.1"
                     /translation="MSIADRLFNRNNEKSAANLKAGQDFLEQNRKKPGVVALPSGLQY
                     EVLVEGKGLKPVATNKVTCHYHGTLIDGTVFDSSVRRAQPATFPLNGVIKGWTEGVQL
                     MPIGSKWRFYIPPHLAYGDRQVGAQIGPNSTLIFDVELISFS"
     gene            complement(1950..2417)
                     /locus_tag="VKU83_03605"
     CDS             complement(1950..2417)
                     /locus_tag="VKU83_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HLI92662.1"
                     /translation="MKRSTRRNFIGAGAAAAGAVLATTSVLGTAAAPGTTASPAHMLP
                     AASRPPVIHHVFFWLKNPSSIEDRDKLVAGLKTLAGIPLIKELYVGVLAATEKRDVVD
                     ASWQVSELMFFHDLQGQASYQQHPLHLDFVKNCSPLWEKVVVYDAMNVYAHIS"
BASE COUNT          542 a          743 c          826 g          582 t
ORIGIN      
        1 ataagcgcac ctacgggtgg gtgttcgctt tcctgtccgt ggctttcatc gggctgagcc
       61 agctgaacgc cctgttgctg agaaggttca aaagcgaaca gattgtgcgc ctcgcgctgg
      121 cgggccaggt gatcgtggcc gcgttttttt ggctggcgga tgccgccgga tggctgggcc
      181 tgggcccgac gatccttttc ctatttgttt ttctggcctg cctgggtttc accaatccga
      241 atgccgcggc cttgtcgctg gcgcctttca gcaagaatgc gggaaccgcg tctgccttgc
      301 tcgggttctt tcaattgggg atcgggacgc tggcatccgt ggcggtgagc gtatttagca
      361 ccggcacggc gtccccgatg gtggccgtga tcgcgataag ctctgtgctg gcgatggtta
      421 tcctgctcgc gggtcacgga agtttagcga cgagaccctc gggcaacgac tttagtaccc
      481 ggtagataag cttccatttc cagccgggga cgatgataaa gcggttgccg gcatttacca
      541 cggcctccgc gacaaaagac ggttcgagca gcagcgactc gggcaagtcg aggccggcgg
      601 tcagtttcgt ccggatatag ccggcgacga tggcgttgac gatgacctgc cgggggaaca
      661 gatattgacg gaggcctgcg agatattggg taaaggccga cttcgtgctt ccatagatga
      721 agttgctttt tctgccgcgg acgccggaga gcgaggacag gccgatgatc cgttggaggt
      781 tttggttccg gttgtccatg acaacgcggt tcagcataga taccgcgccg gcataaagga
      841 cctggatcat ccgatagctg ccttcccagt ccgtgagcgc ctgttcattg gtgacctgga
      901 aacccgctgc gtataaaacg atatgcggtt tgacgggaag tgcggtgtaa aaagcctcgt
      961 gttctccgaa cgccgtggca tcgaagtacc ggatatcgag ccggccttgc agaatgccat
     1021 tcgctgaagc gaatgcccga agtgcgtccg tgccgcggga ggccgcggtt acggtatatc
     1081 cttttgccag gtataatttg atacattcct tagccacgtc ggaattcgcg cccaggacga
     1141 ggacgttctt cgctgctgtc atgccataaa attagttagg attggctatt cccagcgacg
     1201 gtaaatcctg taagtttcaa atacgcgcat cgcaggatcg ccgtagatgg gcgggcagtc
     1261 aagattggcg ctgccgatgt gaagccggcg ctgctgcccc ccgaccatcg tcccagttaa
     1321 ttgggccgaa atcggcccaa ttaagtccaa cacgtccaaa ggcccgcccg ctccgccgcc
     1381 ccgcccgctc cgtcgccccg cccgctccgc cggcccggcc gcccggccgc cccgcccgaa
     1441 attgcgactc ccggtcctca gctgccatca cgcggctggc aggatttggc ggtaatgcgg
     1501 tatttttgca aaaaatgatc ctatgagtat tgccgatcgg ctgttcaaca ggaataatga
     1561 aaagtccgcc gccaacctga aggccggcca ggatttcctt gagcagaaca ggaagaaacc
     1621 gggcgtcgtg gctttgccaa gcgggctgca gtacgaggtc ctcgtggaag gcaaggggct
     1681 gaagcccgtt gcaacgaata aagtgacctg ccattatcac gggaccctga tagacgggac
     1741 ggttttcgac agctcggtca ggcgtgcgca gccggcgaca ttcccgctga acggggtgat
     1801 caagggctgg accgagggtg tgcagctgat gcccatcggc agcaagtggc gcttctatat
     1861 cccgccgcat ctggcgtatg gcgacaggca ggttggtgcg caaatcgggc caaacagcac
     1921 cttgatattt gacgtggagc tgatctcttt cagctgatgt gggcatagac gttcatggcg
     1981 tcgtacacaa ccactttttc ccaaagcggg ctgcaattct ttacgaaatc gagatggagg
     2041 ggatgctgct gataggacgc ctgtccctgg agatcgtgga agaacatgag ttccgagacc
     2101 tgccaggagg cgtctacgac gtctcttttc tctgttgcgg caaggacccc gacgtacaat
     2161 tccttaatga gcgggattcc tgcaagggtc ttcaggcctg cgaccagctt gtcccggtcc
     2221 tcaatggatg acgggttctt cagccagaaa aagacgtgat ggatgaccgg cgggcgggag
     2281 gcagccggga gcatgtgtgc cgggcttgcg gttgtgccag gcgcagcggc tgttccgagg
     2341 acagaagtgg tcgccaggac tgctccggct gccgcggccc cggcgccgat aaagtttctt
     2401 cttgtcgagc gtttcatggc ggtaaagata ccaaaatgag gtggaaccgc gttggaccgc
     2461 aaacaaaaag gccgccagaa accgggcggc ctttttaagt tttatggagt aagcccccta
     2521 ctgcaccgag aatgcattga tctcaacccc aaaattcgca ctcaccgttc cggtagaggc
     2581 ggatgtgatc attaacggaa tggcatagct cgcattcgga tccggagtgg aattaccgtt
     2641 caccgttacc cgcgcatacg cctgggtctg ccctttctta atggtgaccg aag
//
LOCUS       DATGKE010000207         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_544544, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000207 DATGKE010000000
VERSION     DATGKE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544544"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..930
                     /locus_tag="VKU83_03610"
     CDS             55..930
                     /locus_tag="VKU83_03610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLI92663.1"
                     /translation="MLKSMTGFGRAEQTVGDKTFLVDVKSLNGKQFELQLKLPGFLKP
                     FEFSIRKILSEKLGRGSLDCTISLKQTGSAKPVTINTDLAKAYYHSLAGLSEELRLDP
                     SNILSTLLKLPEVITPTGDTLTDEEWQEFEKVILAAIDDLNGHRLNEGASLEEDLLLR
                     IDNILRHQAEVVRLEPRRQVKIKEGLRKLLDEQVGKENYDGNRLEQELIYYIEKIDIS
                     EEQVRLKNHCDYFRAVLMEPEENKGKKLSFILQEIGREINTTGSKAYDATIQQCVVLM
                     KDELEKAKEQVLNVL"
     gene            965..1447
                     /locus_tag="VKU83_03615"
     CDS             965..1447
                     /locus_tag="VKU83_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HLI92664.1"
                     /translation="MKNYPRLLVVACCFLGSGCSFPANVFEKDVPLPGQEWASSNQPT
                     FTFDISNRDTADLYNVYVVLRHTDAYDFNNIWIRGSAREPGDTAVHSQRYDLSLADNE
                     KGWTGVGMDDIYEHRIEIQHRTKFNRPGAYSFTLEQIMREDPLKHVLDVGVRIEKVPE
                     "
     gene            1513..2478
                     /locus_tag="VKU83_03620"
     CDS             1513..2478
                     /locus_tag="VKU83_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92665.1"
                     /translation="MPFKRKRLAIATAAYWFLLAYIIAGLAWWFIALETTNRQLARNQ
                     LEQLRRDDPAFQTRRAAILAEEKTQSAAYIGEGSTFLFIILLGGLFVYREVRHQIRFQ
                     QQQQNFMMAVTHELKTPIAVTKLNLETLRKFRLEEEKQQRIILATIQESNRLDTLANN
                     ILVASQLEGGGYIRTKEVVDFSALADRLVRDFRNRFPDREWEAQIQPDCTVSGDPFLL
                     QLLVSNLLENALKYSPREAPVKLSLEKRERHTLLAVSDRGPGIPDEEKRKIFAKFYRS
                     GQEATRQTKGTGLGLYLCRKIAADHRATIRVADNSPTGSIFTVEF"
     gene            2481..3182
                     /locus_tag="VKU83_03625"
     CDS             2481..3182
                     /locus_tag="VKU83_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92666.1"
                     /translation="MSAKISILLVEDEENLHEALKLNLELEGYEITSAYDGSGALRAV
                     QTEYFDLIILDVMLPGMDGISVTETIRVQNNDVPILILSAKNASADRVLGLRKGADDY
                     LTKPFNLEELLLRIQKLIDKNKKMHDKDSLGEQYSFGKNSIDFKAQEAVTKDGQRIQL
                     SKKEVMLLKLLIENRNEVVTREKILQAVWGYNVYPTTRTIDNFVLNFRKYFEEDSRHP
                     KYFHSVRGVGYKYTE"
     gene            complement(3191..>4547)
                     /locus_tag="VKU83_03630"
     CDS             complement(3191..>4547)
                     /locus_tag="VKU83_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HLI92667.1"
                     /translation="VDRNTCYVGEPIVATYKLYSRLNAESRVTKRPSLNGFSVYDMID
                     PSMDAVSVERVNGKPFTVHTIRKTQLIPLQAGSIDLDPVEVENSVHFVKVGPQQPLHH
                     SGNRMQDLLDQLSAEGNFGPEVEENVTLDTKPLTITVKPLPEAGKPADFNGAVGEFSV
                     EASLANPRVAAQDEATLHVVVKGKGNLPIITAPTVNWPSGIDAYDPVAKEDVNKTVVP
                     MSGAKSFDYTFTARNSGEYTIPAINFSYFDPVSQRYKTANSEPLDLQVSKAARRAAGY
                     APPPASPAAAAGKPEGFFRNVLEMVMAILICLGLLMGWRSLNKKDPKPAGKTKPGPGA
                     QHPASVSPPSYVPLEPAPDLLFDARQYFNSGDYRAFYREVNRAIWKAVSGKLDLPSSE
                     LNKHNVVTQLELRGWDAPSILSLENTLNECELNLYTPAYDRYNMQQLLRQAEWVIDRL
                     A"
BASE COUNT         1095 a         1277 c         1229 g          946 t
ORIGIN      
        1 tgggggaccg gtggccgtcc ccggcattaa cgaactatac aaaaagaaga cactatgctc
       61 aaatcgatga ccggtttcgg tcgggctgaa caaaccgtag gcgataaaac cttcctggta
      121 gatgtcaaat cgctgaacgg caagcaattc gagttacagc tgaaactgcc cggtttcctc
      181 aagccctttg aattctcgat ccgcaagatc ctctccgaga aactaggccg gggttcgctg
      241 gattgcacga tcagcctgaa acaaaccggc agcgccaagc cggtcacgat caatacggat
      301 ctggcgaagg cttattatca ttcgcttgcc ggtttgtcgg aagaactgag actggaccct
      361 tccaatatcc tcagcacgct tttgaaactg cctgaggtca tcaccccaac cggcgacacg
      421 ctgacggacg aggaatggca ggagtttgaa aaagtcatcc tggccgccat cgacgacctc
      481 aatggccacc ggctgaacga aggggcctcg ctcgaagaag acctgttgct ccgtatcgac
      541 aatatccttc gccaccaggc ggaagtcgtc aggttggagc cgcgccggca ggtcaagatc
      601 aaggaagggt tgcggaaact gcttgacgaa caggtcggga aagaaaacta cgacggcaac
      661 cgtcttgaac aggaactcat ttactacatc gaaaagatcg atatcagcga ggaacaggtc
      721 cggttgaaaa atcactgtga ttatttccgg gccgttttaa tggagccgga agaaaacaaa
      781 gggaaaaaat tgtccttcat tctccaggag atcggtcgcg agatcaatac caccgggtcg
      841 aaagcttacg acgccaccat tcaacaatgt gtcgtcctga tgaaagacga gctggaaaaa
      901 gcaaaagaac aggttttgaa tgtattgtaa tggccctacc tttatatata aattctcttt
      961 ttcattgaaa aactatccca gattactcgt cgtggcctgc tgttttctag ggtcgggctg
     1021 ttcctttccc gctaatgttt tcgaaaagga cgtgcccctc ccgggtcagg agtgggcaag
     1081 cagcaaccag ccgaccttta cgttcgacat cagcaaccgg gacaccgccg atctgtataa
     1141 tgtttatgtt gtcctgcgcc acaccgatgc ctatgacttc aacaatatct ggatcagggg
     1201 cagcgcccgc gagccgggcg atactgcggt tcacagccag cgatacgatc tgtcccttgc
     1261 cgacaatgaa aaaggctgga ccggcgtcgg tatggacgat atctatgaac accgcatcga
     1321 gatccagcac cggacgaagt tcaaccgccc cggcgcttac tccttcaccc tcgaacagat
     1381 catgcgcgaa gacccgctga agcatgtcct ggacgtgggc gtccggatcg agaaagtccc
     1441 ggaataggat gatcagctaa gctttggcgg agacgcggca aacttattta aatcgcgtcg
     1501 tccgccgcat atatgccatt caaaagaaaa cggctggcga tagcgacggc cgcctactgg
     1561 ttcctgcttg cctatatcat tgccggcctg gcctggtggt tcattgcgct cgaaacgacg
     1621 aaccggcagc tggccaggaa ccagcttgaa cagcttcggc gcgacgatcc tgcgtttcaa
     1681 acccggaggg cagccatcct ggcggaagaa aaaacgcaga gcgccgctta catcggcgaa
     1741 ggcagcacct tcctttttat catcctcctc ggaggtcttt tcgtctaccg ggaagtgcgc
     1801 caccagatcc gtttccaaca acaacagcag aactttatga tggcggtcac gcacgaattg
     1861 aagacgccga tcgcagtcac gaaactgaac cttgaaacgc tgcgaaagtt ccggttggaa
     1921 gaagaaaaac aacaacggat aatccttgca accatccagg agagcaaccg gctggataca
     1981 ctcgccaaca atatcctggt cgcctcacag ctcgaagggg gcgggtatat ccggacaaaa
     2041 gaggtcgtgg atttttccgc cctcgccgac cgcctggtcc gcgactttcg caatcgcttc
     2101 ccggaccggg agtgggaagc gcagatccag ccggattgca ccgtcagcgg cgatccgttc
     2161 ttactccaac tgctcgtcag caacctcctg gaaaatgccc tgaaatattc tccaagggaa
     2221 gcccccgtga agctttcact ggaaaagagg gaacgccata ccttgctggc cgtgagcgac
     2281 cggggacctg gcatcccgga cgaagaaaaa aggaagatct tcgcgaagtt ctaccggagc
     2341 gggcaggagg cgacgcggca gacaaaaggg accggccttg gcttgtatct atgccggaaa
     2401 attgcagccg accaccgggc gaccatccgc gtggccgaca attccccgac cggcagtatt
     2461 tttacagtgg aattctgact atgagtgcaa aaatttccat tctcctggtg gaagacgagg
     2521 aaaatctcca cgaagccttg aaactcaacc tggagctcga gggctacgag atcacgtctg
     2581 cgtacgacgg gtcgggggca ttacgggctg ttcagacgga gtatttcgac ctcatcatcc
     2641 tcgacgtgat gctgccgggc atggatggga tcagtgtcac cgaaacgatc cgcgttcaga
     2701 acaacgacgt gcccatcctt atcctcagtg caaaaaatgc gagcgcggac cgggtcctgg
     2761 gtctgcggaa gggcgcggat gactacctga ccaagccgtt caacctggaa gagcttcttc
     2821 tccgcatcca gaaactgatc gataagaaca agaagatgca tgacaaggac tcgttgggtg
     2881 aacagtattc ttttgggaag aacagtattg actttaaagc ccaggaggcc gtaacgaagg
     2941 acggccagcg catccagctg agcaagaagg aagtgatgct gttgaaattg ctgatcgaaa
     3001 accggaacga agtggttacc cgcgaaaaga tccttcaggc ggtttgggga tataacgttt
     3061 atccgaccac ccggaccatc gataacttcg tgcttaattt ccgcaaatac ttcgaagaag
     3121 acagcaggca tccgaaatac ttccattccg tccggggcgt cggctataaa tatacagagt
     3181 agatgcctcg ctaggccagc ctgtctatta cccattccgc ctgccgcaac aactgctgca
     3241 tattgtacct gtcatacgcc ggcgtgtaaa gattcagctc acattcgttc aacgtatttt
     3301 ccagcgaaag gatcgacggc gcatcccagc cgcggagctc gagctgggta accacattgt
     3361 gcttgttcag ttcgctggat ggcaggtcca gcttccccga gaccgccttc cagatggcgc
     3421 ggttcacttc ccggtaaaac gcgcggtagt caccgctatt gaaatattgc cgggcgtcaa
     3481 acaataaatc cggcgcgggc tccaacggga cgtagctggg cggcgatacc gaggcggggt
     3541 gttgcgcgcc tggccccggc ttcgtttttc ccgccggctt cgggtccttt ttattcaggc
     3601 ttcgccatcc catcagcagt cccaggcaga tcaggatagc catcaccatt tccagtacat
     3661 tccggaaaaa gccttccggc tttccggcgg ccgcggccgg cgaagcgggc ggcggcgcgt
     3721 aaccggcggc gcgtcgggcg gccttactga cctgcagatc caacggctcg ctattggccg
     3781 tcttatagcg ctgggaaacc ggatcgaaat aggagaaatt gatcgccggt atcgtatact
     3841 ctccggaatt cctggcggtg aaggtatagt cgaatgactt tgccccgctc atcgggacaa
     3901 ccgtcttgtt gacgtcttcc ttcgccacgg ggtcataggc gtcgataccg gacggccagt
     3961 ttaccgtcgg agccgtgatg atcggcaggt tacccttccc cttcactacc acatgcagtg
     4021 tcgcttcatc ctgcgccgca accctgggat tggccagcga ggcttcgacg gagaattcac
     4081 cgacggcgcc gttgaaatcc gcgggcttgc cggcttccgg cagcggcttc accgtaatgg
     4141 taagcggttt ggtatccagc gtcacatttt cctcgacctc cggaccaaaa ttcccctccg
     4201 ccgataactg atcgagaaga tcctgcatgc ggttgccgct gtgatgcaac ggttgctgcg
     4261 ggccgacctt cacaaaatgc acggaattct ccacctccac cggatcgaga tcgatgctgc
     4321 cggcttgcag ggggatcaac tgcgtcttgc ggatcgtgtg aacggtgaaa ggcttgccat
     4381 tcaccctttc cacggagacg gcgtccatgc ttgggtcgat catatcatac acgctgaagc
     4441 cgttcagtga aggccgcttc gttacccgcg actccgcatt caggcgggaa tacaatttgt
     4501 aggtggcgac gatcggttcg cccacataac aggtgttccg gtccacc
//
LOCUS       DATGKE010000208         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_802325, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000208 DATGKE010000000
VERSION     DATGKE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802325"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..315)
                     /locus_tag="VKU83_03635"
     tRNA            complement(245..315)
                     /locus_tag="VKU83_03635"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(281..283),aa:Cys,seq:gca)
     gene            473..1351
                     /locus_tag="VKU83_03640"
     CDS             473..1351
                     /locus_tag="VKU83_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HLI92668.1"
                     /translation="MTSIAESEFILNPRGAVYHLDLRPEELATTVLTVGDPDRVERVS
                     KYFDRIEYRRQHREFVTHTGWLGKKRLTVISTGIGTDNIDIVLNELDALANIDFATRT
                     VKPHLTTLTVVRMGTTGALQKDIPPDSWVASTHGLGIDNLLNFYRLDNNEEEIELLHA
                     FSTQTQLHQRLAHPYISSGSASLLKHFVADFHHGITVTCPGFYGPQGRVLRAGLAQPE
                     LIDRLTDFSYGRHRIANFEMETSGIYGLGKVLGHSCLSLSAVVANRITRQFSSDGQAA
                     VDGLIQRTLGILEELG"
     gene            1374..2159
                     /gene="dapF"
                     /locus_tag="VKU83_03645"
     CDS             1374..2159
                     /gene="dapF"
                     /locus_tag="VKU83_03645"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLI92669.1"
                     /translation="MDTLHFFKYQGTGNDFIILDNRNWSYTSLTRERIRLLCDRRFGI
                     GADGLMLLNPHAGFDFEMKYFNADGGESSMCGNGGRCLVKFAYDQGVHKSEYNFVAID
                     GPHLAEIDDQGIVSLKMKNVPSIEERKGDFILDTGSPHYVQMVSEVMDIDVCQKGMDI
                     RYSSEYAREGINVNFVEQKRPDEIFVRTYERGVEDETLSCGTGVTAAALACYHNEVGY
                     NFVKVITRGGKLTVRFDRNENGSFENVWLSGPAVRVFEGSIEN"
     gene            2161..>2701
                     /locus_tag="VKU83_03650"
     CDS             2161..>2701
                     /locus_tag="VKU83_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HLI92670.1"
                     /translation="MIQFFKNINHQVVEVDQPGSGTWVNLLPPLKQEEFARLSAELDI
                     PLDFLTDSLDIDERARFDEEENVKLIVIKTPTQNNSFNDSDAYYITIPICIILTHTQI
                     VTVNSFENGAIKKFLNTFQNRHPDKKNMMVLKIFEKITQTFMEYLKEINQRRNLMEQK
                     LYDSNRNEELLELMKIQKSM"
BASE COUNT          681 a          694 c          727 g          599 t
ORIGIN      
        1 actgtcacgt acggttttgt gagagcttag ggctgaaatg ctctattcta ctcgactgac
       61 ggggccggcg aataaaacag actattcctg catcaaaacc gaacagggaa gaatgcttga
      121 aagggcaatc gggcttcgcc cgatctcttt catttgtcgc tgcgcgtccg caagcaagct
      181 tgctaccgct ccgctccaga taaaataacc gccatccggc ggttattgtt tcccctttca
      241 tttgaggttt cgagcggatt cgaaccgctg taaaaggttt tgcagacctc tgcctagcca
      301 ctcggccacg aaaccacttt ttgccttccg ggcggatcag gcgatttgcg acaagcgccg
      361 cgcggagcct ttttcgccgt gaaaatcggc cccgggagga ggggccgcat ttttttgagc
      421 agctgcgaaa ttaagataat tttgaacaag ttagaaaaaa atcgggcaat ttatgacctc
      481 tattgctgaa tctgaattta ttctcaaccc acggggcgca gtttaccatt tggaccttcg
      541 ccctgaagaa ttggccacca ccgtccttac cgtcggcgat ccggaccggg tggagcgggt
      601 cagcaagtat tttgaccgga tcgaataccg aaggcaacac cgggaatttg tgacccacac
      661 cggctggctg gggaaaaaaa gactaacggt aatttctacg ggcatcggga ccgataatat
      721 cgacatcgta ttgaacgaac tggatgcact ggcaaacatc gattttgcga ccaggacagt
      781 caaaccgcac ctgacgacac tgacggttgt ccgtatggga accacgggag cgctacagaa
      841 agatatcccg ccggacagct gggttgcctc gacacacgga ctgggcatcg acaacctgct
      901 gaacttctac cggcttgaca acaatgagga ggagatagaa ctgctgcatg ccttttcgac
      961 ccaaacacaa ttgcaccagc ggctggctca tccttatatc agcagcggca gcgcttcctt
     1021 attaaagcat ttcgtggcgg acttccatca tgggatcacg gtcacctgcc cgggctttta
     1081 cgggccgcag ggtcgggtgc tgcgggcggg cctcgcgcag ccggaactga tcgaccgcct
     1141 gacggatttt tcatatggcc gccaccggat tgccaatttc gaaatggaaa cgtccggtat
     1201 ctatggcttg ggaaaggtcc tgggccattc ttgtctgagc ctgagcgcgg tcgtggcgaa
     1261 ccggatcacc cggcaattct cgtcggatgg ccaggcggcg gtggacggat tgatacaacg
     1321 gacccttggt atcctggaag agttgggata aatgagctat ttttgacccc cgtatggata
     1381 cgttacattt tttcaaatac cagggcaccg gcaacgattt tattatcctt gacaacagga
     1441 actggagcta tacctcgctg accagggagc gcatccggct cttgtgcgac aggcgctttg
     1501 gaataggcgc cgatggcctg atgctgctga atccgcacgc cggttttgat tttgaaatga
     1561 aatatttcaa cgccgacggc ggagaaagct cgatgtgcgg caatggcggt cgctgcctgg
     1621 taaaattcgc ctatgaccag ggtgtccata agagtgaata taattttgtc gccatcgatg
     1681 gcccgcacct ggccgaaatc gacgatcagg gcatcgtcag cctcaagatg aagaacgtgc
     1741 cttcaattga ggagagaaaa ggcgatttca ttctggacac cggctcgcca cattacgtcc
     1801 aaatggtatc tgaggtcatg gacatcgacg tgtgccaaaa gggtatggac atccgttaca
     1861 gcagcgaata cgccagggaa ggaatcaacg tcaacttcgt ggaacaaaaa cggcctgacg
     1921 agattttcgt gcggacctac gaacggggtg tcgaggatga aaccctttcc tgcggaaccg
     1981 gcgtgacggc ggccgccctg gcctgttacc acaatgaggt tgggtacaat tttgtgaaag
     2041 tgattacccg gggcggaaag ctgacggtac gcttcgaccg caatgagaac ggatcgtttg
     2101 aaaatgtttg gctttccggg ccggcggtgc gcgtttttga aggttcgatc gagaattgag
     2161 atgatccagt tctttaagaa tatcaaccac caggtcgtgg aggtcgacca gccaggcagc
     2221 ggcacctggg taaacctctt gcccccgcta aaacaagagg agttcgcgag gttatccgca
     2281 gagctcgata tcccgctcga ttttcttaca gactcgctcg atatcgacga gcgggcgcga
     2341 tttgatgaag aggagaatgt caagctcatc gttatcaaaa cgcccaccca gaacaactcc
     2401 ttcaacgata gtgacgccta ctacataacg attcccattt gcatcattct aacgcatacg
     2461 cagatcgtta cagtcaactc gttcgagaac ggcgcgatta aaaagttcct gaacaccttt
     2521 cagaaccggc acccggacaa gaagaacatg atggtcctga agatcttcga aaagatcacc
     2581 cagaccttca tggagtattt aaaagagatc aaccagcggc ggaacctcat ggaacagaaa
     2641 ttgtacgact ccaaccggaa cgaggagctg ctcgagctga tgaagatcca gaaatcgatg
     2701 g
//
LOCUS       DATGKE010000209         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1386092, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000209 DATGKE010000000
VERSION     DATGKE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386092"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VKU83_03655"
     CDS             <1..723
                     /locus_tag="VKU83_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLI92671.1"
                     /translation="KAQYAYYEPLHYAVRGGSLPMVELLLRHGAHPAAPGWSRLGDET
                     PLAKARYRRRNDMVALIEQAVATAPPYHWPPTRPFTPERQRRYDFEIACGYNPDLDLV
                     RRVLADHPGWATLGLYEAIHHDRMDITLLLLEAGGDPNGFMPFACWLTPLMHTLRYSQ
                     PRWQMAELLLDHGVPVRGTNGLGMTALHIVVAFGTVEAAKWLLEHGADPNAIEPEFSS
                     TPLGWAARWNRHDMKELLLAYY"
     gene            841..1386
                     /locus_tag="VKU83_03660"
     CDS             841..1386
                     /locus_tag="VKU83_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLI92672.1"
                     /translation="MSTTLSNPATAVGKNSKLQEFFVEQLQDIYWAEQKLVKTLPKMA
                     EAAHSHELQQAFEQHLRETENHVTRLERVFAAMGEKAKSKKCLAMAGIVDEGEDIIDD
                     TEENTAQRDVGLIFAGQKAEHYEIATYGGLVQLARTLGYEEAAREMSATLEEEKKADS
                     KLTSIGENSANPQAGREPKNK"
     gene            1615..2217
                     /locus_tag="VKU83_03665"
     CDS             1615..2217
                     /locus_tag="VKU83_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI92673.1"
                     /translation="MRVEGKFSDRELIAAIGGKKDLNNAIRFLYREYSRPVSSFLIGY
                     GASEQDADDVFQETVIAFIHIVQNGKYRMEASIKTFLVSVAKNVWRNEMKKRERTDFR
                     EQRFESGRAQSEEAVDEWIEESEKKRQLRDLVFQLGEPCRKLLMLFYYENLSMKEILT
                     HLPYGNEQVVRNKKYKCLQQLTEIIRQYPAIAGQLNELTK"
     gene            2224..>2851
                     /locus_tag="VKU83_03670"
     CDS             2224..>2851
                     /locus_tag="VKU83_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92674.1"
                     /translation="MELNREQLIDHLDSTRLGQGSAEMNARIAADPEASQQWKELCLA
                     LDAVEQAAIYQHVRNVKATIEAHPMKAVPAATSQSAIVRSVSRIALRAAAVILVLGAS
                     SVIYKYSVTSSSGIYSAFYTPYDLNTSRGAATAQDDLETAYKAHNWAGVDEAFRKKKV
                     KDNKACFLEGMADMEAKKYDDAIGMFQQVIAANSLSGADYFEDEAEFYL"
BASE COUNT          740 a          772 c          753 g          586 t
ORIGIN      
        1 aaagcccaat acgcctatta tgagccgttg cattacgccg tcagaggcgg cagtctgccc
       61 atggtcgagc ttctgctgcg ccacggcgcg caccctgcgg ctccgggttg gagcaggctg
      121 ggcgatgaaa cgccgctcgc gaaagccagg taccggcgcc ggaacgatat ggtagcactg
      181 atcgaacagg cggttgctac ggcgccgccc taccactggc cccccaccag accttttact
      241 cccgaacggc aacgccgtta cgatttcgag atcgcctgtg ggtacaatcc cgacctcgac
      301 ctcgtcaggc gcgttcttgc tgaccatccg ggttgggcaa ccctcggcct gtatgaagcg
      361 atccatcatg accgcatgga tatcaccctg ctactgctcg aggcaggcgg tgaccccaac
      421 ggtttcatgc cctttgcttg ctggctgaca ccgctgatgc acacgcttcg ctattcgcag
      481 ccccgctggc aaatggccga gctcttgctg gatcacggtg tgccggtccg gggaaccaat
      541 ggccttggca tgacggccct tcatatcgtc gtcgcatttg gcacagtcga agctgcaaaa
      601 tggctgctgg agcatggtgc cgaccccaat gccatcgaac cggaattttc ttccacgccg
      661 ctgggatggg cggcccggtg gaaccgccac gatatgaagg aattactatt agcgtattat
      721 tgaaccttcg cggccagcac aaggcgcgcc gcaacccgca atgaacccag cagccgtcct
      781 ctggcataat tatttaggca tccggaatga tattgaatct taaacttcaa aatcttttct
      841 atgtcaacga ctttatcaaa tccagccaca gctgtgggga agaactccaa gctgcaggaa
      901 tttttcgtcg aacaattgca ggacatctac tgggcggaac aaaaactcgt caaaacgctt
      961 cccaaaatgg ccgaagcggc ccactcgcat gaactgcagc aagcattcga gcagcacctc
     1021 cgcgaaacgg aaaaccacgt aacccgtctc gagcgtgtat tcgcagccat gggtgaaaaa
     1081 gcaaagtcca aaaaatgcct ggccatggcg ggtatcgtag atgaaggcga ggacatcatc
     1141 gacgacacgg aagaaaatac ggcccagcgt gacgtcgggc tgatctttgc aggccaaaaa
     1201 gccgaacatt acgaaattgc cacttatggc ggcctggtcc aactggccag aacgctgggc
     1261 tatgaagaag cggccaggga gatgtccgcg actcttgaag aagaaaagaa ggccgacagc
     1321 aagctgacga gcattggtga gaactccgcc aacccacaag ccggccggga acctaaaaac
     1381 aagtaaatca ccggcgtaac cggtccgagg atggcggggc cctgtcccgc catctttttt
     1441 tgcgcgcaat ctacccaaaa accaccaaaa aatgggctta ttgagccagg gatttttggg
     1501 cacataactc cctattatac aattggatgc ttcgattttc gttttaggta ggatcctata
     1561 tcaaaaaaaa cccggtagac cggttacatt ccctccctta gaacctacta ttatatgaga
     1621 gtcgaaggaa aatttagcga tcgagaactg atagcggcga tcggcggtaa gaaagacctg
     1681 aataatgcca tccggttcct gtatcgcgaa tattcccgac ccgtaagctc ttttttgatc
     1741 ggttacggcg ccagtgaaca ggatgccgac gacgtgtttc aggaaacggt catcgccttt
     1801 atccatattg tgcaaaacgg caagtatcgg atggaggcca gcattaagac cttcctcgtt
     1861 tcggtcgcaa agaatgtctg gcgcaacgaa atgaagaaaa gggagcgaac ggatttccgc
     1921 gaacagcggt ttgaatcggg ccgggcgcaa agcgaagaag cagtcgacga atggatcgag
     1981 gaatcagaga agaagcgtca gctgcgcgat ctggtcttcc aactgggtga accgtgccgg
     2041 aagttgctga tgctgttcta ctatgagaat ctgtccatga aagagatcct tacccacctg
     2101 ccctatggaa atgaacaggt ggtccggaat aagaaataca aatgtctgca gcaattgacg
     2161 gaaatcatca gacaatatcc ggcgatcgcg ggacaactga acgaactaac aaaatgaacc
     2221 accatggaac tgaaccgcga acaattaatt gatcatctcg acagtacccg ccttgggcaa
     2281 ggctctgcag aaatgaatgc gcggatcgcc gcggatccgg aggcgagcca gcaatggaag
     2341 gaactctgtc ttgcgttgga tgccgttgaa caggccgcca tctatcagca cgttcgtaat
     2401 gtgaaagcta ccattgaagc acatccgatg aaggctgtcc ctgccgccac aagccagagc
     2461 gctatcgttc gctctgtttc gcgcatcgcc ttacgggcgg ccgccgtgat cctggtcctc
     2521 ggtgcttcat cggtaatcta taaatactcg gtgaccagct cttccggaat ttattccgct
     2581 ttttacaccc cgtacgacct gaacaccagc cgtggcgctg ctaccgcgca ggatgacctc
     2641 gagacagcct ataaggccca taactgggca ggcgttgacg aagccttccg gaagaagaag
     2701 gtgaaagaca ataaagcctg cttccttgaa ggcatggcgg atatggaagc aaaaaaatat
     2761 gatgatgcaa tcgggatgtt ccagcaggtt attgcggcca atagcctttc gggcgccgat
     2821 tatttcgagg atgaagcaga attttacctc g
//
LOCUS       DATGKE010000210         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_439769, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000210 DATGKE010000000
VERSION     DATGKE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439769"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VKU83_03675"
     CDS             <1..1181
                     /locus_tag="VKU83_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLI92675.1"
                     /translation="RSCPMFDPTLVNLPTLRKLAYNLRWATLPPDTIPLTAADPDFPC
                     PAPVTEAISKFLTDRYFSYGPPEGSPAFKESMARYFRDRRHIPAEPASILPVDSAAFG
                     IYLTCKAFLAPGDEAIIFDPVDFLFRYAVEAADARAVTLPIPPGSPAAFDRLEELITP
                     RTRLICLCNPLNPTGKVFTKQELLDLGRLACRHDLLIMSDEIWSDIVFDPNKYHSIAA
                     LDADIRDRTITITGFSKSYGLAGLRIGAVMAGNPRLFSRLFNASLHQSTVHGANVISQ
                     VAATAALDECADWLRGFVIHLHRMRDLCVDSLNAIPGFTCRQPDGCYVAFADISGTGR
                     SAEEIREILFNRGKVAVVPGLPEWFGPGAANHIRLSFATSAAILQDAMTRITQTIRNL
                     "
     gene            1178..>2197
                     /locus_tag="VKU83_03680"
     CDS             1178..>2197
                     /locus_tag="VKU83_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLI92676.1"
                     /translation="MKYVVIGGGIAGLAIGAFLERKGREVVICEKQSGVTNQGHAFLL
                     HADAVSVLNELNLDPGRRLPGELIRHFSLRRPDGNEVRAETLEGWTCMKRTDLLEFMD
                     SLVQPSAVCRGRNFSHFLRENGRVVAAVFSNGTIEYGDIFIGCDGGKSKVRDAVFGSL
                     PATTIMVKEIVGIAKNSMLWPRHRNSFVKFQHDQYGLSFGMIPTGPDEFVWFMQFDPC
                     RGDISGIDHEQIRKFVIRSLSGFPSEVSELLRSNDFSRTYVWNTRDFDLLPAFHRDNI
                     VLVGDAAHQALPFSSSGTTNAIVGAAALSHALDNSLNLEAAFQQYYSQRAPVIREHVR
                     IGRELR"
BASE COUNT          423 a          707 c          609 g          458 t
ORIGIN      
        1 cgagatcatg tcccatgttt gacccgaccc tcgtgaatct tccgaccctc agaaaactag
       61 cctacaacct gcgttgggct accttacccc cggacactat tcccctgacc gcggcggacc
      121 ccgattttcc gtgccccgcc cccgtaaccg aagccatttc gaaatttctg accgatcgtt
      181 acttttctta cggcccgccc gaaggcagcc cggcttttaa ggaaagcatg gcgcggtact
      241 tccgcgaccg gcgccatatt cccgcggaac ccgcgtccat cctgccggtg gacagcgcgg
      301 ccttcggcat ttatctcacc tgcaaggcgt tcctggcgcc tggcgacgaa gccatcatct
      361 tcgacccggt tgattttctg ttccggtacg ccgtggaagc cgcggatgca agggcggtca
      421 ccctgcccat tccacccgga agtccagccg cgttcgaccg cctggaggag ctgatcaccc
      481 cgaggacccg ccttatctgc ctctgcaatc cgctgaaccc gacggggaag gtctttacga
      541 agcaggaatt gctcgatctc ggcaggctgg cctgccgcca tgacctgctc atcatgtcgg
      601 acgagatctg gagtgatatt gtttttgatc cgaacaaata ccatagcatt gccgcccttg
      661 acgccgatat ccgggaccgg accattacca ttaccggctt cagcaagtct tacggcctgg
      721 caggcctgcg gatcggcgct gtcatggcag gcaacccccg ccttttcagc cggctgttta
      781 atgcctccct gcaccagtcg acggtgcatg gggccaacgt tatttcgcag gtggccgcca
      841 cggcggcgct ggacgagtgt gccgattggc tgcgcgggtt tgtgatccac ctgcaccgga
      901 tgcgcgatct ctgcgtcgat agcctcaacg cgattcccgg ttttacctgc cggcagccgg
      961 acggctgcta tgtcgctttt gcggacatct ccggcacggg ccgaagcgcc gaagagatcc
     1021 gcgagatcct tttcaacagg ggaaaagtcg ccgtcgtacc gggcctgccc gagtggttcg
     1081 gacccggcgc tgcgaaccat atccggctga gttttgcgac gtcggcagcg atcctgcagg
     1141 acgccatgac gcgaataacc caaacaatcc gaaacttatg aaatacgtcg tcataggagg
     1201 tggcatcgca ggcctggcca taggcgcctt tctggagcga aagggcaggg aagtggtcat
     1261 ttgcgaaaag cagtccggtg tcaccaacca ggggcacgca tttctcctgc acgccgacgc
     1321 cgtgtcggtc ctgaacgagc tcaaccttga ccctggccgc aggcttcccg gtgaactgat
     1381 ccggcatttt tctctgcgtc gccccgacgg caatgaagtc cgcgcggaga cccttgaagg
     1441 ttggacctgc atgaaacgga ccgacctcct tgaattcatg gacagcctcg ttcaaccttc
     1501 cgccgtctgc cgcggcagga acttttcgca tttcctccgg gagaacggca gggtagtggc
     1561 cgcggtcttc tccaatggca cgatcgagta cggcgacatt ttcatcggct gcgacggcgg
     1621 caaatccaag gtgcgcgatg cggtcttcgg cagtcttccg gcaaccacca tcatggtgaa
     1681 ggagattgtg ggcatcgcga aaaacagcat gctgtggccg cgccaccgaa atagcttcgt
     1741 caagttccag catgaccagt acggcctgtc cttcggaatg atccctaccg gccccgatga
     1801 atttgtctgg ttcatgcaat ttgatccctg ccggggcgac atctccggga tagatcacga
     1861 gcagatccgg aaattcgtca tccgaagcct gagcggtttt cccagcgaag tatcggagtt
     1921 gctgcgatcc aacgatttca gccggaccta tgtgtggaat acccgcgact tcgaccttct
     1981 gcccgcattc caccgggata atattgtcct ggttggggac gcggcgcacc aggccttgcc
     2041 cttttccagc agcggcacca ccaacgccat cgtcggcgca gccgcgctct cgcatgcgct
     2101 cgacaactcc ctcaacctgg aagctgcctt tcaacaatat tattcccagc gcgctccggt
     2161 aatccgcgaa cacgtgcgca tcggccgtga actgagg
//
LOCUS       DATGKE010000211         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_129224, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000211 DATGKE010000000
VERSION     DATGKE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129224"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VKU83_03685"
     CDS             <1..196
                     /locus_tag="VKU83_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92677.1"
                     /translation="DGVLSNQTNMKPGFIITKIGDTRVRSVAELKDALGKQGSNFQIQ
                     GVYPDNSTEVYYYGINDFRK"
     gene            356..709
                     /locus_tag="VKU83_03690"
     CDS             356..709
                     /locus_tag="VKU83_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLI92678.1"
                     /translation="MSARTATTANESIVLDLVYAINAENFFEAARLLTDDFSFVGVLG
                     TRDGATAYVDDLKRMKIKYKIHKVLASDNDVCVLCDYTLQGKNLLGCNWYTIEQGKVS
                     SLKAVFDPRPLLEKP"
     gene            complement(725..2836)
                     /locus_tag="VKU83_03695"
     CDS             complement(725..2836)
                     /locus_tag="VKU83_03695"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLI92679.1"
                     /translation="MRKLLPLLLLPFYSLAQTGPFLPFKKYPFTTELTASATGERIAW
                     IMNEEGRRNIYVGEGPAFTPRKLTDFTEDDGQEISSLSISDDGRWVVFVRGGDHGANW
                     DDGLPVNPAFATEPFKVQVGCVAFSGGAVRWISEGDEPVVSPDGRRVAFIKGGQAWGA
                     VLDTIGPVKNLFTTRGSVGSLQWSPDGKRLLFVAGRGDHSLIGIYTVGSSVLKWIAPS
                     FHRDQSPRWSGDGSRIVFIRRPGAGGAPDSLLVARPNPWSIYVADTAGAAAVLLWKSA
                     VTLRGSYPSTDGGANLHWAAGNTIVFLSYQDGWPHLYSMDLAGKAVLLTPGNFMCEHI
                     RLSADKKHMLFSANTGPDPLDIERRHVGMVSVDHPDMQVMTPGSGLEWAPVTTGDGKA
                     LAFVSATPQRPPLPAILRFGGNGTEVLAADHIPADFPLNKLVIPRQVVFKSADGFLVH
                     ADLFEPAGGAARKPAVVYVHGGPPRQMLLGWHYSDYYSNAYAANQYLASLGFVVLSVN
                     YRLGIGYGYDFHQPPHAGASGAAEYKDIKAAGEWLHRQAFVDTAKIGIYGGSYGGYLT
                     ALALARDSKLFKAGVDFHGVHDWMGQTTLYNSFRDRYEKAPDYDLALKTAWLSSPVSA
                     IDTWRSPVLIIQGDDDRNVRFSQSVDLVQRLDRKGVPYETMVMVDDTHHWMKFANAVA
                     VYGAAADFFVRHFMAPVATRP"
     gene            complement(2860..>3083)
                     /locus_tag="VKU83_03700"
     CDS             complement(2860..>3083)
                     /locus_tag="VKU83_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92680.1"
                     /translation="LAERCGLELVYFERCKKALTLDYLHTQFRVYRHWLLTPVVGFLH
                     RLLPARVTGRNFYIRIGVMVVILELRKKP"
BASE COUNT          754 a          991 c          755 g          583 t
ORIGIN      
        1 cgacggtgtc ctgagcaatc agaccaatat gaagcctggc ttcatcatca cgaagatcgg
       61 cgacacgcgc gtaagaagcg tagccgagct caaggatgcc cttggcaaac agggaagcaa
      121 cttccagatc cagggggttt atccggacaa tagcacggaa gtctattact acggtatcaa
      181 cgacttccgt aagtaacaaa gatttaacag tcgaagctgc ctccggcggc ttgtccggaa
      241 ggccagacat aaattgttct ctcaatcgat aacactgacg cggccgctcc attcctgggg
      301 cggccttttt ttatcgacag gcatgaaaat tgccgccctt ctataaacga atgccatgtc
      361 cgcaagaacc gctactaccg cgaacgaaag catagtcctc gatctcgtct atgccataaa
      421 cgcagagaat ttcttcgaag ccgcccgcct gctgacggac gacttcagct tcgtcggtgt
      481 cctcggtacc cgcgacggcg ccacagctta cgttgacgac cttaaacgca tgaagatcaa
      541 atacaagatc cataaagtgc tggccagcga caacgatgtc tgcgtacttt gcgactatac
      601 cctgcaaggc aagaacctgc tgggctgcaa ctggtatacg atagagcagg gcaaggtcag
      661 ttccctgaag gcggtcttcg acccgcgccc gctcctggag aagccataac gctgatgatt
      721 gtcctcacgg acgagtcgcc acgggcgcca taaagtgccg cacaaaaaaa tccgcggccg
      781 cgccatagac ggccaccgca ttggcaaact tcatccaatg atgcgtatca tccaccatga
      841 ccatcgtctc ataaggaaca cccttacgat ccagccgctg cacgagatcg acactctggc
      901 tgaaacgcac attgcgatcg tcatcaccct gaataatgag caccggcgat ctccaggtat
      961 cgatggccga gaccggcgat gaaagccaag ccgtcttcag cgccaggtca taatccggcg
     1021 ccttctcata tctatccctg aaagaattgt acaacgtcgt ctggcccatc cagtcatgaa
     1081 cgccatgaaa atccaccccc gccttgaata gtttggaatc ccgcgccaac gccagcgccg
     1141 tcagatagcc gccataggag ccgccataga taccgatctt ggccgtatcc acaaaagcct
     1201 gccggtgcag ccattctccc gccgccttga tgtctttata ttcggccgca cccgatgcgc
     1261 cggcatgcgg cggctgatgg aaatcatagc catacccgat ccccaaccgg taattgacgc
     1321 taagcactac aaagcccagc gatgccaggt attgattggc ggcataggca ttggaataat
     1381 agtctgaata atgccaaccc agcaacatct gccgcggcgg tcctccatga acatacacaa
     1441 cggccggttt ccgggccgct ccgcccgccg gctcgaagag atcggcatgc accaggaacc
     1501 cgtcggcgga tttaaagacc acctgccgcg gaatgaccag cttgttcagc ggaaaatccg
     1561 ccgggatatg atccgccgcc aatacctcgg tgccatttcc cccaaatctc aaaatagcgg
     1621 gtaatggcgg ccgctgcggt gtggcgctta caaaagcaag cgcctttccg tcaccggtag
     1681 tgacaggcgc ccactccagc ccgctgccgg gtgtcatcac ctgcatatcg ggatgatcca
     1741 cgctcaccat tccgacatgc ctccgctcta tatcgagcgg gtccggtccg gtgttggcgc
     1801 taaaaagcat atgtttctta tcggcgctga gccggatatg ctcacacata aaattaccgg
     1861 gcgtcagcaa taccgccttc ccggccaggt ccatcgaata cagatgcggc cacccgtcct
     1921 gataagacaa gaacacgatc gtattccccg ccgcccagtg aagattcgcg cccccgtccg
     1981 tcgatggata ggacccgcgc agcgtaaccg ccgatttcca caacaacact gccgccgcgc
     2041 cggccgtatc ggctacataa atgctccagg gattgggcct ggctaccagc agcgagtccg
     2101 gcgcaccccc tgcccccggc ctgcggatga agacgatgcg gctcccatcc cccgaccacc
     2161 gcggcgactg atcccggtga aaagaaggtg cgatccattt cagcaccgat gatcccaccg
     2221 tatagatgcc gatcaacgag tggtcacccc ggcccgctac gaacagcagc cgtttcccat
     2281 ccggcgacca ttgcagcgac cccacactcc ctctcgtggt aaataagttc ttcaccggcc
     2341 cgatagtatc caaaaccgcc ccccaagcct gtccgccctt gataaaagcc acccgccggc
     2401 catccggcga gacgaccggc tcgtcaccct ccgagatcca acgcactgcc ccacccgaaa
     2461 aagcgacgca gcccacctgt accttgaacg gctccgtggc aaacgcagga tttacaggaa
     2521 gcccgtcatc ccaattggcg ccatgatccc cgccacggac gaacaccacc caacggccat
     2581 catcgcttat ggatagactg cttatctcct gtccgtcatc ttctgtgaaa tcggtgagct
     2641 tgcgtggcgt aaaggcagga ccttcgccca catagatatt ccggcggccc tcttcattca
     2701 tgatccaggc gatccgttca ccggtagccg atgccgtgag ctcggtagta aaaggatatt
     2761 tcttaaaggg tagaaaaggg cccgtctgcg ccagcgaata aaaaggaagc aacaggagcg
     2821 gcagtaattt cctcatgtac gtagtgtttg ccggataaat tacggctttt tgcgcaattc
     2881 caggataacc accatcaccc cgatccggat ataaaaattt cgccccgtca cccgcgcagg
     2941 cagcagccgg tgcagaaagc cgaccaccgg tgtcaatagc caatgccggt aaacccggaa
     3001 ctgcgtatgc agatagtcga gcgtaagcgc ctttttgcac cgttcaaaat agacgagctc
     3061 caaaccgcaa cgctccgcca gca
//
LOCUS       DATGKE010000212         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_470724, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000212 DATGKE010000000
VERSION     DATGKE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470724"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /locus_tag="VKU83_03705"
     CDS             <1..2086
                     /locus_tag="VKU83_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLI92681.1"
                     /translation="TYITVGLKKRPPTDLPEVTVLSRSADANVSEAKMSTVNIDPAQI
                     RKTPILFGEADLLKVLQLQTGITTIGEGAGGFSVRGGNADQNLILLDGAPLFNTSHLL
                     GFFSSISPEAVEDFTLYKGAIPAAFGGRLSSFVAINVRPGNPDTFRYGFSINPVSLHL
                     FAEGPVKKRKLTFSADGRVAFPKYIINQLPGSTSNSNAFFYDLTGKMVYHFNEKNQVA
                     VTLYRSYDLYKFPGDTSYTWQSDVVAVNGRDQLSHSLGLYYNGNLSYNSSNINGLQPS
                     YQFTIRSFIENREGKISLRYQPGEKLYAEWGGDFIHYSVGPGTLKPTNARSQINGQDL
                     RKEYGNELAGYGLVRYELTPLITLEAGLRYSAYQYLGPNTVYRYAPGVPPSPQTIIDS
                     VSFPKHKAIASYNGWEPRILLKFGIDDRTSIKLSYIRTQQFLQLVTNTTAITPVDYWK
                     LSDPMLQPAKADQWAAGFFKNFADNSFESSLEGFYKKSANLVDYANGASLSMNPQIDA
                     DLLSAKGKSYGIELNVNKTKGKTTWQLAYTWSRSLIADVTPYASQTVNGGSYYPSNYD
                     RPVNLSVTGGYKLGDGWELGMTFVYLSGRPTTYPDGDYLINGTVVTNYSTRNADRLPD
                     YNRLDVSFTYDSRRYSGQKKYYIFNFSIYNLYARQNAYSIYFQRNGGVLDAYQLSVFG
                     TIIPSCTLTYYF"
BASE COUNT          513 a          645 c          558 g          472 t
ORIGIN      
        1 cacctatatc acggtcgggc tgaagaagcg gccccccacc gacctgccgg aggtcacggt
       61 gctgagccga agcgcggatg ccaatgtctc ggaagcaaaa atgagcaccg taaacatcga
      121 cccggcgcag atccgcaaga cccccatcct ttttggcgaa gccgacctgc tcaaagtcct
      181 gcagctccaa accggcatta ccaccatcgg cgaaggcgcg ggtgggttca gcgtccgcgg
      241 cggaaatgcg gatcaaaacc tgattctgct cgatggagcc cccctgttta acacctcaca
      301 cctcctggga tttttttcgt ccatcagccc cgaagccgtg gaggacttca cgctctataa
      361 gggagccatc ccggccgcct tcggcggccg gctgtcctcg ttcgtagcca tcaatgtccg
      421 gcccggcaat ccggatacgt tccggtatgg gttcagcatc aaccccgtca gcctgcacct
      481 gttcgcggaa gggcccgtga aaaagcgaaa gctcacgttt tcagccgacg ggagggtggc
      541 gttccccaag tacatcatca accagcttcc cggcagcacc tccaacagca atgctttttt
      601 ctatgacctg acaggaaaaa tggtctatca cttcaatgag aagaaccagg tcgccgttac
      661 gctgtatcga agctatgacc tgtataagtt cccgggtgac accagttaca cctggcaatc
      721 ggacgtggtg gctgtcaacg gccgggacca gctcagccat tcactcggcc tctattacaa
      781 cgggaatctc agttacaatt cgtcgaacat caacgggctt cagccgtcct accagtttac
      841 gatcagaagt tttatagaaa accgggaagg caaaataagc ctccgttatc agccggggga
      901 aaaactatat gcggagtggg gcggagactt cattcattat tcggttggtc ccggcacgct
      961 gaaaccgacc aatgccaggt cgcagatcaa cgggcaggac ctgaggaagg agtacgggaa
     1021 cgaactggcg ggctacggtc tcgtccggta tgagctgacc ccgctgatca cgctggaggc
     1081 gggcctgcgc tattccgcgt atcaatacct gggaccgaac acggtgtatc gttacgctcc
     1141 gggggtcccc ccatcaccgc agaccattat tgattccgtc agttttccaa aacataaagc
     1201 gattgcatcc tacaatggct gggagccaag gatcctgctg aaattcggta tcgatgaccg
     1261 cacctcgatc aagttgagct atatccggac gcagcagttt ctgcaactgg tgaccaacac
     1321 gaccgccatc acgccggtcg attactggaa actgagcgac cccatgctgc aaccggcgaa
     1381 agcggaccag tgggcggcgg ggtttttcaa aaattttgcc gacaatagtt tcgagtcttc
     1441 cctcgaaggc ttttataaaa agtccgcaaa cctggtcgac tatgcgaacg gcgcttccct
     1501 gtcgatgaat cctcaaatcg atgccgacct gctttcggca aaaggcaaat cctatgggat
     1561 cgaactcaat gtcaataaaa ccaagggcaa gaccacctgg cagctggcct acacctggtc
     1621 gagatccctg atcgcggatg ttacccccta tgcctcccaa accgtgaacg gcggttctta
     1681 ttacccttcg aactatgacc ggccggtcaa cctcagcgtc accggcgggt acaagctcgg
     1741 cgacggctgg gagctgggca tgaccttcgt ctatctctcg ggcagaccga cgacctatcc
     1801 cgacggggat tatctcatca acggcacggt ggtgaccaat tattctaccc gcaacgcgga
     1861 cagactgccg gattacaacc gcctcgatgt ctccttcacc tacgacagcc gccggtattc
     1921 cggccagaag aaatactata tttttaattt ttcgatctat aacctttatg cgcggcaaaa
     1981 tgcctattcc atttatttcc agcggaacgg cggtgtcctc gacgcctacc agctttccgt
     2041 gttcggtacc attattcctt cctgcacctt aacctattat ttctaaatga agcggacgat
     2101 ggtcatatcg gtagtcggta ttctgctcca ggctgcttgt gtaaggcagg tgaaggttcc
     2161 catccgaaaa gtgaacggga tcctggtc
//
LOCUS       DATGKE010000213         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_336784, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000213 DATGKE010000000
VERSION     DATGKE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336784"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1076
                     /locus_tag="VKU83_03710"
     CDS             39..1076
                     /locus_tag="VKU83_03710"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HLI92682.1"
                     /translation="MNRKNFLTSLAGFAVMRGPGALPNARRASPQPLLIPPYLKAGDR
                     IGITCPAGDITLKDIEPAVQMLESWGFRTVAGNTIGKKDFIFGGTDAERTADFQEMLD
                     NPDIHAILCARGGYGSVHIVDQLNFSPLLQNPKWIIGFSDITVLLSHIHTNLHIAGIH
                     SKMCNSFPDDMTTAEPGQAASILSIRQAMAGEKMSYTVAPQPMNRPGTATGQLVGGNL
                     KTIESIAGTVSDIDTAGKILFLEDTDEYLYSIDRMFWHLQRTGKLDRIAGLIVGGFNV
                     RPDDPGEEFGRSIYDIVTDRVKLFNYPVCFDFPVGHQKENYALKHGVPHTLTVTTALV
                     QLSETANTVLP"
     gene            1073..1981
                     /locus_tag="VKU83_03715"
     CDS             1073..1981
                     /locus_tag="VKU83_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HLI92683.1"
                     /translation="MIVPPYLRPGDTIAIVCPAGYMAIEKAQTCIETLTKWGYRVRPG
                     ATLGGDSPNYFSGSDEARLADLQQMLDDPGVNAILCGRGGYGLSRIIDALDFTRFCEH
                     PKWIIGFSDITILHAHLQARYDVVSLHAPMAAAFNDGGASGPYVRSLRQALEGEDLRY
                     TCEPHRLNRAGQSAAPITGGNLALLAHLAGTASAAPTTGKILFIEDVGEYLYNIDRMC
                     RQLKRGGQLDGLAGLIVGSFTEGKDTVRPFGATADEIVREIIAEYDYPVCFGFPVGHG
                     GQNVALRTGAPHLLRVTGESVILQTA"
BASE COUNT          439 a          667 c          597 g          423 t
ORIGIN      
        1 taaatttttt ccagggcaaa gtaaacacta atgttaagat gaatcgaaaa aactttctca
       61 cctctctcgc tggatttgct gtcatgcggg gccctggcgc tttgcccaat gcacgccggg
      121 catcgccgca accgttgctc attccgccct atttgaaagc cggcgaccgc atcggcatca
      181 cgtgccccgc cggcgatatt acgctcaaag atatcgaacc cgccgttcaa atgctcgaaa
      241 gctggggatt ccggacggtg gccggcaata cgatcggaaa aaaggatttt attttcggcg
      301 gaaccgatgc cgagcggacc gcggacttcc aggagatgct ggacaacccg gacatccacg
      361 ccatcctgtg tgcccgtggc ggttacggtt ccgtccatat cgtcgaccag ttgaatttct
      421 ctccgctgct gcaaaatccg aaatggatca tcggcttcag cgatatcacc gtgctgcttt
      481 cccatatcca tacgaacctt cacatcgcgg gcatccattc gaagatgtgc aacagttttc
      541 ctgacgacat gacaacggcc gagccggggc aggcagcctc gatcctttcg atccgccagg
      601 cgatggccgg tgaaaagatg agctacaccg ttgcgccgca accgatgaac cgccccggga
      661 cggctacggg tcagctcgtc ggcggcaacc tgaaaacgat cgagagcatc gcgggcaccg
      721 tgtcggatat cgataccgcc gggaaaatac tttttctcga agacacggat gaatacctgt
      781 acagcatcga ccggatgttc tggcacctgc agcggaccgg aaaactcgac cggatcgccg
      841 gcctcatcgt cggcggattc aacgttcgcc ccgacgaccc tggcgaggaa tttggccgca
      901 gcatctatga tattgtcacg gaccgggtca agctcttcaa ctatccggtc tgctttgact
      961 tcccggtcgg ccaccaaaaa gaaaattatg ccctgaagca cggggtcccg cacaccctca
     1021 cggtgacgac cgccctggtg cagctgagcg aaaccgctaa taccgtcctg ccttgatagt
     1081 tccgccatac cttcgccccg gcgataccat tgccattgtc tgccccgccg gctacatggc
     1141 catcgagaaa gcgcaaacct gtatcgagac gctcacgaaa tggggatacc gtgtccggcc
     1201 gggcgcaacc ctcggcggcg actcgccgaa ttatttttcc ggcagcgatg aggcgcggct
     1261 tgcggacctg caacagatgc tggatgaccc cggcgtgaac gcgatccttt gcggccgcgg
     1321 cggctatggc ctcagccgca tcatcgatgc ccttgacttc acccggtttt gcgaacaccc
     1381 gaaatggatc atcggcttca gtgacatcac gatccttcac gctcatctgc aggcccggta
     1441 tgacgtcgtt tccctgcacg cgcccatggc ggccgccttc aatgacggcg gggcctcagg
     1501 cccatacgtc aggtcgctcc gccaggccct ggaaggcgag gacctgcggt acacctgcga
     1561 gccgcaccgc ctgaaccgcg ccgggcagtc ggccgccccg atcacgggcg gtaacctcgc
     1621 gcttctggcg cacctggctg gaaccgcttc ggccgcgccg acgaccggga aaatcctatt
     1681 catcgaagac gtaggggaat atttgtataa tatcgaccgc atgtgccggc aactgaagcg
     1741 aggcggccag ctggacggtc tcgccgggct gatcgtcggc agctttaccg agggaaagga
     1801 tacggtccgg cccttcggcg ccacggcgga tgagatcgtc agggaaataa tcgcggaata
     1861 cgactacccc gtttgctttg gatttccggt gggccacggc ggccagaacg tcgccttaag
     1921 gacgggtgcg ccgcatcttc ttcgcgtaac cggcgaatcc gttatcctgc aaacggcata
     1981 gttgcagccg cctgacgggt aagcttcgcg gctcaaccgc gggatggcct attgtcccgg
     2041 cggcggcacc acttcataag cgtcctggcc gtcctgctcg atcggttcgt agaaggtatt
     2101 gttgtaaaca aagatgtcct gtcctt
//
LOCUS       DATGKE010000214         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1233476, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000214 DATGKE010000000
VERSION     DATGKE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233476"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VKU83_03720"
     CDS             complement(<1..2154)
                     /locus_tag="VKU83_03720"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HLI92684.1"
                     /translation="MPRQSDDNKGFVHVRGAREHNLKNVSLKIPRDALVVFTGVSGSG
                     KSSLAFGTLYAEAQRRYLESVSPYARRLFHQMPVPQVEAVEGLPPAVVLQQQKGGVSA
                     RSSVGSVTTISNLMRMLYSRAGDYSAGHEIIYAEGFSPNTPEGACPACHGLGKVYEVT
                     EQSMVPDDSLTIRERAIAAWPLAWQGQNLRDILTTLGFDVDTPWRDLPKKDRDWILFT
                     EETPTVPVYAGFTAKEVKQAIRRKMEPSYMGTFTGARRYVLHTFANTQSALMKKRVSQ
                     YMLSTACPTCQGKRLRPESLAVKFAGLDIADMSRVPLKKLVSLFQPFVSPTGVEGTEK
                     AIVAARIAGDIVARLSVLLDLGLGYLTLERSTPTLSPGELQRLRLATQVYSNLFGVVY
                     VLDEPSAGLHPSDTEALLRALDKLKTSGNSLFVVEHELDIIRHADWIVDVGPDAGENG
                     GEILYSGPLEKLRSVGKSLTKDYLFATSGSGKNEYRSPRGWLKLNEVRINNLDGLNAA
                     FPLGVLTSVTGVSGSGKSSLVSHALVSLVADRIGAALAVREEASPMDAPQEEPTAMAE
                     GKIVAGLDSIRRLVVVDQKPIGRTPRSNLATYTGLFDGVRKLFAATKMAKSRHYDAGR
                     FSFNVGKGRCENCQGEGFVMVELLFLPSVYAPCPVCGGKRYNAPTLEVTYKDKNIAEV
                     LRMNVDEASNFFSADPLISRGLETLREVGLGYLRLG"
     gene            complement(2757..>2995)
                     /locus_tag="VKU83_03725"
     CDS             complement(2757..>2995)
                     /locus_tag="VKU83_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HLI92685.1"
                     /translation="EILADELEARSIKKTQFAAQLGMKLSHFSELLHGKRHVGAATAL
                     KLEKLLDIPAEYWMRVQVYHDLFVERHKKNEAAA"
BASE COUNT          693 a          875 c          712 g          715 t
ORIGIN      
        1 ccccaggcgc aaataaccca ggcctacctc ccgcagcgtc tccaatcccc ggctgatcaa
       61 tggatcggcc gaaaaaaagt tgcttgcttc gtccacattc attcgaagca cttcggctat
      121 gtttttgtct ttgtacgtca cttccaaagt tggagcattg tagcgcttcc ctccgcagac
      181 cggacatggc gcataaacgc ttggcagaaa caacagctcc accatgacaa atccctcgcc
      241 ctggcaattc tcgcaccggc cctttccaac gttgaacgag aaacgtcctg catcataatg
      301 ccgggacttt gccattttcg tagcggcaaa cagtttgcgc accccatcga ataacccggt
      361 ataggtcgcg aggttcgaac ggggtgtcct gccaataggt ttttgatcca ccaccaccag
      421 ccgcctgatc gaatccagac ccgcaacgat ctttccttcg gccatggccg taggctcttc
      481 ctgcggggcg tccatgggag aagcctcctc ccgcactgcc agcgcagctc cgatccgatc
      541 tgctaccagg cttaccaacg catgacttac aaggctggat ttacctgaac ctgagacacc
      601 cgtcaccgac gtcaataccc ccaaaggaaa tgcggcattt aaaccatcga ggttattgat
      661 ccgaacctca tttaatttca accagccacg aggagaccgg tactcgttct tacccgaacc
      721 cgatgtcgca aacaaatagt ctttggtcag ggatttcccg acgctacgta acttttccaa
      781 cggaccgcta tacaatattt caccgccatt ctcgcccgca tcgggcccta catccacgat
      841 ccaatctgcg tgacggatga tgtccagctc atgctctaca acaaaaagcg aattcccgct
      901 ggtcttcaac ttgtccaacg cccgtaacaa ggcctccgta tctgaagggt ggagcccagc
      961 cgagggttca tcaagaacat agacaacacc aaatagattg ctatatacct gcgtagccaa
     1021 cctaagcctt tgcagctctc cgggagataa agtaggcgtg ctccgttcca gtgtcagata
     1081 accaagacca agatcgagca gcacagacag tctggcaact atgtccccgg caatccgcgc
     1141 tgctacgata gctttttcgg tcccctcaac tccggtggga ctgacaaaag gctgaaatag
     1201 ggaaacaagc tttttgagtg gcaccctcga catatccgcg atatccagcc cggcaaactt
     1261 caccgccaac gactcgggcc ttagccgctt accctggcag gtagggcagg ccgtgctgag
     1321 catatattgg gacacccgct tcttcatgag cgcgctttgg gtgttggcaa acgtatgcaa
     1381 tacataccgc cgggcaccgg taaatgtgcc catataactg ggctccatct tacgcctgat
     1441 cgcctgcttc acttctttcg cagtaaatcc cgcatacaca ggtaccgtgg gcgtctcctc
     1501 cgtaaacaag atccagtccc ggtctttttt tggcagatca cgccagggcg tgtctacgtc
     1561 gaaccccaac gtggtaagga tatcccgcag attctgtccc tgccaggcca atggccacgc
     1621 cgcaatcgcc ctttcccgga tagtcaggga atcgtccggc accattgatt gctccgtcac
     1681 ttcatagact ttgccaagcc cgtggcaggc gggacaagcg ccttccggcg tattcggaga
     1741 gaacccttcc gcatagataa tctcgtgtcc ggcagaataa tccccggccc gggaatacaa
     1801 catccgcatc aagttcgaga tcgtcgtcac cgatccaacc gatgaccggg ctgacacccc
     1861 tcccttctgc tgttgcagga ctaccgcagg cggcaggccc tcaactgcct cgacctgcgg
     1921 cacgggcatt tggtgaaaga ggcgccgcgc gtagggcgaa acggattcca gatatcgacg
     1981 ctgtgcctct gcatacagtg ttccaaatgc cagcgacgat ttccctgaac cggatacgcc
     2041 ggtgaaaacc accaaagcat cccttggaat cttcaggctg acgttcttga gattgtgttc
     2101 cctggctccc cggacatgga caaatccttt gttatcatct gattgtcgtg gcatagctca
     2161 ttgattgcaa ttttgatacc gtctattttc cggcggctgg cgtggtcagt atcgccgcct
     2221 tgcccgtgag cgcgtataat tgcaatgatt ctattatcga gcagggtgaa atccaggtct
     2281 tctcaaaatc gataatgcgg gaagtagacc ttctttagaa ctgtacctcg tttgaacgcc
     2341 aggctatcgg aaagaatggg attcttggag atgcccagca acagccagct tggcacataa
     2401 tccttttcta tttgagtatc cttgagcttg tatagtttgt tataagatcc tcctctccaa
     2461 ggtgatcctt tatcggctca ggatcgatgc atatcggctc atattggctc atgagttcgt
     2521 tttcgtgcaa gcttattagg ttagggtata gtaagacccg ggaccggcgc catgacgccg
     2581 aatcatttta atttcgatca gtcggtcaag tggtcgtttc acggtggcaa tcgacactgc
     2641 cctgcggcgg gttgccctga agattgatac tcattggcag gctattttgt gtgagattaa
     2701 caaacctcac gcccgcgacg ctaccctggc ggatagaaag gaatgatatc gggaagtcat
     2761 gcggcggctt cgtttttctt atgcctttcg acaaaaaggt catggtagac ctgcacccgc
     2821 atccagtatt cagcgggaat gtccagcaat ttctccaact tcaaggcagt tgcagcacca
     2881 acatggcgct tgccatgcag tagctcgctg aaatggctga gtttcatgcc tagttgcgct
     2941 gcaaactggg tctttttgat cgacctggct tccagttcgt ctgctaaaat ttcac
//
LOCUS       DATGKE010000215         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_56200, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000215 DATGKE010000000
VERSION     DATGKE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56200"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1245)
                     /locus_tag="VKU83_03730"
     CDS             complement(94..1245)
                     /locus_tag="VKU83_03730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI92686.1"
                     /translation="MVLIFLSTLLGCCLLAGYGALIEYYRRAWQTLPEFDAAGRTGDI
                     RISVLVPARNEAANIAACIESLADQTYPKELFEVIVIDDHSTDGTAAIVRDLHPPGLR
                     LMLSRLAVSAAGPAPFKKLAIESGVRIATGDLIVTTDADCRFQPDWLNTLAVFYREKQ
                     AKFIAAPVRMSTAQHSLLAIFQMLDFITLQGITAASVAKKFHSMCNGANLAYDRAAFF
                     EVAGFRDIDDIPSGDDMLLMYKIYRKYPDAVYFLKSPAAIVSTKPEPGWTAFVNQRVR
                     WASKADRYDDRRIFRVLLLVYILNAALLLLPVAACWNRWWLWLFVAGLLVKTLLEYPF
                     VSSVAEFFGQESLMRWFPVLQPFHLVYTVVIGWMGKFASYSWKDRTVRR"
     gene            complement(1307..2125)
                     /locus_tag="VKU83_03735"
     CDS             complement(1307..2125)
                     /locus_tag="VKU83_03735"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLI92687.1"
                     /translation="MHAARFLPYLSSRVHYYTWGSGSRLLFAFHGYGESGSSFSFLGT
                     STPADLAVVAIDLPFHGRTEWNEGLKLEPQQLFQIMQSIADGLSGPGSQGPWSLLGYS
                     MGGRIALDLLHDYPDRFDRLILAAPDGMKVNPWYWIATATAFGNLLFRWSMQRPGLLF
                     FLLRCCHALHLVNPSVYKFAVHHIDDRKVRKELYLRWTAMRHFKPGLSRIAAIVRSRQ
                     LPVALIYGRFDRIIRWERGERFRKRAKESCRLILLDAGHQLLRQQYLPVFVALL"
     gene            complement(2195..>2442)
                     /locus_tag="VKU83_03740"
     CDS             complement(2195..>2442)
                     /locus_tag="VKU83_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92688.1"
                     /translation="GKLATPAGAQELRAEVFTLTACTGAVSVYNTAGAVMFRRSLPLN
                     PPSAIIQIPTAGWIAGEYILRVVTNLGNRATRAFMKF"
BASE COUNT          578 a          681 c          718 g          465 t
ORIGIN      
        1 caaaaaccgc cgtgaggatc gagcacccga tcactgccaa tccgaagagc gcgcctttat
       61 tgcgccagat gcgtttaccg acgtgctgtg ttctcatctt cttacagttc ggtctttcca
      121 ggaataactg gcaaattttc ccatccaccc aatgacgacg gtataaacaa gatgaaaagg
      181 ttgaaggacg ggaaaccacc tcatcaggct ctcctggcca aagaattccg cgacggaaga
      241 tacaaaagga tattccaaca gcgtcttcac cagcaacccg gcaacaaata gccatagcca
      301 ccaccggttc cagcaggcag ctaccggaag caggagcagc gcggcgttca ggatatagac
      361 cagcagcaat accctgaaga tccgccggtc gtcatagcgg tcggccttgc tggcccaacg
      421 gacccgctgg ttgacaaaag cagtccaccc cggctctggt tttgtcgaaa cgatggccgc
      481 cgggctcttg agaaagtaga cggcgtcggg atactttctg tagatcttgt acatcagcag
      541 catgtcgtct ccggagggaa tatcgtcaat gtccctgaag ccggcgactt cgaaaaacgc
      601 agcccgatcg taagcgagat tggcgccgtt gcacatggaa tggaatttct ttgcgaccga
      661 agcggcggtg atcccctgga gggtgatgaa atccagcatt tgaaaaatcg ccagcaggga
      721 atgttgcgcg gtcgacatgc ggacgggcgc cgcgataaat ttggcctgct tctcccggta
      781 aaaaaccgca agggtgttca gccaatccgg ctggaagcgg cagtccgcgt cggtcgttac
      841 gatcaggtcg ccggtagcga tcctcacgcc ggattctatt gccagtttct tgaaaggggc
      901 cggacccgca gccgaaacgg caagccgcga cagcatcagc ctcaatcccg gcggatgcag
      961 gtctctcacg atcgctgcgg ttccatcggt ggaatggtcg tcgatgacaa taacctcgaa
     1021 caactcctta ggataagttt ggtccgccag gctttcgatg caggcggcga tgttggccgc
     1081 ctcgttccgt gcaggaacga gcactgaaat gcggatatct cctgttctcc cggccgcatc
     1141 gaattccgga agggtttgcc atgcccttcg ataatattcg atcagggcgc catagccggc
     1201 cagcaggcag cagccgagga gcgtagagag gaagatcagg accatgagaa atgacgggta
     1261 aatatagggg aaagttgtca ggggcgttgg caagtcgacg gctgcgctac agcagcgcaa
     1321 caaaaacggg caggtattgc tgtcgcaata attgatgacc ggcgtccaaa aggatgaggc
     1381 ggcagctttc cttcgcgcgt ttgcggaacc gctcgccccg ttcccatcgg atgatgcggt
     1441 cgaatcgccc gtaaatgagg gcgacgggca gctgccggct gcgaacgatc gcggcgatcc
     1501 tggaaaggcc cggtttaaaa tggcgcatgg cagtccaccg caggtagagc tccttccgca
     1561 ctttgcggtc atcgatatga tggaccgcaa atttgtacac gctcggattg acgaggtgca
     1621 gcgcatggca gcaccgcaaa agaaaaaaga gcaagcccgg cctttgcatg gaccagcgga
     1681 aaagcaggtt gccgaacgcg gtggcggtag cgatccaata ccacggatta actttcatgc
     1741 cgtcgggagc ggcgaggata agccggtcaa agcggtcagg ataatcgtgc agaagatcga
     1801 gggcgatacg gccacccata ctgtagccca gcaggctcca cggcccctgc gacccggggc
     1861 cggaaagtcc gtcggcgatc gattgcatga tctgaaacag ttgctgcggc tccagcttga
     1921 ggccctcgtt ccattccgtc cggccatgga agggcaggtc gatggctacg accgccaggt
     1981 ccgcgggggt gctcgtccct aaaaagctga aagagctgcc tgattcaccg tagccatgaa
     2041 aggcgaagag aagcctcgac cccgatcccc acgtgtaata atggacgcgg gacgataaat
     2101 aaggaaggaa cctggccgca tgcatgacca agtataacga ttttttgcgt aacggttttc
     2161 cgccagtcgc ttttcggctg aacgcatggc ggcgtcaaaa tttcatgaat gccctggtag
     2221 cccggttacc caggtttgtc actacccgca ggatatattc gccggcgatc cagccggccg
     2281 ttgggatctg aatgattgcg gacggcgggt tgagcggcag cgatcgccgg aacatcaccg
     2341 cacctgccgt attgtaaacc gaaacggcgc cggtgcaggc tgtaagcgtg aagacttccg
     2401 cccgcaattc ctgcgcaccc gccggagtag ccagttttcc ga
//
LOCUS       DATGKE010000216         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_232378, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000216 DATGKE010000000
VERSION     DATGKE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232378"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..978)
                     /locus_tag="VKU83_03745"
     CDS             complement(229..978)
                     /locus_tag="VKU83_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92689.1"
                     /translation="MTKKTMGFSARRVFLSMMVCAGGLLATLSASAQLRDYLNLPDHD
                     SKRYYLGIGFLYEQSHLQVTPHPQFLKSDSVLSVTPLNTGGFGVSGMFTFRLADHLEF
                     RFAFPEFIFASNSLTYHVNYPPQGETNVASKQIQSLLLGFPAHVKFLSDRIGNFRVYM
                     LGGVNYQYDLASNSSTRKAQNLVKLTPADLSVEAGLGFQFYFPVFILSPELKFSEGIK
                     NVHSRDPNLQYSNVIDKLKTRMIVFSLIFEG"
     gene            complement(1077..1841)
                     /gene="ubiE"
                     /locus_tag="VKU83_03750"
     CDS             complement(1077..1841)
                     /gene="ubiE"
                     /locus_tag="VKU83_03750"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HLI92690.1"
                     /translation="MANYSHDRIVPFKDSELGKKQQIAEMFDKIALRYDFLNHFLSGG
                     IDLYWRRRAIRELRPAGGLSAGPLTVLDVATGTGDLAILLTRYLPGVQVTGIDISAGM
                     LGVGRQKVTRLQLDKQITLLTGDSEALQFPDGQFDAVTVAFGVRNFENLEKGLREMLR
                     VLKPGGKLVVLEFSKPRLPGIRQVYDGYLRLVAPKVGKMISSSREAYQYLNDSVRAFP
                     EGKAFTDILDSSGYSNPRLNRLSLGICSLYTGVKPA"
     gene            1871..>2456
                     /gene="yihA"
                     /locus_tag="VKU83_03755"
     CDS             1871..>2456
                     /gene="yihA"
                     /locus_tag="VKU83_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLI92691.1"
                     /translation="MDIHSSVYVVSSPDHTKCPKPDRPEYAFIGRSNVGKSSLINMLA
                     NNEKLAKTSGTPGKTQLINHFNIDDTWYIVDLPGYGFARASQTQRRKWEKMIEDYLRK
                     RENLVNVFALIDSRHSPQKIDLEFVNRLRTWGIPFTLVFTKADKENQRIVSANVKDFL
                     TKMRETWQFLPQHLVTSAVKRMGRDKLLDLIEGMN"
BASE COUNT          647 a          661 c          621 g          527 t
ORIGIN      
        1 tagcaccggc aaacctgcta acccaaaaag tacacctcca ccatgaatga gaccattgac
       61 gacctcctgt tgccgtgaat aaggatcggt ccgggaatac tgttccatat agtagggaat
      121 ttcaaccttt gtacaaacaa taaaatagaa aaatcatgcc accttgatag caataacaat
      181 caaggaattt ggtttgtttg tcgcagcgcc ggaagcgcag ggcgaatctt acccctcgaa
      241 gatcagcgag aagacgatca tccgcgtttt taacttatcg atcacatttg aatactgcag
      301 attgggatca cgactatgga cgttcttgat cccctcgctg aactttagct ccggcgacag
      361 gataaaaacg ggaaaataga actgaaaccc aagaccagcc tccacactca gatccgccgg
      421 ggtcagcttc accaggttct gcgccttccg ggtggaagag ttcgaggcca gatcgtactg
      481 atagttgaca ccgcccagca tatagacccg gaaattgccg atgcgatcgg acaggaattt
      541 tacatgcgcc ggaaaaccca gtaaaaggga ctggatctgc ttggaggcta cgttggtttc
      601 gccctgcggc gggtaattca catgatacgt taggctgttg ctggcaaaaa tgaactccgg
      661 aaaagcgaat cggaactcca ggtgatcggc tagccggaag gtgaacatgc ccgagacgcc
      721 gaaaccgccg gtattcagcg gcgtcacgga tagaacactg tcgcttttca ggaattgagg
      781 atgcggcgtt acctgcaaat ggctctgctc ataaagaaag ccgatgccca gataatagcg
      841 cttgctgtcg tgatcgggta agttgagata atccctcaat tgcgccgaag cggacagggt
      901 cgccagcaac ccgccggcac ataccatcat cgataggaaa acccttctgg cagaaaaccc
      961 catagtcttc ttagtcattt agaatcaatt tgttactgca actgggtagt aaatatctat
     1021 aggcttgact ttggggtggt aattataacg gctatgccgg caaataattg cctttgttaa
     1081 gctggtttaa caccggtata taacgagcag attcccaggc tcaaacgatt caatcgcggg
     1141 ttcgaataac cgctggaatc caggatatcc gtaaaagctt tcccttccgg gaacgccctg
     1201 acggagtcat tcagatattg atacgcttcg cggctactgc tgatcatttt acccactttg
     1261 ggggctacca gccgcagata gccgtcatag acctgacgaa tgccgggaag acgcggcttg
     1321 gagaactcca gcaccaccaa tttcccaccg ggtttcaata cccggagcat ttctctcagc
     1381 cccttttcca ggttctcgaa attgcggacc ccaaaggcaa cagtgacggc gtcgaattgt
     1441 ccatctggga attgcaacgc ttcactgtcc cccgtgagca gggtgatctg cttgtccagc
     1501 tgcagccggg tgaccttctg acggcccacc cccagcatac cggcagagat atcgataccg
     1561 gtgacctgca cccccggcag atagcgcgta agcaggatcg ccagatcgcc cgtaccggtg
     1621 gccacatcca atacggtcaa cggcccggct gacaggccac ccgctggacg cagttcccgg
     1681 atcgccctcc ggcgccagta cagatcgatc ccgccggaaa ggaaatgatt gagaaaatca
     1741 taccgcaatg cgatcttgtc gaacatctcc gcgatctgct gtttcttgcc cagctccgaa
     1801 tccttgaaag gcacgatcct gtcatgggaa tagttggcca taggagggca aaggtacgta
     1861 tcttgtggcc atggacatcc attcctccgt ttatgtggtg agcagcccgg atcacacgaa
     1921 atgtcccaaa cccgaccggc cggaatatgc ctttatcggg cgcagtaatg tcggtaagtc
     1981 ttcgctcatc aatatgctcg cgaacaacga aaaattggcg aagacctcgg gtacgccggg
     2041 caagacgcag ctcatcaacc acttcaatat tgatgatacg tggtatatcg tggacctgcc
     2101 cggctatggg ttcgcccggg cgtcccaaac ccagcgcagg aaatgggaga aaatgatcga
     2161 ggattatctg cgcaaacgcg agaacctggt caacgtcttc gcgctcatcg acagccggca
     2221 ttcaccgcaa aagatcgatc tggaattcgt taaccggctg agaacgtggg gcatcccctt
     2281 taccctggtc tttacaaagg ccgataagga aaatcaacgc atcgtgagcg ccaatgtcaa
     2341 ggattttctg accaaaatgc gggagacctg gcagttcctg ccgcaacatt tggtcaccag
     2401 tgccgtaaaa agaatgggga gagacaaact gctggacctc atcgaaggaa tgaacc
//
LOCUS       DATGKE010000217         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_788138, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000217 DATGKE010000000
VERSION     DATGKE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788138"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VKU83_03760"
     CDS             <1..872
                     /locus_tag="VKU83_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLI92692.1"
                     /translation="VRQSYYGGDSSQYQVPNMADFAFLSQYLNAGGAGENSGTGAGGA
                     KAGRAGAAGGLARIEHGFIDSNQQRLRVSINMADIGSKRLPVVLADLQKKTRELFDST
                     RYKVQFTGTSVTYLEGSAFVIKGLRDSIQWSFLLIAACMLLLFRSVRILFCSLAPNVI
                     PLIVTAGIMGWAGVRLKPSTVIAFSIALGIAIDITIRFLVNYKQERPGAGGASRAVVD
                     TINKTGISIIYTSLVLIAGFVIFCFSDFGGILALGWLTSITLVVATVTNLVFLPVLLL
                     TLTRSRHARSDVG"
     gene            complement(869..1528)
                     /locus_tag="VKU83_03765"
     CDS             complement(869..1528)
                     /locus_tag="VKU83_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92693.1"
                     /translation="MAVATKKIQVGIVDDHSLLRKALAKLISSFESYAVLFEGDNGKE
                     IKAKISQHLIPDIILLDVNMPDMDGYETVRWLHKNYPQIKVLALSMFSDENTIIRMLR
                     LGAKGYILKNIEPEELKLALDSIMKKDFYLSEYISGKIISGLHKDLGNPEDPIVLNDK
                     EKEFLRLVCSELSYKDIADKMFISPRTVDNYRNTLFEKLKVRTRVGLVMFAIKNGLVE
                     I"
     gene            complement(1550..2353)
                     /locus_tag="VKU83_03770"
     CDS             complement(1550..2353)
                     /locus_tag="VKU83_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLI92694.1"
                     /translation="MPAQNQEVYVYVVILISIALALLLVGFIVTTLFLYQRRQHRQEQ
                     QLARMKDEYQQELLRSQLEIQEATFKTIAMELHDNIGQVLSVVKLSLSVLPIDKDHPA
                     WESVQNSRQMLNKVIYDMGNLTKSLHTDRIAQIGLVEAMRFDLDSLRRTGLLEVEFSV
                     EGKEYRLEDQRSIFLYRMFQEMLNNILKHSKATRVNIAINYSIDNKFVLKVDDNGVGF
                     DIEKKRTQASSSSGIGLKSMTNRASLIGAQISVQSQPGKGTSIQVEVPL"
     gene            complement(2317..2976)
                     /locus_tag="VKU83_03775"
     CDS             complement(2317..2976)
                     /locus_tag="VKU83_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92695.1"
                     /translation="MPHYLFYFSPIAEAICFLFSLTIFFQPGAERYLKYFSGFLFVNL
                     LLDVSTAYTALNHINNVFVNNLVSLVVISFELYLIREIISGKNAKRIFLYIFLPYPVV
                     TILNIFFWQQVVNFHTMTYSLGALLIVSGCIYYFWELFRQRTSVDLVRQPAFWICSGL
                     LFYYSCTFGLYGLINFISNLPQNGIETLFIISIIINVCLYSSFTIAFICRLKTKRSMS
                     T"
     gene            complement(3095..>3315)
                     /locus_tag="VKU83_03780"
     CDS             complement(3095..>3315)
                     /locus_tag="VKU83_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92696.1"
                     /translation="ASEPEFEGRQTLVMVGNRSNDGTYSSSKELYSNKNGKPEIVAFA
                     GGVICPPFCGSLSKGSLVVRGDNRMEII"
BASE COUNT          804 a          825 c          890 g          796 t
ORIGIN      
        1 ttgtgcgcca gtcctattac ggcggcgaca gttcacaata ccaggtgccg aacatggccg
       61 attttgcatt cctttcccag tatctgaacg cgggcggcgc cggggaaaat tcaggaaccg
      121 gagccggggg tgcaaaagcc gggcgagccg gcgcagccgg cggcctcgcg aggatcgagc
      181 atggttttat cgacagcaat cagcagcggt tgcgggtcag catcaacatg gccgatatcg
      241 ggagcaagcg actgcccgtc gtcctggccg acctgcagaa aaagacgcgg gaactgtttg
      301 attccacgcg atacaaggtc cagttcaccg ggaccagcgt gacttacctg gaaggaagcg
      361 ctttcgtgat caaaggactg cgggacagca ttcaatggtc cttcctcctg atcgcggcgt
      421 gcatgctgct gctgttccgt tcggtccgga tcctgttttg ttccctcgcg cccaacgtca
      481 tccccctcat cgttaccgct ggcatcatgg ggtgggccgg cgtacggctc aagccttcga
      541 cggtgatcgc gttcagcatt gccttaggca tcgcgatcga cattacgatc cggttcctgg
      601 tcaactacaa gcaggagagg cccggcgccg gcggcgcctc gcgggcggtt gtcgacacga
      661 tcaataaaac ggggataagc atcatctata cgtccctggt gctgatcgca ggctttgtca
      721 ttttctgctt cagcgatttt ggcggtattc ttgccctggg ctggctgaca tcgatcacgc
      781 tggtagtcgc gaccgtgacg aacctggtct ttttaccggt tctgttgctc acgctgaccc
      841 gatccaggca tgcgcgttct gacgtcggct aaatctcaac aagcccgttc ttgatcgcaa
      901 acatcacgag gccaacccgc gtgcggacct tcaacttctc gaaaagcgtg ttgcgataat
      961 tgtcaactgt ccgcgggctg atgaacatct tgtcggcaat atccttgtac gaaagctcgg
     1021 agcaaaccag gcggaggaat tccttttcct tatcgttaag aacgatcggg tcttccgggt
     1081 tgccgaggtc cttatgaaga ccgctgatta ttttcccgga gatgtattca gaaagataga
     1141 aatccttttt catgatcgag tccagggcaa gcttcagctc ctccggctcg atattcttca
     1201 ggatataacc cttcgccccc agccttagca tccggatgat ggtattttcg tcgctgaaca
     1261 tggaaagggc gagtactttg atctggggat agttcttgtg cagccagcga accgtttcgt
     1321 agccgtccat gtcgggcata ttgacatcca gcaggatgat atcgggaatg aggtgttggg
     1381 agatcttggc cttaatttcc tttccgttat cgccctcgaa aagaacggca tagctctcga
     1441 acgagctgat cagtttggcg agtgccttgc gcaggagact gtgatcgtct acaattccga
     1501 cctgtatttt ctttgttgct acggccataa cgttcgataa tatttccaat tagagcggaa
     1561 cctctacctg gatagaagta cccttacccg gctggctttg aacactaatt tgagcaccga
     1621 tcagggaagc acggttcgtc atgctcttca ggccgatacc actggaggat gaagcctggg
     1681 tgcgcttctt ttcgatgtca aagccgaccc cgttgtcatc caccttcaga acaaatttat
     1741 tatcaattga gtaattaatc gcaatattta cgcgggtggc cttcgaatgt ttcaaaatat
     1801 tgttgagcat ctcctggaac attctataaa gaaagataga ccgttgatct tcaaggcggt
     1861 attcctttcc ttcaaccgag aattcgactt cgagaagacc cgtgcggcgc agactgtcga
     1921 gatcgaagcg catcgcttcg acgagcccga tctgggcaat gcggtcggta tggaggcttt
     1981 tggtcagatt acccatgtca tagataacct tattcagcat ctggcggctg ttctgcacgc
     2041 tttcccacgc cggatggtcc ttgtcaatgg gcagcacgga gagcgataac ttcactacgg
     2101 acagcacctg gccgatattg tcatggagtt ccatggcgat cgttttgaag gtcgcctcct
     2161 ggatctcgag ctgggatctg agcagttcct gctgatactc atctttcatc cgcgccaatt
     2221 gttgctcctg acggtgttga cggcgctggt aaaggaacag cgtcgtgacg ataaacccga
     2281 caaggagtag cgcaagcgca atagagataa gtatcactac gtagacatag acctcttggt
     2341 tttgagccgg catataaagg caatagtgaa cgacgaatat aaacagacat ttattatgat
     2401 cgatattata aacagggtct caatcccgtt ttggggcaga ttgctgatga aattaatgag
     2461 gccgtagagc ccaaatgtac aggagtagta gaacagcagt ccggagcaga tccaaaaggc
     2521 cggctggcgc acgaggtcga cagaagttct ttgccggaac agttcccaga aataatagat
     2581 gcagccgctt acaatcaaca atgcgccaag gctgtaggtc atcgtatgga aattgacgac
     2641 ttgctgccag aagaaaatat tgaggatagt cacgactgga taaggcagga aaatatagag
     2701 gaagatccgc ttggcattct tgccggaaat gatctcgcgg atcagataga gctcaaagga
     2761 gatgaccacc agtgacacca gattatttac aaagacgttg ttgatgtggt taagagcggt
     2821 gtaggcggtg ctcacgtcca gcagcaggtt gacaaaaaga aagccggaaa aatatttcag
     2881 gtagcgctcc gcgcccggct ggaagaatat ggtcaggctg aatagaaagc aaatggcttc
     2941 agcaattgga ctaaagtaaa acaggtaatg gggcataaga caaaggaacg ataattgccg
     3001 gtattttatg agtcaggacc tgccaaaaaa tgaaagccgt gctgcacagg gcaacacggc
     3061 ataatgatcc gatcaatggt tatggatact acgtttagat gatctccatg cggttatcgc
     3121 cgcgaactac aagcgatccc ttgctcagtg agccacagaa cggggggcag atgacgccgc
     3181 cggcaaaggc tacgatctcc ggtttaccgt tcttattgga atagagctcc ttgctgctcg
     3241 agtaggtgcc atcgttgctc cggttgccaa ccatcaccag ggtctggcgg ccttcaaatt
     3301 ccggttcgct ggcat
//
LOCUS       DATGKE010000218         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1168384, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000218 DATGKE010000000
VERSION     DATGKE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168384"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VKU83_03785"
     CDS             <1..641
                     /locus_tag="VKU83_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92697.1"
                     /translation="QQSRVAAVLLLTLRGTPTLYYGDELGMRDVPIPPEEVRDPQGLN
                     MPGKNLSRDPERTPMLWDAHPNAGFSATKPWLRVDPTWQRCNVSTQKNDDYSQLHLYK
                     KLIDLRQHSPALKTGTYRPVYSDQQLIAYIRYTEDQPAFLVVLNLTHRPGYFTPSTIS
                     FTGVIIIDTLPENEGSSVKNAIYLFGDEAMVIRLDEWQAISTSPPHEPSTMP"
     gene            619..>2011
                     /gene="treZ"
                     /locus_tag="VKU83_03790"
     CDS             619..>2011
                     /gene="treZ"
                     /locus_tag="VKU83_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HLI92698.1"
                     /translation="MNPPQCHNNGCLFRVWAPLQQSLSLQLQDKRIPMRPSPGGYFTA
                     DVPEIGDGARYHYIIGNDPYPDPASAYQPEGVFGPSQVVDHRRFPWTDAGWKGQPMED
                     LIFYELHIGAFTAEGAFDAIIPRLDDLARLGINAIELMPVAAFPGDRNWGYDGVFLYA
                     VQHSYGGPDGLKRLVDAAHRRGISVFLDVVYNHVGGEGNCLETYGPYFSESYRTPWGK
                     AINYDGAWSDGVRDFIIGNVLYWAEHYHLDGVRLDAIHEIYDRNAVTIWDELHAAVKR
                     WEKKNGRSCWLVAESDMNDPRVIQPPGKNGKGLDAQWMDDFHHALFTLLYPKEQKHYV
                     DFGTTEQLAKAMSAGFVHSGEYVKARIRRHGASSAAIGGEHFVVFVQNHDLPGNRPGG
                     ERLSVLIGDQALKLAAAAVLLSPYIPMLFMGEEYGEEAPFYFFCEYKDQDTINALREG
                     RKKQLAGYGYTEES"
BASE COUNT          437 a          602 c          542 g          430 t
ORIGIN      
        1 ctcagcaatc ccgggtcgcc gctgtgctgc tgctgacgct tcggggtacc cctaccctgt
       61 actatggaga tgagctgggc atgcgcgacg tgcccattcc accggaggag gtcagagacc
      121 cacagggact gaatatgccc ggtaagaacc tgagccgcga tcccgaacgg actcccatgc
      181 tctgggacgc ccatcccaac gccggttttt ccgctactaa accctggctg cgcgtagatc
      241 ctacctggca acgttgcaat gtgtccaccc aaaaaaacga tgactattcc cagctgcatt
      301 tgtataaaaa gctcatcgat ctccgccagc attcgcctgc attaaagaca gggacctacc
      361 gtcccgtata ttctgatcaa cagctgatcg cctacatccg ttataccgaa gaccagcccg
      421 cctttttggt agtgctcaat cttacccatc ggccaggcta tttcacgcca tcgaccatta
      481 gctttaccgg cgttatcatc atcgatacct tgcccgaaaa tgaaggctca tcggtgaaga
      541 acgcaattta tctctttggc gatgaagcca tggtcatccg gctggacgag tggcaggcga
      601 tctctaccag tcctccgcat gaaccctcca caatgccata acaacggatg cctgttccgc
      661 gtatgggcgc ctctacagca gagcctgagc ctgcagctgc aggacaaacg tattcctatg
      721 cggccgtctc ccggcggata ttttaccgcc gatgtaccgg aaataggcga tggcgcccgt
      781 taccattaca ttatcggcaa tgacccctat cccgatccgg cttcggctta tcagccggaa
      841 ggtgtattcg gcccctctca ggtagtcgac caccgccggt tcccctggac cgacgccggc
      901 tggaaaggac agccgatgga agacctgatc ttctatgagc tccacatagg cgcctttact
      961 gcagagggtg ccttcgatgc cattatcccg cgtttggatg acctcgcccg gctcggcatc
     1021 aacgccatcg agctgatgcc cgtggcggca tttccgggtg accggaactg gggctatgac
     1081 ggcgtctttc tctacgccgt gcagcacagc tacggcggcc ccgatggttt gaaacgcctc
     1141 gtcgacgcgg ctcaccgccg tggcatctcg gtctttctgg atgtcgtgta caatcatgta
     1201 ggcggcgaag gaaattgcct ggagacctac gggccgtatt tttcggaaag ctaccggacc
     1261 ccctggggaa aggccattaa ttacgacggc gcctggtcgg atggcgtacg cgacttcatc
     1321 atcggcaacg tactttattg ggcggaacac tatcatctcg acggggtccg gctggatgcc
     1381 atccatgaga tctacgatcg taacgcggtc acgatctggg atgaactgca tgcggccgtt
     1441 aaacgctggg agaaaaaaaa cggccggtcg tgctggctcg tggccgaaag cgatatgaac
     1501 gatccccggg tcatccagcc acccggcaaa aatgggaaag ggctggatgc ccaatggatg
     1561 gacgattttc accacgcgtt gttcacgctc ctctatccaa aagaacaaaa gcattatgtg
     1621 gacttcggaa caaccgaaca attggccaag gccatgtcgg ccggcttcgt tcattccggc
     1681 gaatatgtta aggctcgcat ccggcggcat ggcgcttcat cggcggccat cggtggtgaa
     1741 catttcgtcg tatttgttca aaaccacgac ctgccgggaa accgcccggg cggcgaacgg
     1801 ctgtccgttc tgatcggcga tcaggcgctc aaactagcgg cggcggccgt cctcttgtct
     1861 ccttacattc cgatgctgtt catgggcgag gaatacggcg aggaagctcc cttctatttt
     1921 ttctgcgagt ataaggacca ggacaccatc aacgcgctgc gcgaaggccg caagaagcag
     1981 ctcgcgggat atggctacac cgaagaatcg c
//
LOCUS       DATGKE010000219         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1154955, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000219 DATGKE010000000
VERSION     DATGKE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154955"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VKU83_03795"
     CDS             complement(<1..415)
                     /locus_tag="VKU83_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92699.1"
                     /translation="MQKTDNLGVIFITHDLGVVAEIADRALVMWKGRVVEQATVTELF
                     ARPQHPYTKGLLNCRPALHARGERLPMVSDFIGADAGQGNMPTAGRDRQTGNGKAGGP
                     TPAGGPTPCATGTMNAGAPGVMMRVEDLRVWYPSRR"
     gene            complement(428..1021)
                     /locus_tag="VKU83_03800"
     CDS             complement(428..1021)
                     /locus_tag="VKU83_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI92700.1"
                     /translation="MLSIQGLTIGFDTGNGLTAGVEDIGFEVNRGEMVALVGESGSGK
                     SITALSILNLLPVPPARVLAGRILFTGRDGRTIDLLRQPPAVMRKVRGAGIAMIFQEP
                     MTSLNPVFSCGDQVAEAIRTHRHQGAANARREAIALFEKVRLPDPERIFSSYPHQLSG
                     GQKQRVMIAMAISGGPSMLICDEPTTALDVTVQKTIL"
     gene            complement(1023..1793)
                     /locus_tag="VKU83_03805"
     CDS             complement(1023..1793)
                     /locus_tag="VKU83_03805"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HLI92701.1"
                     /translation="MKTKEEIVTNWLPRYTGEKLENFGKYILLTNFDNYVHLFADWHG
                     VQVVGEHRPMQCATADDISIINFGMGSPGAATVMDLLTAIQPKAALFLGKCGGLKKRN
                     KLGDLILPIAAIRGEGTSTDYFPPEVPALPAFALQKAISTTIRDYAVDYWTGTVYTTN
                     RRVWEHDDVFKEYLQKIRAYAIDMETATIFSVGFYNKIPTGALLLVSDQPMVPDGVKT
                     TESDIKVTGEFVERHIRIGIDSLKQLINNGLTVRHLRF"
     gene            1825..2268
                     /locus_tag="VKU83_03810"
     CDS             1825..2268
                     /locus_tag="VKU83_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HLI92702.1"
                     /translation="MIKIYYPTHSFKLREPEKKKEEIWDELRRQWVRLTPEEWVRQNF
                     IQYLLQVKNYPASYIAVERKMKLGELIKRFDLMVFDNAAQPWMLIECKAMGETLDHRV
                     LWQVLRYNMAIPVKYIVITNGEECHAFARGLTDFDGIPALPSYNE"
     gene            complement(2293..>2863)
                     /locus_tag="VKU83_03815"
     CDS             complement(2293..>2863)
                     /locus_tag="VKU83_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLI92703.1"
                     /translation="REIPCGQGQWFDQSNAWMSYGAQAARALKAQWHLTAVSGIGLIH
                     SCCQMSITMPEVYDRMDNRGNQGDWDFRQYQPDVVTVCLGQNDGIQDSAVFCSAYVRF
                     VGKIRRENPKAQIVLLTSPMGNAQLTEVLKRYLAGVVAAERKSGDKKVADYFFTKQWS
                     HGCAGHPDLADQGEIAAELTPYLRKLMGW"
BASE COUNT          570 a          915 c          749 g          629 t
ORIGIN      
        1 tccgccggga aggataccag acccgcagat cctccacccg catcatcaca ccgggtgcgc
       61 ccgcgttcat ggtccccgtg gcgcagggcg tcgggcctcc cgcgggtgtc gggcctcccg
      121 ccttgccgtt gccggtctgc cggtcccggc ccgcagtcgg catattgcct tgcccggcgt
      181 ccgcgccgat aaaatcactc accatcggca gcctttcccc tctcgcgtgc aaggccgggc
      241 ggcagttcaa caaacccttt gtatagggat gttgcggccg cgcgaacagt tccgtgaccg
      301 tcgcctgttc caccaccctg cctttccaca tcaccaatgc ccggtcggcg atctccgcca
      361 cgacccccag gtcatgcgtg ataaagatca cgcccaggtt atcggtcttt tgcaaatccc
      421 tgatcagcta caggattgtt ttttgtaccg tcacatccag cgccgtcgtc ggttcatcgc
      481 agatcagcat ggatggtccg ccgcttattg ccatcgcgat catcacccgc tgcttctggc
      541 cgccgctcag ctggtgcgga taactcgaaa agatgcgctc cggatccggc aatcttacct
      601 tttcaaaaag ggcaatcgct tcccgccggg cattggcggc tccctgatga cgatgggtcc
      661 ggatggcctc ggccacctgg tcgccgcagg aaaacaccgg gttgagggat gtcatcggct
      721 cctggaagat catcgcgatc ccggcgccgc ggaccttccg catcactgcc ggcggctgcc
      781 gcaacaggtc gatggtcctt ccatcccgcc cggtaaagag gatccggccg gcgaggaccc
      841 gcgccggcgg cacgggcaat aaattcagga tcgacaaggc cgtgatcgac ttccccgacc
      901 cggactcgcc aaccaacgca accatttctc cccggttcac ttcgaacccg atgtcttcga
      961 cccccgcagt cagcccattg ccggtgtcaa aaccaattgt taagccctgg atcgataaca
     1021 tgtcaaaatc gcaagtgcct taccgtgagc ccgttgttga tcagctgctt caatgagtcg
     1081 atgccaatcc ggatgtgccg ctccacaaat tcccccgtca ccttgatgtc gctctcggtg
     1141 gtcttcacgc cgtcaggcac catcggctga tcactcacga gcaacaaggc gcccgtggga
     1201 attttgttat aaaaaccaac cgagaagatc gtcgccgtct ccatgtcgat cgcgtaggcc
     1261 cggatcttct gcaggtattc tttaaagacg tcgtcatgct cccaaaccct cctgttggtc
     1321 gtgtaaacgg ttcccgtcca gtaatcgact gcgtagtccc ggatcgtcgt cgagatcgct
     1381 ttctgcaggg cgaaagccgg cagcgccggt acctccgggg ggaaataatc ggtcgatgtt
     1441 ccctcgcccc ggatcgctgc aatgggcagg atcaggtctc ccagtttatt gcgtttcttc
     1501 aacccgccgc atttgccaag gaagagggct gctttcggct ggatcgccgt gagcagatcc
     1561 atcaccgtcg ccgcacccgg actccccatt ccgaaattaa tgatggagat atcatcggcg
     1621 gtggcgcact gcatgggcct atgttcaccg acaacctgga ccccatgcca gtccgcaaac
     1681 aagtggacgt aattgtcaaa attggtcagc agaatatatt tcccaaaatt ttcgagcttc
     1741 tcgccggtgt acctcggcag ccagttggtg acgatttcct cttttgtctt cattattcgt
     1801 aaatttagca aatagtttct ggtcatgatc aaaatctact atcctacgca ttcctttaag
     1861 cttcgggaac cagagaaaaa gaaggaagag atctgggatg agctgcgccg tcagtgggtg
     1921 cgtctcactc cggaagaatg ggtccgccag aatttcatcc agtacctgct gcaggttaag
     1981 aactatcccg cctcctatat tgccgtggaa agaaagatga agctcggcga gctgatcaaa
     2041 cgcttcgacc tgatggtctt cgacaacgcc gctcagccct ggatgctcat tgaatgcaag
     2101 gccatggggg agacgctgga ccaccgggtc ctctggcagg tcctccgtta taacatggcc
     2161 atccccgtga aatacatcgt cattaccaat ggcgaggaat gtcatgcgtt tgcccgcggc
     2221 ctgaccgact tcgacggcat tccggcactc cccagctaca atgaataaat ttgccgtacc
     2281 ggtttcggcg ccttaccagc ccatcaattt cctcaaatac ggcgtcagct cggctgcgat
     2341 ctcgccctgg tccgccaggt ccggatgacc cgcgcagcca tgggaccact gtttcgtgaa
     2401 aaaataatcc gcgacttttt tgtcgcccga tttccgctcc gcagccacca cgccggccag
     2461 gtaccgttta aggacttccg ttagctgcgc attgcccatc gggctcgtca gcaggacgat
     2521 ctgggccttc gggttttccc ggcgaatttt gccgacgaac cggacgtaag cggaacagaa
     2581 taccgccgag tcctggatgc cgtcattttg ccccaggcag acggtcacga catccggctg
     2641 gtattgcctg aagtcccaat cgccctggtt gccgcggttg tccatccggt catagacttc
     2701 cggcatcgtg atgctcatct ggcagcaact gtggatcaat ccaattccgg acaccgcggt
     2761 cagatgccac tgcgctttca gggcccgggc ggcctgcgca ccgtaactca tccaggcatt
     2821 gctctgatcg aaccattgtc cctggccgca ggggatctcc cgg
//
LOCUS       DATGKE010000220         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_646527, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000220 DATGKE010000000
VERSION     DATGKE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646527"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VKU83_03820"
     CDS             complement(<1..1208)
                     /locus_tag="VKU83_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HLI92704.1"
                     /translation="MTNRLTFSALICLVCTSVTAQRHGSFRTPMDDSTLMEQSGLTMM
                     PYNRLIHSAGKVVSFGDPRQENHALDFSILPDGRHIAVEDRYGVAVLDMRSETIVHRW
                     TFREGTEQAVHGLMSTYCGIKSFGYDGQTYIVWGAGSTHSSALMIAEWSAAGFGKVSS
                     VPLNAAPPAAAALPNDIAVRTEDGQPYVYVVLNGNNQLLKIRFADRQIVWTAATGVAP
                     YGLRVIGGKAYVTNWAGPLVTDSTLEHAGTPWGSAYTSPVTGATARGSLSIIDAGTGR
                     LLNEVSLGLHPNAIAAGPDSAFLYICNGNSDYVSVFNVSREAVVDSIPVGLFSGSLTY
                     YGSSPDALAVDSSGTRLYVANGFDNAVCEIRLGAGMATGGNGRTAVKGYIPTEAYPGG
                     VCLAGGMLYVT"
     gene            1309..3126
                     /locus_tag="VKU83_03825"
     CDS             1309..3126
                     /locus_tag="VKU83_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HLI92705.1"
                     /translation="MNFRRFLTSLFLTMAMVVSHQCLARTAGRTQAATGSWIRVNELG
                     YPSSAVKVAVLASKTTIYSGHFELIDAATGKTVFKAAASHSFGPYGPFADTRRLDFSA
                     WRNEGTYYLRSGSARSPVFRIAKDVYSRSADFCLRYMRQQRSGFNPFLKDSCHTHDGY
                     TLYAPVPDSTYIDVVGGWHDASDYLQYSATSANAVYHLLAAWRDFPGVFRDRYQANGL
                     DGSNGQPDVLDEARWGLNWLLKMHPRPDWLFNQVGDDRDHRGMRLPKEDTNFYGRGLE
                     RPVYFCSGRPQVRGKFMNNTTGVASTAGKFAAAFALGARLYATIDSSYASRLRGKALT
                     ALQLGLDKPGVCQTASVLSPYIYAEDNWTDDMELAAASLGRLDLAYQYAQAEPVTPWL
                     GKDTANHYEWYPFINLGHDALARRLHDKRRDTVIRYYKEGIERIWRRASTNGFYRGIP
                     FIWCSNNLTVAFCVQCFWYRQLSGDRTYMPLEQACFDWLFGCNPWGVSMVCGLGPDGP
                     TDPHSAFTHIGHYPIDGGLVDGPVYTSIFTHLIGIRLSHPDAYAAFQSDLAVYHDDYG
                     DYSTNEPTMDGTASLIYLLAAHESEAEQPPRPKPRGSRP"
     gene            complement(3189..>3388)
                     /locus_tag="VKU83_03830"
     CDS             complement(3189..>3388)
                     /locus_tag="VKU83_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92706.1"
                     /translation="AIVIAVPVSWWAMRDWLQGFAYRVEIGAGVFLLAAFAVLLITVL
                     TIGFQALRAALANPVMTLRSE"
BASE COUNT          697 a         1113 c          880 g          698 t
ORIGIN      
        1 gtcacataca gcatcccgcc cgccaggcag acgccgccgg gataggcttc cgtggggata
       61 taccctttca ccgccgttcg cccgttaccg cccgtcgcca ttccggcgcc gagccggatc
      121 tcgcacacgg cattgtcaaa cccattcgcc acatacaagc gggtcccgct gctatccact
      181 gccagcgcgt cgggactact cccataatag gtcaaactcc ccgaaaaaag accgacaggg
      241 atcgaatcaa cgaccgcctc gcggctgaca ttgaagacgc tgacatagtc gctgttgcca
      301 ttgcagatat aaagaaatgc gctgtccgga ccagcggcaa tcgcattggg atgcaatcca
      361 agcgagacct cattcagcag gcggcctgtc cctgcgtcga taatcgacag acttccgcgc
      421 gcagttgcac cggttaccgg actcgtatac gcgcttcccc aaggagtgcc cgcatgctcc
      481 agcgtgctat ccgtcaccag gggcccggcc cagttcgtca catacgcttt cccgccaatg
      541 acgcgcagcc cataaggcgc tactccggtc gctgccgtcc agacgatctg gcggtcggca
      601 aaccggatct tcagcaattg attatttcca ttgagcacca cgtatacata cggctgaccg
      661 tcctccgtgc gcacggcgat atcattcggc aaagcggccg ccgcaggcgg cgccgcgttc
      721 agcggcacgc tgctaacttt tccaaagccc gccgcactcc attcggcgat catcaatgcg
      781 gaagaatgcg tgctccccgc gccccagaca atataggtct ggccgtcata cccgaaagac
      841 ttgatgccgc aataggtgct catcaaaccg tgcaccgcct gttcggtgcc ttccctgaac
      901 gtccagcggt ggacgattgt ctccgagcgc atatccaata ccgcgacccc gtatctgtcc
      961 tcaaccgcga tatgccgccc gtccggcaaa atggagaagt ccagggcatg attctcctgc
     1021 ctgggatcgc caaagctgac aacctttccc gcggaatgga tcagccggtt atacggcatc
     1081 atggtcaaac cgctttgctc catgagcgtg ctgtcatcca ttggcgttct gaacgatcca
     1141 tgccgttgcg cggttaccga tgtgcacacc aggcaaataa gagccgaaaa agttaatctg
     1201 ttagtcatgc caccaaatta cgcaagtttt cttttcctgt gattacctcc ctgttaagtc
     1261 ctgcggtatc cttttcaaat tttagccaaa aaattctaca ttcaccatgt gaacttccgc
     1321 cgcttcctga cctcgctgtt cttaacgatg gcgatggtcg tcagccatca gtgcctcgcg
     1381 cggaccgcag ggcgcacgca agccgccacc ggatcctgga tccgcgtcaa cgaactcggt
     1441 tacccttcct cagccgtcaa agtcgccgta ctggcaagca agaccacgat ttattccggt
     1501 catttcgaac tcatcgatgc agccaccgga aagaccgtat tcaaggctgc cgcctcccac
     1561 tccttcggtc cctatgggcc cttcgcggat acccgtcggc tcgacttctc cgcctggcga
     1621 aacgaaggaa cctattatct gcgctccggt tcagcccgtt ctcctgtttt caggatcgca
     1681 aaagacgtct actcccgttc cgccgacttc tgccttcgct atatgcgcca gcaacggagc
     1741 ggtttcaacc catttttgaa ggactcttgt catacgcatg acggttatac gctctatgcc
     1801 ccggttcccg acagcaccta catcgacgtc gtgggcggct ggcacgacgc cagcgactat
     1861 ctgcagtatt ccgccacctc cgccaacgcc gtttaccatt tgctggccgc ctggcgggac
     1921 ttccccggtg tgttccgtga caggtaccag gccaatggat tggacggatc caacggccag
     1981 cccgacgtcc tggacgaagc gaggtgggga ttgaactggc ttctcaagat gcatccccgt
     2041 ccggactggc tgttcaacca ggtgggcgat gaccgcgacc atcggggaat gcgactgccg
     2101 aaagaggata ccaactttta cggccgcggc ctggaacggc ccgtttattt ttgttccggg
     2161 cggccccagg tgcgtggaaa gtttatgaac aataccaccg gggtcgcttc aactgccggg
     2221 aaatttgccg ccgcgtttgc tctcggcgcc cgcttgtacg cgacgatcga cagcagttac
     2281 gcctcgcgcc tgcggggcaa agcgcttacc gccctgcagc tgggcctcga caaaccgggc
     2341 gtctgccaga cggcgtctgt cctgtcgccc tatatctatg cggaagacaa ctggaccgac
     2401 gatatggaac tggccgctgc ctctctcggc aggcttgacc tcgcttatca gtatgcgcag
     2461 gctgaacccg tcaccccctg gctcggaaag gatactgcca accattacga gtggtatcct
     2521 tttatcaacc ttgggcatga tgccctggcg cgccgcctgc acgacaaacg ccgcgacacc
     2581 gtcatccgtt actacaagga aggcatcgag cggatctggc gtcgcgcaag caccaacggg
     2641 ttttaccgcg gcattccttt tatctggtgc agcaacaacc tgacggtggc gttctgtgtg
     2701 cagtgttttt ggtaccggca gctgtccggg gaccggactt atatgcctct cgagcaagcc
     2761 tgcttcgatt ggctcttcgg ctgcaatcca tggggcgtat cgatggtttg tggtctgggg
     2821 ccggacggcc cgaccgatcc ccactcggct ttcacccata tcggccatta tccgatcgac
     2881 ggcggcctcg tcgacggtcc cgtgtacacc agcatcttca ctcacctgat cggcattcgc
     2941 ctctcccatc cggatgccta cgcggccttt cagagcgatc tcgcagttta ccacgacgac
     3001 tatggggatt acagcaccaa tgaacccacg atggatggca ccgcatcgct tatctacctg
     3061 ctcgcagccc acgaatctga agcggaacag cccccgcgac ccaagccgcg cggcagtcgg
     3121 ccgtgagcga atcgaatagc cacgtcgtta tgcggaatgg tgactgcacg ccaccgggtc
     3181 acccatggct attctgacct cagtgtcatc acaggattcg ccagcgcggc acgcagagcc
     3241 tggaagccaa tggtcagcac cgtgatcaac aagacggcga aagccgccag gaggaagaca
     3301 ccggccccga tctccacgcg ataggcaaaa ccctgcaacc aatctctcat cgcccaccag
     3361 gaaaccggga cggctatgac tatcgcga
//
LOCUS       DATGKE010000221         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1273491, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000221 DATGKE010000000
VERSION     DATGKE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273491"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..5231)
                     /locus_tag="VKU83_03835"
     CDS             complement(48..5231)
                     /locus_tag="VKU83_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HLI92707.1"
                     /translation="MKTSASIIFLVAAFISVTGPQDATAQRAMNPDDSVYTYNAAAAP
                     GTATNPNQPASGKIGKWVRTVRLSWNTNEYKAYIYNGSAFRIHFPKSYNPTAIDGKRY
                     PILIFYCGDGEEAGIYDNEDQLAHGGQPFMWAIDGGKFDGYAIFMQTRYGWGEAQWLN
                     YQALIDSLVVGYKGDPYRVIQNGLSGGGQGIWNHLAFNPTYFAASVPMSATLTSDGSW
                     SFIHSVRFMPIWNLDGALDNDPTPSLAFAIQDSMLKYGANYTLYEFPTEGHDTWDSTW
                     LKPGWWPYCNNSYQSNPWPLYGRTNFCPGQTIHATLGVVGGLQGYQWRKNGVNIAGAN
                     SDTLLVTAPGTYDVQVKRGNYYWSDFSHTPVVITVQSATQAPPVTTLNGASAVLPDAA
                     GDTSVTLTMPPGDSIYTWKYLRTGAIVGRSQSLTVGESAAGDYAALIIPPQGCSGIYS
                     PAFPVVKASGPNAPPPATNLEAAGQSFTSVRLTWVAIPNPVHKQTAFEIYRGSRSGTY
                     KFLRAVNPDSLGYTDIALQPGTKYFYVVRAVDTSGAAALSNEASATTLGDFTPPTAPG
                     NLTVVSTTSSSIALSWTASTDNVGVDHYAVYENGVLANVTHNLNFLVNALTAGSLYSF
                     YVKAVDGSGNYSNESNQVDAPAVNTGLNYSYYTTRTPWSVLPAFSALTPVKTGQMSNV
                     SIVNASQPFNFSYVWSGYITIPVTGTYTFATTSDDGSALWFGQLKPGSTSTATVNNDG
                     SHAATTVSSKAISLTAGQYPICVAYFQAAGGASMTVSWSSAQAFGNTTLRPIASQYFA
                     GGSSSGGAAPAKPTQVTAVASSYDRVNLTWKDNSNNETGFGIYRATAPTGPWTMVHMS
                     AAGSTSYSDAEGLLPNTRYYYKVAAVNQYGNSGYDSASMGGISYNFYKGSFNPLYNFD
                     SAHTPVVSSGVLNNISLSPAGSVTTYFGFLFAGTIRIPKSGTWTFYTRSDDASELFVN
                     GYKWANVVVNNNYQQAMTQRSGTITLAAGTYPLYVSYEQSAGGEGLTVSWSNTGAGIA
                     QSAIPDSAFGSAGWTTKTFAMPVALTKPDKVPVLNPIADIHLNFNQSATVTVTTTADS
                     TNAVTLTAANLPPFATFKDNGNGTGTISVRPSAGTVGSFLNITLSSADQNDSVSTATF
                     GIYVIDPNVTSTYIHFSDGIQLGQFPWNNFTSWPGAGYSLQKLVNDQSQSTEMSIAFT
                     NGMAGNFAGGMQPRNGAGIYPDAVMRSGEFENTSRVDTILVSGLTNSKTYNFVFFNSI
                     DYGGKGVTNYTINGTTVSLDPKYNLSNTARINHIMPNAAGQVRIAVSKANSNQSYAFI
                     NDLVVESYVDTPAKILSPVGLTVSDMSRNSVSLQWQVRSYNQTAIEVWRGTDSGGSAY
                     RLIATLPSAATAFTDATVRSNVNYFYLVRAVNANAASNFCNPVEATPYAYEVYMNFSV
                     LSAGLPWNNLLYVPNQGFVWPDNFFSDDIGAVTNTGLIETGLWAGEANFGMNTGNNSG
                     VVPDAVMQDAYAVFPGQTGSVRITGLDMDLTYDLTVFGSSQFSEDQNALYTANGKSCL
                     LNALNNETGELTIYGVTPDATGSINLSATCGTPTSRYAILNSVVIKGHNPYMPGATPA
                     RPAGTAMAGGNAVQGTAPVLAGTADSTLSGKDPIIYPNPVKSYFTLCVPATAPGKVQV
                     SIFDINGQLIYRREFGGLVSGNNFFEILTPPGMIPRGVYFVSVLYVDNKTVKAFKILK
                     D"
BASE COUNT         1205 a         1523 c         1631 g         1315 t
ORIGIN      
        1 gggaagtgat attggcgcag gaataaggga tctgccggac ctgcccccta atccttcagg
       61 atcttgaagg cctttactgt tttattatcg acatagagca cgctgacgaa gtaaacgcct
      121 cggggtatca tgcccggggg ggtcaggatc tcgaagaagt tgttacctga aaccagacct
      181 ccgaactccc tgcgataaat cagctgaccg ttgatatcaa agattgaaac ctgcacctta
      241 cccggcgccg ttgccggaac acaaagcgta aaatagctct tgaccggatt cggataaatg
      301 atcgggtcct tcccggaaag ggtgctatca gccgtgcccg ccagcaccgg ggctgttccc
      361 tgcacagcgt taccgccggc cattgcggtt ccggctggcc gggccggcgt agctccgggc
      421 atgtacgggt tatggccctt gatcaccacc gaattgagaa tggcataccg ggatgtcggc
      481 gtgccgcagg tagcggagag attaatgctt cccgtcgcgt ctggtgtgac gccgtagatg
      541 gtcaattccc cggtttcgtt attgagcgcg tttagcagac atgacttgcc attcgcagta
      601 tacagggcat tttgatcctc cgagaattga ctgcttccga agaccgtcag gtcatacgtg
      661 aggtccatat ccaggccggt gatccgcacg ctaccggtct gtcctggaaa aacggcatag
      721 gcatcttgca tcaccgcatc tggcaccaca cccgagttat tgccggtgtt cattccgaaa
      781 ttggcttccc ctgcccagag gccggtttcg atgaggcctg tattggtgac cgcacctatg
      841 tcatctgaaa agaagttgtc cggccataca aaaccctggt tcggcacata cagcaggtta
      901 ttccatggaa gacccgcgct gagtacggag aaattcatgt acacttcata agcatatggg
      961 gttgcctcta ccgggttgca aaagtttgac gccgcatttg cgttcaccgc tctgaccaga
     1021 taaaaataat ttacattgga cctcacggtc gcatcggtaa atgcggtggc ggcggaaggc
     1081 agggtcgcaa tcaaacggta ggcgctgccg ccactgtccg tgccgcgcca tacctcgatc
     1141 gctgtctgat tgtacgatct cacctgccat tggaggctaa ctgagttgcg gctcatgtcc
     1201 gataccgtca ggccaaccgg cgagagtatt ttggccggcg tgtcgacgta gctttccacg
     1261 accaggtcat taataaatgc atacgattgg ttgctgttcg ccttcgaaac agctatcctg
     1321 acttgtcccg cggcgttcgg catgatatga ttgatccggg ccgtattgct taaattgtat
     1381 ttggggtcca gactgacggt ggtaccgttg atggtgtagt tggtcacgcc tttgccgcca
     1441 taatcgatgc tgttgaaaaa tacaaaattg tacgttttcg agttggtcag gcccgagaca
     1501 aggatggtat ccaccctgct agtgttctcg aactcaccgc tccgcatgac cgcatccggg
     1561 taaatgccgg ccccgttgcg tggttgcatc ccgcccgcaa aattgccagc catgccattg
     1621 gtgaacgcta tgctcatttc ggttgactgg ctctgatcgt tgaccagctt ctgaaggctg
     1681 tagcccgcgc ccggccacga ggtaaagttg ttccacggaa actggcccag ctgaatgcca
     1741 tccgagaagt ggatataggt cgaagttacg ttcggatcga tgacatagat gccgaacgtc
     1801 gccgtgctca ccgagtcgtt ctggtcggcg gaggaaaggg taatgttgag gaagcttccc
     1861 accgtgccgg cggatggcct gacgctgatc gtacccgtgc cgttgccgtt gtctttaaag
     1921 gtcgcgaagg gcggcaggtt ggctgcggtc agcgtaactg cattggtgct gtctgcagtt
     1981 gtcgtaacgg tcaccgttgc cgattgattg aagttgaggt ggatatccgc gatcgggttg
     2041 agcacgggga ccttgtccgg ctttgtcagc gcaacgggca tagcaaacgt tttcgtcgtc
     2101 cagcctgcac tcccaaaggc cgaatccgga atagccgatt gcgcgatccc tgcgcccgtg
     2161 ttggaccaac tgaccgtaag cccctctccc ccggcggatt gctcgtacga aacatataat
     2221 ggatacgtgc ccgcggccag cgtgatggtg ccggaccgtt gcgtcattgc ctgctgataa
     2281 ttattgttga ccacgacatt tgcccactta tagccgttta cgaagagttc gcttgcatcg
     2341 tcggaacggg tgtagaaggt ccaggtgccc gatttcggaa tacggatcgt accggcgaac
     2401 aggaatccaa aataggtggt tacactgccg gccggtgaaa gggagatatt gttgagcaca
     2461 cctgaggaaa cgaccggcgt atgtgccgag tcaaagttgt acagcgggtt aaagctgccc
     2521 ttatagaaat tataactgat tccgcccatc gatgcactgt catagccgga attgccgtat
     2581 tgattgacgg ccgcaacctt ataatagtac cgggtattgg gcaatagtcc ttccgcgtcc
     2641 gaatagctgg tactgccggc tgccgacata tgaaccatcg tccacggtcc ggtcggagct
     2701 gttgcgcgat agatcccgaa gcccgtttca ttattgctgt tatccttcca ggtcaagttg
     2761 acgcggtcgt acgagctggc cacggccgtg acctgtgtcg gcttagccgg ggcggcgcct
     2821 cccgacgaag aaccaccggc gaaatactgg cttgcaatcg gccgaagcgt cgtatttcca
     2881 aacgcctgtg cactcgacca ggacactgtc atgctggccc ctccggcggc ctggaaatac
     2941 gcgacgcaga tcggatattg acccgcagtc aggcttattg ccttgctgct gaccgtagtc
     3001 gctgcatgcg agccgtcgtt gttgaccgtg gcagtggacg tgctgcccgg cttgagttgg
     3061 ccgaaccaca aagcgcttcc gtcatcggaa gtagtggcga aagtgtaagt tcccgtcacc
     3121 ggaatcgtga tatacccgct ccagacgtaa gaaaaattga agggctggct cgcgttgaca
     3181 atgcttacat tggacatttg tcctgtttta accggtgtca gcgccgaaaa ggcgggcaag
     3241 acgctccatg gcgttcgggt ggtgtagtag ctgtagttca agccggtgtt tactgcgggc
     3301 gcatctacct ggttgctttc gttcgaatag ttgccgctgc cgtcgaccgc ctttacatag
     3361 aaggaataaa ggctgccggc agtgagcgca tttacgagga aattcagatt gtgggtcaca
     3421 tttgctaata cgccgttttc atacacggca tagtggtcga cgcccacatt atccgtcgat
     3481 gccgtccagg atagtgcgat cgaactgctg gtcgtcgaca caacggtaag atttccgggc
     3541 gctgtcggcg gtgtgaagtc tcccagcgta gtggcggaag cctcgttcga cagtgctgcg
     3601 gcgccgctgg tgtcgacagc gcgaacgaca tagaagtatt tggtacccgg ctgcagtgcg
     3661 atatccgtgt aaccaagcga atccggatta acagcccgca aaaatttgta ggttccgctt
     3721 ctggatccgc ggtagatctc gaatgctgtc tgtttgtgga ctggatttgg aatggcgacc
     3781 caggtcagcc gcaccgacgt gaagctctgg ccggcggctt ccagattcgt tgcgggcggc
     3841 ggcgcgttcg ggcccgatgc cttgacaaca ggaaatgcgg gcgagtagat cccggaacag
     3901 ccctggggag ggatgatcag cgcggcgtaa tcgccggcag cgctttcgcc gacggtaaga
     3961 ctttgactgc ggccgacgat tgctcccgtt cgcagatatt tccacgtgta aatggaatcg
     4021 ccaggcggca tggtcagcgt caccgaggtg tcccccgccg cgtccggtaa tacggcgctc
     4081 gccccgttga gtgtggtcac cggcggcgct tgcgtcgccg attgaaccgt gatgactacc
     4141 ggcgtgtgtg aaaagtcgga ccaataataa ttgccgcgtt tcacctgcac atcgtaggta
     4201 ccgggcgccg taacgagcag cgtatcggag ttggctcccg cgatattcac gccgttcttg
     4261 cgccactgat atccctgaag tccgccgact acgcccagcg tcgcatggat cgtttgtccc
     4321 ggacaaaaat tcgtccggcc atataagggc cagggattag actggtagga attgttacaa
     4381 tatggccacc agcccggttt aagccaggtg ctgtcccagg tatcgtggcc ttcggttgga
     4441 aattcgtaca gggtgtaatt ggcgccgtat ttcaacatcg agtcctgaat ggcgaacgcg
     4501 agcgagggag ttgggtcgtt atccagcgcc ccatcgagat tccagatggg catgaatcga
     4561 accgaatgga taaaggacca tgagccgtcg cttgtcagcg tggccgacat cggtaccgag
     4621 gccgcgaaat aggtcgggtt gaacgcaagg tggttccaaa tgccctggcc gccgccggag
     4681 agtccatttt gtatcacccg atacgggtcg cctttatagc caacgaccag tgaatcgatg
     4741 agcgcctggt aattcagcca ctgcgcctcg ccccagccat accgagtttg catgaatatc
     4801 gcataaccgt cgaacttgcc gccatcaatt gcccacatga aaggctggcc gccgtgggca
     4861 agctgatcct cgttgtcata gatacctgcc tcttcgccat cgccgcaata gaatataagg
     4921 atcggataac gtttgccgtc aatggcggtc ggattatatg acttggggaa gtggatccga
     4981 aaagccgatc cgttatagat ataggccttg tattcgttgg tgttccatga cagccggacg
     5041 gtccgaaccc acttcccgat cttgccggat gcgggctgat tcggattcgt agcggttcct
     5101 ggtgctgcgg cggcattgta ggtgtacacc gaatcgtcag ggttcatcgc acgctgtgcg
     5161 gttgcgtcct gcggtcccgt cacagaaata aaagctgcta caaggaaaat gatgctggca
     5221 gaagttttca tgattctgaa ggttgaggca acaccatcag gatcggaatt agcggcaagt
     5281 cagaccgaaa aaagcgactg gattatcagc gcaattagac cgcgcggaaa gactacgaga
     5341 aaatccgaag taaagatact ggattctccc tcatggcgaa attttttccg gtcgaatatc
     5401 ctggcctaac gggcaacatt cagtgctcgc cggagtttcc gggattcgtt ccagcgtcag
     5461 gcccctccgc tcttttgcgg ttaataaagt cctatttttg cggctcatca tatcttcgga
     5521 tgccacagat cctattattc ggtgcgggca aatcggccac cagcctgatc cggtatttga
     5581 ttgcggttgc cggccgccgc gactggcggc ttactgtcgc ggaaaccaat cttgcgctgg
     5641 cttcatccaa ggtcggcccg gcgtcctgtg cgca
//
LOCUS       DATGKE010000222         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_749563, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000222 DATGKE010000000
VERSION     DATGKE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749563"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..710)
                     /locus_tag="VKU83_03840"
     CDS             complement(51..710)
                     /locus_tag="VKU83_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92708.1"
                     /translation="MKRIAAILVWGLLLWGIGTFLDQVRSMLMMGGDPVKTYIVRFGF
                     GFGLVIIVVLAGRWFGPRMLGTGDWFMAGVVAWVVQGGYGWTRATTTGAGPTFDINVH
                     DTYFAVATSHLIAGIVLLYMLLGLIYYMSRGMNRILGNVHFFVTHAALFVLFWMQYFN
                     PAYQVKGYLEWHEYYQSFVVVDWAYAGMAILLVAVQVLFLVNLLLMAFSRARQRQDRR
                     F"
     gene            complement(737..1603)
                     /locus_tag="VKU83_03845"
     CDS             complement(737..1603)
                     /locus_tag="VKU83_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI92709.1"
                     /translation="MKPIHIYAVILITGCSSCVSVGAYNALQKDKLKSDSLYGWAMQS
                     LKSCQVDNDRLTRQRSSIQDQNKELNLQMTAISDNNTALRKQLSDLSAISSAQAESIR
                     KSIDNIGAKDLYLQGLRTAISRRDSINLAALMETKAAMGSFSDREVTIKVAKGVMDVD
                     VSDSVLFGGDSAGYAVTPKGKTVLLRLARVLNDQTDIRCTVEAYTDSASAGQDSVMDS
                     WELSVRRAAAVVRLLQNQFNVMPSRLVAAGRGEYEPVAPNDSPEDRAANRRTRFRISP
                     QNNELEEALEKR"
     gene            complement(1724..2158)
                     /locus_tag="VKU83_03850"
     CDS             complement(1724..2158)
                     /locus_tag="VKU83_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92710.1"
                     /translation="MKKIIFVSLFILPFMSRAQFTITNSSTTSLLEVREGVWPMELQR
                     IIKDLDTCYVLEFRDQQFSTVVNMTTLRFGNMQQLKYFMLGLATLKKGNTGDIAHFKN
                     YTIKRVDVKNTGVWYLLSLSDGGVTNFQQSDADKMIAAIKVL"
     gene            complement(2212..2643)
                     /locus_tag="VKU83_03855"
     CDS             complement(2212..2643)
                     /locus_tag="VKU83_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92711.1"
                     /translation="MKKRCLSFLLFPFTAFSWLSLDPTSINLADVRTGEWPIRLEKDI
                     DDQGATYSLIFRDQQLETAVVMDTLVFQDLAKLRYFAQALEVLKKGHNGDIANFKNFT
                     LKRADKRFDGVWYMLRIKYGLTDFQQPEADIMCKTIDALKQ"
BASE COUNT          669 a          819 c          642 g          663 t
ORIGIN      
        1 attgcatatt atttggtttt tgttcgctat ttccatctac atcccaacga tcagaaccgc
       61 cggtcttgcc gttgcctggc ccggctgaac gccatcagga gaaggttcac cagaaacaag
      121 acctggactg cgacgagcaa tatcgccatg cccgcgtagg cccagtccac taccacgaag
      181 ctctgataat attcgtgcca ttcgaggtag ccctttacct ggtaagccgg gttgaaatat
      241 tgcatccaga acagcacgaa cagggccgca tgggtcacga aaaaatgtac attcccaaga
      301 atgcggttca tgccgcggct catatagtaa ataagcccca acagcatgta caacaggaca
      361 atgccggcga taagatgcga agtggcaacg gcaaaatagg tgtcatggac attaatgtcg
      421 aaggttggac cggctccggt cgtcgtcgcc ctcgtccagc catagccgcc ctgcaccacc
      481 cacgccacaa cccctgccat aaaccagtcc cccgtcccca acatccgcgg cccgaaccat
      541 ctgccggcca gcaccacgat aatgaccagc ccgaacccaa acccaaaacg gacaatatac
      601 gtcttaaccg gatctcctcc catcatcaac atcgaccgca cctggtccag gaaggtcccg
      661 atgccccata gcagcagccc ccaaaccaaa atcgccgcga tccttttcat gttatcgttt
      721 ttggcgcccc cggcacctac cgcttttcga gcgcttcctc aagttcgttg ttttgggggc
      781 tgatccggaa tctcgtccgc ctgttcgctg cgcgatcctc cggactatca ttcggcgcca
      841 ccggctcgta ctccccacgt cccgccgcaa cgagccgcga gggcatgaca ttgaactgat
      901 tctgcagcaa tcgtactacc gctgccgccc gtcgcacgct cagctcccag ctgtccatta
      961 ccgagtcttg cccggctgag gcactgtcgg tatatgcttc caccgtgcaa cggatgtccg
     1021 tctggtcgtt taatacccgt gccagccgga gcaacactgt ctttcctttc ggggtgacgg
     1081 catagccggc gctgtcaccg ccaaataaaa cgctgtcgga aacatcaaca tccatcacgc
     1141 cttttgcgac cttgatagtt acttccctgt cggaaaaact gcccatcgca gctttcgttt
     1201 ccatcaacgc tgccaggttg atcgagtccc gccgcgagat cgctgtccgc agcccctgca
     1261 ggtagaggtc cttggcgcca atattgtcga tggatttcct gatgctctcc gcctgcgcgc
     1321 tgctgattgc cgaaaggtcg ctgagctgct tccgaagcgc cgtattgttg tcggagatgg
     1381 cggtcatttg caggttcagc tctttgttct gatcctgaat gctgctcctt tgcctggtga
     1441 gccgatcgtt gtcgacctga cagctcttta gggactgcat ggcccagcca tacagggaat
     1501 cactcttgag cttgtccttt tgcaaggcgt tgtatgcacc aacgctgaca caggaactgc
     1561 aaccagtaat aagaataacg gcgtaaatat ggattggctt catagctgca ccaaagatat
     1621 actttctttg ctaccgaaga cctctccgcc ccccgccagg tgtaccgggc ggccgaaaaa
     1681 aaaccgcttc ctgggaagcg gccatataaa atctttcgtt ttcctacaaa acctttatcg
     1741 ccgcgatcat cttatcggca tcgctctgct gaaaattcgt cactccccca tcgctcagac
     1801 tcagcaaata ccatacgccg gtgttcttca catcaacccg cttgatcgta taattcttga
     1861 aatgggcgat atcccccgta ttgcctttct tcaacgtcgc cagacccagc ataaaatatt
     1921 ttaactgttg catattccca aaccgcaggg tcgtcatatt cacaaccgtc gaaaattgct
     1981 gatcgcggaa ctccaacacg tagcaggtat ccaaatcttt aatgatccgc tgcagctcca
     2041 tcggccatac cccctcgcga acctccagca ggctcgtcgt ggacgaattg gtaatggtga
     2101 attgcgccct ggacataaaa gggaggatga ataaggatac aaaaatgatt tttttcatac
     2161 cggaaatttt cttttcgtgt tacaatctta tagaacgggg aagcggccga tttattgttt
     2221 caatgcatca atagttttgc acataatgtc ggcctccggc tgttgaaaat cggtgaggcc
     2281 gtatttgatc cgcagcatgt accagacgcc atcaaagcgc ttgtcagcac gcttgagggt
     2341 gaagttcttg aaatttgcaa tatctccgtt atgccccttt ttcagcacct ccaaagcctg
     2401 ggcgaaatat cgcagcttgg ccaggtcctg gaaaacaagg gtatccatca ctaccgcggt
     2461 ctcgagctgt tgatcccgaa atatcaggga atacgtcgcg ccctggtcat cgatatcctt
     2521 ttcgagacgg atcggccatt cgcctgtcct gacatccgcg agattgatgc tggtgggatc
     2581 caatgacagc caggaaaaag cggtgaaggg gaagagtaga aagctaagac atcgtttttt
     2641 catagatata gaatagctca tccaatatag gcaactaaat ttataaatgc aaaagccata
     2701 cttttatgcc gtcataattt aacatttatg caactcaccg gaaacaagat cctggttacc
     2761 ggcggcggta ccgggatcgg ccgcgggctc acc
//
LOCUS       DATGKE010000223         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_855783, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000223 DATGKE010000000
VERSION     DATGKE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855783"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VKU83_03860"
     CDS             complement(<1..569)
                     /locus_tag="VKU83_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92712.1"
                     /translation="MRHPTPLLLVFFTMLMFPLIAGAIDPHDTRMLFQPAISADQIAF
                     IYARDLWVAKLDGSQPRRLTVSEGLESNPVFSPDGRLIAFSAEYDGNVDVYVIPAEGG
                     IPKRLTWHPAQDIVQGFSPDGKSVLFASPRNTFSNRYLKLFTVSVDGGPEQELEIPNA
                     YWATYSPDGGRIAYTPIPDAFRQWKHYRGG"
     gene            656..1846
                     /locus_tag="VKU83_03865"
     CDS             656..1846
                     /locus_tag="VKU83_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLI92713.1"
                     /translation="MRLPVSPSPFLLDPTITYLNFGSFGACPRPVFEDYQRWQRELEL
                     NPARFMNVTGPCYLEEARQALAAYVHCGAEDIVYVTNPSYAVNIIAKSFRLSPGDEIL
                     STDLEYGACDLTWNYYCKKNGAAYIRRPIRLPLTSREEFVQDLLDGMTSRTKAIFISH
                     LTSATALILPVEEICEVARQKGIVSIVDGAHAPGQLPLDLTRFRPDIYVGACHKWMMT
                     PKGSAFLYVRKELQPYFDPLLISWGYGGETASGSRFLDYHQGQGTRDFSAFLTIPAAI
                     RFMEDHQWTAVTAACRSLVRENAGRFADLLGATLLCPVGGTFLGQMLSIPINTPDPEK
                     LKRRLFDDYRIEIAVNKQERRTLLRYSVNGFNGEEDLDRLYSALQEIAAENHLLVRGA
                     ALTA"
     gene            complement(1862..2443)
                     /locus_tag="VKU83_03870"
     CDS             complement(1862..2443)
                     /locus_tag="VKU83_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92714.1"
                     /translation="MKMVSKLVVVLLLLGGPARAQVDIPRMDSTLKIGKAGYRVECRN
                     RKIDVNQLDIRPVGFDNQAKPVSFLLRGRVAKAEIDDLNGDGYPDLVLYVYTDSAAIY
                     GTVYAFVSKGNKSIAGCTLPDPMMDGHISSGYRGHDQFSLLQGYLLEKFPIYKTGDAA
                     DKPTGGTRAILYQLVKGGKDEFEFQQVRHYDTN"
     gene            2513..>3209
                     /locus_tag="VKU83_03875"
     CDS             2513..>3209
                     /locus_tag="VKU83_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92715.1"
                     /translation="MRLIPKPRIVVHRKIAWLLVFSILSLMVVGYLIYATKRSMGQTN
                     FRINRTYEAISTLQRLNLLATESGSEAYRYAQTADGQDAQDLTRSHAGLMAFLDSLQQ
                     LTAGNCDQDSSISRLSGLIDTKCRAEDSLVANKTSPEPGRLASADRAVKQLLESMTLT
                     ERRLLTDRMQLNEDENRRTAEIALIARIAACFFVVFILIKINGDISHRKKAQDRLEIA
                     IREARDAKLMQEQF"
BASE COUNT          707 a          968 c          853 g          681 t
ORIGIN      
        1 ccgccgcgat aatgtttcca ttgccggaat gcgtcgggga ttggcgtgta agcgatccgg
       61 ccgccatcgg gggaatacgt agcccagtat gcattcggga tctccagttc ctgctccggt
      121 ccgccgtcta cggaaaccgt gaacaatttc aggtaccggt tggagaaggt gttccgcggc
      181 gaagcgaaca gtaccgattt tccgtcaggg ctgaatccct gcacgatgtc ctgcgccggg
      241 tgccaggtca ggcgtttggg gataccccct tcagccggga tcacataaac gtccacattc
      301 ccatcgtatt ccgcgctgaa tgcaatcaac cggccatcag gcgaaaatac cgggttggac
      361 tccagtccct cgctcacggt caatcgccgc ggctgcgagc cgtccagctt cgcgacccaa
      421 aggtcccgcg cgtaaatgaa ggcgatttgg tcggcgctta tcgccggctg gaacaacatc
      481 cgggtgtcgt gcggatcgat ggcgcccgcg atcaacggaa acatcagcat agtgaaaaaa
      541 acaagcagta atggtgtggg atgacgcata aagtagatta tcccaaaaag atacccctat
      601 tttcccgaac ggccagccgg tttttagccc ccgccggaaa tcagtatttt tgcccatgcg
      661 actacccgtt tcaccttcgc ctttccttct cgacccgacg atcacctatc tcaacttcgg
      721 ctcattcggc gcttgtccgc ggccggtttt tgaagactac cagcgctggc agcgggaact
      781 tgaactcaat cccgcccggt ttatgaacgt aaccgggccc tgttacctgg aggaggcccg
      841 gcaagctctt gccgcttatg ttcactgcgg cgcagaggat atcgtctacg tcaccaatcc
      901 atcctatgcc gtcaatatta tcgcaaaaag tttcaggctt tcaccgggtg atgagatcct
      961 tagcaccgat ctcgaatatg gcgcctgcga cctgacgtgg aattattatt gtaagaagaa
     1021 cggcgcagcc tacatccgcc gtcccatccg cctgccgctc acgtcaaggg aggaattcgt
     1081 tcaggatctg cttgacggga tgaccagccg gacgaaagcc atttttataa gccacctgac
     1141 aagcgccacc gccctgatcc tgcccgtaga ggaaatctgc gaagttgccc ggcagaaagg
     1201 cattgtcagc atcgtggacg gggcccacgc gcccggtcag cttcccttgg atctcacccg
     1261 ttttaggccg gatatctatg tcggcgcctg ccataaatgg atgatgacgc cgaaaggcag
     1321 cgcttttcta tacgtccgca aggaactgca accttatttc gacccccttc tcatcagttg
     1381 gggctatggc ggtgaaactg cctccggatc gcgattcctg gactaccatc agggccaggg
     1441 cacgcgcgat ttctccgctt tcctgaccat tccagcggcg atccggttca tggaagacca
     1501 tcaatggacc gccgtgaccg ccgcctgccg tagcctggtc agggaaaatg ccggccgttt
     1561 cgccgatctg cttggcgcca ccctcctctg tcctgtcggg ggaacattcc ttggccagat
     1621 gttgagcatc cccataaata ctccggatcc ggaaaagctg aagcgccggc tattcgacga
     1681 ctaccggatc gaaatcgccg tcaacaaaca ggaacgccgg actttactgc ggtattccgt
     1741 taacgggttc aacggtgagg aggatctgga tcgcctctat tccgccttgc aggagatcgc
     1801 agcggagaat cacctgttgg tgcggggcgc ggcgttgacc gcatagcgat acccggacgc
     1861 gtcaattggt gtcgtaatgc cgcacctgct gaaactcgaa ctcgtccttg ccgcccttaa
     1921 caagctggta aagtatcgcc ctggtgccgc ccgtcggctt atccgcggca tctccggtct
     1981 tatagatggg aaacttttcg agcaggtatc cctggagcag cgagaattga tcatgaccgc
     2041 ggtatcccga actgatatgg ccatccatca tgggatccgg caacgtgcag cctgcaatgg
     2101 acttgttgcc tttggaaacg aatgcataga ccgtcccata aatcgcggct gaatcggtgt
     2161 agacatacag cacaaggtcg ggatacccgt ctccgttcaa atcgtcaatc tccgccttgg
     2221 ccacccttcc ccggagcaga aaactcaccg gcttggcctg gttgtcaaag ccgaccggcc
     2281 ggatatccag ttggttgacg tcgatcttcc tgttccggca ttcgacccgg tagccggcct
     2341 ttccgatctt gagagtggaa tccatcctgg gaatgtcgac ctgcgcccgg gccgggccgc
     2401 caagcagcag caatactacg accagtttac tcaccatttt catggcataa agataaagag
     2461 atacataata aattgacttt tttgcgttat agtgtgtcct gaagacaatg ctatgaggtt
     2521 gattcccaag cccagaatcg tcgtacatcg caagatcgcc tggcttttgg tattttccat
     2581 cctttccctg atggtcgtag gctatttgat ctatgccacc aagcggtcca tgggacagac
     2641 gaacttccgg atcaaccgca cctacgaagc gatcagcacg ctccagcgcc ttaatctcct
     2701 cgcaaccgaa tcgggctccg aagcataccg gtacgcgcag accgccgacg gtcaggatgc
     2761 gcaggacctt acccgctcac acgccggcct gatggcattc ctcgactccc tgcagcaact
     2821 taccgccggg aactgtgacc aggacagcag catcagccgg ttgtccggcc tgatcgacac
     2881 caaatgccgc gctgaagaca gcctggtcgc caacaagacg tcgccggaac ccggccggct
     2941 cgcatcggcg gaccgcgcgg ttaagcagct cctggaaagc atgaccctca ccgaaagaag
     3001 gctgctgact gaccgtatgc aactcaacga agacgaaaac cgccggacgg cggagattgc
     3061 cctgatagca aggatcgccg cctgcttctt cgtggtcttt attttgatca agatcaacgg
     3121 tgacatcagc caccgcaaaa aggcgcagga ccggctcgag atcgcgatcc gtgaagcgcg
     3181 cgacgcgaag ctgatgcaag agcagttcc
//
LOCUS       DATGKE010000224         5811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1476244, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000224 DATGKE010000000
VERSION     DATGKE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476244"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VKU83_03880"
     CDS             complement(<1..668)
                     /locus_tag="VKU83_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92716.1"
                     /translation="MIQSKDDPGYRQCAVTVMDNIADPDIRFDDKGICNYYYEYIQAE
                     KAGVLTGEAGEKMLRQLAGKIRAAGQGKAYDCLIGLSGGVDSTYVAYVIKQLGLRPLA
                     VHLDNGWNSELAVRNVENIVTKLNFDLYTLVVNWEEFKDIQLAYLRASVVDIEVVSDH
                     AIFATMYKLAREKKIGYIISGTNIVTEYIMPPSWLYHKMDFANLRDIHSRYGKMKLKT
                     YPTF"
     gene            complement(655..1446)
                     /locus_tag="VKU83_03885"
     CDS             complement(655..1446)
                     /locus_tag="VKU83_03885"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HLI92717.1"
                     /translation="MKRIRVIPALLIQKGGLVKSVRFKDQKYVGDPINAVRIFNEKEV
                     DEIVLLDISATAEKRPPNIRQIREIASEAFMPLGYGGGITNLGEIKELIAAGVEKVIL
                     NTSAFRNPGLVAEAAKYAGSQSIVVSMDVKKNLWGKYKVYVQNATMNTGSDPAEYAIR
                     MEKAGAGEILLNAVDRDGTFAGYDTDLIRMISSKVNIPVVAIGGAATVNDFSKAVLSG
                     ASAVSGGSMFVFQGPHRAVLISYPGQQELKDKFFSQLAQTTNDTK"
     gene            complement(1446..2063)
                     /gene="hisH"
                     /locus_tag="VKU83_03890"
     CDS             complement(1446..2063)
                     /gene="hisH"
                     /locus_tag="VKU83_03890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLI92718.1"
                     /translation="MLTIVDYGVGNLASIKNMLKKAGKEALISSDPDTVSQADKLILP
                     GVGAFDTCAEKLRQSGLTGILNKKVLEEGTPVLGVCVGMQLLMEGSEEGSQPGLGWIK
                     GRVIKFRQEKLPSGWKIPHMGWTDIIVKKPSPLFYGMYDEPRFYFVHSYHPDPADTAD
                     VLVEADYGYTFAAGLEHGNIAGVQFHPEKSHKFGLRLLSNFVSNY"
     gene            complement(2063..2890)
                     /locus_tag="VKU83_03895"
     CDS             complement(2063..2890)
                     /locus_tag="VKU83_03895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI92719.1"
                     /translation="MYDFEREYYEDESFWEGGMLEDAPNRERIRFTLKIIPDDVKSIA
                     DIGCGNGVFVNQLKASRPFLDIMAIDRSETALKYVQTGKRQGDISEIPLGDKSVDCAS
                     CLEVIEHLPVPVYERALDELARVSKEYIIISVPYKERLEESFNQCPSCGTIFNYELHL
                     RTFDDENVNSLLRSRGFSCIESHHLGEMTAYKGHYMFRKIFYREQFRQWKSPICPLCG
                     YKEHESHSEYPVREKSPGAQIGNRKRKLISYITRFPKAIWPKEKRYYWIIALYKRVS"
     gene            complement(2932..3750)
                     /locus_tag="VKU83_03900"
     CDS             complement(2932..3750)
                     /locus_tag="VKU83_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI92720.1"
                     /translation="MSATPLVSVLMTSYNREKYIAQAIESVLVSSYRNFELIIVDDCS
                     GDKTVEIAKGYAAKDERVKVYVNEKNLGDYPNRNKAASYARGKYIKYLDADDLIYYYG
                     LEVMINYMERFPSAGFGLASIVSNENPFPICIGPAAIYLENFDGFGHFSRAPGSSIIR
                     LDSFTKVGGFSGARMIGDFELWCKLGRYYDMVKFPFDLYWNRLHDEQESKSEYAKQYP
                     VLIEKVLSEALSHPECPLNPTELDTIKKMLSKQRIRGRALNVLSKMNRLIRPRK"
     gene            complement(3767..4564)
                     /locus_tag="VKU83_03905"
     CDS             complement(3767..4564)
                     /locus_tag="VKU83_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLI92721.1"
                     /translation="MAKIKQAAKSLLGDLNVYRMREWKNRILPSEFYRKEQADMVRRQ
                     AFYSQFVKAGDLCFDVGANLGNRVGPLLAAKARIVAVEPQAFCQKVLHWRFGKKIAVV
                     PMGLGETEEVREFYISDASYLSSFSKNWIDSVKNGRFKENEWTNAGKVQMTTLDKLIA
                     TYGTPAFIKIDVEGYELEVLKGLNAPVKMISFEYTTPEQTERAIACIERVRSVNPDID
                     CNYSVGESMEFEMPEFIKVEDMLRLVNSRKFSETGFGDIYLRSATRG"
     gene            complement(4539..5312)
                     /locus_tag="VKU83_03910"
     CDS             complement(4539..5312)
                     /locus_tag="VKU83_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLI92722.1"
                     /translation="MIKRLIKGLLNKAGMEVTLKKNLWYLDAFEVQKRIIGKAAPVIF
                     DAGASEGSTVTKYKSLYPAAAIHAFEPQPDSFQKLRATVSAMKEIYCSNVALSAIQGT
                     DIFYKTSGYAASSLLAPVRTGSFIDDHTGLAERYEVRLEKLDDYAKSRNIVHIDILKM
                     DTQGNELNILKGGEELLKRGAISLIYTEVWFIKAYEDQPFYEDIALYLRQFDYLPFGL
                     YNIHRDLRLNGKNLWADAIFVKGDNSLKLNIHGKNKAGS"
     gene            complement(5278..>5811)
                     /locus_tag="VKU83_03915"
     CDS             complement(5278..>5811)
                     /locus_tag="VKU83_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92723.1"
                     /translation="LSEGKTTYIDTCHNFYPYHYRFSWVNPIPAIADRIRGLEEKMKQ
                     RHCIGLHIRRTDNLASVENSPDFLFENAIREEICRDPSAVFFLATDDYRTQSHFTGLF
                     GPDRILAHPKKFGRDSVEAIRDAVVDWTLLTRCSKLYCSYYSSFSETVIEVSNAPAIT
                     LKIQPENDKKTDKGFAE"
BASE COUNT         1379 a         1581 c         1242 g         1609 t
ORIGIN      
        1 tcgaaggtcg gataggtctt caatttcatc ttcccatacc ggctgtgaat atcccgcagg
       61 ttcgcaaagt ccattttgtg gtaaagccag ctgggcggca tgatatactc cgtcacgata
      121 ttggtgccgc taatgatata accgattttt ttttccctgg ccagtttata catggttgcg
      181 aaaatggcat ggtccgagac cacttcgata tccacgacgg aggcgcggag gtaggcgagt
      241 tgaatgtcct tgaattcctc ccaattgaca accagcgtat acaggtcaaa attcagtttg
      301 gtaacgatat tttcgacatt cctgactgcc agctcggaat tccagccgtt gtccaggtga
      361 accgccagcg gacgcagtcc cagttgtttg atgacatagg caacatacgt gctgtccacg
      421 ccgccactca ggccgatcag gcaatcatac gcttttccct ggcccgccgc ccggatcttt
      481 cccgccagct gccggagcat tttttcgccg gcctcgcccg tcaggacccc ggccttttcc
      541 gcctggatgt attcgtagta atagttgcaa atgcccttat catcaaaccg gatatccggg
      601 tctgcaatat tatccattac cgttacggca cactgcctgt accccggatc gtctttactt
      661 tgtatcattt gtcgtctggg ctagttggct gaaaaattta tccttcaatt cctgttgacc
      721 gggatagctg atcaataccg cccggtgcgg cccctggaac acaaacatac tgccgcccga
      781 tacggccgaa gccccgctta agaccgcctt gctgaaatca ttgaccgtcg ccgcaccgcc
      841 gatagccacc actgggatat tcaccttact gctgatcatc cggatcagat ccgtgtcata
      901 accggcgaag gttccgtccc tgtccactgc gttcagtagg atctcgccgg ccccggcttt
      961 ttccattcga atggcgtact ccgcggggtc cgacccggta ttcattgtag cgttctgcac
     1021 gtagactttg tacttccccc agaggttctt tttaacgtcc atggaaacga cgatgctctg
     1081 gctgccggca tatttggccg cttccgctac caggccgggg ttcctgaatg cgcttgtgtt
     1141 caggatcacc ttctcgacac ctgcggcgat cagttccttg atctccccga gattcgtgat
     1201 cccgccgccg tacccgagcg gcataaaagc ctcactggcg atctccctta tttgccggat
     1261 attgggcggc cttttttccg cggtcgccga gatgtccagg aggacgatct catccacctc
     1321 cttttcgttg aatatcctga cggcattgat aggatcgccc acatatttct gatccttaaa
     1381 ccggaccgat ttgactagtc cgcctttttg gatcagcaac gccggtatga ccctgattct
     1441 cttcatcaat aattgctgac aaaattggac aaaagcctca acccgaattt gtggcttttt
     1501 tccggatgaa attgtacgcc ggcaatattg ccgtgctcca ggcctgctgc aaatgtatac
     1561 ccatagtccg cctccaccag cacgtccgcc gtatccgccg ggtcgggatg ataggaatgc
     1621 acaaagtaga accggggttc gtcatacatt ccgtaaaaaa ggggggaggg cttcttcacg
     1681 atgatgtctg tccagcccat atgcgggatc ttccaaccgg atgggagctt ttcctgcctg
     1741 aacttgatca cccggccctt gatccacccg agtccgggtt gagaaccttc ttcgcttccc
     1801 tccatcagca actgcatgcc cacgcatacc cccaggaccg gcgtcccttc ctccaggact
     1861 ttcttattga ggatgccggt caatcccgac tgcctgagct tttcggcgca ggtatcaaat
     1921 gcgccgaccc cgggcaggat aagcttatct gcctgtgaga cggtatccgg atccgaagag
     1981 ataagggctt cctttcccgc ctttttcagc atgttcttga tggaggcaag gttgcctacc
     2041 ccatagtcga cgatcgtcag cactagctta ctcttttata caaagcaatg atccaataat
     2101 accttttttc tttaggccag atagccttcg gaaaccgggt tatataagaa attaactttc
     2161 ttttcctatt tccgatttgt gccccgggag atttttccct tactgggtat tcggagtgac
     2221 tctcatgttc cttatatcca cacaaagggc atataggaga cttccactgc cgaaactgct
     2281 cgcgataaaa tatttttcga aacatatagt gccctttata ggcagtcatt tctcccaaat
     2341 gatgagattc aatacaggaa aagcccctcg atcgcaataa actgttcaca ttttcatcat
     2401 cgaacgttcg caaatgcagc tcatagttga aaattgttcc acaggaagga cattgattaa
     2461 agctttcttc taacctttcc ttgtaaggaa cactaatgat aatgtattct ttagacaccc
     2521 tggccaactc gtccaatgcc cgctcataga ccggcactgg caaatgctct attacttcaa
     2581 gacaggaagc acagtcaacc gatttatcac cgagaggaat ttccgaaata tcaccctgcc
     2641 tcttgcctgt ctggacatat tttaaggcgg tttcacttct gtcaatggcc ataatatcaa
     2701 gaaagggtct gcttgccttc aattgattga caaaaacacc gtttccacag ccaatatcag
     2761 caatgctttt tacgtcatcc ggaatgatct tcaaggtaaa acgaatcctt tcccgatttg
     2821 gggcgtcctc cagcatcccg ccttcccaaa aagattcgtc ttcataatat tccctttcga
     2881 aatcatacat atcaaacaat ttgattattt gatgagcgta tcatcgcatc attattttct
     2941 gggccttatt aacctattca tttttgataa gacgtttaga gcccttcctc gtattctttg
     3001 ctttgataac atttttttta ttgtgtcaag ctccgtgggg ttcaaaggac attcaggatg
     3061 gcttaatgcc tcacttagaa ctttctctat taaaacagga tattgttttg catattccga
     3121 tttagactcc tgctcatcat gcaatctgtt ccagtaaagg tcgaatggaa actttaccat
     3181 atcataatat cggccgagct tgcaccaaag ctcgaaatca ccgatcatcc tggcgccgga
     3241 aaaacctccc actttagtaa aactgtccag tcttattatt gaagatcccg gggccctgct
     3301 aaaatggcca aatccatcga aattttccag gtaaatggct gcgggtccaa tacatatggg
     3361 aaacggattc tcattgctta caattgaagc aaggccaaaa cctgccgagg gaaaccgttc
     3421 catataatta atcattacct ccaaaccgta ataataaatt aagtcgtctg catctaaata
     3481 tttgatgtac ttccctcggg catatgacgc tgccttattc ctgttcggat agtcgccaag
     3541 attcttttca ttgacataga ctttaacccg ctcgtctttg gcggcgtagc ctttcgcaat
     3601 ctccaccgtc ttatctcctg agcagtcgtc tacgatgatc aattcaaaat tccggtagga
     3661 agaaaccaat acgctttcaa tagcttgcgc gatatacttc tccctgttgt aggacgtcat
     3721 caaaacacta acaagcggcg tagcactcat attgtccaaa tcctggtcaa ccccgggtcg
     3781 ctgaccttaa atagatgtca ccaaaacccg tctcactaaa tttcctgctg tttaccaatc
     3841 gtaacatgtc ttcgaccttg atgaattccg gcatttcaaa ctccatactt tcacccaccg
     3901 aataattaca gtcgatatcc gggttcaccg acctgaccct ttcgatacag gcaatggcac
     3961 gctcggtctg ttccggcgtt gtgtattcaa aggaaatcat tttgaccggc gcgttcagcc
     4021 ctttaagcac ttccaattca tagccctcca cgtctatctt tataaaggcc ggggttccgt
     4081 acgtagcgat cagtttgtcc agggtcgtca tctgcacctt tccggcattg gtccattcgt
     4141 tttccttaaa ccggccgttc tttacagaat ctatccagtt ctttgaaaag gaggaaagat
     4201 aagaagcgtc ggatatatag aactccctga cttcttcggt ttcgcccagg cccatgggaa
     4261 ccaccgctat ctttttgccg aatcgccaat gcaggacttt ctggcagaac gcctgaggct
     4321 ccacggctac gatccttgcc ttggctgcca gcaggggccc cactctattg cccagattgg
     4381 ctcccacatc aaaacacaga tcaccggcct taacaaactg cgaataaaat gcctgtcgtc
     4441 tcaccatatc cgcttgttcc ttccggtaaa attcgctggg caggatcctg ttcttccatt
     4501 cccgcatccg atagacgttg aggtcgccca gcaaggactt agctgcctgc tttatttttg
     4561 ccatgaatat ttaactttaa agagttatcg cccttcacga agatagcgtc ggcccatagg
     4621 ttctttccgt taaggcgcag gtcccggtgt atattgtaca gaccaaaggg caaatagtca
     4681 aattgcctta agtatagggc aatgtcttcg tagaacggct gatcttcata cgctttaata
     4741 aaccaaacct ccgtataaat aaggctgatc gcgccgcgtt tcaacagttc ctcgccgccc
     4801 ttcaatatat tcagctcgtt cccctgggtg tccattttca gaatatcgat atggacgata
     4861 ttcctggatt tggcgtagtc gtcaagtttt tccagtcgca cctcgtatct ttccgccagg
     4921 ccggtatgat cgtcaatgaa tgaacctgtc ctgaccggcg ccaacaagga actcgccgca
     4981 tagccgctcg tcttataaaa gatatccgtt ccctgaatag cggacaaggc tacgttgctg
     5041 caatagatct ctttcatggc ggaaacggtg gcccgcagct tttggaacga gtcgggctgc
     5101 ggttcaaacg catgaatggc tgccgcgggg taaaggctct tgtacttagt caccgtgcta
     5161 ccttcgctcg ctcctgcatc gaagatgacc ggagccgctt tcccgatgat ccttttttga
     5221 acctcgaagg catccagata ccataggttt ttcttaagcg tgacttccat tccagcctta
     5281 ttcagcaaac cctttatcag tctttttatc attctcgggt tgaattttga gcgtgattgc
     5341 cggagcattc gacacttcaa tcaccgtttc agaaaaggaa ctgtaataac tgcaatataa
     5401 cttactgcac ctcgtcagca gcgtccagtc cactaccgca tcccttatgg cctctacgga
     5461 atcccggcca aatttcttgg ggtgggccag gatcctgtcg gggccgaaca ggccggtgaa
     5521 atgggattgc gtcctgtagt catccgtcgc gaggaagaaa acggccgagg gatctctgca
     5581 aatttcctcc ctgatagcgt tttcaaataa gaaatcggga ctgttttcaa ccgaggcaag
     5641 attatccgtt ctcctgatat gcaagccgat gcaatggcgt tgcttcatct tttcttcgag
     5701 gcctctgatc ctgtcggcga tagcgggaat gggattcacc caggaaaacc tgtaatggta
     5761 gggataaaaa ttatggcagg tatcaatata ggtcgttttt ccctccgaca g
//
LOCUS       DATGKE010000225         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_570853, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000225 DATGKE010000000
VERSION     DATGKE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570853"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VKU83_03920"
     CDS             <1..492
                     /locus_tag="VKU83_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HLI92724.1"
                     /translation="SYPIRESDGTAEVAPLAPVVQPRVRVVNVTQASGQVPLTEAELV
                     VSGGRGLKGPENWGMVEALARALGAATACSRPVADSHWRPHNEHVGQTGIAIAPNLYI
                     AIGISGAIQHLAGVNRSKVIVVVNKDPEAPFFKAADYGIVGDAFEVVPKMTEAVKKLK
                     GIA"
     gene            586..1188
                     /locus_tag="VKU83_03925"
     CDS             586..1188
                     /locus_tag="VKU83_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HLI92725.1"
                     /translation="MKKIELEIVALSHSITQTHSYAVVLGEVNGLRRLPIVIGGFEAQ
                     AIAVALEKMHPSRPLTHDLMKNFMSAFTIDLQEIIICDLQEGIFYSKLVCVSEHDTVE
                     IDSRTSDALALAVRFGCPIYTFEHILDSAGILMEDSSAKKKKAGVTATVSTTETGTRE
                     NLSSLSLDELNTLLNEVLEQEDYIRAIAIRDEINSRRRSR"
     gene            1276..2094
                     /gene="ispE"
                     /locus_tag="VKU83_03930"
     CDS             1276..2094
                     /gene="ispE"
                     /locus_tag="VKU83_03930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLI92726.1"
                     /translation="MVIFPNCKINLGLRIIRRRDDGYHDLETVFYPLPLCDILEAVQA
                     PDLEYNFSGRHIPGDPLANLAVRAYQLLKKDIQDLPFIQIHLHKKIPIGGGLGGGSAD
                     GAFMLSLLNAQFRLGLDAGRLMEYALRLGSDCPFFLINRPCLGQGRGEVLEPLPLDLS
                     AYSFLLVHPGIHISTAKAFSRCNPRPGGPGLKTVVSQPPAEWRNTLVNDFESPVFAEH
                     PVLHEIKKELYARGAIYAGMTGSGSCLFGIFEKGTAAGEGWNPGYEVISLMGKN"
     gene            2108..>2652
                     /locus_tag="VKU83_03935"
     CDS             2108..>2652
                     /locus_tag="VKU83_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLI92727.1"
                     /translation="MTTLPSPIPMADLTHQYVELEERYGAHNYHPLPVVLSRGKGVFV
                     WDVEGRRYFDFLSGYSALNQGHCHPRIVAALMEQAARLTLTSRAFYSDQLGEYARYMH
                     RYFGYDKILPMNTGVEAVETAIKLCRRWGYRVKGIPENKARIIACAGNFHGRTGTVIS
                     FSTDPASTRQFGPYAPGFVIIP"
BASE COUNT          577 a          750 c          771 g          554 t
ORIGIN      
        1 tcctatccga tcagggaaag cgacggaaca gcggaagtcg cgccgctcgc tcccgttgtt
       61 cagccccgcg tcagggtcgt caatgttacg caagccagcg ggcaggtgcc cctgaccgaa
      121 gccgagctgg tggtcagcgg cgggcgggga ctgaagggac ccgagaattg gggaatggtc
      181 gaagcccttg ccagggcgtt gggggcggct accgcctgca gtcgtcctgt tgcagactcc
      241 cactggcgtc cgcacaatga gcacgtaggt cagacgggca tagcaattgc gcccaattta
      301 tatatcgcta tcgggatctc cggtgcaatc cagcatttgg cgggcgtgaa ccgaagcaaa
      361 gttatcgttg ttgttaacaa agacccggaa gcgccgtttt ttaaggcggc ggattatggg
      421 atcgtaggcg atgcatttga agtagtgcct aaaatgaccg aggcggtgaa gaagttgaag
      481 gggatcgcgt gaggagggac ctgtgcaggg cccatccagg gccccccaga taatgtccgg
      541 ggggtctgat atttcgcgaa aatcccttag tttcgtgcat taattatgaa gaagatcgaa
      601 ctggaaatag tggctttgtc gcatagcatt acacagaccc attcctatgc ggtggtgctc
      661 ggcgaagtga acgggttgag acggcttccg atcgtcatcg ggggattcga agcccaggca
      721 attgccgtgg cattggagaa gatgcacccc agccgtccgc ttacgcacga tctgatgaaa
      781 aacttcatga gcgcgttcac gatcgacctg caggagatca tcatttgcga cctgcaggaa
      841 gggatatttt attccaagct ggtttgcgtg agcgagcacg atacggtcga aatcgactcg
      901 cgcacttcgg atgccctggc gctcgccgtg cgcttcggtt gccccatcta caccttcgaa
      961 catatcctcg acagcgccgg catcctgatg gaagacagtt ccgctaagaa gaagaaggca
     1021 ggcgtcacgg cgaccgtctc cacgacggaa acagggacgc gggagaacct gagcagttta
     1081 tccctggacg agctgaatac gctgttgaac gaagtgctgg aacaggaaga ctatatccgg
     1141 gccatcgcaa tccgtgacga gatcaatagc cggcggagat cccggtaaaa ggaatcaaat
     1201 cccggcaacg ggaatcaaat cccggtaacc ggatcaggcc cggtccccgg gcaataccgc
     1261 cgccggcatc catatatggt tatttttccc aactgcaaga tcaacctcgg cctgagaatc
     1321 attcgccgtc gcgatgacgg gtaccatgac cttgagacgg tattctatcc cctgccgctt
     1381 tgcgatatcc ttgaggccgt acaagcgccg gacctggagt acaacttttc cggccggcac
     1441 atcccaggcg accctttggc taacctggcg gtccgcgcct atcagctcct caaaaaggac
     1501 atccaggacc tgcctttcat tcaaatccat ttgcacaaga agatccccat tggcggcggc
     1561 ctgggtggcg ggtctgccga cggcgccttt atgctaagct tgctgaacgc ccagtttcgc
     1621 cttggactcg acgcgggccg gctcatggaa tacgccttgc ggctgggcag tgactgccct
     1681 ttcttcctca tcaaccggcc ttgtctgggt cagggacgcg gcgaggtgct ggaacccctt
     1741 ccgctcgatc tctcagctta ttccttttta ttggtgcatc cggggatcca tatcagtacg
     1801 gccaaggctt tttcgcgctg caacccgcgg cccggcggcc cggggttaaa aacggtggtc
     1861 tcccagccgc cggcagaatg gcgaaacacc ctcgttaacg attttgaatc gccggtcttc
     1921 gcggaacatc ccgttcttca tgagatcaag aaggagctgt atgcgcgtgg cgcgatctat
     1981 gccgggatga ccggaagcgg ttcctgcctg ttcggcatct tcgaaaaggg gacagcagcc
     2041 ggcgaagggt ggaaccccgg ctatgaggtc atttccctga tgggaaaaaa ttaattacct
     2101 tgacaagatg actacacttc ccagtcccat acctatggct gacctgaccc atcaatacgt
     2161 ggaactcgaa gaacgctacg gagcgcacaa ctaccacccc cttcccgtgg tcctgagccg
     2221 cggaaaaggc gtattcgtgt gggatgtaga aggccggaga tatttcgatt tcctgagcgg
     2281 ctattcggcg ttgaaccagg gccattgcca tccgcgcatc gttgctgccc tgatggaaca
     2341 ggcggcgcgc ctgacgctga catcccgggc cttttacagc gaccagttgg gcgagtatgc
     2401 ccgctatatg caccgctatt tcggttatga taagatcctg ccgatgaaca cgggggtcga
     2461 ggcggtggaa accgcaataa aactatgccg ccggtggggt taccgggtaa aaggcatccc
     2521 ggagaacaag gcgcggatca tcgcctgcgc gggaaatttt catggccgta cgggcaccgt
     2581 catttccttc agcaccgatc cggcgtccac ccggcagttc ggtccctatg cccccggctt
     2641 tgtcatcatt cc
//
LOCUS       DATGKE010000226         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_956760, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000226 DATGKE010000000
VERSION     DATGKE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956760"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VKU83_03940"
     CDS             complement(<1..1306)
                     /locus_tag="VKU83_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI92728.1"
                     /translation="MRLKSLEIKGFKSFADKTHISFDEGITGIIGPNGCGKSNIIDSI
                     RWVIGEQKISHLRSENLESLVFNGSRTRSASGLAEVSLTFENTKNLLPTEFSTVTVTR
                     KFYKSGESEYRLNDVSCRLKDIQNLFMDTGISTDSYAIIELGMVDDIIKDKDNSRRRM
                     LEQAAGVTIYKTRKKEARQKLDATEQDLARIEDLLFEINNQLKSLENQAKKAEKYFEI
                     KKEYRELSVELAKAALEGFNLTYRELNAQTEAETDRKLRLEAVIATEEAGVEKEKLGF
                     IEKERALQSMQHEFNELLEVLRSKENDKNLASQRLNFLREREESLQEFLAKGEGQLTG
                     LEESIVFTRQQAEEEQNKLSGLQEQLELLKETVDETRRVFDDKRVAVDALRRENAQIQ
                     REQFEAEKKVAVADTSIQNLQRGIRQLEEERTVRASQLRQLQE"
     gene            1516..2352
                     /locus_tag="VKU83_03945"
     CDS             1516..2352
                     /locus_tag="VKU83_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI92729.1"
                     /translation="MNNFIVPIDFSDASKNAARYAAHISNGLADAQLILYNVFEALEY
                     GSDSSPLSTEGGEEEARKSIVELALESVRTDIAALTNARISIVAEESNHFLDSLEAYV
                     LNNDIQFVVMGTTGSTRLGEMLMGGNTLSIVKRRIVPVIIVPPAASSTSAKNVMLLTD
                     FKDIDFTIPIHTVKAVLDLFKPRLHVVNVDHEHYVQVTEEYETQRRKLEASLSDYNPE
                     FYFIRLFDFLDAINQFVADNRIDLILTFPRRHSFLGNVFKTTSTVKLAYHSHVPLVAI
                     TG"
     gene            2506..>2806
                     /locus_tag="VKU83_03950"
     CDS             2506..>2806
                     /locus_tag="VKU83_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92730.1"
                     /translation="MKSAYITIAIAGLLALPGFGQVKTQQVDLSSQISKQSIDELPFR
                     KGNWEFPLMQNFGYSHQSISEQGQSYGHQENFKLDLGAKYFFSDHLGVGLNLNMRS"
BASE COUNT          602 a          814 c          639 g          751 t
ORIGIN      
        1 cttcttgcag ctgcctgagc tgactcgctc tcaccgtccg ctcttcctcc agttgccgga
       61 tgccccgctg tagattttga atggaggtgt cggcaaccgc cacttttttc tccgcttcaa
      121 actgttcgcg ctggatctgc gcattctccc ggcgtaaggc gtcgactgcc acgcgcttgt
      181 cgtcgaacac ccgccgggtc tcgtcgactg tttctttcag cagctccagc tgctcctgca
      241 aaccggaaag cttattttgc tcttcctccg cctgttgcct ggtaaagacg atgctttcct
      301 caagcccggt cagctgtcct tcgcccttgg ccagaaattc ctgcaggctt tcttctcttt
      361 cgcgcaggaa gttcaaccgc tgggaagcga ggtttttgtc attctccttc gaccgcaaaa
      421 cctccaacag ttcattgaat tcatgttgca tcgattgcag cgcccgctcc ttttcgatga
      481 agcccagctt ttccttctcg acgccggctt cttcggtggc gataactgcc tccaggcgca
      541 gtttgcgatc cgtctccgcc tccgtttggg cgtttaactc ccggtaggtg aggttgaacc
      601 cctccaacgc cgctttggcc aactcgacgc tcaattcacg gtattccttc tttatttcaa
      661 agtacttctc cgccttcttc gcctggtttt cgagactctt caactggttg tttatttcga
      721 acaacaggtc ctcgatccgc gccaggtcct gctccgtggc gtccagcttc tgccgggctt
      781 ccttcttcct ggttttatag atcgtgacgc ccgcggcctg ctccagcatc cggcgccggc
      841 tattatcctt gtccttgatg atatcgtcca ccattcctaa ctcgatgatc gcgtagctgt
      901 cggtactgat ccccgtgtcc atgaaaaggt tctggatgtc tttcagccgg caggaaacgt
      961 cgttcaaccg gtactcgctt tccccgctct tgtagaattt gcgggtcacg gtcaccgtgc
     1021 tgaactctgt cggcagcaaa ttcttcgtat tctcaaatgt caggctcact tcagccagtc
     1081 ccgacgcgga tcgcgtcctg ctgccgttga acacgaggct ttccaggttc tccgatcgca
     1141 aatgactgat cttctgttcc ccgatcaccc accggatgct gtcgatgatg ttactctttc
     1201 cgcatccgtt cgggccgatg attccggtaa ttccttcgtc gaaactgata tgggtcttat
     1261 ccgcaaaact cttgaacccc tttatttcca gactctttaa tcgcacgata aatttggttt
     1321 attaggtcac aaaatcaacc tccacgctgt atgccgctcc tgtggccggc cggcaaacct
     1381 acagtaaaat agctaatctt cccgaatatc ggccgctgca gttaacggcc tgttgaaaac
     1441 aggtgaataa caatattgcg gacattttta tatatttacc tgcctgtaag caccaaacag
     1501 aacaaaacga taattatgaa caatttcatt gtgccaattg acttttcgga cgcctcgaag
     1561 aatgcagccc ggtatgccgc ccatatttca aatgggcttg ccgacgcgca gctgatcctt
     1621 tacaacgtgt ttgaagcgct ggaatacgga tcggacagca gcccgctgag tacggagggc
     1681 ggagaggaag aagcccggaa gtcgatcgtg gaacttgccc tcgaaagtgt ccgaacggat
     1741 atcgctgcac tcaccaatgc ccgcatttct atagtggccg aggagagcaa ccatttcctg
     1801 gattccctgg aagcctatgt cctgaataat gacatccaat ttgtcgtcat ggggacgaca
     1861 ggctcgacgc ggcttggcga aatgctgatg ggcggcaata cgctcagtat cgtaaagagg
     1921 cgcattgtcc cggtaatcat cgttccgccg gccgccagtt caacgtcggc caagaacgtc
     1981 atgctgctta ccgatttcaa ggatattgat tttacgatac ctattcatac cgtgaaggcg
     2041 gtgctggacc tttttaaacc gcggctgcac gtcgtgaacg tggatcatga acattatgtg
     2101 caggtgacag aggaatacga gacgcaacga cgcaagctcg aagcgagcct gtccgattac
     2161 aaccctgagt tctatttcat ccgcctcttc gactttctgg atgcgatcaa ccagttcgtg
     2221 gcggataacc ggatcgacct cattctgacc tttccgcgcc ggcattcttt cctcggaaat
     2281 gttttcaaga ccaccagcac ggtaaagctg gcctatcaca gccacgtgcc gcttgttgcc
     2341 ataacaggat agccggccac cttttaaaat tcactggttt ccaaccgatt cggcaaattc
     2401 gaggcatccc cgcccgaatg ccggcaagtc ttttttgtcc atttcaaaac attttgtatt
     2461 ttttatatat gaaaattcac cctcaacccc taaacactgc tgtttatgaa aagcgcttac
     2521 attacgatcg ccatcgccgg tcttcttgcg ctgccgggct tcggccaggt taaaacgcaa
     2581 caagtcgatc tcagctccca gatctcgaaa cagagcatcg atgaactccc attcaggaaa
     2641 ggaaactggg aattcccact gatgcagaat tttggctatt cgcaccaatc catctctgag
     2701 cagggccaga gttacggtca tcaggaaaat ttcaagctcg acctcggcgc aaaatatttt
     2761 ttttccgacc acctgggagt cggtcttaat ctcaacatgc gatcaa
//
LOCUS       DATGKE010000227         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_868524, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000227 DATGKE010000000
VERSION     DATGKE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868524"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VKU83_03955"
     CDS             <1..510
                     /locus_tag="VKU83_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92731.1"
                     /translation="IVELLDDNDQPVAAGQPGEVTITTLGIEAMPLLRYKTGDICTWS
                     DTPCSCGRKTLRLSPIIGRRQQMIKFKGTTLYPPALFDILVEMEQVLDFVVEVYSNEV
                     GLDEIVLHIVRADTGEETDHRIRAYLQARLRVSPHINYLTQDQLQKKQFPEAARKAIK
                     FIDRRHDHK"
     gene            521..2065
                     /locus_tag="VKU83_03960"
     CDS             521..2065
                     /locus_tag="VKU83_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HLI92732.1"
                     /translation="MTPVGQQPLTLDDFAGLLYHRSKLTLDKAALEKVKANFDFLTGF
                     SADKLIYGINTGFGPMAQYKVEKEYLLQLQYNLIRSHSSGSGSLLPPVLCRAAVIARL
                     NSMMQAHSGVHPELCELLRALIDLDIAPCIYEHGGVGASGDLVQLAHLGLTLIGEGEV
                     WFEERVHPTSEIFAKFGVKPLSIYIREGLAILNGTSVMTAIGLVNILRARKLVQWSAV
                     LSAMTNEVVGAYDDHYSRELNQVKQHKGQHRIAAMLRDLLKDSKLIRNRPEHLYHSGS
                     LEQEIFEDKVQEYYSLRCVSQILGPVHDTVCQAEKVLVDELNSVSDNPIVDHLNHNIF
                     HGGNFHGDYVSLEMDKLKIAITRLSMLSERQLNYLLNDRLNQKFPPFLNLGILGVNFG
                     MQGVQFTATSTVAENQTLSFPMYVHSIPNNNDNQDIVSMGSNAALLAKKVIDNSFEVL
                     VIQLMALAQAVDFLECRDRLAPLTAIVYKKIREIFPRFVEDQPRYKDMAALRIMLEQA
                     EPVIGF"
     gene            2092..2820
                     /gene="fabG"
                     /locus_tag="VKU83_03965"
     CDS             2092..2820
                     /gene="fabG"
                     /locus_tag="VKU83_03965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HLI92733.1"
                     /translation="MKCALVTGGSRGIGRAVCARLAGDGYHILINYLSNRDEADKTLD
                     LIRRQGGDGELLPFDVADAAQVKSVLGGFIDAHPDHIIEVLVNNAGIKDDALMVWMND
                     DQWSSVIRTSLDAFFQVTRLVLNGMLLHKYGRIINVVSLSGLKGVAGQTNYSAAKGGV
                     IAATKALAQETARHGITVNAVAPGFIRTDMTGDLDEKEMRMVVPVRRFGTPEEVAHAV
                     AFLASPGASYITAEILSVNGGLYS"
BASE COUNT          653 a          840 c          771 g          591 t
ORIGIN      
        1 atcgtggagc tcctggacga taacgaccag cccgtcgcgg caggccagcc gggcgaggtg
       61 accatcacta ccctgggcat cgaagcgatg ccgctgctcc ggtataagac cggggacatc
      121 tgtacctggt ccgatacgcc ctgctcctgc ggtcgtaaga ccctgcggct gtcgcctatt
      181 atcggccgcc gccagcaaat gatcaagttc aaagggacaa cgctgtaccc gcccgccctt
      241 ttcgatatcc tcgttgaaat ggagcaggtg ctggattttg tggttgaggt ttactccaac
      301 gaagttggtc tggacgagat cgtcctgcac atcgtgcggg cggataccgg cgaagaaacc
      361 gaccaccgga tcagggccta tctgcaggca agactgcggg taagccccca tatcaattac
      421 ctcacgcagg atcagttaca gaaaaaacaa tttcccgaag ccgccagaaa agcgatcaaa
      481 ttcatcgacc gtagacatga tcacaaataa aatttcacgt atgacacccg tcggacagca
      541 accgctaacc ctggacgatt ttgccggtct gctctatcac cggtcaaagc ttaccctgga
      601 caaggctgcc ttggaaaaag tgaaggccaa tttcgatttc ctcaccggct tctcggcaga
      661 taagctcatc tacgggatca ataccggttt cggtccgatg gcgcaataca aggtagaaaa
      721 ggaatatctt ctgcagctcc aatacaacct catccgtagc catagctccg gcagcggcag
      781 cctgctgcct cccgtgctct gcagggccgc cgtgatcgcc cggctcaata gcatgatgca
      841 ggcgcattcg ggggtacatc ccgagctttg cgagctgctc agagccctga tcgatctcga
      901 tatcgctccc tgcatctacg agcacggcgg cgtcggcgcc agcggcgacc tggtacagct
      961 cgcccatctg ggtcttaccc tgatcggtga aggggaggtc tggttcgagg agcgcgtcca
     1021 tccgacaagc gagatcttcg caaaattcgg cgtgaagccc ctatcgatct atatccgcga
     1081 aggactggct atcctgaacg gcaccagtgt catgacggct ataggtctgg tcaacattct
     1141 ccgggccaga aagctggtgc agtggtccgc ggtactttcg gccatgacca acgaggtcgt
     1201 cggcgcttat gacgatcatt attcccgcga gctcaaccag gtaaaacaac ataaaggaca
     1261 gcaccgtatc gccgcgatgc tccgcgacct ccttaaggat agcaagctca tccgcaaccg
     1321 gcccgaacat ctttatcact ccggaagcct ggaacaggag atcttcgagg ataaggtgca
     1381 ggaatattat tcgctgcgct gcgtctccca gatcctgggc cccgtgcatg atacggtttg
     1441 ccaggccgaa aaggtactgg tcgacgagct gaattccgtg agcgacaatc cgatcgtcga
     1501 tcatctcaac cacaatatct ttcacggtgg caatttccac ggcgattatg tctccctgga
     1561 aatggataag ctcaagatcg ccatcacacg gctctccatg ctctcggaac gacagctcaa
     1621 ttacctgctg aacgaccggc tcaaccagaa attcccgcct ttcctgaatc tgggcatcct
     1681 gggtgtgaat tttggcatgc agggcgtgca gtttacggcc acttcgaccg ttgccgagaa
     1741 tcagacccta tcctttccca tgtatgtgca cagcattccg aacaacaacg ataaccagga
     1801 tatcgtgagc atgggcagca atgccgcgct gctggccaaa aaggtcatcg acaactcctt
     1861 tgaggtcctg gtcatccagc tcatggcgct cgcccaggcc gtcgatttcc tggaatgccg
     1921 cgaccggctc gccccgctga ccgccattgt ctacaagaag atcagggaaa tattcccgag
     1981 atttgtcgag gaccagcccc ggtataagga catggccgcg cttaggatca tgctggaaca
     2041 ggcggaaccg gtcatcggtt tttgaaggtc atattaacta atctacagca aatgaagtgc
     2101 gcactagtga ccggtggatc gagagggata ggaagagccg tttgtgcaag gcttgcaggg
     2161 gacggctatc atatcctcat caactacctg tcgaatcggg acgaggccga taagaccctg
     2221 gacctgatcc gccggcaggg cggggacgga gaattgctgc ccttcgacgt tgcagacgct
     2281 gcgcaggtga agtccgtact gggcggtttc atcgatgctc atcccgatca catcatcgag
     2341 gtgctcgtca ataacgccgg catcaaggat gacgccctga tggtctggat gaacgacgat
     2401 caatggtcaa gcgtgatcag gaccagcctg gatgcttttt ttcaggtgac ccgtctggtg
     2461 ctgaacggca tgctcctcca caaatatggc cgtatcatca atgtcgtctc gctctccggg
     2521 ctcaaaggcg tcgcaggcca gaccaactat tccgccgcca agggtggggt gatcgccgcc
     2581 accaaggcgc tggcgcagga gacagcccgg cacgggatca cggtgaatgc cgtcgcgccc
     2641 ggctttatcc ggaccgatat gaccggcgac ctggatgaaa aagagatgcg gatggtagtg
     2701 cccgtccgcc gattcggcac accggaagaa gtagcgcacg ccgtcgcctt tttggcatcc
     2761 cccggcgcct cctatatcac ggctgaaata ctttcggtga atggcggact ctactcctga
     2821 tataacctcg tacctccctc agcggccccc cttca
//
LOCUS       DATGKE010000228         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_754624, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000228 DATGKE010000000
VERSION     DATGKE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754624"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VKU83_03970"
     CDS             complement(<1..1248)
                     /locus_tag="VKU83_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92734.1"
                     /translation="MLTFNRRIAAHFSLNFLNLKLTPNTVLDILSHWRTFFYRSNTFR
                     SYGDEEPLRSELYSLDQLVRHGAVLAAAHKLLPRKCSDQLLKRLDDNEHILKEVRTML
                     VESLASERTVTPAAEWLLDNFYLIEEQILIARTHLPKGYSEGLPCLADGNSAGLPRVY
                     DIALELISHSDGRVDEDSLNSFVAAYQTTTLLTLGELWAIPIMLRLAVIENLRRVAGK
                     IALDRIDDNLAGYWAEKMTTVVRESPANLILTIADMARSKPVLDSPFVAAFTRKLQGK
                     GPALALALTWMEQQLAAAGTSSTELVRQENQKQAADQVSIRNSIGTLRVISATDWRDF
                     VEPLSAVEQVLRQDPAGAYPRMNFATRDRYRHVVESIAKNSALSEKEVAMKALELSAG
                     GGQPGRQGHLGYFLIDKGRRQTER"
     gene            1383..1631
                     /locus_tag="VKU83_03975"
     CDS             1383..1631
                     /locus_tag="VKU83_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92735.1"
                     /translation="MRLSEFVALNENEKRSTVLHQGVLLAKRSSFDSMVFLFQLGSYY
                     VEAYCNPANKAIEEYLIFSNTGVLQPYLEAIPLDNLLN"
     gene            1835..2164
                     /locus_tag="VKU83_03980"
     CDS             1835..2164
                     /locus_tag="VKU83_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI92736.1"
                     /translation="MNIYVSNLSFNVQDEDLREFFTPYGEVTSAKVIMDKITNQSRGF
                     GFIEMPDEAASRKAIAELDGATVENRTIKVNEARPKEQRPARFDNSRNDGGRNYNNAN
                     SYNKNRY"
     gene            2431..2712
                     /locus_tag="VKU83_03985"
     CDS             2431..2712
                     /locus_tag="VKU83_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI92737.1"
                     /translation="MNMYVSNLGYQVTDEELKSMFSKYGEVTSAKVIADRETGKSRGF
                     GFVEMADEAAEKAMKELDGTNADGRTMSVSKAKPKPDGGSSQRTRSNRW"
     gene            2822..>3480
                     /locus_tag="VKU83_03990"
     CDS             2822..>3480
                     /locus_tag="VKU83_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92738.1"
                     /translation="MTTGSFIDNLLYTPSYGWKDQSDALVVPTSRQLWAETFSRINIF
                     KSKKNWISFISVLMLVGLLPFFFLFLFRYFSWYLVGVIVMYSMVIMGTHGTIWLHRYC
                     THKSFGFSRPIWRIITQNLVIKTVPEEIYVVSHHVHHCKSDEPGDPYNAQAGFWYCML
                     AEYNHQRVSPDLGAEAHKKALHFMDHTGVKLNSYKQYQKWGSLASPGYTIGVWVLNWA
                     F"
BASE COUNT          801 a          880 c          909 g          890 t
ORIGIN      
        1 ccgttccgtc tgccggcggc ctttgtcgat aagaaagtac ccgagatgtc cctgtcttcc
       61 cggttgcccg ccgccggccg acaactccag ggctttcatg gccacttcct tttccgacag
      121 cgcggagttc ttggcaatgg actccacgac gtgccggtac cggtcgcggg tggcgaaatt
      181 catccgcggg taggcgccgg cgggatcctg gcgcagcacc tgctccacgg cgctgagtgg
      241 ctccacgaaa tcgcgccagt ccgtcgcgct gatgacgcga agggtaccaa tgctgttgcg
      301 gatcgagacc tggtcggcgg cctgtttttg gttctcctgc cgcacgagtt ccgtactgct
      361 ggttcctgcc gcggccagct gctgttccat ccaggtaagg gccaaggcaa gcgccgggcc
      421 ttttccctgc agcttccggg tgaaggcggc tacgaacggg ctgtccagga ccggtttgga
      481 ccgcgccatg tcggcaatgg tcaggatcag gttggcgggg ctttcccgca ccacggtcgt
      541 cattttctct gcccaatagc cggcgaggtt gtcgtcgatc cggtccaggg cgattttccc
      601 ggcgacccgg cgcaggttct cgatcacggc caggcgcagc atgatcggga tcgcccacag
      661 ttcgccaagg gtcagcaggg tggtggtttg ataggcggcg acaaaactgt tgagactgtc
      721 ctcgtccacc cggccgtcgc tgtgcgagat gagctccagg gcgatatcgt agacgcgggg
      781 aagtccggcg gaattgccgt ccgccaggca aggcaggcct tcgctgtagc ctttcggcag
      841 gtgggttcgc gcgatgagga tctgctcctc gataaggtaa aaattatcca gcagccattc
      901 ggccgcggga gtaaccgtac gctccgacgc gaggctttcg acgagcatgg tgcgaacctc
      961 cttgagaata tgctcgttgt cgtccagtct ttttaaaagc tgatcactgc atttgcgggg
     1021 cagcaattta tgcgccgcgg ccaataccgc cccatgacgg accagctgat cgaggctgta
     1081 gagttcggat cgcagcggtt cttcgtcccc ataactccgg aaggtattgc tccggtaaaa
     1141 aaacgtgcgc caatgcgaaa ggatgtcgag gaccgtgttg ggtgtaagtt tcagattgag
     1201 aaagttaaga gaaaaatggg cggcaatacg ccggttaaag gtaagcactt caaaaataat
     1261 accgcgggat tcatttaata aggctacctt acaggcttcg ctacttcccc cggttaagat
     1321 tgtttttcag cttaaggttt ttagtcaata atttaaaatg ttcacttttt aaaatgttaa
     1381 ctatgagact ttctgaattt gtcgcattga acgagaacga aaaaagatcg actgtcctcc
     1441 accagggtgt gcttctcgcc aagcggagca gctttgactc gatggtcttc ctgttccaac
     1501 tcggcagcta ttacgtggag gcgtattgta atcccgcgaa taaggcgatc gaagagtact
     1561 tgatcttttc caacaccggc gtgctgcagc cttatttgga ggccattcca ctcgataacc
     1621 tgctgaactg agcggttcgc cgattccatt tttcttatcc cgttcccggc aacgggataa
     1681 tttatttggt aactccagga agaagtggta tctttacacc gttaatttga gttctgcatt
     1741 tgtatttgtt aatttatccc gcctcacgac caggatttct tttctttaca ttgtttgctt
     1801 ttctcatctc tctttcattt tttaatttct aagtatgaac atttatgttt ctaacttgag
     1861 cttcaacgta caagacgaag acttaagaga attttttact ccttatggcg aagtaacttc
     1921 cgcgaaggtc atcatggaca agatcacgaa ccaatcccgg ggattcggtt ttatcgaaat
     1981 gcccgatgaa gcagcttcga ggaaggcgat cgctgaactg gatggcgcga cggtcgaaaa
     2041 ccggaccatc aaggtaaacg aagccaggcc caaagagcaa cggccggctc gcttcgacaa
     2101 cagccgcaat gacggcggcc gcaattacaa caacgcgaac agctacaata agaacaggta
     2161 ttagtatttg tttcgattga ccttaaaagc tccggtttcc cggggctttt tttgttaaaa
     2221 aaccttcccg gtacgatgga gggacgatgg ggcgacagcc agcgcctgac cgggaaataa
     2281 tttggccgct tccgatttta aagcctatct ttagggcatt gagataaaca gcatcaagta
     2341 ttcgattcgc ttctttcttc accacatcgt ttcgtttcct ctacttttta ttgcatgctc
     2401 agtcactttt aatttattaa tttttttatt atgaacatgt acgtatcgaa tctgggctac
     2461 caggtaacag acgaggaatt gaaatcaatg tttagcaaat acggtgaggt aacgtccgcg
     2521 aaggtgattg ccgaccggga aaccggaaaa tcgagaggat ttggattcgt ggaaatggcc
     2581 gacgaggccg ctgaaaaagc gatgaaggaa ctggatggaa cgaacgcgga tggcagaacg
     2641 atgtccgtct ccaaagccaa gcccaaaccc gacggcggct catcccagcg cacgcgcagc
     2701 aatcgttggt aaataataat gtccggaagt aagaagatat caagagcctc tgaaacaaag
     2761 tttcggaggc tcccctgtat tttacccgtg cactgattcc aatattctaa aatgacttca
     2821 tatgactaca ggctcgttta tcgacaatct cctttatact ccttcttatg gctggaaaga
     2881 tcaatcggac gcccttgtcg ttcccacgtc aagacaactc tgggcggaga ccttttcccg
     2941 gatcaatatt ttcaagtcga agaagaactg gatctcgttc ataagcgtgc tgatgctggt
     3001 cgggctgctc cctttctttt ttttgttttt gttccgatat ttttcctggt atttggtcgg
     3061 cgtcatcgtg atgtacagca tggtcatcat gggtacgcat gggaccatct ggcttcaccg
     3121 gtattgcacc cataagtcct ttggctttag ccgcccgata tggcggatca tcacgcagaa
     3181 cctcgtcatc aaaaccgttc cggaagagat ttatgtggta tcgcaccacg ttcatcattg
     3241 caaatcggat gaaccgggag acccgtataa cgcgcaggcc ggattctggt attgcatgct
     3301 ggcggaatac aatcatcagc gggtttcgcc tgacctggga gcggaggcgc acaaaaaggc
     3361 attgcatttc atggatcaca ccggcgttaa attgaattcc tacaaacaat atcagaaatg
     3421 gggctccctg gcctccccgg ggtacaccat cggggtttgg gtgctgaatt gggctttttg
//
LOCUS       DATGKE010000229         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_425390, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000229 DATGKE010000000
VERSION     DATGKE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425390"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKU83_03995"
     CDS             complement(<1..644)
                     /locus_tag="VKU83_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92739.1"
                     /translation="MKQRQRNWGSVGKMLFILCFSLGLAYGASAQRSMGHGYVGGGFH
                     GGYHGYYHPYYAPRVYYVPSFYGDWGYGLGLGFGWGYGLGWGIPGWYGPWYYPAYPPY
                     YYGRGPMPPALNDEIRGIRSDYKAEIKDVKHDKALSHKEKEEKINQLEQERDAAITKA
                     RHDFFEAQLRQRYGTPQQNNSTQPQNNSYKTQPGNSNSSNDADHPEYSDKSSETG"
     gene            958..>2047
                     /locus_tag="VKU83_04000"
     CDS             958..>2047
                     /locus_tag="VKU83_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92740.1"
                     /translation="MLPLPAKTAWLACLLLFHLAEMRPATLLAGTAVCLAAATAFFAH
                     IRIRGLLARTRVLEQQVEHRTRQAEEALHTKNLFLTNMSHEIRTPMNGLLGMASLLSQ
                     TPLSKEQAGYVDNIRSCGDTLLTVINNILDFSAMETGRITLDEKVTDLRMLVSEVLET
                     FTSRAEKAGIELQHELSAEIPSRVITDQGRVRQILLNLVGNAVKFTGQGQVTVRVGRA
                     DAQDLPTKGEQIGVTFDVVDTGCGMEKDQLDQWQQFFCRADPASRPLSSTGLGLILAD
                     RLVHLLNGRIRVASEPGTGTRFRFTVLAKPAPQKSIPTTADSLPRLAEKYPLRILLAE
                     DNPINRQLAVLILDKMGYSCGVAENGKEV"
BASE COUNT          473 a          602 c          508 g          464 t
ORIGIN      
        1 cccgtttcac tgctcttgtc ggaatattca gggtgatctg cgtcattcga ggaattacta
       61 ttaccgggtt gcgtcttata gctattattt tgcggttggg tactgttatt ctgttggggt
      121 gttccatacc tttgcctcag ctgcgcctca aagaaatcgt gccttgcttt cgttatcgcg
      181 gcatcgcgct cctgctccaa ctggttgatc ttttcttcct tttccttatg cgacaaagcc
      241 ttgtcgtgtt tcacatcctt gatctccgcc ttgtagtcgc tcctgatccc gcggatttca
      301 tcgttcaatg caggcggcat cgggccgcgg ccgtaataat acggcggata ggcagggtaa
      361 taccagggac cgtaccagcc gggaattccc caacccagcc catagcccca gccaaatccg
      421 agtcctagac catatcccca gtccccataa aacgagggca catagtacac ccttggagca
      481 taatacgggt gatagtaacc atggtagccg ccgtggaacc cacccccgac gtatccgtga
      541 cccatgctgc gctgggctga tgcgccgtag gccaggccaa gtgaaaagca taggataaac
      601 agcattttac cgacacttcc ccaattcctc tgtcgttgtt tcatcgtttt cataatcaat
      661 aaaggtttta ctgttatcgt ttttcttata tcgtatagac gtgaaaaata ggcaggggtt
      721 aaatcgatat ggcggttata actaatctat aacattacct ttaccagccg ttaacctcac
      781 ggatcgcgga tttgccgtga cgggctcaac cctgccccgc ggtcaagaaa ttagaggaaa
      841 acacttatcg ctaaaaaaat taaggagccc ggcgacaaaa tacaaccgga ttgcaaccgt
      901 ttacagacta accgaaaata ggcaatagtt attcgttaag ctctgaaacc caagctgatg
      961 cttcccttac ccgccaagac cgcctggttg gcatgtctgc tccttttcca cctggccgaa
     1021 atgcggcccg caaccctttt ggccggaaca gccgtctgcc tggcggcggc gacagccttt
     1081 ttcgcccaca tcaggatccg ggggctgctt gcccggaccc gtgtgttgga acagcaagtt
     1141 gaacatcgca cccggcaggc agaagaggcg ctccacacga agaatctttt cctgacaaat
     1201 atgagccacg agatccgcac acccatgaac ggcctcctgg gcatggcctc ccttctttcc
     1261 cagacgcccc tcagtaagga acaagcgggc tatgtagaca atatccgatc ctgcggcgac
     1321 acactgctta cggttattaa caatatcctc gacttctccg cgatggaaac cggcaggatc
     1381 acgctcgatg agaaagttac ggaccttcgc atgctggtat ctgaagtcct ggaaacattc
     1441 accagccggg ctgagaaagc cgggatcgag cttcagcatg aactgtcggc cgaaatcccc
     1501 tcccgcgtca tcacggacca gggccgcgtt cgccagatcc ttctaaacct ggtgggaaat
     1561 gcggtgaagt tcaccgggca aggacaggtt acggtacgcg tcggccgcgc ggatgctcag
     1621 gatctgccga caaaaggaga acagatcggg gtgacctttg acgtcgtcga tacgggttgc
     1681 ggaatggaaa aagatcagct cgaccaatgg cagcagttct tttgccgcgc ggatccggca
     1741 agccgcccgc tgtcgagcac cggcctcggt cttattctcg ccgaccgact ggtccacctc
     1801 ctgaacggcc gcatccgcgt cgcgtccgag cctggtaccg gcacccggtt caggttcacc
     1861 gtactggcaa aacctgcccc gcagaaatcc attccgacaa ccgccgattc actgccacgg
     1921 cttgccgaaa aatatcctct ccgcatcctg cttgccgagg acaatcccat aaaccggcag
     1981 ctcgcggtgc tgatcctgga caagatgggc tattcatgcg gcgttgccga aaatggaaaa
     2041 gaggtcc
//
LOCUS       DATGKE010000230         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_542959, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000230 DATGKE010000000
VERSION     DATGKE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542959"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VKU83_04005"
     CDS             complement(<1..612)
                     /locus_tag="VKU83_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92741.1"
                     /translation="MSRILTFCFLLLLLAHAGRAQFTVGVQVNVLQPVPPYLPQLKAD
                     IIGNRGSQLNQDIGSHLSIVLRYTGQSAQRVKLAGSIERVSPAIMGVSLRPDFQPAQP
                     IVLGPSQPILAVTRDMLQSAFGNFTENSLVYTNCDLNTLRQNGVDFKLPEGTYRVCVT
                     AYDYDRPGFSAPLSAPGTGCAYFTICYTASAPQLILPVSTFVNA"
     gene            complement(621..2330)
                     /locus_tag="VKU83_04010"
     CDS             complement(621..2330)
                     /locus_tag="VKU83_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92742.1"
                     /translation="MRTKLAILSGMLLTVTAIHAQNLDNLKDQKPFTIHGSLGAGAEF
                     YQSNESYPTRPPYAWNVNGSLTPSVYGVSMPLAFVVTQYGKSYSSPFTQLGISPSYKW
                     IKLHLGYRTILFSPLVFAGQSFLGGGIELSPKGFYIGGFYGLLNRAVQEDTSFAHPIE
                     PQYARVGYGIKIGIGSPKNNFSLSFFHAKDDTGSIKRLQDTLTTVLPQENSVVGSSWH
                     FTFFHRLVWTGDLAAGLLNRDQSYQQIDSVGYFKIPTVMRKIMPINWSSVFSYAGQSQ
                     LSFTWKTINASAGYRRIAPDFVSLGVPYMLNDLEMVNGSFGGNFDRGRLNANLAFTTQ
                     HNNLTHLLASTLLTRTGNLALNAFVSRQFNLNTTVTAVQVYQRNGGAALSDSVRMNQL
                     MLSISLAPTLNFFDNNYQHTVSANVSYTNLDDHNPVTAPLTGGNNLSTSLNYALYFSH
                     AFWGLNAAVVYSQYGQGDNRYQSTGINAGMNAQLLSNHQLSLQGSAGYFLNKYSNSPT
                     GNNTTFSFNGDYTLPHHTLGVYLNYVLTPPINLNPLTTIYHIPIAVNSKNFAGGVMYG
                     FHF"
     gene            complement(2344..>3431)
                     /locus_tag="VKU83_04015"
     CDS             complement(2344..>3431)
                     /locus_tag="VKU83_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HLI92743.1"
                     /translation="AFGAIAPVRAQFLKDLTNNLKQTLQSRANGKSNQAANHLLDKVD
                     SAMKIGGAKGTKTGGTGGTGTAATGGAGSLPGMGKGSFDTSGMGRVLGAFVKTAQENP
                     NDTNQADLLNKSLTRMIGGDGVSSQDSAKAIKSFMTAAGGSGVLYESIILTNSKRGTT
                     RDTTYIYLTNSGEGRSEMRLPIPGVKTPKFIVIGRANEPTYSVILDADTRSYSLNVID
                     TALINSGIEKYQVTRIGTESVSGYSCIHSRLVSTTGSGMFATSSTMDLWTSTSVPGYP
                     IYSKLLTFQSSNGGLLGALNKAGAGGFLVKMTAGDGKDFTMTNQLMKAEQRNFPSALF
                     EIPAGYGNEAASTSQRLLSGSVPAKQP"
BASE COUNT          770 a          937 c          916 g          808 t
ORIGIN      
        1 agcattcaca aaggtgctca ccggcaagat cagttggggc gcggaggctg tataacaaat
       61 cgtaaaatac gcacatcccg tacccggcgc cgacaagggg gctgaaaagc ccggccggtc
      121 atagtcatac gcagtgacac aaacccgata ggttccctca ggaagcttaa agtccactcc
      181 gttttgccgc agggtgttga gatcgcaatt cgtgtaaacc agcgaattct ccgtgaagtt
      241 cccaaacgca ctttggagca tatcccgcgt cacggccagg attggctgcg atggccccag
      301 gacgatcggc tgagccggct gaaaatcagg ccgcaacgac acgcccatta tcgcgggtga
      361 tacccgttct atgcttccgg ccaatttgac ccgctgcgcg ctttgcccgg tataccgtag
      421 aacaatcgac aaatgactgc cgatgtcctg gttgagctga ctgccgcgat tgccaatgat
      481 atcggccttt aactgcggca ggtaaggagg cacgggctgg aggacattca cctgtacgcc
      541 aacggtaaac tgcgcccggc ccgcatgggc cagcagcagc aacaaaaagc aaaaggtcaa
      601 tatccggctc ataacatcgt ttaaaagtga aacccgtaca ttaccccgcc ggcgaaattt
      661 ttcgaattca cggcgatggg aatatgataa atggtcgtca gcggattcag attgatgggt
      721 ggcgtcaaaa catagtttaa gtacaccccc agggtatggt gcggcaaagt ataatcgccg
      781 ttaaaggaaa aggtggtatt gttcccggtc ggcgagtttg aatatttgtt tagaaagtat
      841 cccgcgctac cctgtaagga caattgatga ttactcaaca actgtgcatt catgcccgca
      901 ttgatccccg tggactgata gcggttatca ccctgtccat attggctgta cactaccgcc
      961 gcattcaggc cccaaaaagc atggctgaaa tacagcgcat agttcaggga agtgctaagg
     1021 ttgttgccgc cggtcagggg agcggtcacc gggttatggt cgtcaaggtt agtgtaactg
     1081 acatttgcgc tgaccgtatg ctgataatta ttatcgaaga aattgagtgt cggcgcaagg
     1141 cttatcgaca gcatgagctg attcatccgc accgagtcgc ttagcgcagc acccccgttc
     1201 cgctgataga cctgcaccgc cgttaccgtt gtattgagat tgaattgccg ggacacaaat
     1261 gcattgagcg ccaggttccc cgtccgcgtc aggagggtcg aggccagcaa atgcgtgagg
     1321 ttgttatgct gagtggtgaa ggccaggttg gcgtttaacc ggcccctgtc gaaatttccg
     1381 ccgaacgagc cgttgaccat ctcgaggtcg ttcagcatat aggggacacc caatgaaaca
     1441 aagtccggtg cgatccgcct atagcccgcc gatgcgttga tggttttcca cgtgaaggac
     1501 agttgagact ggcctgcata cgagaacacc gaactccagt tgatgggcat gatcttccgc
     1561 ataactgtcg ggatcttgaa atagcccacg ctatcgatct gctggtaaga ttgatcccgg
     1621 ttcagcaacc ccgccgccag atcaccggtc caaaccagcc ggtgaaaaaa agtgaaatgc
     1681 caggagctgc cgaccacgct gttctcttga ggcaggaccg tcgtcagcgt gtcctgcagt
     1741 cgtttgatcg agccggtgtc gtccttggca tggaaaaatg aaaggctgaa attatttttg
     1801 ggacttccga tcccaatctt gattccatac ccgacccgcg cgtattgcgg ctcgatcgga
     1861 tgggcaaagc tcgtatcttc ctgcacagcc ctgttcagga gcccataaaa tccaccgata
     1921 taaaatcctt tcgggcttag ctcgataccg cctcccagga aactttggcc cgcaaatacc
     1981 aacggcgaga acaggatcgt tcgataaccc aggtggagtt tgatccattt ataggaaggg
     2041 ctgatcccca attgggtgaa aggactggaa taacttttcc cgtattgggt gaccacaaat
     2101 gccagcggca tgctcacgcc gtagacagaa ggcgtcagac tcccgtttac attccaggca
     2161 tacggcggcc gcgtaggata gctctcgttg gattggtaga attccgcccc cgcgccaagc
     2221 gagccgtgga tcgtgaaggg tttttggtcc ttcaggttat ccaggttttg tgcgtggatg
     2281 gccgtaaccg tgagcaacat tcccgacagt atggctagtt tggttcgcat acaatcgctt
     2341 tgatcagggt tgttttgcag gtaccgaccc ggaaaggagg cgctgtgagg tcgaggcagc
     2401 ctcgtttccg tatcctgcgg gaatctcaaa tagggcagac gggaaattcc gttgctccgc
     2461 cttcatcagt tgattggtca tcgtgaagtc tttcccatcg cctgccgtca tttttaccag
     2521 gaatccacct gcgccggcct tattgagtgc cccaagcaaa ccgccattgg aagactggaa
     2581 cgtcagcagc ttgctgtaga tgggatatcc aggaacagat gtgcttgtcc acagatccat
     2641 cgtactgctc gttgcgaaca tccccgaccc ggtagtgctg accagacggc tgtggataca
     2701 ggagtacccg cttacgcttt ccgttccgat ccgggtcacc tgatattttt ctatccccga
     2761 attgatcaag gcggtatcga tcacattgag cgaataactt ctggtgtccg catccagtat
     2821 gacggaatag gtcggttcgt ttgcccttcc gatcacgatg aacttcggcg tctttacgcc
     2881 ggggatcggg aggcgcatct ccgaccttcc ctcaccgcta ttggtaaggt agatgtaggt
     2941 ggtatccctt gtggtgcccc gcttggaatt ggttaaaatg atcgattcat ataggactcc
     3001 gcttccgccg gcggcggtca tgaaactttt gatcgccttt gctgaatcct gtgacgagac
     3061 cccatctccg ccgatcatcc gggtgagtga cttgttcagg aggtcagcct gattggtgtc
     3121 attcgggttt tcctgcgcgg tcttgacaaa ggcgcccaat acacgaccca tgccgctggt
     3181 gtcgaaagaa cccttgccca tccccggaag acttcccgct cctccggtcg ccgctgttcc
     3241 cgttcctccg gtcccacctg tcttcgtacc cttcgctcca ccgatcttca tcgcgctatc
     3301 gactttatcc aacaggtgat tcgccgcctg attggacttc ccgttcgccc ggctctgcag
     3361 cgtctgctta agattgttcg taagatcttt caggaattgc gcccttaccg gggcgatcgc
     3421 gccgaaggcg c
//
LOCUS       DATGKE010000231         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_536154, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000231 DATGKE010000000
VERSION     DATGKE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536154"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VKU83_04020"
     CDS             complement(<1..1045)
                     /locus_tag="VKU83_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HLI92744.1"
                     /translation="MKIGILGTRGIPNQHGGFEQFAEFVAPALVQRGHEVWVYNSSLH
                     PMGDTTWQGVHLIKARDPENTMGTSGQFIYDLNCILDARKRKFDVILQLGYTSSSVWT
                     RLLPKDAVIITNMDGLEWKRSKYSPSIQSFLKRAEKWAVDGSDLLIADSKGIQAYLAD
                     KYQKDSKFIAYGATLFTSPDASVLSSYGLPEFGYNMLIARMEPENNIETILKGHLAAE
                     TDDKLILIGNHKNKFGSYLKAQYEDHRTLFLGPIYDLTVLNNLRHFSRIYFHGHSVGG
                     TNPSLLEAMASQALVMAHDNIFNKSVLENDAFYFSCPSDISDLLNRRPKKSDYQLFLQ
                     HNNEKIKESYSWDH"
     gene            complement(1047..2159)
                     /locus_tag="VKU83_04025"
     CDS             complement(1047..2159)
                     /locus_tag="VKU83_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI92745.1"
                     /translation="MKVLFISRATLFTVRGGDTSQIVNTALYLEKLGVQADVRLCDET
                     IDYRGYDLIHFFNVIRPADILKHIERSGKPYVITPIFVDYSEYDRTIRKGMTGFVLRW
                     LSPDGLEYVKAIARLLVNGEKLVSPSYLWLGQRRSIRKILSRAEMILPNSNSELSRLR
                     ARYNGNAPARIIPNAIDPSVFTIDGDTAGRGTGKKESREGDLVISVGRIEGLKNQLNL
                     VRALNGTRYRLILIGSPAPNHKAYYEECRKIAEANISFVDHIEQKELAGYYSRANVHV
                     LPSWFETTGLSSLEAAAMGCNLVITDKGDAREYFEDYAWYCDPASPASIFEAVEKAAA
                     CEFREALREKVFAQYTWPHTAERTLGAYQEIINRSN"
     gene            complement(2156..>2574)
                     /locus_tag="VKU83_04030"
     CDS             complement(2156..>2574)
                     /locus_tag="VKU83_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92746.1"
                     /translation="YLGWHVAAKKSVLFGVGLGQVKELGYELFQHYYKFIFAHDSIAI
                     PNALGDTLATFGVLGLSIRLFVVFWLFFKTRVYSNYYRLSLFLFIFIYQFTGSFIMNI
                     AEYSIWILAFNKGSFEEFNRPAKRRSARLREVSALA"
BASE COUNT          587 a          704 c          654 g          629 t
ORIGIN      
        1 tatgatccca ggaataactc tcctttatct tttcattgtt gtgctgcagg aatagctgat
       61 aatcgctttt cttcggccgc cggttcagca ggtcgctgat gtcggatggg caggagaaat
      121 agaacgcatc gttctccagc acgctcttat tgaagatatt gtcgtgggcc atgacgagcg
      181 cctgagaagc catcgcctct aataatgagg gattggtgcc gcccacagaa tggccatgaa
      241 aatagatccg cgagaaatgc cggagattat ttaacaccgt gagatcatag atcggtccca
      301 ggaatagcgt ccggtggtcc tcgtattgtg ccttcagata cgaaccgaac ttgttcttat
      361 ggttcccgat aaggatcagc ttatcgtccg tttccgctgc cagatgccct ttcagaatgg
      421 tctcgatatt gttctccggc tccatgcggg cgatcagcat attgtaccca aattcaggca
      481 gaccatagga ggaaagaaca ctcgcatcgg gcgaggtgaa cagggtagcg ccgtacgcaa
      541 tgaacttgga atctttttga tacttgtcgg ccaggtaagc ctggatgccc ttcgaatctg
      601 ctatcagcag gtcgctgccg tcgacggccc atttttccgc ccgcttcagg aaagattgaa
      661 tggaagggga atatttgctc cttttccatt cgagaccatc catattggtg atgatcacgg
      721 catcctttgg caatagccgt gtccacacgc tgctgctcgt atagcccagc tgcaggataa
      781 cgtcgaattt tcttttccgg gcatccagaa tgcaattgag gtcataaatg aattgcccgg
      841 acgttcccat cgtgttctcc gggtcccttg ccttgatcag gtgtacaccc tgccaggtcg
      901 tatctcccat aggatgcagc gaagagttgt atacccatac ctcatgcccc ctctggacaa
      961 gggcaggcgc tacaaattcc gcgaattgtt caaatccgcc gtgctgattg ggtattcccc
     1021 tcgtgccgag aatgccgatc ttcatttcaa ttgctcctat tgatgatttc ctggtaagcg
     1081 cccagcgttc gttcggccgt atgcggccac gtatactgtg caaaaacttt ttctctcaaa
     1141 gcctcacgga attcgcaggc cgccgccttt tccacagcct cgaagataga ggccggagag
     1201 gccgggtcgc aataccaggc atagtcctcg aaatattccc tggcatctcc tttgtccgtg
     1261 atcaccaggt tgcagcccat agccgcggcc tccagcgagc tgagccccgt ggtctcgaac
     1321 cagctcggta gaacatgcac attggcccgg ctatagtagc cggccaattc cttttgttcg
     1381 atatggtcca caaaactgat attcgcttcg gcgatctttc ggcactcttc ataataggcc
     1441 ttgtggttgg gggccggaga accgatcagg atgagccggt atcgcgtgcc gtttagcgcg
     1501 cgcaccaggt tcagctggtt cttgaggcct tcgatcctgc ccaccgagat caccagatcg
     1561 ccctctctgc tctctttttt gcccgttcca cggccggccg tgtcgccgtc tatcgtaaaa
     1621 acggatggat ctatggcgtt gggaattatc ctggccggtg cattcccgtt ataacgggcc
     1681 cttaaccggc ttagctcact gttggaattg ggtaggatca tctccgcacg gctcaggatc
     1741 ttccggatcg atcgtcgttg tccgagccaa aggtaagacg ggctgacgag tttttcgcca
     1801 ttgaccagca gccgggcgat ggcctttaca tactccaacc catcgggtga cagccagcgc
     1861 aggacgaacc ctgtcatccc cttgcggatg gtccggtcat attccgaata atccacgaag
     1921 atcggcgtga tcacataggg tttgcccgag cgttcaatgt gcttcaggat gtctgcggga
     1981 cggatgacgt tgaaaaaatg gatgagatcg tatccccggt agtcgatggt ctcatcgcag
     2041 agccggacat ccgcctgtac ccccaatttc tccaggtaaa gggccgtatt gacgatttgg
     2101 gaggtgtctc cccctcttac ggtgaaaagg gtagcccggg atatgaaaag gactttcatg
     2161 cgagggcact gacctcccgc agccgggcag accgtcgctt ggccggccgg ttgaactctt
     2221 cgaaagagcc cttattgaat gcgagtatcc atatgctgta ttcagcgatg ttcataataa
     2281 agcttcctgt gaactgataa ataaaaataa acaggaaaag gctgagccgg taataattcg
     2341 aatagaccct tgtcttaaag aacagccaga acaccacgaa cagccggata gacagcccca
     2401 gcaccccgaa agtagccagt gtatccccca gcgcgttcgg aatggcgatc gaatcatgcg
     2461 cgaaaatgaa cttgtaataa tgttggaaca gctcataacc cagttcctta acctgcccga
     2521 gaccaacccc gaataggaca cttttcttcg ccgccacatg ccatcccaga taga
//
LOCUS       DATGKE010000232         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1010856, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000232 DATGKE010000000
VERSION     DATGKE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010856"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="VKU83_04035"
     CDS             complement(<1..1656)
                     /locus_tag="VKU83_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLI92747.1"
                     /translation="MKWLPLFFLLTSADPFSIVGRRAETFKPLPFGAIRPEGWLLTQV
                     RENLRGLTGHLDSLAPDLILKDDIFGRDRLTKNITRKDVGALAGGNAATEVQYLWWNS
                     ETQGNWRDGWIRSAVLADDPVMLGRAKDYVHRILATQDADGYLGIYDKDLRYHFDGEN
                     GELWSKTTLLRGLLAWYDYTRDPAVLTAIERAVADVMRHYPAGASHPFYSKTPDVGGL
                     SHGLMFTDVLESLYRRTGRVAYLDYALFLYKDFSEATLHEDAQYSKIIDAAYRLRGHG
                     VHTYEHLRAVAAAAYASGNPALQTALDDYLQKIAAEITPSGAPAGDEWIGERQGDATN
                     TGYEYCSLQELMNGYTDLLLKTGLPAYGDKAERLFFNAAQGSRDSGGSCIAYLKTDNS
                     YYMTGGRNGDSSNPRQTRYKYSPVHQDDAVCCAPNAGRIAPYFIARMWMRSSSGLVAT
                     LLGPCEVTTTWKDTNIRIREKTGYPNDYSFDFDVDADHPVRFTLAIRKPAWASGSFIS
                     YPYTEKDGYFLIERTWRPGESLRVRFYPQPQVRRDRDGAAYFAY"
     gene            complement(1691..>2215)
                     /locus_tag="VKU83_04040"
     CDS             complement(1691..>2215)
                     /locus_tag="VKU83_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLI92748.1"
                     /translation="LLEVRGVRLYHDQALYKEPGGGQTPWHADQYYWPLATDRTVTAW
                     IPLQETPLPMGPLEFSARSNQLSTGRDLKISDESQQLLEDALRDKGYPHVIEPFAAGE
                     VSFHNGWLYHRAGANRTDRMRKVMTVIYMDKDMVLKEPENPNQVNDWNSWCPGAKVGE
                     PIRTARNPVLFPKS"
BASE COUNT          415 a          640 c          692 g          468 t
ORIGIN      
        1 gtaagcgaaa tatgccgcgc cgtcgcggtc ccggcggacc tggggttgcg gataaaaacg
       61 aacgcgcagg gattccccag ggcgccacgt cctttcgatc agaaaatacc cgtctttctc
      121 ggtgtaaggg tagctgatga agctgccgct cgcccaggcc ggtttgcgga tggctaacgt
      181 gaagcggacc ggatggtccg cgtcgacatc gaaatcaaag gaataatcgt tcgggtagcc
      241 ggtcttttcc cggatcctga tattcgtgtc cttccacgtt gtcgttacct cacatggacc
      301 gagcagggta gcaaccagtc cggagctgct tcgcatccac atcctggcga tgaaataagg
      361 tgcaatgcgc cccgcgttcg gcgcacagca gacggcgtcg tcctggtgga ccggcgaata
      421 cttgtaccgg gtttgcctgg ggttgctgct gtccccgttc cgcccgccgg tcatatagta
      481 cgaattgtcc gttttgaggt aagcgatgca ggatcccccg gagtcgcggg atccctgggc
      541 ggcattgaag aacagccgtt cggccttgtc gccgtaggcc ggcaaaccgg ttttcagcag
      601 caggtcggtg tagccgttca ttaactcctg cagcgaacaa tattcatatc cggtgttggt
      661 ggcgtcgcct tgcctttcgc cgatccattc gtcgcccgcc ggggcgccgg aaggggtaat
      721 ttcggcggca attttttgca ggtaatcatc gagggcggtt tgcagggccg ggtttcccga
      781 ggcatacgcc gccgcagcca cggcgcggag atgctcgtag gtatgaaccc catggccccg
      841 gagccggtat gccgcgtcga tgatcttgct gtactgtgcg tcttcatgaa gcgtggcctc
      901 cgaaaagtcc ttgtacagga ataaggcata gtcgaggtag gcaacccgcc ccgtccggcg
      961 atacagcgat tccaggacat cggtgaacat cagaccgtgg gaaaggccgc cgacgtcggg
     1021 cgttttggaa tagaaaggat gcgaggcgcc ggccgggtaa tggcgcatga catccgctac
     1081 ggcacgttcg atcgccgtca gcacggccgg atcgcgggta tagtcatacc aggccaacag
     1141 gccgcggaga agcgttgtct ttgaccataa ctcgccgttc tcgccgtcaa aatggtaccg
     1201 caggtctttg tcgtaaatgc cgagataccc gtccgcatcc tgcgtcgcga ggatgcgatg
     1261 cacataatcc ttcgcccggc cgagcatcac cggatcgtcg gccaggacgg ccgaccggat
     1321 ccagccatcg cgccagttgc cctgggtttc gctgttccac caaaggtatt gcacctcggt
     1381 tgccgcgttc ccgccggcaa gcgcgccgac gtctttccgg gtgatgtttt tggtcagacg
     1441 gtcgcggccg aaaatatcgt ctttaaggat caggtccggg gccaggctat cgaggtggcc
     1501 ggtgaggccc cggaggtttt cgcggacctg ggtcaacagc cagccttccg gccggatggc
     1561 gccgaaagga aggggtttga atgtttcggc gcgccgcccg acgatggaaa aagggtcggc
     1621 cgaggtcagc aaaaagaata gcggtaacca cttcatgccg gcagagattt tcggattttc
     1681 ggattttcgg tcaggatttc gggaatagaa caggattacg cgcagtcctg atcggttccc
     1741 cgaccttggc cccggggcac cagctgttcc agtcgttaac ctggttgggg ttctccggct
     1801 ctttcagcac catatccttg tccatataga tcacggtcat caccttccgc atccggtcgg
     1861 tacggttcgc gcccgcgcga tgatacagcc agccgttgtg gaaactgacc tcgcccgcgg
     1921 caaagggttc gatcacgtgc ggatatccct tatcgcgaag cgcatcctcc agaagctgtt
     1981 ggctttcatc gcttattttc agatcccggc cggtagaaag ctggttgctg cgggcgctga
     2041 attcgagcgg ccccatgggc agcggggttt cctggagcgg gatccaggcg gtcacggtcc
     2101 ggtcggtggc aaggggccag taatactggt ctgcatgcca gggcgtctgg ccgccgcccg
     2161 gttccttgta taaagcctgg tcatgataga gcctgacgcc gcgcacctcg agaag
//
LOCUS       DATGKE010000233         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1182589, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000233 DATGKE010000000
VERSION     DATGKE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182589"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VKU83_04045"
     CDS             <1..383
                     /locus_tag="VKU83_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLI92749.1"
                     /translation="EYAISKVALLPMGEGDFIMPLKAAVRKAIRKQKGAKLIVELELD
                     KAVIRPPKELMDCLADEPAALAHFNSLPRSHQNYFGNWVQQAKTAGTRAKRIACVVTA
                     MSRKLNFAEMLRSRSEGRDLLFPK"
     gene            complement(393..1340)
                     /locus_tag="VKU83_04050"
     CDS             complement(393..1340)
                     /locus_tag="VKU83_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLI92750.1"
                     /translation="MSTIHTILGANGIVADSLARELAGRKLAVRLVSRSPAAIPGTST
                     FPADCTDPAQTLEAIRGSSVVYCCIGLPYRYAIWRQQWPRIVDNLIGACRQTGAKLLF
                     FDNVYMYGQTSGPMTEETPYNPSSRKGDLRARLATQLMSEVRKGNITAAIARSADFYG
                     PAADKTSIPNLLVFQRLAKGQKAQWLVNAHARHSFTYVPDAAKALAILAMDESAWNQV
                     WHLPTASQPLTGAEFIDRAAASLHRAGRYTVLQKWMVRLGGLFDRTTAELYEMLYQYE
                     FDYLFDSTKFETAYSFQPTSYEEGIRTIGSQSIATGPGR"
     gene            1499..>2673
                     /locus_tag="VKU83_04055"
     CDS             1499..>2673
                     /locus_tag="VKU83_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI92751.1"
                     /translation="MSINKAWVTGLIVAGCLTGMRARAQKASDTLPEVATVDACVQFA
                     LKHYPLVQQAMLDEQITERQIRSKLADWYPQLGLNAYYQNNFQLPALLFKGAIVYSGT
                     YNQSQVALGLTQTLFNRDALLASQTKRDVMTQIHQYTIGDKIDVAVNVSKAFYDVLLT
                     EQDIKVLDDDIVRLERNLKDTYNQYQGGLVDKTDYKRAIISLNNSKAQRKTDEETLKA
                     KYAALLQLMGYTGRDSLHLVYDSAAMEQEVALDTNAVVDYGQRIEYQELMTQKRLQLA
                     DVKYERWSYLPSVSAGAGYSLNYLNPEFSKLYSDNFPQSYAGLTVSVPIFQGTKRTQN
                     IRIAELQLERIDWDVINLQNQINTQYTQALATYESNLNNYHVQRDNLGLAREVFNTI"
BASE COUNT          644 a          713 c          757 g          559 t
ORIGIN      
        1 atgaatacgc catttcaaaa gtggcgctac tgccgatggg ggaaggagat ttcatcatgc
       61 cgctgaaggc tgccgtccga aaagcgatcc ggaagcagaa aggagcaaag ctcatcgtcg
      121 agttggagct cgacaaggcc gtgatccggc cgccaaagga attgatggat tgccttgccg
      181 atgaaccggc cgccctggcc cacttcaata gtctgccccg ctcgcatcag aattatttcg
      241 gcaattgggt acaacaggcg aaaactgccg gtacccgcgc caagcggatc gcctgcgtgg
      301 ttacggcgat gtcgcgaaaa ctcaatttcg cggaaatgct gagatcgcgg tccgaaggcc
      361 gcgatctatt gtttccaaaa tgaagcggtg acctacctgc cggggccggt agcgatggat
      421 tggctaccga tggtccggat gccctcttcg taggaggtgg gttggaagga gtaagccgtc
      481 tcaaacttgg tcgagtcgaa caggtaatcg aactcgtact ggtaaagcat ttcgtaaagc
      541 tcggcagtcg tccggtcaaa aagcccgcct aatctgacca tccatttttg caatacggtg
      601 tagcggcccg cccgatgcag ggaggctgcg gcgcggtcaa tgaactcggc ccccgtcagc
      661 ggctggcttg ccgtcggcag gtgccaaacc tggttccagg cactctcgtc cattgcaagg
      721 atcgccagtg cctttgcggc gtccggaaca taagtaaaag aatgccgggc atgtgcgttt
      781 accagccact gcgctttttg accttttgcg aggcgttgga aaaccaggag gttggggatg
      841 ctcgtcttgt ctgccgccgg gccgtaaaaa tcggcggatc gggcgatggc tgccgttata
      901 ttccctttcc ggacctcact cataagctgc gttgcaaggc gggcccgcag gtcgcccttg
      961 cggctactgg ggttataggg cgtctcctcc gtcatgggac cggacgtttg tccatacatg
     1021 tagacattgt cgaaaaacag aagtttcgcg ccggtctggc ggcaggcgcc gatcaggttg
     1081 tcgacgatgc ggggccattg ctgccgccaa atcgcgtaac ggtaggggag gccgatgcag
     1141 caataaacca cggaagagcc ccggatcgct tccagcgttt gggcggggtc cgtgcaatcc
     1201 gccggaaacg tggacgtgcc gggaattgcg gcgggactcc gggataccag gcgaaccgcc
     1261 agtttgcggc cagccagctc gcgggccagt gaatcagcca cgatgccatt ggcgcccaga
     1321 atggtatgga tggtggacat attgaaccgg caaagctaat aaatgacccg caggcacgaa
     1381 atttattctc cgttttccta cttttgcgat gtccctttat tgattaaacg tttgtttaat
     1441 tcaaacgttt gtttaaattt gtgcccttgt ttgaccttca ctagccaatg tacagcgtat
     1501 gtctataaat aaagcctggg ttacaggcct gatcgtggcg ggatgtctga cgggaatgcg
     1561 ggcccgtgct caaaaggcat cggataccct gcccgaagtc gcaaccgtgg atgcctgcgt
     1621 acaattcgcg ttgaaacatt acccgctcgt gcaacaagcc atgttggacg aacagatcac
     1681 ggaacgacag atcaggagca agctcgcgga ttggtatccc caactcgggc ttaacgccta
     1741 ctatcagaat aattttcaat tgcccgcttt attgttcaag ggcgctatcg tctacagcgg
     1801 aacctacaac caatcgcagg tagcactggg gctgacccag accttgttca accgggatgc
     1861 tttgctggcg agccagacca aacgggacgt gatgacgcag atccatcagt atacgatcgg
     1921 cgacaagatc gacgtggcgg taaacgtcag caaggccttc tacgatgtgc tgctcacaga
     1981 gcaggatatc aaggtactgg atgacgatat cgtccggctt gagcgtaacc tcaaagatac
     2041 ctacaatcaa taccagggcg gcctcgtgga caagaccgat tacaaacgtg cgatcatctc
     2101 gctcaacaac agcaaggcgc agcgcaagac cgacgaagag accctaaagg ccaaatatgc
     2161 cgcgctgctg caattgatgg gatataccgg cagggattcc ctgcacctgg tgtatgacag
     2221 cgcggccatg gaacaggagg ttgcgctgga caccaatgcc gtcgtcgact acggccaaag
     2281 gatcgaatac caggagctga tgacgcagaa gcggctgcag ctggccgacg tgaaatatga
     2341 aagatggtcc tatcttcctt cggtatcggc gggagccgga tacagcctca actatcttaa
     2401 ccccgaattc agcaagttat attcggataa ttttccccag tcgtatgcgg ggctcacggt
     2461 cagcgtcccg atcttccagg gcaccaaacg aacgcaaaat atccgcatcg cggagctgca
     2521 actggaacgg atcgactggg acgtcatcaa cctgcaaaat cagatcaata cccaatatac
     2581 ccaggcgctg gctacttatg agagcaattt gaacaactat cacgttcagc gcgacaattt
     2641 gggattggca agggaagtat tcaacacgat cga
//
LOCUS       DATGKE010000234         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_954898, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000234 DATGKE010000000
VERSION     DATGKE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954898"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VKU83_04060"
     CDS             complement(<1..1464)
                     /locus_tag="VKU83_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI92752.1"
                     /translation="MVESILTLEGVTVQAGAVKLLDELDWTIRKGEQWAVVGPSGGGK
                     TVLAHTLLGRHFSTGRIRTGEVRMTIVEQQHRFQGLPGASALYYQQRFNSQDAEGTIT
                     VGEELAAAGFQEAPPPATRESGGQAAGQPGPEAEWLDTLGVRSLLQKPLIQLSNGENK
                     RVQLAIAMLERPQILILDNPFLGLDAEGRSVLHTIVNHLAESGTQILLITSEREIPSC
                     ITHLARLEKGRWDWMGPRAGFEPAPVPAQLFQPDAEIMTRLKDGKSISDGDDAGEVVI
                     DMRGVNIRYGDALILESIDWQVRRGERWNISGPNGAGKSTLLSLITADNPQAYANKIW
                     LFGRRRGTGESIWDIKRRIGFVSPELHLYFDPGATCFEVVASGLFDTIGLFRTLSAGE
                     EEAVMRWISVLSLQQLRSKRLVQLSAGQQRMVLLARALIKDPPMLILDEPCQGLDEWQ
                     TAYFTDVITVLCEGFDRTLLYVSHYTRDLPRCIDKFLR"
     gene            1764..>2751
                     /locus_tag="VKU83_04065"
     CDS             1764..>2751
                     /locus_tag="VKU83_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92753.1"
                     /translation="MAEKANLFEYNEESVKTLDWREHIRLRPGMYIGKLGDGSSPDDG
                     IYVLLKEVIDNCIDEYTMGYGKQVDITVEQKTVNIRDYGRGIPLGKVVDVVSNINTGA
                     KYDSKAFQKSVGLNGVGTKAVNALSSYFKVTAFRDGREKSAEFAKGVLTKDYKEGKTD
                     QPNGTLVTFIPDETVFKNFHFIHEYMDNQFWNYCYLNAGLVIQFNGKKYVSRNGLLDL
                     LQRRTNEDELRYPIIHLKGDDIEVAIAHNNDYGEEIFSFVNGQYTTQGGTHQQAFREA
                     FVKVIRDFYKKDFEAADIRQSIVAAVSVRVVEPVFESQTKTKLGSQYMQEGGA"
BASE COUNT          622 a          778 c          765 g          586 t
ORIGIN      
        1 cctgaggaac ttatcgatgc agcgtggcag gtcccgggtg taatggctga cataaagcag
       61 cgttctgtcg aatccctcgc aaagaaccgt gatcacgtcc gtgaaatagg cggtctgcca
      121 ttcgtccagg ccctggcacg gctcatccag gatgagcatc ggcggatctt tgatgagcgc
      181 acgcgccagc aagaccatgc gttgctggcc tgctgaaagc tgaacgagcc gcttgctcct
      241 cagctgctgc agggacaaga ccgatatcca tcgcatcacc gcctcctctt ccccggcgct
      301 gagggtgcgg aagagcccaa tagtatcgaa aagtcctgac gctaccactt caaaacaagt
      361 tgcgcccgga tcgaagtaca aatggagttc gggcgacacg aagccgatcc ttcgtttgat
      421 atcccagatg ctttctccgg ttccccgccg ccggccaaag agccagatct tattggcata
      481 ggcctgcggg ttgtccgcgg tgatgaggct caggagcgtt gacttacctg cgccgttcgg
      541 cccgctgata ttccagcgtt caccgcgccg gacctgccag tcgatacttt ccagaataag
      601 cgcgtcgccg taccggatat tgacgccccg catgtcaatg accacctcgc cggcatcgtc
      661 accgtccgag atcgattttc catccttcag ccgggtcatg atctccgcgt ccggctggaa
      721 caattgggcc ggtaccggcg cgggctcgaa tcctgccctg ggtcccatcc agtcccaacg
      781 gcccttttcc aggcgggcga gatgggtgat gcaggagggg atttcccgtt cacttgttat
      841 aagcaagatc tgtgtcccgc tctccgccag gtggttgacg atcgtatgca atacgctccg
      901 tccttcggcg tccagcccga ggaaaggatt gtcgaggatc aatatttgcg ggcgttccaa
      961 catcgcgatc gccaactgga cccgcttgtt ctcgccattc gagagctgga tcaggggctt
     1021 ttgcaaaagt gaccgtacgc cgagcgtgtc cagccattcc gcttcaggtc cgggttgtcc
     1081 ggccgcctgt ccgccacttt cccgtgttgc cggcggcggc gcctcttgaa accccgctgc
     1141 tgccagttct tcgcctacag tgatggtccc ctccgcatct tgtgaattga atcgctgctg
     1201 gtagtaaagc gcggaggcgc cgggcagtcc ctggaaccga tgttgttgct cgacgatcgt
     1261 catccggact tcccccgtcc ggatccggcc ggttgaaaaa tgcctgccca acaaggtatg
     1321 cgcaagcacc gtctttccgc cgcccgatgg tccaaccacg gcccattgtt cgcccttccg
     1381 gatggtccaa tccagttcgt cgagcagctt cacagcacct gcctgtaccg tcacgccttc
     1441 cagtgtcaaa atgctttcca ccatcctgcg aatgtaaggc cgcgacgtga aacttgttcg
     1501 gggtggccgg gggtgcccgg ggcatgtaca agggttaatt gggccgaatt cggcccaatt
     1561 aatgggagaa ggcaggcggg gggcagggag ggacctccgc caatatccac aactgtagaa
     1621 atccaataat ccgcctctga tttcgtttgc ctatcttgca gacttaactg agggggtgaa
     1681 cccggttggc caaacattaa ctatctgggg atgaaggtga aatgtgctca ttgccggaat
     1741 cgccaggcta tcgtgaccct tttatggcag aaaaagctaa tttgttcgaa tacaatgaag
     1801 aaagcgttaa aaccctcgat tggcgggaac atatccgcct gcggcccggg atgtatatcg
     1861 gaaaactggg cgatggcagc agtcccgatg atggcatcta tgtactcctg aaggaagtga
     1921 ttgacaattg tatcgacgag tatacgatgg gttatggcaa acaggtggac attaccgtcg
     1981 aacaaaaaac agtcaatatc cgcgactatg gccgcggcat tccccttgga aaggtcgtgg
     2041 atgtggtcag caatatcaat accggcgcca aatacgacag caaagccttt cagaaatccg
     2101 tcgggctgaa cggcgtcggg acgaaggctg tcaacgccct cagcagctat ttcaaggtga
     2161 ccgcatttcg cgacggccgc gaaaaatcgg ccgaattcgc caaaggcgtc ctcacgaagg
     2221 actacaagga aggcaagacc gaccagccga acgggacgct tgtaacgttt atccccgacg
     2281 agacggtatt caagaacttt cactttatcc atgagtatat ggataaccag ttctggaatt
     2341 actgctatct gaacgcggga ctggtcattc agttcaacgg gaaaaaatac gtttcgagaa
     2401 acggactgct ggaccttctc cagcgtcgca caaatgagga tgagcttcgt tacccgatca
     2461 tccacctgaa aggcgacgat atcgaagtgg ccatcgcgca taacaacgat tacggggaag
     2521 agatcttcag cttcgtgaac ggccaataca ccacccaggg aggaacgcac cagcaggcat
     2581 tccgtgaagc tttcgtcaag gttatccggg acttctacaa aaaagatttc gaggcagcgg
     2641 atatccgcca aagcattgtt gccgccgttt cggtgcgcgt ggtcgagccg gttttcgaat
     2701 cgcaaacgaa gacgaagctg ggaagccagt atatgcagga gggcggggcg t
//
LOCUS       DATGKE010000235         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_789625, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000235 DATGKE010000000
VERSION     DATGKE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789625"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VKU83_04070"
     CDS             <1..1589
                     /locus_tag="VKU83_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92754.1"
                     /translation="QPLLAIHRGNIPIFLESSNISNPAYSYILGGIASFILLIACINF
                     INLTIARSIKRSKEVGIRKIIGGSRFKLVTQFLTESFLACSIAFLLAIGLALLALPVF
                     NDVMNKQLSLSYLLDVRLILESIGFLLVTGLAAGFYPAMVLSGFNPLDTLYQRLRVTG
                     GAILAKALVVVQFALAGIFIICAFFISRQVHFLAHKDLGYNDADLVTVNIDADNGQRF
                     ARLFRNELLRNPAILAVARHNGQGRMEIAQAEGKQILFSYEHIDENYFRTLQIPLVMG
                     RNFSPEFPADSLHSIIVNETFAQNAGWKNPIGRTIEFPANHQRMTIVGVVRDFNYWPL
                     TMNIMPQLFSIEPAGDDLEFDIRIAPADKAETLAFIQKVFSTLAPYYPFSYTFRQDDN
                     RHAYDAQEKWQAIITFAAIFTAFLSCIGLFGLTLLATERRIKEIGIRKVLGASVAQLV
                     RLLSLGFIALVILANFIAIPLAWWAMDKWLQNFAYRISLQWWVFALALLMSVLLAFAT
                     MALRAVKAATASPATNLRAE"
     gene            1660..>2860
                     /locus_tag="VKU83_04075"
     CDS             1660..>2860
                     /locus_tag="VKU83_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLI92755.1"
                     /translation="MKRRKFIHDNLAAGAGMVLSPALARLPVPGFAAAKKKVIIAGAG
                     ITGLCCGYELMKLGHEVTILEASGRYGGHVYTGRDGLSDGLYADFGADHITKPGYEKF
                     FEYAAEFNLTPIPYPNAEGSDAAYDKDKRSMIGGKWYTAGQLHDPVVLKAFGFNDKEA
                     QFLTTGDFDGLRDLYLHPYLDKFTDPYQPFGVGYDDFDHLPVADLYKKEGASAAALRF
                     MGGRNTNALYALWRLSLMHSRGIPLNEGETFHLKDGNEQLPIAFASRLGHRVLLNHPV
                     TAVHHNPQGVTVTYRPYNKTEEQLSADALVVCITLPVFRNIPVTPALSPEKQFVVDNL
                     GYSSHPFYVFEAASKFWLDDGIPSINMEFEHPDISGIWSETNQVQTERVILKAYGPGG
                     LSPQRVLA"
BASE COUNT          613 a          862 c          718 g          667 t
ORIGIN      
        1 tgcagccgct gttggcaatc catcgcggca acatcccgat ctttcttgaa agttccaaca
       61 tcagcaaccc cgcttatagc tatattttag gtggaattgc cagctttatc ctcctgattg
      121 cctgcatcaa ttttatcaat cttaccatcg cccggtccat aaaaagaagc aaggaggttg
      181 gcatccggaa aatcattggc ggaagccgtt tcaagctggt cacgcaattc ctcaccgaat
      241 ccttcctcgc ttgcagcatt gcgtttctcc tcgcgatcgg acttgccctg ctggctttac
      301 ccgttttcaa tgacgtgatg aacaagcagc tcagcctttc ctatctcctc gatgtccgac
      361 tgattctgga atcgatcgga ttcctgctgg tcactggtct tgccgccggg ttctatcccg
      421 cgatggtcct gtccggtttc aacccgctgg acactctcta ccagcgcctc cgggtaacgg
      481 gcggtgccat tctggctaag gcgcttgtgg tcgttcagtt tgccctcgcc gggatattca
      541 tcatctgcgc cttcttcatt tccaggcagg ttcatttcct ggcacacaaa gacctcggct
      601 acaacgacgc cgatctcgtc accgttaaca ttgacgccga taacggtcag cgattcgccc
      661 ggctgttcag gaatgagctg ctccgcaacc ccgccatcct ggccgtggcc cggcacaatg
      721 gacagggacg gatggagata gcccaggccg aaggaaagca aatcctcttt tcttacgaac
      781 atattgatga aaactatttc agaaccctgc aaattcccct ggtcatgggc cggaattttt
      841 cgccggaatt cccggctgac agcctgcact cgatcatcgt gaatgaaaca ttcgctcaaa
      901 acgcggggtg gaaaaacccg atcggaagaa cgatcgaatt tccggccaat caccagcgca
      961 tgaccattgt cggcgtcgtc cgcgacttca attattggcc gctcacaatg aacatcatgc
     1021 cccagctctt ctcgatcgag ccggccggcg acgacctcga attcgacatc cggatcgcac
     1081 cggccgacaa ggccgaaacc cttgccttca ttcaaaaagt cttttcaacg ctcgcccctt
     1141 actacccttt cagctatacg ttcaggcaag acgataaccg ccatgcgtac gacgcgcagg
     1201 aaaagtggca ggccatcata acctttgccg cgatattcac ggcgttcctc tcctgcatcg
     1261 gcctctttgg cctgaccctg ctggcgacgg aacggaggat caaagagatc ggcatccgga
     1321 aggtgctcgg cgcgtcggtc gcacaactcg tcaggttgtt gtcactcgga tttattgctc
     1381 ttgtgatact ggccaacttc atcgcgattc cgctggcctg gtgggctatg gataaatggc
     1441 tgcagaattt cgcctaccgg atctccctgc aatggtgggt attcgcgctt gcgctgttga
     1501 tgtccgttct ccttgcattc gcgacgatgg cgttgcgtgc cgtgaaagcg gccaccgcca
     1561 gtccggccac caatctgcgg gcggaataac cttgaggctt cccgcaatgc ccggctttac
     1621 gaaaaaccct taaattgtgc ggcacatccc tgcacgccaa tgaaaagacg aaaattcatc
     1681 cacgataacc ttgcggccgg cgccggcatg gtgttgtccc ccgccctggc caggctgccc
     1741 gtccccggct ttgccgccgc taaaaagaag gttattattg ccggcgccgg tattaccggc
     1801 ctctgctgcg gatacgaact aatgaagctg ggccatgagg tgacgatcct cgaggcttcc
     1861 ggcaggtatg gcggtcatgt atatacgggc cgcgacggac tctccgatgg gctgtatgcc
     1921 gacttcggcg cggatcatat cacgaaaccg ggttatgaga aattcttcga atacgccgcg
     1981 gagttcaacc tcacgcccat cccctatccc aatgccgagg gatccgacgc agcctacgac
     2041 aaagacaagc ggagcatgat cggtggaaag tggtacaccg ccgggcaact gcatgatccg
     2101 gttgtcctga aagcattcgg ctttaacgac aaggaagctc aatttcttac aacaggcgat
     2161 tttgacggcc tcagggatct ttacctgcat ccttatctcg acaagttcac ggacccttat
     2221 cagccctttg gggtcggcta tgatgatttc gatcatcttc cggtcgccga cctgtataaa
     2281 aaagaagggg cttctgccgc cgccctgcga ttcatgggtg gccggaacac caacgccctg
     2341 tatgccctct ggcggctttc gctcatgcat tcccgcggca ttccccttaa tgaaggcgag
     2401 acgttccacc tcaaagacgg caatgaacag ctaccgatag ccttcgcctc caggcttggg
     2461 catcgtgtcc tgctgaatca tcccgtcacg gctgtccacc ataatccgca gggagtaacg
     2521 gtgacctacc gtccctataa taagaccgag gaacaactaa gcgccgatgc gctcgtcgtt
     2581 tgcattaccc tgcccgtctt tcggaacatc ccggtaacgc ccgccttgtc gccggaaaaa
     2641 caattcgtcg tcgataacct cggctactct tcccacccat tttatgtttt tgaggccgcc
     2701 tcgaaattct ggctggatga cggcattccg agcatcaata tggaattcga acatccggat
     2761 atttcgggaa tctggagtga gacaaaccag gtgcagactg aacgggtcat tctgaaggct
     2821 tatggtcccg gcggcctttc gccgcagcgg gtgctggcag
//
LOCUS       DATGKE010000236         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_165279, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000236 DATGKE010000000
VERSION     DATGKE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165279"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..3359
                     /locus_tag="VKU83_04080"
     CDS             324..3359
                     /locus_tag="VKU83_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92756.1"
                     /translation="MRCAVLLVLILSRCGMAFCQQPYLFQHITPEDGLFANSNINVYE
                     DKEGYYWFSSVQGLQRYDGRGLVTYHYLYKLRRDFSDNIAVRPTEDREGNIWVWNGEG
                     IYILNKKKARLERLYLEDASDSNVSNVCNVLKAKDERLWIVTSRNIFVYNDSLRRPVL
                     LCPGAGDAMHAVYDRQRNGIWIILASEPHAFVFFDCASRHLTCPGDMSLDRLFGYFNP
                     LSLLKFDRNNYLWIADYLGDLCRYDPNSGQATFFDILHQKRWGNSQTPNSAIFDFVDD
                     GASIWFSSNNYTGILRFSGTDGSFVSIMNDNVNQSGLHFQNECNNMLLDREDNIWVNT
                     DMGLNIFNPKRQRFKYLSSSDPAHFQPFSQDVTSFLQTSNGEIWITTWGSGVFRYDSN
                     FRLLQNYIHRDHDPTSLGEPLNKTWSLAEMPGGKLLVGCQYAVLAELDPKTSRFNNRI
                     VAPFNKRTIIVMTGDKTNKFWFGLHSGVLGRWDPGEDKMITIHNLYGEKDKVIHAIDG
                     LFTDKDGMIWCAAGDDAVKCIDPRTFAVQSCIRGIHPLRLSAFGDSLIIGGSFGDGLF
                     VYNKFTKGLRFFTTENGLSSNIVYSAVADADQNIWVVTNESIERLNLPTGKVIRFGPE
                     DGIRDHVFQRAAYQLKNGTILVAANSGVIYFNPAGFRSDPPPRPVTITGISVGQQDLP
                     VDSLEPEGLSIHNNPLAISFGSLSFCERNNISYFYRLDGADTGWVATGKNRSVLYANL
                     APGHYRLQLKCQNREGMMSPITQLAIVILPPWWETWWAWLLWTLLALSVAWSVYTYHR
                     RTSRQLADVRQKIASDLHDDIGSTLNSISVYSEVARQQVDSNKDNAIQLLKKMGVASR
                     GMIDNMNDIVWAVNPKNDQFENVVERMQFFAAELLSGKNILLEFHIDERSKKARLTME
                     KRKNFYLIFKEAVTNSYKYSHAKNVTVQIAAEGGWLVMRISDDGSGFDLAGRGGSGSG
                     SGNGLRNMQSRSKEIGAELTVSSAPEGGTTVQLKMGISR"
     gene            3365..4000
                     /locus_tag="VKU83_04085"
     CDS             3365..4000
                     /locus_tag="VKU83_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92757.1"
                     /translation="MSKDINVAIFEDNYLLRDGYFQLINGTPGFSCVGAFDSATDLEF
                     KLRRCQPDVVLMDIDMPVLNGIEAVKLIKARFAGIHVIMQTVFEDEDKIFHAILAGAE
                     GYILKKSPPARMLEAIAEVYNGGAPMTPSVASKTLKLFRSGVRPLPAVKDQGLTDRQK
                     EILECIVSGMSYKLIADKLVVSVDTVRYHVKNIYQILHVHSRFELLNKQKR"
     gene            4104..4574
                     /locus_tag="VKU83_04090"
     CDS             4104..4574
                     /locus_tag="VKU83_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92758.1"
                     /translation="MHEPHYACILLDITLPNGNGLNILGELRRLGKAEGVLIISARNS
                     PDDKVRGLQLGADDYLNKESIVDHLWGEQIDMADNYDFIYTHVSNLRKKLSQAGCKDY
                     IRAVYGMGCHLELATIKLPNVLDLRLLLAQTMKTSMPPDETVRLPERLAFTQPP"
     gene            complement(4571..>5011)
                     /locus_tag="VKU83_04095"
     CDS             complement(4571..>5011)
                     /locus_tag="VKU83_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92759.1"
                     /translation="KTLFMMHWRRISLAILLLLGACKKDNNNSSSANGWIDGFWTYKE
                     DVNVDYWNANVLFKSDGTFRMYQALTLDDTAAGPALADTPNQVVTFGTYTVSGTKVTM
                     KYTEFNVVDFTFNGSVNSAHNVLIGNLEDNNPGDAQPLWYLTKP"
BASE COUNT         1260 a         1285 c         1302 g         1164 t
ORIGIN      
        1 gccttcgcat tggcctgtgc gaacatctgt aaacgttaac attgatccct gtagcccgat
       61 cttgttgatc gttccgctga gttgtttttg taacgtgtcg ccagttgaat aaagcgaaaa
      121 tttaatactg gatgatccgc cattgatggt caaaatattc agttgattca tgctactcat
      181 ataatttttg ttttttcatg attggctggg ggcaaaggta gcatcactta atgtcgttcg
      241 caacatctac aagctgaggg gagctttcaa gaagatttgg ggattgagga aatatccatt
      301 aacttattat ttaaattgaa cccatgcgat gcgccgtgtt gctcgtgctg atcctgtccc
      361 gctgtgggat ggctttctgc cagcaaccgt acctgtttca gcatatcacc cccgaagacg
      421 gactttttgc caactcgaat atcaacgtat acgaggataa agagggttat tattggttca
      481 gctcggtaca aggcctgcag cggtatgacg gccggggcct ggtcacttac cattatctct
      541 ataagctccg cagggatttt tcggacaaca tcgcggtcag gccaaccgaa gacagggaag
      601 gaaatatctg ggtctggaac ggggaaggaa tctatattct caataaaaag aaggccaggc
      661 ttgagcggct gtacctggag gatgcatcgg acagtaatgt cagcaatgtc tgcaatgtcc
      721 tgaaggcaaa ggacgagcgc ctttggatcg tgaccagcag gaatatcttt gtctataatg
      781 acagcctgcg ccggccggtg cttctctgtc ccggcgccgg ggatgccatg catgccgtat
      841 atgatcggca gcggaacggg atctggatca ttctcgccag cgaaccgcat gcgtttgttt
      901 tttttgactg tgcgtcccgg catttgacct gcccgggcga catgagcctg gaccggctat
      961 tcggctattt caacccgctc tcgttgctca aatttgaccg caataactat ttatggatag
     1021 cggactatct tggggatctt tgccgttacg accccaacag cgggcaagcc accttttttg
     1081 acatccttca tcaaaaacga tggggtaact cccaaacccc caattccgcc attttcgatt
     1141 ttgtggatga cggcgcatcg atctggtttt cgtccaacaa ttataccggg atactaagat
     1201 tctcagggac cgacggcagc ttcgtatcga taatgaatga caacgtcaat caaagcgggc
     1261 tgcacttcca gaacgaatgc aataatatgc tgctcgaccg agaggacaac atctgggtga
     1321 acacagacat ggggctgaac atctttaacc caaaacgaca acggttcaag tacctcagca
     1381 gctccgaccc agcccacttc caacccttca gccaggacgt gacatccttc ctgcaaacct
     1441 ccaacgggga aatctggatc actacctggg gcagtggagt tttcaggtat gacagcaatt
     1501 tccggctgct ccaaaactac atccaccgcg accatgatcc cacctcattg ggcgagcccc
     1561 tgaacaagac atggtcactc gccgaaatgc ccggcggcaa actccttgtc ggctgccagt
     1621 acgccgtgct cgcggagctg gatccaaaga ccagccggtt caataaccgg atcgtagcac
     1681 ccttcaacaa acgaaccatt atcgttatga ccggcgataa aacaaataaa ttctggttcg
     1741 gattgcacag cggtgtgctc gggaggtggg atcccggaga ggataaaatg atcacgatcc
     1801 ataacctgta cggggagaag gataaagtca tccatgcgat cgacggacta ttcaccgaca
     1861 aggacgggat gatctggtgc gccgcgggcg acgatgcggt gaaatgcatc gatccgagaa
     1921 ccttcgcggt tcaaagctgt atccggggaa ttcatccgtt aaggctgtcc gcgttcggag
     1981 attcgctgat catcggcggc agtttcgggg atgggctttt tgtctacaac aaattcacga
     2041 agggtttgcg cttctttact actgaaaacg gcctgtcgtc caatattgtc tactcagcag
     2101 tggcggacgc cgatcagaac atctgggtag ttaccaacga gtcgatcgag cgcctgaacc
     2161 tcccaaccgg caaggtcatt cgctttggtc ctgaggacgg cataagggat catgtttttc
     2221 agcgggcggc ttatcagcta aagaatggca ccatattggt agccgccaat tctggcgtga
     2281 tctatttcaa tccggccggc ttccggtccg atcccccacc ccgcccggtg accataaccg
     2341 ggatcagcgt aggccagcaa gacctgccgg tcgattcgct ggagcccgag gggctttcga
     2401 tccataacaa tccgctggcg atctcgttcg gatccctttc cttttgcgaa agaaataata
     2461 tttcctattt ctaccgcctt gatggcgccg ataccggctg ggtggctacg ggaaaaaacc
     2521 gatcggtact ctatgccaat ctggcacccg gtcattaccg gctgcagctg aaatgccaaa
     2581 atcgcgaagg gatgatgtcg ccgatcacgc agctcgcgat tgtcatcctg ccgccctggt
     2641 gggaaacatg gtgggcctgg ttactgtgga cgctgctcgc cttatcggtc gcatggtcgg
     2701 tgtataccta tcaccggagg acaagccggc aattggcaga cgtccgtcag aagatcgctt
     2761 ccgatctgca cgatgatatc gggtccacgc tcaacagcat ctcggtttac agcgaagtgg
     2821 cccggcagca ggtcgatagc aataaagaca atgcgataca gctcctgaaa aaaatgggcg
     2881 ttgcgtctcg cggaatgatc gataatatga atgatatcgt gtgggccgtt aaccccaaaa
     2941 acgaccaatt cgagaacgtc gtagagcgaa tgcagttctt cgccgccgag cttctttcgg
     3001 gaaagaatat cctgctggaa ttccatatcg acgagcgttc aaaaaaagcc aggctcacca
     3061 tggagaaaag aaagaacttc tacctcatct ttaaggaggc ggtgaccaat tcgtacaaat
     3121 attcccatgc caaaaatgta acggtgcaga tagcggcgga gggcggctgg ttggtcatgc
     3181 ggataagcga cgacggctcg ggattcgatc ttgcagggag gggcggatcg ggaagcggat
     3241 cagggaacgg acttcgaaat atgcagtcga gaagcaaaga gatcggcgcc gagttgacag
     3301 tatcgagcgc gccggaaggg ggtaccaccg tacaacttaa aatggggatc agccgataat
     3361 tattatgtca aaagacatca atgtcgcgat ttttgaagac aattacctgc tgcgggacgg
     3421 gtactttcag ctgatcaacg gaacgccggg tttttcttgc gtcggcgcat tcgacagcgc
     3481 taccgacctt gaattcaaac tgcgccgctg ccagccggat gtagtgctga tggatatcga
     3541 tatgcctgta ctgaacggaa tcgaagcggt caaactcatc aaagcccggt ttgcggggat
     3601 acatgtcatt atgcaaacgg tattcgaaga cgaagacaag atattccacg ctatcctcgc
     3661 gggcgccgaa gggtatatcc tgaaaaagag cccgcccgcc cggatgctgg aagccattgc
     3721 cgaagtctac aacggcggag cgccgatgac acctagcgtc gcctcgaaaa cattgaagct
     3781 gttccgctct ggcgttcggc cattgccagc ggttaaagat cagggtctga cggaccggca
     3841 aaaggaaatc cttgagtgta tagtaagcgg gatgagctat aagctaattg cagataagct
     3901 ggttgtcagc gtcgatacgg tgcgctatca cgtcaaaaac atctaccaga tccttcatgt
     3961 tcattcccga ttcgaattgc tgaacaagca aaagcgataa tcctacccgc caatgggata
     4021 cccgcgccgt agctcatgaa gatttggaga ttgcgggaat atagccgaat ttccatgtct
     4081 taagaacacc tgccttaaag aatatccatg aaccccacta cgcctgtatt ctgctggaca
     4141 ttaccctgcc gaatggtaat ggcctcaaca ttcttggcga gctcaggagg cttggcaagg
     4201 ccgaaggggt cttgatcatt tctgcaagga attcgccgga cgataaggtg cggggtctgc
     4261 aactcggggc agatgactat ctgaacaagg aatccatcgt ggatcattta tggggagaac
     4321 aaatcgacat ggccgacaat tacgatttta tctacacaca cgttagtaac ctccgaaaaa
     4381 aactatctca ggccgggtgt aaggattaca tcagagcggt gtacggcatg ggctgtcatc
     4441 tggaacttgc aacgataaag ctaccaaatg ttttggacct ccggctcctg ctggctcaga
     4501 caatgaaaac ttccatgccc ccggatgaaa cggtgagatt acccgagagg ctggcattca
     4561 cccaaccacc ctaaggcttc gtcaaatacc aaagcggctg cgcgtcgccc gggttgttgt
     4621 cttcaagatt cccgatcagg acattgtggg cgctgttgac cgacccgttg aacgtgaaat
     4681 cgaccacatt gaattcggta tacttcatcg tcaccttcgt cccgctcacc gtataggtgc
     4741 cgaacgtcac gacctggttc ggtgtatcgg cgagcgcagg ccccgcggcc gtatcatcca
     4801 gcgtcaacgc ctggtacatc cggaacgtgc cgtcgctttt gaacagtaca ttggcattcc
     4861 agtagtccac atttacatcc tctttgtagg tccagaaccc atcgatccag ccattggcgg
     4921 aagaggaatt gttgttgtcc ttcttgcatg ctcccagaag cagcagtatc gctagcgaaa
     4981 ttcgtctcca atgcatcata aaaagagttt t
//
LOCUS       DATGKE010000237         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1246009, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000237 DATGKE010000000
VERSION     DATGKE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246009"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VKU83_04100"
     CDS             complement(<1..1238)
                     /locus_tag="VKU83_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI92760.1"
                     /translation="MTLIILESFAFFASLYLLKILIDRVASNAIGGRRDVIYYIIAAG
                     AASAAYVIIKALSAYVTEIYAATLAESIDDKIHARAIELDFAFYESPAYFDILKRARE
                     AGPDGPKAIVSNLADIAKNVMSLVAIAFILFAIDWFLLPLLVVFITPTLLVKIYFADQ
                     LHQRRLRQTALERRASYMSSLITSDGAAKELRSFGIGDHLRTLYRKIRISILAERFLI
                     SRKSTWSEILTTVLATAGFFSAIAFIAIRPKAGETKAGDITLFLIAFSQGFSMLQGLA
                     SGISRLYQNSIFVSGLFELFDLKPTMKDKESPAALPSDHRPTLEVKDVSFTYPNTTDP
                     VLRNISMRIPAGKIVAVVGLNGAGKTTLIKLLTRLYDPDSGAICLGGVDIRDLRASEY
                     RRKVCTVFQDFERYHFTVKE"
     gene            1608..2486
                     /locus_tag="VKU83_04105"
     CDS             1608..2486
                     /locus_tag="VKU83_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HLI92761.1"
                     /translation="MKTAHLILAYKNPYQVRRLARVLTACPEFDCWVHIDKKVDIELF
                     APFLHLPRVRFIRNRVPVNWGGYSCLEAAFNGLREIIESKEDYDFVNLLSGQDYPIKP
                     VDEINDFLSANRNKSFLAVETEPSAWWEHAIGRLTKYHFTDFGFKGKTVLQQLMNRLM
                     PDRQFMQSFSLYGGPYSSYWTLSMQAAAYVHDFVENNKKLVDHCRYTWAPDEFLVSTI
                     LMNSPLRDELVNKSLRYIDWSEGGGHPRILNASDFGPLMNSSCLFARKFDMEADPKLL
                     TKVDTLLLGQTIDTLS"
     gene            complement(2467..4863)
                     /locus_tag="VKU83_04110"
     CDS             complement(2467..4863)
                     /locus_tag="VKU83_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLI92762.1"
                     /translation="MLRHYQNLLLAGCLLLVTAADAQTSMNELAAQFIEHIRTGRKEK
                     LMVLTDKPYYAAGEKIWFNAWCLDSLSNRFLYLSKNLYVDLVNDQDSVISQLLFNIPA
                     HKTAGMILLPATLPEGYYWLRAYTTRMLTEDSSRIFVRPVYVVNINKPNPGALSAYAP
                     KTVPVGTDTAAPSLLFYPEGGSIISGTTATVGFRALGSRGQPVDVSGYVTDPLHDTVA
                     RFHSVLPGTGKFSFDAYNPRKYTAHVSWRGRELLFPLPALNQFAYQLSVIGRDDRIIH
                     LQVSLGDSLYKKNKSTKLLAVSRDSLCFAANGTDMYQVNVPVAALPPGKVSFYLFDDR
                     DRMVSQRSVYVPGRDSGRLQAATDKAHYGPGEKVGLSIGVRAEDNHPQTATLLAVAVT
                     DDRLTGDRSLNAELGEVAPFGTAPADLAGNAGDTGEDVLMLTAAPSYSGWKYLPGETR
                     TVNLPRFVDSNLLNIRGKAIGNDNAPLQHYIVNLVSGDNRFFMADTTDTQGRFSFPLT
                     EYDDGTRFNMKLTTLQGKAATGKLIIDKMDYPAFRTPAVLKQGLGKDEIAIIRRYRSR
                     ESSPDLAQLKDTGLLKPATVKGRSRLPPEDEAKRASSFSDVIGPDQLHKGGVDAIKDA
                     LASVPGLTSAINRVVGLGTADPVLVLLDGVPQNAAIIESLDASNIEFIEVLKGPLTAV
                     YGMQGSGGVILINSVNKSEAVAQTNAKGLTTIYPRGYYSRPNVYASGWDPAKGPGPGD
                     GTVDASTLYWNGDIVTDNNGKAKVDFFTGRQQAVYSAAIVGITMAGQIVEGKIKIRCQ
                     "
     gene            complement(4903..6102)
                     /locus_tag="VKU83_04115"
     CDS             complement(4903..6102)
                     /locus_tag="VKU83_04115"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HLI92763.1"
                     /translation="MKLQLFSLALALGCFAPAIHADGQAYLKLHDKAIVADTHNDVLS
                     TATMNGMDIGSDLTGKTHSDLARFKKGGVDVQVFSIFCDDHYGVGSAFHMAHREIDSL
                     YAIARRNPDKMEIVTDPAGLESAVRHHKLAAMMGVEGGHMIEDRMDYLDSFYRLGVRY
                     MTLTWNNSTPWATSAMDETGRAFSVTPYGLTAEGRAIVRHMNELGMMVDLSHVGEKTF
                     WDAIHTTTKPVICSHSSVYALCPVFRNLKDDQIKAIGGNGGVIQVNFYSGFLDSNYAP
                     RLTRFLGRHKAELDSMNAAKTPQYAINEFLSRKYPAESDSLRAPLSLLMDHIDYIVRL
                     IGVDHVGLGSDFDGIESPPQQLNDVTCYPLITRELLKRGYKPSDVEKILGGNFIRVFK
                     ANCEAVK"
     gene            complement(6113..>6580)
                     /locus_tag="VKU83_04120"
     CDS             complement(6113..>6580)
                     /locus_tag="VKU83_04120"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HLI92764.1"
                     /translation="ITNQIKDSYLGITGTDRVSEYAAGRKAQLEHAKRITDSIWKEVE
                     AVQKGAGARGADWSAYTGDYRDNWLGDVTISVKYGRLWFDSRRSPRLTGEMLPFKGNS
                     FIVKWVDRSMDADAYVSFGLDERGIATEIRMKPVSPLTDFSYDFQDLDFHREK"
BASE COUNT         1433 a         1788 c         1810 g         1549 t
ORIGIN      
        1 ttctccttta ccgtaaagtg gtaacgctca aaatcctgga atacggtgca tacttttctt
       61 cgatactccg aagccctgag gtcgcgaata tcgactccgc ccaggcatat tgcccccgaa
      121 tcggggtcat acagccgggt caataattta atcagggtcg ttttgcccgc gccgttcagg
      181 ccgacgaccg ctacgatctt tccggcaggg atgcgcatgc tgatattcct aaggacgggg
      241 tcggttgtgt tgggataggt aaagctgacg tcctttactt ccagggttgg tcgatgatcc
      301 gacggcagcg ccgcagggga ttctttatcc ttcatggtgg gcttgagatc gaatagttcg
      361 aagaggccgg aaacgaaaat gctattctgg tatagtctgg agatccctga tgccagcccc
      421 tggagcatgc tgaaaccctg ggaaaaagcg attaagaaca gggtgatatc gccggctttg
      481 gtttcgcctg cttttggccg gatggcaatg aaagcgatgg ccgagaagaa cccggcggtt
      541 gcaagaaccg tggttaaaat ttcgctccat gtgctcttcc tgctgatcaa aaaccgttct
      601 gcaagaatgg agatgcggat tttgcgatat agggtccgca gatgatcacc aataccaaaa
      661 cttcgtagtt ccttggccgc gccatcggac gttatcaggc tgctcatata cgaggctcgt
      721 ctttcgagtg cggtttgccg gagcctgcgc tgatggagtt gatcggcaaa atatattttc
      781 accagcaggg tgggcgttat gaagaccacc aggaggggta gcaggaacca gtcgatcgca
      841 aacaggatga aggcgatcgc taccagtgac atgacattct tcgctatatc tgccaaattc
      901 gagacaatgg ccttgggccc gtcaggacct gcctccctgg cccgtttcag gatatcaaaa
      961 taggcgggac tttcataaaa cgcgaagtcc agttctatcg cccttgcatg gatcttgtca
     1021 tcgatagact ccgcgagggt cgccgcatag atctcggtta cataggcgga taaggctttg
     1081 atgatgacgt aggccgcgga tgcggccccc gcggcaataa tataatagat cacgtcgcgc
     1141 ctcccgccaa tcgcgtttga cgcaacccgg tcgatcagga tcttcagcag atagagggag
     1201 gcgaagaacg caaagctttc gagaatgatc agggtcatgc tccataccgc ccatttcctc
     1261 ccagtcgaga agaccagttt taggatccgc gcaagctgaa gcctgctcct aagctgccga
     1321 aagcggttta gaaggtgtgg gtgcatgggg agtagagact atgtaatgta cagtaaaaat
     1381 cgcaggaatg atcgttcgcg catcccgcat ttatatcttc cttgctgatt ctcaaatgaa
     1441 taacatgcgc gcatcagcct ggaccccggc gcggcctgaa agttaaaata atccttacct
     1501 ttagtaaagc atgacgtgca gtcggagcat aacatgcggg atgtgctcat gagaacgtcc
     1561 aatagcctgc aggttattat taactaaccc cagacacgtg cggacgaatg aagactgctc
     1621 accttattct tgcgtataaa aatccttatc aggtaaggcg tttggccagg gtgctgaccg
     1681 catgcccgga gtttgattgc tgggtacata tcgataagaa ggtggacata gaactgttcg
     1741 ctcctttcct gcatctcccg cgggtcagat ttatccgaaa cagggttccc gtaaattggg
     1801 gcggctatag ttgcctggag gctgcattta atggcctccg ggagattatc gaaagtaagg
     1861 aagactacga ttttgtaaac ttattgagcg ggcaggacta tcctatcaag cctgtcgatg
     1921 agatcaatga ctttctctcg gccaaccgga ataagtcttt cttggcggtg gaaaccgaac
     1981 catctgcatg gtgggagcac gcgataggcc ggttgaccaa gtatcatttt accgattttg
     2041 gtttcaaggg caaaacggtc ttacaacagt tgatgaaccg gctgatgccc gacaggcagt
     2101 tcatgcaatc attcagtctt tacggcggac cttattcttc gtattggacg cttagcatgc
     2161 aggcggcggc atatgtccac gatttcgtag agaacaataa gaagctggtt gatcattgca
     2221 ggtatacctg ggcgcccgat gagttcctgg tcagtactat cctgatgaac tcgcccctaa
     2281 gagacgaact ggtaaacaaa agcctgaggt atattgattg gtccgaagga gggggtcatc
     2341 cccgcatcct caatgcttcc gatttcggtc cgctgatgaa ttcctcctgc cttttcgcgc
     2401 gaaaatttga catggaagcc gatccaaaat tgttgacaaa agtggatacg cttctgctcg
     2461 gccaaactat tgacacctta tcttaatctt cccctccaca atttgcccag ccatcgtgat
     2521 ccctacgatt gcagccgaat atactgcctg ctggcggccg gtgaagaaat caacttttgc
     2581 cttcccattg ttgtcggtca cgatatcgcc gttccagtat aacgtggacg catcgaccgt
     2641 tccgtcacca ggacctggcc ctttcgccgg atcccagccg gacgcataga catttggccg
     2701 gctgtaatag ccccttggat agatcgtggt aaggccctta gcgttggttt gagcaaccgc
     2761 ctcgctcttg ttaaccgaat tgatcaggat tacgcccccc gaaccctgca tcccgtagac
     2821 ggctgtcagc ggccccttca gcacttcaat gaattcgata tttgaggcgt ccagggattc
     2881 gataatggcc gcattctgcg gaaccccgtc cagcaacacc aaaaccgggt ccgcagttcc
     2941 caggccgaca acacggttaa tggctgaggt tagccccggt acagaggcaa gtgcatcctt
     3001 tatagcatct actccgcctt tgtgtaactg gtcgggtccg atgacatccg aaaaggaact
     3061 cgcccgcttc gcctcatctt caggcggcag tcttgaccgc ccctttacgg tggccggctt
     3121 gagcaatccg gtatccttca actgcgccag atccggcgat gattccctgg aacggtaacg
     3181 gcggatgatg gcgatctcgt cctttccgag gccctgtttg agcaccgccg gcgtgcgaaa
     3241 cgcgggatag tccattttgt cgataatcag tttccccgtc gcggccttac cctgcagggt
     3301 ggtaagcttc atattgaacc ttgtgccgtc gtcgtattcc gtcagcggaa agctgaatct
     3361 gccttgcgta tccgtcgtgt ccgccatgaa aaagcgattg tcgccggata ccaggttgac
     3421 aatatagtgc tgtaacgggg cgttgtcgtt gccgatcgcc ttgccacgga tgttcagcag
     3481 attactgtcc acaaaccggg gcaggttcac cgtcctggtt tcgcccggca gatatttcca
     3541 gccggagtag gagggcgccg ccgtcagcat cagaacgtcc tctcccgtat ctccggcgtt
     3601 tccggcaagg tcggccggag ccgtcccgaa tggagcgact tcgccgagtt ccgcattaag
     3661 actgcgatcg ccggtcagcc ggtcgtcggt tacggccact gccagcaggg tcgcggtttg
     3721 aggatgattg tcctccgcgc ggacgccgat gctcagcccc accttttcac ccggcccgta
     3781 atgcgcttta tccgttgccg cctggagccg cccgctgtcc ctgccgggga cataaacgct
     3841 gcgctgactc accatccggt cccggtcatc gaacaaatag aagctgacct ttcccggcgg
     3901 caatgcggcg acgggaacat taacctggta catgtcggtg ccattggcgg caaaacacaa
     3961 gctgtcgcgg ctgacagcga gcaatttggt agacttgttc ttcttgtaca gggaatcgcc
     4021 gagcgagact tgcaggtgta taatccggtc gtcgcgcccg atgacggaga gctgataggc
     4081 gaactggttg agcgccggta gcgggaaaag cagctcccgg ccgcgccagc tgacatgagc
     4141 ggtgtatttc ctgggattgt aggcatcgaa actgaatttg cccgtccccg gaaggacgct
     4201 gtgaaagcga gctacggtat cgtgcaacgg atcggtcaca taaccggaga cgtcgaccgg
     4261 ctgacccctt gatcccaggg cgcgaaagcc gacggtggcg gtggtgcctg agatgatcga
     4321 tccgccctcc ggatagaaca gaaggctcgg cgcggcggta tccgtgccca ccgggaccgt
     4381 ctttggcgcg taggccgaaa gcgcgccggg gttcggcttg ttgatgttga caacataaac
     4441 aggccgtaca aaaatgcggc tgctgtcttc ggtcaacatc cgcgtggtat aggcccgcag
     4501 ccaatagtag ccttccggca gggtggcggg aagcaggatc atgccggcgg ttttatgggc
     4561 cgggatattg aacaggagtt ggctgatcac ggagtcctga tcattgacca ggtccacata
     4621 caagtttttg ctgagataca ggaagcggtt ggaaagtgaa tcgaggcacc aggcgttgaa
     4681 ccagatcttt tcaccggcgg cataatatgg tttgtccgtc agaaccatca gcttctcctt
     4741 cctgcccgta cggatatgtt ctatgaattg cgcggccagc tcattcatgg aagtctgggc
     4801 gtcggcggca gtgaccagca acagacagcc ggcgagcagc aggttttgat aatggcgtaa
     4861 catgatgggc gctttgttga taagcccctt aaggtacaca atttatttca ccgcttcgca
     4921 attggccttg aatacgcgga taaaattgcc gcccaggatc ttttccacat cggacggctt
     4981 atatccccgt ttcagtaatt cgcgtgtgat caacggataa caggtgacgt cgttcaactg
     5041 ctgtggcggc gattcgatcc cgtcgaaatc cgatccgagg ccgacatgat cgaccccgat
     5101 cagccggacg atataatcga tatgatccat gagcagcgac agcggtgcac gcagtgaatc
     5161 actttcagcc ggatatttcc tgctcaggaa ttcgttgatg gcatactgcg gcgttttggc
     5221 agcgttcata ctgtcgagct ccgccttgtg gcgcccgagg aatcgcgtta gccggggcgc
     5281 gtaattgctg tccaggaaac cggaatagaa attcacctgg atgacgccgc cgttgccgcc
     5341 gatggccttg atctggtcgt ctttcaggtt gcggaacaca gggcatagcg cgtaaacgct
     5401 gctgtgactg cagatcaccg gtttggttgt cgtatggatc gcatcccaga acgttttttc
     5461 gccgacgtgg gacaggtcta ccatcattcc cagctcgttc atgtgccgga caatggccct
     5521 gccttccgcc gtaagcccat agggcgttac ggaaaacgcc ctgcccgttt catccatcgc
     5581 cgaggtcgcc cagggcgtgc tgttgttcca cgtaagcgtc atatatcgca cgccgagccg
     5641 gtaaaaactg tcgagatagt ccatccggtc ctcgatcata tgcccgcctt ccacgcccat
     5701 catggctgcc agtttgtggt gtcgaacagc cgactccagt cctgcgggat cggtgacgat
     5761 ctccatcttg tccgggttcc ggcgcgcgat cgcatacagg ctgtcgattt cgcgatgggc
     5821 catgtgaaag gcgctgccga cgccataatg gtcatcgcaa aaaatggaaa atacctggac
     5881 atccactccg ccttttttga agcgcgccag gtcggagtgc gttttcccgg tcagatccga
     5941 tccgatatcc attccattca tcgttgcggt cgacagcacg tcgttgtgcg tatcggctac
     6001 gatggctttg tcgtgcagtt tgagataggc ttgcccgtcc gcgtgaatgg cgggcgcgaa
     6061 gcagccgagg gcaagggcca atgaaaatag ctgaagcttc atgtggagcg gtttattttt
     6121 cgcggtgaaa atccaagtcc tggaaatcat aactgaagtc cgtaagcggc gaaaccggtt
     6181 tcattcgaat ttcggttgcg attccgcgct cgtcgagacc gaagctgaca tatgcgtcgg
     6241 cgtccatact ccggtcgacc catttgacga taaatgaatt ccctttgaac ggcagcattt
     6301 cgccggtcaa cctgggcgaa cgcctgctgt caaaccatag cctgccatat ttcaccgata
     6361 tggtcacatc gccgagccag ttgtcgcggt aatccccggt atacgccgac cagtcggcgc
     6421 cccgcgctcc ggcgcccttc tgcaccgcct ccacctcttt ccaaatggaa tccgtgatcc
     6481 gctttgcgtg ctcgagttgc gcttttcggc cggccgcgta ttcacttacc ctgtccgtgc
     6541 ccgtgatacc caggtagctg tccttgatct ggttggtgat
//
LOCUS       DATGKE010000238         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1472902, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000238 DATGKE010000000
VERSION     DATGKE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472902"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1218)
                     /locus_tag="VKU83_04125"
     CDS             complement(199..1218)
                     /locus_tag="VKU83_04125"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HLI92765.1"
                     /translation="MLHRMHIYYHTDELPVFRKSVVTIGSFDGVHTGHAQILGQLRAE
                     ADRIGGETVIITFYPHPRRVVGGGLGQVKLLNTLAEKIQLLSWRQIDHLVVVPFTESF
                     SRLTAQEYVHEFLVAKFHPHTVIIGYDHRFGKGRHGDYHLLEQFSRSEGFQLEEIPVQ
                     LLDAVSVSSTRIREAIGQSDIASANKLLGYPYFFTGQVVEGRRLGKSIGFPTANLRIN
                     DPEKLIPANGVYAVEVSVLESGDISLTAPTTVVAPRTAPWAGPGYQGMMNIGVRPTVD
                     GREKTVEVNVFDLDQDLYGRELRVSVRQWLRPEQKFSGLEELRQQLGRDKQDAITALQ
                     ALPIQ"
     gene            1255..>2372
                     /locus_tag="VKU83_04130"
     CDS             1255..>2372
                     /locus_tag="VKU83_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HLI92766.1"
                     /translation="MSLYAQRGVSAQKEEVHQATEKLEKGLYPNAFCKIFPDFLGGDK
                     DWVDVMHADGAGTKSILAYLYWKETGDLSVWDGIARDAIAMNLDDLLCVGICDNLLFS
                     STIDRNKRLIPGEVLSAIINGSQAFFDELGQHGVRIRYLGGETADVGDVVRTIAVNGT
                     MAARWPKDKLVTNDRIGAGDVIVGLASFGCAKYETAYNSGIGSNGLTSARHDMLEKAY
                     AARYPESLDNGLNDNVIYIGPHRLTDPVEVSGHGTVDVGHLLLSPTRTYAPVLRCLLT
                     EHFIDIHGLIHCSGGGQTKCLKYLPEGLLVVKDNLFDPPPVFQLIRQASGSDAREMYQ
                     VFNMGHRLEIFTTPDAAERMIRISNSFGIDARIVGRVES"
BASE COUNT          528 a          684 c          655 g          505 t
ORIGIN      
        1 cgggcttctg ctgatgcacc attataacct gaaaagcctc ggtgtcggaa gcgccggcgt
       61 tgaagatgca tcgctcctga aagagctggt gatcaaagta atgagcagct gaaaggcaag
      121 cgaagggccg catgcgtccg ccgtccgggg gcaaccacaa agatcaggtt cagaaatttc
      181 cgcttcccgg accggaaatc attggattgg cagggcctga agcgccgtga tcgcatcctg
      241 cttatccctt cccagctgct gcctgagttc ctccagcccg ctgaatttct gttccggcct
      301 taaccactgc cggaccgata cccttagctc tcttccataa agatcctggt ccagatcaaa
      361 aacattcact tcgaccgtct tctcccggcc gtccacggtc ggccgcacgc cgatattcat
      421 cattccctgg tagcccgggc cggcccaggg tgcggttcgt ggcgctacca ccgtcgtcgg
      481 cgcagtcagg gagatgtcgc cgctttccag aaccgacacc tccaccgcat acacaccgtt
      541 ggcgggtatc agtttctcgg ggtcgttgat ccggaggttg gcagttggaa atccgatcga
      601 tttccccagc cgcctgccct cgacaacctg gccggtaaag aaataagggt atcccaatag
      661 cttgttcgcc gaggcgatat cggactggcc gatggcttca cggatccgcg tggagctgac
      721 ggaaaccgcg tccagcagtt ggacggggat ctcttccagc tggaaacctt cggacctgct
      781 gaattgctcc agcagatggt agtctccatg ccggccctta ccaaaccgat ggtcatagcc
      841 aatgatgacc gtatgtggat ggaatttagc caccaggaat tcatggacat attcctgcgc
      901 cgtcagcctg gaaaagctct ctgtgaaggg tacgacaaca aggtgatcaa tttgtcgcca
      961 ggaaaggagc tggatctttt cagccagggt gttaagcagc tttacctgcc ctaagccgcc
     1021 cccgaccacc cttcgtggat ggggataaaa agtgatgata accgtttcac ccccaattcg
     1081 atctgcctcc gcgcgcagct ggccgaggat ctgcgcatgc cccgtgtgaa ccccatcaaa
     1141 actgccgatg gtaaccactg atttacgaaa gaccggaagc tcatcggtat gataatagat
     1201 gtgcattctg tgcagcaaat ctaacggaat tattgtacta tcttcgcgcc cattatgtca
     1261 ctctacgcgc aacgtggcgt ctctgcccaa aaagaggaag ttcaccaggc gacggagaaa
     1321 ttggaaaagg ggctatatcc aaatgctttc tgtaaaatct tcccggactt cctcggcggt
     1381 gacaaggact gggtcgacgt catgcacgcg gatggcgccg gcaccaagag catcctggct
     1441 tatttatatt ggaaagagac cggagacctg tcggtctggg acggaattgc ccgggacgcg
     1501 atcgcaatga acctcgacga tcttttatgc gtcggcattt gcgacaacct gctgttttca
     1561 tcgaccattg accggaacaa acggctgatc cccggggaag tactgtcggc aatcattaac
     1621 ggcagccagg cattttttga tgagctggga cagcacggcg tcaggatccg gtaccttggg
     1681 ggagagacgg ctgatgtagg ggacgtcgtg cgcaccatcg ccgtcaacgg aacgatggcc
     1741 gcccgctggc caaaggacaa actggtcacc aatgaccgga ttggcgcggg cgacgttatc
     1801 gtcggcctgg ccagctttgg gtgcgccaaa tatgaaaccg cgtataacag cgggatcgga
     1861 agcaacgggc tgacctccgc ccgccacgat atgctcgaaa aggcctacgc tgcaaggtac
     1921 ccggaatccc tcgataacgg actgaacgac aatgtaatat atatcggccc ccaccgtctt
     1981 accgatccgg tggaagtctc cggtcatggg actgtagatg ttggccacct actgctgagc
     2041 cccacccgca cctacgcccc ggtccttcga tgtcttttga ctgagcattt tattgatatt
     2101 catgggctta tacattgcag cgggggtggt cagacgaagt gcctgaagta ccttcccgaa
     2161 gggctcctgg tcgtcaaaga caaccttttt gacccgccgc cggttttcca gctgatccgc
     2221 caggccagtg gcagcgacgc ccgcgagatg taccaggtat ttaatatggg gcaccggctg
     2281 gagatcttta caaccccgga cgctgccgag cggatgatcc ggatcagcaa ctccttcggc
     2341 atcgacgcgc ggatcgtcgg ccgcgtggag tc
//
LOCUS       DATGKE010000239         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1368827, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000239 DATGKE010000000
VERSION     DATGKE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368827"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VKU83_04135"
     CDS             <1..191
                     /locus_tag="VKU83_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92767.1"
                     /translation="SLFEIKKETRLASIPVIVYTTSNDVGESVRLKQLGAAHFMSKPV
                     LPDDVYYMVSYVLGENWN"
     gene            complement(220..597)
                     /locus_tag="VKU83_04140"
     CDS             complement(220..597)
                     /locus_tag="VKU83_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI92768.1"
                     /translation="MDDKKSVLIEKIKTLIIELVHYSEEPLATNLSTYLAETLNHDYT
                     YLANLFSEVQGTTIEKFFISHKIERVKELLVYNELTLTEIAYKLHYSSVAHLCTQFKK
                     VTGLTPSHFKSLKEKRRSMLEDL"
     gene            complement(996..1289)
                     /locus_tag="VKU83_04145"
     CDS             complement(996..1289)
                     /locus_tag="VKU83_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92769.1"
                     /translation="MLQLFFLFISTLVMIFFIAVLTRAFNQWKNPISIGLYVKGLRAE
                     NDGHFEEAITAYETALAQCKKLRFQQVFKNKIIGKVKLLHTLVDYDNSARYIR"
     gene            complement(1351..1833)
                     /locus_tag="VKU83_04150"
     CDS             complement(1351..1833)
                     /locus_tag="VKU83_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92770.1"
                     /translation="MKKALYLIPVSMIMACASVQAQQAQKANIDSLELVVQISQDQLK
                     LGKLQNMIDQRNTNKENAVVDAQNSASDNVRAAERLDADPNNKKLARDANNKAGDAKS
                     DAGKSRKQSRRLDDLNKEIMDLKTKIALAQAKLSIYYPTPQPTPLTMPAPQNDSTQHP
                     "
     gene            2281..3363
                     /locus_tag="VKU83_04155"
     CDS             2281..3363
                     /locus_tag="VKU83_04155"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLI92771.1"
                     /translation="MPKITFDNRNNTFFQSVKRSVDTYFHTQQLKRTGNWKLYLKAWI
                     LIPAALGMYVFLLFGHYSWLPGVLGSVVFGLLLVCIAFNVMHDACHNSFSEKKWVNKL
                     MGLSMNALGSNAFIWKIKHNVIHHTYTNVDGIDDDIANGPLLRQCTSQKWMPIHRFQF
                     LYMFVLYGISTLSWALGTDFARYFRKKIHHTPINRISVKEHVVFWASKLLYAFFYALL
                     PIYILGWQTWLIGFLVINITMGLSLSVVFQLAHIVEKTTFEVASGTQKVIDSEWAIHE
                     IRTTSDFAPENKIISWLVGGLNFQVEHHLFPQISHIHYQALSKIVRQQCEVFGLPYNY
                     YPSARQALYSHIRLMKRLGSIPRSNP"
BASE COUNT          845 a          765 c          818 g          983 t
ORIGIN      
        1 aaagcctgtt tgagataaaa aaggagacgc ggctggcgtc cattcccgtg atcgtttata
       61 cgacctccaa tgatgtcggg gaatcggtga gattgaagca gctgggtgcg gcgcatttta
      121 tgagcaagcc ggttcttccc gacgacgtat actatatggt ttcatacgtg ctgggagaaa
      181 attggaatta gcgcgcagcc gcggattcgc tttattctgt cagaggtcct ccaacatcga
      241 acgccgcttt tccttcagcg atttgaaatg agacggcgtc agtccggtca ctttcttgaa
      301 ctgggtacaa agatgcgcca cgctgctgta gtgaagcttg tacgcgatct cggtcagcgt
      361 tagctcgtta tagacaagga gctcttttac gcgttcgatt ttatgggaaa taaagaactt
      421 ttcgatggta gtgccctgca cttccgaaaa aaggttagcg agataggtgt aatcgtggtt
      481 taatgtttct gctaaatacg tcgacagatt ggtggctaat ggttcctcgg aatagtgcac
      541 caactcgata attagggttt ttattttctc gatcaagacg ctttttttgt catccatcaa
      601 ctcaagcccg tactttaaca gcgttgcccg caacagttca cgctggtccg gggaacggtt
      661 ccgacgattt ccgcctctcc taattcaaca gaagtgtgcg gcagcccgag ttttgtcaac
      721 tcatctttaa cgaccatttt gcagcggata cataccatat ttctgatata gagcttcaag
      781 attttgattg ttacgtgatt ggagcaatag aaaaatctgg tgagcgccaa cattaaagat
      841 accgaatttt tggctccccg gcagggcctg cctttgcgct cgcgacctcg ccggttactg
      901 gctagacaaa acgagcggca agtttgccat ggtcgtgcga aacgacatgg accggtcttg
      961 tttggtcatt gataaccagg gattgggaag ggggattatc gaatgtaccg ggcactatta
     1021 tcgtaatcaa caagcgtatg caatagcttc actttcccga taattttatt tttgaagact
     1081 tgctgaaacc tgagcttttt gcattgggca agcgccgttt cataggcggt gatagcctct
     1141 tcaaaatgtc cgtcattttc ggcccttaat ccttttacat ataatccaat ggatatagga
     1201 ttcttccatt gattgaaggc gcgcgtcaga acagcgatga aaaaaatcat gacgagcgtg
     1261 gaaataaata aaaaaaatag ctgtaacata aatcataaat taaaaagaga aaagaaagcg
     1321 gactttggat gcccgcctca tccagctaga tcacggatgc tgggtgctgt cgttctgtgg
     1381 tgccggcatc gtgaggggcg taggttgcgg cgtcggataa tagatactca gtttagcttg
     1441 tgcgagcgct atttttgttt ttagatccat tatctcttta ttcaggtcat ctaaccggcg
     1501 ggattgcttc cgcgactttc ccgcatcact tttcgcatcc cctgctttat tattggcatc
     1561 acgggccaat ttcttgttgt tgggatctgc gtccagcctt tctgctgccc taacgttatc
     1621 gcttgctgaa ttctgggcat cgaccaccgc attttccttg ttggtgtttc gttgatcgat
     1681 catgttctgc agcttcccga gcttaagctg atcctggctg atttgaacaa cgagctcgag
     1741 tgagtcgatg ttagcttttt gtgcctgttg tgcctgaacc gaagcgcatg ccattatcat
     1801 actgactgga attaaataca aagctttttt catgtttttg tttttaacga ttgaaattgc
     1861 gttcctaacc gcagaatcgg cctcaataca ggtctaaaga aattgttcgc gaacaattgg
     1921 ctgaagggat gcagaagaat aattgagcga gatagtcgat gtttcagagg taccggtaaa
     1981 ggtactgcat ctcaaaggga acatgttata tcatccatag cggcgcttac ataattcaca
     2041 cgttcccgca ttgaggcgct taatccccaa aggactattg cacgaaagta atatatgcta
     2101 tataattaac atattcaaaa cttagccggc cggaatatgg gaattatgta acgggggcag
     2161 ggttttttat aacagctgct ctcgttggaa tccttacttt cggctaggtt tttcacagga
     2221 ttttggagaa taagggggcg ggatccgtat cccgcccttt tttcatttta aatctggctt
     2281 atgcctaaaa taacgttcga caaccgcaat aatacgttct ttcagtctgt caaaaggtcc
     2341 gtggatacat atttccatac gcaacaattg aaaagaaccg ggaattggaa actgtatctg
     2401 aaagcctgga tactcatccc ggccgccctt ggcatgtatg ttttcctgct gttcggtcat
     2461 tacagctggt tgccgggtgt tctcgggtcg gtggtctttg gattgctttt ggtttgcatc
     2521 gctttcaatg tgatgcacga cgcctgccat aacagcttct ctgagaagaa atgggtaaac
     2581 aagctgatgg ggttgtctat gaatgcactg gggagcaacg cctttatatg gaagatcaaa
     2641 cacaacgtta ttcatcatac gtataccaat gtggacggga tcgacgatga tattgccaat
     2701 ggcccgttat tgaggcagtg cacctcgcaa aaatggatgc ccatccatcg atttcaattc
     2761 ctctatatgt ttgtgcttta tgggataagt acgctgtcct gggccctggg aaccgatttt
     2821 gcgcggtact ttagaaaaaa gatccaccat acgccgatca accggattag cgtcaaagag
     2881 cacgttgtat tttgggccag taagttgctt tacgccttct tttatgccct gctgccgatt
     2941 tacatcctgg gttggcagac ctggctgatc ggttttctgg ttatcaatat aaccatgggc
     3001 ctttccctgt cggtagtttt tcaattggca catatcgttg aaaagaccac gtttgaggtg
     3061 gcctccggga cacaaaaggt aatagattcg gagtgggcca tccatgagat tagaactact
     3121 tccgacttcg ctccggaaaa taagatcatt tcctggttgg tgggcggcct gaatttccag
     3181 gtggagcatc acctcttccc gcagatcagt catatccact accaggccct gagcaagatc
     3241 gtgcgacagc agtgcgaggt attcggcctt ccttataatt attatccctc cgcacggcag
     3301 gctctttact cgcatatccg gttgatgaaa agattgggaa gcataccgcg gtcgaaccca
     3361 taaaagcgac aagcattcta ccggccggcc ggccgctata ccttataaac c
//
LOCUS       DATGKE010000240         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1344652, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000240 DATGKE010000000
VERSION     DATGKE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344652"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VKU83_04160"
     CDS             complement(<1..441)
                     /locus_tag="VKU83_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92772.1"
                     /translation="MKTTLLYRFLFVIAFTGALSSCNKSNSSNNSTSNADLQTNSDDA
                     TRVSTETDAAFNDVNSAMTAQYSVTGAATGREMQQRVLDGPGGSVDSGLICDGTITMD
                     TASNPRTITITYNGNNCSLNRTRKGNIVISWPAGQLWRDSGAVVT"
     gene            608..1195
                     /locus_tag="VKU83_04165"
     CDS             608..1195
                     /locus_tag="VKU83_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI92773.1"
                     /translation="MYLSGMDQYRLPPQTRIGHVHLKVADLQRALDFYCELLGFELIM
                     RYGADAAFVSAGGYHHHIGLNTWYSKGQPPAPGSSPGLFHTAIVYPGRRELAIALKRL
                     LDAGYPLTGASDHGVSEAIYLDDPDGNGVELYWDRPRENWPRDEEGKLTMYTRRLDLS
                     GLLKEIGSRKGQPDAQGRQEGPGAAASSTGWPPGD"
     gene            1332..>2280
                     /locus_tag="VKU83_04170"
     CDS             1332..>2280
                     /locus_tag="VKU83_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HLI92774.1"
                     /translation="MRFPSNAKIALQRKIAWLLFFSFFSLCLVGWVIYFNISDRGLAY
                     LLIGANILTFLFIFVILVRLNHDIMLRKNAEGRLKENQAWLQSILDNSTSLMYIKDLG
                     GRYVMVNRRFREMLDLGDTGIIGKTDHEFSDADAADHYKMLDQEVMRTGKSLEIEEVI
                     EHEEKKVHLLSMKFPLLDNNGRQIGIGGIATDITERVNYQQQLISATKEAHKAKEMQE
                     LFLANMSHEIRTPMNGIQGMTDLLLDTRLSEQQMEFARIIKRSVNNLLVIINDILDFS
                     KIKAGKLTIEKIDFRLRDVLDNVLGIFDHRVKKKGLKLSI"
BASE COUNT          571 a          544 c          638 g          527 t
ORIGIN      
        1 cgtaacgacg gcgcccgaat cccgccaaag ctggcctgcg ggccaggaga tgacgatatt
       61 ccccttgcgg gtgcgattca acgagcaatt attgccgtta taggtaatgg tgatggtgcg
      121 gggattggaa gcagtatcca tggtaatcgt accgtcgcag atgagtcccg agtcgacgga
      181 gccgccaggg ccatccaaga cccgctgttg catctcacgg ccggtagcgg cgccggtaac
      241 tgagtactgc gcggtcatgg ccgaattgac atcgttaaag gcggcatcgg tctcggtgct
      301 aacccgggtt gcgtcgtccg agttggtttg aagatccgca ttggatgtgg aattgttgct
      361 ggaattactt ttgttacagg aagaaagggc gccggtgaag gcaatgacaa agagaaagcg
      421 gtaaagcaat gtggttttca taaagatctt gattttagct tttttttgtt ttcaataata
      481 cggttcgtct aagaccgtgc agggacgcca tggtttaacg gtatttggaa tattattatt
      541 ttttgtctgt ataaaagaaa cgggatgatc gggcgtcatt aacaaaaaac cccagaaacc
      601 cccagaaatg taccttagcg ggatggatca atacaggctt ccgccccaaa caaggatcgg
      661 ccatgtgcac ctgaaggtag cggacctcca gcgggcgctc gacttctact gtgaactact
      721 tggatttgaa ctcattatgc gctatggtgc cgatgccgca tttgtctccg ccggaggcta
      781 tcaccaccat attggtctca atacgtggta tagcaagggt cagccgccgg ccccgggttc
      841 ttcgcccggg ctgttccata ccgcgatcgt ctatcccggg agacgagaac ttgctatcgc
      901 gctaaaacgg ctgttggatg ccggttatcc cttaacagga gcttcagatc acggcgtttc
      961 ggaggccatc tacctcgatg atccggatgg aaacggcgtg gaactttact gggaccggcc
     1021 gagggaaaac tggccgcggg atgaagaagg caagttgacg atgtacacgc ggcggttgga
     1081 tctttccggc ctgctgaagg aaatcggctc gcggaaaggt cagccggatg cgcagggacg
     1141 acaagaagga ccgggagccg cagcaagctc aaccggttgg cccccgggtg attaagagaa
     1201 cgggtaagtg tattcactta ttactgatta aaatcggcag gaaataacag ggagggaaaa
     1261 tcttattcgg ctttctgcgt tatatgaggc agaccgctgg acacagtccg gaccctaaga
     1321 ccgtaatctc tatgcgattt ccttcaaatg cgaagattgc gcttcagcgc aagatagcat
     1381 ggctcctttt cttctctttc ttctcgcttt gcctggtagg atgggttatt tattttaata
     1441 tttccgatcg gggcctagcc tatctgctga tcggcgccaa catcctcact ttccttttta
     1501 tttttgtcat tctcgtccgg ctgaaccacg acataatgct tcgaaaaaac gcggagggcc
     1561 ggttgaagga aaaccaggcc tggcttcagt cgatcctgga taacagtact tcactgatgt
     1621 atatcaagga cctcgggggc cgctatgtca tggtcaacag gcggttccgc gagatgctgg
     1681 atctgggtga taccgggatc attggcaaga ccgaccatga attcagtgat gccgacgccg
     1741 cagaccatta caagatgctc gatcaggagg tgatgcgcac cggtaaatcg ctggagatcg
     1801 aggaagtgat agagcacgag gagaagaagg tgcatttgct atcgatgaaa ttcccgctgc
     1861 tcgataacaa cggccggcag attggcatcg gcggcattgc cacagacatc acggagaggg
     1921 tcaactacca gcagcaactg atcagcgcta cgaaggaagc gcacaaggcg aaggaaatgc
     1981 aggagttgtt ccttgccaat atgagccatg agatccggac gccgatgaat gggatccagg
     2041 gaatgacgga cctgctgctg gacaccaggc tatccgaaca gcaaatggaa tttgcccgga
     2101 tcatcaagcg gtcagtcaat aacctgcttg tcatcatcaa tgatatcctc gacttttcca
     2161 agatcaaggc gggcaagctg accatcgaga agatcgattt ccggctcagg gacgtcctcg
     2221 acaatgtatt gggtattttt gatcaccggg taaagaaaaa aggactaaaa ctttcaataa
//
LOCUS       DATGKE010000241         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1539797, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000241 DATGKE010000000
VERSION     DATGKE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539797"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VKU83_04175"
     CDS             <1..1776
                     /locus_tag="VKU83_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLI92775.1"
                     /translation="LGILFARPGKGIMRQVTGPYTGSVMSRRLLPFALLVPAAFGWAA
                     LFSPWRNTISIQLSAAVLVTAIILISMAFISYNTVLLNRRDLAAKQAHHELQAIEQRF
                     SMLINSVKDYAIVMIDHAGRVLSWNNGAQAITGYTAEEAIGRNVSLFYHHRDIEDGVP
                     EYNRRQAKVNGQFHREGWRPRKDGSRYWAEVTWTAIWDDQRRLQAYIVIVRDTTERKR
                     AQEKIAYQARLMEDSSDAVFSVDPDYRLVSFNKAAEALYGYTAAEVIGKPLNDVLRNP
                     MDDATRAAIRRELLEVGYWRGDVVYHTRDKGVLDISISISRTSDERGRVDGYIMVCRD
                     MTERLKAEARLRKFNEELERQVKEKTAALVRSNTELRDLSTRLQLIREEERAVMAREI
                     HDELGQQLTGLKMDLYWVARKLDGAAPDQAREKLRSTISLLDNTIQTVRRIATDLRPS
                     ILDDLGLIAAIEWQSQEFQKRSGIRTTFTSSVAEMDFPPEMSIGMFRICQESLTNVAR
                     HASASNVSITLDQAGGEVRLVIADDGKGLDPERADNGKTLGLLGMKERALMMGGRLEF
                     GNGEKNGLQLVLTVPLAGAPSHVLT"
     gene            1794..2429
                     /locus_tag="VKU83_04180"
     CDS             1794..2429
                     /locus_tag="VKU83_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI92776.1"
                     /translation="MLRILIADDHTVVRKGLRQILLEEFPSAEIVETPDAASLFLKVL
                     REEWDVVITDISMPDKSGLEALQQIRRDYPRLPVLVLSAHSEDQYAIRSLKAGASGYL
                     CKDSASEELVGAVRRILMGKRYITASLAEKLASQLDQDADRLPHENLSDREFEVMRFL
                     AAGKAVSEIAGQLCLSVTTISTYRARILTKMDLKTNYDLTRYAMENKLLEQ"
BASE COUNT          604 a          815 c          787 g          542 t
ORIGIN      
        1 ctcggcatcc ttttcgccag acccggaaaa gggatcatgc ggcaggttac gggcccttat
       61 accggaagtg tcatgtcccg ccggctcctt cccttcgcgc tgctggttcc agccgctttt
      121 ggctgggcgg ccctctttag cccatggcgc aacacgatca gcatccagct gtcggccgcg
      181 gttctcgtga ccgccattat cctgatctcg atggccttta tcagctacaa taccgtttta
      241 ctgaaccgcc gggacctcgc tgccaaacag gcccaccatg aactgcaggc catcgagcaa
      301 cgatttagca tgctcatcaa cagcgtaaag gactacgcca ttgtcatgat cgaccacgca
      361 ggacgcgtcc tcagttggaa caatggggcg caggcgatca ccggctacac ggcagaggag
      421 gccattggac ggaacgtgtc cctgttctat caccaccgtg acattgaaga cggtgtcccg
      481 gaatacaacc gaaggcaggc caaggtgaat ggccagttcc atcgtgaagg atggcgaccc
      541 cggaaggacg ggtcccgtta ttgggccgag gttacctgga ccgccatttg ggacgaccag
      601 cggcggcttc aggcatatat tgtgatcgtc cgcgatacga ccgagcgaaa gcgggcccag
      661 gagaagatcg cctatcaggc gaggttaatg gaggactcga gcgacgcggt tttctcggtc
      721 gatcccgatt atcgcctcgt cagtttcaat aaggccgccg aggcgctgta tggctataca
      781 gcggccgaag tgatcggcaa accgctcaac gatgtgctca gaaatccgat ggacgatgca
      841 acccgggcag cgatccgccg cgagttactc gaggtcggct actggagagg ggatgtcgta
      901 tatcataccc gggataaggg tgtcctggat atctctattt cgatctcccg gacgagcgac
      961 gagcggggta gggtggacgg gtatatcatg gtctgccgcg acatgaccga aaggctcaag
     1021 gccgaagccc gcttgcgcaa attcaacgaa gagctcgaga gacaggtaaa ggaaaagacg
     1081 gccgcgcttg tccgctcgaa caccgaactg cgcgacctca gcaccaggct ccagctcatc
     1141 cgcgaagagg agcgggcagt tatggcccgg gagatccatg atgagctcgg ccagcagttg
     1201 accgggctca aaatggacct ttattgggtt gcccggaaac tggacggcgc agcccccgac
     1261 caggcgcggg aaaaactgcg cagcacgatc agcctgctgg acaatacgat ccaaaccgta
     1321 cgccggatcg ccaccgatct acggccaagc atcctcgacg acctcggcct gatcgccgcc
     1381 atagaatggc aaagccagga gttccaaaag cgctcgggca ttcgcaccac ctttacgtcc
     1441 tcggtagccg aaatggattt ccctcctgag atgtccatcg ggatgttccg gatttgccag
     1501 gagtcgctga ccaacgttgc aaggcacgca tcggcgtcta atgtgagtat cacgctcgac
     1561 caggccggag gtgaggtacg cctcgtcatc gccgacgacg gaaagggcct cgaccctgaa
     1621 agggcggata atggaaagac ccttggcctg ctgggcatga aggaaagggc cctgatgatg
     1681 ggcggccgcc tggagttcgg aaatggagaa aaaaatgggc tgcaactggt cctgaccgta
     1741 cctttggccg gtgcgccatc gcacgtttta acctaaataa ccgctattcg cgcatgctaa
     1801 ggatacttat cgctgacgac cacacggtgg tcagaaaagg cctccgccag atcctgctgg
     1861 aagagtttcc cagcgcggag atcgtcgaga ccccagatgc cgcttcgcta ttcctgaagg
     1921 tcctccggga ggagtgggat gtggtgatca cggacatttc catgcccgat aaaagcgggc
     1981 tggaagccct gcagcagatc cgtcgtgatt acccccgcct gccggtcctt gttctgagcg
     2041 cccattcaga ggaccaatac gccatacggt ccttaaaggc cggcgcctcc gggtatctct
     2101 gcaaagactc tgcttccgag gagctggttg gagctgttcg ccgtatcctc atggggaagc
     2161 gctatatcac ggcgtccctg gcggaaaagc tcgcttccca gctcgaccag gatgccgacc
     2221 ggcttccgca cgagaacctg tccgaccggg agtttgaggt catgaggttc cttgccgcag
     2281 gcaaggctgt ttccgagatc gccggccagc tgtgcctgag cgtgaccacc atcagcactt
     2341 accgcgcccg catcctcacg aaaatggatc tcaagaccaa ttatgacctt acgcgctacg
     2401 caatggagaa taaattgctg gagcagtaaa gggccaaaag cgataagccg ccccgcccgc
     2461 acttgcgcct tacgatacca gccgtcgatg tagcggatgt actacaccgt accccttcat
     2521 ctttctactt caatagtatt gcctgtgcta cgggggctgt ccgttttcgg ttgtagtttc
     2581 acgctagtgg caccctccgc ctcagaactg aagacgctcc tcatcgtcga tgattccccg
     2641 atcatcatcg accgcttgcg caatatgctg gaaggaatcc tcctgccgga gcaggtccgc
     2701 catgccggca gctacgtcga agccctttcc tttcttctcg taaaggtt
//
LOCUS       DATGKE010000242         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_973016, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000242 DATGKE010000000
VERSION     DATGKE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973016"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VKU83_04185"
     CDS             <1..761
                     /locus_tag="VKU83_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLI92777.1"
                     /translation="MDSVYLYNKYGEPISYDFWKDIEKQMDWLAHNWNQPDDGIWEVR
                     GGKQKFLYSRLACWVALDRAMKVSKMRSFPRNGIWKKERDKIYNSIFTDFWDEKQAAF
                     MQYPGAETVDASSLLMPLFRFISPKDPRWLSSLRRIEKDLVTDSLVYRYRPEVAAPDG
                     FTSREGTFSMCTFWYVECLSRAGLLEKARFYFEKMLGYANHLGLYSEQLGFEGEHLGN
                     FPQAFTHLGLISAACNLDEQFNDNRNKNINLEEL"
     gene            769..2049
                     /locus_tag="VKU83_04190"
     CDS             769..2049
                     /locus_tag="VKU83_04190"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HLI92778.1"
                     /translation="MPVSQRKKPVKSGLRKVLKTMGPGLVTGASDDDPSGIVTYTQAG
                     AQFGFATLWTALITFPLMASVQGMAARIGMVTSQGLTGTLKMHYPKAVLYLMIVSCFP
                     AVVLNIGADIQSMGAVAHLLAPTVPTFTFCILFTAILLFVIIRYSYRRVAALLKWLCL
                     ALLLYLIIPFLVHPDWGAVARRTFVPSIRLDKGFMSILVAILGTTISPYLFFWQATME
                     AEDMEHASRKRIFVDKRMLGDMRRDVNTGMLFSNVVTYFIILTAGVVLFGAGVRQIDT
                     VEQAAHSLRPLAGQLSYLLFAIGVIGTGFLAIPVLAGSVSYMLAETFNFSKGLDKKFY
                     EARQFYLVIIVSLVAGLCLQLFGITPVQALIYTAILYGLTAPVMIVLLMHIGNNKKIM
                     GRHTNSPLSNILGALTLLLMTAAAVGLIWLQLTS"
     gene            complement(2061..2411)
                     /locus_tag="VKU83_04195"
     CDS             complement(2061..2411)
                     /locus_tag="VKU83_04195"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HLI92779.1"
                     /translation="MKQAKNHHRKQKGARGNKSRWSGKVTRNSNALDLDKGVFTLEDP
                     GKIAQSLNRSAHRSTRKKAGSYQSAMSMLNFYINRAGKNLPAKRKKVLTAAKEKLRGL
                     HEREREKQPHHQQR"
     gene            2499..2846
                     /locus_tag="VKU83_04200"
     CDS             2499..2846
                     /locus_tag="VKU83_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92780.1"
                     /translation="MGSTAAMSTISQVLESLRRKNMDLEFRWTPEGFTAGGAKYYQPE
                     ELEIVRVFRFEGESDPSDMAVIYVLRAADGVTGYSLDAYGAYSSHDLESGYNNFIRRI
                     PESDHDQQILFEL"
     gene            2876..>4536
                     /gene="glgX"
                     /locus_tag="VKU83_04205"
     CDS             2876..>4536
                     /gene="glgX"
                     /locus_tag="VKU83_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HLI92781.1"
                     /translation="MTMYPGDPYPLGSFWDGRGVNFALYSHNAEGVELCLFDKPQGEK
                     ETARFKLTQRTHDIWHGYLYDCKPGQLYGYRVYGPYDPSNGHRFNPAKLLLDPYSKAI
                     CTFEKWDDTLFGYEMGKDDLTCDTRDNAMVAPKSVVIDHQFDWEGDRPPKIAYHNTII
                     YEAHVKGLTKLFPGIPSNIRGTYAALAHPMVIDYLKNLGITAIELMPVHQSIPERHLV
                     EQGLTNYWGYNSVGFFAPGMRFYSGKEVGGQVREFKTMVKELHKAGIEVFLDVVYNHT
                     AEGNQLGPTLAYRGIDNNEYYRLAEDKRFYYDYTGTGNTLNTRLPNVLGLIMDSLRYW
                     VLDMHVDGFRFDLAAALARELQEVNRLSAFFSIIYQDPVISQVKMIAEPWDVGEGGYQ
                     IGQFPPGWAEWNGKYRDCMRDYWRGADSMLAEFAERFTGSSDLYKNDNRNPTASINFI
                     TAHDGFTLQDLVSYNERHNEANKEDNRDGESNNRSWNCGAEGPTEDQGIIALRKKQKR
                     NFLATLMLSQGVPMVVAGDELGHSQQGNNNAYCQDNEISWINWKDG"
BASE COUNT          985 a         1197 c         1306 g         1048 t
ORIGIN      
        1 tgatggattc ggtatatctt tataacaaat acggtgagcc catttcctac gatttctgga
       61 aagatatcga gaagcagatg gattggctcg cccacaattg gaaccagcct gacgacggca
      121 tctgggaagt aaggggcggc aagcagaaat tcctctattc gcggcttgcg tgctgggtag
      181 cgctcgaccg cgcgatgaaa gtatcgaaga tgcgttcttt cccgcggaat gggatctgga
      241 agaaggagcg ggataagatt tacaacagca tttttactga tttttgggat gaaaaacagg
      301 cggccttcat gcaatatccg ggtgcggaga cggtcgatgc gtcaagcctc ctgatgccgc
      361 tattccggtt catcagcccc aaggacccgc gctggctctc ttcgctcagg cggatcgaga
      421 aggacctggt caccgactcc ctcgtctacc ggtaccgccc ggaggtggct gcccctgatg
      481 gcttcacgag ccgggaagga acgttttcga tgtgcacttt ctggtatgtg gagtgtcttt
      541 cccgcgcggg cctgctggaa aaggcgagat tttattttga aaagatgctc ggatatgcca
      601 atcacctggg cctctattcg gagcagttgg gattcgaagg cgagcatctc gggaatttcc
      661 cgcaggcgtt tacccatttg gggctgatca gcgccgcctg caatctggat gaacaattca
      721 acgataatcg gaacaaaaac atcaacctgg aagaattata attgatctat gccggtatcg
      781 caacgcaaga agcccgtaaa atccgggctc aggaaagtat taaagaccat gggccccggg
      841 ctggtcaccg gggcaagcga cgacgatcca tcaggcatcg tcacctatac ccaggccgga
      901 gcgcaattcg gtttcgcaac cttatggacc gcgctgatca cttttccgct gatggcatct
      961 gtccagggta tggcggcgag gatcgggatg gttacgagcc agggcctgac ggggaccctg
     1021 aagatgcact atccgaaggc ggtgctgtat ctcatgatcg tttcgtgctt tcccgccgtc
     1081 gtgttgaata tcggggcgga tatccaaagt atgggcgccg tggcccacct gctggcgcca
     1141 accgttccga cgtttacgtt ttgcatcctt tttacggcaa tactcctgtt cgtgatcatc
     1201 cggtattctt accgccgggt ggctgcgttg ctgaaatggc tttgtctcgc gctgctgtta
     1261 tacctgatca tcccttttct cgtccatccg gactgggggg cggtggcccg gcgtactttc
     1321 gtgccttcaa tccggctgga caaaggtttc atgagcatcc tcgtcgcgat cctcggaacg
     1381 acgatctccc cttatctgtt cttctggcag gcgaccatgg aagcggagga tatggagcat
     1441 gcgagccgca aaaggatctt tgtcgacaaa cggatgctgg gcgacatgcg gagggacgta
     1501 aacaccggga tgctgttttc gaacgtggtg acctatttca ttatcctcac ggccggagtc
     1561 gtattatttg gggccggcgt tcgccagatc gacacggtgg aacaggcggc ccattccctt
     1621 cggccgcttg ccggccagtt gagctatctc ttgtttgcga tcggcgtgat cggcaccggg
     1681 ttcctggcca tccccgtcct ggcagggtcg gtctcgtata tgttggcgga gacctttaac
     1741 ttctcaaagg ggctggacaa gaaattttat gaggcaaggc aattctatct tgtcatcatc
     1801 gtcagcctgg tggccggact ttgtctccaa ttgttcggca tcacacccgt gcaggcactg
     1861 atatatacgg ccatccttta tggactgacg gcgccggtga tgatcgtgct gctgatgcac
     1921 atcgggaaca acaagaagat catgggccgg cacacgaata gcccgctttc gaatattctc
     1981 ggggcgctga cccttttgct gatgacggct gcggcggttg ggctgatctg gctccagctt
     2041 acgtcctgat gccgcggagg ctaccgttgc tgatgatgcg gctgcttttc tctttcccgc
     2101 tcgtgaaggc cacggagctt ttcctttgcc gccgtcagta ctttttttct tttggcggga
     2161 agattttttc ccgcgcggtt gatataaaag ttaagcatgg acatcgcgga ctggtaggaa
     2221 cccgctttct tgcgcgtgct gcggtgcgcc gaccggttca gcgattgcgc gatcttcccg
     2281 gggtcttcca gcgtgaacac ccccttgtcg aggtcaaggg cgttgctgtt ccgcgtcact
     2341 ttgccggacc acctgctttt attgcccctg gcgccttttt gcttccgatg gtggttttta
     2401 gcttgcttca tgcaggaagc ggccaaaagt catgccattc tccgcgttga aaggtgttgc
     2461 gggcatagga tttgaggccg ttccagaaaa aaacaattat gggcagtacg gctgcaatga
     2521 gcaccatttc ccaggtcctc gaaagcctga gacgaaaaaa catggacctt gaattcaggt
     2581 ggacccccga aggctttaca gcggggggtg caaaatatta tcagccggag gaactggaga
     2641 tcgtcagggt cttccgtttc gaaggcgaga gcgacccgtc cgatatggcc gtcatctacg
     2701 tgttgcgcgc cgcagacggt gttaccggct atagcctcga cgcatacggc gcttacagca
     2761 gtcatgatct cgaatcgggt tataataatt tcatccgccg cattccggaa agcgaccacg
     2821 accaacagat ccttttcgag ttgtaggctc agtctccaaa agcagctgac ttcatatgac
     2881 aatgtatcct ggcgacccct atcccctggg ctccttctgg gacggaagag gggtgaattt
     2941 tgccttgtat tcgcataatg cggaaggcgt cgagctttgc ctcttcgaca agccgcaggg
     3001 cgaaaaagaa acggcccgct tcaagcttac gcagcggacg cacgatatct ggcacggtta
     3061 cctgtatgac tgcaagcccg gacaattata cgggtaccgc gtgtacgggc cctacgaccc
     3121 gtccaacggt caccggttca atccggcgaa attattgctc gatccgtatt caaaggcaat
     3181 ctgcactttc gagaaatggg acgatacgct tttcggttac gagatgggca aggatgatct
     3241 gacctgcgat acgcgggaca atgccatggt tgccccgaaa tcggtggtga tcgaccacca
     3301 gttcgactgg gaaggcgacc ggcccccgaa gatcgcctac cacaatacga tcatttatga
     3361 agcccatgtc aagggactga caaaactatt tcccggcatt ccctcaaaca tccgcggaac
     3421 ctacgcggcc ctggcgcatc caatggtgat cgattacctg aagaatctcg gtatcaccgc
     3481 tatcgagctg atgccggtgc atcagagcat tcccgagaga caccttgtcg agcaggggct
     3541 gacgaattac tggggttata attccgttgg tttttttgcg ccggggatgc gcttttacag
     3601 cggaaaggag gtcggcggcc aggtcaggga attcaagacg atggtcaagg agctgcataa
     3661 ggccgggatt gaggtttttc tggatgtggt ctacaaccat acggccgagg gaaatcagct
     3721 gggcccgacc ctggcctatc gcggcatcga caacaacgaa tattaccgtc tcgccgaaga
     3781 caaacggttc tactacgact atacgggcac gggaaatacc ctgaatacaa ggttgccaaa
     3841 tgtgctggga ctgatcatgg acagtctgcg ctactgggta ctggacatgc atgtcgacgg
     3901 cttccgtttc gatctggcgg cggcgcttgc ccgggagctg caggaggtca accgcctgtc
     3961 ggcgttcttc agcattatct accaggatcc ggtcatatcg caggttaaga tgatcgcaga
     4021 gccctgggat gtcggtgaag gcggttatca gatcgggcaa ttcccgcccg gctgggcgga
     4081 gtggaacggg aagtaccggg actgcatgcg cgattactgg cggggggctg acagtatgct
     4141 cgccgagttt gccgaacgtt tcaccggcag ctcggacctg tacaagaacg acaaccggaa
     4201 tccgaccgcg agcatcaatt tcatcacggc gcacgacggc ttcaccttgc aggacctggt
     4261 ttcttacaac gagcgtcata atgaagccaa caaggaggat aacagggacg gggagtccaa
     4321 caaccggtcc tggaattgtg gtgcggaagg gccgacggaa gaccagggca ttattgccct
     4381 ccgaaagaag caaaagcgta atttccttgc gaccctgatg ctatcgcaag gcgtgccaat
     4441 ggttgtcgcc ggcgatgagc tgggccacag tcagcagggg aacaacaacg cttattgcca
     4501 ggataacgag atctcctgga tcaactggaa ggacgg
//
LOCUS       DATGKE010000243         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_905148, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000243 DATGKE010000000
VERSION     DATGKE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905148"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VKU83_04210"
     CDS             <1..996
                     /locus_tag="VKU83_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92782.1"
                     /translation="AGASFVINDEAYVGTGINPLTPNQKVTSMFKYTYAQIPKTPYGY
                     DSAYGSWTSVTDFPGQARSNAVAFSIGNTGYLGGGLANDGFTPLNDYYSYTAGSGWTQ
                     IDSLVDQYGNSHPRYDAVAFSFDTTAFVMTGTDNYNYFNDIYRYSPTTNSWIALPAMP
                     GSKRSGAITWVYKGQGYLLTGYTNGSKWSSGTQCYDFWRFTYKSDVDTGMWGRLHDIF
                     NTNAGTFDDGYANIIRVHGVGFIELGTANGDKGYVTLGSVNGGDVTFTWEYDFATDLW
                     NEKTPYEGAARTGAVGFSLQNQTRGFVATGLNQGSTAAYSDCEEFFPTQVYNQYD"
     gene            989..1378
                     /locus_tag="VKU83_04215"
     CDS             989..1378
                     /locus_tag="VKU83_04215"
                     /inference="COORDINATES: protein motif:HMM:NF027575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HLI92783.1"
                     /translation="MTRTGLFFRKWGLSVIMLAAIIAAVWIGLDRRAKWKKDHVMVEL
                     KAIQTANGWGYDILADGHPYIHQDRIPDIRGVHGFQSKEEALAVGRKVYDRLISNQLP
                     IVSIPEMRELHVRLPEDSTDTNHLIKK"
     gene            1381..2520
                     /locus_tag="VKU83_04220"
     CDS             1381..2520
                     /locus_tag="VKU83_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92784.1"
                     /translation="MKRINYTLFVLLVAAIGFSCTRTNLDYTQNGNWASRTTFGGNAG
                     IGVGYAASFVIGNNAYVGTGVNPQFPSRKLQSVFMYTPGTFSTAVGSVDSPAAQGTWT
                     AIADFPGPARSNAVGFSAGGQGYIGTGLADDGVTIYSDFYAFNPGSGTWAKVDSIADA
                     SRSYPRYDATSFSFDSVGYVLTGTDGFNYFGDVWKFSPATGQWTQQANFPGSQRSGAV
                     TFVYHGQGFILTGHTPNSKWASSNLAYDFWRFNPSSVNPDIRWIRLRDIYNTSPGTYD
                     DGYSTIVRTNGASFVILGTEGGDKGYITCGANNSTDINFTWEYDFLSDTWDQKAPFKG
                     TDRTGAVGFTIQNRGFVATGLNAGAQAAYTDCYEFFPNMVYNPYD"
     gene            complement(2601..>2913)
                     /locus_tag="VKU83_04225"
     CDS             complement(2601..>2913)
                     /locus_tag="VKU83_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92785.1"
                     /translation="MNYIQYSRALINENKWRASRYGIDGHLIDFGKEEEVNTRVLIYE
                     LLEFVDDVLDPLNCRQAAQYATKILESGTGADRQLKVFEDSGNLIDVVDYIQKQFLEG
                     I"
BASE COUNT          639 a          787 c          840 g          647 t
ORIGIN      
        1 gccggcgcct cgttcgtgat caatgatgag gcgtatgtgg ggacaggcat caatccgctg
       61 actcccaacc aaaaggttac ctccatgttc aaatatacct atgcccagat ccctaagacg
      121 ccttacggat acgacagcgc atatggttcc tggacttccg tcaccgattt ccccgggcag
      181 gcccgcagca atgcggtcgc gttctcgatc ggcaatacag gctacctggg tggcggcctg
      241 gcgaacgacg ggttcactcc tttaaatgat tattattcgt atactgccgg cagcggatgg
      301 acgcagatcg actcgctcgt ggatcaatac gggaacagcc acccgcggta cgatgccgtt
      361 gcgttcagct tcgacaccac cgcgttcgtg atgacgggta cggacaacta taactatttc
      421 aatgacattt accggtacag cccgaccacg aacagttgga ttgccttacc cgccatgccg
      481 ggttcaaagc ggtcaggcgc cattacctgg gtgtacaagg gtcagggcta cttgttgacc
      541 ggctatacga acggttcaaa gtggtcgagc ggcacccaat gctacgactt ctggagattc
      601 acctacaaga gtgacgtaga caccggcatg tggggccggc tacacgatat tttcaatacc
      661 aatgcgggta cgttcgacga cggctacgcc aatattatcc gcgtgcacgg ggtaggcttt
      721 attgagctcg ggaccgcaaa cggcgataaa ggatacgtta cactgggttc ggtgaatggc
      781 ggtgatgtga ctttcacctg ggaatacgat tttgcgacgg atctgtggaa cgagaaaacg
      841 ccttacgagg gcgcggcccg gacaggggcg gtcggcttct cgctacaaaa tcagacaagg
      901 ggctttgttg cgacggggtt gaaccagggt tcgaccgcgg catactcgga ctgcgaagag
      961 ttcttcccca cccaggtcta caaccaatat gactaggacg ggactttttt tccggaaatg
     1021 ggggctcagc gtgatcatgc tggcagcgat cattgccgct gtgtggatcg ggttggatcg
     1081 ccgtgccaaa tggaaaaagg atcatgtcat ggtcgagttg aaagcgatcc agacggcaaa
     1141 tggatgggga tacgatatcc tggctgacgg ccacccgtac attcaccagg accggatccc
     1201 ggatatcagg ggggttcatg ggttccagtc gaaagaggag gcgctggcgg tgggcagaaa
     1261 agtgtacgac cggctgattt cgaaccagct gccgatcgtt tccatcccgg agatgcgcga
     1321 gctccacgtc cggctcccgg aagattccac ggatacaaat catcttatca agaaataggt
     1381 atgaaacgaa ttaattatac attgtttgta ctactggttg cggccatcgg attcagctgc
     1441 accaggacga accttgacta cacccaaaat gggaactggg ccagtcgcac caccttcggc
     1501 ggcaatgccg gcatcggggt cgggtacgcg gcttccttcg tgatcgggaa taatgcttat
     1561 gtggggacgg gcgtcaatcc gcaattccct tccaggaagc tccagtccgt cttcatgtat
     1621 acaccgggga ctttttcgac agcggtcggc agcgtggaca gcccggccgc ccagggcacc
     1681 tggacggcga ttgccgattt tcccgggcct gcccggagca atgccgtcgg cttctcggct
     1741 ggtggacagg gctatattgg aaccggattg gccgacgacg gcgtaaccat ctattcagat
     1801 ttttatgcct tcaatcccgg gagcggaacc tgggcaaagg tcgattcgat tgccgacgcg
     1861 tcgcggtcct acccccgtta tgacgcgacg tcattcagtt tcgattccgt cgggtatgtc
     1921 ctgacgggga ccgacgggtt caattacttc ggtgacgtct ggaagttcag tccggctacg
     1981 ggtcagtgga cgcaacaggc gaattttccc ggtagtcagc ggtcgggggc cgtcactttt
     2041 gtttatcacg ggcagggatt tattctgacg ggccatacgc ccaattccaa atgggccagc
     2101 agcaatctcg cctatgattt ctggcgcttc aatcccagct ccgtcaaccc ggacatccgc
     2161 tggatccggc tccgggacat atacaatacg agccccggca cctatgatga cggttactcg
     2221 accatcgtac ggaccaacgg agcctccttt gtaatcctgg gtacggaagg tggcgacaag
     2281 ggctatatca cctgcggcgc gaataacagc accgacatca atttcacctg ggaatatgat
     2341 tttctttcgg atacctggga tcaaaaggcg ccctttaagg gaaccgaccg gaccggggcg
     2401 gtgggtttca ccatccaaaa ccggggcttc gtcgcaaccg gtctgaacgc tggtgcgcag
     2461 gcggcttata ccgattgtta cgagttcttt ccgaacatgg tatataaccc ttacgactga
     2521 cagaagaggg aaacgatatt gcgaacgggc ctcctggccc gttttttttg ccatcccggt
     2581 catgagacgg aaacagggga tcagatccct tccaggaatt gcttctggat gtagtcgaca
     2641 acgtcgatca ggttgccgga gtcctcgaac actttcaact ggcgatcggc gccggtcccg
     2701 ctctccagga tcttcgtggc gtattgagcg gcctggcggc agttgagggg atccagcacg
     2761 tcgtcgacaa actccagcag ctcgtagata aggacgcgcg tgttgacctc ttcttcttta
     2821 ccgaagtcga tgagatggcc gtcgatacca tagcggctcg cgcgccattt gttttcgttg
     2881 atcagggccc gggaatactg gatatagttc atg
//
LOCUS       DATGKE010000244         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_953708, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000244 DATGKE010000000
VERSION     DATGKE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953708"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VKU83_04230"
     CDS             complement(<1..910)
                     /locus_tag="VKU83_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLI92786.1"
                     /translation="MSKVKTAFFCSNCGYESAKWSGRCPSCDQWNTFVEEVISKDNKL
                     SEADWRSFSPDKKENKAVSIHDVISAEEKRIVTPDQELNRVLGGGIVSGSIVLVAGEP
                     GIGKSTLFLQNGLRLSGTKTLYISGEESEQQIKMRADRLNIRNDAFYLLTETSTSTIF
                     QEIKKLRPDLVIVDSIQTLQSPYIESSAGSVSQIRECAAEFQRFAKDSGTPVFLIGHI
                     TKDGSIAGPKILEHMVDTVLQFEGDRHYAYRILRTLKNRFGSTAELGIYEMTGEGMRG
                     VLNPSELLITQKEEQLSGIAIAASMEG"
     gene            1726..>2149
                     /locus_tag="VKU83_04235"
     CDS             1726..>2149
                     /locus_tag="VKU83_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92787.1"
                     /translation="MRKNRHFLTGILLLVIVGLHAQTIEITGKVTDATGAPIPAATIR
                     VKNAKGGTSADFQGNFTIKAAKNAILIISGVGYETREVKVTGPTVEIQLPTDSKSLSE
                     VVVTGTGVATSKRKLGISVESVTADKLPPAPTASIDQAL"
BASE COUNT          564 a          539 c          481 g          565 t
ORIGIN      
        1 ggccttccat tgaggcggca atcgcaatcc cgctaagttg ttcttctttc tgggtgatca
       61 gcagctcgct cggattcagc acgccgcgca tcccttcgcc cgtcatttca tagatgccaa
      121 gctcggcggt gctgccaaaa cgatttttca gcgtccggag aatgcgatag gcataatgcc
      181 ggtcgccttc gaattgcagt acggtatcga ccatgtgctc gagtattttt gggccggcga
      241 tgctcccgtc cttcgtaata tggccgatca ggaaaacggg cgtgccggag tcttttgcaa
      301 accgctggaa ctccgccgcg cactcgcgga tctgagaaac gctgcctgcc gaggattcaa
      361 tgtaagggga ctgcagcgtt tggatcgagt ccacgatgac caggtcgggc cgaagctttt
      421 tgatctcctg aaatatagtg gatgttgatg tttcggtaag gagataaaat gcatcgttcc
      481 tgatgttcag cctgtccgcc cgcattttaa tctgctgttc gctttcttcg ccggaaatat
      541 acagtgtctt cgtgcctgat aaccgcagcc cgttctgaag gaacagcgtg cttttgccaa
      601 tgcctggttc gccggctacg agaacaatgc tgcctgaaac gattccgccg cccaatacgc
      661 ggttcaattc ctggtcggga gtgacgattc gtttttcttc ggcgctgatc acgtcgtgga
      721 tcgaaacggc cttgttctct tttttatccg gactgaaaga tcgccagtcc gcttcggaaa
      781 gtttgttgtc cttactgatc acctcttcaa cgaacgtatt ccattgatcg catgaaggac
      841 atcttcccga ccatttcgcg ctttcgtaac cgcaattgct gcaaaaaaaa gctgttttga
      901 ctttgctcat ccggtaaagt tagccgcttg ccgttccata aattggaaaa acatcaatca
      961 tcgaatggtg ggagaaatcg ggctttggcc tgaaagtaaa agggccagct taatcgccgg
     1021 ccccttactt actaacccat taaattcttg cactaaatta caaattcgcg ccacataaca
     1081 aaataaattt ttctccttgt gaataacttt gaaacgccgg aacgagcttt ggcgaagatt
     1141 aaaacaactg gtataattaa ctggaaatca ataatttaca caaatatgcc aagtatgtct
     1201 ctgaagagat cttcatcttt aaacggttga gaattagccc ccattagact gccataaaac
     1261 ccaaaaaaaa tttttcacca gaccggtttc gggtagccaa aattcgcctc attccttcgc
     1321 cacaaaatag cataataaat tgattgatat atttacgaat atctattaac ggcaagtttc
     1381 ggcgtaggaa ggtccagaag gtcatattta ttttaaattt aataacttcc cgggccacct
     1441 gatcgtccga aggttcgcgc ccggcccctt ttttactaaa atgctatatc tagtcctaaa
     1501 atataatatg tcagtgaacc ctaaacgttt tttgcaagaa tgttatcaaa taaatgggaa
     1561 cagaattgaa ttttttctaa ataaaatttc caaagattaa tttaggatta aaaatccgta
     1621 ctttattaaa ccaagactga cggctgccac tggagtaccc ctttcgtcat tcgagggttt
     1681 gttgttgcat tgaggtcaca attcagtcac caacaacatc tacacatgag aaaaaatcgt
     1741 cacttcttga cgggcatact cctgctcgtc atcgtggggc tgcatgcgca aaccattgag
     1801 atcaccggta aagttaccga tgcgacaggc gccccgattc ccgccgctac catccgggtc
     1861 aagaatgcaa aaggaggtac cagtgcggac tttcagggca attttacgat caaggccgcg
     1921 aagaacgcca tcttgatcat cagcggcgtc ggctatgaaa ccagggaggt aaaggtaacc
     1981 ggcccaacgg tcgagatcca gcttccaacc gattccaaat ccctaagcga ggtggtcgtt
     2041 accggtaccg gcgtggccac cagcaaacga aaactcggca tctccgtcga gtcggttaca
     2101 gcggacaaat tgccgcctgc cccgactgct tctattgacc aggcactga
//
LOCUS       DATGKE010000245         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_829058, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000245 DATGKE010000000
VERSION     DATGKE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829058"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2909
                     /locus_tag="VKU83_04240"
     CDS             <1..>2909
                     /locus_tag="VKU83_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HLI92788.1"
                     /translation="IRVLCSPQTLSEAEKYLRTQRAQIDYARDEVVKASVEIDGQVYQ
                     VVIRKNEERNFDTSSDYPDPTHPLNLPKVILFLQLLHAHGAYYFETIRNWDTEKNKLL
                     EAYGYSLSDDLKGKFEFTYKEGKPFLRVLDAAIKRVAPLASSRPRAVEAEVPSMLMPE
                     EDKAVGNLQRTGIVFHFNHDGFPGFALDAVTGESNEDQTAYVGKVEKMDLTKFVNVEP
                     FPESDRLLIPSLRKMQTQEINKYLNRNSPFSGIWENIIHHEEDDLPEETKKLMVEYLH
                     PKLRKLFSELHSSPFVYFVDGRAAFKTANMQPMGLSADTISPQFSVSGGRSGYELSCF
                     VKINGQAVDIAKNESSSPVIFLYNNNGYLWNRPEDVLLVEKFAGKEPRVIGRGEWPER
                     MKDLILPLTRDYTVEFDQELVREIRDGDPDRKLLLQEKGDYLLFQPVFSYKGFETRPG
                     GKDEIIVPDGQQVLIVHRNKEAELQFFRKLESLHSQFIRPEGSHSLALKGTDVLKNNW
                     FFLFVDAMEEMKVPVYGFDALKNFRFNTAKPQTKIHISSNTDWFDARVDIVFGDQRVT
                     IADVKKALGNRQQFVPLHDGTLGVLPEEWIKKYSLLFRVGEGKSNQLRLSKYHMSVID
                     ELYDNRNEEELIIRLEEKYEQLREFNRIREVPPPEHLLPILRPYQVHGFHWLNYLQDV
                     RWGGILADDMGLGKTVQALSFLQHYRDVNAQLTALVVCPTTLMYNWENEIRKFTPTLT
                     YHIHHGGDRTRNKEVLEGFNVIITTYGTLRSDIKLLLEINFDYAVLDESQAIKNPSSK
                     VTKAACLLQAKNRLCMSGTPLQNNTFDIFAQMNFLNPGMLGSLEFFRQEFAVPIDKFG
                     EQDRKEHLRKLLYPFILRRTKEQVAKDLPDKTETILFCEMEDEQRKVYEAYRNDYRDK
                     ILGTIETQGIQRSQLTILQGLMKLRQICDSPAILNETDKYPNHSIKLDELAREIA"
BASE COUNT          698 a          804 c          819 g          588 t
ORIGIN      
        1 ccatccgggt gctctgctcg ccccagactt tgtccgaggc cgaaaagtat ctgcggacgc
       61 aacgggcgca gatcgattat gcccgggacg aggtggtcaa ggcgtcggta gagatcgacg
      121 ggcaggtcta ccaggtggtc atccgcaaga acgaagaaag aaatttcgat accagcagcg
      181 attatccgga tcccacccat ccgctgaacc ttcccaaggt cattctgttc ctccagctgc
      241 tgcatgcgca cggcgcctat tatttcgaga cgatccgcaa ctgggatacc gagaagaata
      301 agctgctgga agcctacggc tattcgcttt ccgacgacct caagggtaag ttcgaattca
      361 cctacaagga gggcaaaccc tttctgcgcg tgctggacgc ggccatcaaa agggtcgctc
      421 cgcttgcgag cagccggccc cgggcggtcg aggccgaagt gccgtcgatg ctgatgcccg
      481 aggaggacaa ggcagtcggc aacctgcagc ggaccggcat tgtctttcat tttaaccacg
      541 acgggtttcc cggctttgcc ctggatgccg tcaccggcga aagcaacgag gaccagaccg
      601 cctatgtcgg gaaagtggaa aagatggacc tgacgaaatt cgtgaacgtg gagccttttc
      661 cggaatccga ccgcctgctc atcccctcgc tgcggaagat gcagacgcag gagatcaaca
      721 aatacctcaa ccggaattcc ccttttagcg ggatctggga gaacatcatc catcatgagg
      781 aggacgatct tccggaagag accaagaagc tgatggtaga gtatcttcac cccaagcttc
      841 gaaagctttt ttccgagctc cattccagcc cctttgtata tttcgtcgac gggcgcgcgg
      901 cgttcaagac cgccaacatg cagccgatgg gcctttcggc ggatacgatc agcccccagt
      961 tcagcgtgag cggcggccgg tccggctacg agctttcctg tttcgttaaa attaacggcc
     1021 aggccgtgga tatcgccaag aacgaatctt ccagcccggt cattttcctt tacaataata
     1081 acgggtatct ctggaaccgg ccggaggatg tgctgctcgt cgagaaattc gccgggaagg
     1141 aaccgcgggt gatcggccgc ggcgagtggc cggaaagaat gaaggacctg atccttcctc
     1201 tcacgcggga ctataccgtc gagtttgacc aggaactggt gcgcgagatc cgggacgggg
     1261 acccggatcg aaaactatta ctgcaggaaa aaggcgatta cctgcttttc cagccggtct
     1321 tttcctacaa agggttcgag acccgccccg ggggtaagga tgagatcatc gtgccggacg
     1381 gtcagcaggt gctgatcgtg caccgcaaca aagaagccga attgcagttt ttccgcaagc
     1441 tggaatcgct gcattcgcag tttatccggc cggaaggctc gcatagcctg gcgttgaaag
     1501 gcacggacgt cctgaagaac aactggttct tcctgtttgt cgacgccatg gaggaaatga
     1561 aagtccccgt ctatggattc gatgccttaa agaatttccg tttcaatacc gcgaagccgc
     1621 agaccaagat ccatatcagc agtaataccg attggttcga cgcaagggtc gacatcgtat
     1681 tcggcgacca gcgggtgacc atcgccgacg tcaaaaaggc cctcggaaac cggcagcaat
     1741 tcgtcccctt gcatgacggc acactcggcg tattgccgga agagtggatc aagaaatatt
     1801 cgttgctttt ccgcgtcggc gagggaaaga gcaaccagct tcgcttgtcg aaatatcata
     1861 tgagcgtgat cgatgagttg tacgacaacc gcaacgaaga ggagctcatt atccggctgg
     1921 aagagaaata cgagcaactg cgcgaattca accgtatccg ggaggtcccg ccgccggagc
     1981 acctgctgcc gatcctgcgg ccttaccagg tgcatggatt ccactggctg aattacttgc
     2041 aggacgtcag gtggggcggc atcctggcgg acgatatggg cctggggaaa accgtccagg
     2101 ctttgtcctt tcttcagcat taccgggatg tgaacgcgca gctcaccgcg ctggtcgttt
     2161 gcccgaccac cctgatgtac aactgggaga acgagatccg gaaatttacg cccaccctga
     2221 cctaccatat tcatcatggc ggggaccgga cccgcaacaa ggaagtgctg gagggattta
     2281 acgtcattat taccacctac ggcacgctgc gaagcgacat caagctgctg ctggagatca
     2341 attttgacta tgcggtcctc gacgaatcgc aggcgataaa aaacccatcc agcaaggtga
     2401 ccaaggctgc ctgcctcctg caggcgaaga accggctgtg tatgagcggt acgccccttc
     2461 agaataatac cttcgacatt tttgcccaga tgaatttcct gaacccgggc atgttgggca
     2521 gcctcgagtt tttccgccag gaattcgcgg tcccgatcga taagtttggc gaacaggaca
     2581 ggaaggaaca cctgcggaaa ctgttgtatc cgttcatcct gcggcggacc aaggagcagg
     2641 tggccaagga tctgcccgac aagaccgaga ccatcctctt ctgtgaaatg gaggacgaac
     2701 agcggaaggt ctatgaagct tacaggaacg attaccggga taagatcctt ggcacgatcg
     2761 aaacgcaggg tatccagcgg tctcagctga cgatcctgca gggtttgatg aaactgcgcc
     2821 agatctgcga ttcaccggcc attctgaatg aaacggacaa atatcccaat cattcgatca
     2881 aactcgacga gctggcccgg gagatcgct
//
LOCUS       DATGKE010000246         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1300672, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000246 DATGKE010000000
VERSION     DATGKE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300672"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VKU83_04245"
     CDS             complement(<1..485)
                     /locus_tag="VKU83_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92789.1"
                     /translation="MKQLILLFCTYLPLGQIIAQGPPAGGPLPAAAGRPGGAGAAGGN
                     SASGHLYGKLVDSAGHGVADASVMVLGPVKDPVTGKRHESLLKGVSTQNNGDFSVEDL
                     PVNTALKLSVSAIGYRVFTREITLAARDADKDLGKIPMAQASSELQQVVVTASRPAMS
                     V"
     gene            complement(595..1377)
                     /locus_tag="VKU83_04250"
     CDS             complement(595..1377)
                     /locus_tag="VKU83_04250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLI92790.1"
                     /translation="MKQVIFLAGCFTILTCHAQHPDAWNHKSCAVVLTYDDAIEADLD
                     NVIPALDSMGLKGTFYLIGSSPVIANRMVEWRKAAADGHELGNHSMFHPCDGSLPGRG
                     FVSPDNDLSKYFVRRAVAEIRQTNVLLQAIDGRKRRTFAYPCGDRTIHDTLFYNWLQK
                     DFVAARGVTGKLDSLGKVDLDNVDCFAMNGQTGDQMIALVRRAMQTHTLLVFLFHGVG
                     GGHNLNVGLRDHSELLHFLHDHKQEIWIAPMVDVAEYIRAHQ"
     gene            1431..>2285
                     /locus_tag="VKU83_04255"
     CDS             1431..>2285
                     /locus_tag="VKU83_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HLI92791.1"
                     /translation="MANNKLVFILSLFAAPLISPAQVRLPGLFTDNMVLQRDIPIRVW
                     GWAAPGESLRLEWEGRKFRTHAKTDSTWEVTLPAMKAGGPYVLDIRGKNRLVIRNILV
                     GDVWFCSGQSNMVLPMERVKEKYPQDISAANFSQIRSFSVPALADVTKVHEDLPGSDW
                     REANPQNVLAFGAAAYFFARDLYEKYHVPVGIINSSVGGTPIQAWISAEGFREMPDYS
                     NRIARFEDTAYIAAIRRAAMPHREKPERPDAGLSGPVKWYDPAYVPVDWHPFWMPGYW
                     ADQGVRGLN"
BASE COUNT          482 a          616 c          692 g          495 t
ORIGIN      
        1 tcgaccgaca tggcgggcct ggatgccgtc actacaacct gctgcaattc gctgctggcc
       61 tgtgccatcg gaattttgcc aaggtccttg tctgcatcac gggccgcgag ggtgatctcc
      121 cgggtgaaca cgcggtagcc gatcgccgag acgctgagct ttaaggctgt gttcaccggc
      181 aggtcttcta cggaaaagtc gccattgttc tgggtactta ctcctttcag gagcgactca
      241 tggcgcttgc cggtgaccgg gtccttaacc ggaccaagaa ccatcaccga tgcatcggcg
      301 acaccgtgac cggcggagtc gacgagcttg ccgtaaaggt ggcctgatgc ggaattaccg
      361 cctgcggctc cggcgcctcc cggacgtccg gcggctgcgg gaaggggacc accggcgggc
      421 ggcccctggg caatgatctg gccgagtggc aggtaggtgc agaaaaggag tattagctgt
      481 ttcatgcggt aaaactaata cgcccttccg gggtcctcac ggccattgcg gggaaccggg
      541 aaaacattgc ggtgaaccgg caaaacaacg gtgaagcagg cttgaccgcg cctgctattg
      601 atgcgcgcgg atatactccg ccacgtcgac catcggcgcg atccagatct cttgtttatg
      661 atcatggaga aagtgcagca gctcgctgtg gtcgcgcagg ccgacgttga gattgtggcc
      721 ccctcctacc ccatggaaga gaaaaacgag gagcgtatgg gtctgcatcg cgcggcggac
      781 aagggcgatc atctggtcgc cggtctggcc attcattgca aaacaatcca cgttatccag
      841 gtccaccttt ccaagggaat ccagctttcc ggtgacgccg cgggcggcga caaagtcttt
      901 ttgcagccag ttataaaaca gcgtgtcatg gatcgttctg tcgccgcagg gataggcaaa
      961 ggtccggcgc ttcctgccgt cgatggcctg gaggaggacg ttggtctggc ggatctcggc
     1021 cactgcccgc cgcacgaaat atttgctgag gtcgttgtcc ggggagacga atcctcgtcc
     1081 tgggaggctg ccgtcacagg gatgaaacat cgagtggttg cccaactcgt gtccgtcggc
     1141 agccgcttta cgccattcca ccatcctgtt ggcgattaca ggtgaagacc cgatgaggta
     1201 aaacgtccct ttcagtccca tcgagtccag ggcgggaatg acgttatcca aatccgcttc
     1261 gatggcgtcg tcgtaggtca gcaccaccgc gcaagactta tggttccagg cgtcgggatg
     1321 ttgggcatgg caagtgagga tggtaaagca gcctgctaaa aaaattactt gtttcatcat
     1381 ttgcgataaa actactgaaa taattgtagt ttcatgcaac cgattgcagc atggccaata
     1441 acaaattagt tttcattctc agcctcttcg ctgcgcccct gatttccccc gcgcaggtta
     1501 ggcttccggg attattcaca gacaatatgg ttttgcagcg ggatattccg atcagggtat
     1561 ggggctgggc ggcgccgggc gaaagcctca gactggaatg ggaaggcagg aagttccgga
     1621 cacacgcgaa gaccgatagt acctgggagg ttacgctgcc cgccatgaaa gcaggtgggc
     1681 cttacgtgct tgacatccgc gggaaaaatc gcctcgtaat tcgcaatatt cttgttggcg
     1741 acgtctggtt ttgttcggga caatccaata tggtgctgcc gatggaaagg gttaaggaaa
     1801 agtacccgca agacatttca gcggcgaatt tttcgcagat ccggagcttt tctgttccgg
     1861 cattggccga cgtcacgaaa gtccatgagg atctccccgg ctcggattgg cgggaagcca
     1921 acccccagaa tgtgctggcc tttggcgctg ccgcctactt ttttgcgcgg gacctgtacg
     1981 aaaaatacca cgtgccggtc gggatcatca attccagcgt cggcggtaca cccatccagg
     2041 cctggatcag cgcagagggc tttagggaaa tgccggatta cagcaacagg attgcaaggt
     2101 ttgaggatac tgcctatatc gctgcaatcc gtcgcgcggc aatgccccat cgtgaaaagc
     2161 cggagcgtcc cgatgcgggt ttatccgggc cggttaaatg gtatgatccg gcgtatgtgc
     2221 cggttgactg gcatcccttc tggatgccgg gttattgggc ggaccaggga gtgaggggcc
     2281 tgaac
//
LOCUS       DATGKE010000247         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_805965, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000247 DATGKE010000000
VERSION     DATGKE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805965"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VKU83_04260"
     CDS             complement(<1..707)
                     /locus_tag="VKU83_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLI92792.1"
                     /translation="MNKAEINKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGAVKS
                     NKIKKHATSDFMEIERQRGISVATSVMSFEYNGMLINLLDTPGHKDFAEDTYRTLTAV
                     DSVILVIDSVNGVEAQTRRLMEVCRMRDTPVIVFVNKMDRDGKNRFDLLEEIEKELNI
                     QLHPMTWPINSGKDFKGVYNLHDKNLVLFAANSRASEGDEDTLRIDDLGSPVLDERLG
                     QKDAATLREDVELVDGV"
     gene            complement(737..2254)
                     /locus_tag="VKU83_04265"
     CDS             complement(737..2254)
                     /locus_tag="VKU83_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HLI92793.1"
                     /translation="MSTIRKQSIISSGLVYIGFGLGFLYTILFARGFTPGQYGLTQMF
                     LALGSIMYYVANLGMPSFIYKFYPYYRDHLSPRRNDMLTWALIVTIIGFLFVVAGGLI
                     FKGFVIRKFSNESPGVVRYYAWMFPYGFGLSLYSLLEAYAWQLKRSVLTNYFREVQLR
                     VFNILLVVLYYVGWLKGFDAFVKLYALGYLVLAICLITYLVLHRELHFTMRVSRVTRR
                     FFRKIVAQASLVWSGQLLYNVSLFFAQIVIAAVVPGGLKYVGIYSLAQIVASLIQAPQ
                     RGIIAASIGPLSQAWKNKDYARIDRIYKRSSINQLIFSVGMFILIWLNFRDGVLTFHL
                     KLDYLEAQRPFLFIGLMKIIDMGTGVTNQIIGTSTFWRFDFFTGVILTALTLPVNYFL
                     TKTVGFTGPAIADLVTFTLYNAIRWIFLYRKFDMQPFDLKSVYTLLLGGIGWWLCEFL
                     FGRAGGFGSIVLRSLVFIAIFGGGTLALRLSDDVGPVWMTVKKRLAAAMGRPRKV"
     gene            complement(2263..2736)
                     /locus_tag="VKU83_04270"
     CDS             complement(2263..2736)
                     /locus_tag="VKU83_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HLI92794.1"
                     /translation="MRDLKLVGLIVLIGLAGCRQPEPLDYYGFQNLQVSAVVGGQTDV
                     SATAKLYNPNRFSVKVKRAEVDIKVNGQHAGHSLLDSTIFIPARDTFYVPVTLQLDMR
                     GLFNNALQMLMGKREATVDLDGKVKINRGLITFSRKFHYEGKQDLSSLMSAGQDF"
     gene            2801..>3306
                     /locus_tag="VKU83_04275"
     CDS             2801..>3306
                     /locus_tag="VKU83_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HLI92795.1"
                     /translation="MPTIAERVLDFNKDRLPVYTALKYQSMAENPFRFFRGTCHLFFE
                     DLAQRNALPAYPLTWVCGDLHLENFGSFKGSNRLVYFDLNDFDESALAPATWELARML
                     TSIFTGFDAVGIRKKDATQTAQLFLRVFSATLSKGKARYLEHETAKGIVRNFLEKIAE
                     RRQKDLVRL"
BASE COUNT          824 a          898 c          829 g          755 t
ORIGIN      
        1 tgtacgccat ccacgagttc cacgtcttcg cgtaaagtgg cagcgtcttt ctggcccagc
       61 cgttcatcca ggaccggact gcctaaatca tcgatccgca gcgtgtcttc atccccttcg
      121 ctcgccctgc tattggccgc aaaaaggacg aggttcttgt cgtgcaggtt atacactccc
      181 ttgaaatcct tgccgctatt gattggccag gtcattggat gcagctggat gttaagctcc
      241 ttttcaatct cctccagcag atcgaagcgg ttcttaccgt cgcggtccat tttattcaca
      301 aacacgatca ccggcgtatc ccgcatgcgg cacacctcca tcaggcgccg ggtttgcgct
      361 tcaacgccgt tcacgctgtc aatgaccagg ataacgctgt ctaccgcggt cagcgtacga
      421 taggtatcct cggcgaaatc cttgtgaccc ggcgtatcca acaaattgat tagcatgcca
      481 ttatattcaa agctcattac ggaggttgca accgagatgc cgcgctggcg ttcgatctcc
      541 ataaagtcgc tcgtcgcgtg cttcttgatc ttgttgctct tgaccgcgcc ggccgtctgg
      601 atggccccgc caaaaaggag gaatttttca gtaagggtcg tcttccctgc gtcggggtgg
      661 gaaatgatag cgaatgtccg tcgtttgttg atctctgcct tattcattat aatggggcgc
      721 aaaaataaga ataaagtcag actttgcgcg gtcttcccat ggcagcggcg agccttttct
      781 tcaccgtcat ccaaactggg ccgacgtcgt ccgacagccg cagggccagg gtgccgccac
      841 caaatatggc gataaagacg aggcttctca ggacgatcga gccgaagccg ccggcacggc
      901 caaataggaa ctcgcaaagc caccagccaa tgccgccgag cagaagggta tatacgcttt
      961 tgagatcaaa tggctgcatg tcaaactttc gatagagaaa aatccagcgt atggcgttgt
     1021 agagagtaaa cgtgaccagg tccgcgatcg cgggaccagt aaaaccaacg gtcttggtta
     1081 aaaaataatt taccggtagt gtaagggcag tcaggatgac gccggtaaaa aaatcgaacc
     1141 gccaaaacgt agatgtacca atgatctggt ttgtgacccc cgtacccatg tcgataattt
     1201 tcattagccc gatgaaaagg aagggccttt gtgcctcgag ataatccagc ttcagatgaa
     1261 aggtcaatac gccgtctcga aaatttagcc aaatgagaat gaacataccg accgaaaaga
     1321 tcaactggtt gatcgatgac cgcttataga tcctgtctat ccgggcgtag tccttattct
     1381 tccatgcctg cgacaatgga ccgatggagg cggcaattat cccgcgctgt ggcgcctgga
     1441 tcagactggc gacgatctgg gcgaggctgt aaatacctac gtatttcagc cctccgggaa
     1501 cgaccgcagc aatcacgatc tgcgcgaaga aaagtgacac gttatataac agttgtccgc
     1561 tccacacgag gcttgcctgc gctacaatct tgcggaagaa ccgccgggtg acacggctga
     1621 cgcgcatggt gaaatgcagc tcgcggtgca ggacgaggta ggtgatcagg cagatcgcaa
     1681 gcacgaggta gcctagcgca tacagcttga cgaaggcatc gaagcccttt agccagccga
     1741 cataatatag gacgacgagc aggatattga atacgcgcag ctgcacttca cggaagtaat
     1801 tcgtaagcac ggatcgcttc aactgccagg catatgcctc aagcagggaa tagaggctta
     1861 gtccgaaacc gtaagggaac atccaggcat aataacggac gaccccggga ctttcgttgc
     1921 tgaatttccg gatgacgaaa cccttgaaaa tcagtccgcc ggcgactaca aatagaaacc
     1981 cgatgatggt gacgatcagc gcccaggtca gcatgtcgtt cctgcgcggc gaaagatgat
     2041 cccgataata gggatagaat ttgtaaatga aagatggcat tccgagattt gccacatagt
     2101 acatgatcga ccctaacgca aggaacattt gggtcagccc atactggccc ggcgtgaaac
     2161 ccctcgcaaa caggatggta tataaaaagc ccagtccaaa gccgatgtac accaagcccg
     2221 acgaaataat gctttgcttg cggatggtac tcattggaat acttaaaaat cctgtccggc
     2281 ggacatcagg ctgctcagat cctgtttgcc ttcgtaatga aacttcctgg aaaacgtgat
     2341 caatcctctg ttaatcttca ctttcccgtc caggtcgacg gtcgcttcgc gctttcccat
     2401 cagcatttgt agtgcgttgt tgaataaacc ccgcatgtcc agttgcagcg taacgggcac
     2461 ataaaaggta tctctcgccg ggatgaatat agtggaatcc agaagcgaat gccccgcgtg
     2521 ttgcccattc actttgatgt ccacttccgc tcgctttact tttacgctga accggttcgg
     2581 attatataat ttggcggttg cactgacatc ggtctggcca cccactaccg cactcacctg
     2641 taagttctga aagccataat aatccaacgg ttccggctgc cggcatccgg ccaggcctat
     2701 cagcacgatc aggccgacta gtttgaggtc acgcatcggc tgcaagataa ggcgattcag
     2761 gaaatcctga ataatactta tctttcaaaa aactttggct ttgccaacaa tcgcggagcg
     2821 cgtcctcgac ttcaacaagg accggctgcc ggtctatacc gcgctgaaat atcaatccat
     2881 ggcggaaaat ccttttcggt tcttccgcgg cacctgtcac ctctttttcg aggacctggc
     2941 ccaacgcaac gcgcttcctg cctatccgct gacctgggtc tgcggcgacc ttcacctgga
     3001 gaatttcggc agctttaaag gctccaatcg ccttgtctat ttcgacctga atgattttga
     3061 cgaaagtgcc ctggcgcccg caacctggga gctcgcccga atgctcacca gcattttcac
     3121 cgggtttgac gctgttggca tcaggaaaaa agatgctacc caaacggcgc agctttttct
     3181 gcgcgtattc tccgccactc tttcaaaagg gaaagcccgg tacctggagc atgagaccgc
     3241 aaaagggatc gtgcggaatt ttctcgaaaa gattgccgag cggaggcaaa aggacctcgt
     3301 ccgcct
//
LOCUS       DATGKE010000248         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1071712, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000248 DATGKE010000000
VERSION     DATGKE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071712"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..462)
                     /locus_tag="VKU83_04280"
     tRNA            complement(389..462)
                     /locus_tag="VKU83_04280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Arg,seq:tct)
     gene            638..1312
                     /gene="ung"
                     /locus_tag="VKU83_04285"
     CDS             638..1312
                     /gene="ung"
                     /locus_tag="VKU83_04285"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLI92796.1"
                     /translation="MDVKIEDSWKQALRQEFEKPYFGKITSLLKAEKMSGKVIYPPGS
                     LIFNAFNSTPLERVKIVLLGQDPYHGPGQAHGLSFSVPKGIPPPPSLINIFKELHNDI
                     GMQIPTHGNLTHWAEQGVLLLNASLTVRANEPMSHAGIGWSEFTDAVIRKVSDRKAHV
                     VFLLWGKFAQEKQALIDETKHLVLKAAHPSPFSADRGFFGCRHFSRANAWLMSKGIDP
                     VDWALN"
     gene            1324..2076
                     /locus_tag="VKU83_04290"
     CDS             1324..2076
                     /locus_tag="VKU83_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLI92797.1"
                     /translation="MRTRYGIWNILGRIWAAWVIIIFVVTMLLFMIPFLLFCYFRRDP
                     EKTRWFTRYSRIWMGVFLPLAGCPLRINGREKFAPGKTYIVVCNHNALIDVPVTAPGI
                     PGGNKTIAKIEMAKIPLFGLMYRTGSVLVDRKNETSRRESFVRMKEALDMGLHMCIYP
                     EGTRNKTAEPLKSFHDGAFRLAITTGHEIIPAVIFHSRTIMPAGKTFYFRPHRLVMDF
                     LDPVTPGPEETVESLKQRVFVLMRDYYLAGPR"
     gene            complement(2078..>3555)
                     /locus_tag="VKU83_04295"
     CDS             complement(2078..>3555)
                     /locus_tag="VKU83_04295"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HLI92798.1"
                     /translation="VSANHNQNNETRQINVSLPNISFTAPTLYPLQKSNFIGTPKWYE
                     KLGVGLSSTITGNVSFYDSLLSLRRIDSLFQWGAQNSIPISLALPIKGPVQVTPGVSL
                     QERVYSRRLYRHYDPAFNKVDTDAIQRGVFQEESMSYSLSLATAIFGTYQGFGRNSSL
                     MGIRHVIRPTVSLSYTPDFAAKDWYTIRRPFTPGDPAKTTVTQRVSYYDGSTYGPFSE
                     GTFGGLSFQLDNHLEIKTRSKTDTTSAGIKKTTIIDGFGVSGSYNYLADSFKLSRLTL
                     YFRSTLFKNINITGGATLDPYQHNVFLNQDIDRYAWEGKGFSLGRFTSGNLAISTSFK
                     SAPNDQKLADEKAAQASDQIPMTMEEQQARLNYIRQNPAEFADFNIPWQVNLSFAFNF
                     TNQQQSNYTYVTTITSSINWNGDFNLTQNWKIGLNGYYDVKAGQMQSLTASITRDLHC
                     WHMSINVTPIGYTHMFSITINPKAGILQDLRVNRTKFFYNE"
BASE COUNT          779 a          993 c          972 g          811 t
ORIGIN      
        1 cggtcacgtc gatgttattt tgtcaactag tattataaag tgtccggatt cttacacgaa
       61 gaagggaaat tattaaacaa ctaggtgcta ttttgctttt tttggttttt tgcatttgtc
      121 cgctcacgtg agcaagcaga gcttcgcttc gctcggccac gctcccaaat aaaatagccc
      181 cgcaccatcg tacgaggctt tgtgacccgg gcgggattag ctccggcgcc ctccggccgc
      241 ctccgctgat cccaacgccc tgcgagggcc ttacccaagc gcccctcggc gcctaccggc
      301 gccgggtttt tgcattcgtc cgctctggcg aacaagttcg ccgcgctccc gaaatgcaaa
      361 aacccccgac tatcgtcggg ggcacttgtg acccgggcgg gattcaaacc cacgaccacc
      421 tgcttagaag gcaggagctc tattcagctg agctaccggg tcgtattcaa ctatgcactg
      481 gccggaacca aaacctcgtt accgccacca ctcaggactc ccttctgacg ccagtcaggc
      541 cgcgaaaata attaaaaatc ggcaggaggc caaacgggcc tgaaccctgg cgccgcagcc
      601 acaggaagcg agttttactt atcttcggcc cgacagtatg gacgtcaaaa tcgaagacag
      661 ctggaagcag gccctgcgac aggagttcga aaaaccttat ttcgggaaga tcaccagcct
      721 tctaaaagca gaaaaaatga gcggcaaagt gatttacccg ccaggtagtt taatcttcaa
      781 tgctttcaat tccacgccgc tggagcgcgt caagatcgtt ttgctcgggc aggatcctta
      841 ccatggcccc gggcaagcgc atggactgag tttttcggtt ccgaagggca ttcctccgcc
      901 tccatccctg atcaatattt tcaaggaatt gcacaatgat atcggcatgc agatacccac
      961 ccacggcaat ctcacgcatt gggccgaaca aggcgtgctg cttctcaatg catccctgac
     1021 cgtccgcgcc aatgagccca tgagccatgc agggatcggc tggtctgaat ttacggacgc
     1081 ggtgatccgg aaggtctccg accgcaaggc ccacgtcgtc ttcctcctat ggggcaaatt
     1141 cgcccaggaa aagcaggcgc tcatagatga aacgaaacat ctcgtgctga aagccgcgca
     1201 cccctctcct ttctccgcgg accgggggtt cttcggctgc cgccatttct ccagggcgaa
     1261 tgcgtggctc atgagcaaag gaatcgatcc agtagactgg gcactgaact aaaagcaacc
     1321 aatatgcgaa cccgatacgg catctggaat attcttggca ggatctgggc agcctgggtg
     1381 atcattattt tcgtggtcac catgctgttg ttcatgattc ctttccttct cttctgctat
     1441 tttcgccggg atccggaaaa gaccaggtgg ttcacgcggt attcgcgcat ctggatgggg
     1501 gttttccttc cgcttgcggg ctgcccgcta cgcataaacg gcagggaaaa attcgccccc
     1561 ggtaagacct acatcgtcgt ttgcaaccac aacgcgctga tagacgtgcc cgttaccgcg
     1621 cccggcatcc ccggtggcaa caaaacgatc gccaaaattg aaatggcaaa gatcccgctg
     1681 ttcgggctga tgtaccgtac aggcagcgtg ctggtggatc gaaagaacga aacgagccgg
     1741 cgcgaaagct tcgtaaggat gaaagaggcg ctggacatgg gcctgcatat gtgtatctac
     1801 cccgagggta cgcgcaataa aaccgccgaa ccactcaaat cttttcacga cggcgctttc
     1861 cgactggcaa ttacgacagg acacgagatc atcccggcgg tcattttcca cagccggacg
     1921 atcatgcccg ccggcaagac gttctatttc cgtccccacc gcctggtcat ggacttcctc
     1981 gatcccgtga ccccggggcc ggaagaaacc gtggaaagcc tgaaacagcg ggtatttgtc
     2041 ctcatgcgcg actattatct cgccgggccg cgatgagcta ttcgttatag aagaatttgg
     2101 tccggttgac cctgaggtcc tgcagaatgc cggccttcgg attgatggtt atgctgaaca
     2161 tatgggtgta gccgatggga gtaacgttga tggacatgtg ccagcaatgc agatcccgcg
     2221 taatcgacgc cgtcagcgac tgcatctggc ccgctttcac gtcataatac ccgttcagcc
     2281 cgatcttcca gttctgcgtc aggttgaaat cgccattcca attgatgctt gaggtgatcg
     2341 tcgtcacata ggtataattg gattgctgct gattcgtgaa attgaaagcg aacgaaaggt
     2401 tgacctgcca ggggatattg aaatccgcga attcagccgg attctgccgg atataattca
     2461 accgagcctg ctgttcctcc atggtcatcg ggatctggtc cgaggcctgg gctgcttttt
     2521 cgtctgcaag cttctggtcg ttgggtgcgc tcttgaaact ggtggagatc gccaggttgc
     2581 cggaagtaaa ccggcccagg gagaaacctt ttccctccca ggcgtaccgg tcaatatcct
     2641 ggttcaggaa aacgttatgc tggtaggggt ccagggtggc cccgcccgtt atatttatgt
     2701 tcttgaacaa ggtgctgcga aaatacaagg tcagtctcga aagtttgaaa gagtccgcga
     2761 gatagttgta gctccccgac acgccgaatc cgtcgatgat tgtggtcttc ttgatccccg
     2821 cgctggttgt gtcggttttg gagcgggtct tgatctccag gtggttgtcc agttgaaaac
     2881 taaggccgcc aaaagtcccc tccgaaaaag gtccataagt gcttccgtca taataagaga
     2941 cccgttgggt gaccgtggtt tttgccggat cgcccggcgt aaatggcctc ctgatggtgt
     3001 accagtcctt ggccgcaaaa tctggcgtgt agctcaggct taccgtcggc cggataacgt
     3061 ggcggatgcc catcaaactg ctgttcctgc cgaaaccctg gtaggtcccg aagatcgccg
     3121 tcgcgaggct caggctgtag gacatggact cttcctggaa gaccccgcgc tggatagcat
     3181 ccgtgtcaac cttgttgaaa gccgggtcgt agtggcggta aagccggcgg gagtacaccc
     3241 gctcctgcag gctgaccccg ggcgttacct ggaccggccc tttgatcggc agcgccaggc
     3301 tgatgggaat actgttctgc gccccccact ggaaaagcga atcgattcgc cgcagggaca
     3361 ggagggagtc atagaagctg acattccccg tgatcgtact gctgagcccg acgcccagtt
     3421 tctcatacca tttgggcgtc ccgataaaat tgcttttttg cagcggatag agggtcggcg
     3481 ccgtgaagga gatattcggg agcgacacgt tgatctgccg ggtctcgttg ttctggttgt
     3541 ggttggcgct tacgg
//
LOCUS       DATGKE010000249         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1367320, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000249 DATGKE010000000
VERSION     DATGKE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367320"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VKU83_04300"
     CDS             complement(<1..437)
                     /locus_tag="VKU83_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HLI92799.1"
                     /translation="MPTIPSALERQRAIYSRGISGARPSIPLRAEDLRESAKRKMSAT
                     AWAYIDGGAGGEVTIAANAAAFARRAIRPHMMRSKEAANFSCSLFGTSLPFPLLLAPI
                     GAMDLICRGADRMVARAAAETGVPFIFSNQAGTPMEVCAADMGS"
     gene            complement(437..1012)
                     /locus_tag="VKU83_04305"
     CDS             complement(437..1012)
                     /locus_tag="VKU83_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HLI92800.1"
                     /translation="MSTSLKLYMLLLGCKPADRLTEQHDIYFGIGAGLEDLIPQIRQF
                     WPEAGVKLHIDAWRPVTIVDSFALEVLPRKAGGELRERRLFFVNLGGYKPDEFDEFHY
                     KLLIVAPEKGEAIRRAKETAFYRHTGFEGAESHIDDKYGVDVDEVYEIQDILPQAQKE
                     KYALRLTPARNVGPDPLRLGYFQLHKLKNLS"
     gene            1065..1802
                     /locus_tag="VKU83_04310"
     CDS             1065..1802
                     /locus_tag="VKU83_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLI92801.1"
                     /translation="MELSGNILAVDVGGSHIKCIILNRNGEAQTEYRRKATPMEATPA
                     AIIDTLLELANGLPDYDKISIGFPGYVRNGIVFTAPNLGNALWENVQLGQQVSDRLRK
                     PVRLVNDADQLGLGIVSGKGLELAVTLGTGVGTAIMIDGLLLPHLELSHHPISKGRDY
                     DQYIGSRALQDAGKEKWNKRVGKVLRVIKTVFNYDRLYLGGGSCKSLTIPLEDNIILF
                     NNLEGIKGGARLWEMPDGHHITTNYPT"
     gene            1799..>2491
                     /locus_tag="VKU83_04315"
     CDS             1799..>2491
                     /locus_tag="VKU83_04315"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLI92802.1"
                     /translation="MKTKHLVLFALGLLAACKTNPYKSTNRVYKDEAKFFARQLTQEP
                     LPTGVDSVPGAPYWVGTTNFNLRKPNFVIIHHTAQNSCPETLHTFTVARTQVSAHYVI
                     CRDGVVHHMLNDYFRAWQAGVSRWGNVTDVNSISIGIELDNNGFEPFPPAQIGSLLRL
                     LATLKKEYNIPAENFIGHADIAPTRKVDPSSFFPWKELADSGYGLWYRDTAGLTVPDY
                     FSPVAALRIIGYD"
BASE COUNT          576 a          700 c          695 g          520 t
ORIGIN      
        1 gatcccatat cggcggcgca aacttccatg ggcgttcccg cctggttaga gaaaatgaac
       61 ggcactccgg tttccgcggc agcacgtgca accatccggt ccgcaccgcg gcaaatcagg
      121 tccatggcgc cgatcggtgc aagcaacaat ggaaaaggga gcgatgtgcc gaacaaactg
      181 caggagaaat tggccgcttc cttcgaccgc atcatgtgcg ggcggatggc gcggcgtgca
      241 aaggccgccg cattggccgc gatggttact tcgcctcccg ccccgccgtc gatataagcc
      301 caggcggtag cggacatttt tcgcttcgcc gattcccgca ggtcctccgc ccgcaacggg
      361 atggagggcc gggcaccgga aatgccgcgg ctgtaaatgg cgcgttgccg ctcgagagcg
      421 gagggaatgg tgggcatcag gaaaggtttt ttagcttatg caattggaaa tagccaaggc
      481 gcagcgggtc cggtcccaca ttgcgggccg gcgtcaatcg caacgcgtat ttttccttct
      541 gggcttgcgg caggatatcc tggatctcat aaacctcatc gacatctacg ccgtatttgt
      601 cgtcgatatg ggactcggcg ccctcgaagc cggtatgacg gtaaaaggct gtttcttttg
      661 cccgtcggat cgcctcccct ttttcaggcg caacgatcag caatttatag tggaactcgt
      721 cgaattcatc gggtttgtat ccgccgaggt tgacaaagaa gagccggcgt tcccgcagtt
      781 ccccgccggc ctttcgcggc aggacttcaa gcgcaaaact gtccacaatg gttaccggac
      841 gccatgcatc gatatgcagc ttcactcccg cctccggcca gaactgccgg atttgaggga
      901 tcaggtcttc gagaccggcg ccgataccaa aataaatatc gtgctgttcc gtgagcctgt
      961 ccgcaggttt gcatcccaaa agcagcatat ataatttcaa cgaagttgac acgggataaa
     1021 tctagtaaat agtatcttta ttcctttaga aaaccggtaa agaaatggaa ttgtcaggaa
     1081 atatcttggc cgtcgatgtc ggcggttcgc acatcaaatg catcatactg aacaggaacg
     1141 gagaagccca aaccgaatac cggcgtaagg cgacgcccat ggaggcaact cctgccgcca
     1201 tcatcgatac cctgcttgaa ctggccaatg gtttgccgga ttatgacaag atatctatcg
     1261 gctttcccgg ctatgtgcgg aacggcatcg tgtttactgc gcctaacctc ggaaacgcct
     1321 tatgggaaaa tgtccagctc ggtcagcagg tcagtgaccg gctccgcaag ccggtccggc
     1381 tggtcaacga tgccgatcaa ctgggccttg gaattgtcag cggcaaggga ttggagctcg
     1441 cagtaaccct tggaaccggc gtcggaaccg ccattatgat cgacggctta ttgcttcccc
     1501 atctcgaact ctcccaccac cccatcagca aaggccggga ctatgaccag tatatcggaa
     1561 gcagggcgct gcaggatgcc ggcaaggaaa agtggaataa gcgcgtgggc aaggtgctgc
     1621 gggtaatcaa gacagtgttc aactacgacc ggttatacct cggcggcggc agttgcaaat
     1681 cgctgacaat cccgctggag gataacatca ttcttttcaa caacctggag gggatcaagg
     1741 gtggcgcgag gctatgggag atgccggacg ggcatcatat cactaccaat tatcctacat
     1801 gaaaacaaag catctcgtcc tttttgcgct gggcctgttg gcggcctgca aaaccaatcc
     1861 ctacaagagc acaaaccggg tgtacaaaga cgaagcgaag ttcttcgccc gtcagttgac
     1921 ccaggaaccg ctgccgaccg gcgtcgattc cgttcccgga gccccctatt gggtaggcac
     1981 caccaatttc aatttacgca agccgaactt cgtgatcatc caccatacgg cgcagaacag
     2041 ctgtcccgag acgctccata ctttcacggt cgctcgaacg caagtgagcg cgcattacgt
     2101 gatctgccgg gatggcgtcg tccaccacat gctgaacgat tatttccggg catggcaggc
     2161 aggcgtgagc aggtggggaa atgtcacgga cgtcaattcg atctccatag ggatcgagtt
     2221 agacaacaac ggcttcgagc cgttcccgcc ggcgcagatc ggcagcctgc tccggttact
     2281 cgcaacgctc aagaaagagt ataatatccc cgcggaaaat tttatcggcc atgccgatat
     2341 tgcgccgaca cggaaagtgg atccgagctc cttttttcca tggaaggagc tggcggattc
     2401 gggatatggc ttgtggtacc gcgacaccgc cgggctcacg gtgccggatt atttcagtcc
     2461 cgtagcagcg ctccggatta tcggatacga t
//
LOCUS       DATGKE010000250         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_354212, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000250 DATGKE010000000
VERSION     DATGKE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354212"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2110
                     /locus_tag="VKU83_04320"
     CDS             <1..2110
                     /locus_tag="VKU83_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLI92803.1"
                     /translation="ATASPDLPGDKAGVIQPAFQGGACDGFVAQISGDGTTLKKVTYL
                     GTSDNEAIYGLQFDKYGFPYVMGTTNATWPVINAAYSVSGARQFVSKLRPDLSGYVYS
                     TTFGTPSPGGHGLPNISPVAFLVDRCQNVYVSGWGGWILGNETDPYGLAGTSGMPVTS
                     DAIKPVSDNRDFYFIVIKKNASALLYGTFYGQNDNSKSISEHVDGGTSRYDQNGVIYQ
                     GICANCDGQSSSPFPTTVGVWGPVNASTGCNEAALKIAFNFAGVAAGLRVNANGRLWD
                     TSGCTPLTADLLDTIRNAKSYIWNFGDGGPDTATTTYEVQHTYNNPGRYRIMLVAIDS
                     NSCNVADTTYRQVIARTNKAPVDFAFVKDPNLPCTALTYDFTNLSTAPPGLPFGPASF
                     VWEFGDSTNPVQTGPGPQTHTYLNPGTYIVNLIMDDTAYCNYPDTAIKTLRVSPLAKA
                     QFITPATGCAPDSAVFNNTSLGGIQFTWNFGDGSPPDSDPNPVHLYANPGTYVVTLLE
                     VDTTTCNQRDSTQFTIVISGKPTAGFTYTPETPVANTPLVFTNGSVGAVRFKWLFGDG
                     DSTLKTTMDTVVHLYNQTDTFNACLLATNAAGCTDTICHPVEALINPLLDVPNAFTPG
                     RFGENSIIRVMGFGITHMDFRIYNRWGKMVFQSTDPNIGWDGYYQGQLQPMDVYAYTL
                     EADFSNGKHVTKKGDITLIR"
BASE COUNT          498 a          638 c          654 g          473 t
ORIGIN      
        1 cgctacggcg agcccggacc ttccgggcga taaggcgggg gtcattcagc ctgcgtttca
       61 ggggggcgcc tgtgatggct tcgtggccca aatctccggg gacgggacaa cgctcaaaaa
      121 ggtaacctat ctcgggacgt ccgacaatga agccatttat ggcctgcaat tcgacaaata
      181 cgggtttccc tacgtgatgg gcacgaccaa tgcaacgtgg cctgtgatca atgcagccta
      241 ttcggtatcc ggcgcccggc aattcgtgag caagctccgg cctgacctgt cgggctatgt
      301 gtactctacg accttcggca ccccctcccc ggggggccat ggactgccaa acatttctcc
      361 ggtggctttc ctggtcgacc gctgccagaa cgtctacgtc agcggctggg gcggatggat
      421 ccttggcaac gaaacggacc cctacgggct ggcgggaact tcaggcatgc cggtgacgag
      481 cgatgcgatc aagccggtca gcgacaaccg ggacttttac tttatcgtca ttaagaagaa
      541 tgcgtcggcc cttttgtatg gcacgtttta cggccagaac gacaacagca aaagcatcag
      601 cgaacacgtc gacggcggga cgagcaggta tgaccagaac ggcgtcatct accagggcat
      661 ctgcgccaat tgcgacgggc agtcgagcag cccgtttccg accacggtgg gagtgtgggg
      721 cccggtcaat gcgtccaccg gctgcaatga ggcagcgctg aagatcgcat tcaatttcgc
      781 gggcgttgcg gcgggtttaa gggtcaatgc gaatgggcgc ctatgggata cctcgggctg
      841 cacgccgctg accgcggacc tgctggacac tatccggaac gcgaagtcct atatatggaa
      901 tttcggcgac ggcggcccgg ataccgcaac cacgacctac gaggtgcagc atacctataa
      961 caatccggga aggtatagga tcatgctggt ggcaatcgac tccaatagct gcaatgtcgc
     1021 cgatacgacc tatcgtcagg tcattgcacg gacgaacaag gctccggtgg attttgcctt
     1081 tgtcaaagat cccaatctgc cttgcacggc gctgacttat gatttcacca atttgtcgac
     1141 ggctccgccg ggtttgccgt tcgggcctgc ttccttcgtg tgggaattcg gcgattccac
     1201 gaatcccgtc cagaccgggc ctgggcctca aacgcacacg tacctgaatc caggcacgta
     1261 tatcgtcaac ctgatcatgg acgacaccgc ctattgcaat tatcccgata cggccatcaa
     1321 gaccctgcgg gtgtcaccgc tggcgaaggc gcaattcatt acgccggcaa cgggctgtgc
     1381 gccggactct gccgttttca acaatacttc cctcggcggc atccaattca cgtggaattt
     1441 cggcgacggc tcgccgcccg actccgatcc gaatccggtc catctgtatg ccaatccggg
     1501 aacctacgtc gtgaccctgc tcgaagtcga cacaacgact tgcaaccaac gggactcgac
     1561 acagtttacg attgtgatca gcggtaaacc cacggccggg tttacttata cgcccgagac
     1621 gccggtggcg aatacgccgc ttgtgtttac gaacgggtcg gtgggagcgg tccggttcaa
     1681 gtggttgttc ggcgacgggg attccaccct gaagacgacc atggatacgg tggtgcatct
     1741 ttataatcag acagacactt tcaacgcctg cctgctggcg acgaatgccg cgggctgcac
     1801 ggatacgatc tgccatccgg tcgaagccct gatcaatccg ttgctcgacg tgcccaatgc
     1861 ctttacaccg gggcggttcg gggagaattc catcatccgg gtgatgggct ttggcattac
     1921 gcatatggat ttcaggatat acaaccggtg gggcaagatg gtgttccagt cgacggaccc
     1981 caatatcggc tgggatgggt attaccaggg acaattgcag ccgatggatg tgtacgctta
     2041 tacgctcgag gcggattttt cgaacggtaa gcacgtaacc aaaaaaggag atataacttt
     2101 gattcgttaa cggaagctaa aaagacaacc aaaaagatca gacggtcgtt actaaaaaaa
     2161 tgcgtggctg gagaaatatg atccttgtgg gctgctgtat cgccggcagc attgcggaag
     2221 cggccggcca ggacctgcat ttttcgcagt ggttcaattc ccc
//
LOCUS       DATGKE010000251         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_331144, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000251 DATGKE010000000
VERSION     DATGKE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331144"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VKU83_04325"
     CDS             <1..652
                     /locus_tag="VKU83_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLI92804.1"
                     /translation="INHGIDSFNMQEDDLKIRETDYKAQTSTVLMIDISHSMILYGED
                     RITPAKKVAMALSELITTKYPKDTLDIVVFGNDAWPVEIKDLPFLQVGPYHTNTVAGL
                     ELAMDILRRRRNANKQIFMITDGKPTCLKVGKRYYKNSFGLDRKITSRCLNLAAQCKK
                     LKIPITTFMIASDPYLQRFVNEFTETNNGKAFFASLDRLGSFIFRDFESGKRKTVY"
     gene            677..2206
                     /locus_tag="VKU83_04330"
     CDS             677..2206
                     /locus_tag="VKU83_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLI92805.1"
                     /translation="MDITKIKTLGELKDAGYRKKSVKEEIRVNLIRKLKNKETTFPGI
                     LGYEESVIPDTERALLSRHNILFLGLRGQAKTRMARQMVELLDEYIPYVTGSEVNDNP
                     FEPISKFARELVAAEGDDTPISWLHRSDRYGEKLATPDVSVADLIGDIDPIKAANLRL
                     NFADEQVLHFGIIPKSNRGIFVINEIPDLQARIQVSLFNILEEGDIQIRGFKLRMPLD
                     MLFVFTANPEDYTNRGSIVTPLKDRIESQILTHYPKNLDTALAITEQEADIHEEQKGI
                     VMVSDLVKRLIEQVSFEARTSEYVDKKSGVSARLTIAAFENAVSAAERRAILNNERQT
                     QVWMSDLIGIVPSITGKIELVYEGEQEGPYQVAFNLLEKAFRSQFVNYFPNPDQLKKR
                     KAETRDENPFRAITHWFDKGNHLNLFFNTPDAEKVQQLYKVDGLHSLVKRYFKHANER
                     EAALLMEFVLHGLSAYSLISKKVVEGKIEFKDLIGSMVNLGSSGSSFGNEEEEEGEDF
                     N"
     gene            complement(2203..3258)
                     /gene="murB"
                     /locus_tag="VKU83_04335"
     CDS             complement(2203..3258)
                     /gene="murB"
                     /locus_tag="VKU83_04335"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HLI92806.1"
                     /translation="MGWKEDISLRPYNTFGIEASARHFARFSDADQLEALLRSGPFAH
                     QGRPASLRRMSPGSLLVLGGGSNILFTRDPEAWVLKNEIAGIRLLREDNRYVYVQVGA
                     GEVWHDFVQHCLKRDWAGVENLSLIPGSVGAAPMQNIGAYGVELKEFFHELEAWDLQT
                     GKVRIFTVNDCRFGYRESVFKQEYKERFIILNVTFRLNHKPVFHTGYGAIREELEKMQ
                     VKQLSIRAISEAVVRIRSAKLPDPARIGNAGSFFKNPTIPNAQFMSLKARFPDIVAFP
                     DPVHPVTKLAAGWMIEQCGWKGFRRGDAGCHERQALVLVNYGKATGREILALSEEILR
                     SVHQKFGITLEREVNIV"
BASE COUNT          841 a          975 c          935 g          720 t
ORIGIN      
        1 gatcaatcac ggcatcgatt ctttcaatat gcaggaggac gacctgaaga tccgcgaaac
       61 cgactacaag gcccaaacct cgaccgtact gatgatcgac atcagccatt cgatgatcct
      121 ctatggcgaa gaccgtatca cgccggcaaa aaaggtcgcc atggcgctca gcgagctgat
      181 cacaaccaaa tatccgaagg acacgctcga cattgtggtc tttggcaacg acgcctggcc
      241 cgtcgagatc aaggacctcc ccttcctgca ggtggggccc taccacacga acaccgtcgc
      301 cgggctcgag cttgccatgg atatcctccg gcgacgccgg aatgccaaca aacaaatctt
      361 catgatcacc gatggcaagc cgacttgcct gaaggtggga aaacgatatt acaagaatag
      421 cttcgggctc gaccgcaaga tcacgagccg ctgcctgaat ctcgcggccc agtgcaagaa
      481 attgaagatc ccgatcacga cgttcatgat cgccagcgat ccgtatctcc agcggttcgt
      541 caacgagttc accgaaacga acaatggaaa agcttttttt gcctctctcg acaggctggg
      601 cagtttcatc ttccgggact ttgaatccgg gaagcgcaaa acggtttatt gagtaaatcg
      661 aaacattata acgcaaatgg acattacaaa gatcaagacg ctgggagaat tgaaagatgc
      721 cggatatcgt aagaaatccg tgaaggagga gattcgtgtc aacctgatcc gcaagctgaa
      781 gaacaaagaa acgacattcc ctggaatcct tggctatgaa gaatcggtta tcccggatac
      841 cgagagggcc ctgttatccc gacataatat tctttttctc ggacttcgcg gtcaggcgaa
      901 gacgaggatg gccaggcaga tggtcgagct tctcgacgag tatattcctt atgtcaccgg
      961 cagcgaggtc aacgataatc ctttcgagcc gatatccaaa ttcgcccgcg agctggtggc
     1021 tgcagaaggg gatgacacgc ccatcagctg gctgcaccgc agcgaccggt atggcgagaa
     1081 gctggctacg cccgacgtaa gcgtggcaga ccttatcggg gacatcgatc cgatcaaggc
     1141 tgcgaatctg cggcttaact tcgccgacga gcaggtcctc cattttggga tcatcccgaa
     1201 gagcaaccgg gggatctttg tgatcaacga gatcccggac ctccaggcgc ggatacaggt
     1261 ttcacttttt aatatacttg aggaaggcga tatccagatc aggggtttta agctccggat
     1321 gccgctcgac atgttgtttg tctttaccgc caaccctgag gactatacca accgcggctc
     1381 gatcgttact ccgcttaaag accggatcga aagccagatc ctcacgcact atcccaagaa
     1441 cctggacacg gccctggcca tcaccgaaca ggaagccgat atccacgagg aacaaaaagg
     1501 gatcgtgatg gtaagcgacc tcgtcaaacg actcatcgaa caagtctcgt tcgaggcccg
     1561 caccagcgag tatgtcgata agaaaagcgg ggtttcggcg cggctgacga ttgccgcttt
     1621 cgagaatgcc gtgagcgccg ccgagcggcg ggccatcctc aacaatgaac ggcagaccca
     1681 ggtctggatg agcgatctca tcggcatcgt cccgtccatc accggcaaga tcgaattggt
     1741 ttacgagggt gagcaggaag gtccttacca ggtagcattc aacctgctcg aaaaggcttt
     1801 tcgctcgcag ttcgtcaact actttccaaa ccccgatcaa ctgaagaagc ggaaggccga
     1861 aacccgcgat gagaatcctt tccgggccat tacgcactgg ttcgataaag ggaatcacct
     1921 gaaccttttc ttcaacaccc cggacgcaga aaaggtccag caactgtaca aagtggatgg
     1981 cttgcattcg cttgtaaaaa ggtatttcaa acacgccaac gagcgggaag cagccctgct
     2041 tatggaattt gtgctacatg gtctctcggc ctattcgttg attagcaaaa aggtcgtcga
     2101 gggcaagatc gagttcaaag acctgatcgg ctcaatggtt aacctcggct cgtcgggcag
     2161 cagcttcggc aatgaagaag aggaagaggg ggaagatttc aattagacga tattgacctc
     2221 acgttcgagg gtaatcccga acttctggtg cacactccgg aggatttcct cgctcagggc
     2281 aaggatctcg cggccggtcg cctttccgta attgacgaga accagcgcct ggcgctcgtg
     2341 acagccggcg tctccccggc gaaagccttt ccagccgcat tgctcgatca tccagccggc
     2401 cgccagtttg gtaaccgggt ggacggggtc cggaaaggca acgatgtcgg ggaatcttgc
     2461 cttgagggac atgaactgcg cattggggat cgtcggattc ttaaaaaaac ttccggcatt
     2521 tccgatcctg gccggatccg gcagcttggc cgaccggata cgaacgaccg cttccgagat
     2581 cgcccggatg ctcagttgct tcacctgcat cttctccagt tcctcccgga ttgccccata
     2641 accggtatga aaaaccggct tatggtttag gcggaacgtg acgttcagga taataaaacg
     2701 ttccttgtac tcctgcttga agacgctttc gcgataaccg aaccggcaat cgttgacggt
     2761 aaagatcctc accttgccgg tctgaaggtc ccaggcttcc agttcgtgga agaattcttt
     2821 cagttccacg ccgtaggcgc cgatgttctg catcggggcg gcgccgacgc tgccggggat
     2881 cagcgagagg ttttcgacac cggcccaatc ccgcttcagg cagtgctgca cgaaatcatg
     2941 ccagacctcg ccggctccga cttgtacata tacataccgg ttatcctccc gcagaagccg
     3001 gatgcccgca atctcgttct tcagtaccca ggcctcgggg tcccgcgtga acaggatatt
     3061 gctgccgccg ccgaggacaa gcagcgagcc gggcgacatt cgccggaggg aagccggccg
     3121 gccttgatgc gcgaaagggc ccgaacgaag aagcgcttcc agttggtcgg catcggagaa
     3181 tcgcgcaaaa tgccgggcgc tggcttcgat cccgaaagta ttgtatggtc gtaatgaaat
     3241 atcttctttc caccccatga cacaatgata cgttttgact gcaatgtagg acgacttttt
     3301 tgagaaaaag gcataacttg gtaagacaaa atgtcgatta tgccatcacg ccgtcgcttc
     3361 ctgcgggcac tgaccggttc tgccctcgtg gtttccgccg ccccaggcac cctggccggc
     3421 ggatgtgcca gcgccgcttc ttcccggtac gacggtcccg tgctccgggt g
//
LOCUS       DATGKE010000252         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1340747, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000252 DATGKE010000000
VERSION     DATGKE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340747"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VKU83_04340"
     CDS             <1..624
                     /locus_tag="VKU83_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92807.1"
                     /translation="TLADLAHVSYVSDANCFDFGVGLDGDVSGGPSVYFKLGYGRIFP
                     FGRWQVQPTVDFYWAMDRTSRLGILNNYDTTINILGYSADPYFTQIYTDDYGNEDVYT
                     YTAGDLDVNYTRNSFLAVPKVLVGTILWRHLYVGIEAGWLQQLAQSNVIKLVQYDAYG
                     SGASNVVGKVHLKTNGSLTGPELAFNLGWCIPYHPRPQRNYYYNNNE"
     gene            complement(637..3678)
                     /locus_tag="VKU83_04345"
     CDS             complement(637..3678)
                     /locus_tag="VKU83_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLI92808.1"
                     /translation="MRKPVLLALLLTIGITRSFGQHRIAPSADAWVDSVFKTLTPDEK
                     IAQLMVIRLSGIDPASHRPVFYDSIVEDAVRRYNVGGVCTFQGEPVAQAQHINHLQAI
                     ARTPILFCMDAENGLGMRMDSVTALPRQMMLGATADTGLIYRYGLLVGAQCKRLGIQV
                     DFAPVVDINNNPANPVINDRSFGEDRDKVAAFGIQYMKGLQASGIMACAKHFPGHGDV
                     SVDSHLDLPVINKDRRQLDSLELYPFRQLIKAGVPAIMIAHLYIPAIDKRPNRATSLS
                     YEAVTKLLRKQLHFRGISFTDALEMQGVAKYFPDGDAAVESLIAGNDMLSLPGDVGQS
                     IARIKMALKKKKLHWKDIDARVKTVLYAKYQYGLASWHPVNTAHLVPELNAEVEEMRR
                     EIAQDALTLLRNDDSAVFPLLAATVGPQALVSAQPIRKKRIAYLGLGLTEENSFARRM
                     REDYSADVYLFDYNLDSAKAAAAFELLSGRYDAIVIGLHNYARYPANNFLISGPAVRL
                     LHQLQDRERCVTLAFGNPYALSNTCQARVLVACYEDDSTTQEVAADLLNGKFTARGHL
                     PVTVCSLYRAGAGITGPYRALPMANPLSVGMDPATLARIDAICNEAIAQQAAPGCVVL
                     VARDGKLAYEKAFGYMTYDRKEPVYTETLYDMASCTKICATTMAVMKLYDEGRLDLQK
                     TLGDYLPWVRGSDKAPLTLWDILLHQAGLVPDVVFYKEVVDSTKDENPMPGIFSNRPD
                     SLHGVRVAQDLYLRNDWRDTMDLRIVQSKLGPRKYVYSDNDFILLGEIVEAITGMSLD
                     EYVRKTFYDPLGMTSTGFLPRKRFPLGRIAPTAAEPIFRHQLLRGDVHDPGSAMFGGV
                     AGHAGLFSDAYDLAILAQMLLDGGTFNGHTFLKKSTIDYFTAYQTDLSRRALGFDKPE
                     KDNATLAKPYPCLSASPETFGHTGFTGTCFWVDPKYDLIFIFLSNRVNPVENNPRLSA
                     LDIRGRIQETVYQAMGIDEGASTRAPDPKGKRRRRGKHHTA"
     gene            3712..>3967
                     /locus_tag="VKU83_04350"
     CDS             3712..>3967
                     /locus_tag="VKU83_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92809.1"
                     /translation="MPDIIQLLPDNIANQIAAGEVIQRPASAVKELLENAVDSGATDI
                     RLFVQDSGKALIRVVDNGKGMSETDARMAFERHATSKIRQI"
BASE COUNT          827 a         1152 c         1129 g          859 t
ORIGIN      
        1 acccttgccg acctggcgca tgtatcctac gtcagcgatg cgaattgctt cgacttcggc
       61 gtcggccttg acggggatgt gagcggcggg cccagcgtct acttcaaatt gggctatggc
      121 cgcatcttcc cgttcggccg ctggcaagta cagcccaccg tagattttta ctgggcgatg
      181 gacaggacca gccggctggg catcctcaat aactacgata cgaccatcaa catcctgggc
      241 tacagcgccg atccgtattt cacccagatt tacaccgacg attatggcaa cgaagacgtg
      301 tacacctaca cggccggcga cctggacgtg aattatacgc gcaacagctt cctggctgtt
      361 cccaaggtcc tggtgggcac gatcctatgg cgccatctct atgtcggcat tgaggccgga
      421 tggctccaac agctggcgca aagcaatgtc atcaagcttg tccagtacga tgcctatgga
      481 agcggagctt cgaacgtggt cggcaaggtc cacctgaaaa cgaacggttc cctcaccggt
      541 cctgaattag cctttaacct cggctggtgc atcccttacc accctcgtcc gcaaaggaat
      601 tattattata ataataatga atgatccggc ttaatttcag gccgtatggt gtttcccgcg
      661 ccggcgcctt ttccctttgg gatcgggcgc cctggtgctg gcgccttcat cgatgcccat
      721 tgcctggtat acggtttcct ggatgcggcc gcggatatcc aacgccgata gccgcgggtt
      781 attttcgacc gggttaacgc ggttcgatag aaaaataaaa atcagatcgt atttcgggtc
      841 cacccagaag catgttccgg taaatccggt atggccgaag gtctcgggcg aggccgagag
      901 gcaggggtag ggcttcgcaa gcgttgcatt gtccttttcc ggtttatcaa accccagggc
      961 ccggcggctg agatcggttt gatatgccgt aaaatagtcg atggtagatt ttttcaggaa
     1021 agtatggccg ttgaaggtgc ccccatccag gagcatttgc gccaggatag ccagatcata
     1081 ggcatcactg aataatcccg cgtggcccgc caccccgccg aacatcgcag agcccgggtc
     1141 gtgaacgtcc ccgcggagca actgatgccg gaagatcggc tccgccgcgg tgggcgctat
     1201 ccgccccaat gggaaacgct ttctggggag aaatcccgtg gacgtcatcc ccaacggatc
     1261 atagaaagtc ttcctgacgt actcatcgag gctcataccg gtgatggcct ccacgatctc
     1321 gcctaataaa atgaagtcgt tatcgctata gacatatttt ctcggcccca gcttgctttg
     1381 cacgatccgg agatccatcg tatcccgcca atcattgcgc aggtagagat cctgcgccac
     1441 gcggacgcca tgaaggctgt cggggcggtt gctgaagatg cccggcatcg ggttctcgtc
     1501 cttggtgctg tccaccactt ccttgtagaa aaccacatcg ggcaccagtc cggcctggtg
     1561 cagaaggata tcccagagcg tcagcggagc cttgtcggag cctcgtaccc aaggcaaata
     1621 gtctcccagc gtcttttgca ggtccagccg gccctcgtcg tatagcttca tgaccgccat
     1681 ggtcgtcgca cagatcttcg tgcaggaagc catatcgtac agggtctctg tatacacggg
     1741 ttccttccgg tcataggtca tatagccaaa cgccttttca taggccagct ttccatcacg
     1801 ggcgaccagc actacacagc caggcgcggc ctgttgcgca atcgcctcat tgcagatcgc
     1861 gtctatcctg gccagcgtcg ccgggtccat gccgaccgaa agcggatttg ccatcggcag
     1921 cgcgcggtat ggcccggtga ttccggcgcc cgcgcggtag agactacaga ccgtcaccgg
     1981 cagatggccg cgcgcggtga acttaccgtt cagcaggtcg gccgctactt cctgcgtcgt
     2041 agaatcgtct tcataacagg caacgagcac cctggcttga caagtgttgc tgagagcgta
     2101 gggattaccg aatgccaggg tcacgcaacg ttccctgtcc tgtaactggt ggagcagccg
     2161 gacggccgga ccgctgatca ggaaattatt tgccggatac cgcgcgtagt tatgcagccc
     2221 gatcacgatg gcgtcgtaac ggccgctcaa caactcgaac gcggccgcag ccttggcgct
     2281 gtccaggttg taatcaaaca ggtagacatc cgcgctatag tcctcgcgca tccgccgcgc
     2341 gaacgaattt tcctccgtca agcccagtcc gagatacgcg atccgctttt tcctgatggg
     2401 ctgcgcggaa accagcgctt gcggtccaac ggtcgcggcc agcaggggga agaccgccga
     2461 gtcgtcattg cgcagcaggg tcagggcatc ctgcgcgatc tcgcgccgca tctcttctac
     2521 ctcggcattg agctccggga caagatgagc cgtattgacg ggatgccagc tggcgaggcc
     2581 atactggtat ttggcgtaca gaacagtctt gacgcgcgca tcgatgtcct tccagtgaag
     2641 cttctttttt ttcagtgcca tctttatcct ggcgatgctc tggccgacat ctccgggcag
     2701 gctcagcatg tcgtttcctg ctatcagcga ctcgaccgcc gcgtcgccat cggggaaata
     2761 tttggcgacc ccctgcatct caagggcgtc cgtaaagctg atgccccgga aatgcaattg
     2821 ctttctgagc agcttcgtca ctgcttcata ggacaacgag gtcgcgcggt ttggcctctt
     2881 gtcgattgcc ggaatgtaca aatgggcgat catgatcgcg ggtacgccgg ccttgatcag
     2941 ttgccggaat ggatagagct ccagggaatc cagctggcgt ctgtccttgt tgatcaccgg
     3001 aagatccagg tgcgagtcca cgctcacgtc tccatgaccg ggaaagtgct tcgcgcaggc
     3061 catgatcccc gatgcctgca ggcccttcat atattgaatg ccgaatgcgg cgaccttatc
     3121 gcggtcctca ccgaaagacc ggtcattgat gacggggttc gcggggttgt tgttgatatc
     3181 cacgacaggc gcgaaatcca cctggatgcc tagccgctta cactgcgcgc cgaccagcag
     3241 accgtagcgg tagatcagac cggtatcggc ggtcgcaccg agcatcatct ggcgcggcaa
     3301 agccgttacg ctgtccatgc gcattcccag tccgttctcc gcatccatgc aaaacaggat
     3361 cggcgtcctg gcgatggcct gcaagtggtt gatatgttgc gcctgggcta ccggctctcc
     3421 ctgaaaggtg cacacgccgc cgacattgta tctcctgact gcatcctcga cgatcgagtc
     3481 gtaaaacacc ggccggtggc ttgcgggatc gatgccggag aggcgaataa ccatgagctg
     3541 ggcaatcttt tcatcaggag tgagggtttt gaagacacta tctacccagg cgtcggcgga
     3601 aggagcgata cgatgttggc caaagctgcg ggtgatacca atcgtcagga gcagcgccaa
     3661 caggacgggt tttctcattt aggcgtaaat tgcacacaaa ataagtcaga tttgccagac
     3721 attattcaat tattgcccga taacattgca aatcagatcg cagcgggcga ggtgatccag
     3781 cggcctgcca gcgcggtaaa ggaactgctc gagaatgcgg tggattcggg cgcgaccgac
     3841 atcaggctct ttgttcagga ctccggcaag gcgttgatcc gtgtggtcga caatggcaaa
     3901 gggatgagtg aaacggatgc caggatggcc ttcgagcgtc atgcgacgtc caagatccgg
     3961 cagatcg
//
LOCUS       DATGKE010000253         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_815957, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000253 DATGKE010000000
VERSION     DATGKE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815957"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VKU83_04355"
     CDS             complement(<1..750)
                     /locus_tag="VKU83_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HLI92810.1"
                     /translation="MQNGQFIRKVLMIATAACLGIAGCVQSYISPYKSPPTGYLVVEG
                     FISGNGPTRYTLSRTIPLPGDSVIPKVTGATLQVEGTDNSVYPLTEQGNGEYGADSLP
                     LSLNVKYRLRVGTPNGKSYLSAYVPYKPTPPIDSVNWAQNSAGVNIYVNTHDPAGATR
                     YYEWSYDQTYQYTAAEASSFIYDAATNSVDPRPDSLQIFTCWKDEPSTRIVVGTSSGL
                     AQDEIYRLPLVQILPNTQPLSVLYTLLVTQYS"
     gene            complement(750..>3137)
                     /locus_tag="VKU83_04360"
     CDS             complement(750..>3137)
                     /locus_tag="VKU83_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92811.1"
                     /translation="IHDYLADLGKPAVATVENKLYVIGDKADAHRPGKVNLAGYTLDA
                     ATGEPIVGASVFVEDPRIGVSSDQYGYYSISLPRGRHVLNIQSIGMRDTRRLIMLYGD
                     GKMNIELRTQVMTLKRVVVSAEKMNNIKGTEMGVQKIDIRTIKQVPVVFGEADILRVV
                     TMLPGVQTVGESSTGLNVRGGSADQNLVLFNDATIYNSAHFFGFFSAFNPEVVKDVEL
                     YKSSVPARYGGRLSSVLDIDGREGNKRNFTGSAGIGLLTSRLNIEGPIVKDKTSFILG
                     GRTTYADWLLQELPAQYRNSRASFYDVNLTVSHEINKKNNLYLTGYLSEDHFNLNNDT
                     TYAYGNRNINVKWKHIFSNKWNSVVSAGYDRYLYSITATNNPVNAFNLGFNINQLYLK
                     ANFNYYLNSNHTLEFGLNTLRYKLHPGYYLPVGSKSLVTPDTLQAEQAQESALYLSDH
                     YTITSALSLEAGLRWSVYNYLGPETVNNYAPGQPLTVQTQTGATDYANGKIIKTYGGP
                     EYRLSARLVLDENFSLKAGYNTNRQYISMLSNTTAMAPTDIWKLSDPNIRPQYGDQLS
                     LGAYRNFKSNTIETSVEVYYKRIQDYLDYKPGAQLIMNPHIETDVLETHGKAYGVELL
                     IRKSTGKLNGWISYTYSRILLRQNNPNEGPLINNGNWYPADYDKPHDAVVVGNYRFNH
                     RFSVSVNATYSTGRPITLPIGVYYYAGSLRTLYADRNAYRIPDYFRTDFSMNIDGNHR
                     IHQKTHNSWTVGVYNLTGRHNPYSVYYVSQNGAVNGYQLSIFGSAIPFVNYNIRF"
BASE COUNT          676 a          802 c          907 g          752 t
ORIGIN      
        1 cgaatactgc gttacaagga gagtatacaa tacggaaagc ggctgcgtgt tcggcaggat
       61 ctggacgagc ggtaaccgat aaatctcatc ctgcgccagg cccgaagacg tgccgaccac
      121 gatgcgggtt gatggctcgt ctttccagca agtgaatatc tgcagggaat ccggtcgggg
      181 atcaacggaa ttagtagcag cgtcatagat gaaactcgat gcctccgccg ccgtatattg
      241 gtaagtctgg tcatatgacc attcgtaata ccgggtggcg ccggccgggt cgtgcgtgtt
      301 gacatagatg ttaacgccgg ccgaattctg ggcccaattg acgctgtcaa tcggcggggt
      361 tggcttataa ggtacatacg ccgacaggta agacttgccg ttcggagtgc cgacacgcag
      421 gcggtatttg acattcaggc ttaacggcag ggagtccgcg ccatattccc cattcccttg
      481 ttcggtaagc ggataaacgg aattgtccgt tccctccacc tggagtgtcg cgccggtaac
      541 tttcgggatg acagaatcgc ccggcagcgg aatggtgcgc gaaagcgtat agcgggtagg
      601 cccgttgccg gaaataaagc cctcgacgac gaggtagccg gtcggcggcg atttgtacgg
      661 gctgatatag gactgaacgc aaccggcaat tcccaggcag gccgccgtcg caatcatcag
      721 gacctttcga ataaattgac cgttttgcat cagaaccgga tattgtagtt aacaaaaggg
      781 atggcgctgc cgaagatcga aagctggtat ccattgactg ccccgttctg cgaaacataa
      841 tacaccgagt aggggttgtg ccggccagtc agattgtaca cgccgactgt ccatgagtta
      901 tgcgtctttt gatggatcct gtggttgccg tcgatgttca tcgagaaatc ggtgcggaaa
      961 taatccggga tccggtaagc attccggtcg gcatagagcg ttcgcaggga accggcataa
     1021 taatagacgc cgatgggcag cgtgatcggc cggcccgtgc tgtaagtggc attcaccgat
     1081 acgctgaagc ggtggttgaa ccggtagttg ccaaccacaa cggcgtcatg gggcttgtca
     1141 taatccgctg gataccaatt tccgttgttg atcagcggcc cctcattggg attgttttgg
     1201 cgcaggagga tgcgggaata agtgtagctg atccagccgt tcagctttcc ggtcgatttc
     1261 ctgatcagca gctccacgcc gtaagcctta ccgtgggtct ccaatacgtc ggtctcgata
     1321 tgcgggttca tgatcagttg ggcgccgggc ttataatcaa ggtagtcctg tatccgctta
     1381 tagtacactt caacggaggt ctcgatggtg ttcgacttga agttccggta ggcgcccagt
     1441 gacaactggt ctccgtactg tggccggata ttcgggtcgc tcagcttcca gatatccgtc
     1501 ggcgccatcg ccgtcgtatt ggacagcatg ctgatatact gacggttagt gttgtatccg
     1561 gccttcaggg agaaattctc gtcaaggacc agcctggcgg acaggcggta ctccgggccg
     1621 ccataggttt tgattatttt accgttggca tagtccgtcg cgccggtctg cgtctgcacg
     1681 gtcagtggct ggcccggggc ataattgttc accgtctccg gtccaagata attgtagacc
     1741 gaccaacgaa gcccggcttc gagcgaaagc gcgctggtaa tggtatagtg atcgcttaga
     1801 tataaggcgc tctcctgcgc ctgctcggct tgcagggtat cgggagtaac cagcgacttg
     1861 ctgcccaccg gcaggtagta acccgggtga agtttataac gaagcgtatt gaggccaaat
     1921 tccagggtgt ggttcgaatt caggtaatag ttgaaattgg ccttcagata gagctggttg
     1981 atgttgaagc ccaggttaaa agcattcacc gggttgttgg tcgcagtgat cgagtacaga
     2041 tagcggtcgt agcctgcgga aaccacgctg ttccatttgt tgctgaagat gtgcttccat
     2101 ttcacgttga tattcctgtt tccgtaagca taggtcgtgt cattgttgag gttgaaatga
     2161 tcctcgctca ggtaaccggt aagataaagg ttgttcttct tgttgatctc atggctgacc
     2221 gtcaggttga cgtcatagaa tgaggcccgg ctgttgcggt attgcgcagg aagttcctgt
     2281 aacagccagt cggcataggt cgtcctgcca ccgaggataa aactggtctt gtccttaacg
     2341 atcggcccct ctatgttcag ccggctggtt aggagaccga tgcctgcgga cccggtgaaa
     2401 ttacgcttat tcccttcccg gccgtcaatg tcaagaacgg atgacaggcg gccgccgtag
     2461 cgcgcgggaa cgctgctttt gtacagttcc acgtctttca ccacttccgg gttgaaggcg
     2521 gaaaagaagc cgaagaaatg cgcagaattg tagatggtgg catcgttgaa caagacgagg
     2581 ttctgatcgg cggacccgcc gcggacgttc aggccggtgc tcgattcgcc gacagtctgg
     2641 acaccaggca gcatggttac cacccgcagg atatccgcct cgccgaatac gacagggacc
     2701 tgtttgatcg tcctgatatc gatcttctgg acacccatct ctgtcccctt aatattgttc
     2761 attttttcag ccgataccac caccctcttg agcgtcatga cctgcgtgcg gagctcgatg
     2821 ttcatttttc cgtccccata caacatgatg agccggcggg tatctctcat gccgatgctt
     2881 tgtatattca ggacatggcg gcctcggggc agggagatgg agtaatagcc atattgatca
     2941 gaagacacgc caatcctggg gtcctcgaca aagacggaag ccccgacgat cggttccccg
     3001 gtcgccgcat cgagcgtata gccggcaagg ttaacctttc ccggccggtg cgcatcggct
     3061 ttatcaccga taacgtagag tttgttttcc accgtagcca cggccggttt accgagatcc
     3121 gccaggtaat catgtat
//
LOCUS       DATGKE010000254         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_431926, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000254 DATGKE010000000
VERSION     DATGKE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431926"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..554
                     /locus_tag="VKU83_04365"
     CDS             69..554
                     /locus_tag="VKU83_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLI92812.1"
                     /translation="MLVRHAKSSWDSAHTPDIERPLSDRGKKDAPLMAKRLRKAGIHP
                     DRLVSSPARRARHTAELFARELDVDEKEIDIRTQLYHAQPATFQQVIAGLNDGDQTVA
                     LFSHNPGITDFVNTLGSVHLDNMPTCAVFAVKKRCEKWAEFLSGNPEFLFFDYPKSAA
                     D"
     gene            complement(530..1399)
                     /locus_tag="VKU83_04370"
     CDS             complement(530..1399)
                     /locus_tag="VKU83_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI92813.1"
                     /translation="MIRIKAENRWLRRFLLQTPVDLVISDNRYGLYAAGTGCIFITHQ
                     LAIRTGFGRLPDRWLRQANYAFIQRFMACWVPDEAGPDNLAGKLSHPRRMPAVPVRYI
                     GWLSRFGAAAGEPGQPQPAPRIDLLLLLSGPEPQRTILEDLLLAQLHTANLGRVAMVR
                     GLPGGGREPIRAPGVEVHDYLGTAALGTLIGLSRVILSRPGFSTVMDLAALRKRAIFI
                     PTPGQTEQEYLGRYLAGKGWAVCRRQRGFMLPEALAGARKLNPALWPAAGDRQLLSRV
                     IKTVRTQSAADFG"
     gene            1915..>2127
                     /locus_tag="VKU83_04375"
     CDS             1915..>2127
                     /locus_tag="VKU83_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92814.1"
                     /translation="MKACNRDLLVLVKDEHMNEMSMERELELLNELLFHFETMESFCA
                     AHEVFDVNRHKTLHKRDHIQRIIREKE"
BASE COUNT          499 a          602 c          568 g          458 t
ORIGIN      
        1 acggtctccc tgatcaattt tatcaactct caggcttttg acctgtagca gccaatatga
       61 aaaccctttt gttagtccga catgccaaga gcagctggga tagcgcgcat actcccgata
      121 tcgaaagacc gctgagcgac cggggcaaga aagacgcccc tctgatggct aaacgtttgc
      181 ggaaggcagg catccatccg gaccgcctgg tttcaagccc cgcgcgccgg gcccggcaca
      241 ccgcggagct gtttgcccgg gaacttgacg tcgacgaaaa ggagattgac atccggacgc
      301 aactctacca tgcacagccc gccaccttcc aacaagtgat cgcaggcctg aacgatgggg
      361 accaaaccgt cgccctcttc tcccacaatc cggggattac cgactttgtg aacaccctgg
      421 gctccgtcca cctcgacaac atgccgacct gtgcggtatt tgcggtcaaa aaaaggtgcg
      481 agaaatgggc cgaattcctg tccggtaacc cggagttcct tttttttgat tatccgaaat
      541 ccgcggccga ctgagtccta acggtcttga tcacgcggct caatagctgc cggtcgcccg
      601 cagccggcca cagcgccggg ttcaatttcc gggccccggc taacgcctcc ggcagcatga
      661 atccccgttg cctcctgcaa accgcccatc cttttcccgc caggtaccgg cccaggtatt
      721 cctgttcggt ctgcccggga gtcggaataa agatggcccg cttccgtaag gcagccagat
      781 ccatcaccgt gctgaatcca ggccgggaga ggataaccct gcttaagccg atcagcgttc
      841 ccaatgccgc tgtgccgagg taatcgtgca cttcgacgcc gggcgcccgt attggctccc
      901 tgccgccccc aggcaggccg cgcaccatag ccacccggcc caggttcgcc gtgtgcaact
      961 gagcaagcag caaatcctcc aggatcgtcc gttgcggctc gggtccggac agcaacagca
     1021 acagatctat ccggggtgcc ggctgcggct ggccaggttc gccggctgcg gcgccaaatc
     1081 ttgacagcca accgatgtac ctgaccggta cggccggcat ccgccgcgga tgcgacaatt
     1141 tcccggcaag gttgtccggc ccggcttcat ccggtaccca gcaggccata aaacgctgga
     1201 tgaaggcata gtttgcctgc cgaagccagc gatcgggcaa ccggccaaac cccgtcctga
     1261 tcgcgagctg gtgcgtgata aaaatgcatc cggttccggc cgcatacagc ccataccggt
     1321 tatccgagat cacgagatcg accggggttt ggagcaggaa ccgccggagc cagcgatttt
     1381 cggccttgat tcggatcaag atttttggaa tattccaaac cattttcacg accgtaaagg
     1441 cacgattttt ggtgtatttt atgcgatatc cggggatttc gacgaaggaa actgcgggaa
     1501 attcctcctg cagaattgtt ttttgtgcgc cactagccgc gataatgacc ttaaaccctt
     1561 gggtaattaa ttctttaatt attggaatac agcgggttgc atgaccaagc ccccaatcga
     1621 gcggagctat gagaactttt agcattttag catccgggtt gttaaaaata ggcggctgca
     1681 agacgggtca atactttttc gggcctaaaa tagttaaatt gtcaggatta cattcatgaa
     1741 aactacaaaa gcggtattga ttctcttggt attatttagt accattgtaa gcgccggttg
     1801 cagcgcgcag cacgggatcg gtggcagcag caagaagtgc ggctgcgggt tcacaaaagg
     1861 tatggtcggt tattgatcgg caaagcctgg ttagtacgaa aaaatctaat agaaatgaaa
     1921 gcttgtaaca gggatctgct tgttcttgtg aaagatgaac acatgaacga aatgtcgatg
     1981 gagcgggaat tggaattgct gaacgaacta ttgttccatt ttgagactat ggaatcgttc
     2041 tgcgccgccc acgaagtttt cgatgtcaac cgccacaaga ccctgcataa gcgggaccat
     2101 atccagcgca ttatccggga aaaggag
//
LOCUS       DATGKE010000255         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_119339, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000255 DATGKE010000000
VERSION     DATGKE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119339"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VKU83_04380"
     CDS             <1..795
                     /locus_tag="VKU83_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI92815.1"
                     /translation="PRARHLTVTARRSNRNMSLLQTINRLLRPAREPEADPAEAYDIW
                     ARSYDNQPENLILSLDLALCRELLASLPLAGKTIVDVGCGTGRHWPALTKESPTRLVG
                     YDVSKGMLDRLRAKYPGAETRLLSGTALTGLDDSSCDLVFSTLTIAHLADLSAALAEW
                     KRVLKPGGHLIITDYHPTALARGGQRTFRDEGRLIAVRSHIYPIPRLLAVAGRLGLTR
                     VSLVEKKIDASMRPWYERQNALPVFHRFLGVPIIYGLHLTRRDATP"
     gene            782..1957
                     /locus_tag="VKU83_04385"
     CDS             782..1957
                     /locus_tag="VKU83_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI92816.1"
                     /translation="MRLRNVCFPADDTLHDVWLDGGKIVAIRPSHPGPENDTFALPAA
                     VPTLDLGGALAFPGLINSHDHLDFNLFPPLGNRTYRNYTEWGADIHANNRAPIQKILK
                     IPQHLRTQWGLYRNLLNGFTTVVNHGVPLRIDNPLISVFQDCYCLHSVGFEPRWRWKL
                     NHPAKRRRPFALHVGEGTDTAAMREIDELIRWNLFHRPLIGIHAVAMTEKQAGAFHAV
                     VWCPASNHFLLDRTAPVDRLLRRVPVLFGTDSTLTAGWNAWDQLRMARNLRLATEREI
                     LGMLTRAPADAWGLRDRGSLEPGRSADLVIARQRPGATGMNAFFALNPEDFLLVMHEG
                     QIRLFDPSLLGSITEQGLTAEDFEPTGPGGKYVAGDLPGLMAEIRHYCPDVGFPPLP"
     gene            complement(1959..>2859)
                     /locus_tag="VKU83_04390"
     CDS             complement(1959..>2859)
                     /locus_tag="VKU83_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92817.1"
                     /translation="NQWLLGLDPRVIGVWDPLARRDGNGTGGPGGPSIGTAVAGGGVS
                     YRLYMCASAPEESAGSMENGECTEQAISRKALALMNLEYVERVEEHDGSLFLFRVLST
                     QICHIDPMRARLLFEKFYRKPGVTFERLKAVAAAYSYPRRVRIISFHFDDGNNYIFPM
                     DLLADIRPAGRYLLGMRHSNQVLKKIMDVGRIVVAEAPAAYKQVIYKLGRNHSAAPPP
                     LDQLPFPVVATRTWGFVLPEWVESYKEIQIERTLDLGSHMLLCGDWKEEVVLRPATPR
                     LHHLHFLYYLHQKGGRLQYPVMW"
BASE COUNT          554 a          977 c          761 g          567 t
ORIGIN      
        1 cctcgcgcga ggcatctgac cgtcacagcc cgccgttcta ataggaacat gtcgctattg
       61 caaacaatca accggttgct tcggcccgcc cgtgagcccg aagccgatcc agccgaggcc
      121 tacgatatct gggcccggag ctacgacaac cagccggaga acctcatcct gtccctggac
      181 ctggcgcttt gccgggaact cctggcctcc ctgcccctag ccggcaagac tattgtcgac
      241 gttgggtgcg ggacaggccg tcactggcct gcgctcacca aagaatcccc aacccgcctg
      301 gtcggctatg atgtctcgaa aggaatgctc gaccggctcc gcgccaaata ccccggcgcc
      361 gaaacccgcc tcctctccgg tacggccctg accggcctcg acgattcgag ctgcgacctc
      421 gtcttttcca cgcttaccat cgcgcatctc gctgatcttt ctgccgccct cgccgaatgg
      481 aagcgggtgc tgaaacccgg cggtcatctg atcatcaccg actatcatcc cacagccctc
      541 gccaggggtg gccaacgcac cttccgcgac gaaggccggc taatcgctgt ccgcagccat
      601 atctatccca tcccgcgcct cctggccgtc gccggccggc ttggcctgac aagggttagc
      661 cttgtggaaa agaagatcga tgccagcatg cgaccctggt atgaacgcca gaacgcgctg
      721 cctgtcttcc accggtttct tggggtcccg atcatctatg gccttcactt aaccaggcgg
      781 gatgcgactc cgtaacgttt gctttccggc tgacgacaca ctccacgacg tttggctgga
      841 cggcggaaaa atcgtcgcga tccggccttc tcatccgggc ccggagaacg ataccttcgc
      901 ccttcccgcc gctgttccca ccctcgatct cggcggcgcg cttgcgttcc ccggactcat
      961 caactcccac gatcacctcg actttaacct ctttccgccc ctcgggaaca gaacctaccg
     1021 caactacacc gaatggggag cggatatcca cgccaacaat cgtgcaccga tccagaaaat
     1081 cctaaagata ccgcagcacc tgcgcacgca atgggggctg tatcgtaacc ttctcaacgg
     1141 atttacgacc gtcgtcaacc atggcgtccc cctccggatt gataatcccc tcatcagcgt
     1201 gtttcaggat tgctattgcc tccattcggt tggcttcgag ccacgctggc gatggaagct
     1261 taatcacccg gcaaagcgcc ggcgtccctt tgccctccat gtcggcgaag gaacagacac
     1321 ggcagccatg cgcgaaatag atgagctgat ccgctggaac cttttccatc gtccgctgat
     1381 tggtatacac gctgtcgcca tgacggaaaa acaggcgggc gcttttcatg ccgtcgtctg
     1441 gtgccccgcc tcgaaccatt ttctgctgga cagaaccgct cctgtggacc gcctcctgcg
     1501 ccgggttccc gttcttttcg gcactgactc caccctcacc gccggctgga acgcctggga
     1561 ccaactccgc atggcccgca atctgcggct ggcgacggag cgggagatcc ttggcatgct
     1621 gacccgcgcg ccggccgacg cctggggact ccgggaccgg ggaagccttg agcccggtcg
     1681 ctccgcggac cttgtgatcg cccggcagcg ccccggtgcg acgggcatga atgccttttt
     1741 tgcgctgaac cctgaagatt tcctgcttgt catgcacgaa gggcagatca gactgttcga
     1801 cccatccctg ctcgggtcca taacggaaca agggcttact gcagaagatt tcgagccaac
     1861 cggccccggc gggaaatatg tggccggcga tctgccgggc ctaatggcgg agatcaggca
     1921 ttattgcccc gatgtcgggt ttccgcccct gccgtgagtt accacatgac cgggtactgt
     1981 agccgcccac ctttctgatg caggtaataa aggaaatgaa gatgatgcag tctcggagtc
     2041 gccggccgga gcacaacctc ttccttccaa tcgccgcata atagcatatg gctgcccagg
     2101 tcaagcgttc gttcaatctg aatctcctta taactctcca cccattccgg cagaacgaag
     2161 ccccaggtcc tggtcgcgac aaccgggaaa ggcagctgat ccagcggcgg cggggcggcg
     2221 ctgtgattcc ggcccagctt atagatgacc tgtttgtaag ccgccggcgc ttccgctacc
     2281 acgatccggc cgacatccat gatctttttc aacacctggt tggaatgccg catcccgagc
     2341 agatagcgac cggccgggcg gatatcggcc agcaggtcca tcgggaaaat atagttgttg
     2401 ccgtcatcga aatgaaatga aatgatccgc acccttcggg ggtaggagta ggcggccgcc
     2461 accgctttca gccgttcaaa ggttacgccc ggcttccggt agaatttttc aaagagcaat
     2521 cgggcccgca tgggatcgat atggcagatc tgcgtcgaca ggacccggaa caggaacaac
     2581 gacccgtcat gctcctcgac cctctccaca tactccaggt tcatcagcgc aagcgccttc
     2641 cgggatatcg cctgttccgt gcattccccg ttttccatcg accccgccga ttcttccggc
     2701 gccgacgcgc acatgtataa ccgatagctg accccgcctc cggccaccgc cgttccgatc
     2761 gaaggcccgc cgggtccccc ggtcccattc ccatcacggc gggccagggg gtcccaaacc
     2821 ccaatcaccc ttgggtcaag tccgagcagc cattgattg
//
LOCUS       DATGKE010000256         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1158559, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000256 DATGKE010000000
VERSION     DATGKE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158559"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VKU83_04395"
     CDS             <1..370
                     /locus_tag="VKU83_04395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HLI92818.1"
                     /translation="NIKYIYNTLRPETAINRYIDPNWRPHLQTPPFPEYTCGHCTISA
                     AAAETLSSDFGDHFSYTDSTELEFGIPSRSYSSFRDAAIETMRSRFYGGIHYKYCTEL
                     SHTMGEEIGKLVVQRLHMKK"
     gene            complement(405..>2519)
                     /locus_tag="VKU83_04400"
     CDS             complement(405..>2519)
                     /locus_tag="VKU83_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HLI92819.1"
                     /translation="DFTVREQGDEILVVTGSLMISVDRSTAAVVYSDPRGGVILAEDG
                     MRGKTMKPATVVGVPTYTCATQFLSPAGEGLFGLGGHPLDSGSIDYKGRKQDLAIRYM
                     TGAIPVLLSTRGYGLLWDNYSASEFDGTARERYSYTSESGSMVDYYFFYGPEFDHIID
                     SYRRLTGRAPMFAKWAFGLFQSQDRYKTQAEVLAAGAGYRNAHIPVDVIVQDWYYWEP
                     LPIGSHVMNPQGYPDPKGMLDSLHREHLHGMISIWPVFGKGTADYDALAAIHGLTTIT
                     WDNVVTHTFDTYYDVYNPAARKLYWEQARDSLVRRYGWDAWWIDQCEPDNGNLLDARR
                     AAEFYTGHGFDFFNTYSLAHTDGIYQGWRKDIPGKRAFFLARQAFAGEQRNAATLWSS
                     DIQCTWHDFADQIPQGLNACTSGIPYWTSDIGGYHLNWQAPDWSSPDKREMFIRWFQF
                     GSFCPIFRIHGKGERALYSKNWDEATRAVLLNYDKLRYRLLPYIYSLAGAVTQENYTP
                     MRGLAFDFRTDPRVYPIKDQYMFGPAFLVNPVTVPGARTRKVYLPAGEWYDFWTGQQL
                     HGRQTIEAAAPIGRMPVYVRAGSIVPMGPVMEWATERPADTIELRIYPGADGHFALYE
                     DANDGYQYEHGQFATTAMDWVDGRRELLIHGTRGSFPGMLRHRVFQLVLVRKGRGNGV
                     QEETAFDKTVTFSGKELLISLR"
BASE COUNT          543 a          807 c          662 g          507 t
ORIGIN      
        1 gaatatcaag tatatctaca acaccttacg gccggaaacc gctattaacc gttatatcga
       61 tccgaactgg cggccgcatc ttcaaactcc gccattcccc gaatatactt gcggacactg
      121 cacgatctcg gccgcggcag cagagaccct aagcagcgac ttcggcgacc atttcagtta
      181 tacggacagc accgagctcg aatttggcat tccgagccgc agctattcgt cgttccgcga
      241 cgccgcgatc gaaaccatgc gttcccgctt ttacggcggg atacattaca aatattgcac
      301 ggagcttagc catactatgg gcgaggagat cgggaagctt gttgtgcagc gattgcatat
      361 gaaaaaataa tttcagggcc ggcgaccagc cggccctttt cgccctacct tagcgatatc
      421 agcagctcct tccccgaaaa cgtcaccgtc ttgtcgaatg cggtctcctc ctgcacgccg
      481 ttgcctctcc ccttccggac aagcaccagc tgaaagacgc ggtgccggag catccccgga
      541 aaggatcccc tcgtaccgtg gatcagcagc tcccgccggc cgtctaccca atccattgct
      601 gtcgtcgcaa actgaccgtg ctcatattga tacccgtcat tggcatcctc atacagcgca
      661 aaatgaccgt ccgctcccgg atagatcctc aattctatgg tatccgccgg ccgttcggtg
      721 gcccattcca tgacagggcc catgggaacg atcgatcccg cccgcacgta gaccggcatc
      781 ctgccgatcg gcgctgcggc ttcaatggtc tgacggccat gcagctgctg accggtccag
      841 aaatcatacc actccccggc aggcaggtaa accttgcggg tgcgggcccc cggcaccgtc
      901 accgggttca cgaggaaggc tggcccgaac atatattggt ccttgatcgg atacaccctg
      961 gggtccgtac ggaaatcaaa cgccagaccc cgcatcggcg tgtaattctc ctgggtcacc
     1021 gccccggcca gcgagtaaat ataaggcagc agccggtacc tcagcttgtc atagttaagc
     1081 agcacggcgc gcgtcgcttc gtcccagttc tttgaataca acgctctttc gccctttccg
     1141 tgaatgcgga atatggggca aaagcttccg aactggaacc atcggatgaa catctcgcgt
     1201 ttgtccggac tgctccagtc cggggcctgc cagttcaggt gatagccgcc gatatccgac
     1261 gtccagtatg ggatcccgga agtgcaggca ttgagacctt gcgggatctg gtcggcgaag
     1321 tcatgccatg tgcactggat atccgaagac cagagcgtgg ccgcgttccg ctgctctccg
     1381 gcgaaggcct gccgggcgag gaaaaaagcg cgcttccccg gaatgtcctt ccgccagccc
     1441 tggtagatgc catcagtatg cgccagggaa taagtattga agaaatcgaa cccgtgtccc
     1501 gtatagaact ccgccgcccg tcgcgcatca agcaggttgc cattgtccgg ttcgcactga
     1561 tcgatccacc aggcatccca gccgtacctc ctgacaaggc tgtcccgggc ctgctcccag
     1621 tatagcttcc tcgcggcagg gttatacacg tcataatacg tgtcgaaggt atgcgttaca
     1681 acattgtccc aggtgatggt cgtcaatccg tggatcgccg ccagcgcatc gtagtcggcc
     1741 gtccctttac cgaataccgg ccagatcgag atcataccgt ggaggtgctc ccggtgcaga
     1801 ctgtccagca ttcctttggg atcgggataa ccctgtggat tcatcacatg cgagccgatc
     1861 ggcagcggtt cccaataata ccagtcctgc acgatgacat cgaccggaat atgcgcattc
     1921 cggtaaccgg cgccggccgc caacacttcc gcctgtgtct tataacgatc ctgcgactgg
     1981 aacaacccga aggcccattt cgcgaacatt ggcgcccggc ccgtcagcct ccggtaggaa
     2041 tcgatgatat gatcaaattc aggcccgtag aaaaaataat agtccaccat cgacccgctc
     2101 tccgaagtat aactgtacct ctcccgcgcc gtgccgtcga actcagaagc cgaatagttg
     2161 tcccacagta atccataacc gcgggtcgac aagagcacgg ggatggcgcc cgtcatatag
     2221 cggatggcca ggtcctgctt ccggccctta tagtcgatcg aacctgaatc cagcggatga
     2281 cctcccagcc cgaacaagcc ctcgccggcc ggcgaaagga attgcgtcgc gcaggtgtag
     2341 gtcggcacgc ccaccacggt tgccggtttc atggtcttgc cccgcatgcc gtcttccgca
     2401 aggatgacgc cgccccgcgg atcgctgtac acaaccgcgg cggtggaccg gtccacgctg
     2461 atcatcaaac tcccggtaac gacgaggatc tcatcaccct gctcgcggac ggtaaagtc
//
LOCUS       DATGKE010000257         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_407232, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000257 DATGKE010000000
VERSION     DATGKE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407232"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VKU83_04405"
     CDS             <1..824
                     /locus_tag="VKU83_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92820.1"
                     /translation="IQAEREQEKEGQHAAEIERVTQLKNTWIQHQEMVKQTMKALCSR
                     HTIDYVDKVAFKGDPDNTVSICGESIVFDSKSPRGDDLSNFPNYIKEQAEKAKKYANQ
                     ENVKKWIFFVVPVNTLEVIKTFVYHLADYQVFVVTIDSLEPILLSLKKLEEYEFADQL
                     SPEERENICRILGKFAHLSKRRIQIDTFFINQFMELAYKCESDLPADVLEKAIEFERA
                     EKLNPPLEKRAKAIPIADLEKSLTKIQNDAGNKGISLEPASLTDGLNGVPLYSPA"
     gene            895..1365
                     /locus_tag="VKU83_04410"
     CDS             895..1365
                     /locus_tag="VKU83_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92821.1"
                     /translation="MDTDTFHEEQRFTQIWIQIILYFVWAGVTGVSIALVATKKTGPI
                     PAICLFAFMTIFVVVFRNLKLQVSLRKDAISYRFFPIQWKYRNISRVNIESMELIDYN
                     PISDYGGWGIRFGKKGAAYTVKGNHGLYIRRADKSSVLIGTSKPKEMEDFLQKH"
     gene            1598..1960
                     /locus_tag="VKU83_04415"
     CDS             1598..1960
                     /locus_tag="VKU83_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92822.1"
                     /translation="MIFAIILATLDNRFKSMVTDVSHTAAEWIAGTFHYSMYVLLVTI
                     LFVVLFCGPYWLVFQIKNTIVFFLAALLILALDACCYVWLNAAGIWRYGETHFLVGIL
                     TMLICFFKPIRQKILFHF"
     gene            1969..2319
                     /locus_tag="VKU83_04420"
     CDS             1969..2319
                     /locus_tag="VKU83_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92823.1"
                     /translation="MNKIFWGLLLVAIGAVACHRKKFMIRSLPLRQGLSLNWYHYSDI
                     TNFSPDYVEIADRKENRTEVIFEASSICQIQVRSDSILILMVGNTILQRADDNDYHFK
                     IIVDTTCRGAYLYK"
     gene            complement(2386..2892)
                     /locus_tag="VKU83_04425"
     CDS             complement(2386..2892)
                     /locus_tag="VKU83_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI92824.1"
                     /translation="MNINEISIRRELRPGDLGYVMHRHGVLYSREFGYGIEFEAYVGG
                     GLADFYGNYDPKADAVWVCEHGDRIVGFLALMHRAAATAQLRFFYLEPDYRGLGLGKT
                     LMQAYMDFLAGRGYRRSYLWTTHELGAAAALYIRHGFRLTEEKESSAFGKRLREQRYD
                     WIGGDMTQ"
     gene            3156..>3401
                     /locus_tag="VKU83_04430"
     CDS             3156..>3401
                     /locus_tag="VKU83_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92825.1"
                     /translation="MAIRAFLVILMSGALTARAANRFWVAAAASNWNNTANWSNVSGG
                     AGGFSVPGVGDNATFDGGGAGNCTIDLALSLASITVNA"
BASE COUNT          877 a          838 c          825 g          861 t
ORIGIN      
        1 ggatacaggc ggaacgcgaa caggaaaaag agggacagca tgctgcggag atcgagaggg
       61 taacacagct gaaaaacacc tggatccagc accaggaaat ggtcaagcag accatgaagg
      121 cattatgcag ccggcatacg attgactatg tcgacaaggt cgcattcaag ggcgatccgg
      181 acaacacggt atcgatctgt ggcgaatcca ttgtttttga ttcgaagagc ccgagaggcg
      241 acgacctttc caattttccg aactatatca aggaacaggc cgagaaggcg aagaaatacg
      301 ccaatcagga gaatgtcaaa aaatggatct tttttgttgt gccggtcaat acgcttgagg
      361 ttatcaagac ctttgtctat catttggcgg actaccaggt tttcgtcgtt acgattgatt
      421 cgctcgagcc gattctgctt agtctgaaga aactcgaaga gtacgaattc gccgatcagc
      481 tcagtccgga agagcgggaa aacatctgtc gcattctggg gaagtttgct catttgtcca
      541 agcgacgaat ccagatcgac acttttttca ttaatcaatt tatggagctc gcgtacaaat
      601 gcgaaagcga tctgccagca gatgtccttg agaaggccat cgagtttgag cgcgccgaaa
      661 agttgaatcc tccgctggag aagcgggcga aggcaatccc gatcgctgat ctggaaaagt
      721 ccctgaccaa aatccagaat gatgccggca ataaaggaat atcgttggag cccgctagcc
      781 tcacggacgg tctaaatggg gtcccattgt attctcctgc gtaaagctat cacctggata
      841 attttgaaat aactttggag ggccccgccg gcttccctaa caaaaaccag ccaaatggat
      901 accgatacgt ttcatgagga acagcgcttc acgcagatct ggattcaaat tatcctttat
      961 ttcgtgtggg cgggagtgac tggcgtctcc attgccctcg tggccactaa aaagacgggc
     1021 cctatccctg cgatatgcct ttttgcgttt atgaccattt tcgtggtagt cttcagaaac
     1081 cttaaattgc aagtcagcct aaggaaagat gcgatatctt atcgcttctt cccaatacaa
     1141 tggaaatacc ggaatattag tagggtcaat attgaaagca tggagctgat cgactataat
     1201 cccatctcgg attatggcgg ctggggtata cggtttggga aaaagggggc tgcctatacg
     1261 gtaaagggaa atcacgggtt gtatattcga agggccgaca aaagttccgt cctcattgga
     1321 acctccaagc caaaggaaat ggaagacttt cttcagaagc attaagcttt tggaaagctg
     1381 agaattattg agatcgcagt gtggatttta tgtaatgaga agcagcccta tctgaaagat
     1441 aggaacctcc agccaaagaa ttcgaggggc ttgagtatct ttagacgtca tgaacatccg
     1501 aataaccgcc tcggcattcg gaaaaccccc aacgcccaaa cgacctatgc tgcgtataag
     1561 tttcagaaat attgtcctct ttattttttt aaagtatttg atctttgcca tcatactggc
     1621 cacactggat aaccggttca aatcaatggt gaccgatgtt tcccacacgg ccgcggagtg
     1681 gatcgcgggt actttccact attccatgta tgtcctgctc gtcacaatac tgttcgtcgt
     1741 tcttttctgc gggccgtatt ggctggtatt ccaaattaag aatacgatcg tcttttttct
     1801 ggctgccctg ctgatcctcg cgctggacgc ttgttgctat gtctggctga atgccgccgg
     1861 tatctggcgc tatggcgaga ctcatttttt ggtgggcatc ctgacgatgc tgatctgttt
     1921 tttcaaaccg atccggcaaa aaatcctttt tcacttttaa cctgtcttat gaacaaaatt
     1981 ttctggggct tattgctggt ggctatcggt gcggtcgcct gccacaggaa gaaatttatg
     2041 attagatcgt tgccgctgag gcagggactg tcattgaact ggtatcacta tagtgacatc
     2101 actaatttca gccccgatta tgtcgaaata gcggaccgga aggaaaatcg gacagaagtg
     2161 atattcgaag catcatccat ttgtcagatc caagttcgtt cggattccat tctgatcctg
     2221 atggttggaa atacgatctt acaacgagct gatgacaatg actatcattt taaaataatc
     2281 gtggacacca cgtgcagggg cgcctactta tacaaatgaa ctaagtcgca cttcgaataa
     2341 ccatagttct gtagctccgt aacctcctca cccatcttcc ggcagtcatt gggtcatatc
     2401 ccctccaatc cagtcatatc tctgctcacg aagccgcttc ccgaaagccg atgactcctt
     2461 ttcctccgtc aatctgaacc cgtgccggat gtacagggcc gccgcggcgc ccagctcatg
     2521 cgtcgtccag agataagagc gacggtaacc ccggcctgca agaaaatcca tatacgcttg
     2581 catcagagtt ttgcccaacc caagcccccg gtaatccggc tcaagataga agaaccgcaa
     2641 ttgcgccgta gctgcggcgc gatgcatgag cgccagaaat ccgactatcc ggtctccatg
     2701 ttcacaaacc caaacagcat ccgccttcgg atcgtaattc ccgtaaaagt ccgcaagccc
     2761 tcctccgacg tatgcctcaa attcaatccc atagccaaac tcgcgcgaat agaggacacc
     2821 atgccggtgc atcacatagc cgagatcgcc ggggcgcagt tctcgtctta tgctgatttc
     2881 gtttatgttc atgaaagcat tttgctcaaa ggtacaatgg catgaatcct tctttccagg
     2941 gcgatcgccg gtcagaatcc tcctcaggcg caatcccggc cggcgagcag ggacttgatc
     3001 ttcagtgtct tgcgggtgat catcggccta ccatcatgta atatttttac tatacgctag
     3061 tgcttggatt ttacccgggt gccatgccag atacacaatt acacttatga aattacgtta
     3121 ggaatacctg tataatccac tatttcatga aatccatggc gattagagca tttcttgtca
     3181 tcttaatgag cggagcactg actgcgaggg ccgcaaaccg gttttgggtg gccgcggcag
     3241 cttccaactg gaataacacc gccaattggt cgaatgtgag cggcggggca ggcggtttca
     3301 gcgtcccggg cgtaggggac aatgccactt ttgatggtgg cggggcagga aattgcacga
     3361 ttgatcttgc gctttcgctg gcctccatca cggtcaacgc g
//
LOCUS       DATGKE010000258         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_143763, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000258 DATGKE010000000
VERSION     DATGKE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143763"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="VKU83_04435"
     CDS             <1..2782
                     /locus_tag="VKU83_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLI92826.1"
                     /translation="SMKKITISLLTVVAVHSFAFAQPIHNAAIFSAPYKDPTQPIETR
                     VRDLLSRMTPEEKFWQLFMIPGEIPAGQEEHYRNGLFGFQVSAAQKKGDAGAQMLDYG
                     VSDDAAALREKINGLQRFFTDSTRLGIPMIAFDEALHGLVRGGATVFPQAIALSATWD
                     TALVGRVASAIAAEARIRGIRDILSPVINIAADPRWGRVEETYGEDPLLVSAMGVSFV
                     SGFENSSVITTPKHFIANVGDGGRDSYPIFANERLLDEEFFPPFIACIQKGGSRSVMT
                     AYNSLDGTPCSANSWLLTKKLKTDWGFNGFVISDANAVGGANVLHFTSPDIATSGQQA
                     IDAGLDVIFQTAYEHYKLYIQPFLDGRIDTNRINDAVSRVLRAKFELGLFEHPYVTDT
                     DLQSWGNTRSHKALAEQAALESIVLLKNSPGLRTLAGERQPILPLAKTIHSIAVIGSD
                     ATEARLGGYSGPGNGKVSILEGIRERAAQLTHASTNTAIPGAAAKPNIAAGPTPMTDR
                     AIRITYTAGCGRRAQEGSIIPAAYLRLTGEYFNNLTLTGPPTVTRTDPAINFQWTLST
                     PAKGLGHDFYSVRWTGALHAPKTGHYHIGLDGNDGYRLYLDGKLLIDNWEKRSYNTIL
                     QEVSLVQGHDYPIRVEFVEPVGNAHLKLIWDLDAPNQEKQIDSAVAAAAKADVAIVVA
                     GITEGEFQDRASLALPGRQEELIRKVAATGKPVIVLLVGGSAVTMSSWLGDAAAVMAV
                     WYPGEEGGHAVARVLFGDYDPAGRLPISYPLSEAQLPWVYNHLPTGRGDDYNNLSGLP
                     LFPFGYGLSYTHFDYSDLRLDRTEMTRSDSATLTCTVKNSGVREGDEVVQLYLRELVS
                     SLARPVIALKGFQRIHLQPGESQTVSFVVSPALLKALDKDLHPIVEQGTYQLMIGASS
                     RDLRLKTQLQVR"
     gene            2902..>3196
                     /locus_tag="VKU83_04440"
     CDS             2902..>3196
                     /locus_tag="VKU83_04440"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HLI92827.1"
                     /translation="MSLQTLHTLAKTFAPTPKMPILFVGHGNPMNAIEENEFVTGFRT
                     VAAELPQPNAVLCISAHWETRGTFVTAVEHPPTIHDFGGFPRALYQVQYPAPGS"
BASE COUNT          690 a         1075 c          808 g          623 t
ORIGIN      
        1 ttcgatgaaa aagatcacca tttccctgct gacggtcgtc gccgttcatt cctttgcctt
       61 cgcccagcct atccacaatg cagcaatatt ttccgcaccc tataaagatc ctacccaacc
      121 catcgaaacg cgcgtccgcg acctcctctc ccggatgacc ccggaagaaa aattctggca
      181 gctcttcatg atccccggcg agatccccgc cggccaggaa gaacattacc gcaacggcct
      241 cttcggtttt caggtaagcg cagcgcaaaa gaagggcgat gccggcgccc aaatgctgga
      301 ttacggtgtg tccgacgacg cggcagccct gcgcgaaaag atcaatggtc tgcaacgatt
      361 ttttaccgac agcacccgtc tgggcattcc aatgatcgcc ttcgacgaag cattgcatgg
      421 actcgtccgc gggggcgcca ccgtttttcc ccaggccatc gccctcagcg ccacctggga
      481 cacggcattg gtgggccgcg tggcttccgc tatcgccgcc gaagcacgga tccgcggtat
      541 ccgcgatatc ctgtcccccg tgatcaatat cgccgccgat ccacgctggg gccgcgtcga
      601 agaaacctat ggcgaagacc cattgctcgt ttcggccatg ggcgtgtcct tcgtcagcgg
      661 attcgagaac agcagcgtca tcacgacacc taaacatttt atcgccaacg tcggcgacgg
      721 cgggcgcgat agctatccta tcttcgcgaa tgaacgtttg ctcgacgaag aattttttcc
      781 ccccttcatc gcctgcatcc aaaaaggcgg ttcccgttcc gtgatgacag cttacaattc
      841 cctggatggt acaccttgct ccgcgaacag ctggctgctg accaaaaaat taaaaacgga
      901 ctggggcttc aatggtttcg tgatctccga tgccaatgcc gtcggcgggg ccaacgtcct
      961 ccatttcact tcgccggata tcgccacctc tggccagcag gccatcgacg ccggtctcga
     1021 cgtgatcttc cagacggcct acgaacatta caagctctat atccaacctt ttttggacgg
     1081 ccgtatcgat accaaccgga tcaacgacgc cgtgtcccgt gtgctgcggg caaaattcga
     1141 gctcgggctc ttcgaacatc cctatgtgac agacacggat ctccaaagtt ggggcaatac
     1201 cagatcacac aaagcgctgg ccgaacaggc cgcgctggaa tccatcgtat tgttaaagaa
     1261 tagtcccggc ctccggaccc tcgcagggga aagacagccc atcctgccgt tggcaaaaac
     1321 aatccattct atcgccgtga tcggctccga tgctaccgaa gcccgcctgg gtggctatag
     1381 cggtcccggc aacggtaagg tcagcatcct cgaaggtatc cgcgaacggg cagcgcagct
     1441 cacgcacgcg agtaccaaca cggcgatccc cggcgccgcg gcaaaaccca acatcgcggc
     1501 aggcccaacc ccgatgacag accgcgccat ccgcatcacc tataccgccg gctgcggcag
     1561 aagagcgcaa gaggggagca tcatccccgc ggcatatctc aggctcaccg gagaatattt
     1621 caataacctc accctcaccg gaccgcccac cgtgacgcgg accgatccgg ccattaattt
     1681 tcagtggacc ctttcgacgc ccgcaaaagg tctcggtcac gatttttatt cggtgcgatg
     1741 gaccggcgcc cttcacgcac caaagaccgg ccattaccac atcggcctcg atgggaacga
     1801 cggctaccgg ctttatctcg acggcaagct gctcatcgac aattgggaaa aacgcagcta
     1861 taacacgatc ctccaagaag tctccctggt acaaggtcac gattatccga tccgcgtcga
     1921 atttgtcgaa cctgtcggca atgcccacct gaagttgatc tgggacctcg acgcgcccaa
     1981 ccaggaaaaa cagatcgatt ccgccgtcgc ggccgccgca aaagcggacg tcgctatcgt
     2041 cgtcgccggc atcaccgaag gcgaattcca ggaccgcgcc tcattggcgt tgcccggccg
     2101 gcaggaagag ctcattcgca aggtggccgc caccggcaaa cccgtgatcg tgctgctggt
     2161 gggcggtagc gctgtcacca tgtcttcctg gctcggtgac gccgccgcgg ttatggcggt
     2221 atggtatccc ggcgaggaag gcggtcatgc cgtggccagg gttttgttcg gtgactatga
     2281 tcccgccgga cggctgccca tcagctatcc cctctccgaa gcccagctgc cttgggttta
     2341 caaccacctg ccgacaggcc gcggcgacga ttacaacaat ctcagcgggc tcccgctctt
     2401 ccctttcggt tacggtctta gctataccca tttcgactat agcgatctgc gcctcgacag
     2461 aacagaaatg acccgcagcg attcggccac cctcacctgt accgtgaaga actccggcgt
     2521 ccgggagggt gacgaagtag ttcagctcta cctccgcgag cttgtctctt ccctggccag
     2581 acccgtcatc gccctgaaag ggtttcagcg tatccatctg cagccgggcg aatcccaaac
     2641 cgtttccttt gtcgtttcac ccgcgttgct caaagccctc gacaaagatc ttcaccccat
     2701 tgtcgagcag ggaacctacc agctgatgat cggcgcctct tcccgcgacc tccggctgaa
     2761 gacgcagctg caagtgcgat aaccggcaac ccccgaaatc cggcatgtcc actgatagga
     2821 acgcccgccg cgtcgcgcat ccgctgacac gcaacccccg aaatccggcc acatccgcta
     2881 attcgatacc tttgtatcgc catgagcctt caaaccctcc atacactggc aaagaccttc
     2941 gcgcccacgc ccaaaatgcc catccttttc gtaggacacg gcaatcccat gaacgccatt
     3001 gaagagaacg aattcgtcac cggtttccga accgtcgcgg cagaattgcc ccaacccaat
     3061 gccgtgctgt gcatttccgc gcattgggag accaggggca ccttcgtgac ggccgtcgag
     3121 catcccccca ccatccacga cttcggcggt ttcccacgcg cgttatatca agtacaatat
     3181 ccggcccccg gcagcc
//
LOCUS       DATGKE010000259         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1343496, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000259 DATGKE010000000
VERSION     DATGKE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343496"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VKU83_04445"
     CDS             complement(<1..482)
                     /locus_tag="VKU83_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92828.1"
                     /translation="MVLSTVSFRKLFCFVLLPVLSSLAACNSNYTPKRRGYFRIDFPA
                     HQYQQFDRPGYPYSFEYPVYANVVKDTSFFDTVPENPYWINVDFPRFNARIYVSYKKI
                     GPNKYDKLTDDAFKMTYKNTYKASSIDQTVIHTPNGIGGIFFHVGGNAATAQQFFLTD
                     T"
     gene            complement(490..1800)
                     /gene="gldE"
                     /locus_tag="VKU83_04450"
     CDS             complement(490..1800)
                     /gene="gldE"
                     /locus_tag="VKU83_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HLI92829.1"
                     /translation="MSIFSFRLLVINPQATTILAIIILFLLILSFVISGSEVAFFSLT
                     FKDINILKTKQDNSWKRIVSLLEEPKTLLASMLICNSIINIAIIILGNFLIDEMVLLK
                     QDFLVFEFLIKALLIGFVILLFGQVMPKIWATQNNLQFAYYTSGVIELIHLLFRRVGN
                     WAVLRSEGLERAFGGKGSALSPQQLGRQIDQVTKNEASDEERNILKGLLEFGGITVKQ
                     VMRTRLEVCGVRYELDFAGLKRRVDELHYSRLPVYKNSLDELAGMIHTRDLIALLGQP
                     DDFDWHSLLRPIYFVHEQKLISSLMQEFQQKRIHFAVVVDEFGGTSGIVTLEDIVEEV
                     IGDIRDEFDEEERHSRRLEDGTWIFEGRTMLNDVCKIMNIPVDTFDKVKGESDSLAGL
                     ILELSGNIPKPNDIVVSGDFEFTILDADSSRIKKVKVTVGPAKS"
     gene            complement(1873..2316)
                     /gene="ssb"
                     /locus_tag="VKU83_04455"
     CDS             complement(1873..2316)
                     /gene="ssb"
                     /locus_tag="VKU83_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLI92830.1"
                     /translation="MRGVNRVMLIGNLGKDPDVQFLDGNIAVAKFSLATTETFKDRAG
                     KLISQTEWHTVVLWRGLAELAQKYLHKGSLVYIEGRLRTRSWEDKEGNKKFATEVVGD
                     NLIMLDKRTDGTSSHPPLHHHEGMEGLGNSETPPALGEPSEDLPF"
BASE COUNT          585 a          608 c          650 g          611 t
ORIGIN      
        1 gtgtcggtaa ggaagaactg ttgtgcggtt gccgcgttac cccctacgtg aaaaaagatc
       61 ccaccgatgc cattcggcgt atggatcacg gtttgatcga tggaactggc cttgtaggtg
      121 ttcttatagg tcattttgaa ggcatcatcg gtcagcttgt cgtatttgtt cgggccaatc
      181 ttcttatagc tgacatagat ccgggcattg aagcgcggga agtcgacgtt gatccagtac
      241 ggattctcag gcaccgtatc gaagaaactg gtgtctttga cgacgttcgc atagaccggg
      301 tattcaaaac tgtaagggta tcccggacga tcaaactgct gatactgatg tgcggggaaa
      361 tcgatcctga aatacccgcg gcgtttcggc gtatagttgc tgttgcaggc tgcgagggac
      421 gacaagaccg gaaggagaac gaagcagaac aacttgcgaa aggatactgt tgataacacc
      481 atattgggtt taagatttgg ccggaccgac ggtaaccttg acctttttga tccggctgct
      541 gtccgcgtcc aggatagtaa attcgaagtc gccggagacg acaatgtcgt tgggtttcgg
      601 gatattacct gaaagttcca ggataaggcc cgcgagcgaa tcgctttccc cttttacctt
      661 atcgaacgta tcgaccggga tattcattat tttgcagaca tcgttcagca tggtgcggcc
      721 ttcaaaaatc caggtcccgt cttccagccg cctgctatgg cgttcttctt catcgaattc
      781 atcccggata tcgccgataa cttcctcaac aatatcctcc agcgtaacga taccgctggt
      841 gccgccaaat tcgtccacga cgacggcaaa atgaatgcgc ttttgctgga attcctgcat
      901 gagactggaa atcaacttct gctcatggac gaagtaaatg ggacgcagca gggagtgcca
      961 gtcgaaatca tccggctggc cgagcagggc gatcaggtcg cgggtatgga tcatgccggc
     1021 aagctcgtcg aggctgttct tatacaccgg cagccgggag tagtgcaatt cgtcgaccct
     1081 ccgtttcaat cccgcaaaat cgagttcata ccttacgccg caaacctcca ggcgcgtccg
     1141 catgacctgt ttcacggtga taccgccgaa ttcgagcagc cccttcagga tgttccgttc
     1201 ctcgtccgac gcctcgttct tcgtgacctg atcgatctgg cggccaagct gctgcgggct
     1261 cagtgcggac cctttgccgc caaaagcgcg ttcgaggccc tccgaccgga gaaccgccca
     1321 attgcccacc cgccggaaca gtagatggat cagttcgatg acgccggatg tataataagc
     1381 aaattgcagg ttattttggg ttgcccagat cttgggcatc acctggccga aaagcaggat
     1441 gacaaaaccg atgagcagcg ccttgatcag aaattcgaat acgaggaaat cctgcttgag
     1501 aaggaccatc tcgtcgataa gaaaattgcc caggatgatg atggcgatat tgatgatgct
     1561 gttgcaaatc agcatggacg ccagcaaggt cttcggttct tcgaggagcg aaacgatccg
     1621 cttccaggag ttgtcctgtt tggttttaag gatattgatg tccttgaatg tcagagaaaa
     1681 aaacgccact tcggagccgg atatcacaaa ggaaagaatg agcaggaaaa ggattatgat
     1741 ggccaggatg gtcgtcgcct gggggttgat tacgagcagc ctgaaagaaa atatggacat
     1801 ccggccaaat ttaatgaatt ctgtcatatc taataggctg cccgccagag ggcctcttgt
     1861 ctcccgcggc ggttaaaaag gaaggtcttc ggagggttcg ccgagcgcag gcggcgtttc
     1921 agaattgccg aggccttcca tcccttcgtg atggtgaagc ggagggtgcg aagaagtccc
     1981 atcggtgcgt ttgtccaaca tgatcaggtt gtctccgacg acttccgtgg caaatttctt
     2041 gttgccttcc ttgtcttccc aactgcgggt ccggagccgt ccttcgatgt atacaaggct
     2101 gcccttgtga agatattttt gggctagttc agccagaccg cgccacagga cgacggtatg
     2161 ccattcggtt tgagagatca gctttccggc cctgtcttta aacgtctcag tcgttgccaa
     2221 tgagaacttt gcgactgcga tattaccgtc gagaaattgt acgtccgggt cctttcctaa
     2281 attgccaatc aacatcaccc tgtttacgcc tctcatacac gaagtttgat actaaaatta
     2341 cccctttttt tcaggaatag ataaattttt gttgctctgt tgcgaggagc cacttgttaa
     2401 tgaacttcgg aaatgcatag cccgacaggc tgctttttgg actgaggaaa taat
//
LOCUS       DATGKE010000260         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1263185, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000260 DATGKE010000000
VERSION     DATGKE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263185"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VKU83_04460"
     CDS             complement(<1..478)
                     /locus_tag="VKU83_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HLI92831.1"
                     /translation="MKNSNAILETYRHGQSIWLDYIDRGLIRSGELEHMITDQGLTGM
                     TSNPTIFEKSISGSDQYNEQIKELIARGKGNEEIFYGLALDDVGAAADLFRRVFDRHP
                     GPGGDGFVSIEVSPRLARDTAGTIQQGELLWSLARRQNVMIKVPATAEGIPAIRRLT"
     gene            606..1838
                     /locus_tag="VKU83_04465"
     CDS             606..1838
                     /locus_tag="VKU83_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HLI92832.1"
                     /translation="MATTTASSPPRTAAANRPVQRYRIESIDLVRGAVMIIMAIDHCR
                     DHLLKGNPHPLNMATTTPLLFFTRWITHFCAPVFVFLSGVSVSLAAARRTRRQLSSLL
                     ITRGLWLVLVEVLLISAGASLDPALHIIILQVIWVIGVSMLLLGLLIGLRASRVAIGL
                     IGAIIFLGHNVFDHADMLNRDFIWRLLVSAKGFDFKTVLRLPGEHLVVVIYALLPWTA
                     VMFLGYAAGPLYSQGFDAARRRRLLYGAGLSLTAAFIILRAFNLYGDPAPWSVQKNAV
                     YTFMSFLNVTKYPCSLLYLLMTLGPALVALAMAERVRNRFTTAIVAYGNVPFFFYVIH
                     WYVIGAITITLFYLQGFHSAQAAAPGAIFHFDPPEFGLSLAGVYTVWLIVIVVMYRPV
                     RWFSSYKRRNRRWWLSYV"
     gene            complement(1872..>2999)
                     /locus_tag="VKU83_04470"
     CDS             complement(1872..>2999)
                     /locus_tag="VKU83_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92833.1"
                     /translation="INPRKVLVVLQFTFAIILITSTMIVEHQLQYARDRDAGYNRNRL
                     AFIFAQGDILPHFDAFKHDLLTSGAAVGVTRTFSPMTLVWGETTGYSWPRSKPADKRV
                     YFTQFESDADFVKTTGARLLAGRDLDIRNYPADSFGVLLNETAVRAMRLDNPVGALIT
                     DGSGAHLHVVGVVQDFIIADPYAPVVPMIIRGLTTGYPVIHFRLNPNRPLAADLSAAE
                     EIFKRYNPQYPFEINFVDDVYNAKFKTEQQEGTLGSLFAILTIFISCLGLFGLAAYMA
                     ESRTREIGIRKVLGASVAGITALISGDFIKLVLIALVIATPVSWLVMNHWLRGFAYRI
                     HISGWTFLLAGALSTGIALLTVGYQSVRAGLTSPVKSLRTE"
BASE COUNT          588 a          824 c          889 g          698 t
ORIGIN      
        1 ttgtcagtcg ccggatggcg gggatgcctt ccgcggtggc cggcaccttg atcataacgt
       61 tctgccggcg ggccaggctc cacaggagtt ccccttgctg gatggttccg gcggtatctc
      121 ttgccagcct gggcgagacc tcaatactta caaagccatc gccgccgggc ccgggatgcc
      181 ggtcgaacac tctcctgaaa agatcggcgg cagcccccac atcgtccagg gcgaggccgt
      241 agaagatctc ctcgttacct tttcccctgg ctatcagctc ttttatctgc tcattatatt
      301 gatcactgcc acttatcgac ttctcgaaaa tagtgggatt cgatgtcatg ccggtaaggc
      361 cctgatccgt gatcatgtgt tccagctcgc ctgaccggat aagaccgcgg tcgatataat
      421 cgagccagat gctttgccca tgccggtatg tttcgaggat cgcattggaa tttttcatgg
      481 ctgccggggc ggttttagct ggtccccgga aatggccgca atccctgtgc cgttgaataa
      541 aaagggagct tagaatccca tattttaggg atgggcggtc cggatatttt aagtatcttg
      601 gtgaaatggc caccacgact gcttccagcc ctccccggac agccgccgcg aaccgtccgg
      661 tccagcgcta ccgcatcgag tctatcgatt tggttcgcgg cgcagtgatg atcatcatgg
      721 ccatcgacca ctgccgcgac catctgctga agggcaatcc tcacccgctg aacatggcga
      781 caaccacccc cctgctcttc tttaccaggt ggatcaccca tttctgcgcg ccggtcttcg
      841 tctttctgag cggggtgtcc gtgtcactgg cggcggcaag gcgaacgcgg cgacagttgt
      901 catccctatt gatcacccgg gggctctggc tggtcctggt cgaggtcctg ctcatttccg
      961 ccggagcttc gttggatccc gcgctccaca tcatcatttt gcaagtcatc tgggtgattg
     1021 gcgtaagtat gctgttgttg ggactgctga tcggacttcg ggcctcccgc gtcgcgattg
     1081 gtctgattgg ggcaataatc tttctgggac ataacgtttt cgaccatgcg gacatgttga
     1141 accgtgactt tatctggcgt ttattggtgt cggcgaaagg tttcgatttc aagaccgtct
     1201 tgcggcttcc aggcgaacat cttgtggtgg tgatttacgc cttactgccc tggactgcgg
     1261 tgatgttcct gggctatgcc gccggacccc tttacagcca gggttttgat gccgccaggc
     1321 gacggcggct gctctatggc gccggcttgt ccctgaccgc tgcatttatt atcctgcggg
     1381 cattcaatct gtacggcgat ccggcgccat ggtcggtaca gaagaacgca gtgtatacct
     1441 tcatgtcctt cctgaatgtg acaaaatacc cctgttccct gctgtacctg ctgatgacgt
     1501 taggtccggc gctcgttgcg ctcgcaatgg cggagcgggt acgaaatcga tttaccacgg
     1561 cgatagtggc ttacgggaat gtgcccttct tcttttatgt gatccattgg tacgtcatcg
     1621 gagcgatcac gatcacgctt ttttacttgc aggggttcca ttccgcgcag gctgccgccc
     1681 ctggggccat ctttcatttc gatccgccgg aattcggatt atcgcttgca ggcgtttaca
     1741 cggtctggct gatcgtaatc gttgtgatgt atcgcccggt caggtggttc agcagctata
     1801 aacggcggaa tcgcaggtgg tggttaagct atgtttagcg ccgggcccct tcggggcccg
     1861 catccccgca actattcggt acggagcgat tttaccggac tggttagtcc ggcacggacg
     1921 gactggtagc cgacagttag gagcgcgata ccggtcgaga gcgcgcccgc cagcaggaag
     1981 gtccagccac tgatatggat ccgataggcg aacccgcgga gccagtgatt catcaccagc
     2041 caggataccg gcgtggcgat gacgagggcg atcagcacca gcttgatgaa atcaccggaa
     2101 atcagcgcag tgatgccggc gactgaggcg ccaagcactt tgcggatgcc gatctcccgg
     2161 gtccggcttt ccgccatata ggcggcgaga ccaaagaggc caaggcagga aatgaaaatg
     2221 gtgagaatgg caaagaggct gccaagcgta ccttcctgct gctccgtttt gaatttcgca
     2281 ttatagacat catcaacgaa gttgatttcg aacggatact gtgggttgta tcgcttgaag
     2341 atctcttcgg cggcgctcag atcggcggca agtggacggt tgggattaag ccggaaatgg
     2401 atgaccgggt agccggtcgt cagtccgcgg atgatcatcg gcaccaccgg tgcataggga
     2461 tccgcgataa taaaatcctg gacgacgccg accacatgaa ggtgggcgcc ggagccatcg
     2521 gtgataaggg cgccgacagg attatcgagt cgcatggcgc gaacggcggt ttcattgagc
     2581 aggactccga agctgtcggc cgggtagttg cggatatcca ggtcacggcc ggccaggagc
     2641 cgcgcaccgg ttgttttgac aaagtctgcg tccgattcaa attgggtaaa atagacgcgc
     2701 ttatcggccg gcttgctccg cggccaggaa tatcccgtcg tctcgcccca gaccagcgtc
     2761 atgggggaaa acgtcctcgt gacccccacg gcggcgccgc tggtaagcaa atcatgtttg
     2821 aacgcgtcga aatggggaag gatatcgccc tgggcgaaaa tgaaagccag gcggttccgg
     2881 ttgtagccgg cgtcgcggtc gcgggcatat tgcaactggt gctcgacaat catggtggac
     2941 gtgatcagga tgatagcgaa agtaaattgc aggacaacaa gcactttgcg cggattgat
//
LOCUS       DATGKE010000261         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1227115, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000261 DATGKE010000000
VERSION     DATGKE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227115"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VKU83_04475"
     CDS             <1..563
                     /locus_tag="VKU83_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HLI92834.1"
                     /translation="GGICSPHAVPGFWHNFSPWIKKASYMKRTGISCLLFLLACQSPS
                     KHSPAPQVDSVAPVPARPITKDTTTIGGTWYLEPVPPSDSTGNPPWIHLGLELSRFNG
                     YTGCNSMHGKFYYSAVDSSISFGERIIVGRKFCQGYNEAAFLKGLKSTGRYRLLNGVL
                     ILMMDNHTELSRWRRGKPAGRKTTVT"
     gene            626..898
                     /locus_tag="VKU83_04480"
     CDS             626..898
                     /locus_tag="VKU83_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92835.1"
                     /translation="MTTFINLTRELLKSEVFSHLPDEEIARLHWMILRAQQDEELPLQ
                     PLLTFWYRGNYYSSNASPGLLQSCNEYLQRMGQPLIDVYGEEFYEA"
     gene            complement(886..1263)
                     /locus_tag="VKU83_04485"
     CDS             complement(886..1263)
                     /locus_tag="VKU83_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92836.1"
                     /translation="MAKELYFDISSEKTGGSLYRVREANGDASFLYNYSVTDARNGEV
                     EVFDTWYVSWEAFWKALTKNAEWYYLHPLFVHPEQREFLREQLKAVDWSIHADKKWQE
                     SHRRQWKKVLEDPGTYYQGPQAS"
     gene            1320..1478
                     /locus_tag="VKU83_04490"
     CDS             1320..1478
                     /locus_tag="VKU83_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92837.1"
                     /translation="MSLIPNNKKKKDGKPLAGPKSNIPGFPGGKGKANTKAVNKNTRQ
                     TGGSQRGS"
     gene            1509..>3088
                     /locus_tag="VKU83_04495"
     CDS             1509..>3088
                     /locus_tag="VKU83_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLI92838.1"
                     /translation="MLDYLSELNDRQREAVLHRDGPIMIVAGAGSGKTKVLTTRIAHL
                     LSQGVSAFNILALTFTNKAAREMKERVEKILGNNEARNLYIGTFHSVFARILRGEAHH
                     LGYDRNFTIYDTDDAKSVLKTVINELNLDDKHYKPNTVYNRISAAKNALVGPLEYAKD
                     YAIQQEDMRSNRPAIAQIYDAYTKRCFKNGAMDFDDLLMKMYELLKNFPESLHKFQHK
                     FKYILIDEYQDTNPAQYEIIKLLGAAHENVCVVGDDAQSIYSFRGATIENILQFQKDY
                     DDVRIVKLEQNYRSTQSILNVANEVIRNNKGQIPKNLWTENATGEKIRLVRLMTDNDE
                     GKYVADTIQEQRLRNHYNNRDFAILYRTNAQSRAFEESLRRMGIPYTMYGGISFYQRK
                     EIKDFLGYLRVIVNPRDEEALKRIINYPARGIGKTSIDRAVLAANERGLYLWEVLQRA
                     AEFGFKAGTLEAVENFVLMIRSFSSMLASKNAYEVAQHVGKQTNLVRELFNDKSTEGV
                     ARYENIQELLNSIKEFTD"
BASE COUNT          797 a          860 c          777 g          654 t
ORIGIN      
        1 tcgggggaat ttgttcccca cacgccgtgc cagggttttg gcataatttt tccccctgga
       61 tcaaaaaggc ctcctatatg aaaagaaccg ggatctcatg cctgctgttc ctccttgcct
      121 gtcaaagccc ctcgaagcac agcccggccc cgcaggttga ttcggtagcc ccggtgcccg
      181 cgcggcccat tacgaaggat acgacgacga tcgggggcac ctggtatctc gaacccgttc
      241 ccccatccga ttcgaccggg aacccgccgt ggatccatct cggcctcgag ctctcccggt
      301 tcaacggata taccggttgt aactcgatgc atggaaagtt ctattacagc gccgtagaca
      361 gcagcatttc tttcggcgaa cggatcattg tcggcaggaa gttctgccag ggatataatg
      421 aagccgcctt tctaaaaggc ctgaagagta ccggccggta ccgtctgctg aacggtgttc
      481 taattttgat gatggacaat cataccgagc tgtcccgatg gcgccgggga aaaccggcag
      541 gccgaaaaac tacggtaacg taacagctta ttcatattcc ggtaatatta ttgcatgaac
      601 tttccgttaa atcaatatta tgtccatgac taccttcatt aatctgacga gggaattgct
      661 caaaagcgag gtcttcagcc atctgccgga tgaggagatc gcccggcttc actggatgat
      721 actgcgggca cagcaggacg aggagctgcc gctgcagccg ctgctgacat tctggtatcg
      781 cgggaactac tattcgtcga acgcgtcgcc cgggttactc cagtcctgca acgaatacct
      841 gcagcgaatg ggtcagcccc tcatcgacgt atacggggaa gaattttatg aggcctgagg
      901 gccctggtaa taggttcccg gatcttccag taccttcttc cactgccgcc gatggctttc
      961 ctgccacttc ttatcggcgt gaatactcca gtccaccgct ttcagctgtt ctctcaaaaa
     1021 ttcacgttgc tccggatgca cgaacagcgg atggagataa taccattccg cattcttcgt
     1081 cagggccttc cagaatgcct cccagctcac ataccaggta tcgaagacct cgacctcgcc
     1141 gttccgggca tcggtaacgc tgtaattata cagaaaagat gcgtcgccat tcgcctcccg
     1201 cacccggtag aggctgcccc cggttttctc cgaactgata tcgaaataga gttccttcgc
     1261 catgaaaaaa aacactaatt tcgggattaa atttctgtta tcttagaaaa gtaaccgata
     1321 tgtcactcat tccaaacaat aagaagaaga aggacggcaa gcccctcgcc ggcccgaaga
     1381 gcaatatccc gggatttcct ggcggcaagg gaaaggccaa tacgaaagcg gtgaataaga
     1441 atacccgcca aaccggtggc tctcaacgcg gttcctgaaa ttgaggcgga tttgactacc
     1501 ttcgtaaaat gcttgattat ctaagtgagc tgaatgaccg gcagcgggaa gcagtgttgc
     1561 atagggatgg gccgatcatg atcgtcgctg gcgccggcag cggaaaaacc aaggtgctga
     1621 cgacccgcat tgcccatctg ctcagccagg gggtgagcgc gttcaacatc cttgcgctga
     1681 cctttaccaa caaggcggcc agggaaatga aggaacgcgt tgaaaaaatt cttggaaata
     1741 acgaggcgcg caacctatac atagggactt ttcacagtgt tttcgcccgg atcctccgcg
     1801 gtgaagccca tcaccttggt tatgaccgca atttcacgat ctatgacacg gacgatgcaa
     1861 agagcgtttt gaagaccgtc atcaacgaac tcaatctcga cgacaagcat tacaaaccca
     1921 atacggtata taaccgcatt tccgctgcca agaacgcgct cgtcggcccc cttgaatatg
     1981 ccaaagacta tgccatccag caggaagaca tgcgcagcaa ccggcccgcg atcgcccaga
     2041 tctacgatgc ctatacgaaa cgctgtttta agaacggcgc catggacttc gacgacctgc
     2101 tgatgaaaat gtatgaactt ctgaaaaact tccccgaaag cctccacaaa ttccagcaca
     2161 agttcaaata catcctgatc gacgaatatc aggataccaa cccggcacag tacgagatca
     2221 tcaagctgct cggcgctgcg catgagaacg tctgcgttgt gggcgatgat gcccaaagta
     2281 tctacagttt tcgcggcgcc acgatagaaa atatcctgca atttcaaaaa gactatgacg
     2341 acgtgaggat cgtgaagctg gagcagaact accggagcac ccagagcatc ctgaacgtcg
     2401 ccaacgaagt catccgcaac aacaagggcc agatacccaa aaacctttgg accgaaaacg
     2461 ccaccggtga aaagatccgc ctcgtccggc ttatgaccga caacgacgag ggtaaatatg
     2521 tggcggatac cattcaggag cagcgcctgc gcaaccacta taataaccgg gatttcgcca
     2581 tcctctaccg gaccaacgcc caaagccgcg cttttgaaga aagcctccgc cggatgggta
     2641 tcccttatac catgtacggg ggaatcagct tttaccagcg gaaagagata aaggacttcc
     2701 ttggctatct ccgcgtcatc gtgaatcccc gggacgagga agccttgaag cggatcatca
     2761 attatccggc gcggggcatc ggtaaaacct ccatcgaccg ggcggtcctc gccgccaatg
     2821 agcgcggact ctatttgtgg gaggtgttgc agcgggcggc tgaattcggt ttcaaggccg
     2881 gcaccctgga agccgtcgag aattttgtcc tgatgatcag gagcttctcg agcatgctgg
     2941 catcgaagaa cgcttacgag gtcgcccagc atgtcggcaa acagaccaac cttgtcaggg
     3001 aactgtttaa cgataaatcc acggaggggg tcgcccggta cgagaatatc caggagctgc
     3061 tgaactccat caaggaattc accgatat
//
LOCUS       DATGKE010000262         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_755020, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000262 DATGKE010000000
VERSION     DATGKE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755020"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VKU83_04500"
     CDS             complement(<1..905)
                     /locus_tag="VKU83_04500"
                     /inference="COORDINATES: protein motif:HMM:NF023866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HLI92839.1"
                     /translation="MRSVLLVLSFLLICSSSPAQYYLTGEVLDIHGDKLQNVSISSHS
                     TGSVYQTDRIGEFRIYSRTMDDTLTFAVDGYEPYTTQVHTTGYLLVTLRMNSFSADDD
                     QRRPVTVIRVPVWDSASMGMRVGAGGELFGPGSLGGASSTALSGVTLRAENPFVPQWA
                     TTSFTGAVRRESYSTIRRFLDMGLEVPADAVKVEEMLNYFNFSYDAPGARDLFTASST
                     VLTCPWNGGHRLLALRVCARKLDIRNRPPANLVLLIDASGSMDMPNKLPLVKAGVRLL
                     VSNLREIDTISLVEFGREVRVLFAG"
     gene            1053..>3004
                     /locus_tag="VKU83_04505"
     CDS             1053..>3004
                     /locus_tag="VKU83_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HLI92840.1"
                     /translation="MLKRYPGGNSILFIVALLLPWTVFAQPVVSAVSPAAGPVNSTVT
                     ITGSNFSLTPSANAVWFGSVRVPVTAASATSLTLTVPTGTSYEPIIVTTGGLTSAPFA
                     PFITTFSDTGQFTPAAFSTRTDVPTGSGPQWICNADLDGDGKPDIIVANGDSNTVTVY
                     HNNSTPGTVSFAEAGSYMMGTSGYPIGVVAGDLDGDGKPDLVISNYYTQTLTVYLNAS
                     TPGNIVMDSVLSVPSANYILGAAIADLNGDGKPEVIVASQGSNMLAVYTNSSIIGKIS
                     FSNETPIMSPSGGSPFKVVVGDLDGDGKPDLAAANSYLGTVSVYLNTSPAEGPISFAA
                     NVDFATGNFPEGMAIGDLDGDGKPDMVVANNTDNTLSLLRNTSTPGNLSFASQLTVNS
                     GYAAYDIALGDFDGDGKPDIAVVDQYNNTVSIHRNVSTPGAIAVSPNVDYNTGNIPYA
                     ISSADFDGDGKPDLATCNDADNTFTVLRNKGSNESAITSFSPATGTQGTVVTVVGVNL
                     TGATSVSFGDSAASSFTVVSPDTITAVVGTGATGAVTVVTAGGSASLAGFVYGTQPVI
                     SGFSPDSAAQGASVTITGKGFTGTNSVLFGGTPAVSFTVSNDSTIMATVGAGTTGAIT
                     VSGPQGSATAGGFVYLVVAPPPIVLLS"
BASE COUNT          648 a          946 c          776 g          634 t
ORIGIN      
        1 atgcccgcaa acagaacgcg cacttcccgg ccaaactcta ccaatgaaat cgtatcgatc
       61 tccctcaggt tactcaccag caggcggacg cctgccttca ccaatggtag tttgtttggc
      121 atatccatcg atcccgaagc atcgatgagc aagaccaggt tggcgggcgg ccggttccgg
      181 atatccagtt tgcgcgcgca aaccctcaac gccagcaggc ggtgacctcc gttccagggg
      241 caggtaagca cggtggagct cgctgtgaag aggtctctgg cgccgggcgc gtcatacgaa
      301 aaattgaagt agttcaacat ctcctcaacc ttcaccgcgt ccgccggcac ttccagtccc
      361 atatccagga agcgtcggat ggtgctgtat gactcccgcc gtacggcgcc ggtaaaggag
      421 gtcgtcgccc attgcggcac aaacggattt tccgcccgca gggtgacccc tgacaatgcc
      481 gtcgacgagg cgccgcccag actaccggga ccgaacagtt ctccgcctgc tccgacccgc
      541 atgcccatcg aggcgctgtc ccagaccggc accctgatca cggtaaccgg ccgtcgctgg
      601 tcatcatccg cagaaaacga attcattctc agtgttacca gcagataccc tgtcgtatgc
      661 acctgcgtcg tatatggctc ataaccatct accgcaaagg tcagcgtatc atccatcgtg
      721 cgggagtaaa tgcggaattc gccaatgcga tccgtctggt acacggagcc ggtcgaatgc
      781 gaagaaatgg agacgttttg taatttatcg ccgtgaatgt caaggacctc accggtcaga
      841 taatattggg ccggcgaaga cgaacagatc aataggaagg acaagacgag taaaactgat
      901 ctcaaatagg tggtttattg tatatccgca aagttaatgc ccgcttgtca agaaaaaaat
      961 gccttaatcc gccgtcatgt ataataaata ggcgatatac gtagttcttg ttccggattg
     1021 tttctattat cttggctcaa attcaatacc ccatgctgaa acgctaccct ggtgggaaca
     1081 gcattctctt tatcgttgcc ctgctcttac cgtggactgt atttgcccag cccgtcgtat
     1141 ctgctgtttc ccccgcagcc ggaccggtca attcaaccgt caccataacc ggaagcaatt
     1201 tcagcctgac cccatccgcc aatgccgttt ggttcgggtc cgttcgggtg cccgtcactg
     1261 ctgcatcggc gaccagcctg acccttacgg tccccacagg caccagctat gaaccgatca
     1321 tcgtcacgac cggcggtctt acctccgcgc cgttcgcacc ttttatcacc actttttccg
     1381 ataccggaca gtttaccccg gcggcctttt ccacccgtac cgatgttccg accggctcag
     1441 gcccgcaatg gatctgtaac gcggaccttg atggcgacgg caaaccggat ataatcgttg
     1501 caaacggtga ttcgaatact gtcaccgttt accacaataa cagtaccccg ggaacagtct
     1561 ccttcgccga ggcaggttcc tacatgatgg gcaccagcgg gtacccgatc ggcgtcgtcg
     1621 ccggcgacct tgacggcgac ggcaaaccgg acctggtcat ctctaactac tatacccaaa
     1681 cgctgactgt ctacctcaac gccagcacgc ccggaaatat cgtcatggac tctgtcctga
     1741 gcgtcccctc ggccaactat attcttggcg ccgccatagc cgacctgaac ggcgacggca
     1801 aaccggaggt tattgtagcg agccagggca gcaacatgtt ggccgtctac acgaacagca
     1861 gtatcatcgg gaaaatttcc ttttcgaatg agacgcctat catgtcgcct tcaggcggct
     1921 cgccgttcaa agtggtggtt ggcgacctcg acggagatgg gaaaccggac ctcgctgcgg
     1981 ccaactccta tctgggaacg gtttcagtct atctgaatac ctccccggcc gaaggtccga
     2041 tctccttcgc ggcaaacgtg gacttcgcca ctggcaattt ccccgaaggc atggctatcg
     2101 gggatcttga cggcgacggc aaacccgata tggtcgtcgc caacaatacg gataacaccc
     2161 tatctctctt acgcaatacc agtacgcccg gcaacctcag ttttgcgtcc cagctgaccg
     2221 tcaattcagg ttacgccgcc tatgatatcg cattggggga cttcgacggc gacgggaagc
     2281 ctgacatcgc cgtcgtagat caatataata acaccgtatc gatccaccgg aacgtcagca
     2341 ccccgggggc gattgccgtg agcccgaatg tggactacaa taccggcaac atcccatacg
     2401 ccattagcag tgccgacttt gacggtgacg gaaaaccgga ccttgcgacg tgcaacgacg
     2461 ccgacaatac gttcactgtg ctacgcaata aagggtctaa cgagtcggcg ataacctctt
     2521 tcagcccggc gacgggcacc cagggcaccg tcgtaaccgt tgtcggtgtc aacctgaccg
     2581 gcgccaccag cgtcagcttc ggcgactccg cggccagctc gtttaccgtc gtctctccgg
     2641 atacgattac cgcggttgtc ggcaccggcg ccacgggcgc ggtaaccgtt gtcacagcgg
     2701 gcggatccgc ctcccttgcc ggttttgtct acggcacgca accggtcatc agcggcttca
     2761 gcccggattc ggccgcgcag ggcgcttccg ttaccatcac cgggaaaggc tttacgggaa
     2821 cgaactcagt cctttttggc ggtacgcctg cagtctcctt cacggtttcc aacgactcga
     2881 ccattatggc taccgtcgga gccgggacca caggcgctat tacggtctcc ggaccccagg
     2941 gctctgcgac ggccggaggc ttcgtctacc tggtggtcgc tccgccgccc atcgtcctgc
     3001 tttc
//
LOCUS       DATGKE010000263         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1472100, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000263 DATGKE010000000
VERSION     DATGKE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472100"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VKU83_04510"
     CDS             complement(<1..424)
                     /locus_tag="VKU83_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92841.1"
                     /translation="MKRIFFIALPMIALTTMLWTSAGAQDKTQSSGDDDDWSMSNFPD
                     NPGLWSAIIHGDKVRIEFGGLHWSSSATFALSELGTLPTGKAGAFTVKRGAGTVTFNG
                     SFDGNRGHGTYAFEQDPAFRSYLAQEGFPDVSDNLMIHL"
     gene            complement(507..2153)
                     /locus_tag="VKU83_04515"
     CDS             complement(507..2153)
                     /locus_tag="VKU83_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLI92842.1"
                     /translation="MTWIPETFIRALCWTLLHSLWQGLILAVVAGIVMVVTKRARSAT
                     RYTALSLLVLAFLAVSTCTFIRESIAITPGVHALSQSPAISAATPMAGPGAETAPAQG
                     MTVIGQLAQYFNRHASAVVVVWFIVFLARFVNLLSGLVYAQRIRHYQTNPVGEEWQER
                     MATLLKRLRITAPVELLESALVKVPAVVGWLKPAILVPVGMLAQLPPGQVESILLHEL
                     AHITRRDYLFNLLQHLMDTLFFFNPALLWVSSLIRQERENCCDDLAIREMRSRRKLVQ
                     ALVSFHEYQQSSRTYALGFAGQQNSLLKRVERIVHKKNHSLNAGERALLMGGIMLLCG
                     AFITINGTARVAGTAANKHVISAAATKPGMPGKSAAATTGRGGTATMAAKPAARSMAV
                     ARIRSVLDTTPAGAGNSPAGTMTRADVDLIIRAREHGVSTEFLSELKNMGYTDTLEQA
                     IRLKDHGVSIDFLQGLDKNGYGHIPLEQAVRLVDHGVSISFIQRVRASGFPDLTLDQA
                     VTLVDHGVSSDFIEKWRKKTGSKLELRDYIRLQDAGVQPD"
     gene            complement(2165..2563)
                     /locus_tag="VKU83_04520"
     CDS             complement(2165..2563)
                     /locus_tag="VKU83_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLI92843.1"
                     /translation="MNKESNVEMAEPTRAQLEILQILWKHGPSTVRFVNDQLNQQKQS
                     LSYTSTLKLMQIMHEKGMLQRDESSMTHVYSTRLEESKTKGAVLRKFVDSMYNGSVKS
                     LMLELLGNEQTTSKHWDKIKDLLEKLDEND"
     gene            2700..3143
                     /locus_tag="VKU83_04525"
     CDS             2700..3143
                     /locus_tag="VKU83_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92844.1"
                     /translation="MTPISIIVRKNLTLKLHGFSGIAAGQDWAATGKQLMDRMWKEVR
                     GLRLPNKGLNIWVYEPDNCLFTGVELTSAPPENSALEEKMIILTRYARFAHVGPYDTI
                     KASHTIARQEMTRIGIQSALPYIEIYGHHQDDPALQETELIWSIK"
BASE COUNT          697 a          920 c          852 g          712 t
ORIGIN      
        1 atagatggat catcaggttg tcgctgacat ccggaaaacc ctcctgtgcc aggtaggatc
       61 ggaacgcggg gtcctgctcg aaagcgtacg tgccatggcc acggtttccg tcgaaggaac
      121 cgttgaaggt tacggtgccg gctccacgct tgacggtaaa ggccccggct tttccggttg
      181 gcagggttcc caattcggat aaggcaaacg tggcactgga gctccagtgc aggccgccaa
      241 actcaatccg caccttatcc ccgtggataa tggcgctcca caggccaggg ttatcgggaa
      301 aattactcat ggaccagtcg tcgtcgtcac ccgaactttg cgttttgtct tgagcgccgg
      361 cactggtcca gagcatcgta gtcagcgcga tcatcggaag cgcaataaag aatatccgtt
      421 tcatgatata ggtgtttttt atgaaaaagc ttgtccgggc cgtcggatga cagcgtttgg
      481 aatttctttt ctgtgttgag aaatgcttaa tcaggctgga cacccgcgtc ctgcagcctg
      541 atatagtcgc gcagctccag cttcgacccc gttttcttcc tccatttctc gatgaagtcg
      601 gagctgacgc catggtcgac cagtgtcacg gcctgatcga gcgtcaaatc cgggaaacca
      661 ctggcacgga cccgttggat aaaggaaatc gaaacgccat gatccacgag ccgcaccgcc
      721 tgctcaagcg ggatatggcc gtaaccattt ttatcaagac cctgaaggaa gtcaatactg
      781 acgccgtgat ctttgagtcg aatggcttgt tcgagcgtgt ccgtatagcc catattcttc
      841 agttcgctaa ggaactccgt gctgacgcca tgctcccgcg ccctgataat caaatcgaca
      901 tccgccctgg tcatggtccc tgccggcgag ttgccggctc ccgccggcgt ggtgtcaagg
      961 accgaccgta tccgtgctac cgccattgac cgtgccgcag gcttggcggc catcgtagcc
     1021 gtcccgcccc ttccagttgt ggcggcggcg gatttcccgg gcatcccagg ctttgttgca
     1081 gctgccgata tcacgtgctt gttggccgcg gttcccgcca cgcgggcagt accattgatc
     1141 gtgataaaag ccccacaaag caacatgatg ccgcccatca gcagggcccg ctcacccgcg
     1201 ttcaaagagt gattcttttt gtggacaatc cgctccacac gcttcagcag gctgttctgc
     1261 tgacccgcaa acccgagggc gtaggtgcgg gaggactgct ggtactcgtg aaaagaaacg
     1321 agcgcctgaa ccagcttgcg ccggcttcgc atttcccgga tcgccaagtc atcacaacaa
     1381 ttttcccgtt cctgccggat gagggacgac acccagagca gggccggatt gaaaaagaac
     1441 aacgtatcca tgaggtgttg caggagatta aagaggtagt cgcgtctggt gatatgcgcc
     1501 agctcgtgca acaggatcga ctccacctgc ccggggggca gttgggccag catgcccact
     1561 gggacgagga tcgctggttt tagccagccg actaccgcgg gcaccttgac cagggccgat
     1621 tccagcagtt cgaccggggc ggtgatccgc agccgtttta aaagtgtagc cattctttcc
     1681 tgccattcct cgcccacggg gttggtttga taatggcgga tgcgctgcgc atagacgagt
     1741 ccggaaagca ggttcacaaa cctcgccagg aacacgatga accagaccac gactactgcc
     1801 gacgcgtgac gattgaaata ttgcgccaat tgcccgatta cagtcattcc ctgcgccggc
     1861 gccgtttcag cgccgggacc ggccataggg gtggccgccg atatggccgg actttggctt
     1921 agcgcgtgca cgcccggtgt tatggcgatg gactcccgga taaaagtaca ggtgctaacc
     1981 gccaggaatg ccagcaccaa caggcttagg gcagtgtacc gcgttgccga ccgggcccgt
     2041 ttcgtgacga ccatgactat cccggccacg acggccagga tcaacccttg ccagagcgag
     2101 tgcaggaggg tccagcacag cgcgcgaatg aacgtttcgg gaatccaggt catatagatt
     2161 gcttttagtc gttttcgtcc aatttctcca acaggtcctt gatcttatcc caatgtttgc
     2221 tggtcgtctg ttcgttcccc aggagctcga gcatcaggct cttcaccgat ccgttataca
     2281 tcgaatccac aaatttcctg agcacggctc ccttcgtctt actctcttca agccgcgtgc
     2341 tgtacacatg ggtcatactg ctttcgtccc gctgcagcat tcccttttcg tgcatgatct
     2401 gcatcagctt caacgtagag gtgtagctca gggactgttt ttgctggttc agctggtcat
     2461 tgacaaaacg gacggtcgac ggcccatgct tccataaaat ctgaaggatc tccagctgtg
     2521 cccgggtcgg ctccgccatt tccacgttgc tttccttatt catcggccaa agatataatt
     2581 ttaaacgtac gaacaaggtt ataggtatat gttaacaaag ttttaacaat ccgcgcaccg
     2641 gcggtttctg ctgcaccagc cgggcaccac agtcattgcg atattccgta acttcatgga
     2701 tgacccctat ttccattatt gtcagaaaga atctgacgct caagctccat ggattctccg
     2761 gaatcgccgc cggtcaggac tgggccgcaa ccggcaagca gctcatggac cggatgtgga
     2821 aagaagtgcg tggcctgcgc ctccccaaca aagggctcaa catttgggtc tacgagccgg
     2881 ataactgcct cttcaccggc gttgaattga ccagcgcgcc acctgaaaac agcgctctgg
     2941 aggagaaaat gatcatcctg acccgatatg ccagatttgc acacgtcggg ccatacgata
     3001 cgatcaaagc aagccatacc attgccaggc aggaaatgac aagaataggc atccagtccg
     3061 ctctacctta tatcgaaatc tacggccacc accaggatga ccccgcactg caggagaccg
     3121 agttaatctg gagtattaaa tgacggtggt gctggtttcc gccgtaactt ccggtcatta
     3181 a
//
LOCUS       DATGKE010000264         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_196714, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000264 DATGKE010000000
VERSION     DATGKE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196714"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VKU83_04530"
     CDS             complement(<1..793)
                     /locus_tag="VKU83_04530"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92845.1"
                     /translation="MTREALLGSYIHSPRLFQLADRLLLSSPQRVACRNLQGSSPAFL
                     ISAVFQQETTKQLNHLVVCEDAEAAAYLHNTVESLTEAIDLFYFPSSFKNNKNFKLLN
                     SSHVMLRTEALTRLAAGRGTGKKVIITYPEALFEKVVLPSTLSGNIIAIKAGDALDLN
                     GLLETLVNKGFERTDFVYEPGQFALRGGILDIYSFGNEKPYRIELFGNDVDSIRIFDP
                     ETQMSERRLLQVNIIPNVDNRPDSGDKISLLQFLPENTVVWLQDWG"
     gene            complement(899..1402)
                     /locus_tag="VKU83_04535"
     CDS             complement(899..1402)
                     /locus_tag="VKU83_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI92846.1"
                     /translation="MDFLALNMALCLTLISGPVTPQRGFQRSGIEGRLYLIGGNRMPG
                     PPRRNNDTAHHDNTSRRPAREGPGIKGTICVFELTNDRQVTRKGTSPYCEAVHTKLIR
                     QVDTDDKGNFRIPLPPGTYSVFTRKNNLYYASRRDEKNNIAPVTVVPGKWTRVDYSVE
                     SDQKAYY"
     gene            1443..>4746
                     /locus_tag="VKU83_04540"
     CDS             1443..>4746
                     /locus_tag="VKU83_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLI92847.1"
                     /translation="MTRRDPSLLLLPAALCILVLSGLTSHAQVPVTDIPTLKRTFQPI
                     EAKILLEQQQLTRTALEKGWPLMIPGKKGQLLYLHGIDEFGRPRYVKTVDNIISAATI
                     GTNQLWTQLGLTGSSANVKGKIAIWDEGLVRPTHVELVGRVIQVDNATTLSDHSTHVS
                     GTLIAAGVNPAAKGMANGEQTLQCYDFNGDVQEMDNAAKQGLLVSNHSYATVTGWNPD
                     GSNPNQWDWFGNPGDTVDINFGLYDKETQTWDSIAYSNPFYLIFKAGGNNRGDTGPPA
                     GTNYFRYNAQGTLYNAGARPAFGTSASISSNAGYMTIATYGNAKNIITLGAVQPIPGG
                     YSKPSDVVLAGFSSVGPTGDGRIKPDLVADGVNVLSSISTSDNAYDIYSGTSMATPAA
                     CGSALLLQEYYSKLHTVFMRAATLKALLIHTADEAGPYPGPDYVYGWGLADLVKASSV
                     ISSDNSDHVQQIHESSLTQGTKDSETWNIVASGKMPVSATICWTDPAAAPPNIPANEH
                     NFKDNAIKLVNDLDLRIKDNVTGTVYMPWTLNPLNPGAAAIKSDNIRDNVEKVEVGDS
                     LVPGRSYTITVTHKNTLARGTQNYSLVVSGAGGTAVCTSGSTGGGADITQVAVSNLNN
                     SVAANASCPGYVDYTSVSAANLPVGQSATISVTTSSCNATNNNRAVAVYIDYNNDGTF
                     ESNEQVYLSAAGPAGTVSGSFTVPGTATVGSTALMRVITEETNSPNVISPCGSYANGE
                     TQDYRVSFVNPTNDVGISDLEYPSSNTCPNDSTLVAVRIHNYGSVAQSAVPVTTIVTQ
                     NSTVIGSFSTVCRDSIPANSDVVFTFSGSSLQLTPGASYTFTSYTALGSDLNPANDSN
                     VTTVTANAAGASITGSATACGPNATSVQLKANASGDDVVLWYDRPTAGTPIAAGSNTT
                     TRDVAKTYYVAVNDLTAKGGSLSALQLAPSGASPGFYYPFAGQFMQFTTSVPLTIQSA
                     RLYVAHSGTVVFTLASLATFTRSEYSYYPIDSVSINVYATNSNPQAGRVNVGAGQMTD
                     TGAIYYLNLPIPNPGSYILIAECYNNANLFLNSNIPSNPYPISIPGVFSVTGNSNKYN
                     PNNSSDSTFDRGF"
BASE COUNT         1091 a         1371 c         1295 g          989 t
ORIGIN      
        1 agccccagtc ctgcagccag acgactgtat tctccggcag aaattgaagc agggagatct
       61 tgtccccgga atccggccgg ttgtccacgt tgggtatgat gttgacctgc aggagtcgcc
      121 gctcgctcat ctgggtctcg ggatcgaaga tgcggatcga gtccacgtcg ttcccgaaga
      181 gctcgatgcg gtaaggcttc tcgttaccga atgagtagat atccaggatg cccccgcgca
      241 acgcgaattg gcccggttca tagacgaaat cggttcgctc gaagccctta ttgacaaggg
      301 tctcgaggag accgttcagg tcaagggcat cgcctgcttt gatggcaata atattcccgg
      361 aaagggtgga aggaaggacg accttttcga aaagggcctc cgggtaagtg ataatgacct
      421 ttttcccggt tccccggccg gctgccagcc gcgtcaacgc ctccgtccgc agcatcacgt
      481 gactactgtt gagcagcttg aaattcttgt tatttttaaa ggacgacggg aaataaaaga
      541 ggtcgatggc ttcggtgagg ctttcaacgg tattgtggag ataggcggcg gcctccgcgt
      601 cttcgcagac cacgaggtga ttgagctgtt ttgtcgtttc ctgttgaaag acagcgctga
      661 tcagaaatgc cggggaactt ccctggagat tccggcacgc gacccgctgc ggcgaggaca
      721 ataagagcct gtccgccaac tggaaaaggc ggggtgaatg tatgtaagat ccaagaagcg
      781 cctcacgagt catttccgga tgcaaatata cacaatgtat gcgagatcgc ccttccgccg
      841 gcgtcgcgcg gccgcttttc gcccttaagc aaccgatccg cacaaattca cgtaaatcct
      901 aataatacgc tttctgatcg gattctacgc tgtaatcaac ccgggtccac ttaccaggaa
      961 ccactgttac cggagcgatg ttgttctttt catcccgccg gctcgcatag tacagattgt
     1021 ttttcctggt aaatacagag taggttcccg gcggaagtgg gatcctgaaa tttcctttgt
     1081 catcggtatc gacctggcgg atcagctttg tgtgcacagc ttcacaatag ggggaagtcc
     1141 ctttccgggt cacctggcgg tcgttggtca gctcaaaaac acagatcgta cccttgatcc
     1201 cgggcccctc ccgggcgggg cgtcgcgagg tattgtcatg atgggcagta tcgttgtttc
     1261 gtcgcggcgg cccgggcatc cggttccccc cgatcaggta aaggcggcct tcgatacccg
     1321 accgctggaa cccgcgctgg ggggtcacgg gacctgaaat caaggtcagg cataaggcca
     1381 tatttaaagc taaaaagtcc ataaaaactg taattttaga aaaagttata caaaccacgc
     1441 ttatgaccag aagggacccg tccttacttc tactacccgc agccctatgc attctcgtac
     1501 tttccggtct aacgtcgcat gcgcaggtgc ctgtcacaga tatcccaacc ttaaaacgaa
     1561 cctttcagcc gatcgaggct aaaattctcc tggaacagca gcagctgacc cggaccgccc
     1621 tcgaaaaagg ctggccgctg atgatccctg gaaaaaaagg ccagctgctc tacctgcacg
     1681 gcatcgacga attcggacgg ccacgttacg tgaaaaccgt cgataatatt atttccgcgg
     1741 cgaccatcgg tacgaaccag ctctggacac aattgggact cacgggctct tccgccaatg
     1801 tcaaaggtaa gatcgcgatt tgggatgaag gcctggtccg gccgacgcat gtcgagctgg
     1861 tcgggcgcgt gatccaggtg gacaacgcca caacgctcag cgatcactcg acgcatgttt
     1921 ccgggaccct gatcgctgcc ggcgtcaatc ccgcggccaa agggatggca aacggcgagc
     1981 agacgctgca atgctacgat ttcaacggcg acgtccagga aatggacaac gccgccaagc
     2041 agggtttgct ggtctcgaat cactcttatg caaccgtcac cggctggaat ccggacggga
     2101 gcaatcccaa ccagtgggat tggtttggca atccggggga cacggtcgac atcaatttcg
     2161 gcctttacga caaggagacc cagacatggg attcgatcgc ctacagtaat cctttttatc
     2221 tcattttcaa ggccgggggt aataatcgtg gggataccgg gccccctgcc ggcaccaact
     2281 acttccggta caacgcccag ggtacccttt acaacgccgg cgcgcgcccc gcgttcggca
     2341 ccagcgcctc gatcagcagc aatgccggat atatgacgat cgcgacctat ggcaatgcca
     2401 agaatatcat taccctcggc gcggtgcaac ccattcccgg cgggtattcc aaaccttcgg
     2461 atgtcgtgct ggcgggattt tcgagcgtcg gcccaaccgg cgatggccgg atcaaaccgg
     2521 acctggtggc ggacggcgtc aacgtactgt ccagtatttc cacctcggac aacgcctatg
     2581 acatttacag cggaacttcc atggcgacgc ccgcggcatg tggctctgcg ctcttgttgc
     2641 aggaatatta ttcgaagctc cacacggtct tcatgcgagc tgcgacattg aaggcgctgt
     2701 tgatccatac ggccgacgaa gccgggcctt atcctggtcc cgattatgta tacggctggg
     2761 ggctggcgga tctggtcaag gcgtcctcgg tcatcagctc cgacaactcg gatcacgtgc
     2821 agcagatcca tgaaagcagt ttgacccagg gtacaaagga tagcgaaacc tggaacatcg
     2881 tcgcctcggg aaagatgccg gtaagcgcca cgatatgttg gaccgatccg gcggcggcgc
     2941 cgccgaatat tccggcgaac gagcataatt tcaaggacaa cgcaatcaaa ttggtcaacg
     3001 atctcgatct gcggatcaag gacaatgtga ccgggacggt ctatatgcca tggacactga
     3061 atcccctcaa tccgggcgcc gcggccatca agtctgataa tatccgggac aatgtcgaaa
     3121 aggtggaggt tggagacagc ctcgttcccg gcaggagtta taccatcact gttacgcaca
     3181 agaatacgct ggcgcgcgga acgcagaatt actcgctcgt cgtcagcggg gcaggcggca
     3241 cagccgtttg cacgtccgga tcgaccggcg gcggagcaga cattacgcag gttgccgtga
     3301 gcaacctgaa taattccgtt gccgcgaacg caagctgccc gggatacgtc gattatacgt
     3361 ccgtgtccgc agccaatctg cccgtcggcc agtcggcgac tatttcggtc accacgagca
     3421 gctgcaacgc aacaaacaat aaccgggcgg ttgccgtgta tatcgactat aacaatgacg
     3481 gtaccttcga aagcaatgaa caggtttacc tgagcgctgc gggccctgcg ggcaccgtta
     3541 gcggcagctt caccgttcca gggaccgcga cagtcggcag caccgccctg atgcgcgtta
     3601 ttaccgaaga gacgaacagt cccaatgtca tttcaccttg tggcagctat gcgaacggcg
     3661 agacgcagga ctatcgggtc agcttcgtca atcccaccaa cgatgtgggg atatccgacc
     3721 tggagtaccc gagctcgaat acctgtccca acgattccac cctggtcgca gtcaggatcc
     3781 ataactacgg cagcgtcgcg caaagcgctg ttcccgtgac cacgatcgtt acgcagaaca
     3841 gcacggtcat cggcagtttc agtacggtct gccgcgatag cattcccgcg aacagcgacg
     3901 tggtatttac atttagcggg tcgagcctgc agctcacgcc gggcgccagt tataccttca
     3961 ccagctatac ggcgctggga agcgatctga atccggccaa cgacagcaat gtgactactg
     4021 tcactgcgaa tgccgcgggc gcatccataa cggggtcggc tacagcctgc ggtccaaatg
     4081 ccacatcggt tcagctgaag gcgaatgcca gcggcgatga tgtcgtcctg tggtacgaca
     4141 ggccgacagc cgggacacct atcgctgcgg gcagcaatac caccacccgc gacgtcgcca
     4201 agacctatta cgtcgcagtg aacgacctga ccgccaaggg cggatcactg agcgcgctgc
     4261 aattggcgcc gtctggggcc agccccggtt tttattatcc gtttgccggt cagttcatgc
     4321 aattcacgac gagcgtcccg ctgaccatcc aaagcgcacg gttgtacgtg gcgcattccg
     4381 gcactgttgt ctttacgctc gcctccctgg cgaccttcac ccggtcagaa tattcgtatt
     4441 acccgatcga cagcgtgagt atcaatgtct acgcgacaaa ttccaaccct caggcaggca
     4501 gggttaatgt aggagccggc cagatgacag acaccggagc catctattac ctgaatctcc
     4561 cgatacccaa tcccggaagt tatatcctga tagcggagtg ctataacaat gcgaatttgt
     4621 tcctcaattc gaacattcct tcgaacccat atcctatcag cattccaggc gtattttcgg
     4681 taaccggcaa ttcgaacaag tacaatccga acaattcctc ggacagcaca ttcgatcgcg
     4741 gatttt
//
LOCUS       DATGKE010000265         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_329989, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000265 DATGKE010000000
VERSION     DATGKE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329989"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VKU83_04545"
     CDS             complement(<1..1089)
                     /locus_tag="VKU83_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI92848.1"
                     /translation="MGRQNDNITTGDDYMLYPVPQKDGWHFTVWAPEKKRMMLQLLSR
                     DTGIAMQKDRFGYFSAVVEAAAAGDRYYYLPDGGKQQPDPASQFQPEGVHGPSQLVDH
                     RQHAWQDAAWRGRPLSSLILYEIHVGSFTPDGTFDAIIPRLDDLAALGITAIELMPVA
                     EFPGERNWGYDGVFLYAVQHNYGGPAGLKRLVDACHRRNLAVILDVVYNHVGSEGNCL
                     NSFGPYFSATYQTPWGKALNFDGPWSDGVRRFVIGNVLSWARHYHLDGLRLDAVHEIY
                     DRNAVTIWHELDAAVRQWETRAGRPFFLIAESDLNSPAVVGSPAAGGRGFDAQWLDDF
                     HHALYVLLDRKGQRNYEDYGDLQQLAKAY"
     gene            complement(1094..>2117)
                     /locus_tag="VKU83_04550"
     CDS             complement(1094..>2117)
                     /locus_tag="VKU83_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI92849.1"
                     /translation="RVDAMWHLVKDLQWRDNPHNPEYDDSMATYNQLLPVYSTDQPEM
                     HGIVARMRTLLDSYSQRLMIAEIYLPIHRLVAYYGINDSEAHLPFNFLLISLPWEARQ
                     IAAAIDEYEGALPGDAWPNWVVGNHDQPRITSRVGQAQAMVAAILLLTLRGTPTLYYG
                     DEIGMRDVPIPASEVQDPQGLNMPEKNLSRDPERTPMLWDDSQNAGFTEGKPWLRLDR
                     AWRRVNVESQERNVYSSFALYRRLIQLRQGEQSLKTGAYRPVYSDGQQIAYIRQAEGH
                     PAFLVILNLTHRPCYFDRAGIRFRGVIVLDIFPEMEHCAVEDTVELSGDEGLVIRLEE
                     WKAVKS"
BASE COUNT          422 a          620 c          650 g          425 t
ORIGIN      
        1 atacgccttc gccagctgct gaaggtcgcc ataatcttca taattccgtt gccctttccg
       61 gtccagcagc acgtaaaggg cgtgatggaa atcatccagc cactgcgcat cgaagccccg
      121 gccgccggcc gccggcgacc cgacgacggc aggtgaattg aggtcgctct cggcgataag
      181 aaaaaaaggc cttcccgcac gggtctccca ttgccggact gcggcatcga gctcatgcca
      241 gatcgtgacg gcattgcggt cataaatctc gtggacggca tccagccgca gcccgtcgag
      301 atggtaatgg cgcgcccagg aaagcacgtt gccgatgacg aaacggcgga cgccgtcgga
      361 ccagggacca tcgaaattca gggcttttcc ccatggcgtc tggtaggtgg ccgagaaata
      421 ggggccaaag ctgttgaggc aattgccttc gctgccgacg tggttgtaaa ccacgtcgag
      481 aatgaccgca aggttgcggc ggtggcacgc atcgaccagt cttttcagtc ccgccggacc
      541 gccatagtta tgttgcacgg cgtataggaa aacgccgtcg taaccccagt tccgttcccc
      601 ggggaactcg gcaaccggca tgagttcgat ggcggtaatg cccaacgcgg cgaggtcatc
      661 cagacggggg atgatggcgt cgaaggtgcc gtccggcgta aaactgccga cgtggatctc
      721 gtaaaggatg agcgatgaaa gcggtcttcc ccgccaggca gcgtcctgcc acgcatgttg
      781 ccggtggtcg accagttgtg acggcccgtg tactccttcc ggctggaact ggcttgccgg
      841 atccggttgc tgcttgccgc catcgggcag gtagtaatac cggtcgccgg ccgccgcggc
      901 ttccacaacg gcggaaaaat agccgaaccg gtctttctgc atggcgatcc cggtgtcgcg
      961 ggacaacagc tgcagcatca tgcgcttctt ttcaggcgcc caaacggtaa aatgccagcc
     1021 gtccttttgg ggaacggggt acaacatgta atcatcaccg gtagttatgt tgtcgttttg
     1081 ccggcccatg cgatcaggat tttacagctt tccactcttc gagccgaata acgagaccct
     1141 cgtccccgct gagttcgacg gtatcctcca cggcgcaatg ttccatttcc gggaagatgt
     1201 cgaggacgat cacccccctg aagcggatgc cggcccggtc gaaatagcat ggccggtgcg
     1261 tcaggttgag aatgacgagg aaagccggat ggccttctgc ctgccggata tacgcgatct
     1321 gctggccgtc cgaatatacg ggccggtaag cgcccgtttt aagggactgc tctccctgcc
     1381 gcagttgaat gaggcgacgg tacagggcaa agctcgaata cacatttcgc tcctggcttt
     1441 cgacatttac gcggcgccac gcgcggtcga gacgtagcca gggtttgcct tccgtgaaac
     1501 cggcattctg gctgtcgtcc caaagcatcg gcgtacgctc ggggtcccgg ctaaggttct
     1561 tctccggcat attgagacct tgcgggtcct ggacctcgga cgccggaatc gggacgtccc
     1621 gcatcccgat ctcatcgccg taatacaggg tgggcgtacc ccggagcgtc aggagcagga
     1681 ttgcggcaac cattgcctgt gcctggccga cccggctggt aatgcgcggc tgatcgtggt
     1741 tgcccacgac ccagttgggc caggcgtccc ccggcagggc gccttcgtac tcgtcaatgg
     1801 cagcagcgat ctggcgagcc tcccaaggta gcgagatcag gaggaaattg aaaggcaggt
     1861 gcgcttccga atcattgatt ccataatagg ccacaaggcg atggatgggc agatagatct
     1921 ctgcaatcat tagccgctgc gaataactgt cgagcagcgt ccgcatccgc gcgacgatgc
     1981 cgtgcatttc gggttgatcg gtggaataaa cgggcaagag ctggttatat gtcgccatcg
     2041 agtcgtcgta ttccggattg tgcgggttgt cgcgccattg caggtccttg acgaggtgcc
     2101 acatggcatc cacgcgg
//
LOCUS       DATGKE010000266         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_281950, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000266 DATGKE010000000
VERSION     DATGKE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281950"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VKU83_04555"
     CDS             <1..458
                     /locus_tag="VKU83_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92850.1"
                     /translation="LAASTAEVRQLLNTQTGYMAQSMKNLSDFTANLNKNNDHINRTL
                     ENLEKTSDGLASAKLPETVQNLNGAISEMRATINKINGNNGTLGLLMNDKRLYQNLES
                     TTRSLNTLLDDFRVHPKRYVNISVFGKKDKTGPLSSPLPDSGSAKPVNK"
     gene            467..3106
                     /locus_tag="VKU83_04560"
     CDS             467..3106
                     /locus_tag="VKU83_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HLI92851.1"
                     /translation="MKRWLPACVCWVSALIGLLTLSSGAQAQVVFKSAGSDSIKIVKI
                     LDDEIYHSEQVDSVTTITTLVHHVKIQQDSTLIYCDSLLMNPHEDYIECFGNVHIDDH
                     DSVNIYSDYMKYLVSKKFVHFEKNVRLTDGKGVLTTDALDYDMNLRMGTFDHGGKIVN
                     KESVLTSDRGVYLEDTKDVHFSGNVVLRDPQYDLSADSLLYNTQSQVSTFITKTFVLF
                     KDTTHRTVTTWSGSYDLQNKKASFAKGAGNRPVITDGSQLITGDDVRVDDSTGISTAT
                     GNADYRDTAQGIRLVADYMISNRKKNTFLATRHPVMIIKQEKDSIYVAADTLQSARLV
                     DFEKEQKVLAHQDSLHRLYIDSLEKRSADSLHRVTLARAYKDSLAALTDTLNRDRSDS
                     LSPGAADSLARLGLDSLHRHGLDTLGGLRGDTTAKRAVIKDTTPVERLVYTLSDTSKH
                     FTDRQRKKLEKELSRARSDSARAVAAAVRDSISKKVQARKDSISDAKYALKVKRKAER
                     DSVKAAAAAVKKRQREAIDSVRRAVFAAKARVREIADSIQRRKVLDTSISQALARGDT
                     IRTRRLADSLRKSGWSDSALTQNRLPIPIDSFALRRTQDSLRAVAVQKAYADSIAHLP
                     PEDTTLRYIVGYHHVRIYSDSLQAVSDSLYYSAKDSIFRLFYNPVAWGSGNYQITGDT
                     MYVYTKNKKATRLYVFENALAVNRVARSLYNQLKGTTINCYFLNGEIDYLRAKGNAES
                     IYYVADDNKAFTGANRAHADIIDMIFGPKLDSAGHVALDSAGKPKGKELDRVVLRNDA
                     EGTMIPMRRVVPEDMVLRGFKWLERRRPKSREGIFTSLYTDTEEQSYEEAQKAAEQAP
                     GSQRAPIPTIPIIKPKQQPKRHP"
     gene            3205..3666
                     /locus_tag="VKU83_04565"
     CDS             3205..3666
                     /locus_tag="VKU83_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92852.1"
                     /translation="MRTLFFCLVMLITSGLYNTLSAQDYKMAVGIRFSTAAPTLNNSI
                     SFKYFIDETNAVEALASFGSRFGLGGLYERHQLINGTPAFTWFYGGGGYVGFESGQTW
                     FGPTGVVGLDYKFANAPVNLSLDWKPELDLVPSINFVPDAFGLTARFTFGH"
     gene            complement(3663..>4035)
                     /locus_tag="VKU83_04570"
     CDS             complement(3663..>4035)
                     /locus_tag="VKU83_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92853.1"
                     /translation="SLLRTSGTFDAILNALRYLFHLTGADTRFVDGLPTALIKPLTSA
                     GARGMLVDTMKTIGKDSFAAHLSAILQGSSESTFYVAAVYYGAVAIRNTRYSIGAMLL
                     ADLVSIVSSIFLCYLFFGQLR"
BASE COUNT         1012 a         1158 c         1105 g          760 t
ORIGIN      
        1 acctggccgc ttcaacggcc gaggttcgcc agctcctgaa tacgcaaacg ggctacatgg
       61 cgcagtcgat gaaaaaccta agcgacttca ccgccaacct gaacaaaaat aacgatcaca
      121 tcaaccggac gctggagaac ctggaaaaga caagtgacgg cctggcttcg gcaaagctcc
      181 cggagaccgt ccaaaatctg aacggcgcca tcagcgaaat gcgtgcgacg attaacaaga
      241 tcaatggcaa taatggcacg ctgggcctgc tcatgaacga caaacgcctc taccagaatc
      301 tggagtcgac cacccgcagc ctgaacaccc tgctcgacga tttccgcgtc catccgaagc
      361 gttacgtcaa tatctcggta ttcgggaaaa aagacaagac cggaccgctg agctccccac
      421 tgcccgattc cgggtcggcc aaacctgtca ataaataatc ccgcggatga aaaggtggct
      481 tccggcttgt gtctgttggg tctccgcgtt gatcgggctg ctaacgcttt cttccggtgc
      541 gcaggctcag gtggtgttca aatccgccgg cagcgattcc atcaagatcg tcaagatcct
      601 ggacgacgag atttatcatt ccgagcaggt tgattcagta accaccatca cgacacttgt
      661 ccaccacgtt aaaattcagc aggattccac actgatctat tgtgacagcc tgctgatgaa
      721 tccccatgag gattacatcg aatgttttgg caacgttcat atcgatgacc atgactccgt
      781 gaatatctat tcggactaca tgaagtacct ggtcagtaaa aaattcgtgc actttgaaaa
      841 gaatgtcagg ctcaccgacg ggaagggcgt gcttacgacc gatgcgctgg actacgatat
      901 gaacctgcgc atgggcacgt ttgatcacgg cggcaagatc gtcaacaaag agtccgtgct
      961 caccagcgac cggggggttt atttggagga tacaaaggat gtccacttca gcggcaacgt
     1021 ggtcttgcga gacccgcaat acgatctttc ggccgacagc ctgctctata acacccaaag
     1081 ccaggtctcg acctttatca ccaagacctt tgtgttgttc aaggacacca cccaccgcac
     1141 cgtcaccacc tggtccggga gctacgatct gcagaacaaa aaggcaagct tcgccaaggg
     1201 cgccggcaac cgcccggtca tcacggacgg atcccaactc atcaccggag acgatgtgcg
     1261 cgtcgacgac agcaccggca tcagtaccgc aaccgggaat gccgactacc gggataccgc
     1321 gcagggcatc aggctcgtcg cggattatat gatctccaac aggaaaaaga acacctttct
     1381 ggcaacccgg catccagtga tgatcatcaa gcaggaaaaa gactccatct acgtggctgc
     1441 ggacaccctc caatccgccc ggctcgtcga ttttgaaaaa gaacagaagg tcctggcgca
     1501 ccaggacagc ctgcaccgcc tttacatcga cagcctcgag aagcgttcgg ctgacagcct
     1561 ccaccgcgtg acccttgcca gggcctataa ggactctctt gccgccttga cggacaccct
     1621 gaaccgcgac aggagcgaca gcctgtcccc cggcgctgcc gatagcctgg caaggctcgg
     1681 gctggacagc ctgcaccggc acggcctgga taccctcggc ggactccgtg gcgatacgac
     1741 ggcaaaacga gccgttatta aggacacaac acccgtcgaa cggctggttt atacgctttc
     1801 cgacacatca aaacatttca cggaccggca acggaagaag ctggagaagg agctctccag
     1861 ggcccgctcg gatagcgcca gggccgtggc ggcggcagta agggacagta tttccaaaaa
     1921 ggtccaggca aggaaggata gcatcagcga cgcaaagtac gccctcaaag tcaaacgaaa
     1981 ggcggagcgg gacagtgtca aggccgctgc cgcagccgta aaaaaacgac agcgggaggc
     2041 gatagacagc gtccgcaggg cggtctttgc tgccaaggcc agggtccgcg agatcgcgga
     2101 cagcattcaa cgccgcaaag tcctggacac cagcatcagc caggcgcttg caaggggaga
     2161 taccattcgg accagacggc tcgcagacag cctcaggaaa tcaggttgga gcgacagcgc
     2221 cctgacgcag aaccgcctcc cgataccgat cgatagtttt gcgctccggc gaacgcagga
     2281 tagccttaga gcggtggccg tccagaaggc ttatgctgac agtatcgcac acctgccgcc
     2341 cgaggacacg acactgcggt atatcgtcgg gtaccaccat gtccgcatct actcggattc
     2401 cctccaggcg gtctccgaca gcctgtatta ctcggcaaag gactctatat tcaggctatt
     2461 ctataatccg gtagcctggg gaagcggcaa ctaccagatc accggcgata ccatgtatgt
     2521 gtacacgaag aacaagaagg cgacgcggct gtatgtcttt gagaatgcac tggcggtcaa
     2581 ccgggttgcc cgtagcttat acaaccagct aaaagggaca acgatcaatt gttatttcct
     2641 caacggggag attgactacc tccgcgcaaa aggaaatgcg gaaagcatct actatgttgc
     2701 ggatgacaac aaggcgttta caggcgcgaa ccgcgcgcat gccgacatca tcgatatgat
     2761 cttcggcccc aagctggact ccgcggggca tgtggcgttg gattcggcag gcaaaccgaa
     2821 ggggaaagag ctggaccggg tcgtgttgcg aaacgacgct gaaggaacaa tgatcccgat
     2881 gcgccgggtg gtccccgaag acatggtgct tcgcgggttc aaatggctgg agcgaagaag
     2941 accgaaatcc agggaaggga tctttacgtc cctttatacc gatacggaag aacagagtta
     3001 tgaggaggcg cagaaagcag ccgagcaggc acccggttcc cagcgggcgc cgattccgac
     3061 gatcccgatc atcaaaccca aacagcagcc taaacggcat ccctgacggg atgcggcctg
     3121 gaatgtccta ttttaagaaa gttctaacag gacggactta atacgattgg cactattttc
     3181 gttcaaacag cacaaaccct ccatatgcgg acgctatttt tttgcctggt catgctgatc
     3241 accagcgggc tgtataatac tttatcggcg caggactata aaatggcggt cggtatccgc
     3301 ttcagcaccg cggcgcctac gctgaacaat tccatcagct tcaaatactt catcgatgag
     3361 acgaacgcgg tggaagccct ggcttccttt ggctcgcggt ttggtctcgg cggtctgtac
     3421 gaacgccatc agctgatcaa tggaacaccc gcttttacct ggttctacgg cggcgggggg
     3481 tatgttggat ttgaaagcgg ccagacctgg ttcgggccaa ccggtgtcgt tgggctggat
     3541 tataaattcg cgaacgcccc cgtgaatctt tcactcgact ggaaaccgga gctcgacctc
     3601 gttcccagca tcaattttgt gcccgatgcg tttggcctga cggcacgctt taccttcggt
     3661 cactagcgca gctggccgaa gaataaatag caaaggaaga tcgaactgac aatgcttacc
     3721 aggtcggcga gcagcatggc gccgatggag taccgggtgt tgcggatcgc cacggcgccg
     3781 tagtagactg ccgcgacata aaatgtggat tcggacgagc cctgcaggat agccgaaaga
     3841 tgcgcggcga aactgtcttt cccaatcgtt ttcatggtat ccacgagcat tccccgcgcg
     3901 ccggcactcg tcaacggctt gatcagcgcc gtcggcagcc cgtccacaaa gcgggtatcg
     3961 gcgccggtca gatggaacag atagcgcagc gcgttgagga tcgcatcaaa agtaccgctc
     4021 gtacgcagca agctg
//
LOCUS       DATGKE010000267         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_329956, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000267 DATGKE010000000
VERSION     DATGKE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329956"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..455
                     /locus_tag="VKU83_04575"
     CDS             150..455
                     /locus_tag="VKU83_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLI92854.1"
                     /translation="MKNPIGNLNKVFDSRIRMGVMSILLVNAEVSFNELKQLLELTDG
                     NLASHLTNLEENGFVKVHKGFVGRKTNTTYSITKAGEKAFKDHLQALENIIHGVSGA"
     gene            541..1947
                     /locus_tag="VKU83_04580"
     CDS             541..1947
                     /locus_tag="VKU83_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLI92855.1"
                     /translation="MKIKMILPALTEAKSPFWRPIKYSLFPPLGLATLAAFCSPGDEI
                     DLQDEHVEELQLDDQPQLVIIQVYITNAFRAYRIADHYRKKGAYVCLGGLHVTSLPED
                     AAPHADSIFIGPGEDNFPQFLKDFRQKRPGKIYRQITRDIAAVPPIRRDLIKRNRYLV
                     PNSIVVSRGCPHHCDFCYKDAFFEGGRSFYTQQVDAALSEIDRLPGRHLYFLDDHLLG
                     HQRFASGLFAGMKGMDRVFQGASTIDAILKGSLIEQAADAGLRSIFVGFETLNRNNLV
                     QSNKRQNIGKDYEEAIRRLHSLGIMINGSFVFGLDDDDPEVFERTVDWAVRNSLTTCT
                     FHILTPYPGTRLFASMEAQGRILHRNWDRYDTRQVVYQTVGLSAEQLKRGYDRAYRSF
                     YSWSNILKASLRHDRVKHMIKHFAYAGGWKKFEPMWNFLIKTEGLNKMLPVLEAILSK
                     VGRKSHRGEPVSPFPAIA"
     gene            1978..2352
                     /locus_tag="VKU83_04585"
     CDS             1978..2352
                     /locus_tag="VKU83_04585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLI92856.1"
                     /translation="MQSTSHSFSFRSRVRSLYFAMTGILEFLREEHNARIHLVATIAV
                     GVAAYVARVTYPEAAALAIVVGLVWITEMLNTCVERIMDFVQPEEHPKIKFIKDLAAG
                     AVLAASFTAVVVGLLIFIPKFK"
     gene            2349..3047
                     /locus_tag="VKU83_04590"
     CDS             2349..3047
                     /locus_tag="VKU83_04590"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HLI92857.1"
                     /translation="MRSLPVPSPHRRRWFLFKTPTDENLTVSAAFSCVLVLLRVLYTG
                     RPTFLFMPWNLFLAYVPYFISTWLSQWDRRVHPLVRVAGLALWLLFIPNSPYILTDLY
                     HLADSHRDLRIPEWYDLVLILSFALSGTLIAVMSIRQVERLVTSRARFPGRWLFLFPL
                     MFVNALGVYIGRYLRYNSWDIVTNPVGLATDIAAMIIHPLRHDYAWGMIFSFSILLSL
                     IYSLLANCLPAREE"
     gene            3177..3719
                     /locus_tag="VKU83_04595"
     CDS             3177..3719
                     /locus_tag="VKU83_04595"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLI92858.1"
                     /translation="MAATPATRPGSPQNSRLRKFFIDQLQEIYWAEQKIFQSLSKLED
                     AAHSAELKQAFNSHLQETKNHASRLEKVFQKLGADATARKCPALERIADEAEEIIDET
                     EEGTAVRDVGLIFAAQKAEHYEIAAYGGLVQLARILGFTDAAEVMGVTLAEEKKADSQ
                     LTRIAAGGINIHAKEEMAED"
     gene            complement(3748..>4215)
                     /locus_tag="VKU83_04600"
     CDS             complement(3748..>4215)
                     /locus_tag="VKU83_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92859.1"
                     /translation="LFPKAYQNPQPKDPTYAFQSLAVNLRGFDQNITNGNNDVVINSE
                     LRLPIFTTLFNKPINNAFLRNFSLVQFFDLGTAWNGQYNALKRPQLIISAPQYPYLNV
                     LLKAGGIGPFAGGYGFGVRSTLLGYFLRLDAGWQMNNFFSQKPVLNVSMGVDF"
BASE COUNT          940 a         1163 c         1174 g          938 t
ORIGIN      
        1 cgctatctgg ggatcctgga gataatatta ggttgtatca gtatgtatta tttgcactgg
       61 gggctgtatt tctggacggc tggtttcggt gtcctgcata tcatatacgg actgatcatg
      121 tggtggaagt acgagagggg ggggaatgta tgaagaatcc gatcggtaat ctgaataagg
      181 tattcgatag ccgcatccgg atgggggtga tgagcatcct cctcgtgaat gcggaagtca
      241 gcttcaacga actgaagcag ctgctggaac tcacggatgg gaacctggcc tcgcacctga
      301 ccaatctcga agagaacggc tttgtgaaag tgcacaaggg ctttgtcggg cgaaaaacca
      361 acaccacgta ttcgattacg aaggctgggg agaaggcttt caaggatcat ctccaggcgc
      421 tggaaaacat catccacggg gtgagcggcg cgtgaagcga cggcttagtt gccaaatcaa
      481 gcgcggggaa agccgctttt ttgtgatcca tgcactttga actacaaagt acttttaaat
      541 atgaaaataa aaatgatcct ccccgccctc accgaagcca agagcccgtt ctggcggccg
      601 atcaaatatt cgctttttcc gcccctgggc cttgcgaccc ttgccgcctt ttgttcgccc
      661 ggggatgaga ttgacctgca ggatgaacat gtcgaggaac tgcagctcga cgatcagccg
      721 cagctggtaa tcattcaggt ctatatcacc aatgccttcc gggcttatcg catcgccgac
      781 cattaccgga agaaaggcgc gtacgtctgt ctcggcggtc ttcatgtcac gtcgctgcct
      841 gaagatgccg ccccccatgc cgacagcatt ttcatcggcc cgggcgaaga caacttcccg
      901 caatttttga aagatttccg ccaaaaaagg cccgggaaga tatatagaca gattacgaga
      961 gacattgcgg ctgtcccgcc gatccgccgg gacctcatca aaaggaaccg ctacctcgtc
     1021 cccaattcga tcgtcgtttc gcgtgggtgc ccgcaccact gtgatttctg ctacaaagac
     1081 gccttctttg agggcgggcg gtcattctac acccaacagg tggacgcggc tttatcggag
     1141 atcgaccggc tgccgggcag gcatctgtat tttctcgatg accacctcct cggccaccag
     1201 cggtttgctt ccgggttgtt cgcggggatg aaagggatgg acagggtctt ccagggagct
     1261 tcgaccatcg atgcgatatt aaagggcagc ctgatcgagc aggctgccga tgccgggttg
     1321 cgaagtatct ttgtaggctt tgaaaccctg aaccggaaca acctcgtgca aagcaacaaa
     1381 cgccaaaaca tcggcaagga ttatgaggag gccatccggc gtctccattc gctcgggatc
     1441 atgatcaatg gcagcttcgt cttcggactg gacgacgatg accccgaggt cttcgaaagg
     1501 acggtggact gggcggtcag gaattccctg acaacctgca cctttcacat cctgacgccc
     1561 tatcccggga ccaggctgtt cgccagcatg gaggcgcagg gaaggatcct tcacaggaat
     1621 tgggaccggt acgacacgcg acaggtcgtg tatcagaccg tcggtctgtc cgcggaacag
     1681 ttgaaacggg gctatgaccg ggcctatcgc agcttctact cgtggtccaa tattctgaaa
     1741 gcaagtctcc ggcatgaccg cgtgaagcac atgatcaaac acttcgctta tgccggcggc
     1801 tggaaaaagt tcgagcccat gtggaatttt ctgatcaaga ccgaaggctt gaacaagatg
     1861 ctcccggtat tggaggctat tctttctaaa gtcggccgta aaagtcatcg cggcgaaccc
     1921 gtcagcccgt tccctgccat tgcctgaaga gagaaataaa ctttaaacgt tcgtcatatg
     1981 cagtctactt cgcattcgtt ttcgttccgc agccgggtcc gcagccttta tttcgcgatg
     2041 accggcatcc ttgagttcct ccgggaagaa cacaatgccc gaatccatct cgtcgcgacc
     2101 attgcggtag gcgtcgcggc ctatgttgcc agggtcactt acccggaggc ggcggccctt
     2161 gcgatcgtcg tcggtttggt ctggataacg gagatgctca atacctgtgt ggaacgaata
     2221 atggactttg tgcagcccga agagcatcct aaaataaaat tcataaaaga tcttgccgcc
     2281 ggggctgtcc tggcggccag cttcacggcg gttgttgttg ggcttttaat ctttatacct
     2341 aagttcaaat gagaagtcta cccgttccat cgccgcaccg gcggcggtgg ttcctgttca
     2401 aaacgcccac tgatgaaaac ctgacggttt cggcggcgtt cagttgtgtc ctggttttgt
     2461 tgcgcgtcct gtacacgggc cgcccgacgt tcctgttcat gccctggaac ctgttcctcg
     2521 cgtatgtccc ctatttcatt tccacctggt tgtcgcaatg ggaccgccgg gtccatccgc
     2581 tcgtccgggt tgccggactg gccctttggc tgctgttcat tcccaactcg ccgtacatcc
     2641 tgactgacct ttatcacctc gccgacagcc accgggactt gcgcatcccg gaatggtatg
     2701 acctggtgct gatcctcagt ttcgcgctga gcggcacact gatcgccgtc atgtccatcc
     2761 ggcaggtgga aaggctcgta acatcccggg cccgctttcc cggccgctgg ctctttttat
     2821 tcccgctgat gttcgtcaac gcccttggcg tatatatcgg ccgctacctg aggtataaca
     2881 gctgggatat tgtaaccaat cccgttggcc tggccaccga cattgccgcc atgatcatcc
     2941 atccgctgcg ccacgactat gcctggggga tgatcttttc tttttcgatc ttactgtccc
     3001 tgatctacag cctgctggca aattgcctcc cagcgcgtga ggaatagagg gtcgcgcgca
     3061 cttgcgccgc caatggccgg ttacgacctt cctgttcagg cgggtcgcgg ccttcgcgcc
     3121 gcctcccgga tgcctggcat aagaatttct gcaggctgct aataaaacca cctcttatgg
     3181 cagccacacc cgcgacccgc ccggggtcgc ctcaaaactc aaggttgcgg aaatttttca
     3241 tcgaccaatt gcaggagata tactgggcgg aacagaaaat cttccagtcc ctttcaaagc
     3301 tggaagacgc cgcccattcc gctgaactca agcaggcgtt caacagccac ctccaggaaa
     3361 caaagaatca cgcatcgcgc ctggagaagg tctttcaaaa gcttggtgca gacgcgacgg
     3421 cgagaaaatg ccccgccctg gaaaggatcg ccgatgaggc cgaggagatc attgacgaga
     3481 cggaggaagg tacggctgtc cgggatgtcg gcctcatctt tgccgcacaa aaagccgagc
     3541 attatgagat cgccgcctac ggcggcctgg tgcagcttgc gagaatcctg gggttcacgg
     3601 atgcggctga ggtcatgggg gtgacgctgg cagaagaaaa aaaggcggac agccaactga
     3661 cgcgtatcgc cgcgggcggt attaacattc acgccaaaga ggaaatggcg gaagactgag
     3721 gtgcgcggcg aagcggaagc ggcgcaacta aaagtcgacg cccatcgaga cgttaagcac
     3781 aggcttctga gaaaagaaat tattcatctg ccagccggca tcaagccgga ggaaatatcc
     3841 cagcaacgtg ctgcggaccc caaatccgta ccctcccgca aagggcccga tgccgccggc
     3901 cttcaggagc acgttgaggt agggatattg cggcgcgctg atgatcagtt gggggcgctt
     3961 cagggcgttg tactgcccat tccaggccgt cccaaggtca aagaactgca caagggagaa
     4021 attccgcaaa aacgcattgt tgatcggctt gttgaaaagc gtggtaaaga tcggcaggcg
     4081 cagctcgcta ttgataacga cgtcattatt tccgttggtg atgttttgat cgaacccgcg
     4141 cagattgacg gccagcgact ggaacgcata ggtcggatcc ttcggttgcg gattctgata
     4201 tgctttggga aacag
//
LOCUS       DATGKE010000268         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_225792, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000268 DATGKE010000000
VERSION     DATGKE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225792"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VKU83_04605"
     CDS             <1..389
                     /locus_tag="VKU83_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92860.1"
                     /translation="LSPQIFWFRKMRRNITVTFFMSIIVNIGMWFERFVIIVLSIYRD
                     YIPSSWAIYYRPSIWEVGFYIGTFGLFFTCYFLFSKFFPVIAIAEIRHILKKSNTEFK
                     ATMGHEEAETVHEFAEEYLHHGHLEQ"
     gene            401..991
                     /locus_tag="VKU83_04610"
     CDS             401..991
                     /locus_tag="VKU83_04610"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HLI92861.1"
                     /translation="MSVKKFVVGIFTDEQVLFPAIRKVRSAGYKIHDVYTPMPIHGLD
                     HAMGLRETSLHTAGFIYGLCGTCTALGGIGWIFNSDWPLNYDGKSHFDLAAWIPITFE
                     LTVLFSAVGMFLTFCYLCQMGPFVRKHHFNLRSTDDQFTMVIELTEKSSEAGVIDYLK
                     SVGATGINTQTAETGWWWGTYDRDKTPFEQKGTALV"
     gene            1035..1676
                     /locus_tag="VKU83_04615"
     CDS             1035..1676
                     /locus_tag="VKU83_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLI92862.1"
                     /translation="MKKLIISSLIMVAVTLAVSCSDVQRNPGRIYMPDMTYSREYETY
                     ATTEEMKADLLKRGIHFSNMPVAGTIKRGELLPFLIPKDSVAGDTSNYHAAREVKNPL
                     TSMDTVEAARLYLVNCGICHGPKMDGNGPLYKGGDGPFPAKPADFLGGAQYLAMPEGQ
                     MFYSVTYGKNKMGSYASQLNTKQRWMVIAYIKSKQAAQKPASGGGSDSTAAKQ"
     gene            1718..>2054
                     /locus_tag="VKU83_04620"
     CDS             1718..>2054
                     /locus_tag="VKU83_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92863.1"
                     /translation="MSSISSIREQFQTPTRYKQWSMGLMAVGVLSLIIGFIVYGAKND
                     PSGARFWAALLQNSVYFLLIVNAAMFFYAAHSLAMAGWQLAFSRVTEAVASCVIPLGI
                     ITFVILCCLV"
BASE COUNT          462 a          539 c          578 g          475 t
ORIGIN      
        1 tcctgtcgcc gcagatcttc tggttccgga agatgcggcg caatatcacc gtcactttct
       61 tcatgtcgat catcgttaat atcgggatgt ggttcgaacg cttcgtgatc atcgtgctgt
      121 cgatctaccg cgactatatc ccgagcagct gggccatcta ctaccggccg tcgatctggg
      181 aggtgggttt ctacattgga acattcggct tgttcttcac ctgttatttc ctcttctcga
      241 aatttttccc ggtgatcgcg attgccgaga tcaggcatat cctgaagaaa tcgaatacgg
      301 agttcaaggc tacaatgggc cacgaggagg ctgagacggt gcatgagttt gcggaggagt
      361 atctacatca cgggcattta gagcaataaa agtcattgtt atgtcagtta aaaaatttgt
      421 ggttggaatt ttcacggatg agcaggtgct gttcccggcg atcagaaaag tgcgttcggc
      481 aggttacaag atccacgacg tgtatacgcc gatgccgatc catggcctgg accacgccat
      541 ggggctgcgg gaaacaagcc tccataccgc cgggttcatc tatggccttt gcgggacctg
      601 tacggcgctg gggggcatcg gctggatctt caattccgac tggccgctga actacgacgg
      661 caagtcccat ttcgaccttg ctgcctggat acccatcacc ttcgagctca ccgtgctctt
      721 ctcggctgtg gggatgttcc tcactttctg ctacctctgc cagatggggc ctttcgtgcg
      781 gaagcatcat ttcaacctgc ggtccacgga cgaccagttt acgatggtga tcgaacttac
      841 cgaaaagtcc agcgaggctg gggtcatcga ttacctgaaa agcgtgggtg ccaccggcat
      901 caacacgcag accgccgaga ccggctggtg gtggggaacg tatgatcgcg acaaaacgcc
      961 attcgaacaa aaaggaactg ctttagtcta gtcagccgga cgcgtccggc gctgaacgaa
     1021 ttgaatataa aaagatgaaa aaactgataa tctcctcgtt gatcatggtc gcggtgacgc
     1081 ttgcggtcag ctgcagcgac gtgcagcgta accccggaag gatctatatg ccggacatga
     1141 cgtatagccg ggagtacgag acttatgcca ccacggaaga gatgaaggcg gacctgctga
     1201 agagaggcat tcattttagt aatatgccgg tagccggcac catcaagcgc ggagagctgc
     1261 tgccgttcct gatcccgaag gattcggtgg ccggcgacac gagcaactac catgcggcgc
     1321 gcgaagtgaa gaaccccctg acctcgatgg acacggtgga agcggcccgg ttataccttg
     1381 taaactgcgg tatttgccat ggacccaaga tggacgggaa cggcccactg tacaaaggcg
     1441 gcgacggccc tttcccggcg aaaccggcgg atttccttgg cggggcccaa tatctcgcca
     1501 tgccggaggg ccagatgttc tactccgtta cgtatggcaa gaacaagatg ggcagctatg
     1561 cctcgcagct gaacaccaaa cagcgctgga tggtgatcgc ctacatcaag tcgaagcagg
     1621 cggcccagaa accggcatcc ggcggcggct cggattcaac ggcagcaaaa caataggact
     1681 gcaataaggc ttacagaatc aatttaaatc gttccgaatg tcatccatat catccattag
     1741 agaacagttt caaacgccca cccggtataa acagtggtcc atggggctga tggccgtggg
     1801 cgtgctttcg ctgatcattg gctttatcgt atatggcgct aaaaatgatc cgagcggggc
     1861 tcgtttctgg gctgctcttt tgcagaacag cgtgtatttc ctgctcattg tgaacgcggc
     1921 gatgttcttt tatgcagccc attcgctggc gatggctggc tggcaattgg ctttcagccg
     1981 ggtaacggag gcggtcgcct cctgcgtgat ccctctcggc attatcactt ttgtgatcct
     2041 gtgctgcctg gtct
//
LOCUS       DATGKE010000269         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_4208, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000269 DATGKE010000000
VERSION     DATGKE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4208"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VKU83_04625"
     CDS             <1..306
                     /locus_tag="VKU83_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLI92864.1"
                     /translation="LAMQRLRRSGHPVAAVGRKPATVGDVMVTTDRPAIADVDTVTLY
                     LNPANQKEYYDYILSLRPRRIIFNPGAENPELEALAAANGIDTLEACTLVLLSTGQY"
     gene            395..868
                     /locus_tag="VKU83_04630"
     CDS             395..868
                     /locus_tag="VKU83_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HLI92865.1"
                     /translation="MLWRKFNGDPIELPIIDAVEHAIRRETEMGYRLKVCIGTDSQVK
                     GVETEFATVIVFLREGHGGFMFIHNEKTRHQYSIKERMLVEVAKSIEIAYELCNLFSR
                     YNVDMEVHADINTNPHFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKVVN"
     gene            complement(915..1541)
                     /gene="yihA"
                     /locus_tag="VKU83_04635"
     CDS             complement(915..1541)
                     /gene="yihA"
                     /locus_tag="VKU83_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLI92866.1"
                     /translation="MVISKAVYVISSPDYLHCPKADRPEYAFIGRSNVGKSSLINMLC
                     NNDRLAKTSGTPGKTQLINHFVVNDEWYLVDLPGYGFAKVSQTQRKKWEKMIEDYLRK
                     RSNLTNVYVLIDSRHSPQRIDLAFVDLLGEWQVPFCLVFTKADKESQRTVSANVRAFL
                     GKMRESWQFLPQHFVTSTVKKMGRDKILQQIGEMNKEFGEAEEKETIP"
     gene            1584..>2038
                     /locus_tag="VKU83_04640"
     CDS             1584..>2038
                     /locus_tag="VKU83_04640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI92867.1"
                     /translation="MTDYSHDKVVPFEGSTLGKKQQVAEMFDQIAFRYDFLNHFLSGG
                     IDVYWRKQAIRQLRGIRDSHPSGAAVLDVATGTADMPILISKYLPEARITGVDISTGM
                     LELGRQKVKRSGLEDRITLQTGDSEALPFPDNCFDAVTAAFGVRNFENL"
BASE COUNT          469 a          541 c          558 g          470 t
ORIGIN      
        1 ctggccatgc agcgcctgcg gcgatccggt catccggtag ctgccgtcgg ccgcaagccg
       61 gcgaccgttg gcgacgtaat ggtgactacc gaccggccgg ctattgccga tgtcgatacg
      121 gtgacgctct atctgaatcc cgcaaaccaa aaggagtact acgattatat cctttccttg
      181 cgcccgcggc ggatcatctt caaccccggc gcagagaatc ctgaactgga ggcgctggcc
      241 gccgcaaatg gcatcgacac gctggaagcg tgcacgctcg tcctgctcag caccggccag
      301 tattgacgga agccgccgat taataaaacc ttaacaacta cccggttttg ctaattagga
      361 cgtttttcgg taaattcgaa aaatccttga taatatgcta tggcgaaaat ttaatggtga
      421 cccgatcgag ctcccgatta tcgatgccgt tgaacatgcc atccgccggg aaaccgaaat
      481 gggttaccgg ttgaaggtat gtatcggcac cgattcccag gtgaaagggg tggaaacgga
      541 atttgcaacg gtgatcgttt ttttgcggga aggtcatggc gggttcatgt tcattcacaa
      601 tgaaaagacc cgccaccagt attcgattaa agagcgcatg ctggtggaag tggccaagag
      661 catcgagatt gcctatgagt tgtgcaatct gttcagccgg tataacgtcg acatggaagt
      721 gcatgccgac atcaatacca atcctcattt caagagcaac gacgccctga aagaggcgat
      781 gggctatatc cttggaatgg gattcgcctt caaagcgaaa cccgaagcgt tcgcaagcag
      841 cagctgcgcg aataaagtcg tcaactgacg gcctccgctg tccgccgttc cggaaagatc
      901 gcggcgcccg cgatctaggg aattgtctcc ttttcttcag cctctccgaa ttccttgttc
      961 atctcgccaa tctgctgaag gatcttgtcc cggcccattt tctttacggt actggtcaca
     1021 aaatgctgcg ggaggaattg ccagctctcg cgcatctttc ccaggaacgc cctgacgttg
     1081 gcactgaccg tccgctgact ttccttgtcc gctttcgtaa acaccaggca aaagggcacc
     1141 tgccattctc cgaggaggtc tacgaaggcg aggtcgatcc gttgcgggga gtggcggctg
     1201 tcgatcagaa cgtaaacatt cgtgagattt gacctcttcc gcaggtagtc ttcgatcatc
     1261 ttttcccatt tcttcctttg ggtttgggag actttggcaa agccataacc aggaagatcg
     1321 acgagatacc actcgtcatt caccacaaaa tgattgatca attgcgtttt gcccggcgtc
     1381 cccgaggttt tcgccagtct gtcgttattg cagagcatat tgatcagcga ggatttgccc
     1441 acattgctgc ggccgatgaa agcatattcc ggccggtccg ctttgggaca atgaaggtaa
     1501 tcggggctgc tgatgacata gacggctttg ctgatgacca tacgacggaa tgagggtgta
     1561 agatacgtat ctttgcagcc gccatgaccg attactcaca cgacaaagtc gtccccttcg
     1621 aggggtccac gctggggaaa aagcagcagg tcgccgagat gtttgatcag attgccttca
     1681 ggtatgattt tttaaatcat ttcctgtcgg gaggaatcga cgtgtactgg cggaaacagg
     1741 ccatccggca gctgaggggg atcagggaca gccatccttc gggcgctgct gtcctggatg
     1801 tcgcgacagg aactgcggat atgccgatcc tgatctcgaa atatcttccg gaggcgcgga
     1861 taaccggagt ggatatctcg accggcatgc tggaactagg ccggcaaaag gtcaaacgat
     1921 ccgggctgga agaccggatt accctgcaga ccggcgacag cgaggcacta ccctttccgg
     1981 acaattgttt tgacgccgtc acggccgcat tcggcgtccg gaatttcgag aacctgga
//
LOCUS       DATGKE010000270         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1055266, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000270 DATGKE010000000
VERSION     DATGKE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055266"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VKU83_04645"
     CDS             complement(<1..905)
                     /locus_tag="VKU83_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92868.1"
                     /translation="MQHRCSIWASGLMAIFILAVCACKKTAAPKPIPITPLQTLVNSD
                     TSLTMFHQMLIDANDVGLLKDTTASLLIPTNDVLRQAGYTPVIIDSTGSFVLDEMLRY
                     NYVLAASISPATGAYSSYRTLLGAPLYIEKDSASGQFLLNGTATASGAPVVVGQASVY
                     YLSTLVPLAADSLPSLLQSDSTLSFFAAALAITNLYDSTLLNGSYTVLAPVNAAFIQA
                     GYDSIGAIDSANVDSLIPLVQGQILKGLYLTNSFPVPGPVFDVLGNPVTVGLASGQLQ
                     FSASGNSVPVNWLGGNFISGSNIVV"
     gene            complement(908..1981)
                     /locus_tag="VKU83_04650"
     CDS             complement(908..1981)
                     /locus_tag="VKU83_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HLI92869.1"
                     /translation="MAFRNFKMVGYVMYGRGSFNQLDEMLAPRRKGDAPMVFLLDHFF
                     EKKPLAGRIPLQGKDEIIFADVTHEPKTAYVDRIAGGLKEKFGAISGVIGIGGGSAMD
                     LAKAVSLMMTNPGSSADYQGWDLVRQPGVYKAGIPTLSGTGAEVSRTTVLTGPTKKLG
                     MNSDFTPFDQIVLDPELTANAPVNQRFYTGMDCYIHCIESLQGTFLNEFSKSYGEKAL
                     QLCQEVFVRKQGWDEDSDEKLMMASYAGGMSIAYSQVGVAHAVSYGLSYLLGTKHGVG
                     NCIVFDKLEEFYPEGVREFKKMVEKNGIEIPQHITKGLSDEQFNAMIDVSLVMKPLWE
                     NALGKDWEKIMTRDRLRALYEKL"
     gene            complement(2029..>2412)
                     /locus_tag="VKU83_04655"
     CDS             complement(2029..>2412)
                     /locus_tag="VKU83_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLI92870.1"
                     /translation="YCGKAGCVETVISGPATERFYANLTGKALKLREIAARADEGADE
                     AATETIARLSHFFGKAVSVVTNLLDPDVIVLGGGVGNIAALYTAGRENLGRYIFNNRV
                     EVPLLKPLLGDSAGVFGAAALVAGI"
BASE COUNT          525 a          726 c          657 g          504 t
ORIGIN      
        1 tgaacgacaa tattggaacc ggagataaaa ttgccgccga gccagttgac cgggacagaa
       61 ttgccgctgg cggaaaattg aagctggccg cttgcgaggc cgacggtcac cgggttgccc
      121 aacacatcga aaaccggtcc cgggacggga aagctgttcg tcaggtacag gcccttcagg
      181 atctgaccct ggaccagggg gatcagacta tccacgttgg cggaatcgat cgcgccgatg
      241 ctgtcgtagc cggcctgaat aaacgcggca ttgaccggag caaggaccgt ataactgccg
      301 ttcagtaacg tggaatcata caggttcgtg atggcaaggg ccgccgcaaa aaagctcagc
      361 gtgctgtctg attgtaacag cgagggcaag gaatccgctg ccagcggcac caatgtgcta
      421 aggtagtaaa cggaagcctg gccgaccacc acgggcgcgc cggatgccgt tgccgtgccg
      481 tttaataaga actggccgga tgcgctgtcc ttctcaatgt acaacggcgc tcccagcagg
      541 gtccggtagg agctgtaggc gccggtggcc ggcgaaatcg aagccgccag cacatagttg
      601 taccgcaaca tttcgtcaag cacgaatgat cccgtcgaat cgatgatgac cggggtgtag
      661 cctgcctggc ggagcacgtc gttggtcgga atgagcaggc tcgccgtcgt gtctttcagc
      721 aggccgacgt cgttggcgtc gatcagcatc tggtggaaca tggtcagaga ggtatccgag
      781 ttgaccagcg tctggagcgg ggtgatgggg atgggctttg gcgccgccgt tttcttgcat
      841 gcgcatacgg ccaggatgaa aatggccatg aggccgctgg cccatatcga acaccggtgt
      901 tgcatgttta cagtttctcg taaagcgcgc gaagccggtc ccgggtcatg atcttctccc
      961 agtctttacc cagcgcgttc tcccataacg gcttcataac aagagaaacg tcgatcatcg
     1021 cattgaattg ttcgtcacta agcccttttg taatatgttg cgggatctcg atgccgttct
     1081 tctccaccat tttcttgaac tccctgaccc cctcgggata aaactcctcc agcttatcga
     1141 atacgatgca gtttccgacc ccgtgttttg tccccagcaa gtaagaaagg ccgtagctca
     1201 ccgcatgcgc cacgccgacc tgcgaatagg caatgctcat gccaccggca taggaggcca
     1261 tcatcagttt ctcgtcactg tcctcatccc agccctgttt ccgaacaaaa acctcctggc
     1321 agagctgtag cgccttctcg ccataggact tactgaattc gttgagaaag gtcccctgca
     1381 ggctctcgat acagtggata tagcaatcca tgccggtata aaagcgctgg ttgaccgggg
     1441 cattggcggt gagctcggga tccagcacga tctggtcgaa aggcgtgaaa tccgaattca
     1501 tccccagctt cttggtcggc ccggtcagga ccgtcgtcct tgaaacctcc gcgcccgtcc
     1561 cgctgagggt ggggatcccc gccttataga cccctggctg cctgaccagg tcccagccct
     1621 ggtaatcggc tgaggatcca ggattggtca tcatcagcga gaccgctttg gccaggtcca
     1681 tcgccgaccc gccgccaatt ccgatcaccc cgctgatcgc gccgaatttc tctttcaggc
     1741 cgccggcaat cctgtcgaca taagccgtct tgggttcgtg ggtcacgtcg gcaaagatga
     1801 tctcgtcctt tccctgcaac gggatccggc cggccagggg ttttttttca aaaaaatgat
     1861 ccagcaaaaa caccatgggc gcatcaccct tccggcgcgg cgccagcatc tcatccaact
     1921 gattgaaact gccccgaccg tacataacat agccgaccat cttgaaattc cggaacgcca
     1981 taacgacttg atttgaacgg caaaattacg ggaaaatcgg gcatttcatt atattccggc
     2041 aaccagcgct gccgccccaa atacgcccgc actgtcgccg aggagaggct tgagcagcgg
     2101 cacctccacc cggttgttga aaatataccg gcccagattc tcacgccccg cggtatagag
     2161 cgccgcgata ttgccgacgc cgccacccag gacaatgaca tccggatcca ggaggttggt
     2221 aaccaccgac accgccttgc cgaaaaaatg cgaaagcctc gcgatcgttt ccgtcgctgc
     2281 ctcgtcagcg ccttcatccg cccgggcggc aatttcccgc agctttagcg ctttccccgt
     2341 caggtttgca taaaatctct cggtcgccgg tcccgagatg accgtttcga cacaaccggc
     2401 ttttccgcag ta
//
LOCUS       DATGKE010000271         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_916295, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000271 DATGKE010000000
VERSION     DATGKE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916295"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..510)
                     /locus_tag="VKU83_04660"
     CDS             complement(163..510)
                     /locus_tag="VKU83_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92871.1"
                     /translation="MSQFLAQRDLFDNVLHLNEEEKADPMLVIERFFGDYRLHECRHQ
                     LWTMVETCLTTDNDGFNNASDRGNLLLHYRDLERLLEAAAVLMNKRPRETPRAGLLPG
                     PGGKKPAGKSKPA"
     gene            complement(601..1803)
                     /locus_tag="VKU83_04665"
     CDS             complement(601..1803)
                     /locus_tag="VKU83_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLI92872.1"
                     /translation="MNANLSDARGLFLRHLAQTSPSPLGLQVERAEGVYLYDHSGKRY
                     LDLIGGISVCNTGHRHPKVVTAIKDQVDRYLHLMVYGELIQTPQVRYAALLASHLPPT
                     LNTVYFTNSGTEATEGAMKLAKRLTGRTEMVAFHQSYHGSTQGALSLMGDEYWRNAFR
                     PLLPGIYHERYNSPEALAAITGKTACVVAETVQAERGVYAPDKKWLQQLRDRCDATGA
                     LLILDEIQVGFGRTGTLWGFSQYGIVPDILLLGKALGGGLPLGAFIADQRAMAALTDK
                     PVLGHITTFGGHPLSCAAGLASLEVLLAEGWIGQAGEKEKLFRELLVHPRIRAVRSTG
                     LLMAVEFDSAETNLAVIHACINNGLLTDWFLFAPHCMRIAPPLCIGEAQIRTACDIIL
                     TAISAFFP"
     gene            1886..2425
                     /locus_tag="VKU83_04670"
     CDS             1886..2425
                     /locus_tag="VKU83_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLI92873.1"
                     /translation="MATILPVQGKSPRFGKDCFLAPNATVVGQVEIGNQCSIWFNAIL
                     RGDVGSIHIGDKVNIQDGVVIHATYQKTNVHIGNDVSIGHNAIVHGCTIEDRVLVGMG
                     AIVMDNAVVGRNSIIAAGAVVLEGTRIESGTIYGGVPARKIKDIPPDLISGQIERIAN
                     HYLVYAGWFEASNEAPRNP"
     gene            2789..3124
                     /locus_tag="VKU83_04675"
     CDS             2789..3124
                     /locus_tag="VKU83_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92874.1"
                     /translation="MSPLTLRTDFGCTEAVIDDDCGLKRFYEVANILSDDLDIKFTQK
                     TDDFDSLMWDFLYKGHILTLHYNIYTGISIYPRKFREAPRKDNDAVVEVAKFLEGKLL
                     INTAKRFMS"
     gene            3212..>3532
                     /locus_tag="VKU83_04680"
     CDS             3212..>3532
                     /locus_tag="VKU83_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLI92875.1"
                     /translation="MRQSTPFYTRVAMVLITLISLFYIAVLGKSILAPLLFGLLFAVL
                     LLPLANLLERSLRLPRSIASLLSVVFLILALAGLLYVVGAQIGNLADDWPQFKQQVSS
                     SVTDL"
BASE COUNT          805 a         1001 c          941 g          785 t
ORIGIN      
        1 cgaagcggtg tgatccgctg actgcgcatc caccagacct gggaccatca gcaaaaggaa
       61 aaaggatagc gaaactgctc tcatgaccgc gaatctacaa ataaatggag gcggtgcgaa
      121 acctgcccaa attatgctgc accctcgagc cacccgggaa aatcaagccg gtttggattt
      181 gccggccggt tttttcccgc ctggtcccgg aagcagccct gcgcggggcg tctcccgcgg
      241 ccgtttgttc atcaggacag cagccgcttc cagcaaccgt tccaggtcgc ggtaatgtag
      301 gagcagatta ccccggtccg aagcgttgtt aaaaccatcg ttgtccgttg taaggcaggt
      361 ttccaccatc gtccaaagct gatgccggca ttcgtgcagc cggtaatcgc caaaaaaacg
      421 ctcgatcacc agcatgggat ccgctttctc ctcctcattc aaatggagca cattgtcgaa
      481 aagatcccgc tgcgccagga actgtgacat accgattttt tttaaggtta gaatttgcgg
      541 cgtccgggac gcggacgctt cacttgtccg cagcggggga gaaaaagatg gtcctgcaaa
      601 ttaaggaaaa aaagctgaga tggcagttaa aatgatgtca caggcggtcc tgatctgcgc
      661 ttccccgatg caaagcggtg gggcgatgcg catgcagtgc ggagcaaaga ggaaccagtc
      721 tgtcaataaa ccattattaa tacaggcatg gatgacggcg agattcgtct cggcgctgtc
      781 aaactcgaca gccatcagca ggccggtgct gcgcacggcc ctgatccgcg ggtgaaccag
      841 caattcgcgg aaaagcttct ctttttcccc tgcctggcct atccagcctt cggccagcaa
      901 tacctccagc gatgcgaggc ctgccgcgca ggaaagcgga tggccgccaa acgtcgtgat
      961 atgaccaagg accggcttgt ccgttaacgc cgccatcgcc cgctgatcgg caataaacgc
     1021 gcccaaaggc aaaccgccgc ccagcgcttt tcccaacaat aaaatgtccg gtacgattcc
     1081 atactggctg aaaccccaaa gcgttcccgt ccgcccaaac cccacctgga tctcgtcgag
     1141 gatgagcaag gcgccggttg cgtcacaacg gtcccgcaac tgttgcagcc atttcttgtc
     1201 gggtgcatac accccccttt cggcctgtac cgtttccgcg acgacgcaag cggttttccc
     1261 ggtaattgcg gccagggcct ccggggaatt atagcgttca tgatagatgc ctggcaacag
     1321 cggccggaag gcattgcgcc aatactcgtc acccatgagg ctgagcgcgc cctgggtcga
     1381 cccgtgatag gactggtgga acgcaaccat ttccgtcctt cccgtgaggc gtttcgcgag
     1441 cttcatcgcg ccctcggtcg cctcggtgcc gctgttggta aagtacacgg tgttgagggt
     1501 tggcggcaaa tgcgaggcaa gcaaagcagc ataccggacc tgcggcgtct ggatcagttc
     1561 gccgtagacc atcaggtgca ggtaccggtc gacctgatct ttgatcgccg ttacgacttt
     1621 cgggtgacgg tggccggtat tgcacacgct gatcccaccg atcaggtcga ggtaccgctt
     1681 cccggaatga tcgtaaagat aaacgccttc cgcgcgttcc acctgcaggc ccagtggact
     1741 cggtgaggtt tgggcaaggt gccggagaaa caagccgcgt gcatcggaca aattcgcatt
     1801 catattgtag gcgggctgtc gcccggccga gccggccgga gtcctgctat tagcgcaaag
     1861 ttcgtaaatt tgaaaaaaca gcgatatggc cactatcctt ccggtacaag gcaaatcccc
     1921 gcgttttggc aaagattgct tcctggctcc gaacgcaacc gtggtaggcc aggtagagat
     1981 cggaaatcag tgcagcatct ggttcaacgc catcctccgg ggtgacgttg gcagcattca
     2041 cattggcgac aaggtaaata tacaggatgg cgtcgtcatt cacgccacct atcagaaaac
     2101 caatgtccat atcggaaacg acgtttccat cggacataat gccatcgtcc atggttgcac
     2161 gatcgaagac cgcgttctgg tgggaatggg agcgattgtc atggacaatg cggtggtagg
     2221 caggaattcg atcatcgcgg ccggcgccgt ggtgctggaa ggaacgcgga tcgaatccgg
     2281 cactatttac ggtggcgttc cggccagaaa gatcaaagat atcccccccg atctcatctc
     2341 gggacagatc gagcgcattg cgaatcatta tcttgtctac gccggatggt tcgaggcctc
     2401 caacgaggcc ccccggaacc cctaataact acccatttac atgaggggtt cttacggtta
     2461 aatctacgcc gccggcttgc aaataaggga taatatttgt aggtttaagt acgtttacca
     2521 gtatatccaa tgaaaacgag cccgatacat aagtacccac ggcccaacgc caaaattctc
     2581 ctcgccttcc tggtgctggt gtcgaccggt atcccttcct gcggatggat ccacaatgtt
     2641 ttcggtccga agtacggatg tccgagtgac ggaaaaaatg ttggcgcgga gcggatcctg
     2701 agcggcgaaa aagtaccgaa agctccaaaa ttcaaagcat gacgacagcg atcagatacc
     2761 ctattataaa cgattaaaat ccctagctat gagccctcta accctacgca ccgacttcgg
     2821 ctgcacagag gctgtgatcg acgacgattg cggacttaag cgcttttacg aagttgctaa
     2881 tattctctca gacgacctcg acatcaaatt cacgcaaaag accgacgatt tcgattcgct
     2941 gatgtgggac tttttgtaca agggtcatat cctgaccctg cattacaaca tctacaccgg
     3001 catttcgatc tatccccgca aatttcgcga ggccccccgg aaggataacg atgccgtggt
     3061 ggaagtggca aaattcctcg aagggaagtt gctgatcaac actgccaagc gtttcatgtc
     3121 ctgaccggta tccggcggtt gcttgcctac cttatcaggc cgcatccatt tttgttgatg
     3181 cggccttttt gtttttgcct atcttggcgg tatgagacaa agcacgcctt tttatacgcg
     3241 ggtcgcgatg gtactgatca ccctgatctc acttttctat atcgccgtat tggggaaatc
     3301 catcctcgcc ccgctgttat tcgggctgtt gtttgcggtc ctgctgctgc ccctggccaa
     3361 tcttcttgag cgcagcctgc ggctcccgcg aagcattgct tccttgttat cggtggtgtt
     3421 cctcatcctt gcactggccg gtctcctcta tgttgtcggc gcccagatcg gcaatctggc
     3481 ggacgattgg ccgcagttca aacaacaggt atccagttcg gtaaccgacc tt
//
LOCUS       DATGKE010000272         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1463535, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000272 DATGKE010000000
VERSION     DATGKE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463535"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VKU83_04685"
     CDS             <1..418
                     /locus_tag="VKU83_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92876.1"
                     /translation="QLPFLMQTDLSFFMPGNLIFTLDFTKDETGHITRMVAFKRDQWK
                     KVNKVTPTPAALRQYAGKYQSKDDPDNQISIIARDSALVVKQAWDGKETAYEAVAENF
                     FFNNAASSPLEFVRDANGGIKVVRLFGNNDFIPVGH"
     gene            complement(419..1096)
                     /locus_tag="VKU83_04690"
     CDS             complement(419..1096)
                     /locus_tag="VKU83_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLI92877.1"
                     /translation="MSYTTGAMRALPKEPEAGFNGPHSAAIRIWHWTLFLVMTGSLIT
                     VLLASTVFRTGKNTLLVQNQLQTKGVIISSDQARAVSHAFNDKLWELHKWLGYIICGL
                     VLARFAIELGQPRSEKLNVQLKKVIHAKPNDPGAKAERQHYIQVKSIYLAFYAVLLLM
                     ALTGLGLAFDNIPFLRSIRQPVKQVHSFMQYLIYGFLFIHLAGVIMAELGKHKGLVSG
                     MINGRRE"
     gene            complement(1150..>3048)
                     /locus_tag="VKU83_04695"
     CDS             complement(1150..>3048)
                     /locus_tag="VKU83_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI92878.1"
                     /translation="TTKTPADTAIETLQNVIILSTRTNQLLKNIPIAVQVVGREDIEE
                     GTAQSPANIRELLTELSGTQLQQTSAVSGNVSIRLQGLDGRYTQLLKDGFPLYGGFSG
                     SLGILQVPPLDLHQVEVIKGAGSALYGGDAIAGIINLISRQPDTTPHLDAIENQTQRG
                     ETDLGGFYSKRNRHFGWTVMGTASRQTPVDVNNDGFTDLPQIRQGTIAPTFFWYPDSN
                     TTVRFGINLSAERRTGGDLYAVQHTPDSLHPFRQRNSTDRDYYQLSITHKAKNGQTFT
                     LKNSIGYFYRSIGQSSGASSYRFTGAELSSFSELSYTKDWEANQFVAGTDLITDRFTP
                     TAPDGTLKYAHTTAGLFAQDDWTIAKKLILETGVRADQEYTLYLLPRLALLYKPLRGL
                     SARVGGGLAYKLPTVFNAQDEEEGYLQVRPIAPTVNAERSGSVNLSLNYQGRLTDEIT
                     IAIDQNFYYTRLENALIPNTDSLLKGWLVYYNAPGPVVSRGSETNARFTLDDLSLYLG
                     YSLTDARRRYLPGNPRLPLTPRSKLVAGLTWESEPHWKAGAEAFFTGSQYLDNGQKTQ
                     PFQTFDLMVQHSWGPFSILLNVENVTDTRQSRFGPLYTGTMQDPVFKEVYAPLEGRIF
                     SVAFRYSK"
BASE COUNT          657 a          750 c          968 g          673 t
ORIGIN      
        1 ccaactcccc ttcctgatgc aaaccgatct gagctttttt atgccgggga atttgatctt
       61 tacgttggat tttaccaagg atgagacggg acacatcacg cggatggtgg cattcaaacg
      121 ggaccaatgg aaaaaagtca ataaggttac gcctacgccg gctgcgctcc ggcaatacgc
      181 agggaaatac caatcgaaag atgacccgga caaccagatc agcattattg cccgggacag
      241 cgcgctggtg gtgaagcaag cgtgggacgg caaggagacc gcatatgagg cggtcgccga
      301 aaatttcttt ttcaacaacg cggcatccag tccgctcgag ttcgtccgcg atgccaacgg
      361 aggtatcaag gtggtcaggt tgttcgggaa taatgatttt attcccgttg ggcattaact
      421 attctcgtct tccattgatc ataccggaga ccagtccttt gtgttttccg agctcggcca
      481 tgatcacgcc ggcgaggtga atgaaaagga agccgtaaat gaggtattgc atgaagctgt
      541 gcacctgttt gacgggctgc cggatcgacc gaaggaaggg gatattatcg aaggcgagtc
      601 cgagtccggt caacgccata agtagcagga ccgcgtagaa ggcaagatag atactcttca
      661 cctggatgta gtgctgccgc tctgctttgg cgccggggtc gttcggtttt gcatggatga
      721 cctttttaag ctggacattc agcttctccg aacgcggctg accgagctcg atagcgaaac
      781 gggccaggac gagaccgcag atgatatagc ccagccattt gtgcagctcc cagagcttgt
      841 cgttgaaggc atgggagacg gctcgcgcct ggtcgcttga aattataacg cctttggttt
      901 ggagctggtt ttgtaccagg agcgtattct tcccggtgcg gaaaacggta gaggccagca
      961 gcacggtaat caaagatccg gtcatgacca ggaacagcgt ccagtgccag atgcggatag
     1021 cggcggaatg agggccgttg aagccggcct cgggctcttt ggggagggca cgcatggcgc
     1081 ctgtggtata actcatgtga acgggattat ggtcaaaaat agggtatacc caataataca
     1141 taaatgtttt tatttagaat accggaaggc cacgctgaag atcctacctt ccagcggcgc
     1201 atacacctcc ttaaagacgg ggtcctgcat ggttcccgta tacagcggcc caaaacggct
     1261 ttggcgcgtg tcggtgacat tttccacatt tagcaagatc gagaaggggc cccagctatg
     1321 ctggaccatg agatcgaagg tctgaaaggg ctgcgttttc tgaccattat ccaggtattg
     1381 cgaaccggtg aaaaaggctt cggcgccggc cttccagtgc ggttccgact cccaggtgag
     1441 cccggctacc aacttgctgc gcggcgtcag gggcagccgg gggttgcccg gcagatagcg
     1501 gcggcgggcg tcggtcaggc tatagcccag atagagcgaa agatcgtcga gcgtaaaccg
     1561 ggcattggtc tccgagccgc gggaaaccac ggggccgggc gcattgtaat agaccagcca
     1621 gcctttcaac aggctgtcgg tgttcgggat cagcgcattt tccaaccgtg tatagtagaa
     1681 gttctgatcg atcgcgatgg ttatttcatc ggttaggcga ccctggtagt tgagggaaag
     1741 gttgacgctg ccggagcgtt ccgcattcac ggtgggggct atcggccgca cctgcaggta
     1801 gccttcctcc tcgtcctgcg cattgaagac ggtaggcagc ttataggcca ggccaccgcc
     1861 cacccgggca ctcaggcccc gaagcggctt gtagagcagt gccagccggg gcagcaggta
     1921 gagcgtgtac tcctgatcgg cgcggacgcc ggtctcaaga atgagctttt tagcgatggt
     1981 ccaatcgtcc tgagcgaaga gaccggcggt ggtatgggcg tatttgagtg taccgtccgg
     2041 ggcggtgggc gtaaagcgat cggtgatcag gtccgttccg gcgacgaatt gatttgcttc
     2101 ccaatctttt gtatagctga gctcgctgaa gctggatagt tcggcgccag tgaaacggta
     2161 ggacgatgca ccggaggatt gcccgatgct acgataaaaa tagccgatgg aattcttgag
     2221 cgtaaacgtt tggccgttct tagccttgtg cgtaatagag agctgataat aatcccggtc
     2281 ggtgctgttc cgttggcgga acggatggag gctgtcgggc gtatgttgca cggcgtagag
     2341 gtcgccgccg gtccgacgtt cggcggacag gttgatacca aagcgaaccg tagtgttgct
     2401 gtccggatac cagaaaaagg tgggggctat agttccctgg cggatctgcg gaagatcggt
     2461 aaagccatcg ttgttgacgt cgacgggggt ttggcgactg gcggtgccca taacggtcca
     2521 gccgaaatgg cggttgcgtt tgctgtagaa gccgccgaga tcggtctcac cccgttgtgt
     2581 ttggttttcg atggcgtcga gatgaggtgt cgtatcgggc tgacgggaga tcaggttgat
     2641 gatcccggcg atggcgtcgc cgccatagag ggcggagccg gctcccttga tgacctcaac
     2701 ctgatgcagg tccagcggcg gcacttgcag gatgccgagg ctgccggaga aaccgccgta
     2761 gagcgggaag ccgtctttca gcaattgggt atagcggccg tcgagccctt gcagcctgat
     2821 cgacacatta cccgacacgg cggaagtctg ctgaagctgg gtgccggaca attcggtgag
     2881 gagctcacgg atattggcgg gcgactgggc ggtgccctct tcaatatctt cccggccgac
     2941 gacctggacg gcaatgggga tattctttaa aagctggttg gtacgggtag acaagatgat
     3001 gacgttttgt aatgtttcga tagcggtatc ggcgggagtt tttgtcgt
//
LOCUS       DATGKE010000273         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_592998, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000273 DATGKE010000000
VERSION     DATGKE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592998"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VKU83_04700"
     CDS             <1..821
                     /locus_tag="VKU83_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLI92879.1"
                     /translation="AIISDELNHASIIDGVRLCKAQRFRYKHNTMSDLETQLQAAAGA
                     RSRIIVTDGSFSMDGTIAQLDKICELADRYDAIVMIDESHSSGFLGKTGRGTHEHCGV
                     MGRIDIITGTLGKALGGASGGFTSGRKEVIEMLRQRSRPYLFSNSLAPAIAGASIAVL
                     DMLSGTTALRDKLEHNTKYFRSKMTEAGFDIRPGQHPIVPIMLYDAVVAQNFAARLLD
                     EGIYAIGFFYPVVARGQARIRVQLSAAHEQAHLDRAIAAFTKIGKELGILKKPA"
     gene            complement(822..1202)
                     /locus_tag="VKU83_04705"
     CDS             complement(822..1202)
                     /locus_tag="VKU83_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLI92880.1"
                     /translation="MEKKIINTAKAPAPIGPYSQAVQAGPLLFLSGQIAIDPSTGKVE
                     TTDIAGETHQVMRNLEAILSAAGLDFGHVVKTTILLSDMSLFTAVNEVYGQYFTGGFP
                     ARETYAVKALPKNVNIEISMVALS"
     gene            1259..1909
                     /locus_tag="VKU83_04710"
     CDS             1259..1909
                     /locus_tag="VKU83_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HLI92881.1"
                     /translation="MKVVILAEAGAPRLFRGIEPVLIRTLAELEAHRDAELYIDLDFE
                     KLWLTGRDGPAASRADALNRLLPSPVIVNAVVPTIGELGLPVIRLNGWPGFAGRDVHE
                     LSFPNADAERRLSLLYARLGQLFRPAPDWPGMISARILATVINEAWYTWEQGVSSKEE
                     IDTAMKLGTNYPLGPFEWGAQIGLENVLSLLEALARVNPRYLPAKSLKNAVGKIKI"
     gene            1974..>2191
                     /locus_tag="VKU83_04715"
     CDS             1974..>2191
                     /locus_tag="VKU83_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92882.1"
                     /translation="MGLILNIDTATATAGVCLSEAGRTLMLAESQDQKNHSSWLHPAI
                     DQLLAGTGKKARDLAAVAVTAGPGSYTGL"
BASE COUNT          476 a          595 c          668 g          452 t
ORIGIN      
        1 atgcgatcat ctccgacgag ctgaaccatg cgtccattat cgatggcgtg cggctctgca
       61 aggcgcagcg tttccggtac aagcacaata cgatgagcga cctggagacg cagctccagg
      121 cagccgcggg cgcgcggagc aggatcatcg tcaccgacgg ttccttcagc atggacggaa
      181 cgatcgcgca gctcgacaag atctgcgagc tggcggaccg ctatgatgcg atcgtcatga
      241 tcgacgaatc gcattcatcg ggttttctcg gtaagacggg ccgtggcacg cacgagcatt
      301 gcggagtcat gggccggata gacattatca cgggcaccct cggcaaggca ttgggcggcg
      361 ccagcggcgg attcaccagt ggcaggaagg aggtcatcga aatgttgaga cagcggagcc
      421 ggccttatct tttttccaat agcctggccc ctgccatcgc gggggcgagc attgccgtgc
      481 tggacatgct tagcgggacg acagctttgc gggataagct cgaacacaat acaaaatact
      541 tccggtccaa aatgaccgaa gccggcttcg atatccggcc gggtcagcat ccgatcgtgc
      601 cgatcatgct gtatgacgcg gtggtcgcgc aaaacttcgc cgcccggttg ctggacgagg
      661 gcatttatgc catcgggttt ttttatccgg tcgttgccag ggggcaggca cggatcaggg
      721 tgcagttgag cgcggcgcat gagcaggcgc acctggacag ggccattgcg gcctttacga
      781 aaatcggaaa ggagcttggc attctgaaaa agccggcgta gtcaggacag cgccaccatg
      841 ctgatctcaa tattcacatt cttagggagt gccttcacgg cataggtctc gcgggccgga
      901 aaaccgcctg taaagtactg accataaacc tcgttgaccg ccgtgaacag cgacatatcg
      961 ctcaatagga tcgttgtctt gacgacgtgg ccgaaatcaa gtccggcggc ggacaagatg
     1021 gcctccaggt tgcgcatgac ctggtgcgtt tcaccggcga tgtccgtcgt ctccactttt
     1081 cccgtggatg ggtcgatggc gatctgtccc gaaagaaaga ggagcgggcc ggcctgaacc
     1141 gcctggctgt aagggccaat gggggcggga gcttttgctg tattgatgat ctttttttcc
     1201 atgacctaaa gatcgtcaat taagtttact gcctgatcct gaattatctt tgccggcaat
     1261 gaaagtggtc attcttgcgg aagccggcgc gccgcgcctt ttccggggca tagaaccggt
     1321 gctgatccga acacttgcgg aactggaggc acaccgggat gccgaacttt atattgacct
     1381 ggacttcgaa aaattgtggt tgacgggtcg cgatggcccg gcagcttcgc gggcagacgc
     1441 gctgaaccgc cttttgcctt cacccgttat cgtgaatgct gtcgttccca cgatcggtga
     1501 gctggggctg cccgtcatca ggctgaatgg ctggcccggg ttcgcaggcc gcgatgtcca
     1561 cgagctgagc ttcccgaatg ccgatgccga aaggcgtctc agccttcttt atgcgcgtct
     1621 cgggcagctt ttcaggccgg cgcccgattg gccgggaatg atcagcgcgc ggatccttgc
     1681 caccgtcatt aacgaggctt ggtacacctg ggagcagggc gtaagcagta aggaagagat
     1741 cgatacggcg atgaaactgg gcacaaatta tcccctgggt cctttcgagt ggggcgcgca
     1801 aatcgggctc gaaaacgtcc tgtcactgct cgaagcgctg gcacgggtaa acccgcgcta
     1861 tctgcccgcg aaaagcctga aaaacgcggt tggaaaaatt aaaatatgat taaaaacgaa
     1921 ttaaaaaagc cgttgcaaaa aactgtattt tttacaatta aaattcccgc ggagtgggtc
     1981 tgatcctcaa catcgatacg gccaccgcaa cggccggcgt atgcctgtcg gaggctggca
     2041 ggaccctcat gctggcagaa agtcaggatc agaagaacca ttcatcctgg ctgcatccgg
     2101 cgatcgacca gttgctggca ggcaccggga agaaggctcg cgacctggcg gcggtggcag
     2161 tgacggccgg gcccggttct tataccggcc t
//
LOCUS       DATGKE010000274         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_775770, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000274 DATGKE010000000
VERSION     DATGKE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775770"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..564)
                     /gene="mraZ"
                     /locus_tag="VKU83_04720"
     CDS             complement(103..564)
                     /gene="mraZ"
                     /locus_tag="VKU83_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLI92883.1"
                     /translation="MIGFLGEYEATLDSKSRFLLPVGFKRQLPEEGPAQFVINRGFEK
                     CLSLYPMKSWEPIFARISQLNDFDPEVRQFRRYFLNGATMVELDSAGRLLVPQNLKEH
                     AGLDKDIVLTAAVDKIEIWDKAKYQQFFESFSPETFSSLAKQVMNPGGPLP"
     gene            complement(1231..2973)
                     /locus_tag="VKU83_04725"
     CDS             complement(1231..2973)
                     /locus_tag="VKU83_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HLI92884.1"
                     /translation="MTRFTCVWIGMAALVSCRDHPVAGTAKDIPVDRKWWKEAVVYQL
                     YPRSFKDANGDGVGDLKGIISKLDYIKSLGVNVVWLNPVYSSPNADNGYDISDYRNIM
                     RDFGTMADFDMLLYEMHRRNIRLVMDLVVNHSSDEHPWFIQSRSSRGNPYRNYYHWWN
                     AERGKPTSRWSFFDVNSDAWRFDSPTNAYYLHYFSRKQPDLNWENPAVRKEVYAIMKF
                     WLDKGIDGFRMDAFQFVAKDTTWPVFPAGYEKHINKYYGMGPHLHDYLQEMNREVLSK
                     YDMMTVAEGAGSTLEDAHDLVDSGRHELEMAYPFDGIDLGHGTRDYSLREFKQVYSKW
                     DSAFEEQGWLAIFLANHDQPRMVSHWGDDSPEFRVPSSKMLTTFIMTMRGTPYYYNGD
                     EIGMRNIRFDNIEDYRDIGTINKYQEIKNKGGDLKAFLERQKNVSRDNSRTPFQWDNT
                     ANAGFSSGTPWIKVNPDYTHINAAAEEKDPQSVLNYFRRAVALRKNNPALVYGRYTLL
                     DPDNPDVYAYTREGLGKKMLILLNFRKHPALANTGISLDGARLVLNNYPTAPAGDSLR
                     PYEARVYELGMSRE"
     gene            3017..>3282
                     /locus_tag="VKU83_04730"
     CDS             3017..>3282
                     /locus_tag="VKU83_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLI92885.1"
                     /translation="MQPKNISIADYRYDLPESSIACYPLAERDASRLLVYRAGAISET
                     VYREISAALPEGALLVFNNTKVVEARLVFEKPTGGKVEIFCLEPP"
BASE COUNT          752 a          849 c          874 g          807 t
ORIGIN      
        1 aatcgacgta gcaaccttgc ggccggatcc ggaggtagtc aagcgtctcc gccagcagga
       61 cggggaggtg ataggcgccc tcctcgctat gtagcggcgg ggtcaaggca ggggccctcc
      121 gggattcatg acctgtttcg ccaggctgct gaaagtttcc ggtgaaaagg attcaaagaa
      181 ctgctgatac ttggctttat cccaaatctc tattttgtct accgccgcgg tgaggacgat
      241 gtccttgtcg aggcccgcgt gttctttaag gttttgaggc accaggagac gcccggcaga
      301 atccagctcg accattgtcg ccccgttcag gaaatatctc ctgaattgcc gaacttcggg
      361 atcaaaatcg ttcaattggc tgatcctggc gaaaatcggc tcccagctct tcatcggata
      421 gaggctcagg catttttcaa acccccggtt gatgacaaac tgggcgggac cttcttccgg
      481 cagctgtctt ttgaagccga cggggagaag gaagcgactt ttcgagtcga gagtagcttc
      541 gtattcgccc agaaaaccaa tcatttatga agatttgaat cgtcaaaacc ttaaactaac
      601 acaaattaac actttttccc accaccataa cggaattgcg aaaatttaat ttttccacaa
      661 cagtgtattt ccccgaactg cctgttgtca ttgggtttta gggcattagc ggcttaattt
      721 ggttctattg gcccgtgcat ggatggtgaa ttacggtgga taagctgggg ataaccccca
      781 aaatcgggtg cattttatgg caactgcagg ttattccggg accccgctgc tcaaaaagct
      841 gggcctgaaa aaagaacatc gggttcggct gttacatcag ccgccggatt atttcaaatg
      901 gctggagacg gatatttccg gtcagatcgc cggcagccat gaagttccgg actgggtaca
      961 cctgtttgcc tccagccgtg ccgtgtacga aaaggagatg cgaccctcgc cggggtctgg
     1021 aaggccaacc cgaaggtcat catctgggtt tcctggtata aaaagagctc agggatcgcc
     1081 accgatctga cggaggacgg ggtgcggggc tacgcattga aaaatggcct ggtggatatt
     1141 aaggtatgcg cggtgagcgg gagctggagc ggactgaaat tagtggtgcc gttaaacaag
     1201 cgatgacgtg gcgaagattt taccgtttga tcattcccgt gacatgccaa gttcatagac
     1261 ccgggcctcg taaggccgca gggaatcacc ggcgggtgca gtcgggtaat tgttcagcac
     1321 cagcctggcg ccatcgaggc tgatgcctgt atttgccagc gccggatgtt tcctgaaatt
     1381 caacaggatc agcatttttt ttcccaatcc ctcgcgggta taggcgtaga catccgggtt
     1441 gtccgggtcc agcaaggtat accggccata gaccagcgcc gggttatttt tacggagtgc
     1501 caccgcccgg cggaaataat tcaatacgga ttgggggtct ttttcttcag cggcagcatt
     1561 gatatgtgta taatccggat tcacttttat ccagggcgtg ccactgctga atccggcatt
     1621 ggcggtatta tcccactgaa aaggggtccg gctgttatcg cgcgagacgt tcttttgacg
     1681 ttccaggaaa gccttgagat cgccgccttt attttttatc tcctgatatt tattgatcgt
     1741 gccgatatcc cggtagtcct cgatgttgtc gaaccggatg ttccgcattc cgatctcgtc
     1801 gccattataa tagtacgggg tgcctcgcat cgtcatgata aaagtcgtga gcattttgga
     1861 agaaggcacc ctgaactccg ggctgtcatc gccccaatgg ctgaccatcc gtggctggtc
     1921 atggttggcc aggaagatgg caagccatcc ctgttcttcg aatgcactgt cccatttgct
     1981 gtacacctgc ttgaattccc ggagactgta atcccgggtt ccatgtccga gatcgatccc
     2041 gtcaaaaggg taagccattt ccagttcgtg ccggccggaa tccaccagat catgcgcgtc
     2101 ctccagggta ctgcccgcac cttcggcaac agtcatcata tcgtacttgc tgagcacctc
     2161 gcggttcatt tcctgcaaat aatcatggag gtgcgggccc atgccataat atttattgat
     2221 atgcttctca tatccggcgg gaaagacagg ccaggtggta tcttttgcaa cgaattgaaa
     2281 agcgtccatg cggaacccat cgataccttt atccagccaa aatttcatga tcgcatacac
     2341 ttccttcctt acggccggat tttcccagtt cagatcgggt tgtttccggg aaaaatagtg
     2401 caggtaatag gcattggtcg ggctgtcgaa acgccacgcg tcgctattca cgtcaaagaa
     2461 gctccatcgg gaggtgggtt tgcctctttc ggcattccac caatgataat aattgcggta
     2521 tggattgccg cgggaactac gggactgaat gaaccaggga tgttcatcac tgctatggtt
     2581 gacgacgagg tccatcacga gccggatgtt ccggcgatgc atctcataaa gcagcatatc
     2641 aaaatcggcc atcgtcccaa aatcccgcat aatattgcga tagtcgctga tatcgtagcc
     2701 attgtcggca ttcggcgaag aatagaccgg gtttagccag acaacgttta caccgaggct
     2761 tttgatatag tcgagcttcg agataatccc tttcaggtcg ccgaccccat ccccgttcgc
     2821 atccttgaag ctccggggat aaagctgata cacgacggct tccttccacc atttccggtc
     2881 gacgggaata tctttggcgg ttccggcaac gggatgatcg cggcacgaga ccagcgccgc
     2941 catccctatc catacgcagg tgaatcttgt cataacagcg aataaaagta gagtttttgg
     3001 ttaatattgc ggtccgatgc aacccaaaaa tatctccata gcagactacc gctacgatct
     3061 tcccgaatca tcgatcgcct gctatccgtt ggccgagcgg gacgcgagcc ggctgctcgt
     3121 gtaccgggcc ggagcgatca gcgaaacggt ttaccgcgag atttccgcag cgttgccgga
     3181 aggcgccttg ctggttttta ataacacgaa agtcgtcgaa gcacgcctcg tctttgagaa
     3241 gccgacgggc ggcaaagtag agatcttttg cctggagccg cc
//
LOCUS       DATGKE010000275         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_196870, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000275 DATGKE010000000
VERSION     DATGKE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196870"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VKU83_04735"
     CDS             <1..233
                     /locus_tag="VKU83_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92886.1"
                     /translation="YGYDKALANYKQHYDSPDKMGKLFFTLLSIKELSPEYYFVIGKW
                     RLKRKAEDVGGTYTLLFRKIGGRWQIVVDHTS"
     gene            complement(244..975)
                     /locus_tag="VKU83_04740"
     CDS             complement(244..975)
                     /locus_tag="VKU83_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HLI92887.1"
                     /translation="MYSLTGAVLIMLGLAGYDWWAERNAGFVRYAAFGIDIPVNYSIH
                     GIDVSKYQNIIDWSSVKDMKVGAVQLSFAFIKATEGLGNEDVYFQRNWKKAKDAGIAR
                     GAYHFFLSTKSGKAQAENFINSVELQPGDLPPVLDIEQTYGVSPEKLRERAKEWLQTV
                     QGYYHVQPIVYTNVDFYKQFLKDDFDAYPLWVAHYLQKERPSIYRAWAFWQHSESGRV
                     NGILTKVDFDVFNGDSTEFRRLLIN"
     gene            1094..2122
                     /locus_tag="VKU83_04745"
     CDS             1094..2122
                     /locus_tag="VKU83_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HLI92888.1"
                     /translation="MPEFNWNDTTNLLSKLTLRRLVNGIKVLGSYYVSKWTKKAIQWG
                     YPVSLSFEPTTSCNLRCPECPSGLRAFTRPTGMLQDDFFRQTIDQLSGDLLYLIFYFQ
                     GEPYLNPSFLDMVAYASAKGIYTATSTNAHYLNDRNARRTVESGLDRLIISIDGTTQD
                     VYEQYRIGGKLEKVLEGTRNIVRWKKELKSKKPFVFFQFLVVRPNEHQLDDIRRLARE
                     TGVDEVRFKTAQVYDYRNDPNGLIPAGERYSRYRKDKNGEYVFKNTLENHCWRLWHAT
                     VITWDGLVVPCCFDKDAQHPLGDLKDKPFREVWRDPKYVEFRRQILKSRKNIDICANC
                     SEGTKVWD"
     gene            2158..2637
                     /locus_tag="VKU83_04750"
     CDS             2158..2637
                     /locus_tag="VKU83_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLI92889.1"
                     /translation="MPLSASILTRLQHQHETIGELIGGLDEQALRRNVNPGKWSALDN
                     IAHLACYQPVFLHRLERIRAENSPAFDRYVADSDPAFPAYQQSPPGELLAGLREQRSV
                     ICSKLQGMDETGLRRTGLHPRFGLLDVRQWSEFFLLHEAHHLYTLFSLVQEVRKPAR"
     gene            2669..>3228
                     /locus_tag="VKU83_04755"
     CDS             2669..>3228
                     /locus_tag="VKU83_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLI92890.1"
                     /translation="MALENEINQRKFRTEYQKARINIIYTYNWLSERINSIFKDWDIT
                     PQQFNILRILRGEGKPLSTLQIRQRMLDKMSDTSRIVDRLLKKGLVRKTPNDEDRRLV
                     DVVITPKGRKLLEKIDPFEENADKMMMSLTEEETKTLNHLLDMLRNTATIFLFLLGSV
                     LFAKAQPKYEFRAAWVASVENIDWPS"
BASE COUNT          766 a          888 c          844 g          730 t
ORIGIN      
        1 cctatggata tgacaaggcg ttggccaact acaaacagca ttatgacagt cccgacaaga
       61 tggggaagct tttttttacc ctgctcagca ttaaggagct gtcgcccgaa tattatttcg
      121 ttatcggcaa atggcggctt aaacgtaagg cggaagatgt cggcggcacc tacacgctgc
      181 ttttccgcaa gatcggcggc cgttggcaga tcgtagtgga tcatacgagc taagactgcg
      241 cgttcaattg atcaggagcc ggcggaattc ggtgctgtcg ccattgaata catcgaaatc
      301 cactttggtt aggatgccat tgacgcggcc cgactcgctg tgttgccaga acgcccaggc
      361 ccggtagatg ctgggtcttt ctttttgcag ataatgcgct acccagagcg ggtaggcgtc
      421 gaaatcgtcc ttgaggaatt gtttgtagaa atctacattg gtatagacga tcggctggac
      481 atgataatag ccctgtaccg tttgcaacca ctctttggct cgttcccgta gcttttcggg
      541 ggatacccca taggtctgct cgatatccag cacgggaggc aggtctccgg gctgcaattc
      601 caccgaattg atgaaattct ccgcctgagc cttgccgctc ttggtggaca gaaagaaatg
      661 ataggcgccc cgcgcgatac cggcatcctt cgcttttttc caattgcgct ggaaatagac
      721 atcttcattt cccagccctt cggtcgcctt gatgaaagcg aagctcagct gtacggcacc
      781 taccttcatg tccttcacgg agctccaatc gatgatattc tggtatttgg atacgtcgat
      841 gccgtgtatg ctgtaattta cggggatgtc gataccgaat gccgcatagc ggacaaaacc
      901 ggcgtttctt tccgcccacc agtcgtagcc ggccaaaccc agcatgatca acacagcgcc
      961 ggtcagagag tacactatta tcttccaaag tctgtttttc ctcgatctat tcgccataat
     1021 gagttcgggc acggctggac ggggtcgggg tccagcgggc agatacaaac ataattagta
     1081 tttttatact tccatgcccg aatttaactg gaacgataca accaatctcc tgtccaagct
     1141 taccctgcgg cgactcgtca acgggatcaa ggtactcggc agctattacg tgagcaaatg
     1201 gaccaaaaaa gctatccaat ggggatatcc ggtctccttg tcgttcgaac ctaccacctc
     1261 ctgtaacctg cgttgtcccg agtgccccag cggcctccgg gcattcacgc ggcccacagg
     1321 catgctgcaa gacgattttt tccggcagac catcgaccag ctatccgggg atctgcttta
     1381 tctgatcttc tatttccagg gagagcccta tctgaacccc tctttcctgg acatggtggc
     1441 ctatgcttcc gcgaagggga tctataccgc cacctcgacc aacgcgcatt atctgaatga
     1501 ccgcaatgcc cggaggaccg tcgaaagcgg gctcgaccgg ctcatcattt ccatcgacgg
     1561 cactacccag gatgtctacg aacaataccg gataggcggc aagttggaaa aagttctgga
     1621 gggtacacgc aacatcgtcc ggtggaagaa agagctgaaa agtaaaaagc ccttcgtgtt
     1681 ctttcagttc ctggtggtgc ggccgaacga acatcagctg gacgatatcc ggcggctcgc
     1741 ccgggagacc ggcgtcgacg aggtccggtt caagacggcg caggtctatg actatcgcaa
     1801 tgaccccaac gggctgatcc ccgccggcga aagatatagc cgctaccgga aagataagaa
     1861 cggtgaatat gtgtttaaga atactttgga gaaccattgc tggcgtcttt ggcacgctac
     1921 ggtgatcacg tgggatgggc tcgtcgtgcc ctgctgtttc gataaggatg cgcagcaccc
     1981 cttgggcgat ctcaaggata agcccttccg ggaggtctgg cgcgatccca aatacgtcga
     2041 gttccggcgg cagatcctga aaagccgcaa gaatatcgat atctgcgcca attgcagcga
     2101 aggtaccaag gtctgggact agatgggtcg aacaaacgtt aacgccaaat tgcatatatg
     2161 ccgctatcag catccatcct cacccggctt cagcatcagc acgagaccat cggcgagctt
     2221 atcggcggtc tcgacgaaca ggcgctccgc cggaatgtca accccggcaa gtggagcgcg
     2281 ttggacaata ttgctcatct agcctgctat caacctgtgt tcctgcaccg gctcgaacgg
     2341 atacgagccg aaaattctcc ggccttcgat cgctatgtag cggattcgga tcctgccttc
     2401 cccgcttatc agcaaagccc gcccggtgag ctccttgccg ggcttcggga acagcgctcg
     2461 gtcatttgtt ccaaactgca agggatggac gaaacgggtt tgcgccggac ggggctccat
     2521 ccccgctttg ggctgctgga cgtgcggcaa tggagcgagt tctttctcct gcatgaagcc
     2581 catcaccttt acaccctatt ttccctcgta caggaggtgc gcaagccggc gcgatgaata
     2641 tttctgtact tttatggata atccagccat ggcacttgag aacgaaataa atcagcgaaa
     2701 attccgcacc gaatatcaga aagcgaggat caatatcatc tacacgtata actggctctc
     2761 cgagcggatc aactccatct tcaaagactg ggatatcacc ccgcagcagt tcaacatcct
     2821 ccgcatcctg cgtggagaag gcaagcccct ttccacgctg cagatccgcc agcgcatgct
     2881 ggacaagatg agcgatacca gccgtatcgt tgaccgtctc ctcaaaaaag gcctggtaag
     2941 gaaaacaccc aatgacgaag atcgccggct ggtcgatgtg gtgatcacgc cgaagggccg
     3001 gaagcttttg gaaaagatcg atcccttcga agaaaatgcg gacaagatga tgatgagcct
     3061 aacggaagaa gaaacgaaga cgctgaacca cttgctcgac atgctgcgca atacggcgac
     3121 aatattcctg tttttgctgg gttccgttct gttcgcgaag gcgcagccga aatatgagtt
     3181 ccgggccgcc tgggtagctt ccgtggaaaa tatcgactgg ccttccaa
//
LOCUS       DATGKE010000276         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_334196, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000276 DATGKE010000000
VERSION     DATGKE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334196"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VKU83_04760"
     CDS             complement(<1..206)
                     /locus_tag="VKU83_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92891.1"
                     /translation="MDLSEEYHKWHSPVINREFEMLVFGYAGTPLILFPTSKGTYYQN
                     KDHGLVGTARWFLENGRVKIYCPD"
     gene            321..1124
                     /locus_tag="VKU83_04765"
     CDS             321..1124
                     /locus_tag="VKU83_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLI92892.1"
                     /translation="MMTTSILQENFTVDSAFLKRPVRVDLYLSGGVTVGSETGLLLIN
                     DGQDLTRMGFAGMLEQLDSHEGLAPLLCVGIHAGPERKMEYGVASRVNDQGWGKSAGA
                     YSRFVLEELIPVIGKRFAVSRFRMKAMAGFSLGALSAMDIVWKHPREFSIAGLFSGSF
                     WWRTKDKLDPSFDERRDRIMQDEVRKGAYHRGLRFFFECGTEDELDDRNGNGVIDSID
                     DTKDLIAELVRKGYDPVRDIRYLEIEGGRHDVATWARAMPEFLLWGWRK"
     gene            complement(1223..1720)
                     /locus_tag="VKU83_04770"
     CDS             complement(1223..1720)
                     /locus_tag="VKU83_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92893.1"
                     /translation="MDKKIFIALVVVVTAATTGCAKHHFLPIVTPPVSRIFTVSSLNH
                     TADTVNVGDTVYLDAAGTIYDTSKTIYVSMTASYTANGASTVYNYGSAGSPIKVKYVT
                     GADSAGVFDWTATIPLIGATSVPAKTKLTIAATFQYQLSLSSELPSSLSISDAGQKTK
                     TVYVN"
     gene            complement(1737..>2036)
                     /locus_tag="VKU83_04775"
     CDS             complement(1737..>2036)
                     /locus_tag="VKU83_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92894.1"
                     /translation="EDIFAFTKTKSDEDRHMLSVTMDMLNVGNFLDRNWGIVKTPVNS
                     SFLRFEGMAADGKTPLFSFPYQDATNLVPQQASFANSTGTSSRWAMQIGVRYTFN"
BASE COUNT          443 a          463 c          644 g          486 t
ORIGIN      
        1 ctgtccgggc aatagatctt tactctgccg ttctcgagga accagcgggc ggtgccgacc
       61 agtccgtggt ctttattctg gtaataagtg cctttagacg ttggaaagag gatgagcgga
      121 gtaccggcgt agccaaaaac cagcatttcg aactcgcggt tgatgaccgg ggagtgccat
      181 ttatgatatt cttcgctcag atccatgtgc aggcacagtt ttgggaggtg tagccaaacc
      241 tacagaaaaa aaaccacccg acaatggcag gctaaaatgt cgcagaatat ttccctattt
      301 ttggcgttat cttgactcat atgatgacta cgagcatttt acaggaaaat ttcacggtag
      361 attcggcatt cctgaaacgc ccggtaaggg tggatctgta tttgtcagga ggagttacgg
      421 taggctctga gacgggcctt ctattgatca acgatggcca ggacctcacc cgaatggggt
      481 ttgcgggcat gctggagcag ttagacagcc acgaggggct ggcgccttta ttgtgcgtcg
      541 gcatccatgc cgggccagag cgcaagatgg aatatggggt ggcttcgcga gtgaatgatc
      601 aaggctgggg caaatcggca ggggcctata gccgctttgt tctggaggag ctaatccccg
      661 tcatagggaa gcggtttgct gtgtcgcgct tcaggatgaa ggcgatggcg ggtttttcgc
      721 tcggtgcgct tagcgccatg gatatcgtat ggaagcatcc ccgggagttc agtattgccg
      781 ggcttttttc aggcagcttt tggtggcgga cgaaagacaa gctcgaccct tcttttgatg
      841 aaaggcggga caggattatg caggacgagg tgcggaaggg agcctaccac cgtggtttga
      901 ggttcttctt tgaatgcgga acggaagatg aattggacga ccggaacggc aatggggtga
      961 tcgattcgat cgacgatacg aaggacctga tcgcggagct ggtcaggaaa ggctatgatc
     1021 cggtcaggga tatccgctat ctggagatcg aaggcgggcg tcatgacgtg gctacctggg
     1081 cacgggcgat gcccgagttt ctgttgtggg gttggcgaaa atagcggtgc aggctgccaa
     1141 agacggagcg gactgacgga agacaaataa aaagggtgcc tcgtcggggc accctttcgt
     1201 gattttgtcc tgtttccgaa acttagttaa cataaacggt cttggttttc tggccggcat
     1261 cgctgatgct gagactggac ggcagctcgc tgcttaagct gagctggtac tggaatgtag
     1321 cagcgatggt cagcttggtc ttggcgggca cggaggtggc gccgatcagg gggatcgtcg
     1381 cggtccaatc gaagacgccg gcgctgtctg cgccggtaac gtacttgact ttgatcgggg
     1441 agccggcgct gccgtaattg tagacggtcg atgctccgtt cgcggtataa ctggcggtca
     1501 tgctcacgta gatggtcttg ctcgtatcat aaatggtccc ggcggcgtcc aggtacacgg
     1561 tgtcgccaac gttgacggtg tcggcggtgt ggttgaggga gctgacggta aagatcctgg
     1621 ataccggggg cgtaacgatc ggaagaaagt gatgttttgc acatccggtt gtggctgcag
     1681 tgaccacgac gaccagggct ataaagattt tcttgtccat aattattttt ttcagattag
     1741 ttaaacgtat accgaacgcc gatttgcatg gcccaacgcg aactggttcc ggtgctgttg
     1801 gcgaagctgg cctgctgggg gaccaggttg gtagcatcct ggtagggaaa ggagaacaac
     1861 ggcgtcttgc cgtcggccgc catcccttca aaacgcagga agcttgagtt gaccggcgtc
     1921 ttgacgatgc cccagttccg gtcgaggaag ttaccgacgt tcaacatatc catggttaca
     1981 ctcagcatat gcctgtcctc atccgacttg gttttcgtaa aggcgaaaat gtcctc
//
LOCUS       DATGKE010000277         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_329083, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000277 DATGKE010000000
VERSION     DATGKE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329083"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VKU83_04780"
     CDS             complement(<1..249)
                     /locus_tag="VKU83_04780"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLI92895.1"
                     /translation="MPRILRVGLTGGIGSGKSTVAQIFEVLGIPVSYADREAKRVMDQ
                     DPALRAAIVARFGPEAYSGETLDRKWMAARVFSDPSRLE"
     gene            complement(301..648)
                     /gene="yajC"
                     /locus_tag="VKU83_04785"
     CDS             complement(301..648)
                     /gene="yajC"
                     /locus_tag="VKU83_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLI92896.1"
                     /translation="MTHSFLVVLLQAAPGGGGSFQLLLIGGMFLVFWLFMIRPQQKKM
                     KLAKKFQEEMQKGDKIVTNAGIHGTVSKLNDDGTTIMMETSPGSYLKIEKQAISIEWS
                     QNINKAANVPEKK"
     gene            complement(675..1166)
                     /locus_tag="VKU83_04790"
     CDS             complement(675..1166)
                     /locus_tag="VKU83_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HLI92897.1"
                     /translation="MLIPCPAPRLRPSPSRASFLVIPVLAAIAILSLPGCGVGDNRAI
                     AMQQVMRDSARYTTIQWLDSTRDFGTIPEGQTLDVSFRFRNTGASPLVITNVRPSCGC
                     TVADPSGQKPVAPGGEGKISATFNSEGHPGVNRKTLFVTANTKGSQNYSLHFTVVVNK
                     KTS"
     gene            complement(1228..2154)
                     /gene="nusB"
                     /locus_tag="VKU83_04795"
     CDS             complement(1228..2154)
                     /gene="nusB"
                     /locus_tag="VKU83_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLI92898.1"
                     /translation="MISRRNIRVKVMQTLYTLEAQEGTAKPGEPARILQKHFDQSRQL
                     LTYLIYCLTEVARYAETDSRLRASKHLPTEKDLHVNVKLAGNEQLWKILEDPSWKSAV
                     AADKPQLQDNKELVRRLYGDLTATEEYAKYIAAPGRERRTEKEMIEFIFSGVILPSES
                     FLAHIEELFSNWEDDGEMICQLLMGYIAKPGSLNFQEIISKEKQEFALSLLTTVLEKK
                     EVVLELIRPRLKNWDAERIATLDMILMQMGVAEFLYFETIPPKVTINEYIDLAKDYST
                     PQSGQFVNGILDNIHKDLVREDKMHKVNFKAS"
     gene            2348..2758
                     /locus_tag="VKU83_04800"
     CDS             2348..2758
                     /locus_tag="VKU83_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92899.1"
                     /translation="MRRLMVITGIFAAAVIALAIHIYVVTHPHFGGRSRGMARIDLHQ
                     PINQTDAERITAWLYRHEGVDHVLVSTKSAITVFTYSPLVADAGRIASAFRDSLSYPR
                     AVRYLPSEVEIKRSCPLRATPVTNGLYEFLKRIF"
     gene            2775..3341
                     /locus_tag="VKU83_04805"
     CDS             2775..3341
                     /locus_tag="VKU83_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92900.1"
                     /translation="MKKTKSRATQAILGGAVLLLAVTLSCHKSGRPAGNQTFYDSLGG
                     AVLVPDPANFGQSVEKGYLTIRTIVDCALFLIQVDPVINGYFSIMVAEDTAHTTPTEY
                     DKLSLGITNFLAVAAGSKDYSYTGPTLLDAHNPSTNPNVPGPIDSGAFNEFAFDLGRA
                     AAQSGLSNQLISQLGDLLYRYEGQVVQP"
BASE COUNT          796 a          973 c         1085 g          903 t
ORIGIN      
        1 ttctagccgt gaggggtcgg aaaaaacgcg ggcagccatc cacttccggt caagggtctc
       61 ccccgaataa gcttcggggc caaaacgggc gacgatcgcc gcgcgcaggg ctgggtcctg
      121 gtccatgacc cgctttgcct cccggtcagc gtaggacaca ggaattccca gcacttcaaa
      181 gatctgtgct accgtgcttt taccggaacc gatcccgccg gtcaggccga cccgtaatat
      241 ccgcggcatg aggctatgca taaagaaccc ggcgctcggc cgggtcctgg gaatgaatgc
      301 ttattttttc tcgggaacgt tggccgcctt gttgatgttc tggctccact caatggaaat
      361 ggcctgtttt tctattttga gatagctccc cggactggtt tccatcatga tcgtcgtgcc
      421 atcatcgttc aacttgctga cggtcccatg gatgcctgca tttgtcacga tcttatcgcc
      481 tttctgcatt tcctcctgga atttcttggc caatttcatt tttttctgct gcggccggat
      541 catgaaaagc caaaaaacca ggaacatgcc gccgatgaga aggagctgaa aagatcctcc
      601 tccgccggga gccgcctgca aaaggactac taaaaaagaa tgcgtcattg ttttcttgtt
      661 tgtattgagt tgttttatga tgtcttctta ttgactacta cagtaaagtg cagggaatag
      721 ttctggctgc ccttcgtgtt ggcggtaacg aataaggtct tgcggttgac gcctgggtgc
      781 ccctcgctgt taaaggtggc gctgatcttg ccttcgccgc cgggggcaac tggtttttga
      841 ccggaagggt cggcgaccgt gcagccgcag ctgggcctga cattggtgat aacaagcggt
      901 gaggcgcccg tgttccggaa ccggaaggag acgtccagtg tctggccttc ggggatggtg
      961 ccgaagtccc gcgtggaatc caaccattga atggtagtgt accgggcgga gtcccgcatc
     1021 acctgctgca ttgcaatcgc ccggttatca cccacgccac agccgggcag cgacaatatg
     1081 gcgatggcgg ccagcaccgg gataaccagg aaggaggccc gggaagggct gggacgaagc
     1141 cgcggggcgg ggcatggaat cagcatggat gaggaatttt tttttgcaaa cgtacaggtt
     1201 ttttggttcc ggcagcgtaa aaccggatca gctggctttg aaattgacct tgtgcatttt
     1261 gtcctcacgc acaaggtcct tgtgaatgtt gtccaggatt ccgttcacga actggccgct
     1321 ttgaggggta ctgtaatcct ttgccaggtc gatgtattca ttgatggtta ccttcggcgg
     1381 aatggtctcg aaatagagga attccgccac gcccatctgc atcaggatca tgtcgagagt
     1441 ggcgatccgt tccgcatccc agttctttag ccgcgggcgg atcagttcca ggacgacctc
     1501 ctttttttcc agtacagtcg tgagaaggct aagggcaaat tcctgctttt ccttgctgat
     1561 gatctcctgg aaattgaggg agccgggttt ggcaatatat cccatcagca gctggcagat
     1621 catttcgccg tcatcctccc agttcgaaaa caattcttcg atatgcgcca ggaacgactc
     1681 gcttggcagg atgacaccgc tgaaaatgaa ttcgatcatt tctttttcag tccggcgctc
     1741 ccggccgggg gcggcgatat attttgcgta ttcttcggtg gcggtgaggt cgccgtagag
     1801 ccgccggacc agttccttgt tatcctgcag ttgcggtttg tccgcggcga cggccgattt
     1861 ccaggagggg tcttccagga tcttccacag ctgttcgttg cccgccaact tgacatttac
     1921 gtgcaggtct ttctccgtcg gaagatgttt ggacgcgcgg agcctcgaat ccgtttctgc
     1981 gtaccgggct acctccgtaa ggcaatatat aaggtaagtt aataactgtc tggactgatc
     2041 gaaatgtttc tgcaagatcc tggcaggctc tcccggtttc gcggttcctt cctgcgcttc
     2101 cagggtgtag agggtctgca ttactttcac cctgatattt cttctgctga tcattataag
     2161 aacttggttt gcgggggcaa atatagacaa tttttaccgg cggcgatgcg cgcagcaggt
     2221 tggtgcgtgg gacacgggat tgcgcgacgg aggtgcggcg ggcgaagaat gttaaatcgt
     2281 tgcagtcttt ccgtcaggcc gcctgaattt tttgctggac cgggtggagt cgggtaattt
     2341 gcgccgcatg agacggctga tggtgataac gggaatattc gcggcagcgg tgatcgcgct
     2401 tgcgatccat atttacgtcg ttacccatcc gcactttggc ggccgctcgc ggggcatggc
     2461 gaggattgat cttcatcagc ccatcaacca gaccgatgca gagcggatca cggcctggct
     2521 ctaccggcac gagggggtgg accatgtgct ggtcagcacg aagtcggcga ttacggtttt
     2581 cacttattcg ccgctggttg ccgatgccgg acggatcgca agcgccttcc gggacagcct
     2641 gtcctatccg cgggcggtgc ggtacctgcc gtcagaggtt gaaataaagc gcagttgtcc
     2701 gttgagggcg acgccggtga ccaacggcct gtacgaattt ctaaaacgaa tcttttgagc
     2761 cgatatcaat aatgatgaag aaaacgaaga gccgggcgac ccaggcgata ctgggaggag
     2821 ccgtactatt actggcggta acgctgtcct gtcataagag tggcagaccg gccgggaatc
     2881 aaacgttcta tgattcgctc ggcggcgccg tgctggtacc ggatccggca aatttcggcc
     2941 agtcggtcga gaagggatac cttacgatcc ggacgatcgt cgactgcgcg ctgttcctga
     3001 tccaggtcga cccggtgatc aatggctact ttagtattat ggtggccgag gatacggctc
     3061 atacgacgcc gaccgaatac gacaaactca gcctgggcat cacgaatttc ctggctgtgg
     3121 cggcgggttc aaaggattac agctataccg gcccgacgct tttggatgca cataacccct
     3181 ccaccaatcc caatgtccct ggcccgatcg acagtggcgc gttcaacgaa tttgcttttg
     3241 acctcggccg ggccgcggcg cagtccggat tgtctaacca gctgatcagc cagctggggg
     3301 accttcttta tcgatatgag ggtcaggtgg ttcaaccata accctctggt taccatcaca
     3361 tgaaggccga ctgacaggta tgtcgcggcc ggctgaaaaa ttgacacgag acgggcagac
     3421 tggccggctg tccggtctaa caaactgtaa atatggctta tgcggccggt ttggtataaa
     3481 attcgtgtag ctttgccaac ctgcgtcctg tcaggttcaa gtctttcaac aaaacaaaaa
     3541 tcgatacaac tatgggcaaa aagttaaacg ttaccccgtt agcagatcgg gtaattgtga
     3601 agccagctcc agctgaagaa aaaactgccg gcggcatcat cattccggat acggcaaagg
     3661 aaaagccgca gcgcggaacg gtcgtagcgg ccggaccggg aaaaaaggat gagccggtta
     3721 cggtgaagat tggcgatacc gtgctgtatg gtaaata
//
LOCUS       DATGKE010000278         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1119113, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000278 DATGKE010000000
VERSION     DATGKE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119113"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..471
                     /gene="rpsQ"
                     /locus_tag="VKU83_04810"
     CDS             214..471
                     /gene="rpsQ"
                     /locus_tag="VKU83_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLI92901.1"
                     /translation="MVERNLRKTRIGVVKSNKMNKTITVTVERKVKHPIYGKFVKKTT
                     SFHAHDEKNECGVGDVVKIMESRPISKSKRWRLVEVVEKAK"
     gene            607..975
                     /gene="rplN"
                     /locus_tag="VKU83_04815"
     CDS             607..975
                     /gene="rplN"
                     /locus_tag="VKU83_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLI92902.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQDYAGIGDKIVVTVK
                     DALPSGGIKKGTVAKAVIVRTKKKLRRKDGSYISFDDNAVVLLNASDEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            1051..1389
                     /gene="rplX"
                     /locus_tag="VKU83_04820"
     CDS             1051..1389
                     /gene="rplX"
                     /locus_tag="VKU83_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLI92903.1"
                     /translation="MSNRFKPKFNIKKGDSVVVITGDDKDVKKARKVLEVYPEEAKVL
                     VEGVNIVTKHTKPSAQNTKGGIIRKEAPIHISNVQLWDAKGGGPSKVSRSREDGKLVR
                     IAKKSGEVIK"
     gene            1386..1955
                     /gene="rplE"
                     /locus_tag="VKU83_04825"
     CDS             1386..1955
                     /gene="rplE"
                     /locus_tag="VKU83_04825"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLI92904.1"
                     /translation="MSTAKYTPRLADKYKKEVVPALMKKFGYSTVMQAPRLEKICINR
                     GINGAVTDKKLVEVAVDELTMITGQKAVTTQSKKDISNFKLRKNMPIGARVTLRGERM
                     FEFLDRLVSTALPRVRDFKGINEKSFDGRGNYTLGITEQIIFPEIDIDKVNKITGMDI
                     TFVTTAGTNEEAFELLKELGMPFRNIRKD"
     gene            1972..2241
                     /gene="rpsN"
                     /locus_tag="VKU83_04830"
     CDS             1972..2241
                     /gene="rpsN"
                     /locus_tag="VKU83_04830"
                     /inference="COORDINATES: protein motif:HMM:NF006477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HLI92905.1"
                     /translation="MAKESVKARQRKREVLTAKYAEKRAALKAAGDYKALDLLPRNAS
                     PVRLHNRCQLTGRPRGYIRYFGISRVIFRDMALQGKIPGVRKASW"
BASE COUNT          751 a          553 c          765 g          469 t
ORIGIN      
        1 atctgaaagc aaagatccag gaagatgagt tgcggctgaa gaagctggag ttttctcatg
       61 cgatttctcc gctggaaaac ccgatgagca tccgtagcct gcgaaaggat attgcgcggt
      121 tgaggacggc gctccggcag cgggtcgggc aaaagaaaga ggccgccaat caataaaatc
      181 gagtacgcag tctgaacatt aaatgtcaaa acaatggttg aaagaaattt gcgcaaaacc
      241 aggatcggag tggtcaagag caacaagatg aataagacca tcaccgtgac ggtcgaaagg
      301 aaagtgaagc acccgatcta tgggaagttt gtgaaaaaga cgaccagttt ccacgctcat
      361 gacgaaaaga acgagtgcgg ggtcggcgat gtagtaaaga tcatggaaag ccggcccata
      421 agcaagtcca agcgctggcg ccttgtcgag gttgtagaga aggctaagta ggcatcaaaa
      481 taacggcgga ccacgggtca ggaggaacaa gggcccgcaa ttccgcgaag ttcccatcat
      541 gagggtgcga gggtgatggg aatatttcgg agaaaacata acccgagcac taaaaagcat
      601 acaaaaatga ttcagcaaga aagcaggtta aatgtagctg ataacagcgg cgccaaagaa
      661 gtgttgtgca tccgcgtatt gggaaacagc ggacaggatt atgcgggcat cggggataag
      721 atcgttgtca cggtcaagga cgcgctgcct tccggcggga tcaagaaggg taccgttgcc
      781 aaagcggtga tcgtccggac caagaagaaa ttgcgtcgca aggatggaag ttatatcagc
      841 tttgacgaca acgcggtggt attgctgaat gcatccgatg agccgcgggg tacccggatc
      901 ttcgggccgg tcgccaggga gttgcgcgac aaaggataca tgaagatcat ttcgttggcg
      961 cccgaggtac tgtagcggag ccgttggcga ggggcggcta cttactgaag gtcagacata
     1021 agcataactc gatgttataa acatacaaca atgagcaaca gatttaaacc gaagtttaat
     1081 atcaagaaag gtgacagcgt ggtcgtgatc acgggagacg acaaggatgt gaaaaaggcc
     1141 cggaaagtcc tggaagtcta tcccgaagag gcgaaagtgc tcgtcgaggg agtcaatatc
     1201 gtgaccaagc atacgaagcc ttcggcgcaa aataccaaag gcggcatcat aaggaaagag
     1261 gctccgatcc atatctccaa tgtccagctg tgggatgcca agggcggcgg cccttcgaag
     1321 gtctcgcgct cccgggagga cggaaaactt gttcggatcg ctaaaaaatc aggggaggtt
     1381 atcaaatgag tactgcaaaa tacactccgc gtctggcaga taagtacaaa aaggaagttg
     1441 tacctgccct catgaagaag tttgggtaca gcaccgtgat gcaggcgccc aggctggaga
     1501 agatctgcat caaccgcggc ataaatggcg cggtcaccga caagaaactg gtggaagtcg
     1561 cggtcgacga actgacgatg atcaccggcc agaaggcggt gacgacccag tcgaagaagg
     1621 atatttccaa tttcaagctg cgtaaaaata tgccgatcgg cgccagggtg acgttgcgcg
     1681 gcgagaggat gttcgagttc ctggaccggc tggtttcgac ggcgttgccg cgggtaaggg
     1741 atttcaaggg gattaatgaa aagtcgttcg acggccgcgg taattatacg ctcggcatca
     1801 cggagcagat tattttcccc gagatcgata tcgacaaagt gaacaagata accgggatgg
     1861 atatcacgtt tgtgacgacg gccgggacga atgaggaagc ttttgagttg ctgaaagagc
     1921 tgggaatgcc gttccgcaat attcgcaaag actaaataaa actttataaa catggctaaa
     1981 gagtctgtaa aggctagaca aaggaaacgg gaagtgctga cggcaaaata tgccgagaag
     2041 cgggcagccc tgaaggccgc cggcgactac aaggcgctgg atctattgcc gcggaacgcg
     2101 tctccggtgc gcttgcacaa ccgttgccag ctgacgggac gtccgagggg ttatatccgg
     2161 tactttggga tttcccgggt gatcttccgt gatatggcgc tgcagggcaa gatcccgggc
     2221 gtgaggaagg ctagctggta aggaagtcca gccgggcaag acgccggaaa ggggacagat
     2281 cccgaagaga gaaagatgga tatcgcattg taaataaaat tgaaaacggc ggaccctagt
     2341 ccgcgcaaac agaacatcaa caatggttac tgatccaata gcagattttc tgacgagaat
     2401 ccgcaacgcg cagatggcga atcaccggat agtggaaatc ccggcttcca acctgaagaa
     2461 gcggatgacg gagatcctct atgacaaggg agatatcctg aagtacaagt tcgaggatga
     2521 caacaagcag ggcgtcat
//
LOCUS       DATGKE010000279         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_799406, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000279 DATGKE010000000
VERSION     DATGKE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799406"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2145)
                     /locus_tag="VKU83_04835"
     CDS             complement(<1..2145)
                     /locus_tag="VKU83_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92906.1"
                     /translation="MLFVRPVTLDTTTMLLNFFKTAWRSMRRNKLTTMINIAGLALGI
                     TACLIIFLLVRFEFGYDRFHPEGDRIYRVVATLGGMGGERDFGFVTMGLPNDARREIS
                     GLQTVSAFDDLTSSVIVPRPGQTNKVFDAAKRGEQTSPVIIAQPEYFQIFQYQWLAGN
                     AATALNEPFKVVLSASEAEKYFGPAQPEEWLGRPLIYRDSLRVTVSGIVADWKQKSDF
                     GFKDFISYATIGHSFLKYDYDPTNWGMWDYDAQAIVKLAPGVSPAQIEKQFPAFVARH
                     RVGPKDSKVKISLQPLQDIHFNEKYSDAFSRKTSLPTLYGLAAIAIFILVIAAINFIN
                     LATAQSVRRAREIGVRKVLGGRRGALILQFLSETLVLVIAAATLALLITDPLLAAFGS
                     LLPRGVHLNVFQWPAALFLAATVVVTSFLAGFFPARVLSSYRPAISLKGQGVQHLNGK
                     SYLRKALIVFQFTVSLVFIIGTMIISRQIHYVLTSDLGFDHDAIIVIRAQEDGPQHHR
                     AALANLIREIPGVKMVTRHMEMPTAAGHPGTIIQLSSDANRKTMASFDMVDTSYLGLF
                     GMHLVAGRNLFPADTIREFLVNETCAKQLGFQHPANALGQIVNIGMNNGAGPIVGVVR
                     DFHSQSLHEPITPFFISTFERAQRDVSVKLATQGQTADQLTAILGKLAKAWREVYPDR
                     KFEYLFFDESIAKLYDTERKTAAIMDIAMGIAI"
     gene            2286..>2709
                     /locus_tag="VKU83_04840"
     CDS             2286..>2709
                     /locus_tag="VKU83_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI92907.1"
                     /translation="MPRHLRHLILLYPLLLVALLSYPQQRHRDTLILHFAFNSHRIPS
                     QDSTRLSQALASEKIDSVQIDGYTDKVGSAAYNLALSRRRAKAASRLISRETVDDSRL
                     HAAGHGKAPTTQWSDSANRRVEVVLFYTRPPEASGTPPA"
BASE COUNT          602 a          797 c          712 g          598 t
ORIGIN      
        1 gatggcgatg cccatggcga tgtccataat cgcggctgtc tttctttcgg tgtcgtatag
       61 tttggcgatg gattcgtcaa aaaataggta ctcaaacttt cgatccggat atacctcccg
      121 ccaggccttc gccagctttc ccagtatcgc tgtcagctgg tccgccgtct gaccttgcgt
      181 tgcgagcttc acgctgacat cccgctgcgc gcgttcgaat gtgctgataa aaaatggcgt
      241 gatcggttca tgaagcgatt gcgaatgaaa atccctcacc acgcctacta tagggccggc
      301 accattattc attccgatgt tgacgatttg tcccaatgcg ttcgccgggt gctgaaatcc
      361 cagctgtttt gcacaggttt cgttgaccag gaattcccgg atggtatccg cgggaaagag
      421 attccgcccc gccaccaggt gcattccgaa taacccgaga tagctcgtat ccaccatatc
      481 aaaggaggcc atcgtctttc ggttcgcatc gctgctcaat tggatgatcg ttcccggatg
      541 ccccgccgca gtgggcatct ccatatgccg ggttaccatc ttgacgccgg gtatctcccg
      601 gataaggtta gcgagcgcgg cgcgatggtg ttgcgggcca tcttcctgcg cgcggatcac
      661 gatgatcgcg tcatggtcga acccgagatc actcgtcagc acataatgga tttggcgcga
      721 aatgatcatc gtgccgatga taaagaccag cgatacggtg aattggaaga cgataagcgc
      781 cttccgcaga tagctttttc cgttcaggtg ttgaacaccc tggcccttca gactgatcgc
      841 gggtcggtat gacgacagga cacgggcagg aaagaatccc gccagaaagc tcgttacgac
      901 tacggtggcc gccaggaaaa gtgccgccgg ccattggaag acgttcaggt gcacacctct
      961 tggaagcagc gacccgaagg ccgccagcag cggatcagtg attagtaacg ccagcgttgc
     1021 agccgctatt acaagcacaa gtgtctccga caggaactgg aggattaacg cgccgcggcg
     1081 cccgccgagg accttgcgaa cgccaatctc ccgtgcacgc cgcaccgact gcgccgtcgc
     1141 aagattgatg aaattgatgg cggcaataac caggataaaa atggcgatcg ctgccaggcc
     1201 atacagcgta ggaagacttg ttttgcggga aaaggcatct gagtatttct cgttgaaatg
     1261 aatgtcctgc agcggctgca gggagatttt caccttagag tccttcggac ccacccggtg
     1321 acgcgcaaca aaggccggaa attgtttttc aatctgcgcc ggactgactc ccggcgccaa
     1381 ctttacaatc gcctgcgcat cgtaatccca catgccccag tttgtcggat cgtaatcgta
     1441 cttcaggaaa ctatggccga tcgtggcgta ggagataaag tccttaaagc caaaatcgct
     1501 cttctgcttc caatcggcga caataccgga gaccgtcacc cgcagggaat ctcgatagat
     1561 cagcggcctg cccagccatt cttccggttg cgccggaccg aagtactttt cggcttccga
     1621 tgctgagagc accactttga acggctcatt taatgccgtc gcggcgttcc cggccaacca
     1681 ctggtactgg aagatctgga aatactccgg ctgcgcgatg atgaccggcg aagtctgttc
     1741 gccccgtttg gcggcgtcaa acaccttgtt tgtctgtccc ggccgcggca cgattaccga
     1801 actggtgagg tcatcaaaag ccgaaacggt ttgcaatccg ctgatctccc gtcgcgcatc
     1861 attcggcagc cccatggtga cgaagccgaa atcccgttcg ccacccatac cgcccaatgt
     1921 ggctacgacc ctatagatcc ggtccccctc cggatgaaac cggtcatacc cgaattcgaa
     1981 ccggacaagc aggaaaatga taaggcacgc cgtgatgccc agagcaagtc cggcgatatt
     2041 gatcattgtc gtcaatttat tccgccgcat actgcgccag gcggttttga agaagttgag
     2101 gagcatcgtg gtagtatcta gggttaccgg acgtacgaaa agcatgccta tgttttaaaa
     2161 tactgattat caattattta gataagcaaa ctgctgccgc actgttcttt attgatacac
     2221 cgcctgtccg gtctgcacgg acgcaccggc aaccgcctac cccattttcc gtaattttgt
     2281 agactatgcc acgccatttg cgtcacctga tcctgctgta cccattgctg ctggttgccc
     2341 tcctgtccta cccacagcaa cggcataggg atacgcttat cctccacttc gcttttaata
     2401 gccaccgcat tccatcccag gacagcacgc ggctaagcca ggccttggcc agtgaaaaga
     2461 ttgattccgt gcaaatcgac ggctacaccg ataaggtcgg ctcggccgct tataatcttg
     2521 cgctctcgcg ccgccgggca aaagcggcaa gtcgattgat ttcccgcgag acggtggacg
     2581 attcgcgcct gcatgccgcc gggcatggca aagccccgac gacccaatgg tccgacagtg
     2641 ccaaccgccg ggttgaggtg gtcctgtttt acacgcgccc ccccgaggcc tccggaacgc
     2701 cacctgccg
//
LOCUS       DATGKE010000280         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_135939, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000280 DATGKE010000000
VERSION     DATGKE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135939"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VKU83_04845"
     CDS             <1..225
                     /locus_tag="VKU83_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92908.1"
                     /translation="LDVELDDRGNVRASDRNYQTNIAKVFTAGDMRRGQSLVVWAISE
                     GRECARKVDEYLVGASGSLLERRDQSLIQL"
     gene            complement(286..1755)
                     /locus_tag="VKU83_04850"
     CDS             complement(286..1755)
                     /locus_tag="VKU83_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI92909.1"
                     /translation="MLKELFLCSGALLAVSAGYCQEETAHPAVAAADTTRTPAKEPAS
                     EPFAFGDFSWLNGTSRKTSPPAIDTKYFTGDITLDVNYTHSFNNPVDNTVVGSTALAR
                     NNELQLSFLGFGGDLHVDNVRGRIMMQFGTRSTVVPRNDGSSYKGQYDLQTIYRYISE
                     AYAGYHWDVWHGINLDAGIFMSYIGLFSYNNFENWGYQPSYTSDNTPWFFNGLRLQTF
                     PTDKLKVELWLINGWQSYGMFNNSPGAGFSIYYRPKETVDYVANFYYGKDDEGSPGRY
                     RIHSDNSYELRYFNRKHGFVTKAAFSLTGDFGFENGDGVTPFGKGVRKQPGYLPAQNF
                     ISGMGYNRLWFGEQQKFAWTIGGGYMHNPGQYLVLAPTGYADTLFQQQTGPGSTFDAW
                     DASTSIDYMPRQNLTLKLEFVHRVVNSIDPAPGTTALAGYFAGHGGVTSPSAYTNTGG
                     YTYTSNGASIAPPLSSWGGWMPDLAKSESRVIMAVLVRF"
     gene            complement(1999..>2492)
                     /locus_tag="VKU83_04855"
     CDS             complement(1999..>2492)
                     /locus_tag="VKU83_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLI92910.1"
                     /translation="LAEVLGVSGPLAMVVAGIITGNKSRLDGMSDLSRDYLDKFWGLV
                     DNLLNALLFLLIGLEMLVIPFRPTWLFLGIASIAVVLAARFVSVWLPIRLLRLRTGFQ
                     KNSVSILTWGGLRGGLSIALALSLPPAMHGGLFLVVTYIIVLFSILVQGLTIGRVAVR
                     LRK"
BASE COUNT          587 a          734 c          670 g          501 t
ORIGIN      
        1 ctcgacgtcg aattggacga ccggggaaat gtccgcgctt cggaccggaa ctatcagacc
       61 aatattgcca aggtctttac ggccggcgac atgcggcgcg gccaatcgct cgttgtctgg
      121 gccatcagcg agggccggga atgcgcccgc aaagtggatg agtacctggt tggcgcctcc
      181 ggctcgctgc tcgaacgccg cgaccaatca ctgatccaat tgtaacgagg cagcttcgtc
      241 caggcgaagc aggtgctatc cgttagattt cgccggcacg gttcgtcaga agcgcaccaa
      301 tacggccatg atgaccctcg attccgactt cgcgaggtcg ggcatccagc caccccagct
      361 gctcaggggc ggcgcaatac ttgccccgtt gctcgtatag gtgtacccgc ccgtattggt
      421 ataggccgac ggcgatgtca cgccgccatg accggcaaaa tatcccgcca gcgccgtcgt
      481 acccggagcc ggatcgatcg aatttacgac ccggtgaaca aactccagtt tcagcgtcag
      541 gttctgacgg ggcatataat ctatcgacgt ggaagcatcc caggcatcga aggtcgatcc
      601 cggacccgtc tgttgctgga acagcgtatc cgcgtaacct gtcggcgcca ggaccaggta
      661 ctggccggga ttgtgcatat aaccgccacc gatcgtccag gcgaattttt gctgctcgcc
      721 aaaccacagc cggttataac ccatgccgct gataaagttt tgggccggca aatagccggg
      781 ttgcttccgc actcctttgc caaaaggagt tacgccatcg ccattttcaa atccaaaatc
      841 accggttagc gaaaaggcgg ccttcgtgac aaatccatgc ttcctgttga aataccggag
      901 ttcataactg ttgtcgctgt gaatccggta gcggccgggc gacccttcgt cgtccttgcc
      961 atagtagaaa ttggccacat agtccaccgt ttctttcggc ctgtaataga tcgagaaccc
     1021 cgccccgggc gaattattga acatcccgta gctctgccag ccattgatca gccacaattc
     1081 cactttcagc ttgtcggtag gaaaggtctg caaccggagc ccgttgaaga accagggcgt
     1141 attgtccgag gtatatgacg gctgatagcc ccagttctca aaattattgt atgagaacaa
     1201 cccgatataa gacatgaaaa tgccggcatc caggttgatc ccatgccaca catcccagtg
     1261 gtaccccgca taggcttcac tgatataccg gtaaatggtt tgcaggtcat attggccctt
     1321 atacgaactg ccgtcatttc gggggactac ggtagaccgc gttccgaact gcatcatgat
     1381 gcgcccgcgg acgttgtcga cgtgcaaatc gccgccaaat cccaggaaag aaagttgcaa
     1441 ttcattattc ctggccaggg ccgtggatcc gaccaccgta ttgtcgacgg ggttattaaa
     1501 ggagtgcgtg taattcacgt caagcgtgat gtcgcccgta aaatactttg tgtcgatcgc
     1561 cggcggcgag gtcttccggc tggtgccatt cagccaggaa aagtccccaa aagcaaacgg
     1621 ctcggaagcc ggctcctttg caggggtccg cgtggtatcc gctgctgcga ccgccggatg
     1681 cgccgtttcc tcctggcaat accctgcact cacggctaat agtgcgcccg aacataaaaa
     1741 taactcctta agcatagtcc tgtatttaag gtattaagtc gaagccttcc ggcccgcggc
     1801 cggaggcaat tggcatcggc caattacgta ccagaactac tttcccctgt acatgcgcag
     1861 ggtggaaccc aacactgtca agctttccgt tgactcagtc caagggacaa aggctccccg
     1921 ggccccatcc gccaaccccg aattgaaaaa aggctatcaa atcgggaggc taatggccac
     1981 aaacgcctgt gagggaagct acttccggag ccttacggcg acgcggccga tagtgagccc
     2041 ctgcaccagg atcgaaaaca ggacgataat ataggtaacc accaggaaaa gaccgccgtg
     2101 catcgccggc ggaagcgaaa gcgcgagggc aatggaaagt ccgcctcgga gtccgcccca
     2161 ggtcaggatc gagacggagt tcttttgaaa tccggtcctc agccgcagca gccggatcgg
     2221 aagccatacc gaaacgaatc gcgcggcgag caccaccgca atcgaagcta tgccgaggaa
     2281 cagccatgtg ggtcgaaaag ggattaccag catctccaaa ccgatgagta ggaagagcag
     2341 cgcgttgagg aggttgtcaa ccagccccca aaatttgtcc aggtaatccc ggctgagatc
     2401 gctcattcca tcgagccggc tcttattgcc ggtaatgatc ccggctacca ccatggccaa
     2461 tgggccgctc acacccagta cttccgcgag ca
//
LOCUS       DATGKE010000281         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_752367, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000281 DATGKE010000000
VERSION     DATGKE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752367"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VKU83_04860"
     CDS             <1..692
                     /locus_tag="VKU83_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HLI92911.1"
                     /translation="PISIPHGFGQKQDIEIAGFFAATFSWGNRTTIISKSRELMRLMD
                     NAPYQFVLHHTDKDLRKLLAFKHRTFNPTDLLYFIEFFRTHYSRYASLEDAFLLGGPG
                     WEAGAALSAFHPYFFSLEAVPPRTRKHIATPERNSSCKRLNMFLRWMVRKDPNGVDFG
                     LWHRIPVSRLVCPLDLHAARVARRFGLLTRTTTDWAAAVELTARLAMLDPRDPVKYDF
                     ALFALGALEKF"
     gene            694..1029
                     /locus_tag="VKU83_04865"
     CDS             694..1029
                     /locus_tag="VKU83_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92912.1"
                     /translation="MDLPALQNDLRSACGLDLPATSSTDQLEELLAEKINAMILADFG
                     GLVQLLYRIDVSESRLRQLLKAGAAVDAGRIVARLILERLWEKIQTRSKYNAATQKGR
                     PDSGEEERW"
     gene            complement(1048..1704)
                     /locus_tag="VKU83_04870"
     CDS             complement(1048..1704)
                     /locus_tag="VKU83_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLI92913.1"
                     /translation="MKFEKIHNKGQAQLFKNQYLEMLTKTHPLVIWGMYLPVIGYSLY
                     YGGYRYDFPFVRIALVFLGGMFFWTFFEYLMHRYVFHWVSENERAKKIIYVMHGNHHH
                     FPRDKERLFMPPVPSLIIASLLFILMRWVMGSAVFMFFPGFLLGYLLYGSMHYAIHAW
                     NPPFKWMKPLWRNHHLHHYKDEHRGFGVSSTIWDRVFGTMFDLRKEKEDKEKVKELMF
                     "
     gene            complement(1752..>2623)
                     /locus_tag="VKU83_04875"
     CDS             complement(1752..>2623)
                     /locus_tag="VKU83_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLI92914.1"
                     /translation="SSAISALPTLGQYIRKIFLVSDNDAYNRLYEFLGQESINRRLWQ
                     LGYPDIRITRRFVPMSEEENRNTNPVRFVKDGKTRYLQPAEYSGLKFDFSQQVLIGNA
                     YYDWNDSLIHAPMDFTRHNNFPLEDAQQILQSVLFPESFPASRRFRLTAADYRFLYQY
                     MSEYPRESLYPHYDSTYFDSYTKFFFFRAGHTPVPPFIRSFNKPGWSYGFLTDIAYIV
                     DFKNKVECMLSGTIYVNRDGILNDDKYEYEEVGWPFFKEVGSIIYQYELQRARAHRPD
                     LSRFVMDYGTAGH"
BASE COUNT          627 a          719 c          689 g          588 t
ORIGIN      
        1 atccgatcag cattccccac ggcttcggcc agaaacagga tatcgagatc gccggattct
       61 ttgcggccac cttttcctgg ggcaaccgga ccacgatcat ttccaaatcg agggaactga
      121 tgcgcctgat ggataatgcc ccttatcagt tcgttctgca tcataccgat aaggacctgc
      181 gaaaactgct tgcattcaaa catcgcactt tcaatcccac tgacctgctg tattttatcg
      241 aattcttccg cacccactac agccgttacg cttccctcga agacgccttc ctgttaggcg
      301 gtccgggctg ggaagccggc gctgcgcttt ccgcctttca cccttatttc ttttccctcg
      361 aagccgttcc gcccaggacc agaaaacata tcgctacgcc ggaacggaat tccagctgca
      421 agcggttgaa catgttcttg cgctggatgg tgagaaagga tccgaacggc gttgacttcg
      481 gcctgtggca tcggattcct gtcagccgcc tggtttgccc gctcgacctc catgcagccc
      541 gggtggcaag gcggtttggg ctgctcaccc gcacgacaac ggactgggcg gccgccgtcg
      601 aactgaccgc ccgcctggcc atgctggacc cacgggaccc ggtaaaatat gatttcgccc
      661 tgtttgccct gggcgcactg gaaaaattct gaaatggacc tacccgcatt acaaaatgac
      721 ctccgctccg cctgcggtct cgacctgccg gcgacaagct ccaccgatca gctcgaggag
      781 ctcctggctg aaaaaatcaa cgcgatgatc ctcgctgact tcggcggact cgtccagctt
      841 ctttaccgga ttgatgtcag cgaatcgcgg ctgcggcaac tactgaaagc gggcgctgcg
      901 gttgacgctg gaagaattgt tgccagactc attttagaaa ggctgtggga aaaaatacaa
      961 acgcgaagca aatataatgc ggccacgcaa aaaggaaggc cagactccgg ggaggaggag
     1021 cgatggtaga ggttcaatcg gcagcagtca aaacatcagc tccttcacct tctccttgtc
     1081 ctctttttct ttccggagat cgaacatcgt tccaaacacc cgatcccaga tggtgctgct
     1141 gacgccgaag ccccggtgtt cgtctttgta atggtggaga tggtggtttc gccagagcgg
     1201 cttcatccat ttaaagggcg ggttccaggc atggatcgca tagtgcatcg atccatagag
     1261 aaggtacccc aggaggaatc ccggaaagaa cataaacacc gcgctgccca tcacccagcg
     1321 catcaggatg aaaaggagcg aggcgatgat gagactgggc accggcggca tgaacaaccg
     1381 ctccttgtcg cgcgggaaat ggtggtgatt gccgtgcatc acgtaaatga tcttttttgc
     1441 ccgctcattt tccgacaccc agtgaaaaac atagcgatgc atcaggtatt cgaagaatgt
     1501 ccagaaaaac attccgccca gaaacaccag cgcgatgcga acgaacggga aatcgtaccg
     1561 gtatccgcca taatacagcg aataaccgat gacgggaaga tacatgcccc agatcaccag
     1621 gggatgggtc ttcgtaagca tttcgagata ctggttcttg aaaagctgcg cctgcccctt
     1681 attatgaatc ttttcaaatt tcatgtcgtc ccgttaggcc acaaaaataa gaagatccgg
     1741 cgaatcccga gctaatgccc ggccgttcca tagtccatga cgaagcggct cagatccgga
     1801 cggtgagccc gggcgcgctg taactcatat tgatagatga tcgagccgac ttccttgaaa
     1861 aaaggccagc cgacctcttc atattcgtat ttatcgtcat ttaagatccc atcccggttc
     1921 acgtagatcg tgccgctcaa catgcattcg accttgttct taaagtctac aatataggct
     1981 atatccgtca ggaaaccata ggaccagccg ggtttgttga agctcctgat aaagggtggg
     2041 acaggagtat gcccggcccg gaaaaagaag aatttggtat agctgtcgaa ataggtggaa
     2101 tcataatggg gatatagcga ctcccttgga tattcggaca tgtactggta aaggaagcga
     2161 tagtcggctg cggtcagccg gaagcgcctg ctggccggaa acgattccgg aaacaacacc
     2221 gattgaagga tctgctgcgc gtcttccaat ggaaaattat tatgtcgcgt aaaatccatg
     2281 ggggcatgga tcagggagtc gttccagtca taataagcgt tgccgatgag gacctgctga
     2341 ctgaaatcaa acttcagtcc gctatattcc gcgggctgca gatagcgtgt tttgccgtcc
     2401 ttcacgaacc tgaccgggtt ggtgttcctg ttctcttcct cgctcatcgg cacaaatcgc
     2461 ctcgtgatcc ggatgtcggg gtagcccagc tgccatagcc gcctgttgat cgattcctgg
     2521 ccgaggaact cgtacagccg gttgtaggcg tcgttgtcgc tgaccagaaa gattttccgg
     2581 atatactgac caagcgtcgg cagggcgctg atcgcgctcg aat
//
LOCUS       DATGKE010000282         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1496913, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000282 DATGKE010000000
VERSION     DATGKE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496913"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VKU83_04880"
     CDS             <1..1038
                     /locus_tag="VKU83_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92915.1"
                     /translation="YVASFIVLSAVIILPLIPYGFGYWFNHGQAPHSSRVSPFEIAGE
                     FFGSSQWIKFYLFLLVFLAVIRYRSRASWKADTSGILFLALTLGILAEAAVLQVTSYT
                     PPDNNIFFHSFGFSCTLVLLAPLLPVNLGRPRMAALLVAGVLLWWSGVWWKYTLRFTG
                     GHPLTGITVSATGENVVGKDNYMINRDTVTDVPTERWVECGLPAFRRITMPPATAEGI
                     HRLMKMDLVTKNKGDLKVLNMSELTPLAVEIPYKLERNGQLPLWYHLGVGMFNRQADS
                     LETRIRHHYYDLVLFEYLPTLNNFYPFRVRDSLQLHYQLIDSFMAPRRGLETRGTIEV
                     YVNSQHTDQSH"
     gene            1004..>2247
                     /locus_tag="VKU83_04885"
     CDS             1004..>2247
                     /locus_tag="VKU83_04885"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLI92916.1"
                     /translation="MSIANILTSPIEYLKGVGPMRAELLKKELGVYTFQDLLEHFPYR
                     HVDRTRINPIRDIQPDMGFVQVQGRISRVEVVGARQTRRLVAEIRDATGEMELIWFQA
                     LAGMQKLLKVGEEFLVFGRVSYYQGKPQLTHPEVEAVVPGTAGRPDFLEPVYPSTEKL
                     KARGLGGRQMAKLTRVLFDLLKEKDLPENLPASVFEYPDPVTGRPTRWLPRFEAYRQI
                     HFPTQPELYRQAVYRLKFEELFISQLRLGLIKSRRHRSSKGVVFEKVGKLFNGFYTNH
                     LPFELTGAQKRVLKEIRRDTGAGRQMNRLLQGDVGSGKTIVALLAMLLALDNGYQACL
                     MAPTEILARQHYEGISGLLGGLPVEVRLLTGSSKSKARREVLELARDGRLQILIGTHA
                     VLEESVQFARLGLAIVDEQHRFG"
BASE COUNT          452 a          595 c          721 g          479 t
ORIGIN      
        1 tacgtggcga gtttcatcgt cctttcggcg gtgatcattc tgccgctgat cccatacggt
       61 ttcgggtatt ggttcaatca cggtcaggcg ccgcattcgt cgcgcgtatc tccttttgaa
      121 attgccgggg aattcttcgg atcgtcccaa tggatcaagt tttacctttt cctgctggtc
      181 ttccttgccg tcatccggta ccgttcccgg gcgtcgtgga aggcggatac cagcggcatt
      241 ctgttcctgg cgctgaccct tggcattctg gccgaagcgg cggtattgca ggtgaccagc
      301 tacaccccgc ccgacaacaa tatctttttt cacagcttcg ggttttcatg cacgttagtc
      361 ctgctggcgc cgctattgcc ggtgaacctg gggcggcccc ggatggcggc attgctcgtc
      421 gcgggcgtgc tcttatggtg gagcggggtc tggtggaagt ataccctgcg ttttacgggc
      481 ggccacccgc tcaccggcat taccgtatcg gccaccggcg aaaatgtggt tgggaaagac
      541 aattacatga tcaaccggga tacggtgacc gatgttccaa ccgagcgctg ggtggaatgc
      601 ggcctgcccg cgttccggcg gataacgatg ccgcccgcga ccgcggaggg gatacaccgt
      661 ttgatgaaaa tggatctggt tacaaagaac aaaggcgatc tgaaggtgtt gaatatgtcg
      721 gagctgacgc cgctggctgt ggagataccg tacaaacttg aaaggaacgg gcaattgcct
      781 ttatggtatc atttgggcgt ggggatgttc aaccggcagg cggacagcct ggagaccagg
      841 atccggcacc actattatga cctggtactt ttcgaatatc tgccgacgct gaacaatttt
      901 tatccattca gggtgaggga ttccttgcag cttcactatc agcttataga ctcctttatg
      961 gccccgcggc gggggctgga gacgagggga acgatcgaag tttatgtcaa tagccaacat
     1021 actgaccagt cccattgaat atctcaaggg cgtaggaccg atgcgggcgg agcttttgaa
     1081 aaaagagctc ggcgtgtaca ccttccagga cctcctcgag catttcccgt accggcatgt
     1141 cgacaggacg cggatcaacc cgatccgtga catccagccg gacatgggat tcgtccaggt
     1201 gcagggccgg atcagcagag tggaagtcgt gggcgccagg cagacgaggc ggctggtggc
     1261 agagatcagg gatgctaccg gggagatgga actgatttgg ttccaggcgc tggcgggcat
     1321 gcagaagctg ctgaaggttg gggaggagtt cctggtattc gggcgggtga gctactatca
     1381 gggcaagcca cagctgacgc atccggaggt ggaggcggtc gtgccgggaa cggccgggcg
     1441 gccggatttc cttgagccgg tgtatccgag cacggaaaaa ttaaaggcca gggggctggg
     1501 aggccgccaa atggcgaagc tgacccgggt gcttttcgat ctcttgaagg agaaggacct
     1561 gccggagaac ctgccggctt ccgtcttcga atatccggat ccggtgaccg gcaggccgac
     1621 ccgctggctg ccaaggttcg aggcataccg ccagatccat tttccaactc agcccgaact
     1681 ttaccggcaa gccgtgtacc ggttgaaatt cgaggaattg tttatctcac agctcaggct
     1741 cggcctgatc aaatccagac gacaccgttc gtcgaagggc gtcgtgtttg aaaaggtcgg
     1801 gaagcttttc aacgggtttt ataccaatca cctgcctttc gagctgacgg gagcccagaa
     1861 gcgggtcctg aaggagatcc gccgggatac aggcgccgga aggcagatga accgccttct
     1921 ccagggcgat gtcggtagcg gtaaaacgat cgtggcgctg ctcgcgatgc tgctcgcgct
     1981 ggacaacggg tatcaggcat gcctcatggc gccgaccgag atcctggcaa gacaacatta
     2041 cgaggggatt tccgggttgc tcggcggttt gccggtggag gtccgactgc tgactgggtc
     2101 ctcgaagtca aaagcgcgca gggaggtgct ggaactggcg cgcgacggcc ggttgcagat
     2161 cctgatcggg acgcacgcgg tattggagga atccgtccaa ttcgcgcggc tcggcctggc
     2221 gatcgtcgac gagcagcacc ggttcgg
//
LOCUS       DATGKE010000283         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1054214, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000283 DATGKE010000000
VERSION     DATGKE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054214"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VKU83_04890"
     CDS             complement(<1..997)
                     /locus_tag="VKU83_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLI92917.1"
                     /translation="MEPYTNNSTRFPLLQGLSIRKQLPLLICLLLLVIILLFGAISYW
                     GMRTAALGIGQQRLRSLTEQLASLFQQSARTLRYNTQSAAGRQEIVQYLEAAPGNRAG
                     SGKALASLQKMLGDSRIVRAELRDPQGIELLHAGRPPKFPVDPFGTDSNFVGRFHTAG
                     DSLYYPIAAAVMNGGKVLGYLFQWQTIRTTQRSMAQLTELLGGKATLYLGNRDGSSWS
                     DMLHPIPNPVPSAARSAIAAYDRPGGRVMGYALPIPGTPWMTMVELSQDLILEAANRF
                     LYGMMAIGAALILAGSFIAWLISRSFTRPLSRLMLATSELAHGNYAAPVAVHRRDE"
     gene            1106..1972
                     /locus_tag="VKU83_04895"
     CDS             1106..1972
                     /locus_tag="VKU83_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLI92918.1"
                     /translation="MKLYKTKKGNLLSDGDHFWLLGGSWEELVNRSGLHAYLKGLSTG
                     AELLSPVEADALLNSQLLAPVGGSQEIWAAGVTYLRSRDARMEESKQSGGASFYDKVY
                     EAERPELFFKALPQRVAAHDEDVYIRRDSSWNVPEPELTLFINSKAEIQGYTIGNDMS
                     SRSIEGENPLYLPQAKVYQRSAALGPCLYVPAEPISPSTRIRMVIVRGGQSAYAGEVA
                     ISMIKRKLSELAQWLYRDMDFPAGSLLMTGTCLVPGNEFTLSAGDKVDITIDGVGTLS
                     NTVAYKPMHRNG"
BASE COUNT          443 a          536 c          665 g          426 t
ORIGIN      
        1 gttcgtcacg gcggtggacg gctaccggcg cggcatagtt gccgtgggcc agttcgctgg
       61 tggccagcat caggcggctc agcggccggg taaagctccg gctgatcagc caggcgataa
      121 agctgcctgc caggatgagc gccgctccga tggccatcat tccatagagg aaacggttgg
      181 ccgcttccag gatcaggtcc tgcgagagtt ccaccatggt catccagggt gtgccgggaa
      241 tgggaagggc ataacccatc acgcggccgc ccggccggtc gtaggcggcg atagcggatc
      301 tggccgcgga cgggacgggg ttggggatag gatggagcat atcggaccac gacgagccgt
      361 ctctattacc caggtaaagc gtggctttgc cgcccagcag ttcggtgagc tgcgccatgg
      421 acctttgcgt ggtccggatc gtctgccatt ggaacagata acccaggacc ttaccgccgt
      481 tcatcaccgc ggcggcgatg ggataataga ggctgtcgcc ggcggtatgg aagcgtccga
      541 cgaaattgct atcggtaccg aagggatcga ccgggaattt cgggggtctg ccggcatgca
      601 gcagctcaat gccttgagga tcgcggagct cagcgcggac gatacgggaa tcgccgagca
      661 ttttttgaag ggaggcaagg gctttgccgg agccggcccg gttgcctggg gctgcttcca
      721 gatactgtac gatctcctgc cggccggccg cggactgggt gttgtagcgc agggtacggg
      781 ccgactgctg aaaaagggag gccagctgtt cggtcaggct ccggagccgt tgctggccga
      841 tgccgagcgc ggcggtgcgc ataccccagt aagagatggc gccgaaaagt aggatgatca
      901 ccaataaaag caggcagatc agtaaaggca gctgctttcg gatcgaaagg ccctgtaata
      961 aagggaagcg agttgaatta ttcgtatagg gttccatgtc agcacgaggt gacaggtcgc
     1021 ttttttgggt cgtgaaagct tttataatgt acgaaaaatc tttctaattg ttaaataatt
     1081 atatttggcg cctaagcacc gacatatgaa actatataag accaagaaag gcaatctatt
     1141 aagcgatggg gatcatttct ggctgttagg cgggtcctgg gaagaacttg tcaatcgcag
     1201 cggtctccat gcctatttga agggtctttc gaccggtgcc gaactactta gccctgtgga
     1261 ggccgatgcg ctgctaaaca gccagctttt ggctccggta ggcgggtcgc aggagatctg
     1321 ggcggccggt gtcacctatc tgcgcagtcg ggatgcccgg atggaggagt ccaaacagtc
     1381 ggggggagcg agcttttatg acaaggtata cgaagccgag cggcccgaac ttttcttcaa
     1441 ggccctgccg cagcgggtag ccgcgcatga cgaagacgtt tatatccggc gcgattccag
     1501 ctggaatgtc cctgagcccg agctaacgct ttttatcaat agcaaagcgg agatccaggg
     1561 gtatacgatc ggcaacgata tgagttcgcg cagcatcgaa ggggagaacc cgctctatct
     1621 gcctcaggcc aaggtctatc agcggagtgc agcgctgggg ccctgtttat acgtaccagc
     1681 cgaaccgatc tcccccagca cccggatccg catggttatt gtccgtgggg gccagagcgc
     1741 ctatgccggt gaggtagcca tcagcatgat caagcggaaa ctgtccgagc tggcgcaatg
     1801 gctttaccgg gatatggact ttcctgccgg cagtctgctc atgacgggaa cctgtttggt
     1861 gccgggaaat gaatttacgc tatcggccgg cgataaggtg gatatcacga tcgacggcgt
     1921 cggtacgctc agcaataccg tggcgtataa gccgatgcat aggaacgggt gatcattttt
     1981 taccatggat tggcagcaat tatatagccc tgttcgaacc ggatcgtccg tgccggtgga
     2041 tgccgcgcgc attgccgacc cgcagatccc
//
LOCUS       DATGKE010000284         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1254200, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000284 DATGKE010000000
VERSION     DATGKE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254200"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VKU83_04900"
     CDS             <1..556
                     /locus_tag="VKU83_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HLI92919.1"
                     /translation="RAAGTGSLGVKRYVFLLRNRKDPKKHLLIDMKQAVPSSLQPWLT
                     NPQPAWASEGERIVSVQERMQNINPALLSVTEFQGQSFVVKEMQPTADKIDFLLIRDR
                     YKDIACVIGDMAFLTASAMLRSAGRQGAALPDELIAFGADTHWQQGLLDYAAGYAAQV
                     KKDYSAYFKDFKAGFFALAGPKTK"
     gene            558..1208
                     /locus_tag="VKU83_04905"
     CDS             558..1208
                     /locus_tag="VKU83_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLI92920.1"
                     /translation="MNMALPTDIVIKLILAALIGGILGAEREYRSKSAGFRTLTLICL
                     GATIFTIFSIIIGGTGNPDRIASNIVTGIGFVGAGVIFKGDALAKVSGITTAAMIWVT
                     AALGMAIGAGYLLVSLVATCLILVVLFVFKVLEGWIDRVNQIRTYRIICDFEHDSLHR
                     YESLFISHHLKFRRNGERKTADNMITGQWVVQGTEENHRHCIHEVLRDPAVREFEF"
     gene            complement(1263..2132)
                     /locus_tag="VKU83_04910"
     CDS             complement(1263..2132)
                     /locus_tag="VKU83_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HLI92921.1"
                     /translation="MYTIENHDLNVRIHPKGAELQSIYGKHTGLEYLWGGDPAFWARH
                     SPLLFPIVGALKCNTYYFEGKAYTLPRHGFARDKEFTVQAQSGDSIQFLLTSDQQTLA
                     VYPFEFELRVSYQLRPVGLATTYQVQNRSASPIYFSLGAHPAFRVPLVPGTAYSDYYL
                     EFDGVQNAPRWPISKDGLIEREPQPLLQGTAVLRLDKQLFQKDALVFKQLVSTGVTLR
                     SGRTPRGLRIDFPGFPYLGIWDAPGADFVCIEPWCGIADSVGSDQQLATKEGINRLES
                     GAVFERSWALRLL"
     gene            2237..3484
                     /locus_tag="VKU83_04915"
     CDS             2237..3484
                     /locus_tag="VKU83_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92922.1"
                     /translation="MYRYLEIVWTSLIMALQEFRSNKLRTFLSLLGITFGIFCIISVL
                     STIASMQQAVNNDLKAFGTKTIYIDKWQYSGGPDFPWWKYIKRPVPKLSEAKELRLKV
                     SHAQYIDFDVDNQGNVDYGDNSLSGVHYYGNTEDFDKIEQVTIGAGRYFQQSDFDRGT
                     PFIVIGYQIAERLFSQPQRAVGKAVKLNDGKLAIVIGVVQKQGQSLIGGWDFDNSILL
                     TLNFMRQIYPVDYSNPKILVKGDSLITSYALGDELRGAMRSIRKLSPTEEDNFAVNNI
                     DNLSKFLDPVFSGMNIGGWAIAALSLVVGMFGVANIMFVTVRERTSQIGLKKAIGAKR
                     GVILTEFLMESAFLCIIGGLIGMIAVFILTLVFSALFSFHVFIPLNIIILAVFICLFT
                     GVSAGIIPAFVAARMDPVVAIRS"
BASE COUNT          768 a         1017 c          935 g          792 t
ORIGIN      
        1 ccgggcggcc ggcacgggaa gcctgggggt aaagcgctac gtcttcctgt tgaggaacag
       61 gaaggatcca aagaaacacc tgctcatcga catgaagcag gccgtccctt cttccctgca
      121 gccctggtta acgaatcccc agccggcgtg ggcgtcggaa ggggaacgga ttgtgtcggt
      181 ccaggagcgc atgcaaaata ttaatcccgc cctgctgagc gtcacggagt ttcaagggca
      241 gtcgtttgtc gtcaaagaga tgcagcctac cgccgacaag atcgacttcc tcctcatccg
      301 cgaccggtat aaggatatcg cctgcgtgat cggggatatg gccttcctta ccgcgagcgc
      361 gatgctccgc agcgccggcc gccagggcgc cgccctgccg gatgaactga tcgcatttgg
      421 tgcagacacc cactggcaac agggactgct cgactatgca gcgggttacg cggctcaggt
      481 aaagaaggac tacagcgcct attttaagga cttcaaggcc ggctttttcg cccttgccgg
      541 accaaaaacg aaatagcatg aacatggcat tgcccacgga tattgtcatc aaacttatcc
      601 tcgcagcgct catcggcggc attctcggcg cagaaagaga ataccgcagc aaatcggccg
      661 gtttccggac gctcaccctg atctgcctcg gcgcgacaat cttcactatt ttttccatca
      721 tcatcggcgg tacaggcaac ccggaccgca tcgcctccaa tatcgtaacc ggcatcggtt
      781 tcgtgggcgc aggcgtcatt ttcaaaggtg acgcactggc caaggtgagc ggaatcacca
      841 ccgcggccat gatctgggta acagcagccc tgggaatggc catcggggcg ggctacctgc
      901 ttgtttctct ggttgccacc tgccttatac ttgtggttct attcgtcttt aaagttttag
      961 aaggctggat cgaccgggtc aaccagatcc gtacctacag gatcatttgc gatttcgaac
     1021 acgactccct tcaccgttat gaaagtcttt ttatatccca tcacctcaag ttccggcgga
     1081 atggtgaacg gaaaacagca gacaacatga tcaccggtca gtgggtagtc cagggcaccg
     1141 aagagaacca ccggcactgc atccacgaag tgctccgcga ccctgcggtg cgggaatttg
     1201 aattctaacc ctgcccgcct tcggccgccg gagcgttagc ggaggcggct ctcagccacc
     1261 ctttacagga gcctcagcgc ccagctccgc tcgaagacag ctccactctc cagccggttg
     1321 atcccttctt tcgttgccaa ttgctgatcg ctgccaacgc tatccgcaat cccgcaccag
     1381 ggctctatac agacgaaatc ggcccccggc gcatcccaga tgcccagata aggaaagcct
     1441 ggaaagtcta tcctcagtcc ccgcggcgtc cgtcccgacc gcagggttac gcctgtggag
     1501 acaagctgct tgaaaacaag ggcatctttt tgaaaaagct gtttatcgag ccggagaact
     1561 gccgtgcctt gcaacaacgg ctgcggctcc cgttcgatca gcccgtcctt tgagatcggc
     1621 cagcggggcg cgttctggac gccgtcgaac tccaggtaat agtcgctata cgccgtcccg
     1681 ggcaccagcg gcacgcgaaa ggcagggtgc gcgcccaggg aaaaatatat cgggcttgcg
     1741 gagcgatttt gcacctggta ggtcgtggcc agccctaccg gccgaagctg gtagctaacc
     1801 ctgagctcaa attcgaacgg ataaaccgca agggtctgct ggtcgctggt caaaaggaat
     1861 tggatcgagt caccggactg tgcttgcacc gtaaattcct tgtcccgggc aaatccatgc
     1921 ctcggcagcg tgtacgcctt tccctcaaaa tagtaggtat tgcacttcaa ggcgcccacg
     1981 atagggaaca acaatggcga atgtctggcc caaaatgccg gatcgccgcc ccacaggtac
     2041 tccagcccgg tatgttttcc gtaaatactt tggagttccg ctccctttgg atggatcctg
     2101 acgttcagat catggttttc gatcgtatac attcgatttg ttttatcggc gtccggcaat
     2161 ttaccgcttg acagcttttc gcgcaattta gcgtaattcc agttcccggt tggccacaaa
     2221 tgcttacatt cgagacatgt accggtacct ggagatcgtc tggacgagcc tgattatggc
     2281 cctgcaggag ttccggagca ataagctccg gaccttcctt tcactgctcg gtatcacgtt
     2341 tggcatcttc tgcatcatca gtgttttgtc cacgatagcc agcatgcagc aggccgtcaa
     2401 caacgacctg aaagccttcg gaacaaaaac catctacatc gacaaatggc aatattccgg
     2461 cggtcccgac tttccctggt ggaaatatat caagcggccg gtgccaaagc tctcggaggc
     2521 aaaagaactt cggctgaagg tgtcgcacgc acagtatatc gattttgacg ttgacaacca
     2581 gggaaacgtc gactacggcg acaattcgct atccggcgtg cattactacg gcaataccga
     2641 ggacttcgac aaaattgagc aggtaaccat tggcgcgggg cgctattttc agcaatccga
     2701 cttcgatcgc ggcaccccct ttatcgtcat cgggtaccag atcgcagaac ggcttttctc
     2761 gcaaccccag cgtgcggttg gcaaggcggt taaactcaac gacggcaagc tggccatcgt
     2821 catcggcgtc gtccagaaac aggggcagag ccttattgga ggatgggatt tcgacaacag
     2881 cattctcctg acgctgaatt ttatgcgcca gatttacccc gtcgattaca gcaacccaaa
     2941 gatcctggtg aagggtgact cgctgattac ttcctatgcg cttggagatg agctccgcgg
     3001 cgcgatgcga tccatccgaa agctgagtcc cacagaggag gacaattttg cggttaataa
     3061 tatcgataat ctaagcaaat tcctggaccc cgtcttctca ggcatgaata tcggcggctg
     3121 ggccatcgcc gcactctcgc tcgtcgtcgg aatgttcggg gtggcgaaca tcatgttcgt
     3181 cacggttcgc gagcggacgt cgcagatcgg gttaaagaag gccatcgggg ccaagcgggg
     3241 cgtaatcctg acggagttcc tgatggaatc cgcttttttg tgcatcatcg ggggactgat
     3301 cgggatgatc gccgtattca tcctgaccct tgttttctcc gcgttgttca gttttcatgt
     3361 cttcattccg ctaaatatca tcattctcgc cgttttcatt tgtcttttta ccggagtatc
     3421 ggcgggcatt attcccgcct ttgtcgccgc ccgcatggat cccgttgtcg ccatcaggtc
     3481 ctgatggacc ggcaggcaca tttcctggtc cg
//
LOCUS       DATGKE010000285         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1256458, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000285 DATGKE010000000
VERSION     DATGKE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256458"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VKU83_04920"
     CDS             <1..1068
                     /locus_tag="VKU83_04920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HLI92923.1"
                     /translation="INVAEMKKLFDMPDARKVHHSPITPLGGLGIFAGFVFGCLLTVH
                     FNEAAELQYFIGASLVIFFLGLKDDILVISPIKKFIGQVLAAFIIIYYGNIQIRSMDG
                     FLGIYQLPEMFSLLLTYFAVIVVINSFNLIDGVDGLAGSLGLLSTTIFGAYFLHVGMP
                     SYAVLAFSLAGSLLAFLIFNFQPAKIFMGDTGSLLVGTINAILVVKFINVANSSGVSM
                     PINASPAVGFTILMIPLLDTLRVFGIRILHRRPPFSPDRNHIHHLLLDRGFSHRAITL
                     VLVTINMLMIGMIFFARSLNCTLLIFSVFLVFFLIIAGIYYLMPAPRLFVAKTRNEEN
                     AQLTKASQFIALKDSILEQNN"
     gene            1175..1738
                     /gene="frr"
                     /locus_tag="VKU83_04925"
     CDS             1175..1738
                     /gene="frr"
                     /locus_tag="VKU83_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLI92924.1"
                     /translation="MSDDLELILEDARDSMKKALNHLEGELVKIRAGKASPQMLEGLT
                     VDYYGSPTVLNQVGNISVMDARTLTIQPWEKNMLQPIERAIINANLGVTPQNDGNIIR
                     LFMPPLTEERRREFVRRASGEGEQSRVAIRNIRREAIEAIKKLKNDGLSEDEAKDAEK
                     EIQEITDRHIGLVDKHLAAKEKEIMAV"
     gene            1894..>2223
                     /gene="mscL"
                     /locus_tag="VKU83_04930"
     CDS             1894..>2223
                     /gene="mscL"
                     /locus_tag="VKU83_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HLI92925.1"
                     /translation="MSLLKDFKGFIMKGNVVDLAVAVIVGAAFSAIVSSLVDNVITPL
                     ILTPALKFAHLQDIDGLKWGAVKYGLFLAAILKFLVFGFILFLIIRGMHSFEKKKDAA
                     PPPPTTTE"
BASE COUNT          502 a          610 c          551 g          560 t
ORIGIN      
        1 atcaatgtgg cggaaatgaa aaagctgttc gatatgcccg acgcgcggaa agttcaccat
       61 tctcccatta cgccgctcgg cggtctcggc atctttgccg gcttcgtatt cggttgcctg
      121 ttaacggtgc attttaatga agctgctgaa cttcaatact ttattggggc aagcctggtc
      181 atcttcttcc ttggattgaa agacgatatc ctcgtaatct ccccgatcaa gaaatttatc
      241 ggccaggtgt tggcggcgtt catcatcatt tattatggaa atatccagat ccggagcatg
      301 gatgggtttc tgggtatcta tcagcttccg gaaatgttca gtcttttgct gacttatttt
      361 gcggtcatcg tcgtcatcaa ctcctttaat ctgatcgacg gggtggacgg actggccgga
      421 agcctggggc ttttgtctac gaccattttt ggcgcttatt ttctccatgt cgggatgcca
      481 tcctacgcgg tgctggcctt ttcattggca ggcagcctgt tggccttcct gatcttcaac
      541 tttcaacctg cgaagatctt catgggcgac accggctccc tcctcgtcgg gacgatcaat
      601 gccattctcg tcgtgaaatt tatcaacgtc gccaatagtt ccggcgtcag catgccgatc
      661 aacgcatcgc cggccgtcgg tttcaccatc ctgatgatcc cgctgctcga tacgctccgg
      721 gtcttcggca tccgtatcct tcaccggcgt ccgccgttca gcccggacag aaatcatatc
      781 caccacctcc tgcttgaccg cgggttctcc catcgcgcca tcacactcgt cctggtgacg
      841 atcaatatgc tgatgatcgg catgatattc ttcgcccgat cgttgaattg caccttgctc
      901 attttttccg ttttcctcgt ttttttcctt atcatcgcgg ggatctacta cctcatgccc
      961 gccccccggc tgtttgtggc aaagacacgc aacgaggaga atgcgcagct gacgaaggcc
     1021 tcgcagttca tcgccctcaa ggactccatc ctggaacaaa ataattaaat tgccccaaaa
     1081 tttttgttgc gattctgggt acatttaaag tatgtttgta cggtcattga accggccggg
     1141 gccggttcgc ctgactttca tttataactt gagtatgtca gacgatcttg agctgatcct
     1201 ggaagatgcc cgggacagca tgaaaaaagc gctgaatcat ctggaaggtg aactggttaa
     1261 gatcagggcc ggtaaagcca gcccgcaaat gctggaaggc ctgaccgtgg actattacgg
     1321 cagtcccacc gtcctgaacc aggtagggaa tatctccgta atggacgccc gcaccctgac
     1381 catccagccc tgggaaaaaa atatgctgca gcccatcgaa agggcgatca tcaatgccaa
     1441 cctgggcgtt acgccgcaga atgacgggaa catcatccgc ctgttcatgc ctccgctaac
     1501 cgaagaacga agaagagaat ttgtccgccg cgcgtccggt gaaggcgagc aatcccgggt
     1561 cgccattcgc aatatccgcc gggaggcgat cgaggcgatc aagaagctga agaacgacgg
     1621 tctcagcgaa gacgaagcga aggatgccga aaaggagatc caggaaatca ccgacaggca
     1681 tatcgggctg gtcgacaagc atctggccgc aaaagaaaaa gagatcatgg ccgtatagga
     1741 gtatccgtcc ccatcttatt catttcacga gaaccttatg gattcccatt gaggctgcca
     1801 tagccgcctc cattcgtccc agcgctgtcc tatctgtaac ggtgaggagc acgtcaggca
     1861 tgttttttta ataaccaact aaaaacctcg gttatgagtt tactcaaaga cttcaaggga
     1921 tttatcatga aaggcaatgt ggttgacctg gccgtagccg taattgtcgg cgccgccttt
     1981 tccgccatcg tctcttccct ggtcgacaac gtgattactc cgctgatatt gacgcctgca
     2041 ctgaagtttg cccaccttca ggacatcgac ggcctgaaat ggggcgcggt caaatacggg
     2101 cttttccttg cggcgatcct taagttcctg gtcttcgggt ttattctctt tctgatcatc
     2161 agggggatgc acagctttga aaaaaagaag gatgcggcgc ccccgccccc gacaacgacc
     2221 gaa
//
LOCUS       DATGKE010000286         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_846714, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000286 DATGKE010000000
VERSION     DATGKE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846714"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..784)
                     /gene="pxpB"
                     /locus_tag="VKU83_04935"
     CDS             complement(86..784)
                     /gene="pxpB"
                     /locus_tag="VKU83_04935"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HLI92926.1"
                     /translation="MQGHTTPPSIYPLGDSAITLDLGNGIGPQLNSRALAIYAYISEH
                     RFPGLLDIIVAYSSVSVLFDPARIGDRSAVADLLWQAWQRTDGNNDPPEGRLVRIPVC
                     YEAEYGPDLEAVARQKEISPDELVMLHCSTVYRIYMIGFLPGFPYLGRLDKRLNVPRK
                     LRPVPVLAGGVGIAGMQTGIYPLNSPGGWQIIGRTPLGLFDRDADPPVLLSIGDRVEF
                     YRVSAAEFVSWKSD"
     gene            950..>2327
                     /gene="topA"
                     /locus_tag="VKU83_04940"
     CDS             950..>2327
                     /gene="topA"
                     /locus_tag="VKU83_04940"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HLI92927.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGEEFEVRSCYGHIRDLEKDDMGI
                     DVKDNYRPRYIVPEDKAKIVKELKTATKKSLEVWLATDEDREGEAISWHLCEVLGLDP
                     LHTKRIVFHEITKPAIQSAVTRPRTVDMNLVNAQQARRVLDRIVGFELSPVLWRKMSM
                     KNNLSAGRVQSVAVRIIAEREREINAFVPVSSFRVEALFTARDLGGKPVSFLAEGKKQ
                     SSPEDARAFLESCIGADYTVRDIQVKPGKKTPAAPFTTSTLQQEASRKLGYGVARTMI
                     LAQKLYESGKITYMRTDSVNLSDLAMDAISHQIRNQYGERYIQARRYRNKNESAQEAH
                     EAIRPTDMGSLTVEDADCRRLYDLIWKRTMASQMADAELEKTVAKIAISTRANTQGPA
                     GTASASPGDELTASGEVLKFDGFLKVYREDRDDDDVSEEDGHEGMLPPLTTGQRLPLK
                     EMRAVERFT"
BASE COUNT          618 a          629 c          652 g          428 t
ORIGIN      
        1 aatggaaaaa gacatagtgt atgtttacag gtgcagaaaa gagggcagat caggcaacca
       61 gagtgtctaa tgtaaggaaa atttattaat ccgatttcca ggatacaaat tccgcagcgg
      121 aaacccggta aaactcgacc cggtcgccga tggaaagcag gaccggcgga tcggcatccc
      181 ggtcaaacag cccaaggggg gttcgaccga tgatctgcca gcctcccgga ctgttgagcg
      241 gataaatacc ggtctgcatt ccagcaattc ctacaccacc ggccaatacc ggaaccggac
      301 gcaatttccg gggcacattc aaccgcttgt ccaatctacc caggtagggg aacccgggaa
      361 gaaaaccgat catatagatc cggtaaacag tggagcaatg aagcatgacc aattcgtccg
      421 gggatatttc cttttgtcgg gcaaccgctt ccagatccgg accgtattcc gcttcataac
      481 aaaccgggat ccgaacgagg cggccttccg gcgggtcgtt gtttccatcg gtccgttgcc
      541 aggcctgcca aagcaaatcc gccacggccg aacgatcccc aattctcgcc ggatcgaaca
      601 gcaccgatac cgaagagtag gccacgatga tatccaatag gcccggaaaa cggtgttcgc
      661 taatgtaggc atagatcgca agcgcccgcg aattgagttg aggcccaatg ccattaccca
      721 ggtccaacgt gatggctgag tcgcccaggg gatagatgga cggcggtgtg gtgtgccctt
      781 gcattcataa atgtataact ttcgatggat actggcatat aaatcgctgg aatacagcaa
      841 ccagcaattt tagggaactg aattgccccg acgcttgcaa cctggttatt ttttagtctg
      901 gcggttatac tgtaaaattg caaccaatca tcaaccggtt tttcaacaaa tggcgaagaa
      961 tttattgatc gtggagtctc cggcaaaggc caaaacgata gaaaaaatcc tgggcgagga
     1021 attcgaggtt agaagctgtt atggtcatat ccgcgacctg gagaaagacg atatggggat
     1081 cgacgtcaag gataactatc gcccccggta tattgttcca gaggataaag caaagatagt
     1141 caaggaatta aagacagcaa ctaaaaaatc acttgaagta tggctggcga ccgatgagga
     1201 ccgcgaaggc gaagccatct cctggcacct ttgcgaagtg ctcgggctcg acccactcca
     1261 taccaaaagg atcgtctttc acgagatcac caaaccggcg atccagtcgg cagtgacccg
     1321 gccacgcacc gtggacatga acctggtaaa cgcgcagcag gcgcgccggg tacttgaccg
     1381 gatagtaggt ttcgagctta gcccggtcct ctggcggaaa atgagcatga agaacaacct
     1441 cagcgccggc cgggtgcaga gtgtggccgt gagaatcatc gcggaacggg aacgggagat
     1501 caatgcattt gtaccggtga gcagcttcag ggtagaggcc ctttttaccg ccagggacct
     1561 gggtggcaag cctgtcagct tcctggctga aggaaagaaa caatccagtc ccgaggatgc
     1621 ccgcgccttc ctggagtcct gtatcggggc cgactatacg gtccgggata tccaggtgaa
     1681 accaggtaaa aagaccccgg ccgcgccatt caccacgtcc acccttcagc aggaggccag
     1741 ccgaaaactc ggctacggcg tcgcgaggac catgatcctg gcccagaagc tgtacgaaag
     1801 cggtaagatc acctatatgc ggaccgacag cgtgaacctg agcgacctgg ctatggacgc
     1861 catcagccac cagatccgca atcagtacgg agagcgctat attcaggcgc gtcggtaccg
     1921 caacaagaac gaatccgcgc aggaggcgca tgaggccatc cggcctaccg atatgggcag
     1981 cctgacggtc gaagacgccg attgccggcg gctttacgat ctgatctgga agcggaccat
     2041 ggcgagccag atggccgatg cggaactgga aaagacggtg gcaaagatcg cgatctccac
     2101 ccgggccaat acccagggtc ccgccggcac ggcttcagcg agccctggcg acgaactgac
     2161 tgcgagcgga gaagtcctca aattcgacgg cttcctgaaa gtctaccgtg aagaccgcga
     2221 cgatgacgac gtaagcgaag aggatggcca tgaaggcatg cttcccccat tgaccaccgg
     2281 gcagcgcctg cctttaaagg aaatgcgggc cgtcgaacga tttaccc
//
LOCUS       DATGKE010000287         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_390907, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000287 DATGKE010000000
VERSION     DATGKE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390907"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VKU83_04945"
     CDS             <1..1174
                     /locus_tag="VKU83_04945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HLI92928.1"
                     /translation="MTKVFLKKKISRRVEGGHPWIFANEAGVVEGDVTGGDVVEVFSH
                     DGKWMGRGYINPKSQILVRLLTREKGEAIDDGFFLFRLRRAWEYRQRIGYTENCRLVF
                     GEADDLPALIIDKFNEYFVVQTLALGMDRWKPAIVGALETIFRPKGIYERNDVPVREL
                     EGLPLQKGYLSAPFDPRIGITENGLKMEVDLENGQKTGYFLDQHDNRRAIRHIVKGAD
                     VLGAFCYTGSFETHAGFYGAKSVLGLDISEAAVTLARRNAALNGLEQICRYEAVNAFD
                     VLKKWSKEGRQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKLIKRGGFLVTSSC
                     TNLVGPELFLETIQLAARDAKRRLRQVTFRAQASDHPIVEGWENTNYLKFLIVQVM"
     gene            complement(1190..1531)
                     /locus_tag="VKU83_04950"
     CDS             complement(1190..1531)
                     /locus_tag="VKU83_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI92929.1"
                     /translation="MRIFYILLSIILLNTTRAECQAKGPLPDTDHKVVKKFYPNPAVS
                     YIIFDIDMQPNESYRLQIYSFLGKLVKDIPNMADHNTVNLADLTRGLYTFQVRDQSGR
                     ITDSGLFQLNK"
     gene            complement(1657..2136)
                     /locus_tag="VKU83_04955"
     CDS             complement(1657..2136)
                     /locus_tag="VKU83_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLI92930.1"
                     /translation="MSTNLIQIGQPAPEFSLYSSDKQKISLSDFKGKSVVLLFFPQAF
                     TGTCTKELCSTRDNIAAYNHANAQVLAISVDSLFTLARFKEDQRLNFPLLSDFNKTVS
                     ATYGCLYDNFVFDMHGVSKRSAFVVDKQGIIRYAEVLETATDLPDFEAVQKTLESIK"
     gene            complement(2165..3190)
                     /locus_tag="VKU83_04960"
     CDS             complement(2165..3190)
                     /locus_tag="VKU83_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HLI92931.1"
                     /translation="MKTLAVILVLTGALLLPAFRTRAQDIHFSQFYEAPLLRNPALGG
                     IFTGDYRISTLFRDQWNSINNAYRTGTLSGEYKWAIGHANDYLTTGLEVLYDKAGTAA
                     LTTTEAMPALNYHKSLSNIKPMYLSLGFMGGVVQKTLDMSKVTTNSQYNGTAYDPALP
                     NGETIVTPSIHYLDGALGISFNTAFGADYKNTLYVGAAYFHLNRPKNSFYKDPAVELD
                     PKVVISAGVHTIINDYLGFTIEADHSQQGSAAETIGGVMLSYKLGDDGDNPDCTFNLG
                     SYLRWGDAIVPVIRLESNHLSVGLSYDVNISSLATVSEGRGGFELSLSYIGFLEHENP
                     RYLCPKF"
     gene            complement(3231..>3993)
                     /locus_tag="VKU83_04965"
     CDS             complement(3231..>3993)
                     /locus_tag="VKU83_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLI92932.1"
                     /translation="VSDTGNLCQGLVQFQGKLVVPEVDTVFWNWNFSNGQTATGQNPL
                     VKLNSGTYTVNLKAYVSFGCSDTASGTVTINPNPTIKGPKQISTPVGVPVAIPFTYSP
                     DVNAWIWTPAANLSCIDCANPTATLTFDQTYSVLVTDSNNCTDTASILIKTVCNEGNY
                     FVPNTFSPNGDGVNDYFYPRGTSLYNIQSMTVFNRWGQMVFQRKNFPANSETMGWDGT
                     FNGKPAPADAYVYIVEVICNNAQVIALRGTVTLIR"
BASE COUNT          936 a          936 c         1206 g          915 t
ORIGIN      
        1 catgacgaag gtatttctga agaagaagat cagcaggcga gtggagggcg ggcatccctg
       61 gatcttcgcc aacgaggccg gggttgtgga aggcgacgtc accggcgggg acgtcgtcga
      121 agtgtttagc cacgacggca agtggatggg caggggctat atcaacccga aatcacagat
      181 cctcgtgcgt ttgctgacgc gcgaaaaggg cgaagcgatc gacgatgggt tctttctatt
      241 ccgcctccgg cgggcctggg aataccggca gcggatcggg tacacggaaa attgccggct
      301 ggtattcggc gaggcggatg atctgccggc gctgatcatc gacaaattca acgaatattt
      361 tgttgtccaa accctggcgc tgggcatgga ccggtggaag cccgcgatcg taggggccct
      421 ggaaacgatc ttccggccaa agggcatcta tgagcggaac gatgttccgg tcagggagct
      481 ggaagggtta ccattgcaaa aaggctattt atcggcgccc tttgatccca ggatcggcat
      541 cacggaaaac gggttgaaga tggaggtgga cctggagaat gggcagaaga cgggctattt
      601 tcttgaccag catgataatc gccgggctat ccggcacatc gtcaagggcg ccgatgtgct
      661 tggcgcgttc tgttatacgg gaagcttcga aacccacgcg gggttttacg gggcgaaatc
      721 ggtgctgggc ctggacattt cagaggccgc cgtcaccctg gccaggcgca atgccgcgtt
      781 gaacgggttg gaacaaatct gtcggtatga ggccgtgaat gcattcgacg tgctgaagaa
      841 atggagcaag gaaggccggc aatatgacgt tgtcatgctc gatccgccgg cgttcacgaa
      901 gagtcgcgaa acgatacaaa aggcgatcac cggatataag gagattaatc taaggggcat
      961 gaaactgata aagcgggggg gattcctggt gacctcctcc tgcacgaacc ttgtcggtcc
     1021 cgagttgttt ttggagacca tccaactggc ggcccgggac gcgaagaggc ggctgcggca
     1081 ggtgactttt cgggcgcagg cgagtgacca cccaattgtc gaaggatggg aaaatacaaa
     1141 ttacctcaaa ttcctgatcg tccaggtaat gtagccgggc cagccgcgct tatttattca
     1201 gctggaacag gccggagtcg gttatgcggc cggactggtc acggacctgg aaggtataaa
     1261 ggccgcgggt caggtccgca agattgacgg tgttatggtc ggccatattg ggaatatcct
     1321 ttaccagctt gcctaagaag ctataaatct gcaacctgta actctcgttc ggctgcatgt
     1381 caatgtcgaa aatgatatag gaaacagcgg ggttcgggta gaactttttg acaaccttat
     1441 ggtcggtatc cggtaatggg cccttcgcct ggcattcagc ccgggtagta ttcagtagaa
     1501 taatggataa aagaatgtaa aaaatcctca taccagggta attacagggc ttgccaaaat
     1561 aatcactttg tacctaatat acaaaaaaga tgcctaacaa aatgctaaat ttaaagttag
     1621 gcatcaaata caattaatca acgaattgat tatcaatcat ttgatggact cgagcgtctt
     1681 ttggaccgcc tcgaaatccg gaaggtctgt agcggtttca agaacctccg cgtagcgaat
     1741 aatcccctgt ttgtccacca caaaagcgga gcgtttggac accccgtgca tatcaaatac
     1801 gaaattgtcg tagaggcacc cgtaggttgc agagacggtc ttattaaaat cgctgagaag
     1861 cgggaaattg aggcgttggt cctccttgaa cctggccagc gtaaacaggg agtccacgct
     1921 gatcgccaag acctgggcat tggcatggtt gtatgcggcg atattatccc gggtgctgca
     1981 aagctccttg gtgcaggttc ccgtgaaggc ctgggggaag aacaacaata caacggattt
     2041 tcctttgaag tcgctcaggg atatcttttg tttgtccgag ctgtagaggc tgaattcggg
     2101 cgcgggctgg ccgatctgga tcaggttggt actcattgat tcgttattta gacggcaaat
     2161 ttactcagaa tttgggacac aggtaccgtg ggttttcgtg ttcgaggaaa ccaatgtaag
     2221 aaagagagag ctcgaagccg ccgcggccct cgctaacggt ggcgagtgag gagatattca
     2281 catcgtagct gagaccgaca ctcaggtggt tggattcaag gcgaatgacg ggaacgatcg
     2341 catcacccca tcgcagatac gatccgaggt tgaaagtgca gtcggggttg tcgccatcgt
     2401 cgcccagttt gtaggaaagc atgacgccgc caatcgtttc ggccgcgctg ccctgctgtg
     2461 aatggtcggc ttcgatcgta aagccgagat aatcattgat gatggtgtgc acgccggccg
     2521 agatcaccac ctttgggtcc agctcgacgg ctggatcttt atagaacgaa tttttaggcc
     2581 ggttaagatg gaaataggca gcgccgacgt ataaagtgtt tttataatcg gccccaaatg
     2641 ccgtgttgaa gctgatgccc agcgccccgt cgaggtaatg gatactcggc gtgacgatgg
     2701 tttcgccgtt gggcagggcg ggatcataag cggtcccgtt gtattggctg ttggtggtca
     2761 ctttcgacat atccagtgtt ttttgcacca ctccacccat gaatcccagc gagaggtaca
     2821 tcggcttaat attactgaga gacttgtggt agttgagcgc cggcatcgct tcggtagtgg
     2881 tgagggcggc ggtccctgct ttgtcgtaga ggacttcgag gccggtggta aggtaatcgt
     2941 tggcatggcc gatggcccat ttgtattcgc cgctcagggt gcccgtccga taagcattgt
     3001 tgatactgtt ccactggtcc cggaagagcg tcgagatgcg gtagtcgccc gtgaagatcc
     3061 ccccgagcgc cggattgcgg agcaagggcg cctcgtaaaa ctgggagaaa tggatatcct
     3121 gcgcccgcgt gcggaaggcc ggcaggagca gggccccggt gagtacgaga attacagcga
     3181 gcgttttcaa ggtatgagcg ttaatgttaa taaggtcata taatcgattt tcacctgatc
     3241 agtgtaacgg tgccgcgcag cgcgatcacc tgcgcattgt tacagatcac ttccacgata
     3301 tacacatagg catcggcggg agcgggcttc ccgttgaagg ttccatccca gcccatggtc
     3361 tccgagttgg ccggaaaatt tttccgttga aacaccattt gtccccagcg gttaaagacg
     3421 gtcatcgatt gaatattata caggctggta ccccggggat aaaaataatc gttgacgcca
     3481 tcgccattcg gcgagaaggt gttcggcacg aaatagtttc cttcgttgca aaccgttttg
     3541 atcaggatcg aagccgtatc ggtgcagttg ttggaatccg tcaccagtac cgaataggtt
     3601 tgatcgaacg tgagtgtcgc tgtggggttg gcgcaatcga tacagctaag gttggccgcg
     3661 ggagtccaga tccaggcgtt tacatcgggg ctgtaggtga agggtatagc aacgggcacg
     3721 ccgaccggcg tagagatctg tttcggtccc ttgatggtcg ggttcggatt gatcgtgacc
     3781 gttccggaag cggtgtcact gcacccgaag gatacatatg ctttcaggtt gaccgtatag
     3841 gtgccggaat tgagcttcac gagcgggttt tgaccggtgg cggtttggcc gttgctgaaa
     3901 ttccagttcc agaagaccgt gtcgacttcg ggaaccacga gtttgccctg gaactgcacg
     3961 aggccctggc aaaggtttcc ggtatcggac acg
//
LOCUS       DATGKE010000288         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1158697, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000288 DATGKE010000000
VERSION     DATGKE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158697"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VKU83_04970"
     CDS             complement(<1..758)
                     /locus_tag="VKU83_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92933.1"
                     /translation="MKFAMVTYRGLPQLTASDRLMISLFAERAIRAEPAVWSDPAVRW
                     QDFAVVLMRSAWDSHLDPQAFDNWLAHLAKIGVHVLNPIPLIRQNQHKFHLRELGEAG
                     VRIIPTVFISRTDRLDLGAVRSRGWKHAVIKPAISANSYFTERFAVDDYAVMEQKYRG
                     IARERELLVQEFMPEIQDFGEISLVFINRKYSHAILKRARQADFRVQAEYGGQAIRFQ
                     AGSEIIETAESILENYAGDILYARVDGVIRGKVF"
     gene            complement(766..>2452)
                     /locus_tag="VKU83_04975"
     CDS             complement(766..>2452)
                     /locus_tag="VKU83_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLI92934.1"
                     /translation="RFGFTQPELDRAKTAYMNQMEESLKEKDKTNSTSYVKEYQEYFL
                     DSVPSPGIAVEYRLTKTELPSIGLADVNGLARKYITDVNRDILVMAPQKEKDALPDSA
                     RMDRWLQDVANERLQPYHEEQSTRPLLSSQPVPGQIRSEQQDTSLHTTTLTLSNGVTV
                     VLKPTNFKNDEILFSAFAPGGTSLYSDSDYQSAANAAELIAANGVGNYDPGQLDQFLE
                     GKQVTVHPYINERFEGVQGRSSRPDLETALQLIYAEFTEPRSDTAISHGIIERIKGSI
                     QNRESDPNLVFRDTVNAILGAYNIRRTAPSIAKLDQVNLMRIYSIYKERFADASNFTF
                     TFVGNLDVNAIKPLLEKYLGGLPADNRHEEARDLGIHIPPGRIEKKVYKGSEPKSTVL
                     LVFSGAFDYSPYNSIQLDALKETLEIRLLQRLREEESGVYNPAVFDNTQKYPRPRYSF
                     IIQFGCSPQNTDKLIASTLDEIDKLKKAGPPQENVDKWRAEEKTVTETQLKTNSFWLN
                     YITDQLQNHDNPDRIRDYNTLLDKVTPDELRQMADRYLSGTNYIQIVLLPENK"
BASE COUNT          458 a          703 c          689 g          602 t
ORIGIN      
        1 taaaaaacct tcccccggat cacgccgtcc accctggcgt acaggatgtc gccggcgtaa
       61 ttttccagga tgctttccgc cgtttcaatg atctccgagc cggcctggaa acgaatggcc
      121 tggccgccat attcggcctg cacccggaaa tccgcttgcc gggcgcgctt taggatcgca
      181 tgcgaatact tgcggttaat gaaaacgagg gatatctcgc cgaagtcttg aatttcgggc
      241 ataaattcct gtacaagcaa ttcccgctcc ctggcgatgc cgcgatactt ctgttccatg
      301 acggcatagt catcgacggc gaaacgttcg gtgaaatagg aattggccga gatggccggt
      361 ttgatgacgg cgtgcttcca ccccctggac cgcactgccc cgaggtcgag ccggtccgtg
      421 cgggagataa agacggtagg gatgatcctg acgccggcct ccccgagttc gcggagatga
      481 aatttgtgct ggttctgcct gatcagtgga ataggattca aaacgtgcac gccgattttc
      541 gccaaatgcg ccagccagtt gtcaaaggct tgcggatcca ggtggctgtc ccatgccgat
      601 ctcatcagga caaccgcgaa gtcctgccat cggaccgcgg gatcgctcca gaccgccggc
      661 tccgcgcgta tcgctctttc ggcaaaaagg gaaatcatca ggcggtcact ggccgtcaac
      721 tgaggcaggc cccggtaagt caccattgca aacttcatcg gctcctcatt tattctccgg
      781 cagcaacacg atctggatat aatttgtccc gctcaggtag cggtcggcca tctgcctgag
      841 ctcgtcgggg gtcaccttat ccagcagagt attataatcc ctgatccgat ccggattgtc
      901 atggttctgt agctgatcgg tgatatagtt gagccaaaag ctgttggtct ttaactgggt
      961 ctccgtaacc gtcttctcct cggcgcgcca tttgtccacg ttttcctgcg gggggccggc
     1021 cttcttcagc ttgtcgatct cgtcgagcgt ggaagcgatc agtttgtccg tattctgagg
     1081 cgaacacccg aactggatga taaaactgta ccgcggccgg ggatattttt gggtgttatc
     1141 aaataccgcc gggttgtaga cgccgctttc ctcctcgcgc agtctttgca gcagccggat
     1201 ctcgagcgtc tccttcagcg catccaattg aatactgttg tacgggctgt agtcgaacgc
     1261 gccggaaaag accagcagca cggtcgactt gggctccgac cccttgtaga ccttcttttc
     1321 gatccggccg ggcgggatat ggatgcccag atcccgggct tcttcgtgcc ggttgtccgc
     1381 gggcaggccg cccaggtatt tctccaataa gggtttgatg gcgttcacat ccagattccc
     1441 tacaaacgtg aaggtgaagt tcgaggcgtc ggcaaaccgc tctttataga tgctgtagat
     1501 ccgcatgagg ttcacctggt ccagcttcgc gatcgagggc gccgttctcc ggatattgta
     1561 cgctcccagg atggcattga cggtgtccct gaagacgaga ttgggatcgc tctcgcggtt
     1621 ttgtatgctc ccctttatcc gctcgatgat tccgtgcgag atggccgtgt ccgacctggg
     1681 ttccgtgaac tctgcgtaga tcaactgcag cgccgtctcc aggtccgggc gcgaggaccg
     1741 cccctgcacc ccttcgaaac gttcgttaat gtagggatgc accgtcacct gcttcccttc
     1801 caggaattga tcgagctggc ccgggtcata attgccgacc ccgttggccg cgatgagctc
     1861 ggctgcattg gccgcgcttt gataatccga atcactgtac agggaggttc cgccgggcgc
     1921 gaaggcgctg aacaggatct cgtcattttt aaaattggtg ggcttcagga ccaccgtcac
     1981 cccgttgctc aaagtaagcg tcgtagtatg cagcgacgtg tcctgctgtt cgctcctgat
     2041 ttgtcccggt accggttgag aggaaagcaa tggtctggtg ctctgctcct cgtggtaggg
     2101 ctgaagcctt tcattggcga cgtcctgtag ccacctgtcc atcctggccg aatccggaag
     2161 ggcatccttt tccttttggg gagccatgac taaaatatcc cggttgacat cggtgatgta
     2221 tttccgcgcc agcccgttga cgtccgccag gccgatcgat ggcagttccg ttttggtcag
     2281 ccggtattcg acggcgatgc ccggcgacgg cacgctgtcc aggaaatatt cctggtattc
     2341 tttcacgtag ctggttgaat tcgtcttatc cttctccttc agggattctt ccatctggtt
     2401 catgtaggcg gtcttcgcgc ggtccagttc cggctgggtg aagccaaatc gc
//
LOCUS       DATGKE010000289         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_355712, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000289 DATGKE010000000
VERSION     DATGKE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355712"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VKU83_04980"
     CDS             <1..1224
                     /locus_tag="VKU83_04980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLI92935.1"
                     /translation="QVESTAVAAGIIAHNFYGQPTEKLQLVGVTGTNGKTTIATLLYK
                     LFTALGYKCGLLSTVQNHVAARVVPATHTTPDAIHLNALLKEMVDADCAYAFMETSSH
                     AIHQQRIAGLRYAGGIFSNITHDHLDYHKTFDEYIRVKKSFFDALPSDAFALSNADDK
                     RGGVMLQNTHAKKYFYSLRTMADFKGKILENNLTGLVMTVNDQEVHFRLIGEFNAYNL
                     LAVYGAAVCLGCNRSEVLRHLSTLGGAEGRFDYLVSRKERIIAIVDYAHTPDALINVL
                     ATIQKLRKGNERIITVVGCGGDRDKTKRPLMGAAACEYSDKVIFTSDNPRSEEPGQII
                     RDIEEGLNTAAKRKYISITDRKEAIRTALNLAAAGDIVLIAGKGHEKYQDIKGVKYPF
                     DDKQIVTEFFELLDK"
     gene            1227..>2178
                     /locus_tag="VKU83_04985"
     CDS             1227..>2178
                     /locus_tag="VKU83_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92936.1"
                     /translation="MLYSLFEWFRQNSIKVPGQALFQFITFRVLLAIVLSLVISMIFG
                     KNLIRYLVKRQIGETVRDLGLAGEQQKKGTPTMGGFIIILAILIPTLLLADLNKAYIR
                     LMLFSTVWLGLIGFIDDYLKLRAKRIAKEQGIPYRKGDKDGLAGWFKVLGQVVLGLVV
                     GATLYFNSNTRVWREYVGTTLPSDTTGLKVVTLGREKKVFVEAREPITTIPFVKNHEF
                     NYSKLLPTAIRGLTWVLYIVIVIFIVTAVSNGANLTDGLDGLATGSSAIIGACLGIFA
                     YASGNIRFAEYLNVMYIPNLGELSIFIGALVGACVGFLWYN"
BASE COUNT          492 a          620 c          606 g          460 t
ORIGIN      
        1 caggtggaaa gtacggccgt ggccgcgggc atcatcgccc acaattttta tggtcagccc
       61 actgaaaagc tgcagctggt cggcgttacc gggaccaatg gcaagacgac gatcgccacc
      121 ctcctgtata agctcttcac cgccctcggc tataaatgcg gcttgctgag tacggtacag
      181 aaccatgtgg ctgcccgtgt cgtccctgcc acccatacca cccccgatgc catccatctc
      241 aatgcgctgc tgaaggaaat ggtggacgcg gactgcgcct atgcgttcat ggagaccagc
      301 tcgcatgcca tccaccagca acggatcgcc ggactgcggt acgctggcgg catattcagc
      361 aatatcacgc acgaccacct ggactatcac aagaccttcg atgaatatat ccgcgtgaaa
      421 aagtcatttt tcgacgccct tccttccgat gcgtttgccc tttccaacgc ggacgataaa
      481 cggggcggcg tgatgttgca gaacacccat gcgaagaaat atttctatag ccttcgcacc
      541 atggccgact tcaaggggaa gatcctggaa aacaacctga ccgggctcgt gatgaccgtc
      601 aatgaccagg aggtgcattt ccgcctcatc ggggaattca acgcgtataa cctgcttgcc
      661 gtctatggcg ccgcggtgtg cctcggctgc aaccggtcgg aagtcttacg ccacctgagc
      721 acgcttggcg gtgcggaagg ccggttcgac tatctcgttt cgcggaagga aaggatcatc
      781 gcgatcgtcg actatgcgca taccccggac gcgctgatca atgtcctggc caccatccag
      841 aagctgcgga agggtaacga acggatcatc accgtagtcg gatgcggcgg cgaccgcgac
      901 aagacgaaac ggccgctgat gggcgccgcc gcctgcgaat atagcgacaa ggtcattttc
      961 acttccgata atccgcgcag cgaagagccc gggcagatca tccgcgacat cgaggagggg
     1021 cttaacacgg cggcaaagcg gaaatatatt tcgatcaccg accgcaaaga ggcgatccgg
     1081 accgcgctta acctggcggc ggccggcgat atcgtgctga tcgcaggcaa gggccacgaa
     1141 aagtatcagg atataaaggg cgtgaaatat ccatttgatg ataagcagat cgtaaccgaa
     1201 tttttcgaac tgttagataa ataagcatgc tttattcttt atttgaatgg ttcagacaaa
     1261 acagcatcaa ggtgccgggt caggcccttt tccaatttat cacgttccgc gtactgcttg
     1321 ccatcgtcct gtccctagtc atcagtatga tcttcggaaa gaacctgatc cgttacctgg
     1381 taaaacgtca gatcggcgaa accgtccggg acctggggct ggccggcgaa cagcagaaga
     1441 agggcactcc cacgatgggc ggattcatca tcattctcgc gatcctcatt ccgacgctcc
     1501 tgctggccga tcttaacaag gcttatatcc gcctgatgct cttcagcacc gtctggcttg
     1561 gcctgatcgg ctttatcgac gactatctca agctgcgggc caagcggatc gcaaaggaac
     1621 agggcattcc ctaccggaag ggcgacaagg acggtctcgc gggctggttc aaggtcctgg
     1681 gccaggttgt gctgggcctc gtagtgggcg caacgcttta tttcaacagc aatacacggg
     1741 tctggcggga atacgtggga acgacgcttc cttcggatac gaccggcctg aaagtggtaa
     1801 cgctggggcg ggagaagaaa gtctttgtgg aggcccgcga gccgatcacg acgatcccct
     1861 ttgtcaaaaa ccacgagttc aattattcga agctgcttcc tactgccatc cgcggactga
     1921 cctgggtact ctatatcgtc atcgtcatat ttatcgtcac ggcggtatcc aacggcgcga
     1981 accttacgga cgggctggac ggcctggcga ccggctcaag cgccatcatt ggcgcctgcc
     2041 tgggcatttt cgcgtacgcg agcggcaata tccggtttgc cgaatacctg aatgtgatgt
     2101 atatccccaa cctcggcgag ctgtcgatct tcatcggcgc gctggtaggg gcctgcgtgg
     2161 gatttctttg gtacaatg
//
LOCUS       DATGKE010000290         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_742032, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000290 DATGKE010000000
VERSION     DATGKE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742032"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="metX"
                     /locus_tag="VKU83_04990"
     CDS             complement(<1..992)
                     /gene="metX"
                     /locus_tag="VKU83_04990"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HLI92937.1"
                     /translation="MNYFDYDQPFELEGGAALPGIRIAYHVYGKLNADGSNVVWVCHA
                     LTANSAVAEWWPGVVGDRGAIRPEEYFIVCANILGSCYGSSGPLTGDPLTGEPYYERF
                     PVVTIRDMVRAHILLRKHLGIDRIHLLMGGSMGGYQVLEWAVMEQDVIGKLFLIATSP
                     AESAWGIAIHTAQRLAIEADPTWNAPMPDAGRRGLKAARAIGILTYRNYGIMVKRQTD
                     PDCEKLDHYRASSYIQYQGDKLISRFHAYSYWLLTKAMDSHQLARGRGGRLERVLGEI
                     RQKVLLVGLTSDILCPVEEQLFMAAHLPDADMVEIDSAYGHDGFMVEGEKIATL"
     gene            1062..1484
                     /locus_tag="VKU83_04995"
     CDS             1062..1484
                     /locus_tag="VKU83_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLI92938.1"
                     /translation="MVRIELSRVEGDFGFEARDAYGHTARMDSNPETGGAGFGIRPMQ
                     MLLMGLGGCSGIDIVSILKKQRQTVEGFRMIIDGDREKGNDANLWEKVHLVFELKGQI
                     DGDKARKACELSMDKYCSVATTLRKAGCHITWEVRITS"
     gene            1503..>2127
                     /locus_tag="VKU83_05000"
     CDS             1503..>2127
                     /locus_tag="VKU83_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLI92939.1"
                     /translation="MSLQRITQAIRIRASRTPEKEHSSPLYLTSSFCYDDAEEMRAVF
                     ADEKEAFIYSRFSNPNVQEFTDRLCALEGAEAGFATASGMSAIVASFLSLLKKGDHLV
                     SCHSIFGSTHTVITKYLPRYGIDYTYVGTADPASWEAAIRPNTRMIYLETPTNPGLDI
                     IDLEAAGAVARKHHLILNVDNCFATPVGQTPIEYGADLVIHSATKWLD"
BASE COUNT          496 a          637 c          553 g          441 t
ORIGIN      
        1 aacagagtgg ctattttttc gccttcgacc ataaagccat cgtgtccata ggctgaatcg
       61 atctcgacca tgtcggcatc cggcagatgt gccgccataa aaagctgctc ctctacaggg
      121 cataggatgt cgctcgtcag tccgaccaat aaaaccttct gccggatctc ccccagcacc
      181 cgctccaacc ttccaccgcg ccccctcgcc agttgatggc tgtccatcgc tttggtcagc
      241 agccaatagg aataggcatg gaaccggctg atcagcttgt ccccctggta ttggatataa
      301 gatgaggccc ggtaatgatc gagcttttcg caatcggggt ccgtttgccg tttaaccatt
      361 atgccataat tcctgtaggt gaggatgcct atcgcccgtg ctgctttcaa cccgcggcgg
      421 cccgcgtcgg gcatcggggc gttccaggtc gggtcggcct cgatcgccag tcgctgtgca
      481 gtgtggatcg caatgcccca ggcactttca gcgggcgagg tggcaatcag gaaaagcttg
      541 ccgatcacgt cctgctccat gactgcccat tcgagtacct gatagccgcc catgctgccg
      601 cccatcagaa gatggatccg gtcaatgcca aggtgcttcc gcaataagat atgcgcccgg
      661 accatatcgc ggatggtcac gacaggaaaa cgttcgtaat agggctcgcc ggtcagagga
      721 tccccggtca ggggaccgga gctaccgtaa caggagccaa ggatattggc gcaaacgatg
      781 aaatactctt caggccggat cgctcccctg tcgccgacca cccccggcca ccactcggca
      841 acggctgaat tcgccgtcaa ggcatgacag acccaaacga cattactccc gtccgcattt
      901 aatttgccat acacatgata agcgatcctg atccccggca aagcagcgcc cccctccagt
      961 tcgaaaggct gatcgtagtc gaaataattc atggagccaa ttcttctatt taggtgggaa
     1021 attactgttt aggtgctgta aatttgcaaa aatgtttgat tatggtccgg attgaattaa
     1081 gcagggtcga aggcgatttc ggatttgaag ccagggacgc ctacggccac accgcccgaa
     1141 tggatagcaa tccggagacc ggcggcgccg gcttcggcat ccgcccgatg caaatgctgc
     1201 tcatgggatt gggcggatgc agcggcatcg atatcgtatc cattctcaaa aagcaacggc
     1261 aaaccgttga aggtttccgg atgatcatcg acggcgaccg ggaaaaggga aacgatgcca
     1321 acctctggga gaaggtccac ctggtttttg aactgaaagg ccagatcgac ggggacaagg
     1381 caagaaaagc ctgcgagctc tccatggaca aatattgctc ggtcgccact acgctccgga
     1441 aagccggttg ccatatcacc tgggaggttc gcatcaccag ttaacaccaa aatttcctat
     1501 ccatgtcttt acaacgcatc acgcaggcca tccgcatccg tgccagccgt actccggaga
     1561 aagaacattc ctcgccatta tatctcacca gcagcttctg ttatgacgac gccgaggaaa
     1621 tgcgcgccgt atttgccgac gaaaaagaag cgtttattta tagccgtttc agcaatccga
     1681 atgtccagga attcaccgac cgcctgtgcg cgctggaagg agctgaagcg ggtttcgcca
     1741 cagcctcagg catgagcgcc atcgtcgcgt ccttccttag cctcctcaaa aaaggcgatc
     1801 acctcgtctc gtgccattcg atttttggca gtacgcatac cgtcattacc aaatacctgc
     1861 cgagatacgg catcgattat acctatgtgg gtaccgccga cccggcatcc tgggaagcgg
     1921 ccatccggcc gaacacgcgg atgatctacc tcgaaacgcc caccaacccg ggcctcgaca
     1981 tcatcgacct cgaggctgcc ggggccgtag cgcggaagca ccacctgatc ctcaacgtgg
     2041 acaactgttt tgcgacgccg gttggccaaa ccccaatcga gtatggcgcg gacctcgtca
     2101 tccattccgc cacgaagtgg ctcgacg
//
LOCUS       DATGKE010000291         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1065917, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000291 DATGKE010000000
VERSION     DATGKE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065917"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2954
                     /locus_tag="VKU83_05005"
     CDS             <1..2954
                     /locus_tag="VKU83_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI92940.1"
                     /translation="NRVGIAQPLLPSVVSKLGLTVRASNPDQLMLVAVYSPKGTHNIT
                     FLDNYTDIFIQEAILRVPGVGDIQAWGDQFAMRIWMNPQKMASYHITPTDVQSALNSQ
                     NVYVAAGAVGAPPQASAQAHEYSIVVNGILNRAADYDNIVIKTNPGTGQMVYLKDIAR
                     VELGKFTFSSNDFVDGKHCSFLMIYQTPGSNALKTADGVYAKLAELKKTFPTDVDYAV
                     PFEAVTIIRVSMGEVVKTLLKALALVAFVVFLFLQSWRATLIPIFAIPVSLLGAFCFF
                     IPLGFTINTLTMFGFVLAIGIVVDDAIIVVEAVQHYIDDRHMSAKEATYRAMKEISAP
                     VVAIALILAAVFVPVGFIPGIVGRLYQQFAITIAISVILSAFVALSLTPALCTLLLKP
                     HDPQRKKNWLDRGFGWFNKGFDRFITGYTHGVRRSIRYSWLIVLLLLGMCTGAFFLFK
                     TKPSGFVPMEDGGRLFIGFQLADANSTTETVNVMEKIIAVVNSTPGILHYSAFSGFNI
                     LSHGNTSNGGSFFIMLKPWDERTAPETQWPGIKNVIDRKVAAAGIKNANVVVIQPPPI
                     RGIGIAAGFAIQIEEGNSTDDVYTFEKTVQRFVAEAQKNPSTSTAFCYFSAHTPSYDL
                     SVDRVKCEKLGVDISQVFSTMQAFMGSLFTNNFTLYNRTYHVVIQADTDYRAMIGDMN
                     KYYVRNRSDSMVPLSTLISYKASVAPPLITHFNIFRSAEVDGSIPAGYSSGQSIADLR
                     KLAARYLPRGYTYDFSGLSYEEIKAGSMTVYIFTFCIVFVFLFLAALYESWSVPLAVM
                     LAVPISAFGAIVALTIAPTLTDNVYAQVGLITLIGLSAKNAILIVEFAKIRVDHGEDL
                     IKSTLRAAELRIRPIIMTSLSFIFGVMPLVLASGAGGASRNTIGVTVLGGMIASSTIS
                     IFIVPVLYVLLTRWSYGKKELAWLRAHREELQEKARRVEERNIDPALEYEIRQAHLEH
                     REFRARGGGGE"
     gene            complement(2973..>3676)
                     /locus_tag="VKU83_05010"
     CDS             complement(2973..>3676)
                     /locus_tag="VKU83_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI92941.1"
                     /translation="ELLKDSPKRPMSWLIGRHFGFDSAGYIVGIAKNFNFNSLHAKIE
                     TMFLFNQTQWGFRTVSARIDGARANAALDFIQSTWKSIFPDHPFEYQFLDDHFRDVYR
                     ADRQVSTMVTILAILAIFISCLGLFGLTSYSAEKRIKEIGIRKVMGASVQSIVTLLSR
                     QFMGLVLWANLLALPLAGWAIYEWLQDYAYRINLSWWVFALAVLLAFAVALITISVLA
                     FRAAVANPVKSLRAE"
BASE COUNT          808 a          974 c         1055 g          839 t
ORIGIN      
        1 agaaccgggt gggtattgcc cagcccctgc ttccttccgt ggtcagcaag ctggggctga
       61 cggtgcgggc cagcaatccg gaccagttga tgctcgtagc cgtgtattcg ccgaagggaa
      121 cccacaacat cacctttctg gacaactata cggacatctt catccaggag gccattttac
      181 gggtcccggg tgtgggagat attcaggcct ggggggacca gttcgccatg cggatctgga
      241 tgaatccgca aaaaatggcc tcataccata tcacgcctac cgatgtgcaa agcgcgctga
      301 attcgcaaaa tgtatatgta gcggcaggcg ccgtgggcgc tccgccacag gcatcggcgc
      361 aggcacacga gtacagtatc gtcgtcaacg gaatcctgaa ccgggcggcc gattacgaca
      421 acatcgtgat caagaccaac ccgggtacgg ggcaaatggt ctacctgaag gacattgcgc
      481 gcgtggagct ggggaaattt accttttcca gcaatgactt tgttgacggg aagcattgct
      541 cgtttctgat gatctatcaa actcccggca gcaatgccct aaagacagca gacggcgttt
      601 atgccaaact cgccgagctg aagaaaacct ttcccacaga cgttgattat gcggtgccat
      661 tcgaggctgt tacgatcatc cgggtttcga tgggggaagt ggtgaagacg ttgttgaagg
      721 cgctggccct ggttgccttt gtggtgttcc tgttcctgca gagttggcgg gcaacgctta
      781 ttccgatctt tgcgatcccc gtgtctctcc tgggggcctt ttgctttttt attccgcttg
      841 gctttacgat caacacgctg acaatgttcg gattcgtcct cgccatcggg atcgtggttg
      901 acgatgctat catcgtggta gaagcggttc agcattatat cgatgaccgg catatgtctg
      961 cgaaggaagc gacataccgc gcgatgaaag agatctcggc gccggtcgtt gcgatcgcgc
     1021 tgatcctggc ggcagtgttc gttccggtgg gttttattcc cgggattgtt ggacggctgt
     1081 atcagcaatt tgcgatcacg atcgccattt cagtaatctt gtctgcgttc gttgccttgt
     1141 ccctgacgcc ggccctttgc acgctattat tgaagccaca cgatccccaa aggaaaaaaa
     1201 attggctgga ccgggggttc ggctggttca acaaaggatt tgaccggttc atcaccggct
     1261 atacccatgg ggtcaggcgg agcatacgct attcgtggct cattgtcctg ctcctgctgg
     1321 gaatgtgcac gggcgcattt ttccttttca agacgaaacc atccggattt gtgccgatgg
     1381 aagacggagg acggctgttc attggctttc aattggccga tgcgaactca accacggaaa
     1441 cggttaacgt gatggaaaag atcatagcgg tcgtgaattc gacgcccggc atccttcact
     1501 actctgcgtt ttccggattc aacatcctca gtcatggcaa cacgtcgaac gggggctcct
     1561 tttttatcat gctgaaaccc tgggacgagc gcacggcacc cgagacgcag tggcccggta
     1621 tcaagaacgt aatcgaccgg aaggtcgctg ccgccgggat caagaacgcc aacgtcgttg
     1681 tcatccagcc accgccgatc cgggggatcg gaattgcggc gggatttgcc attcaaattg
     1741 aagaaggcaa ctcgacggat gacgtctata cgttcgaaaa aacagtacaa cggttcgtag
     1801 cggaggcaca gaaaaatcca tccacgtcga ccgccttctg ttacttttct gcccatacac
     1861 catcttatga tctgtcggtc gaccgggtga agtgcgagaa gctgggcgta gatatctccc
     1921 aggttttctc gacgatgcag gcctttatgg gcagcctgtt caccaataac ttcacgctat
     1981 acaaccggac ctatcacgtc gtcattcagg ccgacacgga ttatcgggcg atgatcgggg
     2041 acatgaacaa gtactatgtg cgcaaccggt cggattcgat ggtgcctctg agcacgctca
     2101 tcagttacaa agcctcggtt gcgccgccac tgatcaccca ttttaatatt tttcggtcgg
     2161 cggaagtgga cggctcgatc ccggcggggt acagcagcgg gcaaagcatt gctgatctaa
     2221 ggaagctggc cgcaaggtat ttgccacgag gatataccta cgatttctcc ggacttagtt
     2281 acgaggagat caaggcgggc tcaatgacag tatatatttt taccttttgt attgtctttg
     2341 tcttcctgtt tctggcagcc ctttacgaga gctggtccgt tccactggct gttatgctgg
     2401 ccgttcccat cagcgccttt ggcgcgatcg tggccctgac cattgcaccg acactcacgg
     2461 ataacgttta cgcgcaggtg ggcttgatca ccctgatcgg tctgtccgca aaaaacgcga
     2521 tcctgatcgt ggaatttgca aagatcaggg tcgaccatgg tgaggatctt atcaaatcga
     2581 cgctgcgggc ggcggagctt cggatccggc cgatcattat gacttcgctt tcattcattt
     2641 ttggtgtgat gccgctcgta cttgcctccg gcgccggcgg cgcgtcgagg aacacgatcg
     2701 gcgtaaccgt gctgggcggc atgatcgcct cttcgaccat ttcgatcttc atcgtgccgg
     2761 tattgtatgt cctgctcacc cgttggtctt atgggaaaaa agagctggca tggttgcggg
     2821 cgcaccgcga agaattgcag gaaaaggcaa ggcgggtgga ggagcgcaat atcgatccgg
     2881 cgctggagta cgagatccgg caggcgcacc tggaacatcg ggaattcagg gcccggggtg
     2941 gcggtgggga gtgatccgcc ggcctgcacg ctctattcag ccctcaagct tttaaccggg
     3001 ttggcgacgg cggcgcggaa ggccaggacg ctaatcgtga tgagggcgac ggcgaatgcc
     3061 agaagaaccg ccagcgcaaa cacccaccag ctcaaattga tccggtaggc gtagtcctga
     3121 agccactcat aaatggccca accggccagg ggcagggcaa gcaggttcgc ccagagtacc
     3181 agacccatga attgccggga aagcaacgtt acaatgctct gcaccgaggc gcccattacc
     3241 ttgcggattc cgatctcctt gatccgtttt tccgccgaat aactcgtcag gccgaagagg
     3301 cccaggcagg agataaaaat cgcgaggatg gccaggatgg tcaccatggt ggagacctgc
     3361 cggtcggcgc gatagacgtc gcggaaatga tcatcgagga actggtattc gaacgggtgg
     3421 tcgggaaata tgcttttcca ggtggattgg ataaaatcga gcgcggcatt cgcgcgggcg
     3481 ccgtcgatcc gtgcggagac ggtacggaag ccccattgcg tctggttgaa caggaacatc
     3541 gtttcgatct tcgcgtggag agaattaaaa ttgaaatttt tagcaatccc cacaatataa
     3601 ccggcggagt caaatccaaa atgcctcccg atcagccagg acatcgggcg cttgggcgag
     3661 tccttcaaca gctcct
//
LOCUS       DATGKE010000292         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1521299, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000292 DATGKE010000000
VERSION     DATGKE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521299"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..531)
                     /locus_tag="VKU83_05015"
     CDS             complement(229..531)
                     /locus_tag="VKU83_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HLI92942.1"
                     /translation="MTVYNITCKVRWEILEGWLAWQREEQVPATLATGFFDEYRFFRL
                     LEQEEEEGPTFVIQFLTSSLERYQRFLMESEPGLRRAGWEKWGSGYIAFRTLMESV"
     gene            complement(535..1317)
                     /locus_tag="VKU83_05020"
     CDS             complement(535..1317)
                     /locus_tag="VKU83_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLI92943.1"
                     /translation="MPSIRIVSYNVNGIRAAMNKGLVEWLRTDPADIICIQETKAHRE
                     NVAHERITGLGYHDYWFSARKKGYSGVAVFTKLKPDHVEYGTGHAVSDDEGRVIQLDF
                     GGIRLINAYFPSGTSGDERQTYKYQWLDEFYEYVENLRRRIPRLIVCGDYNIAHREID
                     IHDPKGNKNNSGFLPAERAWMDKFLTSGWVDGFRLLNPDPHHYSWWSQRFPSVRLQNK
                     GWRIDYISVTDALKGNLIKAEIYPEVKHSDHCPVYLELNLSD"
     gene            1355..2101
                     /locus_tag="VKU83_05025"
     CDS             1355..2101
                     /locus_tag="VKU83_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLI92944.1"
                     /translation="MDKFDRIIPAKESQFLEGPKSRAYELRFAWRVFREFIKGFRTLH
                     FIGPCITVFGSARFKEDHPHYQSAREMGRQIAAMGFTTMTGGGPGTMEAANRGAFEQG
                     GMSVGCNIKLPFEQRPNPYVQTSITFEHFFVRKVMLVKYSYAFVIMPGGFGTMDEFFE
                     TLTLVQTKILIRFPLVLFGKEYYEPLFAYMQEMAEQGTISTEDLALVLLTDSIAEAIQ
                     HIDTFIKKTYRRRPARRKWWLFERPISDVK"
     gene            complement(2085..2909)
                     /locus_tag="VKU83_05030"
     CDS             complement(2085..2909)
                     /locus_tag="VKU83_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92945.1"
                     /translation="MAYFLAGRHVARVLLISVVPFAASAQQTPPKKKKHFVKEVYGSW
                     GYNGEFYTNSTIHVSQSALGNDYNFGNVRAHDHPGWNEGLFSKALSIPQYNYRLGFLL
                     NEEKGWGFEINFDHTKYIFADQNVTVRGKLNFRNVDTTVAFNAANGFYYYLNNGANFL
                     LFNVTKKWHLLTRANGRFKLDLLGKFGVGPVIPHTQNELFGQKNDEGFQFGGWNTGAE
                     ASLRATFCRYVYLEFSNKLDYARYHNLNVYMGAAHQAFGTEEMILSLGFVLPTSHH"
     gene            3041..3469
                     /locus_tag="VKU83_05035"
     CDS             3041..3469
                     /locus_tag="VKU83_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92946.1"
                     /translation="MTTERLDTHPEKDPRAGDDARLLRKQIGSTRTFLYIAAALNFIS
                     ALLLTPNLPSPATVVNITVTCLIGGLYILLAFRAGKKPYTAIRLGLLLLVLVVLADIL
                     VSPIGPFNRWQSKLLTLVMLLMGLADSRDAQRKMSKPKTA"
BASE COUNT          835 a         1007 c          904 g          838 t
ORIGIN      
        1 atacccgctt tttgcatccg acaaaatttt tttaacctgt aagacacaac gtcgatgctg
       61 gccgaatccg ctccggacaa ggattcccgg cgaaacgcga tcgccggttt gcggttgcaa
      121 tcaaggcaca atcccctgaa aggcaggccc gtaaagtatt gcagcaggca tggcctgaaa
      181 gccttgccag tcaaggctct tgcccacaaa tggtgcacag gatcttacct aaaccgactc
      241 catcagcgtg cgaaatgcaa tgtacccgct gccccatttt tcccatcctg cacgcctcag
      301 gccgggctcg gactccatga gaaagcgttg atatctttcc agggaactgg tgagaaactg
      361 gatgacaaat gttggcccct cctcttcttc ctgttccaat aaccggaaaa accggtattc
      421 atcgaagaac ccggtcgcaa gggtggcagg cacctgctct tcgcgctgcc aggccagcca
      481 gccttcaagg atctcccaac gcaccttaca agtaatatta tagacggtca ttcttcaatc
      541 gctcaggttc aattccagat aaaccggaca atgatcgctg tgcttcacct ccggatagat
      601 ctccgctttg atcaagttgc ccttcagggc atccgttaca ctaatgtaat cgatccgcca
      661 gcccttgttc tgcaacctga cggacggaaa acgctggctc caccacgaat aatgatgagg
      721 gtccgggttg agcagccgga aaccatccac ccatccgctg gtaagaaatt tatccatcca
      781 ggcccgctcg gcgggaagaa agcccgagtt attcttattt cccttcggat catgaatatc
      841 gatctcccgg tgcgcgatat tgtagtcgcc gcacacgatc agccggggaa tgcgccgccg
      901 taaattctca acgtattcat agaattcatc cagccactga tacttatagg tttggcgttc
      961 gtcgccggaa gtcccggagg ggaaatacgc gttgatcagc cggatcccgc caaagtccag
     1021 ttggatgacc cgcccctcat cgtcgctgac tgcatgcccc gttccatatt caacatggtc
     1081 cggtttcagc ttcgtaaaga cagcgacacc gctataacct ttttttcggg ccgaaaacca
     1141 gtagtcatga tagcccaggc cggttatgcg ttcgtgggcc acattttccc ggtgcgcctt
     1201 ggtctcctgg atgcaaatga tatctgcggg gtccgtcctc agccattcca ccaggccttt
     1261 gttcatcgct gcccggatgc cgttaacgtt atagctaaca atgcgaattg aaggcataat
     1321 cggctatatt agcggcgaaa tatacgcaat atctatggat aagttcgaca ggatcattcc
     1381 cgcaaaggag agccagttcc tggaaggccc gaagagccgc gcctatgagc tgcgttttgc
     1441 ctggcgggtg ttcagggaat tcatcaaggg cttccgtacg ctccatttca ttggcccttg
     1501 tattacggtt tttggttccg ctcgtttcaa ggaagaccac ccgcactatc aatccgcgcg
     1561 ggagatgggc cgccagattg cagcgatggg cttcacaacg atgacgggcg ggggcccggg
     1621 aaccatggaa gccgccaacc gcggcgcatt tgaacaggga ggcatgtccg ttggctgtaa
     1681 catcaagctg cccttcgaac agagaccgaa tccctatgtc cagacatcca tcaccttcga
     1741 gcatttcttc gtccggaagg tgatgctggt gaagtactct tatgccttcg tcatcatgcc
     1801 cggagggttc ggcacgatgg atgaattttt cgagaccctg acgctcgtgc agacaaagat
     1861 cctgatccgc tttccgctcg tccttttcgg gaaggaatat tatgagcctt tatttgccta
     1921 tatgcaggaa atggcggaac aaggcactat ttcgacggag gacctggccc tggtgttgct
     1981 cacggacagc attgccgagg cgatacagca tatcgacacg ttcataaaga agacctaccg
     2041 gcgccggccg gctcgccgga aatggtggct cttcgagcgt cccatcagtg atgtgaagta
     2101 ggcaaaacaa aaccgaggct caggatcatc tcctcggtac cgaatgcctg gtgcgcggca
     2161 cccatgtaga cattcaggtt gtggtaccgc gcatagtcca gcttgttaga gaactccagg
     2221 tagacgtacc tgcagaaagt ggccctcagc gacgcctcgg ccccggtatt ccagccgcca
     2281 aattgaaagc cttcatcatt cttctggccg aaaagctcat tttgggtgtg cggaatcacc
     2341 ggccctacgc cgaacttgcc cagtaagtcg agtttgaacc tgccgttcgc ccgggtgagg
     2401 agatgccatt ttttggtaac attgaataac agaaaattgg cgccattatt caggtagtaa
     2461 tagaacccgt tggcggcatt gaaggcgacg gttgtatcta cattccgaaa gttgagcttg
     2521 cctctcacag taacgttctg gtcggcaaag atgtatttcg tgtgatcgaa gttgatctca
     2581 aatccccagc ctttttcctc attgagcaga aatcccagcc gataattgta ttgcgggatc
     2641 gacaaagcct tgctgaagag gccttcgttc cagcccggat ggtcatgcgc cctgacgttg
     2701 ccaaagttgt agtcgttgcc cagcgcggat tgcgagacat ggatcgtgct attcgtgtaa
     2761 aactcgccat tgtagcccca ggagccatag acctccttga caaaatgttt cttcttctta
     2821 gggggggtct gctgggcaga agccgcgaag ggaacaacgg atatcagcaa caccctggcc
     2881 acatgtcgac ccgccaagaa ataggccatt aatctcatat ctttctcaag cttttaatag
     2941 aatgatagcg gacaaaagta tagaaatatt gttatcttat tagcttaatc acgaaattgc
     3001 gtggaatctg ccaggaccat gaacttcaac cacaagccga gtgacaaccg aacgtcttga
     3061 cacccatcct gaaaaagatc cccgcgccgg ggatgatgca cgcctgctgc ggaagcagat
     3121 cgggtcgacc cgcacttttc tgtacatcgc cgccgcgctg aacttcattt ctgcactgct
     3181 cctgacgccg aacctgccgt caccggcgac tgtagtcaat ataacggtca cctgccttat
     3241 cggggggctg tatattctgc tggcattccg ggcgggcaag aagccctata cggcgatccg
     3301 gctgggcctc ctgctcctcg tgctggtggt cctcgcggac atcctagtga gcccgattgg
     3361 tcctttcaac cgctggcagt cgaaattgct caccctggtg atgctattga tgggattggc
     3421 cgacagccgc gatgcccaaa gaaaaatgtc aaagccgaag acggcatgaa tcaggcatca
     3481 tattccagca cggggcgcag ccatctttcc gcttcctcca gcggcatgcc cttcctggcc
     3541 gcatagtcca ggacctgatc cttttcaatc tttccgacgc cgaa
//
LOCUS       DATGKE010000293         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_338136, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000293 DATGKE010000000
VERSION     DATGKE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338136"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VKU83_05040"
     CDS             complement(<1..545)
                     /locus_tag="VKU83_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92947.1"
                     /translation="MKIRLRSLLFISLVLGLGCCSCKKRVDYPANPLTNYFLDLQVGK
                     YVTYRMDSLNFYYYGQNDTVTSYLAKDSVEKKTVDNTGATAWLVTRYLSDTTGSSWVP
                     SITYVVSPSAQAIDVTENNLRFVKLAFPMDLGFSWTGNTYLPYNPYQDFFVYSDYNNL
                     TMANWNYYYSQVNQPFTVANGT"
     gene            complement(591..1688)
                     /gene="mnmA"
                     /locus_tag="VKU83_05045"
     CDS             complement(591..1688)
                     /gene="mnmA"
                     /locus_tag="VKU83_05045"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLI92948.1"
                     /translation="MSRNGKVLVAMSGGIDSTVTALMLHDQGYEVVGITMKTWDYASA
                     GSAKKETGCCNLDSFNDARMAAVQHGFAHFVLDIREQFGDFVIENFVDEYLAGRTPNP
                     CVLCNTHIKWRALLKRADALDCAFIATGHYAMVRQAATGRYSIAKGVDETKDQSYVLW
                     GLQQDLLTRTLLPLGPYRKTAIRQLAMDYGYPELARKSESYEICFVPDNDYRGFLRRR
                     VEGLDQRVAGGNFVDKEGNILGKHRGYPFYTIGQRKGLDITFGKPVYVTAIRPETNTI
                     VLGDECDLDRPGMRVAKMNWMKYEAIAPGMEAVTKVRYKDPGTLSRLYPDGDSAVKVS
                     FCEMAKSIAPGQSAVFYEGDEVIGGGIIQRG"
     gene            1849..>2204
                     /locus_tag="VKU83_05050"
     CDS             1849..>2204
                     /locus_tag="VKU83_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLI92949.1"
                     /translation="MIKLQVIGNLGKDCVTNTVNGKNVINFNVAHTEKFRDSQGNQKD
                     KTIWVECAYWTDRTGVAPYLKKGTQVYVEGTPEVRTYPKNDGTTGVSLSLRVQSVQLL
                     GSRGSEAGGGYSGGGGA"
BASE COUNT          513 a          636 c          572 g          483 t
ORIGIN      
        1 gtcccgttcg ctaccgtgaa aggctggttg acctgcgaat aataataatt ccagttcgcc
       61 atggtcaggt tgttgtaatc gctgtacaca aagaaatcct ggtaggggtt atacggcaag
      121 taagtattgc cggtccagct aaatcccagg tccatcggga aggcaagttt gacaaaccgg
      181 agattgtttt ccgttacatc gatcgcctgg gcgctggggg aaacgacata ggttatcgat
      241 ggtacccagg aagacccggt ggtatcgctc aaataccggg tcacaagcca agcggtcgct
      301 cccgtattgt ccaccgtctt tttttcgacc gaatccttcg ccaggtagct ggtgacggta
      361 tcgttctggc cgtagtaata aaaattgagt gaatccatcc ggtaagtgac atatttaccg
      421 acctgtaagt caaggaaata atttgtgagc ggattcgcgg gataatcgac ccttttcttg
      481 cagctgcagc aacccagccc gagcacgagc gaaataaaga ggagggaacg cagcctgatt
      541 ttcatgtagg actaacgctt aactcactga tttattgcca ttccggggtc tcagccgcgc
      601 tgaataattc cacccccgat gacttcatcc ccttcgtaga atacggcgct ctggcccggc
      661 gcgatgcttt tcgccatttc gcagaaacta accttgaccg ccgagtcgcc atcgggataa
      721 agccggctca gcgtcccggg atccttatag cggaccttgg tcaccgcctc cattccaggg
      781 gctatcgcct catacttcat ccagttcatc tttgccacgc gcatcccggg gcggtcgagg
      841 tcgcattcat ctcccagaac gatggtattg gtctccggcc ggatggccgt gacgtacacc
      901 ggcttgccaa aggttatgtc caagccctta cgctgtccga tggtataaaa aggataaccc
      961 cggtgctttc cgaggatatt cccttcctta tcgacaaaat tccccccggc cacgcgctgg
     1021 tcgagcccct cgacccggcg ccgcaggaac ccgcgatagt cattgtcggg aacaaaacag
     1081 atttcatagc tttcgctctt ccttgccagc tccggataac catagtccat cgccagctgc
     1141 cggatcgccg tcttccggta aggaccgagc ggcaacagtg tcctggtcag cagatcctgc
     1201 tgcaagcccc acaaaacata gctctggtcc ttggtttcgt cgaccccctt cgcgatgctg
     1261 tagcggccgg tagcggcctg cctcaccatg gcgtaatgac ccgtggcgat gaacgcgcaa
     1321 tccagggcat ccgcgcgctt cagcaaagcc cgccatttga tgtgggtgtt gcatagcaca
     1381 cagggattcg gcgtccttcc cgcgaggtat tcgtcaacaa agttttcaat cacgaagtcg
     1441 ccgaactgct cccggatatc cagcacaaaa tgggcaaacc cgtgctgcac cgcggccatc
     1501 cgcgcgtcat tgaagctgtc caggttgcag cagccggttt ccttttttgc ggagcccgcg
     1561 cttgcatagt cccaggtctt catggtgatt cccacgacct catacccctg gtcatgcagc
     1621 atcagtgcag taacggtact gtcaataccg ccgctcatcg ccaccaaaac cttcccattc
     1681 cggctcatag ctgaattttt accaaggggc aaatgtacgc caaaaaccct taccgggcaa
     1741 acgggccggc tgcgtcagcc ggcaacaccg tgcgtaaaat cgtgaaaact tctcccgccc
     1801 gccgggttta aaaactgtaa attgccggta actgtaaaaa ttaccattat gattaagctt
     1861 caagtaatcg gaaacctggg caaggattgt gtcaccaaca cagtgaacgg aaaaaatgtc
     1921 atcaatttca acgtggccca cacggagaaa ttccgggact cccagggtaa ccagaaggac
     1981 aagacgatct gggtggaatg tgcatactgg accgatcgta cgggagtagc gccgtatctc
     2041 aaaaaaggca cccaggtata cgtggaaggg actccggagg ttaggaccta tccgaagaac
     2101 gacggcacaa ccggcgtctc cctaagtctc cgggtccaga gcgtccagct tttgggcagc
     2161 cggggatcgg aagccggtgg cggctattcg ggcggcggcg gagc
//
LOCUS       DATGKE010000294         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_949674, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000294 DATGKE010000000
VERSION     DATGKE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949674"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VKU83_05055"
     CDS             complement(<1..1118)
                     /locus_tag="VKU83_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI92950.1"
                     /translation="MKNTVLIFCCLLLASAALSQAPLRHTAWLKRSNIYEVNIRQYTR
                     EGTFSAFQPNLDRLRAMGVDILWIMPINPISKVDRKGTLGSYYAVADYTAVNPEFGTL
                     NDFRELVRAAHAKGMKVILDWVPNHTGADNRWLTEHPDFYKKDSTGKAAVPFDWTDTR
                     QLDYGNPELRDSMIASMQYWIRETNIDGFRCDVAWNVPGDFWRTCIARLRKMKSLFML
                     AEGDKPYLAENGFDALYPWDYFHTMIRVAKGELPAQSLDSVMEREQQTYPKGTLEMYF
                     TSNHDENSWNGADYNTFPGAAHAPFAVLTQTLPENIPLIYSGQEEPVLRAISFFEKDS
                     IRFRHFDRSPFYKTLLDLRKRDPALDADAAFTKLSVGD"
     gene            complement(1206..>2093)
                     /locus_tag="VKU83_05060"
     CDS             complement(1206..>2093)
                     /locus_tag="VKU83_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HLI92951.1"
                     /translation="IRTWLDKYGMRCSGEIDITRTRWSEQPTMLLPLILSNIKNLDAG
                     AGHRKFEAGRQQALTKTAELLHRLKLLPDGDRKAEETGQTIALLRNTIGFREYPKYRI
                     INHLFAYKQALLNEANHLVRNKIIHKKEDIYYLTFEELAEVVRTGRLDHHLIAARKAE
                     HATFEKLTPPRVITSDGEIITGSYDRENLPPGAFPGLPVSSGIVEGRARVILRMEDAD
                     LAAGDILITTFTDPSWTPLFVYIKGLVTEVGGLMTHGAVIAREYGLPAVVGVNGATRL
                     IRDGQRVRLNGAEGYVQIL"
BASE COUNT          413 a          549 c          622 g          509 t
ORIGIN      
        1 tcgtcgccaa ccgacagctt cgtgaaggcc gcatcggcgt cgagggcggg gtcccgcttg
       61 cggaggtcga ggagcgtctt gtaaaacgga ctgcggtcaa aatgccggaa ccggatcgag
      121 tccttttcaa aaaaagagat cgcgcgcaga accggctctt cctggccact gtagatcagg
      181 ggaatattct cgggaagcgt ctgggtgaga acggcaaagg gcgcatgtgc agcgccgggg
      241 aaggtattat agtcggcgcc gttccagctg ttttcgtcat gattgctggt aaaatacatt
      301 tcaagcgttc ccttcgggta ggtttgttgc tcgcgctcca tcacgctgtc gaggctttgt
      361 gcgggcagtt cccctttcgc gaccctgatc attgtatgga aataatccca cggatagagc
      421 gcatcgaagc cattctccgc gaggtaaggc ttgtcgcctt ccgccaacat gaacagcgat
      481 ttcatttttc ttagccgggc aatacaggtc cgccagaaat cgcccggaac gttccaggcg
      541 acatcgcatc tgaacccgtc gatattcgtt tcccggatcc agtattgcat tgaggcgatc
      601 atactgtccc gtagctccgg gttgccgtaa tccagctggc gggtgtcggt ccaatcaaaa
      661 ggaacggcag ccttgccggt gctatctttt ttatagaagt cggggtgttc ggttagccat
      721 cggttgtccg cgcccgtgtg gttggggacc cagtcaagga ttactttcat gcctttggcg
      781 tgtgccgccc tcacaagttc ccggaaatcg tttaacgtgc cgaactcagg attgacggcg
      841 gtgtaatcgg ccacggcgta ataactgccg agggtacctt tccggtccac cttgcttatc
      901 gggttgatgg gcatgatcca caggatgtcc acacccattg ccctcaggcg gtcgaggttt
      961 ggttggaatg cgctgaaagt tccttctctg gtgtactggc ggatattcac ttcatagata
     1021 ttcgatcgct tcagccaggc agtgtggcga aggggggcct gggacaaagc ggcggaagcg
     1081 agcaagaggc agcagaagat cagaacggtg tttttcatta tctcgagggc tttttttctc
     1141 aagttacaca aatagttcaa aggaccgttg tgtcggcgga gtaacacgca ttgccatcca
     1201 ataggctaca ggatctgtac atatccttcg gcgccattca gccggacacg ctggccgtcc
     1261 cggatcagcc gggtggcgcc attaacaccg acgaccgccg gcaggccata ttcgcgggca
     1321 atcacggctc cgtgggtcat gaggccgccg acttcggtga caagcccttt tatgtagacg
     1381 aaaagtggcg tccagctcgg atcggtgaaa gtcgtgatca atatgtcgcc ggctgcgagg
     1441 tcggcatctt ccattcgcag gatgacgcgc gcccggccct ctacaatgcc gctggagact
     1501 ggcaggccgg ggaaggctcc cgggggaagg ttttcacgat cgtatgaacc ggtaatgatt
     1561 tcgccatcgg aagtgatcac tcgcggcggg gtcagttttt cgaatgttgc gtgctctgcc
     1621 tttcgtgcgg cgataaggtg gtggtccagg cgacctgtgc ggaccacttc agcgagctct
     1681 tcgaaggtca ggtagtatat gtcttccttt ttgtgaatga tcttgtttct gacgaggtga
     1741 ttggcttcat tcagcaacgc ctgtttgtat gcaaaaaggt ggttaatgat gcggtacttg
     1801 gggtactcgc ggaagccgat ggtattcctg agaagcgcga tcgtctggcc ggtctcttcg
     1861 gccttgcggt cgccgtcggg cagcagcttc agccgatgca aaagctctgc cgttttcgtc
     1921 aacgcctgtt gccggcccgc ttcaaatttc cgatgtccgg cgccggcatc gaggtttttg
     1981 atattgctga ggatgagcgg gagaagcatt gtcggttgct cactccaccg cgtcctcgtg
     2041 atatcgatct cgccggaaca ccgcattccg tacttgtcga gccaggttcg aat
//
LOCUS       DATGKE010000295         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1372710, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000295 DATGKE010000000
VERSION     DATGKE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372710"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..>2270
                     /locus_tag="VKU83_05065"
     CDS             388..>2270
                     /locus_tag="VKU83_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92952.1"
                     /translation="MFRSFFTIGWRNLRKNRTYTAINVLGLSLGIACGLLIFALVTYH
                     LSFDDFHPGKDRIYRVVTEFHDEVADFSPGAPTPLGKAFRKDLDDAEAVARIIGYSNS
                     LISIPAVGEVRKFQEEKGVAYAEPAYFDIFHFPLAEGTMLQHPGQALITERLAKKYFG
                     DVQSALGKVIRVDNRTDFILTGILRDIPVNTDRREEIYLSYDNLRDRNTRLASDSSWG
                     SVYSGNMCFVRLRPSVNRVKVDSGLARMGRTYQKGRDAQTTIFRLQPLSDIHFNADFD
                     GYVDKKYLWALFFVGVFIVLTACVNFVNLATAQALNRSTEVGIRKVLGGLRGQLFWQF
                     ITETAFIAVLAATAGYCLAVLALPTVNTLFKSEISLLWLSDPASLAFLGGLLVAVIFL
                     SGSYPGLVLSRFRPVAALKSKLSQKEIGGFSLRRILVITQFTISQLLIIGTIVIAAQL
                     RYVKNKDLGFDKQTIVMLALPGHDPAKLRSMRAQLEEIAGIEAVTFCYTAPASESNGT
                     TNVRFDNRAEDEHWEVNKKTADDQYLRTFGLKLIAGRNLFPADTVREFLINETMVARL
                     NLGTASEALGKRLVINRKAGPIVGVVRDFYNSSLRGSISPIAIFADTSAYSQCAVKLD
                     PAH"
BASE COUNT          482 a          596 c          655 g          537 t
ORIGIN      
        1 gtggcggtcc gggtgaaagt tatcgtagct atactgctga tagacgataa gcaggatagc
       61 aataccgatg gtaaatccga tgatcagtcc accgacatta atagttgtgt gcagcttatt
      121 acggacgagc tggcgccagg cgatcttcca atagttcttg agcatacgca gtagcgtttt
      181 gcctattggg gctaaaaaga tgccattccg ggagcggtta gtaaccagac ctttagccgg
      241 tcatactcga gcgaagctgt gcggttccga tacagccgta ctgtccgaaa gcggacagtc
      301 gccgataaag ctccggtcca aatgcatgat ctccaatttg ctgtatgatt ggtatcgtgt
      361 tggcagctcc ggggaataac cacacttatg tttcggagct tttttaccat cgggtggcgg
      421 aacctgcgga agaacaggac gtataccgcc atcaatgtgc tgggcttgtc gctgggcatt
      481 gcctgcggcc tgctcatctt cgccctggtg acctatcacc ttagcttcga tgatttccac
      541 cccggcaagg accggatcta tcgcgtcgtc accgaattcc atgacgaggt agcggatttt
      601 tcgccggggg cacccacgcc gttgggcaag gccttccgca aggatctcga cgatgccgaa
      661 gcggtggccc ggatcatcgg ctatagcaat agcctcatca gtattcctgc cgttggtgag
      721 gtccgcaaat ttcaagagga gaaaggggtc gcgtatgccg aacctgctta tttcgacatc
      781 tttcatttcc cgttggcgga agggacgatg cttcagcatc ccggacaggc gctgatcacg
      841 gagcggctgg ctaaaaaata tttcggtgac gtccaatctg cgctgggcaa ggtgatccgg
      901 gtagataacc ggaccgattt tatcctgaca ggtatcctgc gggatattcc ggtcaatacg
      961 gaccgccggg aggagatcta tctttcctat gacaatttga gggacaggaa cacacgactg
     1021 gcgagcgaca gcagctgggg aagcgtctat agcgggaaca tgtgttttgt gcggttgaga
     1081 ccttcggtga accgggtgaa ggtcgacagc ggcctcgccc ggatggggcg cacctatcaa
     1141 aaggggcggg atgcgcagac gaccattttc cgtctgcagc cgctttccga tatccatttc
     1201 aacgccgatt tcgacggcta cgtcgataaa aaatacctgt gggcattgtt ctttgtcggc
     1261 gtattcattg tgctcaccgc ttgtgtgaat tttgtgaatc ttgctaccgc acaagccctg
     1321 aaccgttcca cggaagtagg gatacggaaa gtgcttggcg ggctgcgcgg acagcttttc
     1381 tggcagttca tcacggagac cgctttcatc gccgttcttg ccgcgacggc gggttattgc
     1441 ctggccgttt tggccttgcc tacggtgaat acgctgttca agagcgagat cagcttgcta
     1501 tggctgagcg atcctgcttc gctggctttc ctgggggggt tactcgtggc tgtcatcttc
     1561 ctttcgggtt cctatcccgg tctggtgttg tcgcggttcc ggccggtcgc tgctctcaag
     1621 agcaaattat cgcaaaaaga gatcggcggt ttttcgcttc gacgcatttt agtgatcact
     1681 cagttcacca tatcgcagct gctgatcatc ggcaccatcg tcattgccgc gcagcttcgt
     1741 tacgtaaaga ataaagacct gggtttcgac aaacagacga tcgtaatgct ggccctaccc
     1801 ggtcatgacc ccgcgaagct tcggtcgatg cgtgcgcagc tggaggagat tgcgggcatc
     1861 gaggccgtca cgttctgcta tacagcgccg gcgtcggaaa gcaacggcac taccaatgtg
     1921 cgcttcgaca accgggccga ggacgaacac tgggaggtga ataaaaaaac agcagacgac
     1981 caatacctgc ggacctttgg gctgaaactc attgccggca ggaacctgtt cccggcggac
     2041 acggtgcggg aattcctgat caatgagacg atggtggccc ggttgaacct gggcacggca
     2101 tccgaagcgc tcggaaagcg gctcgtcatc aaccgtaaag ccgggcctat cgtaggggtt
     2161 gtccgcgatt tctacaacag ttcgctgcgc ggttcgatct cgcccattgc cattttcgcc
     2221 gatacaagtg cgtatagcca gtgtgccgtg aagctcgatc cggcgcatat
//
LOCUS       DATGKE010000296         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_348724, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000296 DATGKE010000000
VERSION     DATGKE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348724"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..546)
                     /locus_tag="VKU83_05070"
     CDS             complement(169..546)
                     /locus_tag="VKU83_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92953.1"
                     /translation="MQTTRDDMQVRWWNLEARLAERFGKKPDIETILFLIGVQEFGDI
                     RKKFTKEEKQDLMHIAVCSLFAQSGYYELERVDEEGWPHFNQQKALPDMTMAEQENFL
                     KDHILLYFERSEIELNNAEPDSL"
     gene            complement(561..893)
                     /locus_tag="VKU83_05075"
     CDS             complement(561..893)
                     /locus_tag="VKU83_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HLI92954.1"
                     /translation="MIDPTMVTTSTALDGHRVSRNLGVVRGITVRSRSVLGNMAGAFQ
                     TLFGGQISIYVDLCEKTREEAFLYMLQHAAERGANAVINMRYDANEVMNGVTEVLAYG
                     TAVIVEKI"
     gene            1111..>2361
                     /gene="serS"
                     /locus_tag="VKU83_05080"
     CDS             1111..>2361
                     /gene="serS"
                     /locus_tag="VKU83_05080"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLI92955.1"
                     /translation="MLQVAFIRQNVDFVKERLAVRNFKETDLVDDIVRLDDQRRKLQL
                     ESDTAQSGINALSREIGQLMAKGQKTEAEAKKQEVASLKASQTPIAEQLSSVEEKLNA
                     VLVRLPNLPAAAVPKGRSAEDNETIREGGSHPALAGEAVPHWDLAKKYNLIDFELGNK
                     ITGSGFPVYINKGAKLQRALIQYFLDFNTAAGYTEYQPPILVNEASARGTGQLPDKEG
                     QMYYVCEDNLYLIPTAEVPVTNIYRDEMLKESQLPIKMTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIVDPAKSYEVLEEMVLHVERLLQSLELPYRILRLCGGDMGFTSA
                     MTYDFEVYSAAQQKWLEVSSVSNFESFQTNRMKIRFKDASGKTILAHSLNGSSLALPR
                     IVACLLEDHQTDGAIVLPEPLHSYF"
BASE COUNT          594 a          647 c          598 g          522 t
ORIGIN      
        1 aattgtcgcg atgcaaaggc gtggaatcgg acagacggat caccagtata ccctcgttag
       61 taacgatctc tacatcccgg tgcaggtctt tttttcgcaa gccattggac aacctgggat
      121 tgcaggagat caacaggagc aagatagccg ctgctggcag tcttcttatc ataagctatc
      181 aggttctgca ttgttgagct ctatttccga ccgttcaaaa tacaggagaa tatggtcctt
      241 caggaaattt tcctgctccg ccatggtcat gtccggcaac gccttctgct gattgaaatg
      301 gggccacccc tcttcatcca cccgctccag ctcataataa ccgctttgcg caaacaggga
      361 acatacggca atatgcatga gatcctgttt ctcttccttg gtgaattttt ttcggatatc
      421 cccgaattcc tgcactccga tgaggaacaa aatagtttct atatccggtt tcttcccgaa
      481 ccgctccgct aaccttgctt ccaggttcca ccaccgcacc tgcatgtcgt ctcgtgtagt
      541 ttgcatcccg caaaggtaag ctatatcttc tctacgatca ccgccgtccc gtaggccagg
      601 acctctgtga cgccattcat cacctcattg gcatcgtacc gcatattgat cacagcattc
      661 gcgccccgtt cggccgcatg ctgcagcata tacaggaatg cctcctcccg ggtcttctca
      721 cacagatcaa cgtagatgga tatttgcccg ccgaacaggg tctgaaaggc gccggccata
      781 ttgcccagaa cactgcgaga gcgtacagtg atgccgcgaa caacacccag gttacgcgaa
      841 acccggtgcc catcgagcgc ggtgctggtc gttaccatag tagggtctat catataaaga
      901 ggttgttatg gataaaatta cgtctttttg ccgacgtagg aggcatgtcc gccaggacaa
      961 acacaaccga gcgcagcgaa ggttgcgaac gagaaggagc gtggcgaagc agctccgacg
     1021 aggttctcta tgtgcgggcg atgcctaatt ttttccggag gggcatcgcc cgcacataat
     1081 cagtagtaac tttaccgcaa aatccagaaa atgttacaag tggcatttat ccgtcagaac
     1141 gtagacttcg taaaggaaag gctggctgtc aggaacttca aggaaaccga tctggtggac
     1201 gatatcgtcc gcctcgatga tcagcgcagg aagctgcagc tcgaatccga taccgcccaa
     1261 tccgggataa atgctttgtc cagggagatc ggccaactca tggccaaagg ccagaaaacg
     1321 gaggccgaag caaaaaagca ggaagtagcg tcgctaaagg catcgcagac gccgatcgca
     1381 gaacagctct cctcggtaga agagaagctg aatgccgtac tggtccgtct gcccaatctg
     1441 cctgctgctg ccgtaccaaa gggaagatct gcggaagata atgaaacgat ccgtgaagga
     1501 ggcagccatc cggctttggc cggcgaagcc gttccacatt gggaccttgc aaaaaaatac
     1561 aatctcatcg atttcgagtt ggggaacaag atcacgggaa gcggattccc ggtatacatc
     1621 aacaagggag cgaaactgca acgggcgctc atccaatact tcctcgattt caacacggcc
     1681 gcgggctata cagaatatca gccgcccatc ctcgtgaatg aggcttccgc ccggggcacg
     1741 ggacagcttc ccgataaaga aggtcagatg tactatgtat gcgaggacaa cctttacctc
     1801 atacctacag cggaagttcc ggtgaccaat atctatcggg acgagatgct gaaagaatcc
     1861 cagctcccga taaagatgac ggcttacact ccctgcttcc gccgggaagc gggcagctac
     1921 ggcaaggatg tccgtggtct taaccgcctc catcagttcg acaaggtgga gatcgtccag
     1981 atcgtagatc ccgcaaaaag ctacgaagtc ctggaagaga tggtgctgca tgtcgagcgg
     2041 ctcctgcaat cgctggaact gccttatcgc atccttcggc tatgcggcgg agatatgggg
     2101 ttcacttcgg ccatgaccta tgacttcgag gtgtatagcg ccgcgcagca aaaatggctt
     2161 gaggtcagct cggtcagtaa tttcgaatca tttcagacca accggatgaa gatccgcttc
     2221 aaagatgctt cgggcaaaac cattttagcg cactcactca atggcagctc gctcgccctg
     2281 ccccgtattg tagcctgtct gctggaagac catcagacgg acggagccat cgtgttgccg
     2341 gaaccgttac attcctactt t
//
LOCUS       DATGKE010000297         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_639113, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000297 DATGKE010000000
VERSION     DATGKE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639113"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1082)
                     /locus_tag="VKU83_05085"
     CDS             complement(189..1082)
                     /locus_tag="VKU83_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92956.1"
                     /translation="MNYKSLAVVVLAFIPNALLLAQRDQGYQTLRMTSAFTSFPDSAR
                     TNGFLDGDSVFRPAAGHYDDSSVLLVTPLHPRWRRKVDLIFWFHGWHNNIDTALQFYG
                     LARQFADSKCNAVLVLPEAAKNAADSYGGKLRRDGDFRNLVGDVMKKLSAARVVPRHA
                     EAGHIVLACHSGGYSAVADILAHGQQEVEAVWLFDALYGRLPVFMDWIRTDKRHRFVH
                     WFTNHGGGTDEMSDTLMRELGLQHTGFLLTDEASLKPAMIRDNRVLFIHSPRQHNVII
                     NDPDDFELLLQNAVVIRCGGY"
     gene            1195..2100
                     /locus_tag="VKU83_05090"
     CDS             1195..2100
                     /locus_tag="VKU83_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HLI92957.1"
                     /translation="MFLLILGILLLVGSYTIARRTDNLRRYMAPLRILGLILALIGLS
                     TASLVQIDAGEIGVQMLYGKVQNDVLYSGLHFINPLMEVQKMDVKTQNYTMSAVRDEG
                     AKEGDDAVRALTSDGLEVVVDVTVLYRVLPGDAPVILRQTGLDYTDKIVRPLARTRIR
                     DNAVYYDAVSLYSSKRDEFQRRIFQSIAGEFEKRGLLLEQLLIRNINLPQAVKTSIEQ
                     KMTAEQDAQKMQYVLQKEQQEAQRKRVEAQGIADYQRIISESLTDRQLQYESIKAQLE
                     IAKSANTKVIIMGKGSAPVMLDTKN"
     gene            2157..2429
                     /locus_tag="VKU83_05095"
     CDS             2157..2429
                     /locus_tag="VKU83_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLI92958.1"
                     /translation="MPTIHCVISGRVQGVNYRAATRDMASQLGLTGWVRNTLDGNVEL
                     TATGPEAALRKLIEWCRQGPPHAAVSNVSTERLPETPFTGFEIRRG"
BASE COUNT          571 a          776 c          643 g          506 t
ORIGIN      
        1 ggcatccacg atggccaacc accggtcggc gactttattg ccgccatttt ccggcaattg
       61 gagcaaggaa agaacgaact tacctttgga ttcagcgagc aaatgatcaa ggctggcccc
      121 gaccaactcc gccaggcgtt cgcgcgaatg aacccgtgat cgggcccgct ctgcaaaagc
      181 taccccggtc aatagccgcc gcaccgaatg accaccgcat tctgaagcag cagctcaaag
      241 tcgtccggat cgttgatgat cacgttatgc tgcctgggac tgtgaatgaa cagtacccgg
      301 ttatcccgga tcatcgccgg cttcaatgag gcctcgtccg tcagcaaaaa gcctgtatgc
      361 tgcaggccaa gctcccgcat cagcgtatcg ctcatctcgt ccgtgccgcc gccgtgattc
      421 gtaaaccaat gtacgaaacg atgccgcttg tccgtcctga tccaatccat gaagacgggc
      481 aaacgaccat acagcgcatc gaacaaccat accgcctcca cctcctgctg cccatgcgcc
      541 aggatatccg ccacggccga gtaaccgccg ctatggcagg ccagtacgat atgcccggct
      601 tccgcatgac gcgggacaac cctggcagcg ctcagcttct tcattacgtc tccaacaagg
      661 ttcctgaagt caccatcgcg tcgcaacttt ccgccataac tatctgccgc gtttttcgcg
      721 gcttcgggca agacaaggac ggcgttacac ttcgagtcgg caaactgcct tgccagaccg
      781 taaaactgca acgcggtgtc gatattgttg tgccagccat ggaaccagaa tatcaagtcc
      841 acttttcgcc gccacctggg atgcaatggc gtcaccagca gcacactact gtcatcgtaa
      901 tgccccgcgg cagggcgaaa tacgctgtca ccgtcaagga acccgttcgt ccgcgccgag
      961 tccgggaagc tcgtaaacgc agaggtcata cgaagggttt gatacccttg atccctttgc
     1021 gccagcaaca gcgcgttggg aatgaacgcc agaacgacga ccgctagcga tttgtaattc
     1081 atgctggtcc gattattatc cgaatgtacg aaaaccttcc cgacgcttgc cgctcctcat
     1141 tctgttgccg gccccgacct gtttcaggtt atctttatgg aaattttaac cccaatgttt
     1201 ctcctcatcc tgggaatcct cctgcttgtc ggaagctaca ccatagcccg caggacagat
     1261 aaccttagac gttatatggc gcccctccga attcttggtt tgatcctggc ccttatcggg
     1321 ctctccaccg cttcccttgt ccaaatcgat gccggcgaga tcggcgtcca gatgttgtat
     1381 ggcaaggttc aaaacgacgt gctctatagc gggctgcatt ttatcaatcc gctgatggag
     1441 gtgcaaaaga tggatgttaa gacgcagaac tacactatga gcgcggtccg cgacgaaggc
     1501 gccaaggaag gtgatgatgc ggtccgcgcg ctgacatccg acggcctcga agtcgtagtc
     1561 gatgtgaccg tcctgtaccg ggtccttcca ggcgacgcgc cggtcatcct ccgccagacc
     1621 ggcctggatt ataccgacaa gattgtgcgg ccgctggccc gaacccggat ccgcgacaac
     1681 gccgtctatt acgacgcggt ctccctttat tccagcaaac gtgatgaatt ccagcgccgc
     1741 attttccagt cgatcgccgg tgagtttgaa aaacgcggcc tccttctcga acaactgctg
     1801 atccgcaata tcaacttgcc acaggcggtc aaaacgagca tcgaacaaaa aatgactgcc
     1861 gaacaggacg cgcagaagat gcaatacgtc ctccaaaaag agcagcagga agcccagcgg
     1921 aagcgcgtgg aagcgcaggg tatcgccgat tatcaacgca tcatcagcga aagcctcacc
     1981 gaccggcagc tgcaatacga gtcgatcaaa gcccaactcg agatcgccaa atccgccaat
     2041 accaaagtga tcatcatggg caagggctcc gctcccgtca tgctggacac caaaaattaa
     2101 ccggggtcta tcgcaccttt gcaggaattt cgttcatttg cgggaatttt gttcagatgc
     2161 cgacgatcca ttgcgtcatc tccggccgcg tccagggcgt gaactatcgc gccgccaccc
     2221 gtgacatggc cagccaactt ggcctcacgg gctgggtccg caacaccctt gatggcaatg
     2281 tcgagcttac tgccaccgga cccgaggccg cgctgcggaa gttgatcgaa tggtgccgtc
     2341 agggcccgcc gcacgccgct gtttccaacg tcagcaccga gcggctgccg gaaacaccgt
     2401 tcaccggatt tgagatccgg agggggtgat ccggttcgtc tttgctttcg tgggctcccc
     2461 aaccgggcag atcaaaagcg aagcccgcaa tggcag
//
LOCUS       DATGKE010000298         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_537240, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000298 DATGKE010000000
VERSION     DATGKE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537240"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="ccoO"
                     /locus_tag="VKU83_05100"
     CDS             <1..1323
                     /gene="ccoO"
                     /locus_tag="VKU83_05100"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HLI92959.1"
                     /translation="APSWGGMLNGLFTLRGAWDKVREDPVLKFLVVAITCYGMATFEG
                     PMLSLKNVNAISHFTDWTIAHVHIGALGWNGFLTFGILYWLIPRMWNTTLYSKKLAGT
                     HFWIGTIGIVLYAIPLYWAGFTQAMMWKSFTDAGQLKFQFLETVTHILPMYIARSLGG
                     ALYLTGALLMVYNLRKTIAKGSFIANEAAEAPALAPAVESGGRTYWHRWIERRPMQML
                     MFSLIIVAIGGLIELIPTFLVKSNIPTISSVRPYTPLELQGRDIYVREGCYLCHSQMV
                     RPFRDEVARYGEYSKAGEFVYDHPFQWGSKRTGPDLARIGGKYPDSWHYNHLLDPSSM
                     SPGSIMPSYVWLFDHEIDTTDTYKKIKAMQTLGVPYPAGYADQANRDLVDQEREIKAS
                     LKGDKIATGQNKEIVALIAYLQRLGKDIKAVPKTQTAAAGDGREIMGN"
     gene            1339..1497
                     /locus_tag="VKU83_05105"
     CDS             1339..1497
                     /locus_tag="VKU83_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92960.1"
                     /translation="MKFIHYIEKVSGVSIYGLSSLLLFSGIFLIMLWYAIKVDKRMIE
                     EARKMPLD"
     gene            1522..>2483
                     /locus_tag="VKU83_05110"
     CDS             1522..>2483
                     /locus_tag="VKU83_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HLI92961.1"
                     /translation="MKPPSSLSNPTIETLIAVAGGLLIVIISLASSLLKVARRVRAAE
                     KEQDSQKSPQLTDSTAIAATTQVKTAGGPLAGAATVTGQVINGVSDTAFYLVTSVIIA
                     EAVVILYLLFNLRRLVRQQAAVATPPWSFAKWWAKVNRFRPITQEADLDLGHDYDGIR
                     ELDNRLPPWWLYGFYLTIIFAGVYLWRYHIAHSAPLPAEEYQIAVREAEVKKAAFLKA
                     TANNVDENTVKLLTGRSDLDSGKAIFQGVCFACHGKYGEGGVGPNLTDKYWLHGGTVN
                     EVFKSIKYGWPEKGMKSWKDDYSPQQIAQLASYVKSLAGTNPPNA"
BASE COUNT          577 a          685 c          698 g          523 t
ORIGIN      
        1 gcgccgagct ggggcgggat gctcaacggt ctgttcacgt tgcggggcgc ctgggacaag
       61 gtccgcgagg atccggtact gaagttcctg gtggttgcga tcacctgcta cggcatggcg
      121 accttcgagg ggccgatgct cagcctgaaa aatgtgaacg ccatttcgca ttttacggac
      181 tggaccattg cgcacgttca tatcggggca ttaggttgga atgggttttt aacgttcggc
      241 attctttact ggcttattcc ccggatgtgg aacaccaccc tctactcaaa aaagctggcc
      301 gggacgcatt tctggatcgg cacgatcggc atcgtcctat acgccatccc cctgtattgg
      361 gcgggcttta cccaagccat gatgtggaag agtttcaccg atgcggggca gctcaaattc
      421 cagttccttg aaaccgtcac acatatcctt ccgatgtata tcgcaaggag tcttgggggc
      481 gcgctctacc tcaccggcgc attgctcatg gtgtacaatt tgaggaagac gatcgcgaaa
      541 ggaagtttta ttgccaacga agcggccgaa gcgccggccc tggcgccggc ggtcgaatca
      601 ggcggccgca cttactggca ccggtggatc gaacggcggc cgatgcaaat gctgatgttc
      661 agcctgatca tcgtggcgat cggcggcctc atcgagctca taccaacctt ccttgtcaaa
      721 tcgaatatcc ccaccatcag cagcgtcaga ccgtatacgc ctttggaatt gcagggccgg
      781 gacatttatg tccgggaggg ctgctatctc tgtcactcgc aaatggtgcg gccattccgg
      841 gatgaggtgg cgcgatatgg ggaatactcg aaggccggtg aattcgttta cgaccaccct
      901 ttccagtggg gttccaagcg gacggggccg gatctcgcgc ggatcggtgg gaaatatccg
      961 gacagctggc actataatca cctgctcgac ccatcctcca tgtcgcccgg atccatcatg
     1021 cccagttatg tctggctatt cgaccatgaa attgatacca cagataccta caagaaaata
     1081 aaagccatgc agacgctggg agtgccttat ccggcaggtt atgccgacca ggccaacagg
     1141 gatctcgtgg atcaggagcg ggaaatcaag gcctcgctga aaggcgacaa gatcgcgacc
     1201 ggccaaaata aggagattgt cgcgctgatc gcttacctgc aaaggctcgg gaaggatatc
     1261 aaagcagtgc cgaagacgca aacagccgcc gcaggcgacg gcagggagat catgggaaac
     1321 taaatacagg ctacgtaaat gaagttcatt cactatatcg aaaaagtcag cggcgtcagc
     1381 atctatgggc tttcttccct gttgctgttc tcaggtatct tcctgatcat gctatggtac
     1441 gcgataaaag tagacaaacg catgatcgaa gaggcgcgca agatgccgct ggactagctg
     1501 atttctttaa aaagtatgat tatgaaaccg ccatcatccc tatctaatcc aaccatcgaa
     1561 acgctgatcg ccgtggcggg cggtctcttg atcgtcatta tctccctggc ctcttccctg
     1621 ctgaaggtcg cccggcgggt ccgggcagcg gagaaggagc aggactccca gaagtccccg
     1681 caacttacgg attccactgc catcgccgcc accacccagg tcaagacggc cggaggtccg
     1741 ctcgcggggg cagccaccgt tacaggccag gtgatcaacg gggtgtcgga tacggctttt
     1801 taccttgtca ccagcgttat tatcgcggaa gcggtggtga tcctttatct cctgttcaac
     1861 cttaggcggc tcgtacggca gcaggcagcc gtcgccacgc ctccctggtc cttcgcaaaa
     1921 tggtgggcaa aagtcaaccg tttccgaccg atcacgcagg aagctgacct ggacctcggt
     1981 catgactacg acggaatccg cgaactggac aaccggctcc ctccctggtg gctatacggc
     2041 ttctatctta cgatcatttt tgccggcgtc tacttatggc ggtatcatat cgcacacagt
     2101 gcgccgttgc ccgccgaaga ataccagatt gccgtcaggg aagcggaagt caaaaaagca
     2161 gcatttctaa aggcaacagc caacaacgtc gatgagaata ccgtcaagtt actcactggc
     2221 cggtccgatc tggattcggg aaaggctata ttccaggggg tatgttttgc ctgccatgga
     2281 aaatacggcg agggcggggt tggtccaaac ctcaccgaca aatactggct ccacggtggg
     2341 accgtcaacg aggtgttcaa gagcatcaaa tacggctggc cggagaaagg catgaagagt
     2401 tggaaggacg actattcgcc gcagcagatc gcgcagctgg ccagctacgt gaaatccctc
     2461 gcagggacga atccgccaaa tgc
//
LOCUS       DATGKE010000299         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1313247, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000299 DATGKE010000000
VERSION     DATGKE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313247"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VKU83_05115"
     CDS             <1..680
                     /locus_tag="VKU83_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92962.1"
                     /translation="LTLMLGWIGYYDKGSYQRFESINPWLAQPDSLQNTQVQEGYFGI
                     KGSLDKHFSYSIKAGVQLYNNMPLFVNDSANGGKDFQIRYANSMQARQLHGELSYLYG
                     EQFQATASLNLEQYLDIKGQPKAWGLLPVEFKTNLKWEVLKDFWAKLDLFTFEGAPYR
                     APDGVAFTGPNGFDMNAGVELRILKQLNLWFQMNNIFNDQYERWHQYPVYGFNVAFGI
                     VFSFPGK"
     gene            744..1346
                     /locus_tag="VKU83_05120"
     CDS             744..1346
                     /locus_tag="VKU83_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92963.1"
                     /translation="MLQVLNAYLFQHRSISIPGLGTIYLETYPASADVADRTILPPAY
                     RFRFDKYFDAPEREFFAYLAVQGKMLDFEAIKWYNEFSFDLRNRIRNEDEVPWEGVGV
                     LKKDESGNILLESAAAPAFYSSPVPAMRVNRQDAVHTLLVGDRERTSGEMNEWLQEKV
                     VSRRRISWWVVALVLAVIGLAILAWHYYAYGWSTANQSAL"
     gene            1361..2248
                     /locus_tag="VKU83_05125"
     CDS             1361..2248
                     /locus_tag="VKU83_05125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI92964.1"
                     /translation="MSLIHYNACPSCHSDEIKPVLTATDYTVSHESFQIWQCQSCTLR
                     FTQDVPDATAIGPYYHSEDYISHSNTTRGWVNMLYHKVRKLTLSDKRRLIQSATRKRE
                     GSLLDIGAGTGAFVGYMKMQGWEATGLEPEAVARERALADHHVSLLDMASLESLDDNM
                     FDAITLWHVLEHVHDLHHYLDRLRALVKKGGRIFIAVPNYTSYDAVAYGAAWAAYDVP
                     RHLYHFSPDAMEVLLNRHQLQLCYTQPMWYDSFYISMMSEKYRKGNMAGAVWRGLISN
                     LQAFIDKSKCSSLIYVAGR"
BASE COUNT          552 a          604 c          647 g          546 t
ORIGIN      
        1 gcttaaccct tatgttgggt tggatcggct attatgacaa gggatcatat cagcggttcg
       61 agtccatcaa tccctggctc gcgcagccag attcgctgca gaacacccag gtgcaggagg
      121 gatactttgg cattaaggga tcgctggaca aacatttcag ctattccata aaggctgggg
      181 ttcagctcta taacaatatg ccgctgtttg tcaatgacag cgcaaatggc ggaaaggact
      241 ttcagatccg gtatgccaat tcaatgcagg ccaggcagct gcacggcgag ctgagctact
      301 tgtacgggga gcaattccag gcgacggcga gtctgaatct cgagcagtac ctggacatta
      361 aagggcagcc gaaggcatgg ggattactgc ccgtcgaatt caagaccaac ctgaaatggg
      421 aggttttgaa ggacttctgg gcgaagctgg acctgttcac tttcgaaggc gcgccgtatc
      481 gcgccccgga cggcgtcgcc ttcaccggac caaatggttt cgatatgaac gctggcgtcg
      541 aactccggat tctgaaacag ttgaaccttt ggttccagat gaacaatatc ttcaacgatc
      601 agtacgaacg gtggcatcaa taccccgtct acggattcaa tgttgctttc ggaattgtct
      661 tttcttttcc cgggaaataa ggccccaaac cgaaaaaata gcccgaaata cgtagattcg
      721 cagctcagca aaaggaatat gtcatgttgc aggttttgaa tgcctatttg ttccagcacc
      781 gcagtatcag catacccggc cttgggacga tatacctgga gacgtatcct gccagtgctg
      841 atgtggcgga caggacaatc ctgccgccgg cgtaccggtt ccgattcgac aagtatttcg
      901 atgcccccga gagggagttt tttgcatacc tggctgtaca gggcaaaatg ttggattttg
      961 aggccatcaa gtggtacaac gaattttcgt tcgatctgcg caatcgcatc cggaatgaag
     1021 atgaggtccc atgggaaggc gttggggtgc ttaaaaaaga cgagtccggt aacatcctgc
     1081 tggagtcggc agcggcgccg gccttttatt cttcccccgt gccggcaatg cgcgtcaatc
     1141 gccaggacgc agtgcataca ttgctggtcg gggaccgcga gcgaaccagc ggcgagatga
     1201 atgaatggct ccaggaaaaa gttgtgtccc gaaggcggat ttcctggtgg gttgtcgccc
     1261 tggttcttgc ggtgattggc ctcgccatcc tggcctggca ctactatgct tatggctggt
     1321 ccacagccaa tcaatctgca ttatagcgtt tgcaaccgat atgtccctga tccactataa
     1381 cgcttgcccc tcctgtcatt cggacgaaat taaaccggtt ctgaccgcga ccgattacac
     1441 cgtctcacat gaatctttcc agatctggca gtgccagtcc tgcaccctgc gtttcacgca
     1501 ggacgttccg gatgcgacgg caatcggccc ctactatcat tccgaggatt atatcagcca
     1561 ctccaacacg acgcggggat gggtcaacat gctttaccat aaagtccgga aactcacctt
     1621 atcggacaaa cggcggctga tccagtccgc caccaggaag agagagggca gtctgctgga
     1681 tattggcgcc ggtacgggcg cctttgtggg gtatatgaag atgcagggat gggaggcgac
     1741 cggtctggag ccggaggcgg ttgcacggga gcgggcgctg gcggaccacc acgtatcctt
     1801 gctggacatg gccagcctgg aatctcttga tgacaacatg ttcgatgcca ttaccctctg
     1861 gcacgtgctc gagcatgtgc atgacctcca ccattatctt gatcggttaa gagcgctcgt
     1921 aaagaaaggg ggtcggatat ttattgcggt gccgaactac acgtcgtatg acgccgttgc
     1981 ctatggtgcc gcatgggcag cctacgacgt cccgcgtcat ttgtatcatt tttcgcctga
     2041 cgccatggag gtcctgctga accggcatca attgcaactt tgctatacgc agccaatgtg
     2101 gtatgacagc ttttacatca gcatgatgag cgagaaatac cggaaaggta atatggcggg
     2161 cgcggtctgg agaggactta tatcgaatct ccaggcattt atcgataaat caaagtgcag
     2221 ctcgctgatc tatgtggccg gcaggtgaaa tctcagggag aaacccggat ctcgtaaata
     2281 ttaggccata gttttcccgt aacatacagc agcccggacg ccgggttata agcgatcccg
     2341 ttcaatacc
//
LOCUS       DATGKE010000300         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1490210, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000300 DATGKE010000000
VERSION     DATGKE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490210"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..340)
                     /locus_tag="VKU83_05130"
     CDS             complement(56..340)
                     /locus_tag="VKU83_05130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI92965.1"
                     /translation="MEIPVVNNPARQQFQVTVGGETASLDYRLPEGTLVLMHTEVPEK
                     LGGRGIGSALAGFSFQYARENQLRVKVYCPFVAAWVKKHAEVQDLIVNAD"
     gene            460..1218
                     /locus_tag="VKU83_05135"
     CDS             460..1218
                     /locus_tag="VKU83_05135"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HLI92966.1"
                     /translation="MKKAIYESLLASKAKGRHSLAVLVDPDNVNPSKLDELTVLAAGA
                     EVDYFFVGGSLVISNHLDEVVQHIRKNCRIPILLFPGTPAGVSRYADALLYLSLISGR
                     NPELLIGQHVISAPAVRKSGLEIISTGYMVIDGGAPTTVSYISNASPIPADKNEIALC
                     TAMAGEMLGMKLIYMDAGSGARHPVREEMIACVAGNIDVPLVVGGGIRNPERAYLNCR
                     AGADLIVVGNAIEKDPGLIREMAAAVHSIPVKAL"
     gene            complement(1219..>3648)
                     /locus_tag="VKU83_05140"
     CDS             complement(1219..>3648)
                     /locus_tag="VKU83_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92967.1"
                     /translation="QSRILPRIFLIFARTAPARPKCQNMPADYLKIAFRRLLRQRTIS
                     LINIFGLATGLAACLLIFFYVHYELGYDAYNAKASRIVRITTLVHTPESDIHIAGTPG
                     PLGSTLARECPDVEAAVRIESATFNVRLAGETVAAKNFNYSEPAVFTIFSFKFLEGSA
                     TAALREPGSIVLTRSAATTYFGKGPALGKTLMGNGQPYRVTAVIADRPANSDLPIDAL
                     VYKDWSSATTWTDDVDATYTFVLFRETPDIQRFDTRLPGLTHRFIQPELDRAGLKGYT
                     FDFEAEKLTDVHFSRDKLGDEAKGNRQFLTIFSWLAVFILLVALFNYVNLSTATAVER
                     AKEIAIRKVAGAGPRRLVRQFLLESGFLIAIAWILSFGIVLAVLPVFNRLLRTNIAFY
                     GWQPMLFLALLFPVTTFLAGGYPAFVLSRFSPLKALKGQMENAGKGIGLRKTFTVIQF
                     IIALAMLCGTIVIYRQMQFIAHRDLGASRAGITCINIPPDPAARASAPAFFQALRGEA
                     GTQGISIGSGIPSEGFQLASIVLWKGGEERRMMLNLFFADPMLLPMLDIKLAAGRNFS
                     DSLKSDRQEGWIVNQALVRAMGWKTGIGESISYEGSGIKGKVIGVVKDFYFQSLHNVL
                     APMAMIYKTDPPLAVLVKATPKELPRLRQIWRTYELAFPFDYYFLDENFSKQYDDDRM
                     MMTLFNAFTFLAVPISLTGIYGLVSLLVLRRTKEIGIRKVLGAPVGVLITLFTNDLLL
                     LIGVAAAVAFPLAAMGASKWLASYAYHTGVSAWIFVLPAAIILLLTMGVTAFRIAGAA
                     MANPVKALRSE"
BASE COUNT          804 a         1008 c         1087 g          749 t
ORIGIN      
        1 tatacacatt cggcccgtca atattgacac gcaccggtta tttggtaacc gcctgctaat
       61 cagcattaac aatgagatcc tgcacttctg cgtgcttttt cacccacgcg gcgacgaagg
      121 ggcagtacac ttttacccgc agttggttct cgcgcgcgta ctgaaacgaa aacccggcga
      181 gcgccgaacc gatgccgcgc ccgcccaatt tttccggcac ttccgtatgc atcagcacca
      241 gcgtgccctc gggtaaccga taatccagcg atgccgtttc gccacccacc gttacctgga
      301 attgttgtct tgccggattg ttaacgacgg gtatttccat ggacgtgtat ctgtctaatt
      361 tagttctaat ttcgtcgaat taccccctca aagccgaaaa gttttcgcat ttttgcctaa
      421 ccgatcaact caacgcgtga aaatttccaa ggcgagcgaa tgaagaaagc tatttatgaa
      481 tccttattgg ccagtaaagc aaaaggcagg cattcgctgg cggtgctggt cgatccggat
      541 aacgtgaacc cttcaaagct ggatgaactg accgtgctcg ctgccggcgc ggaagtcgat
      601 tattttttcg ttggcggaag cctcgtgatc tcgaatcatc tcgacgaagt ggtgcagcac
      661 atccggaaaa attgccggat tcccattctc ctgtttccgg gcacccccgc cggcgtatcc
      721 aggtatgcag acgcgctgct gtatttatcc ctgatttccg gccgcaatcc cgagctgctg
      781 atcgggcaac atgtgatctc cgcgccggcc gtccggaaaa gcgggctcga gatcatttcc
      841 acgggctata tggtgatcga cggaggcgcg ccgacaacgg tgtcgtatat cagtaatgcc
      901 agcccgatac ccgccgataa gaacgagatc gccctttgca cggcgatggc cggcgaaatg
      961 ctgggcatga agctcattta catggatgcg ggaagcggcg cgcggcatcc ggtccgcgaa
     1021 gaaatgatcg cctgcgtggc cggcaatatc gacgtccccc tggtggtggg cggcgggatc
     1081 cgcaatcccg agcgggcgta tctcaactgc agggcagggg cggacctgat cgtggtgggc
     1141 aacgccatcg aaaaggatcc tgggttgatc cgcgaaatgg cggcggcggt tcacagcatt
     1201 ccggtgaaag cactatagct attcgctacg caatgccttt acaggattgg ccattgccgc
     1261 ccctgcaatg cggaaggcgg tgacccccat cgtgagcaac aggataatgg ccgccggcaa
     1321 gacaaagatc caggctgaca cccccgtatg ataggcatag gaagcaagcc acttgctggc
     1381 ccccatggca gccagcggga aggctacggc ggcagctact ccgatcagca gcaacaggtc
     1441 gttcgtgaac agggtgatca gaacgccgac cggagcgccc agcactttcc ggatcccgat
     1501 ctccttcgtt cgccgaagca ccagcagcga taccaatcca tagatgccgg tcaggctgat
     1561 cggcaccgcc aaaaaagtaa aagcgttgaa aagcgtcatc atcatccggt cgtcgtcgta
     1621 ttgtttgctg aaattttcgt ccaaaaagta atagtcgaac ggaaaggcga gttcgtaggt
     1681 gcgccagatc tgccgcagcc ggggcaattc ctttggcgtg gcctttacca gcacggccag
     1741 gggcggatcg gtcttataga tcatcgccat cggcgccagg acattgtgga gagattgaaa
     1801 atagaaatcc ttcacgactc cgattacctt ccctttgatg cctgaccctt cgtaggagat
     1861 ggactcccca atacctgttt tccagcccat tgcccggacc agggcctggt tgacgatcca
     1921 gccttcctgc cggtcgctct tcaggctgtc ggaaaagtta cggcccgcag ccagcttgat
     1981 atccaacatc ggcagcagca tcgggtcggc aaagaaaaga ttgagcatca tccttctttc
     2041 ctcacccccc ttccaaagca cgatagatgc gagctgaaag ccttcggatg gtatcccgct
     2101 gccgatggag atgccctgtg tgccggcctc cccgcgaagg gcctggaaaa aggccggggc
     2161 cgaggcgcgt gcggccgggt cgggcggaat attgatacat gtgatccctg cacgactcgc
     2221 gcccaggtcc cgatgggcga tgaattgcat ttgccggtaa ataacgatgg tgccgcagag
     2281 cattgccagg gcgatgatga actggataac ggtaaacgtc tttcgcagcc cgataccctt
     2341 gccggcgttc tccatttggc ctttcaacgc ctttagcggg gaaaagcggg acagcacgaa
     2401 tgccggatac ccgcctgcca ggaaggtcgt taccgggaag agcagggcga ggaacagcat
     2461 gggctgccag ccatagaagg cgatgttcgt tctcaggagc cggttaaaca cgggcaggac
     2521 ggccaggacg atcccgaagg aaaggatcca ggcaatggcg atgaggaagc cggattccag
     2581 gagaaattgt ctgaccagcc ggcgtggccc tgcgccggcg acctttcgta tggctatctc
     2641 cttcgccctt tcgaccgccg tggctgtgga caggttgacg tagttgaaga gggccaccag
     2701 caggatgaat acggccagcc aggagaagat cgtcaggaac tgccggttac cttttgcttc
     2761 atctcccagc ttatccctgc tgaaatgtac gtcggtgagc ttttctgcct cgaagtcgaa
     2821 agtatagccc ttgaggcccg cgcggtcgag ctcgggctgg ataaatctgt gcgtaagacc
     2881 gggaaggcgg gtatcgaatc gttggatgtc cggcgtctcc ctgaacagga caaaggtgta
     2941 cgtcgcgtct acgtcgtccg tccaggtagt agcagacgac cagtctttat acacgagggc
     3001 gtcaatgggg agatcggaat tggcagggcg gtccgcgatc acggcggtga cccggtaagg
     3061 ttggccattc cccatgaggg tctttcccag ggccgggcct ttgccgaagt aggtagtcgc
     3121 ggcactgcgg gtaaggacga tcgaacccgg ttcacgcagc gctgctgtgg cggagccctc
     3181 caaaaatttg aaggaaaaga tggtgaaaac cgccggctcc gagtaattga aattttttgc
     3241 ggcgacggtc tcgccggcga ggcgcacatt gaaagtcgcg gactcgatcc tcacggctgc
     3301 ttccacgtcc gggcattcgc gggccagtgt ggatcccagg gggcccggcg tgccggctat
     3361 atggatgtcg gactcgggcg tgtgcacgag ggtggttatc cgcacaatcc gggaggcttt
     3421 cgcgttatag gcatcatagc caagctcata gtggacatag aaaaaaatga gcaggcaggc
     3481 ggcgagaccg gtggccaggc cgaagatatt gatcagcgag atcgttcttt gccgcaaaag
     3541 ccggcggaaa gcgattttca ggtagtcggc aggcatgttc tggcatttgg gtcttgcggg
     3601 cgcggtccgt gcaaagatga ggaagatgcg gggcaggatg cgggactg
//
LOCUS       DATGKE010000301         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1403203, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000301 DATGKE010000000
VERSION     DATGKE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403203"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VKU83_05145"
     CDS             <1..615
                     /locus_tag="VKU83_05145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HLI92968.1"
                     /translation="LVPFLASRGDFVATTAVYDGQPLPKTGDFDGLIIMGGPMSVHDE
                     AEYSWLRAEKELIGSAIRDGKKVLGICLGAQLIAEACGARVYKNKEKEIGFWPVKWAV
                     RPGFTGATADNERFFHWHGETFDLPAGAGLLASTAACVNQAFRLGDRVMGVQFHPEVT
                     AAMVRRMVGHGEPELVDGAFIQQREEILAEQPRKECMDFLADWL"
     gene            968..>2259
                     /gene="ricT"
                     /locus_tag="VKU83_05150"
     CDS             968..>2259
                     /gene="ricT"
                     /locus_tag="VKU83_05150"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HLI92969.1"
                     /translation="MGCSNCGTSKGGQPAGCKSNGGCSTGSCNRLNVYDWLANLPFSD
                     PESGCRVVEVSFNQGSRKDYFRNSTTQVFEKGDLVAVEGISGFDVGEVTLTGESVRLQ
                     LKKKGIEERNPDMKRIMRRASEKDIDIMRQNKGRERDALIRSRAIARQLKLDMKMSEV
                     EIQADGRKATFFYIADDRVDFRELIKIYASEFRLKVEMRQIGARQEAAKVGGIGSCGR
                     ELCCATWLSDFKSVNTTAARYQNLSINQSKLSGQCGRLKCCLNYELDTYLDALQNFPD
                     NAEILQVAKGAANLIKKDIFKNLMWYVLPDSNKQYPLTIERVRKIRQMNLQNMVPDTL
                     EPVEVSTGKPKETEETGFVDLVGQISLKTLEKADRKKRHGHHKHGGQGRKEGFQGRDG
                     GQSRDGGQGKEGGQGRDGGQGREAIQGRDGGRGRDGQRG"
BASE COUNT          555 a          522 c          750 g          432 t
ORIGIN      
        1 ctggttccat ttctggcatc aaggggcgac ttcgttgcca caaccgcggt ttatgacggc
       61 cagccgctgc cgaagaccgg ggattttgac gggctgatta tcatgggcgg gccgatgagc
      121 gttcatgacg aggccgaata ctcctggctg cgggcggaaa aggaattgat cggttccgct
      181 atccgcgatg gaaaaaaggt tttggggatt tgccttgggg cgcagctcat cgccgaggca
      241 tgcggtgcaa gggtttataa gaataaggaa aaagaaatcg gcttttggcc ggtcaaatgg
      301 gcggtcagac ccgggttcac cggagctacg gcggacaatg aaaggttttt tcattggcat
      361 ggggagactt ttgacctgcc tgccggggcc gggctgctgg cgtcgacggc ggcatgtgtc
      421 aaccaggcct tccggctggg agacagggtt atgggcgttc agttccatcc ggaagtaacc
      481 gcggcgatgg tgcgtcgcat ggtcgggcac ggcgagccgg aactggttga cggagcattt
      541 atccagcaaa gagaggaaat cctggctgaa caaccccgga aggaatgcat ggacttttta
      601 gccgattggc tgtgagcggc ttagggcagc cgaactaccg ggtcgcgcgg gcgacgggcg
      661 cgaaaaaacc cgccccgggt ggcctagatg tgcgttttgt gtgtatattt ggcgatggcc
      721 ttgaagggcc agtgaagaag tgtacccgac cgggccggta agtgtggagg ttttgagggc
      781 agatccaggc cgttcgaacg acgttatctt gcatccaata gaatatattt tgcctgacgg
      841 atcaacgccg gagttacccg aaacgggtcc gttgggttgg tatgattgat agcatctttc
      901 cccgctgcgg tttcccttct ttatttccga tttgtataat ttattaatgc ctaatctatt
      961 ttgagatatg ggatgtagca attgtggtac gtcaaaggga ggccaaccgg cgggatgcaa
     1021 gagcaacgga ggatgcagca cgggcagttg taaccggttg aatgtttatg attggctggc
     1081 taatttgccg tttagcgacc cggaaagcgg gtgccgggtg gtcgaagtga gtttcaacca
     1141 gggaagccgc aaagactatt tccggaattc cacgacccag gtcttcgaaa aaggagacct
     1201 cgtggcagtc gaagggataa gcggattcga tgtgggtgag gtcaccctga cgggtgagag
     1261 cgtgcggttg cagctaaaga agaaggggat cgaagagcgg aacccggaca tgaagcggat
     1321 catgcggcgg gccagcgaaa aagacatcga catcatgaga caaaacaagg gccgcgaacg
     1381 ggatgcgctg atccggtccc gggcgatcgc caggcagctg aagctggaca tgaagatgag
     1441 cgaggtggag atccaggccg acggcagaaa agccactttt ttttatatag cggatgaccg
     1501 ggtcgatttc cgcgagctta taaagatata tgccagcgag ttccggctga aggtcgaaat
     1561 gcgacagatc ggcgcccgcc aggaggcggc aaaggtaggc ggaataggaa gctgcgggag
     1621 ggaactttgt tgcgccacct ggctgagcga cttcaagtca gtcaacacca cggccgcccg
     1681 ctatcagaat ctatccatca accaaagcaa gctgagcggg caatgcggcc ggttgaaatg
     1741 ttgcctcaac tacgagctgg atacctatct ggacgccctg cagaattttc ccgacaatgc
     1801 cgagatcctg caggtggcga agggggccgc caatcttatc aagaaggata tctttaaaaa
     1861 cctgatgtgg tatgtactgc ccgacagcaa caagcaatac ccgctgacca tcgaacgggt
     1921 gcggaagatc cggcaaatga accttcaaaa tatggttccc gatacccttg agccggtcga
     1981 agtgagtacc ggcaagccaa aagagacgga agaaacgggc tttgttgacc tggtggggca
     2041 aataagcttg aaaacgctgg aaaaggcgga ccggaaaaaa cggcacgggc accacaaaca
     2101 tggcggtcag ggaaggaagg aaggcttcca gggccgcgac ggaggacaaa gccgggacgg
     2161 tggacagggt aaagagggag gacagggccg ggacggcggc cagggaagag aagcgatcca
     2221 gggtcgcgac ggcggccgcg ggcgtgacgg gcaacgggg
//
LOCUS       DATGKE010000302         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_119752, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000302 DATGKE010000000
VERSION     DATGKE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119752"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VKU83_05155"
     CDS             <1..987
                     /locus_tag="VKU83_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HLI92970.1"
                     /translation="WITHLCAPAFVFLSGTSAWLSLNRRFLLKRGVALLLIEFTIVNF
                     ALSFDIRFRLQIFEVIAAIGTGMILLSFLSRLSTKTILVLALLLIFGHDLVLRIPMPK
                     DPAVSVPGSLLFAPNAFPLGAGRLFVVAYPILPWLGIMLAGFSAGRLFTLPVQQRTRI
                     FVRLGVASLALFVLLRLSNWYGDPFPWSVQRNAVYTFLSFMNVTKYPPSLLFALVTLG
                     ILFLILAAAEAKDNPLTRRLIVYGRVPLFFFIVHLYLIHGLLIAIVLLQGYPWSALDF
                     SPFKFGRPDGAGLSLSQIYLVWACVVVALYPVCSWYGRCKALHKEKRWLRYL"
     gene            complement(1135..1443)
                     /locus_tag="VKU83_05160"
     CDS             complement(1135..1443)
                     /locus_tag="VKU83_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92971.1"
                     /translation="MNDIVAEKLVQKVTNLMDEVTDVNLLVRRIPPPQETINKISDRI
                     DAIHVSVGEIAKGIEADQARIDVLEAPAETLLEGRKKADTAIENALENFRRTGRASES
                     "
     gene            complement(1463..2296)
                     /locus_tag="VKU83_05165"
     CDS             complement(1463..2296)
                     /locus_tag="VKU83_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HLI92972.1"
                     /translation="MARGRLSKGQIKKLRERPLRFYVSFNQQEYDVVVRESRMAGVCR
                     AAYVRAMTFQGQLIPRLSAEELGPFREMVSISNGLNELIKIAREKGSSELLPVFEQYR
                     EAMDTLLNRNRDVVQKLVKDYELIPAGKKNLRQTNFDALDNSETRKYAVFRSIQQCLP
                     GCRDFDELEQRLHEQGVDTRYRVDAQTGQRVGISFRYQEESFKGSEIDRDYSFQRLAA
                     TLGQRQQLSQWESEKLVLGQAQDRQEQLARELAQRQQEELRQQEEFRHTHRQGHKLRV
                     H"
BASE COUNT          433 a          721 c          531 g          638 t
ORIGIN      
        1 tggatcaccc atctttgcgc gccggccttc gtattccttt cgggcacatc ggcctggctc
       61 tcgctgaacc gccgtttcct gctcaaaagg ggagtcgcac tgctgctgat cgaatttacc
      121 atcgtaaatt ttgcgctgag cttcgatatc cggttccggc ttcagatctt cgaagtgatc
      181 gccgccattg ggacgggtat gatcctgctt tcgttcctct cccggctgtc aacaaaaact
      241 atcctcgtgt tagccctgct gttgatcttc ggacacgatc tcgtgctgcg gataccgatg
      301 ccgaaagatc cggccgtgtc ggtccccggc tctctgctat tcgccccgaa tgcttttcca
      361 ctgggggcgg gtcgtctttt cgttgtcgcg tacccgatat tgccctggct ggggatcatg
      421 ctcgcagggt tcagtgccgg acggcttttt acgctgccag tgcagcagcg gacccgcatc
      481 ttcgtccgtc tcggcgtggc cagtctggcg ctttttgttt tgttgcggct aagcaactgg
      541 tatggcgacc ccttcccctg gtcggtgcaa aggaatgcgg tctatacgtt cctgtcattc
      601 atgaatgtca ccaaatatcc cccctccctg cttttcgcgc tcgtcaccct gggcatcctg
      661 ttccttatcc tggcagccgc cgaagcaaag gacaatcccc tcacccggag gctgatcgtc
      721 tatggccggg tgccgctgtt cttttttatc gtccatctgt acctgatcca tgggttgttg
      781 attgccatcg tcctgctgca gggctatccc tggagcgcgc tcgacttcag cccattcaaa
      841 ttcggccgcc ccgacggcgc cggcctgtcc ctcagccaga tctacctggt atgggcttgc
      901 gtcgtcgtcg cgctgtatcc cgtctgtagc tggtacggtc gctgcaaggc gctccacaag
      961 gaaaagcggt ggttgaggta tttataggcc tatttaaata gcgcgattag tattaaccct
     1021 atcagcaaac tgcctgcgag aaccgcgaaa aaaaggaccc gaacagccag gtcgtcttgt
     1081 ctttttgcca ctttgtcgaa cttgaacaag atatcctgct gtacctcgga tactttacga
     1141 ttcacttgct ctgcccgtcc ttctaaaatt ctccaaggca ttttcgatcg cagtatccgc
     1201 tttctttcgc ccctccagaa gtgtttccgc tggagcttcc aatacgtcga tcctggcctg
     1261 atccgcctct atccccttcg cgatctctcc gacactaacg tgtatcgcat cgatacgatc
     1321 actgatctta ttgatcgtct cctggggagg cgggatccgt ctaaccagca aattgacgtc
     1381 cgtcacctca tccatcagat tggtcacctt ctgtaccaat ttctctgcaa ctatatcatt
     1441 cataacttga ttttttattt agttaatgta ccctcaactt atgaccttgc cggtgcgtat
     1501 gccgaaactc ttcctgctgc ctcagctcct cttgctgccg ctgtgcaagc tccctggcca
     1561 gttgttcctg tctatcctgc gcctgtccca ggacgagctt ttcgctttcc cattgagaaa
     1621 gttgctggcg ttggcccagc gttgccgcca accgctgaaa agaataatcc cggtcgatct
     1681 cgcttccctt aaaagactcc tcctgatacc ggaagctgat cccaactcgc tgacccgtct
     1741 gtgcatcgac ccggtatctg gtatccaccc cttgttcatg cagccgttgt tccagctcgt
     1801 caaagtcacg gcaacccggc agacattgct gtatgctgcg aaacaccgca tatttgcggg
     1861 tctccgagtt atccagtgca tcgaaattcg tctgccgcag gttcttcttt cctgcaggga
     1921 tcagctcata atccttgacc agtttttgca ccacatccct gttcctgttt agtaaggtat
     1981 ccatcgcctc gcgatactgt tcaaaaaccg gcaacagctc cgacgatccc ttctcgcgtg
     2041 ctatcttgat cagctcattc aatccatttg aaatgctcac catttctctg aacgggccca
     2101 actcttcggc cgataaccgc gggatcaatt gcccctgaaa ggtcatggcg cgtacataag
     2161 ccgcacggca tacgcctgcc atccgggact cccggacgac cacatcatac tcctgctgat
     2221 taaaagaaac gtaaaaccgg agcggccgct cccgtagctt cttgatctgg ccttttgata
     2281 aacgccccct agccatcttc ctgcaatttt attctcgcaa ggt
//
LOCUS       DATGKE010000303         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_124684, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000303 DATGKE010000000
VERSION     DATGKE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124684"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VKU83_05170"
     CDS             complement(<1..576)
                     /locus_tag="VKU83_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92973.1"
                     /translation="MTTTSFGKKLITCFLAGLVIGAAVFRQGITYFRAWLPFRALAVI
                     PSAAAVAGIIYAIIWQARRTNNPATLAFWQGLIRYGVAYDLAEFGWSKICHRQFVMPG
                     SMLDLPYNRFSPADLFWTFFSHSYLFGCIIGGLEIVGAMLLLFRRTRLAGVFVLLPIL
                     ANILLMDIFYDVGAQEHAAIMMAGALYFFFIE"
     gene            786..1196
                     /locus_tag="VKU83_05175"
     CDS             786..1196
                     /locus_tag="VKU83_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI92974.1"
                     /translation="MTLARDNTEYKTCLLVDDDLDDQEIFAMALGQVNAAIRCEFAND
                     GTEAMEKLQNKNVSPPDYIFLDLNMPRMDGRQCLIEIKRQSHLRHIPLVVYSTSSDED
                     FQAEMIRLGATACVVKPPLMSMLKKKLMDFFSQY"
     gene            1204..4632
                     /locus_tag="VKU83_05180"
     CDS             1204..4632
                     /locus_tag="VKU83_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI92975.1"
                     /translation="MPNKPMDTEQFLAEGGEMGSLIRAFDWTTTSLGIPSSWPLSLKT
                     SIAIMLRSGYTIFVWWGPEMIVFYNDAYRQVLGKKHPLALGKPASLVWSEIWGQIGPM
                     MNQVLNEGRQIYAEDLLFLIERRGFIEESYFTFSYSPIPHDEGGVGGIFCACNEETAK
                     ILRQRRLKTLNDLSAATRDTRTVGDVFDVCTPILAENSKDIPFSLLYRFQPAAGEARL
                     AGHTGFSDDRHRALEPVIKLNETGAFGWPLKLAADAAKPVTIENVRNLFDPLPAGPWD
                     SGPEKAVIIPIAQSGQEPYGFLVAGISPKLEFDDEYRGYLDLIAGQLSSAIASVQAFE
                     AERQRAEALADIDRAKTAFFSNISHEFRTPLTLMLGPLEELISRDAADFSPAALQGLQ
                     STHRNAMRLLRLVNTLLDFSRIEAGRIQAQYYPVEIGGFTRDLASAFRSLIEKAGLDF
                     IVDCTEPSDPVYVDREMWEKIVLNLLSNAYKYTLAGKITVTLRQEGDRVLLRVSDTGV
                     GIPEKELPRMFERFHRVKTSVGRTHEGTGIGLSLVNELVKLHKGGIYIESKEGVGTSF
                     TVSIPVGRSHLPPEQIFSGTDGKYDPLIRDLYISEVASLSGEEARSLGNGLLHEMATE
                     TRGERVLVVDDNGDMRTYISTLLSKSYLVETAINGRDALEKLQLGTPNLIISDIMMPV
                     MDGMQLLDAVKNNPETAAVPVILLSARAGEEAKIEGYNVGADDYLVKPFSAKELLARV
                     QAQIKTARIRAMAEAHLRNLFMQAPVAIAIYRGPEMVIELANEKVLEFWGRSAEQVLN
                     KPLFEALPEISKEFFEPIIMRVYNTGERFFGPEIPATLKRNGKIETVYVNFILEALRD
                     VDGKVTSVMVLANEVTDLVNARRMAENNAAELEKRVAERTVELAQSNLKLVNANKELE
                     QIAFIASHDLQEPLRKIQTFADLLRENLGNPASAERYIEKIISSGSRMMLLIKSVLNY
                     ARLSRQMVEYERVDLNEVMEEVKTDFELPIAETKAVIRNNGLPVVKGNRLQLSQLFAN
                     LLSNSLKFAVTNPEIVIGSASFELEQPEEFPSLEAGRKYVEIVFRDNGIGFEPKYAEQ
                     IFSMFRRLTKEYGGTGIGLALCKKIVDNHRGAIVAKSEVGKGTAFSVILPFDDL"
     gene            4759..5376
                     /locus_tag="VKU83_05185"
     CDS             4759..5376
                     /locus_tag="VKU83_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLI92976.1"
                     /translation="MPRTSSDTRTSILQVALQLFLQKGYKAVSYENITRKTRLSKGAI
                     YHYFPSKQDLLVQTFEFLLEAARQRDTAALVAEVRDIPSFKRMYLRMKARQLKSFYSR
                     LNQKQLQFNKVLFFIEAINEYHRLGEIVADLLRQEVDFLRRCMEELKKHRLIPAGKDP
                     ESLAQGLFWMLDGAENMLYIRKSRDQARDYLALYKKTLDLFFNLI"
     gene            5378..>5880
                     /locus_tag="VKU83_05190"
     CDS             5378..>5880
                     /locus_tag="VKU83_05190"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HLI92977.1"
                     /translation="MKSAIQRSIGLLSLLLAAPAAYSQTAKEIVARAEDNIHGLYAKT
                     EMTIQIIRPAWTRTMNLKTWSRGDAYSLMLITAPAKDAGTVFLKRGKEIWNWLPSIER
                     SVKLPPSMMSQSWMGTDFTNDDLVKASSRVDDYTHKIVGDSVIEGRSCWKIEMIPLPA
                     ASVVWGKV"
BASE COUNT         1526 a         1455 c         1648 g         1251 t
ORIGIN      
        1 ttcaataaag aaaaaataca aggcccccgc catcataata gccgcatgtt cctgcgcgcc
       61 gacgtcgtaa aagatgtcca tcaacaggat gttcgcgagg atgggcagca ggacaaatac
      121 gccggcgagc cgggtccggc gaaaaagcag cagcattgcc ccaactatct caagcccgcc
      181 gatgatacag ccaaacaggt acgaatgact gaaaaaggtc cagaacaaat cagccgggga
      241 aaaccggttg tacggcaggt ccagcatact cccgggcatc acaaactgcc ggtgacagat
      301 cttcgaccag ccgaattccg caagatcgta tgccacgcca tagcgaatga gcccttgcca
      361 aaaagctagg gtggccgggt tgtttgtcct tcgggcctgc cagataatgg cataaatgat
      421 cccggcaacc gctgcggccg acgggatgac cgccagcgcc cggaaaggca accaggcccg
      481 gaaataggta atcccctgcc gaaaaacagc ggcgccgatc accaacccag ccaggaagca
      541 ggtgatcaat ttcttaccaa aggaggttgt ggtcatgcgg gatggacggc gtatagtgac
      601 tatttggaaa gagactatga aaataacgta tcggcaagac atttcgggcg atcctaacgg
      661 tgacgcaaaa ggcccgtaag gaccgcgata tgtttcgcat cctaaaaaga ttgataacta
      721 gccttttagg gagcggtctc agggaattgg ggaaaggttg atatttttgc tataaataag
      781 atttcgtgac ccttgctcgt gacaatacgg aatacaagac atgcttactt gtggacgacg
      841 atttggatga ccaggagata tttgcaatgg ccttaggtca ggtaaacgcc gcaatccggt
      901 gcgagtttgc caatgacggg acagaggcaa tggaaaaact gcagaataaa aatgtctccc
      961 cgccggatta tattttcctt gatctgaata tgccgcggat ggatggccgg caatgtctta
     1021 tcgagatcaa gcggcaatcc catctcaggc acatacccct cgttgtttat tccacttcat
     1081 cggatgaaga ctttcaggcg gagatgataa gactgggtgc gacggcgtgt gtcgtaaaac
     1141 ctcccctgat gtccatgctc aaaaagaagc ttatggactt tttcagccaa tattgaataa
     1201 tgtatgccga ataaacccat ggatacggag caatttcttg cggagggcgg agaaatggga
     1261 tcactgatcc gggcatttga ctggacgaca acttctctcg gaattccttc cagctggccg
     1321 ctcagcctta agacatccat cgctatcatg ttgcggtccg ggtatacgat ttttgtctgg
     1381 tgggggccgg agatgatcgt gttttataat gacgcttaca ggcaggtgtt gggaaagaag
     1441 cacccgcttg ctttgggaaa gccggcgagc ctggtatggt ccgaaatctg gggtcagata
     1501 ggacccatga tgaaccaggt cctgaatgaa ggccggcaaa tttatgcaga ggatctctta
     1561 ttccttatag aacgaagagg gtttattgaa gaatcctact ttaccttttc ttatagtccg
     1621 atcccccatg acgaaggcgg cgttggcggc atcttttgcg cctgcaatga agagacagca
     1681 aaaatattga ggcaaaggag actgaaaacg ttgaatgacc tgtccgcggc tacaagggac
     1741 acaaggaccg tgggcgatgt cttcgatgtc tgtactccaa tcctggcgga aaactccaaa
     1801 gatatcccat tttcgctgct ttatcggttc cagccagctg caggcgaagc caggctggcc
     1861 gggcatacgg gcttttcgga tgaccggcac cgtgcgctcg agccggtcat taagctgaat
     1921 gagacggggg ctttcggctg gccattgaaa ctggcggcgg acgccgccaa acctgtcacg
     1981 atagaaaatg tcaggaacct gttcgacccg ctgccggctg gcccatggga ctcagggccg
     2041 gaaaaggcgg tcattatccc gatcgcgcaa tcggggcagg agccgtatgg attccttgtt
     2101 gccggcatca gccctaaact cgaattcgat gacgaatatc gcggctacct ggatctgatc
     2161 gccggccagc tctcatctgc tattgccagc gtccaggcgt ttgaggcgga acggcagcgg
     2221 gccgaggcgc tggccgatat cgatcgcgcc aaaacagcgt ttttcagcaa tatcagtcat
     2281 gaatttcgta cccctttgac cctgatgttg gggcccttgg aggagctgat cagccgggac
     2341 gcggccgatt tttcaccggc agccttgcaa ggcctccagt ctacgcatcg caacgccatg
     2401 cgtctcctga ggctggtgaa caccttactc gatttcagcc ggatcgaagc cggccggata
     2461 caagcgcagt actatccggt ggaaatcggc ggtttcacca gagatctggc aagcgccttc
     2521 cgatccctta ttgaaaaagc cggcctggat ttcatcgtcg attgcacgga gccttcggat
     2581 ccggtttatg tagaccggga aatgtgggaa aagatcgtcc tgaatctttt atcgaatgca
     2641 tacaaatata ccctggccgg taaaataacg gtgacgttgc ggcaggaggg agaccgcgta
     2701 ttgttacggg tgagcgacac gggagtggga atcccggaaa aggaactgcc ccggatgttc
     2761 gaacggttcc atcgggtaaa aacttcggtc ggccgcaccc acgaaggtac aggaatcggc
     2821 ctgtcgctgg tgaatgagct ggtgaagctg cacaaaggag gaatttatat cgaaagcaag
     2881 gaaggggtgg gaactagttt taccgtatcc atacctgtcg gaaggagcca cctgcctccg
     2941 gaacagatat tcagcggaac agatggtaaa tatgaccccc tgataagaga tctttatatc
     3001 agtgaggtgg ccagcctatc gggggaggag gcacgaagcc tggggaacgg cctgctgcac
     3061 gagatggcca ctgaaaccag aggggaacgg gtactggtcg tcgacgataa tggggacatg
     3121 cgaacataca taagcacgct cctcagcaag agctacctcg tcgaaacagc gatcaacggc
     3181 agggatgccc ttgaaaagct tcaactcggg actcccaatc tcatcattag cgacattatg
     3241 atgccggtga tggacggcat gcagctcctg gatgcagtaa aaaacaatcc tgaaacggcc
     3301 gctgtcccgg tgatccttct atcggccagg gcgggcgaag aggcaaagat agagggatac
     3361 aatgtagggg cagatgacta tctggtaaag ccgttctcag ccaaggagtt gctggcaagg
     3421 gtgcaagcgc agatcaagac agccaggatc cgcgcgatgg cggaggcaca cttacgcaac
     3481 ttgttcatgc aggcgccggt ggctatcgcg atataccggg gaccagaaat ggtgatcgag
     3541 ctggccaatg aaaaggttct tgaattttgg ggcaggtccg cggaacaggt attgaacaag
     3601 ccgctgttcg aagcattgcc cgaaataagc aaggagtttt ttgaaccgat catcatgcgg
     3661 gtgtataata ccggggaaag gttttttggt cctgagatac cggccacgct gaaacgaaac
     3721 ggaaagatcg agaccgtata tgtcaatttt atcctggaag ctttacgcga tgtcgacgga
     3781 aaggttacca gcgtgatggt gctagccaac gaggtcaccg acctggtgaa tgcccgccgg
     3841 atggcggaaa acaatgcggc agagctggaa aaaagggtgg cggaaagaac tgtcgaactt
     3901 gcacagtcga atctcaagct ggtgaacgcg aacaaggaac tggaacagat tgcctttatt
     3961 gccagccacg acctgcagga gccgttgcgt aagatacaaa cgttcgccga cctgctcagg
     4021 gaaaaccttg gaaacccggc aagtgccgaa aggtatattg aaaagatcat ttcttccggc
     4081 agccgcatga tgctgctgat caaatcggtg ttaaactacg cacgattatc gaggcagatg
     4141 gttgaatatg aacgtgtcga tctgaatgag gttatggaag aggtcaagac ggatttcgag
     4201 ctgcctatag ccgaaacaaa agcagttatt cggaacaacg ggctccccgt ggtcaaaggc
     4261 aaccggcttc agttgagtca actctttgcg aatctgctga gcaattcgtt gaaatttgcg
     4321 gtcacgaatc ctgaaatcgt catcggttcc gcttctttcg agttggagca acccgaagaa
     4381 ttcccgtctc ttgaggccgg caggaaatac gtcgaaatcg tctttcggga taatggcatc
     4441 ggatttgagc caaagtatgc cgagcagata ttttcgatgt tccggcgtct gaccaaagag
     4501 tacggtggaa ccggtattgg ccttgcactc tgcaagaaga tcgtagacaa tcatagggga
     4561 gcgattgtgg cgaagagtga ggtgggcaaa ggaacggcat ttagtgtgat cttgccgttt
     4621 gacgatttat gacattcagg cgggacgcca tagcaatgga ctcccacggc gacgcccgct
     4681 gggataattg cgtcgcggtt aaaaaaattt cctatcttac ataccgaatg gtcggtttgt
     4741 gatcatttcg cgacaactat gcccagaaca tcttccgaca ccaggacatc cattcttcag
     4801 gtggcattgc agcttttcct gcaaaaaggc tacaaggccg tgtcgtatga gaacatcacc
     4861 cggaaaacgc gcctctccaa aggagcgatt tatcattatt ttccctccaa gcaggacctg
     4921 ctggtgcaga cattcgagtt cctcctggaa gcagccaggc aaagggacac ggctgcgctg
     4981 gtcgcggaag taagggatat cccctccttt aagcgcatgt acctgcgcat gaaggccagg
     5041 caattgaaaa gtttctatag ccgtttgaat caaaagcaac tacaattcaa caaggtgctg
     5101 ttcttcatcg aggccatcaa cgaatatcac cgtcttgggg aaatcgtcgc cgacctgctg
     5161 cgacaggagg tggatttcct gcgccggtgc atggaggaac tgaaaaagca tcgactgatc
     5221 cctgccggca aggaccccga atccctggcc caggggctgt tctggatgct ggatggcgct
     5281 gagaacatgc tctatatccg caaaagccgg gatcaggcac gggattacct ggcgctctat
     5341 aaaaaaacgc ttgacctgtt ctttaatctt atctgatatg aaatcggcca tccaacgctc
     5401 aatcggactt ctatccctcc tcctcgccgc tccggcggca tacagccaga cggccaaaga
     5461 gatcgtcgcg agggcggagg ataacattca tgggctctac gccaaaacag agatgaccat
     5521 ccagatcatc cggcctgcct ggacgaggac catgaacctg aagacctggt cgagaggcga
     5581 tgcgtattcc ctgatgttga tcacggcccc cgccaaggat gccggcaccg tcttcctcaa
     5641 gcgggggaag gagatctgga actggcttcc cagcattgaa agatcggtca agctaccccc
     5701 ttccatgatg tcgcaaagct ggatggggac ggattttacc aacgacgacc tggtgaaggc
     5761 ttcctcccgg gtggatgatt atacgcacaa aatcgtaggc gacagcgtga tagagggccg
     5821 gtcttgctgg aagatcgaaa tgatccccct gccagccgcc tccgtggtat ggggcaaggt
//
LOCUS       DATGKE010000304         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1148321, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000304 DATGKE010000000
VERSION     DATGKE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148321"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VKU83_05195"
     CDS             <1..376
                     /locus_tag="VKU83_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI92978.1"
                     /translation="HTRIFVLDEATANLDFATEEEVRKTIDKIRHNATVIVIAHRYSM
                     IREADHVIVLSEGRVLEAGRPEELLWNGGWFAQFAAAAEQGLAAGDDVPSEEPDGSAD
                     EDIAGQGDGEETDEDADDADGP"
     gene            452..1483
                     /locus_tag="VKU83_05200"
     CDS             452..1483
                     /locus_tag="VKU83_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLI92979.1"
                     /translation="MPKSILPLLLLSLLCSSEQAAASAGPDSLPVPCREKAEQLLDQA
                     LGFMEKNYYRRQEVDWTTLAAHARQQLQYADNCDDAYASISWCFRQLGERHSFIMPPD
                     KAARYTGEGSAGTGGGLPDVVGEIRGEWLTDSIGYLTIPWVSTTDSLLCERIADSVQS
                     VIAHLDTRRLSRWIIDLRHNTGGNCWPMLAGIGPLLGDGIYGYFVASGEKIPFSYYDG
                     SAFQGKRVLCRVSNRHGYHASSGHNSIVVLTGNRTVSAGEIVALAFKGREHVRLIGQP
                     TAGLTTANATYSLSDRSMLVLTVCQEADCVGRICDGRIQPDEYISAGNTVTDADPARA
                     AAIAWLENE"
     gene            1531..3189
                     /locus_tag="VKU83_05205"
     CDS             1531..3189
                     /locus_tag="VKU83_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HLI92980.1"
                     /translation="MRLSVYRTFLYSAIPLSLSSCASKGGNTDAGLASFNKDSLAAAI
                     RVLASDSFQGRRPFTEGESRTVDYLVKSYKALGLEPGNGTGYTQDVPLVEISPSIAPS
                     LRLESSKGKMDFRNIDDFVLWTERPDSINIIDRNDVVFAGFGIVAPEYHWNDYAGLDV
                     KGKTVLVMVNDPGFYDSTLFKGHTMTYYGRWTYKYEEAARQGAKACLIIHNTAAASYP
                     FQVVQSSNGGVKLHLDNRKIQGNQLTVQGWITEETAAKMLAAGGKDSNLLNSAKKPGF
                     KAIPLPIRISAAVKVREVYNMSHNVIAKISGTKYPDEYVIYSAHWDHLGIGKPDAKGD
                     SIYNGAADNASGTAALLQIARAFESLPAKPERTVIFLSVTAEEQGLLGSAWYAEHPVY
                     PLAKTVADLNIDVLNTYGPTRDITYSGKGQSDLEDSLAAAAKTVGRYVAPEDHPEAGH
                     YFRSDHFSFARAGVPSITADGGVDDLSRGIVYGKKKHDEYTATRYHQPADQIDSTWDL
                     DGGLQDVELVYTIGERLANSHSWPEWKAGSEFKAVRDKTAAERK"
     gene            3257..5005
                     /locus_tag="VKU83_05210"
     CDS             3257..5005
                     /locus_tag="VKU83_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLI92981.1"
                     /translation="MGDLQIKKQPKQYDAVIVGSGAGGGMAAYVLAHAGLKVCLLEAG
                     PMFDPRKDSNQLRNAWESPRRGASTRFRPFGDFDGCYWGWEVDGEPYTQTGKKGTIAW
                     EWWRARMLGGRTNHWGRIALRFGPKDFKRKSIDGLGDDWPIGYEDVKPYYDKIDKLLG
                     VFGTNEGLPNDPDGFFLPPPKPRLHELMIKNAGAKVGVPVIPSRLAILTKSINSERGQ
                     CFYCAQCGRSCKVYADFSSSSCLVIPALKTGNVDLVTNAMAREVLTNKEGLATGVSYI
                     NKDDNQEYQVSGRTVVLAASACESARLLLNSKSARYPNGLANGSGVVGKYLHDSTGAD
                     MGGIIPALFDRKRYNEDGVGGMHVYSPWWLDNKKLDFPRGYHIEFGGGFGQPLYGFNW
                     DIQNLNGDYKIKGKQKEAGGYGASLKEDYRYFFGAHVGMAGRGEAIARIDNYCEIDPD
                     VVDKYGIPVLRFNTSHSEYEIKQARHMKKTFKEILLAMGAEKIWGDEDGEHNNWGLHA
                     PGNIIHEAGTVRMGNDARTAPLNKWSQAHEVKNLFCVDGGPFVSQADKNITWTILALA
                     MRSSEYMVDEMKKRNI"
     gene            5016..>5203
                     /locus_tag="VKU83_05215"
     CDS             5016..>5203
                     /locus_tag="VKU83_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92982.1"
                     /translation="MDRRKSIKTILIGTVSAGALAAEACHTSEKNPAASTGADTTAEG
                     KAAASLNPSGYNRMKEET"
BASE COUNT         1227 a         1484 c         1474 g         1018 t
ORIGIN      
        1 ccacacccgc atcttcgtgc tcgacgaagc taccgccaac ctggactttg ccacggaaga
       61 agaggtcagg aagaccatcg ataagatccg tcacaacgca acggtgatcg tcatcgccca
      121 tcgttattcg atgatccgcg aagcggacca tgtcattgtc ctgtcggaag gccgggtgct
      181 cgaagccggt agaccggagg agcttctttg gaacggaggc tggttcgcac aatttgccgc
      241 cgccgccgaa caaggcctcg cggccgggga cgacgtaccc tctgaagagc cggatggttc
      301 cgcggacgaa gatattgccg gccaggggga tggcgaagaa accgatgaag atgcggacga
      361 cgctgacggt ccctgaaaat taacccgctg aaagtttatg gaatccggcg cttttaatct
      421 ccttcttagc tttgacccaa tcattttgcc gatgcccaag tcaattttgc cgttactttt
      481 actctccctg ctttgcagca gcgaacaagc cgcggcttcg gccggccctg actccctgcc
      541 cgtcccctgt cgcgaaaagg ccgaacagct gctcgaccag gccctgggat tcatggaaaa
      601 gaactattac cgccggcaag aggtcgactg gacgaccctt gccgcccacg cccgccaaca
      661 gcttcaatat gcagacaact gcgacgacgc ttacgcctcg atcagctggt gcttccggca
      721 gcttggcgaa cgtcacagct tcatcatgcc gccggataag gcggcgcgct ataccgggga
      781 aggctctgcc ggaaccggcg gaggccttcc tgatgtcgtc ggtgagatca gaggcgaatg
      841 gctgacagac agcattggct acctgacgat accctgggtc agcaccaccg acagtctctt
      901 gtgcgaacgg atcgcggaca gtgtgcagtc ggtgatcgcc catttggaca cccggcgcct
      961 gtcgcgatgg atcatcgatc tgcgccacaa caccggcggc aactgctggc ccatgcttgc
     1021 cggcatcggc ccactgctcg gcgacggcat ctatgggtac tttgtggcct caggagaaaa
     1081 aataccgttt tcgtattacg acggctcggc ttttcaggga aaacgtgttc tatgccgggt
     1141 cagtaaccgg cacggttacc acgcgtcttc cggacacaat tccatcgttg tgctgaccgg
     1201 taacaggacc gtcagcgccg gcgagattgt ggctctggca ttcaaaggcc gtgagcatgt
     1261 acgactcatt ggccagccta ctgccggtct caccaccgcc aatgccacgt attccctttc
     1321 cgaccggtcg atgctcgtgc tcaccgtatg ccaggaagcc gactgcgtcg gccggatctg
     1381 cgacggtcgc attcagccgg atgaatatat ttccgcaggg aacacggtga ccgatgccga
     1441 cccggcgcgc gccgctgcca tcgcctggct ggaaaacgaa tgagacactt tccctatctt
     1501 tagcccctcg cagcacaaaa tcgactgctt atgcgccttt ctgtttaccg gaccttcctc
     1561 tattcagcga tcccgctctc cctaagctcc tgcgcatcca aagggggaaa cacggatgcc
     1621 ggcctggcca gcttcaacaa ggacagcctg gcggcagcga tccgcgtcct tgccagcgat
     1681 tccttccagg gaaggcggcc gttcaccgaa ggagaatccc gcaccgttga ctatctcgtc
     1741 aaatcttata aggcgcttgg cctggaacct ggtaacggta ccgggtacac gcaggatgtg
     1801 ccgctggttg agatctcccc ttccatcgcc ccaagcctgc ggttggaatc ttcaaaggga
     1861 aagatggatt tcaggaacat cgacgacttc gtcctctgga cggaaaggcc cgattctatc
     1921 aatatcatcg acaggaacga cgtggtcttt gcgggattcg gcatcgtcgc cccggaatat
     1981 cattggaacg attatgcggg attggacgtg aaaggcaaga ctgtcctcgt catggtgaac
     2041 gacccgggat tttacgactc tacccttttc aagggccaca ccatgaccta ttatggccgc
     2101 tggacctaca aatatgagga agctgcaaga cagggagcaa aagcctgcct catcatccac
     2161 aatacagcgg ccgcttctta tccgttccag gtagtccaaa gttctaacgg cggggtgaag
     2221 ctccatcttg acaaccggaa gatccaggga aaccagctga ctgtccaggg ttggataacc
     2281 gaagaaaccg cggcaaaaat gcttgcggct ggcggaaagg acagcaatct tctaaactcc
     2341 gcgaaaaagc ccggattcaa agcgatcccg ttgcctatac ggatatcagc cgccgttaaa
     2401 gtgagggagg tctacaatat gtcgcacaac gtaatcgcca aaataagcgg aaccaaatat
     2461 ccggacgaat acgttatcta ctccgctcac tgggaccacc tcgggatcgg gaagccggac
     2521 gcgaaaggtg attccattta taatggtgct gccgacaacg ccagcggcac cgccgcgctg
     2581 ctgcaaattg cccgggcgtt cgaaagtcta cctgcgaaac cggagcggac ggttattttt
     2641 ctatcggtca ccgccgagga acagggcctg ctgggatcgg cctggtacgc ggaacatccg
     2701 gtctatcccc tcgctaaaac cgttgcggat ctgaatatcg acgtgttgaa cacctatggc
     2761 cccacaaggg atatcacgta tagcggaaaa ggacaatctg acctggagga ttcgctcgcg
     2821 gccgctgcca aaacggtcgg ccggtacgtc gcgccggaag atcatccgga agccggccat
     2881 tattttcgct ctgaccactt cagctttgcc cgtgccggcg tgccgtcgat tacggctgat
     2941 ggaggtgtcg acgatctcag ccgcggcatc gtttacggca agaagaaaca cgacgaatac
     3001 acggctaccc ggtaccatca acccgcagat cagatcgatt ccacgtggga cctggacgga
     3061 ggtcttcagg atgtcgaact ggtctatacc atcggcgagc ggcttgccaa cagtcacagc
     3121 tggccggaat ggaaggccgg ttctgagttc aaggctgttc gcgacaagac cgcagcagaa
     3181 agaaaataaa aaaataggcc gctatatggc taaaccttta aatttacgct ttatttttct
     3241 ttaagaccgt acaagcatgg gagacctcca gatcaagaaa cagccaaaac aatacgacgc
     3301 agtaatagtc ggctcgggcg cgggtggcgg aatggcagcc tatgtgctcg cccacgcagg
     3361 gttaaaagta tgccttctcg aagcggggcc aatgttcgat ccgcgaaagg actcgaatca
     3421 gttgcggaac gcctgggaat cgccgcgccg cggcgccagc acaaggtttc ggcccttcgg
     3481 cgattttgac ggctgctatt ggggctggga agtagatggc gaaccctaca cccagaccgg
     3541 caaaaaggga acgatcgcgt gggaatggtg gcgggcccgc atgctcggcg gacgtaccaa
     3601 ccactgggga cggattgcgt tgcggttcgg tccgaaggac ttcaaacgta agagcatcga
     3661 cggcctgggc gacgactggc ctattggcta cgaagatgtg aagccatatt atgacaagat
     3721 cgataagctg ctgggtgtat ttggaacgaa tgaagggttg ccgaacgatc cggacgggtt
     3781 ctttcttccg ccgcccaagc cgcggctgca cgagttgatg atcaagaacg cgggcgcgaa
     3841 ggtcggcgta ccggtcatcc cttcccggct ggccattctg accaagtcga tcaacagtga
     3901 acgcgggcag tgcttctatt gtgcccaatg cggccggagc tgcaaagtct acgccgattt
     3961 ctcgtcctcc tcctgcctcg tcattccggc gctgaagacg ggcaacgtcg atctcgttac
     4021 gaatgccatg gcgcgcgagg tattgacgaa taaggagggc cttgcgacag gtgtctccta
     4081 cataaataag gatgataacc aggaatacca ggtctccggc aggacggtag tcctcgccgc
     4141 cagcgcctgc gaaagcgcac gcctcctgct caactcgaag tccgcccgct atccaaacgg
     4201 cctcgccaat ggcagcggcg tagtgggcaa atatctgcac gactctacag gcgccgacat
     4261 gggcggcatc atccccgcac tcttcgaccg caaacgatac aacgaagacg gcgtcggcgg
     4321 catgcacgtt tactcgccat ggtggttgga taacaaaaaa ctggattttc cgcgcggcta
     4381 tcacatcgaa ttcggcggcg gtttcggcca gcctttatat ggtttcaact gggacatcca
     4441 aaatctcaac ggcgactaca agatcaaggg gaaacaaaag gaagccgggg ggtatggcgc
     4501 ctcgttgaag gaagactacc gctatttctt cggtgcgcat gtcggcatgg ccggccgcgg
     4561 cgaagccatt gccaggatcg acaactattg cgagatcgat ccggacgtcg tggacaagta
     4621 cggaattccg gtgctgcgtt ttaataccag ccactcggaa tatgagatca agcaggcgcg
     4681 gcatatgaag aagaccttta aagagatcct tcttgccatg ggcgcggaaa agatctgggg
     4741 cgatgaagac ggcgagcata acaactgggg cttgcatgcg ccggggaata tcattcatga
     4801 agcgggcacc gtcaggatgg gtaacgatgc ccggaccgcg ccactcaaca aatggagcca
     4861 ggcgcacgag gtcaagaatc tgttttgcgt cgatgggggc cccttcgtct cgcaggccga
     4921 caagaatatc acgtggacca ttctggcgct ggcgatgcgg tcgagcgaat acatggtcga
     4981 tgaaatgaaa aaacgtaaca tctagcaata ttccgatgga cagaagaaaa agtataaaga
     5041 caatcctcat tggaaccgtt tccgccggcg cgctcgcggc cgaagcctgc catacgagtg
     5101 aaaaaaatcc cgccgcttcg accggcgccg acacgaccgc ggaagggaaa gccgccgctt
     5161 cgcttaaccc aagcggttat aaccggatga aggaggagac cga
//
LOCUS       DATGKE010000305         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_345064, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000305 DATGKE010000000
VERSION     DATGKE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345064"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VKU83_05220"
     CDS             complement(<1..1951)
                     /locus_tag="VKU83_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92983.1"
                     /translation="MIRNILRTAFRTLSKNRATTLLNIFGLAAGMTSAILIFLWVQNE
                     LSFDRYHPDSNRIYRITAHITSAKWTWATAPLALAQPIRANVPAVESLAAMEPDYAPT
                     FRLGNELVTEKNCAYVDSGWFSVFHYDFLKGSAAEFFRHPFSLILTQTKAKQYFGDKD
                     PIGRTIHIDTLDYRVAAIVKDIPANSSIQSDILIPVDAYLSNPDNRKNALEGGNFNYF
                     IFLKLRPGAQPAKVVATITGIMNKTGSDSTNTLSLTPLKEIHFETGLTYDAGGLQHGN
                     RNTVYIFTVLGVFLLVIACINYVNLTTARASLRAKEVGVRKMIGAGNRSLFGQFIIES
                     LVVSALSLMITVVLVSFLMPAFRDLTARNFSTPLSSAAAWKIIGLTLLAATLLNGIYP
                     ALVLSSFKPLNALKGTALLRFKDVYLRRSLVVLQFSFSVILVAATIIIQRQLYFIQHL
                     DPGYDRSQVFSFRMPWQSQSHVVTVKEQLQSYADISGVAVGSDGSVVQINSSNSGSAD
                     WDGHDSSFRPTVFQANVDEDYASVLQLKMAQGRWFDRRYPTDKHNFIVNETAVNEFVN
                     LRKPAVGQRFSFQGDTGQIIGVVKDFHFASLHEKIRPLVFFDRNDWRTTLFVKASPGK
                     ATSTLAEARAIWQRYVPGKPFDYTFL"
     gene            complement(2108..>3185)
                     /locus_tag="VKU83_05225"
     CDS             complement(2108..>3185)
                     /locus_tag="VKU83_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92984.1"
                     /translation="VFIISTIIIYRQLTFVHNRDLGFEKEGLLYMNLQGDLGHHFAAV
                     RTDLLHTGYVKDATLSSSTILQLGSNTGDFEWPGKDPDKQVLITVENASPEYLSTMGV
                     TLAAGRNFHDVAAQDSNNIIINEALAHIITKGNPVGSLITRENGKEKWRVIGVIKDFV
                     YDNMYQSAAPLILFCDPANTNVMTVRLKAGADIAKAVAAIGTVMKAANPAYPFEYHFL
                     NEDFENQFQTETLTGRLSGVFAALAIFISCLGLFGLAGYAAERRTKEIGVRKVLGASV
                     LLLTSLLSRDFILLVLFACLIAFPVAGVLMHHWLSSYAYRTGIDWTVFMLSGAGAIAI
                     ALLTVSYITIRASLANPIRSLRTE"
BASE COUNT          708 a          780 c          991 g          706 t
ORIGIN      
        1 caaggaacgt gtagtcgaag ggcttgccgg gcacatagcg ctgccagatg gcgcgcgctt
       61 cggccagggt gctggtggct ttccccggac ttgcctttac aaagagggtc gttcgccagt
      121 cattccgatc gaaaaaaacg aggggcctga tcttctcatg caagctggcg aaatgaaaat
      181 cctttaccac cccgatgatc tgaccggtat ccccctggaa agaaaatcgc tggccgacgg
      241 cgggtttgcg caggttgaca aattcattta ccgcggtttc attgacaata aaattgtgct
      301 tgtctgtcgg gtagcggcgg tcgaaccagc ggccctgcgc catcttcaac tggaggacgc
      361 tcgcatagtc ttcgtcgacg tttgcctgaa aaacggtggg ccggaagctg ctgtcgtggc
      421 catcccaatc ggccgagccg gagttggaac tgttgatctg gacgaccgaa ccgtcgctgc
      481 ccacggcaac tcccgagata tcggcatacg attgcaactg ttccttgacg gtaacgacat
      541 gcgactggct ttgccagggc atccggaatg agaagacctg gctgcggtcg tagccgggat
      601 caagatgctg tataaagtag agttggcgct ggatgatgat ggtggcggcg accaggatga
      661 ccgaaaacga gaactggagc accacgagac tccttcgaag gtagacgtcc ttgaatcgaa
      721 ggagtgcggt ccctttgagc gcattcaggg gcttaaagga agaaagaacc aacgcgggat
      781 agatgccatt gagcagcgtg gccgccaaga gggtgagacc gatgatcttc caggcagcgg
      841 ctgatgatag cggggtggaa aagtttcttg ccgtaaggtc gcgaaacgca ggcatcagga
      901 aactgaccag gacaaccgtg atcatcagtg aaagcgcact gacgaccagg gattcgatga
      961 tgaattgacc aaacaggctt ctgttaccgg cgccgatcat tttcctcacg ccgacttctt
     1021 tcgcgcggag gctggccctg gcagtggtca ggttcacata attgatgcag gcgatgacga
     1081 gcaggaagac tcccaggacg gtaaatatat agaccgtatt gcggttcccg tgttgcaggc
     1141 cgccggcgtc gtaggtcagg ccggtctcga aatggatttc cttaagagga gtaaggctta
     1201 gggtattggt gctatcactc ccggttttgt tcatgatccc cgtgatggta gcgacaactt
     1261 tggcaggttg ggcgccgggg cgcagcttca ggaaaataaa ataattgaaa ttgccgcctt
     1321 ccaaagcatt tttcctgtta tccgggttgg agaggtaggc atcgacggga atcaggatgt
     1381 ccgactgaat gctggaattg gcgggaatgt cctttacgat agcggccacc cgatagtcaa
     1441 gcgtgtcgat atgaatagtg cgtccgatgg ggtctttgtc accgaaatat tgttttgcct
     1501 tggtttgggt aaggataagg ctgaatggat ggcggaaaaa ctcagcggcg ctgcctttta
     1561 gaaaatcata atgaaaaacc gagaaccagc cgctgtcgac ataggcgcag tttttttccg
     1621 taaccagttc attgcccagg cgaaaggttg gagcatagtc gggttccatg gcggcaagcg
     1681 attcgaccgc cggtacattc gcgcggatag gttgggcaag ggcgaggggt gcagtggccc
     1741 aggtccactt tgcgctggtg atgtgcgcag tgatccggta gatgcggttc gaatcgggat
     1801 gatagcggtc gaaactaagc tcgttctgca cccagaggaa tatgaggatg gcggaggtca
     1861 tgccggcggc gagaccgaag atgttcagca gcgtcgtggc gcggttttta ctcaacgttc
     1921 gaaaggcggt gcgaaggatg ttcctgatca tagctttatt tattcattgc gtcgcgccgg
     1981 gcgaccttga gatgattgcg cagcattttc agcaatttta tatttcattc ggttgaccgc
     2041 ggtccgggac cgctcctgtc tatgcggccc tcgcgtgacc gtgagcgaac gctgggcgcc
     2101 cggggactca ctcggttctc aggctacgga tgggattggc aagcgaggcc ctgatcgtga
     2161 tatagctgac cgtaagcagg gcaatggcga tggcgccggc gccggatagc atgaatacag
     2221 tccagtcgat gccggtgcgg taggcgtaac tgctgagcca gtggtgcatg agcacgccgg
     2281 cgacagggaa agcgatcagg caggcaaaga ggaccagcag gatgaagtcc cgggagagaa
     2341 ggctcgtcag cagcaggacg gaagcgccca gcactttccg gacgccgatc tcctttgtcc
     2401 ttcgctcggc tgcgtaccct gcaaggccga aaagaccaag gcaggagata aaaatggcga
     2461 gcgcggcgaa gacgcccgac agcctgccag tgagcgtttc ggtttggaac tggttttcaa
     2521 agtcctcatt caggaaatga tattcgaacg ggtatgcggg attggcggct ttcatgactg
     2581 ttccgatcgc ggcgacggcc ttggcgatgt cggcgcctgc cttcagcctt accgtcatga
     2641 cgttggtatt ggccgggtcg cagaaaagga tcagcggggc cgcgctttga tacatattgt
     2701 cgtacacaaa atccttgatg acaccgatca cccgccattt ttccttgccg ttttcgcggg
     2761 tgatcaggct gccgacaggg ttgcctttcg tgatgatatg ggcgagcgct tcgttgatga
     2821 tgatgttgtt gctgtcctgg gcagcgacgt catggaaatt gcgccccgcg gcgagcgtca
     2881 cgcccattgt agacaggtat tcgggcgatg cattctcgac cgtgatcagc acctgtttat
     2941 cagggtcctt gcccggccac tcgaaatcgc cggtattgct tcccaactgc aggatcgtgc
     3001 tgctgctgag tgtcgcgtct ttgacataac cggtatggag aagatcggtc cggacagcag
     3061 cgaaatggtg acccagatca ccttgcaggt tcatatagag aagcccttct ttttcaaaac
     3121 cgagatcccg gttgtggacg aaggtcagtt gccggtagat gatgatggtg gagatgatga
     3181 acacg
//
LOCUS       DATGKE010000306         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1536942, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000306 DATGKE010000000
VERSION     DATGKE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536942"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VKU83_05230"
     CDS             complement(<1..493)
                     /locus_tag="VKU83_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLI92985.1"
                     /translation="MTVLFRHIPFLKAVLLYSLTAFGGPQGHFAMMLKTFVQKRRDVT
                     AEELMEYNAFCQLLPGASSTQTLTLIGYKRGGVLLAVITMLIWTLPACILMGLFSFFP
                     TDNTAVFRYIPSMTIGFLAFAAQRAFRFSIRNTITRVILAITTIATFLLFKSPWIFPL
                     LLVL"
     gene            complement(505..1089)
                     /gene="nadD"
                     /locus_tag="VKU83_05235"
     CDS             complement(505..1089)
                     /gene="nadD"
                     /locus_tag="VKU83_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HLI92986.1"
                     /translation="MKVGLYFGSFNPIHHGHLLIANFVLQHAKLDQVWFVVSPQNPLK
                     RQAALLNEYHRLHLVRLAVEGEKHLRASDIEFKLPRPSFTVDTLAYLQEKYPEDEFSV
                     IMGSDSYVNLPKWKNYEWLLRHYSIYVYCRSDHEKLPEYPGAKNVQIMDAPVLPISST
                     DIRGKIKAGQSVRYLLPESVLQEIEKSGYYRNIR"
     gene            complement(1086..1316)
                     /locus_tag="VKU83_05240"
     CDS             complement(1086..1316)
                     /locus_tag="VKU83_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HLI92987.1"
                     /translation="MKRLSELEPGTVARIHSFEKNDLFLKLMEMGCVPGELVKVEQVA
                     PLGDPISITVAGYNLSLRLDEAENIFMEEGGK"
     gene            1356..1811
                     /gene="ybeY"
                     /locus_tag="VKU83_05245"
     CDS             1356..1811
                     /gene="ybeY"
                     /locus_tag="VKU83_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLI92988.1"
                     /translation="MTISPTIRFHFLKKVELRDRSRLKAAVVSLFRAERRKLGEIRYI
                     FCSDKYLLDINRRFLGHDYYTDIITFPLSEPSQPVVAEVYISVDRVRDNAARFHNPLK
                     TELLRVIFHGALHLCGYGDKTAREQVKMRALEDKYVLLFQRSMWNTRPK"
     gene            1893..>2590
                     /locus_tag="VKU83_05250"
     CDS             1893..>2590
                     /locus_tag="VKU83_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI92989.1"
                     /translation="MFPDYDVIVVGAGHAGCEAAAAAANLGSKVLLVTMNMQTIAQMS
                     CNPAMGGIAKGQIVREIDAMGGYSGIVSDRSMIQFRMLNRSKGPAMWSPRTQNDRMLF
                     AATWRQLLEETPNVDFYQDTVHSLIIRENTVCGVITGLGHRILAKAVVVTSGTFLNGV
                     IHIGEKKFGGGRVAEKAATGITEQLVSLGFESSRLKTGTPPRIDGRSIDYSKMEEQKG
                     DDEIVGFSFLPTEKP"
BASE COUNT          654 a          657 c          673 g          606 t
ORIGIN      
        1 cgagcaccag cagcagcgga aagatccacg gcgatttgaa caacaaaaag gtcgcgatgg
       61 tggtgatcgc caggataacc cgcgtgatgg tattgcgaat ggaaaatcga aaggcccgct
      121 gcgctgcaaa agccagaaaa ccaatcgtca tggaaggaat atagcgaaac acggcggtgt
      181 tgtcggtcgg aaaaaaagaa aacaaaccca tcaggatgca ggcgggtagc gtccagatca
      241 gcatcgtgat gacggcaagc agcacgccgc cgcgtttgta accaatcagc gtcagggttt
      301 gagtcgaaga ggcaccgggc aaaagctggc aaaatgcatt gtattccatc agctcttcgg
      361 ctgtcacgtc gcggcgtttt tggacaaaag tcttcagcat catcgcgaaa tgaccctggg
      421 ggccaccaaa ggccgtaaga ctgtagagca gaactgcttt aaggaagggg atatgacgga
      481 acaacactgt catccgtaca caaattatcg aatgttgcgg taatagccgc ttttttcgat
      541 ctcctggagc acggactcag gcagcagata acgtacagat tgcccggctt ttattttgcc
      601 ccggatgtcg gtcgatgaaa tcggaaggac cggcgcatcc ataatctgta catttttcgc
      661 acccgggtat tccggcagct tttcgtggtc cgaccggcaa taaacataaa tggaatagtg
      721 cctcaatagc cattcataat tcttccattt cgggagattg acataactgt cgctgcccat
      781 tatgaccgaa aactcgtctt ccgggtattt ttcctgtaaa taggcgaggg tatcgacggt
      841 gaaggaaggc cgcggaagtt tgaactcgat gtccgacgcc cgcaggtgct tttctccttc
      901 aaccgccagc ctcaccaggt gcaggcggtg gtattcattc aaaagggctg cctggcgttt
      961 cagcgggttt tgtggcgata ccacgaacca tacctggtca agcttcgcgt gctggagaac
     1021 aaaattggct ataagcagat gtccgtggtg tatagggttg aaggatccga agtataaccc
     1081 taccttcatt ttcctccctc ctccatgaaa atgttctccg cctcatccag ccggagacta
     1141 aggttatacc cggcgacggt aatggaaata ggatcgccga ggggcgcaac ctgctcaacc
     1201 ttaaccaact ctccgggcac gcagcccatt tccattagct tcaggaacag atcattcttc
     1261 tcgaacgaat ggattcgcgc aacggttccc ggttccagtt ctgacaatcg cttcatttct
     1321 tcaggtggtt tatcatacct ttcaacagta aaattatgac aatctcccct accatccggt
     1381 ttcatttttt gaagaaggtc gagctccgcg atcgtagtcg actaaaggcc gctgttgtca
     1441 gcctcttcag agccgagcgc cggaagctgg gtgagatccg ttatatcttt tgttcggaca
     1501 aataccttct cgatataaat cgccggttcc tcggtcatga ctattacacc gacattatca
     1561 ccttccccct ttcagaacct tcacaacccg ttgtggcaga agtatatata agcgttgaca
     1621 gggtcaggga taatgcggca agatttcata atcccctgaa aaccgagctt ttgcgcgtca
     1681 tttttcacgg tgccttgcat ctttgcgggt atggcgataa aactgcccgc gaacaggtca
     1741 aaatgcgtgc attggaagac aagtacgtcc ttttgtttca gcgttccatg tggaacaccc
     1801 ggccaaaata attgcgctga aatgttcccc gtggaacgtt tttaagcttc ttttattcta
     1861 ttatttgtcc acattcgggt taattttgca aaatgtttcc ggactatgat gtgatagtag
     1921 ttggggccgg ccatgccggt tgcgaagcag cagcggcggc agctaacctg ggttcgaaag
     1981 tgcttctcgt aaccatgaac atgcagacca ttgcgcaaat gagctgtaat cccgcgatgg
     2041 ggggaattgc caaaggtcag attgtccgcg aaatagacgc catgggcggt tactccggaa
     2101 tcgtcagcga ccgcagcatg atccagttca ggatgttgaa tcgctccaaa gggcccgcta
     2161 tgtggagtcc ccggacgcaa aacgaccgga tgcttttcgc ggcaacctgg cgccagcttc
     2221 ttgaagagac ccccaacgtg gatttctacc aggatacggt ccatagcctg attatccggg
     2281 aaaacacggt ctgcggggta ataaccggcc tgggtcaccg gatccttgcc aaggccgtag
     2341 tggtgacgag cggtaccttc ctgaacggcg tcatccatat tggcgagaag aagtttggcg
     2401 gcggtcgggt tgccgaaaaa gcggcaaccg gcataaccga acaactggtc agccttgggt
     2461 tcgaaagcag ccggctaaaa acgggtacgc caccacgcat cgatggccgt agcattgact
     2521 actcgaaaat ggaggagcaa aaaggcgacg acgaaatcgt tggtttttcc tttctcccga
     2581 cggaaaagcc
//
LOCUS       DATGKE010000307         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_274223, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000307 DATGKE010000000
VERSION     DATGKE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274223"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3184
                     /locus_tag="VKU83_05255"
     CDS             <1..3184
                     /locus_tag="VKU83_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HLI92990.1"
                     /translation="SDQGSCGDSPFKDGFLMPDRFETFDRFDVPGRSVRPIWVSINIP
                     ASAKPGHYTGTIEALGKGFTKLLKVNVEVQNQLLPPPHDWHFRLDLWQNPWVVAWQSH
                     IQPWSKEHLTLLQQHLKLYADAGGKYITTYAVNSPWNDNSYSIEGSMIESIREKDGNW
                     KFDYGIFDQYVTLAMSVGIDKAITIYTPVPDSYRFRYLDAATGDYETVYWAPGSDPWK
                     SYWGPFLNSLRDHLAEKGWLKKAYIGINENPLDETLTAIRFVKSNWPGWKITYAGDWH
                     PELKDLLDDYSYVKEKESPMDVVAARKARGQTTTFYICCQPAKPNTFIFSPPVEGRWI
                     GWYAWAYGYSGFLRWAYDAWTEDPNRDARHGSWPAGDCYMVYPGGNSCIRFEKMREGI
                     VDYEKMRILEQEVSASTDERCKELWRQLQGELALMTKEHVYRTDTLEKQVSDGVTLVR
                     ELSARLGDLQGDQPAAAAVSCSQYDDYKLTHELTPFGPVPTAFDPNGVYPYVSYAETS
                     HRPVLKKYHYIVLENSRIRVTICPDLGGKVTSMLIKGRGDSAGERSLKARTAAAIRGK
                     EVLYAPDVVRQERILPRFYFTAGGIEVSFPISHSPTENERVDYQVDEAPDRTYVTCGE
                     RELRFGLQWSVEYSLGSRDDFLTERVLYHNPGAEAYPWMSWSNAAIPASSDTRFHFPQ
                     GRVLSHSSRVDTIDWKTQGPKTQNDIKEMTGYFWETKDANAFGAYTPSSGTGLYHIAR
                     PDIAPGIKLWSYGVAADTAWSMLSTAKRQQYIELQGGPLTDQSVKTQLRPGETRSHTE
                     YWIPTDKELSIYQLTVPAPSLRPAGDIPVFGWARPGTVDIWDSLQRAFEEGSVLPAPP
                     AIDRCLWAPSGMEHLDAPFRWAIAGTAGSVSDQWKFNYGTWLAGRGRAEEAIKVLAES
                     ENGLAKVLLARLLKDKGDIAGARKALDDIRESWLQIDPQVIIERDKILRKIGPSTLGE
                     REKWLSKVGAMKDEWIIERKIQWLIDKGELVAAKKLLLSIPFQMVHQTYTRTGLWMQI
                     CDKQKVSPYPVPRQLGEDRLARFGAYREYE"
     gene            3291..3608
                     /locus_tag="VKU83_05260"
     CDS             3291..3608
                     /locus_tag="VKU83_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92991.1"
                     /translation="MMLPKRYVILAVFVASSALLFYLLFELLFHIFLSLSSGGPIDVG
                     AGIAAMACSGVGGVVLLILSSAIATVIYHRRGKKELEKGFLYSLWFSLLLVVAVLVGW
                     YML"
     gene            3638..>3967
                     /locus_tag="VKU83_05265"
     CDS             3638..>3967
                     /locus_tag="VKU83_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92992.1"
                     /translation="MSQPQRYIAHFDLDAFFVNVEILNDPSLKGLPLLVGGRERGVVA
                     ACSYEARKFGIHSAMPMKTAMRLCPQAVVVGSTRGEYSRYSRWVTEIIAARAPLFEKA
                     SIDEFYLD"
BASE COUNT          919 a          998 c         1180 g          870 t
ORIGIN      
        1 atcggatcaa ggaagctgcg gcgattcgcc gtttaaggat ggattcctca tgccggatcg
       61 ctttgagacc ttcgatcgct tcgatgtccc gggtaggtct gtcaggccga tttgggtaag
      121 catcaatatc ccggccagcg ccaagccggg ccattacacc gggacgattg aagccttggg
      181 aaagggtttt acgaaattgc tcaaggtgaa cgtcgaggtt caaaatcagc ttctgccccc
      241 gccgcacgat tggcatttca ggctggacct ctggcaaaac ccctgggttg tggcctggca
      301 aagccatatt cagccctggt caaaggagca tctgaccctc ttgcaacaac atctcaaact
      361 atatgcggat gccggtggga aatatattac cacctatgct gttaactcgc cctggaacga
      421 caactcgtac agcattgaag gcagcatgat cgaatccatt cgggaaaaag acggcaactg
      481 gaaatttgat tatggaattt ttgatcagta cgtcacgctg gccatgagcg tgggtatcga
      541 caaggcaatc acaatctata cgccggtccc ggactcatac cgctttcgat atctcgacgc
      601 tgcaaccgga gattatgaaa cggtatactg ggcgcctggc tccgatccct ggaaatccta
      661 ttgggggcca ttcctcaaca gcctgaggga ccatttggcg gaaaaaggct ggctgaaaaa
      721 agcctatatc ggcatcaatg aaaacccgct ggatgagacc ctcaccgcca tccgattcgt
      781 gaaatcaaat tggcctggct ggaagatcac gtatgccggc gactggcacc cggaattgaa
      841 ggaccttctg gatgattatt cgtacgtcaa agaaaaggaa tcaccgatgg atgttgtcgc
      901 ggcgaggaag gcgagaggtc agacgacgac gttttatatc tgttgccagc cggcgaagcc
      961 caataccttt attttttccc caccggtcga aggccgatgg atcgggtggt atgcatgggc
     1021 gtatggttac agcggcttcc tgcgttgggc ttatgacgcc tggacggagg atccgaaccg
     1081 cgacgcacgc catgggagct ggcccgcggg agattgctat atggtgtacc cgggcggcaa
     1141 tagttgcatc cgcttcgaga aaatgagaga aggcatcgtg gattatgaaa agatgcgaat
     1201 cctggagcag gaagtgtcgg catcaacgga tgagcgatgc aaggaattgt ggcgacaatt
     1261 gcagggcgag ctggctctca tgacaaagga acatgtgtat cgaacggata cgctggaaaa
     1321 gcaagtcagc gacggcgtaa cgctggttcg cgaactcagc gcccggctgg gggacctgca
     1381 gggagatcaa cctgccgccg cggcagtttc ctgctcgcaa tacgatgact acaaactgac
     1441 gcatgagctc acgcctttcg gcccggtccc cactgctttt gatccgaacg gcgtatatcc
     1501 atatgtcagc tacgcagaaa cttcccatcg gcccgttctc aaaaaatatc actatatcgt
     1561 tctcgagaat agccggatca gggtaaccat ctgcccggac cttgggggaa aggttacatc
     1621 aatgctgata aaggggcgag gagattccgc cggcgaacgc agcctgaaag cacggaccgc
     1681 cgcggcgata cgcggtaaag aggttttata tgcgccggat gtggtccggc aggagcggat
     1741 cttgccacgg ttctatttta cagcaggagg tatcgaagtg agcttcccga tctcacattc
     1801 accgaccgaa aacgaacggg tggattacca ggtggacgag gccccggaca ggacttatgt
     1861 gacctgcggc gaacgggagt tgcgcttcgg cttgcagtgg tccgtcgagt actcgcttgg
     1921 gtccagggat gatttcctga cggaacgggt tctctatcac aacccgggag cggaggcata
     1981 cccgtggatg tcctggtcaa acgctgccat tccggcgagc tccgatacga ggtttcattt
     2041 tcctcaaggt cgcgtactgt cgcattcctc ccgggtggac accattgact ggaaaacgca
     2101 ggggccaaaa acccaaaacg acatcaaaga gatgaccggc tatttttggg agaccaaaga
     2161 cgccaatgcg ttcggggcat acacgccgtc atcgggaaca ggtttatacc atatcgcgcg
     2221 gccggatatc gcgccgggaa ttaaactgtg gagctacggc gtggccgccg atacggcctg
     2281 gtcaatgctg agcacggcca agaggcagca gtatatcgag ctgcagggcg gccctttgac
     2341 cgatcaatcg gtcaaaactc agttgcggcc gggcgaaacg cggtcgcata cggagtactg
     2401 gataccgaca gacaaggaat taagcattta tcagctcact gtgcccgcgc cgtcgctcag
     2461 gccggccggg gacattccgg tttttggctg ggcgcgtcca ggtacggtcg atatttggga
     2521 tagcttgcag agggcattcg aagaaggatc tgtgctgccg gcgccacctg ccattgaccg
     2581 gtgcctttgg gcgccgtcag gaatggaaca tttggatgcg ccattccgat gggcaatagc
     2641 tggaacagcg ggttccgtat cggaccaatg gaagttcaat tatggcacct ggctggccgg
     2701 acgtggccgg gccgaagagg cgatcaaggt attggcagaa tctgaaaatg gtctcgccaa
     2761 ggtgcttctg gccagattgc tgaaggacaa gggcgatata gcaggcgctc ggaaggccct
     2821 agatgacata cgcgaatcct ggctgcagat cgatccgcag gtcatcatcg aacgggataa
     2881 gatattacga aagatcgggc cttcgacgct tggcgaacgg gagaaatggt tgtcgaaggt
     2941 tggcgccatg aaggacgaat ggattatcga gcgcaaaatc caatggctga tcgacaaagg
     3001 tgagctggta gcggcgaaaa agttgctttt atccatcccg tttcaaatgg tacaccagac
     3061 gtatacccgc accgggttat ggatgcagat ttgcgacaag cagaaggtat caccctaccc
     3121 ggttcccagg caattgggag aggacagatt ggcgagattt ggggcgtatc gggagtatga
     3181 ataagcttga aaatcagcgt cttttccgcc gaaaaccgga cggtttgtta accggaagtt
     3241 ttttagcggc cgccacgcgc gagattcgac ggattcgcta tatttaatat atgatgctgc
     3301 cgaaacgata tgttatcctg gccgtatttg tggcttcttc ggcgctattg ttttatcttc
     3361 ttttcgagct tttatttcac atctttttat ctctatcaag cggggggccg atcgatgttg
     3421 gcgcgggcat cgccgcgatg gcctgcagcg gtgtcggcgg ggtggtcctg cttattttgt
     3481 ccagcgcgat cgcgaccgtt atctatcacc ggcgcgggaa gaaggagttg gagaaagggt
     3541 ttttgtattc gctctggttt tctttgttac tcgtggtggc ggtgcttgtc gggtggtata
     3601 tgctctgacc cggaaactaa cggtaccttt gccacggatg tcccaacccc aacgttatat
     3661 cgcccatttt gacctcgatg ccttcttcgt gaatgtcgag atcctcaacg acccgtcatt
     3721 gaaggggctg cccctgctgg taggcggccg ggaacgcggg gtagtcgccg cgtgcagcta
     3781 tgaggcgcgc aaattcggga tccacagcgc catgccgatg aagacggcga tgcggctttg
     3841 cccgcaggcg gtagtggtgg gcagcacgcg gggagaatac agccgctatt cgaggtgggt
     3901 aaccgaaatc atcgctgcca gggcgccgct gtttgaaaag gcgtcgatcg acgagttcta
     3961 tctcgat
//
LOCUS       DATGKE010000308         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1365828, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000308 DATGKE010000000
VERSION     DATGKE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365828"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VKU83_05270"
     CDS             complement(<1..718)
                     /locus_tag="VKU83_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92993.1"
                     /translation="MKKFLRYSLLLLTPCAVKAQVDTAVMKALPDSTSRPRVAVFLPL
                     YLDSAFDAAGNYRYDKTVPKFIHAGLEYYEGMELAVDSLTKEGVRLDVQVYDTRSAGR
                     PPGAVLNSPGFQGTGLILADVANGGEEQQFAAAALRLHIPFINVNYPNDAGITGNPSL
                     VILNSTLRAHVEAIYRFLQRNYPTRPLIYFGKRGSQEDRLKGYFTELDHSTAGVPLKI
                     NYVTLDDNFDAGQLAVNMDSS"
     gene            complement(719..2257)
                     /gene="guaA"
                     /locus_tag="VKU83_05275"
     CDS             complement(719..2257)
                     /gene="guaA"
                     /locus_tag="VKU83_05275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HLI92994.1"
                     /translation="MTEKILILDFGSQYTQLIARAVREANVYCEITPFHKKVSFGPEL
                     KGIILSGSPFSVNDSDAPTVDVEALHHKVPVLGICYGAQLTAKWYGGSVEKSNKREYG
                     RALLHKQKEDTILNEVTELSQVWMSHADTILQLPAGFEVLANTETIPYAAFSKSRNNG
                     DLPLYCLQFHPEVYHSIEGKKILRNFLVNVCGCSQDWTPAHFVSDTVAALKKQIGGRK
                     VIMALSGGVDSTVAATLIHRAIGDRLVGIFVDNGVLRKHEFQQVLDTYRKIGLNVKGV
                     DASGLFYSELAGKSDPEAKRKTIGRLFIEVFQSEAAQVEGVGLLGQGTIYPDVIESMS
                     VHGPSMTIKSHHNVGGLPATMKLELVEPLRYLFKDEVRRVGAELGIPEEQLQRHPFPG
                     PGLAIRILGEVTPEKVRLLQEADHLYVQALKAHQLYSTVWQAGAILLPVKSVGVMGDE
                     RTYEFTVALRAVTSVDGMTADWAHLPYTFLADISNEIINNVKGINRVVYDISSKPPAT
                     IEWE"
     gene            2351..3082
                     /locus_tag="VKU83_05280"
     CDS             2351..3082
                     /locus_tag="VKU83_05280"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HLI92995.1"
                     /translation="MTRSTLTASLILLSFPLFLSSCNLLGRRVHGDGNITTQDRAVND
                     FKNIEVGGAAKVLVSQGDQHSVKVEVDENLQQYIEVFQVGNKVVIRQKDGYNVDPTGD
                     LKIYVTAPVYNDIDVSGACDIIGQAPITNPENLEMHISGAGEIKMEINAPQITAEITG
                     SGSVNLKGETKSVDIGLSGAADAHCFDLKAENTKVDISGAGSAEVYASVRLDASVGGA
                     GSVKYKGNAEVNEHITGAGSVHKVD"
     gene            3163..>3381
                     /locus_tag="VKU83_05285"
     CDS             3163..>3381
                     /locus_tag="VKU83_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI92996.1"
                     /translation="MVRQLQFLFLAMTLHGACRAQPGLPSAGLPSPELPSPERFINAV
                     FPTVVDSSLNHYFLLEGADTCRFVTYDYN"
BASE COUNT          726 a          984 c          920 g          751 t
ORIGIN      
        1 cggaactgtc catgttcaca gccagctgcc ctgcgtcgaa attgtcgtcg agcgttacgt
       61 agttgatctt taaggggacg cccgccgtcg aatggtcgag ttccgtaaaa tatcccttta
      121 accgatcctc ctgcgaaccg cgcttgccga aatagatcag cggccgcgtc ggataattgc
      181 gctggaggaa acggtagatg gcttcaacgt gcgcgcgtaa tgtcgagttg aggatgacga
      241 gcgatgggtt gcccgttatt cccgcgtcat tcgggtagtt gacattgatg aaggggatgt
      301 gcagccgcaa ggctgctgcc gcaaattgct gttcttctcc gccgttcgcg acgtcggcaa
      361 ggatcagtcc cgtgccctga aacccggggc tgttcaacac cgctccgggc ggccgccctg
      421 ccgaacgcgt atcgtagact tgcacgtcca gcctcacacc ttccttcgtc aggctgtcga
      481 cggccagttc catgccttcg tagtattcga gcccggcgtg aatgaatttg ggaacggtct
      541 tgtcatagcg gtagttgccc gcagcgtcga aggccgagtc caggtacaag ggcaggaata
      601 cggctacccg gggccgcgag gtgctgtccg gaagcgcctt catgactgcc gtatccacct
      661 gggcctttac tgcgcaaggg gtcaataaca acaaagaata tctgagaaat tttttcatct
      721 attcccattc gatcgtcgcc gggggcttgc tgctgatatc gtataccacc cggttgatcc
      781 ccttcacatt attgatgatc tcgttcgaaa tatccgccag gaaggtatag ggcaaatgcg
      841 cccagtccgc agtcattccg tcgacggacg tcacagcgcg caacgcaacg gtgaattcat
      901 acgtccgttc atcgcccatg acacccacgc tcttcaccgg cagcaggatg gcgcccgcct
      961 gccaaacggt cgaatacagt tgatgggcct tcagtgcctg gacatataaa tgatccgcct
     1021 cctgcaataa tcgcactttt tccggcgtga cttcgcccag gatccggatg gcgaggccgg
     1081 gtccggggaa cgggtgccgt tgaagctgct cttcagggat gccaagttcc gcgcccacgc
     1141 gccgcacctc atccttgaac agatatcgca gcggttcgac aagctcgagt ttcatcgtcg
     1201 ccggtagccc gccgacatta tggtgggatt tgatggtcat cgacggccca tggaccgaca
     1261 tgctttcgat cacatccggg tagatcgtgc cttgccccag gagtccgacg ccctccacct
     1321 gcgcggcttc cgattgaaag acttcgataa acaggcgccc gatcgtcttt cgctttgcct
     1381 ccggatccga tttgccggcc agctcgctgt agaacagccc cgatgcgtcg acgcccttaa
     1441 cattcaaccc gatcttgcga taggtatcca gcacttgttg gaattcatgc tttcgcaata
     1501 cgccattgtc gacgaaaatg ccgaccaggc ggtcgccgat cgcccggtgg atcagggttg
     1561 ccgcaaccgt tgaatccacc ccgccgctca atgccatgat gaccttcctt ccgccgatct
     1621 gctttttcag cgccgcaacg gtatcgctta caaaatgcgc gggggtccag tcctgggagc
     1681 aaccgcaaac atttaccagg aagtttcgca ggatcttctt gccttcaatg gaatgatata
     1741 cttccgggtg aaactgcagg cagtacagcg gcagatcgcc attgttccgc gacttggaaa
     1801 aggctgcgta aggaatcgtt tcggtattgg ccaggacctc gaagcccgcg ggcagttgaa
     1861 ggatcgtatc cgcatgactc atccagacct gcgaaagctc ggtgacctca ttcaggatgg
     1921 tatcctcttt ttgcttgtgc agcaaggccc ggccgtattc ccgcttgttg ctcttttcga
     1981 cgcttccgcc ataccatttc gcggtaagct gcgctccata gcagatgcca agcaccggca
     2041 ctttgtgatg caaggcttca acgtctaccg tcggagcatc cgaatcgttg accgagaacg
     2101 ggctgccgga aaggatgatc cctttcagct ccggaccgaa cgacactttc ttgtggaagg
     2161 gggtgatttc acagtacaca ttcgcctcac gtacagctct ggctatcaat tgagtatact
     2221 gagagccaaa gtcgaggatg agaatctttt ccgtcatggt gacgcgaagt taaaaaaaat
     2281 cccgcactgt aacctgccct gcttttactc gtcaaatctg ttacatttgt aagaacctta
     2341 aaaaatcccc atgaccagat caacactaac cgccagcctc attctcctca gttttcccct
     2401 cttcttgtca tcctgcaatc tcttgggcag gcgggtacac ggcgacggga atatcaccac
     2461 ccaggaccgg gcggtaaatg attttaaaaa cattgaggtc ggcggtgcgg caaaagtgct
     2521 ggtgtcgcag ggcgaccagc attcggtgaa agtcgaagtg gacgagaacc ttcaacaata
     2581 cattgaagtc tttcaggtcg gcaacaaggt agtcatccgg cagaaggatg gctataacgt
     2641 cgatcccacc ggagatctca agatatatgt tactgctccg gtctacaatg acatcgatgt
     2701 ctccggcgcc tgcgatatca tcggacaagc gccaatcacc aaccccgaga acctggagat
     2761 gcacatcagc ggcgccggcg agatcaaaat ggagattaat gcgccacaga tcaccgcgga
     2821 aatcaccggc agcggcagtg tgaacctgaa gggcgagacg aagagtgtgg atattggtct
     2881 gagcggggct gccgacgccc actgtttcga tctgaaagcg gagaatacga aggtcgatat
     2941 ttccggcgcc ggttcggcgg aagtctatgc cagcgtccga cttgacgcga gcgtcggcgg
     3001 agccggctcg gtgaaatata aaggcaatgc cgaggtcaac gaacacatca ccggggccgg
     3061 cagcgtccat aaggtggact gaagcggttg ctgtgggtga ggtgcgccgc ggcagccggc
     3121 gttttagcgc ggtcttaact taatatttcg gaatttggcg ccatggtgcg ccaactgcag
     3181 ttccttttcc tggccatgac cctccatggc gcctgccgcg cccagcccgg gctcccctcg
     3241 gcggggctcc cctcgccgga gcttccctcg ccggagcggt tcatcaacgc cgtcttcccc
     3301 acggttgtgg atagctcgct caaccactat ttcctgctcg aaggcgccga cacctgccgc
     3361 ttcgtgacat acgattataa t
//
LOCUS       DATGKE010000309         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1269222, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000309 DATGKE010000000
VERSION     DATGKE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269222"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VKU83_05290"
     CDS             complement(<1..1065)
                     /locus_tag="VKU83_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI92997.1"
                     /translation="MLRNWLLTALRGFRRNKLSTAINLLGLTVGLSSCLVIALFIRHE
                     LSYDNFEVNGPRIVRVIMQYRFGGGGDMQAGNFTSTHVAPRLQQVFPEVEAGVRMVSD
                     DQIVKYREKLFTEQRFMFADSSFFRVFSMPLLEGDPRIALSGPHKLVMTRSTARRYFG
                     DADPVGKIVRIGTDSSDYEVTGLIADCPSNSQIKFHLLASFSSLYDDDNPTYWDANYT
                     TFLLLRNKNDIAPLQAKVTAFMKKEMQGKGAEINFLLEPFMRIHLYSPYSGFEPGTSI
                     IYIYILAGVALLILVIAGATYVNLSTAHSVDRAREVGVRKVIGAGKSQLFWQFIGESV
                     LLCLVAVVLSLCVVLLVLPAF"
     gene            1282..1476
                     /locus_tag="VKU83_05295"
     CDS             1282..1476
                     /locus_tag="VKU83_05295"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HLI92998.1"
                     /translation="MDENVNPRRSGRVWAGLFLVGIGAVLVLDQMGFPLPDWLFNWPA
                     ILIAIGLLSGSGMDFGAVPG"
     gene            1578..2243
                     /locus_tag="VKU83_05300"
     CDS             1578..2243
                     /locus_tag="VKU83_05300"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HLI92999.1"
                     /translation="MFVLNPGRGRHEIYWNDDDWRNRNRASKDQWRQQRHEWKHQWKQ
                     DWKRKWRQHRYGWRSGSDIPTGSGGAASGSTEPADPVNPGGGGASGSAGAGGTSDFAS
                     GSSAGYTSEDFVDTTTFFGGIHKRIVSKNFKGGDVVTIMGGTELNLTQADFTGTVKLE
                     VVQIFGATKIIVPASWEIRSEVTAIFAGFEDKRQQPTPHNPDKILILHGTSLFGGIEL
                     RNY"
     gene            2271..2630
                     /locus_tag="VKU83_05305"
     CDS             2271..2630
                     /locus_tag="VKU83_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HLI93000.1"
                     /translation="MNWYLSKIIFQIICGEGRHTPQFDEQLRLIAAHDEKEAFTKAVQ
                     LGEKEQDRFYNHDRQLVQWKFINVCELYRLHNMLDGAEMYSRIQETDDPEGYIELANR
                     RAHHIRQFGTHKMLQLL"
BASE COUNT          653 a          773 c          723 g          578 t
ORIGIN      
        1 gaacgccggc aataccagga gtaccacgca tagactcagc acaacggcaa ccaaacacag
       61 cagcaccgac tcgccgatga actgccagaa cagttggctc ttgccggcgc ctataacctt
      121 gcggacgccg acttcgcgcg cccggtcaac cgaatgcgcc gtgctcagat tgacataggt
      181 cgcacccgcg atcaccagga tcagcagggc cacgcccgca agaatataaa tatatataat
      241 gctcgtgccg ggctcaaaac ccgaataagg tgaatagaga tgtatccgca tgaatggctc
      301 gagcaggaaa ttgatctccg cccccttgcc ctgcatctcc ttcttcatga aggccgtcac
      361 cttggcctgc agcggcgcga tatcattttt atttctcaat agcaggaagg tcgtataatt
      421 cgcgtcccag tacgtcgggt tgtcatcgtc atagagcgag gaaaacgagg ccagcagatg
      481 gaacttgatc tgggaattgg acgggcagtc ggcaatgagg ccggtgacct cgtaatcgct
      541 gctgtccgtg cctatacgga cgattttgcc gacgggatcg gcatcgccga aatagcgtcg
      601 cgccgtcgac cgggtcatga ccagcttgtg tgggcccgat agcgcgatgc gcggatcgcc
      661 ttcgagaagt ggcatgctga agacccggaa aaagctggaa tccgcaaaca tgaatcgttg
      721 ctccgtaaag agcttctccc tgtatttcac tatctgatcg tcgctgacca tcctcacccc
      781 cgcttcaact tcagggaata cctgttgcag cctcggagca acatgcgtcg aagtaaaatt
      841 tccggcctgc atatcacctc cgccgccaaa ccggtattgc atgatgaccc ggacgatccg
      901 cggcccgttg acctcgaaat tgtcgtagct cagttcgtgc cggataaaga gggcgatcac
      961 gagacaactc gacagcccga ccgttagccc gagcaaattg atagcggtgg acagtttgtt
     1021 tctgcgaaaa ccgcggagcg cggtcagcag ccagttacga agcatattac gatattaaac
     1081 aattatttaa tgtaaacttc cttacattta cccccacatt ctaagagacg cttgccaccc
     1141 gccctttgtt acatgccgtt cagctatgaa aatgctttac cgacaaaata ccccccttta
     1201 ccgtcccccg ggcgcccgct ggcgatcccc ccttgaccgg acccgccgaa caaactagat
     1261 ttgagaaaaa attgtcattc aatggacgaa aatgtaaatc caagacgatc gggccgggtc
     1321 tgggcaggat tattcctggt ggggattggg gccgtgctcg tgctggatca gatgggcttt
     1381 cctctacccg actggctctt taactggccc gccattctca tcgcaatcgg gctcttatcg
     1441 ggatccggca tggatttcgg ggcggtccct ggctgatcct catccttatc ggcacggcct
     1501 tcgagttcaa caattattat cctcacgccg agctccaccg gtatattttt cctggcatcc
     1561 tcatcctgat agggatcatg ttcgtcctca accccgggcg ggggcggcat gaaatatatt
     1621 ggaacgacga cgactggcgg aacaggaaca gggcgtcgaa ggaccaatgg aggcaacagc
     1681 ggcatgaatg gaagcaccaa tggaagcagg attggaaacg gaagtggcgg caacaccgct
     1741 atggctggcg aagcggttcc gatatcccaa cggggtcagg cggcgcggcg tcgggaagta
     1801 ctgaacccgc agacccggtc aatccgggcg gcggaggggc atcaggctcg gccggcgccg
     1861 gcgggacatc agactttgca agcggcagct ctgccggcta cacctccgaa gattttgtcg
     1921 ataccacgac gttttttggg ggaatccaca aaaggatcgt ttcgaagaat ttcaagggcg
     1981 gcgacgtggt tacgatcatg ggcgggacgg agctcaatct tacccaggcc gatttcaccg
     2041 ggaccgtgaa gctcgaagtc gtacagattt ttggcgctac caagattatc gttcccgcaa
     2101 gctgggagat ccgcagcgaa gtaacggcaa ttttcgccgg cttcgaggac aagcggcagc
     2161 aacccacgcc ccacaatccc gacaaaatcc tcatccttca tggaacctcg ctttttggcg
     2221 gcatcgaatt acgtaattat tgacaggcat taccatctaa ctttttccgt atgaactggt
     2281 atctttccaa gatcatcttt caaatcatct gcggtgaagg caggcacacg ccgcagtttg
     2341 acgaacagct ccgcctgatc gcagcacacg acgaaaagga ggcgtttacg aaagccgttc
     2401 agcttgggga aaaggaacag gaccggttct acaaccatga ccgacagctg gtccagtgga
     2461 aattcatcaa tgtctgcgag ctgtatcgtc tccacaatat gctcgatggc gcggagatgt
     2521 attcccgcat ccaggagacg gacgatcccg aaggatacat cgagctggca aaccggcgcg
     2581 cccaccatat ccgccaattc ggcacccaca aaatgttgca gttgctgtag tatggcaaaa
     2641 tctcccttct cttcgggcaa acttctgacg attttcgttt cctggtgggc catctgggcc
     2701 gtgctgcaaa ccagcgtcct gatcggt
//
LOCUS       DATGKE010000310         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_470986, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000310 DATGKE010000000
VERSION     DATGKE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470986"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2123)
                     /locus_tag="VKU83_05310"
     CDS             complement(207..2123)
                     /locus_tag="VKU83_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLI93001.1"
                     /translation="MRCKPYFFCLLLLPVCLQAQRNPAEGDFVKANYDKQEVYIPMRD
                     GIRLYTVIYTPKDKTHPHPFLMERTPYGSGPYGDSVYRRSLGPNRDLMHELYIVVYQD
                     VRGRYMSEGQFREMTPARNAPHNAERKGAAAKQVDESSDTWDTVDWLLRNIKNNNGRV
                     GIWGISYPGFYASASLPDAHPAIKAVSPQAPVTDEFIGDDANHNGAFFLLDNFDFDNF
                     FDVKRPEPVKNYSGDIFRVHYADAYQFFLHLGPLKNSNNAEYFNNQGKIWNEYLAGST
                     YDDYWQSRNIRPHLNNVRPATLVVGGWFDAEDMFGSLRTYEAIEKQTPGNDNHIIMGP
                     WTHGGWAAGSWTKFGILDFGQNVNDYYHKVETAFFNHYLLPNDSNALPEAMIFETGTN
                     QWKTYSTWPPAEAKATKFLLEPHFAIESPGSPLSPVAESQANMDAAPINTSPSPDWDE
                     YVSDPAHPVPYVDGIHERRDNQYIVTDQRFAAERPDVLSYQTSPLSGDLTVTGRLRPE
                     IWLSSTGSDADLIVKLIDVYPDTSGNPGYQRLVRAEVFRCKFRNSYEKPEPLVPGQPA
                     EIAFDMNEIAHCFKKGHRIMVQLQSSWFPLVDLNPQKFVNIPTADAADFQKATIRIWH
                     DAAHPSGITLPVMP"
     gene            complement(2130..4025)
                     /locus_tag="VKU83_05315"
     CDS             complement(2130..4025)
                     /locus_tag="VKU83_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLI93002.1"
                     /translation="MRKLLFFLLFSVSIAFAFGQGTAQDSAWIRENYVKVETYIPMRD
                     GIRLFTTIYRPKDTLDKHPILMTRTPYSCAPYGPERWRNYYSSYLRYYLHAGYIMVTQ
                     DVRGRWMSEGQFEDIRPFIKDKATKTDVDEASDTYDAIDWLIKNVPGNNGRVGVFGIS
                     YPGFYSTMASASAHPALKAVSPQAPVTDWFMGDDFHHNGAFYVMDGWAFYSSFGKPRP
                     QPTTVSPTGFEYYSHDNYKWYLETGALKNILNLTGDTIAFWKELYQHPNLDDWWKARN
                     ARVALYNIKPAMLVVGGLYDAEDCFGAWHVYKALKDQSPATNSHIVMGPWYHGQWASN
                     DGTHLGNVQWGSNTSWWYQNNIEIPFFNYYLKGEGIEPKLPAATIFFTGANEWRKFDQ
                     WPPAQSQDQAIYLQPGGGLAWSAPTKKSDFSEYISDPSHPVPYTEKVHFFRTREYMDD
                     DQRFASRRPDVLTFQTPVLSEDLTLAGPVIADLLVSLTSTDADFIVKVIDVFPDDFSY
                     AKQGPAPVRSHDNSEAYPMGGYQMLVHGEVMRGRFRESFEKPVPFKPGKVEEVKWAIP
                     DVAHVFKKGHRLMIQIQSSWFPLVDRNPQKFVDIYTADDSDFTKETIRVWHDADHASK
                     IILPVLK"
     gene            4095..4559
                     /locus_tag="VKU83_05320"
     CDS             4095..4559
                     /locus_tag="VKU83_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLI93003.1"
                     /translation="MNEEERLIDRFYANLRARDWERMVQLYHPGIFFYDPVFGNLDGA
                     QVTAMWRMLLSGVNQLDLKLSDLTGGDGYASCRWTAIYLFTPTGRKVVNHGRAMFRIG
                     EERILEHHDEFSFWRWSAQALGWKGRLFGWTTVFQKTVRRRALRQLERFMKK"
     gene            4566..>4976
                     /locus_tag="VKU83_05325"
     CDS             4566..>4976
                     /locus_tag="VKU83_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HLI93004.1"
                     /translation="MVNRRWFLQSSLLGSFGLLTGRRAVAAPARAAAVTGHPVVISTW
                     DTGIAANKGAWEVLSRNGSALDAVEAGVKVTESTINCCVGLGANPDRDGFVTLDASIM
                     DHQFNCGSVGALERIKHPISVARRVMEKTPHVILV"
BASE COUNT         1033 a         1211 c         1580 g         1152 t
ORIGIN      
        1 cttgttgcca ccgggtgctg acaggttcgg ggatgatgga tgcggggggc tggtcggtgg
       61 atgttgcggc ggccttgtca gtggattgtg cggcggagct aaggccggcg aatagcaggg
      121 ccagcgccgg cagaagaaat aggtgtttgg tcataaggcg aatttaggcg aaaatgggag
      181 gccggcgaac ggcctcccgc aaaaagttag ggcatgacag gcagtgtgat tccggaggga
      241 tgggccgcgt catgccagat tcggatggtg gctttttgaa aatcggcggc atccgcagtg
      301 gggatattga cgaatttctg cgggttgagg tcgacaagcg ggaaccagct gctctgaagc
      361 tggaccatga tgcgatggcc tttcttaaag cagtgcgcga tctcgttcat atcgaaagca
      421 atctccgcgg gttggccagg gacgaggggt tcaggtttct cgtagctgtt acggaacttg
      481 cagcgaaaga cctccgccct gaccagtcgt tgatagccgg gattgccgct ggtatcagga
      541 taaacatcga tgagctttac gatcaggtcc gcatccgagc cagtgctgga aagccagatt
      601 tccggccgca gccgtccggt aaccgtgaga tcgcccgaaa gggggctggt ttgataggac
      661 aacacgtcgg gccgttcggc ggcaaaacgc tggtcggtca cgatatactg gttgtcgcgc
      721 cgttcgtgga tgccgtcgac atacgggacg ggatgagcgg ggtcgctgac gtattcatcc
      781 cagtcgggtg agggggaggt gttgatcggc gcggcgtcca tgttggcctg cgattcggca
      841 acgggggaca ggggcgatcc gggagattcg atggcaaaat gcggctccaa aaggaatttc
      901 gtcgctttgg cttcggcggg cggccaggtg gaataggtct tccattggtt ggtgcctgtc
      961 tcaaagatca tggcttcggg gagggcgttg ctgtcattgg gcaacaggta atgattgaag
     1021 aacgccgtct cgaccttgtg atagtagtcg ttgacgttct gaccgaaatc aagaatcccg
     1081 aattttgtcc agctgccggc tgcccagcct ccgtgggtcc aggggcccat gatgatgtgg
     1141 ttgtcgttgc ctggtgtctg cttctcgatc gcctcatagg tgcggaggga cccgaacata
     1201 tcctcggcat cgaaccagcc gccgacgaca agcgtggcgg ggcggacatt gtttagatgc
     1261 ggccggatat tgcgggactg ccaatagtca tcgtacgtgg agcccgcgag gtactcgttc
     1321 cagattttac cctggttgtt gaaatattcc gcgttgttgc tgttcttcag cggacccagg
     1381 tgcagaaaga actgataggc gtcggcgtaa tggacgcgga agatgtcccc actgtaattt
     1441 ttgacaggct ccggcctttt gacatcgaag aaattgtcaa aatcaaaatt gtccagcagg
     1501 aagaaggcgc cgttgtggtt ggcgtcgtcg ccgatgaact cgtcggtcac cggcgcttgc
     1561 ggcgaaacgg ccttgatagc tgggtgtgcg tcgggaagcg aagcggatgc atagaaaccc
     1621 gggtaggaga tgccccagat cccgacgcgg ccgttgttgt tctttatgtt cctgagcagc
     1681 cagtcgaccg tgtcccaggt atcggaactt tcatcgacct gctttgcggc agcgcccttc
     1741 ctctcggcgt tatggggggc atttcgggcg ggtgtcatct cccggaattg cccttcactc
     1801 atatatcggc cgcgcacgtc ctggtacacg acaatataca gttcgtgcat aaggtcgcgg
     1861 ttcggtccga gggaccggcg atataccgag tcaccgtatg ggcctgaacc gtagggcgta
     1921 cgctccatca ggaagggatg gggatgggtc ttgtcctttg gcgtgtagat gacggtatag
     1981 aggcggatgc cgtcccgcat tggaatatag acctcctgct tatcgtagtt agccttcaca
     2041 aaatcgcctt cggccgggtt gcgttgggcc tgcaggcaca cgggcagcag taaaaggcaa
     2101 aagaaataag gcttgcagcg catatgcggt tattttagga cgggaagaat aatcttcgaa
     2161 gcatggtcgg cgtcgtgcca gacgcggatc gtctccttgg tgaaatcact gtcgtccgcc
     2221 gtgtagatgt ccacgaactt ctgcggattc cggtcgacga ggggaaacca gctgctttgg
     2281 atctgaatca tcagccggtg gcctttttta aagacgtggg cgacgtcggg gatcgcccat
     2341 ttcacttctt cgacctttcc aggcttgaag ggaacgggct tttcaaagct ttcccggaaa
     2401 cggccacgca tgacctcgcc atgaacgagc atttgataac cgcccatcgg gtaggcctca
     2461 ctgttatcat ggctccgcac cggagcgggg ccctgtttcg cgtagctgaa gtcgtccggg
     2521 aaaacatcga tgacctttac aataaagtcg gcgtccgtac tggtcaggct gacgagcaga
     2581 tcggcgatga ccggtccggc gagcgtcaga tcttcgctca aaacgggcgt ttggaaagtc
     2641 agcacgtcgg gccggcggga agcgaagcgc tggtcgtcgt ccatgtactc gcgtgtgcgg
     2701 aaaaaatgca ccttttcggt gtaggggacg ggatgggacg gatcgctgat gtattcactg
     2761 aaatcgctct tttttgtcgg ggcgctccag gcgaggccgc ctcccggctg caggtaaatg
     2821 gcctgatcct gcgactgagc gggtggccac tggtcaaatt tgcgccattc gtttgcgccg
     2881 gtgaagaaga tcgtggcggc gggcagcttc ggttcgatgc cttcaccttt aaggtagtaa
     2941 ttgaaaaagg gaatctcgat attgttttga taccaccacg acgtattgga tccccattgg
     3001 acattcccca ggtgggtgcc atcgttcgac gcccattgtc catggtacca gggtcccatg
     3061 acgatatggc tattcgtagc ggggctttgg tccttcagcg ctttatatac atgccatgcg
     3121 ccgaaacaat cttcggcgtc ataaaggccg ccgacgacga gcatggcagg cttgatattg
     3181 taaagggcaa cgcgggcatt gcgggccttc caccagtcgt cgaggttggg atgctggtag
     3241 agctccttcc aaaatgcaat cgtatcgccg gtaagattga ggatgttttt caatgcacct
     3301 gtttcgagat accacttgta gttatcatgg ctgtaatatt caaagccagt aggcgagacg
     3361 gtcgtaggtt gcggacgcgg cttgccgaat gaggagtaaa acgcccagcc gtccatcaca
     3421 taaaaggcgc cgttgtgatg gaagtcgtcg cccatgaacc agtcggttac cggggcttgg
     3481 ggactgacgg ccttcaacgc cggatgggcg ctggccgagg ccattgtcga ataaaaaccc
     3541 ggataggaaa tgccgaatac gccgacccgg ccgttgttgc cggggacatt ttttatcagc
     3601 cagtcgatcg cgtcataggt gtcgcttgct tcatcgacgt cggtcttggt ggccttgtcc
     3661 tttataaacg gccggatgtc ttcgaactgc ccttcgctca tccagcggcc ccggacgtcc
     3721 tgtgtgacca taatgtagcc ggcgtgaagg tagtagcgaa ggtagctgct gtagtagttg
     3781 cgccagcgct cgggaccgta aggcgcacaa gagtagggcg tgcgtgtcat caggatcggg
     3841 tgcttgtcca aggtgtcttt cgggcgatag atcgtagtga agaggcggat gccatcgcgc
     3901 atcggaatgt atgtctcgac ctttacgtaa ttctcccgga tccaggcgct gtcctgtgcg
     3961 gtgccctggc caaatgcgaa cgcaatacta acggaaaaaa gcagaaaaaa aagaagcttt
     4021 ctcatggtga aacagttaat tttagacgcc attcgaattt agggtaaacg cggctaatcg
     4081 agcaggtttt ttttatgaat gaagaagagc ggttaataga tcgtttttat gcgaatttgc
     4141 gtgcgcgtga ctgggagcgg atggtgcagc tgtatcatcc cgggattttt ttctatgacc
     4201 ccgtctttgg aaaccttgac ggggcgcagg ttacagcgat gtggcggatg ctcctcagcg
     4261 gtgttaacca actggatctg aagctcagcg atttgaccgg cggggacggc tacgcgtcct
     4321 gtcgctggac agcgatatat ttatttacgc cgaccggccg gaaagtggtg aaccatggcc
     4381 gcgcgatgtt tcgcatcggg gaggaaagga tccttgagca ccacgacgaa ttttcgtttt
     4441 ggcgatggag cgcgcaggcg ttgggctgga agggaaggct atttggttgg acgactgttt
     4501 tccaaaagac agtacggcgc cgggcgctgc ggcaactgga acgatttatg aaaaaatgat
     4561 atattatggt aaacaggcga tggtttttgc agtcttcctt gttaggttca ttcgggttgc
     4621 tcaccggcag acgtgccgta gcagctcccg cgagggccgc cgcggttacc gggcatcccg
     4681 tcgtcatctc gacatgggat acgggtattg cagcgaataa gggcgcctgg gaggtgctga
     4741 gccggaacgg gtctgccctg gatgctgtgg aggcaggggt aaaggtcacc gagtcgacga
     4801 tcaattgttg cgtcggctta ggcgcgaatc cggaccggga tggtttcgtg accctcgacg
     4861 catcgatcat ggatcatcaa ttcaattgtg gaagtgtggg cgcactggag cgaatcaagc
     4921 atcctatttc tgttgcgcgg cgcgttatgg agaaaacgcc gcacgtgatc ctggta
//
LOCUS       DATGKE010000311         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_434048, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000311 DATGKE010000000
VERSION     DATGKE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434048"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..426)
                     /locus_tag="VKU83_05330"
     CDS             complement(274..426)
                     /locus_tag="VKU83_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93005.1"
                     /translation="MNKRRDIAVVVAIVVGLILGRIIERVTLGLLIGLGIGLLVASLI
                     SNRRRR"
     gene            complement(433..2961)
                     /locus_tag="VKU83_05335"
     CDS             complement(433..2961)
                     /locus_tag="VKU83_05335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLI93006.1"
                     /translation="MNKVIGFFLAGLAFAAGVRGQGPATVTSADIYLRIKKLNVLGSV
                     LYIAAHPDDENSRLIAYMADGRLYRTAYLSMTRGDGGQNLIGDEQGVELGLIRTQEMM
                     AARRVDGAEQFFTRAYDFGFSKSTEEALKTWDQDKILSDVVWVIRKYQPDVIITRFPP
                     DSRAGHGHHSASALLAHEAFDAAADPNRFPEQFKYGVKPWRARRVLWNSFNFGRNFNT
                     TSEDQMKIDVGGFNPIVGKSYGELAAISRTNHKSQGAALTPSRGQSMEYFTLVAGDPA
                     PDDPMDGVETTWNRVPGAEGISALVDGVLKEYSLTAPEKSVPGLVEVYKMIEKLPAGY
                     WRTEKLKEVRQLIEDCSGLWLEATAQSPYIAQGDSLPVTIVLNNRLGADLALKEVRLG
                     TPGEWGEAPLAGRPDLDTVWNERLDNDRNYSFTRYLRVATTKSVTQPYWLEQPMSLGH
                     FNVSDQRLIGDAQSAPAYVAEFTLMVDGQEMVLTRPLQYKTTDPVRGELYEPLTVEPR
                     YTARLDPEMLVFPDKAAKDFEADLVRHSAAARDVRQSFTPVTGLALSPGAGLDGPGSK
                     EWAARPNGQATGLVSGDLLIDHEGAGQRNDTALQLLTISYQHIPRIDYFRPAEEKFVV
                     ADIKTAGHRIGYIEGAGDKVPQALQQMGYDVVVLKEGDLASAAYLQQYDAIIAGVRAY
                     DVHPWLTGRHDVLMEYVKNGGNYIVQYNRGGLGQMKTTIGPYPFAVANIRVTDENAEV
                     NFVLPEHKVLHYPNKITEKDFSGWIQERGIYFAGETDTAYKEVLSMKDPGEPEQKGSL
                     VIAGYGKGDFVYTGLVFFRELPAGVPGAYRLMANIIALNQKKAF"
     gene            complement(3028..>4847)
                     /locus_tag="VKU83_05340"
     CDS             complement(3028..>4847)
                     /locus_tag="VKU83_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HLI93007.1"
                     /translation="GWNSPYYFAPAAEPYHDVITPWQRQFQVLIGKNNAKYFDRSGWL
                     FYTRQEYDLFYPSYGDTYPTYNGAVGMTYEQGGIGAGLAVRTNDGDTLRLADRLEHHF
                     ITGMSTIETASLHAGQLLKAFHQYFVDAVNRPGGEFKAYFIRSDSFGDRLRRLKGLLD
                     RNRILWSEAPAATYLGLNYETGRMSQFRSVPGDIVINANQPKSNLLRVLLERQSRFSD
                     SITYDITAWSIPFVYGLQAYGLGSYVSGAGAAASDSAVYRDAEEGDYAYAVRWSGLSS
                     AKFLSQVLQKGIKVRYAERPFQAGGASYDRGTLLITSAGNGAAGPKWRQTVVECARTT
                     GVNMSTMTSGFVEKGPDFGSDLVRMVHAPRVAMLSGDQVSSQEAGEVWHLFEQELHYP
                     VTLINASDVRQASWKDFDVLILPNGNYRLLEDKTVEEGLRSWVHEGGRLIAMQDAVGQ
                     LAKGDWGIKEKGEDAEKQATEDSGRVDEDALLRRYGDREREEAETSVRGAIYRIELDD
                     THPLAFGYPDHYYTLKQDDKVYEFMREGWNVGVIRKDGYISGFTGNKAKERLKEGLIF
                     GVRNLGRGNVICMADDPLFRSFWENGKLLFCNAVFLVGQ"
BASE COUNT          964 a         1607 c         1211 g         1065 t
ORIGIN      
        1 cggcttgtcc tgctcttata cagcccatag gcaatgatgc cgagcagggt caagacgagt
       61 acgatccagt ccggacggct catgaaaaat gtttggtgag ataatagaag actccgatac
      121 acagcaacag gaatccggcc accaggatat accagtagct ccaccttgaa aagagcggaa
      181 tccgctcgtt agtatcctgt ttttcttcca tacttgtaac gggaaaccgg cccttctccg
      241 ccgccgcccg cggcattcgt cccaagatcc gcgctatcgc ctcctcctgt tggagatcag
      301 cgacgccacc agcaatccga tccccaaccc gatcagcaac cccagcgtta ctctttcaat
      361 tatccggccc agtatcaggc caaccacaat agccacgact acggcaatgt ccctcctttt
      421 attcatatct tcttaaaacg ccttcttttg gttcaaagcg ataatattcg ccatcaaccg
      481 gtaagccccc ggcacgccgg caggcaattc ccggaagaat accagtcccg tatagacgaa
      541 atctcccttt ccgtaaccgg caatcaccag actgcctttt tgctcgggtt ccccggggtc
      601 cttcatggac aatacttctt tataagccgt gtcggtctct ccggcaaaat aaatacccct
      661 ctcctggatc cagcccgaaa aatccttttc cgtgatcttg ttgggatagt ggagcacctt
      721 gtgctccgga agcacaaaat ttacttccgc attttcgtcc gttacccgga tattggctac
      781 cgcgaacgga tacgggccga tcgtcgtttt catctgtcca agcccgcccc tgttgtactg
      841 cacgatgtag ttaccgccgt tcttcacata ttccatcagg acatcgtgcc ggccggtcag
      901 ccaggggtga acatcatagg cccggactcc ggcaatgatc gcgtcgtatt gctgcaggta
      961 tgccgccgat gccagatcac cttccttcaa caccactacg tcatagccca tctgctgcaa
     1021 agcctgcgga accttatctc ctgcgccctc gatgtaaccg atccgatggc ctgccgtctt
     1081 gatatccgcc acgacaaatt tttcttccgc cggccggaag taatcgatcc tggggatatg
     1141 ctggtaagaa attgtcagca gttgcagcgc cgtgtcattc ctttgcccgg ccccctcatg
     1201 gtcgatcagc aggtcgcctg acacgagacc cgtcgcctgg ccgttgggcc gggccgccca
     1261 ttcttttgat ccggggccat ccaatccggc gcccggagaa agcgccaggc cggtcaccgg
     1321 cgtgaaggat tgccggacgt cccgcgcagc ggcactgtgc cggaccaggt ccgcctcgaa
     1381 gtccttggcg gccttatccg ggaatacgag catctccgga tccagcctgg ccgtataccg
     1441 gggctccacg gtcagcggct catatagttc tccgcggacc gggtcggttg tcttatactg
     1501 gaggggacgc gtcagcacca tctcctggcc atccaccatc agggtaaact ccgcgacata
     1561 cgccggtgcg ctctgtgcat ccccgatgag ccgctgatcc gaaacattga aatgacccag
     1621 cgacatcggt tgctccaacc agtacggttg cgtgaccgac ttggtggtcg ccacccgcag
     1681 gtaccgcgtg aaagaatagt tccggtcgtt gtcaagccgc tcgttccaga ccgtatccag
     1741 atccggccgc ccggccagcg gcgcctcccc ccattcgccg ggagtgccta accttacctc
     1801 tttcaacgcc agatcggcgc ccaggcggtt gttaagcacg atcgtcacgg gcagcgaatc
     1861 gccctgggca atatacggcg attgcgccgt ggcctcgagc catagcccgc tgcaatcttc
     1921 gatcaactgg cgcacttcct tcaatttctc cgtccgccaa taacccgcgg gcaatttttc
     1981 aatcatctta tatacctcca ccagcccggg cacgcttttc tcgggcgctg ttagcgaata
     2041 ttctttaagt accccgtcga cgagtgcgct gatcccttcc gcgcccggca cgcggttcca
     2101 ggtcgtttcc accccgtcca tcgggtcgtc aggcgccgga tcgcccgcca ccagtgtgaa
     2161 atactccatc gactgaccac gggaaggggt aagcgcagca ccctggctct tatgattggt
     2221 gcggctgatc gccgcgagtt ccccatagct tttcccgaca atcgggttga atccgccgac
     2281 atcgatcttc atctggtctt cgctcgttgt gttgaagttg cggccaaaat tgaacgagtt
     2341 ccacaggacc cgcctggccc gccacggctt gacgccgtat ttgaattgtt ccggaaaacg
     2401 gttcggatcc gccgccgcgt cgaaagcttc gtgcgccaac agtgccgaag ccgaatggtg
     2461 cccgtgcccg gcccggctgt cgggcggaaa acgggtgatg atcacatccg gctgatattt
     2521 gcggatgacc cacaccacgt cactcaggat cttgtcctgg tcccaggtct tgagcgcttc
     2581 ttccgtcgat ttcgaaaagc cgaagtcata agcccgcgta aagaactgct cggcgccgtc
     2641 cacccgccgc gccgccatca tctcctgcgt cctgatcaat ccgagctcaa ctccttgctc
     2701 atcgccgatc aggttctgcc cgccatctcc tctcgtcatc gacaaatagg cggtcctata
     2761 taaccggccg tccgccatat acgcgatcag tcttgaattt tcatcatccg gatgagcggc
     2821 gatatacagg accgaaccga gtacattcag cttcttgatc cgaaggtata tatccgccga
     2881 ggtcaccgtc gccggtccct ggccgcgcac gcctgcggcg aaggccaatc cggccagaaa
     2941 aaagccaatt accttattca tagtcgcaag ataaaccctt cccccgatcc ctgctttacc
     3001 gctcgcccaa attggcgcct gcgccattca ctgccctacc aggaataccg cgttgcaaaa
     3061 gagcagcttc ccgttttccc agaaactgcg gaagagcgga tcgtccgcca tgcagatcac
     3121 attcccgcgg ccaagattcc ggaccccaaa gatcaagccc tcctttagcc gttccttcgc
     3181 tttattgccc gtgaaaccgc tgatatagcc atccttcctg atcactccta cattccatcc
     3241 ttcgcgcatg aactcataca ccttgtcatc ctgctttaaa gtatagtaat ggtcgggata
     3301 accgaaagcc aatggatggg tgtcgtcaag ctcgatccgg tagatcgccc cccggacgct
     3361 tgtctccgct tcctcgcgct cccggtcgcc ataccggcgg aggagcgcat cctcgtccac
     3421 ccggccgctg tcttccgtgg cctgcttttc tgcatcctcg cctttttcct tgatgcccca
     3481 gtcccccttc gccagttgcc cgacggcatc ctgcatggcg atcagccgcc cgccttcgtg
     3541 gacccaactc cttaggccct cttctacggt cttgtcctcc agcaaccggt agttaccgtt
     3601 tggcaggatc agcacgtcga agtccttcca tgatgcctgg cgcacgtccg aggcattgat
     3661 cagggtgacc ggataatgca gctcctgctc gaaaagatgc catacttcgc cggcctcctg
     3721 cgagctgacc tggtcgcccg agagcatggc cactcttggt gcatgcacca tcctcaccag
     3781 gtcgctgccg aaatccggcc ctttctcgac aaacccggac gtcatcgttg acatatttac
     3841 cccggttgtc cgcgcacact ccacgacagt ctgccgccat ttcgggcccg cggcgccgtt
     3901 acccgccgat gtgatcagga gggtgccccg gtcgtagctc gccccgccgg cctggaaagg
     3961 ccgctcggcg taccgaacct ttatgccttt ttgcagcacc tggctcagga atttcgcgct
     4021 gcttagtccc gaccaccgga ccgcgtatgc atagtcgccc tcttccgcgt cccggtaaac
     4081 cgccgagtct gaagctgcag cgccggcgcc gctcacatag ctgccgagtc catatgcctg
     4141 aaggccatac acaaacggga tggaccatgc ggtaatatcg taagtaatgg agtcactgaa
     4201 cctcgactgc cgctccagca ggaccctcag caggttcgac ttcggctggt tggcgttgat
     4261 cacgatatct cccgggactg agcggaattg ggacatccgg cccgtctcgt aattcaagcc
     4321 gaggtacgtc gcagccggcg cttccgacca caggatgcga ttgcggtcca gcaatccttt
     4381 caggcgccgc aaccggtcgc cgaacgaatc gctccggatg aaatacgcct tgaactcccc
     4441 tcccggccgg ttcaccgcat ccacaaaata ctgatggaag gccttcaaca actggccggc
     4501 atgcagggaa gccgtctcga tcgtcgacat gcccgtgata aagtgatgct ccaggcgatc
     4561 ggcgagccgg agggtatccc cgtcattcgt ccggaccgcc aggcccgcgc cgatcccgcc
     4621 ctgctcatac gtcatgccga ctgcgccgtt atacgtcggg taggtatccc cgtaactggg
     4681 atagaacaga tcgtattcct gccttgtata gaacagccag ccgctccggt cgaaatattt
     4741 cgcattattt tttccgatca acacctggaa ttggcgctgc cagggtgtga taacatcgtg
     4801 ataaggttcc gcggctggcg caaaatagta gggactgttc cagcctt
//
LOCUS       DATGKE010000312         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_726918, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000312 DATGKE010000000
VERSION     DATGKE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726918"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VKU83_05345"
     CDS             complement(<1..526)
                     /locus_tag="VKU83_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLI93008.1"
                     /translation="MFKDRIDAGEILARQLERFKFMPGVILAVPRGGIPIGWVVATRT
                     GLPLDLLMTKKIGHPQNREYAIGAVGMTDSYILPHKGISSSYIKTETEKLRKMLTEMH
                     RLFMPDSPPVKLRNKTVIIIDDGAATGNTLLSAIQILRKEEPARIIVAIPVASVSAAR
                     KLRVAADELVCPLIA"
     gene            complement(546..836)
                     /locus_tag="VKU83_05350"
     CDS             complement(546..836)
                     /locus_tag="VKU83_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLI93009.1"
                     /translation="MEAYNFCQSCGMPMDPEDCRGTEQDGTKSTKYCKFCYRNGEFVD
                     PGMTLEHMKIHARTKLEEMHLPESVIQQAILTLPMLGRWLGSVRRISGNHSL"
     gene            complement(909..1460)
                     /locus_tag="VKU83_05355"
     CDS             complement(909..1460)
                     /locus_tag="VKU83_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HLI93010.1"
                     /translation="MSMITDFRQVIRYWWIFIFLGTILAAFGLSIMAFPVASFRALSI
                     FFEIAIFVNGVLEISFALSNYRRVPGWGWHFSGGLLELAIAGLLLFNPALAAASLSLF
                     VGFWLMFRSISIIGRCFDLPTIWPEKAWMLLLGLAGLVFSFFILYNPQFGTWTLDLWA
                     ALALISIGLFYFFLGVHLKNNPG"
     gene            1614..2951
                     /locus_tag="VKU83_05360"
     CDS             1614..2951
                     /locus_tag="VKU83_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HLI93011.1"
                     /translation="MPGYFTKFPPFSKEEAVAAIKQWAYPLQSKASLQPLMDRIGDAR
                     IVMLGEASHGTHEYYMWRTHISRRLIEEKGFNFIAVEGDWPDCYRLNRFVKGYDADDK
                     GAFKVLQGFDRWPTWMWANWEIVALADWLGKHNKGLAANRRVGFYGLDVYSLWESMES
                     IMKYLEKTDPLAWKVARDAFRCFEPYRGEEGVSYGRAASLVPDLCQTEVIELLKEIQR
                     KLPTYDSDHENVFSTEQNALIAVDAERYYRAMIEGGPHSWNVRDRHMADTLDRLLTFH
                     GESSKAIVWAHNTHVGDARATDMADEGMYNIGELARIEHHEKGVVLIGFGSYAGSVIA
                     GGSWGAPMRVIQMPEAKMDSWEWLLRQAGTENKLLLMDDFATSDMLMEHHIGHRAVGV
                     VYNPAYERYGNYVPSILPLRYDAFVYLDETKALHPLHIAPQGHQVPETYPFGV"
     gene            complement(2962..>3421)
                     /locus_tag="VKU83_05365"
     CDS             complement(2962..>3421)
                     /locus_tag="VKU83_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93012.1"
                     /translation="SADQPNAYMEEILRHGESPVLLLPDGSGLPNQLVMAYDGSASSV
                     HAIRQFVYLFPELCDRPVTVVNLVENPAGPMPDSGLLYEWLVQHFKGVALLQLPMTTE
                     DFLVTWMGDKDAPWLIAGSYGRSGWSQLFAKSFVAESIRRPAYPVFLAHL"
BASE COUNT          855 a          842 c          977 g          747 t
ORIGIN      
        1 cggcgatgag cggacacaca agttcgtcgg cggcgacccg aagtttacgg gcggcactaa
       61 cggatgccac cggaatggcg acgataatgc gcgccggctc ttccttccgg aggatctgga
      121 tagcactgag cagggtgttg ccggtagcgg cgccgtcatc gataatgatc accgtcttgt
      181 tgcgcagctt cacggggggt gaatccggca tgaacagacg gtgcatttcg gttagcatct
      241 ttcgcagctt ttccgtttcg gtcttaatat agctggaaga aatgccttta tgcggcagga
      301 tataactgtc tgtcatgccg actgcgccaa tcgcatattc ccggttttga ggatgaccta
      361 tcttttttgt catcagcaaa tccaaaggca agcctgttct tgtggcaacc acccagccaa
      421 tgggaatgcc tcctctggga accgcgagga tgacaccggg catgaacttg aatcgctcga
      481 gttgcctcgc caggatttcg cctgcatcta ttctgtcctt gaacattggt tgtctatttt
      541 gagtttcata atgaatgatt accggatatt cggcgaacac tgcccagcca gcgcccgagc
      601 atgggcagcg taagaatggc ttgctgaatg acagattcgg gcagatgcat ctcctccagt
      661 ttcgtccggg cgtggatttt catgtgttcc agggtcatgc cgggatcgac aaattccccg
      721 ttccgataac aaaatttgca gtatttggta cttttcgtgc catcctgttc cgttccccgg
      781 caatcctcgg gatccatagg cataccacaa ctctggcaga aattataagc ttccataata
      841 tcctttttga aaaggtacag cgtcattacc gcagaatcta tgacaagtct caacgattgc
      901 gctgatcttt atcccggatt gttttttaag tggacgccga ggaaaaaata aaaaagtccg
      961 atcgaaatga gggccagtgc cgcccagagg tcaagcgtcc atgtaccgaa ttgcggatta
     1021 taaaggatga agaatgaaaa aacgaggccg gcaagaccga ggagcagcat ccaggctttt
     1081 tcgggccaga tggtgggaag atcaaagcag cgcccgataa tggaaatact ccggaacatc
     1141 agccagaatc cgacaaagag cgaaagcgag gcggcagcca gtgcagggtt aaagagaagc
     1201 agtccggcga tcgccaattc gagtaaacct cccgaaaagt gccatcccca accggggact
     1261 ctgcggtaat tgctgagagc aaaggaaatc tcgagcactc cgttgacaaa gatcgcgatt
     1321 tcgaaaaaaa tgctgagcgc cctgaaactc gcaaccggga acgccatgat cgacaggccg
     1381 aaggcggcca ggattgtccc gaggaaaatg aagatccacc aatatcggat gacctgccgg
     1441 aagtcggtga tcatgctcat aatatacctc ctgatgggtt tgtgatcaaa tattaaaact
     1501 atcgaaagca cgcgcccatt taggtgacct ggatcacctg atggactgat tggcatcctt
     1561 ggaacgattg tgcggtttta actaatttta ttatctattt atcagataga ttcatgcctg
     1621 gatatttcac caaattcccg ccttttagca aagaggaagc tgtcgccgct atcaaacagt
     1681 gggcctaccc gctgcaaagc aaagccagcc tgcagccact gatggatcgc atcggtgatg
     1741 cccggatagt aatgctgggt gaggcgagcc acggcacgca cgagtattac atgtggcgaa
     1801 cgcatatttc caggaggctg atcgaagaaa aaggctttaa ttttattgcg gtcgaaggcg
     1861 attggccgga ctgttaccgg cttaaccggt ttgtgaaagg atatgatgcg gacgacaaag
     1921 gggcattcaa ggtcttacag ggatttgacc ggtggcctac ctggatgtgg gctaactggg
     1981 agatcgtggc gcttgcggac tggcttggga agcataataa agggctggcg gcgaacaggc
     2041 gggtcggatt ctatggattg gacgtgtata gcctttggga gagtatggaa agcatcatga
     2101 agtacctgga aaaaacggac ccgttggcct ggaaggtggc tcgcgacgcc ttccgctgtt
     2161 tcgaacccta tcgcggggaa gaaggcgttt catatggacg ggccgccagt ctggttccgg
     2221 acctgtgcca gacagaagtg atcgaattgc tgaaggaaat ccaaaggaag ctgcccactt
     2281 atgactcaga tcacgagaat gtattcagca ccgaacagaa tgcgttgatc gccgtcgacg
     2341 ccgaaagata ttaccgggcc atgatcgagg gcggccctca cagttggaat gtccgggaca
     2401 ggcatatggc ggacaccctg gaccggttat taacattcca cggcgaaagc tccaaagcca
     2461 tcgtttgggc gcacaatacg cacgttggcg atgcgcgggc aaccgatatg gccgacgagg
     2521 ggatgtataa tatcggcgag ttggccagga tcgagcatca tgaaaaggga gtagtgctta
     2581 tcggatttgg atcttatgcc gggtccgtta tcgccggggg cagttgggga gcgcccatgc
     2641 gcgtgattca aatgcctgaa gcaaagatgg acagctggga gtggctgctg cgccaggcgg
     2701 gtacggaaaa caagctgctg ctgatggacg attttgctac cagcgatatg ctgatggagc
     2761 accacatcgg tcaccgcgcg gttggcgtcg tttataaccc ggcatatgaa cgatacggca
     2821 attatgttcc cagcattttg cctttgcggt atgatgcttt tgtctatctg gatgaaacga
     2881 aagcgcttca ccccctccat atcgcgccgc agggccacca ggtacccgag acgtatccgt
     2941 ttggggtctg aaccggccag gctacaaatg agccaggaat acagggtaag cgggcctgcg
     3001 gatggattcg gcaacgaaac tctttgcaaa cagttgcgac cagcccgacc ggccatagga
     3061 tccggcgatg agccagggtg cgtctttatc gcccatccag gtgaccagga aatcttccgt
     3121 tgtcatcgga agctggagca gcgccacgcc tttaaaatgt tggaccagcc attcgtagag
     3181 gaggccggaa tccggcatgg gaccggctgg attctccacc aggttgacga cggttaccgg
     3241 ccggtcgcac agctccggaa agagataaac gaactgccgg atggcatgca ccgaagaagc
     3301 agatccgtca taagccatga caagctgatt cggaaggccc gacccatcag gaaggaggag
     3361 gaccggcgat tctccatggc gcaggatttc ctccatatag gcattgggct ggtcggcgct
     3421 g
//
LOCUS       DATGKE010000313         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_534657, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000313 DATGKE010000000
VERSION     DATGKE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534657"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..2164
                     /locus_tag="VKU83_05370"
     CDS             215..2164
                     /locus_tag="VKU83_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HLI93013.1"
                     /translation="MRYLPLLFLALAGFQGLAQNRPPSTFGKVTAEDFAVNFPGLDTS
                     ADAVVVSDVGVRTFERPYALSTQWDGYLQHTRRVLILKRRGFEAATVVIPLFSFKGRS
                     EEIMDLKAATYNLEGGKVVKTELAKNSVFKEKLSENVMTERFAFPAVREGSIVEYTYK
                     EKSEFGFSNETWIFQGSYPCLWSEYLISIPAAFGYSVVPHSYLPFCIDTTQSEKSDYG
                     AVYGAIRTYHWAIKDIPALKEQPYTTTIRNWLARVEIRPGGLLYNWATLDRQLLRSDH
                     FGADLTAGNSWLDNDIREITAGAADDLSKARRIYAYVRDHFTCNKRPGYTMTTTLKNI
                     YKNRSGSEAELNLLLTAMLDHERLKAAPVLLSTRPNGFINPFVAQPAVLNYVICKLNC
                     GPTSYYLDASDVNLRFNQLPLDCYNDYDFVMDTAGQFLEAALTADSLIEQKKVIVFLS
                     NGDKGGLDGSVQSFPGTAEATEIRRKMKIPDGDKKFEDQLRTGLMAEETVSDVEIDSL
                     KMPDEPLVINYSCHFAIDSASNIFYFTPMLEDRMAENPLKDALRIYPVEMPCSKDLNY
                     ILTMDIPTGFVVEELPKSEKILLNENGGYFEYIVSKDGDQIHFRIRFRLTKANFQPHE
                     YEGLRNFFATILEKENEQIVFKRKE"
     gene            2171..>2406
                     /locus_tag="VKU83_05375"
     CDS             2171..>2406
                     /locus_tag="VKU83_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93014.1"
                     /translation="MKNAAVVVVLVLGSSVAVSAQSYSVALVPDSLMKGARAVMREDE
                     TILEIKSPEKAVFRQRHVFTILNQNADNIGGYDS"
BASE COUNT          635 a          496 c          665 g          610 t
ORIGIN      
        1 tgtcgcctca agcttaataa ggcgaatttc gctcctgacg actacaattc gttgcgggaa
       61 ttctatgcgc tggttgtgaa aaaggaagcg gaagccatcg tgctgaaaaa gaattgagcc
      121 ggaggtgttg actactcgac attgcgggcc ttcttcggtt atattgtgaa gaaggaacag
      181 gagcagattg tgtttaaaaa gataaaataa aattatgcga tacttgcctc ttttattcct
      241 tgcccttgcg gggtttcagg ggctcgcgca gaataggccg ccgtccacat ttggcaaagt
      301 gacggccgag gactttgcgg tcaatttccc ggggctggat acatcggcag acgccgttgt
      361 ggtatcggat gtcggggtca ggaccttcga acggccgtat gctttatcga ctcaatggga
      421 cggttatctg cagcatacga ggcgagtcct gatcctgaaa aggcgtgggt ttgaggctgc
      481 tactgttgtt atcccgttgt ttagtttcaa agggcggagt gaggaaataa tggaccttaa
      541 agctgcaacg tataacctgg aaggcggcaa agtagtgaag acggaattgg ccaagaatag
      601 tgtattcaag gaaaagcttt cggaaaacgt gatgaccgaa cgatttgctt ttccggcggt
      661 ccgtgaagga tcgatcgtgg agtacacgta taaggaaaaa tcggagtttg gttttagcaa
      721 tgagacctgg atcttccaag gctcttatcc ctgcctgtgg agtgaatacc tcataagcat
      781 ccctgcggca tttggctact ccgtggtgcc gcattcctat ttgccctttt gcatcgatac
      841 gacgcaatcg gaaaagagtg attatggggc ggtatatgga gcaatcagaa cgtatcactg
      901 ggcgatcaag gatatcccgg cactgaaaga gcaaccttac acaacgacga tcaggaattg
      961 gttggcacgt gttgagatca ggccgggagg gttactgtat aattgggcaa cgttagaccg
     1021 gcaactgctg cgctctgatc actttggcgc cgatctgaca gctggtaatt cgtggctgga
     1081 taacgatata agggagataa cggcgggagc cgcggatgat ctaagcaaag caaggcggat
     1141 ctatgcctat gtccgggatc attttacctg caacaagcgc ccggggtaca ccatgacgac
     1201 cacactcaag aatatatata agaaccggag tggcagcgag gccgagttga acctgttgtt
     1261 gacggcgatg ctggatcatg agaggttgaa ggctgctcca gtcctgttaa gcacccgacc
     1321 caacggcttc atcaatccgt ttgtggcgca gccggcagtt ttgaactatg tcatttgtaa
     1381 attgaattgc ggtcccacgt cctactatct tgatgcgagc gatgtaaacc tacggttcaa
     1441 ccagctgccg ttagactgct ataacgatta cgattttgtg atggacacag ctggacaatt
     1501 ccttgaagcc gccctgactg cagattcctt gatagaacaa aaaaaagtga ttgtctttct
     1561 ttccaatggg gataaaggcg gcctggatgg ttcagttcaa agttttccgg ggacggcgga
     1621 ggcgacggag atcagaagaa aaatgaaaat cccggatgga gacaaaaaat tcgaggatca
     1681 actaagaacc gggctgatgg cggaagaaac cgtctccgat gtggagatcg attcgctgaa
     1741 aatgcccgac gaaccgctgg ttattaatta ttcctgtcat ttcgccatcg acagtgcttc
     1801 caacattttc tatttcacgc ctatgctgga ggacaggatg gcggaaaatc ccctgaagga
     1861 cgcgttgcga atatatcccg tcgagatgcc gtgttcaaag gatttgaatt atattctgac
     1921 catggatatt cctaccggct ttgttgtgga agagctacca aaatcggaaa agatattatt
     1981 gaatgaaaat gggggttatt tcgaatacat tgtatcgaag gatggtgacc agattcattt
     2041 tcggattcgg ttccggctga caaaggcgaa tttccagccg catgagtatg aaggattgcg
     2101 caattttttt gcgaccattt tagagaagga aaatgaacag atcgttttta aaagaaaaga
     2161 gtaattttct atgaaaaatg cggcagtcgt cgtcgttttg gttcttgggt cttcggtagc
     2221 tgtttcggct caatcgtaca gtgttgcatt ggtcccggat tcactgatga aaggcgcccg
     2281 ggcagtcatg cgcgaggatg agacgatcct ggagataaaa tcgccggaga aggcggtttt
     2341 caggcagcgg catgttttta cgatactgaa tcaaaatgcg gacaatattg ggggctatga
     2401 tagctg
//
LOCUS       DATGKE010000314         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_206290, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000314 DATGKE010000000
VERSION     DATGKE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206290"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..139)
                     /locus_tag="VKU83_05380"
     tRNA            complement(68..139)
                     /locus_tag="VKU83_05380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Arg,seq:cct)
     gene            232..684
                     /locus_tag="VKU83_05385"
     CDS             232..684
                     /locus_tag="VKU83_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI93015.1"
                     /translation="MDRMIRAWVLTAVMAGFGLGARAQAPHFSHQTIYVTDLNRAANF
                     YEKVLELRRISEPFHDGRHVWFRVSEHGQLHVVSGAREDVPHDINIHMAFSVPSVEAF
                     AKHLDAMHVRYGNWAQNDKTPQLRPDKVKQIYLQDPDGYWIEINDDKF"
     gene            743..1393
                     /locus_tag="VKU83_05390"
     CDS             743..1393
                     /locus_tag="VKU83_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93016.1"
                     /translation="MKFYNIIIKYRLQIAALMLVIGVATNLYTGFWPAFLPYFIALIL
                     VFGYFFFGPLRLIQEHMEAGNMDAAEKVLNSVKFPGLLYKPIRSVYYTLKGNIAMMKQ
                     DFTSAEKNMKKGLDLGMPMKEAEGASLLQMGMLSMQKNDLRQAESYIRQAIRKGLPDK
                     ENQAAAFLQLCTVMMTKREFRAAKDFFRKAKALKPTTPQIVSQIKEIERYISRIPG"
     gene            complement(1432..1827)
                     /locus_tag="VKU83_05395"
     CDS             complement(1432..1827)
                     /locus_tag="VKU83_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93017.1"
                     /translation="MELTQPVIFVVEDNIIYQGLIAKELESLSNNLHFYTNGESCIMD
                     LNKRPSVIVLDYNLDGQMNGLDTLQRVRDFDPNVYVILFSSQKGLNTKEVFLQYGSFD
                     FLEKNSLSLRTLRQMVDCVTSAGGKTVSN"
     gene            complement(1972..>3652)
                     /locus_tag="VKU83_05400"
     CDS             complement(1972..>3652)
                     /locus_tag="VKU83_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI93018.1"
                     /translation="GEEIYLLYKNYRREEPGAEPYVIGFSQDITDRIKMEKELRFTKQ
                     LTDDIARAKESFLAHMSHEIRTPMNGILGIASLLSKTQLEPQQRNYLTLIQDSANNLL
                     VIVNDILDLEKIVAGKLQIESIPFKLVDKIATTIQSFIYRAEEKELGLIFQNFIPADL
                     VVKGDPYRLSQVLNNILSNALKFTEVGHITIATSIRENNGEEVVAEITISDTGIGIAR
                     ERLATIFEPFEQADATISRKYGGTGLGLAISKNMIEMQNGDLLVESEEGKGSTFTILI
                     PYQLSIETTADNESRQEIDYKSLGPRRVLVAEDVELNQFLARHILESWDFQVVIAGNG
                     LEAIEHLGKDSFDFILMDVQMPEMDGIEATHHIRSLADPVKANIPIIALTANALKGDS
                     EKYIAAGMTDYLAKPFDEERLFRVISRNLAPTPCCSPIKEDTFKNNHTIMSAANPRLY
                     DLTMVQSVSGGDEGFIKKMVALFVETVPQNLQELKNALAAKNWEMVAKTAHKLKSTID
                     SMGIKSIRDQIRTVEANAKQGESLETIPALVETIDSTITDCIVQLQAEVLN"
BASE COUNT          857 a          909 c          979 g          907 t
ORIGIN      
        1 caggcaacta aaaatctata gttgttctca agcaattgaa aatcaataca aaaattgcag
       61 aaaggtgtag tcccgacagg aatcgaacct gtatcaaaag tttaggaaac ttctattcta
      121 tccattgaac tacgggacca aaacggaagg gccgcaaaaa taacacaaat tttttttagc
      181 cggtgcaatt ccgcggaatt ccttactttg actgccttaa aatcaacgaa aatggatcga
      241 atgatcaggg catgggtact gactgcggtg atggcgggct tcggccttgg cgcccgggcg
      301 caggccccgc atttcagtca ccagaccatc tacgtaaccg acttgaaccg cgcggcgaac
      361 ttttatgaaa aagtgctgga actgagacgg atctccgagc ccttccatga cggccggcat
      421 gtttggttcc gcgtgtccga gcatggacag ctccatgtag tctccggcgc cagggaggac
      481 gtgccgcacg atatcaatat tcatatggct ttcagcgtac cgtcggtcga ggcatttgcg
      541 aaacaccttg acgccatgca cgtcaggtac gggaattggg cccagaacga taaaacgcca
      601 caactcaggc cggacaaggt gaagcagatc tacctccagg acccggatgg ctactggatt
      661 gagatcaacg acgataaatt ttagcggatg cggcggggcg gggccgaacc cgtatatttg
      721 cagtcccgac taaaaaggac aaatgaagtt ctacaatatt atcattaaat atcgcctgca
      781 gatcgccgcc ctgatgctcg ttatcggcgt ggcgaccaat ctgtataccg gtttttggcc
      841 ggccttcctc ccttatttca ttgcgcttat ccttgttttc ggctatttct ttttcgggcc
      901 gctgcggctg atccaggaac atatggaggc gggcaatatg gacgccgcgg agaaagtgct
      961 caactccgtt aaattcccgg ggttgctcta taagcctatc cgttcggtct attacacgct
     1021 taagggcaac atcgccatga tgaagcagga tttcacgtcg gcggaaaaga acatgaaaaa
     1081 gggattagac ctcgggatgc cgatgaagga agccgaaggt gcgagcctgc tgcagatggg
     1141 catgctgtcc atgcagaaga acgacctcag gcaggccgaa agctatatcc ggcaggcgat
     1201 acgcaagggg ctccctgaca aggagaacca ggccgccgca ttcctgcagt tatgtaccgt
     1261 catgatgacg aagcgcgagt tccgggcggc gaaggatttt ttccgcaagg ccaaggcgct
     1321 caagccgacg acgccgcaga tcgtgagtca gattaaagag attgaaagat atatctcgag
     1381 aataccggga tgaagtgagg ggcggtccgt ttttggatca ggtcgggtgc ttcagttaga
     1441 gaccgtcttg ccgccggccg aggtaacgca atccaccatt tgccgaagag tgcgtagaga
     1501 aaggctattt ttttccagga aatcaaaact gccgtattgc aggaacacct ctttggtgtt
     1561 tagtcccttt tggctcgaaa acaggatcac gtaaacattc gggtcgaagt cacgtaccct
     1621 ttgaagggtg tcgaggccgt tcatttgtcc gtcaagatta taatcgagca caataacgga
     1681 tggccgcttg ttcaagtcca tgatgcaaga ctcgccgttc gtataaaagt ggagattgtt
     1741 gctgagcgat tccagctcct tggcgataag gccctggtaa ataatattat cttccacaac
     1801 gaagataaca ggttgtgtta attccattgg ttgtcgatct ggtgcgagga taaaacccgg
     1861 aaaatggtgc caaaaaaatt aatgctgtaa aggcctgcaa atcaacacaa cgcgaaaacc
     1921 cgcacggcgg gtttactctc gaattgggaa aaagttgcta tttgttggaa atcaattaag
     1981 tacttcagct tgtagctgga cgatgcagtc cgtgatcgta ctgtcgatcg tctcgacgag
     2041 ggccgggatc gtttccaggg actcgccctg cttggcgttg gcttcaacgg tgcggatctg
     2101 gtcgcggatg gacttgatgc ccatcgaatc aatcgtggac ttgagcttat gagcggtctt
     2161 tgcgaccatc tcccagtttt tcgccgcaag cgcgttctta agctcctgca aattctgggg
     2221 caccgtctct acaaaaagcg ctaccatctt tttaatgaat ccctcatcac cgccggagac
     2281 ggattggacc attgtcaaat cgtataatct tggattcgca gctgacatga ttgtatggtt
     2341 gtttttaaag gtgtcttctt ttattggact acagcatggg gtcggtgcga ggttgcgtga
     2401 aatgacacgg aacaaacgct cctcgtcaaa gggtttcgcg aggtagtcgg tcatgcccgc
     2461 cgcaatgtat ttttcactgt cgcctttcag cgcatttgcg gtcagggcga taatgggaat
     2521 attggctttg acggggtcgg cgaggctgcg gatgtgatgc gttgcttcta tcccatccat
     2581 ttccggcatc tgcacgtcca tcagaatgaa gtcgaacgag tcttttccaa ggtgctcgat
     2641 cgcctccagc ccgtttcccg ctatgaccac ctggaaatcc caggattcaa ggatatggcg
     2701 ggcgaggaac tggttgagct ccacgtcttc cgcaaccagc acccttcggg gcccaaggct
     2761 cttgtaatct atctcctgcc ggctctcatt atcggccgtt gtttcaatgc ttagctgata
     2821 cgggatcaaa atggtgaatg tcgatccctt cccctcctcg ctttccacaa ggagatcgcc
     2881 gttctgcatc tcgatcatat ttttggagat cgccaggcca agaccggtac ctccgtattt
     2941 ccgggagatg gtcgcatcgg cctgttcgaa cggctcgaag atggtggcca ggcgttcccg
     3001 ggcgatgccg atgcccgtgt cgctgatggt gatctccgcc acgacttcct cgccgttgtt
     3061 ctcgcggatc gacgtagcga tcgtaatgtg gccgacttcc gtaaacttca gggcattgct
     3121 caggatattg ttcaacacct ggctaagccg gtacggatcg cccttcacga cgaggtccgc
     3181 cgggataaag ttctgaaaaa tgagacccaa ttctttctcc tccgcacgat aaataaagga
     3241 ctgtatcgtg gtggcgatct tgtctactaa tttgaagggt atcgattcga tctgcaattt
     3301 gccggcgaca attttttcga ggtccaaaat atcgtttacg atcacgagca ggttgttcgc
     3361 cgagtcctgg atcaaggtca ggtaattacg ttgctgtggt tccagctggg tcttgctgag
     3421 caggctggcg atcccgagga tgccgttcat gggtgtccgg atctcatggc tcatgtgggc
     3481 caggaacgat tctttggccc gggcgatatc gtcggtcagc tgtttggtga accgaagctc
     3541 cttttccatt ttgatgcggt cggtgatgtc ctgggaaaag cctatcacat aaggctcggc
     3601 gccgggctcc tcccgccggt agttcttata gagaagatag atctcctcgc cg
//
LOCUS       DATGKE010000315         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_158355, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000315 DATGKE010000000
VERSION     DATGKE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158355"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VKU83_05405"
     CDS             complement(<1..193)
                     /locus_tag="VKU83_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLI93019.1"
                     /translation="MNSSKVLVGFLVGAIVGGALGVLLAPDKGSETRRRIIEKGSDLG
                     DCITEFGGTIKDKFSQMVNG"
     gene            432..1925
                     /locus_tag="VKU83_05410"
     CDS             432..1925
                     /locus_tag="VKU83_05410"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HLI93020.1"
                     /translation="MQNGTILQYFHWYYPADGSLWTKLRAEAQQLAEMGFTAVWLPPA
                     SKGTNGTFSVGYDIYDLWDLGEFDQKNSVRTKYGTKQEYLDAIRAVHAAGMQVYVDVV
                     LNHKAGGDKCEVVKVRKADPEDRRKFVSDPFEIEAYTKFTFPGRKGKYSEFVWDYRCF
                     TGVDYDNRTKERAIYNILNDFGDSWEEVVDDEKGNYDYLMYDDIEFRNEAVREELKRW
                     GEWYFKLTGFDGIRLDAVKHMSPAFVNEWVDAMRRSAGKDLFIVGEYWAPGNLPLLMK
                     YIEATEGRLSLFDASLHHNIQSASKCGKEFDLTTIFNETLVAARPELAVTVVGNHDTQ
                     PLQSLEAPVEDWFKPLAHALLLLREKGYPCVFYPDLYSARYKDTGHDGNECEIFLAAV
                     PQIDKLMVARKRFAYGVERDCLDHPNCIGWTREGDDDHPGSGCAVLLSNGDEGSKTME
                     IGRRHAGKTFVDWLGGRSESVVIDQEGQGAFKVNGGSVSVWIQADQA"
     gene            2007..>3228
                     /locus_tag="VKU83_05415"
     CDS             2007..>3228
                     /locus_tag="VKU83_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HLI93021.1"
                     /translation="MKTAIRQFAFDLRVRVGYGTAFFLLLSSYLLTLYANSKLLNEAV
                     LVDHSNKIIIETEALGSGIKDAESGFRGYLLMDDPRFLYPYKESKRATDSIYGALLAE
                     TSRDHEEHSDLATLRTLTLKKYEVMDAGLAEFAKNPVKTADSVRLQRYFSLEIMNHIK
                     TVISQIQATERQRLRDRLAGMASRYHTLNNVILLSLGIAIIMALLGYYAYSNENRARR
                     EADRRVTEYQGQLHDRIGELDKANKELIRMRSIEKFAATGRIARTIAHEVRNPLTNIN
                     LAVEQLQADLGGHLDGVRPGNSETMLQLINRNSARINLLITDLLNSTKFTDLTYAKAS
                     VNRLLDETLELAKDRIHLKGISIQKNYAGGLPDIAVDIERIKIAFLNIVVNAVEAMEA
                     NKGILRLSTFSRENK"
BASE COUNT          795 a          899 c          857 g          677 t
ORIGIN      
        1 cgccgttcac catttgactg aatttatcct tgatggtccc gccgaattcc gtaatgcagt
       61 ctccgagatc gcttcccttt tcaattatcc tccggcgcgt ctctgaaccc ttatccggag
      121 ccaacaggac gcctagggcg ccgccgacga tcgcgcctac caaaaagccg actaatactt
      181 ttgaactgtt catcttgtct gtttttttgc ggttaacaat aatgatctac tgacacccat
      241 gaaattcctt gccaatctgc ctttatgcgg tagaaatatt ttgtcagcgc ctaactgaga
      301 aaaatattct acaaattgag caagcagtct gggccggcgg ccactgccca attgaaaaac
      361 gccggcgtcc aagatggtat cattatggtt atagcggaat cgtcgatcct tcggcttaaa
      421 acgatttcaa catgcaaaac gggaccatcc tgcaatactt tcattggtac tatcctgccg
      481 acggcagcct ttggacaaaa ctccgggctg aagcgcagca gctggcggaa atgggtttta
      541 cggccgtctg gctgccccca gccagcaagg gaacgaacgg aacgttttcc gtaggttacg
      601 acatttatga tctctgggac ctcggcgaat tcgatcagaa gaattccgtg cgaaccaagt
      661 acggcacgaa gcaggaatac ctggacgcga tccgggccgt gcacgcggcg ggcatgcagg
      721 tttacgtcga tgtcgtgctc aaccataagg ccggcggcga caaatgcgag gtcgtcaaag
      781 tccggaaggc cgatccggag gaccggagaa aattcgtcag cgacccattc gaaattgaag
      841 cctacaccaa attcactttt cccggccgga aagggaagta ctcggagttc gtctgggatt
      901 accgttgttt cacgggggtc gactatgaca accgcacaaa ggaaagggcc atctacaata
      961 tcctcaacga tttcggcgat agctgggaag aagttgtcga cgacgaaaag ggcaattatg
     1021 actacctgat gtacgacgat atcgaattcc ggaacgaagc agtaagggaa gagctcaaac
     1081 gctggggcga gtggtatttc aagctgaccg gtttcgacgg catcaggctg gatgcggtga
     1141 agcatatgtc gcctgcgttc gtgaacgaat gggtcgatgc gatgcgccgc agtgccggaa
     1201 aggacctatt catagtcggt gaatattggg cgccgggtaa tctgccgctc ctgatgaaat
     1261 atatcgaagc gacggaaggc cggctgtccc tcttcgatgc gtccctgcat cataatatcc
     1321 agtccgcctc aaagtgcggc aaggaattcg accttacgac catctttaat gagaccctcg
     1381 tggcggcccg gccggaactg gcggttaccg tcgtcggcaa ccacgacacc caaccactcc
     1441 agtccctcga agcaccagtg gaggattggt tcaagcccct ggcgcacgcc ttgttattgt
     1501 tacgtgaaaa aggatatcca tgtgtttttt atcccgatct ttacagtgcc cggtacaagg
     1561 ataccggaca tgacggcaat gaatgcgaga tcttccttgc ggcggtgccc caaatcgaca
     1621 agttgatggt tgcgaggaaa cggtttgcgt acggcgtcga gcgcgattgc ctcgaccatc
     1681 ccaattgcat cggctggacc cgggaaggcg atgacgacca tcccggctcg ggttgcgctg
     1741 tgctcctcag caacggcgac gaagggtcca agaccatgga gatcggccgg cgccatgccg
     1801 gaaaaacctt tgtcgactgg ctcggcggcc gcagtgaaag cgtggtcatc gatcaggaag
     1861 gtcaaggagc gttcaaagtt aatggcggtt cggtttccgt ttggatccag gcggatcaag
     1921 cctgaaccgc cccaccctcc acctgcggga gactgcccct gtctcccgga cccacaccga
     1981 taccaaaatg cgtaacagtc gagcgtatga aaactgctat ccgacaattt gccttcgatc
     2041 tccgcgttcg cgtaggttac gggacagcat tcttcctgct gttgtcgtcg tacctgctca
     2101 cgctctatgc caactcgaaa ttgttgaacg aggccgttct cgtcgatcac tcgaataaga
     2161 tcattatcga aacggaagcc ctcggttcgg ggatcaaaga cgccgaatca gggtttcgcg
     2221 gctatcttct catggatgac ccccgcttcc tttatcctta taaagaaagc aaacgggcca
     2281 ccgactcgat ttacggggcc ttgctcgccg aaacgtcacg ggaccatgaa gaacattcgg
     2341 atctggcgac actccggacg ctcaccctta agaaatatga agtgatggac gccggcctcg
     2401 cggaattcgc caaaaacccg gttaaaacgg ctgatagcgt gcgtttgcag cgatattttt
     2461 cactggaaat catgaaccac atcaagactg tcatcagcca gatccaggca accgaacggc
     2521 agcggctccg cgacaggctg gcgggcatgg ccagccgcta ccatacgctc aataacgtca
     2581 tcctcctgtc cctgggcatc gccattatca tggccctgtt ggggtactat gcctactcca
     2641 atgagaaccg ggcccgccgt gaagcggatc gccgggtaac agaataccag ggacaattgc
     2701 acgaccggat cggcgagctg gacaaagcaa ataaggaact gatccggatg cgaagcatcg
     2761 aaaaatttgc cgcaaccggg cggatcgcca ggaccatcgc ccacgaagtc cggaacccgc
     2821 tcaccaacat caatctggcg gtggagcaac tgcaggctga tctgggcggg catctggacg
     2881 gggttcgtcc ggggaatagc gagacgatgc tgcaattgat caaccgcaac agcgcccgca
     2941 tcaacctgct gatcacggac ttgctgaatt cgacaaaatt taccgacctc acctacgcga
     3001 aggcgtccgt caaccggctg ctcgacgaaa ccctggaact ggcaaaggac cgcatccatc
     3061 ttaagggcat cagcatccag aaaaactatg ccggcggatt acccgatatc gcggtcgaca
     3121 ttgagcgcat caagatcgcc ttcctgaata tcgtagtcaa cgctgttgaa gccatggaag
     3181 ccaataaggg tatcctcagg ctgtccacct tctctcggga aaacaaat
//
LOCUS       DATGKE010000316         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_640815, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000316 DATGKE010000000
VERSION     DATGKE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640815"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VKU83_05420"
     CDS             complement(<1..246)
                     /locus_tag="VKU83_05420"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HLI93022.1"
                     /translation="MEKELKVFKLQGRVQHYAWGGTDFIPALLSVPNPGRRPFAEYWM
                     GAHEHAPSMLEIAGGDLRPAGNADASIGLGEYIAQRPE"
     gene            288..644
                     /locus_tag="VKU83_05425"
     CDS             288..644
                     /locus_tag="VKU83_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLI93023.1"
                     /translation="MAAHIGLGKKGEDLAVEWLGANGFHILHRNWRHGRYEIDVIAGR
                     EQVLHIVEVKTRRSGLFGPPETGVNRKKFRNLLQASSRWLANHPGHTRVQYDVLSITL
                     HDNNSPEYLLFEDVFL"
     gene            complement(639..1151)
                     /locus_tag="VKU83_05430"
     CDS             complement(639..1151)
                     /locus_tag="VKU83_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLI93024.1"
                     /translation="MAITVHLRAFKLQVSHNRRRMRYFLTRLEKNVPRGLQHAAANAD
                     QAAWQRTDCRDCANCCKTMSPTYTKNDISRISAHLGMSEKAFKDKWLYKDKTGDWMNV
                     SQPCQFLDTATNLCKIYEVRPGDCAGFPHHNKKLGPDYMHVFKQNVEFCPVTYRMVQA
                     LMENLSGKGS"
     gene            1213..2115
                     /gene="xerD"
                     /locus_tag="VKU83_05435"
     CDS             1213..2115
                     /gene="xerD"
                     /locus_tag="VKU83_05435"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLI93025.1"
                     /translation="MWEAYKKGYKAWLQLERSLSDNSIEAYLRDLDKFTQYLQTSGDL
                     KGPDAIALKDLQGFLRWVAEIGLNAASQARILSGIRSFYKYCFIEQIVRQDPTTLLDA
                     PKLKRALPDVLSFEEIEKIIAQIDLSKPEGHRNKAILETMYSCGLRVTEAVNLRISCL
                     YPEEGYIRVTGKGDKERLIPIGSSAIGEIRVYQHQVRAHLTIQPGHEDILFLNRRGSR
                     LSRVMIFLVLKDLVRQAGINKNISPHTFRHSFATHLVEGGADLRAVQEMLGHESITTT
                     EIYTHLDREYLRETLHRYHPAFRH"
     gene            2205..2840
                     /locus_tag="VKU83_05440"
     CDS             2205..2840
                     /locus_tag="VKU83_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI93026.1"
                     /translation="MTFLKTISPNENGSPATDQELLDGYRKAGDLGILAQLYQRYLDL
                     LYGVCLKYFNDREMAKDAVMDIFEELPPKLARHEVGHFKAWLYTVAKNHCLMQLRASG
                     RLRLNSLDPDLVQMPEETHLNDKLDQEARLDQLSDCISTLSADQKTVVSLFYLENKCY
                     KEIECATGFEWNRVRSLIQNGRRNLRLCMERQEGPPTRAGDSRPAPAPEKK"
     gene            2837..>3213
                     /locus_tag="VKU83_05445"
     CDS             2837..>3213
                     /locus_tag="VKU83_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93027.1"
                     /translation="MRKRHHTAHYSASDIQKYLRGEMSAAEMHTLERAALEDPFLADA
                     IEGLAFHGGQVSDSGLQQDLAQLQERLTSRTNARRRQHPFFLRPVSLAAAVLLLLGIG
                     YSYFIFFTARKPRMTPSAHVAYTA"
BASE COUNT          747 a          970 c          805 g          691 t
ORIGIN      
        1 ctccggccgc tgcgctatat attcacccaa gccaatactt gcatccgcgt tgccggccgg
       61 ccgcaaatca ccgcccgcaa tctccaacat ggacggcgca tgctcgtgcg cacccatcca
      121 atactccgca aacggcctgc ggccgggatt cggcactgac aacagcgccg ggataaaatc
      181 agtgccaccc cacgcataat gttgaaccct tccctgcagt ttgaaaactt taagctcctt
      241 ttccatattc ttttccgaac ttccgggcaa gttacaacaa aaaagaaatg gcggcccata
      301 tcggtctggg taagaaaggg gaggacctcg cggtcgaatg gctcggcgcc aacggctttc
      361 acatccttca ccgtaactgg cggcacggcc ggtatgagat cgatgtcatc gcgggccggg
      421 aacaggtgct gcatatcgtg gaggtaaaaa cgcggaggtc cggcctcttt ggtccgcctg
      481 aaacgggcgt caatcgcaaa aaattccgca atcttttaca ggcgtcttcc cgttggcttg
      541 ctaaccatcc cgggcatacg cgagtccaat acgacgtgct gtccatcacg cttcatgaca
      601 ataattcgcc tgaatatttg ctgtttgaag acgtattcct atgatccctt gcccgagagg
      661 ttttccatca acgcctgaac catccggtac gtgaccgggc aaaactccac attctgcttg
      721 aacacatgca tataatccgg gcccagtttt ttattgtgat gcgggaagcc ggcgcaatcc
      781 cccggccgaa cctcataaat cttacacagg ttcgtcgccg tgtccaggaa ctggcacggc
      841 tggctgacat tcatccagtc gccggtcttg tccttataca gccatttgtc cttgaacgct
      901 ttttcgctca tcccaaggtg cgccgatatc ctcgagatgt cattcttagt ataagtaggc
      961 gacatcgtct tgcagcaatt cgcgcaatcc cggcaatcgg tacgctgcca tgctgcctgg
     1021 tcggcgttcg cagccgcatg ctgaaggccg cgcggtacat ttttttccaa ccgggtcagg
     1081 aaataacgca tccgccggcg attgtggctg acttgcagtt tgaaagcgcg taaatgaacc
     1141 gtgatagcca tggatgcaaa tatgccgcaa tcaatgggag ttacaaagta ttttttcggg
     1201 taacttcgct gaatgtggga agcgtacaag aaaggataca aggcctggct acaattggaa
     1261 agatcgctgt cggataactc tatcgaagcc tatttgcgcg acctcgacaa gtttacccag
     1321 tacctgcaaa cgagcggcga cctaaaaggc cccgacgcga tcgccctgaa agacctccag
     1381 ggttttttgc gttgggtcgc agaaatcggg ctcaacgcgg cctcccaggc ccgtatccta
     1441 tccggaatcc gctccttcta caagtactgc tttattgagc agatcgtccg ccaggatccg
     1501 accacattgc tggacgcccc gaaattaaag cgcgccctgc cggacgtcct gagcttcgaa
     1561 gagatcgaaa agatcatcgc ccagatcgac ctcagcaagc ccgaagggca ccggaataaa
     1621 gctatcctgg aaacgatgta cagctgtggg cttcgggtaa ccgaagctgt caatctccgc
     1681 atttcctgcc tttatccgga agagggctac atccgcgtga ccggcaaagg cgacaaggaa
     1741 aggctgatcc ctattgggag tagcgccatc ggggagatcc gcgtctacca gcaccaggtc
     1801 agagcacacc tgaccatcca acccggtcac gaagacattc tcttcctcaa ccgccgggga
     1861 agccgcctca gccgggtcat gatctttctc gtcctcaaag acctggtacg gcaggccggc
     1921 attaataaaa acatctcgcc gcataccttt cgccattcct ttgcgaccca cctcgtcgaa
     1981 ggcggcgcgg acctgcgcgc ggttcaggaa atgctgggtc atgagagcat caccacgacc
     2041 gagatctata cgcatcttga ccgggagtac ctccgcgaga ccctacaccg gtatcatccc
     2101 gccttccggc attgatccct ggcactttca tccgtccatc ccggcagctc ccatttccgg
     2161 taaattttgt ctattttggc agccggtcac ccgtttagca gcctttgact tttctaaaaa
     2221 cgatatcgcc gaacgaaaac ggctctcctg caaccgacca ggaactgctc gacggctacc
     2281 gaaaggccgg cgaccttgga atcctcgcgc aactttacca gcgctacctt gacctcctct
     2341 atggcgtttg tcttaagtac ttcaatgaca gagaaatggc gaaagatgcc gtgatggata
     2401 tctttgagga gcttccgcca aagctcgccc gccatgaggt tggccatttc aaggcatggc
     2461 tgtatacggt agcaaagaac cactgcctga tgcagttacg cgcttccggc cggcttaggc
     2521 tgaactccct cgatccggac cttgtgcaaa tgccggaaga aacgcatctc aacgataaat
     2581 tagaccagga ggcccggctg gaccagttgt cggattgtat ctcgaccctt tccgcagatc
     2641 aaaagacagt cgtgagcctt ttttatcttg aaaacaaatg ctataaagag atcgaatgtg
     2701 ctacggggtt cgagtggaac agagtgagga gcctgatcca gaacggccgc cggaaccttc
     2761 gcctttgcat ggaaaggcaa gaggggccgc ctacccgtgc cggtgacagc cggcccgcgc
     2821 ccgctcccga aaaaaaatga ggaagcgtca ccataccgcc cattattccg cctcagatat
     2881 ccaaaaatac ctgagagggg aaatgtctgc cgctgaaatg cacacgctgg agcgggccgc
     2941 cctcgaagat cctttcctcg ccgatgcgat tgaaggcctt gccttccatg gcggccaggt
     3001 aagcgactcc ggtcttcagc aagatctcgc ccaactgcag gaaagactga cgtcccgcac
     3061 caacgcaagg cgccggcaac atccgttctt cctaaggccg gtcagcctcg ccgccgctgt
     3121 cctgctcctt ctgggcatcg ggtacagcta tttcattttc ttcaccgcgc gtaaaccccg
     3181 gatgaccccg tcagcccacg tcgcctacac ggc
//
LOCUS       DATGKE010000317         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1460736, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000317 DATGKE010000000
VERSION     DATGKE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460736"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..489)
                     /locus_tag="VKU83_05450"
     CDS             complement(67..489)
                     /locus_tag="VKU83_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93028.1"
                     /translation="MITNLVLRLQAFLERQVKRSERFINRLPARTLWIGSAIFTAALS
                     AVLVALLVAAWKRPASYLSIKPVHVPAYVIEPRPAPAPGPPQALLQHLQHLVEQLDSL
                     HAHPNSPLYDSLIRQHPGLRDSLIEAENLFLHPYTIQP"
     gene            complement(492..1109)
                     /gene="traK"
                     /locus_tag="VKU83_05455"
     CDS             complement(492..1109)
                     /gene="traK"
                     /locus_tag="VKU83_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HLI93029.1"
                     /translation="MFSHLKRLDSAFRYVRSFTIAIVLASAGLCSFVLYKSYQLAALT
                     QNKIYILANGQALEALAADRKENIAVEAKDHIRHFHELFFNLDPDDKSIQGALTKALY
                     LVDGSGKQQFQNLKENGYYASLIAGNISQRIQIDSIELNITFYPYYFRLHAHETILRA
                     TSILHRLLVTEGYLRNVNRSDNNTHGFLIERWRTLDNSDLNTETR"
     gene            complement(1121..2158)
                     /gene="traJ"
                     /locus_tag="VKU83_05460"
     CDS             complement(1121..2158)
                     /gene="traJ"
                     /locus_tag="VKU83_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraJ"
                     /protein_id="HLI93030.1"
                     /translation="MSSRKLFFGLGALLLGPVAARAQDSWASNISSLNGVLDQIYTQM
                     LPLCSRLIGVGQGIAGFAALFYIAARVWRHLANAEPIDLFALFRPFVLGFCILIFPSV
                     IGLMNGILQPTVSATSAMVNHSDEAIAALLAEKQAAIQQTDAWQMYVGENGEGDIDKW
                     YQYTHPDDPNRADEGWLASIGTDVKFAMAKASYNFRNAIKEVIAEILQLLFAAASLCI
                     NTIRTFNLLLLAIIGPLVFGFSVFDGLQHTLKHWIARYINVFLWLPVANLFGAIIGQI
                     QQNMLKIDIAQINTTGDTFFSSTDVAYLIFLIIGIFGYTTVPGVANQIIWVSGDSLTS
                     TVTKTFRKPFS"
     gene            complement(2145..>2509)
                     /locus_tag="VKU83_05465"
     CDS             complement(2145..>2509)
                     /locus_tag="VKU83_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93031.1"
                     /translation="VARIIQNQISLVSEYKSASHQFRSSGQFNPDELAYIGSVYNNLV
                     MASLNNLTDLTTVLTAGSLRMSDAERMAAIDRIDRDVTNQLTFLRFFNNRTAVLGLQR
                     AKEQENIDELQKMYNVLP"
BASE COUNT          550 a          604 c          793 g          562 t
ORIGIN      
        1 gtagcggcag caccgtaaag aactggcgct ttttgaggaa ggcggccgaa gccggagaaa
       61 ttgtgttcat ggttgaatag tgtatgggtg aagaaataaa ttttcggcct caatgagact
      121 gtcccgaagc ccagggtgct ggcggatcag gctgtcatac agtggactgt tgggatgggc
      181 gtggagactg tcgagctgct cgaccaggtg ttggagatgt tgcaataggg cttgtggtgg
      241 tcccggcgca ggcgctggcc ggggctcaat gacataggcc ggcacatgga ccggctttat
      301 cgaaagatag ctcgcgggac gcttccaggc agccaccagc agcgcaacca atacggccga
      361 tagggcggcg gtaaagatgg cggatccgat ccacagtgtt cgggcgggca gtcggttgat
      421 aaaccgttcg gagcgtttaa cctgccgttc caggaaggcc tggaggcgga ggacaagatt
      481 ggtaatcatc attagcgtgt ttcggtgttg aggtctgaat tgtcgagcgt gcgccatcgt
      541 tcaatgagga agccatgggt gttgttatcg gaccggttga cgttgcgcaa atagccttcg
      601 gtaacgagca gccggtgaag aattgacgtt gcgcgcagga tcgtttcatg ggcatgcaac
      661 cggaaataat aaggatagaa agtgatgttg agctcgatcg agtcgatctg gatccgctgg
      721 ctgatattgc cggcaatgag gctggcataa tagccgtttt cctttaggtt ttgaaactgc
      781 tgctttccgg agccatcgac cagatagagt gccttggtca gggccccctg gatacttttg
      841 tcgtcggggt cgaggttgaa gaagagctca tgaaaatgcc ggatatgatc ttttgcttcg
      901 acggcgatat tttccttccg gtctgcggcg agtgcctcca gagcctgccc gtttgccagg
      961 atatagatct tgttttgtgt cagggcggcc agttggtaac tcttgtacag tacgaaactg
     1021 cagagcccgg cgctcgccag cacgatagcg atggtgaagc tgcgcacgta gcgaaaagca
     1081 ctgtcgaggc gtttaagatg gctgaacatg gggcagtggt ttaagaaaat ggtttgcgga
     1141 aggttttggt gacggtactt gtcaacgaat cgccgctgac ccagatgatc tggttggcta
     1201 cccccggcac cgtcgtgtat ccgaagatgc cgatgatcag aaaaatgaga taggcgacgt
     1261 ccgtcgaact gaagaaagtg tcgcctgtag tattgatctg ggcaatatcg atctttagca
     1321 tattctgctg gatctgcccg atgatcgcgc cgaaaaggtt ggcgactggc agccagagga
     1381 agacgttgat gtagcgggcg atccagtgct tcagcgtatg ctgcaggccg tcaaagaccg
     1441 agaagccgaa gaccagcggc ccgatgatgg cgagcaggag caggttgaag gtccggatgg
     1501 tattgatgca gagcgaagcg gccgcaaaca gcagctgcag gatctccgcg atcacctcct
     1561 tgatggcatt gcggaaatta tagctcgctt tggccatggc gaattttacg tctgtcccga
     1621 tactggctag ccagccttcg tcggcgcgat tggggtcatc ggggtgggtg tactggtacc
     1681 atttgtcgat atctccttcg ccgttctcgc ccacatacat ctgccaggca tccgtttgct
     1741 ggatggcggc ctgcttttcg gccaacagcg cggcgatggc ttcgtcggaa tgattgacca
     1801 tggcgctggt cgccgaaacc gtcggctgaa gaatgccgtt catgagcccg atgacagagg
     1861 gaaagatcag gatgcaaaag ccaagaacga agggccggaa caaggcgaat agatcgatgg
     1921 gttccgcgtt ggccaggtgc cgccagaccc gcgctgcaat gtaaaagagc gctgcaaaac
     1981 ctgcaatacc ctgcccgaca ccgatgagcc ggctgcacaa gggcagcatt tgggtataga
     2041 tctgatccag gacaccgttc aggctgctga tattgctcgc ccagctgtcc tgcgcccgcg
     2101 cagcgaccgg accgaggagt agggcgccga gaccaaaaaa tagtttacgg gaggacattg
     2161 tacatttttt ggagttcatc aatattctcc tgctccttgg cacgctgcag tccaagtacc
     2221 gccgtccggt tattgaagaa tcggagaaag gtgagctggt tggtcacgtc cctgtcgatc
     2281 cggtcgatgg ctgccatgcg ctcggcgtca ctcatccgga gcgatccggc cgtcaggact
     2341 gttgtcaggt ctgtgaggtt attgagcgag gccatgacca ggttgttgta gacggacccg
     2401 atatacgcca gttcgtccgg gttgaactgg ccgctcgacc gaaactggtg ggaagccgac
     2461 ttgtactcgc tgaccaggct gatctggttc tggatgatcc gggcaactt
//
LOCUS       DATGKE010000318         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1516383, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000318 DATGKE010000000
VERSION     DATGKE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516383"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VKU83_05470"
     CDS             <1..583
                     /locus_tag="VKU83_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HLI93032.1"
                     /translation="AEVADTAPLTPDIPRPVYGPYDTIIVPATVYDNELLPAKTLEYC
                     WVSAPMTAASRRRLEAWTRLKNAVYVTYPYARKAGAIINEINTKMALLRTEKERKAYL
                     QSREKELRKEFTDPLEQMSIFQGKVLMKLINRQTGNNCYEIIKEYKGGLSARFYQTVA
                     FFFSTSLKQPYDPAGEDQSIETIVQEIERMYRG"
     gene            complement(637..1122)
                     /gene="rfaE2"
                     /locus_tag="VKU83_05475"
     CDS             complement(637..1122)
                     /gene="rfaE2"
                     /locus_tag="VKU83_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HLI93033.1"
                     /translation="MPQIISQKIYDLPGMMRQVNRWRLLGKSISFTNGCFDILHQGHI
                     ASLSDAAREADMLIVGLNSDASTRRLKGRDRPVTGESARATILAALLMVDAVIVFDED
                     TPLSLIRSVRPDVLVKGGDYTLEQIVGAREVMDWGGRVVINPIVPGFSTTALIEKITR
                     L"
     gene            complement(1091..2143)
                     /locus_tag="VKU83_05480"
     CDS             complement(1091..2143)
                     /locus_tag="VKU83_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HLI93034.1"
                     /translation="MKKGLVVLLQYAFFAALAAFFVWLSLKDMDRKSWAQLRDALDRA
                     NYWLLVPVLLLLLLSHWLRALRWRQLIEPMGYEPSKMNCFLGVMIGYFVNLGAPRLGE
                     VVKCTILARYEKVPADKLVGTIVAERAFDVVCLAIVFGLTFLLQFDVIHSLITDSSLF
                     HHGKNPASPWKWRIIFGALALVILFFIVRWRWVVRRWAMIVHKVRAVVVNIVHGLTSV
                     RSIRNKPLFFFYTIGIWCLYLLSTWFGFFAISATRNLNIIDALSVLAMGSVGMIVSPG
                     GIGAYALLVRETVSFYNIPALPYGQALGWLLWFGQFLSFVLFGTASFILLPRINRKRL
                     VIVSNEIATDHIAENL"
     gene            complement(2156..2500)
                     /gene="panD"
                     /locus_tag="VKU83_05485"
     CDS             complement(2156..2500)
                     /gene="panD"
                     /locus_tag="VKU83_05485"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HLI93035.1"
                     /translation="MQIEVLKSKVHRVTITEANLNYVGSLTLDEDLMDAAGMIENEKV
                     QIVNVNNGARIETYLIKGKRGSGTCCLNGPAARKGAVGDIVIVISYAHLDFAEAKTFK
                     PWVVFPKEGNKL"
BASE COUNT          651 a          783 c          730 g          546 t
ORIGIN      
        1 agccgaagtg gcagacacgg cccctttgac gcccgacatc ccgcgtcccg tctacgggcc
       61 ttatgatacc atcatcgtcc cggcgaccgt atatgataac gaactattgc cggcgaagac
      121 cctcgaatat tgctgggttt cggcgccgat gacggccgca tcccggcgcc ggctggaagc
      181 atggaccagg ctgaagaatg cagtctatgt cacctatccg tatgcgcgca aggccggggc
      241 gatcatcaac gagatcaata caaagatggc cctgctgcgt actgaaaaag agcgaaaggc
      301 ctacctccag tcgagggaaa aggaactgcg gaaggaattt accgatcccc tggaacaaat
      361 gtcgatattc cagggaaagg tcctcatgaa gctcatcaac cgtcagaccg gcaacaattg
      421 ttacgagatc atcaaagaat ataagggcgg gctctcggcc cgtttctacc agaccgtggc
      481 tttctttttc agcacgagcc tcaagcagcc atacgatcct gcaggcgaag accagtccat
      541 cgaaacgatt gtccaggaga tcgagcggat gtatcgcggc taaggccgat ggaggtgggg
      601 cggcgacccg ttatcggctt atggcgaaac aaaagttcac aatctggtta tcttttcgat
      661 gagtgcggtt gtggaaaatc ccggcacaat cgggttgatg acgacgcgac cgccccagtc
      721 cattacctcc cgggccccga caatctgttc gagcgtgtag tccccgccct tcacgagtac
      781 atccgggcga acggaacgga tcagcgagag gggcgtatct tcatcgaaca cgatgacggc
      841 atccaccatc agcagggccg ccaggatcgt agcccgggcc gattcaccgg tgaccgggcg
      901 gtcccggcct ttcagccgcc gcgtcgacgc gtcggaattg aggccgacga tcagcatgtc
      961 cgcctcgcgg gcggcatccg acaaagaggc aatatggccc tggtgcagga tgtcgaagca
     1021 cccgttggta aaggaaatgg acttaccgag caggcgccag cgattcacct gccgcatcat
     1081 gccgggaagg tcatagattt tctgcgatat gatctgtggc aatttcatta cttacgatta
     1141 caagacgttt tctatttatt ctgggcagca ggataaagct cgccgtgcca aacaacacaa
     1201 agctaaggaa ctggccgaac cagagcagcc atcccagcgc ctgtccgtac ggcagggccg
     1261 gtatattgta aaatgagacg gtctcgcgga ccagcagcgc ataagcgcca atccctcccg
     1321 gcgaaacgat cattcctacg ctgcccattg ccagcacgct cagcgcatct ataatgttga
     1381 ggttccgggt ggcactgatg gcaaaaaacc cgaaccatgt gctcagcagg tacaagcacc
     1441 agatgccgat ggtataaaag aagaaaagcg gtttgttgcg gattgaccgg acgctcgtaa
     1501 gaccgtggac gatattgacg acgaccgccc tgaccttatg aacgatcatc gcccagcgcc
     1561 tgaccaccca ccgccaccgg acgatgaaaa agaggatcac aagggccagc gccccaaaaa
     1621 ttatccgcca cttccatggc gaagcggggt tcttgccgtg gtggaacaac gagctgtccg
     1681 tgatgaggga atggatcaca tcgaattgca acagaaaagt aagcccgaag acgatcgcca
     1741 ggcatacgac gtcgaatgcc cgctcggcta cgatcgttcc aaccagtttg tcggccggca
     1801 ctttttcgta cctcgccagg atcgtgcact tcacgacctc cccaagccgc ggtgcgccaa
     1861 ggttgacgaa ataaccgatc attaccccga ggaaacaatt catttttgaa ggctcgtacc
     1921 ccatgggctc gatcagctgc cgccagcgca gggcccgcag ccagtgactc agcaggagga
     1981 gaagcagtac cgggacgagc agccagtagt tggcgcgatc aagtgcatcc ctcagctggg
     2041 cccagctttt cctgtccata tccttcaggc tcaaccaaac aaaaaacgct gccagggcag
     2101 cgaaaaaggc atactgcaac aggaccacca atcctttctt catagccggt acaagttaca
     2161 gtttatttcc ttctttcgga aaaacgaccc atggcttaaa cgtcttcgct tctgcgaaat
     2221 cgagatgcgc gtaggaaatg acgataacga tatctcccac cgcgcctttc cttgcggctg
     2281 ggccgttcag gcagcaggtg ccggagcccc gtttcccttt tatcaggtaa gtttcgatcc
     2341 gcgcgccgtt gttcacattt acgatctgta ctttttcgtt ttcgatcatg cctgcggcat
     2401 ccatcaggtc ttcgtcaagg gtaaggctgc cgacgtaatt gagattggct tccgtgatcg
     2461 tgacccggtg aaccttcgat ttcagaactt cgatttgcat ggatggttga cctcgttttt
     2521 tagattgcgc acaaaagtaa acaaatcgcc gaaacggagc tttccctata ggagcatgtt
     2581 gtcgatgagg cgaacttctc cctggaaggc ggcgatcaat gcaacggcgg gccggctttg
     2641 cccccgcctc agcggttgga tcgtctcccg gtccgccacg gcgacgtagt ccggccggca
     2701 acccgcctcg
//
LOCUS       DATGKE010000319         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1356060, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000319 DATGKE010000000
VERSION     DATGKE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356060"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VKU83_05490"
     CDS             complement(<1..1002)
                     /locus_tag="VKU83_05490"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HLI93036.1"
                     /translation="MEKDHFVIGVDYGTDSVRSLLVNAADGTEVATAVFHYPRWKDGL
                     YCNATENQFRQHPLDYIEGLEQTILSCLSQAGRTAANSVRAISVDTTGSTPVAVDKTG
                     TPLGLLPAFRDNPNAQFVLWKDHTAVREAAEINAHAARFDTNYLRFVGGIYSSEWFWA
                     KLLHILRVDDAVRKACWSWVEHCDWIPFMLTGGRNAGEMRRSVCAAGHKALWAKEFGP
                     GGLPPDDFFSALDPLLRGFTARLFTHTFTSDQPAGTLSAEWAGRLGLPTSVVVGVGAF
                     DAHMGAVGGQIEPYHLSKVMGTSTCDMLVAPPDEIGDRLVKGICGQVPGSIIPGMTGM
                     "
     gene            1590..1805
                     /locus_tag="VKU83_05495"
     CDS             1590..1805
                     /locus_tag="VKU83_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLI93037.1"
                     /translation="MENKVKINRIKAVLAEKDMSHKELASRVGIAPNSVTRICNNEFQ
                     PTLRLLRDMAYALDVDIRELLISTRNS"
     gene            1967..2467
                     /locus_tag="VKU83_05500"
     CDS             1967..2467
                     /locus_tag="VKU83_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLI93038.1"
                     /translation="MVDLVLDNIDLRILQLMQGNARIPNVDLAKQLEMAPSAVLERVK
                     KLEQKQVILHYTTGINPAAVGQKLLAFIFIRTRDGFGSGETGARLAGIEEVQEVHNVA
                     GDDCYLVKVRTADSASLMELMRGKFAQIQSIQSTKTTIVLETVKEEQKLVIPCQAEPG
                     NPYRKK"
     gene            2473..>2669
                     /locus_tag="VKU83_05505"
     CDS             2473..>2669
                     /locus_tag="VKU83_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93039.1"
                     /translation="MSSASGQSPSAMRVAAAFTTVYLVWGSTYFFIRMSVAHIPPMLV
                     GALRYVFAGLLMLTWCVLTRE"
BASE COUNT          613 a          688 c          770 g          598 t
ORIGIN      
        1 catcccggtc atgcccggga tgatggagcc ggggacctga ccgcaaattc cttttacgag
       61 gcggtcaccg atctcatccg gcggagcgac gagcatgtcg caggtagagg tgcccattac
      121 cttgctcagg tggtaaggtt cgatctgccc gccgacggcg cccatatggg cgtcgaacgc
      181 gccgacgccg acgactacgg aagttggcag gccgagccgc ccggcccatt cggcggaaag
      241 cgtccccgcg ggctgatccg aagtaaacgt gtgagtgaaa aggcgggcgg tgaaaccccg
      301 cagaagaggg tcgagggccg agaaaaaatc gtcgggcggc aaaccgccgg ggccaaactc
      361 ctttgcccac aaagccttat ggccggcggc gcagacactt cgccgcattt cgccggcatt
      421 ccggccgccg gtaagcatga aaggtatcca gtcgcagtgc tcgacccagg accagcaagc
      481 tttcctaacg gcgtcgtcca cgcgaaggat atgcagcagc ttcgcccaga accactcaga
      541 ggaatagatt ccgccgacga agcggaggta attggtatcg aagcgggcgg cgtgcgcatt
      601 tatttcagct gcttcgcgga cggctgtatg gtctttccat aacacgaatt gagcattcgg
      661 gttgtcgcgg aacgccggca acaatccgag gggagtgccg gttttatcca ctgccaccgg
      721 ggtcgatccg gtggtgtcga cggagatagc cctgacagaa ttggcggcgg tgcgccccgc
      781 ctgggacagg caactgagaa tggtctgctc gagtccttcg atataatcca gcggatgttg
      841 ccgaaattga ttctccgtag cgttgcagta gaggccgtct ttccagcggg gataatgaaa
      901 cacagccgtg gcgacctctg tcccgtcagc agcattgaca aggagggagc ggaccgagtc
      961 agtaccgtaa tccactccga taacaaaatg atctttttcc attcggctaa ggtgctgttc
     1021 cggcgcgcgc cggtaattat tgtcaaaata acatttattt ttttggttta tgaaaaaata
     1081 atttgtcgcc cgcggcgatt gcgttgtcgc ccgcggctat ggcgggcggg cggcgcgaga
     1141 ccgttttccg cggcgggccg ggaggaccgg aacaggcagc ttagggcggg atgcccccgc
     1201 ttaacgaaga gaagatcgca aatatgctgc gcccttgctg ttccgatgcg cgacgaaacg
     1261 ttaacggcct ggtggataag acattgcgca gaacggccag gccgcgatct ttcatttacc
     1321 ccctccgccg ctcctcccgc cctgcggcgg ggccttttat ttgcagaagt atcaaaaaat
     1381 gaaggttgag cggcaaagag ggaagttttt ttcgctctgg cggggaatac gtaaaaatta
     1441 ttttctaata cctgccacag atcaaaccat gtcaataacg tatcaaataa aatggaagat
     1501 gttgattcct agaagtttat cttcgttgtt gtgtattggt tataccgtta aataccctaa
     1561 attgtcttgc tctcaactgc aggaaagaca tggaaaataa ggttaaaatc aacaggatca
     1621 aggccgtcct agcagaaaag gacatgtcgc acaaggaatt ggccagcagg gtgggtatcg
     1681 cacccaacag tgtgacaaga atctgcaata atgaattcca gcctactttg cggctgctaa
     1741 gggatatggc gtatgcgctg gatgtggaca tccgcgagtt gcttatctct acgcgcaatt
     1801 cctgattttc aagggccggc ggccaaaacg gtcgccggcg gcagcccgtc tccgggattc
     1861 caaccagctc gttttgtacc tcttttcgac cggcttgtag aattaaattc tgaagaattt
     1921 attttttttg aattttattc tatacaatat tatttttgca gaattaatgg ttgacctggt
     1981 actagacaac atcgatcttc ggatcctgca actgatgcag ggcaatgcaa ggatcccgaa
     2041 cgtagacctg gcaaagcagc tggagatggc gccttctgca gtgctcgaac gggtgaagaa
     2101 actggagcag aagcaggtta tccttcacta cacgaccggc atcaatccgg cagccgtcgg
     2161 tcaaaagttg ctcgctttca tctttatccg gacgcgggac ggtttcgggt ccggtgaaac
     2221 gggggcccgg cttgccggca ttgaggaggt ccaggaggta cacaatgtcg ccggtgacga
     2281 ttgttatctc gtgaaggtcc ggacggcaga ctcggcgtcc ctgatggagc tgatgcgagg
     2341 caaattcgcc cagatccagt cgatccagtc cacgaaaacg accattgtgc tcgagaccgt
     2401 caaagaggaa caaaagttag tgattccatg ccaggcggag cctggtaatc catatcgcaa
     2461 aaaataattc gtatgtcatc tgcttcgggt cagtctccct cggcgatgag ggtcgcggct
     2521 gccttcacaa cggtttatct tgtctggggc tctacctatt ttttcatccg gatgtcggtg
     2581 gcgcatatcc cgccgatgct ggtcggggcg ctgcgatatg tgttcgcggg cctgctgatg
     2641 ttgacctggt gtgtcctcac gagggagaa
//
LOCUS       DATGKE010000320         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_379537, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000320 DATGKE010000000
VERSION     DATGKE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379537"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VKU83_05510"
     CDS             <1..1043
                     /locus_tag="VKU83_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HLI93040.1"
                     /translation="LILLLGPALAKLLHTGPGLAMMLRWYIPGLVAMVIFSHLEATQQ
                     GHLDFKGGFAGYFVRTVVFFLLVLGEMLTGRPLTLNAVAIYLSISVVAGAIVIYGYTR
                     KYLQHRWDYERRWTKELLGFGGYIFGSGVVSNIFQNLDKVMTAALLQSNVYVAEYDAA
                     AKINQFIDTPSYAAAEVLFPKTAQASEREGPVKVRYLFERMVAALLCFTIPTAVVISV
                     FPRLVLRLIANPAYVVAAPILQLYMISGILRPAQNQAANLLNSIGKTRLSFLMNAGYL
                     TVNLGINYVCLETIGFYGAAIGSVITFLLGAVAWYFVMKRQVGFRLTGVLAYMREFGR
                     IGYGYATRLFVK"
     gene            complement(1082..1813)
                     /locus_tag="VKU83_05515"
     CDS             complement(1082..1813)
                     /locus_tag="VKU83_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLI93041.1"
                     /translation="MKLRNPKSRFDLNIGRNERVLEVGGGHNPNPRSNVVVDKFTDSN
                     YHRSGDIKVLKKQTFMEADGEALPFKDKEFDYVICCQVLEHVENPVKFLSEQFRVARR
                     GYIETPSLLGEYLFPRESHKWILHEHEDTLYLLDKKKLGFQSGYDMGELIQLYLPTHS
                     IGFKILERTHPNLFTIRIEWESDFKYVVEPGDPEIRKYFSGKWQESWGDEFFPPRSLW
                     QEFKASSAAMREISKGVFRSRFLKK"
     gene            2062..>2840
                     /locus_tag="VKU83_05520"
     CDS             2062..>2840
                     /locus_tag="VKU83_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93042.1"
                     /translation="MKNFYEVTCKKSVLLLISGGFICSGAFGQQIVTYDTTFGGWNAV
                     VTMDKALTGTDSAAGIVFFPGIGEETTDSSKIVVNGPHYLIRNGIWDGSVPLGNGVHH
                     PFIISLQPPASGYPANSVKPKIDAILARYRIKRNSFYLTGLSMGGWYANQFVCYEPTP
                     GDDSYGRLVRALVVLEGVEPSDSTGIYPSLSYPRKMGHYAKACGGRELWVEGSNDWRN
                     MLAGAQNMCDSVPNSATYFMVTYGGGAHCCWNTEYNPATKWT"
BASE COUNT          620 a          699 c          807 g          714 t
ORIGIN      
        1 gcctgatatt gctgcttggg ccggcgctcg cgaagttgct gcacaccggg ccgggattgg
       61 cgatgatgtt gcgctggtat atacccgggt tggtggccat ggtgattttt tcgcatctcg
      121 aagccacgca gcaggggcac ctggatttca agggcggctt tgccggctat tttgtccgga
      181 cggtcgtctt ctttctgctc gtgctcgggg agatgcttac cggccggccg ctgacgctca
      241 acgcggtggc gatttacctt tcgatcagcg tggttgccgg ggcgattgtc atttatggct
      301 acacgaggaa ataccttcaa catcgatggg attatgaacg gcgctggacg aaggagctac
      361 tgggcttcgg cggatatatt tttggttccg gcgtggtctc gaatatcttc cagaacctgg
      421 ataaggttat gacggccgcg ctgctgcaaa gcaatgtgta cgtcgcggag tacgatgcgg
      481 ccgcaaagat caaccagttc attgatacgc cgtcgtatgc cgcggcggag gttttatttc
      541 cgaagacggc gcaggcctcc gaacgggagg ggccggtgaa agtgcgctac ctgttcgagc
      601 ggatggtggc tgcgctgctt tgcttcacga tcccaacggc agttgtgatc agcgttttcc
      661 cgcgactggt gctccggctg atagccaacc cggcttatgt cgtggcagcg ccgatcctgc
      721 agttgtatat gattagcggg atattgcggc cggcgcagaa ccaggcggcc aacttgctca
      781 attcgatcgg caagacgagg ttgtcctttt taatgaatgc cggctatctc acggtgaacc
      841 tcggcattaa ctatgtatgt ctggagacaa tcggctttta tggcgcggcc atcggatcgg
      901 tgatcacctt cctgctgggc gccgtcgcct ggtattttgt gatgaaaaga caggttggat
      961 tccgattgac cggggttttg gcatacatgc gggagtttgg ccggatcgga tatgggtatg
     1021 ccacccgatt atttgtgaag taaggcggcg atcctgccga tttgcgggga gcagcgctgc
     1081 gctatttctt aagaaacctg gagcgaaaaa cgccctttga gatttccctc atcgcggccg
     1141 acgaagcttt gaattcctgc cagagggacc gcggagggaa aaattcatct ccccagcttt
     1201 cctgccattt gccggaaaaa tacttgcgga tctcagggtc tccgggttcg actacgtatt
     1261 tgaaatccga ttcccattcg atgcggatgg tgaacagatt tggatgggtg cgctccagga
     1321 tcttaaagcc gatggaatgt gtcggcaggt aaagctggat cagctcgccc atgtcgtagc
     1381 cggattgaaa tcctaatttt ttcttatcca ggagataaag ggtatcctca tgttcgtgga
     1441 gtatccattt gtgtgattcg cgcggaaaaa ggtattctcc gagaagcgaa ggggtttcga
     1501 tatagccgcg cctggcgacg cggaattgtt cggaaaggaa cttaaccgga ttttcaacgt
     1561 gctccagtac ctgacagcag atgacgtaat cgaactcctt atctttaaac ggaagggcct
     1621 cgccgtcagc ctccatgaat gtctgtttct tcaatacttt gatatcgccg ctgcggtggt
     1681 agttggaatc agtgaattta tccacgacaa cgttggacct ggggttcgga ttgtgccccc
     1741 cgcccacttc caacactctt tcatttcgac cgatattcag atcaaagcga cttttcggat
     1801 ttctaagctt cataacgtag ccgtaggagt tattgcatac tcaaggagcc ggaaaaatag
     1861 gatctttttc ttacttgtgc aaaagcaaat cgtaaaaatg cgatatcgcg ttgaccgtag
     1921 attatgcaaa aaaacgagta aaactgatca ctggcctttg acaaaaaatt gcattctttg
     1981 tcctactgga cccctgaccc gatccgtaca ctgattatcc aaatccgtgg agttacccgc
     2041 tcccgatgaa aaccaaatgt tatgaaaaac ttttacgagg tcacttgtaa aaaatccgtg
     2101 ctgcttttga tttccggggg gttcatttgc tctggcgcct ttgggcagca gatagttacc
     2161 tacgacacga cattcggcgg ctggaatgcc gtcgttacga tggataaagc acttaccggc
     2221 accgattcgg cggccgggat cgtatttttt cccggtatcg gggaggagac aacggacagt
     2281 tccaagatcg tcgtgaacgg tccgcactac ctgatccgaa acgggatctg ggatggcagc
     2341 gtgccgctcg gaaacggcgt tcaccatccg tttatcattt ccttacagcc accggcttcg
     2401 ggctatccgg caaactccgt caagccgaag atcgacgcga tccttgcgcg atatcggatc
     2461 aaacgaaata gtttttacct gacggggctg agtatgggag gctggtatgc caaccaattc
     2521 gtatgttatg agccgacgcc cggggatgac agttacgggc gtttggtgcg ggcgctggtc
     2581 gttctcgagg gtgtcgaacc gtcggatagt acgggaatct atccgagcct ttcttacccg
     2641 cgaaagatgg gccactatgc aaaagcctgc ggcggcaggg agctttgggt ggaagggagc
     2701 aacgactggc ggaacatgct cgccggggcg cagaacatgt gtgattctgt tcccaattcg
     2761 gctacctact ttatggtcac ttacggcggc ggagcccatt gttgctggaa taccgaatat
     2821 aaccccgcca caaagtggac
//
LOCUS       DATGKE010000321         6387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1357280, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000321 DATGKE010000000
VERSION     DATGKE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357280"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="gldN"
                     /locus_tag="VKU83_05525"
     CDS             <1..1066
                     /gene="gldN"
                     /locus_tag="VKU83_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HLI93043.1"
                     /translation="DRFENHIFITAMKKHFIRFCLLTLVVALVAGTVDAQTTHRKPKR
                     RVTHKATATQKDQSAASAAVAPKDTAKPAMVIPDADIKLDTIRRSLRNDAIIERNLVK
                     DRTPLPYMHIREDDAVYRQRVWQEIDVHEKMNLPFVYKAEEDNGSQMFINILLQAIKS
                     GQITAFDATVDDRFTTPMTFKQISENLVAKPHVIQVPDYVKDPDGSKGIMKDTLIQEE
                     FDPGNIERYEIKEEWVFDKQTSRLYVRILGIAPEKDIKNPDGSLRAVTPVFWVYYPDL
                     RPMLAKFEAYNGKNFGARMSWEELFESRMFSSRIIKSTIDNPGDLMIAQYIHDPILQL
                     LEGDKIKEKIFNYEQDLWSY"
     gene            complement(1318..3144)
                     /gene="uvrC"
                     /locus_tag="VKU83_05530"
     CDS             complement(1318..3144)
                     /gene="uvrC"
                     /locus_tag="VKU83_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HLI93044.1"
                     /translation="MTSEQFSEIAATIPVDPGIYKYFDDRDELLYVGKAKNLRKRVSS
                     YFTKTFTGYKTFELVQRIRRIEFTIVNSEQDAFLLENALIKQFQPKFNINLKDDKSYP
                     YIVIKNEPFPRVFLTRRKISDGSEYLGPYTSVGRVRELLDLVKANVQLRTCQLNLSDA
                     QVRKGKYKVCLEYHLGNCKGPCEGRQTAEDYHQGLVQLKNILKGNLGPVIQQFKKEMR
                     DLAGKMDFEQADAVRKKILHLENYESRSVIVSRRLGNADVFSIAREGDLAYINYLIIE
                     SGTIVQTHNTQVETHLEETDEEVLAFAVARLRETFNSLATELILPFAIEFPENGVEQT
                     IPKGGDKKKLLELSQKNVAYFREELRKKKILQLEGKSGDERRKVLEQLMEDLQLSELP
                     VHIECFDNSNFQGSFPVSAMVCFRDGIESKKDYRHFNVKTVQGINDFATMKEVVHRRY
                     RRLLDEGQSLPQLVIIDGGKGQLSAALESIEELGITGMMTLVGLAKNEEEIFFPGDSE
                     SVKLPFNSDSLHLIRRIRDEVHRFGISFHRLKRSKGTFVNELEQIGGIGRQTADLLLK
                     KFRSVNRIRQLSLEELAAIVGAAKAAKIYAHFREIDSPTGQK"
     gene            complement(3154..3768)
                     /locus_tag="VKU83_05535"
     CDS             complement(3154..3768)
                     /locus_tag="VKU83_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93045.1"
                     /translation="MLQFAICLAGAFFLFSACGHRAQPNPAIAADSSSRADSAGQDFF
                     PVAEVLESEIRTVDSTPMAIKEYSFSGGKTDSSFLKPVQFEALARQFLVPEFRDGSFE
                     RDFTENSFIDNATQDATFTYSTGNKDLPLQRVDIIASPKGAVHMMKSVYLERSRVSGD
                     SSILEKMYWRAGKGFEVIRLVHVKGGAPTEQREKVVWDGSGENE"
     gene            complement(3824..5488)
                     /locus_tag="VKU83_05540"
     CDS             complement(3824..5488)
                     /locus_tag="VKU83_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93046.1"
                     /translation="MKESNFHSDEFEELIREKTEQYKMYPSENVWKAVHNALHTRRKW
                     YMGTMAFLVTGILLFSGRELLFPGHSSRKLAASAASGDSFSADATRALQPQENDRSNF
                     VRVRAAGSTAAGRRASSAGTGGMTAEEPDQSYNHLSITISHPVVSQPDLSDLLSHAVQ
                     LPGAAPSLIAVAAEMADPAPKTAEADQAGLSDGWSSRNAPANAAAHHAVDGPAARNGF
                     EPLRDDAAIRGVLESLSARGRQSERAGATFRRAHQAQRGAVMADIQDAAGTAKATTEE
                     AGEQSDASRVNWLHDYAVYSLAPNPSRGRWFLQLNLAPTINFRSLSGGSMASSKQGGG
                     PMGTLRPGTGQNWVDQSPGLGVQFGGNVLYRATRNLTLKVGIQFDYSRYKMLAYTTAP
                     QQQQQPSYRTPLGNFIYMDSLFGAQPVSVNGGPMVPNPVQATLYNDYFQLSAPVGFEL
                     RVLGNERLQFNIGATIQPSYLLNTNAYVVTADYTSYTKEPSAFRRWNLTGGVEAFLSY
                     QTGSIRWQVGPEFHYQFLSTYNSGPYPISENLRGYGLKIGITKPLP"
     gene            complement(5528..6103)
                     /locus_tag="VKU83_05545"
     CDS             complement(5528..6103)
                     /locus_tag="VKU83_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI93047.1"
                     /translation="MTEEAILQGCLKNVATAQRELYNRYSPKMLAVCYRFAHNREDAE
                     DMLQEGFIKVFSQMHTFQNKGAFEGWIRRIIVHTCINNLKKNKRFNESLDIVHAHGIQ
                     IREESVPSIVQAKQIVECIRILPIGYRTVLNLYAIEGYSHKEIADMLDIEESTSRSQY
                     TRAKQMLEDILVRKKILARPKEKAEWLAAVR"
     gene            complement(6190..>6387)
                     /locus_tag="VKU83_05550"
     CDS             complement(6190..>6387)
                     /locus_tag="VKU83_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93048.1"
                     /translation="GNADYMINADGTPNTAHTMNPVPFFIIDKTWKGSIRPGKLGDIA
                     PTILTMMGLEVPPEMTGEILI"
BASE COUNT         1332 a         1757 c         1789 g         1509 t
ORIGIN      
        1 agatcgtttt gaaaaccata tatttatcac agcgatgaaa aagcatttta tcagattctg
       61 cctgctgacc cttgtggttg cgcttgtagc gggtacggtc gatgcgcaga cgacgcacag
      121 gaaaccgaag cggagggtga cgcataaggc aacggccacc caaaaggatc agagtgccgc
      181 ctccgcggca gtcgcgccca aggacacggc caaaccggca atggtcatcc cggatgcgga
      241 tatcaagctg gacacgatcc ggcgttcgct ccggaacgac gccatcatcg aacgcaatct
      301 ggtaaaagac cggactccgc tgccgtacat gcatatccgc gaggacgacg ccgtctaccg
      361 gcagcgggtt tggcaggaga ttgacgtgca cgaaaaaatg aacctgccgt tcgtctacaa
      421 ggcagaggag gataacggaa gccagatgtt tatcaatatc ctgctgcagg ccatcaagag
      481 cgggcagatc acggccttcg acgccaccgt cgacgaccgg ttcaccacgc cgatgacctt
      541 caagcagatc tctgaaaacc tcgtcgccaa accccacgtg atccaggtcc cggactatgt
      601 caaggacccc gatggttcca agggcattat gaaggacacg ctgatccagg aggagttcga
      661 tcccggcaat atcgagcggt atgagatcaa agaggagtgg gtattcgaca aacagacctc
      721 aaggctgtac gttcgcatcc tgggtatcgc ccccgaaaag gacatcaaga acccggatgg
      781 ctcccttcgg gcggtaacgc cggtcttctg ggtctactat ccggaccttc gtccgatgtt
      841 ggctaaattc gaagcctata atggcaagaa ctttggcgcc cggatgagct gggaagagct
      901 tttcgaaagc cggatgttct ccagccggat catcaaatcc acgattgata accccggcga
      961 cctgatgatc gcccagtata tccatgaccc gatcctgcag ctgctggaag gcgacaagat
     1021 caaggaaaag attttcaatt acgagcagga tctctggtcc tactaagcct cgcgcaagaa
     1081 atactgaagg ggcgccgcga cagcggcgcc ccttattttt tggcacgtaa gacaatataa
     1141 attaataagt tatagcgtga acaaaaattt aaaaataccc aaaagcgggt attggcggtt
     1201 tgatttcttt tactactttt acaacggttt ttcataggat attggatttt aaaaacgggg
     1261 ctggatgtct atccgggccc ctttttattt tccgccggta atcgcaccgt ttgcggctca
     1321 tttttgcccc gtggggctat cgatttcccg gaaatgagca tagatctttg cggccttcgc
     1381 cgcgcccacg atcgccgcca gttcttccag gcttaattgc cggatgcggt tgaccgagcg
     1441 gaacttctta agcaacaggt ctgcggtctg ccggccgatg cccccgatct gttccaattc
     1501 atttacgaag gtgcccttgc tccttttcag gcggtggaaa ctgatcccga accgatggac
     1561 ttcgtcccgg atccgccgga tcaggtgcag gctatcgcta ttgaacggca gtttcacgga
     1621 ctcgctatcc ccgggaaaga agatctcttc ctcgttcttc gccagcccca cgagggtcat
     1681 catcccggta attcccagtt cttcgatact ttcgagggcg gcgctcaact ggcctttgcc
     1741 cccgtcgata atgaccagct gcggaagcga ttgcccctcg tcaagcagcc gcctgtagcg
     1801 ccggtgcacg acttctttca tcgtggcgaa atcgttgatg ccctgcacgg tcttcacgtt
     1861 gaaatgccgg tagtctttct tgctttcgat gccgtcacgg aaacagacca tggcggagac
     1921 cggaaagctt ccctggaaat tggaattgtc gaaacactcg atatgcacgg gcagctccga
     1981 tagctggagg tcttccatta gctgttccaa tactttgcgg cgttcgtcgc ccgactttcc
     2041 ttccagctgg aggattttct ttttccgcag ctcttcgcgg aaataggcga cgttcttttg
     2101 cgagagctcg agcaatttct tcttgtcgcc gcctttcggg atggtttgct ccacgccgtt
     2161 ctcgggaaac tctattgcaa agggaaggat caattcggtg gcgaggctgt tgaaggtctc
     2221 cctgagccgg gccacggcaa atgccaggac ctcttcgtcg gtttcttcga ggtgtgtctc
     2281 cacctgggtg ttgtgggtct gaacgatggt ccctgactcg atgatcaggt aattaatata
     2341 cgccaggtct ccctcccttg cgatgctgaa aacgtcggca ttgccgaggc gccggctgac
     2401 gatcaccgac cgcgattcat aattctcgag atggaggatc tttttccgga cggcatccgc
     2461 ctgttcgaag tccatctttc cggcaaggtc gcgcatctct tttttgaatt gctggatcac
     2521 cggtccgagg tttcctttaa ggatgttttt tagctgtacc agaccctggt ggtagtcttc
     2581 cgccgtctgg cggccctcgc agggaccttt gcaattgccg aggtgatatt ccagacagac
     2641 cttgtatttc cctttccgga cctgagcgtc ggaaaggttc agctggcagg tgcgcagctg
     2701 gacattggct ttcaccaggt cgagcagttc gcggaccctg cccacggagg tatacgggcc
     2761 gaggtattcg gatccgtcgc tgatcttacg ccttgtgagg aagacgcggg gaaacggttc
     2821 gttcttaatg acaatatagg gatagctctt atcgtccttg aggttgatgt tgaattttgg
     2881 ctggaattgc ttgatcagcg cgttttccag cagaaaggca tcctgctcgg agttgacgat
     2941 cgtgaactcg atacgccgga tccgctgcac cagctcgaag gttttatagc cggtgaaggt
     3001 cttcgtgaaa taagagctca cgcgtttgcg caggttcttt gccttcccta cgtatagcag
     3061 ttcatccctg tcgtcaaaat atttgtagat tcccgggtcc accgggatcg ttgccgcgat
     3121 ctcgctaaat tgctccgaag tcatgatgca ttcttattca ttttccccgc tgccgtccca
     3181 gacgaccttt tccctttgct cagtcggggc gcctcccttt acgtgcacga ggcggatgac
     3241 ctcgaaaccc ttgccggcgc gccaatacat cttctccagg atgctgctgt cgcccgaaac
     3301 ccggctccgc tcgaggtaaa cgcttttcat catatggacg gcgcccttcg gcgacgctat
     3361 gatatccacg cgctggagcg gcaggtcttt gttgcctgtg gaataggtaa aggtggcgtc
     3421 ctgggtggca ttgtctataa aggaattttc cgtaaaatcc cgctcaaagc ttccgtcgcg
     3481 gaattccggc acaaggaatt gcctggccag cgcctcgaac tggaccggct tcagaaagga
     3541 agagtcggtc ttgcctccgc tgaagctgta ttcttttatc gccatcggcg tcgagtccac
     3601 tgtccggatc tccgattcga ggacttctgc cactgggaaa aagtcctggc cggcggagtc
     3661 tgcacgtgat gacgagtcag cggcaatggc ggggttcggc tgggcacgat gcccacaggc
     3721 gctgaataaa aagaacgcgc cggcgaggca gattgcgaat tgtagcatgg ggcaaaatta
     3781 cgaaaaacca cccggtccgc gcgaaaggcg gacagaagcg aggtcaggga agcggcttgg
     3841 taattccgat cttcaggccg tagccgcgga ggttctcgct gatcggatag gggccactgt
     3901 tataagtgga aagaaactgg tagtggaact cggggccgac ctgccagcgg atggacccgg
     3961 tttgatagga gagaaaggct tccacgccgc cggtaaggtt ccatctgcgg aacgccgagg
     4021 gttccttggt atagctggta taatccgcgg tcacgacgta tgcattcgta ttcagcagat
     4081 agctgggctg aatggtggcg ccaatattaa attgaagccg ctcattgccc aggacgcgga
     4141 gctcgaagcc aacgggcgcc gagagctgga aataatcgtt gtaaagcgtt gcctgtactg
     4201 gatttggtac catcgggccg ccgtttaccg ataccggttg cgccccgaag agggagtcca
     4261 tataaataaa attcccgagc ggcgtccggt agctgggctg ttgctgctgc tgcggcgccg
     4321 tcgtataggc aagcattttg tagcggctgt agtcgaactg aattcccacc ttcaatgtca
     4381 ggttgcgggt cgcgcggtac agcacgttgc cgccgaattg aacgccgagg ccgggtgact
     4441 gatcgaccca gttctgtccg gtccccggac gcagggtgcc catcggcccg ccgccttgct
     4501 tggagctggc catcgagcca ccggacaggc tgcggaaatt aatggtcggc gcaagattca
     4561 gctggagaaa ccaccggccg cggctagggt tcggcgcaag cgagtatacg gcatagtcat
     4621 gaagccaatt gaccctcgat gcatcggatt gctcaccggc ctcttcagtg gtggcttttg
     4681 cggtccccgc cgcatcctgg atatcagcca tcaccgcacc gcgttgcgcc tggtgcgccc
     4741 gccggaaggt tgcgccggcc ctttctgatt gccggccgcg cgcgctcagg ctttccagca
     4801 cgccgcggat cgccgcatca tcccgaagcg gctcgaagcc gttgcgggca gccggcccat
     4861 cgacggcatg atgcgcagcg gcatttgccg gtgcattgcg cgaggaccag ccatcggaca
     4921 atccggcctg gtcagcctcc gctgtttttg gcgcaggatc ggccatctcg gctgccaccg
     4981 caatcaacga cggagccgcg cctggcagct gtacggcatg gctcagcagg tccgaaaggt
     5041 cgggttggct gacaacagga tggctgatgg taatcgagag gtggttatag gattggtccg
     5101 gttcctccgc cgtcattccg cccgtaccgg cagaactggc gcggcggccg gcagcagtgc
     5161 tcccggcggc ccggacccgg acaaaattgc tgcggtcatt ttcctggggc tgcagggcgc
     5221 gggtggcgtc tgcgctgaag ctatcgccgg aagccgcact ggcagcgagt ttccggctgc
     5281 tgtgtcccgg gaacaacaac tcccgtcccg aaaaaagcag aataccggtt acaagaaagg
     5341 ccatcgtacc catgtaccac ttgcggcgcg tatgcaaagc attgtggaca gctttccaaa
     5401 cgttttcgga agggtacatt ttatattgct ctgtcttttc cctgatcagt tcttcgaatt
     5461 catcactatg gaagttgctt tccttcatcg tcaatcagtt tttgggacgc tttcatggat
     5521 cgggtgatta gcggaccgcc gcaagccatt cggccttttc cttcggcctg gcgaggatct
     5581 tcttcctgac gaggatatcc tccagcattt gcttggcccg ggtatattgc gaccggctgg
     5641 tgctttcctc tatgtcgagc atgtcggcga tttccttatg ggaatagcct tctatcgcat
     5701 ataggttcag gacagtccga tagccgatcg gcaggatgcg gatgcattcc acgatctgtt
     5761 tggcctgcac gatcgatggc acgctttcct ccctgatctg gatcccgtgc gcatgcacga
     5821 tgtccaggct ttcattgaac cgtttgttct tcttcaggtt gttgatacag gtatgaacga
     5881 tgatccgcct gatccaacct tcgaatgcac ccttgttttg aaaagtgtgc atttgggaaa
     5941 acactttaat gaaaccttcc tgtaacatat cctccgcgtc ttccctgttg tgggcgaacc
     6001 ggtagcagac ggccagcatc ttggggctgt agcgattgta cagctcgcgt tgggcagtgg
     6061 cgacgttctt caaacagcct tgtaaaatgg cttcctcggt catactaacc ggattggttg
     6121 ccctgtaatt tagacaattt tgacgaggta aagctgcgcg ccgaaaaaaa aagatcgatg
     6181 aaacccttgt cagataagaa tttccccagt catctcgggc gggacctcta atcccatcat
     6241 tgtcaaaatg gtaggcgcaa tgtcgccgag cttgcccggg cggatgctac ccttccatgt
     6301 tttatcgatg ataaagaagg ggacggggtt catcgtatgc gccgtgttcg gcgtgccatc
     6361 cgcattgatc atgtaatcgg cgttccc
//
LOCUS       DATGKE010000322         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1195143, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000322 DATGKE010000000
VERSION     DATGKE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195143"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..1190
                     /locus_tag="VKU83_05555"
     CDS             489..1190
                     /locus_tag="VKU83_05555"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HLI93049.1"
                     /translation="MTCRFARDFRRYPGLPIVLGLVCALWSCPAGAQTGGPLHSPLLP
                     GKLVWENTPLRYELATDHILIESGGKTDMFRDPNVTYNTDNAPKLLFVPDSNFVFTAA
                     IHHDFVSKWDGGAIVLKADSLNWIKFCFEKDYTGQRRVVSVVTKGISDDCNSAAINGS
                     TVFYKIAKAGNVITLYYSLDGRGWFLVRHLTFDSTAPLKLGFLAQSPTGERCTVRFSH
                     IHYSPRKIRDPYLGE"
     gene            complement(1545..>2756)
                     /locus_tag="VKU83_05560"
     CDS             complement(1545..>2756)
                     /locus_tag="VKU83_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93050.1"
                     /translation="YRYARRTHNDSLLQKALLTKELALSFPQRNGLFPAVIGTEMITK
                     DIAGRTVNLSAGWDHYFFGNSNRNPESPGGAAQEAPYHILDMSWTADQMLGWYSELEK
                     DPRLLAYAEQYATRLVQLQDSAGYFPAWIDVKTGQVLPQLRQSPETSASAGFLLSLYG
                     ITRDERWRSAALRALAAVVRDILPSGRWEDFETYWSCSRIYVDSLGRKLHRNDQYKQN
                     TLSMYWTADACLRAYLETRDSSWLKAGRRTLDELLMYQASWQPPFIYVHALGGFGVMN
                     ADAEWNDARQSLFAELIIQYGRLLNEEEYVERGIAALKASFVMMYCPENPETETQWQK
                     VYPFFTAKDYGFMMENYGHGGVTSPDGLGIGEFTIYDWGNGAAAEAYNRILDHFSDLL
                     QTAPKPFSPTR"
BASE COUNT          596 a          800 c          741 g          619 t
ORIGIN      
        1 aaagtttggc ggcggggcgc acaaagcgcc ctatctctac cggttcaaac cggccccagg
       61 cccggaagcc ggcgcggaca tcggaccgga ccccggcgcg gaagccggcg cggacccccg
      121 accgaaagcc ggcgcataaa aaaaccgccc cggaaggagc ggtaaaaaat ctttgtcagg
      181 gttctctaat caagccttca caaggatttg gatcggattt ttaggatctt ggatcttctc
      241 tgtcaggttt tcagggattc gggatctggt taatggattc ggatcggtgg ttggctttca
      301 agggattttg gatcgggctt ttcacttctt ggactaaggg tctttctgga ctggatttct
      361 ttcgttgctg gaacaaagat gcaaaacggc atccctcacg tgaaattttt tcgccgaaag
      421 accggttctg cccgacgaaa gccggcttta cccgacgaaa tactagtata ttcccagaaa
      481 attgccccat gacctgccga ttcgcccgcg attttcgccg ttatcccggc ttgccgatcg
      541 tccttggcct ggtttgcgcc ctctggtcct gccccgccgg cgcgcaaacc ggtggcccgc
      601 ttcattcccc cttattgccc ggaaagcttg tctgggaaaa caccccgctc cggtatgagc
      661 tggcaacaga ccatatcctg atcgaaagcg gcgggaagac cgatatgttc cgggacccga
      721 acgtgaccta caacactgat aacgcgccca agctgctgtt cgtccccgac agcaatttcg
      781 tctttacggc agccatccat catgatttcg tcagcaagtg ggacggcgga gcgatagtcc
      841 ttaaagcgga cagcctcaac tggataaaat tttgctttga aaaggactat accggccagc
      901 ggcgcgtggt cagcgtcgtg acgaagggaa tttccgatga ctgcaactcg gccgccatca
      961 atggcagtac cgtcttttac aagattgcca aggccggtaa tgtcattacg ctgtattact
     1021 cgttggacgg gcgcggctgg ttcctcgtcc gccacctgac cttcgacagc acggcgccgt
     1081 tgaaactggg attcctggcc cagtcgccca caggcgagcg ctgtacggtc cgcttctccc
     1141 atatccatta cagtccgcga aagatcaggg acccgtacct gggggaatga caagacggat
     1201 tactaactta tttagcatac ctgcgcaaaa aaaaccgccc tgcacctggg cggtaaaaat
     1261 tctttttttt cagggttcaa ccaatagaaa cggtttttaa ggggttttgg attagtttat
     1321 tggttttcaa ggaaatttgg atggataagt tggactttga ctggtttcgc tttcggatct
     1381 ggatctcttt cgttgctgat acaaagatgc ccatttacag tgccggaagc aaatgttttc
     1441 ggccaacgac cggttccgcc cgacgaaagg cgaaaatgcc cgaccaacca ccggtcagcc
     1501 gcccgactgc cggacggcgg ctcggctaag gcctcgccaa ccccttaccg cgtcggcgaa
     1561 aaaggctttg gcgccgtctg cagcaggtcg ctaaaatggt ccagaatgcg gttgtatgct
     1621 tcggcggcgg cgccgttacc ccagtcgtaa atggtgaatt ctccaatgcc cagcccatcc
     1681 ggactggtaa ccccgccgtg gccgtagttt tccatcatga atccatagtc cttcgccgtg
     1741 aagaaaggat agaccttctg ccattgggtt tccgtctccg gattctccgg gcaatacatc
     1801 atgacaaatg aggcttttag cgctgcaatc ccccgctcga catattcctc ttcgttcagc
     1861 aaccggccgt actggatgat cagctcggcg aagaggcttt gccgggcgtc attccattcc
     1921 gcatccgcat tcatcacgcc gaagccgccc agcgcatgca catagataaa tggcggctgc
     1981 cagctcgcct ggtacatcaa caattcatcc aatgtccggc gccccgcctt cagccaggag
     2041 ctgtcccgcg tctccaggta cgcgcgaagg caggcatcgg cagtccaata catggagagg
     2101 gtattctgct tgtactgatc attgcgatga agcttgcgtc ccagggaatc aacatagatc
     2161 cggctgcagg accagtacgt ctcgaaatcc tcccaacggc ccgagggcag aatatccctg
     2221 accactgcgg ccagggccct gagggcagcc gatctccagc gttcgtcgcg cgtgatgcca
     2281 tagaggctca atagaaagcc ggcagacgcc gaagtctcgg gggactggcg cagctgcggc
     2341 agcacctgac ctgtcttcac gtcgatccat gccggaaaat aaccggctga atcctgcagt
     2401 tgcacgaggc gcgtcgcata ttgttccgcg tatgccagca gtcgcggatc tttttccagt
     2461 tcgctatacc acccgagcat ctggtctgcc gtccagctca tgtcgagaat atggtacggc
     2521 gcctcctggg cggcgccgcc gggagattca gggttccggt tggaattgcc gaaaaaataa
     2581 tgatcccagc ccgcgctgag attgaccgtc ctgcccgcaa tgtccttcgt tatcatttcc
     2641 gtaccgatga ctgcggggaa caagccgttc cgctggggaa acgacaatgc cagttccttt
     2701 gtcagcaagg ccttttgcaa cagggaatca ttgtgcgtcc ggcgggcata acggta
//
LOCUS       DATGKE010000323         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_951180, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000323 DATGKE010000000
VERSION     DATGKE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951180"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VKU83_05565"
     CDS             <1..552
                     /locus_tag="VKU83_05565"
                     /inference="COORDINATES: protein motif:HMM:NF036547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI93051.1"
                     /translation="ELKNIAEQISVLSEDKHISGDTLRRFIPESTVNRLPMVVTSNGS
                     MSNGNHEFNNEREILYTLFFDMKKDVTELKKMFREIVQNPALASAQHSSFLNDLKELK
                     PAEIYSAPALGAGQPVVIHNGHDDIHQHEEVEESLNIMDKEKELIVKALKKHKGKRKD
                     AASDLGISERTLYRKLKEYDIDE"
     gene            545..1069
                     /gene="lptE"
                     /locus_tag="VKU83_05570"
     CDS             545..1069
                     /gene="lptE"
                     /locus_tag="VKU83_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HLI93052.1"
                     /translation="MNKITGALSLILLLGLASCKIYSFKDTSIPAEVKTIHLAYIENR
                     ARLVNPALAPALNDALRQKINNQASRLSQIQTNDADYDITAWVSDYNYSTSGVSGQQA
                     SSNRLSVTVHIVFKNNLDPTGKKVAPANFETDVTRNFDFSAQTTITDAEAQLLPTITS
                     NLTDEIFNKLFSNW"
     gene            1093..2133
                     /locus_tag="VKU83_05575"
     CDS             1093..2133
                     /locus_tag="VKU83_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93053.1"
                     /translation="MQPAIHHLFQVSSIDGVTREQLEGMIEEYPCFGYGHFLLSRKLK
                     ATNSDQFLASAQRASLYFPNPFWLQWMLDYEPDATPPRTMPGRVTTGSPASPEPVETT
                     GSAGQIIDESAAELLMDSMEKAKELRESLQKINEDFVARTGHPIPVRETGPEPAFQPE
                     PVREEWLEPAPEPAPESVPVTQDQPIRDEELPFVLDEREQASGAIPPVAEEPVRPAPS
                     SPGETDTLFEPLHTIDYFASQGIRLTLEENPTDQLGRQLKSFTEWLRVMRRLPQREKE
                     IIPDRVAEQAVQDFAAHSIEGKEPVTETMAEVLLKQGMPERARLVYEKLSLLNPDKSA
                     YFAAKIEQLNSH"
     gene            2135..2470
                     /gene="secG"
                     /locus_tag="VKU83_05580"
     CDS             2135..2470
                     /gene="secG"
                     /locus_tag="VKU83_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLI93054.1"
                     /translation="MLFFVFIIIVVCVILGGIILIQNPKGGGLAGNVGGFSNQLMGVK
                     QTTDVLEKGTWVMAIVVGLLCLFSAVFIPKGASSNARVPEKVNAQSVPQQQSPLAPVN
                     AGRQPAGKP"
     gene            2578..3618
                     /gene="mltG"
                     /locus_tag="VKU83_05585"
     CDS             2578..3618
                     /gene="mltG"
                     /locus_tag="VKU83_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLI93055.1"
                     /translation="MKRFLVTILVIVVLALGFVAWRIFGPGTAFSGDSYALYIRTGMT
                     YPQLRNLLEKDTVIKSPAFFDWIAQRMNYPANLKAGKYDIKKDMSLISILRVLHNGRQ
                     TPVHLVILKFRTAEGLAGAVGKKFECDSDGIAAFIHNNDSLRPFGVDTNTFLTIVMPN
                     TYTYFWNTPPSAIFRKMYAGYRTWWTPRRINEARSKGLNPTTATILASIVEEETTIEP
                     DKGKIASVYLNRLARGIRLGADPTVKYALRDFDMKRVYDKDLKVASPYNTYEHNGLPP
                     GPICTPSPQTIDAVLTAPATDYLFFVARPDNSHYSNFASTYKQHLENAKAYQQWLDRQ
                     MAIRAHNDSLKK"
     gene            3625..4566
                     /locus_tag="VKU83_05590"
     CDS             3625..4566
                     /locus_tag="VKU83_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLI93056.1"
                     /translation="MIKLERNIYQSKPFRFLEKRSRKWSIPGIQGVSLYEIISFFIGQ
                     VKKVGLIDRARSIAFSFLIAIPAAAIFICTLIPYMPVSRQIMRQLLLLTRDLTPNQNT
                     YMLVSKFLEDFFSKPRVGLLSFGFLLALFYSSNAMMGIMRSFNKSLVYNARRNPIATR
                     WMAIKLTALVLCMVIGSVIIMVTQAELLRDILRDLHISSRNARWLFRTVRWIIIIPLF
                     YVAIACIYKYGPAVQKRWKLFSPGTFLATLLTILTTVFFSYWVNRFGNYNKIYGSIGT
                     VMILMLLVYFNSLVLLIGYELNVSIYHLKLMAGDRSN"
     gene            4629..>5015
                     /locus_tag="VKU83_05595"
     CDS             4629..>5015
                     /locus_tag="VKU83_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLI93057.1"
                     /translation="MKPLLLALVLLFPFGPFPGLPLGSPVPKANVPIRDVSGKQISLK
                     QASGANGLLVMFSCNTCPYVMRNQARTKEVCAYAGSHQIGVVLLNSNANSRDNGNSFA
                     DMQSYARAQDYKWYYAIDDDGSIAAAF"
BASE COUNT         1199 a         1457 c         1246 g         1113 t
ORIGIN      
        1 gaattgaaaa atattgcgga acagatctcc gttctttcgg aagacaagca catcagcggc
       61 gacacgctga ggcgcttcat ccccgaatct accgtgaacc gcctgcccat ggtcgtgacg
      121 tccaatggat cgatgtccaa cgggaaccat gagttcaaca acgagcgcga gatcctatac
      181 acactctttt tcgatatgaa gaaggatgtg accgagctga aaaaaatgtt tcgcgaaatc
      241 gtccagaatc ccgccctggc ctcggcccag cattcctcat ttctgaatga cctgaaggag
      301 ctgaagcccg ccgaaatata ctccgctccg gcgctgggag ccgggcaacc ggtggtaata
      361 cataacggtc atgacgatat tcaccaacac gaagaggtgg aggaatcgct caacatcatg
      421 gataaggaaa aggagctgat tgtgaaggcg cttaagaaac acaagggcaa acgaaaggac
      481 gcggcatccg atctgggtat cagcgaacgg accctgtacc gtaaattaaa agagtacgat
      541 atcgatgaat aaaataaccg gcgctctttc gctcattttg ctgttgggac tggcttcctg
      601 caagatctat tcgttcaagg acacatccat tccggcggaa gtgaaaacca tccatcttgc
      661 ttacattgag aaccgggcca ggctggttaa tcccgcgctc gctccggcat tgaacgacgc
      721 cttgcgccaa aagatcaaca atcaggcatc gcgcctttcc cagatccaga cgaatgacgc
      781 cgattatgat attactgcgt gggtctccga ctacaattac agcacatcgg gagtttcggg
      841 ccagcaggcc tcgagtaacc gcttgagcgt gaccgttcat atcgttttta agaataacct
      901 cgatccgacc gggaagaaag tagcgcccgc aaatttcgaa accgacgtga cgcgaaattt
      961 cgatttttcg gctcaaacca ccattacgga cgctgaggca caattattgc ccacgatcac
     1021 cagcaatctg accgacgaga tttttaacaa gttattttcg aactggtaga aaggagaatc
     1081 atgaatcaat ccatgcaacc tgctatccac caccttttcc aggtctccag catcgacggt
     1141 gtcacccgcg agcaactgga gggaatgatc gaagaatatc cctgcttcgg ctatggccac
     1201 ttcctgctct cccgaaagct gaaggccacc aacagcgacc agttccttgc gtccgcccag
     1261 cgggcaagcc tgtatttccc gaatcctttc tggttgcagt ggatgctcga ttacgagccc
     1321 gacgcgacgc cgccccggac aatgcctggc cgggtcacca ccgggtcgcc ggcctcaccg
     1381 gaaccggttg agacgaccgg gtctgcgggt cagataattg acgagtctgc ggcggagctc
     1441 ctgatggaca gcatggaaaa ggcaaaagag cttcgcgagt ccctgcaaaa gatcaatgaa
     1501 gatttcgtgg cgcggaccgg ccatccgatc cccgttcgtg aaaccgggcc ggagcccgcc
     1561 ttccagccgg aacctgtccg cgaggaatgg ttagagcccg cgcccgaacc ggcccccgag
     1621 tcggtgccgg tcacccagga ccagcctatc cgcgacgagg agcttccctt tgtcctcgat
     1681 gagcgggaac aagcttccgg ggccataccg ccggtggccg aagaacccgt ccggcccgcg
     1741 ccatcttcac cgggcgaaac cgacacccta ttcgagccct tgcacaccat cgattatttc
     1801 gcatcccagg gcatccgcct gacgctggag gaaaatccga ccgaccagtt ggggcgccag
     1861 ttgaaaagct tcaccgaatg gctccgggtc atgcggcgcc tgccgcagcg ggaaaaagag
     1921 atcattcccg accgggtcgc tgaacaagcc gtacaggatt tcgccgccca ttccatagaa
     1981 gggaaagaac ccgttacgga aacgatggcg gaggtgctcc tgaagcaagg gatgcctgaa
     2041 cgcgcgcggc tcgtatacga gaaattaagt ttgctcaatc ccgataaaag cgcgtatttt
     2101 gcagccaaaa tcgaacaact aaattctcat tgacatgctc tttttcgtgt tcattatcat
     2161 cgtggtgtgc gtgatcctcg gaggcatcat cctgatccag aatcccaaag gcggcgggct
     2221 tgccggcaac gtcggcggtt tcagcaacca gctgatgggc gtcaagcaga ccacggatgt
     2281 actggaaaaa ggtacctggg taatggcaat cgtcgtgggg ctcctttgcc tgttctccgc
     2341 ggtttttatc ccgaaggggg cctcttcgaa tgcgcgggtt ccggagaaag tgaacgctca
     2401 gtccgtacct caacaacagt cgccgctcgc cccggtaaac gcaggcaggc agcctgcggg
     2461 caaaccataa gcaaattata agcgaaaaga ccgcaaaaca atattttcag ccatcccggt
     2521 gtttagtttc cagagatggc tgattttttt tattttaggt ggacaatcga atttcctatg
     2581 aagcgattcc tcgtcaccat cctcgtgatc gtcgttctag cgcttggttt cgtggcctgg
     2641 cgcatttttg gccccggtac ggctttcagc ggggacagct acgcgctcta cattcgcacc
     2701 ggcatgacct atccgcagct gcggaatttg ctggagaagg ataccgtcat caaaagccca
     2761 gccttctttg actggatagc ccaaagaatg aattatcccg ccaacctgaa agcgggcaag
     2821 tacgacatca aaaaggacat gagcctgatc agcatcctgc gtgtccttca taatggccgc
     2881 cagactccgg tccacctcgt catcctgaaa tttcggactg ctgaagggct cgccggcgcg
     2941 gtcgggaaga aattcgaatg cgattccgac ggcatcgcgg ccttcatcca taacaatgac
     3001 tcgctgcggc ccttcggagt cgataccaac acttttctga cgatcgtcat gcccaatacg
     3061 tacacctatt tctggaacac gccgccctcc gcgatcttca gaaagatgta tgccggctat
     3121 cgaacgtggt ggacgccgcg ccgcatcaac gaggccaggt ccaaggggct taatccgacg
     3181 acggctacca tcctcgcttc tatagttgaa gaggagacca cgatcgaacc ggataaagga
     3241 aagatcgcca gcgtttatct taaccggctc gccagaggta tcaggctggg agcagatcca
     3301 accgtgaaat atgcgcttcg cgacttcgac atgaagcgcg tttacgacaa ggacctgaaa
     3361 gtcgcatccc cctataatac ttatgaacac aacggcttgc caccggggcc catttgcacg
     3421 ccttcccccc agacgatcga cgccgtgctg acggcgcccg cgacggacta cctgtttttc
     3481 gtggcccgcc ccgacaacag ccattattcc aattttgcga gcacctacaa gcaacacctt
     3541 gaaaatgcga aagcctacca acaatggctg gaccgccaaa tggccatccg ggcacacaat
     3601 gacagcctga agaaataatt cctaatgata aagctcgaac gcaatatcta tcaaagcaaa
     3661 cccttccggt tcctggaaaa aaggagccgg aaatggtcca ttcccggaat tcagggcgtt
     3721 tcgctttatg agatcatcag cttttttatc ggccaggtaa agaaggtcgg gctgatcgac
     3781 agggccagat ccattgcttt tagcttcctc atcgccattc cggccgccgc catttttatt
     3841 tgtacactga tcccttatat gccggtatca cgacagatca tgcggcaact gctcttattg
     3901 acccgcgatc ttacgcccaa ccagaacacc tacatgcttg tcagcaagtt cctggaagac
     3961 tttttttcca agccccgggt cggccttttg tcattcggat ttctgctggc gcttttctac
     4021 tcttccaatg caatgatggg catcatgcgt tcgtttaaca aatccctggt gtacaacgcc
     4081 cggcggaacc cgatcgccac gaggtggatg gcgatcaaac taacggccct tgtactttgc
     4141 atggtcatcg gctcggtaat catcatggtt acccaggccg aattactccg cgatatcctc
     4201 cgcgatctcc atatcagcag tcgcaatgcc cgctggcttt tccgcacggt ccgctggatc
     4261 atcatcatcc cgcttttcta cgtcgctatc gcctgtattt ataagtatgg tcccgcggta
     4321 caaaaacgtt ggaaactgtt ttcccccggc acctttctcg ctaccctgct gactatcctg
     4381 accacggttt tcttctcata ctgggtgaac cgctttggca actacaacaa gatttacggt
     4441 tcgataggca ccgtaatgat cctcatgctc ctcgtctact ttaattcact cgtgttattg
     4501 atcggatatg agttgaatgt cagcatctat cacctcaagc tcatggccgg cgaccgcagc
     4561 aactagtcgc ctcccctcag gtttttcgcg ccttttttcg taaactttgt gtataaaaac
     4621 cttgggtgat gaaaccgctt ctcctcgcgc tcgtcctgtt gtttccgttc gggcccttcc
     4681 ccggccttcc gcttggatcg cccgtgccga aggccaatgt cccgatcagg gacgtctccg
     4741 gtaaacagat cagtctaaag caagcgagcg gggcgaatgg cctgctggtc atgttttcct
     4801 gcaatacatg cccttacgta atgcgtaacc aggcgcgcac aaaagaggtc tgcgcctacg
     4861 ccggcagcca ccagatcggc gttgtgcttc tgaactccaa tgcgaattcc agagataacg
     4921 gcaattcctt cgccgacatg caatcttatg cccgggcgca ggactacaaa tggtattatg
     4981 ccatcgatga cgacggctcg atcgcggcgg ccttc
//
LOCUS       DATGKE010000324         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1294103, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000324 DATGKE010000000
VERSION     DATGKE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294103"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VKU83_05600"
     CDS             <1..1704
                     /locus_tag="VKU83_05600"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI93058.1"
                     /translation="INYTNNSPDKLDFLWLQLDQNLFRPDSRGAATTPISGDRFDVKG
                     FSKGGYHIESVEVTYDEKAYSVEPIITDARMQVRLQSPVKPGGDKIKVKVKYSFAIPI
                     YGADRMGRLYTKNGVVYELAQWYPRMCVYDDIESWNTLPYMGLGEFYCEYGNFDYYLT
                     TPADMTVVASGDLQNPQEVLTAREVGRLAKARKSDETVMIVTPDEAGQASSRPKMTGT
                     LTWHFKMDNTRDVSWAASKAFIWDAARVDLPSGRTAIAQSAYPIESAGKDRYGRSTEY
                     LKRSIEIYSKLYFEYPWNSAVSVAGVALGMEYPGIIFCKSDLTDGGLWGDVTHEIGHN
                     WFPMIVGSNERRFMWQDEGFNTFINEFSTRDFNRGEYYRKIPPEFIFRSFSRDRDPLM
                     TAPEGMSLRGYGAYYSKTAAGLDLLRDVIVDSNRFDYAFRQYIQRWAFKHPQPDDFFR
                     TINSVTGENLDWFWKEWFFTNDRLDQAVTSVKYVDGDSTKGALITIDNLDRMAMPVIA
                     RITDASGSSQIVKLPVDIWQRGGTWTFKYPSTTGLKSVELDPDHVLPDVNRQNNVWKG
                     E"
     gene            1770..2240
                     /locus_tag="VKU83_05605"
     CDS             1770..2240
                     /locus_tag="VKU83_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLI93059.1"
                     /translation="MLSLLSVLMYAALTITSPAFGANSMIPAKYTCQGQSISPALHLG
                     EFPSQTKSLAIIVHDPDAGHDGFTHWVAWNIHPMNDIPENYKGGVQGLNGAHKAGYMG
                     PCPPSGVHHYHFMVYALDTRLDLGTRSDKADLEKAMQGHIVAQGDLVGLYSKGK"
BASE COUNT          529 a          596 c          654 g          484 t
ORIGIN      
        1 atcaactata ccaacaatag tccggataaa ctggatttcc tctggctgca gctcgaccag
       61 aacctcttca ggccggacag ccggggcgct gcgacgactc ccatctctgg cgaccgtttc
      121 gacgtcaaag gtttttcgaa aggaggctat catattgaat cggtcgaggt cacctatgac
      181 gagaaagcct attccgttga gcccattatc accgatgccc ggatgcaggt ccggttacag
      241 tcgccggtga agcccggtgg cgacaagatc aaagtcaagg tgaaatacag cttcgcgatc
      301 ccgatctatg gggccgaccg catggggagg ctatacacta aaaatggcgt ggtatatgaa
      361 ctcgcgcaat ggtacccgcg catgtgcgtc tatgacgaca tcgaatcatg gaacacgctt
      421 ccttatatgg ggctcggtga gttctactgt gaatacggca actttgatta ctatctgacg
      481 accccggcag acatgaccgt agttgcttcc ggcgacttgc aaaacccgca ggaagtgttg
      541 accgcccgcg aggtggggcg cctggcaaag gccagaaaga gcgacgagac cgtgatgatc
      601 gtcacgcccg atgaagcagg gcaggcttcg agccggccca aaatgaccgg cacgcttacc
      661 tggcacttta aaatggacaa tacgcgggat gtgtcatggg cggcatcaaa agcattcatt
      721 tgggacgcag ccagggtcga cctgccttcc ggccggacgg caatcgccca atcggcctat
      781 ccgatcgaat ctgccggtaa ggaccgctat ggtcgctcca cggaatacct gaaacgcagt
      841 atcgagatct actccaagct gtactttgaa tatccctgga attcggccgt cagtgtcgcg
      901 ggggtagcgc tggggatgga atatcccggc atcatctttt gcaaatcgga cctgacggat
      961 ggcggccttt ggggagacgt tacgcacgag atcggccata actggtttcc catgatcgtg
     1021 ggttccaatg agcgccggtt catgtggcag gatgaagggt ttaatacctt tatcaacgaa
     1081 ttctcgactc gggacttcaa ccgcggcgaa tactaccgga agatcccgcc ggaattcata
     1141 ttcaggtctt tttcgcgcga cagggatccg ctgatgaccg cgcccgaggg gatgagcctg
     1201 agaggatatg gcgcctacta cagcaagacg gctgccggcc tggacctgct gcgggacgtt
     1261 attgtggaca gcaaccgttt cgattatgcc ttccgccagt acatccagcg ctgggcgttc
     1321 aagcacccgc agccggacga tttcttccgg accatcaaca gcgttacggg cgaaaacctc
     1381 gactggttct ggaaagaatg gttctttacc aatgaccggc ttgaccaggc tgtcacctcg
     1441 gtcaaatatg tggacggcga ctcgacaaag ggcgcgttga tcaccatcga caacctggac
     1501 agaatggcaa tgccggttat tgccaggatc accgatgcat cgggcagttc ccaaatcgtc
     1561 aagctgcccg tggacatctg gcaacgcggt ggaacctgga cgttcaagta tccttcgact
     1621 acgggattga aatccgtgga gttggatcca gaccatgtac tgccggatgt gaaccggcag
     1681 aataatgtgt ggaaaggaga gtaaggggtt ccgcgtgtct gcctgatccg ctggtacacc
     1741 gtttgcgagt aggctttaaa agaccctgta tgttatcgct attaagcgtt cttatgtatg
     1801 cggctttaac tataaccagt ccggcgttcg gcgcgaattc catgatcccg gccaaatata
     1861 cgtgtcaggg ccaaagcatc agcccggcgc tccatcttgg ggagtttcct tctcaaacca
     1921 agtcgctggc catcatcgtg catgacccgg atgccggcca tgatggtttt acgcattggg
     1981 tcgcctggaa catccacccg atgaacgaca ttcccgagaa ttacaagggc ggggttcagg
     2041 gattgaacgg cgcgcacaag gccggctaca tggggccttg cccgccaagc ggggtccatc
     2101 actatcattt tatggtgtat gcattggata cccggctcga tttggggacg aggtccgaca
     2161 aggcggatct ggaaaaggcg atgcaggggc atatcgttgc acagggagat ttggtagggc
     2221 tgtactcgaa agggaaataa gcagggacga cattcaggag gcg
//
LOCUS       DATGKE010000325         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1457722, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000325 DATGKE010000000
VERSION     DATGKE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457722"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VKU83_05610"
     CDS             complement(<1..388)
                     /locus_tag="VKU83_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HLI93060.1"
                     /translation="MYLLLRDNKQTGPYTLDELKAKGMKAYDLVWVEGRSAAWRYPSE
                     IDELRAFAPAVEEQPFDRFFKKPVTSPSTPAPAVAGEPLVSSAATVKRTIYVTMPGGV
                     PKQITREIPMREATPAREAAPLREAPS"
     gene            645..2375
                     /locus_tag="VKU83_05615"
     CDS             645..2375
                     /locus_tag="VKU83_05615"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HLI93061.1"
                     /translation="MDAAIQEKLTIWLNGNYDQATKDEIRRLQQENPNELTDSFYRNL
                     EFGTGGLRGIMGVGTNRINKYTVGMATQGFANYLKTSFPGKEVRVAIAHDSRNNSRFF
                     AETTANVFAANGIKVFLFQSLRPTPELSFAIRHLRCQGGVVCTASHNPKEYNGYKAYW
                     DDGGQLVPPHDKNVIAEVEKIGSIDEVKWGGGETNITMIGKDMDEAYIAMVRNLSVYP
                     EVIERQHNLRIVYTPIHGTGIKLVPEVLQRFGFTNVHVVDEQATPDGNFPTVIYPNPE
                     ESEAMSIGLRKAQQIDADILLGTDPDADRVGIGVKDRKGKWMLMNGNQTAVLAFNYLI
                     EARKAKGIAKANDMVIKTIVTTELIDEIARRSGVTCYNTLTGFKYIAELIKEKEWNEN
                     YVIGGEESYGLMIGSQIRDKDAVSAVALLCEMAAYEKDKGRTLYEKLTELYVQYGFYK
                     ESLISLTKKGMDGQKEIAAMMSGYRSSPPVTINGSAVVKLLDYELRTGKNLATGESWP
                     ITLPKSNVLQFILADGSKISARPSGTEPKIKFYFSVHTQLPSAAQFDRIDAELSARIE
                     GIIADMKLKS"
     gene            2379..3278
                     /locus_tag="VKU83_05620"
     CDS             2379..3278
                     /locus_tag="VKU83_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLI93062.1"
                     /translation="MKNLPRRKFLKMSGGAALAATLPRLPFAPDLAPPGSVAALPRIA
                     VQLYTVRQEFEKDIPGTLHKLAAMGIRHVETAFWPKNISLNQAARYIREAGLAVCSSH
                     VDIPAGDHKATVLATAEAFETKRVIWHGWPEDKRYSSADGTKELIGIYNEAGDWARSK
                     GLEFGLHNHWWEFRNRVGGRLVYEWLLDGVNKDVFFEIDTYWVKVAGQDPPAIVTKYG
                     DRAKFLHMKDGPAKYSQALVEDKPDPMVALGKGTQNVPAIAAAAKGHIEWMVIEMDVV
                     AGDVFGAIKESYDYLVNHRFARP"
     gene            complement(3323..4516)
                     /locus_tag="VKU83_05625"
     CDS             complement(3323..4516)
                     /locus_tag="VKU83_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI93063.1"
                     /translation="MVKIAMLGSGFIARFYAESILGQRSRDKIVAVYSRRKESADKFA
                     KDYHCNFSTTEMEAAIARPDVDMVCVSLPNNLHEIAVLLCCKHHKAVMTTKPLGRNAT
                     EARRMLEAVEDAGVFSAYLEDLVYTPKSIKAIESVKAGALGRILWAKSRETHPGPHSD
                     WFWDIEQAGGGCILDLGCHCVEIARTFIGKDIKPIEVMCWADTQVKPIDAEDHAIGLV
                     RYENGSIGQFEVSWTFRGGMDLRDEVMGTEGTIWINNCLRTGLEMFTTGKGADYVAEK
                     AESTSGWLFPMGDEMNDFGYNHMFTDVFRSFEQGKAPKETFYDGYVVNAILDSAYRSA
                     RTRQWEPVLLDIWRGKIGVTKDSHLTEYDPEHYLIKEETTHYGTKKVILKNKKDGKIV
                     ERTLS"
     gene            complement(4526..>5344)
                     /locus_tag="VKU83_05630"
     CDS             complement(4526..>5344)
                     /locus_tag="VKU83_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI93064.1"
                     /translation="YILVNFTNGLKPEQTAMPLYMTAAASLLLGIYSFTLPHTPPPAR
                     GKKVSARAIIGLDALKQLGSPSFYIFLICSFLICIPLAAYYNFTQIFLDSAHYQHIAA
                     TQTIGQVSETLFMILMPVFFVRYGVKWMLAAGMLAWVLRYALFAMGAPDLVNWMILTG
                     VALHGICYDFFFVTGQIYIDTKARVEIRAQAQGLIVFATYGVGMLVGAQIAGAVYDGF
                     LKDAKSLTLPQWQSFWWLPAIFAAVVLLIFIATFREKKATTAPVEKVENLVANV"
BASE COUNT         1260 a         1494 c         1458 g         1132 t
ORIGIN      
        1 tcgaaggggc ttcccgtagc ggcgctgctt ccctggccgg cgtcgcttcc cgcatgggga
       61 tctcgcgcgt gatctgcttc gggactccgc cgggcatcgt cacataaatg gtccgcttaa
      121 ccgtcgcggc gctggaaacc agcggctcgc ccgctacggc tggggcagga gtggacggcg
      181 aggtgacggg cttcttaaaa aaccggtcaa aaggctgttc ttctacagcg ggcgcgaatg
      241 cgcgaagttc atcgatttct gaaggataac gccaggccgc actgcggcct tctacccata
      301 ccaggtcata agccttcatc cccttcgcct ttagctcatc gagagtgtat ggcccggttt
      361 gcttgttatc acgtaagagc aggtacatca tgatccgtct ccggttatga ggtgttcaaa
      421 agaaatcagt acggtactgg attaagacga catcaaatat ttcaccggat attgtgaaga
      481 gtaagttata cttagatgct tataggcagg aggttgagag cgtttgcgcc aacaaatata
      541 aacattttcc gtcaggagta aaatagtgag gcggaagttt ctctggatat ttgctaatat
      601 tcccaatctt cccgaaattg gcctccaaaa cagaatacac gcaaatggat gctgctatac
      661 aggagaaact gactatatgg ttgaacggca actatgacca ggccacaaaa gatgaaatca
      721 ggcgcctgca gcaggagaat cccaatgaat tgacagacag tttttaccgc aatctcgaat
      781 ttggcaccgg cggccttcgc ggcatcatgg gagtaggtac caaccgcatt aacaagtaca
      841 cggtcggcat ggcgacccag ggattcgcca actatttgaa aacgtcattc ccgggcaaag
      901 aggttcgcgt cgcgattgcc catgacagcc ggaataacag ccgtttcttc gccgaaacga
      961 cggccaatgt attcgccgcc aacggaataa aggtttttct tttccagtcg ctccggccga
     1021 cgcctgagct cagcttcgcc attcggcacc tccgctgcca gggcggcgtc gtctgcaccg
     1081 cgtcccacaa tccaaaggaa tacaacggct ataaagccta ttgggacgac gggggccagc
     1141 tcgtgccgcc acacgacaag aatgtcattg cggaagtcga aaagatcggg agcatcgacg
     1201 aggtcaaatg gggaggcggc gagaccaaca ttaccatgat cggcaaggac atggatgaag
     1261 cctatattgc gatggtgcgc aacctttcgg tttatcccga agttatcgaa cgtcagcaca
     1321 acctaaggat cgtatatacg ccaatccacg gtacgggcat caagctggtg cctgaggtcc
     1381 ttcagcgttt cggctttacg aacgtccacg tcgttgacga acaggccact cccgatggca
     1441 attttcctac cgtcatttac ccgaatcccg aagaaagcga agccatgagc atcggtcttc
     1501 gcaaagctca gcagatcgac gcagatatcc tccttggaac cgaccccgac gccgaccggg
     1561 ttgggatcgg ggtgaaagac aggaagggca aatggatgct gatgaacgga aaccagaccg
     1621 ccgtccttgc ctttaactat ctgatcgaag cgcggaaggc taagggcatc gccaaagcga
     1681 atgatatggt cattaaaacc atcgttacga ccgagctgat agacgagatc gcgcgccgaa
     1741 gcggggtcac ttgttacaat acgctgacgg ggttcaaata catcgcggag ctgataaagg
     1801 aaaaggaatg gaacgagaat tacgtcatcg gcggagaaga aagttacggg ctcatgatcg
     1861 gaagccagat ccgcgataaa gatgccgtca gcgccgttgc cctcctttgc gaaatggccg
     1921 cctatgaaaa agacaagggc cgcacccttt acgagaaatt gaccgagctt tatgttcaat
     1981 atggctttta taaggaatcg ctgatctccc tcacgaaaaa aggaatggat ggccagaaag
     2041 agatcgccgc catgatgagc ggctatcgga gcagtccgcc cgtaaccatc aacggctcgg
     2101 cggtcgtaaa gctgctggac tacgagctcc gtacagggaa gaacctggcc accggtgaat
     2161 cgtggccgat caccctcccg aaatcgaatg tcctccagtt tattctcgct gatggcagca
     2221 agatctcggc ccggccttcc ggcacggaac cgaaaattaa gttctatttc agcgtccaca
     2281 cgcaattgcc ttcggccgcg caattcgacc ggatagacgc ggaactttcc gccaggatcg
     2341 aaggtattat cgccgacatg aaactaaagt cttgaaatat gaaaaacctg ccccgcagga
     2401 aatttctgaa gatgagcggc ggcgccgccc tggcagccac cctgccgcga ttgccatttg
     2461 cgccggacct cgcgccgccg gggagcgtcg ctgcgcttcc gcgcattgcc gtgcagctgt
     2521 atacggtgcg ccaggaattt gagaaagata ttccgggaac ccttcacaag ttggcggcca
     2581 tgggcatccg gcatgtggaa acagccttct ggccaaaaaa tatcagcctt aaccaggcgg
     2641 cccgttatat tcgcgaggcg gggctcgctg tttgttcaag ccacgtagac attccggcgg
     2701 gcgatcacaa agccaccgtc ctggcgacgg cggaagcgtt tgaaacaaaa cgggtcattt
     2761 ggcacggctg gcccgaagac aagcgttaca gcagcgccga tggcacgaag gaattgatcg
     2821 gtatctacaa cgaggcaggc gactgggcga ggtccaaggg ccttgaattc ggcttgcaca
     2881 atcattggtg ggaattcagg aacagggtag gaggccgcct ggtgtacgag tggctgctcg
     2941 atggcgttaa caaggacgtc ttcttcgaaa ttgacaccta ctgggtgaag gttgcaggcc
     3001 aggatccgcc cgccatcgtt acgaaatacg gcgatcgcgc aaaattcctt cacatgaagg
     3061 acggccccgc caaatatagt caggccctgg ttgaagacaa acccgatccc atggttgccc
     3121 tcggcaaagg gacacagaac gttccggcga ttgccgccgc agccaaaggc catatcgagt
     3181 ggatggtcat cgaaatggac gtcgtggccg gggatgtttt tggcgcgatc aaagaaagct
     3241 acgactacct ggtgaaccat cggttcgcac ggccctgatt tttcaccaac cgccctccgt
     3301 catcgccggc cgcatgcgct tctcagctca gtgtccgctc aacgatcttg ccgtcctttt
     3361 tgtttttcag gatgaccttt ttcgtgccat agtgtgtcgt ctcttccttg atcaagtagt
     3421 gttccgggtc gtattccgta aggtggctgt ccttggtaac gccaatcttg ccacgccaga
     3481 tatccagcag caccggctcc cattgacggg tcctcgccga gcggtaagcc gagtccagga
     3541 tggcgttgac cacgtagccg tcgtagaagg tctccttcgg agcctttcct tgctcgaagg
     3601 acctgaaaac gtcggtaaac atatggttgt aaccaaagtc attcatctcg tcgcccatcg
     3661 ggaaaagcca gccactcgtg ctctcggcct tctcggcaac gtaatcggcg cctttcccgg
     3721 tcgtgaacat ttccaatcct gtcctcaggc agttattgat ccagatcgtc ccctcagttc
     3781 ccatcacctc gtcgcggagg tccatgccgc cgcggaatgt ccagctcact tcgaattgac
     3841 cgatggagcc gttctcatat ctgaccaggc cgatggcatg gtcttccgcg tcgatcggct
     3901 ttacctgggt gtctgcccag cacatgacct cgattggttt gatatccttg ccgataaagg
     3961 tccgggcgat ctcaacgcag tgacaaccca ggtcgaggat acagcctccg cccgcctgtt
     4021 cgatatccca gaaccaatca ctatgcggtc cgggatgtgt ctcccgcgat tttgcccaca
     4081 ggatccttcc cagcgccccg gccttgacgc tttcgatggc tttgatggat tttggcgtat
     4141 agaccaggtc ctccaggtaa gcactgaata ctccggcgtc ctccaccgcc tccagcatcc
     4201 gccgcgcctc cgtggcgttc cggccgaggg gcttcgtcgt catcacggcc ttatgatgct
     4261 tgcagcataa caggacggcg atctcatgga gattgttggg gagcgagacg cacaccatat
     4321 cgacgtccgg ccgcgcgatc gccgcctcca tttcagtcgt cgaaaaatta caatgatagt
     4381 ctttggcgaa tttgtccgcg ctttccttgc ggcgcgaata cacggccacg atcttgtccc
     4441 ggctccgttg gccaaggatc gattcggcgt agaaccgggc aataaagccc gagccgagca
     4501 tggcaatctt taccatatga aagttttaaa cgttagctac gaggttttct actttttcaa
     4561 ccggggcggt cgttgccttt ttttcccgga atgtggcgat aaatatcaag agcaccaccg
     4621 cggcgaatat ggcgggcagc caccaaaaac tttgccactg cgggagggtg agcgatttcg
     4681 catccttcag gaacccgtca tagaccgcgc cggcgatctg cgctcccacg agcattccga
     4741 ctccgtaggt cgcaaatacg atcaggccct gggcctgcgc ccggatctcg accctggcct
     4801 tggtatcgat gtagatctgg ccggtgacaa aaaagaaatc gtaacagatc ccgtgcagcg
     4861 caacgcccgt caggatcatc cagttcacca aatccggcgc acccatcgca aacagcgcat
     4921 agcgcagcac ccaggccagc attcccgcgg ccagcatcca tttcacacca tagcggacaa
     4981 aaaagacggg catcaggatc ataaacaagg tctcggacac ctgcccgatg gtttgcgttg
     5041 ccgcaatatg ctggtagtgg gcgctgtcca ggaagatctg cgtaaaattg tagtatgcgg
     5101 ccagggggat gcagatcagg aaggagcaga tcagaaagat atagaaggac gggctcccca
     5161 actgtttcaa ggcgtcaagt ccgatgatcg cgcgggcgga taccttcttg ccgcgcgccg
     5221 gggggggcgt atgcggcagg gtaaaactat agatccccag caacaggctc gctgcagccg
     5281 tcatatacaa cggcatggcc gtttgctcgg gtttgagtcc gttggtgaag tttacgagga
     5341 tata
//
LOCUS       DATGKE010000326         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_39976, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000326 DATGKE010000000
VERSION     DATGKE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39976"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1282
                     /locus_tag="VKU83_05635"
     CDS             98..1282
                     /locus_tag="VKU83_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HLI93065.1"
                     /translation="MQATTQLIHSIPVDPLTGAIAVPIYQTSTFVQDAPGVNKGFDYA
                     RTGNPTRATLENLVAQLEGGSTGVAFGSGLAAIDAVVKLLESGDEIVAVDDIYGGAFR
                     LFEQVYRKFGITVRYVDTSSVERVLDAITPKTRLIWVETPTNPTLKITDIRAIAALSR
                     AYGCLLCVDNTFASPALQKPLSLGADIVIHSGTKYLGGHSDLIAGLVVTADAELGQRV
                     KFLQNATGAILGPFDSWLLIRGIETLHLRVRQHCSNALAIAEFLQQHPAVDKLYYPGL
                     PDHPGHAIAAAQSKGFGGIVSFSLKAGTVAAATAVVTSTRLFKLAESLGGIKSLVSHP
                     AQMTHKTIPAERRRAAGVSDSLIRLSAGLEEAEDLVDDLRQALDNLAFKASKRQSLHI
                     EC"
     gene            1287..2543
                     /locus_tag="VKU83_05640"
     CDS             1287..2543
                     /locus_tag="VKU83_05640"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLI93066.1"
                     /translation="METHKQLTIGLFGFGVVGEGLYKVLQRTPSLKATIRRVCIKHPD
                     KKREAPAELFTTDRDLILNDPEINVVVEVINESGPAFQIVSRALRSGKSVVSASKKMI
                     AEHLPELLQLQQETGASFLYEAAACASIPVVRNLEEYYDNDLLHSIKAIVNGSTNFIL
                     TKMFEEKLGFQEALLLAQQLGFAESDPSLDVEGYDAANKWTFLLTHAYGIVGSADDLV
                     FSGIQHIQAGDAAVAREKHFDIKLVAQAKKLENGKVAAFVLPQFVPHDDHLAFVRNEY
                     NGVVIESGFADKQFFYGKGAGSFPTASAVLSDLSALRYQYKYEYKKLYHHTPHQLTED
                     FYVRVYVSFDDWKYIPRDRFEWIEEWHAESDRKYLIGVIHFRELANETWWKEHKTSLI
                     LTPEPVIEDVEIRKLKKKSLELAGVI"
     gene            complement(2540..>2982)
                     /locus_tag="VKU83_05645"
     CDS             complement(2540..>2982)
                     /locus_tag="VKU83_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93067.1"
                     /translation="GLPFTGAEVLQVCDTAHFTEVVRQLPQGLESDIREKGVNLSGGQ
                     KQRLALARGILAARDSSLVLLDEPTSSVDPRTEALIYERLFAAFADKAIISSIHRLHL
                     LPHFDYVYLLHHGRVIAEGSFDELRTHNAAFRELWRHQQEASRS"
BASE COUNT          643 a          829 c          906 g          604 t
ORIGIN      
        1 agctcttctg acagatcaga agagcttttt ttttctgatc atctcttggt aaccggtgtc
       61 gctgaacaaa cttttttaac ctgaaaaaat ttgtaagatg caagcgacaa cacagctcat
      121 tcacagtatt cccgtggacc ctctgaccgg cgccatcgcg gtgcccatct atcagacttc
      181 gacttttgtt caggacgcgc cgggcgtcaa caagggtttt gactatgccc ggaccggcaa
      241 tcccaccagg gccacgctgg aaaatttggt ggcgcagctg gaaggcggca gcaccggcgt
      301 ggcattcggc agcgggctgg ccgccatcga cgccgtggtt aaactgctgg aaagcggcga
      361 cgagatcgtg gcggtggacg acatttatgg cggcgccttt aggttattcg agcaggtgta
      421 ccggaaattc ggcatcacgg tccgctatgt ggacacttcc agcgtggaac gcgtcctgga
      481 tgccatcacg ccgaagaccc ggctgatctg ggtggagacg ccgaccaatc ccacgctgaa
      541 gatcacggat atccgggcga tcgcggcgct ttccagggct tatggctgtc tgctttgcgt
      601 ggacaatacc tttgcctcgc cggcgctcca gaagccgctt tccctgggcg ccgacatcgt
      661 gatccattcc ggcaccaaat acctcggcgg ccacagcgac ctgatcgctg gactggtggt
      721 gacggcggat gccgagctgg gtcagcgggt gaaattcctg cagaatgcaa ccggggcgat
      781 ccttggcccg ttcgacagct ggctgctgat ccggggcatc gagacccttc acctgcgggt
      841 gcgccaacat tgcagcaacg ccctcgccat cgccgaattc ctgcaacagc atccggcggt
      901 ggacaagctg tactatccgg gattgccgga ccatcccggg catgcgattg ccgccgcaca
      961 gtcgaaggga tttggcggca tcgtcagctt ctcgctcaaa gcggggactg tggcggcagc
     1021 gaccgcggtg gtgacgtcga cgcgcctctt caagcttgcc gaaagccttg gggggatcaa
     1081 gagcctggtg tcgcacccgg cgcagatgac gcataaaacc atcccggcgg agcggcgcag
     1141 ggcggccggg gtctccgaca gtcttatccg gttgtcggcg ggactcgaag aagccgaaga
     1201 cctggtggac gacttgcggc aggcgttgga caatttggct tttaaggcat cgaagcggca
     1261 atcattacac atcgaatgtt agaaatatgg aaacacataa gcagctcact atcggactat
     1321 ttggatttgg cgtcgtcggg gaaggcttat acaaggtttt acagcggaca ccgtcgctga
     1381 aggccacgat caggcgcgtt tgtatcaagc atccggacaa aaagcgggaa gcgccggcag
     1441 aactgttcac gacggaccgc gacctgattt tgaatgatcc ggagatcaac gtggtcgtgg
     1501 aagtgatcaa tgaatccggc ccggccttcc agatcgtatc ccgggcgctg cgcagcggca
     1561 agtcggtcgt cagcgccagc aagaaaatga tcgcggaaca cctgcccgag ctgttacagc
     1621 tccagcagga gaccggcgcc tcctttttat acgaggctgc ggcctgcgca tcgatccccg
     1681 tagtacggaa cctcgaggag tattatgata atgacctcct gcattcgatc aaggccatcg
     1741 ttaacgggag cactaatttc atcctgacaa agatgttcga ggaaaagctg ggattccagg
     1801 aggctttatt gctggcccag cagctgggat ttgcggaaag cgacccctcg ctggacgtgg
     1861 agggttatga cgcggccaat aaatggacct ttttactgac ccatgcctac ggcatcgtcg
     1921 gatcggccga cgatcttgtc ttcagcggca tccagcatat ccaggccggg gacgcggcgg
     1981 tcgcgcggga aaagcatttc gatatcaaac tggtggcgca ggccaagaag ctggagaatg
     2041 gcaaggtcgc cgccttcgtg cttccgcagt tcgtcccgca cgacgatcac ctcgctttcg
     2101 tccggaacga atacaacgga gtggtgatcg aaagcggctt cgccgacaag cagttcttct
     2161 atggaaaggg cgccggctcc ttccctactg cctcggccgt cttaagtgat ctctccgccc
     2221 tgcggtacca gtacaagtat gagtacaaga aattatacca tcatacgcct catcagctga
     2281 cggaggactt ctatgtccgg gtgtacgtca gtttcgacga ctggaagtat attccgcgcg
     2341 accgtttcga gtggatcgag gagtggcatg cggagtccga ccggaaatac ctgatcggcg
     2401 tcattcattt ccgcgagctg gcgaacgaaa cctggtggaa agaacacaag acctctttga
     2461 tcctgacgcc cgaacccgtg atcgaagatg tggagatccg caaactgaaa aagaagagcc
     2521 tggagcttgc aggcgtgatt taagagcggc ttgcttcctg ctgatgccgc cacaattcgc
     2581 ggaaggcggc gttatgcgtc cggagttcgt caaaactgcc ttcggcgatc acccggccat
     2641 gatggaggag ataaacgtag tcgaaatggg gcagcaggtg caggcggtgg atagacgaga
     2701 tgatggcttt gtcggcgaac gcggcgaaaa ggcgttcgta gatcagggcc tcggtcctcg
     2761 ggtctacgct gctggtgggc tcatccagca gcaccaggct gctgtcccgg gcggcgagga
     2821 taccccgggc gagggctaac cgctgtttct ggccgcctga aagattgacg cccttttccc
     2881 ggatatccga ttcgagtccc tggggaagct ggcggactac ttccgtgaaa tgggcggtgt
     2941 cgcaaacctg caagacctcc gccccggtga aggggaggcc aa
//
LOCUS       DATGKE010000327         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_956948, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000327 DATGKE010000000
VERSION     DATGKE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956948"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VKU83_05650"
     CDS             <1..248
                     /locus_tag="VKU83_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93068.1"
                     /translation="SVSGLLSRDFLRPVVLSILIACPLAWWVMEKWLQNFAYRTPISW
                     WIFPEVGGGLLLIALGTVLFRTIRAAQANPTINLRNE"
     gene            complement(286..1011)
                     /locus_tag="VKU83_05655"
     CDS             complement(286..1011)
                     /locus_tag="VKU83_05655"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93069.1"
                     /translation="MKKLALFPFLLLLAITCWCQDCSQYLLLQNNKTIETTIYNKKNE
                     PVGKRVSTVSNVTNSGGATTATINTQMFDKKGNSTGTSTSNMKCTGGVFQLDMKLILP
                     EGPAEKMSSAKVTGGEGIVEYPAGMKAGDTLRSANLVLSNSNLPPPPAGGAPGPPPPP
                     LFGGSNLSMVIWDRKVEGQESVTTPAGTWNCFKISYKSRVSYRSGPLPVNHTADVTEW
                     YAPGVGVIKTESKDGSTAVTSIK"
     gene            1185..1487
                     /locus_tag="VKU83_05660"
     CDS             1185..1487
                     /locus_tag="VKU83_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLI93070.1"
                     /translation="MSAQKVMIGVLSGLLAGVAIGILIAPAEGMETRQRISDTADSIK
                     RKLRRLRGITTDELDELKDIFERETSGMRDDVRSRVLNLIKAAKDKGNHIKEEALS"
     gene            1814..>2139
                     /locus_tag="VKU83_05665"
     CDS             1814..>2139
                     /locus_tag="VKU83_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HLI93071.1"
                     /translation="MPVDLRSDTVTRPTQGMLDAMMHAEVGDDVFGEDPSINRLEKMA
                     AGLFGMEAALYCPSGTMSNQIGINCHTRPGDEVICERSSHVYVYEGGGIAFNSGSQVR
                     TLEGDRG"
BASE COUNT          467 a          549 c          603 g          520 t
ORIGIN      
        1 ggtcggtcag cgggttactg tcgcgggatt ttctgaggcc ggtcgtgctg tctattttaa
       61 ttgcctgtcc tcttgcctgg tgggtgatgg aaaaatggct gcaaaacttc gcctaccgca
      121 cgccgatcag ctggtggata ttcccggagg tcggcggcgg cttgttgctg atagcgcttg
      181 ggactgtttt attccgcacg atcagggccg cgcaggcgaa cccgacgatc aacttgcgaa
      241 atgaataagg gtccgcgatt gcggcaaccg gcgtggcggc taatgttatt tgatcgatgt
      301 caccgctgtc gagccatctt ttgactcggt cttgatcacg ccgacgccgg gggcgtacca
      361 ttccgtaacg tctgcggtgt ggttgaccgg aagcggtccc gacctgtaac tgaccctgct
      421 tttgtaactg atcttaaagc aattccaggt gccggccggc gtagtaaccg attcctgccc
      481 ttcgaccttg cggtcccaga tcaccatgga aaggttggat ccgccgaaaa gcggcggggg
      541 tgggggacca ggagcgccgc ctgccggtgg cggaggaagg ttgctgttgg acaagacgag
      601 gttggcgctt ctcagcgtgt cgcccgcctt catcccggca ggatattcga caatcccctc
      661 gccgcccgtt accttcgccg aactcatttt ctcggccggc ccctcgggca ggatcagttt
      721 catatccagc tggaaaacgc cgccggtaca cttcatgttg ctcgtggacg tgcctgtcga
      781 attacctttc ttgtcaaaca tctgggtgtt gatcgtggcg gtagtagcgc ccccgctgtt
      841 ggtgacgtta gataccgtgg agacccgctt cccgaccggt tcgttcttct tattgtagat
      901 ggtcgtttcg atggtcttgt tgttttgcaa cagaagatat tgagagcagt cctggcacca
      961 gcaggtgatc gccagcaaaa gcaggaatgg gaatagggcg agctttttca tattaaaaaa
     1021 tatttgtatc aggaagatag gcaatttttc actgccgcca ataccacaaa tgggtataat
     1081 tttacccacg ccgcccgtga ttatcaatag gaaacggttg atatattccg gcattgttct
     1141 tgaaaataaa acggtattgt attgttcact taaaactatt cactatgagt gcgcagaaag
     1201 taatgatcgg agtcttatcc ggacttctgg caggtgtggc cattggcatc ctcatcgcac
     1261 cggccgaagg tatggaaacc cgccagcgga tctcggatac tgccgactcc ataaagcgaa
     1321 agcttaggag gttgagaggg atcacgaccg acgagttgga tgaattgaaa gatattttcg
     1381 aaagagagac ctcggggatg cgcgacgacg tgcgtagccg agtcttaaac ctcatcaaag
     1441 ccgcaaagga caaaggaaac cacattaagg aagaagcact ttcttagctt accgatgaat
     1501 cacggatgag ggagccaaca ggctccttcc ccgcaaggcc tcgccttgcg gacattgtga
     1561 aaaaaaggag cctgtacttt ttggagccaa caggctcctc ccccgcaagg cctcgccttg
     1621 cggacattgt gaaaaaaagg agcctgtact ttttggagct gtcaggctcc ttcccgcaag
     1681 gcctcgcctt gcggacattg tgaaaaaaag gagcctgtac tttttggagc caacaggttc
     1741 ctttttttat ttggcggacg gcccccatat gctcatttcc cattaatttg cgacctcatc
     1801 caatgtattg tctatgcccg tcgaccttcg ttccgatacc gtcacccgcc cgacccaggg
     1861 aatgcttgat gctatgatgc atgctgaagt cggggacgat gtttttggcg aggatccctc
     1921 tattaatcgt ctcgagaaaa tggcggccgg gctcttcggg atggaagccg cgctgtattg
     1981 tcccagcggg acgatgtcaa accagatcgg catcaattgc cacacccggc cgggggatga
     2041 agtgatctgt gagcgaagct ctcacgttta tgtgtacgaa ggcggcggca tcgcgttcaa
     2101 ttcggggtcg caggttcgca cgctggaagg cgaccgggg
//
LOCUS       DATGKE010000328         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_317683, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000328 DATGKE010000000
VERSION     DATGKE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317683"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2400
                     /locus_tag="VKU83_05670"
     CDS             <1..2400
                     /locus_tag="VKU83_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93072.1"
                     /translation="ARVWLPIAMLRNYFKSAWRGILRGRSFSIINILGLIVGMAGATL
                     ILLWLINEISYDRFHVNRDRIYQVYAMTDIPGEKHTCIGVVSQPLGPAMKARFPDVES
                     FARLTDVDHFLVTTGGKSFTKLKGDFVDPDFLRIFTFPLVAGDRNDPLGSVNSILLTE
                     RLARKLFGTADAVGRTVRLDSVDNFTVTGVLKDLPANTEFDFEYLLPWSYLKKLGWNN
                     DNWISNNSYTFLLLRPHTDVGAFNLKIRDLTRINTGRKDLWVHFVYPLSQWHLYSEFD
                     NGIPAGGRIDTVRVFALIAAFILLIACINFMNLSTARSERRAKEVGIRKVAGAGRGLL
                     IGQFMAESFLTVFVSGIIALAAVQWLLPAFGRLAGTPLAVPYGSAAFWLGAGAFLLMT
                     SVLAGAYPAFYLSAFKPVSIFRKEFRRAPAALSARKVLVVLQFCFAILLIISTLVVRD
                     QLQYTKDRDAGYSRNNLIYVEFEGDIERNYPLIRQDLLQSGIAVAVSKNFQDIADGGP
                     YTWGYRWPGEPPHDTTIGIRMFSTDAGLVHTMGMHLLAGRDIDIYQHPTDSFALILNE
                     TAVKWMGFQDPIGQIIYNPYEHTRWHVVGVVRDYVNGTPYGRVPPVMIQGPASTFTTM
                     HIRFNPANSTSASLEKAAAIFKRYNPAYPFDYRFVDQEFAGQFEDAQRTKTMAGLFAA
                     LAIFISCLGLFGLSAFVAESRFKEIGVRKVLGASASGIARLLSADFIRLVLIAFAIAA
                     PIAWDVMNRWLANFDYRISLGWAVFALAGFLAVAIALLTVSFQAVRAASVNPVDSLRA
                     D"
BASE COUNT          509 a          761 c          734 g          626 t
ORIGIN      
        1 gcaagggttt ggctgcccat cgctatgctc cgtaattact ttaagagcgc ctggcgcgga
       61 atccttcgcg ggcgaagctt ttccatcatc aatatcttag ggctgattgt cgggatggcc
      121 ggtgcaacgc tgatcctgct atggctgatc aacgagatca gctacgaccg gttccacgtt
      181 aaccgggacc gcatctacca ggtctatgcc atgaccgata tcccgggaga aaaacatacc
      241 tgcattggcg tcgtatccca gcccctgggg ccggcaatga aagcccggtt ccctgacgtc
      301 gaatctttcg cccggctaac cgatgtggac catttcctgg ttaccaccgg cggtaagagc
      361 ttcacgaaac tcaagggtga tttcgtcgat cccgacttcc tgcggatctt tacgtttccg
      421 cttgtggcgg gagatcgcaa tgatccgctt ggctcggtga attcgatcct tttaacggag
      481 aggcttgccc gaaagctgtt cggaacggcg gacgccgtgg ggaggaccgt gcggctcgac
      541 agcgtagaca atttcacggt gaccggcgtc ctgaaagacc tgccggccaa tacggaattt
      601 gatttcgaat acctgctgcc atggtcatac ctgaagaaac tgggctggaa taacgacaac
      661 tggatcagca acaacagcta tacgttcctg ctgcttcgcc cccatacgga tgtgggcgcc
      721 ttcaatctca agatcaggga tctgacgcgc atcaataccg gccggaaaga cctttgggtg
      781 cactttgtct acccgctcag ccaatggcat ctttactccg aatttgacaa tggcataccc
      841 gcaggagggc ggatcgatac tgtccgggta ttcgcactta tcgcagcttt catcctgctc
      901 atcgcctgca ttaacttcat gaatttaagc accgcaagga gtgagcggcg cgcgaaggaa
      961 gtcgggatcc ggaaggttgc gggggcgggg cgcggtttgc tgatcgggca attcatggct
     1021 gaatcgtttt taacggtgtt cgtctccggt atcatagcgc ttgccgccgt gcagtggctt
     1081 cttcccgctt ttggccggtt ggcgggaacg ccgctggcgg ttccgtatgg ttccgcggcc
     1141 ttttggctcg gcgccggtgc atttttattg atgaccagcg tactcgcggg agcctatccc
     1201 gctttctatc tctcggcctt caaaccggta agcattttcc ggaaggagtt ccgccgggcg
     1261 ccggcggcct tatccgcaag gaaggtcctg gttgtgctgc agttttgttt tgcaatcctg
     1321 ctcatcattt caacactggt tgtccgcgac cagttgcagt ataccaagga ccgggatgcc
     1381 ggatattcaa gaaataatct tatctacgtc gaatttgaag gtgatattga aagaaactat
     1441 cccctcatcc gtcaggacct tcttcaatcc ggcatcgcgg tcgccgtgag caagaatttc
     1501 caggatatcg ccgacggcgg cccttacacg tggggatacc gctggccggg ggagccgccg
     1561 catgacacga cgatcgggat caggatgttt tccacggacg ccggtcttgt ccataccatg
     1621 ggaatgcatc ttttggccgg ccgcgacatc gacatctatc agcacccaac ggatagcttt
     1681 gcccttatcc ttaacgagac cgccgttaaa tggatgggct tccaggatcc gatcggccaa
     1741 attatctaca atccatacga gcacacccgc tggcatgtcg tcggtgtagt ccgggactat
     1801 gtcaatggta caccgtatgg ccgggtgcct cccgtgatga tccagggacc cgcgtccacg
     1861 ttcacgacca tgcatatccg ctttaatccg gcaaattcca catcggccag cctggaaaag
     1921 gccgcggcga tcttcaaacg atacaatccc gcctatccct tcgactaccg cttcgtcgac
     1981 caggaatttg cgggccaatt tgaagatgcg cagcgcacga agaccatggc cgggctattc
     2041 gccgcgttgg ccatcttcat ttcctgtctc ggcctctttg gcttgtcggc cttcgtcgcg
     2101 gaaagccgct tcaaggagat cggggtccgc aaggttctcg gtgcttccgc gtccgggatc
     2161 gccaggctgt tatccgccga cttcatccgg ctcgtgctga tcgcgtttgc gatcgctgcg
     2221 ccgatcgcat gggatgtgat gaatcgctgg cttgcgaatt tcgactaccg gatttcgttg
     2281 ggctgggcgg ttttcgccct cgcgggtttc ctcgccgttg ccattgccct cctgacggtg
     2341 agcttccagg cggtccgggc cgcttccgtg aatcccgtcg acagtttgcg ggcagactga
     2401 gtatgcccgg accgcccggc ggggctaatg attcgccttc ggcggattgg cctgcggcca
     2461 atcggggctt acgtcccaac cctgcatttg ggctcgtgcc gcaccggaaa attgcacgag
     2521 ttggcaataa aacacttctt ggccgcttcc cggtgcagct ccatcgccct gggaatccgc
     2581 gccgcatccg taatagttgc aatgggatat aaggtaacct ccttgaaata
//
LOCUS       DATGKE010000329         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1254353, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000329 DATGKE010000000
VERSION     DATGKE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254353"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..605)
                     /gene="msrB"
                     /locus_tag="VKU83_05675"
     CDS             complement(69..605)
                     /gene="msrB"
                     /locus_tag="VKU83_05675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLI93073.1"
                     /translation="MKYSSLLAAALLFAFTLPAAAQSRNIRYPDPPVASPNTKYPMTL
                     TDAQWKARLTPEEYYILRQAGTERAFTGKYDHFYQKGTYYSAASLQPLFSSDTKFDSG
                     TGWPSFYAPISPDAVRLVLDHSDGMTRWEVVDSKSGSHLGHVFDDGPAPTGKRYCMNS
                     AALIFVPAGGAPPTSSKP"
     gene            complement(781..1590)
                     /locus_tag="VKU83_05680"
     CDS             complement(781..1590)
                     /locus_tag="VKU83_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93074.1"
                     /translation="MSSTVTSPELSLATADSIPAPRALPSAGQVFLSLFKADLTTQWR
                     NRRASLMVVLLPVVIVMSWKALIARTGGAFVLANAATLGLNAIGLMGYPTTIAKDRDK
                     DIFQRLRVAPAPGWTIMGSRLAVQLVMILFMIIVVFLVGYQYDNIVLPVTGYLAALLA
                     SIAGGAVYLGLGQAIVGRIRNAETVTSTCRLIYIAFIMVGMFGELHMLGKYIDDITPY
                     TPYGTVKTIVSAAMTPAEWNSTSTLALFITLGYTAVFTYAGIRWFKWSTKS"
     gene            complement(1621..2403)
                     /locus_tag="VKU83_05685"
     CDS             complement(1621..2403)
                     /locus_tag="VKU83_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93075.1"
                     /translation="MNTSTSLPVLRVENLDIFYGKFQAVRNVSFEVRPGEIFGLLGPN
                     GAGKTSTLSACEGLIKASSGTILVAGYDIRHQPIYARASMGVQLQSTSFQPELTVGEI
                     ITLYAGLYGVHLTQEGLMAILGDIKLADQVGKRFGQLSGGQQQRVSLVIATIHHPKLV
                     LMDEPTTGLDPQSRRQLWERIEAMRDKGHGILLTTHSMEEAESVCDRIAIIDHGRIMT
                     TDTPQAIIDDNRDNPDVIQASRKGKITLEDVFIGLTGTAIRS"
     gene            complement(2708..3133)
                     /locus_tag="VKU83_05690"
     CDS             complement(2708..3133)
                     /locus_tag="VKU83_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93076.1"
                     /translation="MNTNKKNHAVRIIAVFSTAVMFLIGSPMASFANAGGARKAATAT
                     NAQVSVTYQGVTNNDVVFKVDYENPTGEKFWLVIKNDNGDVVFSQQFDDVHFSKNVRF
                     LNTDADINPTFIVRTANSDVVREFQVNNIIPETTMVTGL"
     gene            3402..>5171
                     /locus_tag="VKU83_05695"
     CDS             3402..>5171
                     /locus_tag="VKU83_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93077.1"
                     /translation="MKRIIIILLTLAPMAATLQAQDNPLWMRYPAISPDGQTILFSYN
                     GDIYSVPSRGGQAMPLTISESYEFSPVWSHDGKWIAFASDRYGNFDVFVMPATGGVAK
                     RLTYFSGNEVPSSFTGDDKSILFSSLRQDLVSNAQFPGQMPQLFSVPVTGGRVTQVLA
                     VAAPEAKANAAGDKLIFEDQKGYENEWRKHHTSAITRDIWLYDLRSGKFQQLTSFRGE
                     DRSPVIGSDDRDFYYLSEQDGTMNVYRDNLAGPGKPLQLTHFARNPVRFLTVSNDNTL
                     CYGYDGEIYTQRPDGQPQKVAIRIAEDGRNEIEKVLPVNGGVTEMKMSPNGKEIAFVF
                     RGEIFVTSVDGGITKRVTSTPWQERSVGWSPDGRSLIYAAEKDHNWNIYTTSISRKEE
                     PYFYASTVLKVETVVATPAEEFQPAFSPDGKEVAYLEDRTTLKVINLATKKSRVVLPA
                     DKNYSYADGDQYYQWSPDSKWFLVQFGPPERVMTPEVGLVAADGKTPVVNLTLSGYDD
                     IMPKWSMDGKMMIWGSDREGARQQAGYPVTADVYGMFFTKDAFDRFKLSKEDLALVKE
                     AEAKEKAEADKKKAGKDTANAKKD"
BASE COUNT         1209 a         1257 c         1583 g         1122 t
ORIGIN      
        1 gtctggccac aagcggcgag caggaaaacg gcgcaaaagg tgaagagggc gtgtttcata
       61 taaagcgttt aaggttttga gctggtcgga ggcgcacctc cggcgggaac aaagatgagc
      121 gctgcggagt tcatgcaata acgtttcccg gtcggcgcgg gaccgtcgtc aaacacatgc
      181 cccagatggg agccgctctt tgagtcgacc acttcccacc tggtcatgcc gtcactgtgg
      241 tccaggacaa ggcgaaccgc gtccgggctg atcggggcat aaaagcttgg ccagccggtg
      301 ccgctgtcaa attttgtatc cgaactgaat agcggttgca gcgaggcggc ggaataatag
      361 gtccctttct gatagaaatg gtcgtatttg ccggtgaatg ccctttcggt gcccgcctgg
      421 cgaaggatat aatattcttc gggggtcaac cgggccttcc actgcgcgtc tgtcagggtc
      481 atcggatatt ttgtattggg ggaggcaacg gggggatcgg gatacctgat gttcctggac
      541 tgggccgcgg cggggagggt aaaggcgaac agaagggcgg ccgcgagaag gctcgaatat
      601 ttcatggcgg tgctgttttt ctgtaaataa acgaaggaaa tggcaaagag gtttggcagg
      661 gcttgttaag attccggcgg cgatccgctc cggaagtcac aagcttttga gttccagggt
      721 attccttcgc ttatgaatca cgcctgggcc atccggtcgg cggcagtctg tcactccagg
      781 tcagcttttt gtcgaccatt tgaaccagcg gatgccggcg taggtgaaaa cggcggtata
      841 gccaagcgtt atgaacaggg ccagggtgct ggtgctgttc cattcggcgg gcgtcatggc
      901 tgcggaaacg atggttttga ccgttccata gggagtatag ggggtgatgt catcaatata
      961 ttttccaagc atgtgcaatt cgccgaacat tcctaccatg ataaaggcga tataaatgag
     1021 ccggcaggtt gaagtaaccg tttctgcgtt tcggatcctg ccgacgatcg cctggcccag
     1081 gccgagatat actgcgccgc cggcgatgga agcgagaagc gcggccaggt agccggtgac
     1141 cggcaacact atattgtcgt actggtagcc aacgagaaat accacaatta tcataaaaag
     1201 gatcatcacg agctgaacgg ccaggcgact acccatgatc gtccaaccgg gtgcgggcgc
     1261 cacgcgcagt cgttgaaaga tatctttgtc ccggtcctta gcgatcgtgg tgggatatcc
     1321 catcagaccg atggcgttca ggccaagcgt cgcggcatta gccagaacaa aagcaccgcc
     1381 ggttcgggcg atgagtgctt tccaggacat tacgataaca accggcaaca gcacgaccat
     1441 cagggatgcc cgccggttgc gccattgagt ggtaagatct gccttgaaaa gggaaaggaa
     1501 gacctgtccg gcggaaggca gggcgcgggg cgcgggaata gaatccgcgg tggcgaggct
     1561 gagctcgggt gaagtgacgg tgcttgacat gatacgaaat tttattgtaa tggtcctttg
     1621 ttagctgcgt atggccgtcc ccgttaggcc gataaatacg tcctcgagcg tgatcttccc
     1681 cttccgggag gcctggatca catcagggtt gtcacggttg tcgtcgatga tcgcctgggg
     1741 cgtgtcggtg gtcatgatcc ggccatggtc gatgatggcg atgcgatcac aaaccgattc
     1801 tgcttcttcc atcgaatgcg tggtcaggag aatgccgtga cctttgtcgc gcatggcttc
     1861 gatcctttcc caaagctggc ggcgggactg ggggtcaagg ccggttgtgg gttcgtccat
     1921 gaggacaagt ttgggatggt ggatggtggc aatgaccagg gagacccgtt gctgctgacc
     1981 gccggaaagc tggccgaaac gcttgcccac ctggtcggcc agtttgatat cgccgaggat
     2041 agccatcaag ccttcctggg taagatggac gccataaagg ccggcgtaca acgtaatgat
     2101 ctcgccgacg gtgagctcgg gttgaaagct ggtggactgc aactggacgc ccatcgaagc
     2161 gcgggcatag atgggctggt ggcggatatc gtatcccgcg acgaggatgg ttccggacga
     2221 cgctttgatc aggccttcgc aggcgctgag cgtgctcgtc ttgccagctc cgttggggcc
     2281 aaggagaccg aatatctcac ccggccggac ttcaaaggag acgttgcgga ccgcctggaa
     2341 cttgccgtaa aagatatcca ggttttccac ccgcaggacg ggtaggctgg ttgaagtgtt
     2401 catggccgct aaggtaggag gttaacggtg ggccgccaat agtagggcgg ccattttatt
     2461 cttacggtcg gtgagcgggg gaaagcgggc ggtaaacggc ggcgctggcg ggtatgcatc
     2521 aggataattg ggccgaaatc ggccccatta acgccggcta caggtaagat gggccgcggg
     2581 taaagatccg cgcctcggca ggccttcccc tcgggtgatt gggtatatag gcaaaaaaaa
     2641 gggggctgtg cgctgcacaa ccccggcttt tagaaggtat gatcgtaatt tagattcggg
     2701 gatcgaatta aagacccgtt accattgtgg tttccgggat aatgttattc acctggaact
     2761 cgcggacaac gtcgctgttg gccgtgcgaa caatgaaggt cgggttgata tcggcatccg
     2821 tgttcagaaa acggacattc ttcgagaagt gaacgtcgtc gaactgctgg ctgaaaacga
     2881 catcaccgtt atcgttcttg atgacgagcc agaacttctc gccggtcggg ttctcataat
     2941 ctaccttgaa aacgacgtca ttgttggtga cgccctggta agtcacagat acctgcgcgt
     3001 tggtggccgt tgctgccttc ctggcgccgc cggcatttgc aaagctggcc atggggctgc
     3061 cgatgagaaa cattactgcg gtcgagaata ctgcgatgat ccgcactgcg tggtttttct
     3121 tgttggtgtt catattattc gttttatgtt taagaacctt gttaatgatc agggatggcg
     3181 agcaaattgg cgagcaatgc gagtggcggg agcaactgtt agtggagatc tatgatctat
     3241 gatcaatgac aatacaaaaa tatgaccagt cagcggtgcc ggcgagctac atttggcggg
     3301 tccgcattat ggcaaattcc gtgaaatgtc ttttaaataa ttgataacca catttttcaa
     3361 ctgcaatacg aaatgataac caacattaaa attaactgtc aatgaaacgg ataattatta
     3421 ttctcttaac acttgcgccc atggctgcga cgctgcaggc gcaggataac cctttgtgga
     3481 tgcggtaccc cgccatctcg ccggacggtc aaacgatcct tttcagttac aacggagata
     3541 tctattcggt gccttccagg ggcggacagg ccatgccgtt gaccatcagt gagtcttacg
     3601 aattctcacc ggtatggtcg cacgacggaa agtggatcgc gtttgcctcc gaccggtacg
     3661 gcaattttga tgtgtttgtg atgccggcaa cgggcggcgt ggcgaagcgg ctgacctatt
     3721 tctcagggaa tgaggttccg agcagtttca ccggcgatga caagagcatc cttttctcat
     3781 ctctccggca ggacctggta agcaatgcac aattccccgg tcaaatgccg cagttgttca
     3841 gcgtaccggt gacaggcggc cgtgtgacgc aggttctggc ggtggcagcg cccgaggcaa
     3901 aggccaatgc cgcaggcgac aagctgatct ttgaggatca aaaaggatac gaaaacgagt
     3961 ggcggaaaca tcatacttcc gccattacgc gggacatctg gctctacgac ctgcgctccg
     4021 gtaaatttca acaactaacc agctttagag gcgaggaccg cagcccggtg atcggttcgg
     4081 acgaccggga cttttactat ttgagcgagc aggacgggac aatgaatgta tatcgtgaca
     4141 acctcgccgg cccgggcaag ccgctacaat tgacccattt tgcgcgcaat cccgtccggt
     4201 tcctgaccgt atcgaatgac aataccctgt gttacggcta tgacggcgag atctatacgc
     4261 agcggccgga cgggcagccg cagaaagtgg ctatccggat cgcggaggat ggccggaatg
     4321 aaatcgagaa agtattgccg gtaaatggcg gcgtaacaga aatgaaaatg agcccgaacg
     4381 gaaaagagat cgcattcgta ttccgcggtg agatctttgt cacgtctgtg gacgggggta
     4441 tcaccaaacg ggtcacgagc actccctggc aggagcggag tgtgggctgg agccccgacg
     4501 ggcggtccct gatctatgcc gcggagaagg accacaactg gaatatttat acgacgagca
     4561 tcagccggaa ggaggaacct tatttttatg cgagcaccgt attgaaggtg gagacagtgg
     4621 tggccacacc ggcggaagaa ttccagcctg ctttttcccc ggacggcaaa gaggtggcct
     4681 atctcgagga ccgtactacg ctaaaggtga tcaacctcgc gacgaaaaaa tcccgggtcg
     4741 tccttcctgc ggataagaac tattcgtatg cggatggcga ccaatattac caatggtctc
     4801 ccgacagcaa atggttcctc gtgcaattcg gcccgcctga acgggtaatg actccggagg
     4861 tagggttggt tgcggctgac ggcaaaacac ccgtcgtaaa tctcacgctc agcgggtatg
     4921 atgacataat gcccaaatgg tcgatggacg gaaagatgat gatctggggc tccgatcgcg
     4981 agggagccag gcagcaggca ggttatccgg tgacagcgga cgtttatggc atgttcttca
     5041 ccaaagatgc atttgaccgg ttcaaattat cgaaggaaga cctggcgttg gtaaaggagg
     5101 cggaggcaaa agaaaaggca gaagcggaca aaaagaaggc tggtaaggat acagccaacg
     5161 cgaaaaaaga t
//
LOCUS       DATGKE010000330         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1427070, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000330 DATGKE010000000
VERSION     DATGKE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427070"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VKU83_05700"
     CDS             complement(<1..651)
                     /locus_tag="VKU83_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLI93078.1"
                     /translation="MPRFTVDADISRAKTLDTAFYTDPELFLLAKEKLFIPSWQFIGD
                     ASLVASPCDAYPLTLLPGYLEEPLVLVCGKDEEIRLLSNVCTHRGNIVVNEACNLTQL
                     RCRYHGRVFDLDGGFRSMPEFREVCDFPSAADNLTRLPLESWGKWLFTSLGGGDDFRL
                     YWGDLMRRLHWLPLDNYELRPELSRDFTVKAHWALYCENYLEGFHIPFVHAGLNSVI"
     gene            697..1185
                     /locus_tag="VKU83_05705"
     CDS             697..1185
                     /locus_tag="VKU83_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLI93079.1"
                     /translation="MSEDLQIARGTKAEQYAALLPQIRALMEGERDGVANLANVAAAL
                     KEQFGWLWVGFYLIKDGELVVGPFQGPVACTRIRKGRGVCGTSWAEDRTLIVPDVESF
                     PGHIACSSLSRSEIVVPIRAGQTIIGVLDADSDKRNAFDEEDQRWLETLVAQLPGITD
                     VG"
     gene            1243..2031
                     /locus_tag="VKU83_05710"
     CDS             1243..2031
                     /locus_tag="VKU83_05710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLI93080.1"
                     /translation="MKKKLIALGSFLLFLTAPAVAQQGAAVPPPSVILAKKEVPILCY
                     HQIRDWKPTDSKVSKDYIMPPSDFRAEMKMLHDSGYHTILPDQLYAYLTKGAALPSKP
                     VMLTFDDNDEDQYTVAWPELKKYGFRGVFFIMTITMNKLPHYMSVAQLKELNGAGNVI
                     CSHTWDHHNVKKYQGKDWDIQIDKPTKQLEAITGAPIKYFAFPFGLWNHQALPELRKR
                     GFIAAFQLADKRDPTDPLMTIRRIIVGGGWSLKTFDAAIKRDFH"
     gene            complement(2028..>2485)
                     /locus_tag="VKU83_05715"
     CDS             complement(2028..>2485)
                     /locus_tag="VKU83_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HLI93081.1"
                     /translation="ETEPSFSFQIGDTLDNYSTASYDTAAVATLDVHGNQYGLLTASK
                     CFRLSDNLDCSSCHDPHANEYGKMAVYSQRCISCHNAASGHTCSSPAVKRMGLTGNCI
                     DCHMPALPSNAITLQVNGEAQLVHDLVRTHRVAIYADATRQYLEKIGKR"
BASE COUNT          608 a          726 c          659 g          492 t
ORIGIN      
        1 gatcaccgag ttcagcccgg catgtacgaa cggaatatga aaaccctcga gatagttctc
       61 gcaataaagc gcccaatgag cctttaccgt gaaatcgcgc gagagctcgg gtcgcagctc
      121 atagttgtcc aggggcagcc agtgcaatcg ccgcatcaga tcgccccaat aaagcctaaa
      181 gtcatctcca cccccaagcg aagtaaaaag ccatttcccc catgattcca gcggcagccg
      241 ggtgaggtta tccgcggccg acggaaaatc gcagacctca cggaactcgg gcatgctgcg
      301 aaagccgccg tcgagatcga agacccgtcc gtgataacgg caccgcagtt gcgtcaggtt
      361 gcaagcctca ttcactacaa tattccctct atgcgtacat acattgctca ataaccgaat
      421 ctcctcatcc ttgccgcaga ccagcaccag cggctcttcg agatagcccg gcaacagcgt
      481 caatggatat gcatcacagg gcgaagcaac caaagaagcg tcgccaataa actgccacga
      541 agggataaac agcttctcct tggccaataa aaatagctcg ggatcggtat aaaaggccgt
      601 gtcgagcgtt ttggcccggg aaatatcggc atctacagta aatctgggca tatcccaaga
      661 taggaaagat tgaggagaca tcgtaccttt gcgcccatgt cggaagatct acagatagcc
      721 cggggcacca aggccgaaca atacgcggcc ctgctgccgc agatcagggc cctcatggag
      781 ggcgagcggg atggcgtggc caatctggcc aatgtcgccg cggcgcttaa ggagcagttc
      841 ggctggttgt gggtggggtt ttatctgatc aaggacggtg agctggtggt cggccctttc
      901 cagggccctg tagcctgtac acgtattcgt aaaggcaggg gcgtttgcgg gaccagctgg
      961 gcggaagacc ggacgctcat cgtgccggac gtcgagtcct tccctggtca cattgcctgt
     1021 agcagcctgt ccagatcgga gatcgtggtc cccatccgcg ccggccaaac gattatcggc
     1081 gtgctcgacg cagacagcga taagcggaac gcattcgacg aagaagacca acgctggctc
     1141 gaaacgctgg tcgcacaact gccgggcatc accgacgtcg gttaagaacg gcctccggcc
     1201 gattccggca catgtaaaca tgtacaaatt ttaaaattca atatgaagaa gaaactgatt
     1261 gctctcggat ccttcctcct gtttttaacc gctccggcag tggcccagca aggtgccgcc
     1321 gttcctccgc cgtccgtgat cctcgcaaaa aaggaagtcc cgatcctctg ttaccatcag
     1381 atccgcgact ggaagccgac ggattccaag gtctccaaag attacattat gcccccgtca
     1441 gatttcaggg ccgaaatgaa gatgctgcac gacagcggct atcataccat tctgccggat
     1501 cagctctacg cctatctcac caaaggcgct gccctgccca gcaagccggt catgctgacc
     1561 ttcgacgata atgatgaaga ccaatatact gtcgcctggc cggaactgaa gaaatatggt
     1621 ttccgcggcg ttttcttcat catgaccatt accatgaaca agctgcccca ctatatgagc
     1681 gttgcccagc taaaggaact caatggcgcc ggcaacgtta tctgcagcca tacctgggac
     1741 caccataacg tcaaaaaata ccagggcaag gactgggata tccagatcga caagcctacc
     1801 aaacagctgg aagcgatcac cggcgcaccc atcaaatatt tcgccttccc attcgggttg
     1861 tggaaccacc aggcgctgcc cgagctgcga aaaagaggat ttatcgccgc tttccagctg
     1921 gcggataaaa gggatcctac cgatccgttg atgaccatcc ggcggatcat cgtcggcggc
     1981 ggctggagcc tgaagacctt cgacgcagct atcaaacgcg atttccacta acgtttcccg
     2041 atcttctcca ggtattgccg ggtggcatcc gcatagatgg ccacccggtg cgtgcggacg
     2101 agatcgtgta ccagctgcgc ttcgccgttg acctgcagcg tgatcgcgtt cgaaggcagc
     2161 gccggcatat gacaatcgat acaatttccc gtcaagccca tccgcttgac ggccggcgat
     2221 gaacaggtat gccccgaggc cgcattgtgg cagcttatac agcgctgcga gtaaacggcc
     2281 atcttgccat attcgttggc atgcggatcg tgacacgagg aacaatccag gttgtccgat
     2341 agccggaaac acttgctggc cgtcagcagc ccatactggt tgccatggac atccagcgtg
     2401 gcgacagcag ccgtgtcgta ggaggccgtc gaataattat ccagcgtatc ccctatctgg
     2461 aaactaaagg aaggctcagt ctcct
//
LOCUS       DATGKE010000331         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_935094, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000331 DATGKE010000000
VERSION     DATGKE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935094"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VKU83_05720"
     CDS             <1..756
                     /locus_tag="VKU83_05720"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLI93082.1"
                     /translation="VGSVTFTLGNVLVFVLILYVSHQFQKYIGFLFGVQDDRAMPQEG
                     KKGSRLVVFRLLLVIIGFFLAVVASGLPIDKITIVLGALGVGVGLGLQSIVNNLVSGI
                     ILIFERPFEVGDYIELNGKKGIVRDMGIRSSRLVTEDGTEIIMPNGDLLSGEVINWTV
                     QNNKVRIEVPITVEAGPTLDEINTIVQDTLKDHAGLSQENKPKVLLSAANEKTLSFTI
                     VAYVGNISQIQSIKSEILRLVHLRLKEKGIKTV"
     gene            768..1256
                     /locus_tag="VKU83_05725"
     CDS             768..1256
                     /locus_tag="VKU83_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLI93083.1"
                     /translation="MKSTGKSAGAVIRELEAALNGGHAHASFEDAVKKVDHKHLGETP
                     GGLPYSIWQLVEHIRISQWDILEFSRNPKHVSPDWPDGYWPKEKTPPHAADWERSLDR
                     IRADRKAFIELLHEAGDEIHTPFPHGDGQSLFREAVLIIDHTSYHTGEIIVLRRLLND
                     WK"
     gene            complement(1239..2012)
                     /locus_tag="VKU83_05730"
     CDS             complement(1239..2012)
                     /locus_tag="VKU83_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLI93084.1"
                     /translation="MSSPIFRRLRCGTSGLVLPVPNKKAFPEAFRERPRLSYYASLFN
                     SIEINSSFYKVPMPVTFARWAQEVPAGFEFTVKLWRGITHVAGLEFAPRDVSRFMDAV
                     HGLAEKKGCLLIQLPPALTAAAAGQLDRLLTCLHSSDPGRTWKPAVEFRHRSWDAARI
                     SAILERHYAARVLHDMPSSPTVELPSGHAAIPFVYLRFHGPEGRYKGRYAQPFLLAWA
                     ERISGWLAEGKDVYVYFNNTIGGDALADLAQLQALLPVV"
     gene            2104..3150
                     /locus_tag="VKU83_05735"
     CDS             2104..3150
                     /locus_tag="VKU83_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI93085.1"
                     /translation="MTPLNTAICSFGMSGRVFHAPFLDASPYFQLYSVWEREKNLAAQ
                     IYPGVKVRRTLEALLADDEVELVIVNTPNYTHYDYTKQALLAGKHVIVEKPFTVTEAE
                     GLELIALAKKTGKKLTSFQSRRFDSDYRTVKRIVDSGVLGDIVEAEFHYDRFTPTLSP
                     KVHKETAGPGRGLLYDLGSHLIDQALQLFGDPDEVFADFMVIREHSIVDDYTEVLLYY
                     PRMRVRLKSSYLVREPLPSYSLFGHKGTFLKSRTDVQERRLLAGEKPGGPDWGVEPET
                     ERGLLHTEKDGRVIREYVPTERGNYMDFFTGVYEAIRNGKPLPVTAEQGVRIIRVIEK
                     AYQSRDERRIVAFH"
     gene            3153..>3855
                     /locus_tag="VKU83_05740"
     CDS             3153..>3855
                     /locus_tag="VKU83_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93086.1"
                     /translation="MRAAILYILLLLPAIRPDAQETRKEGLRPLAFARELAATAMRIW
                     PDSGNLPPGASPSGKWELMLATMRLWYSTGDARYYTYARRQADRWLAREDGGREDPAR
                     EDPATFPTGGPGTVDDCAGRLLLRLFEVTEKPDYYRAAARLRQWFRRPPGTPRHVLVP
                     VVIGNPCCIAPFRAHYAALFHEDSVFDEIGREFVDIERYLYVGQTGALKKGTGATCWG
                     MGLVDALQYFPAANPG"
BASE COUNT          818 a         1066 c         1172 g          799 t
ORIGIN      
        1 gtcggcagcg ttacgtttac gctgggcaac gtgctggtat tcgtcctcat cctgtacgtt
       61 tcgcatcagt tccagaaata tatcggcttt ctttttgggg tgcaggatga ccgggcaatg
      121 ccgcaggagg gcaaaaaagg atcgaggctg gttgtcttcc gcctcctcct ggtcatcatt
      181 gggttcttcc tggcggtggt ggcttcgggc ttacccatcg acaagattac gatcgtcctt
      241 ggcgccctcg gcgtcggcgt cggccttggc ctgcagagta tcgtgaacaa ccttgtttcc
      301 ggcataatcc tgatctttga aaggccgttc gaggtggggg attatatcga gctgaatggc
      361 aaaaaaggca tcgtgcggga catgggaatc cgttcgagcc gactggtcac cgaggacggc
      421 acggagatca tcatgccgaa tggcgacttg ttgtccgggg aggtgatcaa ctggacggtg
      481 cagaataaca aagtccggat cgaagtcccg atcaccgtgg aggccggacc taccctcgat
      541 gaaatcaata cgatcgtcca ggacacgttg aaggaccatg caggcctgtc ccaggaaaac
      601 aaaccgaaag ttttattgtc cgcagccaac gagaaaacgc ttagttttac cattgttgcc
      661 tacgtgggga atatcagtca gatccagagt atcaagagtg agatcctccg tctggttcat
      721 ctccgcctga aagaaaaggg tattaaaacg gtataaaaac aatcattatg aaatcgactg
      781 gcaagtccgc gggagcggtg atccgcgagc tggaagccgc gctcaacggt ggccacgcac
      841 atgcttcgtt cgaagacgcc gtcaaaaaag ttgaccacaa acaccttggc gagacgccgg
      901 gaggccttcc ctacagtatc tggcagctcg tggaacacat ccggatcagc cagtgggata
      961 tcctcgagtt ttcccgcaac ccgaagcatg tcagtcccga ctggccggac ggatattggc
     1021 cgaaagaaaa gacgcccccg catgccgcgg attgggaacg ttcgctggac cggataaggg
     1081 cagaccggaa ggcgttcatt gaattattgc atgaggccgg cgacgagatc catacccctt
     1141 ttccgcatgg tgacggccag tcacttttcc gggaagcggt gctgatcatc gatcatacca
     1201 gttatcatac gggcgagatc atcgtcctga ggcggctgtt aaacgactgg aagtagcgct
     1261 tgcagttgcg ccaggtctgc gagggcatcg ccgccgatgg tattgttgaa atacacatac
     1321 acgtccttcc cttccgcgag ccacccggat atcctttcag cccatgccag caggaagggc
     1381 tgagcgtatc tgcccttgta ccgtccttcc gggccgtgaa accgcaaata gacgaaggga
     1441 atggcggcat ggccggaggg aagctccacg gtgggcgacg agggcatgtc atgcagcact
     1501 ctggccgcat agtgcctctc gagtatcgcg ctgatccggg ctgcatccca ggaccggtgc
     1561 cggaattcca cggcgggttt ccaggtccgg ccagggtcgc tgctgtgcag gcaggttagc
     1621 agccggtcca gttgtcctgc tgccgcggcc gtcaatgcgg ggggcagctg gatcagcagg
     1681 caaccttttt tttccgctaa accgtgaacg gcgtccatga aacggctgac atcccgcggc
     1741 gcaaattcca gccctgcgac atgggtgatt ccccgccata gttttacggt aaactcgaat
     1801 cccgccggta cctcctgcgc ccatcgggcg aacgtgaccg gcatggggac cttatagaag
     1861 ctgctgttga tctcgatact gttgaaaagc gaagcgtaat agctgagccg gggccgctcc
     1921 cggaacgctt ccgggaatgc tttcttgttc ggcacgggta ataccaggcc gcttgttccg
     1981 catcgaagtc tccggaaaat agggctgctc atgctgcagg ggaaagcaaa gttgcggcca
     2041 ggacatatat ttgtatgccg atttccgctc ccggcccgcg gaaaacagct taaattgagc
     2101 gatatgactc ctctcaacac ggcgatctgt tcctttggca tgtccggtcg cgtttttcac
     2161 gctccgttcc tggacgcaag cccttatttc cagttgtatt ccgtatggga gcgggaaaag
     2221 aaccttgccg ctcagatcta tccgggggtc aaggtacgca ggacactgga agcgttgctg
     2281 gcggatgatg aagtggaact ggtgatcgtc aacaccccga actataccca ctacgattac
     2341 acgaagcagg ccctgcttgc ggggaagcat gtcatcgtgg agaagccatt caccgtgacg
     2401 gaagcggagg gcctggagct gattgcactg gccaaaaaaa cggggaagaa gcttacctcc
     2461 tttcagagcc gccggttcga cagcgattac cgcacggtga agcgcatcgt ggactcgggc
     2521 gtgctgggag acatcgtcga ggcggagttc cattacgacc ggtttactcc gaccctcagc
     2581 ccgaaggtcc ataaggaaac ggcgggcccg ggccgcggcc tcctctatga ccttggttcc
     2641 catctcatcg atcaggccct gcagttgttc ggcgatcctg acgaggtgtt cgcagacttc
     2701 atggtcatcc gcgagcattc gatcgtggac gactatacgg aagtgttgct ctattatccc
     2761 cggatgcgcg tccggttaaa gtcgagttac ctggtccgtg agcccctccc ttcctatagt
     2821 ttgtttggcc ataagggaac ctttttgaaa tcgaggaccg acgtgcagga acgccggctc
     2881 ctggccggtg agaagcctgg gggtccggac tggggtgtcg agccggagac ggagcggggg
     2941 ctgttgcata ccgaaaagga tggaagggtc atccgcgagt atgtccctac ggaaaggggt
     3001 aactatatgg acttttttac gggcgtctat gaagcgatcc ggaacggcaa acctttgccg
     3061 gtgacagcgg agcagggcgt acggatcatc agggtaatag aaaaagctta tcaaagccgc
     3121 gacgaacgcc ggatcgtggc ttttcattga ctatgcgcgc ggccatactc tatatcctat
     3181 tactccttcc ggcgattcgg cccgatgccc aggaaacccg gaaggagggg ttgcggccgc
     3241 tcgccttcgc cagggagctg gcggccacgg caatgcggat ctggccggac agcgggaacc
     3301 ttccgcccgg agcgtcgccc tccgggaaat gggagctaat gctggccacg atgcggcttt
     3361 ggtacagcac gggggatgcc cggtattaca cttatgccag gagacaggcc gaccgctggc
     3421 tggccaggga agacggcggc agggaagacc cggccaggga agacccggct accttcccga
     3481 ccggcggccc gggcaccgtt gacgactgcg ccggcagact attgctgcgg ctctttgaag
     3541 tgactgaaaa gcccgattat taccgggcgg cagcgcgcct gcgccagtgg ttccggcgtc
     3601 cgcccgggac tccccgccac gtgctggttc cggtcgtaat aggtaacccc tgttgcatcg
     3661 ccccattccg ggcgcactat gccgcgcttt tccacgaaga cagcgttttt gacgagatcg
     3721 ggcgcgagtt tgtggatatc gagcgatacc tgtatgtcgg gcagacggga gccctgaaaa
     3781 aggggaccgg agcgacttgc tgggggatgg ggctggtaga cgctctccag tattttcccg
     3841 cggccaatcc tggac
//
LOCUS       DATGKE010000332         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1251632, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000332 DATGKE010000000
VERSION     DATGKE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251632"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1269
                     /gene="nagA"
                     /locus_tag="VKU83_05745"
     CDS             160..1269
                     /gene="nagA"
                     /locus_tag="VKU83_05745"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HLI93087.1"
                     /translation="MSTALIAREIFTGKDIQTGKAVLIRDGKIVDLVAATEIPAGYRV
                     RDLPGYMLAPAFIDLQIYGGNGLLFSADLSTAALEATYEYCLQGGCTRFMITMATNSI
                     EKFIRGLEVAREYRAGGGKGLLGVHLEGPYINPAKKGAHLEKFIKTPTVEEIGLLLQK
                     GKDVFRMMTLAPERCDRACVELLLRNGIIVSAGHSNATYEEAVDGFYQGIPAATHLFN
                     AMSPLQGRQPGMVGAVYDNNDVRCSVVCDGIHVDFASVRISKKIMQDRLFFITDAVTE
                     VQYGEYSHIFTGDRYTLPDGTLSGSSLTMMKAVTNAVEKAGIPLPEALRMASVYPAAV
                     LGEQGKLGCIAPGACADLVLLDEQLNILQVMVDGE"
     gene            1389..1805
                     /locus_tag="VKU83_05750"
     CDS             1389..1805
                     /locus_tag="VKU83_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93088.1"
                     /translation="MENAVFSKNYPLTILVAENGQDATSSAKSLLIQLGYQPEVATSS
                     QEMLAMTRKKTYDVVMADVQIPDLDRILASQTGGSSRRPIFIGMTVSDSADTGGGCLR
                     PGMDHSICKPVDPAELSLQLQACSVLSGTRRIRGEK"
     gene            complement(1833..>2210)
                     /locus_tag="VKU83_05755"
     CDS             complement(1833..>2210)
                     /locus_tag="VKU83_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLI93089.1"
                     /translation="APIARDHFDGHIYILTGGYSFSATCLFAGALKGQSNVTLVGEET
                     GGGYYGNTAWMIPDVTLPATGVRFRLPRYRLVVDRRRVKDGRGVMPDVPALPSVEALT
                     KGVDFKTAKVRELIRLKSAESGN"
BASE COUNT          488 a          660 c          616 g          446 t
ORIGIN      
        1 ggtcatcgat cctctcggcg agacgcttta tcacgccggc catgaggaag ggatccatac
       61 gctgaccctc gtcagggagc agctcgaaac cattcgcagt cgcttccctt tctggcgcga
      121 cgccgaccat ttttatattg aaccctaaaa aaccagcaca tgtcgacagc attgatcgcg
      181 cgcgagatct tcaccgggaa ggatattcaa accggcaagg ccgtcctcat ccgcgacggc
      241 aaaatcgtcg accttgtggc cgcgaccgaa attcccgccg gttaccgggt ccgggatctc
      301 cccggttata tgctcgcgcc tgccttcatt gacctccaga tctatggcgg taatggcctt
      361 cttttttccg cggacctgtc caccgccgct ttggaagcca cgtacgaata ttgcctgcag
      421 ggcggctgca cccgcttcat gatcacgatg gccaccaatt cgatcgaaaa atttattcgc
      481 ggcctggagg ttgcccgcga atatcgcgcc ggcggcggaa aaggcttgct cggggtgcat
      541 ctcgaaggcc cctatatcaa cccggcgaag aaaggagcac acctggaaaa gttcatcaag
      601 actcctaccg tcgaagagat cggcctgctc ctgcaaaaag gaaaggacgt attcaggatg
      661 atgactttgg cccccgaacg ctgcgaccgc gcctgcgttg agctgctcct gcgcaacggc
      721 atcatcgtgt cggcgggcca cagcaacgcc acgtatgagg aagcggtgga cgggttctat
      781 cagggtatcc cggcagcgac ccatctgttc aatgccatgt ctccgctgca ggggcgtcag
      841 cccggcatgg tgggagctgt ctacgacaat aacgacgtcc gctgcagcgt tgtatgtgat
      901 ggcattcacg tggactttgc atcggtgcgc atcagcaaaa aaatcatgca ggaccgcctt
      961 ttcttcatta cggacgccgt gaccgaggtg caatacggag aatacagcca tatattcacg
     1021 ggcgatcgct atacgctccc ggatgggacc ctctccggtt cttccctaac catgatgaaa
     1081 gccgtcacca acgcagtcga aaaagcgggt attccgctac cggaggcgct tcgaatggct
     1141 tcggtttacc cggcggccgt cctgggtgaa cagggcaaac tgggttgcat cgccccgggg
     1201 gcatgcgccg acctggtgct cctcgatgaa cagctgaata tcctccaggt gatggtggat
     1261 ggggagtaaa aagccccgaa acctagggta tccatgtaaa gattcgatcg cttcctcctt
     1321 atccggtcga aaaaggcccg taaggatttc atccttcggg gaccccggta ttaatatagc
     1381 gtattcttat ggaaaacgca gtcttttcaa aaaactaccc cctcaccatc ctggtggcag
     1441 aaaatggcca ggacgctacc agctcggcga aaagcctcct cattcagctg ggctaccagc
     1501 ctgaggtcgc gacatccagc caggaaatgc ttgcgatgac gcgcaaaaag acctatgatg
     1561 tggtcatggc ggacgtccag atcccggacc tcgacaggat actggcttcc cagaccggcg
     1621 gcagcagccg gcggccgata ttcatcggaa tgaccgtaag cgattcagcg gacaccggcg
     1681 ggggttgcct gaggccgggc atggatcact cgatctgcaa accggtcgat cccgccgaac
     1741 tgagcctcca gcttcaggca tgttccgtcc tgtccggcac tcgccggata cggggagaaa
     1801 aatgagtggc ctgaaaggca acgatcgatc cgtcagttac cgctttccgc cgactttaac
     1861 cttatcaact cgcggacttt ggccgttttg aagtcgactc ccttggtgag cgcctccacg
     1921 ctgggcagcg ccggcacatc gggcatgacg cccctgccat ccttcacgcg gcgccggtcg
     1981 acgaccagcc ggtacctggg aaggcggaaa cggacaccgg tggcgggaag tgtcacatcg
     2041 gggatcatcc aggccgtgtt tccgtaatag ccgccgcccg tctcttcccc gacaagggta
     2101 acattagact gacctttcaa ggctcccgca aacaggcagg ttgcggaaaa cgagtatcct
     2161 cccgtgagga tatatatgtg gccgtcaaaa tgatccctgg caatgggtgc
//
LOCUS       DATGKE010000333         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1266096, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000333 DATGKE010000000
VERSION     DATGKE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266096"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VKU83_05760"
     CDS             complement(<1..1093)
                     /locus_tag="VKU83_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93090.1"
                     /translation="MKKIYLLTAGFCVSMTLFAQSPGGVSAGLSLWLKADAASTLHLT
                     GSNVNSWDYVNAAKSFSFTGGTNAVVVNSAINGLPAVFFNGNTEMDGPTGANAPLPAG
                     SVAYSVFAVWSSAITGTSGGPYQRVWTQWGNNGIDGNGTSVWLYNGEWGDQPEISVGG
                     FITGEGNPYSTNTYYITEMNLLNQASNDLELTDNTDFATGPNVLSTEAGSGNTVRANL
                     STNINSLGYRVGSPAGEFFDGNLSELIVYTNPVSAGASKNEIFSYLSMKYGIPIGTNL
                     VSSSNTTVWDATAHAAYNNDVFGLAYDLGSGLNIQSSNPSTTAGVSGAGNIVLTPSDV
                     YFMDQLFMMVGHDNGSLSTEESTNMPAYAS"
     gene            1428..>2556
                     /locus_tag="VKU83_05765"
     CDS             1428..>2556
                     /locus_tag="VKU83_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93091.1"
                     /translation="MDFIYLLRVLMKKKWIIIGAALTASLIAFYFTRNQPRKFKSFAQ
                     ISTGFTQTEEIRMNPIEDNAGYNNEDIKFNNVTTTLMSRTVLDLLSYCMILHDLQSPN
                     PFEYPSQKDMQSQTYRSIDKVEAIRVFSRDLELMIPLNTYKAGDRRLLELLEMYGYGY
                     KTLLKELTAYRLPRTDYIQLEFETNNPLLSAFAVNNWYDQFLRYYRTVRSNRSIESID
                     TLQSLLDRKKQELDAKNQQLHDQGIINVGQENGAKWELILNKQSTLTDIQAHQTEDYY
                     ALQQVNQQLVALGVPTTPGKPNAAAATNDELLKLRNEMNDAYSQYINSGESDENLRKK
                     YEDLKAQYNQKVASAISNSSTPATADGTDEKSRLLDKKSALE"
BASE COUNT          665 a          684 c          655 g          552 t
ORIGIN      
        1 ccgaggcata tgcgggcata ttggtacttt cttcagttga cagtgagcca ttgtcatgac
       61 ccaccatcat gaataactga tccatgaagt aaacgtcgct gggcgtgagg acaatatttc
      121 ccgcaccgct cacgcctgcc gtcgtggacg ggttggacga ctgaatattc aaacctgagc
      181 cgagatcgta cgccaatcca aagacatcgt tgttataggc ggcatgcgcg gttgcatccc
      241 agaccgtggt gttcgaggaa gacaccaggt tggttccaat gggaatgccg tacttcatag
      301 agagatagga aaagatctca ttcttggaag ccccggcact gaccggattg gtatagacga
      361 tcagctcgga gagattgccg tcgaagaact cccccgccgg gctgcctacc cggtagccga
      421 gcgaattgat gttcgtcgaa aggttggccc tgacagtgtt cccgctgccg gcctccgtgc
      481 tcaatacatt tggcccggtt gcaaaatcag tattgtccgt cagctcgaga tcattgctgg
      541 cctgattcag caggttcatc tcggtgatat agtaggtgtt ggtcgaataa ggattccctt
      601 ccccggtgat aaacccgccc accgagatct ccggctggtc gccccattcc ccgttgtaca
      661 gccagacgct tgtcccgttc ccgtcgatcc cattgttgcc ccattgggtc caaacccgct
      721 ggtaggggcc gccggacgtg cccgtaatgg ccgaactcca aacggcgaat accgaatacg
      781 cgacgcttcc cgcgggcagc ggagcgtttg cgcccgtagg gccgtccatc tcggtgttgc
      841 cgttgaaaaa caccgccggc aggccattga tcgccgaatt gacgacgacc gcattggtcc
      901 cgccggtaaa gctgaaggac ttggccgcgt ttacataatc ccaggaattg acattggaac
      961 cggtaagatg caaggtactt gcggcgtcag ctttaagcca tagggacaga ccggcgctga
     1021 caccgcccgg ggattgcgca aacagagtca ttgaaacaca aaacccggcg gttaacaggt
     1081 aaattttttt catacgcatg attaataaag gatagataat ggcctccagg ggcaggacga
     1141 tggcggccgt tcaaggatgg acttttatcg aagaaatgat ctttgcgggg gatttttgga
     1201 tcgaaaaact cggctctaaa gatactcttt ttgataatcg ggcaaaaaat aataacaaac
     1261 accctaattc ggccaaatac ttgcatgaat aaccggagct tattatattt acacccgtgt
     1321 tattaccccc ctgggggaga tatccgtctc ccttcatagt gcaataatat ctaatatatt
     1381 acctatttta agccattgta actcgtattc ttaactaaaa ggacccaatg gattttattt
     1441 atttgttgag ggtgttgatg aagaagaagt ggatcattat tggggcagcc ctgaccgcat
     1501 cgctgatcgc attttacttt accaggaacc agccaaggaa gtttaagtca tttgcccaga
     1561 tctccaccgg cttcacgcaa accgaggaga tccggatgaa ccccatcgag gacaacgccg
     1621 gttacaataa tgaggatatc aagttcaaca atgtcacgac tacgctgatg tcccggacgg
     1681 tcctcgatct gctgtcctat tgcatgatcc tccatgacct gcaaagcccc aaccccttcg
     1741 agtatccgag ccagaaggac atgcagtcgc agacctaccg gtcgatcgac aaggtggagg
     1801 ctatccgggt attttcccgc gaccttgaac tgatgatccc gctgaatact tacaaggcgg
     1861 gcgatcgccg gctgcttgaa ctgctggaaa tgtacgggta tggttacaag accttgctta
     1921 aggagctgac cgcgtatcgc ctgccgcgaa ccgactatat tcagcttgag ttcgagacca
     1981 acaatccttt gctctccgcc ttcgccgtga ataactggta cgaccagttc ctccggtatt
     2041 accggaccgt tcggagcaac cggtcgatcg aatccataga taccctgcaa agcctgctgg
     2101 acaggaaaaa acaggagctg gatgcgaaga accaacagct gcacgatcag ggcatcatta
     2161 atgtcggcca ggagaacggc gccaaatggg agttgatcct caacaagcag tcgacactca
     2221 ccgacattca ggcgcatcag acggaagatt attatgccct gcagcaggtc aaccagcaac
     2281 tggttgccct gggcgtgccc acgacgccgg gtaaaccgaa cgccgccgct gcgaccaacg
     2341 atgagttgct gaaattgcgg aacgaaatga atgatgccta ctcgcaatac atcaacagcg
     2401 gcgaatcgga cgaaaacctc cgcaagaaat acgaggacct gaaggcgcaa tacaaccaga
     2461 aagtagccag cgccataagt aacagcagca ctccggccac cgccgacgga acggatgaaa
     2521 aaagcaggct ccttgacaaa aaaagcgcgc tggaag
//
LOCUS       DATGKE010000334         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1326655, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000334 DATGKE010000000
VERSION     DATGKE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326655"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VKU83_05770"
     CDS             <1..487
                     /locus_tag="VKU83_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93092.1"
                     /translation="KLDWETAAETNSNYFTILRSRDATQWNDVLVVPAHGNSNAPSYY
                     QAYDNDPYPGVSYYRLAQTDDNGNVNYSEVCVVNVAGETKGVAVYPNPADDHVVVVPS
                     TSGSLTVQIVDARGMAIGQPIQSTGSNVVVSVRGLAPGVYFLRTLQGARAQITPIVIR
                     R"
     gene            complement(501..2573)
                     /locus_tag="VKU83_05775"
     CDS             complement(501..2573)
                     /locus_tag="VKU83_05775"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HLI93093.1"
                     /translation="MRYLYISLLFFFTQDCIAQDTATIIHKEDSLKLVYGGASIFSGK
                     IGTAGIPYVLRRQEEVIKGCTWQLITIRARDLRPFRLDGRITGSDDAIACESDPPEDR
                     IKVVRQVVGRSHSLLNNAVYERRNDWLFSVDNYYSKVKLTPAEDSGFDISVSGWEIVL
                     RFRPRYYREHRGLSRFDPSAYAVWKKPVVGWCSWFAYLDKVNEQEIKATADVISEQLR
                     PFGLQYLQIDDGYEQQPIGLPDTWLHANDKFPSGLRQLASYIAAKGLIPGIWTNVTFA
                     DSAAAYDNRNLFVQDEQCRPASGNWIGYVMDGSNPGTIRRLVTPVYKGLNDDGFRYFK
                     LDALRHLKYEGYNSFKEYFQQRGVDRDEAYRNVAKEVRDQAGRDKFLLACWGIRPELV
                     GIADGCRVGSDGFSYAGLAQFNSYNNIIWRNDPDHIVLSAKEAYRSCTATSLTGSLFM
                     LTDKAEKYGHSPLVEAARRTIPVLFTQPGQVYDVDPSRSALIAGADVEMSGSGPRPFD
                     ASSNTTTGLFELEINKPFGDWIVLGRLDDRDKILPFRDLGLDEKKEYLVFEFWTKNFR
                     GAFTARFIPGAIDTAFNCQAFCIREKLPHPQLLATNRHISCGGLEIRALAWADNTLEG
                     KSELVANDKYIIYVYEGPGYTFDKVTAKGAELISAEKTGFVRKITLLSTTGGETEWTV
                     RYKRTGKR"
BASE COUNT          562 a          753 c          718 g          599 t
ORIGIN      
        1 caagcttgac tgggaaaccg cagccgaaac caacagcaat tattttacga tactccgttc
       61 gcgtgacgcg acacagtgga atgacgtatt ggtagtgccg gcgcatggca acagtaacgc
      121 gccttcttat tatcaagcgt atgacaacga tccatacccg ggcgtatctt actatcggct
      181 ggcccagacc gatgacaatg ggaacgtcaa ttactcggaa gtatgcgtcg taaacgtcgc
      241 tggcgagaca aagggcgttg cggtttatcc gaaccctgca gacgatcatg tggtggtggt
      301 gccttccaca agcggcagtc tgacggtcca gatcgtcgat gcgcgcggaa tggccatcgg
      361 ccagccgatc cagtcgacgg gaagcaatgt ggttgtttcg gttcgcggcc tggcgccggg
      421 tgtctatttc ctgcggacac tgcagggagc acgggcccag atcaccccta tcgtgataag
      481 aagatagggt gtcacgggga tcaccgcttc ccggtcctct tgtaccggac ggtccattcg
      541 gtttcgcccc ctgtcgtcga aaggagcgtt attttccgaa cgaaaccggt cttttcggca
      601 gagatcagtt cagctccctt cgcggtcacc ttgtcgaagg tatagcccgg cccttcgtac
      661 acataaatga tatacttgtc gttcgcgacg agttcacttt ttccctctaa ggtattgtct
      721 gcccaggcca aggcgcggat ctccaggccc ccgcaggaga tatgccggtt tgttgccagg
      781 agttgcggat gcggcaattt ttcgcggata cagaatgcct ggcagttgaa agccgtatcg
      841 atggcgccgg gtataaaccg ggccgtgaag gcgccccgaa agtttttggt ccagaattca
      901 aaaaccaggt actccttttt ctcatcgaga ccaagatccc ggaatggcag gatcttgtcc
      961 ctgtcgtcca ggcggccaag cactatccag tccccgaagg gcttgttgat ttcgagttca
     1021 aaaaggcctg tggtcgtgtt actgctcgca tcgaacggcc gggggccgct gccgctcatt
     1081 tccacatccg cccccgcgat cagcgcggaa cgcgacggat cgacgtcata tacctggcca
     1141 ggttgcgtga ataagacggg gatcgtgcgg cgtgcggcct ctaccagtgg cgaatgaccg
     1201 tatttttccg ccttgtcggt cagcatgaag agcgagccgg taagtgaggt ggcggtacag
     1261 ctgcgatacg cttccttcgc gctcaaaaca atatggtcgg ggtcattgcg ccagatgata
     1321 ttgttgtaag agttgaactg cgccaggccg gcatagctga aaccgtcgct tccgaccctg
     1381 cagccatcgg cgattccaac cagttccggc ctgattcccc agcacgccag caggaatttg
     1441 tccctgcccg cctggtcgcg cacttccttt gccacattgc gatacgcctc atcccggtca
     1501 acacctcttt gctgaaaata ttccttgaag gaattgtagc cctcatattt caaatgccgg
     1561 agggcgtcca gcttgaagta acgaaatcca tcgtcattca atcccttgta gaccggggta
     1621 accagccggc ggatcgtgcc ggggttgctg ccgtccatga cgtaccctat ccagtttccg
     1681 gaggccggcc ggcactgctc atcctgcaca aacaggttcc ggttgtcata agccgctgcg
     1741 gagtcggcaa aggttacatt ggtccaaata ccgggaataa gccccttcgc ggcgatataa
     1801 gatgcgagct gccttaaacc cgaaggaaat ttatcgttcg catgcagcca ggtatcgggc
     1861 aggccgatgg gctgctgttc gtatccgtcg tcgatctgga ggtactgcag tccgaagggc
     1921 cgcagctgct cgctgatcac atcggccgtc gctttgattt cctgctcatt caccttgtcc
     1981 aaataggcga accagctgca ccagccgacc acgggcttct tccaaaccgc atatgccgat
     2041 ggatcgaagc gcgaaagacc gcgatgctcc cggtagtacc ggggacggaa acggagaacg
     2101 atctcccagc cgctgacgct tatatcaaag ccgctatcct ctgccggggt cagcttcact
     2161 tttgaatagt agttatcgac ggagaacagc caatcattcc tgcgttcata cacggcattg
     2221 ttgagcaggc tatggctccg gcccacgacc tggcggacaa ctttgatccg gtcttccggc
     2281 ggatcgcttt cgcaggcgat cgcatcgtcg ctgccggtga tccggccatc gaggcggaat
     2341 ggcctcagat cccgggctct tatggtgatc aactgccagg tacaaccctt gataacctct
     2401 tcctgccgcc gaagcacata cggaatgccc gcggttccga tcttgcccga aaatatgctg
     2461 gcgccgccat acaccagttt cagggaatcc tccttatgga tgatggttgc cgtgtcctgc
     2521 gcaatacaat cttgggtaaa aaagaaaagc aggcttatat acaggtatct cattcttgaa
     2581 aataacggca tcatttgttc ttcgccgcca gctgcatcgc attataggcg tt
//
LOCUS       DATGKE010000335         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_132533, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000335 DATGKE010000000
VERSION     DATGKE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132533"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VKU83_05780"
     CDS             complement(<1..213)
                     /locus_tag="VKU83_05780"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HLI93094.1"
                     /translation="MLSSIESAIEAIKQGKLVIVVDDEDRENEGDFITAARNVTPEII
                     NFMSKEGRGLICAPLVEERCDELGLEL"
     gene            572..>2166
                     /locus_tag="VKU83_05785"
     CDS             572..>2166
                     /locus_tag="VKU83_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI93095.1"
                     /translation="MIKRLLLSAITILSMLYSFGQETTSQMLGNVSSGGTGLAGATVT
                     ALHGPTGTKYVTTTRKDGRYNLDGLRVGGPYTLTVSYVGFKEEKQENIFLTIGQDYTG
                     DFTMTSDTRELSAVTVAAARQNRIFNNSHTGAQEIIGRAQLEQLPTVSRSIQDFTKLE
                     PTSNGLSFAGANPGMNNITVDGADFNNSFGLASTLGGQANAQPIALDALDQIQVQVSP
                     YDVRQGNFTGALVNSVTRSGTNQFRGTIYTYLKGPGTIGYKADDYTAPKTPFTYNVSG
                     ASLGGAIIQDKLFFFVNAEQSLQTAPATGFIASDATHPVTPGSVSIANADTLTALANL
                     LKSGTFKDAGGNTVKYDPGAFQGYSFKTNSYKVNARIDWNVNPANVLTLKYNYLKSYA
                     DQFPSTSRAVGTGLVGGQQPGNNAMPYYGAGYRIDNDLSIFILELNTRFGNTASNKLQ
                     IGYTRERDFRSAQTSDANFPLVDILYNGNGYTTFGYEPFTYNNQLNMDSYQLMETFTA
                     YKGAHEITIGTQDTYKKYQNAFAP"
BASE COUNT          516 a          702 c          486 g          462 t
ORIGIN      
        1 cagctccagc cccagctcgt cgcagcgttc ctccaccagg ggggcacaga tcaacccccg
       61 cccttccttg ctcatgaaat tgatgatctc cggggtcaca ttccgggccg ccgtgatgaa
      121 atctccttca ttttcacggt cttcgtcgtc aaccacaatt accagtttcc cttgttttat
      181 cgcttcaatt gcgctctcga tactggatag cattcaaaat cggtttaata tgatgcgggc
      241 catcggcccg ccattgacaa aattaacaat aaaaacgggg gaatgttagc gggagatgcc
      301 cctcgccaaa atataggcca ccgcccaccc cggcggccct tttcagccaa ttttgttaat
      361 aactttgtat attaacaaaa tcttatcatg cagccttttt ctacttttgc ttttctttag
      421 cacttcattg tcagctccat tttcctggaa aacccaccgc tagcgcctgc catttacttg
      481 ttaatgattt cttaatagcc caatggatac agtcatacaa aagctgtatt tctttgcagc
      541 cgcaaaatta accgcgcaaa aaccacttta tatgataaaa cgactgctat tatcggcaat
      601 cacgatcctc tccatgctgt attccttcgg gcaggagacg acctcccaaa tgctgggaaa
      661 tgtctccagc ggaggtaccg gcctcgccgg cgcgaccgtg acggccctgc acggtcccac
      721 gggtacaaaa tacgtcacca ctacccggaa ggacggtcgc tacaacctgg acggtctccg
      781 cgtcggcggg ccgtataccc tcaccgtaag ctatgtagga ttcaaagaag aaaaacagga
      841 gaacattttc ctgaccatcg gccaggatta caccggcgat tttaccatga cgtcggatac
      901 cagggagctc tctgccgtca ccgtggccgc cgctcgtcag aacaggatct ttaacaactc
      961 ccacaccggc gcccaggaga tcatcggccg ggcccagctg gagcagttgc ccaccgtcag
     1021 ccggtccatc caggacttta ccaagctcga gccgacctcc aacggtctga gcttcgccgg
     1081 agccaacccc ggaatgaaca acatcaccgt ggacggcgcc gacttcaaca actcgtttgg
     1141 tctcgccagc accctgggcg gccaggcgaa cgcccagccc atcgccctcg atgcgcttga
     1201 ccagatccag gtccaggtgt ctccctatga cgtccgtcag ggcaacttca ccggcgctct
     1261 ggtcaactcc gttacccgca gcggtaccaa ccaattccgc ggtacgatct acacctatct
     1321 gaaaggcccc ggtaccatcg gttacaaggc cgacgattat accgcgccga aaacgccgtt
     1381 tacctacaat gtatccggcg cttccctggg cggggccatc atccaggaca agctattctt
     1441 cttcgtcaac gccgaacaga gcctgcaaac cgccccggct accggcttta tcgcttccga
     1501 tgccacccac ccggtgactc ccggcagcgt ttccatcgcc aacgccgata cgctgaccgc
     1561 gctggccaac ctgcttaaaa gcggtacatt caaagacgcc ggcggcaata ccgtgaaata
     1621 cgacccgggt gctttccagg gctattcctt caagaccaac agctataagg tgaacgcccg
     1681 gatcgactgg aacgtcaacc cggccaacgt cctgaccctg aaatacaatt acctgaaatc
     1741 ctatgccgac cagttcccca gcaccagccg cgccgtaggt accggtctcg tcggcggcca
     1801 gcagcccggt aacaatgcga tgccctatta cggggccggc tatcgcatcg acaacgacct
     1861 gagtatcttt atccttgagc tcaacacgcg tttcggcaac acggcctcta acaagctgca
     1921 gatcggttat acccgggaac gggatttccg ctctgcccaa acctccgacg ccaacttccc
     1981 cctggtggac atcctttaca atgggaatgg ttatacgacc ttcggctacg aaccttttac
     2041 ctataacaac cagctgaaca tggattccta ccagctgatg gagaccttta ccgcgtacaa
     2101 aggcgctcac gagatcacga taggtacaca ggacacgtat aaaaaatacc agaacgcctt
     2161 cgcccc
//
LOCUS       DATGKE010000336         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_509722, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000336 DATGKE010000000
VERSION     DATGKE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509722"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1407)
                     /locus_tag="VKU83_05790"
     CDS             complement(22..1407)
                     /locus_tag="VKU83_05790"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HLI93096.1"
                     /translation="MNSRYTSLFRFSFACADLIVLNLVQLLLLNSLRRIPGNSGPAYL
                     LLFLVANILWLISAYATGVYIEEGNPNYMRFIKRTVKCLISFFLLMVLFIFFYHYPYS
                     RMFIVFSFIGFALMLVAMRLFMMRASLYANKSGKTGKRVVIIGYNEMAVKLAEWFSKQ
                     RNKSNVEGYFEDKEHVTELSLLPIIGNIDECLQYAMNNDISEIYSTISPERNPSIYEM
                     AYRAEKSLIRFKFVPDFKLYVNRNTHMEYLDQFPVLSLRPEPLEDVTNSIKKRIFDMV
                     FSALVIVFILSWMVPVLAILIKLSSRGPVFFSQWRSGKDNKNFQCYKFRTLSVNADAN
                     LKQVTMNDSRITPLGRFLRKTNLDELPQFFNVFKGDMSVIGPRPHMLLHTDTFSKITE
                     EYMVRHFVKPGVSGWAQIQGYRGEIKEEAQLRKRIEHDIWYLENWSLWLDIRIMLQTI
                     YLSIKGDKNAY"
     gene            complement(1813..>2744)
                     /locus_tag="VKU83_05795"
     CDS             complement(1813..>2744)
                     /locus_tag="VKU83_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93097.1"
                     /translation="LLFYPYFFLNSFVFYPVNYGLFFALLGLLALDRERKKNGYGKEG
                     KGYWRDLLAGVCLSLAVLCQQFYLVVPAAVCFARIILIMYKNRRPFLPALVQTTVGNI
                     ALLLPLIAPALVFIRWKGLTHPNFHVHSVAFIPSTVTAILFVIGFYFIPYLIQSYKSI
                     TGRNVCLALAGAILLVILFKPVFSDFQGAGLFTGITYHLMMLSGKISPLITTVSMVGL
                     TCCGILVFIVLCRSLSSSWDYTLFIICIFLAAAYAVNTEIGERHLLALIILLFLLILP
                     RIRTPVAAWYPWAMAVLGMGYFFYWTFFKYGAV"
BASE COUNT          789 a          639 c          688 g          628 t
ORIGIN      
        1 gctattttag gcatagagcg cttaataagc atttttgtcg cccttaatcg acaggtatat
       61 ggtctggagc atgattcgga tgtcgagcca taagctccag ttttccaggt accagatatc
      121 gtgctcgatc cgcttgcgga gctgcgcctc ctccttgatc tcacctctgt aaccctggat
      181 ttgcgcccat ccactgacgc cgggctttac gaaatggcgg accatatatt cctccgtgat
      241 cttggaaaat gtgtcggtat gcagcagcat atgcggcctg gggccaatta ccgacatatc
      301 ccctttgaaa acattaaaga attgggggag ctcgtccagg ttggtcttgc gaagaaacct
      361 gccaagcggg gtgattcggc tgtcgttcat ggtcacttgc tttaaattgg catccgcatt
      421 caccgacaag gtcctgaatt tatagcattg gaaatttttg ttgtctttgc ccgaacgcca
      481 ttgagaaaag aataccggtc cccggctgct gagcttgata aggatggcca acaccgggac
      541 catccaggac aggatgaata cgatcaccag ggcactgaac accatgtcaa aaatccgctt
      601 ttttatactg ttggtgacgt cctcgagcgg ttccggccta agggagagta caggaaactg
      661 atcaaggtat tccatatggg tgttccggtt gacatatagc ttgaaatcag gcacgaattt
      721 gaaacgaatc agcgacttct ccgcccgata cgccatttcg tagatgctgg ggttgcgctc
      781 cggcgaaatg gtggaataaa tctcactgat atcgttgttc atcgcatatt gaaggcattc
      841 atcgatattg ccgattatcg gcagaaggga aagctcggtg acatgctctt tatcctcgaa
      901 atatccttcc acgttggatt tattgcgctg cttcgagaac cattccgcca gctttacagc
      961 catctcgtta taaccgatga tcaccaccct cttacccgtc ttaccactct tattggcata
     1021 aagggaagcc cgcatcatga acaaccgcat tgcaaccaac atcagggcaa acccaatgaa
     1081 gctgaaaact atgaacatcc gggagtacgg ataatgataa aaaaagatga acaacaccat
     1141 gagcaggaaa aaggagatca ggcatttgac cgtccttttg atgaaccgca tgtaatttgg
     1201 attgccctcc tcgatataca cccctgtggc ataagccgaa ataagccaca ggatattcgc
     1261 gaccaggaac aaaagcagat atgccggacc actattccca ggtatccgcc gcagcgaatt
     1321 caacaacagc agttgaacaa ggttgagcac gataaggtct gcgcaggcga acgagaaccg
     1381 gaaaaggctg gtataacgac tgttcatagc aatgaaggtt ttggaagtaa gatgctggta
     1441 gatcgaatgt atggtctacg tgttattttc tgctgtatag ctgcttttcg taagcgtgga
     1501 taagcttcgc ccaatccact cacagatggc tatttaaaac gcatcaattg cttcatctgc
     1561 ttggcaagga aactactgga gttgtgggag gaatttcttt gggaggatgg tggacaacaa
     1621 aagaaagtga cgctaagcta atactttccc ccggaaaatg aaaggctttg gccggatttt
     1681 tttatgcctg tgaatctttt tgcgcagatg cggccgcggg aaataacagc aacgccggcc
     1741 ggttgaaata ggaagaactc gttcaggaat aatgcggtat acgtgataag cttttacaat
     1801 gacatttcgc gcctaaacag cgccatattt gaaaaaggtc caatagaaga aataacccat
     1861 gcccaatacg gccatggccc aggggtacca cgcggcgacg ggggtacgga tacggggcag
     1921 gatcagcaga aaaagtaata tgatgagggc aaggagatgc ctttcgccga tctcggtatt
     1981 gacagcgtag gccgcggcca gaaagatgca gataataaat agggtatagt cccaagagga
     2041 agacaaagac ctgcacaaaa cgataaacac gagtattccg cagcatgtga gaccgaccat
     2101 cgacaccgtc gtgatcagcg gactgatctt gccggaaagc atcattaggt gataagttat
     2161 gccggtgaac agaccggcgc cctggaaatc gctgaatacc ggcttgaaca ggattaccag
     2221 taatatagcg ccagccaacg ccaggcaaac gttacggccg gtaatacttt tatacgactg
     2281 gatcagatag gggataaaat aaaagccgat cacaaacaag atggctgtca cagtcgatgg
     2341 aatgaaggcc acgctatgaa catggaaatt gggatgcgtc aagcctttcc atcggataaa
     2401 aaccagtgca ggcgcaatca gggggaggag taaagcaatg tttccaacgg tggtttgaac
     2461 cagcgccggc aggaagggac gcctattctt atacatgatt agaatgatcc tggcaaagca
     2521 tactgccgcg ggtacaacca ggtagaattg ctggcataag acggccaggg acagacaaac
     2581 tcctgccagt aagtccctcc agtatccttt tccctctttc ccgtaaccgt tcttctttcg
     2641 ttcccgatcc agcgccagca ggcccagcaa ggcaaagaac agcccatagt tgacgggata
     2701 gaagacgaag gaattcaaaa agaagtaggg atagaaaaga agta
//
LOCUS       DATGKE010000337         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_574789, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000337 DATGKE010000000
VERSION     DATGKE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574789"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKU83_05800"
     CDS             complement(<1..412)
                     /locus_tag="VKU83_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI93098.1"
                     /translation="MKNRDKRKPISRRDFVRSTAMTTAGFYIVPRHVLGRGHVAPSDK
                     LYIAAVGTGGEAANDIKTIARTPHKNAVIAYLCDVDERPAAARRKEFPNAPFYHDWRE
                     MFDKENKHFDAVTVAIPDHNHAIVGLSAMQLKKHL"
     gene            complement(422..1126)
                     /locus_tag="VKU83_05805"
     CDS             complement(422..1126)
                     /locus_tag="VKU83_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLI93099.1"
                     /translation="MKNSILTSLVAALFALSGWHSRVATRDSALSAIDTAKWTQLFDG
                     KDLNGWKHVGPGSMSVENGLIRGHGGMGLLYWTGGKLGNCQIKVVYKMKDMNDNSGVF
                     IRIPVEPREEWMPVHYGIEVQIDNHPETSGEDEYHVTGTLYSLTKPLAKPGKPGPEWN
                     TMIITLDGPRTIVYVNGQKVTDYTEGQPVPARKFDFEPQRGRRPDSGWIGLQNHSDKD
                     IVYFKEVAERPLPGKH"
     gene            1256..1924
                     /locus_tag="VKU83_05810"
     CDS             1256..1924
                     /locus_tag="VKU83_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLI93100.1"
                     /translation="MEQHSVKILTIQDVTHNVRAYRVEKPSGYRFIPGQATEVAIDVD
                     GWREERRPFTFTGLNEDPYLEFTIKSYQDHAGVTNELYKLKAGDRLILHDVWGAIAYK
                     GPGYFIAGGAGITPFIAILRQLDREKAIGDNRLFFSNKTAADIIYHKELKGIMGDRAR
                     YVLTQESLPQFEKGPIDKSFLRNHIRDFAVNFYVCGPDKMVTDIAGALAESGAKPDTV
                     VFEK"
     gene            1995..2390
                     /locus_tag="VKU83_05815"
     CDS             1995..2390
                     /locus_tag="VKU83_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HLI93101.1"
                     /translation="METTKHDISTVADIQKLVDEFYIQVRQNPVIGPIFIGAIGDKWP
                     EHLEKMVSFWQTVIFGVPAYTGSPFPIHARMPLEERHFDTWLALWVDTVERLFAGKNA
                     YDAKQKAYNMAVMFKSKLTNPFYKASGRQ"
     gene            complement(2404..3219)
                     /locus_tag="VKU83_05820"
     CDS             complement(2404..3219)
                     /locus_tag="VKU83_05820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI93102.1"
                     /translation="MEPALQRRVQRYGWDRASRFYEAFWQAQLRPVQDLLLETASLRP
                     GEAVLDVACGTGLVSFRALEQIGNGTLLGVDISEKMIALAADLASRKGETRARFERMD
                     AEELLITDRSFDVAICALGLMYMPAPLKALEEMRRVLKPGGRVVAAVWGKREHCGWAE
                     IFEIVDRQVSSEVCPMFFHLGNQGALELNLKAAGFSGITTNRFQSTLHFRDANDAIGA
                     AFDGGPVAMASSRFTETIKARVHAEYLESIAPYKKGEGYEVPGEFVVARGVRE"
     gene            complement(3256..3774)
                     /locus_tag="VKU83_05825"
     CDS             complement(3256..3774)
                     /locus_tag="VKU83_05825"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLI93103.1"
                     /translation="MTDPEKIGSAFRRVAAALRHRPALGRGTGFSRTRITDGLTCEIH
                     EGAWTFTADMPEQAGGNAAGPTPGAYGRAALGSCLAIGYMMRAATMGIPVASLEVEVH
                     ADYDDGALFGTARGVPPGYLDVRYVVTIDSDAPEERIMELLDEADRHSPYLDVFSRAQ
                     NCQREVRIIARK"
     gene            3866..>4467
                     /locus_tag="VKU83_05830"
     CDS             3866..>4467
                     /locus_tag="VKU83_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI93104.1"
                     /translation="MSVISQFGKSNLFRAFRSKNYTLYFVGRAISQFGTWMQRTAVIW
                     LIYSITKSAFLLGVTVFAEQFPAFFFSMFGGLAADRYDRYRIVKLTQIGSMIQSVALA
                     VLVLTGHAAVWAILALSVLLGVINAFDVPARQALVHDVVAEPADLPNAISLTTATACL
                     AQLLGPAAAGLILNAYGSADCFLINAASFGGVLLSVILMTL"
BASE COUNT          946 a         1315 c         1172 g         1034 t
ORIGIN      
        1 acaggtgttt cttgagctgc atggcgctca gcccgacgat cgcatggttg tgatcgggaa
       61 tggcaaccgt taccgcgtcg aaatgtttgt tttccttgtc gaacatctcc cgccagtcat
      121 gataaaaagg cgcattggga aattccttcc gccgcgctgc cgccggcctt tcatcgacgt
      181 cgcagagata ggcgatcacc gcgttcttgt gcggcgtcct ggcgatcgtc ttgatgtcat
      241 ttgctgcttc gccgcccgta cccaccgcgg cgatatatag tttatcgctt ggggcgacat
      301 ggccccggcc aagcacatgc cggggaacga tatagaatcc tgccgtcgtc atggccgtcg
      361 agcgcacaaa gtcgcggcgg cttatcggct ttcgcttatc cctatttttc atggctgaag
      421 attaatgttt accgggcaat gggcgctccg caacctcctt gaaatagacg atatccttgt
      481 cgctgtgatt ttgcaacccg atccagccgc tgtccggcct gcgtccccgc tgcggctcga
      541 aatcgaactt gcgcgccggt accggctgtc cttccgtata atccgtcact ttctggccgt
      601 tcacgtagac gatggtgcgc ggtccgtcca acgtaatgat catcgtattc cattccggtc
      661 cgggctttcc aggtttggca agcggcttgg tcagcgagta taatgtaccg gttacatggt
      721 attcatcctc gccggaggtt tccgggtggt tgtctatctg cacctcgata ccgtagtgaa
      781 ccggcatcca ttcttcgcgg ggctcgacag gaattcggat aaacacgccc gaattgtcat
      841 tcatgtcctt catcttgtag acgaccttga tctggcaatt gccgagcttt ccgccggtcc
      901 agtacaggag tcccatgccg ccgtggccgc ggatcagtcc gttctcgact gacatcgagc
      961 ctggcccgac atgtttccag ccattcaggt ccttgccgtc aaatagttgt gtccatttcg
     1021 ccgtgtctat cgcagacagg gcggagtcgc gggttgcgac gcgggagtgc cagccggaaa
     1081 gcgcaaaaag ggccgctacc agcgaagtga gaattgaatt tttcatatcg acatgtttta
     1141 caaccgccag agctgtttct ctgttctgga cattaaactt acagaaaaat ggttgatttt
     1201 ggccaaagtt tcgtttgacg tatcaattgc gcccaagcac tcttaaattt gcatggtgga
     1261 acagcactcc gttaagatac taaccatcca ggatgtcacg cataacgtca gggcttaccg
     1321 ggtggagaag ccttcgggct atcgttttat tcccgggcag gctaccgaag tagccatcga
     1381 tgtggatggc tggcgcgaag agcgccgtcc tttcaccttc acgggtctga atgaagatcc
     1441 ctacctcgaa ttcacgatca aatcctacca ggaccacgcg ggagtcacca acgaattata
     1501 taagctgaaa gcgggcgacc ggctgatcct tcacgacgtc tggggcgcca tcgcctataa
     1561 agggcccggc tattttatag ccggcggagc cggcatcacg ccctttatcg caatcctccg
     1621 gcagttggac agggaaaaag ctatcggcga caaccggttg ttcttctcca acaaaacggc
     1681 cgcagatatc atctatcata aggagcttaa ggggatcatg ggcgaccgtg cccgctacgt
     1741 ccttacccag gaaagccttc cgcaatttga gaaggggcct attgacaaat cctttctccg
     1801 aaatcacatc agggatttcg ccgtcaactt ctacgtctgc gggccggaca aaatggtgac
     1861 ggacatcgcc ggcgccctgg cggagtcagg cgcaaagccg gatacggtgg tatttgagaa
     1921 ataactggac cgggaagacc aaataaaaga ctaaaaagtc ttttttgcaa aatgttcgta
     1981 tctttgcacc gtcaatggaa acgacaaagc atgatatttc gacggtggca gacatccaaa
     2041 aactggtcga cgaattttat atccaagtcc ggcaaaatcc tgtgatcggc cccatcttca
     2101 tcggggcgat cggtgacaag tggcctgagc acctcgagaa aatggtgagt ttttggcaga
     2161 ccgtcatttt cggcgtgcct gcgtacacgg gcagcccttt tccgattcac gcgaggatgc
     2221 cgctcgaaga aagacatttt gatacctggc tggccctttg ggtcgacacg gttgaacggc
     2281 tctttgctgg caaaaatgcg tacgatgcga aacagaaggc ctacaacatg gcggttatgt
     2341 tcaagtcgaa attgaccaat cccttttaca aggcatccgg gcggcaataa aaccttcatt
     2401 ctgctactcg cgcacccctc gcgccacgac aaattctccc ggcacctcgt acccttcccc
     2461 ttttttgtaa ggcgcaatcg actccagata ctctgcgtgg acccgagctt ttatcgtctc
     2521 agtaaaacgg ctggatgcca tggccaccgg gccaccgtcg aaagccgccc cgatcgcgtc
     2581 gtttgcatcc ctgaagtgca gcgtcgactg gaaccggttc gttgtaattc ctgaaaaacc
     2641 ggcggcctta agattcagct cgagggcccc ctgattgcca agatggaaga acatcggaca
     2701 gacctctgaa ctgacctggc gatcgacaat ctcaaagatt tccgcccaac cgcaatgctc
     2761 ccgttttccc caaaccgcag ctaccacccg gccgccgggt ttcaacaccc ggcgcatctc
     2821 ttccagggcc tttagcggcg ccggcatata cattagcccc agcgcacata tcgcgacatc
     2881 aaaggaccgg tcggtgatga gaagttcctc ggcatccatt cgctcgaacc tggcccgcgt
     2941 ttctcctttt cgggaggcca gatcggctgc cagggcaatc atcttttccg agatatccac
     3001 acccagcagg gtgccattac caatctgctc cagcgcccga aagctgacca gcccggtccc
     3061 gcacgcaacg tcaagtaccg cctctcccgg gcgcaaactg gccgtctcca gcaacaggtc
     3121 ctgcacgggc ctaagttgcg cttgccagaa cgcttcataa aatcgggagg ctctatccca
     3181 gccataccgc tggaccctcc gttgcaaagc tggttccatt caaaaaattt ttccgctgca
     3241 aaaacgacca caggatcact tgcgcgcgat gatcctgact tcccgttgac agttttgagc
     3301 gcgcgagaaa acatcgagat aagggctgtg ccggtccgcc tcatccagca attccatgat
     3361 cctttcctcc ggcgcgtcgc tgtcgatcgt gacgacatac cgcacgtcca ggtacccggg
     3421 tggtacgccg cgggcagtac cgaatagcgc cccatcgtcg tagtccgcat gcacttcgac
     3481 ttccaggcta gccacgggaa tgcccatcgt cgcagccctc atcatatacc cgatggcaag
     3541 gcaacttccg agagccgcac ggccataagc tcccggggta ggcccggcag cattaccacc
     3601 cgcttgttcg ggcatgtcgg cggtaaaggt ccaggctcct tcatggatct cgcaggtcag
     3661 gccgtcggtg atccttgtcc tcgagaaacc ggttccgcgg ccaagggcgg gacgatgcct
     3721 aagcgctgcc gccacccgac ggaaggcgga gccaatcttt tccgggtcgg tcatgtgcaa
     3781 tatttggaga aagttacaaa ctatttggag ggttcctttg gcctgcctgt cccatatcca
     3841 tgagatcgaa gtatttttgc atcgaatgtc agtaatcagc cagttcggga agtcgaatct
     3901 tttcagagca ttccggagca agaattatac actgtatttt gttggaaggg ctatctcgca
     3961 gtttggtacc tggatgcagc gcacggcggt catctggctc atatattcca tcacgaagtc
     4021 ggcctttctt ctgggcgtca ccgtcttcgc ggaacaattc cctgctttct ttttttcgat
     4081 gtttggcggc ctcgcagccg accggtatga ccgctaccgc atagtcaagc tgacgcagat
     4141 cgggtcgatg atccaatcgg tggccctggc agtgctggtg ctcacaggtc acgccgctgt
     4201 ctgggcgata ctggccctga gcgttttgct gggcgtcatc aatgcctttg atgtgcccgc
     4261 ccgtcaggcc ctcgtgcatg acgtcgtggc ggagccggcc gacctgccga atgcgatctc
     4321 cctgacaacg gcgaccgcct gcctggcgca gctattggga cccgcggcgg ccggtctcat
     4381 ccttaacgct tacggctcag cagactgttt cctcatcaat gccgcaagct tcgggggggt
     4441 cctgctttcg gtgatcctca tgacgct
//
LOCUS       DATGKE010000338         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_939210, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000338 DATGKE010000000
VERSION     DATGKE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939210"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VKU83_05835"
     CDS             <1..505
                     /locus_tag="VKU83_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HLI93105.1"
                     /translation="EARAAGEKLRAIRFDHGFTSVLQRAIDTMDILLEAAKQPQLPVT
                     RDRALNERNYGRLQGLNKMAVAGQYGEAQVALWRRSFSERPPGGESLADTAARVIPYY
                     AKAIEPLLRQGRDILVVAHGNSLRALVMHLEHISEEAIAGFDLPTGAPRCYRLNNSLQ
                     IDGVAYL"
     gene            565..1188
                     /locus_tag="VKU83_05840"
     CDS             565..1188
                     /locus_tag="VKU83_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93106.1"
                     /translation="MRLTFSWMCRLVLAVMIALIIWAVPGCSKNTPNDNKKDSVDTPR
                     PKVYDSAAYYVSFLINIYNTSGVFTDTFSDDASMKIYIVNGVVKIPHDSILNFPPIVF
                     PSSGKDGFYKATWIPDNIGEINITAATGIMDPGDSLCTIILTHSGTVTPKWQLTALVG
                     GTTTYAGGESTPGWPPGFTFKTKLQSQDAFKLDQPGSYWDVWVYKDY"
     gene            complement(1239..>2508)
                     /gene="bshC"
                     /locus_tag="VKU83_05845"
     CDS             complement(1239..>2508)
                     /gene="bshC"
                     /locus_tag="VKU83_05845"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HLI93107.1"
                     /translation="KILHTIRIAEWLKEQHPQLHFVPVFYMGSEDADLEELGHIYLGG
                     EKLVWETKQTGAVGRMTTKGLDKLFYRLEGELSVQPYGPELMTLLKAAYLESPDIQTA
                     TFRLLHSLFADRGLVVLIADKAAFKNQMLGVFEDDLFREEPSRIVSETIARLGEHYKV
                     QANPRPINLFYLKDDLRNRIERTAEGFVVVDTKLRFTEQELREELHRHPERFSPNVIL
                     RGLFQETILPNLAFIGGGGETAYWLELKALFDHYRTPFPVLVLRNSFLLVQKHWMEKL
                     RSAGFDIPDLFRGGEELVNELVQRDSDHRLSLEEEIAAANNYYEALKALARPVDPTLE
                     QHVTALQVRALEPIRSLEKKLLKAEKRRFSEQQRQIQTLKAALFPHDNLQERVENILP
                     WYAAYGPQILDELYRQSPAIEQQFVVLTEQ"
BASE COUNT          529 a          726 c          673 g          580 t
ORIGIN      
        1 ggaagcccgg gccgcaggcg agaagctccg tgccatccgc ttcgaccatg gctttaccag
       61 cgttctgcaa cgcgctatcg atacgatgga catcctgctt gaagcggcca agcagccgca
      121 gctaccggtg acgcgcgacc gcgcattgaa cgagcgcaat tatggccggt tgcaggggtt
      181 gaacaaaatg gcggtggccg ggcaatacgg cgaggcgcag gtggcgctct ggcgacgcag
      241 cttttccgaa cggccgcccg gtggcgaaag tcttgccgac actgccgccc gggtcatccc
      301 ctattatgcg aaggccatcg agcccctgtt gcggcagggc cgcgatatcc tggtggtcgc
      361 tcacggcaac agccttcgcg ccctcgtcat gcacctggaa cacattagcg aagaagcgat
      421 cgccggtttc gatcttccca cgggagcccc gcgttgttac cggctcaaca acagcctgca
      481 gatcgacgga gtagcctacc tctagtactt tcgttctatt gccggggagg tgatacctga
      541 gtagttttgg caaaactctc ccctatgcgg ctgacttttt cgtggatgtg ccgtctcgtc
      601 ctggcggtga tgatcgcgtt gatcatttgg gcggtgccgg gctgttccaa aaataccccg
      661 aacgacaata agaaggactc cgtggatacg cccagaccca aggtctacga cagcgctgcc
      721 tactatgtgt cattcctgat caatatctac aataccagcg gtgtttttac agataccttt
      781 agcgacgatg cgagcatgaa gatctatatt gtcaatggag tggtgaagat cccgcacgac
      841 agcatcctca attttccacc gatcgttttc ccttcttccg gcaaagacgg cttttataag
      901 gcgacctgga tccccgataa catcggcgag atcaatatca ccgccgctac cgggatcatg
      961 gaccccggtg acagcctttg caccattatt ttgacccata gcggcaccgt cacaccaaaa
     1021 tggcagctca ccgcccttgt tggcggtacc actacctacg ccggcggcga atccactccc
     1081 ggctggccgc ctggctttac ctttaagaca aaacttcaaa gccaggatgc gttcaagctg
     1141 gatcagcccg gcagttattg ggacgtctgg gtgtacaaag attattgaaa gtgcaccgac
     1201 caccggcagc gctgtgaaaa tcgttcctga tgtcttgctc attgttccgt gagcactacg
     1261 aattgctgtt cgatcgccgg ggattgacga tagagctcgt ccaggatctg cgggccatac
     1321 gccgcatacc acggcaggat attctcaact ctttcctgaa ggttatcgtg cgggaacaag
     1381 gcggctttga gcgtctggat ctggcgctgc tgttcgctga accgtcgttt ctctgccttg
     1441 agaagctttt tttccagcga acggatcggt tccagcgccc tgacctgtaa cgccgtcaca
     1501 tgttgttcca atgtggggtc cacgggtctt gccagggctt ttagcgcctc gtagtaattg
     1561 ttggcggcag cgatctcctc ttcgagactt aggcggtgat cggaatcgcg ttggaccagc
     1621 tcattgacca attcctcccc gccgcggaac agatcaggga tgtcgaagcc ggcactccgg
     1681 agcttctcca tccaatgttt ttgcacgagt aaaaaagaat tgcgaagcac taaaacaggg
     1741 aaaggcgttc ggtaatgatc gaacagcgct ttcagctcca gccaataagc cgtttcgccg
     1801 cccccgccga tgaaggccag attaggcagg atcgtctcct gaaaaagtcc ccgcaggatg
     1861 acattcgggc tgaatcgttc cgggtgacgg tgtagctctt cgcgcagttc ctgttcggta
     1921 aaacgtagtt tagtatccac gacaacgaaa ccctccgccg tgcgctcgat ccggttgcgc
     1981 agatcatcct tcaaatagaa caaattgatg ggccggggat tggcctgtac tttatagtgt
     2041 tcgccaagcc gcgcgatcgt ttcgcttacg atcctgctcg gttcctcgcg gaaaagatcg
     2101 tcttcgaaca cgccgagcat ttggttttta aaggcggctt tatccgctat caaaactacc
     2161 agtccgcggt ccgcaaacag gctatgcagc agccggaacg tggcggtctg aatatccggg
     2221 ctttccagat aggcggcctt caacagcgtc atcagctccg gaccatacgg ctgaacggac
     2281 agctcgcctt ccaggcgata gaacagcttg tccagccctt tggtcgtcat gcggcctacc
     2341 gctccggttt gtttcgtttc ccataccagc ttctctccgc ccagatagat atgccccagc
     2401 tcttcgagat ccgcgtcctc gctgcccata tagaatacgg gtacaaaatg cagctgcgga
     2461 tgttgttctt tcagccattc ggcgatgcgg atggtgtgca ggatctta
//
LOCUS       DATGKE010000339         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_750189, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000339 DATGKE010000000
VERSION     DATGKE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750189"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1652
                     /gene="nirB"
                     /locus_tag="VKU83_05850"
     CDS             <1..1652
                     /gene="nirB"
                     /locus_tag="VKU83_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HLI93108.1"
                     /translation="AQGMIYGLVAPGYEMADVAVSNLTGGDRRFTGYDLSTKLKLIGV
                     DVASFGDPFVAEPDCRTIVYEDRHKGVYKRINISPDGKFLKGGVLIGDASAYHLLLQT
                     VNNKMLLPADPEDMILGARGAAAEGGGVMSLPDETLVCSCESVNKVTICAAVTERSAT
                     TVEAMKKCTRAGTGCGGCLPLVKDLIAGTMKMGGMYVRNVVCEHFNYSRQELFDLVKL
                     HDVRNYDDVLHRWGKGDGCEICKPAVASILASCWNEPIVKQAPIQDSNDRYLANIQKG
                     GSYSVVPRVPGGEITPEKLIVLGKVARKYNLYTKITGGQRIDLFGAHVSDLPSIWEEL
                     IAAGFESGHAYGKALRTVKSCVGSTWCRYGLHDSVSFAIEIEERYRGIRAPHKIKGGV
                     SGCVRECAEAQSKDFGIIATEKGWNLYVCGNGGSRPQHAQLLAVDIDKETCVRYLDRF
                     LMFYIRTADPLTRTATWLNKMEGGMSYLKNVVINDSLGMGKKWEEEMQVLVDSYHCEW
                     AAVVNNPELRKQFVHFVNAPKKKDPELRFEEMRGQVKARNW"
     gene            1663..2019
                     /gene="nirD"
                     /locus_tag="VKU83_05855"
     CDS             1663..2019
                     /gene="nirD"
                     /locus_tag="VKU83_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HLI93109.1"
                     /translation="MIDVPIKWMAACREEDVPAEGGACVLLGGEQIAIFRFSRRSEWY
                     ATQNLCPHKQQMILSRGMTGSTGEGCEPKVACPFHKKTFSLRTGSCLSGEDYQIRTYP
                     VRVKAGKVYVGFTQPD"
     gene            2052..3002
                     /gene="moaCB"
                     /locus_tag="VKU83_05860"
     CDS             2052..3002
                     /gene="moaCB"
                     /locus_tag="VKU83_05860"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HLI93110.1"
                     /translation="MRDITPKQVSLRTARAAGIIFCAAETISLIREDKLPKGNLFDVA
                     RAAGFLGAKLTPQLLPHCHPVGIDGMEMQFEFLDSLQHGELFGAEAAARPGIVIRGEA
                     KSIGRTGIEMEVLTGVSVAALEIYDMLKPVDKHLEIGCIKLVEKSGGKSDREKYFAMS
                     PTCAVLVCSDSTAAGKREDRSGRVVVGMLEAARAVVRHYAVVPDDKLRIQTQIRNWVA
                     EDIQFVFTTGGTGLGPRDNTVAAVAEILERDANGIAEAMRVHGQMRTPLAMMSRGVAG
                     SIGETLIVTLPGSSDGARESLEAILPAVFHARKMMKGGGH"
     gene            3004..>3358
                     /locus_tag="VKU83_05865"
     CDS             3004..>3358
                     /locus_tag="VKU83_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93111.1"
                     /translation="MLSFQEARELILAQARSWGTEAVPLGEAFGRVLAETVRADRDYP
                     PFPRATMDGYAMRFDDLDRGIRRFRVVETLFAGGRPVAPLESGDCYKIMTGAAVPNAA
                     NIVIRREDVTEKGTEM"
BASE COUNT          749 a          814 c         1127 g          668 t
ORIGIN      
        1 tggcccaggg gatgatctac gggctggtcg cgccgggata cgaaatggcc gacgtcgcgg
       61 tatcgaatct aacgggcggg gacagacggt ttacgggcta tgacctgagt acgaaactca
      121 aactgatcgg ggtggacgtg gccagtttcg gcgatccttt cgtggccgag ccggactgcc
      181 ggaccattgt ttatgaggat cgccataagg gagtatacaa gcgaatcaat atcagtccgg
      241 atggaaaatt cctgaaaggc ggggtattaa tcggggatgc atccgcgtat cacctgcttc
      301 tgcagacggt caacaataag atgctgttgc cggcggatcc ggaagatatg atcctgggtg
      361 cgcggggggc cgccgccgaa ggcggcgggg tgatgagcct tcccgacgag accctggtct
      421 gttcctgtga gtccgttaat aaggtgacca tttgcgcagc cgtgaccgaa agatcggcca
      481 caaccgtgga agcgatgaag aaatgcaccc gcgccggcac gggttgcggc ggctgtttgc
      541 cgctcgtcaa agatctgatc gccggaacga tgaagatggg agggatgtat gtccggaatg
      601 tggtttgcga gcatttcaat tattcgcggc aggagttgtt cgacctcgtc aagctccatg
      661 acgttcgcaa ctatgacgat gtgttgcacc gctggggaaa aggtgacggt tgtgagatct
      721 gcaagccggc ggtcgcgagc attctggcga gttgctggaa cgaaccgatc gtgaagcagg
      781 cgccgatcca ggatagcaat gaccgttacc tcgccaatat ccaaaagggc ggcagctatt
      841 cggtcgttcc gcgtgttccg ggcggcgaga tcacgcccga aaagctgatc gtgctcggaa
      901 aggtggccag gaaatacaat ctgtacacga agattacggg tggccagcgg atagatcttt
      961 tcggcgcaca tgtgtccgac ctgccgtcca tctgggagga gctgatcgcc gcgggttttg
     1021 aaagcgggca tgcttatggt aaggcgttgc ggaccgtcaa gagttgtgtc ggtagtacct
     1081 ggtgccgtta tgggctgcat gacagcgtgt cgttcgccat cgaaatcgag gagcgctacc
     1141 gcggaatccg ggcgccgcac aagatcaaag gaggggtcag tggctgcgtc cgcgaatgcg
     1201 ctgaggcgca aagcaaggat ttcgggatca tcgccacgga aaaaggctgg aatctttacg
     1261 tctgcggaaa cggcggatcc aggcctcagc atgcacagct gctcgcagtc gacatcgaca
     1321 aggagacctg cgtgcggtac ctcgaccggt ttttaatgtt ttacatccgg acggcggatc
     1381 cgctgacgcg aacggccacg tggttaaaca agatggaggg gggaatgagc tatctcaaaa
     1441 atgtggtcat caacgacagc ctgggcatgg gaaagaagtg ggaggaggaa atgcaggtac
     1501 tggttgacag ttaccactgc gaatgggcgg cggtagtaaa caatcccgag ctgcggaaac
     1561 aattcgtgca ttttgtgaat gcgccgaaga agaaggaccc tgaattgcgc ttcgaggaga
     1621 tgcggggcca ggtgaaagcc aggaactggt aaaaaaatct ctatgataga tgtaccgatc
     1681 aaatggatgg ccgcctgccg ggaggaagat gtacccgcag aaggcggcgc gtgtgtcctg
     1741 ctcgggggcg agcagatcgc tattttccgt ttttcgcgcc ggtcggagtg gtacgctacg
     1801 cagaaccttt gcccgcataa gcagcagatg atcctgtccc ggggaatgac cggaagcacg
     1861 ggagaaggat gcgagccgaa agtggcctgt ccatttcata aaaagacgtt ttccctccgg
     1921 accggatcct gtttaagcgg agaggattac cagatcagga cctaccccgt ccgcgtgaag
     1981 gccggaaagg tatatgtcgg atttacgcag ccagattgaa agaattgcgc atttttgcct
     2041 aaaattcttt catgcgggat attacaccta agcaggtctc gctgcgaacg gcccgtgcgg
     2101 ccgggatcat cttctgcgca gcggagacta tttcacttat ccgcgaggat aaattgccga
     2161 agggtaacct ttttgatgta gcgcgcgcgg cggggttcct gggggcgaag ttgacgccgc
     2221 agttgttgcc ccattgtcac ccggttggga tcgacggcat ggagatgcaa ttcgagttcc
     2281 tcgacagtct gcaacatggc gaactgtttg gcgcagaggc ggcggctcgc ccgggtattg
     2341 tcatccgtgg ggaagcgaag tcgatcggac gtaccggtat tgaaatggaa gtgctgacgg
     2401 gagtctccgt tgcggcgctg gagatctacg atatgctcaa gccggtcgac aagcatctcg
     2461 agatcgggtg tatcaaattg gtcgaaaaga gcggcgggaa gagtgaccgg gaaaaatatt
     2521 ttgcgatgag cccgacctgc gccgtgctgg tgtgttcgga ttcaacggca gcgggaaaaa
     2581 gagaggaccg cagcggccgg gtcgtagtcg ggatgctgga ggcggcccgc gccgtcgtcc
     2641 gccattatgc ggtcgttccc gatgacaagc ttcgcatcca gacacagatc cggaattggg
     2701 ttgccgagga cattcaattc gtttttacga cgggcgggac gggcctcggg ccaagggaca
     2761 atacggtcgc ggcggttgcc gagatcctcg agcgcgacgc gaacgggatt gcggaagcca
     2821 tgcgggttca cgggcagatg cgaacgccgc tggcgatgat gagcagggga gtcgccggct
     2881 ctattgggga aaccctgatc gtcaccttgc ctggcagctc ggacggagcg cgggagtcgc
     2941 tcgaggccat cctgccggcg gtatttcacg cgcgaaaaat gatgaaaggg ggaggacact
     3001 gacatgctga gttttcagga agcgcgggag ttgatactgg ctcaagccag gtcgtggggt
     3061 acggaagccg tgccgcttgg agaggctttc gggcgcgtat tggcggagac ggtgcgagct
     3121 gatcgcgact atccgccgtt cccacgggct actatggatg gatatgcaat gcgttttgac
     3181 gacctggatc ggggaatccg ccgtttcagg gtggtggaaa ccctcttcgc aggcgggcga
     3241 ccggtagccc cgcttgaaag cggggactgc tataaaataa tgacgggggc tgcagtgccg
     3301 aatgcggcga acatcgttat ccggcgcgag gacgtgacgg aaaaaggaac ggagatgg
//
LOCUS       DATGKE010000340         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_8674, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000340 DATGKE010000000
VERSION     DATGKE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8674"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VKU83_05870"
     CDS             <1..1406
                     /locus_tag="VKU83_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI93112.1"
                     /translation="RKALGYVVSIRAEYDWLRLRGLNYQYSQSYGRNPALTNPVVYPQ
                     QPSGQWDSIFYNYRSTVHELSLQGVFTFNNINFHKAKTGFNWYLFGGIGAMTYSTLYK
                     ADASYLNGFRNVIDAYPIENHSEKKKIRDMLKGVLTGKWNQIAERPGNSSMLGGVPVD
                     IVGVVGIGAQFKLNDRLNIQLEDKVTLTTTKLLDGEQWQNNYTFGATNSVAQVGSADR
                     YNYASVGINYNIFAKHAVEPLWWLNPLDYAYNELNAPRHMKLPKPVLDDADGDGVTDQ
                     FDLEPNTPKGCPVDTHGVSLDTDGDGVPDCRDKEKITPTMCQPVDADGVGKCPDPQCC
                     NDLKHLIDSLGVGHGKCNIGNLPSVTFKGHAVTLSKDNQALLASAASQMRNNPSCKIA
                     VIGYGESSKAAQQLSWDRVNAVINYLVEKEGISSDRFIFRYGQSGGDESTVDLRDGTG
                     EEGPNAVPAPHPNLRRR"
     gene            1640..2455
                     /gene="bamD"
                     /locus_tag="VKU83_05875"
     CDS             1640..2455
                     /gene="bamD"
                     /locus_tag="VKU83_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HLI93113.1"
                     /translation="MRLFAIALVGIFLFTGCSRFSKVMKSNDYDYKLRVADKYYADKD
                     YNHAQQLFEELFRVFKGSEHFEDIYYKFAYCAYYLKDYPNAENLFKGFVEVFPNSNKA
                     EEMDYMRAYTFYMESPKVELDQTNTTKTIGFMQAFINTHPGSDKIKEATDIIEKCRAK
                     LELKDFLSAQLYYDLGEFKAAAVALNSLMTSYPDSEKSDQYKLLIIKSDFRYAELSIY
                     SKQEERYDAVVTQCNDFLDRFPDSKLKPDVQGYLTSAQNNLKTFKNEQTKKAS"
     gene            2430..2771
                     /locus_tag="VKU83_05880"
     CDS             2430..2771
                     /locus_tag="VKU83_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLI93114.1"
                     /translation="MSRLRRQVNANTANVVETKNLTDIKARTGNLYESIAIIAKRANQ
                     INISLKEELHNKLEEFATHTDSLEEIHENKEQIEISKAYERMPNPALLATQEFMDDKI
                     YYRRNDDDLFK"
BASE COUNT          743 a          699 c          780 g          650 t
ORIGIN      
        1 tgagaaaagc tctgggctac gtggtctcta tccgggccga gtatgactgg cttcggctca
       61 gaggtcttaa ttaccaatat tcgcaatctt acgggcgcaa tcctgctttg accaatccgg
      121 tagtgtatcc ccagcagccg agcggtcagt gggattccat cttctacaat tatcgttcaa
      181 cggtccatga attgtccctc caaggtgtct tcacgttcaa taatataaat ttccacaagg
      241 ccaagaccgg atttaactgg tatttatttg gtggcatcgg cgccatgacc tacagtaccc
      301 tgtacaaggc cgatgcgagc tatctcaacg gtttcaggaa tgtcatcgat gcttacccca
      361 ttgaaaatca cagcgagaaa aagaagatcc gcgacatgct caagggggtg ttgaccggca
      421 aatggaatca gattgccgag cggccgggga acagttcgat gctgggcggc gtccccgttg
      481 atatcgtcgg agtggtcggt attggcgccc agttcaaact gaatgaccgg ctgaacattc
      541 agctcgagga taaagtgacc ctgacaacga cgaagctgct ggatggcgag cagtggcaga
      601 ataactacac gttcggtgcg acgaacagcg tggcgcaggt aggatcggct gaccggtaca
      661 attatgcttc cgtcggcatc aactataata ttttcgccaa gcatgcggtc gagccgctgt
      721 ggtggctgaa cccgctcgac tatgcctaca acgagctcaa cgctccccgt cacatgaaac
      781 tgccgaagcc ggtgctggat gacgcggatg gcgacggcgt taccgaccag ttcgacctcg
      841 agcccaatac tccgaagggt tgccctgtcg atacgcatgg ggtgagcctc gataccgacg
      901 gtgacggagt tccggattgc cgcgataagg agaagatcac gccgaccatg tgtcagcccg
      961 tcgatgccga tggtgtaggg aaatgcccgg atccgcaatg ctgcaacgac ctgaagcacc
     1021 tgatcgattc gctgggcgta ggtcatggaa agtgcaatat cggcaacctt ccgagtgtca
     1081 ccttcaaggg ccacgcggtt acgctgagca aggacaacca ggcattgctg gccagtgccg
     1141 cgtcccagat gcggaacaat ccgagctgca agatcgccgt aatcggttat ggcgagtcga
     1201 gcaaggccgc tcagcagctg agctgggacc gggtaaatgc cgtcatcaat tacctggttg
     1261 aaaaagaagg tattagctcc gaccggttca tcttccgcta tggtcaaagc ggcggcgatg
     1321 aaagcacggt cgatctgcgc gacggtaccg gggaagaagg accgaatgcg gttcccgcac
     1381 ctcacccgaa cctgcggcgg cggtagagcc attcgctttg aatgaatagt atagcgcggg
     1441 ccggacggcc cgcgctattt tttttgccgg tgacttcgca ttcttttctt gccggacttc
     1501 gcattctttt cctgccgtgg cttcgcattc ttttttgggg cgactttaac gagctttttt
     1561 tgggtccggg cgcaaaaccg tgcatttttg cccttacaga ctgctatttg cttaaaaaac
     1621 attaactttg cctgctttta tgcgtctatt tgccattgct ttagttggaa tattcctgtt
     1681 taccggctgt tcgcgctttt cgaaggtgat gaagagcaac gattatgact acaaacttag
     1741 ggtagcggac aagtactatg cggacaagga ttacaatcac gcgcagcagc tatttgaaga
     1801 gctattcagg gtcttcaagg gcagtgagca ctttgaggac atctattata agtttgccta
     1861 ttgcgcgtat tacctgaagg attacccgaa tgcggagaac ctcttcaagg gcttcgtgga
     1921 ggttttcccg aatagcaaca aggccgagga aatggactat atgcgggcct atacgtttta
     1981 tatggagtcc ccaaaagtgg agttggatca gacgaatacg acgaagacga tcggctttat
     2041 gcaggccttc atcaatacgc accccggctc ggacaagata aaggaagcta cggatatcat
     2101 cgaaaaatgc cgggcgaaac tggagctgaa ggattttctg agcgcccagc tctattacga
     2161 cctgggtgag ttcaaggctg cggcggtcgc gctcaatagc ctgatgacga gctatccgga
     2221 ctcggagaaa agcgatcaat acaaattgtt gataatcaag tctgatttcc gatatgccga
     2281 attgagcatc tattccaagc aggaggaaag atatgacgcg gtagtaacac aatgtaatga
     2341 tttcctggac cgttttccgg acagcaaatt gaagccggac gtgcagggat atctgacctc
     2401 ggcgcagaac aatttaaaaa cctttaaaaa tgagcagact aagaaggcaa gttaatgcga
     2461 acacggccaa cgtcgtggaa acgaagaacc tgaccgatat caaggccagg acggggaatt
     2521 tgtatgaatc gatcgcgatc attgccaaaa gggcaaatca gatcaatatt tccctgaagg
     2581 aggagttgca caataagctg gaggagtttg ccacgcacac ggatagcctt gaggagattc
     2641 atgagaacaa ggagcagatc gagatctcga aggcgtatga acggatgccg aacccggcgc
     2701 tcctggctac ccaggaattt atggacgaca agatctatta ccgcagaaat gacgacgacc
     2761 tcttcaaata aggatcggta agacgacatg atttacggca gggcgaccca tgggcgccct
     2821 gccgttttgc tctaaaaacc ttaatcaacg gaccatgttt cagggaaaaa aa
//
LOCUS       DATGKE010000341         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_282697, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000341 DATGKE010000000
VERSION     DATGKE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282697"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1582
                     /locus_tag="VKU83_05885"
     CDS             386..1582
                     /locus_tag="VKU83_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLI93115.1"
                     /translation="MQRPPLPLNEVERIADLYEYDILDTTSEEDFENIARLAASICNT
                     SMAAISFVDAERQWFKARHGVFFRQTSRKHSFCAHTILNEGFFIVRDTLDHEVFRQFP
                     YVTGGPRIRFYGGIALHSHRGHRIGSLCVMDDSPRFLDGYQQRALSELSRQVSQLLEN
                     RRLRKRLKRITQVQNRFQELLVKEVKSSLGATGTFLNALENYGPELIPGPGFCSQIAG
                     QFQRTLAIFDCLVQWRNVQELDRYGIWPTVDAHRMATDVLRRTKREPCAKGFSFVNTS
                     QSIEHRPLPDDMIVFILKLLLLWICDVAEDGEIDFHLLTVEDARLKMGISLRSRSFLP
                     HLRRKLEKLEKQLNGPLWSEALQSPVDNLCLLLAKDVLNGWNGELSLNLGAGLLDATI
                     DIPLNR"
     gene            1620..>2653
                     /locus_tag="VKU83_05890"
     CDS             1620..>2653
                     /locus_tag="VKU83_05890"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLI93116.1"
                     /translation="MTKLFTCLPLLAATFIQSSHAQTRSAQPANSPLPAIIEKHGRHT
                     LMVDGKPFFLLGGQAHNSSAWPPLLPGVWQAIAEMNANTLEIPIYWEQIEPDKDRFDF
                     SLVDTLIGQALRHHVRLVLLWFATWKNGSNHYMPGWMKHQPDTYPNCIASNGQWVDSP
                     SPIANATLEADKKAFAAVMRHLRSEDPQHTVIMVQVENEPGAWNTVRDYSPAAQKLFA
                     GPVPSALLKPDILQQLGHPGAAGGSWQQVFGSDADEYFHAWYVALFIGQVAAAGKAED
                     PLPMYVNAALRDPLTHPAASTYESGGATDNVIPIWKAAAPAIDLVAPDIYLSGNDKVS
                     RVVQLYTRRD"
BASE COUNT          601 a          870 c          639 g          543 t
ORIGIN      
        1 ccgctcacgg atactcaaga agctgcgcct cagcagtaac gccgagctca cccggtacgc
       61 cctccaccat cagttgatcg actaactccc cctacttgca gcccttattt taacccggcc
      121 tttgtaggag atcgacgaca aacaccccgc aggaaaaggg acaggaatcc tgcgcctaga
      181 gctattgccc gccaacctat cctgtcgttc ctttgcatcc cgataaaatc ggttacatta
      241 tttcgaaaaa ccaagtatag cagcagcccg agatcccgcc ctctcgaaaa agatccgtcc
      301 cgtccgtgcc ctgttgaaaa ctattgacga gaacgcttgc caggaacgct gtcgataaaa
      361 accgcctctt cgtcaaaatg attttatgca aagaccgcct ttaccgctga atgaagtaga
      421 acggatcgcc gacctctacg aatacgatat cctggataca acgtccgagg aggatttcga
      481 gaacatcgcg cgtttggccg cctcgatatg caatacctcg atggcagcca tcagcttcgt
      541 agatgcggaa agacaatggt tcaaggcccg tcacggcgtt tttttccgcc aaaccagcag
      601 aaaacactcc ttctgcgcgc acaccattct caatgaaggc tttttcatcg tacgcgacac
      661 gctggaccac gaggtcttcc ggcaattccc gtatgttacc ggcggtccgc ggatcaggtt
      721 ctatggcggc atcgccttgc attctcatcg cggtcaccgc attggttcgc tttgcgtgat
      781 ggacgatagc ccgcggttcc tcgacggcta tcagcaacgc gccctgtcgg agctaagccg
      841 gcaggtcagc cagctgctcg agaacagaag attgcgtaag cggttgaaac ggatcacgca
      901 agtgcagaac cggttccagg agctgctcgt caaagaggtc aaaagttcgc tcggcgctac
      961 cggcaccttc ctcaatgcac tggaaaacta tggccccgag cttatccccg gtccgggttt
     1021 ctgctcgcag atcgccggac aattccagcg gaccctcgct attttcgact gcctcgtgca
     1081 gtggcgaaat gttcaggaat tggaccggta cggtatctgg cctaccgtcg atgcccaccg
     1141 gatggccacc gatgttttac gccggacaaa acgcgaacca tgcgctaaag gcttttcttt
     1201 cgtgaacacc agccaatcca tcgaacaccg gccccttccc gacgacatga tcgtctttat
     1261 ccttaagctc ctccttttgt ggatttgcga tgtggcagag gatggcgaga tcgattttca
     1321 tctcctgacc gtcgaagacg cccggctaaa aatgggcatc tccctgcgct ccaggtcatt
     1381 cctcccccat ctccgccgga agctcgaaaa gctggagaaa cagctcaacg gccccctctg
     1441 gtccgaagcc ctccaaagcc ccgtcgataa cctctgcctg ctgctggcaa aagatgtcct
     1501 caacggatgg aacggcgagc tgtccctcaa cctgggagcc ggcctcctcg acgccaccat
     1561 cgacataccg ctgaatagat agaaagctat atcttggaga cccaaaacaa aggcctccca
     1621 tgacaaaatt gttcacctgc ctccccctgc tggctgccac cttcatccaa agctcccacg
     1681 cccaaacccg tagcgctcaa cccgcgaact cccccctccc ggccatcatc gaaaagcacg
     1741 gccgccacac cctgatggtc gacggcaaac ccttcttcct cctcggtggt caggcccata
     1801 actccagcgc ctggcccccc ctccttcccg gcgtctggca ggcaatcgca gagatgaacg
     1861 ccaataccct cgagatcccc atctattggg aacagatcga gcccgataag gaccggttcg
     1921 atttctccct cgtagataca ctgatcggac aggccctccg gcaccacgtt cgcctcgtgt
     1981 tgctttggtt cgccacatgg aagaatggca gcaaccacta tatgcccggc tggatgaaac
     2041 accagccaga cacctatccg aactgcatcg cctctaacgg tcaatgggtc gattccccct
     2101 cccccatcgc caacgccacg ctcgaggccg acaaaaaagc gttcgctgca gtaatgcggc
     2161 atcttcgaag cgaagacccg caacatacgg tgattatggt ccaggtcgag aacgaacccg
     2221 gcgcctggaa taccgtccgg gactattcgc ccgcagcgca aaaacttttc gcaggtcccg
     2281 ttccttcggc actgttgaaa ccggatatcc tgcaacagct cggtcatccc ggcgctgccg
     2341 gcggctcctg gcaacaggtg tttggcagcg atgccgacga atatttccat gcctggtatg
     2401 tcgccctttt catcggtcag gtagctgcgg caggtaaggc ggaagatcct ctccccatgt
     2461 atgtaaacgc cgccctccgg gatccgctta cccatcccgc ggcctccacg tacgaaagcg
     2521 gcggcgccac cgataacgtg atccccatct ggaaggccgc ggcaccggcc atcgatctgg
     2581 tggcccccga tatctatctt tcaggtaacg acaaggttag tagggtcgtc cagctgtaca
     2641 ctcgccgcga taa
//
LOCUS       DATGKE010000342         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1266714, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000342 DATGKE010000000
VERSION     DATGKE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266714"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VKU83_05895"
     CDS             <1..1557
                     /locus_tag="VKU83_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HLI93117.1"
                     /translation="WVLAALVRVLSWLPVDDPHRQEYLQDYRDMVKALAPLQRRDGYW
                     NVSLLDSTHFGGKELTGTALFTYGISWGIREGWLDGKTYLPVVARAWNAMVSGSLHHD
                     GMLGYVQGTAERPADGQPLDYDKRPNVEDFGLGCFLLAGSEVYQIAEPLVANETEAKV
                     PPYREPDLHLSTAKEWEERQRKEVHKAFAADVYGRMPDVTIPVGRRYGVIDSTALDGL
                     AIKKTITLDFLPGDTAGDTTARLHMLLYLPKTVRRAPVFLGYSFTPNDVTEHAPQWPL
                     KEIVRRGYAVAVAWYWDIEPDRPDGWQTGIRTRLAGLLHIEPYEWGAIGAWAWGMERM
                     ADELEAETGIDPKRIIAIGHSRLGKTALWAGANDARFAMVISNESGEGGAAMARRDYG
                     ETIAMINDRFPWWFSPAYKQFGADPSAMPVDQYELLALIAPRPLYIASAMGDQWSDPR
                     GEFLSAKLAGQVYRLYGERCVGVDSMPPVDHPVGSFIGYHIRTGKHDLTLYDWERYLD
                     FAARRLKAGK"
     gene            1608..2870
                     /gene="fucP"
                     /locus_tag="VKU83_05900"
     CDS             1608..2870
                     /gene="fucP"
                     /locus_tag="VKU83_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HLI93118.1"
                     /translation="MPKRNLIFPFILVCTLFFLWAFLHNINGILIPKLKKVCDLSDTQ
                     SSFIDVAVYLGYFVFAIPAGLVSHRFGYKKGILTGLFLFALGALLFLPAADIRSYGLF
                     LVALFVAASGATFLETVANPYAANMGSPENPEQQLNFAQSFNGLGAIVAPLIGERYIL
                     SGIEHSKGELAAMQAHGQLTAYLQQEANAIKPPYLIMAAVLVLVAIAFLFTKLPEIRQ
                     TDGAHGTEFSIRVLRFPHVRWAVIAEFFYVGAQVGVTSFLVRFSKYAANIGEKEAGKM
                     LTLAMAGFMAGRFLGTFFMRYIRPASLLSLYALINIGLLMVVLAGHGKVAVYALMTIP
                     FFLSIMFPTIFALGIRDLGEETKLASSFLVMSIIGGAFTPFMMGLISDHTGGDIQVAY
                     IMPVVCMGFILWFGLRGHRVRNFQPALA"
BASE COUNT          576 a          778 c          928 g          687 t
ORIGIN      
        1 tgggtcctgg ccgcacttgt ccgggtactg tcctggctgc ccgtagatga tccgcaccgg
       61 caggaatact tgcaagatta tcgcgacatg gttaaggcgc tggcgccgtt gcagcgccgg
      121 gacggctact ggaatgtcag ccttctcgat tcgactcatt tcggtggaaa ggaattgacg
      181 ggcactgccc tgttcaccta tggcatctcg tggggcatcc gggaaggttg gctggacggc
      241 aagacctatt tgccggttgt cgcccgcgcc tggaatgcga tggtaagcgg gtcgctccac
      301 catgacggaa tgttggggta cgttcaaggt acagcggaac ggccggcgga tggccagccg
      361 ctggattatg acaaacgacc caatgtcgaa gactttggat tgggttgttt cctgcttgcg
      421 ggaagtgagg tctaccaaat tgccgagccg ctggtcgcca atgagacgga ggcgaaggtg
      481 ccgccatatc gggagcccga tcttcacctg tcgacggcga aggagtggga ggagcggcag
      541 cggaaagagg tccacaaagc gtttgcggcg gatgtttatg ggcggatgcc ggatgtgacc
      601 atcccggtgg ggcgccggta cggggttatc gattccacgg cgctggacgg gctcgccatc
      661 aaaaagacga ttacccttga ttttctgccc ggcgatacgg ccggtgatac gaccgccagg
      721 ttgcacatgc tgttgtacct gccgaaaacg gtcaggcggg cgccggtctt ccttggctat
      781 agctttacac ccaacgacgt gacggagcat gcgccgcagt ggccattgaa ggagatcgtg
      841 cgccgcgggt atgcggtggc ggtagcatgg tactgggata tcgagccgga ccgccccgat
      901 ggctggcaga cgggaatccg taccaggctg gcgggtctcc tgcacatcga gccttatgaa
      961 tggggtgcga tcggcgcgtg ggcgtgggga atggaacgga tggcggatga gcttgaggcg
     1021 gaaacgggca tcgatccgaa acgaataatc gccatcggcc atagccggct cggaaaaacg
     1081 gcgctttggg ccggggcgaa cgatgcgcgg tttgcgatgg tgatcagcaa cgaatcgggg
     1141 gaaggcgggg cggcgatggc caggcgcgac tacggggaga cgatcgcaat gatcaatgac
     1201 cggttcccat ggtggttttc gccggcatac aaacaatttg gggctgaccc ttctgccatg
     1261 ccggtggacc agtacgaatt gctcgcactt atcgcgccgc ggcctctata tatcgccagc
     1321 gcaatgggtg atcagtggtc cgacccgcgg ggtgaattcc tgagtgcaaa gctggcggga
     1381 caggtatatc gtttgtatgg cgagaggtgc gttggcgtgg attccatgcc gcctgttgac
     1441 catcccgtgg gaagctttat cgggtatcat atccgcaccg gaaagcatga cctcacctta
     1501 tatgactggg agcggtatct tgacttcgca gccaggcggc tgaaggcggg gaaatgatgt
     1561 aaatattcct gtttttgacc taaattgggc gaaatcctgc atcgcccatg cccaagagaa
     1621 atctgatctt ccccttcatc ctggtctgca ccttgttctt cctgtgggcc tttttgcaca
     1681 atatcaacgg gatactcatt ccgaaactca aaaaggtttg cgatctgagc gacacgcaat
     1741 cctccttcat cgatgttgcc gtgtacctgg gatattttgt ctttgcgatt cccgcgggtt
     1801 tggtatccca ccggtttgga tataagaagg gcatccttac cggtttattt ctttttgcac
     1861 tcggggcgtt attgtttctt ccggcagcgg acattcgcag ttatgggctg ttcctcgtgg
     1921 ccttattcgt ggcggcaagc ggagctacct tccttgaaac cgtcgccaat ccctacgccg
     1981 ccaatatggg ttctccggag aaccccgaac agcaattgaa cttcgcgcag tccttcaatg
     2041 ggctgggcgc tatcgtagct ccccttatcg gtgagcggta tatcctctcg ggaatcgaac
     2101 actcaaaagg ggaactggcg gcaatgcagg cgcacggcca attgacagcc tatcttcagc
     2161 aggaggccaa cgccatcaag ccgccgtacc tgatcatggc ggcggtgctg gtcctggtcg
     2221 caatagcctt cctcttcacg aagcttccgg aaataaggca gaccgacggg gcgcacggaa
     2281 cggagttttc gatccgggtt ctgcgctttc cgcatgtgcg ctgggcggtg atcgccgagt
     2341 tcttctacgt cggcgcccag gtcggggtga ccagtttcct cgttcggttt tccaaatatg
     2401 cggccaatat cggcgagaag gaggcgggta aaatgctcac cctggcgatg gcgggattca
     2461 tggcggggcg gttcctgggc acgtttttta tgcgttatat ccgcccggct tcccttttgt
     2521 ccctctatgc cctgatcaat ataggattgc tgatggtcgt gttggcgggg catgggaaag
     2581 tggcggtcta cgcgctgatg accatcccgt tcttcctgtc tatcatgttt cccacgatct
     2641 ttgcactggg gatccgggac ctgggcgagg agacaaagct cgcttcttct ttcctcgtga
     2701 tgtcgatcat cggcggagcg ttcactccat tcatgatggg cctgatctcc gaccacaccg
     2761 gcggtgatat ccaggtggcc tatatcatgc cggtagtgtg tatgggattc attttatggt
     2821 ttgggcttcg gggtcatcgg gtcaggaact ttcaaccggc gctggcatga ggtattgtct
     2881 ggcattagac cttcaaaagg acgcggcgct gatcgccgaa tatgaggcct ggcatcgcga
     2941 cgtgtggcct gaaataaaaa gcagcatcc
//
LOCUS       DATGKE010000343         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_529170, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000343 DATGKE010000000
VERSION     DATGKE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529170"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VKU83_05905"
     CDS             complement(<1..427)
                     /locus_tag="VKU83_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93119.1"
                     /translation="MNILQTDIYKFSIPMHPFTIATGTMDYAQNTFIRIHTDAGYYGV
                     GECSAFPMITGETQATCFEMARDFAACWKGKSALDIPGRMQELHAFTAFNGTIKSAFD
                     MALHDLAARAAGQPLYRYLGGKKQLLETDLTIGIDTPEKM"
     gene            complement(438..2111)
                     /locus_tag="VKU83_05910"
     CDS             complement(438..2111)
                     /locus_tag="VKU83_05910"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HLI93120.1"
                     /translation="MRKYYRACTAILLFFSCRPALHFDTIIRNGSIYDGSGRPSFKGD
                     IGINADTIAFIGDLAGAKADDVLDAGGMAVSPGFIDMQSQSMVSLLQDGRSLGAIRQG
                     VTLEIFGEGSSEGPYTDSMKAMEQRQEGDIKYDVTWTTLKDYLRTLVKRGVSTNVASF
                     VGAATIRENLLGFANRAPDSAELEKMKALTREAMEDGALGIGSALIYAPGSYAKTPEL
                     IALCQVAAAYGGCYISHIRSEGNQLQQGADELIRISRDAGLPAIIYHLKAAGRSNWYK
                     MDPLIAKIDSARKAGLNISACMYTYTAGATGFDAAMPTDVQEGGLDKWIGRLKDPVVR
                     ARVIREMKTPSDRWENLYLGAGPDNIICTGFKQDSLKYLTGKRLSEIARLRHKSPEET
                     VIDLVIEDHSRVEVIYFLMSEENVKKEIRLPYMSFGSDEASMAPEGVFLKSSCHPRAY
                     GNFARLLGTYVRDEKLISLEDAIHRLTGMPAGLLHLAKRGELKVGNYADIAVFDPATV
                     RANSTYERPHQLATGVEDVFVNGAWVLKNGTHTGAKPGMVVYGPGYKAR"
BASE COUNT          466 a          631 c          659 g          554 t
ORIGIN      
        1 ccattttttc cggcgtatcg attccgatcg tgagatccgt ttcaagaagc tgctttttgc
       61 cgcccagata ccggtaaagg ggttggccag ctgcccgggc ggccaggtcg tgaagcgcca
      121 tgtcaaaggc gctttttatc gtaccattga aggccgtgaa ggcatggagc tcctgcatgc
      181 ggcctgggat atccaatgcg gatttacctt tccagcatgc cgcgaaatcc cgggccattt
      241 caaaacaggt cgcctgtgtt tcaccggtga tcatcgggaa agcagagcat tcgccaacgc
      301 cgtaatatcc agcgtccgtg tggatcctga taaaagtatt ctgcgcgtaa tccatcgttc
      361 cggtcgcaat ggtgaacggg tgcattggga tgctgaactt gtaaatatcg gtttgaagga
      421 tgttcatggc ggagtcttca tctcgctttg taacctggcc catagacgac catacctggc
      481 ttagcgcccg tgtgtgtccc attcttcagt acccaggcgc cgttgacaaa aacgtcctca
      541 acgccggtgg ccaactggtg cggccgctca tacgttgaat tggcccggac tgtggccgga
      601 tcaaaaaccg cgatatccgc gtagttgccc accttcagct ctcctctttt cgcgaggtga
      661 agaagcccgg ccggcatgcc ggtaagcctg tggatggcgt cttcgaggga aatgagtttt
      721 tcgtcccgca cgtaagtgcc caacagccgg gcgaaattgc cgtaggcgcg ggggtggcag
      781 ctgcttttca ggaaaacgcc ttccggtgcc atgctcgctt cgtcggatcc aaaactcata
      841 taaggaaggc gaatctcctt ctttacgttt tcctcactca tcaggaaata aatgacctcg
      901 acgcgggagt ggtcctcgat gaccaggtcg atgaccgttt cttccggcga cttatgccgc
      961 agcctcgcaa tctcgctgag tcgttttccg gtaaggtatt tcagcgagtc ctgcttgaat
     1021 cccgtgcaga tgatgttgtc ggggccggcg cccaggtaga ggttctccca acggtcggaa
     1081 ggcgtcttca tttccctgat gacccttgcc ctgacgacgg gatccttcaa tcgtccgatc
     1141 catttgtcga ggcctccttc ctggacatcc gtgggcatgg cggcatcgaa cccggtagcg
     1201 ccggcggtat aggtatacat gcaggccgag atgttgagtc ccgctttgcg ggcgctgtcg
     1261 atcttggcga tcagcgggtc cattttatac caattgcttc tccccgctgc tttcagatgg
     1321 taaataatgg cgggcaggcc tgcgtcgcgg gagatccgga tcagttcgtc ggccccctgt
     1381 tgcagttgat tgccttcgct gcggatatgt gaaatatagc agccgccgta tgctgcggct
     1441 acctggcaaa gagctattag ttccggtgtt tttgcgtagc ttcctggcgc ataaatgagg
     1501 gcggagccga ttccgagggc gccatcctcc atggcttcgc gggtgagagc cttcattttt
     1561 tccagttctg ccgaatctgg ggcccggttg gcgaacccaa ggagattctc tcggatggtg
     1621 gctgcgccga caaaagaggc aacgttggtg gagacgcccc ttttcacaag ggtccggaga
     1681 tagtctttga gggtcgtcca ggtgacgtca tatttgatat cgccctcctg ccgttgttcc
     1741 atcgccttca tactgtccgt ataggggccc tccgaactgc cttcgccgaa tatttcgagc
     1801 gtgacaccct ggcggatagc gcccagcgac cggccatcct ggagcaagga aaccatggac
     1861 tggctttgca tatcgatgaa gcccgggctg accgccatgc ctcccgcgtc gagaacgtcg
     1921 tccgccttgg ctcctgcgag gtcaccgata aaggcgatgg tgtccgcgtt gataccgata
     1981 tcgcccttaa aggaggggcg accgcttccg tcgtagatgg agccattgcg gatgatcgta
     2041 tcgaagtgta aagcagggcg gcaggagaaa aacaggagga tcgccgtgca agcccggtag
     2101 tattttcgca tgcacctaag gtaatcaaat tctcttaccc tacctttggc ggcaaattga
     2161 aagtctgcgc atatggatat cgcgtctgga caaatgtctt tcgagaatac ggaaaatgca
     2221 ttcgaataca aatccgacaa ggacctgaaa aaggcggcga cgctgttcgc caccatgggt
     2281 tacgctccgt tggtttatct gggaacacgg
//
LOCUS       DATGKE010000344         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_497735, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000344 DATGKE010000000
VERSION     DATGKE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497735"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VKU83_05915"
     CDS             <1..1040
                     /locus_tag="VKU83_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93121.1"
                     /translation="RDGAISRPVATAFGYHILKRISRKPFPGQWDEQTVAALKQQVMN
                     DPRVEVSRQALFARIYRETDFQRADIPEAELWAFTDTVMRNPGISSYGNLSNSTMVFS
                     YSQRAYTVKEWLDFARTTKPTRIGSALSDKELFQRYIKRTALEYYRNHLEQYNKDFSF
                     QLNEFKEGNLLFEIMQRKVWDKASADSAGLVKYYEAHKGKYWWNASADALLFTCSDAR
                     TAEDLKARLSADPAGGWRLAADSLGAAVQADSGRYELAQIPGESISAPVSRAFTQPVV
                     NKGDNTVSFAYVLTLYTERSPRSYHDARGFVINDYQGFLEEQWVASLKKEYPVKVDEQ
                     VLAGIIKKGGS"
     gene            complement(1162..2115)
                     /locus_tag="VKU83_05920"
     CDS             complement(1162..2115)
                     /locus_tag="VKU83_05920"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HLI93122.1"
                     /translation="MKVIEHLRQAKETLVSFEILPPLKGKTISSIYDHLNPLMEFKPA
                     FINVTYHRSETMFKKKTDGTFEKVEVRKRPGTVGICAAIMNRYKVDAVPHLICGGFAK
                     RETEDALIDLHFLGIDNVLVLRGDAARNESSFEPEPDGHRYAIDLLKQVVCLNQGVYL
                     EEDIKNGGRTNFCIGVAGYPEKHFEAPNLETDLMYLKAKVDAGAEYITTQMFFDNQKY
                     FDFVRVCRDQGIHVPIIPGLKPITNFKQLSMLPKVFHVDLPTDLSNAILKCKNDVEVE
                     RVGTDWLIAQSRELKKAGVPVLHYYTLGKPKVIEEVVRATF"
     gene            2328..>2545
                     /locus_tag="VKU83_05925"
     CDS             2328..>2545
                     /locus_tag="VKU83_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI93123.1"
                     /translation="MSIVIHSKDLVKTYHSRTVVNNVSIRLEQGEIVGLLGPNGAGKT
                     TTFYMVVGLIKPDSGDVFLNDVNITRQP"
BASE COUNT          575 a          704 c          714 g          552 t
ORIGIN      
        1 cgcgtgacgg cgccatcagc cggccggttg cgacggcgtt tggatatcat atcctgaaac
       61 ggatatccag aaagcctttt cccggtcaat gggacgagca gacggtcgcg gcattaaaac
      121 agcaggtgat gaacgatccc cgtgtcgaag tgagccgcca ggcattattt gcccgcatct
      181 accgggagac cgatttccag cgtgctgata ttcccgaggc ggaactttgg gcctttacgg
      241 acaccgtgat gcgaaatccc ggcatctcct catacggcaa cctcagcaat tccaccatgg
      301 tcttttccta ttcgcagcgg gcttacacgg taaaagagtg gctcgatttt gcgcgtacaa
      361 ccaaacctac aaggattggg agcgcgcttt cggacaagga gctttttcaa cggtacatca
      421 aaaggactgc gctcgaatac taccggaatc acctagagca atacaacaaa gacttttctt
      481 ttcagctgaa cgaattcaaa gaaggcaacc tgctcttcga gatcatgcag cgaaaggttt
      541 gggataaggc ttccgcggac agcgccggcc tggtgaagta ctacgaagcg cacaagggaa
      601 aatactggtg gaatgcgagc gccgacgctt tgctcttcac ctgcagcgac gccaggacgg
      661 cagaagatct taaggcaagg ctctcagctg atccggcagg cggatggcgg ctggccgccg
      721 acagtctggg cgcggcggtg caagctgatt ccggccgcta tgagctggca cagatccccg
      781 gggaatcgat atccgcaccc gtatcgcggg cctttacgca accggtcgtc aataaaggcg
      841 acaatacggt cagctttgcc tatgtgctca cgctatacac cgaacggtcg ccccgcagct
      901 accatgacgc caggggtttt gtcatcaatg actaccaggg gttccttgaa gaacagtggg
      961 tggcgtcgct gaaaaaggag tatccggtca aggtggatga gcaggtcctc gcgggaatca
     1021 tcaaaaaggg cggctcgtga cggcggcccc ctcgccggcg gctcgtgacc ggcagctccg
     1081 cgccgcgaaa tccgcacgcg aagtcctcac cgccagctct tccccctccg gccgcgtcgg
     1141 gcatcccccg tagcgggcgg gtcagaaggt cgctctgacc acttcctcga tcacttttgg
     1201 cttacccagc gtataataat gcaggaccgg gacaccggcc tttttcaact cgcggctttg
     1261 agcgatcagc cagtcggtgc cgacccgttc gacctcgacg tcatttttgc acttgaggat
     1321 cgcattggag agatcggtcg gcaggtcgac gtggaagacc ttgggcagca tggacagctg
     1381 tttgaaattg gtgataggct tcagcccggg aatgatgggc acatgaatgc cctggtcgcg
     1441 gcagacgcgg acaaaatcga aatacttctg attgtcaaag aacatctgcg tcgtgatata
     1501 ttcagctccc gcgtcgactt tcgccttgag atacatcagg tcggtctcga ggttgggcgc
     1561 ctcgaaatgt ttctcgggat agccggctac gccgatacag aaattggtcc ggccgccgtt
     1621 cttgatgtct tcttcaagat agacgccctg gttgaggcaa accacctgtt tgagcaggtc
     1681 gatggcgtac cggtgcccgt cgggttccgg ttcgaaggag ctttcgttcc ttgctgcgtc
     1741 gccgcgaagg acgaggacat tatcgatgcc aaggaaatgc aggtcgatga gggcatcttc
     1801 ggtttcccgt tttgcgaatc cgccgcagat aaggtggggt acggcatcca ccttgtaccg
     1861 gttcataatg gcggcacaga tgccaacggt gccggggcgt ttgcggacct ccaccttttc
     1921 gaacgtgccg tcggtcttct tcttgaacat ggtctcactg cgatggtaag tcacattgat
     1981 gaaagccggc ttgaattcca tcagggggtt caggtgatcg tagatggaac tgatggtctt
     2041 ccccttgagg ggcggaagaa tttcgaatga aaccaacgtc tcttttgcct gccgcagatg
     2101 ctcgatgacc ttcataagtt ccgcaaaata gtcgatttga atgtaacggc aaaccgggcg
     2161 ctgaccatcc ggtcaaaaca cccggaatcg caaaccccgt tcggagcaaa ttactcttaa
     2221 aacacctata attttggctc cggacacttg cggcatgccg cacagggcgt aaatttgcgt
     2281 ccaaaaccgg gcgccgcccg cttcccaccc attcaaaaat tgaagatttg agcattgtca
     2341 ttcattccaa ggacctcgtg aagacgtatc attcccggac ggtggtgaac aacgtctcca
     2401 tcagattgga gcagggcgag atcgtcggac ttcttggtcc gaacggcgcc ggaaagacca
     2461 cgaccttcta catggtcgtc ggcctgatca aaccggatag cggcgatgta ttcctgaatg
     2521 atgtcaatat tacgcgccaa cccat
//
LOCUS       DATGKE010000345         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_775239, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000345 DATGKE010000000
VERSION     DATGKE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775239"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VKU83_05930"
     CDS             complement(<1..625)
                     /locus_tag="VKU83_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLI93124.1"
                     /translation="MAIFNSSNPTLSEKIFTKSLAQPGLGTMTVRGSIQKFGFLMLLV
                     LAGAAYTWKLYYSFKVPTMMGFMMVGVFGGMICAFATIFVPKLAKYLSPAYALLEGFA
                     LGGISAFVNDQFKVKYPNIVMEAVLLTFGVAFAVFLLYNFRVIRATNKFRAVILSSMI
                     GIAIFYFIDIILGFFGVTIPFMQFGDNSVIGIVVNLFVVVIAALSLVL"
     gene            complement(650..1945)
                     /gene="ricT"
                     /locus_tag="VKU83_05935"
     CDS             complement(650..1945)
                     /gene="ricT"
                     /locus_tag="VKU83_05935"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HLI93125.1"
                     /translation="MGCSNCGTSKGGKPAGCKSNGGCSTGGCNRLNVYDWLANLPFSD
                     PESTCRIIEVSFNQGSRKEFYRNSTVQALCKGDLVSVEGVSGFDVGEVTMTGEMVRLQ
                     LKKKGIEEKNPDLKKVLRRASEKDVEMMQQNKSREKEALVRSRAIAPRLKLEMKMSEV
                     EIQADGRKATFFYIADDRVDFRELIKLYAGEFRLKVEMRQIGARQEAAKVGGVGSCGR
                     ELCCATWLSDFKSVNTTAARYQNLSINQSKLSGQCGRLKCCLNYELDTYLDALQNFPD
                     NAETLQVAKGTASLIKKDIFKNLMWYVLPDSNKQYPLTIERVRKIRQMNMQNTIPETL
                     DHVEVSTGKPKEPETGFVDLVGQISLKTLEKADRKKKHVHHKHHSQHASGGSGNRAGE
                     PQNAGGNKGSQPDGRRGRDGNPAGKKPAGPPPPDKNKKR"
BASE COUNT          487 a          613 c          524 g          546 t
ORIGIN      
        1 taagcaccag gctcagagcc gctatcacta ccacgaagag gttgacgaca atgccaatga
       61 cgctattgtc accgaactgc atgaaaggaa tagtgacgcc gaaaaacccc aggatgatgt
      121 cgataaaata aaagatcgcg atcccgatca tcgaggaaag gatcaccgcc ctgaatttgt
      181 tcgtcgcccg gatgacccgg aaattataca gcaggaatac cgcaaaggcc accccaaagg
      241 tgagcagcac ggcctccatc acgatattgg ggtatttcac cttgaattga tcattgacga
      301 acgccgatat cccgcctaaa gcgaaccctt ccaatagggc ataggcaggg gacagatatt
      361 tagcgagctt cggaacaaag atcgtagcaa aagcgcagat catgccgccg aacacgccga
      421 ccatcatgaa tcccatcatc gtagggacct tgaacgaata gtacaatttc caggtatacg
      481 cggcgcctgc cagtaccagg agcatcagga atccgaattt ttggatggat cctctaaccg
      541 tcatcgttcc cagacccggt tgggcgaggg acttggtgaa gatcttttcc gacagggtag
      601 gattactgga attaaagatt gccatagtta tagagtttat gcaaagttat cacctttttt
      661 tattcttgtc cggcggcggc ggtccggcgg gttttttacc ggcggggtta ccgtccctgc
      721 cgcgacgacc gtcaggctgg ctgcccttat tgcccccggc attctgcggc tcgcccgctc
      781 tgttgccgct cccacctgac gcatgctgtg aatgatgttt atggtggacg tgtttctttt
      841 tacggtcggc cttttcgagg gtcttcagac tgatctggcc gaccagatct acgaacccgg
      901 tctccggctc tttgggtttg cccgtgctga cctcgacatg gtcaagggtc tcagggatag
      961 tattctgcat gttcatctgc cggatcttcc ggacccgctc gatggtaagg ggatactgct
     1021 tattactgtc cggaagtaca taccacatca ggttcttgaa aatatccttt ttgatgaggc
     1081 ttgctgttcc tttggctacc tgcaaggtct ccgcgttgtc agggaagttc tgcaaggcat
     1141 ccaggtaggt atccagctcg tagttaagac aacattttag ccggccgcac tgaccgctca
     1201 gcttgctttg gttgatggag aggttttgat acctcgccgc ggtcgtattc acggatttga
     1261 aatcgctgag ccaggtggcg caacacagtt cgcggccgca gctacctacg ccgccgacct
     1321 tcgccgcttc ctggcgggct ccgatctgcc gcatttccac ctttagccga aactcaccgg
     1381 cgtatagctt gatcagttcc cggaagtcta cccggtcgtc cgcgatatag aaaaaggtgg
     1441 cttttctgcc atcggcctgg atctctactt cgctcatctt catctccagt ttcagccggg
     1501 gggctatcgc ccggctccgg accagggcct ctttctcgcg ggacttgttc tgctgcatca
     1561 tttccacgtc cttttcgctt gcccggcgta acaccttttt gagatccgga ttcttttctt
     1621 cgatcccttt cttttttagc tgcagccgta ccatctcacc cgtcatcgtc acttcaccga
     1681 catcgaaacc gctgacccct tcaacagaaa caaggtcccc cttgcaaagg gcctgcaccg
     1741 tagaattccg gtagaactct ttccggcttc cctggttaaa gctaacctcg atgatccggc
     1801 aagtgctttc aggatcgcta aaaggaagat tggccagcca gtcatataca ttcaaccggt
     1861 tacagccgcc ggtgctgcac ccgccgttgc ttttacaacc ggccggtttg ccgcctttgg
     1921 atgtaccgca attactacaa cccatagctt acaaatttat tcccttcaat tacaagtaat
     1981 taaaaccttt ttctgttttg taaaaagaag gagccgcgca caggcaggaa gacgctaccg
     2041 gcagggaaag ctaacgcgtt acacgaaata tattgagatc aaatacgctt ttccaacgga
     2101 accggcatct gccgcacata ttcccgcaca ctgcctggcc cggaaacctc tttttactgg
     2161 cctttaacgg
//
LOCUS       DATGKE010000346         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_338461, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000346 DATGKE010000000
VERSION     DATGKE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338461"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VKU83_05940"
     CDS             <1..395
                     /locus_tag="VKU83_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93126.1"
                     /translation="LFLFIEDSLLHLDAAEGRVLANFALFFTLHLAGFFGFRIQDAYS
                     ERNPILDLREGRFVPEMPQHGQALEAALAFHTAQLLRVQRPEELLELPLNQETRRQLL
                     QAYQTFYSIQIPEFGEMKTLGVLQTVLS"
     gene            complement(392..1564)
                     /locus_tag="VKU83_05945"
     CDS             complement(392..1564)
                     /locus_tag="VKU83_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI93127.1"
                     /translation="MNYRKFRADKLFDGHAFVPFDAVLIASPDGIVQAVVPAGEAGEG
                     IESYRGVLCPGFVNCHCHLELSHLRGVVPERTGLVDFLSAVIGQRGKFKPAEIAAGIL
                     KAEQEMLDNGIVAIGDICNSTDSLERKKGGRLTCYNFIETMGFIERTSPDRFAAAVRV
                     YEAFEKHFPGKNSVVPHAPYSVSPALFQLVAGFAGNRIISMHSQESVAENEFYLTGGG
                     DLLRLYRNLGLDLSFFRGTGRRSLASVMEFFDPAQRMILVHNVDLAEADLGGLRGRPN
                     LYFCLCPNANLYIGGQLPAIEMLVSRGCRMVVGTDSLASNHGLNILAELCTLQEAFPR
                     LATETLLGWATAGGAEALGMDGMLGSFSSGLRPGVLLIEGLAGDRFTASTAPRRLL"
     gene            1638..>2244
                     /locus_tag="VKU83_05950"
     CDS             1638..>2244
                     /locus_tag="VKU83_05950"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HLI93128.1"
                     /translation="MLDKLEAIKARFEDLGIALSNPEIVSDNRKFSAMSKEYRSIEKI
                     VRVYEEYKEVLDTIAFNKEALKEDDPELRELAKAEAPGLEEKRQQLEAQIRQLLIPKD
                     PQDEKNAILEIRAGTGGDEASLFAGDLLRMYLRYCERRGWKTAILSESEGTVGGYKEV
                     QVEVVGEDVYGTLKFESGVHRVQRVPDTETSGRVHTSAATVA"
BASE COUNT          528 a          679 c          624 g          413 t
ORIGIN      
        1 aattgttcct gttcatcgag gattcgctgc tccaccttga cgccgctgaa ggccgcgtgc
       61 tcgccaattt cgcattattt ttcacccttc acctggccgg gttcttcggc tttcgaatcc
      121 aggatgccta cagcgaacgg aacccgatcc tggacctgcg ggaaggccgg ttcgtaccgg
      181 aaatgccgca gcacggccag gcgctggaag ctgcgctggc ctttcatacg gcccaactgt
      241 tgcgggtcca gcggccggaa gaattgctgg agctgccgct caaccaggag acgaggaggc
      301 agctgctcca ggcctaccaa accttctatt cgatccaaat accggagttc ggtgagatga
      361 agacgctcgg ggtccttcaa accgtcctgt cctaaagaag ccttcttggc gcagtggaag
      421 ctgtgaaccg gtcacccgcc aatccttcga tcagcaggac gccaggccgt aacccggacg
      481 aaaaggatcc aagcatgcca tccatcccca gcgcttccgc cccgcccgcg gtcgcccagc
      541 ccaaaagcgt ttccgtcgca aggcgcggaa aagcctcctg cagggtacag agctcggcga
      601 ggatattcaa cccgtggttc gaggccagac tgtccgttcc caccaccata cggcagccgc
      661 gggaaacaag catctcaata gccggtaatt gcccgccgat gtagagattc gcattcggac
      721 acaaacagaa gtaaaggttt ggccggcctc ttaggcctcc aaggtccgct tcggccagat
      781 cgacattatg cacgaggatc atgcgctgtg ccggatcaaa aaattccatt acgctcgcca
      841 ggcttcgcct gccggtgccc cgaaaaaagg acaggtccag ccccaaattc cggtaaagcc
      901 gcagcaggtc acccccgccg gtcaaataaa actcattttc cgcaacgctt tcctgggaat
      961 gcatcgagat gatcctgttc ccggcgaacc ctgcaaccag ttggaacaaa gcaggcgaaa
     1021 cggaatacgg cgcatggggc acaaccgaat ttttgccggg gaaatgcttt tcaaacgcct
     1081 catatacccg caccgcggct gcaaaacggt cgggggaagt cctttcgatg aaacccatcg
     1141 tttcgataaa gttgtaacag gtcagtcgcc cccctttttt tcgttccagg ctgtccgtcg
     1201 aattacagat gtccccgatg gcaacgatgc cgttgtccag catttcctgc tcggccttaa
     1261 ggatgccagc cgcgatctcg gccggcttga atttgccccg ctgcccgatg accgcgctaa
     1321 gaaaatcaac cagccccgtt cgctccggga ccactcccct caggtgcgat aattcgaggt
     1381 ggcaatgaca attgacaaag cccgggcata aaacgccccg gtaggactcg atcccttctc
     1441 cagcctcccc ggcggggacg accgcctgca cgatgccgtc aggactggct atgagcaccg
     1501 cgtcgaacgg aacgaaagca tggccgtcga acaatttatc cgccctgaat ttccgataat
     1561 tcatcgcctc aaattttatc tttttatctt tgcgctcgcc cgtgccgcgg accaggcgcg
     1621 tcaagaatat cgaagatatg ttagataagt tagaagccat taaggccagg ttcgaggacc
     1681 tgggaatcgc cctgagcaac ccggaaatcg tcagcgacaa ccggaagttc agcgcgatga
     1741 gcaaggagta tcggagcatc gaaaagatcg tacgggttta cgaggaatac aaggaagtcc
     1801 tcgataccat agcgttcaat aaggaagccc tgaaggagga cgaccccgaa ctccgggagc
     1861 tggcaaaggc ggaagcgccg ggactcgaag agaagcggca gcaactcgaa gcccagatcc
     1921 gccagctgct gatcccgaag gacccgcagg acgagaaaaa tgccatcctc gagatccgcg
     1981 ccggaacggg cggcgatgaa gccagcctgt ttgccggcga cctcctgcgg atgtacctgc
     2041 gctattgtga acgacgcggc tggaaaaccg ccattttgag cgagagcgag ggtaccgtcg
     2101 gcggctataa ggaagtacag gtggaagtgg tgggggagga cgtttacggg acgctaaaat
     2161 tcgagagcgg cgtccaccgg gtccaacgcg tcccggatac cgagacaagc ggccgggtcc
     2221 ataccagcgc cgccaccgtg gccg
//
LOCUS       DATGKE010000347         6298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1432612, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000347 DATGKE010000000
VERSION     DATGKE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432612"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VKU83_05955"
     CDS             complement(<1..427)
                     /locus_tag="VKU83_05955"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLI93129.1"
                     /translation="MWIKLSKKTVRAVTLIAAALPAVLTCKAQRTEKPPLHGNHWMAV
                     TGRPLAAEAGAQIFQKGGNAIDAACAMLAAVCTMHIVLTWGGETQALIYNPKTGKVIA
                     INALGWAPTGATPAFFKSKGYNFPPEYGPLAAVTPGTPGG"
     gene            complement(411..1421)
                     /locus_tag="VKU83_05960"
     CDS             complement(411..1421)
                     /locus_tag="VKU83_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HLI93130.1"
                     /translation="MRLFIIFLAVAALAGCQEAAPTGPTLAEYFAQQDSGVQTGNVKM
                     VEISTPKGKFHVWTKRFGYNPKIKVLLLHGGPGGTHEFFESFENFLPKQGIEFIYYDQ
                     LESLNSDHPNDSSLWTPEHFVEEVEQVRQALALNKENFYLLGHSWGGILAMEYALKYQ
                     DNLKGLIISNMMASMPEYEKYNGVLRSKMRKSLVDSLQAYEAKGQYDDPVYEKLMINE
                     YYNKHLCRVVPWPEPVVRGFNHLSKGIYVSMQGPSEFKTGGRLIKWDRSKDLGRIRVP
                     TLMIGAQFDTMDPNYMEWMSKQVQHGSYLYCPEGSHCDMWDDQQDYFPGLIKFLKDVD
                     KT"
     gene            1547..2653
                     /locus_tag="VKU83_05965"
     CDS             1547..2653
                     /locus_tag="VKU83_05965"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLI93131.1"
                     /translation="MQKFACLLAVVLLIAITPSTGQQSAAKVYPNQTEGDFAISHFPF
                     RSGETTLPVLKIHYITLGQPVKDKKGKVTNAVLIMHGTTGSGANFMSDYFAGNLFGRG
                     QLLDASKYFIVLPDGIGHGHSSKPSDALHMRFPHYTYDDMVLADYDLLTEHLGVNHLR
                     LVMGTSMGAMHSWIWGYTYPDFMDALMPLASLPVQIAGRNRMMRKMAIEAIESDPAWE
                     KGEYTRVPETGMRGAYASLFFMTSSPWQLQKHAPARADADQLLDRGIAAFLKRQDPND
                     FIYAFDASRDYDPSPHLAKIKAPLFAVNSADDEVNPPELGIMEKEIRKVARGRYILIP
                     WSEKTSGHGTHSNPTIWGDYLKELLAVSAPGRRE"
     gene            complement(2677..4278)
                     /locus_tag="VKU83_05970"
     CDS             complement(2677..4278)
                     /locus_tag="VKU83_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93132.1"
                     /translation="MRTRVMAGLGVFILMTGSRLGAQEVTFCEPYSDRFTVNEELVAK
                     IGNYFWVEMTSRQRPTRHSADPGEDRLFVIYNNRMKAINEVNHIACPGTEIKEYLIAD
                     SDHFDQLFLAENANRHVDLWVQRYTPDGMAVGSERKVGEMPFFEPGNSFLLVRSADRN
                     LALLLGFEFMPGGAPKIHAMLFNTDWGILSSMVYNHPFLTQPMIQDDFSGYPMEDFDQ
                     GTVKLANDGDWLMVSPSRTNNNYLLSHFTGTDTGFRYEEIWLPKIAATEDISLSIDNK
                     TNEAIAGVLSDFHYAPLKNVRIVHYSMTYKQYDFDTTYRLTTLGGGRVRNSNLVKENF
                     MAMPGGGFLLLKEYGRLFEEPIDDRSIDDGWDPALMFAENNIPDPDEGPSAGHRVKVP
                     APHYGYARYSAPMNIIYHDRGDLSLYYFPSMRGDSTWSGMISEEQITELNSPNLSYMI
                     VPFGDKLFCLYNSFVHSEQLYAASTVLNRKGQLVTDQGVLFWGIKTPLDFQRSRQISP
                     HEVVVPYQRLGKPGFVVVDLAAPAG"
     gene            4692..>6298
                     /locus_tag="VKU83_05975"
     CDS             4692..>6298
                     /locus_tag="VKU83_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HLI93133.1"
                     /translation="MRTPISVILLSCLTLTGHTTPNIRTGTIPSWVTPVVPDRNRQPF
                     PKDISNGYYYELQDQQTDLLLQTEYFHSIRHLVNRSGVQDGSEVSVTFAPQYQEVVFH
                     RIDVIRDGGIIDELRSGHLHVAEEEPDAANFQYTGKKRAYFTLPDIREGDRIEIAYSV
                     IGFNPVFHGKYATQQYFTRSTAVCNYFRTIITSRGRPLYFLAHNDALLPGRELAGSRL
                     IYRWSNPVLAEDSNPAAGEARVPYVGITEYKDWREVAGWALNTFDHYRAPLPLRLVEK
                     ISSWRQIAAGNPDLFANLATRFVQNEIRYLAIETGANSHRPHSPAEVLSRGYGDCKDK
                     ALLLVSILRHEGIPAVAALISAEGGRSLPGSAPSPGLFNHVIVAIRRPGDRWLYVDPT
                     LAAQRGELSELYIPDYGYALLVRDGEDSLRTIKPGQASYTILEQLDARFDDTSRLLVT
                     TTFAGGAADQLRRSFDENSRKDIEAAYVKYYAAWIPGIKMTASLRSDDDSVANRITTR
                     ESYAVPTIWGLDREGRRSFVYLVKMVR"
BASE COUNT         1388 a         1887 c         1582 g         1441 t
ORIGIN      
        1 gcccgcctgg cgtgcccggc gtcaccgccg ccagtggacc atattccggc gggaaattat
       61 accccttgct tttgaaaaat gccggcgtcg ctccggttgg cgcccaacca agcgcattga
      121 tcgcaattac ttttccggtc ttgggattgt agatcagcgc ctgtgtctct ccaccccagg
      181 tcagcacgat gtgcatcgtg cacaccgcag ccagcatggc acaggccgca tcgatcgcat
      241 tccccccttt ctggaagatc tgcgccccgg cctccgccgc caacggtctg ccggttaccg
      301 ccatccaatg atttccatgc aacggcggtt tttccgtccg ctgcgctttg caggtcagaa
      361 cggcagggag cgccgccgca atcaatgtaa ccgcccgcac cgtcttttta ctaagtttta
      421 tccacatctt tcaaaaattt tatcaatccc ggaaaatagt cttgctgatc gtcccacata
      481 tcgcagtggc tgccctcggg acaatacaaa tacgacccat gctgcacctg cttgctcatc
      541 cattccatat aattcgggtc catcgtgtca aattgcgcac cgatcattag ggtcggcacc
      601 cggatcctgc cgagatcctt cgaccggtcc cacttgatca gccttccccc cgtcttgaac
      661 tcgcttggtc cctgcatgct gacatagatg cccttgctca ggtgattgaa accccgcact
      721 accggttcag gccagggcac tacgcggcaa agatgcttat tgtaatattc attgatcatc
      781 aatttctcat aaacgggatc gtcgtattgg ccctttgcct cgtatgcctg cagcgaatcc
      841 accagggatt tacgcatttt cgaccgcaat actccattgt atttttcata ctcgggcata
      901 ctggccatca tatttgagat aatcagaccc ttgaggttgt cctggtattt caacgcatat
      961 tccattgcca ggatccctcc ccaggaatgt ccgagcaggt aaaaattttc cttattgagc
     1021 gccagggcct gccgcacctg ctccacctcc tccacaaaat gctccggcgt ccagaggctg
     1081 ctgtcgttcg ggtgatcgct gttcaatgac tccagctgat cgtaataaat gaattctatt
     1141 ccctgctttg gcaagaagtt ctcaaatgac tcaaagaact catgcgtgcc gcccggtccg
     1201 ccgtgtagca gcagtacctt gatctttgga ttataaccaa accgttttgt ccagacgtgg
     1261 aattttcctt tgggcgtcga tatttccacc attttcacgt tcccggtctg aacgcccgaa
     1321 tcctgctgcg cgaagtattc ggccaaagtg gggccggttg gcgctgcctc ctggcaaccg
     1381 gcgagggccg caaccgcaag aaagatgata aacagtctca taaacattct gttttgcaac
     1441 ctaaaagata cggcgctttc ttccgcgggg taactcactt tttgcgtagt gcccgcggcc
     1501 gatcgatcgt ttaataaatt cactcccgaa ccaataacga agtttcatgc aaaaatttgc
     1561 ttgtctcctg gccgtcgtat tactgatcgc gataacgcct tcgaccggtc aacagtccgc
     1621 cgccaaggtc taccctaatc aaacggaagg agactttgcg atcagtcatt tccccttccg
     1681 gagcggcgaa acgacgcttc ccgtattgaa gatccactat atcacgcttg gccagccggt
     1741 aaaagacaag aaaggcaagg tcaccaatgc cgtcctcatc atgcacggca caaccggcag
     1801 cggcgctaat ttcatgagtg actatttcgc cggaaacctg ttcggccggg gccagctcct
     1861 cgatgcatcg aaatatttta tcgtcctgcc tgacggcatc gggcatggcc attccagcaa
     1921 acccagcgac gctctgcaca tgcgcttccc gcactatacc tatgacgata tggtactcgc
     1981 ggattacgat ctgttaaccg aacacctggg ggtcaaccat ctcaggctcg tcatgggcac
     2041 atccatgggc gcaatgcatt cctggatctg gggctacacc tatccggatt ttatggatgc
     2101 cctcatgccc ctcgccagcc tgcccgtcca gatcgccggc cgcaaccgga tgatgcgtaa
     2161 aatggctatc gaggccattg aatcggatcc cgcgtgggaa aaaggcgaat atacccgcgt
     2221 tcccgaaacc ggcatgcgcg gcgcgtatgc atcgcttttt ttcatgacca gcagcccctg
     2281 gcagttacaa aaacacgctc ctgcccgcgc tgacgccgac cagctccttg acagaggcat
     2341 tgctgccttc ctgaaacgcc aggaccccaa cgacttcatt tatgctttcg acgcatcgcg
     2401 cgactatgac ccttcacccc atctcgctaa gataaaggcc cccttgttcg cggtcaattc
     2461 cgctgacgac gaagtaaatc cgcccgagtt gggcattatg gaaaaagaaa tcaggaaggt
     2521 cgcaagaggc cggtacatcc tgattccctg gtcggagaag acttccggcc acggcaccca
     2581 ttccaatccg acgatctggg gcgactacct gaaggaattg ctcgcggtca gtgcaccggg
     2641 ccgccgggag taaccggctg cctggggcgg tctgattcag cctgccggcg cggcaagatc
     2701 caccacaaca aatcccggct tcccaagccg ctggtaaggc accaccactt cgtgcggcga
     2761 tatctggcgc gaccgctgaa agtccagcgg cgtcttgatg ccccagaaaa gaacgccctg
     2821 gtccgtcacc agctgcccct tccggttgag gacagtcgag gcagcataaa gttgttcgct
     2881 atgtacaaaa ctattataca ggcagaacaa tttgtcccca aacggcacga tcatgtaaga
     2941 caggttcggc gaattcaatt ctgtgatctg ctcctcgctg atcatcccgc tccacgtgct
     3001 gtcgccccgc atcgacggaa agtaatatag actcagatcg ccgcggtcat ggtatatgat
     3061 gttcatgggg gcggaatacc gcgcataccc gtagtgcggt gccgggactt taacccggtg
     3121 acccgctgag gggccttcat ccgggtccgg aatgttattc tcggcaaaca tgagcgccgg
     3181 atcccaaccg tcatcgatcg acctgtcatc tatcggctct tcgaacagcc ggccatattc
     3241 tttcaataac aggaacccgc cacccggcat cgccatgaag ttctccttca cgaggttgct
     3301 gttgcgcact cttccgccgc cgagggtggt aagcctgtac gtggtgtcga aatcatattg
     3361 cttataggtc atcgagtaat gcacgatgcg cacattcttc aggggagcgt aatggaaatc
     3421 ggacaatacc cccgcgatcg cctcgttcgt cttgttatcg atcgacaggc tgatgtcttc
     3481 ggtcgccgcg atcttcggca accagatctc ttcatagcgg aaaccggtgt ccgttccggt
     3541 gaaatgactc aataaataat tgttgttggt ccgggaaggc gataccatca gccagtcgcc
     3601 atcgttagcc agcttgaccg ttccctgatc gaaatcctcc attgggtatc cgctgaaatc
     3661 gtcctggatc atcggctgcg tcagaaaagg gtgattgtat accatcgagg agagtatccc
     3721 ccagtccgta ttaaaaagca tggcatggat cttcggcgcc cctccgggca tgaattcaaa
     3781 accgagcagc aaggccaggt tcctgtccgc cgaccgcacc aacagaaagc tattgcccgg
     3841 ctcaaaaaac ggcatctcgc ccaccttccg ctcgctgcct accgccatcc cgtcgggggt
     3901 atagcgctgc acccaaaggt ccacatggcg gttggcattt tcggccagga atagctgatc
     3961 gaagtgatcc gaatcggcga tgaggtattc ctttatctct gtgccggggc aggcgatatg
     4021 gtttacctcg ttgatcgcct tcatccggtt attatagatg acgaacaagc ggtcttcgcc
     4081 gggatcagca gaatgtctcg tgggccgctg cctgctggtc atttccaccc aaaaataatt
     4141 gccgattttt gcgaccagct cctcattaac ggtaaagcgg tcgctgtaag gttcacaaaa
     4201 ggtgacctcc tgggccccga gccgggaccc ggtcatgagg ataaagaccc ccaagccagc
     4261 cataaccctc gttctcatag aacagccccc tcgcgttttg acatgtttta gaaagtaatt
     4321 aacgatattt tcgcctctcc ggtttaccgc gggtatcaaa ttccacaatg cccttaaaat
     4381 accacagtta aaaaacggtt ttggcccacc cgggcaagga ctcttccagc catgggaaaa
     4441 aaattccggc gctgggacga aaatcgtaaa tggggcattc cagaactggg aaccgccgct
     4501 gcagccgggg gtcaggtatc tggcagtcaa tcttttacaa cataggttga gtttttggct
     4561 tttgcgggcc tcttccggca cgcccattgc cttatatact tcggtttttc ataggatatt
     4621 ggatttaaaa acggggctgg atttctatcc tggccctttt ttttttaact ttatggagct
     4681 aaaatccgtc catgcgaacc ccgatatccg ttatcctatt gtcatgcctg accctgaccg
     4741 gccatactac tccgaatatc cgaaccggca ccattccgtc gtgggtcaca cccgtagttc
     4801 ccgacaggaa ccgccagcct ttcccgaaag acatcagcaa tggctattat tatgagctgc
     4861 aggaccagca gacggacctg ctgctgcaga cggaatattt ccatagcatc cgccatctgg
     4921 tcaaccgctc cggggtccag gacgggtccg aagtgtcggt gacctttgcg ccgcaatacc
     4981 aggaggtcgt tttccaccgc atcgacgtca tccgtgacgg cggcatcatt gacgagctcc
     5041 gttccggcca tttgcatgtt gcggaggaag aaccggatgc cgccaacttc caatacaccg
     5101 gaaagaaaag agcctatttc acgcttccgg atatcaggga aggggatcgg atcgaaatcg
     5161 cgtattccgt cattggtttc aatcccgtct ttcacggcaa atacgcgacg caacaatatt
     5221 tcacgcggtc aacggcggtc tgcaattatt tccggaccat catcaccagc cgcggccggc
     5281 cgctgtactt tctcgcgcat aacgacgcgc tactgcccgg ccgggaactg gcgggaagcc
     5341 ggttaatcta ccgctggagc aatccggttc ttgcggagga ctccaatccg gccgccgggg
     5401 aagccagggt gccttacgtc ggaataaccg aatacaaaga ctggcgggag gtcgccggct
     5461 gggcattgaa taccttcgac cattatcgtg ctcccctccc gctccggctg gtggaaaaga
     5521 tcagctcctg gcgacagatc gctgccggta accccgacct ttttgcaaac ctggccaccc
     5581 ggttcgtgca aaacgagatc cggtatctcg ccatcgaaac gggtgcaaac agccaccgcc
     5641 cgcattcgcc cgccgaggtc ctttcgcgcg gctatggcga ttgcaaggat aaggcactgc
     5701 tattggtcag tatccttcgt cacgagggca ttccggctgt tgcagccctg atcagcgccg
     5761 aaggcggcag gagcctgcct gggtctgctc cttctccggg cctgttcaat catgtgatcg
     5821 tggccattcg gcggcccggc gaccgatggc tttacgtgga ccctacgctg gcggcgcaaa
     5881 ggggcgagtt atcggaactg tatatacccg attatggcta cgccttattg gtgcgcgacg
     5941 gtgaagacag cctccgcacc atcaagccgg gtcaggccag ctacactatc cttgaacagc
     6001 tcgatgcccg gtttgatgac acgtcccggt tgctggtcac caccaccttc gccggcggtg
     6061 cggccgatca gctcaggcga tccttcgacg aaaacagccg gaaggatatc gaagccgcat
     6121 atgtcaaata ttacgctgca tggattcccg gaatcaaaat gaccgcgtcc ctccggtccg
     6181 acgatgacag cgtggccaat aggattacga cgcgggagtc ctatgctgtc ccgacaatct
     6241 ggggcttgga cagggaaggc cggcgttcct tcgtttacct cgtcaaaatg gtccggga
//
LOCUS       DATGKE010000348         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_940794, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000348 DATGKE010000000
VERSION     DATGKE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940794"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VKU83_05980"
     CDS             <1..375
                     /locus_tag="VKU83_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93134.1"
                     /translation="FENDRQVEIIQGDSGAILKGILRDIKEPVLFWLDGHYSSEFFVG
                     EELIKTARTDKDTPVVEELDTILGSPLNHVILIDDARGFDGLGDYPSIASIKRKVRKA
                     KGPAYSCKVENDIIQIFPKTGQ"
     gene            382..1383
                     /locus_tag="VKU83_05985"
     CDS             382..1383
                     /locus_tag="VKU83_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HLI93135.1"
                     /translation="MKRITVKFVDFWTDMNKPEGNYFYELLSQRYIVEFSDDPDIVFY
                     SCFGKEYLKYRCLRIFYSPENWRPDFTGCDFAMTFDYNEDPRHFRLPLWALYYIGYVN
                     QQGFPELDDDVNEEELVKRWKGKTKFCCFIVSNPSAKKRIEFFQKLNKVKRVDSAGRH
                     LNNIGYFLEGGTFVKMDFIKDYRFVISFENCSSVGYTTEKLIEPLLIGAIPIYWGNPA
                     VGKEFNKKRILYYDDFPSEQALIERIMEIENDPTQALKILKEKAFAGEKFNISRTGND
                     LLNFLVASIESMAGRKPVALRPLKSMRHSFKMHRSRVINRLALLRRLEMTAQKLKLS"
     gene            1385..2164
                     /gene="rfbF"
                     /locus_tag="VKU83_05990"
     CDS             1385..2164
                     /gene="rfbF"
                     /locus_tag="VKU83_05990"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HLI93136.1"
                     /translation="MKVVIFAGGMGTRISEESHLKPKPMIEIGDKPILWHIMKIFSSQ
                     GFNDFIICLGYKGYVIKEYFLNYFLHNSDVTIELKDNKVSVHNTKAEDIKVTLVETGL
                     QTRTAGRLRRVRQHLQGEDFMLTYGDGLADIDLRSLVAFHKSHGKIASVTAIQPLGRF
                     GVMQINSDNSVDVFKEKVKEDEGWINGGFFVLKPAVFDYLPVNADELMWEDLPIQNLA
                     KDDQLMAYQHHGFWKCMDALRDKIELEELWNSANAKWKIWQ"
     gene            2161..>2672
                     /locus_tag="VKU83_05995"
     CDS             2161..>2672
                     /locus_tag="VKU83_05995"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLI93137.1"
                     /translation="MKTLFGGVYSDRKVLVTGHSGFKGSWLLLWLKHLGSNVTGFSLA
                     PSTAPSHIELLGFFDPSSAGDIRNMDQLIKAFTDFQPEIVFHLAAQPLVRRSYADPLE
                     TFTTNITGTANVLEAARHTPSVKAFVNVTTDKVYRNNDWPRPYRETDMIGGHDPYSTS
                     KACVELLHES"
BASE COUNT          744 a          582 c          698 g          648 t
ORIGIN      
        1 tttgaaaacg accggcaggt ggaaatcatc cagggggata gcggcgccat tctgaaaggg
       61 attttgagag atatcaagga gccggtcctg ttttggctgg acgggcacta ctcttctgaa
      121 ttttttgtgg gcgaggagct gatcaaaacg gcaaggaccg ataaggatac ccccgtggta
      181 gaagaattgg acaccatcct tggttctcct ctcaatcatg tgatcctgat agacgacgcc
      241 cggggcttcg acgggttagg cgactatccc agtatcgcca gcattaagcg caaagttcgg
      301 aaagccaaag gcccagccta cagctgcaag gttgaaaacg atatcattca gatattcccc
      361 aagacgggtc aataggcaag tgtgaaaaga attacggtta agttcgttga tttctggacg
      421 gatatgaaca agccggaggg caactatttc tatgagctgc tctcccagcg gtacattgtc
      481 gaattttccg atgacccaga tatcgtgttt tattcctgct tcggtaagga atacctgaag
      541 tacaggtgtc tgcgcatttt ttatagcccg gagaactggc gacccgattt caccggttgc
      601 gattttgcga tgacatttga ttacaacgaa gacccgcgac acttcaggtt acccctctgg
      661 gctttgtatt atatagggta tgtcaaccag caaggctttc cggaactgga cgacgacgtg
      721 aatgaagagg aactcgttaa acggtggaag ggtaaaacca aattttgctg tttcattgtg
      781 tcaaacccct ccgcgaaaaa acgaattgaa ttttttcaaa aactcaataa ggtgaagcgg
      841 gtggattccg cggggaggca tttgaacaat atcggttatt tcctggaggg gggcactttt
      901 gtaaaaatgg attttattaa agactaccgg tttgtcatct ccttcgagaa ctgttcgagt
      961 gtgggctata ccactgaaaa attgatcgag cccttgttga tcggggccat cccgatttat
     1021 tggggaaacc ctgctgtcgg aaaggagttc aataaaaaaa gaatccttta ctacgatgat
     1081 ttcccgtccg aacaggcctt gatagaaagg ataatggaaa ttgagaatga cccgacgcag
     1141 gccctgaaaa tcttgaaaga gaaggcattt gccggtgaaa aattcaatat ctccaggacc
     1201 ggcaacgatc tgctgaactt tctggttgcc tctatcgaaa gcatggctgg gagaaagcct
     1261 gtcgcactac ggcccttgaa atcgatgagg cattctttca aaatgcaccg cagcagagtg
     1321 atcaatagac tcgcactcct gagaagactg gagatgacgg ctcaaaaact aaaactcagc
     1381 tagaatgaag gtggtaatct tcgccggcgg tatgggtacg cgaatctccg aagaatccca
     1441 tttgaagccc aaaccgatga tcgagattgg ggacaagccc atcctgtggc atatcatgaa
     1501 aatattttct tcccagggat tcaacgattt tattatctgc ctggggtata aagggtatgt
     1561 gatcaaggaa tattttctaa attatttcct gcataattcg gacgtgacga ttgaattgaa
     1621 agataacaag gtcagcgtcc ataacacgaa agcggaggat atcaaagtta ccctggtgga
     1681 gaccggctta caaactcgca cggccggcag gcttaggaga gtaagacagc acttgcaggg
     1741 agaggatttt atgctcactt atggagacgg attggcggac atcgacctgc gcagcctggt
     1801 ggctttccat aaatcgcacg gaaaaattgc cagcgtgacc gccattcagc cactgggcag
     1861 attcggagtc atgcaaataa acagcgataa ctcggtggat gtttttaagg agaaagtgaa
     1921 agaggacgag ggctggatca acggcggatt cttcgtcctg aaacctgcgg tcttcgacta
     1981 tctgccagtg aacgccgatg agcttatgtg ggaagacctg ccgatacaaa accttgcaaa
     2041 ggacgatcag ctgatggctt atcaacatca tggcttttgg aagtgcatgg acgcattgag
     2101 agataaaata gaattggagg agttgtggaa ctcggccaat gccaaatgga agatctggca
     2161 atgaagacgt tattcggagg cgtttacagc gacagaaagg tgctggttac gggccatagc
     2221 ggattcaagg gctcatggtt attactttgg ttgaagcatt tgggctcaaa cgtaacaggc
     2281 ttttccctgg cgccgtcaac ggctccctcc catattgagc tgctcgggtt cttcgatcct
     2341 tcatctgccg gagatatcag gaatatggat cagctgataa aggctttcac ggattttcag
     2401 cctgagatcg tctttcatct ggctgcacag ccgctggtaa gaaggtcata tgccgacccg
     2461 ttggaaacat tcacaaccaa tataaccgga acggcaaacg tgttggaggc ggccaggcat
     2521 accccctcgg taaaggcgtt tgtaaacgtt acaaccgata aagtttaccg gaataatgat
     2581 tggccccgcc cataccggga gacagatatg atcgggggtc acgacccgta cagtacatcg
     2641 aaagcctgcg tggaactatt acatgaatct ta
//
LOCUS       DATGKE010000349         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1400525, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000349 DATGKE010000000
VERSION     DATGKE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400525"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VKU83_06000"
     CDS             complement(<1..318)
                     /locus_tag="VKU83_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HLI93138.1"
                     /translation="MKPTATISQNHSSLLHPIARSAILSASFSTPERTGTDPAGFVSA
                     LVHEIRNPLCTINLAIEMLSMMELDEEQKYYLSMIVRGSGRINELVSHLLTLDLAKET
                     GNEV"
     gene            348..983
                     /locus_tag="VKU83_06005"
     CDS             348..983
                     /locus_tag="VKU83_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93139.1"
                     /translation="MNIPLLAAVTCFLKSVTSFTHHNEDANHVVFRLNRIAIKKVKRR
                     NFTINYKKGIMKKALYLIPAGLLFFCASVTAQKANIDSLALVAQIGRDQLELGKLQNM
                     VSQKTKNKEGAAVNAQNSASDNANAAERLNGDPDNKKLASNASNKAGKAKSDARKSRK
                     QSGQLDDLNQNILDLKSKIASEQYKLSIYSLTPPVITPTLKPVVIDTTLHP"
     gene            1074..>2060
                     /locus_tag="VKU83_06010"
     CDS             1074..>2060
                     /locus_tag="VKU83_06010"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLI93140.1"
                     /translation="MKRINFHPGNMLQNPLSAHPVGVNAGKGNLMDYVLRVPSYGWMD
                     AHGGLIVPNARQVLHEFWSRLNIFKTKKNWLAFFNWMEVLLLIPCLFLFFFRYFSFPL
                     LGIAFVYGMIVMGTHGTIWYHRYCTHSAYTFSNQFWRLVTRNLTINIVPEETYVISHH
                     VHHAKSDQPGDPYNASAGFLYCFLADVNHQAISENLTEEEYIRVVKMMAGSGIKPNTY
                     RQYRKWGSVVRPAWHLGGWILNWSFWGTVFYLAGGAALVFALFGAAGFWAIGIRTFNY
                     EGHGKGKVKHREGIDFNREDLSINQLWPGYVAGEWHNNHHLFPSSARSGFLNY"
BASE COUNT          554 a          465 c          508 g          533 t
ORIGIN      
        1 cacttcattg ccggtctctt tggccagatc aagcgttaac aggtggctta ccagctcatt
       61 gatccgtccc gatcccctca caatcatgct gaggtaatat ttttgttctt cgtcaagctc
      121 catcatgctc agcatctcga tggccaggtt tatcgtgcat aacggatttc tgatctcatg
      181 gacaagagca gaaacaaaac cggcaggatc agttcctgtt cgttcagggg tcgaaaaaga
      241 cgctgagagg atggcggatc gcgcaatagg atgaagtaat gaactgtgat tttggctgat
      301 agtggcagtt ggtttcattt ctccgataat tttagcttga cgtaaaggtg aatattcctt
      361 tgcttgcggc cgttacatgc tttcttaaaa gcgttacatc tttcacacac cacaatgaag
      421 atgcgaatca tgtagtattt cgcttaaatc gtatagcgat caagaaggta aaaaggcgga
      481 attttaccat taattataaa aaaggtatta tgaaaaaagc actttattta attccagccg
      541 gcctgctgtt tttctgcgct tcggttacag cacagaaagc gaatattgac tcacttgcat
      601 tggtcgcaca gatcggccgg gatcagcttg aacttggtaa actacaaaac atggttagcc
      661 agaaaaccaa gaacaaggaa ggagccgcgg tcaatgcgca gaattcagcc agcgacaatg
      721 ccaatgcggc ggaaaggtta aacggcgatc cggataataa gaaactggcc agcaatgcca
      781 gcaataaggc gggaaaggcg aaaagcgatg ccaggaaatc gaggaagcaa tccggacaat
      841 tggatgacct gaatcagaat atcctggatt tgaaaagcaa aatagccagc gaacaataca
      901 aattgagtat atattctctg acgccacccg tcataactcc cacgctgaag cccgtcgtaa
      961 ttgatacgac cttgcatccc tgaatggtct tactcgctgg cgataaaatg cgtgaattat
     1021 ataacattca tccaataata atgtaatatc caaccgatgg ggatgcatta aatttgaaga
     1081 ggatcaattt ccaccccggc aatatgcttc aaaatcctct atctgcgcat cctgttggtg
     1141 tcaatgcagg gaaaggaaac ctgatggact acgtattgcg ggttccttcc tatgggtgga
     1201 tggatgctca cgggggtctg atcgtcccca acgcccgtca ggtgctgcat gagttctggt
     1261 cccggctgaa cattttcaag acaaaaaaga attggctggc ctttttcaac tggatggagg
     1321 tgctactgct gatcccctgt ctgttcctct ttttttttcg ttatttctcc tttcccttgt
     1381 tagggattgc ctttgtctac ggcatgatcg tgatgggtac gcatggcaca atatggtatc
     1441 accggtactg cacgcatagc gcatatacgt tcagtaacca gttctggcga ttagtaaccc
     1501 gaaatctgac gatcaacatt gtccctgaag aaacgtatgt gatctcgcat cacgttcatc
     1561 atgccaagtc ggaccagccc ggcgatccat acaatgccag cgccggtttt ttgtattgtt
     1621 tcctggcaga tgtcaaccac caggcgatct cggagaattt gacagaagag gaatatatcc
     1681 gtgtcgtcaa aatgatggcg ggcagcggca tcaagccgaa tacgtaccgg caatatcgga
     1741 aatggggatc tgttgtcagg ccggcctggc atttgggtgg ctggatcctc aactggagct
     1801 tctgggggac tgtcttttac ctggcggggg gtgcggcgct tgtattcgca ttattcgggg
     1861 ctgcgggctt ctgggccatc gggatccgaa cgttcaatta tgaaggacat ggaaagggaa
     1921 aagtcaaaca cagggaaggt atcgacttta acagggaaga tctatcgatc aaccagctat
     1981 ggccgggata cgttgccggc gagtggcata acaatcatca tctctttccg tcgagcgcac
     2041 gcagcggctt tttaaattat
//
LOCUS       DATGKE010000350         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1458040, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000350 DATGKE010000000
VERSION     DATGKE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458040"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..907)
                     /gene="rsmA"
                     /locus_tag="VKU83_06015"
     CDS             complement(128..907)
                     /gene="rsmA"
                     /locus_tag="VKU83_06015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLI93141.1"
                     /translation="MMPMYTLKKSLGQHFLKDENISKKIVAALRERPFDRLLEVGPGG
                     GALTKYLLALEGVDFRAVELDEEKVRHLLSRWPGLQGKIIPGSILDLPKPFQERFTVI
                     GNFPYNISSQILFKMLDWKEDLQTVIGMFQKEVALRVAAKEGSKVYGVLSVLIQAFFK
                     VEYLFEVSEASFTPPPKVKSAVIRLTPAPPPGFRDEASFFLLVRTAFNQRRKTLRNAV
                     RSLFDEATLRDPVFDRRAEQLSITEFAGLTIKMKNNQFLIK"
     gene            1005..2099
                     /gene="pdxA"
                     /locus_tag="VKU83_06020"
     CDS             1005..2099
                     /gene="pdxA"
                     /locus_tag="VKU83_06020"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLI93142.1"
                     /translation="MSNPSQRPVIGISIGDLNGIGAEIIIKTFSDHRLLELCTPLIFA
                     SNKLINFYRKSTPEINFNYQSTRDYSRITPKQINIYNCWEEEVAIEPGKLTDIGGSYA
                     VKSLVTAAEALRDKKIHGLVTAPIHKKNTQTANFDFTGHTPYLKHVFNAADVLMLMVS
                     DTLKVGLVTEHVPVKDVARYITREAIQSKLRILSESLMRDFGIDKPKIAVLGLNPHAG
                     DEGLIGKEEEEIIKPAIKDAKHQMLVYGPYSADAFFARNHQSRFDGILAMYHDQGLIP
                     FKSLSGGDGVNFTAGLSMVRTSPDHGTAFDIAGKNKADASSFLAAIYTCIDIIRNRQE
                     FDDNRSNPLKKMGAIVLANAEDEKIEGSRG"
     gene            2362..>3326
                     /locus_tag="VKU83_06025"
     CDS             2362..>3326
                     /locus_tag="VKU83_06025"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLI93143.1"
                     /translation="MKRNFLTIVLFGCCSFLSTRAAGIADNFNSRPGVRIAGVKGYLQ
                     GRCWFFSAFDVNRNGWAPNIEGDGAMVSGTGASPTERTGIYTPVLDMPVSTRVSFSYC
                     FNQSVGDRRWLKVFAADADDKPLFLLDSLELTGSEAGHIYTYDKPLTLPAAGLYKLIL
                     NYQGIGGTERIAIDQFAVSAPLHYASTCNTAPVALKDKFAGTASHTATGNVLSNDYDP
                     DHETMTAYLLSESPDGRVDLNKDGEFVFTPKEGFTGNSTQFSYKVCDNGSPALCSMTT
                     TATIRFPARSSLTGFQALYSHKAVAISWATGAGNHSTTFEVERSL"
BASE COUNT          758 a          962 c          870 g          736 t
ORIGIN      
        1 tcggcatcgc cgatggacgc cagcagttcc tcgtagctga tctgtggggt ataccggacc
       61 gaatagccct tgctggccag cgcctgctca agataatcat gggctttggc ggtgatgatt
      121 actgtcttca tttgatcaaa aactggttat tcttcatttt tatggtcagc ccggcgaatt
      181 cggtaatgct gagctgttcg gcccgccggt cgaacaccgg atcccggagc gtggcctcat
      241 cgaaaaggga ccgaaccgca ttgcgaaggg tctttctgcg ctggttaaac gccgttcgca
      301 ccagcaggaa gaagctggcc tcatcgcgga atcctggtgg cggggccggc gtaagccgga
      361 tcacagcgct tttcaccttc gggggcgggg taaagctcgc ctcgctgacc tcaaacaaat
      421 attccacctt gaagaaagcc tggatgagca cgctcagcac gccatacact ttcgatcctt
      481 cctttgccgc gacccgtagc gccacttctt tctggaacat gccgatgacg gtctgcagat
      541 cctccttcca atcgagcatc ttaaacagga tctgcgacga aatgttgtac ggaaaattcc
      601 cgatgaccgt gaatcgttcc tggaatggct tcggcaggtc gaggatactt cccgggataa
      661 ttttgccctg caggcccggc catcgggaca agaggtggcg gaccttctct tcatccagtt
      721 ccaccgcccg aaagtcgacg ccttccagcg ccagcaggta tttggtcagt gcgccgcccc
      781 cagggcctac ttccaaaagg cggtcgaaag gacgttcgcg cagggccgca acgatctttt
      841 tggaaatatt ctcatccttg aggaagtgct ggccgagcga ttttttcagc gtatacatag
      901 gcatcatgtc ggcaattttg accctcccgg tcaaatatgc cagccggaca gcctccggcg
      961 aagtgcaaaa gtcgtatttt tgaccggtaa ataagcatca gcgcatgagc aatccatccc
     1021 aacggcctgt gataggaatt tccatcggcg atctcaacgg gatcggtgcc gagatcatca
     1081 tcaagacctt ttccgatcac cggctactcg aattatgcac gccgttgata ttcgcgtcca
     1141 ataagctgat caatttctac cgcaagtcga cgcccgagat caatttcaat taccagagca
     1201 cccgggatta ttcgcggatt acgccgaagc agatcaatat ctataactgt tgggaagagg
     1261 aggtcgcgat cgagcccggc aagctcactg acatcggcgg atcctatgcc gtcaaatccc
     1321 ttgtgacagc cgcagaggcg ctccgggata aaaagatcca tggcctcgtc acggcgccga
     1381 tccacaagaa gaacacgcaa accgccaact tcgacttcac cgggcataca ccctacctta
     1441 aacatgtttt taatgccgcc gatgtcctca tgctcatggt atcggatacc ctgaaggttg
     1501 gcctggtgac cgagcacgtt cctgtaaagg atgttgcccg atacatcacg agggaggcca
     1561 ttcagtccaa attgcgaatc ctcagcgaga gcctgatgcg cgacttcggg atcgacaagc
     1621 cgaagatcgc cgtcctgggg ctgaacccgc atgcgggcga tgaaggcctt ataggaaaag
     1681 aagaggaaga gatcattaag ccggccatca aggatgcgaa acatcaaatg ctggtttacg
     1741 gcccctacag cgccgacgct ttttttgccc gcaatcacca gtcccgtttc gatggcatcc
     1801 tcgcgatgta ccacgaccag ggcctgattc ccttcaagtc gctctccggc ggcgatggag
     1861 tgaatttcac cgccggactg tccatggtgc gcacctcgcc cgaccacggc acggccttcg
     1921 atatcgcagg gaagaacaag gcggatgcct cctcctttct tgcggcgatc tatacctgca
     1981 ttgacattat ccgcaaccgg caggaattcg atgacaaccg cagcaatccc ttgaaaaaga
     2041 tgggcgcgat cgtgctggca aatgcggagg atgaaaagat cgagggcagc aggggctgag
     2101 gaacctgctg attatcaacg tcagggcccc ggcggccaat tttattggcc ggacgcttgc
     2161 aaaaccaaaa tctgttttct acctttggtg cattcccggc gtccatttgc cggaccttgg
     2221 gcccgccccc agccgtagcg ctaaatttat tatcccccga aaaacaggta ttcagtctcg
     2281 atcatcgttt gttcccctcc tttgaatcac cgctgatttt cagcatccta ttgctttagt
     2341 caccttctga aaattacaaa gatgaaacgg aacttcctga cgatcgtcct ttttggctgc
     2401 tgttctttcc tttcaacccg ggcggccggg attgctgaca atttcaattc ccggcccggc
     2461 gtaaggatcg ccggggtaaa gggctaccta caaggccgct gctggttctt ttccgctttt
     2521 gatgtcaatc gcaatggttg ggcgccgaat atcgaaggtg atggcgcgat ggtatccgga
     2581 acgggcgcca gcccgacgga gcgaaccggt atatatactc cggtgctgga tatgcctgtg
     2641 agcacgcggg tttctttcag ctattgtttt aaccagtctg tcggcgaccg acgctggctt
     2701 aaggtctttg ccgcagacgc cgacgacaag ccgctgtttc tcctcgacag cctcgagctg
     2761 accgggtccg aagccggcca tatctatacg tacgacaaac cgctgacgct tccggctgcg
     2821 ggattatata agctgatcct caattaccag ggtattggcg gaaccgagcg catcgccatc
     2881 gatcagttcg ccgtcagcgc accgctgcac tacgcgtcca cctgcaacac ggcgccggtt
     2941 gcgctcaaag ataaattcgc cggaacagcc agccacacgg ccaccggaaa tgtgctcagc
     3001 aatgattatg atccggatca cgaaacgatg accgcctacc tgctgtcgga atcgccggac
     3061 ggaagggtag acctgaacaa ggacggtgaa ttcgtgttta cgccaaagga aggctttacc
     3121 ggcaactcta cacaattttc ctataaagta tgcgataacg gaagcccggc gctttgctcc
     3181 atgaccacca cggcgactat ccgttttccc gcccgtagca gcctgaccgg ctttcaggca
     3241 ctttatagcc acaaggccgt ggcgatcagc tgggccaccg gcgcaggcaa ccatagcacc
     3301 acgtttgagg tcgagcgaag ccttaa
//
LOCUS       DATGKE010000351         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_400571, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000351 DATGKE010000000
VERSION     DATGKE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400571"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VKU83_06030"
     CDS             <1..300
                     /locus_tag="VKU83_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93144.1"
                     /translation="PNFWQKKRLAQHNHTIWVFYIFSILAILFAVIGIRYGNDPGRIK
                     ALPIIACVLVVICGIIAVASIDTSHEKEFQLPKIQYDSLKNAGAIDHYIDQHLLQ"
     gene            297..689
                     /locus_tag="VKU83_06035"
     CDS             297..689
                     /locus_tag="VKU83_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93145.1"
                     /translation="MITYELSLIFPVGFLLALWHKHLIDKGRHIQHGWWALLFGFLVV
                     GVIGWKRTIFLPIGWITGVFFTACMIGHQVFFNIVLNRLRKLPWDYLNPKGKSIIDGI
                     EYRLFGKKTWLVEVMLAVIWIILQLWII"
     gene            702..1262
                     /locus_tag="VKU83_06040"
     CDS             702..1262
                     /locus_tag="VKU83_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HLI93146.1"
                     /translation="MADFKLFWPLLSENEGYYANDPADSGGETWRGISRNNYPGWSGW
                     AIIDSYKKGSAFAGAHEANTLLKPDATLEAKVIAFYKSSQWDVIQADAMQNQSIANFL
                     ADWGVNAGMSVPIKHVQRILGLDVDGKVGPHTLAALNSASGPDLFKKLQADREQFYMD
                     VVTVHPDNRKFLTTWLKRNASFSYQA"
     gene            1298..1558
                     /locus_tag="VKU83_06045"
     CDS             1298..1558
                     /locus_tag="VKU83_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93147.1"
                     /translation="MSVVVRVKVNSKQISNHWTKDEATGKPKVNTTVNMNTVYTNDPN
                     NPNFIFSVMSGGTKFELQTINDNAADEFELDGEYECVFTRVK"
     gene            1693..2109
                     /locus_tag="VKU83_06050"
     CDS             1693..2109
                     /locus_tag="VKU83_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93148.1"
                     /translation="MGLENPKRCRPKGKLSTMNPFNREEHSHRLDRELLYMTLENQFL
                     IFQKLEFMADELKNLQDAAAKTLTVEEAVLALLTSQKATIDSLSKQLVDAIAAGNPAA
                     LQAVADSLNTESDKVNAAIAALQPATPAAAPETPAQ"
     gene            2277..2687
                     /locus_tag="VKU83_06055"
     CDS             2277..2687
                     /locus_tag="VKU83_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93149.1"
                     /translation="MENKFESEIGLRDEVRLNIGQGQTVNAYVTAIKFYKDGDVTYDL
                     NVQKDGGIVTVQDVHFACLTKGWAKQEHSLLPALGEICRQDTCNRRESPMGKVHWMIH
                     DACYELSIGNLDKAKAILADADKFTHDAIDKETA"
     gene            2789..3034
                     /locus_tag="VKU83_06060"
     CDS             2789..3034
                     /locus_tag="VKU83_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93150.1"
                     /translation="MNTIIRDEERQRLHRDYLREVDPFIRELSNILHLAPCSYFINAT
                     TGEIEKLPYPAEIQKLVDEINGFIESITSKYQKLIES"
     gene            3391..>3806
                     /locus_tag="VKU83_06065"
     CDS             3391..>3806
                     /locus_tag="VKU83_06065"
                     /inference="COORDINATES: protein motif:HMM:NF036423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HLI93151.1"
                     /translation="MHAYTIRPVCWTHKVNKSGTAPVMIAVTIARKVRYFKTPYRIEP
                     TQWENNQVVRHANAVLINRAIRSQIHELERDILLREAEGETVTGRNVKQTKLVNVFDF
                     VLDVSKNLPPATRRRYTCEVGRLKAFAGDYLEWREIT"
BASE COUNT         1061 a          936 c          934 g          875 t
ORIGIN      
        1 cccaatttct ggcaaaagaa aagactggcg cagcacaatc ataccatatg ggtattctat
       61 attttctcca tccttgctat actctttgcc gttattggca ttaggtatgg gaacgaccca
      121 gggagaataa aggcactgcc aattatagcg tgtgtactgg tggtgatctg cggaattatc
      181 gccgtcgcct ctatcgacac ttcccacgaa aaagaattcc agttgcctaa gattcagtac
      241 gatagtctga aaaacgctgg cgctatcgat cattacattg accaacacct gttacaatga
      301 tcacctacga actctcccta atcttccccg tcgggttcct gctcgcccta tggcacaagc
      361 acctgatcga taagggtagg cacatccagc acggctggtg ggcgctcctt ttcgggttct
      421 tggttgtcgg ggtcatagga tggaagcgaa ccatattcct gcccattggc tggatcacgg
      481 gggtattctt caccgcctgt atgatcggcc atcaggtgtt cttcaatatc gtcctgaatc
      541 gactgcgaaa gctgccctgg gactatttga acccgaaggg gaagtcgatc atcgatggca
      601 ttgaataccg gttattcggc aaaaagacat ggctggtgga agtaatgctg gccgttattt
      661 ggatcatttt acaattgtgg attatttaaa atcggtatat catggctgac ttcaagctat
      721 tctggcccct gctctctgaa aacgagggct attacgcgaa cgatccggcg gactccggag
      781 gcgagacatg gcgcggcatc tcccggaaca attacccggg ctggtccggc tgggccatca
      841 tcgacagcta taaaaagggc tccgcgtttg ccggagccca tgaagccaat acgttactca
      901 aacccgacgc cacactggaa gccaaggtca tcgcctttta caagtcttcg caatgggatg
      961 tgatccaggc agacgccatg caaaatcaga gcattgcgaa cttcctggcg gattggggag
     1021 tgaatgccgg catgtccgta ccgataaagc acgtgcagcg gatcttaggg ctggatgtag
     1081 atggcaaagt aggcccgcat acattagcgg cgctgaacag cgccagcggc cccgacctat
     1141 tcaaaaagct ccaggcagac cgggaacagt tctatatgga tgtagtaacc gtccatccgg
     1201 acaacaggaa gtttctaact acctggctca aaaggaatgc atcattttct tatcaagcat
     1261 aaacccgtgg acaggggata aactgagcat caattttatg tcggtagtag taagggtaaa
     1321 ggtaaactca aagcaaattt ccaatcattg gacaaaagac gaggcaacgg gaaaacccaa
     1381 agtcaatacg accgtcaata tgaacacggt atatacgaac gatccgaata acccgaattt
     1441 tatcttctct gtgatgagtg gagggaccaa atttgagctc cagaccatca atgataatgc
     1501 tgcggatgag ttcgagcttg atggagaata tgagtgcgtg ttcacgagag tcaaataacc
     1561 tatggcccgg cgccgcccaa agtaccccga aaagccctgg catcccgacc gtgcccgctg
     1621 gaagggattc atggtgaagg gggatgtagc taaacgcgac gtccccaatg agcccaaagg
     1681 cggctggcag ctatgggact ggaaaaccct aaaaggtgta gaccaaaagg aaaactatca
     1741 acgatgaacc cattcaatag ggaagagcat tcgcaccggc tcgaccggga actgctctat
     1801 atgactttag aaaaccaatt tttaatcttt caaaaacttg aatttatggc agacgaattg
     1861 aaaaacttac aggatgcggc agccaagaca cttaccgtgg aagaggctgt cctcgcattg
     1921 ttgaccagcc aaaaggcgac tattgacagt cttagcaagc aattggtgga cgcgatcgcg
     1981 gcaggcaacc ccgccgcatt acaggcggtg gccgatagcc tgaatacgga atctgacaag
     2041 gtaaatgccg ctattgcagc attacagcct gctacgcctg ccgctgctcc cgaaacgccc
     2101 gcgcaataaa gaagctcgaa atcggagcaa ttttttcata gcaacggccc cggtttctac
     2161 tggggcctct ttttcataga gcatagtttt tggtgaaccc tggcgaaagt cagggttttt
     2221 tgtcgtttta cgggttcccg taaaaaagta tccatgggat atatttcctt tgtcttatgg
     2281 aaaacaaatt tgaaagcgag attgggctcc gggatgaagt ccgtctcaat ataggccaag
     2341 gccagaccgt taatgcatat gtaacagcca tcaaatttta taaggatgga gatgtcacct
     2401 acgatctaaa tgtccaaaaa gatggcggta tcgtcaccgt gcaggatgtc cactttgcat
     2461 gcctgacaaa aggatgggca aaacaggagc atagccttct gccggccctc ggtgaaatat
     2521 gtcggcagga tacatgcaac cgccgagaaa gcccgatggg aaaagtccac tggatgattc
     2581 atgatgcttg ttatgaatta tccataggta acctagacaa agccaaagcg atacttgctg
     2641 atgccgacaa attcacccac gatgcaatcg acaaagaaac tgcatagata gcgcaatgca
     2701 gcgccgaagg gcggcctcgg gagtagctac tcggggtttt ctttgaaacc ctggccgtaa
     2761 atggctgggg tttttgtatc ttaggggtat gaacacaata atacgtgatg aggaaaggca
     2821 aagacttcat cgtgattatc tgcgagaagt cgatcccttt ataagggaat tgagcaatat
     2881 attgcacctt gcaccgtgca gctatttcat aaatgccaca accggagaga tagaaaaatt
     2941 gccatacccg gcggaaatac aaaagcttgt cgatgaaata aatggcttta ttgaaagcat
     3001 tacttccaaa tatcaaaaac tgattgaatc gtgaacgcat gtccccgaca tccatgtcgg
     3061 agacatctcc taccttcgcc ccatgccccg ctcccgcaca cccgctcatg atccccgcct
     3121 ggatatcata cctgccctgc cggagctgca ttagatgggg gaggggaggg caaaaacact
     3181 gactttgttc tacccataaa aactaacggc gttagactaa ttgattatca gtatatttaa
     3241 aaatacggtt gaatccaatc cgggtcactt taagcccgcg tccagcgcgg gttttcttat
     3301 ttcacgcggg tttcacaatg caatgatata cctaaaagtg caaaaatgta tatattcgtt
     3361 ttacctattg tttaacccac tgttttacca atgcacgcct ataccattcg cccggtctgc
     3421 tggacacaca aggtcaataa atcagggacg gcccctgtca tgatcgccgt taccattgcc
     3481 cgaaaggtac ggtatttcaa gacgccctac aggattgagc ccacccaatg ggaaaataac
     3541 caggtggttc gccatgccaa tgccgtgttg atcaaccggg ccatccggtc ccaaatccac
     3601 gagctggagc gagacatatt attgcgcgag gccgagggcg aaacagtcac cgggcggaat
     3661 gtcaagcaga ccaaactggt gaacgtcttc gatttcgtcc tggacgtatc caagaatcta
     3721 cccccggcca cccgccgccg gtatacctgc gaggtaggca ggctaaaagc cttcgccgga
     3781 gactacctgg aatggaggga gatcac
//
LOCUS       DATGKE010000352         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_683072, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000352 DATGKE010000000
VERSION     DATGKE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683072"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /gene="lepA"
                     /locus_tag="VKU83_06070"
     CDS             <1..1134
                     /gene="lepA"
                     /locus_tag="VKU83_06070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HLI93152.1"
                     /translation="DRVKFVSTNLEYEADEVGVLKLTLDPRAEVGTGDVGYIITGIKN
                     AKEVKVGDTITLASNPKPAMIQGFQEVKPMVFAGIFPVNTDDFEELRDCMDKLQLNDA
                     SLTYELETSQALGFGFRCGFLGMLHMEIIQERLEREFNQTVITTVPNVSFVAYTNKNE
                     KIVVNNPAEMPDPTRIDHIEEPYIRAQIITKPEYIGNIMTLCLGKRGILINQHYLTTT
                     RVELIFELPLTEIVFDFYDKLKSQTRGYASFDYHPIDRRESDIVKMDILLNGEKVDAL
                     SALIHRSRSQDFGRKLCEKLKELLPRQQFQIAIQAAIGAKVIARETISALRKDVTAKC
                     YGGDISRKRKLLEKQKEGKKRMRQIGNVEVPQEAFLAVLKLDE"
     gene            1138..1779
                     /locus_tag="VKU83_06075"
     CDS             1138..1779
                     /locus_tag="VKU83_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLI93153.1"
                     /translation="MRTPLQKNEKILLVTYSSWTVLLFPALLSLALVVAGVIVVVKLP
                     SVYGWLIALAGIIYWLLRYSAWKVNIWVVTNFRVIDEAGLLNHYAKESPLDKINNVSY
                     DQSLAGRLFNFGHVEIQTAAEAGATDYYDVNHPKRLKDTITAAQAEYKNWQYGNQAAQ
                     MAAAMDARYSPQPKAPDAPAIAQELEKLFELKMKGALSEDEYNRAKARLLNHG"
     gene            complement(1770..>2736)
                     /locus_tag="VKU83_06080"
     CDS             complement(1770..>2736)
                     /locus_tag="VKU83_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93154.1"
                     /translation="VCVIAFGSGLSAQSRISPENLAALQQKEDGMKQFAYDIVNGETA
                     ATRFRSDSSFIRSLVKALKLPYSFYYPFDSLQTISRLYAPDSSFRIFTWEFKKDNMMY
                     LQEGAIQMNQPDGSLKLFPLFDASMFTGNPLDSVRTRLNWIGAIYYRIIEKQWQGRNY
                     YTLLGFDDYSETANKKWMEVLTFTPEGEPRFGGPYFSFANDSVPKPTQYRFDIEYKKE
                     AGTRFNYDPSLDLIVFDELISESNQPQNKDTYVPDGDFEAFQWKDGKWVHLQKGIVTV
                     KLKDGDFPREAEILNDQGKANEDVLQAQSEKNRKKAPARPSPPHP"
BASE COUNT          661 a          704 c          778 g          593 t
ORIGIN      
        1 gacagggtca aattcgtgtc gaccaacctt gaatatgagg cggacgaggt tggcgtcctc
       61 aagctgacgc tggacccacg ggcggaagtc gggaccggtg acgtcggcta catcatcacc
      121 gggataaaga acgccaagga agtaaaggtc ggcgatacca ttacgctcgc atcgaatccc
      181 aagcctgcca tgatccaggg tttccaggaa gtcaagccga tggttttcgc ggggattttc
      241 cccgtcaaca cggatgattt cgaagaactt cgcgactgta tggacaagct gcaattgaac
      301 gatgcgtcgc tgacctatga gctggagact tcacaggcgc tcggcttcgg cttccgttgc
      361 ggcttcctcg ggatgcttca tatggagatc atccaggagc ggctcgagcg cgagttcaac
      421 caaacggtta ttaccacggt accgaacgtg agtttcgttg cctacacgaa caagaacgaa
      481 aagatcgtgg tcaacaatcc cgctgaaatg cccgacccga cgcgcatcga tcatatcgaa
      541 gagccttata tccgcgctca gatcatcacc aagccggaat acatcggcaa tatcatgacc
      601 ctctgcctgg gcaagcgggg tatcctcatc aaccagcatt acctgacgac gacgcgcgtg
      661 gagctgatat tcgaactgcc cctcactgag atcgtctttg acttctacga caagctgaaa
      721 agccagacac gcggctatgc ttcttttgac taccatccca tcgaccggcg cgagagcgat
      781 atcgtgaaaa tggatatcct tttgaacggc gagaaggtgg atgcgctcag cgcgttgatc
      841 catcgaagcc ggtcccagga ttttggccgg aagctgtgcg aaaagctgaa agagctcctg
      901 ccgcgccagc aattccagat cgctatccag gccgcgatcg gcgcaaaagt gatcgcgcgg
      961 gagacgatca gcgccctgcg gaaagacgtg acggcaaaat gttatggggg cgacatcagc
     1021 cggaagcgca agctgctgga gaagcagaag gagggtaaga aacgaatgcg gcagatagga
     1081 aatgtggagg tgcctcagga agcattcctg gcagtgctca aactcgatga atagtctatg
     1141 cgaactcctc ttcagaaaaa tgaaaagatc ctgctggtga cgtacagcag ttggacggta
     1201 ttgctttttc ccgcgctgtt gtcgcttgcg ctggtcgtag ccggcgtgat cgttgtcgtg
     1261 aagcttccgt ccgtatacgg atggctgatc gcgctggcgg gtatcatcta ctggctgctg
     1321 cgctattcag cctggaaagt aaatatctgg gtcgtgacca atttcagggt catcgacgag
     1381 gccgggttgc tgaaccacta tgcaaaggaa agcccgctgg acaagatcaa caatgtatcc
     1441 tatgaccagt cactggccgg cagattgttc aatttcggcc atgtcgagat ccagacggca
     1501 gccgaggccg gcgctacgga ttactacgat gtgaaccatc ccaagcggct aaaggatacc
     1561 atcactgctg cccaggcaga atacaagaac tggcagtatg ggaaccaggc ggcccagatg
     1621 gccgcggcga tggatgcccg ctacagcccg cagccgaagg ctccggacgc accggcgatc
     1681 gcccaggaac tcgaaaagct gtttgaatta aagatgaaag gcgccctgtc cgaggatgag
     1741 tataaccggg cgaaggcgcg gttgcttaat cacggatgag ggggagacgg acgggcagga
     1801 gctttcttcc ggttcttttc cgactgggcc tgaagcacgt cctcgtttgc tttgccctgg
     1861 tcgttcagga tctccgcttc gcgcgggaaa tcgccatcct tcaatttgac agtaacgatg
     1921 cctttttgca aatggaccca ttttccgtcc ttccactgaa aggcttcaaa atcgccgtcc
     1981 gggacataag tatccttgtt ttgcggctga ttactctctg aaatcaattc atcgaacaca
     2041 atgaggtcca gcgaggggtc atagttgaac cgggtgccgg cttctttctt gtattcgata
     2101 tcaaaacggt attgggtagg tttcgggacc gagtcgtttg caaaggagaa gtacgggccg
     2161 ccgaaccgcg gctcgccttc gggagtaaag gtgagcacct ccatccattt cttgttcgcc
     2221 gtctcgctgt agtcgtcaaa accgaggagc gtatagtaat tccttccctg ccactgcttt
     2281 tcgatgatgc gataatagat ggcgccgatc caattgagcc gcgtacgaac cgaatccagc
     2341 ggatttccgg tgaacattga agcatcgaag agggggaaga gctttagtga cccatcaggc
     2401 tggttcatct ggatggcgcc ttcctgcagg tacatcatat tatccttttt gaattcccag
     2461 gtgaagatgc ggaaactgct atccggcgca tatagccggg aaatggtttg aagggaatcg
     2521 aaaggataat agaaagaata gggcagtttc agcgctttga cgagtgaccg gataaagctg
     2581 ctgtcggaac gaaaacgggt agccgccgtt tcaccgttga cgatatcata ggcgaactgt
     2641 ttcatcccat cctccttttg ctgcaatgcc gccaggtttt ccggggagat acgggattgg
     2701 gcggacaggc cggatccgaa ggcgatgacg cagacg
//
LOCUS       DATGKE010000353         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_221737, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000353 DATGKE010000000
VERSION     DATGKE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221737"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="VKU83_06085"
     CDS             <1..1947
                     /locus_tag="VKU83_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93155.1"
                     /translation="VRIDNGFDLKVGGVYEDLPRNTRFYETACLLPWENQANWLASKD
                     PQSNWYNSCGFLYVQLKDNVTMDEAATSVRNAPTEHIREYEEHATLYPLDKLHLYTDF
                     TNGTMSGGRIQTIRMFGIIGAFVLLLACINFMNLSTARSERRAKEVGIRKAIGSLRRQ
                     LVAQFITESLAMAFLSMGLALLLTQLALPFFNDLADKQMALPWASPAFWSWLFAFTLL
                     TGLIAGSYPAFYLSGFRPIKVLKGTARFRPRTSVSKQLGRWSLRVGRWAALPRRALVV
                     VQFTISMAMIIGTIIVFRQIRYAKDRAVGYTREGLIAVNMNTGDLYAHSEAIRATLLQ
                     TGAVTDMGESNSLPTEISSGNDGFYWKGVEPGNSYGFGTIGVDFDFGHTIGWQVTEGR
                     DFSRHFPADTARNRGGLIVNEAAVKQMGLKHPVGEIVHYKGTIFPNVDHVIVGVVKDM
                     VMESPYEKPRPVFFLCGGWVNYMVIRIDPAMPVRDALAKIEPVFKQYNPGSPFEFHFI
                     DDTYAKKFADEERIGRLAGVFAALAIFISCLGLFGLASFMAEQRVKEISVRKVLGATI
                     FNLWQLLSKDFIVLVLLACCFSIPLAWYLLHGWLQHYDYRTNISAWIFVAVGAGALAI
                     TLLTVSWQAIRAALANPAKHLRTE"
     gene            complement(1959..>2801)
                     /locus_tag="VKU83_06090"
     CDS             complement(1959..>2801)
                     /locus_tag="VKU83_06090"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HLI93156.1"
                     /translation="MENEAAWRPEAETRAGVGRIWNVMRDCVYRGCHAEGQLPGGLHV
                     QRRAAALSRRLIGDSSYHDCDSWVQAIRSGGNGFKYTLDWVSCFALAVNEENASFGRV
                     VTAPTNGAAGVIPAVLHYFVIFCDSYNEEKIYRFILTASEIGSIFKKGATISAAMGGC
                     QAEIGVSSAMAAAALTEAIGGNQRQAMMAAEIAMEHHLGLTCDPIGGLVQIPCIERNT
                     MGAIKAITASQLALQSTPDFAKVSLDAVIQTMWNTAQDMNSKYKETSDGGLAVQIPLG
                     LSEC"
BASE COUNT          579 a          824 c          784 g          614 t
ORIGIN      
        1 gtccgtatcg ataacggttt cgatctcaaa gtgggcggcg tctacgaaga cctgccgcgc
       61 aataccagat tctatgagac cgcctgcctg ctgccctggg aaaaccaggc gaactggctg
      121 gcctccaaag acccgcaatc gaactggtac aactcgtgcg gtttcctgta cgtccagctc
      181 aaagacaatg tcaccatgga tgaggcggcc acctctgtaa gaaatgcccc caccgaacat
      241 atccgggagt acgaagaaca cgccacgctt tatccattgg acaagctcca tctgtatact
      301 gattttacca atggcacgat gtcgggcggc cgcattcaaa cgatccggat gttcggcatc
      361 atcggggcct ttgtcctgct gctggcctgt atcaatttca tgaacctaag tacggcgcgg
      421 agcgagcgcc gggcgaaaga ggtgggtatc cgtaaggcca tcggctcact gcgccggcag
      481 ttggtagctc aattcatcac cgaatcgctg gccatggctt tcctatccat gggattggcc
      541 ctcctattga cacagctggc ccttcctttt ttcaacgacc tggccgataa acaaatggcg
      601 ctcccctggg caagccccgc tttctggagc tggctcttcg ccttcacact gctcaccggg
      661 ttgatagccg gcagctatcc ggctttttac ctttcaggct tccggccaat aaaggtctta
      721 aagggaaccg caaggttccg accgaggacg agcgtgtcga agcagctcgg ccgatggtca
      781 ctacgtgtgg gacgttgggc ggcgctcccg cggcgggcgc tcgtcgttgt gcaatttacc
      841 atttccatgg cgatgatcat tgggaccatc atcgtcttcc gccagatccg gtacgccaag
      901 gacagagccg tgggctatac tcgcgaaggg ctgatcgccg tgaatatgaa cacaggcgat
      961 ctgtacgcgc atagcgaggc catccgcgcc acgttgctgc aaacaggcgc ggttacggat
     1021 atgggcgaat ccaatagcct gcccaccgaa atatcttcag gaaatgacgg gttttactgg
     1081 aaaggcgtgg agcccggtaa ctcctacggt ttcgggacca tcggcgtaga cttcgacttc
     1141 ggccacacta tcggctggca ggtgaccgaa ggccgtgatt tctcccggca tttcccggcc
     1201 gatacggctc gcaatagggg cggcctcatc gtgaacgaag ccgcggtgaa acagatgggg
     1261 ctaaaacatc ccgtcggcga gatcgtgcat tacaaaggta ccatctttcc gaatgtggat
     1321 catgtcatcg tgggggtggt gaaagatatg gtgatggaat cgccttacga aaagccgaga
     1381 cccgtctttt tcctttgcgg cggctgggtc aactatatgg tcatacgcat cgatcctgct
     1441 atgccggtgc gggacgcatt ggcaaagatc gaaccggtct tcaaacaata caatcccggt
     1501 agccccttcg aattccattt tatcgatgac acctatgcga agaaatttgc cgatgaggaa
     1561 cgcatcggcc ggctggcggg tgtctttgcc gcattggcca tattcatctc ctgtctgggc
     1621 ttattcggcc tcgcctcctt catggcggaa cagcgcgtca aggagatcag cgtgcgtaag
     1681 gtcctggggg cgaccatttt caacctctgg cagctactgt cgaaagactt tatcgtcctg
     1741 gtgctgttgg cctgctgttt ctccattccc ctggcctggt atctgctaca cggctggctg
     1801 caacattatg attaccggac gaacatcagc gcctggatct ttgtggccgt aggcgccggc
     1861 gcgttggcga tcacgctgtt gacggtgagc tggcaggcca ttagagccgc attggccaat
     1921 ccggcgaaac atctccgcac ggaataggga tcctggtttc agcattcgct caatcccaac
     1981 ggtatctgca ccgccagccc gccgtcggag gtctccttat acttactatt catgtcttgc
     2041 gccgtattcc acatcgtctg gatcaccgca tcgagcgaga ccttggcgaa atcgggcgtg
     2101 ctttgcagcg ccagctgcga ggcggtaata gccttgatgg cgcccatcgt gttgcgttcg
     2161 atacagggga tctgcacgag accgccgatc ggatcgcagg tgagaccgag atggtgttcc
     2221 atggctattt cggccgccat catggcctga cgctggttgc cgccgatggc ttcggtgagg
     2281 gctgcggcag ccatggcgga gctaacgcct atctcggcct gacagccacc catggccgcg
     2341 gaaatggtgg cccctttctt aaagatgctc ccgatctccg atgcggtcag gatgaaccgg
     2401 tagatctttt cttcgttata gctatcgcag aagatcacaa aataatgcag gaccgccggg
     2461 atgacgcctg cagcgccatt ggtaggagca gtcacgacac ggccgaacga tgcgttctcc
     2521 tcgtttaccg ccagggcaaa acagctaacc cagtcaagcg tatacttgaa tccgttgcca
     2581 cccgagcgga tggcctgtac ccaggagtcg caatcatgat aggaagaatc gccgatcagc
     2641 cggcggctga gtgccgcggc acgacgttga acatgcagcc cgccgggcag ctgcccctcg
     2701 gcatgacaac cccggtaaac gcaatcccgc atgacgttcc atatccttcc cacgcccgcg
     2761 cgggtctccg cctccggccg ccaggccgcc tcattttcca t
//
LOCUS       DATGKE010000354         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_756566, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000354 DATGKE010000000
VERSION     DATGKE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756566"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="VKU83_06095"
     CDS             complement(<1..1618)
                     /locus_tag="VKU83_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HLI93157.1"
                     /translation="MNGNTTPTLKRQIFQTNSTGRWQRFKWVGRILLFLLILGVVIFY
                     ITIKRNYAPEMPRIKEQSQLYKKVLDTGRTFLFKNSLIEQYGGFRKYINEKVPYHGGA
                     FPGQRHDTDRAHMTPVKVDTSFRSFTKFPTGIRAAFYVDWDAQSFLSLQQYIDKMNLV
                     IPEWIFLDPTGDTVTTNIDPRAWSVMKKSGVKIMPILSNNYKGDFRGDVVHRVINDPV
                     KMQRLITDVISILQKDNFIGVNVDFEELQETNNEKLVEFQKDLYEQLHAQHLLVTQDV
                     IPFNDDYNFKELSRYNDYVFLMAYDQYSDASGPGPIAHQKWIEGAVDAAVKNIPIQKL
                     ILCMAAYGYDWRLGKDLSARTVSYQEALSTAKDQEATIDFDNDSYNLHYDYDDDDGVR
                     HQVQFTDAATMFNELRFATEYGLPGTAIWRLGDEDPRVWSFYDEDMRKSAVAHFDFTA
                     FSHVDAKSNADFVDYEGSGEVLDIIATPTNGRITPEVNTGEMLISEETYDSLPSRFVA
                     RKYGTEDSMKLVLTFDDGPDPEYTPKILDTL"
     gene            complement(1700..3481)
                     /gene="dnaX"
                     /locus_tag="VKU83_06100"
     CDS             complement(1700..3481)
                     /gene="dnaX"
                     /locus_tag="VKU83_06100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLI93158.1"
                     /translation="MDKFIVSARKYRPQDFSTVVGQSHVTTTLKNAIKNGQLAHAFLF
                     CGPRGVGKTTCARILAKTINCENVSPEGEACNRCHSCISFNEGTSLNIHELDAASNNS
                     VDDIRSLVEQVRFTPQAGKYKVYIIDEVHMLSASAFNAFLKTLEEPPPYAIFILATTE
                     KHKILPTILSRCQIFDFKRITATDTVDHLEEIVAKEGIRAERPALQLIAQKSEGCMRD
                     ALSILDKIISFTNGELTYRNTLEHLNILDADYYFRLLDAMQRQDLPDALLIYDDINRK
                     GFEGDLVLNGLAEFIRNLLVCKDQKAAVLLEAVESFRDRYVTVGKKVDAGWLVTALNV
                     LNEAEINYKSARNKRLHVELALIKLCYLAQAMQVVGGTQKKPAEALKSVSFRSIPAMQ
                     PAASRGAKLMIDAGAESAAAAATAPAKKPAAHAEAARQEAAQKPEALQQAAGSLGSLS
                     SIRRQFAGQAQDKDPGSGRALEAEALHQAWRQYADGLRENRNPAVQSLEWATLRITDI
                     HSFEVVTTNNLQQRFIEQEKRGISDHLQKVFANKAINFVVVVEEGTTNDNPADKPLNK
                     REQFLQIVDQYPLVKELKDRLKLELDY"
     gene            complement(3804..>4207)
                     /locus_tag="VKU83_06105"
     CDS             complement(3804..>4207)
                     /locus_tag="VKU83_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93159.1"
                     /translation="AYYGERPIAVFLNIPELNQYYKHFNGKLGWLQMLHFVWLKMRGA
                     CRHFTGIVFALIPEFQGKGVDAYIIQELANIVQPLGDYDHYEMQWIGDFNVKMVNIAR
                     SLGDTHVSRKLTTYRYLFDRTREFKRHPMLA"
BASE COUNT          854 a         1181 c         1130 g         1042 t
ORIGIN      
        1 tgagggtatc gaggatcttc ggcgtgtatt ccggatcggg accgtcgtca aaggtcagca
       61 ccagcttcat ggaatcctcg gtaccgtatt tccgcgcgac aaacctcgag ggaaggctgt
      121 cataggtctc ctcgctgatc agcatctcgc ccgtattaac ttcgggcgtg atgcggccgt
      181 tggtcggggt tgcgataata tccaggactt cgccggagcc ttcgtagtcc acaaaatcgg
      241 cgttggattt agcatccaca tggctgaatg cggtgaaatc gaagtgggcg acggccgatt
      301 tccgcatgtc ttcgtcatag aacgaccata cccgcgggtc ctcgtcgccc aaccgccaga
      361 tcgcggtccc cggcagcccg tactccgttg caaaccgcaa ttcgttgaac attgtcgccg
      421 catccgtgaa ctgcacctga tggcggacgc cgtcatcatc gtcataatcg tagtggagat
      481 tgtacgagtc gttgtcgaag tctatcgtcg cctcctggtc cttggccgtc gagagcgcct
      541 cctggtagct gacggtccgg gccgatagat cttttccgag ccgccagtca taaccgtagg
      601 cggccatgca caggatcagt ttttgaatgg gaatattctt cactgccgcg tcaacggcgc
      661 cttcgatcca tttctgatgg gcgatcggcc cggggcccga cgcatccgaa tactgatcgt
      721 aagccatcag gaagacatag tcgttgtatc tggacagctc cttgaaattg taatcgtcgt
      781 tgaagggaat gacgtcctgc gtaaccagca ggtgctgcgc atgcaactgt tcgtaaaggt
      841 ctttctggaa ttcgacgagc ttttcgttat ttgtctcctg caattcctcg aagtccacat
      901 tcacgccgat gaaattatcc ttttgcagaa tgctgatcac gtccgtgatc agccgctgca
      961 tcttaacggg gtcgttgatc acgcggtgaa cgacgtctcc cctgaagtct cccttgtaat
     1021 tgttggaaag gatcggcatg atctttacgc cggatttctt catcaccgac cacgcccgcg
     1081 gatcgatatt cgtcgtgacc gtgtcacccg tcggatcgag aaagatccat tcggggatca
     1141 cgaggttcat cttgtcgata tactgctgca gggaaaggaa ggactgcgcg tcccagtcga
     1201 cataaaaagc cgccctgatg ccggttggaa atttggtgaa cgatctgaag ctggtgtcca
     1261 ccttcaccgg cgtcatatgg gcccggtcgg tatcatgccg ctgtcccggg aaagccccgc
     1321 catgatacgg tactttttcg ttgatatatt tccggaatcc gccatactgc tcaatgaggc
     1381 tgtttttgaa aagaaaagtc cggcccgtat ccagcacttt cttgtataac tggctttgct
     1441 ccttgatgcg gggcatttca ggggcataat tacgtttgat cgtgatgtaa aatatgacga
     1501 cacccagtat cagtaaaaac aaaaggatcc gcccaaccca tttaaatcgc tgccagcgcc
     1561 ccgtggaatt cgtctggaag atctgtcgtt tgagtgttgg tgtcgtatta ccattcatag
     1621 atccgtaggt attcgcaaaa ttcccgccaa tcggcgcgct gcgccggcgg gacatgatct
     1681 tttaactaaa ctatatggct caataatcga gttccagctt cagcctgtcc ttcaattcct
     1741 tgaccaacgg atactggtcc acgatctgga ggaattgctc ccgtttgttg agcggcttgt
     1801 ctgccggatt gtcatttgtc gttccttctt ccaccacgac gacaaagttt atcgccttgt
     1861 tggcgaacac cttttgcaaa tgatcggata ttccgcgttt ttcctgctcg ataaaccgtt
     1921 gttggaggtt gttggtcgtc acgacttcaa aagaatggat atcggtgatc ctcagggttg
     1981 cccattccag gctctgcacc gccgggttcc ggttttcccg cagcccgtcg gcatattgac
     2041 gccatgcctg gtgcagtgcc tccgcttcga gcgccctgcc gcttccagga tccttgtctt
     2101 gcgcctgacc ggcaaactgc cggcggatgc tcgaaagcga accgagggag ccggccgcct
     2161 gctgaagtgc ctccggcttc tgcgctgctt cctgccgggc cgcttccgcg tgggctgcgg
     2221 gcttcttcgc cggggccgtg gcggctgccg cagcgctctc cgcgcccgca tcgatcatca
     2281 gctttgcgcc cctggacgca gccggctgca tggcggggat actccggaat gatacggatt
     2341 tcaacgcctc ggctggcttc ttctgggttc ctcctaccac ctgcatagct tgtgccagat
     2401 agcacaactt tatcagcgcc agctccacat gcaagcgttt attgcgagcc gatttgtaat
     2461 tgatctccgc ttcgttcaaa acgtttaaag ccgtcaccag ccagcccgcg tcgacttttt
     2521 tccccacggt gacatagcga tcgcggaagc tctccaccgc ctccagcagg accgccgctt
     2581 tctggtcctt acaaacgagc aggttgcgga tgaattccgc caggccgttc aacaccagat
     2641 ctccttcaaa tccctttcgg ttgatatcat catagatcaa cagcgcgtcc ggcaggtcct
     2701 ggcgctgcat cgcatccagc agccggaaat aataatcggc atccaggatg ttcaggtgtt
     2761 cgagggtgtt ccggtaggtc agctcgccat tcgtaaagct gatgatcttg tccaggatgc
     2821 tcagcgcatc ccgcatgcag ccctcgctct tctgggcgat cagctgaagc gccggtctct
     2881 ccgcacggat cccctccttc gccacgatct cctccaggtg gtcgacggta tccgttgccg
     2941 ttatccgttt aaagtcgaag atctggcaac gcgaaaggat cgtgggaagg atcttgtgct
     3001 tctcggtggt cgccaggata aagatcgcat aaggcggagg ctcttcgagg gtctttaaaa
     3061 aagcattgaa agccgaggcg ctcagcatat gcacctcgtc gatgatatag accttgtact
     3121 ttcccgcctg gggggtgaaa cgcacctgct cgaccaggct ccggatatcg tccaccgagt
     3181 tgttgctggc tgcatccagc tcatggatgt tcagggatgt gccttcattg aaggaaatac
     3241 aggaatgaca gcggttgcag gcttcgccct ccgggcttac gttttcgcaa ttgatcgtct
     3301 tggccaggat ccgggcacag gtcgttttcc ccactccccg cgggccgcag aaaaggaaag
     3361 cgtgcgccag ctggccgttc tttatggcgt ttttcagggt ggtggtgaca tgggactggc
     3421 ccactacggt cgaaaagtcc tggggccggt attttcgggc ggaaacgatg aatttgtcca
     3481 ttactttagc tcctgcggca aacctaccac aaatccctga cattagcggc cggcgccggc
     3541 tcccccggcc cccccggccc cctgggacgc gcaagcaagg gcctggaaac agatgaggtc
     3601 accatttggt gacctcaagc gtttttcaga tcttacggct caggatctgc tatccggccg
     3661 gctctacgtg taattttcag gtatcgggta tatttcacac attgtgccaa attgatgagc
     3721 attgaaaatc aaatatttat gaaaatgcct gttaccggaa tacttcccag ttccggaata
     3781 aacgccaaga atgagacggt cttctaggcc agcatcgggt ggcgcttgaa ttcccgggtc
     3841 cggtcgaaca ggtaccggta ggtggttagc ttgcggctga catgcgtgtc gcccaacgat
     3901 ctggcgatat tcaccatctt gacattgaag tccccgatcc actgcatttc gtaatggtcg
     3961 tagtccccga ggggttgtac gatgttagcc agttcctgga tgatataggc gtcgacgccc
     4021 tttccctgga attccgggat gagggcaaag acgatcccgg tgaaatgccg gcaggcgccg
     4081 cgcattttca gccagacgaa gtggagcatt tgcagccagc ccagtttacc gttgaagtgt
     4141 ttgtagtact ggttcagctc cgggatgttc agaaatactg cgatcggccg ttcgccataa
     4201 taggcga
//
LOCUS       DATGKE010000355         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_712659, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000355 DATGKE010000000
VERSION     DATGKE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712659"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VKU83_06110"
     CDS             <1..1750
                     /locus_tag="VKU83_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93160.1"
                     /translation="PSTRFAGVTPGTYDVTVKNSDGCVSSSTAAVIQAQPKPPQIPVV
                     IVTQPTCATGSGTITISGADTTSLSYSVNGAAYLAQSTFPNLTTGTYKVTAENSAGCV
                     SPAVTATVDSVPGAPAAPNVVITQPSCTVATGTITITSPIAAGYTYRIGDSAAFQPIP
                     LFNNVPPGTYYVSAKGGESGCVSLPATAVVNPSPALPKPTVSVTQATCSINTGSITVT
                     SPADTGLLYSIDGVTYQPDSVISPVSPGSYQVTVKDGAGCVSAAAAAQVLNPPSSCAA
                     CAAAQHSNFDGFLPSANPGSATTLWFNLHTSLDSGQLAKNGEYLLFTGGNLVLSGVGA
                     SFDSSVALPDGEIIADAAVTAPSTSFVSAKNLWITRVPVGYQSDDVFVSGAAINSASG
                     FTVSGPAGTSILSGSWSSNADVQSKWWYGLACYQPEFDNTTVGSVNAVDGEPVGGHIA
                     GTPNDQTSGLVAGGSGNGGSNYTGNLSTPDDLLACMVQQPVPTKVQLKVFPNPYQSTV
                     IFNINSSVSGTGTLEFYNLVGNRLSVIADLQFQAGTPQSITVPMLEIGERQPVVYLFR
                     IKGIRLQGTLLPEAFR"
     gene            complement(1917..2351)
                     /locus_tag="VKU83_06115"
     CDS             complement(1917..2351)
                     /locus_tag="VKU83_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI93161.1"
                     /translation="MSRVSIYLNFQGNTEEAFNYYKSVFKTEFTAPISRMKDVPAQPG
                     MPPLPEKEKNAVMHVDLPILGDTHLMGTDMLESMGHKLKIGNNTTICLEPDSRDETDR
                     LFKALSAGGSDIQAPQDMFWGSYWGCCLDKFGVRWMFNYTKH"
BASE COUNT          464 a          753 c          679 g          489 t
ORIGIN      
        1 gccgtcgact cgattcgccg gtgtaacgcc cggtacgtac gacgtcacgg taaagaattc
       61 cgacggttgc gtctcctctt ccaccgcggc cgtcatccag gcgcagccga aaccaccgca
      121 gatcccggtc gtcattgtca cccagcccac ctgtgcgacg gggagcggca cgatcaccat
      181 ctcgggcgcc gacacgacgt cgctcagcta tagcgttaac ggagctgcct acctcgcgca
      241 gtccaccttc cccaacctga ccacgggtac gtataaggtc acggccgaaa atagcgcggg
      301 ttgcgtctct cccgcggtta cggcaaccgt cgactcggtg cccggcgctc cggccgcgcc
      361 aaatgtggtg atcacccagc cttcctgtac ggttgcgacc gggacgatca cgatcacgtc
      421 gcctatcgcc gccggctata cctaccggat cggtgactcg gcagccttcc agcctatccc
      481 gctgttcaac aacgtgccgc cgggcacata ctatgtttcg gcaaaaggcg gtgaaagcgg
      541 ttgcgtatcg ttgcccgcta cggccgttgt caatccgtcc ccggcactgc caaagcccac
      601 ggtcagcgtg acccaggcta cgtgcagcat caatacaggg tccattaccg ttacttcgcc
      661 ggcggatacc ggccttctct atagcatcga cggcgtcacc tatcaaccgg actccgtcat
      721 cagcccggtt tcgcccggca gctaccaggt gacggtgaaa gacggcgccg gctgcgtatc
      781 cgcggcagcg gcggcgcagg tcctcaatcc gccgtccagt tgtgctgcct gtgccgccgc
      841 gcagcacagc aatttcgacg gcttcctgcc gtccgcgaac cccggttctg cgacgacgtt
      901 atggtttaac ctgcatacga gtctcgacag cggtcagctg gcaaagaacg gcgagtacct
      961 gctctttacg ggcggcaacc tggtgctttc gggagtcggc gcatccttcg actccagtgt
     1021 tgcgctgccg gacggagaga tcattgcaga cgccgctgtg acggcgcctt ccacttcttt
     1081 tgtgtcggcg aagaacctct ggataacgcg ggtacccgtg gggtatcagt cggatgacgt
     1141 ttttgtgtcc ggcgcagcca tcaattcggc ttccgggttc accgtctccg gacctgcggg
     1201 cacatcgatc ctttcgggca gctggtcgag caatgcggat gtccaatcca aatggtggta
     1261 cggcctggct tgttaccagc cggaattcga taataccacg gtaggcagcg tcaacgcggt
     1321 cgacggcgag cccgtcggcg gacatatcgc cgggacgccg aacgatcaga ccagtggcct
     1381 tgtggccggc ggcagcggca acggtggctc caactatacc ggaaacctga gcacgccgga
     1441 tgacctcctg gcctgcatgg tacaacagcc ggttccgacg aaagtgcagt tgaaggtctt
     1501 cccgaacccg taccaatcca cggtcatttt caacatcaac agctccgttt ccggcaccgg
     1561 cacactggag ttctacaacc tggtaggaaa taggctgagc gtcatcgccg acctgcagtt
     1621 ccaggcggga acgccccaaa gcatcacggt gcccatgcta gagattggcg aacggcagcc
     1681 ggtggtctac ctgttccgga tcaaaggaat ccggctgcag ggaacactgc tgcccgaggc
     1741 gttcaggtga cgcatgagat tgctgccggt tatcgacagg tttctcagcc agccggccga
     1801 taaactgccc tttgcaacaa cagaaattcc ctatgagcga ggcgtgtcaa gatagccacg
     1861 ccggccccgg aagccagccg ggtcggcccc ggccccgcaa cgccggatca accggttcaa
     1921 tgtttcgtgt aattgaacat ccagcggacg ccaaatttat ccagacagca gccccagtac
     1981 gaaccccaga acatatcctg gggcgcctgg atgtcggagc cccctgcgga caaggctttg
     2041 aacaaccggt ccgtctcatc ccggctgtcc ggttccaggc agatggtcgt attgtttccg
     2101 attttgagtt tgtggcccat cgattccagc atgtcggttc ccataaggtg ggtatcgccg
     2161 aggatgggca ggtcgacatg catcaccgca ttcttctcct tctccgggag cggcggcatc
     2221 ccgggttgcg ccgggacgtc cttcatccgg gaaatcggcg cggtaaattc ggtcttgaaa
     2281 accgatttgt aatagttgaa cgcttcttcg gtgtttccct gaaaattcag gtagatggat
     2341 acgcgtgaca tagcttttta ttttgggtaa agctcatcct ttttt
//
LOCUS       DATGKE010000356         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_435323, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000356 DATGKE010000000
VERSION     DATGKE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435323"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VKU83_06120"
     CDS             complement(<1..702)
                     /locus_tag="VKU83_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93162.1"
                     /translation="MEKILLAIDSLNLDENAVHFAAYLARLTHSNLTAIFLQDLVRQE
                     AVVLQLGEDGNAGEVIMIQDSADEGAMELRDENIQLFRELVREAGIPAGVYLDNGDPA
                     TGIIAESRYADLLVIDPSTSFSARYDGPPTRFVKDILQEAGCPVVISPKSFEDIDNII
                     FCYDASKSSLFAMKQFSYLFPELRSKRAKVIDLVTGEFPGDKEGVIEWLKYHYDDAEW
                     IAAEPNVTDALFNYLL"
     gene            846..1184
                     /locus_tag="VKU83_06125"
     CDS             846..1184
                     /locus_tag="VKU83_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93163.1"
                     /translation="MRKAKTPRTDALFGVFLQDIGAIRGKHPRSFSKKYFRFDYAENE
                     KEGYSLSIEPDSFPKAVKKEIIRAFDKRLNQDLLPAIGQLKRNRRAIKREKLSGMQTF
                     VAMKNEIKQT"
     gene            1188..1673
                     /locus_tag="VKU83_06130"
     CDS             1188..1673
                     /locus_tag="VKU83_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLI93164.1"
                     /translation="MKQTIQAEADTTASSCSTLNIGSEAVGKDGFILSQYTCVGANIT
                     PPLDIGGIPKDAKSLAIIMDDPDAFSGNWVHWIAWNIPVLSHLKEARQMEEQGMNDLH
                     QKRYDGPCPPHGRPHHYHFKVYALDSLLHLSGNTTKSELEQAMEGHILAFGEMVALYK
                     R"
     gene            complement(1694..2935)
                     /locus_tag="VKU83_06135"
     CDS             complement(1694..2935)
                     /locus_tag="VKU83_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLI93165.1"
                     /translation="MTSTGQGSQLMAGSTMDSRQFEALFNNAIIGIVLTDQAGEIMLV
                     NQFAETQFGYAASELIGKQVEVLIPNEFRKGHEGHRQGFYRHPQTRVMGAGRDLYALR
                     KDGTEFPAEVSLSYYEFEGKNYVMALIVDITLRKEGEQLLFRHNEELEKKVAERTLRL
                     QEAVLVLKQSDAELQTLYEREKALGEIKSRFVAMASHEFRTPLSTILSSISLVKRYSS
                     PEDIPGRERHISRIQSAVKTLNSILEDFLSLGKLEEGLINHNPARLDKRSLLSDIDEL
                     LQEMNEVSGKNQKMELSDSLTTDFFTLDKKLLRYILMNLISNAIKYSPEKSIIAIHCR
                     DEAGDLQITVRDNGIGIPEKEQEHLFERFFRADNAGGIPGTGLGLHIVRRYLDLMGGR
                     IALSSKLNEGTTFTLFVPQPG"
     gene            3502..>3833
                     /locus_tag="VKU83_06140"
     CDS             3502..>3833
                     /locus_tag="VKU83_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HLI93166.1"
                     /translation="MSKNRRTRTPSMWWMVGMIALITIFPYICRSVSPSTPKQINWSE
                     FELRMLDRHAVDSIEVVNNEYADVYVRKQFADDPYFRNITHPVAGKPMEPGPHYRFNI
                     GSVDLLER"
BASE COUNT         1040 a          954 c          884 g          955 t
ORIGIN      
        1 gagcaggtag ttgaacaggg cgtccgttac gttcggctcg gcggcaatcc attcggcatc
       61 atcataatga tacttcagcc actcgatgac tccctctttg tctcccggga attctccggt
      121 gaccagatcg atcaccttcg cccttttcga tctcagctcg ggaaacaggt aggagaattg
      181 tttcatcgca aataacgagg atttgctcgc gtcatagcaa aaaatgatat tatcgatatc
      241 ctcaaagctt ttgggagaga tgaccaccgg gcatccggcc tcctgcagaa tatccttcac
      301 aaagcgggtc ggcgggccat cataccgcgc tgaaaaggat gtggagggat caatcaccag
      361 cagatcggcg tacctgcttt ctgcaataat accggtggcc ggatcaccgt tatccaggta
      421 gacgccagcg gggatccccg cttcccttac caattctctg aaaagctgga tattctcatc
      481 ccttagctcc atagcgccct catcggcgct atcctggatc ataatcacct ctccggcgtt
      541 gccgtcctca cctagctgaa ggacaaccgc ctcctgccgg accaggtcct gcaggaagat
      601 tgccgttaaa ttcgaatggg tcagacgcgc caggtaagcc gcgaaatgga cggcattttc
      661 atccaaattg aggctgtcga tcgccaaaag tattttttcc atactaattt ttcataaagc
      721 tatcctcaaa tcgccttcct tactatgacg gccgccaacc gcaaagctga ttaggatcag
      781 gtacaaccct ttccttgatc ctttttcccg gaataacggg atggtaactt gagaaaaaga
      841 aagcaatgag aaaggcaaaa acaccccgaa cagatgcgct ttttggggtt ttccttcagg
      901 atataggagc tataagagga aagcatcccc gatctttcag caaaaaatac tttcgattcg
      961 actacgccga aaacgaaaaa gagggctaca gcctgtctat tgagccggac agtttcccaa
     1021 aagctgtaaa aaaggaaatc atccgggcgt ttgacaaacg tctcaatcag gacttacttc
     1081 cggcgatcgg ccagcttaag aggaaccgta gagcaatcaa gcgagaaaag ttgtctggta
     1141 tgcagacctt cgtggctatg aagaatgaga tcaaacaaac ctaaaaaatg aaacaaacaa
     1201 ttcaggccga agcggacacg actgcatcct catgcagcac cctaaatatc ggtagcgagg
     1261 cggtcggcaa ggatggattt atcctatccc aatacacttg tgttggagcg aatataactc
     1321 cgcctttaga tatcggcgga attccgaaag atgcaaaaag tctggccatt attatggatg
     1381 atccggatgc tttctcgggc aattgggtcc attggattgc atggaatatt cccgtgctca
     1441 gccaccttaa ggaggcaagg caaatggagg agcaaggcat gaacgacctg catcaaaaaa
     1501 ggtatgatgg accatgccct ccgcatggca ggccacacca ttatcacttt aaggtgtatg
     1561 ctttggatag tttgctccac ttgtccggca acacgaccaa aagtgaactt gagcaggcga
     1621 tggaggggca catcctggct tttggggaga tggtcgcgtt atataaaaga tgaactcact
     1681 cgctgaatcg gcactagccc ggctgtggta caaataatgt gaacgtagtc ccttcattta
     1741 gcttactgct caatgcaatg cggccgccca tcaaatccag gtatctgcgt acgatatgca
     1801 ggccgagccc tgtgccaggg atcccccctg cgttgtcagc acgaaagaac cgttcaaaga
     1861 ggtgttcctg ctccttttcc ggaatgccga tcccattatc ccggaccgtt atttgcaaat
     1921 caccggcttc atcgcggcaa tgaatcgcta taatggactt ctcaggggaa tatttgatgg
     1981 cattggatat caggttcatc aggatgtatc gaaggagttt tttgtccaac gtaaaaaaat
     2041 cggtcgttag tgaatcggaa agttccattt tttgattttt gccggagact tcgttcattt
     2101 cctgcaaaag ttcatcgata tcggagagca atgagcgttt gtccaatcgc gccgggttat
     2161 ggtttatgag cccttcttcc agctttccca acgacaggaa atcctcaagt atggaattta
     2221 acgttttaac ggcactttga attcttgaga tatgccgttc ccgaccaggt atgtcttccg
     2281 gcgatgagta tcgtttaaca aggctgatgg atgaaaggat agtactcaac ggtgtccgga
     2341 attcatgcga ggccatagct acaaagcggg actttatttc tcccagcgct ttttcccttt
     2401 catacaatgt ctgcagctct gcgtccgatt gtttcaacac cagaaccgcc tcctgtagcc
     2461 ttaacgtacg ttcagctact tttttttcta attcctcatt gtgccggaac aacaattgtt
     2521 caccttcttt cctcagcgta atgtccacga tcagcgccat gacataattt ttgccttcaa
     2581 attcatagta actaagactc acttccgcgg gaaactcagt tccgtccttc cgaagggcat
     2641 atagatcccg gcccgccccc atgacacggg tttgtggatg acggtagaac ccctgccggt
     2701 gcccctcatg ccccttccgg aattcatttg gaattaacac ttccacttgc ttcccgatca
     2761 attcactcgc ggcataacca aactgggttt cggcaaactg gtttaccaac atgatctccc
     2821 cggcctggtc ggtcagcact atgcctatga tggcattatt gaaaagtgcc tcaaattgac
     2881 gggaatccat ggtcgacccc gccattagtt gagatccttg accagtactt gtcattccgt
     2941 tcgattttag atcacaaaag gtttagatac ctacctaact acatcaatta caataatcgc
     3001 agagtatgat ttaaggaggg atttctttta aaaaaaagta ttgaacaaag ccgatgataa
     3061 atgcaataag agaaacagtg ctgactcagg taattgctag atcaaataaa cctatactgt
     3121 ctaaatttac acctattctt tggataacta gcattaataa acacttatgg gttccaggag
     3181 agctaaatcg ctatgaatga ccacaatcat cgccgtcagt gatgggcaat acccagccct
     3241 gagcaggcgg ccgcccaatg ccggcctttt tttggaagaa tgcctgacga atgtcaatcc
     3301 gtgacttgac ggaagtccaa gtcctgagca acatgcgggg ctaactttaa aaacggggaa
     3361 tggaaaaaac aagaaatcgg aaattcataa ctataagtca aaacatctcg tttatggaat
     3421 ccctgcacat gaccggtcct aaattcaggg aaatgagcgt ggctgaatat gggctatttt
     3481 cttcagaaag ggaggtaacc cgtgagtaag aaccggcgca cgcggacgcc atcgatgtgg
     3541 tggatggtcg gaatgattgc actgataacg atcttccctt acatatgccg cagcgtttcg
     3601 cccagcactc ccaaacagat caactggagt gaattcgagt tgcgcatgct cgaccgccat
     3661 gcggtcgaca gcatcgaagt ggtaaacaat gaatatgctg acgtatatgt ccggaagcaa
     3721 tttgcggatg acccgtactt ccggaatatc acccacccgg ttgcaggcaa gccaatggaa
     3781 ccggggccgc attaccgctt caatatcggg tcggtggacc tcctggaaag gaa
//
LOCUS       DATGKE010000357         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_215039, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000357 DATGKE010000000
VERSION     DATGKE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215039"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VKU83_06145"
     CDS             <1..720
                     /locus_tag="VKU83_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLI93167.1"
                     /translation="DLAALRSLVSDRTKYISVTTPHNPTGVCLGLAQLRQLADLAEER
                     GIYLLVDETYRDMVFGEQLPLAATLSPRVISVSSLSKTYGLPGLRIGWLVCGDPQLLG
                     KLLAAKEQMHICGPVIDEEIAYRYWQHRDRYLPGIRADIQLKFGLVKEWMRQQNDFEW
                     VEPQGGCVCFPRLLRPQEGDLARFYCLLLKQYGTYVAPGHWFDFPEHYLRIGFGWPPE
                     DRLTAGLTSLTSAWKSTVSVH"
     gene            851..2665
                     /locus_tag="VKU83_06150"
     CDS             851..2665
                     /locus_tag="VKU83_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93168.1"
                     /translation="MKKAVYMILGSLIGLCSASSAQIQTSASYIDVSQPGGGPIQTGD
                     ILEIRGVISVPSGTTITGLTYTSTVPGSTLYQTGTLTAETNEGVVVGGIANTGNYTDA
                     AGDDRGQIVGSAVTIYMGTGATSAAGGTVIGGTTTPVFYSSQSILMAAYRVKVTGAAG
                     ATFIVNGVMKYSVGGVPTTSTLTSLIVGVYNKSTCMTPGAINYLTVATNGSFGSGNKQ
                     NASALAQVSGFTFVNLSANQPADGYYSVVNNTSPTQYTGTTPTAGERVFGVWDVIGDH
                     TGSSNGTGNSPTANHATGGYMLAINGTYAPSAFFSTTVSGLSTNTDYTVSFWVYNLCS
                     KCGADPSTGTSSGTPGVLPNIAFAISNTDYYNTGQLAYTGQWIQSNFTFNSGAGTSFP
                     IIIRNNAPGGGGNDFAIDDISLSTCLTLLPVTLINFQGNQEPGGAVLDWQTSREENTA
                     YYVVERNTGDDKFDSVGRVTSHLLAAGSAYHFTDNNAPQVKAIQYRLRMVDIDGNFTY
                     SPIVVVGGAALPAQTINLAPNPSHTAATLYMEAEAPGTVQISLWSLAGARVLAQTNTV
                     SKGQNALPIESLGRLAKGMYVVRMISGNWSASTKLVVE"
     gene            complement(2685..3080)
                     /locus_tag="VKU83_06155"
     CDS             complement(2685..3080)
                     /locus_tag="VKU83_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLI93169.1"
                     /translation="MDNTRTDKTFNFNPPIDNQYLIELYAGDYSMIGETLSEVLLVYD
                     DFVENVCSGFERGDLLSLKGAVHKLKPLFGFVGLPCLEAQCRTFESNCMLADSKTLAI
                     EFNQLKDSILSIKSFLETEKARLEAFNKR"
     gene            3182..5317
                     /locus_tag="VKU83_06160"
     CDS             3182..5317
                     /locus_tag="VKU83_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI93170.1"
                     /translation="MRNPAVSLIRSFYEDIVYSGIREDYSPSKRKNTVRFNQFILLIL
                     IAHFVCVISYFFFGLYISALINLTAAYIFIVAYHLGLRQKFLLARIVSVINLNLYLVI
                     MNYMEGVKAGEYLFYFPYFLIMTFIINFRRKYVELMVAYLITLIALLCCYYLCPPENY
                     YQVHIAGMFDKLYESNLVLSLLLSTFFSYAILRINRNNESTILKEKQFVDTIFNTSLD
                     GVFILDAVAMIITDCNQRALELLEIDHKAQIVGAPVDSLFTKEHLGLFGHVGNHLDDP
                     HSNSWQGELALASRKDNAIFAFVSVVSFLDNGRRFWKISVLDISEIKMTQFELMKAKQ
                     KAESASRAKSRFLSNMSHELRTPLNGIIGSSNLLLQEKFLPEQRSHLEILKFSSEHMM
                     VLINDILDFNKIESGRFELESLPVNLRAFLQKLEAQFSVQAHARGLLFLLDIDESLEA
                     EFLTDETRLNQIFSNLLSNAIKFTHTGGITLGARKISSTSKRSAIQFMVKDTGIGIPA
                     NKQHEVFESFTQADTDTTRKYGGTGLGLAISRKLVGLFGGDLQLQSEEGKGSTFYFTI
                     ELEVNENAKLYINDAKVKQLAGFDRLRVLIAEDNSVNMSVVRRFLVKWGIEVHEATNG
                     IEAVEKCRADSYDLVLIDLEMPEMDGATALAEIRKLHPSVPAIAFTAAVYEDMKVDLV
                     KKGFMEVVPKPFRPEDLHSKIQKLVSARA"
     gene            5380..5925
                     /locus_tag="VKU83_06165"
     CDS             5380..5925
                     /locus_tag="VKU83_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93171.1"
                     /translation="MEPPSWPVRFNRLRQLFWFFFFITVVYMVWVRVYLFPLSSDELI
                     QFEIAKTADKAAAIIRDWKNAGKYELSVQSTYFAYIFMVLYTVAIALGCRFLSACTGN
                     LIMIKGGKGFSWLIVGATACDLVENMALSRTIRGQISQWNVTLAYDLARVKFSIVIIC
                     ILFMLASALYWGIGRLAREEA"
     gene            complement(5922..>6146)
                     /locus_tag="VKU83_06170"
     CDS             complement(5922..>6146)
                     /locus_tag="VKU83_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93172.1"
                     /translation="VEMYSSYDLPALQQYGKTLIGGADFYQYDAGRVNTHDTDFPLNG
                     MLMSHYRILNTNIRGDTSLHKLRYSLISPR"
BASE COUNT         1414 a         1680 c         1659 g         1393 t
ORIGIN      
        1 gatctggcag ctttgcggtc actggtcagc gatcgcacga agtatatctc ggtcaccacg
       61 ccgcacaatc cgacgggagt gtgcctgggc ctggcccaac tccggcagct ggcggacctc
      121 gccgaagagc gcggcatcta cctgttggtc gatgagacct accgcgatat ggtattcggg
      181 gagcagcttc ccctcgcggc gacgctgtcg ccgcgggtca tcagcgtctc gtccctgtca
      241 aaaacctatg ggttaccggg cctgcgcatc ggatggctgg tttgcggcga cccgcaactg
      301 cttggaaagc tgctggcggc aaaggagcag atgcatatct gcggcccggt gatcgacgaa
      361 gagatcgcct atcgctactg gcagcaccgg gaccgctacc ttccgggcat cagggccgat
      421 atccaactga aattcggatt ggtgaaggaa tggatgcggc aacaaaacga ttttgagtgg
      481 gtcgagcctc agggcggctg cgtctgcttt ccaaggctgc tgcggccgca agaaggagac
      541 ctcgcccgct tctattgtct ccttttgaaa caatatggta cctacgtggc gccggggcat
      601 tggtttgact tcccggagca ttacctgcgg ataggatttg ggtggccgcc ggaggacaga
      661 ttgacagcgg ggctgacaag ccttacaagc gcttggaaat caactgtttc ggtccattag
      721 ccaaccctgg aaaggaattc ccaaaaatgg aagatttttt taatgtgagc gcagtaatat
      781 cctaggcatc aggccgttat atcctggcac ccttgttggc ttacgaagcc agatccctaa
      841 atttatccta atgaagaagg ccgtctatat gatcttgggt agcttgattg gtttgtgttc
      901 cgcaagctct gctcagatcc aaacatcagc ttcttacatt gacgtcagcc agcccggtgg
      961 cggcccgatc cagaccgggg atatcctgga gatccggggc gtgatcagcg ttccgagcgg
     1021 gaccaccata accggtctga cgtataccag cacggtgccg ggcagcaccc tttatcaaac
     1081 cggcaccctg acagcagaga ccaatgaagg cgttgtggta ggtggaatcg ccaataccgg
     1141 caactatacg gacgccgccg gtgatgaccg gggccagatt gtggggagtg ccgtcacgat
     1201 ctatatgggt acgggagcta cctcggctgc gggcggaacg gtgatcggcg gcacaacaac
     1261 ccccgtattt tacagttcgc agtctatcct catggcggcc taccgggtaa aagttacggg
     1321 ggcggccggc gccaccttta tcgtgaacgg tgtcatgaaa tattctgtcg gcggcgttcc
     1381 gacgacctca accctgactt cgctgatcgt cggggtgtat aataaatcca cctgtatgac
     1441 ccctggagcg atcaattacc tgacggttgc gaccaatggt agttttggct ccggcaacaa
     1501 gcaaaacgcc tccgctcttg cccaggtttc cggctttact tttgtcaacc ttagcgcgaa
     1561 ccaacccgcg gacggctact attcggtggt aaacaacacc agtccgacgc aatataccgg
     1621 aacgaccccc accgcgggag aacgcgtttt cggcgtgtgg gatgtgatcg gcgaccatac
     1681 cggttcttcc aacgggactg gcaattcgcc gaccgccaac cacgccaccg gcgggtatat
     1741 gctggccatc aacggcacct atgctccgtc cgccttcttt agcacaacgg tgagcgggtt
     1801 gagcaccaac accgattaca ccgtttcttt ttgggtgtat aacctctgtt cgaaatgcgg
     1861 cgccgatccc agcacgggta cttcatcagg gacgccgggc gtcttaccca atatcgcctt
     1921 tgcgatcagc aataccgact attacaatac cgggcaactg gcgtataccg gccaatggat
     1981 tcagagcaac tttacattca attcgggcgc cggcacctcg tttccgatca tcatccggaa
     2041 caatgccccg ggcggaggcg ggaatgactt tgccatcgac gacatcagct tatccacctg
     2101 cctgaccctt ttacccgtca ccctcataaa ctttcaaggc aaccaggaac ctgggggcgc
     2161 agtcctggac tggcaaacct cccgtgaaga aaatacggcc tattatgtcg tggagcgaaa
     2221 cacgggcgac gataaatttg acagcgtcgg ccgggtaaca tcgcatttgc tcgctgcagg
     2281 cagcgcctat cactttacgg acaacaacgc cccgcaggtg aaggcgatcc aataccggtt
     2341 acgcatggtc gatatagacg gcaatttcac ctatagcccg atcgtcgtag tgggcggcgc
     2401 agcgctgccg gcgcaaacga ttaacctggc gcctaacccg tcccacactg ccgccaccct
     2461 ctacatggaa gcggaagcgc cgggaacggt ccagatcagc ttgtggagcc tggcgggcgc
     2521 cagagtcctg gcgcagacca atacggtatc gaaaggccag aatgccctgc cgatcgaatc
     2581 gctcggccga ctcgctaaag gaatgtatgt cgtgagaatg atatccggta actggagcgc
     2641 atctacaaaa ttggtggtcg aatagttgcc gcgggtgccg cgggttaacg cttattgaag
     2701 gcctccagcc tggccttttc tgtctccaag aacgatttga tgctcaatat gctatctttc
     2761 agctggttaa attcgatcgc taaagtcttg gaatcggcaa gcatgcaatt gctttcgaat
     2821 gtccggcatt gcgcctccag acaaggcagg ccgacaaacc cgaacagcgg cttgagtttg
     2881 tgtaccgcac ccttcaggga taacagatcc ccgcgttcga agccggaaca aacgttctca
     2941 acgaaatcgt cataaaccag caagacctcg cttagcgtct caccgatcat ggaataatcg
     3001 cctgcgtata gctcgatcag atactggtta tcgatgggag ggttgaaatt gaacgtctta
     3061 tcggttcgtg tattgtccat tcatcacgct aatagatagt taacgcggct taaaataaat
     3121 tattgtcttt cagcgtttca agtgggcgac agtacgcaaa accacaaaat ccagattgcc
     3181 tttgaggaac cccgccgtct cgctgatccg gtccttctat gaggatatcg tctattccgg
     3241 gatccgggag gattactccc cttccaagcg aaagaacacg gtccgtttta atcagttcat
     3301 cctcctcatc ctcatcgcgc atttcgtctg tgttatctcc tatttctttt tcggcctcta
     3361 tatcagcgcc ctgataaatc tcaccgcggc ctacatcttt atcgtagcgt accatctcgg
     3421 cctgcggcaa aaattcctgc tcgccaggat cgtttcggtg atcaacctca acctgtacct
     3481 cgtgatcatg aattatatgg aaggagtaaa agcgggcgaa tacctcttct attttcccta
     3541 cttcctgatc atgaccttta tcatcaactt ccggcgaaaa tacgtcgagc tgatggtggc
     3601 gtacctgatc accctgatcg cactcctatg ctgctattat ctctgcccac cggagaacta
     3661 ctaccaggtt catatcgcgg gcatgttcga taagttatat gaaagcaacc tggtcctttc
     3721 gctcttgttg tccacatttt tttcatacgc catcttgcgg atcaaccgga acaatgagtc
     3781 gacgatcctt aaagaaaagc aattcgtcga tacgatcttc aacacctccc tcgacggcgt
     3841 tttcatcctg gacgcggtgg ctatgatcat tacggactgc aaccagcgcg cattggagct
     3901 gctcgagata gaccataaag cgcaaattgt cggggcgccc gtggacagcc tgttcacaaa
     3961 agagcacctc ggcctcttcg gacatgtggg taaccatctg gatgacccgc actcgaacag
     4021 ctggcagggt gaactggcgc ttgcctcccg gaaggacaac gctattttcg cgtttgtcag
     4081 cgtcgtcagt ttccttgaca acggccggcg tttctggaag atcagcgtgt tggacatttc
     4141 cgagatcaaa atgacgcaat ttgagctgat gaaggcgaag caaaaggcgg agtcggcgtc
     4201 gcgcgcaaag tcccgtttcc tgtcgaacat gagccatgaa ctgaggacgc ccctgaacgg
     4261 gatcatcggt tcctcgaatc tcctgttgca ggagaaattt ttgcccgagc aacggtcgca
     4321 cctcgagatc ctgaagtttt cctccgagca tatgatggtg ctgatcaacg atatccttga
     4381 cttcaacaag atcgagtcgg gcaggttcga gctcgaaagc cttccggtca acctgagggc
     4441 ttttctccaa aagctcgagg cccagttttc ggtccaggcg cacgcccggg gcctgctctt
     4501 cctgctggat atcgacgaaa gcctggaagc cgaattcctg acagatgaaa cgcggctgaa
     4561 ccagatcttc agtaacctgc tttccaacgc gatcaaattc acccatacgg gaggcattac
     4621 acttggcgcg cggaaaattt cgtccacgag caaaagatcg gcaatccagt ttatggtcaa
     4681 ggatacgggc atcggcatcc cggccaataa acagcatgag gtattcgaga gcttcaccca
     4741 ggccgacacc gacacgacgc gcaagtacgg cggaacgggg ttgggcctgg ccatcagccg
     4801 aaagttggtt ggtcttttcg ggggtgacct ccaactgcag agcgaagagg gaaagggcag
     4861 caccttctat tttacgatcg agctggaagt gaacgagaac gccaaattgt atatcaacga
     4921 cgcgaaagta aagcagctgg cgggtttcga ccggctgcgc gttttgattg ccgaagacaa
     4981 cagcgtcaat atgtccgtcg tgcgtcgttt cctcgtcaaa tggggcatcg aggtccatga
     5041 ggcgacaaac gggatcgaag cggtggagaa gtgccgggcg gacagctacg acctggtcct
     5101 gatcgacctc gaaatgcccg agatggatgg agcgacggcg ttggccgaga tccggaagct
     5161 gcatccaagc gtaccggcga tcgcctttac ggctgcggta tacgaggata tgaaggtcga
     5221 cctcgtgaaa aaaggattca tggaagtggt gcccaaaccg ttccggccgg aagacctgca
     5281 cagcaagatc cagaaactgg tgtcggcccg ggcgtaaaaa taatccccga aaatagcgcc
     5341 gtttatttaa cttaacaggc cgaagacaaa ttgtttaaca tggaacctcc ctcatggccc
     5401 gtgcgattca accgtctgcg acagctgttt tggttcttct ttttcattac tgtcgtctat
     5461 atggtctggg tccgggtcta ccttttcccg ctaagctccg atgaattgat ccagtttgag
     5521 attgcgaaga ccgccgataa ggcggcggcc atcatccggg actggaagaa tgccgggaag
     5581 tatgaattga gcgtgcagag cacctatttt gcctacattt tcatggtcct gtacacggtt
     5641 gcaatcgcgc tcggttgccg gttcctctcc gcgtgtacgg gcaacctgat catgatcaag
     5701 ggtggaaagg ggttttcctg gctgatcgtt ggggcgacag cctgtgatct cgtagagaat
     5761 atggcgcttt cccggacgat cagggggcag atctcacagt ggaatgtgac attggcctac
     5821 gacctggcaa gggtcaaatt ctcgatcgtg atcatctgca tcctcttcat gcttgcgagc
     5881 gcactttact gggggatcgg aagactggcc agggaagaag cctaacgagg gctgatcagg
     5941 gaataacgga gcttatgcag ggaggtgtcg ccccggatat tcgtgttcag gatacggtag
     6001 tggctcatca acatgccgtt cagggggaaa tccgtgtcgt gcgtattgac ccggcccgca
     6061 tcgtactggt agaaatcggc tccacctatc agggttttcc catattgttg caaggccggg
     6121 aggtcatagg aactatacat ttccac
//
LOCUS       DATGKE010000358         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_144530, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000358 DATGKE010000000
VERSION     DATGKE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144530"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VKU83_06175"
     CDS             complement(<1..1317)
                     /locus_tag="VKU83_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93173.1"
                     /translation="MKKLLIILIILMAVGAKGQGKKPTGPRHLHVRMLAKSYSDSIVV
                     RWAPMDAVAWSMGNDSGYRVTRIDYSDRQHPVTRVLSARLRPLTLEQMMVALRRDDQY
                     AAIAAQALYGKDFQMTREAPYGFAKRIKQAHDALNFRYSFALQAADFSPEVASAIALR
                     WVDKDVKKGVAYIYVITVCGSTKDYVVDSAATLLANNAATPIPAPDGLQVFGFDRKAE
                     LHWNRRQVGNFSAYDIERSDDGGKRWVAVSKLPYFSPDKMPPASGEIKEKSDTLIRKV
                     GALIRDHQVFVDSLPQDYHAYLYRVRGINAFAELSPWSEPVTVQGRDLTPPVAPMIDS
                     VRNTAGTLLRINWSQRVSSPDLAGYYISKGTSVKGPFYPLTSRLLPRDTRSFTDSAGI
                     PHLPNYYVIVAVDTAKNTAMSAAFPGYLTDTIPPAVPVGVAGSIDSS"
     gene            complement(1314..>2264)
                     /locus_tag="VKU83_06180"
     CDS             complement(1314..>2264)
                     /locus_tag="VKU83_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93174.1"
                     /translation="KAEDAERNIQLDAAAMPEEFDEFEINGIPSTCQDCDPQVFPAVY
                     PPMFSAAIPWNNAAQNDKFASDNLYANAFQMAFYNLKVDLGSPEVRDLMKPDNCLSTA
                     SMPFQPKLPPLSIQVGLVSKQVSVNGARLSAMKLSTKSGGSAAPAGGTASNSRMAAIP
                     VINLGVSVITASQPRLVWKHDNYIYADYSLLHQFAGNFLANQQNTMYVPFVGTLPVAL
                     EQALAYGNDVTLAAGEYGSITMDPASYTVRYNDPDLARIANALYGVSFQPLPPSSHTL
                     QLIYNYPFCSGCSLGIKVQESFNFGIVMPVPVRKTMTLTR"
BASE COUNT          514 a          687 c          584 g          479 t
ORIGIN      
        1 ggaagaatcg atcgagccgg ccacgccaac cggcacggcg ggcggtatcg tatcggtgag
       61 ataaccgggg aatgccgctg acatggcggt gtttttcgcc gtgtccaccg cgacgatgac
      121 ataataattg ggcaagtgcg gaatgccggc cgaatccgtg aaggaccgcg tatctctcgg
      181 caacaaacga cttgtcagcg gataaaacgg tcccttcacg ctcgtcccct tgctgatata
      241 gtaacccgca agatcaggac tgctgacacg ctggctccag ttgatcctca acaacgttcc
      301 agccgtattg cgcaccgaat cgatcatcgg cgcaaccggc ggcgtcaagt cccgtccctg
      361 aaccgtgacc ggctctgacc acggacttag ctcggcaaac gcattgatcc ccctcacgcg
      421 atacaaatag gcatgataat cttgcggcag gctgtctaca aaaacctggt gatcccgtat
      481 cagggcgccg accttccgga taagcgtatc gcttttctcc tttatttcgc cggaagccgg
      541 cggcatcttg tcaggactga aataaggcag cttgctcacc gcgacccaac gcttcccgcc
      601 atcatcgctg cgctcgatat catacgcact gaaatttcca acctggcgac ggttccaatg
      661 caactccgcc ttgcgatcaa acccgaatac ctgcaatccg tcgggcgcag ggataggcgt
      721 cgcggcgtta ttcgccaaga gggtcgcggc cgaatccacc acatagtcct tcgtcgaacc
      781 gcaaacagta atcacataga tataggcgac cccttttttg acgtccttgt cgacccagcg
      841 aagcgcaata gcactcgcca cttccggcga gaaatcggct gcctgcaacg cgaacgaata
      901 ccggaagttc aaggcgtcgt gcgcctgctt gatccgcttt gcgaaaccgt acggcgcttc
      961 ccgcgtcatc tgaaaatctt taccatacaa ggcctgggcg gcaatagcgg catattggtc
     1021 atcgcgccgg agcgcaacca tcatttgctc caaggtcaac ggccgtaacc ttgcggataa
     1081 cacgcgtgtc accggatgct gccggtcgct atagtcgatc cgcgtaaccc gataccctga
     1141 atcgttgccc atcgaccatg ccaccgcatc catcggcgcc cagcgcacaa cgatcgaatc
     1201 cgaatatgac tttgccagca tgcgcacatg caggtgccgc ggacctgtcg gctttttccc
     1261 ctggcccttc gccccaacgg ccatcaggat aatcaatatg atcagtagct ttttcatctc
     1321 gttaaagtca ttgtttttcg taccggcaca ggcatcacaa tcccgaaatt gaagctctcc
     1381 tgcaccttga tgcctaaact gcaaccacta cagaatggat aattgtagat caactgcaac
     1441 gtatggctcg aagggggcag cggctggaat gaaacgccat acagggcatt ggcaatcctg
     1501 gccagatctg gatcgttgta tcgaaccgta taggaagcgg gatccatggt gatgctgcca
     1561 tattctcccg cggcgagcgt aacgtcattc ccataggcca acgcctgctc cagcgccacc
     1621 ggaagcgttc cgacgaaagg cacatacatg gtgttttgct ggtttgccag gaaattcccc
     1681 gcgaactgat ggagtaacga atagtctgca tagatataat tgtcgtgctt ccatacgagt
     1741 ctgggctggc tcgccgttat cacagaaacg cccaggttaa tcaccgggat agccgccatc
     1801 cgcgaattcg acgcggtccc gccggcggga gcagctgacc cgccactctt cgtactcaac
     1861 ttcatagccg aaagcctcgc cccattcacc gacacctgct ttgacaccag ccccacctgg
     1921 atacttaacg gcggaagctt tggctgaaat ggcatcgatg ccgtggaaag acaattatcg
     1981 ggcttcatca ggtcgcgcac ctccggcgaa cccaggtcga cctttaaatt gtagaaggcc
     2041 atctggaacg cattggcgta caggttatcg ctggcaaact tgtcgttctg cgccgcattg
     2101 ttccagggga tcgccgcgct gaacatcggc gggtagacgg ccgggaatac ctgcggatcg
     2161 caatcctggc aagtggaggg aatgccgttg atctcaaatt cgtcgaattc ctcgggcatc
     2221 gccgctgcat ccagctggat gttgcgctcg gcatcttcgg cctt
//
LOCUS       DATGKE010000359         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1211109, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000359 DATGKE010000000
VERSION     DATGKE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211109"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VKU83_06185"
     CDS             <1..498
                     /locus_tag="VKU83_06185"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLI93175.1"
                     /translation="GFGFAPQQDGSFKKIPQIGNVQIEDFVEIGANVTIDRATIGSTI
                     IRKGAKLDNLIQIAHNVDIGSDTVIAAQAGISGSTKIGSGVMIGGQAGIVGHLQIADG
                     ARINAQSGVSKSIKAKGGAVTGSPAYEYTSALRSQAISRNLPELQKKLKELEAQLKQL
                     LAEKV"
     gene            complement(569..2305)
                     /locus_tag="VKU83_06190"
     CDS             complement(569..2305)
                     /locus_tag="VKU83_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HLI93176.1"
                     /translation="MASDNHIPSSDELRALLRQHWHFSEFRPQQEPIIRAVLSGRDTL
                     ALLPTGGGKSVCYQLPTLARPGLCLVISPLIALMKDQTDSLRRKNITAFFLHTGMSRR
                     ELITTLGTAANSNCKFLFVSPERLETELFASRLASLDIRLLAVDEAHCVSQWGYDFRP
                     AYLRIARLRAAIPGIPLLALTASATPQVAADITEKLEMMQPAVFRQPFTRPNLSYSVF
                     REDNKVRKITEVMNKVPGSGIVYCRTRRATREISEWLTRQGIPAAAYHAGLPREEREK
                     NQEDWIKDRVRIIVSTNAFGMGIDKPAVRTIIHADVPDSIENYYQESGRAGRDGAKAY
                     AVLLTDAGDTGRLEALPGIRFPAISSVRELYQHLMNYLQLPAGSGEGKYFDFDLGDFC
                     ERFQCQAQAVTDVLRLLEQEGLLSFQAQVALPSRVRFIVGKESLYEFEGSQPALRPLI
                     HALLRGYEGISDLPVFLRERQLAFTLRTDLRQIATGLAQLQAFGIIEYLPAKDSPQLY
                     FFRDRAAADELYIDPVKYKERKEVFASRVRAVARYLTLGDECRSRYLARYFGDKDAPA
                     CGICDNCLRLKR"
     gene            complement(2295..>2598)
                     /locus_tag="VKU83_06195"
     CDS             complement(2295..>2598)
                     /locus_tag="VKU83_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLI93177.1"
                     /translation="IGISLSDSSELARESADVVLMNSGLRQLPAALQLGRFTFATIRG
                     NLFWAFAYNVMAIPVAALGGLSPGIAALIMGLSDVVLAGNSLLLYTKKIAQPDHGE"
BASE COUNT          555 a          766 c          787 g          490 t
ORIGIN      
        1 ggcttcggct ttgcgcccca acaggacgga agctttaaaa agatcccgca gatcgggaat
       61 gttcagatcg aagactttgt ggagatcggc gccaacgtga ccatcgatcg cgcgacgatt
      121 ggctccacca tcatcaggaa aggggcaaag ctcgataatc tgatccagat tgcacataat
      181 gtggacatcg gcagtgacac cgtcatcgca gcgcaggctg ggatcagcgg cagcaccaag
      241 atcggaagcg gagtaatgat cggcggccag gcgggaattg tcggccacct gcagatcgct
      301 gacggcgccc gcatcaatgc gcaaagcggc gtatccaaat cgatcaaggc aaagggcggc
      361 gcggtgacag gcagcccggc ctatgaatat accagcgccc tccgcagtca ggcgatcagc
      421 cgcaacctcc ccgagctgca aaagaagctg aaggaattgg aggcgcagct aaagcaattg
      481 ctcgccgaaa aggtgtaagg accggcgcgc gacgccggcg tgcgcgcctt tgggccgctg
      541 gaaatcccag gtcaattcat tgccgctatc agcgttttaa acgcaggcaa ttatcgcaaa
      601 tgccgcaagc cggcgcatcc ttatcgccga aataacgcgc cagatagcgg ctgcggcatt
      661 cgtcgccgag tgtcaggtac cgggccacgg cccggacccg gctcgcgaag acttcttttc
      721 tttctttgta cttcacggga tcgatataaa gctcgtcggc ggccgcccgg tcacgaaaaa
      781 aataaagttg gggactgtcc tttgccggga ggtattcaat gatgccaaat gcctgcagct
      841 gagcgagccc cgtcgcaatc tgccgcaggt cggtcctcag ggtaaaggcc agctggcgtt
      901 cgcggagaaa aacgggcagg tcggatatgc cctcgtagcc gcgaagcagg gcgtggatca
      961 gcggccgcag cgccggctgg ctgccctcga attcgtaaag gctttccttt cccacgatga
     1021 acctgacgcg cgacggcagc gcgacctgcg cctgaaagct gagcagacct tcctgctcga
     1081 ggaggcgcag gacatcggta acggcctggg cttgacactg gaaccgctcg cagaaatcgc
     1141 ccaggtcgaa gtcaaaatac ttcccctcgc ccgagccggc cggaagctgc agatagttca
     1201 tgaggtgctg gtaaagttcg cggacagagc tgatagccgg gaaacggatg ccgggcaggg
     1261 cttcgagcct gccggtatcg cccgcatccg taagcaggac ggcgtaagcc tttgcgccgt
     1321 cgcggccggc gcggcccgat tcctggtagt agttttcgat cgaatcgggg acgtccgcgt
     1381 ggatgatcgt ccgtaccgcc ggcttgtcga ttcccatgcc gaaagcattc gtgctaacga
     1441 tgatcctcac ccgatccttt atccagtcct cctggttctt ttctctttct tcccgcggga
     1501 gtcccgcatg atatgccgcg gcggggatgc cttgccgcgt gagccattcg ctgatctcgc
     1561 gggtggcacg gcgggtccgg caatagacga taccgctgcc ggggaccttg ttcatgacct
     1621 ccgttatctt ccggacctta ttgtcctcgc ggaacacgct gtaggaaagg ttcggccgcg
     1681 taaatggctg ccggaacacc gccggctgca tcatttcgag cttttccgtt atatcggcgg
     1741 ccacctgcgg cgtcgcggaa gccgtcagtg caagtaacgg gatgccggga atcgccgccc
     1801 gtagcctggc gatgcgaagg taagcgggcc tgaaatcgta tccccattgc gacacgcaat
     1861 gggcctcgtc gacggcgagc agccggatat ccagcgaagc cagccggctt gcgaacagtt
     1921 ccgtttcgag cctttcgggc gagacaaaaa gaaatttgca attgctgttg gccgcggtcc
     1981 cgagcgtggt gatcagctcg cggcggctca tcccggtatg caaaaaaaag gcagtgatat
     2041 tcttccgccg caggctgtcc gtctggtcct tcattaaggc aatcagcggg ctgatgacca
     2101 ggcacagacc cggcctggcc aacgtcggca gctgataaca gaccgatttg ccgccgccgg
     2161 tgggcagtaa agccaaggtg tcgcggccgc taagtacggc acggatgatc ggttcctgct
     2221 gtggccggaa ttcggaaaaa tgccagtgct gccgaagcag cgcccgcagt tcgtcactgg
     2281 aaggaatatg gttatcactc gccatgatcc ggttgggcaa ttttctttgt atacagcaat
     2341 agcgagtttc ctgccaatac gacgtcgctt agccccatga tcagcgcggc gatgccgggg
     2401 ctcaaaccac ccagcgcagc cacgggaatg gccataacat tgtatgcgaa tgcccaaaac
     2461 aggttcccgc gaatggtcgc aaacgtaaac cggcccagct gcagtgcggc aggaagctgg
     2521 cgaagcccgc tgttcatcag cacgacgtcc gccgattccc ttgccagctc ggatgagtcg
     2581 ctcagggaaa tgccgatc
//
LOCUS       DATGKE010000360         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_429031, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000360 DATGKE010000000
VERSION     DATGKE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429031"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VKU83_06200"
     CDS             <1..221
                     /locus_tag="VKU83_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLI93178.1"
                     /translation="FRPVAAGVAALKFFRIYNRSGELMYSTSQLGQGWDGTSSGKPQV
                     AGTYVWMLEGVTYAGKLIQKRGTVILIR"
     gene            complement(232..1233)
                     /locus_tag="VKU83_06205"
     CDS             complement(232..1233)
                     /locus_tag="VKU83_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLI93179.1"
                     /translation="MTTTPITAPATPITAPAINEHKEIPGNPSTAKSSRLRLNDRSNG
                     EPLRVLSEADWDFWVTNGYVVIRGAVPKEQVKRLADYLWEYEGKDPNDPTSWYRKPEV
                     DMQMKELVNTGMVEIYNHQYMWDNRQYPKVHQAFADIWGTEKLWVTIDRANLNFPLAP
                     GFEYKGFIHWDYDPETKPQNVQGVLALADQMDENMGGFQCIPELYRTYETWKLTQPEN
                     RDHYKPDTTGFEIVKVPMEAGDLLIFNSLEPHGIRPNRSGNKVRIAQYISMMPAQEEN
                     EELRQWRINSWQSRNPMEGYAFPGDPRQREKRNPVAVLSPLGEKLLGLKRWSEGLGE"
     gene            1339..2184
                     /locus_tag="VKU83_06210"
     CDS             1339..2184
                     /locus_tag="VKU83_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI93180.1"
                     /translation="MKTVELEKIEFPPEKSYKVFSPRLRNRFYWHYHPEIELVYVEAD
                     AGIRHVGRHISTYTRSDLVLIGGNLPHLNFDYRLRNDYHQIVVQLRTDFLGKAIDIAP
                     ELNDIAKLFHNARHGIAFFGETKEEVAQMLYRMDGLPAIDQLLLLINIFETLAITSEG
                     QELNPGDQQPESSILKDNIRMGEVYKYIDAHYDKEPDVNVIAKKVHLTTAAFCRYFKK
                     QTNITFTAFVNQYRIERAKNLLMQGRNVSETCWAVGLDSLSYFNKLFKSLVGQNPSEF
                     KGRYT"
BASE COUNT          499 a          633 c          580 g          548 t
ORIGIN      
        1 ttttcaggcc ggtcgcggct ggtgttgccg ccctcaaatt cttccggatc tataatcggt
       61 ctggcgaact gatgtacagt acctcacaac ttggccaagg ctgggatgga acttcatcgg
      121 gtaagcccca ggtggccggc acctatgtct ggatgctgga aggtgttact tacgccggca
      181 aactgatcca aaaaaggggg acggtcatcc tcatccgtta aagtgccagg cctactcccc
      241 gagcccctcc gaccatcgct tcagtcccag caacttttca ccaagaggcg acaggacggc
      301 gacgggattc cttttttccc gctggcgcgg gtctcccgga aaagcgtaac cctccatcgg
      361 gttgcggctt tgccagctgt taatgcgcca ttgccgaagt tcctcgttct cttcctgggc
      421 aggcatcatc gaaatatatt gcgcaatcct gactttattg ccgctgcggt tcggccggat
      481 accatggggt tcgaggctgt tgaaaatgag cagatcaccg gcctccatcg gcaccttcac
      541 gatctcaaag cctgtcgtat ccggtttata atgatcccgg ttttccggct gcgtcaattt
      601 ccacgtctca tacgtccggt aaagctcggg gatgcactgg aagccaccca tattctcatc
      661 catctggtca gcgagggcca gcaccccctg gacattctgc ggcttggttt caggatcata
      721 atcccagtga atgaacccct tgtattcgaa accgggggcc agcggaaaat tcaggttggc
      781 cctgtcgatc gtgacccata gcttttccgt tccccagata tcggcaaagg cctggtgtac
      841 cttgggatac tgccggttgt cccacatgta ttggtggttg tagatctcga ccatgccagt
      901 gttcaccagt tccttcattt gcatatcgac ctcgggctta cggtaccatg acgtcggatc
      961 attcgggtct tttccctcgt attcccaaag gtagtccgca aggcgcttca cctgttcctt
     1021 cgggaccgcg cctctgatga ccacatagcc gttagtgacc cagaaatccc agtcggcctc
     1081 gctgagtaca cgcagcggtt ccccattgct gcggtcattc aggcgaagcc gcgaagattt
     1141 ggcagtggag ggattacccg gaatttcctt atgctcattg atggctggcg ccgtgatggg
     1201 ggtggctggc gccgtgatgg gggtggttgt catgatgcaa aattttatgg tccaaagtta
     1261 ctgcctcgtt gtcccggtgg cgtcctgcct tttgaccact atttgctctc tattgaccat
     1321 tttactagat ttgcatccat gaaaactgtc gaattagaga aaatcgagtt tccgcccgaa
     1381 aaatcctaca aggtcttctc cccccggctg cggaacaggt tctattggca ttaccatccg
     1441 gagatcgagt tggtgtatgt agaagccgac gccggcatcc gccatgtggg ccgccatatc
     1501 tccacgtata ccaggtcgga tctggtgctc atcggcggca acctgccgca tctgaacttt
     1561 gactaccggc tgagaaacga ctatcatcag atcgtcgtcc agctgcggac ggactttttg
     1621 ggcaaggcaa tcgacattgc tcccgaactg aatgatattg caaaactctt ccataacgcg
     1681 cgccatggca ttgccttctt cggggaaaca aaggaagagg tggcgcaaat gctctaccgc
     1741 atggatggtt tgccggccat cgatcaactg ctgctgctga tcaatatctt cgagacgctg
     1801 gcgattacca gtgaggggca ggaactgaac cccggggacc agcaacccga aagctccatt
     1861 ttaaaggaca atatcaggat gggagaagtc tacaaatata tcgatgcaca ttatgacaag
     1921 gagccggacg tgaacgtcat cgcgaaaaaa gtccacctga ctaccgctgc gttttgccgg
     1981 tacttcaaaa aacaaacgaa tatcaccttc accgcattcg tcaaccaata ccggatcgag
     2041 cgcgcgaaga acctgctcat gcaaggccgc aacgttagcg agacctgttg ggcggtcggt
     2101 ctggacagcc tttcctactt caataaattg ttcaagagcc tcgttgggca gaacccgtcg
     2161 gaattcaaag gccgctatac ttaaccggcc attctataga ttctgacgct tcatttcatc
     2221 caccgcgtgg ttggccgcac gcgcagtaag cgccatgaaa
//
LOCUS       DATGKE010000361         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_834331, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000361 DATGKE010000000
VERSION     DATGKE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834331"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VKU83_06215"
     CDS             complement(<1..1469)
                     /locus_tag="VKU83_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI93181.1"
                     /translation="MMRKMAKASKPVLSLFFALSLTVSSLAQRSDAGKEVIYHIFQRS
                     FFDADGDLKGDLRGIIDKLGYLRELGVTSVLLTPLYQSVFYHNYFADDWKTIDPDYGS
                     IQDFADLARALHARGMKLYIDMETQYVTDHHEWWKSSVGNLRSPDRNYLLFDDSEHQK
                     PSPIVWGITELKGYNGASRKVATVNLRDPGVLAFNLRLFSYFADPNGDGRFDDGVDGF
                     RLDHTMDDLDGNPRLTDLFARFWAPLIAGLKRLNPRLSFVAEQANWGSFGFDYFSKAT
                     IDRVFAFRLAFAIQSFDKAGLAKAADTTLRQLPNGKEQVVFLENHDLDRFASRVKMDP
                     GKERIGATLNLLLGGIPAIYYGQELGMFGAGGNNRYGMTDANDIPRREAFEWYAADTG
                     KGMALWYKGTGPWWDSTNLRPFDGISYQEERSDAKSLWNWYRQLLALRRAHAVLQTGR
                     YEPLANSSPTVFSWRRVLGKSVAYLAVNLSAQSAQAEIG"
     gene            1535..2443
                     /locus_tag="VKU83_06220"
     CDS             1535..2443
                     /locus_tag="VKU83_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI93182.1"
                     /translation="MDKKTVFSGFSNKPCAMKALFEDIDSKRGADSYLAYRYETALFP
                     FQWHYHPEYELTLILEGSGERMVGDNHEYFAPGDLVLLGPGLPHTWVSKCRSAAVVIQ
                     FAESFIAPLLQYPECAAIRQLLAKAQQGLYFPLKDVEGQLAAVGQLPARTAVSRITGL
                     LDVLQVLAGPDVAPVPLASAYFQPAQGRKTEGRIGKVFQYVHRYSGEAVSLKEVAALI
                     NLSESAFCKFFKRTTGRTFSAYLTDIRIGHACHLLSESDETIAEIAYRSGFDSLTYFN
                     RMFLRNKGVRPREFRKAIAGSAVIPS"
     gene            complement(2430..>3212)
                     /locus_tag="VKU83_06225"
     CDS             complement(2430..>3212)
                     /locus_tag="VKU83_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93183.1"
                     /translation="WGLELVAGRNLPAIPAEKEDRYVLINQKMVSELKYPSAQRAVGQ
                     HLLLEGRDVEIIGVVKDFQFTDLTMKLSPIMLRNRRPEFGYITVRVRGKDLAGAVSFM
                     QDSWRRVNPGSKFEYEFFDEQVATLHAMMSDTAGVLGLLAFLAVTISCLGLLGMAAYT
                     AETRRKEISIRKVLGSNAWQVLMMLSKGFLTLLGIAIVISVPAAILINNLWLQEFASR
                     ISISAGLILLNVAILLTLSFLIIVSQTWRVSTASPVTGLRME"
BASE COUNT          699 a          912 c          930 g          671 t
ORIGIN      
        1 atcccgattt ctgcctgcgc ggactgggcc gacaggttga ccgcaaggta tgccacgctt
       61 tttcccagca cccgccgcca ggaaaaaacg gtgggactcg aattggcaag gggttcgtag
      121 cggccggttt gcaataccgc gtgcgccctg cggagggcga gaagctgccg gtaccaattc
      181 cagagcgact tcgcgtcgga gcgttcctcc tggtaggaaa tgccgtcgaa gggccggagg
      241 ttggtgctgt cccaccatgg tccggtgccc ttataccaga gggccatgcc tttcccggtg
      301 tcggctgcat accattcgaa ggcctcgcgg cgggggatat cgttggcgtc ggtcatccca
      361 taccggttgt tgccgccggc cccgaacatg ccgagctcct gcccgtaata aatggcgggg
      421 atgccgccca gcaggaggtt gagggtcgcg ccgatccgtt ccttgcctgg atccattttt
      481 accctgctgg caaagcggtc caaatcatgg ttctccaaaa aaacaacctg ttccttcccg
      541 ttaggtaatt ggcgaagggt ggtatccgcc gctttcgcga gcccggcctt gtcgaacgat
      601 tggatggcga aggccaggcg gaaggcaaag acccggtcga tggtggcctt gctgaaataa
      661 tcaaaaccga acgaacccca attggcttgc tcggctacaa agctaaggcg cgggttcagc
      721 cgcttgagcc cggcaatcag cggcgcccag aaccgggcga acagatccgt aagccgcggg
      781 tttccatcga ggtcgtccat ggtatgatcg agccggaagc cgtcgacccc atcgtcgaac
      841 ctgccgtcgc cgttcgggtc cgcaaaatag gagaacaacc gcaggttaaa agcgagcacg
      901 cccgggtcgc gcaggttgac cgtcgccact tttcgggacg cgccgttgta gcctttcaac
      961 tcggtgatac cccagacaat ggggcttggt ttctggtgct ccgagtcatc aaacagcaga
     1021 taatttctat ccggcgaccg gaggttccca accgaagact tccaccattc atgatggtcg
     1081 gtaacatatt gggtctccat gtcgatatag agcttcatgc cgcgcgcgtg cagcgcccgg
     1141 gctaaatcgg cgaagtcctg gatcgagcca tagtccggat cgatcgtctt ccagtcatcc
     1201 gcaaaatagt tgtgataaaa aaccgattgg tacaggggtg taaggaggac cgaggtgacg
     1261 cccagttccc gcaggtaacc gagtttatcg ataatgccgc ggagatcgcc tttgagatcg
     1321 ccgtcggcgt cgaaaaaact ccgctggaag atatggtaga tcacctcctt tccggcatcg
     1381 ctgcgctgtg ccagcgacga gacggtcagg gaaagcgcga agaaaagact taggacaggt
     1441 ttggatgctt tggccatctt cctcatcatg ggatgcaaac ctaaggaagc agcaaggcaa
     1501 atttccgcac ttaacagcca ttaaatggca caatatggac aaaaaaaccg tattttccgg
     1561 ttttagcaac aaaccctgtg cgatgaaggc gcttttcgaa gatatcgaca gcaaacgcgg
     1621 cgcggactcc tacctggcct acaggtatga gacggcgcta ttcccctttc aatggcatta
     1681 ccatccggag tatgaattga ccttaatcct cgaaggttca ggagaaagaa tggtcggcga
     1741 caatcatgag tattttgctc cgggcgatct cgtgttactg ggaccggggc tgccgcatac
     1801 ctgggtgagc aaatgcaggt cggccgccgt ggtgatccag ttcgcggaaa gcttcatcgc
     1861 ccccctgctg caatatccgg aatgcgccgc catccggcag ttgctggcaa aggcccagca
     1921 ggggctgtat tttccgttga aggatgtgga agggcagttg gcggcggtcg gacaattgcc
     1981 ggcgagaacc gcggtcagcc ggatcacggg cctcctggac gtcttgcagg ttcttgccgg
     2041 tccggatgtc gcgccggtgc ccctggcctc ggcttatttt cagcctgcgc agggcaggaa
     2101 gaccgaaggc cgcatcggaa aggtctttca gtatgttcat cgttacagcg gggaagcggt
     2161 ctcgctgaag gaggtcgcgg cgctgatcaa tttatcggag agcgcttttt gcaagttttt
     2221 caagcggaca acgggccgca ccttttccgc ctatctcacc gatatccgga tcgggcatgc
     2281 ctgtcacctg ctcagcgagt cggacgaaac gatcgctgag atcgcctacc ggtcagggtt
     2341 cgacagcctg acctatttca accggatgtt cctccggaac aagggcgtga ggccgcggga
     2401 atttcgcaaa gccattgccg gttcggcggt tattccatcc tgaggccggt tacagggctc
     2461 gcagtcgata ccctccaggt ctgcgagaca atgatcagga aacttagcgt cagaaggatc
     2521 gccacattta ataagatcaa cccggcgctg atactgatcc gcgacgcgaa ttcctgcagc
     2581 cacaggttgt tgatcaggat cgctgcgggc accgagatga caatggcgat gccaagcagc
     2641 gtcaaaaagc cttttgacag catcatgaga acctgccagg cgtttgaacc gaggactttc
     2701 cggatgctga tctcctttct cctggtctcc gccgtgtagg ccgccatccc caaaagcccg
     2761 aggcaggaga tcgttacggc gaggaaagcc agcagaccaa gaacgccggc ggtatcgctc
     2821 atcatggcat gaagggtcgc cacctgttca tcgaaaaatt cgtattcgaa tttcgagccc
     2881 gggttaaccc ttctccagct gtcctgcatg aatgaaaccg cgccggcgag gtccttgccg
     2941 cgaacgcgca ccgtgatgta gccgaattcg ggtcggcggt tccgaagcat gatgggcgac
     3001 aatttcatgg tcaggtccgt gaactgaaag tctttgacga ccccaatgat ctccacgtcc
     3061 cggccctcaa gcaacaggtg ttgcccgacc gcccgttgcg ccgaaggata tttgagctca
     3121 ctgaccatct tctggttgat cagcacatag cgatcctcct tttcagcagg gatcgcggga
     3181 aggttcctgc ctgccaccaa ttccaatccc ca
//
LOCUS       DATGKE010000362         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_709675, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000362 DATGKE010000000
VERSION     DATGKE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709675"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VKU83_06230"
     CDS             complement(<1..285)
                     /locus_tag="VKU83_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93184.1"
                     /translation="MRWIFFAIGVMGATVAYSQQNYNGVDAHLGNLYMLSNAQSRSIS
                     PENFHGAKGEGGKATTGTGAGPARDLGQGWKISPSVVIKAKSTFTVAEIDG"
     gene            427..759
                     /locus_tag="VKU83_06235"
     CDS             427..759
                     /locus_tag="VKU83_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI93185.1"
                     /translation="MFRSDAQLIREIREYIDAHLYEENHIRDLCLRFSINREKLQLGF
                     HRLVGRTVHSYIIRRRIDIAAHRLRKTDDSIKVIALDSGYKKQRSFAKAFKSVYNLSP
                     ADYRKLHR"
     gene            888..>2063
                     /locus_tag="VKU83_06240"
     CDS             888..>2063
                     /locus_tag="VKU83_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93186.1"
                     /translation="MEQIHPKQFPSDSGFSKYVLRDNRTFLLIITGVMWLEFVIFKIL
                     YPFPDFFSDSYSYITAAWLRMDVNIWPIGYSRFLAGFHHWVSHSGSALITFQYLAWCC
                     SALFFYLTLTYFYPTGKNTRIFLNLFLFFNPLIFYTANYVTSDILFISMSTTWLSLLL
                     WILNRPRITHVLAQAVLTFLTFTFRYNAMIYPLIAAGVILLSSLRPWIKALGMILGPA
                     LIIPFILWSARAARLVTGVPQFPPILGGWQWANNALYFRGFIRVDTNAFPSTQTAELD
                     GMARRFFSLPDRPQHLLADEVANFFIRHPEAPLKQYMARHFKPRNAFEDVAAWGKSAI
                     VFDQYGKFLIRKHPLAYARYYLLVNTKNYLIPPLEKLEIYNLGQDRMWPQAQGWFEYP
                     "
BASE COUNT          436 a          642 c          485 g          500 t
ORIGIN      
        1 cccgtcgatc tccgcaaccg tgaacgtgga ctttgccttg atgaccacgg aggggctgat
       61 cttccagccc tggcccaaat cccgcgccgg gccggcgccc gtgcccgtcg tggctttccc
      121 gccttcgcct ttagcgccgt ggaagttctc cgggctgatg gagcggcttt gagcgttgga
      181 cagcatgtac aaatttccaa gatgcgcatc cacgccatta taattttgct gcgaataggc
      241 caccgtggcg cccatgactc cgatagcgaa gaaaatccat ctcataaaca accgtttagc
      301 cgattaaggt aaggatattt ctataaatca ttgcccgccg gccaccttcg gtaatggccg
      361 cgctgaaagg cggccgcagc cgttttatat attttgcatc cgaatcccat taaacctgtc
      421 cttatcatgt ttcgaagtga cgcccagttg atccgggaga tcagggaata tatcgacgcc
      481 catctttacg aggaaaacca tatcagggac ctatgcctgc ggttcagcat caaccgcgag
      541 aagctgcagc tcggattcca ccgtctcgtc ggtcggaccg tccattccta catcatccgc
      601 cggcgtatcg atatcgccgc ccatcgcctg cgcaagacgg atgactccat aaaagtcatt
      661 gccctcgaca gcggctataa gaaacagcgc agcttcgcga aagcattcaa atcggtctac
      721 aatctaagcc cggcggacta ccggaaactt cacagataac agcttcccca tccaactatg
      781 ggctgttgca tccggcccga ccattccgta acactatcgt cccctttcgg aataacaatc
      841 cgtctacctt cccggtattt tccggcgccg ccataaatca tgcacgcatg gagcaaatac
      901 acccaaaaca atttccgtcc gactcgggat ttagcaaata cgttttaagg gacaacagga
      961 ccttcctgct gattatcacc ggcgtcatgt ggttggaatt tgtgatattc aaaatcctct
     1021 acccctttcc agattttttt tccgacagct acagctatat cacggccgca tggctacgca
     1081 tggatgtcaa tatctggccg atcgggtatt cgcggttcct ggcagggttt caccattggg
     1141 tgagccactc cggctcggcg ctgataacgt tccagtatct cgcctggtgc tgttccgcgc
     1201 tgttctttta tcttacactg acctactttt atccgacggg caagaacacg cgcattttcc
     1261 tcaacctgtt cctgtttttc aatccgctga tcttctacac cgccaattat gtgaccagcg
     1321 atattctctt tatcagcatg agcacaacct ggcttagcct gctcctctgg atcctcaacc
     1381 ggccccggat aactcatgtc ctcgcccagg ccgtgctgac gttcctcact ttcactttcc
     1441 gttacaatgc gatgatctat cccctgatcg ccgcgggggt catcctcctg tccagtctgc
     1501 ggccctggat aaaagcgttg ggcatgatcc tgggccccgc gctgatcatc ccttttattc
     1561 tctggtccgc ccgggccgcc aggctggtga ccggtgtccc gcaattccca cccatcctcg
     1621 ggggctggca gtgggccaat aatgctttgt acttccgcgg tttcatccgg gtggatacca
     1681 acgccttccc ttcgacgcag acagcggagc tggacggcat ggcgcggcgc ttctttagcc
     1741 tgcccgaccg gccgcagcat ctcctggcgg acgaagtcgc caatttcttc atccgtcatc
     1801 cggaggcgcc cctgaaacaa tatatggcgc ggcacttcaa gccccgcaac gcattcgagg
     1861 acgtcgccgc ctggggaaaa tccgcgatcg tttttgatca gtatgggaaa tttctgatca
     1921 ggaagcatcc gctcgcctac gcccggtact acctcctcgt caacacaaaa aattatttga
     1981 ttccgcccct cgaaaagtta gagatctaca atctcggcca ggaccgtatg tggccgcagg
     2041 cgcaaggctg gttcgaatat ccc
//
LOCUS       DATGKE010000363         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1059796, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000363 DATGKE010000000
VERSION     DATGKE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059796"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VKU83_06245"
     CDS             complement(<1..922)
                     /locus_tag="VKU83_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93187.1"
                     /translation="MAQHYSQLRAMLAITKGALIAIFRSPAAVAFSFGFPLVFILVFG
                     FINGGAPTVTFALTNQRDTAAPVIRGLLKNPMIKLSAEKDTTADRMNLQKGRIAAIIS
                     LDSSKSPAGFTQYTVRLQTSSASSDKYPVLKLGLAQVFEQSVERYAAKEYRPIIVDEL
                     PVLPGRQYSMIDFILPGMLGFSLLSAAVFGVAFLFFSLRQQLVLKRFYSTPISKAYIV
                     LGEGLARVLFQLITAIVILVLGWTAFHFTMVNGWITFWEMLFLSLFGLIVFMGCGFII
                     SSVAKNESVIPIFANVITLPQFLLSGTFFTI"
     gene            complement(938..2695)
                     /gene="sppA"
                     /locus_tag="VKU83_06250"
     CDS             complement(938..2695)
                     /gene="sppA"
                     /locus_tag="VKU83_06250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLI93188.1"
                     /translation="MQGFLKSFFAALLAVIVFAAFCVLVLTIIIGGVLSPSRPTVGGN
                     AVLVVDLGQDYREQSLGNPLAALGVDDEYDVPGVYDMVRLIRHAAQDSAIHGIFLKCN
                     SDPNGFATNEEIRTALAEFRKSGKFIYAYGDVVPQKAYYVASVADKIYCNPKGGVVWR
                     GFASQMLFLKDALDRLQIQPQIFYAGKFKSATEPLRATRMTDANRLQLTVLLGDLYNQ
                     FLATTAKARGLDTNDLRKCVNEDLIRTAQDALDHKLVDGLKYDDEVKDDLRDRLHLGH
                     HSYLNFVAAGKYAKAVNYKRTGKDQISVIYAEGEVVEGKGEKDEIGSDTYRWLIRKAR
                     FDNDVKAIVIRVNSGGGSSLASENIWREVALARKEGKPVVISFGDVAASGAYYLSCDA
                     DSIFAMPGTITGSIGVFTIIPNVEGFFKNKLGVTFDVVKTAPEADGMTITRPLTPAEQ
                     RLIQNDVDTIYRDFKSRVAAGRKKTMDYIDSIGQGRIWSGQMGASLGLVDRIGGMQDA
                     IDCAARMARTSDYRLVEYPEPRGFLERLLGGYRRSLSATSMKEELGPDGYRTYMTIKR
                     VKAMVGASQTRMPFDMDIE"
     gene            2789..>3042
                     /gene="folK"
                     /locus_tag="VKU83_06255"
     CDS             2789..>3042
                     /gene="folK"
                     /locus_tag="VKU83_06255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLI93189.1"
                     /translation="MNISYLLIGGNQGLREGQLATARAHIADSAGVVRRASSLYETAP
                     WGKTDQGWFLNQALQVETPVDAAALLMILLGIEEKMGRKRS"
BASE COUNT          684 a          916 c          779 g          663 t
ORIGIN      
        1 cgatcgtaaa gaaagttccg ctcagcagga actgcggcag tgtgatcacg ttcgcaaaga
       61 tgggaattac gctttcattt ttagcgacgc tgctgatgat gaaaccgcag cccatgaata
      121 cgatcaggcc aaagaggctc aggaacagca tttcccaaaa agtgatccag ccgttcacca
      181 tggtaaaatg aaatgcggtc catccgagca ccaggatcac gattgccgtg atgagttgga
      241 acagcaccct ggccagcccc tccccaagca cgatataggc cttgctgatc ggcgtcgagt
      301 aaaaccgctt caggaccagc tgctggcgaa ggctaaaaaa gaggaatgcc actccgaaca
      361 ccgctgcgct gagcaatgag aaaccgagca ttcccggcag gatgaaatcg atcatggaat
      421 actggcggcc cggcagcaca ggaagctcat cgacgatgat cggccggtat tccttcgccg
      481 cgtaccgctc cacagactgt tcgaaaacct gcgccagccc aagcttcagc acggggtatt
      541 tgtccgagga cgcgctcgac gtttgcagcc ggactgtgta ctgggtgaac ccggccgggg
      601 atttcgagga atccagggaa atgatcgcgg caatccgccc cttctgaaga ttcatgcggt
      661 ccgccgtagt gtccttttcg gcgctcaatt tgatcatcgg attcttcaac aagccgcgga
      721 taacgggcgc cgccgtgtcc cgctgattcg tcagggcaaa tgtcaccgtc ggagctccac
      781 cgttgatgaa gccaaaaacc aggatgaaca cgagcgggaa tccaaaactg aaagccacgg
      841 ccgccgggct ccggaaaata gcgatcagcg caccttttgt gatcgccagc atcgcccgca
      901 gctgggaata atgttgtgcc attttcgttc caatacttta ttcaatatcc atgtcaaaag
      961 gcatccgcgt ttggctggcg cctaccatcg ccttcactct tttaatcgtc atataagtcc
     1021 ggtaaccatc ggggcccagt tcttccttca ttgacgtcgc gcttagcgac cgccggtagc
     1081 ctcccagcag ccgttccagg aagcccctcg gctcagggta ttcgaccagc cggtaatccg
     1141 acgtccgggc cattcgtgca gcgcaatcga tcgcatcctg catcccgcca atcctgtcca
     1201 caaggcccag ggatgcgccc atctgcccgc tccagatccg gccctggcca atactgtcga
     1261 tgtagtccat cgttttcttt cgtccggcag cgacccgcga cttgaaatcc cggtagatcg
     1321 tgtccacatc attctggata agcctttgct ccgccggggt caggggccgc gttatcgtca
     1381 tcccatcggc ctccggcgcc gtctttacca cgtcaaacgt cacgcccagc ttattcttaa
     1441 agaaaccttc aacgttggga atgatcgtaa atacgccgat cgacccggtg atcgttcccg
     1501 gcatggcaaa gatactgtcc gcgtcgcagc tcaggtaata ggcgccgctg gcggcgacgt
     1561 ccccgaaact gatcacgact ggctttcctt ctttccgcgc aagcgccacc tcccgccata
     1621 tgttctcact tgccagcgaa ctgccgccgc ccgaattgac ccggatcacg atggccttga
     1681 catcgttgtc aaacctcgct ttccttatca gccaccggta ggtgtcggaa ccgatctcat
     1741 ccttttcgcc tttcccttcg accacctccc cttccgcata gataacgctg atctggtcct
     1801 tccccgttcg tttgtaattc acggccttgg catatttgcc ggccgcgacg aagttcagat
     1861 aggaatgatg gccgaggtgc agccggtcac gcaggtcatc tttcacttcg tcgtcgtatt
     1921 tcagcccgtc caccagtttg tgatccagcg catcctgggc cgtccggatc aggtcttcat
     1981 tcacacattt cctgagatcg ttcgtatcga ggccgcgcgc ctttgccgtg gtcgccagaa
     2041 actgattgta gagatcgccc agcaggacgg ttagctgcag ccggttggca tcggtcatcc
     2101 tggtggccct gagtggctcc gtagcacttt tgaatttgcc ggcataaaag atctgcggct
     2161 ggatctgaag ccggtcgaga gcatccttca gaaaaagcat ctgcgacgca aatccccgcc
     2221 atacgacgcc gcctttcggg ttacaataaa ttttgtccgc cacgctggcg acatagtagg
     2281 ctttttgcgg aacaacgtcc ccgtaggcat agatgaactt gccgcttttc ctgaactcag
     2341 ccagcgctgt ccggatttcc tcattcgtcg cgaacccgtt gggatcgctg ttgcacttca
     2401 gaaatatccc atggatagcg gaatcctgcg cagcatgccg gatcaaccgg accatatcat
     2461 atactcccgg aacatcatat tcgtcgtcca cgcccagcgc ggcaagcggg ttgcccagcg
     2521 actgttccct gtagtcctgc cccaggtcga cgacgagcac cgcgttaccg ccgacagtcg
     2581 gccgggaagg cgataatacg ccaccgatga tgatcgtgag caccagcacg cagaatgccg
     2641 caaaaacgat caccgccagc agtgctgcaa agaacgattt taaaaagcct tgcatgaaat
     2701 gaaaattata tttatcgacg tgagaaagtt atctcttcct tccacgccaa aaataaagat
     2761 atttgggccc ccgaaagtaa tggaatctat gaacatctcc tatttattga taggtggcaa
     2821 ccagggtctc cgggaaggcc aattggccac ggccagggcc catattgccg actccgccgg
     2881 cgttgtacgg cgggcgtcgt cgctatacga aacggctcca tggggaaaga ccgatcaggg
     2941 atggtttctc aatcaggccc tgcaggtgga gacaccggtc gatgccgctg cattgctgat
     3001 gatcctgctc ggcatcgaag aaaaaatggg acgcaaaaga tc
//
LOCUS       DATGKE010000364         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_749814, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000364 DATGKE010000000
VERSION     DATGKE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749814"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VKU83_06260"
     CDS             complement(<1..253)
                     /locus_tag="VKU83_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93190.1"
                     /translation="MTNIESLVFDLGGVLIDWNPEYLYHKLIPDEKERKWFLDEICSP
                     EWNEEQDAGRSLAEGTEFLVRKYPQHEAAIRAYYGRWQEM"
     gene            complement(250..1401)
                     /locus_tag="VKU83_06265"
     CDS             complement(250..1401)
                     /locus_tag="VKU83_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HLI93191.1"
                     /translation="MKRMIESKLPQVGTTIFTVMSSLAAELGAVNLGQGFPDFPMSGE
                     LISLVAQAMREGHNQYVHMNGLASLREVLAAKIAGLYGAAIDPETQITVTPGGTYAIY
                     TALTTVLRQGDEVIVFEPAYDSYIPSIELNGATPVRIGLRFPDYRIDWEEVRRRVTAR
                     TKMILLNSPHNPTGAVLLPEDIEALRAIVRDTGILIHSDEVYEHLIFDRIPHQSMLRY
                     PDLLERSFVSFSFGKIYHCTGWKLGYSVSSPALMKEFRKVHQFNCFSCHAPSQFGLAK
                     YLANKDSYLSLGAFMQKKRDYFAQLMEQTRFTSLPSHGSYFQLYRYGRISDEPDTAFA
                     IRMTKEYGVAMIPVSVFYTSGKDDRVVRFCFAKKESTLEAAVERLIKIV"
     gene            complement(1398..2117)
                     /locus_tag="VKU83_06270"
     CDS             complement(1398..2117)
                     /locus_tag="VKU83_06270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLI93192.1"
                     /translation="MSAFPIHNILFLDIETVSQYSSFHEVPEDWQELWRHKADSLVRD
                     KAPVTPEELYGRAAIYAEFGKVICVSCGVLSGPPAGRKLLLKSFYGDDEKDLLMRFYD
                     LLHKWGSEGQKYLCAHNGKDFDFPYLCRRMVIHGMNLPSVLNLAGKKPWEVPHLDTME
                     LWKFGEYKCYISLHLLANTLGIPTPKDDIDGSQVGDVYWVQRDLPRIVTYCQKDVVTV
                     AQVYLRLHGEPPVRQENIEIR"
     gene            complement(2146..>2375)
                     /locus_tag="VKU83_06275"
     CDS             complement(2146..>2375)
                     /locus_tag="VKU83_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93193.1"
                     /translation="KDYLENAVAGDNVIVIDATRCMVHAPNEKLLLLQGLDNFIVVDT
                     SDVLMICKREKEQEIKEYVAEVRRNKGDQYL"
BASE COUNT          501 a          695 c          648 g          531 t
ORIGIN      
        1 gcatctcctg ccaccgcccg tagtaagcgc ggatcgccgc ctcatgctgc gggtatttcc
       61 tgacgaggaa ttctgttcct tcggccaggc tgcgcccggc atcctgttcc tcgttccact
      121 ccggcgaaca gatctcgtcc aggaaccact tccgttcctt ttcatcggga atgagcttat
      181 ggtacaggta ctccgggttc cagtcgatca gaactccgcc cagatcaaaa acgagtgatt
      241 cgatattcgt catacaattt ttattaagcg ttccaccgcc gcctccaggg tcgattcttt
      301 ttttgcgaag cagaaccgga cgacccggtc gtctttgccc gaagtataaa aaacggatac
      361 cgggatcatg gccacgccgt attccttcgt catgcggatc gcaaacgccg tgtccggctc
      421 gtcgctgatc cgcccgtaac ggtaaagctg aaaataactt ccatgagagg gaagggaagt
      481 aaaccgcgtc tgttccatca gttgcgcaaa ataatcccgc ttcttctgca tgaaggcgcc
      541 caatgagaga taactgtcct tgttcgccag gtatttcgcc aggccgaatt gcgacggagc
      601 gtgacagctg aagcagttga actggtgcac tttccggaat tccttcatca gtgcgggcga
      661 ggaaacgcta tatcccagct tccagccggt gcagtggtag atctttccga acgagaagga
      721 aacaaagctg cgttccagca ggtccgggta ccggagcatc gactgatgcg ggatccggtc
      781 gaagatcagg tgctcatata cttcgtcgga atggatgagg atgccggtgt cgcggacgat
      841 agcccgcagc gcttcgatgt cttccggcaa gagcacggcg ccggtcggat tgtgggggga
      901 attcagcagg atcattttgg tccgggcggt cacccggcgc cgcacttcct cccagtcgat
      961 ccggtaatcg ggaaaccgga gcccgatccg gaccggtgtg gccccattga gttcgatgga
     1021 cgggatataa ctgtcatagg ccggttcgaa aacaatcact tcatcaccct ggcgcaagac
     1081 cgtcgtcagc gcggtataga tggcgtaggt tccgccgggc gtaaccgtga tctgcgtttc
     1141 cggatcgatt gcagcgccgt agagcccggc gatcttcgcc gccagcactt cccgcaggct
     1201 tgcgaggccg ttcatgtgca cgtactggtt atgcccttcg cgcatggcct gtgcgaccag
     1261 cgagatcagc tccccgctca tgggaaaatc cggaaagccc tggccgaggt taacggcgcc
     1321 gagctccgcg gccagcgaac tcatcaccgt aaagatggtc gttccgacct gcggcaattt
     1381 gctttcaatc atgcgcttca tctgatctcg atgttttcct ggcgtacggg cggctcgcca
     1441 tgcagccgca gataaacctg cgccactgtg acaacgtctt tctgacagta ggtgacgatc
     1501 cgcggcaggt cgcgctgaac ccagtaaacg tcgccgacct gactgccgtc gatatcatcc
     1561 ttcggcgtgg gaattccgag ggtattcgcc agcaggtgca atgaaatata acacttgtat
     1621 tcgccaaatt tccatagctc catggtatcg aggtgcggga cctcccaggg cttctttccc
     1681 gccagattca acacggacgg caggttcatc ccgtggatga ccatccgccg gcagagatag
     1741 gggaaatcaa agtccttgcc attgtgggcg cagaggtact tctggccctc ggatccccat
     1801 ttatgcagca ggtcgtagaa ccgcatcaac aggtcctttt catcatcgcc ataaaaggac
     1861 ttcagcagca gcttgcggcc cgccggcggc ccggataaca cgccgcagct cacgcagatg
     1921 accttgccga actccgcata gatggcggcc cggccataca gttcttccgg cgttaccggc
     1981 gctttgtccc ggacgaggct gtccgccttg tgacgccata actcctgcca atcttccgga
     2041 acctcgtgga agctggaata ctgcgacacc gtctcgatgt ccagaaaaag gatattatga
     2101 atgggaaaag ctgacatgaa aaggcagccg ttatgcggcc atcacctaaa gatactgatc
     2161 tcccttgtta cgcctcacct cggcgacata ttccttgatc tcctgttcct tttcacgttt
     2221 gcagatcatc agcacgtcgg acgtgtcgac gacgatgaaa ttgtccaggc cctgcagcag
     2281 gagcaatttt tcatttgggg cgtggaccat gcagcgggtg gcgtcgatca cgatgacgtt
     2341 gtcgcccgca accgcatttt ccaggtagtc ctttt
//
LOCUS       DATGKE010000365         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_626269, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000365 DATGKE010000000
VERSION     DATGKE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626269"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VKU83_06280"
     CDS             complement(<1..425)
                     /locus_tag="VKU83_06280"
                     /inference="COORDINATES: protein motif:HMM:NF028471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5118 domain-containing protein"
                     /protein_id="HLI93194.1"
                     /translation="MRRSLTVLLFVTGMSAAVAQRPGNASQPGANPAAGPGQKPAGRT
                     EAPKPYKEVITDKAISHTGLFTVHKVEDKWYFEIPDSIFGRDILVSTRYSRTAAGGNY
                     GGEQVNVQTIRWEKGPSHEVFLKVLTIVNVADDSTQPIA"
     gene            699..1028
                     /locus_tag="VKU83_06285"
     CDS             699..1028
                     /locus_tag="VKU83_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93195.1"
                     /translation="MNANTTPKRFLSVRLTSEELEEVYQHSKNSSCRTLTEYVRKVLT
                     KKPVVIKVRNESQDGLLSAMAGIKDRLDQLADRAQGRNEAEMLREIAEIRTLAKETYE
                     RYCGSPV"
     gene            complement(1025..>2259)
                     /locus_tag="VKU83_06290"
     CDS             complement(1025..>2259)
                     /locus_tag="VKU83_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLI93196.1"
                     /translation="VYGNTRYSVQRAEEAPGEAGNPIPRKAGQRSPIKYVFYVLKENR
                     TYDQILGDMPEGNGDTSLCLFGARITPNAHAIAADFVLLDNFYVDAEVSADGHNWSMA
                     GYATDFTEKTWPSNYSGRGGNYDFDGQRPVANPTKGFIWNYCQRAGVSFRNYGEFEDN
                     GPPTLDVLKEAAHYCIPYVGWNLDTQDVRREQVFEQDFDSLVRIGAVPHFSTVYLPND
                     HTSGLEKGAYTPVAHIADNDLALGRLVDHISHSPIWPETAIFVLEDDAQDGPDHVDAH
                     RSPAYVISPYIKRHSVDHTMYSTTSILRTMELILGLPPMSQYDAAATPLYRSFTATPD
                     TGAFTAIPARVDINQRNSATSESAVLSSHFDLRRADAVPDALLNEVIWKAVKGENSIP
                     PAPRRGAFVKLTAKDRDD"
BASE COUNT          472 a          624 c          669 g          494 t
ORIGIN      
        1 tgtgcgatag gctgcgtcga atcgtccgcc acgttgacga tggtcaggac tttcaggaag
       61 acctcgtggc tgggcccctt ctcccagcga atggtctgga catttacctg ctcgccgcca
      121 tagttgccgc cggcagcagt cctgctgtag cgcgtgctca cgaggatatc gcggccaaag
      181 atggaatccg ggatctcgaa ataccatttg tcctctactt tatgcaccgt gaacaagccg
      241 gtatggctga tggccttgtc ggtgatgact tccttatacg gtttgggcgc ctcggtgcgg
      301 ccggccggtt tctggccggg accggcagcg gggtttgcgc cgggctgact ggcgtttccg
      361 gggcgttggg ctacggcagc tgacatgccg gttacgaata gcaacacggt tagcgatctt
      421 ctcataagtt tgattctgct ttgtttaaga taataggctg aaaaagaacc cagaccgatg
      481 aaaaatgggc tggcggctga aattaaggaa gagggggact atgcctgcat taaaatatga
      541 cgaatgggaa aagtaggaca gcgggcggat taaaaagcgg caaaatataa aataatccta
      601 aaattggccg cttccgccat ttccgttttg agccgatttt tgcttctatt atcttcattc
      661 ccgttaatca aaacctttac atttcaaacc tcgtccagat gaacgccaac actacgccga
      721 agcgtttcct ctccgtacgg ctgacttccg aagagctcga agaagtatac cagcactcta
      781 aaaattccag ctgccgcact ctcaccgagt atgtcaggaa ggtcctcacc aaaaaacccg
      841 ttgtgataaa agtccgcaat gagtcccagg acggcctgtt gagcgccatg gccggcatca
      901 aggaccgcct cgaccagctt gcggaccggg cccagggcag gaacgaagcc gagatgcttc
      961 gggaaatcgc cgagatcagg acgctcgcta aagaaaccta cgagcgctac tgtggctccc
     1021 cggtttaatc atccctatct tttgcagtca gttttacaaa tgcgccgcgt cgcggggcgg
     1081 gaggaatact gttctcgcct ttcaccgctt tccagatgac ctcgttgagg agggcatcgg
     1141 gaaccgcgtc cgcgcgccgc aggtcgaaat gggaagacag gaccgccgat tccgacgtcg
     1201 cggaatttcg ctggttaatg tccacgcggg ccggtatcgc ggtaaaggcg ccggtatccg
     1261 gcgtggccgt aaaggagcga tagagcggcg tggctgccgc gtcgtactgg ctcatcggcg
     1321 ggagtccaag gatcagctcc atggtccgca ggatcgatgt ggtcgaatac atcgtatggt
     1381 cgacactgtg gcgtttgata tagggactga tgacataggc gggcgagcgg tgcgcatcga
     1441 cgtggtccgg gccgtcctgc gcatcgtctt ccagcacgaa gatcgcggtc tccggccaga
     1501 tcgggctgtg ggagatgtgg tctacgagcc ggccgagcgc caggtcattg tccgcgatat
     1561 gtgcgaccgg ggtataggcg cctttttcca atccactggt atggtcattc ggcagatata
     1621 ccgtgctgaa atgcggcacg gcgccgatcc ggaccaggga atcgaaatcc tgctcgaaga
     1681 cctgttcgcg gcggacatcc tgggtgtcga ggttccatcc cacataagga atacagtagt
     1741 gtgccgcctc cttgaggaca tccagggtcg ggggtccgtt gtcttcgaat tccccgtagt
     1801 tgcgaaagct gacgcctgca cgctggcaat aattccagat gaatcccttt gtcggattgg
     1861 cgacggggcg ctggccgtcg aaatcgtaat tgccgccccg gccgctgtag ttactgggcc
     1921 aggtcttttc ggtgaagtcc gtggcatagc cggccatcga ccagttatga ccgtcggcgc
     1981 tgacttccgc gtcgacgtaa aaattgtcca gcaggacgaa atcggcggcg atcgcgtgtg
     2041 cgttcggcgt gatccgcgcc ccgaaaaggc aaagactggt atcgccgttg ccttccggca
     2101 tgtcgccgag gatctggtcg taagtacggt tttccttcag gacataaaac acatatttga
     2161 tcggcgagcg ctgccccgcc ttccgcggaa tcggattgcc cgcctcgccc ggagcctcct
     2221 ccgcccgctg caccgaatag cgcgtgttcc cgtagacct
//
LOCUS       DATGKE010000366         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1384594, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000366 DATGKE010000000
VERSION     DATGKE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384594"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VKU83_06295"
     CDS             <1..962
                     /locus_tag="VKU83_06295"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLI93197.1"
                     /translation="DPFDIGVIWGSGQGGMGTFEEQVEEYAEGDGTPRFSPFFVPKLI
                     ANMASGMISIKYRLMGINYTTVSACSTSNTAFMDALTYIRLGKAKVIVAGGSEAPITE
                     AGIGGFNAMKAMSTRNDDPEGASRPFDVGRDGFVMGEGAGALILEEYSHARARGARIY
                     AELAGAAMTADAYHMTHTHPEGAGAFRAMQDALRDANLDPGQVDYLNVHATSTPVGDL
                     SEILAVTRLFGAAPENLTISATKSMTGHLLGAAGAVEAIASILAIRDGIIPPTINTRH
                     LEPAIPAGLRILLGEAERREVRVAMSNTFGFGGHNGIVVLKKV"
     gene            1011..1421
                     /locus_tag="VKU83_06300"
     CDS             1011..1421
                     /locus_tag="VKU83_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLI93198.1"
                     /translation="MKKLHIVLLILILTSVAVLVSFMGNLSTYETIASAKQKPGRTVT
                     IIAHIDKSQPIEYDPAKDPNYLSFHITDTLGSSAKVVYHSEKPYDLEKAERITLKGKM
                     EGDVFQVANGGILLKCPSKYKDDPRSNQKALTEN"
     gene            1431..>3210
                     /gene="ccsA"
                     /locus_tag="VKU83_06305"
     CDS             1431..>3210
                     /gene="ccsA"
                     /locus_tag="VKU83_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLI93199.1"
                     /translation="MQFIGEHLLPGKIGHFFALLFFCAALVAVYAFFRSATARQPEEE
                     KSWRRMARGAFYIDVAAVFGVLLTIIYIVSNHLFEYNFAWEHSSRSMPLQYLFSCIWE
                     AQEGSFLLWTIWVGVLGLILIRKARKWEGPVMTVISFVHVCLATMIIGMYFFGYKVGF
                     NPFILTRQMDWAVNLPFVQRADYLSFPQFQDGQGLNALLQNYWMTIHPPILFLGFSST
                     VVPFAYAIAGLWKRQWDWTRAALPWTLFSACILGTGIMMGAAWAYESLNFGGYWAWDP
                     VENASLVPWLVMIAGLHTMVIYNATGHSLRSTNFFLITQFILILYSTFLTRSGVLGDT
                     SVHAFVDSGMNLQLGLFVLVFLLPAYVLFGVRYKAIPHIVKEEATDSREFWMFIGSLV
                     LFLSALFIIVATSLPVINKVTKTKLALGDQEFAYNRIEVFIAILVGVLSAVTQYLKYK
                     GTGWNNFAKKIWRPAAVALVVSGLVSAFGGIDYERYGPGFLGAIHIALFAAIFAVVAN
                     AGYIRIGLKGKLRAAGPAIAHMGFGLMLVGILISSAKKRVLSYNTTGIVLPFSPESKE
                     DPMENLTLLKGVPTDMGAYTATYIDND"
BASE COUNT          657 a          916 c          897 g          740 t
ORIGIN      
        1 tggacccatt cgatatcggc gtcatctggg gatcggggca gggtgggatg ggcaccttcg
       61 aggaacaggt ggaggagtac gccgaaggcg atggcacccc gcggtttagc cccttctttg
      121 tcccaaagct cattgccaat atggcgtcgg gaatgatctc cataaaatac cggctgatgg
      181 gcatcaacta caccaccgtg tcggcctgtt ccacctcaaa cacggcgttt atggacgcgc
      241 tgacctatat ccgcctggga aaagcaaagg tcatcgtcgc tggcggctcg gaagctccca
      301 tcacggaagc cgggatcggc ggattcaacg cgatgaaagc catgtcgacc cggaatgacg
      361 accccgaagg ggcttcccgg ccctttgacg tcgggcgtga cggattcgtg atgggcgaag
      421 gggcgggggc gctcattctc gaggaataca gccatgcccg ggccaggggc gcgcggatct
      481 atgctgagct ggcgggcgcc gcgatgacgg cggatgccta tcatatgacc cacactcatc
      541 ccgaaggggc gggcgctttc cgggccatgc aggacgcgct gcgggatgca aacctcgatc
      601 ccgggcaggt ggactatctg aatgttcacg ccacttcgac gcccgtcggg gacctcagcg
      661 aaatcctggc ggtaacccgg cttttcggag ccgcgccgga aaacctgacc atcagcgcca
      721 ccaaatccat gaccggacac ctgctgggcg cggcgggagc cgtagaggcg attgcgtcga
      781 tcctggccat cagggacggc attattcccc ccacgatcaa tacgcgccac ctggagccag
      841 ccattcccgc ggggctcagg atcctgctcg gagaagccga gcgccgggag gtccgggttg
      901 ccatgagcaa taccttcggg ttcggcggcc acaacggcat cgtcgtgctc aaaaaagtat
      961 agcggccaat ctcgcggttt ttgacctaac tttgcggcca caagaaagtt atgaaaaaac
     1021 ttcacatcgt tctattgatc ctcatcctga cctccgtcgc cgttctggtc agcttcatgg
     1081 gaaacctgtc gacgtatgag acgatcgctt cggccaagca gaaacccggc aggaccgtga
     1141 ccatcatcgc gcatatcgac aagagccagc ctatcgagta cgatcccgca aaggacccca
     1201 attacctgag ctttcatatc accgatacac tcggcagttc ggcgaaagtg gtctatcatt
     1261 cggaaaaacc ttatgatctg gaaaaagcag agcgcatcac actgaaggga aagatggagg
     1321 gcgatgtctt ccaggttgcc aacggtggga tcctgctaaa atgtccttcg aaatacaagg
     1381 atgacccccg cagcaatcag aaggcgctta ccgaaaacta aaatccagtc atgcaattca
     1441 tcggcgaaca tctattgccc ggtaaaattg ggcatttctt tgcattactc ttcttctgcg
     1501 ctgcactggt cgccgtttac gctttcttca ggtcggcgac tgcccggcag cccgaagaag
     1561 aaaagagttg gcgacggatg gcgcggggcg ctttctacat cgacgttgca gcggtcttcg
     1621 gcgtattgct gaccatcatc tatatcgtat cgaaccatct tttcgaatac aactttgcct
     1681 gggaacattc gagcaggtcg atgcccctgc aatatctttt ttcgtgcatt tgggaggcgc
     1741 aggagggaag ttttttgctt tggacgatct gggtcggggt actcggcctg atcctcatcc
     1801 gcaaggcccg taaatgggaa ggcccggtca tgaccgtgat cagcttcgtt catgtttgcc
     1861 tcgccacgat gatcatcgga atgtatttct tcggttataa ggtcggcttc aatcctttta
     1921 tccttacccg gcaaatggat tgggccgtga acctgccttt tgtgcagcgc gcagattacc
     1981 ttagcttccc gcagttccag gatggccagg gtctcaatgc cctgctgcag aattactgga
     2041 tgacgatcca tcccccgatc ctcttcctgg gattttcatc tacggttgtg ccttttgcct
     2101 acgcgatcgc cggcctttgg aaacggcagt gggactggac ccgggccgcc ctgccatgga
     2161 ccctcttttc cgcctgcatc ctcggtaccg gcattatgat gggcgctgcc tgggcttacg
     2221 agtcgctgaa ttttggcggc tactgggcct gggatccggt ggagaatgcg tcgctggtac
     2281 cctggctggt gatgatcgcc gggctgcata cgatggtgat ctataacgcc accggtcatt
     2341 cgctccgatc gaccaatttc ttcctgatca cgcaattcat cctgatcctg tactccacgt
     2401 tccttacccg cagcggtgtg ctgggggaca cctcggtgca tgccttcgtc gattcgggta
     2461 tgaacctgca attgggcctg ttcgtccttg ttttcctttt gccggcctac gtcctttttg
     2521 gcgtccgcta caaagccatt ccgcatattg tcaaggaaga ggccaccgat tcccgtgaat
     2581 tctggatgtt catcggatcg ctggtgctgt ttctctctgc gctgttcatt attgtggcta
     2641 cctcgcttcc cgttatcaat aaggttacaa aaaccaaact cgccctcggc gaccaggagt
     2701 ttgcctataa ccggatcgag gtctttatcg ccatcctggt tggcgtgctg tccgcggtga
     2761 cccaatacct gaaatacaaa ggcacgggat ggaataactt cgcgaaaaag atctggcgtc
     2821 cggcagcggt ggccctcgtc gtctccggcc tggtcagcgc cttcggcggc atcgactatg
     2881 aaagatatgg cccgggtttt ctgggcgcca tccacattgc gctttttgcc gcgatttttg
     2941 ccgtagttgc aaacgccggt tatatccgga tcggcctgaa gggcaaattg cgggctgccg
     3001 ggcctgcgat cgcgcacatg ggcttcgggc tgatgctcgt gggcatcctg atctcgtctg
     3061 ccaagaagcg cgtcttgtct tataacacca cgggaatcgt gctgccgttc agtccggagt
     3121 cgaaagaaga tccaatggaa aacctgacac tgctgaaagg ggtccctacc gacatgggag
     3181 cctataccgc gacttatatc gacaatgatt
//
LOCUS       DATGKE010000367         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1552588, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000367 DATGKE010000000
VERSION     DATGKE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552588"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VKU83_06310"
     CDS             complement(<1..1299)
                     /locus_tag="VKU83_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93200.1"
                     /translation="MQFRKIWSAAAPVFLLLLDHSATAQNQTADSLISQISSGPAVTY
                     SIQQCIDSALTNNANVKTAEFIARTAHNGYVQGIGNMLPSLSANLNFYNNGGKTVNPV
                     TYQYVTENFNQGTGGIGGQLVLFNGFSLLNSLKQMSLAYQADMKDWQYQKDVITVTVI
                     QDYFSILGTEEQLALAQKQAADYRRRTDLMAVQDSLGSIPPSQYTDQKAALASSELTI
                     VNTKNLLEQAKAKLAMDMNIPYSPNMEVAQVNVDTNPTLYAASVDQVYQNARHHIAEI
                     AAVDLHLKAAQKGVASTRGALSPQLTLLYGLNNNYSTASNVKFGTQFQNDYNYAFGLG
                     MNIPILNGLRFRTAYKNAQVARDAALFNQKTINNSLRQAVESSYVNMVQNFRAYNVTS
                     REVENFEESYREAAIRFDNGALSSIDYIIYNTNKNNAELSL"
     gene            complement(1337..1993)
                     /locus_tag="VKU83_06315"
     CDS             complement(1337..1993)
                     /locus_tag="VKU83_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93201.1"
                     /translation="MKRISKWVNVGGKPNFILKDINLHIKEGEFISIMGPSGSGKSSL
                     LNIIGMLDDSSEGEYMFLGQPVHNLKEKQRSQLYKQYIGFVFQAYHLIDELTVYENIE
                     TPLIYQNVKTSERQAMVADILDRFQIVGKKGLFPTQLSGGQQQLVGIARALIGKPKLL
                     LADEPTGNLNSKQGEEIMELFKQLNKEGVTIIQVTHSEKNAAYGTHIINLLDGRIELT
                     "
     gene            complement(2169..>2566)
                     /locus_tag="VKU83_06320"
     CDS             complement(2169..>2566)
                     /locus_tag="VKU83_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93202.1"
                     /translation="KDRLMGDAIGFFTGLAILLATLGLIGLTLFTIERRTKEIGIRKV
                     LGAGNRSILKLITGEFARLALIASAVALPVGWWLAARWLNHFAYRVSIQAWTVLGTET
                     LIGGIALAVICGLTLRALLANPVANLRSE"
BASE COUNT          528 a          637 c          717 g          684 t
ORIGIN      
        1 caggctcagt tcggcgttat tcttgttcgt attgtaaatg atgtagtcga tggagctgag
       61 tgcgccgttg tcgaatctga ttgccgcttc gcggtacgat tcttcgaaat tctcgacttc
      121 gcgcgaggta acgttgtagg ccctgaagtt ttgtaccata ttgacgtagc tcgattcgac
      181 ggcctggcgc aagctgttat tgatcgtctt ctgattaaac aatgccgcgt cgcgcgcaac
      241 ctgtgcattt ttgtatgcgg tccgaaagcg cagtccgttc agaataggga tattcatgcc
      301 caatccaaag gcatagttgt aatcgttctg gaattgggtg ccgaacttaa cgttgctggc
      361 cgtcgagtag ttattgttga ggccatagag cagggttaac tggggactaa gcgctcccct
      421 ggtggaagcc actcctttct gggcggcttt aaggtgcaaa tcgacggctg cgatctctgc
      481 gatatgatgc cttgcgtttt ggtaaacctg gtcgaccgaa gcggcataca gggtagggtt
      541 tgtgtcgaca ttcacctggg ctacttccat attcggcgag taagggatat tcatatccat
      601 agccagcttg gccttggcct gttcgagcag attctttgtg ttgacgatgg ttagctcgct
      661 gctggcgaga gccgcctttt ggtccgtgta ttggcttggc ggaatggaac ccaggctgtc
      721 ctggacagcc atcaggtcgg tacggcgccg gtagtcggcc gcctgtttct gggcgagggc
      781 caactgctct tccgtcccaa ggatggaaaa atagtcctgg ataaccgtaa cggtaatcac
      841 atctttctgg tactgccagt ccttcatatc ggcttgatag gcgagggaca tttgcttaag
      901 cgaattgaga aggctgaaac cgttaaaaag taccagctgc ccgccgatcc ctccggtgcc
      961 ctgattgaag ttctccgtta catactggta cgtgaccggg ttaacggttt taccgccgtt
     1021 attatagaag ttgagattcg cgcttaatga cggcagcata tttccgatgc cctgaacata
     1081 accgttgtgg gcggtgcggg cgataaattc ggctgtctta acgtttgcat tgttggtcag
     1141 ggcgctgtcg atacactgct ggatgctgta ggtcacggca gggccggagc tgatctgact
     1201 gataagggaa tcggcggttt ggttctgcgc ggtagcggaa tgatccagga gcagcaagaa
     1261 gacaggcgct gctgctgacc atatttttcg aaattgcatg ttcattgaga ttttgagctt
     1321 cgatggttgt ttgcgatcag gtcagttcga tccgtccatc gagcaggtta atgatatggg
     1381 ttccataggc ggcattcttt tcagagtggg taacctggat gatggttacg ccttccttat
     1441 tgagttgttt gaatagttcc atgatctcct cgccctgctt cgaattgagg ttgcctgtcg
     1501 gctcatcggc aaggagcagc ttgggtttgc cgatcagcgc acgcgcaatg cctaccagct
     1561 gttgctggcc cccggaaagc tgggtcggaa agagcccttt cttgccgacg atctggaagc
     1621 ggtccaggat atcggcgacc atggcctgcc gttccgacgt cttgacgttc tggtaaatga
     1681 gcggtgtttc gatgttttcg tatacggtga gctcgtcgat caggtgataa gcctgaaaga
     1741 cgaagccgat gtattgtttg tatagttggg accgctgttt ttctttcaga ttgtgcacgg
     1801 gttgacccag gaacatatat tccccctccg aagagtcgtc cagcatgccg ataatgttca
     1861 gaaggctgga tttaccggag cccgacggac ccatgatcga gatgaactcg ccttccttga
     1921 tatgaaggtt gatgtccttg aggatgaaat tcggcttacc gccaacgttg acccattttg
     1981 agatgcgctt catttctatc ataaatctat attagcgttt actgccggcc gccttgtttc
     2041 tttcaaccac atgccagcat ttaatgtctt gaatatcatt ttgatataat gcaattccac
     2101 cagctgtagt ccgctttcga tacaagagct gtccggtttc gatacagcct gtcccctccc
     2161 cgtcgtgctc actcggaccg caggttagct accgggttgg ccagcaaggc gcgcaacgtg
     2221 agcccgcaga tgactgccag ggcgatgcct ccgatcaggg tctcggtacc cagcacggtc
     2281 caggcctgga tggagaccct gtaggcgaaa tggttcaacc accgggcggc tagccaccat
     2341 cccacgggca gggcaaccgc tgaggcaata agtgcgagcc gggcaaactc gcccgtgatc
     2401 agtttcagga tactcctatt tcccgcgccc agcacctttc ggatgccaat ctcctttgtc
     2461 cggcgttcga tcgtgaataa ggtcagtccg atgaggccga gtgttgcaag aaggatcgcc
     2521 agcccggtaa aaaagccgat cgcgtcgccc atcagccggt cctttt
//
LOCUS       DATGKE010000368         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1458675, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000368 DATGKE010000000
VERSION     DATGKE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458675"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..452
                     /locus_tag="VKU83_06325"
     CDS             57..452
                     /locus_tag="VKU83_06325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HLI93203.1"
                     /translation="MTIFSKIIAGDIPSYKVAENERFFAFLDIFPLREGHTLVIPKLE
                     IDRFFDLGDDYLAGLMLFAKPIALAIEKAFHCNRCGLSVVGLEVPHAHLHLIPIDRAD
                     DLNFTRPKLKPTPDQLRLAQEKILRHLHP"
     gene            472..1770
                     /locus_tag="VKU83_06330"
     CDS             472..1770
                     /locus_tag="VKU83_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLI93204.1"
                     /translation="MPADIHLGPADQIKAMQEQKLQELLAYTARQSPFYRELFSKHSI
                     DVFSIRTLEGLAVIPPTHKEDLQRRSEDFLCVGRGRIAEYTTTSGTLGAPVTIALTAG
                     DLERLAYNEHRSFLCAGGTPADIYQLMLTLDRQFMAGIAYYSGIRRLGASVIRVGPGV
                     PALQWETIRRLRPTVLVAVPSFLLKLIGYAREYHIDLDRSSVKSAICIGENIRHADLS
                     LNILGRKITDAWNIRLYSTYASTEMQTAFTECGEGRGGHLQPELLIVELLDEGDRPVG
                     AGLPGEVTITTLGVEGMPLIRYKTGDICTWFDEPCPCGRKTLRLSPVIGRRGQMIKFK
                     GTTLYPPALFELLNEMEEVVDYVVEVYPNDLGLDEILLHLVPASRSEDCDHRIRAYLQ
                     ARLRVSPHVSYHSLQELQKMQFPEAGRKAIKFLDRRTDYK"
     gene            1781..>2536
                     /locus_tag="VKU83_06335"
     CDS             1781..>2536
                     /locus_tag="VKU83_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HLI93205.1"
                     /translation="MIAVGQKPLSILDFSDILYQKRDFDLDEAAMEKVAANFRFLAEF
                     SADKLIYGINTGFGPMAQYKVGEGNLLQLQYNLIRSHCSGSGALLSPELVRATMITRL
                     NSLIQGYSGVHPDTVSLLRELIARNILPCIYEHGGVGASGDLVQLAHLGLTLIGEGEV
                     MYEGTVQPTAAVFKRLGITPLSVRIREGLAILNGTSAMTGIGLVNILRGRRLVEWAAF
                     LSALTNEIVGAFDDHFSYELNIVKHHPGQNKIAA"
BASE COUNT          554 a          726 c          697 g          559 t
ORIGIN      
        1 gcacaggtgc aggttccggc gggcacgctg caattcaaaa ttgaaaacat aacagtgtga
       61 cgatcttctc caagataatt gctggagata tcccttctta caaggtggcg gaaaatgagc
      121 ggttctttgc cttcctggat atttttcccc ttcgcgaagg ccacacgctg gtcataccca
      181 aactggaaat tgaccgtttc ttcgatcttg gggacgatta cctggcaggt cttatgcttt
      241 tcgccaaacc gatcgcgctg gccatcgaga aggcattcca ttgcaaccgc tgcgggctct
      301 cggtcgtggg cctggaggtt ccccatgctc atcttcatct cattccgatc gaccgcgcgg
      361 acgatctgaa ctttacccgg ccgaagctaa aaccgactcc cgatcagctc cggttggcac
      421 aggagaaaat cctgcgacat ctacatccat gacagggggt tatcagactt tatgcccgca
      481 gacatccatc tcggacccgc cgatcagata aaagcaatgc aggaacaaaa actgcaggag
      541 ctgctcgcct atacggcccg gcagtcgcct ttttaccgcg agctctttag caaacacagc
      601 atcgatgtct tttcgatccg gacccttgag ggcctggcag tgatacctcc gactcataag
      661 gaagacctgc agcgacggag tgaggatttc ctctgcgttg gcagggggcg gatcgccgaa
      721 tacactacca cgtccggcac gcttggcgcg cctgtcacca tagcccttac ggcgggagac
      781 ctcgaacgcc tggcatataa tgagcatcgt tcctttctgt gcgccggcgg aacaccggcc
      841 gatatttacc agctgatgct gaccctggac cggcaattca tggccgggat cgcttattac
      901 tccggcatcc ggcggcttgg cgcttccgtc atccgtgtag gccccggcgt ccccgcgctc
      961 caatgggaga ccatccggcg gcttcgcccc accgtcctgg tcgccgttcc ttcgttttta
     1021 ctgaagctga tcggatatgc cagggaatac catattgacc tcgaccggtc gtccgtgaaa
     1081 tcggctattt gtatcggcga gaatatccgc cacgccgatc tttcactgaa tatccttggc
     1141 agaaagatta ccgatgcctg gaatatccgg ctctattcta catatgccag taccgagatg
     1201 caaaccgcat tcaccgagtg cggtgagggg agaggcggtc acctgcaacc cgaattgctc
     1261 atcgtggagc tgcttgacga aggtgaccgg ccggtggggg ccgggcttcc gggcgaagtg
     1321 acgattacta cgctgggcgt tgaaggcatg ccgctgatcc ggtacaagac gggggatatt
     1381 tgcacctggt tcgacgaacc ttgcccgtgt ggacggaaaa cccttcgcct gtcaccggtc
     1441 atcggccggc gcgggcagat gatcaaattc aagggaacca ccttgtatcc gccggccctc
     1501 ttcgagctgc tgaatgaaat ggaagaggtt gtcgactatg tcgttgaagt ctatccaaac
     1561 gacctgggcc tcgacgagat cctcctgcac ctcgtccccg cgagccggag cgaggattgt
     1621 gatcatcgca tccgcgctta tttacaggcc aggttgagag tgagcccgca tgtttcctat
     1681 catagcctgc aagagctcca aaagatgcaa tttccggaag cgggcagaaa agcaattaaa
     1741 tttcttgacc ggagaaccga ttataaataa attgcagccc atgatagctg ttggacaaaa
     1801 gcctctttcg atacttgatt tttccgatat cctttatcaa aagagagact tcgacctgga
     1861 cgaggcggcg atggaaaagg tagcggccaa ttttcggttc ctcgccgaat tttcagccga
     1921 taaattgatc tatggcatca acaccggatt cggcccgatg gcccaataca aggtggggga
     1981 agggaatttg ttgcaattgc aatacaatct cattcgcagc cactgctcgg gaagcggtgc
     2041 gcttttgtcc cccgagctcg tccgggcgac gatgatcacg cggctgaaca gccttatcca
     2101 gggctactcg ggcgtgcacc cggacaccgt cagcctcctc cgcgagctga tcgccagaaa
     2161 tatccttccg tgcatctatg aacatggggg cgtcggcgca agcggcgacc tggtgcagct
     2221 tgcccacctg ggcctgaccc tgatcggcga gggagaagtc atgtatgaag ggacggtcca
     2281 gcccaccgca gcggtcttta aaaggctggg catcacgccg ctgtccgtcc gtatccggga
     2341 agggctggcc atattgaacg gcacctcggc catgacgggg atcggtctcg ttaatatcct
     2401 ccgcggccgg cggctggtag agtgggctgc ttttctttcc gcactcacca acgagatcgt
     2461 gggcgccttc gacgaccatt tttcctacga gctgaatata gtgaagcacc atccggggca
     2521 gaacaagata gcggcg
//
LOCUS       DATGKE010000369         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_730803, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000369 DATGKE010000000
VERSION     DATGKE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730803"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VKU83_06340"
     CDS             <1..516
                     /locus_tag="VKU83_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HLI93206.1"
                     /translation="NSSWKYTERMSFDFDFIGIQNYFPVVVKYNPLIPIVQASEVKAA
                     TRKVPQTALGWEINPDSHYRILKRFWLYGGVREIIVTESGAAFRDEAVNGVVDDPARI
                     DYYRGYLLALLRAKKEGVNVKGFFAWTLMDNFEWAFGYNARFGLIHVDFKTQLRTIKS
                     SGYWFRDWLKK"
     gene            complement(560..1462)
                     /locus_tag="VKU83_06345"
     CDS             complement(560..1462)
                     /locus_tag="VKU83_06345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLI93207.1"
                     /translation="MMKNMVSGLLGFLLFTVTACGTGTNGKNNTAVPAAADTTNTARP
                     DATAATLTGGVDTTGSPGGKVPPPSVILGRKEVPILCYHQIRDWKPTDSKVSKDYIMP
                     PADFRAQVKMLADSGYHSVLPDQLYAYLTKGEPLPSKPIMLTFDDTDEDQWMVAWPEM
                     KKYGFRGVFFIMTVSLNRLPHYMTKQQVKELFDAGNVIGSHTWDHHSVKQYKGKDWEI
                     QIDKPTKELEAITGKPIRYFAYPFGLWNRQALPELRKRGFLAAFQLADKMDPTDPLMT
                     IRRIIVGGGWSLKTFSAAIHRDFH"
     gene            complement(1490..1972)
                     /locus_tag="VKU83_06350"
     CDS             complement(1490..1972)
                     /locus_tag="VKU83_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLI93208.1"
                     /translation="MAEDLQLATGSKTDQYRSLLPQIRALVDGEPDRTANLANIVAAL
                     KQQFGWLWVGFYLVKDGELVVGPFQGPVACTRISKGRGVCGTSWLQARSLVVPDVEVF
                     PGHIACSSLSRSEIVVPVFDNGEVVGVLDADSSEPGEFDEEDRHWLEALVADWAAWGK
                     "
     gene            2019..3098
                     /locus_tag="VKU83_06355"
     CDS             2019..3098
                     /locus_tag="VKU83_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLI93209.1"
                     /translation="MSRFTIDPDIARARTPSTDLYTDPALFELCKEKIFAPSWQFIGD
                     TDPVAAAGDAWPFILLEGLLDEPLVLVRNQDGGLRLLSNVCTHRGNLVVNEPCRLTHL
                     HCRYHGRQFGLDGTFRLMPEFRGVKDFPSPADDLHVLPLGQWGKWLFTTLGASPFDAC
                     WQEIRQRLAWLPLDRYVLHAGYNKEYIVHANWALYVENYLEGFHIPFVHAGLNTVIDF
                     GNYTTELFRYSSLQLGIAKDDEDCFELPPDSPDYGKKVAAYYFFVFPNLMFNFYPWGL
                     SVNVVKPLGLSRTRISFLTYIANPAKYDMGAGSGLDTVELEDEEVVEAVQKGVRSRFY
                     SHGRYSVTREQGTHHFHRLIAASIG"
     gene            3101..>4048
                     /locus_tag="VKU83_06360"
     CDS             3101..>4048
                     /locus_tag="VKU83_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLI93210.1"
                     /translation="MPAQLQKTIGLWSATSLVIGSIIGAGIFMRPSTMAGQLGSPTLL
                     LTVWVVGGAVSLIGAMIYAELGAMYPETGGPYLYLQEMYGDLPAFLYGWSSLVVINTA
                     SLASFAFVCADYAGWFIHLPRFSAGTEHFIKWHIPFLADIYPLENAGTKSLAILILAV
                     ITVVNYRSTRVGNGIQVVATTLKALAILLVVGGIMFSGNGHAANFVRNAPGFHGQPWL
                     VFTALMAATSGAFSSYDGWYNINMMAGEMIDPKRNISRSLFLGVGACIVIYLLVNLAY
                     LYVLPVGVMAHSGMVAADAVNAGIGTSGAALVAALIILSC"
BASE COUNT          790 a         1215 c         1125 g          918 t
ORIGIN      
        1 aacagcagtt ggaaatatac cgaacggatg agctttgatt tcgatttcat cggcatccaa
       61 aattattttc ccgtggttgt caaatacaat ccgctcatcc ccatcgtgca ggcttcggaa
      121 gtcaaggctg ctacccgaaa ggtaccacaa acggccctcg gctgggaaat aaacccggac
      181 agtcactatc ggatcctgaa acggttttgg ctatacggtg gcgttcggga aatcatcgtc
      241 acggaaagcg gagcggcatt tcgcgacgag gcggtaaatg gcgtcgtaga tgacccggcg
      301 cggatcgatt actaccgggg ctacctgctg gccctcctcc gcgcaaaaaa ggaaggtgta
      361 aacgtaaaag gattttttgc ctggacgctg atggacaatt tcgaatgggc atttggctac
      421 aacgcccgct tcggcctcat ccacgtcgac ttcaagacgc agctgcgcac gatcaagagc
      481 tccggctatt ggttccgcga ctggctgaaa aaatagaccg ggaccgggcc gcggcggctc
      541 acccgccgcc agccgatgat cagtgaaagt cccggtggat ggccgcgctg aaggtcttca
      601 ggctccagcc gccgcctaca atgatccggc ggatcgtcat gagcggatcg gtgggatcca
      661 ttttgtcggc cagctgaaaa gccgccagaa agccccgctt ccgcagctca ggcaatgcct
      721 ggcggttcca gagcccgaaa ggataggcaa agtaccggat cggcttcccg gtaatggcct
      781 cgagctcctt cgtcggcttg tcgatctgga tctcccagtc tttgcccttg tattgcttga
      841 cactgtggtg gtcccaggtg tggctgccga tcacattacc cgcatcgaaa agctccttca
      901 cctgttgctt cgtcatataa tgcggcaggc gattgagcga aacggtcatg atgaagaaca
      961 cgcccctgaa cccgtatttt ttcatttccg gccacgcgac catccactgg tcttcgtcgg
     1021 tgtcgtcgaa ggtaagcatg atcggcttgc tgggcagcgg ctcgcctttt gtgaggtacg
     1081 catacaattg atcgggaagt acggaatgat agccgctgtc ggcaagcatt ttcacctgtg
     1141 ccctgaagtc tgcgggcggc atgatataat ccttggagac tttcgagtcc gtgggtttcc
     1201 agtcacggat ttggtgatag cacaggatcg gcacctcctt gcgaccgagg atgaccgaag
     1261 gaggcggaac cttgccgccg ggtgatcccg tcgtgtcgac cccaccggta agcgtagccg
     1321 ccgtcgcatc cggccgggcc gtattcgtcg tatctgccgc cgcgggaacg gcggtgttgt
     1381 tttttccatt ggtaccggtc ccgcaggccg taaccgtaaa taataaaaat cccaggagtc
     1441 cgctgaccat attcttcatc atattcaatt gtacgtgttt ttgtaacgat tatttccccc
     1501 aggcagccca gtccgcaacc agcgcctcca gccagtgccg gtcttcttcg tcaaattcgc
     1561 cgggctcgct gctgtcggcg tcgagcacgc ctactacttc cccattgtca aaaacaggca
     1621 cgacgatttc cgaacgcgac aggctgctgc aggcaatatg tcctggaaat acctctacat
     1681 ccggtacgac caggctccgg gcctgcagcc agctcgttcc gcagactccc ctgccctttg
     1741 agatccgcgt gcaagccacc ggtccctgaa atggcccgac gacgagctct ccatccttta
     1801 ctaggtagaa gcccacccac agccagccga attgctgctt cagcgcagcc acgatgttgg
     1861 cgaggtttgc cgtccggtcc ggctcgccgt ccacgagcgc ccggatctgc ggaaggagcg
     1921 agcggtactg atccgtcttg ctgccggttg ccaattgaag atcttctgcc atgagggcaa
     1981 atttacaaac atctgccaac tctttcttaa attacgcaat gtcccgtttc accattgacc
     2041 cggatatcgc ccgtgcacgc acgccttcca cggacctgta caccgatccg gcccttttcg
     2101 aactttgcaa agagaaaatc ttcgctcctt catggcagtt tatcggggac acggatccgg
     2161 tcgcggctgc cggcgacgcg tggccgttca tcctgctcga gggcctgctg gacgaaccgc
     2221 tggttctggt ccggaatcag gacggcgggc tccgtctgct gtcgaacgtg tgcacgcatc
     2281 gcggaaatct cgtcgtaaat gaaccctgcc ggcttacgca tctccattgc cgttaccacg
     2341 gccgccaatt cgggctcgac gggaccttca ggctaatgcc cgagtttagg ggagtgaagg
     2401 attttccgtc gcctgccgac gacctccacg ttcttccgct cgggcaatgg ggaaaatggc
     2461 ttttcaccac gctgggcgcc tcgccgttcg acgcctgctg gcaggagatc cggcagcggc
     2521 tggcctggct gcccctggac cgttatgtgc tgcatgccgg gtacaacaag gagtatatcg
     2581 ttcatgcgaa ttgggcgttg tacgtggaga actacctcga aggttttcat atccccttcg
     2641 ttcacgccgg cctcaacacg gtgatcgatt tcggcaacta cacgacggaa ttgttccggt
     2701 attccagcct gcagctcggc atcgcaaagg acgatgaaga ttgcttcgag ctgccgcccg
     2761 actcccctga ctacggaaag aaagtagccg cgtactactt ctttgtcttc ccgaatctga
     2821 tgttcaactt ctacccctgg ggcctgtccg tcaatgtcgt aaaacccctg ggtctgtcgc
     2881 ggacaaggat cagttttctc acctatatcg ccaatcctgc caaatatgat atgggcgcgg
     2941 gatcggggct cgataccgtc gagctcgagg acgaagaggt cgtcgaggcc gtgcagaagg
     3001 gtgtccgatc gaggttttat agccacggac ggtactcggt tacccgggaa caggggaccc
     3061 atcatttcca ccgcctcatt gccgcgtcaa tcggataatc atgcctgccc aattgcaaaa
     3121 aacgattggt ctctggtccg ccacttccct ggtgatcgga agcatcatcg gcgccggcat
     3181 ttttatgcgg ccctcaacca tggcgggtca gctcggcagc cccacgctcc tgctgaccgt
     3241 ttgggtggtt ggcggcgccg tttcgctgat cggcgccatg atctatgcgg aattgggcgc
     3301 catgtacccg gaaacgggcg gcccctacct gtatctgcag gagatgtacg gggacctgcc
     3361 ggccttcctc tatggctggt catcgctggt ggtcatcaat acggcatccc tggcttcctt
     3421 cgccttcgtt tgcgcagact atgcgggttg gttcattcac ctgccgcgtt ttagtgccgg
     3481 caccgagcat ttcatcaagt ggcatatccc gttcctcgct gatatctatc cgctcgaaaa
     3541 tgcgggcacc aagtcgctgg cgatcctgat cctggcggtc atcacggtcg tcaactaccg
     3601 gagcacccgc gtcggtaacg ggatccaggt tgtcgctacc acgctcaaag ccctggcgat
     3661 cctgctggta gtcggcggca tcatgttctc cggcaacggg catgcggcga attttgtgcg
     3721 caacgccccc ggctttcatg ggcaaccctg gctcgttttt accgcgctga tggcagccac
     3781 gtccggagct ttctccagtt acgatggctg gtacaatatc aatatgatgg ccggcgaaat
     3841 gatcgatccg aagcgcaaca tcagccgaag cctgttcctg ggcgtgggcg cgtgtatcgt
     3901 gatctatctc ctcgtgaacc tcgcctacct gtacgtcctg cccgtcggcg tgatggcgca
     3961 ttcagggatg gtcgccgcag atgctgtaaa tgccggcatc gggacttccg gcgccgccct
     4021 ggtggccgcg ctgatcattc taagctgt
//
LOCUS       DATGKE010000370         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_160594, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000370 DATGKE010000000
VERSION     DATGKE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160594"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VKU83_06365"
     CDS             <1..902
                     /locus_tag="VKU83_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLI93211.1"
                     /translation="ALLLKEAGLPDGVFNVVNGDSAMVEAICDHPGIDAVSFVGSTKV
                     AKIVYRRSTLQLKRCLALGGAKNHLIVLPDAHPGMTAQNVAASMSGCAGQRCMAASAM
                     LGVGAVDQIIRKICDEARRIVPGENLGAVIDRAAKERIERYITEAEQQGATILVDGRG
                     YTVPGKEGGTYVGPTVIDHVRPGMAVATEEIFGPVISILRVDTLDQALEIENASPYGN
                     AASVFTQNGALARYVIDRASAGMVGVNVGVPVPREPFSFGGWNESKFGVGDITGKPSI
                     EFWTQLKKSTVKWNPEAGVNWMS"
     gene            915..2270
                     /locus_tag="VKU83_06370"
     CDS             915..2270
                     /locus_tag="VKU83_06370"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLI93212.1"
                     /translation="MPEPTTLSEAREIVKDNLDYTLFSWSKQKGLDPIAVQRGEGVYL
                     YDYDGKRYLDFSSGLMNVNIGHGDQRVTDAVVRQMQEVCYVTPSCVTRVRGELGKKLA
                     EITPGNLKKTLFTVCGATAIDNAIKLARLYTGRHKIIARYRAFHGASYGAMTAGGDPR
                     KLPADAQQAPNFVHVEDPYCYRCPWSQELSSCHRECVAHIERIIEFEGPSTIAAILME
                     GESGSSGCIKYPPDYLQKIRALCDRHGILLIVDEVMSGFGRTGKWFASELHGIVPDMI
                     ATAKGITAGYIPLGALIVSEAIADHFNDKVLWLGLTYADHPVACAAALEVLRIYEEDG
                     LIENAAAMGRYLDQRIEELKAVHPCIGDWRNTGLLGCLELVRNRKTREPMAPFNARPE
                     EMVVMNQVAARLRQLGLYTFVRWNYVFIAPPLCITREQIDEGLGVIREALSIADAHVI
                     E"
     gene            2276..>2996
                     /locus_tag="VKU83_06375"
     CDS             2276..>2996
                     /locus_tag="VKU83_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLI93213.1"
                     /translation="MRILILLLLFQEIAVAQPKRARDYGVPFDGQPGPVNAITDVPGV
                     TVGQTTLIRGDGPLIKGKGPVRTGVTAILPRGSVFDPVYANWYTLNGNGDMTGTHWVT
                     ESGFLETPILITNTGSVGTVRDAAWQWMDKHQYYTPFYKDYWYAYPVVAETYDGIFND
                     INGQHVTREDVFHALDSARSGPVEEGGVGGGTGMMCYNFKGGIGTASRVLDKKDGGYT
                     VGVLVQCNHGSRGQLTIAGVPV"
BASE COUNT          626 a          876 c          893 g          601 t
ORIGIN      
        1 tagcactgtt gttaaaggaa gccgggctcc ccgatggcgt cttcaatgtc gtgaacggcg
       61 acagcgccat ggtggaagct atctgcgatc atcccggtat cgacgccgtc tcttttgtgg
      121 gttccaccaa agtggccaaa atcgtctacc gtaggtcgac actgcagctg aaacgttgtc
      181 tggccctggg cggcgccaag aaccatctca tcgtgctgcc ggatgcccat ccggggatga
      241 ccgcccagaa tgtcgccgct tcgatgagcg gctgcgccgg tcagcgctgt atggcggctt
      301 ctgccatgct aggcgtggga gcggtagacc aaattatccg gaagatctgc gacgaggccc
      361 gccgtatcgt ccccggcgag aacctgggag cggtaatcga tcgcgccgcg aaagagcgga
      421 tcgaacgtta tatcaccgaa gccgaacagc aaggcgccac catcctggtc gacggccggg
      481 gatacacggt tcccggaaaa gaaggcggca cctatgtagg gcccaccgtc atcgatcacg
      541 tgcggcccgg catggcagtc gctactgaag agatcttcgg tccggtgatc agcattctgc
      601 gcgtggacac cctggaccag gcgttggaaa tcgagaatgc cagcccttac ggcaatgcgg
      661 cctcggtatt cacgcagaac ggcgcgcttg cccgctatgt gatcgaccgg gccagcgccg
      721 ggatggtcgg ggttaacgtc ggcgtccccg tcccccgcga acccttttcc tttggcggct
      781 ggaatgaaag taagtttggc gtcggcgata tcaccggcaa gccctctatt gaattctgga
      841 cgcagctgaa gaaatcgacc gtcaaatgga atcccgaggc gggcgtcaac tggatgagct
      901 aaaaaaataa ccatatgccg gaacctacta ccttgtcgga agcccgagag atcgtaaaag
      961 acaacctgga ctataccctt ttttcctggt ccaagcaaaa agggctcgat cccatcgccg
     1021 tacagcgcgg tgaaggcgtc tatctgtacg actacgacgg taaacgttat ctcgacttct
     1081 cgagcggcct catgaatgtc aatatcggcc acggcgatca gcgggtgacc gacgctgttg
     1141 ttcgccagat gcaggaggtg tgctatgtga cgccgtcgtg tgtcacgcgc gtccgcgggg
     1201 agcttggcaa aaagctggcg gagatcacgc ccggcaacct caagaaaacg ctgttcacgg
     1261 tatgcggcgc taccgccatc gacaatgcga tcaagctggc ccggctgtat accggccggc
     1321 ataagatcat cgcccgctat cgcgccttcc atggggcgtc gtatggggcc atgacagccg
     1381 gtggcgaccc ccggaaactg cctgccgatg cgcaacaggc gcccaatttc gtccatgtgg
     1441 aggaccccta ctgctatcgt tgtccctgga gccaggaact ctccagctgc catcgggaat
     1501 gcgtggcgca tatcgaacgc atcatcgaat tcgaagggcc ttcgaccatc gctgccatcc
     1561 tcatggaagg ggaaagcggc tcatccggct gcatcaagta cccgccggac tatctgcaaa
     1621 agatccgggc gctctgtgac cgccacggca tcctgctgat cgtcgacgag gtcatgagcg
     1681 gtttcggccg gacggggaaa tggtttgcct ccgagctgca tggtatcgtg ccggatatga
     1741 tcgccacggc caagggtatc accgccggtt atatccccct gggggccctc atcgtcagcg
     1801 aggcgatcgc cgaccatttc aatgataagg tgctctggct gggtcttact tatgccgacc
     1861 atccggtagc ctgcgccgcc gcgctcgaag tgctccgtat ctacgaagag gacggcctga
     1921 tcgaaaatgc tgccgccatg ggccgctatc tcgaccaacg gatcgaggag ctcaaagccg
     1981 ttcatccctg cattggagat tggcgtaata ccggtttgct cggctgtctg gaactggtcc
     2041 gcaaccggaa gaccagggag ccgatggcgc ccttcaatgc ccggcccgaa gagatggtcg
     2101 tcatgaatca ggtggcagcc cggcttcgcc agctcggctt gtatacgttc gtacgatgga
     2161 actatgtctt catcgccccg ccgctctgca tcacccgtga gcagatcgat gaaggactgg
     2221 gagtgatcag ggaagcgttg agtattgcgg atgcgcatgt aattgaatga tttgcatgag
     2281 aatattaatc ttacttttac tttttcagga gatcgcagtg gcgcaaccca agcgggcccg
     2341 cgattatggc gtccccttcg acggccagcc cggccctgtc aatgccatca ccgatgtacc
     2401 gggcgtgacc gtgggtcaga cgaccttaat tcgcggcgat ggcccgctca taaaagggaa
     2461 agggccggtc cgcaccggag tgacagccat cctgccgcga ggctcggtct tcgaccccgt
     2521 ctatgccaat tggtataccc taaatggcaa tggggatatg accggtaccc attgggtcac
     2581 cgaatccggt ttcctggaga cgccgatcct catcaccaac accggcagcg tcggcactgt
     2641 ccgtgatgcc gcctggcaat ggatggacaa gcatcagtat tacacccctt tctataagga
     2701 ttactggtac gcctatccgg tggtagccga gacctatgac ggcatcttca acgatatcaa
     2761 tggccagcat gtcacccggg aggatgtctt tcacgccctg gacagcgccc ggtccggtcc
     2821 cgtcgaagaa ggcggtgttg gcggcgggac gggaatgatg tgctataact tcaaaggggg
     2881 tatcggcact gcttcccgtg tgctggataa aaaggacggt ggttataccg tgggggtgct
     2941 ggtacagtgc aatcacggca gccgcggaca gctgaccatc gccggcgttc ccgtcg
//
LOCUS       DATGKE010000371         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_331259, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000371 DATGKE010000000
VERSION     DATGKE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331259"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VKU83_06380"
     CDS             <1..611
                     /locus_tag="VKU83_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93214.1"
                     /translation="TTAKINQVFMRGTFREGLGMVPEIERQLDEYDLFIDRHRILVLN
                     YKIATLYFGAGDYGTSIDYLQRIINDQQDLRNDLQCYARVLHLMAHYELGNFDLMESL
                     TKSVYRFMARRERLTRVEEEMFRFLRTSFQSSRSSLRTEFEKFLETIRRFEGNRYEAR
                     AFAYLDLVSWVESKVYRRPMSEIIAEKYRKSKRRVMPAGVQG"
     gene            complement(602..2071)
                     /locus_tag="VKU83_06385"
     CDS             complement(602..2071)
                     /locus_tag="VKU83_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLI93215.1"
                     /translation="MGNQRRRATGDARDFDAVVVGSGPNGLAAAILLRQQGLSVLLLE
                     AKQTIGGGLRSAELTLPGFVHDICSAVHPLAISSPFLQTLPLAKFGLEFLYPPVAAAH
                     PLDGGAAALLAGTVSDVARTLGPDERAYSKLMGPIVQHWEELLPDLLAPLHLPKDPLM
                     LARFGWEGLPSATWLARRFRTVEARALIAGMAAHGMQPLSNYTTAAIALLFLASGHDK
                     GWPIPRGGSMAISNALGAYYLSMGGKIETGIAVTSFAQLPSSRCVLLDLTPRQLLRLA
                     DSRWSPLYRRQLGKFRYGMGVFKIDWALAGQIPFLSEGCRAAGTVHIGGTFEEIAASE
                     EATAKGILAERPFVLLAQPSVHDSSRAPAGRHVAWAYCHVPNGSQVDMTERIERQVER
                     MAPGFRELILARHVYNTEQLEEYNPNYVGGDINGGVMDIRQFWTRPALKWSPYRTSEK
                     SVYICSASTPPGGGVHGMCGYHAAKRALRDVFGLSPAHP"
     gene            2116..2598
                     /locus_tag="VKU83_06390"
     CDS             2116..2598
                     /locus_tag="VKU83_06390"
                     /inference="COORDINATES: protein motif:HMM:NF025425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HLI93216.1"
                     /translation="MSNVGLFQRASFPLLALLVVLPGRPAAQTVKSLVESQNYVFQAR
                     TAQPMRGPVRNIATPVYSLQVTTDKIVSDLPYFGRAYIAPMDPTTGPLQFTLDHFDYK
                     LTAGRKGGWKVVIKPKNQQDIQEMELNISAAGYASLQVVSTNRDEISFEGVITAPDKR
                     "
     gene            complement(2618..3076)
                     /locus_tag="VKU83_06395"
     CDS             complement(2618..3076)
                     /locus_tag="VKU83_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI93217.1"
                     /translation="MNAIKGAFSISTDPSRLDVRFIHDYLSKESYWATGMPMEKLVTA
                     VEHSMNFGLYHGDRQIGYARIVTDYSRVAYLADVFVIGEYRGQGLAKWLVGEIMRCPE
                     LQGLRRWMLHTKDAHGLYAQLGWSAPTKPETYLERYNPAQEWWLDWEEGG"
     gene            complement(3238..>3426)
                     /locus_tag="VKU83_06400"
     CDS             complement(3238..>3426)
                     /locus_tag="VKU83_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93218.1"
                     /translation="MLKLPSALDRTPCRPFLTTMLTLPRGARDDESRTTPVIVLVWPK
                     PVSVNNPARKKRMICRNL"
BASE COUNT          772 a         1060 c          889 g          705 t
ORIGIN      
        1 tcacaaccgc taagatcaac caggtcttca tgcgcggaac cttcagggaa gggctcggca
       61 tggtgccgga aatcgagcgc cagctggatg aatacgatct tttcatcgac cggcaccgca
      121 tcctcgtgct caactataag atcgccacgc tctatttcgg cgcgggcgac tatggtacgt
      181 ccatcgatta cctccaacgc atcatcaatg accagcagga cctccgcaac gacctgcagt
      241 gctatgcgcg cgtgcttcac ctcatggcgc attatgaatt gggaaacttc gacctgatgg
      301 agtcccttac caagtcagtc taccgtttca tggcccgccg ggagcgcctg acccgcgttg
      361 aagaggaaat gttccggttt ctccgcacct cctttcagtc ctcgcgctcc agcctcagaa
      421 ccgagtttga aaagttcctg gaaacgatcc gccgcttcga aggcaaccgc tatgaagcac
      481 gggcttttgc atatctcgac ctcgtctctt gggtggaaag caaggtttac cggcggccga
      541 tgagcgagat cattgccgaa aaataccgca agagtaagag aagggttatg cccgccggcg
      601 ttcaagggtg agccggtgac aggccaaaca catcacggag cgcccgcttt gctgcgtggt
      661 agccgcacat gccatgcact cccccgcccg gcggagttga agccgaacaa atatagacac
      721 tcttttccga cgtcctgtaa ggcgaccatt tcaaagccgg ccttgtccag aattgccgga
      781 tatccattac cccgccattg atatcgccgc cgacatagtt cgggttatat tcctccagct
      841 gttccgtgtt gtagacatgc cgcgccagga tcagttcccg gaagcccggc gccatccgct
      901 ctacctggcg ctcgatccgc tccgtcatgt cgacctgcga tccgttgggc acgtgacaat
      961 aagcccatgc cacatgtcgc ccggcaggcg ccctggatga gtcgtgaacg ctaggctgtg
     1021 ccagcagcac aaagggtcgt tctgccagga tgcccttcgc cgtcgcttcc tcggaggccg
     1081 cgatctcctc gaatgtcccg ccaatatgaa cggttcccgc tgcccgacac ccttcagaaa
     1141 ggaacggtat ctggccggca agcgcccagt cgatcttaaa gacccccatt ccataacgga
     1201 atttcccaag ctgccgccgg tagagcggcg accagcggct gtcggcaagc cggagcaatt
     1261 gccgcggcgt caggtcaaga agaacgcacc gggacgaagg cagctgcgca aacgaggtta
     1321 cggcgatccc cgtctcgatc ttgccgccca tggacaaata gtaagcccca agcgcattgg
     1381 aaatcgccat gctgccaccc ctcgggatcg gccatccctt gtcatggccc gatgccaaaa
     1441 acagcaaggc gatagcggca gtcgtgtagt tggatagcgg ctgcatccca tgcgccgcca
     1501 ttcccgcgat cagcgcccga gcctcgaccg tccggaacct tcgagcaagc cacgtcgcgc
     1561 tgggtagtcc ctcccaaccg aagcgggcca gcatcaaggg atcttttggt aaatgcaacg
     1621 gtgccagcaa gtcgggaagc aattcctccc aatgctgcac gatggggccc atcagcttcg
     1681 aatacgccct ttcgtcagga ccgagtgtcc ttgccacgtc gctcaccgtc cccgccagca
     1741 gtgcagcggc cccgccgtcg agaggatgcg cggcagcgac cggcggataa aggaactcca
     1801 ggccgaattt cgcgagtggc aaggtctgca ggaaagggga acttatggcc aggggatgaa
     1861 ccgcgctgca aatgtcgtgc acaaaacccg gcagcgtcaa ctcggcagag cgcaaacccc
     1921 cgccaatcgt ttgtttcgct tccagcagca gcaccgacaa cccttgttgc ctgagcaaaa
     1981 tggcggctgc caacccattg ggaccggatc ccaccaccac cgcatcaaaa tcccttgcgt
     2041 cgccagttgc gcgcctgcgc tgattaccca tgaaccaaaa atagctctaa ttcgttatat
     2101 ctgaaatgtg ataccatgag caacgtcggg cttttccaaa gggcctcatt tcccctgctg
     2161 gccttgctgg tcgtccttcc gggccggccg gccgctcaaa cggtaaagag cctcgtggaa
     2221 tctcagaatt atgtcttcca ggcacgcaca gctcagccga tgcgaggccc cgtgcggaac
     2281 attgcaacgc ccgtctattc cttgcaggtt accacagaca agatcgtgag cgacttaccc
     2341 tattttggcc gcgcgtatat cgctcctatg gacccaacga cgggcccgct gcaattcacc
     2401 ctggaccatt tcgattacaa actgaccgcc ggcaggaaag gcggctggaa agtggtgatc
     2461 aaaccaaaaa atcagcagga tatccaggag atggaactca atatttccgc cgccggttat
     2521 gcgtccctgc aggtagtcag caccaatcgt gacgaaatca gctttgaagg agtcattacg
     2581 gctccggata agcgctagat gctgattgag gccggcccta tccgccttcc tcccaatcga
     2641 gccaccattc ttgcgccgga ttgtatcgtt ccagatatgt ttcgggcttt gtcggcgcgc
     2701 tccatccaag ctgggcatag agtccgtgtg catccttcgt gtgaagcatc caccgccgca
     2761 gtccctgcag ttccgggcag cgcattatct caccgacaag ccatttggca aggccctgac
     2821 cgcggtattc cccgattacg aaaacatccg ccaggtaggc cacgcgtgaa taatccgtga
     2881 cgatccgcgc gtacccgatc tgccgatcgc catgatacaa gccgaaattc atcgaatgct
     2941 ccaccgcagt tacgagcttt tccatgggca ttccggtggc ccaataagat tcttttgaca
     3001 aatagtcgtg gataaaccgg acatccagcc tggatgggtc cgtcgaaata gaaaatgctc
     3061 cttttatcgc gttcatgcta tcgcgtgcaa agttaacaaa tccgggcgcc tgcgcaagaa
     3121 gcaataacgc cgggccattt tgcgttttcc agcaagcgca gatttaaggg aaataagcat
     3181 ttattaccta aattgccgga cctaatatct gctttatttt taaaaaagcg accccattca
     3241 cagattacga cagatcattc tttttttcct tgccgggtta ttgacggaaa ccggtttcgg
     3301 ccagaccaga acgatcaccg gggtcgttct cgattcatca tcgcgcgccc ctctcggtaa
     3361 cgtgagcatc gtcgtcagga atggccggca cggcgtcctg tccaacgccg aaggaagttt
     3421 cagcat
//
LOCUS       DATGKE010000372         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_444968, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000372 DATGKE010000000
VERSION     DATGKE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444968"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VKU83_06405"
     CDS             complement(<1..455)
                     /locus_tag="VKU83_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93219.1"
                     /translation="MKRKSFLKRSFLAGASVVAAGAANAASRLVSSGTPGKSAAGEPF
                     HLNYATHDGMFRNSAGPDFIDQIKFAYDQGFRSIEDNGMAQRPPEQQQQIGDTLAKLG
                     MSMGVFVLDKGGNGANSTATGKPENVEIFLKGCRQAVEISRRTNGKLTTV"
     gene            complement(485..1534)
                     /locus_tag="VKU83_06410"
     CDS             complement(485..1534)
                     /locus_tag="VKU83_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLI93220.1"
                     /translation="MKFEVGDKVVVKHSNDDGEVVEIINDKMVVVNVRGVRFPAYTDQ
                     LDFPYFKQFTEKKLFPAEKRLKKYVDQLPPEKPQPGKKKERIAQLATGVWITFLPVFD
                     RDEFDDDVVESLKVYLVNNSALGYHFSYRLNYFGKTDFDLANQILAGHDFYLHDIPFP
                     NLNDNPSLEWEFSLLAPDKARVEYYETTFKPRAKQVFARIGEIRKKGEATFSFKLFDE
                     YPFRPVEEPVDARRLLGESGFRIYDASRARQHLEPARSELDLHAGRLTDNPDALDNFE
                     ILTLQLQTFEKYFDLAIAHHLPSMIVIHGVGTGKLRDEIHEILRTKRQVKSFINRYDP
                     RYGYGATEIFFQYGK"
     gene            complement(1549..>2808)
                     /locus_tag="VKU83_06415"
     CDS             complement(1549..>2808)
                     /locus_tag="VKU83_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLI93221.1"
                     /translation="FLAAQETLSKTAGLTAEAKAPLMDALHTWTSAPTIAGMHFIVNL
                     PAFFIVCVITYITYIGIRESKKTTNAMVIFKLSVVVLVIILGAFFVNTANWHPFLPNG
                     VGGVLKGVSAVFYAYIGFDAISTTAEECTNAQRDLPKGMIYSLVICTVLYILIALVLT
                     GMVPYTKLNLSDPLAFVFNEVHQKWIGYFISVSAVVATTSVLLVFQLGQPRIWMSMSR
                     DGLLPKAFGKIHPKFQTPWFSTIVTGLIVGIPTLFMGSGLMTQLTSIGTLFAFVLVSG
                     GVLLLPRVSSARRGFRLPFWNGRYVMPFLLAGFVWLFRDRIGTDVRNLSSDGLEEVLF
                     LVFVLLVFSLSIASILRKLSLIPVLGVLFCAYLLIEIPAIAWEWFFAWMAIGLTIYFI
                     YGYRKSRLTGNRLPSAAGAFPKFESAE"
BASE COUNT          701 a          736 c          773 g          598 t
ORIGIN      
        1 acagtggtta gtttcccgtt cgtccgcctg gagatctcga ccgcctggcg gcaacctttt
       61 aaaaagatct cgacgttttc cggcttcccg gtagcggtgg aattggcgcc gttgccgcct
      121 ttgtccagaa cgaaaacgcc catcgacatc cccagtttgg caagcgtatc gccgatctgt
      181 tgctgctgct ccggagggcg ctgggccatt ccattgtctt caatcgagcg aaaaccctga
      241 tcgtaagcaa atttgatctg gtctatgaag tcgggcccgg cgctgttcct gaacatcccg
      301 tcatgggttg cgtagttcag gtgaaaaggt tctcccgcgg cggatttccc cggggttccg
      361 gaagagacaa gccgtgaggc agcatttgcg gcgccggcag caacgacaga tgcaccagcc
      421 aggaaggacc gtttgaggaa ggatttgcgt ttcatcctgc aatgttaagc caatctttac
      481 cgtattattt cccgtattga aagaaaattt cggtcgcacc gtaaccatac cgggggtcgt
      541 accggttgat aaaggacttg acctggcgct ttgtccgcag gatctcgtgg atctcatccc
      601 gcagtttgcc tgtccccacc ccgtggatca cgatcatcga aggaagatgg tgcgcgatcg
      661 cgaggtcgaa gtatttttcg aaagtctgca attggagggt aaggatctcg aaattgtcca
      721 gggcgtcggg gttgtcggtc agccgcccgg catgcaggtc cagttcggag cgggcgggct
      781 cgagatgctg ccgcgccctc gaagcgtcgt aaatccgaaa tccagattcg ccaagcagac
      841 ggcgggcatc aacgggctcc tccaccgggc ggaacggata ttcgtcaaag agcttgaacg
      901 agaaggtggc ctcccctttc ttcctgatct ctccgatccg ggcgaacacc tgtttcgccc
      961 gtggtttgaa ggtcgtttca taatattcaa ccctggcctt atcgggagcc agcagggaaa
     1021 attcccattc aagcgacggg ttatcgttaa ggttgggaaa gggaatgtcg tgcaggtaaa
     1081 aatcgtggcc ggcaaggatt tgattggcca gatcgaagtc ggtcttacca aaatagttca
     1141 accggtacga aaaatggtag cccaaagcac tgttgtttac caggtagacc ttcaggctct
     1201 ccacgacatc gtcgtcgaac tcgtcgcggt caaataccgg cagaaaggtg atccaaacgc
     1261 cggtagccag ctgggcaatc cgctcctttt tcttcccggg ctgcggcttc tcgggaggga
     1321 gctggtcgac atattttttc aatcgctttt cggcggggaa cagcttcttc tccgtgaatt
     1381 gcttgaaata aggaaagtca agctgatccg tataggcggg aaacctgacg ccccggacgt
     1441 tcacaacgac catcttgtcg ttaatgatct ccactacctc gccgtcgtca ttggaatgct
     1501 tgactacaac cttgtcgcca acctcgaact tcataatgca aaattacatt attccgcaga
     1561 ctcaaatttg gggaaagccc cggcagcgga tggtaaccgg ttgccggtaa ggcggctctt
     1621 gcgatagccg taaataaaat aaatggtgag tccaatggcc atccaggcaa aaaaccattc
     1681 ccaggcgatg gccgggatct cgatgagaag ataggcgcag aagagtacgc cgaggacggg
     1741 aatgagggat aacttacgca ggatggaagc gatcgaaagg ctgaagacaa gcagaacaaa
     1801 aacaagaaaa agcacctcct caaggccatc gctgctcaag ttccggacgt cagtgccaat
     1861 gcgatccctg aagagccaga caaatccggc aaggagaaaa ggcatgacgt agcggccgtt
     1921 ccaaaacgga agccggaaac cgcgccgggc gctgctgacc cgcggaagca acaaaacccc
     1981 gcccgacacc agtacaaatg cgaacagtgt accgatgctt gtcaactggg tcattagccc
     2041 gctgcccatg aacaacgtcg gaatgcctac tatcagaccc gtaacgatcg tggaaaacca
     2101 gggagtctgg aatttcggat ggatcttgcc aaatgctttt ggcaggagac cgtcacgcga
     2161 catgctcatc cagatccggg gctggcccaa ctgaaatacg agtaaaacgc tggttgtcgc
     2221 cacgactgcg ctgaccgaaa tgaaataccc gatccatttt tgatgcactt cgttgaatac
     2281 aaaggccagt ggatccgaca ggttcagttt cgtgtaggga accatccccg taagcacgag
     2341 ggcgatgagg atatacagca cggtgcatat cacaagggag tagatcattc ctttcggcag
     2401 gtcgcgttgc gcattggtgc actcttcggc ggtggtcgag atggcatcaa agccgatata
     2461 cgcataaaaa acggcagaca cgcccttcag cacgcctccg accccatttg gaagaaaggg
     2521 gtgccagttg gcggtattga caaaaaaagc gcccaggata atgacgagca cgactaccga
     2581 caatttaaag atcaccatcg cgttggtcgt ctttttactt tcacggatgc cgatatacgt
     2641 gatataggta atgacgcaaa cgataaaaaa cgccggcaga ttgacgataa aatgcatccc
     2701 ggcgatcgta ggagccgagg tccacgtatg gagggcatcc atcaacggcg ctttcgcttc
     2761 ggccgtaagt ccagcggtct ttgacagggt ttcctgcgcg gcaaggaa
//
LOCUS       DATGKE010000373         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1388702, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000373 DATGKE010000000
VERSION     DATGKE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388702"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VKU83_06420"
     CDS             <1..275
                     /locus_tag="VKU83_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93222.1"
                     /translation="ENVSELKNIVRELEPFGSIEVEDNHSIVSIVGNEITRTPRLLSR
                     LFESLEPVPIRMVSYGGSRHNVSLLIPSAHKAETLRLLNKGLFGLE"
     gene            complement(304..1221)
                     /locus_tag="VKU83_06425"
     CDS             complement(304..1221)
                     /locus_tag="VKU83_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI93223.1"
                     /translation="MKKITLLFLLFPFFMANAQRELPLYGDQPIPNSKPGPDLESVDS
                     SVSPVRYSYSLVSRPTLRVYLPPAGKGNGTAVVICPGGGYLHLAYTHEGTDVARMLNE
                     WGIAAFVLKYRLPNDETMVDKSIGPLQDAQRAIQLVRERASEWGIKPAQVGIMGFSAG
                     GHLASTEGTHFSQPVIANPAGTSLRPDFMILLYPVISFVDSIAHRGSRDNLIGLNPDE
                     KLVIRFSNEYQVTPQTPPTFMVHAEDDKTVPVANSLHFFEALEKNGVPAEMHIYPGGG
                     HGFGLHNPTTTDQWTDRLKNWLASGGWLR"
     gene            complement(1231..2280)
                     /gene="queA"
                     /locus_tag="VKU83_06430"
     CDS             complement(1231..2280)
                     /gene="queA"
                     /locus_tag="VKU83_06430"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLI93224.1"
                     /translation="MKLSQFKFDLPLNLIAQHPAKKREDSRMMVVHRRTGQIENKHFR
                     DILEFFDDKDVFVVNNTKVFPARMYGRKEKTGAKIEVFLLRELNKPNRLWDVIVDPAR
                     KIRVGNKLYFGDNDELVAEVIDNTTSRGRTIRFLWEGNDEEFREMLEILGETPLPKYI
                     KRKPDDEDKERYQTVYAKHEGAVAAPTAGLHFSKELIKRLEIKGVRFAEITLHTGLGT
                     FRPIEVEDLSKHKMDAEYYRIEEDACKIVNKARLGGHRICSIGTTTMRAIESSFTAEK
                     LLKPSEGWTNTFVHPPYQFNIADALVTNFHLPKTSLLIMVCAFAGYDLAMEAYKKAIK
                     DKYRFFSYGDAMLIL"
BASE COUNT          541 a          765 c          685 g          615 t
ORIGIN      
        1 tcgagaacgt ctcggaactg aaaaacatcg tccgggaact ggagccgttc ggcagcatcg
       61 aggtggagga caaccacagc atcgtctcca tcgtcggcaa tgagatcacc cggacgcccc
      121 ggctgctaag ccggcttttt gagtcgctcg aaccggtacc tatccggatg gtcagctacg
      181 gtggcagcag gcataatgta tcgctgctga tcccctccgc tcataaggct gaaacgctcc
      241 ggctgttgaa caagggacta ttcggcctgg aataagggaa gcgtccggcc ccggcaaaat
      301 gccctaacgt aaccatccgc cactcgccag ccaattcttc agccggtccg tccattggtc
      361 cgtcgtcgtc gggttgtgca acccgaaccc atgaccgccc ccgggataaa tatgcatctc
      421 ggccggcacc ccgttctttt ccagcgcctc gaagaagtgc aggctgttgg ccaccggcac
      481 cgtcttatca tcctcggcat ggaccatgaa ggtcggcggg gtctgcgggg tcacctggta
      541 ttcgttggag aaccggatca ccagcttttc gtccgggttc aatccgatga ggttgtcgcg
      601 ggacccacgg tgcgcgatac tgtccacgaa gcttatcacc gggtacagca gtatcatgaa
      661 atccggcctg agcgaagttc ccgccggatt ggcaataacg ggttggctga aatgcgtgcc
      721 ctcggtcgat gccagatgcc cgccggctga aaagcccatt atgccgacct gcgccggctt
      781 gattccccac tctgaagccc gctccctcac cagctggatc gcccgttgcg catcctgcag
      841 cgggccgatc gacttgtcca ccatggtctc gtcgttgggc agccggtatt tcaaaacgaa
      901 ggccgcaatg ccccattcgt tgagcatcct ggcgacgtct gtgccctcat gggtataagc
      961 cagatgaaga tatcctcctc ccgggcagat caccaccgcc gtgccgttcc cctttccggc
     1021 aggcggcaaa taaacacgaa gggtggggcg gctcaccaga ctataggaat aacgtacagg
     1081 gctgaccgat gaatcgaccg actccaggtc cggccccggc ttcgaattgg gaatgggctg
     1141 gtcaccgtaa agcggcaatt cccgctgtgc attggccata aagaacggaa ataataaaaa
     1201 aagcagcgtg atctttttca tctgtagccg ttaaaggatg agcatcgcgt ccccataact
     1261 gaaaaaccgg tatttatcct ttatcgcctt cttatatgcc tccatcgcca ggtcatagcc
     1321 tgcgaaggcg caaaccatga tgagcaggct cgttttcggc aaatgaaaat tcgtgaccag
     1381 cgcgtcggca atattgaatt gatagggcgg gtgcacaaag gtgttcgtcc agccttccga
     1441 cggtttcagc agcttctcgg cggtaaaaga cgattcgatc gccctcatgg tcgtggttcc
     1501 gatcgaacag atgcgatggc cgcccagtct cgccttattg acgatcttgc acgcatcctc
     1561 ctcgatccgg taatactccg cgtccatctt gtgcttgctc aggtcttcga cctcgatcgg
     1621 ccggaaggtg ccgagcccgg tatgaagcgt gatctcggca aaccggacgc ctttgatctc
     1681 gagccgcttg atcagctcct tgctgaaatg cagtcccgcc gtcggggcgg ctaccgcgcc
     1741 ctcgtgtttg gcataaacgg tctgatatcg ctccttgtct tcgtcgtcgg gcttgcgttt
     1801 gatatatttc ggcagcggcg tttcgccaag gatctccagc atttcccgga actcttcgtc
     1861 atttccttcc cataggaacc ggatcgtcct gccccggctc gtcgtattat cgatcacttc
     1921 cgcgaccagt tcgtcattgt cgccgaagta gagtttattg cctacgcgta tctttctggc
     1981 gggatcaacg atgacatccc aaagccggtt gggcttgttc agttcccgta acaggaatac
     2041 ctcgatcttc gcccccgttt tctccttccg gccgtacatg cgcgccggaa ataccttggt
     2101 attgttaacg acgaacacgt ctttgtcatc gaagaattcc aggatgtccc ggaaatgttt
     2161 gttttcgatc tgaccagtcc tgcggtggac caccatcatg cggctgtctt ctcgtttttt
     2221 cgcagggtgc tgggcaataa ggtttaatgg taaatcgaac ttgaactggg aaagcttcat
     2281 ttgaaatgtt aatttttatt gttttagcgg cgcttgcgac aaacggcaca tgataccagg
     2341 tgggatggta aggtggatcg gaaggggggc tccggtgcaa accaggtctt acggcacccg
     2401 atttggcatc atgttacggc atgatttttc aagcgggcaa aggtagcaaa aaaaggccta
     2461 aacccaaaat aatctttcac ttgtcccctc tcgtcaaccc cccccgccgg ctgccgtcaa
     2521 cccccccgcc ggcttccgtc aatcccgccg ccgggaaaat aattttccct ttttccgggc
     2581 ttttatgcaa cttgtatgga ttcgcc
//
LOCUS       DATGKE010000374        12692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1266646, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000374 DATGKE010000000
VERSION     DATGKE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266646"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..671
                     /locus_tag="VKU83_06435"
     CDS             105..671
                     /locus_tag="VKU83_06435"
                     /inference="COORDINATES: protein motif:HMM:NF036988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003972 - RNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5565 family protein"
                     /protein_id="HLI93225.1"
                     /translation="MQKIISLFQRNYDGDRLVRDEVVPGAEWVINGEGIATRKWDGTC
                     CKIENGILYKRYDAKNGKTPPTGFIPAQDPDPVTGHWPGWIEVSATAKEDKWFRQALE
                     GYGDVGAEFEDGTYELCGPRINGNPERLEKHILIRHGHHPIEDLPRTFYHIGSFLSVA
                     GIEGIVWHHPDGRMVKIKAKDFGIKRAR"
     gene            703..954
                     /locus_tag="VKU83_06440"
     CDS             703..954
                     /locus_tag="VKU83_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93226.1"
                     /translation="MKYVNPTVLEPDQVESGKSYYVGELPAGIPFQLGRGRFETVTYP
                     PYKVVSRNCAGGKAEVWNTRAGKLHKVAYRQRVKALTRA"
     gene            1051..1524
                     /locus_tag="VKU83_06445"
     CDS             1051..1524
                     /locus_tag="VKU83_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93227.1"
                     /translation="MEVEKNVPPSYADINRLKKRQQIAFRRFMTAQAPSPQVAQYLDC
                     DRAFLREYMNSKMVKGMNWNNYGEKWVVDHIVPMRLFNLFDPEDLRLVWNYRNLMPLF
                     KEDNLHKEGDLRFSLILLDRFDDGSENFRLLKEKAEMELSSMDKYLPVRKLAKAS"
     gene            1546..2124
                     /locus_tag="VKU83_06450"
     CDS             1546..2124
                     /locus_tag="VKU83_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HLI93228.1"
                     /translation="MEKKQKISKEKGQSICADLLYAGQSRKEILLFITGKYKASDKTV
                     DNWLKAARPIVAERQAAANAIRDRVDKEQTEASAKRLNLSKERLMEELAKVAFQDPRK
                     LYTVDGGLKPIHEWDDEVAGAIGTIESFDVKTDIKEDDQTVDSVTTGTNRKIKAWDKI
                     KAIETLNKMLGYNAPEKKDLNVNLLDGPITFE"
     gene            2126..3499
                     /locus_tag="VKU83_06455"
     CDS             2126..3499
                     /locus_tag="VKU83_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HLI93229.1"
                     /translation="MEIKVKCLPKFRQLYDLPAGTHTVILIGGRGGGKTYEASKFAAF
                     SSTIRRKRIVVVRDEKEAIKESILNEILLRYDTANANGALTGSFDRLDTGIKDSKTGE
                     MVVFTQGFRASSKEKKANLKGVSNVDIALLEEAEDIRDQLKYNTFADSIRKEGALIVI
                     ILNTPDINHWIVKRYFTLEKVDISQIWPTERDDGGYYDIIPKQIPGVVVIKSNFTDNT
                     FLAQTTVESYKAYGDTNSFKFNKHYYLTAILGYASTGRMGQIFSGWRAITNDEFNAIE
                     ARSIFGQDFGSTSPAGLVECKTVNNRVYVRQHNYKGMTEKQIAILYCELGLKDEIIIA
                     DSAEPMTIGRLRNGWQKSELTDQERKDLEEHEKTGKPWRYAMLTKGWNIYPALKPPGS
                     IITGIKKMMDMEIFVTQDSADLWTEYSSYVWATDKNKMPIDEPEDANNHLVDPLRYVI
                     TGQGRYY"
     gene            complement(3496..3804)
                     /locus_tag="VKU83_06460"
     CDS             complement(3496..3804)
                     /locus_tag="VKU83_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93230.1"
                     /translation="MITVGDILICTNNGQLQPDLLACPLILTREYEVIAMKRCRCGVV
                     YVDIGLSLSAEKFDIVCDACARRFNDGIWWFDVKRFKKKPNPRQEVQQANEVLRYIPN
                     "
     gene            3841..4377
                     /locus_tag="VKU83_06465"
     CDS             3841..4377
                     /locus_tag="VKU83_06465"
                     /inference="COORDINATES: protein motif:HMM:NF026080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HLI93231.1"
                     /translation="MKANILLLALSLPVIAGAQKMNPDTLSFNYSGIVQVDSTAAKVL
                     QARARAFVAESFASGKEVVQLDDSEAGILVIKGTIAPIVKVPLLGKIEYGYVHFTAKW
                     QFKDGRYKYALSEFTHEAHEPNMGSGGALTNRKPACGTFTMTEGQWRQIKANTNDRVI
                     EFIDRLRAAMREGNREDF"
     gene            complement(4374..4607)
                     /locus_tag="VKU83_06470"
     CDS             complement(4374..4607)
                     /locus_tag="VKU83_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93232.1"
                     /translation="MSETPITEEYLLANGWESRTFFYAIGGGEDEDDRLIFIKDGLAL
                     TEMGGFWYRADEDELEYRQGFPSQDRVYFIEEL"
     gene            complement(4604..5101)
                     /locus_tag="VKU83_06475"
     CDS             complement(4604..5101)
                     /locus_tag="VKU83_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93233.1"
                     /translation="MSLNLEPINELHRKIQEVLKEKHMIQPNELRKGNKVYGGNPARC
                     IATVLTIIQQGIEAEYQTITYGAAPNNPGIGFGKFEDIYPIPLTPEILGKCGFERNED
                     KDVNGYLAYRSDHFYAFDIFINKDGKVAVHFLKTALSQNHPQYLHQLQNTIFVLTGEE
                     LEVKL"
     gene            complement(5132..5386)
                     /locus_tag="VKU83_06480"
     CDS             complement(5132..5386)
                     /locus_tag="VKU83_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93234.1"
                     /translation="MNPHHLHMDRREWLENICIIPVMFSVFPIPLAMDIVVFSFFFKD
                     VCWPVPTVFFTWLAIGCYLIYLNERDKKPPKWAKKLFWLS"
     gene            complement(5399..6031)
                     /locus_tag="VKU83_06485"
     CDS             complement(5399..6031)
                     /locus_tag="VKU83_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93235.1"
                     /translation="MGKHNLPASGKFGRLTILEEAIPRGIRRERYVSAICECGKIGEY
                     RLQNLKNGNTTSCGCFHRKADGIWDHKIYKVLSSMKGRCYNPKSVGYKYYGARGIVVC
                     DEWLKDKKAFFRWAIANGWAEHLEIDRIDVDGNYEPLNCRFVLPKANMRNRRTTTFIV
                     FNGQKRALTEWAELYNLPQQVVRKRFQSGWDLQKLFSTPVIDNKSSLLRI"
     gene            complement(6064..6696)
                     /locus_tag="VKU83_06490"
     CDS             complement(6064..6696)
                     /locus_tag="VKU83_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLI93236.1"
                     /translation="MKKLIIDGEEYKFDRYGALHQLKPKPYTYDASYCATYDTPEYER
                     RSELLQGLRLAFSTGVHGKPIQSILDVGYGNGAFMKFAKKQVPVVYGHDISKVPVPAG
                     CDFVEDINIVCDVICFWDCLEHYPNIQFVKDLRAETIVLSLPFFPGWDKFPTWPHRKP
                     HEHLHHFTLESMRKWMWKMGWRMVAHSKHEDIVRRRDTDWNILSCGFRRK"
     gene            complement(6677..6925)
                     /locus_tag="VKU83_06495"
     CDS             complement(6677..6925)
                     /locus_tag="VKU83_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93237.1"
                     /translation="MKVLISWHVIETDHIVSISPTSVDHTGWIGFDIHLAHMSKPLRA
                     EHREQAGAASMRDQLIAIWTGHGIIQEIKASNNEKTDH"
     gene            complement(7175..7906)
                     /locus_tag="VKU83_06500"
     CDS             complement(7175..7906)
                     /locus_tag="VKU83_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93238.1"
                     /translation="MGKTAAISGGVGDAIYSIPVMRALGVTRLYIKENFYHPPFGSMH
                     SVLKPLLELQGIECLPTKGGLDFEIYEDGLQFDYDLDTWRHERGRGRNHIMFSMLTHW
                     RKFYREWRRPWLHNIPVSQGDYSLIFLSWRWRDGSKVDWKKIYDSIPRPVYFIGLPED
                     HELFEREAGQIEWKKTMDLLEMAQLIAGCRALYCNQGVALVIAQGLGKEYLCAFKPTK
                     TNCMMRTPNEHDLNQYKQYEKKDQK"
     gene            complement(7899..8363)
                     /locus_tag="VKU83_06505"
     CDS             complement(7899..8363)
                     /locus_tag="VKU83_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HLI93239.1"
                     /translation="MKWHDIPGYQGKYQISEDLQIRSNECIIYQRDINGNFFSRVQPE
                     KILIPFVNSGYFKINLNKQMKYVHRIVATVFVPNPNNYSVVNHVDGNKLNYHPSNLEW
                     TTDQLNCQHAWKTGLTKMTDGMRQGLRVGQLLKGYKSKVRRDEATGKFMANG"
     gene            complement(8369..8908)
                     /locus_tag="VKU83_06510"
     CDS             complement(8369..8908)
                     /locus_tag="VKU83_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93240.1"
                     /translation="MSLPPSYNLLAKYPNRYFCESGSYRGDAIQQALDAGFQQIRSMD
                     IDKENVTFCHNRFDLYRGKYPNIYVRQGDSSKDFYQLFGGVQEPITFWLDAHSQLFED
                     EIEMGCPFPLFEELEQIAAHPVKTHTILIDDILVLTHPDVTGWSRHTLENTLWLINPA
                     YKFEYVANPVRNNLLIATI"
     gene            complement(9096..9332)
                     /locus_tag="VKU83_06515"
     CDS             complement(9096..9332)
                     /locus_tag="VKU83_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93241.1"
                     /translation="MEYSTGGTPMLVEYLQGDAPPRLLNAFYLNKMGFQNAPGNSYVK
                     DGITIKYDGVYWWYYKDAAGFKIETVEELEKLMA"
     gene            complement(9341..10198)
                     /locus_tag="VKU83_06520"
     CDS             complement(9341..10198)
                     /locus_tag="VKU83_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93242.1"
                     /translation="MRTIKTNPGIGDSLWIIQKLIHTGEKFHWQIHDGNPRRGKQVFD
                     LLPQVTASCEYKPGLQYSVIKPNNIARHINIWKAIRQDDFYLSANEHLEAGKRIENLL
                     PDLPTSYELPFQTQAWEAEVKERFTAGPYIGIYASAYSTARNWGFWDEHGWLKLIQLV
                     KGLIPEATFILIGAEWDAELAGNLRNLLQRHHISYISTIGQPLGYVIEMMKVLAYGFY
                     FPSGLGILSGLLHRPSVMFYPPALPRLACTWADPAIIQDGSFKECQFTTPEKIFAWVK
                     DVYKLDKKI"
     gene            complement(10403..11068)
                     /locus_tag="VKU83_06525"
     CDS             complement(10403..11068)
                     /locus_tag="VKU83_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93243.1"
                     /translation="MRYALRGFERFMKPEKVYIIGALPNWIKNVEHIPAKDVPDTAMR
                     ERNIFEKLLLCPEEEFVYCGDDYYLLESWVEEYAWDMPLAQKLYTLGRFSTYQKTVAN
                     TLRIMPQGRNNDTHCPITLKRKILHSLKRINWTQPNGYCLQSLYCNAAGIKGVEYADL
                     KLRQPFTFGDIMDRKWFSTADGVVDKVVPVMDLLYPAASQYEGDVEMSKQKPAKKDKA
                     VKA"
     gene            11508..>12692
                     /locus_tag="VKU83_06530"
     CDS             11508..>12692
                     /locus_tag="VKU83_06530"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HLI93244.1"
                     /translation="MANNGIIKAGQKLVQGLSQKFNGDPYIAEAAVLVPSQFRDNAGN
                     PNWFFGPNGVDYQFQYADLNSASTAYSRCPPLAAVINRKAQAFINGNTVFLNSKGKDV
                     SKTDSMAKRIGTLLSKPNPLHSWKSFEAQNYIYQQLYGFCIVMPIYSFGFESLGPAYA
                     SSLWNIPSYMVSCREVTNKEWYKAQSISDIIPTIRLKYKDLNVEIPTDQLFIFKDFTP
                     SMDSVVFPDSRVKALRMPINNIISSYESRNELINYAGSQGILTPEMDQISMIPLRDEQ
                     KVQLQQDFRRQYGIKRGQSRYIISPAPMKWQPMGKATKDLMLFEEISDDIMRICDGYV
                     YPSPLLNSEKGPSVSNTDSYKKQVYEDAIIPESLSVYEGWNKFFRLEDTNLTIGKTYD
                     HLP"
BASE COUNT         3379 a         3117 c         2953 g         3243 t
ORIGIN      
        1 gcaccaggta tttaaaaagc tgggggctca atcggcaccc catgcggtca atatagcctg
       61 gcagaggggg atatttacga aggaaaatac aaaaatgaca taacatgcaa aagatcatca
      121 gtttgtttca acggaattac gacggcgacc gcctggtaag ggatgaggtt gtcccgggcg
      181 cagagtgggt cattaatggc gaaggcatcg ccacccgcaa atgggacggc acctgctgca
      241 agatagagaa cggcatactg tacaagcgtt atgacgcgaa gaacggcaaa acgccgccga
      301 cgggctttat ccctgcccaa gatcccgatc cagtcacggg ccattggccg ggctggatag
      361 aggtttcggc caccgctaaa gaggataaat ggttccggca agccttggaa ggatatggcg
      421 atgttggggc ggagttcgag gatggaacct atgaactctg tggaccacgg atcaatggca
      481 accccgaaag gctcgaaaag cacatcctta tccgccatgg tcatcatccc atagaggacc
      541 ttccgcgaac cttctaccat atcggttctt tcctttctgt ggccggcata gagggcatcg
      601 tctggcatca cccagacggc cgcatggtca agatcaaggc caaggacttc ggcatcaaac
      661 gagcccgcta aaatcactca taacctatta aaagcaatat caatgaaata cgtaaatcca
      721 accgtcctgg agccagacca ggtagagtcc ggtaaaagtt attatgtagg agaacttccc
      781 gccggcatcc cttttcagct cggcaggggc cggtttgaga cggtaaccta tcctccctat
      841 aaggtggtat cccggaactg cgccggtgga aaggcagaag tctggaacac ccgggcaggc
      901 aagctgcata aggtggccta ccggcagagg gtaaaggcac ttacacgggc gtagtatttt
      961 atctataaca ttcagaaagt tctgaaagtt atgtaactta gatcgacgtt ccattggaaa
     1021 caatttcata attggttaaa agttaatgag atggaagtag aaaaaaatgt tccaccttct
     1081 tatgcagata taaatcgcct caagaaaagg cagcaaatag ccttccgtcg ctttatgacg
     1141 gcccaagccc catccccgca agtggctcaa tatctcgatt gcgaccgcgc atttcttagg
     1201 gagtacatga actcaaaaat ggtcaaagga atgaactgga ataactatgg agagaaatgg
     1261 gtggtcgatc atatcgtccc catgcggtta tttaaccttt ttgacccgga ggacttgcgg
     1321 ctggtctgga attaccgaaa tctaatgcct ctattcaagg aagataatct ccataaggaa
     1381 ggtgatctca gattttcact cattctattg gatcgatttg atgatggttc agagaacttt
     1441 cgcttactga aggagaaagc tgagatggaa ctatcctcca tggataagta tttgcccgtg
     1501 cgaaagttgg caaaagcctc ctgatgttgt aaaagaattg caagaatgga aaaaaaacag
     1561 aagatatcga aagaaaaggg ccaatcaatt tgcgcagatt tactatatgc gggtcaaagt
     1621 cgtaaggaga ttcttctgtt tattaccgga aaatacaaag ccagcgataa aacggtagac
     1681 aactggctaa aagcggcccg tccaatcgtt gcggaacgcc aggcggccgc caatgcgata
     1741 agagataggg ttgacaagga acagaccgag gcctccgcaa aacgccttaa tttaagcaag
     1801 gagcgattga tggaagagtt ggctaaggtc gcattccagg acccccgtaa attatatacg
     1861 gtagacggag gactgaagcc gatccacgaa tgggatgatg aggttgccgg cgctattgga
     1921 actatcgaga gctttgatgt aaagacagac ataaaggagg acgaccaaac ggtggattct
     1981 gtcaccaccg gcacgaatcg taagatcaag gcttgggaca agatcaaagc catcgaaacc
     2041 ctcaataaga tgttaggata caacgctcct gagaaaaagg acctcaatgt aaatctactc
     2101 gacggaccta tcacattcga ataatatgga gatcaaggtc aaatgcctcc ccaaattccg
     2161 ccagctatat gacctcccgg ccggtaccca tacggtgatc ctgatcggcg gccgcggcgg
     2221 cggaaagact tacgaggcca gcaagttcgc cgcgttcagt tctactatcc gccgaaagcg
     2281 tattgtagtc gtccgggatg aaaaggaagc catcaaagaa tccatcctga atgagatcct
     2341 gctgcggtat gatacggcca atgccaatgg cgccctgacg ggtagcttcg accgactgga
     2401 taccgggatc aaggattcga agaccggcga aatggttgtc ttcacccagg ggttccgggc
     2461 cagctccaaa gaaaagaagg ccaacctaaa gggggtgagc aatgtcgata tagccctgct
     2521 ggaagaagca gaggacatac gcgaccagct gaaatacaat actttcgctg actctatccg
     2581 taaggaaggc gccctcatcg ttattatcct gaacacacct gacataaatc actggatcgt
     2641 caaacggtac tttacgctgg agaaggtcga tatttcccaa atatggccta cagaacgaga
     2701 tgacggggga tattacgata tcatacccaa gcaaatccca ggtgtcgtgg tgatcaaatc
     2761 caacttcacg gataatacct ttctggctca aacaacggtc gaaagctata aagcatatgg
     2821 cgatactaat agcttcaaat tcaacaaaca ctattatctc acagccatcc ttggctacgc
     2881 ctccaccggc cgcatgggcc aaatcttctc cggctggagg gccatcacta atgacgaatt
     2941 caatgccatt gaagccaggt ccatcttcgg ccaggacttt ggcagcactt cgccggctgg
     3001 cttggtagaa tgcaagaccg tcaacaaccg ggtctatgtc cgccagcaca attacaaggg
     3061 catgaccgaa aagcaaatag ccatcctata ttgtgagctg gggttgaagg atgaaattat
     3121 catcgctgac agcgcggagc cgatgaccat aggtcgcctg cggaacggct ggcaaaagtc
     3181 ggagctcacc gaccaggaac gaaaagactt ggaggaacat gaaaaaaccg gtaaaccttg
     3241 gcggtatgcc atgctgacga agggctggaa tatttaccct gccctaaagc cacccgggtc
     3301 gatcataact ggcataaaga agatgatgga tatggaaata ttcgtcaccc aggacagcgc
     3361 ggatctatgg acggagtata gcagctatgt gtgggcgaca gataagaata aaatgcccat
     3421 tgacgaacca gaagatgcta ataatcattt agttgaccca ttacgctatg tcatcaccgg
     3481 ccaaggccgc tattattaat tgggtatata ccgcagcacc tcattcgcct gctgtacctc
     3541 ttgccgggga ttgggtttct tcttaaaccg cttgacgtcg aaccaccaaa tgccatcatt
     3601 aaaccgcctg gcgcaggcat cgcagacaat atcgaacttc tccgcggaaa gcgataggcc
     3661 tatgtccaca tagaccactc cgcatcggca gcgcttcata gcaatgacct cgtattcccg
     3721 ggtaagaatg agcgggcagg ccagcagatc cggctgaagc tggccattat tggtgcaaat
     3781 gaggatgtcg ccgacggtga tcattgcaaa tattgctaaa atagcgtatt tttcgcccaa
     3841 atgaaagcaa atatactcct tctggcctta tccttaccag taatcgccgg cgcccagaaa
     3901 atgaacccgg ataccctttc tttcaactat tccggcattg tgcaggttga cagcactgcg
     3961 gccaaggttc tccaggcccg cgcccgggcc tttgtggcgg aatccttcgc ctcaggcaaa
     4021 gaggttgtcc agctggatga ctcggaggca ggtatccttg ttatcaaagg aaccatagcc
     4081 cccattgtaa aggtcccact cctgggaaag atcgaatatg gctatgtcca tttcacagcc
     4141 aaatggcagt tcaaggatgg acgctataag tatgccttgt cggaattcac gcacgaagct
     4201 cacgaaccga atatggggtc tggaggtgcc ttaacgaaca gaaagcctgc ctgcggaacg
     4261 ttcaccatga cggaaggcca atggcgccag atcaaggcga acacgaatga ccgggttatt
     4321 gagtttattg atcggctgcg ggcggcgatg agggagggga acagggagga tttttaaagc
     4381 tcttcaatga aatataccct atcttgagat gggaagcctt gccgatattc taattcatct
     4441 tcatcagccc ggtaccaaaa tcctcccatc tcagttaatg caagaccgtc ctttataaat
     4501 ataaggcggt catcctcatc ttcgccgcct cctatggcat aaaagaaagt gcggctctcc
     4561 cagccatttg ctaacagata ttcttccgtt ataggcgttt cgctcatagc tttacctcca
     4621 actcttcgcc ggttaggacg aagatcgtat tttgtaattg atgcaaatat tgcgggtgat
     4681 tttgcgaaag ggcagttttt aaaaaatgta cagctacctt tccatcttta ttgataaata
     4741 tatcgaaggc atagaaatgg tcgctgcggt aagccaaata gccgttgaca tctttgtcct
     4801 catttctttc aaacccgcac tttcccagga tctcaggcgt gagggggatg gggtaaatgt
     4861 cctcgaactt gccgaaccct atgcctggat tgtttggagc ggcgccatag gttattgtct
     4921 ggtattccgc ctcaatgcct tgctgaataa tcgtcaagac tgtcgcgatg caacgagccg
     4981 gattgccgcc gtaaacctta ttgcccttcc tcaactcatt gggttgtatc atatgttttt
     5041 ctttaagaac ctcctgtatt ttgcgatgca gttcgttgat cggctcaaga ttgagtgaca
     5101 tttgatcaaa tttagcctaa aaagtggtta tttaagaaag ccagaatagc tttttagccc
     5161 atttcggcgg cttcttgtcc cgctcgttca gatagatcag atagcaacca atggccaacc
     5221 aggtaaagaa cactgtcggg accggccagc agacatcctt gaaaaagaag ctgaaaacga
     5281 caatgtccat tgccagcggg attggaaaga cgctgaacat tacagggata atgcagatgt
     5341 tctccagcca ttcccggcga tccatgtgga ggtgatgagg gttcatagct acttcctttt
     5401 aaatccgcaa tagggacgac ttgttatcaa tcactggtgt tgaaaatagc ttttggagat
     5461 cccaaccgga ttgaaatcgt ttcctaacaa cttgctgagg tagattatat aattcagccc
     5521 actccgttaa tgctcgtttt tgtccattga acacgataaa tgtagtagtt cttctgttcc
     5581 tcatgttggc ctttggaagt acaaatctgc aatttaatgg ctcataattg ccatcaacat
     5641 caattcgatc aatttcaaga tgttcagccc atccatttgc tattgcccat cgaaaaaagg
     5701 cctttttgtc cttcagccat tcatcgcaaa caactattcc cctagcacca taatacttat
     5761 acccaactga ttttggatta tagcatctgc ctttcatgga tgaaagaact ttgtagattt
     5821 tatgatccca aattccatct gcctttctat ggaagcaacc gcaacttgtg gtgttcccgt
     5881 tctttaaatt ttgcaaacga tattcgccga ttttgccaca ttcgcaaatt gcgcttacat
     5941 aacgctcgcg tcttatgccg cgtggaatgg cttcttccaa tattgtaaga cgaccgaatt
     6001 ttccagatgc cggcaaattg tgttttccca taacagctac tgatttacgt tagtaaatat
     6061 aatctatttt ctgcgaaacc cgcagctcaa tatattccaa tccgtatccc gccttctcac
     6121 aatatcctcg tgcttcgaat gcgctaccat ccgccagccc attttccaca tccatttacg
     6181 catggattcc agggtaaaat ggtgaaggtg ctcgtggggc ttgcgatgtg gccatgtcgg
     6241 gaacttatcc catccaggaa agaaaggcag gctcaggaca atggtttctg ccctgagatc
     6301 cttcacaaac tggatattgg ggtaatgctc caggcaatcc cagaagcaga ttacatcaca
     6361 cacgatatta atatcctcca cgaaatcgca tccggccggg acgggcactt tggaaatgtc
     6421 atgcccgtag accacgggga cctgcttttt ggcgaacttc ataaaggcac catttccata
     6481 acctacgtcc aggatagact ggataggctt accatgaaca ccggtggaga aagccagcct
     6541 gagaccctgg agcaactcag accgcctttc atattccggg gtgtcatagg tcgcgcagta
     6601 ggaggcgtcg tacgtatagg gtttgggttt tagctggtgg agagcgccat aacggtcaaa
     6661 tttatactct tcaccgtcaa tgatcagttt tttcattatt gcttgctttt atttcttgaa
     6721 taattccatg acccgtccag atggcgatca gttgatcccg catactggcc gctccggcct
     6781 gctctctgtg ttccgctcgt aaaggcttgc tcatatgagc cagatgtata tcaaatccta
     6841 tccagcctgt atgatcaaca gatgtgggtg agatagatac gatgtggtcg gtttctatca
     6901 catgccagga tataagaact ttcatcttct tctatttaat tttctattgg atagcttgtt
     6961 tttacggcga ttcttcggag ccacaagccg cggaactttc atcatgatga ccatggcagt
     7021 cagcatattt tgtgtgaaat catcgcatgc cattccaatc cttagtgttt cggatcgccc
     7081 aacccatcct ttatcatacg ctagaatcat cttacggacg ccatccattc cgatgtcgtc
     7141 ggcaaattcc tgggagaacc gaatcatttt ccgcctactt ttggtccttc ttttcatact
     7201 gtttatattg gtttaagtca tgctcatttg gtgtcctcat catgcagttt gtctttgtcg
     7261 gcttaaaagc acagaggtat tctttaccta atccctgcgc gatgacaagg gccacgccct
     7321 ggttgcagta cagcgcccta catcccgcga tcagctgcgc catttccagc aggtccatgg
     7381 tctttttcca ttcaatctgt cctgcttccc gttcgaacaa ctcatgatcc tccggcagcc
     7441 cgatgaagta gaccggacgg ggtatgctat catagatctt cttccagtcc acctttgacc
     7501 catcccgcca gcgccatgaa aggaagatca ggctataatc cccctggctg accggaatat
     7561 tatgcagcca cggcctccgc cactcccggt aaaacttcct ccaatgcgtc agcatcgaga
     7621 acataatatg gttgcggccc cggcctcgtt cgtgacgcca tgtatccagg tcgtagtcga
     7681 actgcaatcc gtcctcgtaa atttcgaagt cgagaccgcc cttggtcggc aggcattcga
     7741 tgccttgaag ttcaagtaat ggttttaaaa cagaatgcat actgccaaat ggcgggtgat
     7801 aaaaattctc tttaatgtat aggcgggtaa ctcccaaagc cctcatgacc gggatgctgt
     7861 atatggcatc acccacaccg ccgcttatcg ctgctgtctt acccattggc catgaatttt
     7921 cctgttgctt catcgcgcct taccttagat ttatagcctt ttagaagttg ccccactcga
     7981 agtccctgtc gcattccatc tgtcattttg gtgaggccgg ttttccaagc atgctgacaa
     8041 ttgagctgat ccgttgtcca ttctaagttg gacggatgat agtttaattt attcccgtcg
     8101 acgtggttga caacggaata gttattcgga tttggaacga acacggtggc gacaattctg
     8161 tgaacatact tcatttgttt attaaggttg attttgaaat aaccgctatt aacaaatgga
     8221 attaaaatct tttcgggctg aactctacta aaaaagttgc cattaatatc tctctgataa
     8281 attatgcatt cattagatcg aatttgcaaa tcttcgctaa tttgatattt gccttgatag
     8341 cccggaatgt catgccactt cataaaaatt agatggttgc aattaaaagg ttattcctca
     8401 ccggattggc cacatactcg aacttatacg cagggttgat caaccataac gtattctcaa
     8461 gcgtatgtct tgaccagccg gtcacatctg gatgggtaag gaccaagatg tcatctatta
     8521 aaattgtatg tgtctttacc ggatgcgccg caatctgctc taactcctca aataaaggaa
     8581 acggacaacc catttcaatc tcatcctcaa acaattggct gtgtgcatcc agccagaagg
     8641 ttatcggctc ctgcacaccg ccgaatagtt ggtagaaatc ttttgagcta tccccctgcc
     8701 gcacgtagat gttcggatat ttaccgcggt agaggtcgaa ccggttatgg caaaaggtta
     8761 cgttctcttt atcaatgtcc atggaccgga tttgctggaa accggcgtcg agggcctgct
     8821 ggatcgcgtc gcctctgtaa gatccacttt cacaaaaata gcggttgggg tatttggcta
     8881 aaaggttgta tgatgggggt aggctcataa gtaaaagttt atttgcgtta tgttcatgag
     8941 gatattagga cctccgccac agttttcaac caatgatttc caagtatact tccatccgtc
     9001 tttcgtctcg aatgccttta cctcgaatcg ggcatttttc agaaccagcg ttcctgtctg
     9061 tgtatccaaa aagcccgctt gtaataattt ttctttcatg ccatcaattt ttcaagttct
     9121 tccaccgtct caatcttaaa ccccgccgca tccttgtaat accaccagta gacgccatcg
     9181 tatttgatgg tgatgccatc cttcacatag ctattgccgg gcgcattttg gaatcccatt
     9241 ttattaaggt aaaaggcgtt tagcagtcga ggcggcgcgt ctccttggag atattctacc
     9301 agcatgggcg ttcctccggt gctatattcc atgcggcgga tcatattttc ttatccagtt
     9361 tatagacgtc cttcacccaa gcaaatattt tctctggcgt cgtgaattgg cattccttga
     9421 aactaccatc ctgaataatc gccgggtccg cccaggtgca agccagccgg ggcaaagctg
     9481 gcgggtagaa cataaccgat ggcctgtgaa gcaaccccga caatattccc aacccagagg
     9541 ggaaataaaa cccataggcc aggaccttca tcatctcaat gacgtacccc aacggttgcc
     9601 cgatcgttga aatgtaggag atatgatgcc gctggagcag gttccgcaga ttgcccgcca
     9661 actctgcatc ccattccgcg ccgatcagga tgaacgttgc ttcagggatc aggcccttga
     9721 ccaactggat gagcttcaac caaccatgct catcccaaaa accccagttc cttgctgtgg
     9781 aataggcgga cgcatagatg ccgatatatg ggccagcagt aaagcgttcc ttgacttccg
     9841 cctcccaggc ctgcgtctga aaaggtaatt cataggatgt tggcaggtcg gggaggaggt
     9901 tttctattcg tttgcctgcc tccaggtgct cattggcgga caggtaaaaa tcatcctgcc
     9961 gaatggcttt ccagatattg atgtgacggg caatgttgtt gggcttgatt acggaatatt
    10021 ggagtccggg cttgtattcg caggaggccg ttacttgggg cagaagatcg aatacctgct
    10081 ttccccggcg cggattgcca tcatgaattt gccagtgaaa tttctcgccg gtgtggatga
    10141 gcttctggat tatccaaagg ctgtcaccga tgccaggatt agtctttatt gtccgcatct
    10201 tcagtttttg gttgctcttt aaaaatccac ttcccaatta tccatatccc atgcccagcc
    10261 aataccactg gccagaatat tgctaccagg atatgccgta gatcgtagta tgcctttttg
    10321 ccgtcctcat ggtcggagca gattacgaat agggcgatac cagatagcag caggtattgg
    10381 gctatggaga gaatgatcca aatcatgctt taacggcttt atcttttttt gcgggctttt
    10441 gcttggacat ttcaacatcc ccttcatact gcgaagccgc gggatacaat agatccatca
    10501 ccggcacaac cttatccacc accccgtccg ccgtactgaa ccatttgcgg tccatgatgt
    10561 cgccgaatgt gaacggctgc cgcagtttca ggtctgcgta ctccactccc ttaattccgg
    10621 ccgcattaca atacaaactc tgtaggcaat agccatttgg ctgtgtccag ttgatacgct
    10681 tcaaggaatg caggattttg cgtttcaagg tgatcgggca atgggtatca ttgttccgcc
    10741 cttgcggcat gatcctcaat gtattggcta ccgtcttttg gtaggtcgaa aatcggccca
    10801 aagtatagag cttttgcgcc agcggcatat cccaggcata ttcttccacc caggattcga
    10861 gtagataata gtcatcccca caataaacaa attcttcttc cgggcataat agcagctttt
    10921 cgaagatatt ccgctccctc attgctgtat ccgggacgtc tttggctgga atatgctcta
    10981 cgtttttgat ccaattgggt aatgccccga tgatatagac cttttctggc ttcatgaacc
    11041 gctcaaatcc ccgtagggca tagcgcaaat ccaagtaatt attgtctgaa tgttcgtgta
    11101 acgggatggc taataccatg aaaagatcat taaagtgatc aaatgtaggg aaaaaacgaa
    11161 ataatgttca aatatttcag aaatttctga aagtacggaa agaaattatt ttagatttga
    11221 tacgtggaca atccccccaa gcgagataat ggcaagaaac tcattgagaa ccgttgttct
    11281 tgtggacacc tcatgtttaa aagtcgtggc ttgctgggag agatcgaatt gaagtgcgga
    11341 aagtgtggga atatcaatac gataaagatt cagccagaag gtcggccata ccaggaaaga
    11401 atgggattgg cccgtaagtg aacctccgcg ctgcggtaac tcaataaaaa agaactccaa
    11461 agagggtcag cttcatcata cgatgatggc tggccctctt gtcgtttatg gcaaataatg
    11521 gcatcataaa ggcagggcaa aaattagtcc agggactttc ccaaaagttc aatggtgatc
    11581 cttatatcgc ggaagccgct gtcctggtgc catcgcagtt tcgggacaat gccgggaacc
    11641 cgaattggtt ctttggcccc aatggggttg actaccaatt ccagtatgcg gatctgaata
    11701 gcgcatccac cgcctactcc cgctgtcccc cgctggcagc cgtgatcaac cgcaaggctc
    11761 aggcttttat caacgggaat accgtatttc tcaactccaa aggaaaggat gtcagtaaga
    11821 ccgactccat ggccaagcgt atcggaacgt tgctgagtaa acccaacccg ctgcattcct
    11881 ggaaatcctt cgaggctcag aattatatct accagcagct ttatggcttc tgcatcgtga
    11941 tgcccatcta ctcctttggg tttgaatcct tgggaccggc ctatgccagc tcgctatgga
    12001 atatcccttc atacatggtc agctgccggg aggtgacgaa caaggaatgg tacaaggcac
    12061 aaagcatatc ggacattatc ccgaccattc gcctgaagta caaggatttg aatgtcgaga
    12121 taccaacgga tcaactgttc atcttcaagg acttcacgcc cagcatggac agcgtggtct
    12181 tcccggacag cagggtgaag gccctgagga tgccgatcaa caatatcatc tcttcctacg
    12241 aatcccgcaa tgagctcatt aactatgccg gcagccaggg tatcctcacc ccggaaatgg
    12301 accagatcag catgatcccg ctgcgggatg agcaaaaggt ccaattgcaa caggacttcc
    12361 gccggcaata tggtatcaaa cgtgggcaat cccgctatat catctcccct gccccgatga
    12421 agtggcagcc gatggggaag gctaccaaag acctgatgct attcgaagag attagcgatg
    12481 atatcatgcg catttgtgat gggtatgtat atccttctcc gttgctgaat agtgagaaag
    12541 gaccatccgt cagcaatacc gacagttata aaaagcaggt atacgaagat gcgatcatac
    12601 cagaatccct ttcagtatat gaaggctgga ataagttctt ccgactggaa gacaccaacc
    12661 tgaccattgg aaaaacctat gatcacctgc cc
//
LOCUS       DATGKE010000375         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_613023, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000375 DATGKE010000000
VERSION     DATGKE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613023"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VKU83_06535"
     CDS             complement(<1..1157)
                     /locus_tag="VKU83_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI93245.1"
                     /translation="MSPILQPVLRRMACPSLFALLSFQSVQGQNLVPNSSLEDVNICS
                     EFDQPCSPSAWFYLSRKFTTGYFPRYPSATGKRHLQIVAASRETSNRQYWETMLLCPL
                     QAGERYAVSLKVAAPANRTDLSKTCPNLRDIGMWFTNRFIFVQGDSLLQPRSYLSFTN
                     ASATNLKNGWFEIKKEFVAATNATVLIIGNFYHLPNADIMDQRNAVTPTIDILVDDIS
                     VVPEKPGSCPNRQKVRDSLYSIHRRHSVQLVPEPDANDSLPDIAERVIPEPRPKADTP
                     KSAIPVTPAPETDTIIVHNIQFDFDKYLMENPDTLQRYRSLLTRPGVKKIQVVGYTDD
                     AGSAAYNLALSQRRAREVARQISSKFGIPPALIEPEGRGISRKYPAKYLNR"
     gene            1287..>3097
                     /locus_tag="VKU83_06540"
     CDS             1287..>3097
                     /locus_tag="VKU83_06540"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLI93246.1"
                     /translation="MPGFDTAHVKNIVLLGHAGSGKTTLSECMLYEAGLLTRRGAIEE
                     KNTVSDYNELEQQRGNSIFSKLLHTQWRGYKINIIDTPGYDDFAGEVLASLRVADTGV
                     LLLNANFGVEVGADIIWDHTERLGTPIVLAVNKLDQDKADFDRTVAEARDHFGNHVVI
                     VQYPLNVGLGFNTIVDVLQMTQYRFPAEGGRPEKLPIQDSERAKADFLHKELIEAIAG
                     NDETLLEKYFEKGNLDENEMRQGLKKAMINRELFPLFCLSAKRNMGSGRLMGFIDNIC
                     PGPADMPVPVTLSGAPLPCDPGGPACIFLYKVVSENHIGEMSYFKVYSGTIKPGMELV
                     NGTNGVVEKINQLFIVEGGKRIPVNELSAGDIGATIKLKNSHANNTLHAPGRAIALPP
                     IEFPSPLMSVAIESALKGDEEKLAAAIRQVCEEDPTVLLEMSQELKQTIIYCQGELHL
                     SVIKWKLEHFAKNLRATFSRPRIPYRETIRKPVHSSYRHKKQSGGAGQFGEVYMQVEP
                     WFEGMPDPVGLSVRGREEFQLDWGGKLVFLNCIVGGAIEQRFLPSILKGVMDKMHEGP
                     LTGSHVRDVRVIVYDGKMHPVDSNDISFKIAGMMAFK"
BASE COUNT          720 a          789 c          901 g          687 t
ORIGIN      
        1 ctccggttca ggtatttggc cggatacttc cgcgagattc cgcgaccttc cggctcgatg
       61 agggcaggcg ggatcccgaa tttcgacgag atctggcggg caacttcccg cgctcgcctt
      121 tgggaaagcg cgaggttata cgcggctgag ccggcgtcat ccgtgtatcc cactacctgg
      181 atcttcttca ctccgggcct tgtcagcagg ctgcgatagc gctgcagggt atcgggattt
      241 tccatgagat atttatcgaa atcgaactgg atgttatgaa cgatgatcgt gtcggtctcc
      301 ggggccggcg tgacgggaat ggcagatttg ggggtatccg ccttgggacg aggctccggg
      361 atgacccttt ccgcgatgtc cggcaggctg tcgttggcat ccggttcggg gacaagttgc
      421 acggaatgcc gccgatggat cgaatacagg gaatcccgga ccttctgacg gttcggacag
      481 ctgcccggct tctcaggcac cacggaaata tcgtcgacga ggatatcaat cgtgggggta
      541 accgcattgc gttggtccat gatatcggcg tttggcaggt ggtagaagtt gccaatgatc
      601 agcacggttg cgttcgtggc tgcaacgaac tcctttttga tctcaaacca gccgtttttc
      661 aggttcgtcg cggaagcatt cgtgaaggaa agatagctgc ggggttgcaa gagggaatcg
      721 ccctgaacaa aaataaaccg attggtgaac cacattccga tgtcccgaag gttcgggcag
      781 gttttactca ggtccgtcct gttggctggc gccgcgactt ttagactcac ggcatagcgt
      841 tccccggcct gcagcgggca taagagcatg gtttcccagt attggcggtt agaagtttcc
      901 cggctggcgg ctacgatctg cagatggcgc tttcctgtgg cggagggata ccgcggaaag
      961 tagccggtcg tgaatttccg gctaaggtag aaccaggccg aggggctgca aggctggtcg
     1021 aattcagagc aaatattgac atcttcaagc gacgaattgg gtacaagatt ctgtccctga
     1081 acgctttgga atgacaaaag cgcgaagagg gaggggcagg ccatgcgccg gaggacaggt
     1141 tgcagtatcg ggctcataga aataaggtat tgaagttatg aattctgccg aaaaatcggg
     1201 tcggccggcc cggaccaggc tggcagggaa taccccatgc ggaaattgac cgattttttc
     1261 gtaacttaaa cggaaacacg tttgccatgc ctggctttga caccgctcat gtcaaaaata
     1321 ttgttttgct tggtcatgct ggttctggca aaaccaccct ctcggagtgc atgctttacg
     1381 aagcggggct cctcacgcgc cgcggagcca tcgaagaaaa gaacacggtg agtgactata
     1441 atgagttaga acagcagcgg ggcaattcca ttttttccaa actcctgcac acgcaatggc
     1501 ggggctacaa gatcaacatc atcgatacgc cgggatatga cgacttcgca ggggaggtcc
     1561 tcgcgtccct gcgggtggcg gacaccggtg tcttgctgct caacgcgaat ttcggcgtgg
     1621 aagtaggcgc cgatattatt tgggatcata ccgaacgtct cgggactccg atcgtactgg
     1681 ccgttaacaa attggatcag gataaagcgg attttgaccg gaccgtggcc gaggcccggg
     1741 atcacttcgg caaccatgtt gtcatcgtcc aatatccgct taacgtcggc ctcggcttta
     1801 acaccattgt tgatgtcctg cagatgacac agtacaggtt cccggcggaa ggcggccggc
     1861 ccgaaaaact acccattcag gattcggaaa gggcgaaagc cgactttctg cacaaagagt
     1921 tgatcgaagc gatcgccggc aatgacgaga cattgctgga gaaatatttc gaaaaaggca
     1981 atttggatga aaacgaaatg cggcaagggc ttaaaaaggc catgatcaac cgcgaactct
     2041 tcccgctatt ctgtctttca gccaagagga atatgggcag cggccggctc atgggtttca
     2101 tcgataatat ctgcccggga ccggctgaca tgcccgtgcc ggttaccctt tccggggcgc
     2161 cgctcccctg cgatcctggc ggtccggcct gtatcttcct ctataaggtc gtttcggaga
     2221 atcatatcgg ggaaatgtcc tacttcaaag tatattccgg gaccatcaag cccggaatgg
     2281 agctggtaaa cggaacaaat ggagtagtag aaaagatcaa tcaactgttc atagtggaag
     2341 gcggcaagcg gatacctgtc aacgaacttt ccgccggcga tatcggcgcc acgatcaaat
     2401 tgaagaacag ccatgccaac aataccctgc atgcccccgg aagggccatt gcgcttcctc
     2461 ccatcgaatt cccttcccct ctcatgagcg tcgcgataga atctgcgctc aaaggagatg
     2521 aagaaaagct ggccgcggct atccgccagg tttgtgagga agaccctacg gtgcttctcg
     2581 aaatgtcgca ggagctaaaa caaacgatta tctattgcca gggtgagctc catttgtcgg
     2641 tcattaaatg gaagctcgag cattttgcaa aaaacctgcg cgcgaccttc agccggccgc
     2701 gtattcctta ccgggagacg atccgcaaac cggtgcattc gagctaccgg cataaaaaac
     2761 aatcaggagg cgccgggcaa ttcggcgaag tctacatgca ggtggaaccg tggtttgaag
     2821 gcatgcccga tcccgtcggg ctatcggtgc gcggccggga agaatttcag ctggactggg
     2881 gcggcaagct ggttttcctg aattgcatcg taggtggggc tattgaacaa cgctttctgc
     2941 cgtccatcct gaaaggcgtt atggacaaaa tgcatgaagg cccgcttacc ggctctcacg
     3001 tccgggatgt gcgggtgatt gtctacgatg gcaagatgca tcccgtggac tccaacgaca
     3061 tctcctttaa gattgcgggg atgatggctt ttaaaca
//
LOCUS       DATGKE010000376         5349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1132470, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000376 DATGKE010000000
VERSION     DATGKE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132470"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1015
                     /locus_tag="VKU83_06545"
     CDS             194..1015
                     /locus_tag="VKU83_06545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HLI93247.1"
                     /translation="MYKAVFLDMDGTLLRSDHSVSEATQRTIRMLTGGGIPVILVSAR
                     PVNAVLPTSREIGIPAEYPIISLNGSYIVEKDQPIFQAEIDLGTVVEVAEHVRPFQAT
                     IAYYVQREWYAEVNDAWTEHEQKIMDIKLKVMPLPEMVKDWKARNIAPNKMMVMSEPP
                     AILEIQKHLKSIYDGRLNIYPSKATYLEVMDKAGSKSNAVKFVSERMGLAPKEIIAMG
                     DNYNDIGMIRFAGMGVAMGNAPDDIKKNAKYVTDTNNDDGVRKALEKFFKLEPAR"
     gene            complement(1028..1678)
                     /locus_tag="VKU83_06550"
     CDS             complement(1028..1678)
                     /locus_tag="VKU83_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI93248.1"
                     /translation="MVPYTKKMQHILIADDHSIVRAGLKLLINSSIRDVTIDEAEDGE
                     AVIKKMKSAAFDLLVLDINMPHTESFSLTTYLLKEYPGLKIIIFTMNQELFFAKRFLK
                     IGAHGYLNKYSKEPEIKYAIQKVLSGKRYISDNLAEILSGELNRKKKDNPFEDLSDRE
                     FEVVLQMLKGSSVSEIANTLSLHKSTVGTHKTRILDKLKISNTVELINLARLYEII"
     gene            complement(1724..2368)
                     /locus_tag="VKU83_06555"
     CDS             complement(1724..2368)
                     /locus_tag="VKU83_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93249.1"
                     /translation="MELDKLQQQWKGFPEVSMEERPVLSSDLEKMVVKNPLSDAFYLR
                     RKLLARIIGFIVLSMGDAWILRSQWMGERNDIYLYAAFFLLLLYSLYFHIRLLIFADY
                     PTLLGLPLIPFLAKLEIILDKYVYSFKLMSAITAFSLLILFEQWMARANPAVFEAFLA
                     RDWSKWLLLAFLTISFDILLMHMVIPGYRRLLETVRRYRQSIMAKARKEVWTIK"
     gene            complement(2359..2829)
                     /locus_tag="VKU83_06560"
     CDS             complement(2359..2829)
                     /locus_tag="VKU83_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93250.1"
                     /translation="MHNPTQSCADTDLRSLLKQQHKTVYSICRLFTRTYKEHQRLFTN
                     VISAASQNIRFRKGSASKQTMLWRACVNMAALHSMTTEPDADMKFKSPDYQRSMIELA
                     NTVGDASDYEKFLLFLNLENIASEDLFGSINRPAPKASWSSQFLPFLKEKLIWS"
     gene            3148..4092
                     /locus_tag="VKU83_06565"
     CDS             3148..4092
                     /locus_tag="VKU83_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI93251.1"
                     /translation="MAKDKSNIVSDSVLYLQGKAGKGFVTVAGIAGIEVLGLNVSALT
                     EDLVLNFPPCRGESTYILSFEEVDRSGKADQEGPAADADIVSAAPESTVAITDLVEGK
                     VITYPRDTVIRALIVRIRRSGLEHLIEDFDAGTIESILATSGNDHSLSWPITFKYRLL
                     LEDFMAEIVQHPFRELFTIRNISILVEYLLQQFFVMNKLGRDGNVLSPQDTLSLSRVE
                     EYLCIHYKTEFPGIEKLSRISAMSPTSLKTKFKRYYGETLFGYYQKNKLEHARKLLDS
                     KVPVKVVATEIGYSNPSHFTLAFKKEYGFSPWHYLNPH"
     gene            4135..5052
                     /locus_tag="VKU83_06570"
     CDS             4135..5052
                     /locus_tag="VKU83_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLI93252.1"
                     /translation="MKVQDPAPLKTFPVLNWHSVQLPIGLRVAVVRESASHLDLVLPE
                     TGEKGMSLTFVQFKGELLVRPVTRQDLPAYPASPGMGLLSVSARPLEFKSAGPIQLSA
                     LSISIPEPWIKLFLGDKEQIRKMEELLGPDENIIASRLGRRQLSLFTSALADAQGGKT
                     DLLRLQNSVMGLLEFFLCYRHRSLLGTATSSDIENEDDLILIREVESYISEYYCSDTF
                     TVDSILKKTYTSYHRLNFLFKSIHGMTISEYIRNKRIEKSREMIADNVKSISQIAYEI
                     GYSSISNYILAFKKVYQITPGKYKKQIGA"
     gene            complement(5049..>5349)
                     /locus_tag="VKU83_06575"
     CDS             complement(5049..>5349)
                     /locus_tag="VKU83_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI93253.1"
                     /translation="RNVVDNANKYTTRGSIRILAASQGDKVLITVADTGKGMSPAQVS
                     AFLGPDSLDDLSAGSQLGHKFITDLTRRLDGILSVKSEEGKGTTVTVLLPGSPPG"
BASE COUNT         1326 a         1333 c         1423 g         1267 t
ORIGIN      
        1 gtggcggact gggccagcca ggcggtactg aacgtcgtcc ccggcgcctg gggttcgctc
       61 ttgttctccg ttatttatat gttgatctgc tggagcgtgg gcaagttcct cgacatgaaa
      121 aagatctaca tccgcgtgta acttcttggc ggggggaggg caatggatta aattgcggcg
      181 caaaaaagtt ggcatgtata aagcggtatt cctcgacatg gacggcacgc tgctccggag
      241 cgatcattct gtaagcgaag ctactcagcg gacgataagg atgctgacag gcggcggcat
      301 cccggtcatt ctcgtgtccg cgcggccggt aaatgcggtc ctgcccacat cccgcgagat
      361 cggcatcccg gcggaatacc ccatcatttc ccttaacggc agctatattg tcgagaagga
      421 tcaacccata ttccaggcag agatcgatct tgggaccgtt gtggaggtcg cggagcatgt
      481 caggcctttc caggcgacga tcgcatatta tgtccagcgg gaatggtacg ccgaagtcaa
      541 cgacgcctgg acggaacacg agcaaaagat catggatatc aaactaaagg tcatgcccct
      601 gccagagatg gtaaaggact ggaaggcgcg aaacatcgcc ccaaacaaaa tgatggtgat
      661 gagcgagccg ccggcgatcc tcgagatcca aaagcacctg aagtccatat acgacggacg
      721 gttgaatatc tatccttcga aggctactta cctggaagtg atggacaagg cgggttccaa
      781 atcaaatgcg gtaaaattcg tcagcgaaag gatgggattg gccccgaagg agatcattgc
      841 aatgggcgat aactacaatg atatcgggat gatccggttc gccggaatgg gcgtagccat
      901 gggcaatgct ccggacgata tcaaaaaaaa cgccaaatat gtaaccgaca cgaataacga
      961 cgacggggtg cgcaaggccc tggagaaatt tttcaagctc gaaccggccc gataacaagc
     1021 cgcccagtta tatgatctcg tacaggcggg ccaggttgat cagttccacg gtattcgaga
     1081 tcttcaattt gtcgaggatt cgcgtcttgt gggttcccac agtcgatttg tgaaggctta
     1141 gggtattggc gatttccgaa acggagcttc ctttcagcat ttgcagcact acctcgaatt
     1201 cgcggtcgga gaggtcttcg aaagggttgt ccttcttttt tcggttcagc tcgcccgaaa
     1261 ggatctcggc gaggttgtcg ctgatatagc gcttaccgct caacactttc tggatcgcgt
     1321 atttgatttc cggttccttt gaatacttgt tcaggtagcc atgcgcgccg atcttcagga
     1381 accttttggc gaaaaagagc tcctggttca ttgtgaagat gatgatcttc aggcctgggt
     1441 attctttcag cagataggtc gtgaggctga aggattccgt atggggcatg ttaatgtcga
     1501 ggacgagaag gtcaaaagct gcggacttca tcttcttgat taccgcttct ccgtcttcgg
     1561 cctcgtctat ggtaacatcc cggatggaac tattgatcag gagcttcaac cccgctctga
     1621 cgatcgaatg gtcgtctgcg atgagaatat gctgcatttt cttggtatag ggaaccaaat
     1681 ataaacgata ttaattatat cggaaatgca ggccccgatc tttttattta atcgtccaca
     1741 cctcctttct ggctttcgcc atgatgcttt gacgatacct ccggactgtt tccagcagtc
     1801 tccggtaacc ggggatgacc atatgcatga gaaggatgtc aaacgagatg gtcagaaagg
     1861 cgagcagcag ccattttgac cagtcacgcg ccaggaaggc ttcgaatact gcgggattgg
     1921 cgcgtgccat ccattgttca aagaggatca gcaggctgaa tgcagtgatc gcagacataa
     1981 gtttgaacga atagacgtac ttatcgagaa tgatctccag ctttgcaaga aaagggatca
     2041 gcggcaggcc gagaagggtc gggtaatccg caaagatcag cagccggatg tggaaataca
     2101 gggaataaag cagcagcaag aaaaaggccg cgtacaaata gatatcgttc cgctcgccca
     2161 tccattggga cctgaggatc caggcgtcac ccatcgagag aacgataaaa ccgatgatcc
     2221 tggcaaggag cttccgacgg agataaaacg cgtcggacag tggatttttg accaccattt
     2281 tctcaaggtc agaggaaagt acaggccgct cctccatgga aacttccggg aagcccttcc
     2341 attgctgctg cagtttgtct agctccatat caacttttcc tttaggaagg gcaggaactg
     2401 ggacgaccag cttgctttgg gtgccggccg gttgatagaa ccaaacagat cctcggaggc
     2461 gatattttcc aaattaagga agagcaggaa tttttcgtag tccgacgcat ccccgacggt
     2521 gttcgcgagc tcgatcatag atcgctgata gtcaggggac ttaaatttca tatcagcgtc
     2581 tggctcggtg gtcatcgaat gaagtgctgc catattgacg caggctcgcc aaagcatggt
     2641 ttgtttgctg gccgaacctt tccgaaacct gatattctgg gaggcggcgg aaatgacatt
     2701 tgtgaacagc ctttgatgtt ccttgtaagt ccgggtaaaa aggcggcaga tggagtaaac
     2761 ggtcttgtgt tgttgtttaa gcagcgagcg caggtcagtg tctgcgcagc tttgggttgg
     2821 gttgtgcatt tgggttcgga tttttaagca taggaaaacg gataacgctg taaatttttc
     2881 aataaatgga cagaatagat aaccacaggt tgaatttaat caccgagcca ggacgggcgg
     2941 ccaattgaaa ataagggttt ttgtcgatta tgtatagaac aaaaactatc accaattcat
     3001 ttttttacta caaaaatccg aacccctaat ttctaacttg tcgcggcgtc aaaagcaaag
     3061 gcgcataaaa agccgaataa atttaacccc ataatgagct atgtatctct tgaaaattca
     3121 gaatcttttc tgaaatgttt tgaggtgatg gcaaaggaca aatccaatat cgtatccgac
     3181 tcagtattgt atctccaggg aaaggccggt aagggcttcg tcaccgtggc aggcatcgcg
     3241 gggatagagg tacttggcct gaacgtttcc gccctgacgg aggacctggt cctgaatttc
     3301 ccgccttgcc gcggcgagtc cacctatatc ctgagtttcg aagaggtcga tcgctccggg
     3361 aaggcggacc aggaagggcc ggcggccgat gcggacattg tttcggcggc tcccgaatca
     3421 acggttgcca tcaccgacct ggtcgaagga aaagtgatca cttatccccg ggacactgtc
     3481 atccgcgcgc tgatcgtccg gatccggaga tcggggctgg agcacctcat tgaggatttc
     3541 gacgcaggga cgatagagtc cattctggct accagcggaa acgaccattc gctcagctgg
     3601 ccgatcacct ttaaataccg gcttcttctt gaggacttta tggcagagat cgtgcagcat
     3661 ccgttccggg agttgtttac gatccgaaac atctccatac tggtcgaata cctgcttcag
     3721 cagttttttg tcatgaacaa gctcggccgc gacgggaatg tacttagccc tcaggacacc
     3781 ctcagcctgt cccgggtcga agaatacctt tgcatccact acaagacgga gttcccaggc
     3841 atagaaaagc tttcccggat ctcggcaatg agcccaacca gccttaaaac gaaattcaag
     3901 cggtattacg gcgaaacgct cttcggctat taccagaaga acaagctgga gcatgcgagg
     3961 aagttgctcg acagcaaagt accggttaag gtggtagcga ctgagatcgg ttatagcaat
     4021 ccttcgcatt ttacccttgc ctttaagaag gagtatggct tttctccctg gcattaccta
     4081 aatccccatt gacggcttcc tgctgatacc cttaactcta accgataatt tgctatgaaa
     4141 gtccaagatc ctgctccact caagacgttc ccggtgctaa actggcattc ggtgcaacta
     4201 cccattggcc ttagggtcgc cgttgttcga gagtccgcca gccacctgga cctcgtcctg
     4261 ccggagaccg gtgaaaaggg aatgtccctc acatttgtgc aatttaaagg agaactgctt
     4321 gtccgcccgg tgacccggca ggacctgccc gcttatccgg ccagccccgg catgggtctg
     4381 ttatcggttt cggcaagacc gctggaattc aagtccgccg gcccaataca gctttctgcc
     4441 ttgtccatct ccatccccga gccgtggata aaactatttc tgggtgacaa ggagcagatc
     4501 aggaagatgg aggaattgct tgggcctgac gaaaatatca tcgccagccg gctgggccgg
     4561 cggcagttgt cgctgtttac ttccgcattg gcggatgcgc aaggcggcaa gactgacttg
     4621 ctgcgattgc aaaatagcgt gatgggccta ctggaattct ttctctgcta tcgtcaccgg
     4681 agcctgctgg gaacggctac ttcttcggat atcgaaaacg aggatgacct gatcctcatc
     4741 agggaagttg agtcttatat ctccgagtat tactgttcgg acaccttcac cgtggactcg
     4801 attctgaaga agacctacac cagttaccac cggctgaatt tccttttcaa gtccattcat
     4861 gggatgacga tctccgaata tatccggaac aaacggatcg aaaaatcaag ggaaatgatc
     4921 gctgacaatg taaaatcgat cagccagatc gcctacgaga tcgggtattc cagtatcagt
     4981 aattacatcc tggctttcaa gaaagtttac cagatcactc ccggcaaata caagaaacag
     5041 atcggagcct agccaggcgg gctgcccgga agcaacacgg tcacggtagt tccctttcct
     5101 tcctcgcttt ttaccgacag gatgccatcc aggcgccggg taagatccgt aataaacttg
     5161 tggccaagct gacttccggc gctcagatcg tccaggctgt cggggccaag gaaggcggat
     5221 acctgcgccg gactcatccc cttgccggtg tcggcaaccg tgatcagaac cttgtccccc
     5281 tggctggccg ccaggatccg aatgcttccc cgcgtggtgt acttgttggc attgtcgacg
     5341 acattgcgg
//
LOCUS       DATGKE010000377         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1408145, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000377 DATGKE010000000
VERSION     DATGKE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408145"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="VKU83_06580"
     CDS             complement(<1..1839)
                     /locus_tag="VKU83_06580"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HLI93254.1"
                     /translation="MNYQPSQGLSQFKNLVGIKFQLYNSLFTSLPFHRIEKTGILLSL
                     LLTICEEGYLKKQSPEEILEEFFKKQTALAGERERADILFRFVQYVERQIVLFDALED
                     AAFRHVNDMGGPGTLKHLLSEVVQSNKEEELRAKLKDYSVRMVLTAHPTQFYPGTVLG
                     IINDLSRAVIENNAAEINLYMQQLGKTPFLKKQKPTPYDEAISLIWYLENVFYHASGK
                     IISALRDQFPHVLDNKNALIKMGFWPGGDRDGNPFVKVETTLRVAEALRGAIIKCYYL
                     DVRRLKRRLTFKGVDVLVAGLEVKLYNNIFIPNQRTDLSKEEILGDLAKIREIIIYQH
                     NGLFLNLVDNLIGKVMIFGLHFATLDIRQDSSVHATVLAAVAEREKLLPRDYASLAPR
                     EKIEALTRISGQADPALYPDALIQDTLQSVAAVRTIQQINGMEGCDRYVISHCSSALN
                     VMEVFGLFLLGGWDAKALKVDIVPLFESVEDLGAAASVMEELYTHPVYRRHLAQRGDI
                     QTIMLGFSDGTKDGGYLMANWGIYKAKEELTAVSRKYGIDAVFFDGRGGPPARGGGKT
                     HRFYASMGKNIANREIQLTIQGQTVSSNFGTVDTAQFNIEQLIHAGI"
     gene            2171..>3153
                     /locus_tag="VKU83_06585"
     CDS             2171..>3153
                     /locus_tag="VKU83_06585"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLI93255.1"
                     /translation="MQVLKFGGTSVANAENISKVINLVLESLARDKTIVVVSALGGIT
                     DMLIQSAACAAAGDESYKDTLREIERRHLQTVRNLLPITRQSAALSAVKTRCNEIEDI
                     CSGVFLLGELSPRTQDKIVSYGELLSSCIVAARFASLDQEHAWKDSRELIVTDSRFGH
                     ATVDFGRTNRKIKEYFDSALHALFILPGFIAADEKGVTTTLGRGGSDYTAAILAAATD
                     ARLLEIWTDVSGMMTADPRLVSNTRLIPRISYQEAMELSHFGAKVIYPPTIQPVMRKN
                     IPVWIKNTFSPADPGTVIQLESTHNGDTIRGISSIPRIALLSLEGTGMVGIP"
BASE COUNT          726 a          956 c          753 g          718 t
ORIGIN      
        1 gatacccgca tggattaatt gctcgatatt gaattgcgcg gtgtcgaccg ttccgaaatt
       61 ggagctgacg gtctgacctt gtatggtcag ctggatctcc ctgttggcaa tattcttccc
      121 catcgatgca tagaaacgat gggtcttgcc accgccccgc gcgggcggcc cgccccggcc
      181 gtcaaaaaag acggcgtcga taccgtattt cctggaaacc gctgtcagct cttccttcgc
      241 cttgtagatt ccccaattcg ccatcaggta tcccccgtcc tttgtcccgt ccgaaaaccc
      301 aagcatgatg gtctggatat ctccgcgctg ggccaggtgc cgccggtaga ccggatgcgt
      361 atacaactct tccatcacgg atgccgccgc ccccaggtct tccaccgact cgaacaaggg
      421 gacgatatcg accttcaggg ctttcgcgtc ccaaccaccc aacaggaaca gtccaaaaac
      481 ctccatcacg ttaagcgcgc tgctgcagtg actgatcaca tagcgatcgc agccttccat
      541 tccattgatc tgctggatcg tccggacagc ggctaccgac tgcagcgtat cctgaattaa
      601 ggcatccgga tagagcgccg gatcggcctg gccggaaatc cgggtaagcg cctcgatctt
      661 ttcccgcggc gccagggacg catagtcgcg cggcagcagc ttttcccgct ccgccacagc
      721 cgcaagcacc gtcgcatgca cgctgctatc ctgccggata tccagggtgg cgaaatgaag
      781 cccgaagatc atcaccttcc cgatcaggtt atccacgagg ttcaggaaga gcccattgtg
      841 ctgataaatg atgatctccc ggatctttgc aagatcgccc aatatctctt ccttcgagag
      901 atcggttcgc tggttaggaa tgaaaatatt attgtacagc tttacctcca gcccggcaac
      961 caacacatcc acgcctttga aggtaaggcg ccgcttcaac cggcgtacat ccaggtagta
     1021 acatttgatg atcgctcccc ttaacgcctc tgcgacccgc aaggtcgtct ccacctttac
     1081 aaagggattc ccgtcccggt cgccacccgg ccagaagccc atcttgatca aagcattctt
     1141 attgtccagc acgtgcggga attgatcccg caatgccgaa atgatcttgc cgctcgcatg
     1201 ataaaaaaca ttctccagat accagatcag gctgatcgcc tcgtcatagg gtgtgggctt
     1261 ctgctttttc aaaaacggcg ttttacccag ctgctgcatg tataaattga tctccgccgc
     1321 attgttctcg atcactgccc gggacagatc gttgatgatt cccaggacag tacccggata
     1381 aaattgcgtg ggatgcgctg taagcaccat cctgaccgaa taatccttca actttgccct
     1441 cagctcctcc tccttattcg attgcacgac ctcggacaac aaatgcttca gcgtgcctgg
     1501 accgcccatg tcgttgacat gacggaaggc ggcgtcttca agcgcatcga agagcacaat
     1561 ctggcgttcg acatactgca cgaaccggaa caggatatct gcccgctccc gttcgccggc
     1621 cagcgccgtt tgctttttga aaaattcttc caggatctcc tcagggctct gcttcttcaa
     1681 ataaccttcc tcgcaaatag tcaggagcaa tgatagcaaa atgcccgttt tctcgatccg
     1741 gtgaaaaggc agtgaagtga acaggctgtt atagagctga aactttatcc cgactaggtt
     1801 tttgaattgt gacaacccct gggacggttg ataattcata tcgtattttt aaatgcggtt
     1861 caaagaatgg gcgtcggctg cagaatacat tcaatggagg gccaaattta cttaattccc
     1921 gctaaagaaa tgcgcatttt tttgtttttt cattcaatta gcctacctta gtgcccgatt
     1981 taagataagt gaacaaaggc taccatatta ttcccgtcgc acaggccacg accgcggttg
     2041 cagcgcaagt tacgaccatt gccttcatta caacccgcta agggttgtca aatttcatat
     2101 tacctccggg ctttatcaag ctgttccccc gacaaacggg cttatccatc tctaaataat
     2161 cgatcacatc atgcaggttc taaaattcgg cggcacgtcc gtggccaacg cagaaaatat
     2221 ctccaaagtc ataaaccttg tcctcgaatc cctcgccagg gacaaaacga tcgtagtcgt
     2281 gtctgcgctc ggcggcatca ccgatatgct cattcaatcg gcagcatgcg ctgccgccgg
     2341 ggacgagtcc tataaggata ccctcaggga gatcgaacgg cgtcacctgc aaacggtgag
     2401 gaacctgctt cccatcaccc gccaaagcgc cgcattgagc gccgtcaaga cgcgttgcaa
     2461 cgagatagaa gacatctgca gcggggtctt cctgctgggc gagctttcac cccgcaccca
     2521 ggataagatc gtcagctatg gcgagctcct ttcatcctgc atcgtggccg cccgatttgc
     2581 ctcactggac caggagcacg cctggaaaga ttcgcgggaa ctgatcgtca ccgattcgcg
     2641 cttcggacac gcgaccgtgg attttggccg gaccaaccga aaaataaaag aatacttcga
     2701 cagcgccctc cacgcgctat tcatcctgcc tggattcatc gccgccgacg aaaaaggggt
     2761 taccaccacc ctgggccgcg gcgggtccga ttatacggct gcaatccttg ccgcggcgac
     2821 ggacgcccgc ctgctggaga tctggaccga tgtttccggg atgatgaccg cggatccccg
     2881 gctcgtatcc aacacccgcc tcatcccgcg catttcctac caggaagcta tggaactttc
     2941 ccattttggc gcaaaggtca tctatccgcc gaccatccag cccgtaatgc ggaagaatat
     3001 cccggtctgg ataaaaaaca ctttttcccc tgctgacccc ggcaccgtga tccaactgga
     3061 atcgacgcat aacggcgata cgatccgcgg catttcaagc atcccccgga tcgcccttct
     3121 cagccttgaa ggcaccggga tggttgggat ccc
//
LOCUS       DATGKE010000378         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_210760, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000378 DATGKE010000000
VERSION     DATGKE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210760"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VKU83_06590"
     CDS             complement(<1..580)
                     /locus_tag="VKU83_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93256.1"
                     /translation="MKKPVAVADLLHQLEAEGTEKVKLAVTDIDGVLRGKVISFDKFK
                     SIIEKGFGFCDVVFGWDAADHAYDNGAFTGWHTGYPDALAVVDPNTFRRVPWEGGLPF
                     FLADFSEKGGEVLPVCPRGVLKKVIAQAEAEGYQPYFSAEFEWFNFIASSDDPARRSF
                     DSLRPISQGMFGYSILRASQRSEYFQALFDDLK"
     gene            complement(555..1928)
                     /gene="eat"
                     /locus_tag="VKU83_06595"
     CDS             complement(555..1928)
                     /gene="eat"
                     /locus_tag="VKU83_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HLI93257.1"
                     /translation="MTQQHALSRTLGPVLLWGLGVGYVISGMYFGWNLGLPQGGTLGL
                     AIATIAAIILYVTFTFSYAELACAIPKAGGVFDYASRGLNRDLGFIAGTAQLIEFLFA
                     PPAIAAAIGAYFHLFIPGVSAKEIGVAAYILFTALNIYGVRAAAGFELAVTVVAVFEL
                     LLFCGVTFPHFSWKHIGQNAFPHGWTGVWASVPFAIWFFLGLEGLANVAEEAVNPRRN
                     IPFGFGSALATLVVLCVIVYLSSIGVAGWEAIVYPSPGADPSDSPLPLALSRITGTGG
                     VIYHLLITIGLMGLIASFHGIILAAGRATLEFGRVRYLPASLGRVNASRQTPVNALLV
                     NMVIGIIALLSGKTGQIITISTLGAVTLYVFGMITVLALRRKEPLLERPYRVPGYPVT
                     PVVALVMGLACLVALVWLNIILSVLYISILGLAYIWFTLTAGRRLQDGALRHGKNKTY
                     EETGSSR"
     gene            complement(1948..2478)
                     /locus_tag="VKU83_06600"
     CDS             complement(1948..2478)
                     /locus_tag="VKU83_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HLI93258.1"
                     /translation="MKTIYVMIAVFLTGVCLSGCLKDTPYLDVSHSQPIIEFGLSPAN
                     GQFGPFPYAGDTLGGPNIDVDTAVAVVVASPQVLNSTVTTTIKVDPGQVDAYNTANGT
                     SFEVLPDSLFTLSTTATVSPGYRVGRVAVTLNLCKFPVHHQYGLPLAIVSASTGGGEN
                     LVVSGNSGTFMWLFDR"
     gene            complement(2490..>3647)
                     /locus_tag="VKU83_06605"
     CDS             complement(2490..>3647)
                     /locus_tag="VKU83_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLI93259.1"
                     /translation="NFVISNSSPHSILPGAARIMRAMLFQDLVDQFGNIPYTQAAQPA
                     LTITPKYDSATAIYRDLVVQIDSGIAAIQASQSTVDDASDIMFKGSKSLWLAFANTIK
                     LRILLRQVPNGDQAYVGSEINTILQQGSGFLGAGQDALVQPGYTDATSQSQNPFWADY
                     GFLPNGGGPAQNNNFFVANSFMVNFLNSTADPRLGYFYGKNSLGGYGGNFFGSSLTVS
                     GELSPIGPGILQSPSMPALLFSASQSFFMQAEAVQRGLMPGNYQDLFKAGVEESFRYL
                     GVPNAQAAADQFISSSASQFVNIAASTNPLETILYQKWVAECELDGLEAYCDYRRTGF
                     PVINQVSAAAPGQPMPQRILYPETEYTQNTVHVMTENQQPSDLYTKIFWAQ"
BASE COUNT          869 a         1083 c         1002 g          693 t
ORIGIN      
        1 tcttcaggtc atcgaatagc gcctgaaagt actcgctgcg ctgcgacgcc cgcaggatcg
       61 agtagccaaa cattccctgc gatatcggcc tcaggctgtc aaaagaccgc cgcgccggat
      121 cgtcggagga ggcaataaaa ttgaaccatt cgaactccgc ggaaaaatac ggctggtatc
      181 cttccgcttc cgcctgtgcg atcaccttct tcaggacgcc acgcggacaa acgggcagca
      241 cctcgccgcc cttttctgaa aagtcggcca gaaaaaaagg caggcctcct tcccagggca
      301 cacgccggaa cgtattcgga tcaactaccg ccagggcatc aggataaccc gtgtgccatc
      361 ccgtaaatgc cccattgtca taagcgtggt cggcggcatc ccagccaaag actacgtcac
      421 agaaaccaaa acccttttca atgatggact tgaacttgtc gaaggagata acctttcccc
      481 tcagcacgcc gtcaatatcc gtgacggcca gcttgacctt ttcggtccct tccgcttcca
      541 gctgatgcaa cagatcagcg actgctaccg gtttcttcat acgttttgtt tttcccgtgc
      601 ctcagggccc cgtcctgcag acggcgtccg gccgtcaagg taaaccaaat ataggccagc
      661 cccagaatgg aaatatataa gaccgataaa ataatattca gccaaaccag cgcgacaagg
      721 caggccagcc ccatgaccag cgcgaccacc ggcgtaaccg gataaccggg gacgcggtat
      781 ggccgttcca gcaagggctc cttccgcctt agggccagca cggtgatcat gccaaacaca
      841 tataaggtaa cggcgccgag cgtgctgatc gtgatgattt gacccgtctt tcccgagagc
      901 aacgcgataa tcccaatcac catgttaacg agtaaggcat ttaccggcgt ctgccggctg
      961 gcgttcaccc ggccgagcga cgcgggcaga taccggaccc gcccgaattc cagggttgcg
     1021 cggcccgccg cgaggatgat cccgtgaaaa ctggcgatca ggcccatcaa tccgatggtg
     1081 atcagcaaat gatagattac gccgcccgtg ccggtgatcc tcgataaagc caacggtaac
     1141 gggctgtccg acggatccgc ccccggcgag ggataaacga tggcctccca gcctgcgacc
     1201 ccaatggaag aaagataaac gatcacgcag agtacgacga gcgtcgctaa ggcggagcca
     1261 aaaccgaacg gaatattccg gcgcgggttg accgcctcct cggctacgtt ggccagccct
     1321 tccaggccga gaaaaaacca gatcgcaaac ggcaccgacg cccagacgcc cgtccatccg
     1381 tgcgggaaag cattctgccc gatatgcttc cacgaaaaat gcgggaacgt aacgccgcag
     1441 aacagcaaca gctcgaagac cgccaccacc gtcaccgcca gctcgaagcc cgccgccgcg
     1501 cgaacgccgt aaatattcag ggccgtaaac agaatgtacg ccgccacgcc gatctccttc
     1561 gcggaaactc ccgggatgaa caaatgaaaa taggcgccga tcgccgccgc gatcgcgggc
     1621 ggcgcaaaaa ggaattcgat gagctgcgct gtgccggcaa taaaacccag gtcgcggttc
     1681 aggcccctcg acgcatagtc gaacacgccg ccggccttcg ggatggcaca ggccagttcc
     1741 gcatacgaaa aggtaaacgt cacatacaag atgatggcgg cgattgtagc aatcgccagc
     1801 cccagcgttc caccctgggg gagacccagg ttccagccaa aatacatccc cgaaatgaca
     1861 tacccgacac ccaggcccca taatagcact gggccgagcg ttcgggacaa cgcatgttgc
     1921 tgggtcatgc ctaaagatag gcaatcatca gcggtcgaac aaccacataa aagtgccgga
     1981 gttgccgctc accacgaggt tctcgccgcc gccggtcgaa gcgctgacga tggcaagtgg
     2041 cagcccgtat tgatggtgaa ccgggaattt acagagattc agggtgaccg cgacgcgccc
     2101 gacccggtat ccaggcgaca cggtggctgt ggtcgacagc gtgaataagc tgtcgggcaa
     2161 gacttcaaaa ctggtcccgt tcgcggtgtt gtaggcatcg acctggcccg ggtctacttt
     2221 tatggtcgtc gtaaccgtgc tgttgagtac ctgcggtgag gccacgacta ccgcaaccgc
     2281 ggtgtcaacg tcgatattcg gcccgcccag cgtgtcccct gcataaggga aaggcccgaa
     2341 ttggccgttt gccggactta acccgaattc gatgatgggc tgcgaatggg atacgtccag
     2401 gtatggcgta tccttgaggc aaccgctgag acaaacgcct gtcaaaaaca cggcgatcat
     2461 tacatatatg gttttcataa aaaatccatt tattgcgccc agaatatttt ggtatataga
     2521 tcggaaggct gctgattttc agtcatcaca tggacagtgt tctgcgtata ctcggtctcg
     2581 ggataaagga tgcgctgcgg cattggctgc cccggcgctg ccgcggaaac ctggttgatc
     2641 accgggaatc ctgtccgccg gtagtcacag tatgcctcca ggccgtccag ttcgcattcc
     2701 gccacccatt tttgatacaa aatggtttcc aggggattcg tggaagcggc aatattcaca
     2761 aattggctgg ccgagctgga aatgaattgg tcggcagccg cctgggcatt cggcactccc
     2821 aggtatctga acgactcctc aacgcctgcc ttgaagaggt cctgataatt gccgggcatc
     2881 aaccctcgct gcacggcctc cgcctgcata aaaaaggact ggcttgccga gaatagcaac
     2941 gccggcatcg acggactttg gaggatgccc gggcctatgg ggctcaattc cccgctgacg
     3001 gtaaggctgc tcccgaaaaa gttaccgccg tatccgccaa gcgagttctt accatagaaa
     3061 tagcctagcc tcgggtcggc ggtgctattc aggaagttca ccatgaagct gttcgcaacg
     3121 aaaaaattgt tattctgggc cggtccgccg ccgtttggca gaaagccgta gtcagcccag
     3181 aacgggttct ggctctgcga cgtagcatcg gtatacccgg gctgcactag cgcatcctgg
     3241 cccgccccca ggaaaccgct tccctgttgc aggatcgtat tgatctcgga gccgacataa
     3301 gcctggtcac cgttaggaac ctgccgcagc aggatcctaa gcttgatggt gttggcgaat
     3361 gccaaccaca atgatttcga gcccttgaac ataatatcgg aagcgtcgtc caccgtcgat
     3421 tgcgaggcct ggattgcggc gataccggaa tctatttgaa ccaccaggtc cctatagatc
     3481 gcggtcgcgg aatcgtattt cggcgtaatg gtcagcgcgg gttgggcggc ttgcgtataa
     3541 ggaatattgc cgaactggtc gaccaggtcc tggaataaca tggcacgcat gatccgggcg
     3601 gcgccgggca ggatggaatg aggcgagctg ttgctgatta caaaatt
//
LOCUS       DATGKE010000379         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1369062, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000379 DATGKE010000000
VERSION     DATGKE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369062"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VKU83_06610"
     CDS             <1..791
                     /locus_tag="VKU83_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93260.1"
                     /translation="SLGLFVKYGMMTDDKFLEKAVKFHIMEDVGGGKFYTLDEYRTAA
                     ESLQKNKDGKLVILYSSNPVQQDSYIRQAQGKGYVVVKLETIVDAAFINQMEMKWQDV
                     HFTRVDSDIVDNLIDKAESGSSVLNAEEEQTLKDLFGRQIPELHVTVEVKGLSPEAPP
                     VVATRPEFMRRMKDMAAVSGGMGGFYASMPDEVTLTVNGNHPIYQTILKEGDKPRQEK
                     LVHNLSDLALLSQGLLKGNNLTDFIQRSVDLMGGAAKGRIILEA"
     gene            complement(794..>2398)
                     /gene="recJ"
                     /locus_tag="VKU83_06615"
     CDS             complement(794..>2398)
                     /gene="recJ"
                     /locus_tag="VKU83_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HLI93261.1"
                     /translation="SFEEARAFFRPQLSQLHDPWRMKDMSVAVDRVLQAIRQQEKILI
                     YGDYDVDGTTAVACLFQFLLKACAPGRLDFYIPNRYREGYGVSREGIEHARQNGFSLV
                     ITLDCGIKSAGLIAYAATLGIDFIVCDHHLPDADLPPAVAILNPKQPGCPYPYKELCG
                     CGVGFKLITALASSLGFQAEEPFQYLDLVATAIAADIVPVTGENRILAYYGLKKVNEN
                     PNNGIRALIEINGFKGALKMINLVFMIAPRVNAAGRMDDARKVVQLFIEDDPASAHAY
                     AGQLHADNSDRKEADSNMTEEALAILEKDSAHSCRKSTVLYQPHWHKGVVGIVASRLI
                     ERYYRPTVVLTRSGELIAGSARSVNGFNVYEAIHACREHLLGYGGHFAAAGLTLLPEQ
                     VEAFSHKFEETVATTIRPESLFPELVVDAVLPFDNIRPSFYKILEQMQPFGPDNAQPV
                     FISRGVTDTGSRIVKDLHIRFALRQGPVIMDGIGFNLAAKFPLLQSGAPIDIVYTLEE
                     NEWNNQKSLQLKVFDFALSTAGSPTP"
BASE COUNT          531 a          615 c          752 g          500 t
ORIGIN      
        1 aaagcctggg gctgttcgtt aaatatggaa tgatgacgga tgacaagttc ctggaaaagg
       61 cggtgaagtt ccatatcatg gaggacgtcg gcggcgggaa gttttatacg ttggacgagt
      121 accggaccgc tgccgaatca ctgcagaaaa ataaggacgg gaagctggtc attctctatt
      181 cgagcaaccc ggtccagcag gatagctata tccggcaggc acagggtaag ggctatgtcg
      241 tcgtcaagct ggagacgatc gtcgacgcgg cgtttatcaa tcaaatggaa atgaaatggc
      301 aggatgtcca cttcacccgc gtcgattcgg atatcgtcga taacctgatc gataaggcgg
      361 aatcaggaag ttcggtgctg aacgccgaag aggaacagac cttgaaggat ttgtttggac
      421 ggcagatccc ggaactgcac gtgacggtgg aggtgaaggg tctgagtccc gaagcgccgc
      481 cggtggttgc gacgcggccc gagttcatgc gcaggatgaa agatatggcg gcggtcagcg
      541 gcggaatggg cggattctat gcgagcatgc ccgatgaagt taccttgacc gtgaatggga
      601 atcatccgat ctaccaaacg atcctgaagg aaggcgacaa accgcggcag gaaaagctgg
      661 tgcataatct ttccgacctc gccttattgt cgcagggctt gttgaagggg aataacctca
      721 cggatttcat tcagcggagc gtggacctga tgggtggcgc cgcaaaagga aggattatcc
      781 tggaggcttg acattacggc gtcggactgc ccgcggttga aagggcgaaa tcaaacactt
      841 tcagctgcag gcttttttga ttattccatt cgttttcttc aagggtgtag acgatatcga
      901 tgggcgctcc cgactgcaac agcgggaatt tcgccgcaag gttgaagccg atcccgtcca
      961 tgatgaccgg tccctggcgc agcgcgaagc ggatatgcaa atccttaacg atcctggaac
     1021 cggtgtctgt cacgccgcgg ctgatgaaga caggctgggc attgtcgggg ccgaaaggct
     1081 gcatttgttc gaggatcttg taaaaagagg gcctgatatt gtcgaaaggc aggacagcgt
     1141 cgacaacaag ttccggaaag agggattccg gccggatggt ggtggcaacg gtctcctcga
     1201 acttatggct gaaggcttct acctgctcgg gcagaagggt caggcccgca gccgcaaagt
     1261 gcccgccata tcctaacagg tgctcgcggc aggcatggat cgcctcatat acattgaacc
     1321 cgttgacgga ccgggcgctg ccggcgatca gctctccact ccgggtcagg acgacggtgg
     1381 ggcggtaata gcgttcgatc agcctgctcg ccacgatgcc gacgactccc ttatgccagt
     1441 gcggctgata caagacagtg gatttacggc aggaatgggc ggaatccttt tcaaggatgg
     1501 caagcgcttc ctcggtcata ttgctgtccg cttctttccg gtcgctgtta tcggcgtgga
     1561 gctggccggc gtacgcgtgg gcgctggcgg ggtcgtcctc gatgaaaagc tggaccactt
     1621 tgcgggcatc gtccatccgg ccggccgcat taacgcgcgg cgcgatcatg aagacaagat
     1681 tgatcatttt cagcgcgcct ttgaatccgt tgatctcgat cagcgcccgg atgccattgt
     1741 tgggattttc attcactttt tttagtccgt agtaggcgag gatccggttc tcgcccgtca
     1801 cgggcacgat gtccgcggca attgccgtgg ccaccaggtc gagatattga aacggttctt
     1861 cagcctgaaa gccgagggag gaagccagcg ccgtgatgag cttgaacccg acgccgcagc
     1921 cgcacaattc cttatacggg tagggacagc ccggttgctt cggattcagg atggcaacgg
     1981 cgggaggcag gtccgcatcg ggaaggtggt ggtcgcatac gatgaagtcg atgcccagcg
     2041 tggcggcata ggcgatcagg ccggccgatt tgatcccgca gtcaagcgtg atgaccaggg
     2101 aaaagccgtt ttgacgggca tgctcgatcc cctcccgaga gacgccgtac ccttcccggt
     2161 agcggtttgg aatgtaaaag tccagccggc cgggagcgca ggccttaagg agaaattgga
     2221 aaaggcaggc gaccgcggtt gtcccgtcca catcgtaatc gccatagatc aggatctttt
     2281 cctgctgccg gatggcctga aggacccggt ccacggctac gctcatatcc ttcatccgcc
     2341 acggatcatg gagctggcta agctgcgggc ggaaaaatgc ccttgcctct tcgaagct
//
LOCUS       DATGKE010000380         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_834056, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000380 DATGKE010000000
VERSION     DATGKE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834056"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VKU83_06620"
     CDS             complement(<1..1435)
                     /locus_tag="VKU83_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI93262.1"
                     /translation="MLIPTATYRVQLHAHFTLENLSSILNYLYELGISTVYASPLTTA
                     FRGSQHGYDVADPLHLNPEIGTERQWEDLAAKLKDKGMSWLQDIVPNHMAYSTENPWL
                     YDVLERGKSSSYYSYFDIQPEPVDPLGDRLLAPFLGSPLEDCLKKGELTLQYTDKGLV
                     IRYYDYDFPVAAQLYTSVCTEGGKCPEPLRASLEELVIASLSPAEEWKAAKRRWLALM
                     ANEPSFTEYLQRQVAHFSKNAQRLETLLGSQHYMLAHHRLSSSVINYRRFFTVNSLIC
                     LRMETEAVFADYHREIHRWWRRGLIQGLRIDHIDGLAAPRQYIDRLRQWFAKDCYMIA
                     EKILARDEQVPAGWDLEGMTGYEFLWLADQVLTDARGSRELLDYYCREVIQLPDFAAV
                     VTERKHNFLLKQMGGELNNLFGMLNALPRPEGEPLDPERLRAALAMLLACFPVYRLYP
                     DSGPFNEADRHLIGEAFSRAGTNRPDCT"
     gene            complement(1440..3230)
                     /gene="treZ"
                     /locus_tag="VKU83_06625"
     CDS             complement(1440..3230)
                     /gene="treZ"
                     /locus_tag="VKU83_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HLI93263.1"
                     /translation="MNHQFIPVGARYAPDSTTFIVWSPFRSVELLLTGPAPAAHRMER
                     DERGYWRCRAQVAPGSGYLFRLDGKDSFPDPASLQQPTGVHGPSAVVDLSRFRWEDEG
                     WTGIPLANMIIYELHAGVFTPSHDFDGIVTKLDHLADLGVNAIELMPLAQFPGNRNWG
                     YDGAYPFAIQHSYGGADGFRRLVNAAHQKGIAVVVDVVYNHFGPEGNYLAQYGPYFND
                     QYRTPWGSAINFDGAWSDEVRHFFFENARLLLEDFRVDALRLDAVHAMKDFSAIPFVQ
                     QLKELASEIQRRTGRHKLLIAELDLNDPRYIHPPAKGGYGLDGQWVDEFHHALRTLLT
                     GDRKGYYEDFGEMDHLERAFRNSYVYDGVYSAHRHRTFGSHADACPYDQFVVFDQNHD
                     QVGNRACGDRLTANLTKPQLRLAAAAVLLSPYVPLLFMGEEYAERNPFPYFVSFDDPA
                     LIKAVREGRAREFAGFAGGASTIPDPESPETFERAVLSWDIDQPMLDFYKELIRLRKT
                     RPALQGRTRDTMVVHPAIGNVLVLERKILSDQVWIFLHLGAGTAEVENRTGQALRPIF
                     GTEALDGRPVHPGEPVRLAPWSATVFEIQM"
     gene            complement(3306..>4118)
                     /locus_tag="VKU83_06630"
     CDS             complement(3306..>4118)
                     /locus_tag="VKU83_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLI93264.1"
                     /translation="LQSGNNVWLSGMKIGTVKKLSFYGDSKVDVILSIEKQAQPHIRR
                     DAKARVSTDGLVGNKIVVLTGGSDSAPPVADNDRLESEHLPGMQDMLETLQSSNANLL
                     AITGNLKAVSEKLKAGNGTLGELLNDSSFAQQLRGSIDNLRLASAGSARMMSNLDAFA
                     SRLRNSNGLVNELITDSTVFQRLRGAVGKFNEAAASASAFSASLQNAGQGLNDDHSPA
                     GVLLHDEQMADDLQRTFKNLRVSSQELSDDLEAVQHNFLLKGFFRKKRKAAQ"
BASE COUNT          848 a         1211 c         1186 g          873 t
ORIGIN      
        1 ccgtgcagtc cggccggttc gtccccgccc ggctgaaggc ctctccgatc aaatgccggt
       61 cggcctcgtt gaatggtccg ctgtcggggt agagccggta caccgggaag catgcgagca
      121 gcatcgccag cgcggcgcgg aggcgttccg gatccagggg ttccccttcc ggtcgcggga
      181 gcgcgttcaa catcccgaac aggttgttca gttcgccgcc catttgtttc aggagaaaat
      241 tatgttttcg ttctgtcact acggctgcaa agtccggcag ctgaataacc tcacgacaat
      301 aatagtcgag cagttcccgg cttccacggg catccgtgag cacctggtcc gccagccaaa
      361 ggaattcata tcctgtcatg ccctcgaggt cccagccggc gggaacctgt tcatcccggg
      421 cgaggatctt ctccgctatc atgtaacagt ctttggcaaa ccattgccgc aggcggtcaa
      481 tatattgccg gggcgccgcc agcccgtcga tatgatcgat ccgcaaaccc tggatgaggc
      541 cccggcgcca ccagcggtgg atttcccggt gatagtcggc aaaaaccgcc tccgtttcca
      601 tccgcaggca gatcaggctg ttgacggtaa agaaccgccg gtagttgata acggaagagg
      661 ataaccggtg atgcgccagc atgtaatgct ggctgccgag aagcgtctcc aaccgctgtg
      721 cgttcttact gaaatgggcg acttgtcgct gaagatattc cgtgaatgaa ggttcattcg
      781 ccatcagggc aagccagcgg cgtttggcgg ccttccattc ttccgccggc gagagtgacg
      841 cgatcacgag ctcctccagc gaggcccgca agggttccgg gcatttgccg ccttccgtgc
      901 agaccgaggt atagagctgt gccgccacgg ggaaatcgta atcatagtat cggatcacca
      961 gtcccttgtc ggtgtattgc agcgtcagct cgcctttttt caggcaatct tccagggggc
     1021 ttcccaggaa gggcgccagc aggcggtctc ctaaaggatc gacgggttcc ggctggatat
     1081 cgaagtagga atagtacgat gacgatttgc cccgctccag gacgtcatag agccaggggt
     1141 tctcggtcga ataggccatg tgattgggta cgatgtcctg caaccagctc atccctttgt
     1201 cctttagctt cgctgcgagg tcttcccatt ggcgctccgt cccgatctcg ggattcaaat
     1261 gcaggggatc ggccacgtcg tagccatgct ggctgcccct gaaggccgtc gtcagaggtg
     1321 acgcgtagac cgtcgagatg cctaactcgt acaggtaatt caggatggac gagaggttct
     1381 ccaaagtgaa atgggcgtgc agttgcaccc tgtaggttgc tgtcggaatg agcatcggat
     1441 tacatttgta tttcaaaaac cgtcgccgac cagggggcaa ggcgaacggg ttcacctggg
     1501 tggaccggcc ttccgtccaa ggcctcggtt ccgaatatcg gccgtagcgc ctggccggtc
     1561 ctgttttcca cttccgccgt cccggcgccc aagtgcagga atatccatac ctggtcggac
     1621 agaatttttc gttccagtac caacacattc ccgatcgccg ggtgaaccac catcgtgtcc
     1681 cgcgtgcggc cctgaagggc tgggcgtgtc ttccgcagcc ggatcaattc tttgtagaaa
     1741 tccagcatcg gctggtctat atcccatgag aggaccgcac gctcaaacgt ttcgggcgat
     1801 tcaggatccg gaatggtgga agccccgccg gcgaagccgg cgaactccct ggcgcggcct
     1861 tcccgaactg ccttgatcaa cgccggatcg tcaaaactta caaaataagg gaagggattt
     1921 cgctccgcgt actcttcacc catgaatagc agcgggacat agggagacag caatacggcc
     1981 gcggccgcca gccgcagctg cggtttggtc aaattcgcgg tcaggcggtc tccgcaagcg
     2041 cggtttccga cctggtcatg gttctgatca aagaccacaa actgatcata aggacaggcg
     2101 tcggcgtggc tgccgaaagt ccgatggcgg tgagcggaat aaacgccatc atagacatag
     2161 gaattccgga aagccctttc caggtggtcc atttcgccaa aatcttcata atatcccttt
     2221 ctatcgccgg tcagcagtgt cctcaatgca tgatggaatt cgtccaccca ctgaccatcg
     2281 agcccgtagc cgccttttgc cggcggatgg atataccgcg ggtcattcag atccagctcc
     2341 gcgatcagta acttatgacg acccgttcgc cgttgaattt cggaagccaa ttccttcagt
     2401 tgctggacga aagggatggc actgaaatcc ttcatggcat gcacggcatc gaggcgcagg
     2461 gcgtcgaccc ggaagtcttc cagcagcagc ctggcgtttt caaaaaagaa atggcgcacc
     2521 tcatccgacc aggcgccgtc gaaattgatg gcgctgcccc atggcgtccg gtattggtcg
     2581 ttgaaataag gcccgtattg cgcgagataa ttgccctcgg ggccgaaatg attgtaaacg
     2641 acgtccacca ccaccgcgat gcctttttgg tgggccgcat tcaccagccg gcggaagccg
     2701 tcggcaccac cgtaggagtg ttggatcgcg aacgggtacg ccccgtcgta gccccaattg
     2761 cgattgccgg gaaactgcgc cagcggcatc agctcgatgg cgttgacgcc caaatccgcc
     2821 agatgatcaa gttttgtgac gatcccgtca aaatcgtgcg agggggtaaa aacgccggcg
     2881 tgcagttcgt agatgatcat attggcgagg ggtatcccgg tccatccttc gtcttcccag
     2941 cggaaccgcg acaggtctac gacggctgaa ggtccgtgaa cgcccgtggg ttgctgaagc
     3001 gaggcggggt ccgggaagga gtccttgccg tcgagccgga aaaggtaacc gctgccgggc
     3061 gcgacctgcg cccggcagcg ccagtaaccc cgctcatcgc gctccatgcg gtgagcagca
     3121 ggagccgggc cggtcagcag taattcaacc gaccggaaag gagaccaaac aataaacgtt
     3181 gtactatccg gcgcgtaccg cgcgcccacg ggaatgaact ggtgattcac gaattatcca
     3241 gcaggcaaaa caaatgccga ccatccgggc tgacggatcc ctgcctgtga ggaaatggtt
     3301 ccccactact gcgccgcttt tcgctttttc ctgaagaaac cttttaacag gaaattgtgc
     3361 tgtaccgcct ccaggtcatc gctgagttcc tggctgctga cgcggaggtt cttgaaggtc
     3421 cgctgcaggt catcggccat ctgttcatcg tgcaacagga cgccagcggg cgaatgatcg
     3481 tcgttcaggc cttgaccggc attttgcaat gaggcgctga acgctgaagc cgatgccgcg
     3541 gcctcgttga attttcccac ggcgccgcgc agccgctgga agaccgtcga gtcggtgatc
     3601 aattcattca cgagtccgtt ggaattccgc agccgggatg caaacgcatc caggttgctc
     3661 atcatccgcg cggaaccagc cgaagccagc cgcaggttgt cgatcgaacc acggagctgc
     3721 tgggcgaaag aagagtcatt cagcagttca cccagcgtcc cattcccggc cttcagtttt
     3781 tcactgaccg ctttcaggtt accggtgatc gccaggaggt tcgcgttgct cgactgcagc
     3841 gtttcgagca tgtcctgcat tcccggaagg tgttcgcttt cgagacggtc gttatcggcg
     3901 acgggaggcg cactgtcgct gcctccggtt aagacgacga tcttatttcc gaccagcccg
     3961 tcggtgctga cccgggcctt cgcatctctc cggatatgcg gttgtgcctg tttttcaatg
     4021 ctgaggatta catccacctt cgagtctcca tagaacgaaa gttttttcac cgtcccgatc
     4081 ttcatgcccg ataaccagac attattgccc gactgcaa
//
LOCUS       DATGKE010000381         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_896779, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000381 DATGKE010000000
VERSION     DATGKE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896779"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2480)
                     /locus_tag="VKU83_06635"
     CDS             complement(<1..>2480)
                     /locus_tag="VKU83_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93265.1"
                     /translation="LKQDEDTREDRLRLFAAYGYKSDRNIPTEFAIGEGLVGQVAFEK
                     ERIILSNVPGNYIKISSGLGRAKPANLIILPVLFENKVKAVIELASLDIFSETHLDFL
                     SQLTESIGIVLNTIEASTRTEELLEQSQSLAGELKIQQEELRRTNDELQDKALLLVKQ
                     KNEVEAKNKEVEEARRSLEEKAEQLTLTSKYKSEFLANMSHELRTPLNSLLILAQQLY
                     ENAEGNLTDKQIRYAKTIHSCGDDLIQLINDILDLSKIESGFISANISPVRFSEIAAF
                     VETTFKPISEARHLRFTIETDPKLPVNIETDLQRLNQILKNLLSNSFKFTEKGEVKLR
                     IYEANHNFKPGTPTLDAARQVVGFAISDTGIGIPQEKQNIIFEAFQQAEGSTSRKYGG
                     TGLGLSISRGLAELLGGTIELESTPGRGSTFTLFVPIESSGVIPKEAADSLTSAYQQL
                     QLGAGSGEIETLLSSLRVTTDPADPTKMNIVNEMINDTGDDRNNVQQGDKIVLIVEDD
                     LRFGKIIIEKAHEHDLKAIVATNYIEVFDFVNRFMPIAITLDVKLPDTSGWKVLDLLR
                     NDLNYRHIPIHLISGEENRALALKRGARSFLLKPLENEALDELFEDILSFHRKEARRI
                     LVVEDNELESSQIGKMLRSDNIEVTIAANGKAALQENNVKDFDCIILDYTLPDISGIE
                     LMRQVSENKRKLTPVIVYSAKDFDKKELNHLHRTSNTILLKGVNSLEHLLEETVLHLH
                     INHRDLAPEKRRVIENIRLKDDILTGKNVLVVDDDVRNLFALTTVFERYNINVVTAES
                     GKEAIQIINDDRQKIDMVLMDIMMPEMD"
BASE COUNT          467 a          672 c          706 g          635 t
ORIGIN      
        1 atccatttcc ggcatcatga tatccatcag caccatgtct attttctgcc ggtcgtcgtt
       61 gatgatctgg atagcttcct tgccgctctc cgccgtcacg acgttgatat tataccgctc
      121 aaaaacggtc gtcagggcga acaggttccg gacatcgtcg tccacaacca gcacgttctt
      181 acccgtgaga atatcgtctt tcaggcggat attttcgata acccgccgct tttccggcgc
      241 caggtcgcga tggttgatat gcaggtggag cacggtctct tccagcagat gctcgagtga
      301 attgaccccc ttgagcaaga tcgtgtttga cgtgcggtga aggtggttga gctctttctt
      361 atcgaaatcc ttggcggaat agacgataac cggggttagc ttccgcttgt tctcactcac
      421 ctgccgcatc agctcgatcc ccgaaatatc gggcagcgta tagtcgagga tgatacagtc
      481 gaagtccttt acgttgttct cctgcagggc cgccttgccg ttggccgcaa tggtgacctc
      541 gatgttgtcg ctgcgcagca ttttgccgat ctgcgaagat tccagttcgt tgtcttcgac
      601 aacgaggatg cggcgggctt ccttccggtg gaaggaaagg atgtcctcga acagttcgtc
      661 cagggcctcg ttctccagcg gtttgagcag gaaactccgg gccccccgct tcaaagccag
      721 ggcgcggttc tcttcgccgg agatcaggtg gatggggata tgcctgtagt tgaggtcatt
      781 cctcagcagg tccaggacct tccagccgct ggtgtccggc aacttgacgt ccagggtaat
      841 ggcaatcggc ataaaccggt tgacgaaatc gaacacctcg atatagttcg ttgccacaat
      901 ggccttgagg tcatgctcgt gcgccttttc gatgatgatc ttcccgaaac gcaggtcgtc
      961 ttcgacaatg agaacaatct tatcgccttg ctggacgttg ttgcgatcgt cgcccgtatc
     1021 gttgatcatt tcattgacga tattcatttt ggtcgggtca gccgggtcgg tggtgacacg
     1081 cagggagctg agcagggtct cgatctcgcc gctcccggcc ccaagctgaa gttgctggta
     1141 agcgctggtc aggctgtctg cagcctcctt cggaatgacg ccgctgcttt cgatgggcac
     1201 gaagagcgta aacgtactgc cgcgaccggg ggtgctttcc agctcgatgg tgccgccaag
     1261 cagttcggcc aggccccggc tgatcgacag acccaggccg gtaccgccgt atttacggct
     1321 ggtggaacct tcagcctgct gaaaagcttc aaagatgatg ttctgttttt cctgcgggat
     1381 gccgatgccc gtatcgctga tggcaaatcc cactacctgg cgggcagcgt ccaaggtagg
     1441 cgtgcccggc ttaaagttgt ggttggcctc gtagatgcgc agtttcacct cgcccttttc
     1501 cgtaaacttg aatgaattgg acagcaggtt cttcaggatc tggttcaacc gctgcaggtc
     1561 ggtctcgata ttcaccggca gtttcgggtc cgtttcgatc gtaaaacgaa ggtgacgcgc
     1621 ttccgagatc ggcttgaagg tggtttccac gaaggctgca atctcgctga agcgcacggg
     1681 agagatattt gccgaaatga accccgattc gatcttggaa aggtcgagga tgtcgttgat
     1741 cagctggatc aggtcgtccc cgcaggagtg gatcgtcttc gcatagcgga tctgcttgtc
     1801 cgtaaggttg ccttccgcat tttcgtaaag ttgttgcgcc aggatgagca ggctgttcaa
     1861 cggcgtacgg agctcgtggg acatattcgc aaggaattcc gatttgtatt tggacgtgag
     1921 cgtaagctgt tccgcctttt cttcgaggga gcgccgcgcc tcttccactt ccttgttctt
     1981 ggcctccact tcattctttt gcttcaccag gaggagcgcc ttatcctgca attcgtcgtt
     2041 ggtgcgacgc agctcttcct gctggatctt cagctcgccg gccagcgatt gcgactgttc
     2101 gagcaactct tccgtccgcg tactggcttc gatggtgttc agcacgatac cgatggactc
     2161 ggtcagctgg ctgaggaagt ccaggtgggt ctcgctgaag atatccaggg aggcaagctc
     2221 gatgacggcc ttcaccttgt tttcgaacag caccggcagg atgatcaggt tagccggttt
     2281 cgcgcggccc aggccggaac tgatcttaat ataattgccg ggcacattgg agaggatgat
     2341 cctttccttt tcgaaggcca cctggccaac cagcccttcg ccgatggcga attcggtggg
     2401 gatattcctg tccgatttat agccgtatgc cgcaaagagg cgcagccggt cctcgcgggt
     2461 atcttcgtct tgttttaaga
//
LOCUS       DATGKE010000382         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1041269, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000382 DATGKE010000000
VERSION     DATGKE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041269"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..158)
                     /locus_tag="VKU83_06640"
     tRNA            complement(86..158)
                     /locus_tag="VKU83_06640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Thr,seq:cgt)
     gene            complement(223..1125)
                     /locus_tag="VKU83_06645"
     CDS             complement(223..1125)
                     /locus_tag="VKU83_06645"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI93266.1"
                     /translation="MSMENKRIGWIGLGNMGLPMANNLIKAGFPVTVYNRTASKAGPL
                     QAAGAGLAASPADLWQKADIVITMVSDDAALKAIHEGNRGLAAGIESPAPANPSAGER
                     KLLIDMSTVSPATSRELAGVLASKGVDYLDAPVSGSVKPAELGQLVIMVGGKKECYEA
                     ALPIFGKLGKRAFWLGAQGAGNLAKLAINLLLAFHMEGLAEALVFGREKGIRPEALLA
                     VIGETALANGISRGKADNILAGNYEPAFELRHLVKDLRLALSQGMHTPGGMTIHDTYQ
                     QAMNAGWGQKDMAGIFPYLEGKKV"
     gene            complement(1146..1607)
                     /gene="msrB"
                     /locus_tag="VKU83_06650"
     CDS             complement(1146..1607)
                     /gene="msrB"
                     /locus_tag="VKU83_06650"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLI93267.1"
                     /translation="MDAKKNVPNPVYSHTDTAAVNLTDDQWKDILPKKVYEVARQKGT
                     ERPYSSPLEENHEVGTYYCAVCGNPLFRSDTKFESGCGWPSFYEPVSKGSMIYLPDHS
                     HGMERTEVECGRCKSHLGHVFNDGPPPTGLRYCINGVVLDFESGAPSSDKK"
     gene            1802..>2375
                     /locus_tag="VKU83_06655"
     CDS             1802..>2375
                     /locus_tag="VKU83_06655"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLI93268.1"
                     /translation="MFNLILFGPPGSGKGTQSERLIAKYGLKHLSTGDLLRSEIAGQT
                     PLGLAAKSIIDKGQLVPDEVVIEIISAALDNNPNAHGFLFDGFPRTTAQAEALDKLLA
                     QKGTSISIVLALVVKEEELTKRLLNRGLTSGRSDDRDEMIIRSRHAEYEKKTAAVAEY
                     YKQYGKVVYVNGEGDVDEIFQSLCREIDMRD"
BASE COUNT          522 a          693 c          601 g          559 t
ORIGIN      
        1 gatcttctca cggatctcac cgccaagctt gacgatctca gacccgataa gggtctcggc
       61 aagtcgggat aaatgcataa ctgtgagcca actatcggaa tcgaaccgac gaccctttca
      121 ttacgaatga aatgctctac cagctgagct aagttggcat tatcgggaat tcggcgtgca
      181 aaaatacagg ctttgcggct ggctcgcaaa ataattatag gcttatacct tcttcccttc
      241 caggtaggga aagatccccg ccatgtcttt ttgtccccaa cccgcattca tcgcctgctg
      301 ataggtgtcg tggatcgtca tgccgcccgg ggtgtgcatc ccctggctca gcgccagccg
      361 caagtctttc accaggtggc gcaattcaaa ggcgggttca taattgcccg ccaggatgtt
      421 atccgccttg cctctcgata ttccgttcgc aagcgccgtc tcgccgatga ctgccagcaa
      481 ggcttccggc cggatgccct tttccctccc aaagaccagc gcctcggcta atccctccat
      541 gtggaaggcc aacaaaagat tgatcgccaa ctttgccaga tttccagcgc cttgcgctcc
      601 cagccagaag gcccgcttgc ccagcttgcc aaatatcggg agcgccgcct cgtaacactc
      661 cttttttccg ccgaccatga tgaccagctg tccaagttcc gccggcttca cgcttccgga
      721 taccggcgcg tcaaggtagt cgacaccctt cgaggccagc actcctgcaa gctcccttga
      781 agtggcgggg gataccgtgc tcatatcgat cagcaatttt ctttcacccg ccgaagggtt
      841 ggcgggagcg ggcgattcga tccccgcagc cagcccgcgg ttcccttcgt ggatcgcttt
      901 cagtgcggca tcgtcggaaa ccatggtgat cacgatatcc gccttttgcc atagatccgc
      961 cgggctggcc gccagcccgg cgccggccgc ttgcaacgga ccggcttttg atgccgtccg
     1021 gttatatacg gtgaccggga accctgcttt tataaggttg ttcgccatcg gcagacccat
     1081 gttgccaagg cctatccaac cgatccgctt gttttccatg ctcatcagat gtctggcgcg
     1141 aatgattatt ttttgtcgga agagggagcg ccagactcga agtccaggac cactccattg
     1201 atacaatacc tcaggccggt cgggggcggc ccgtcgttga atacatggcc cagatgggat
     1261 ttgcaccgcc cgcattcgac ttccgtgcgc tccatcccat gactgtgatc cggtaagtag
     1321 atcatgctgc ccttgctcac cggctcataa aagctcggcc agccgcaacc gctttcgaat
     1381 ttggtatcgc tcctgaacaa cgggttaccg caaaccgcac agtagtacgt gccgacttca
     1441 tggttctcct ccaacggact gctatagggg cgctccgtcc ctttctgccg ggcgacctcg
     1501 tagactttct ttggcagaat gtccttccat tggtcgtcgg taagatttac ggcggcagta
     1561 tcggtatgcg agtagaccgg gtttgggaca tttttttttg catccataat tgtatagtgt
     1621 gttatgctgg tctccatttc cgggagatgc caatgtaaca aaacttattc gcaggagttc
     1681 aggaacctcc atccgtcaag gtatcgcctt catctccatc gtaaggaaac cggaaaattg
     1741 ttaaagaatt tttaaaccaa tcgctatatt tgttcataca cacgggtaag tagtacgaat
     1801 tatgtttaat ttaattttat ttggtccgcc cggcagcgga aagggcacgc aatctgaaag
     1861 actgatcgca aaatatggac tgaaacatct cagtaccggc gacctgctcc gttccgaaat
     1921 cgccggtcag acacccctcg gcctggctgc aaaaagcatt atcgataaag gacagcttgt
     1981 tcccgacgaa gttgtcatcg aaatcatcag cgccgcactg gacaataacc cgaacgcgca
     2041 tggcttcctg tttgacggtt ttccccgcac gaccgcccag gccgaagccc tggacaaact
     2101 gctcgcgcaa aaaggcactt ccatttccat tgtcctggcg ctggtggtaa aggaggaaga
     2161 gcttacgaaa cggctgctga accgcgggct gacttccggc cgctccgatg accgcgatga
     2221 gatgatcatc cgctcccgtc acgccgaata tgaaaaaaag accgcggccg tggcggaata
     2281 ctataagcaa tacgggaagg tcgtttatgt caacggcgag ggcgatgtcg atgagatctt
     2341 tcagtcactt tgcagggaga tcgatatgcg cgacc
//
LOCUS       DATGKE010000383         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_211372, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000383 DATGKE010000000
VERSION     DATGKE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211372"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VKU83_06660"
     CDS             complement(<1..2018)
                     /locus_tag="VKU83_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93269.1"
                     /translation="MFLNYLKTALRHFRGHKAMTLINVFCMAVGILCTSVIGTFVGRQ
                     YSYNRNLQDAGQQYVLRSAWNDPNTAPASTTVGPLAKTLAAEFPALVRSAFRALDVQA
                     VIGANGVSRRETITLADTSLVSMYGFSLEAGDSRHAFRDRHSAVLSAGLANQLFGTAH
                     AIGRLIRIQTRLNRLESFSVSAVLKDRTDNSILDNDGSVRGLFIPIDQDTLFMETDAA
                     NDWNDVFVNSYVRLRPGVQPKPVSEALRRILALHTSTFIRNNLVVTPEPLESWYLARN
                     NGSMQKMIIGLLLIGGFILAISVINFINLNISLSMYRLREIGLRKIFGGSRRQIAIQH
                     LTEALFLSAAAAIIGILGYDAARSWVGGLLGTNLPPFGQLGLRVAGFYILFFLLLGLS
                     AALYPAFILSATPVIHASRGKMEAPSAGIRLNKFFLTIQLTVAVLLFISALIIGRQLH
                     FAFHRYPGYDMDQVLVISSLPSQQDSAAASGLFYARDQLARVPGVKAASVSSNIPDGR
                     PQGSATLTPKRSTGSRIICPLITADDKFASVFGLSMAEGRFLDPKGGRGQLVLNESAV
                     RALEVTKGLGTEFEEVAGVRLDVTGIVRNFNISSLHDRIGPLAIMNTTDFPGANYISV
                     KLSGGDIRGTLARLRREWHALYPDLPFGFFFMSDRFESMYHSDSRLQQA"
     gene            complement(2129..3076)
                     /locus_tag="VKU83_06665"
     CDS             complement(2129..3076)
                     /locus_tag="VKU83_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLI93270.1"
                     /translation="MNLKPVATFLLLLVIPIASRAQLRWRKVDTSFGPLPPSVHVYRS
                     PDSLDGSPFIAFYVSARLRDRDLVFTAETTKNQRFTPAEFYQRWQRPLIVVNSTFFSM
                     ASGENWSLVVRNGHLVSRNLPSLHGTGADSALYYYPTRAAIGIDRRRRADVAWTFTDS
                     TRIWPYAFEEMPVIAKGRDSIPSIYDLGDVTWKWWKMRTAVGGGPVLVHDGKLDITYR
                     EEQLYPEPGEYEPRTAMGYTRDGRFIILAIQGRAPGIAVGATLRQEALIMLGLGCYEA
                     INLDGGGSSCLLVNGKETIRPSGREGERAVPTVFIIKRK"
     gene            complement(3092..>3415)
                     /locus_tag="VKU83_06670"
     CDS             complement(3092..>3415)
                     /locus_tag="VKU83_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93271.1"
                     /translation="GGMRTKSGIMLGLGERKEEVLQTMRDLRESGVDVLTIGQYLQPS
                     KKHLPVTRFVHPDEFSELKEAGYQMGFDYVESGPLVRSSYHSERHVVPGYGRNEWLRQ
                     KQAAN"
BASE COUNT          800 a          937 c          976 g          702 t
ORIGIN      
        1 gcctgttgca gtcgggaatc cgagtgatac atcgattcga aacgatcgct catgaagaag
       61 aatccgaaag gaaggtcagg atacaacgcg tgccattccc gacggagccg ggcgagggtg
      121 ccccggatat cgccgccgga caatttcacc gaaatataat tagcgccggg gaagtcggtg
      181 gtattcatga ttgcgagcgg accaatccgg tcatgcaagg aggaaatgtt gaaattcctg
      241 acgatgccgg tgacatccag cctcaccccg gctacctctt caaattcggt ccccagccct
      301 tttgtcacct ccagtgcccg aacagccgat tcattcaaca ccagctgccc gcgtccaccc
      361 ttgggatcga ggaatcggcc ttcagccatt gaaagtccaa aaaccgacgc gaatttgtca
      421 tccgcggtaa tcaaaggaca aattatccta ctgccagtcg atcgcttagg cgtcaacgtc
      481 gcggaccctt gcggtctgcc atccgggata ttcgatgaaa ccgaagccgc cttcactccg
      541 ggcacgcgtg ccaattgatc gcgggcatag aataagccgc tggctgctgc ggagtcttgt
      601 tgtgaaggca gggaagaaat gaccagcacc tggtccatat catagcctgg gtaccggtga
      661 aacgcaaagt gtaactgcct gccgataatc agtgccgaaa tgaaaagcaa cactgccacg
      721 gtcagttgta tcgtcaggaa aaacttgttc agccggattc ccgcggaagg ggcttccatt
      781 tttccgcggc tggcatgaat gacgggcgtg gcgctcagga tgaatgcagg atacagggcc
      841 gccgacaatc ccagcaaaag gaagaagaga atataaaagc ctgccacccg taacccgagc
      901 tggccaaaag gaggaagatt ggtgccgagg agcccgccga cccaggaccg ggctgcatca
      961 tagcccagga tcccaatgat cgccgcagcc gcggagagga ataaggcttc cgtcaaatgt
     1021 tgaatggcaa tttgccgcct gctgccgcca aaaatttttc gaagaccgat ctccctgagg
     1081 cggtacatgg aaagactgat attaagattg ataaaattga tcacggaaat ggccagaata
     1141 aagcctccga tcaggaggag tccgatgatc atcttttgca ttgagccgtt gttgcgggcc
     1201 agataccaac tttctaacgg ttcgggcgta acgaccaggt tgttccggat gaacgtggag
     1261 gtgtgtaagg ccaggatccg gcgcaatgcc tccgacactg gtttcggctg aacgccggga
     1321 cgaagcctca cataagaatt cacaaaaaca tcgttccaat cgttggcggc gtctgtttcc
     1381 ataaacaaag tatcctggtc gatcgggata aagagtccac ggacggagcc gtcgttatcc
     1441 aggatggagt tatcggtccg atccttcagc acggccgaga ccgaaaagga ttcaagcctg
     1501 ttcagcctgg tctggatacg gatcagacgg ccgattgcat gggcggtgcc gaaaagttga
     1561 ttggcaaggc cggcgctgag taccgcggaa tgccggtccc tgaaagcatg ccggctgtct
     1621 ccggcttcca gtgagaatcc atacatggaa accaggctgg tgtccgccag cgttatggtt
     1681 tcccttcgag agacaccatt tgcgccaatg accgcctgga cgtcaagcgc gcgaaatgcc
     1741 gatcggacca gcgcaggaaa ctcagctgca agcgtttttg ccagcgggcc gaccgtagta
     1801 gatgcaggcg cggtattggg gtcattccat gcgctgcgca gcacgtattg ctgacccgca
     1861 tcctgcaggt tcctgttgta ggaatattgc cgcccaacaa aggtcccgat gacagatgtg
     1921 caaaggatgc ctaccgccat gcagaaaacg ttaatgagcg tcattgcctt gtggcccctg
     1981 aagtgccgca gtgccgtctt gagataattc aggaacatgc ccggaccaag tcaaaatttc
     2041 agcccaattt aatactttcc tgccggtaaa gcgagcagga gaacagagtg accggagaca
     2101 gttacgaaac gataccctga ctggccgttt atttcctctt aatgataaag acggtcggaa
     2161 cggctcgttc accttcccgg ccggagggcc ggatggtttc cttgccattg acgaggaggc
     2221 agctgcttcc gccgccgtca agattgatgg cttcataaca acccagtccc agcatgatca
     2281 gcgcttcctg gcggagcgtg gcgccgaccg cgatgccggg cgcccggccc tggatcgcca
     2341 ggataatgaa gcgcccatcc cgggtatatc ccatcgcggt acgtggctca tattctcccg
     2401 gttcaggata caactgttct tcccggtagg tgatatccag ttttccgtcg tggaccagga
     2461 caggtccgcc gcctactgcg gtgcgcattt tccaccactt ccaggtgacg tcccccaggt
     2521 catagatgga aggaatggag tccctgcctt ttgcgattac gggcatctcc tcgaacgcgt
     2581 atggccagat ccgggtagaa tctgtaaaag tccaggcaac gtccgcgcgc ctgcggcggt
     2641 cgatgccgat ggccgcacgg gtcggataat agtaaagggc ggagtcggcg cctgttccgt
     2701 ggagcgacgg caggttccgc gagacaagat ggccgttccg gacgacgagg ctccaatttt
     2761 cgccgctagc cattgaaaag aatgtcgagt tcaccacgat cagcggccgt tgccagcgtt
     2821 gatagaattc ggctggcgtg aaccgctgat tcttcgtcgt ttccgcggta aagacaaggt
     2881 cgcgatctct aaggcgggct gaaacataaa aggcgatgaa gggcgaaccg tcgaggctgt
     2941 caggcgacct gtaaacgtga accgatggcg gaagcgggcc gaaactcgta tcaacttttc
     3001 gccaacggag ctgtgcccgg gatgcaatgg gaatcaccag caggaggaga aaggtggcaa
     3061 ccggcttcag gttcacggta tatattttgg actaattggc ggcttgtttt tgccgcagcc
     3121 actcgtttcg gccatagccg gggacaacat ggcgctcgct gtggtaggaa gaacgcacca
     3181 gcggtccgct ctcgacataa tcgaatccca tttggtagcc tgcttccttc agttccgaga
     3241 actcgtcggg atggacgaag cgggttaccg ggagatgttt tttggacggc tggaggtatt
     3301 gcccgatggt gaggacatca acaccactct cgcgcaggtc ccgcatggtc tgaagcacct
     3361 cctccttccg ctcgccaagg ccaagcatga tcccgctctt cgttcgcatt ccccc
//
LOCUS       DATGKE010000384         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_486615, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000384 DATGKE010000000
VERSION     DATGKE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486615"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VKU83_06675"
     CDS             complement(<1..708)
                     /locus_tag="VKU83_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93272.1"
                     /translation="MKQFHALICIFFGLLPVLCPAQDLATNDHDTNRQNAIAAAEPPH
                     NGSIIAGEPIDSRSDWSKEEDRVLGSISKGKLMKMKGVTNELVDFLRDTCIGEGVYNP
                     VWHGEYFSSKNSPGAQLKFVLTCHFPDQNADLSITANDIQPLLDEMVVNGQHYITVRV
                     PSQVTDNGIYFGETGNGGAGQTKMWLITNGRGRMPFIAVTRREYLNQARAELMALVNS
                     IIAGWKMKVPVRSAAVQD"
     gene            1032..2441
                     /locus_tag="VKU83_06680"
     CDS             1032..2441
                     /locus_tag="VKU83_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93273.1"
                     /translation="MKPQVTDIFCGLLLILAPFAGNSQPAIEFASGSGPSGNGSVITN
                     QVITFQTNMFNPATGTYSPFSPTTTATFAISNQQYVLPATQNPNGGDISFGATNDPTG
                     KVLGSAPTFPAMDWISAPSNTDFSATAANVGAGIAVNMNYAVEIFTSTMGLFNAHAAT
                     NGRYYMGDLTITLNIPITNPVLHVVGLGGTYNALGFTTELQLVTTGITLSELSGSKEL
                     NVTPTAILNSASAPTATTGAGAASGSILVTGSSVTSLHFQIYMRGNGKTPTWSNGLEH
                     TGDAWLIGVSAQNTLVELPVGISSFTARPGQRAVSLQWTTASEQNSSYFDIQRSSTGT
                     SWTTIGRVPSAGNSDKPMQYEFVDNGPNSGANFYRLAEEDENQGLVYSPVRNVDFSGL
                     SASINWYPNPVHDRLTITSNAIIRSATLITADGRILREFDEFSSGQSLDLSSYPFGIY
                     FLMIRTADGASQTARIERD"
     gene            complement(2500..>2880)
                     /locus_tag="VKU83_06685"
     CDS             complement(2500..>2880)
                     /locus_tag="VKU83_06685"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HLI93274.1"
                     /translation="NEFGLNTVLPLVRRAALRKEDALRTYVEHIVVQVARTVAGLGAG
                     GKMLVTGGGAHNGFLIRQLSERLAAVGVEAVVPEGVIVDFKEALVMALIGVLRWREEN
                     NVFASVTGASRDSIGGAVWIGQEV"
BASE COUNT          655 a          851 c          728 g          646 t
ORIGIN      
        1 gtcctggacg gctgcggaac gaacgggcac cttcattttc cagccggcga tgatgctgtt
       61 gaccaacgcc attagctcgg cgcgggcctg gttaagatat tccctgcgag tgacggcgat
      121 aaaaggcatc cggccccttc cgttagtgat cagccacatc ttcgtctgtc cggcgccgcc
      181 attgcctgtc tccccaaaat agatgccgtt gtccgtaacc tggctgggta cccgaaccgt
      241 aatataatgc tgaccgttga cgaccatctc gtcgagcagc ggctggatat cattggcggt
      301 gatgctgagg tcggcgtttt gatccgggaa atgacaagtc aaaacaaatt taagctgcgc
      361 acccgggctg ttcttgctgg aaaaatattc gccatgccag acaggattat acacgccctc
      421 gccgatgcac gtgtcacgga gaaaatccac cagctcattg gtaacgcctt tcattttcat
      481 gagcttgccc ttgctgatcg agccgaggac ccggtcttct tcctttgacc agtccgacct
      541 ggaatcgata ggttcgccgg caataatgga tccgttgtgt gggggctcgg ccgctgcgat
      601 agcattttgg cgattggtgt cgtgatcgtt cgttgcaaga tcctgggcgg gacacaggac
      661 gggtagcagg ccgaaaaaga tgcaaatcag cgcgtgaaat tgtttcatga ggtccatttt
      721 tataggtgca cgatcgagag gtttagcgca ggacctggag ggtaagggag gctttttcgg
      781 ataccggaga ggagggaatt atcccggatt atagatttct taacaattac tggaccaaaa
      841 attaagggcc tgagttccaa attgttggtc tcatacccgt ttgcgttgac gtccctcccc
      901 cggaaaattc ttcccgggat cggccgggcc gcagcgttag tacgccgtca tacggatgac
      961 gatggcgaaa taagcgaaaa cccttgcaat tgtgggtttt cttgactatt ttgatcgtaa
     1021 atcccctacc catgaaaccg caagtcaccg atatcttttg cggattactg ctgattttgg
     1081 cgccgtttgc aggaaattcc caacccgcca ttgaattcgc cagcggctct ggtccctcag
     1141 gcaatggttc agtgataacc aatcaggtga tcacttttca gacgaacatg ttcaatccgg
     1201 ctacaggcac ctattcgcct ttcagcccga cgacgaccgc aacattcgcc atcagcaatc
     1261 aacaatatgt tttgcctgcc acccagaacc ctaatggcgg cgacatctct ttcggcgcaa
     1321 ccaacgaccc caccggcaaa gtcctcggct ccgcgccaac gttcccggcc atggactgga
     1381 tcagcgctcc gtccaacacg gacttcagtg ccacggcggc caatgtcggc gccggaattg
     1441 ccgtcaatat gaattacgca gtagagattt ttacgtccac aatggggctg ttcaatgcac
     1501 atgccgctac caacggccgt tattacatgg gcgacctgac gataacattg aacattccca
     1561 tcaccaatcc ggtccttcat gtggttggcc tcggaggtac gtacaatgcg ttaggcttta
     1621 cgacagaact acagctggta acgaccggga ttacattatc ggaactgtcg gggtcgaagg
     1681 agctgaatgt aacgcctacc gctattctca attcggcatc ggcgcctacc gctacgacgg
     1741 gggccggcgc ggcatccggc tccattctgg taaccggctc cagcgttacc agcctgcatt
     1801 tccagatcta catgcgcggc aatggtaaaa cccccacctg gtcgaacggt ttggaacata
     1861 ccggcgatgc ctggctgata ggcgtttccg cgcaaaatac gctggtagag cttcctgtcg
     1921 ggataagttc ttttaccgcg agacccggac aacgtgccgt atccctgcaa tggacgacag
     1981 cgagcgaaca gaactccagc tatttcgata ttcagcgtag ttccaccgga acatcgtgga
     2041 ccaccatcgg ccgcgtgccc tcagctggca acagcgataa acccatgcaa tacgaattcg
     2101 tcgacaacgg tccgaattcg ggtgcgaact tttaccgcct tgccgaagaa gacgagaatc
     2161 aaggtctggt gtattcacct gtccggaacg ttgacttctc cggcttgtcc gcatcgatta
     2221 actggtaccc caacccggtg cacgaccggt tgacgattac cagcaatgcg ataatccggt
     2281 cggcgacgct gattaccgca gacggccgca tcttaaggga attcgatgaa ttctcttcgg
     2341 ggcaatccct cgatctgagc agctatccgt ttggaatcta tttcctgatg atccggacag
     2401 ccgatggagc ttcccaaacg gccaggatcg agcgcgatta gggagctgct gcccgatcgg
     2461 cgaaaggcat tcgaaaggca tgcaaaagcg actccatgcc tacacctcct gcccgatcca
     2521 cacggctccg ccgatactgt cacgactcgc tcccgtaacg gaagcaaaaa cattattctc
     2581 ctcccgccat ctcaggacac cgatcagggc catgaccagc gcttccttga aatcgacgat
     2641 caccccttcc ggcaccactg cttccacccc tacggctgcc aacctttccg aaagttgccg
     2701 gatcaaaaac ccattgtgcg cacctccccc cgtcaccagc attttgcccc cggcaccgag
     2761 accggccacc gtcctggcga cctgcacgac aatatgttca acataggtcc tcaatgcgtc
     2821 ctcctttctg agcgctgccc ggcgcaccag cggcaacacc gtgttcaggc caaactcgtt
//
LOCUS       DATGKE010000385         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_373190, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000385 DATGKE010000000
VERSION     DATGKE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373190"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2458
                     /locus_tag="VKU83_06690"
     CDS             <1..>2458
                     /locus_tag="VKU83_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HLI93275.1"
                     /translation="LMPELVMPGYDNTLLAQTRAAAVRTQIEYGHEQGVPWGISEAAY
                     NSVDAALNYQYRAFGIPGLGLKRGLKDDLVIAPYATMLALMVAPAKACANLQRLESMG
                     ANGKYGFYESIDFTPGRLPRGKQQAIVQCFMVHHQGMSLLALACLLLDKPMQRRFLAD
                     LRFQATDLLLQERAPRTALLYSHTTDMAETHAATGEMPVRRLTTANTPVPAIQLLSNG
                     RYQVMITNSGGGYSRWNDLAVTRWREDPTRDNHGIFCYIKDLSNGVFWSNTWQPTLLA
                     AKEYESLFSQGHVEFRRRDQGIQTRTEIVISPEDDTEIRKVSITNKTTTTKILEVTSY
                     AEVVLASQAADEAHPAFGNLFVETEIRPEHEVILCTRRPRSREEKPPWLFHLMQAHDT
                     VVESVSYETSRMTFTGRGNTLAHPRAMDLDLLSGSQGPVLDPILSIRYRIRINPGRTA
                     VIDLIYGIAETKESCENLLHKYRDPYLKSRALELSWTHSQVMLRQINASESDAQLYNR
                     LAAYIIYPDPAFRAGTATIKNNLRGQSGLWGYSVSGDLPIVLLHVYDPDNLDFVKQLI
                     RAHAYWRLQGLSVDLVIWNEDYGSYRQALQERIQGLISSEAVAAPNNKPGAIFVRPAD
                     QLSTEDRILFESVARLILHDNQGTLPEQLDRPRNGKALAPLLEPKVLPPRPLPVPAAG
                     SENLLFFNGTGGFSADGKEYTILTDGSRTTPAPWVNVLANPGFGTVVSESGSAYTWAV
                     NAHEHRLTPWSNDPVSDGGEEAFYLRDEETGEFWSPAPYPVRGPANYRTTHGFGYSTF
                     SHAQNGITTEFTVFVDKSSPV"
BASE COUNT          506 a          794 c          708 g          450 t
ORIGIN      
        1 acctgatgcc cgagctggtg atgcccggct acgacaatac gctgctggcc cagacccggg
       61 cggccgccgt aagaacacaa atagaatatg gccacgagca gggcgtgcca tggggcattt
      121 cggaggctgc ctacaattcg gtggacgcgg ctttgaatta ccagtaccgc gcctttggca
      181 ttcccgggct tggattgaag cgtgggctga aggacgacct ggtgatcgcg ccctacgcta
      241 ccatgctcgc gctgatggtc gcgccggcga aggcctgcgc gaatctgcag cggctggagt
      301 cgatgggcgc caatggcaaa tacggattct atgaatccat cgactttacg ccgggacgcc
      361 tgccgcgggg aaaacagcag gccatcgtgc agtgctttat ggtacatcac cagggaatga
      421 gcctgctggc gctcgcctgc ctgctgctgg acaaacccat gcaacgccgc ttcctcgcgg
      481 atctccgctt ccaggccacg gacctcctgc tccaggaacg cgcgccccgg acagccctgc
      541 tctattcgca tacgaccgac atggcggaga cgcatgcagc caccggagaa atgccggttc
      601 gccggcttac caccgccaat acacccgttc ccgccattca gctgctgagc aacggccggt
      661 accaggtgat gatcaccaat tccggtggcg gctacagccg gtggaacgac ctggccgtta
      721 cccgctggcg ggaagacccg acccgtgaca accatggcat cttttgctat atcaaagacc
      781 tctcgaatgg ggttttctgg tcgaatacct ggcaacccac cctgcttgcc gccaaagaat
      841 atgaatccct gttctcgcag ggacatgtcg agttccggcg gcgcgatcaa ggcattcaaa
      901 cccggacgga aatcgtgatc tcgcccgagg acgacacgga aatcaggaag gtcagcatca
      961 ccaataagac caccaccacc aaaatcctcg aggtcaccag ctatgccgaa gtggtcctgg
     1021 ccagccaggc cgccgacgag gcgcatcccg cctttggcaa cctcttcgtc gaaaccgaga
     1081 tccggccgga gcacgaggtc atcctttgta cgcggcgtcc ccgctcgcgg gaggaaaagc
     1141 cgccctggct ctttcacctg atgcaggcgc acgacactgt tgtcgaatcg gtctcctatg
     1201 agaccagccg gatgacgttc accggcaggg gcaataccct tgcccatccc cgggcgatgg
     1261 atctggatct cttatccggc agccagggcc ccgtgctgga tcccattttg tcgatccgtt
     1321 accgcatccg gatcaacccg ggcaggacgg ccgtgatcga cctgatctat gggatcgccg
     1381 agacaaaaga atcctgcgaa aacctgctgc acaaataccg ggacccctat ttgaaaagcc
     1441 gggcgctcga gctatcctgg acgcatagcc aggtgatgct gcgtcagatc aacgcgtcgg
     1501 aatccgacgc gcaactctac aaccggctcg cggcctacat tatttatccc gaccccgcct
     1561 tccgcgccgg aacagccacg atcaaaaaca atttacgcgg ccagtcgggc ctctggggat
     1621 atagtgtctc cggcgatctt cccatcgtgc tgctgcatgt gtatgatccg gacaacctgg
     1681 actttgtgaa gcaactgatc cgggcgcatg cttactggcg gttgcaggga ttatcggtag
     1741 acctggtgat ctggaacgag gattatggtt cctaccggca ggccttgcag gaaaggatcc
     1801 agggcctgat ctcctccgaa gcggtggccg cgcccaacaa taagcccggc gccattttcg
     1861 tccggcccgc cgaccagctg tcgacggaag accggatctt gttcgaaagc gtcgcgcggt
     1921 tgatcctgca cgataaccag ggcaccctgc cggaacagct ggaccggccg cggaacggga
     1981 aggcccttgc ccccttgctg gagccaaagg ttttgccgcc ccgccccctg cccgtcccgg
     2041 ccgccggcag cgaaaacctg ctgttcttca atggcaccgg cggtttttcc gccgacggaa
     2101 aagaatatac gatcctcacc gacggcagcc ggacgacgcc ggcgccctgg gtcaacgtcc
     2161 tggccaaccc gggattcggc accgtggtct cggaaagcgg atccgcctac acctgggccg
     2221 tcaatgcgca cgagcaccgg ctgaccccct ggagcaacga tccggtgagc gatggcggtg
     2281 aagaggcctt ttatctccgt gatgaggaga cgggcgaatt ctggtcaccg gctccctacc
     2341 ccgtccgcgg tccggcaaat taccgcacga ctcacggctt cggctattcg acgttcagcc
     2401 atgcgcaaaa cgggataacc actgaattta cggtgttcgt ggacaagtcg tcgccggt
//
LOCUS       DATGKE010000386         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_15729, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000386 DATGKE010000000
VERSION     DATGKE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15729"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VKU83_06695"
     CDS             <1..375
                     /locus_tag="VKU83_06695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HLI93276.1"
                     /translation="TGLEVNALGGEPGVRSARYAGDNKSDRDNIELLLRRLGDNPDRR
                     ARFRTVISLIWNGDYHEFEGICEGTILQAPAGNNGFGYDPVFVPCGESRSFAQLSLEE
                     KNRYSHRRKAADQLLAFLRQSK"
     gene            385..816
                     /locus_tag="VKU83_06700"
     CDS             385..816
                     /locus_tag="VKU83_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLI93277.1"
                     /translation="MPKTRLVLPERFPFTTQIPVRVTDLNFGGHVGNDSVLSLIHEAR
                     AQFLAHCGYSEMDIAGVGLIMTDVVIEFKTELFYGDSLWASVAAAEFSRVGFDLYYRL
                     EKKLGEKMQSVAVARTGMICYDYKVRKIASVPKEVCTRLLA"
     gene            complement(828..1562)
                     /locus_tag="VKU83_06705"
     CDS             complement(828..1562)
                     /locus_tag="VKU83_06705"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLI93278.1"
                     /translation="MRRYGLIGYPLTHSFSERFFTEKFEREGIKDCTYSNYPIPQIGA
                     LPEVLADPSLCGLNVTIPYKQQVIPFLQELSPVVEAIGACNCIRIDKGGLAGHNTDVT
                     GFERSLTRKLAPQHRQALILGTGGAARAVAYVLLKLGISYRLVSRNPRPDTGDLGYDA
                     IDAEILRDFLLIVNTSPVGMYPRVDECPPLPYEALTTRHYLFDLVYNPARTAFLRHGE
                     ARGSIVENGHEMLVIQAEESWRIWNA"
     gene            complement(1580..2344)
                     /locus_tag="VKU83_06710"
     CDS             complement(1580..2344)
                     /locus_tag="VKU83_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HLI93279.1"
                     /translation="MNFKLSQMPDRLAKPRQHGITMVMDKGLSIEEAKNFLSVAHPHV
                     DIVKLGFGTSFVTPKLREKIEVYQSYGLPVYFGGTLFEAFLIRNQFDDYIAILKDYGI
                     KHIEVSDGSITIPHTEKCGYIEKLTRFGVVLSEVGSKDAAHIIPPYKWIELMSAELSA
                     GASYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPAERILWEAPQKAQQLYFIELI
                     GCNVNLGNIAPAEVIAMEAMRVGLRGDTFHLFLEKQ"
     gene            complement(2403..3824)
                     /locus_tag="VKU83_06715"
     CDS             complement(2403..3824)
                     /locus_tag="VKU83_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLI93280.1"
                     /translation="MQENPYHQDREEMKELLKQYLDLKNGKQRTFLEEEAFERIIDYF
                     DDTEDLTQAVEAVELGIEQYPYSSALMVKKADLLIATRHYEEALAILEQVELLDSSDI
                     NLYILKTDAYLALDQPQKAAALLESALSHFEGEERVELLFELADVYDDYEEFDKIFDC
                     LKWILEQEPDNEEALYKICFWTDFTGRNEESIRLHQQIIEDKPYSELAWFNLAAAYQG
                     LKLYEKAVDAYKYALAIDEKFDYAYRNMGDAFIRLRKYKEAIESLEKVLELSRPEDVI
                     YEAIGHCYDKQDNYAQARFYYRKASHLSPDDSKLYYKLACTYINEGLWNQAVRQLEAA
                     MRIHGMQPEYNLAMGECKMQLGEFKEAIQFFSNVVRVRPRNISSWEALIRCLYNAEYY
                     EEAMEQVQAAIKITGGKPIFLFYFSAIQFAIGKTRDALLQLENAMSKAPKMLKKLLEL
                     NPAILQYQPVVDIVARYKKNKSI"
     gene            4214..4564
                     /locus_tag="VKU83_06720"
     CDS             4214..4564
                     /locus_tag="VKU83_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93281.1"
                     /translation="MRKALISASLLAVVGLALASKGGGGDKKANMPLKTNFVPIRTTT
                     GFTLKVGPTYTGSMITGAEKTPDYVSVHTLITYQHGNTTYIMPHTYRFNSNVYMRNTQ
                     GSNLSLLDLRINMH"
     gene            complement(4616..>5187)
                     /locus_tag="VKU83_06725"
     CDS             complement(4616..>5187)
                     /locus_tag="VKU83_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93282.1"
                     /translation="GVSGSHPGQAWKAKDDSSERALHTVYIKGNKSRSEISSPLFSTA
                     AIYDGNTGFGVILREVSGQKLLIRMNPDNWAERNRMWEGIVYHNTNETREIAGYKCMK
                     AVGQSRSGLMITVFYTREIVPENRTYDPLFRNLDGLPLEYELSNGTYRIRYRIARINL
                     NPVPGSKFDTPTSGYREMRYEDSKKMNLN"
BASE COUNT         1158 a         1488 c         1275 g         1266 t
ORIGIN      
        1 accgggcttg aagttaacgc gctcggcggc gagccaggcg tccgcagcgc acgctacgcg
       61 ggcgacaaca agtccgaccg ggataatatc gaactgcttt tgcgaaggct gggcgacaac
      121 cccgatcgcc gcgcccggtt ccgcaccgtg atctccctga tctggaatgg tgactatcac
      181 gagttcgaag ggatatgtga gggcacgatc ctccaggctc ctgctggaaa taacggcttc
      241 ggttatgacc cagtatttgt cccctgcggc gagtcgcgca gtttcgccca gctttccctc
      301 gaagagaaaa accgctatag ccatcgccgc aaagctgccg atcagctgct ggcctttctg
      361 cgacaatcaa aatgacgcct tcatatgcct aagaccagac ttgtcctgcc ggagcgtttc
      421 ccgtttacaa cgcagattcc cgttcgggtg accgacctga atttcggtgg ccacgtcggc
      481 aacgactcgg tcctttcgct catccacgaa gctcgcgccc aattcctggc gcactgcggt
      541 tacagtgaga tggatattgc cggcgttggt ctcatcatga ctgacgtagt tatagaattc
      601 aagacggagc ttttctatgg cgatagccta tgggcctccg tggccgctgc ggaattttcc
      661 cgggtcggat tcgacctata ttaccggctg gaaaaaaagc tgggggagaa aatgcaatct
      721 gtcgccgtcg cccggacagg gatgatctgc tacgattaca aagtccggaa gatcgcctcc
      781 gtcccgaaag aagtctgcac gaggctgctt gcgtaaagct tccgccgtca ggcattccaa
      841 atgcgccagc tttcctcagc ctggatcacg agcatctcat gtccgttttc aacgatcgaa
      901 ccccgcgcct ccccatgccg caggaacgct gtcctggcag gattgtacac caggtcgaat
      961 aaatagtgcc gggtggtcag ggcttcataa ggaagcggcg ggcattcgtc cacgcgcggg
     1021 tacattccaa cggggctcgt gttgacgatc agcaaaaagt cgcgcaggat ctccgcatcg
     1081 atcgcgtcat agccgaggtc gccggtatcg ggtcgcggat tccgcgaaac cagccggtag
     1141 gaaatcccaa gttttagcag cacatatgcc accgcccgtg cggcgccacc cgtacccaaa
     1201 atcagcgctt ggcggtgctg cggcgccaac tttcgggtga gcgaccgttc gaaaccagtc
     1261 acgtcggtat tatgacccgc gaggccaccc ttatcgatcc ggatacagtt gcaggccccg
     1321 attgcttcta ccaccgggct taactcctgc aaaaaaggaa ttacctgctg cttgtaggga
     1381 atggtgacat tgaggccgca cagggaaggg tcggccagca cttccggcaa cgccccgatc
     1441 tgaggaatcg ggtaattgga ataggtacaa tcctttattc cttcccgttc gaatttttcc
     1501 gtaaagaacc gctcggagaa agaatgcgtc agcggatatc ctatcaaccc ataccgtctc
     1561 atatttgaat tgggccccct tactgttttt ccaggaaaag atggaaggta tccccgcgca
     1621 acccgacgcg cattgcctcc atcgcgatta cttccgccgg tgcgatattg ccgaggttga
     1681 cgttgcaccc gatcagctcg atgaaataaa gctgctgcgc cttctgcggc gcctcccaga
     1741 ggatccgttc cgcggggatc tgggttagga tctcctgcac caggccctcg cgcacctccc
     1801 cgcttccgcg gtaaatccca acattccctg cctcgcgggc ttccgcaata acataggaag
     1861 cgccggcgct cagttcggcc gacatgagtt cgatccattt atagggaggg atgatatggg
     1921 ccgcgtcctt gctacccact tcgctcagga cgacgccgaa ccgggtcagc ttttcaatgt
     1981 atccgcattt ttcggtgtgc gggatcgtga tggagccgtc acttacttcg atatgtttga
     2041 taccataatc tttcaggatg gcgatataat cgtcgaactg gttccggatc aggaatgcct
     2101 cgaataatgt gccgccgaaa tagacgggca ggccgtacga ttgatagacc tcgatcttct
     2161 cccgtaattt gggcgtgaca aaggaagtcc caaagcccaa tttgacgatg tctacatggg
     2221 gatgcgccac actcaggaaa tttttcgcct cttctatact caaccccttg tccatgacca
     2281 ttgtaattcc atgctgacgc ggcttcgcca gcctgtcagg catctgcgat agtttgaaat
     2341 tcatcctcgt tttgttttga gttccggtgt gcgtaacggc gcaaatgtag gaaaaacggc
     2401 gatcagatgg atttgttctt tttgtaccga gcgacaatat cgaccaccgg ttggtactgc
     2461 aagatggcgg ggtttagctc gagcagcttc ttgagcatct tcggcgcctt cgacatggca
     2521 ttctcgagtt ggagcaacgc gtccctggtc ttgccgatcg cgaactggat ggccgaaaaa
     2581 taaaagagga agatcggctt gccgccggta atctttatcg cagcctggac ctgctccatc
     2641 gcctcttcat aatattccgc attgtaaagg caacggatca acgcctccca actggaaata
     2701 ttgcgcggcc tgacgcgcac cacattcgag aagaactgga tcgcctcctt gaattctcca
     2761 agctgcatct tgcattcgcc catcgccagg ttatattcgg gctgcatccc gtggatccgc
     2821 atcgccgcct ccagttgccg aacggcctgg ttccataagc cctcattgat ataggtgcag
     2881 gcaagcttgt aatagagctt gctgtcgtcg gggctcaggt gcgaggcctt ccggtaatag
     2941 aacctggctt gcgcatagtt gtcctgctta tcataacagt gaccaatagc ctcgtaaatg
     3001 acatcttcgg gcctcgacag ctcgagtact ttctccagcg attcaatggc ctccttgtac
     3061 ttgcgcagcc ggataaaggc atctcccata ttacggtagg catagtcgaa cttttcatcg
     3121 atggccagcg catacttgta ggcatccacg gctttttcat aaagcttcag gccctggtag
     3181 gcggcggcaa ggttgaacca cgccagctcg ctgtatggct tgtcctcgat gatttgctga
     3241 tggagcctta tactttcttc attcctacct gtgaaatccg tccaaaagca gatcttatac
     3301 aacgcctcct cgttatccgg ttcctgctcc aggatccact tcaggcaatc aaagatctta
     3361 tcgaactctt cgtagtcatc atagacgtcg gccagttcaa acaacagttc cacccgctct
     3421 tcgccttcga aatgggacaa tgcgctctcg agcagggccg cagccttctg cggttggtcc
     3481 agcgcgagat atgcatccgt cttgagtata tagagattga tgtcggagct gtccagcaat
     3541 tcgacctgtt ccaggatcgc caacgcttct tcgtaatgcc gcgtcgctat cagcaggtcc
     3601 gcttttttca ccatcaaggc tgaagagtat ggatattgct cgatccccag ttcaactgcc
     3661 tcaacggcct gtgtaaggtc ctccgtatcg tcaaaatagt cgatgatccg ctcgaatgct
     3721 tcctcctcca aaaaggtacg ctgcttccca ttcttcagat ccaggtactg cttcaacaat
     3781 tctttcattt cctcgcggtc ctgatggtat ggattttctt gcatgtgata aacgttgttg
     3841 taagttacac tatttgaact gcttcgccga aaaattatca gaacctgcag gcgcggcaaa
     3901 tgacgaacaa gccacgcaaa tggacggctc ctatcaggcc aataacaact ccgggccagg
     3961 taatccttca ttctatccct taacacaacc gtttttggac cgcgcggtga cccaggccgg
     4021 cgtatccgcg gattcccccg gcagtacgac tccgttcgtt ggcgcccaaa aatcgataag
     4081 atgtaaaaat ttagttatca atcagttaat gctttattaa atttagtgac gaacggttaa
     4141 aaattaccca aattatttcc atacctttgg ttatagaatg ttaaatctga gtaccataga
     4201 actgcggaaa ttgatgagga aagccctgat cagcgcttcc ctgcttgctg tggtcggtct
     4261 cgcattggcc agcaaaggtg gcggtggcga caaaaaggca aacatgccct taaaaaccaa
     4321 cttcgtacct atcaggacca ctacaggatt tacattgaag gtcgggccga cctatacggg
     4381 cagcatgatc accggcgccg aaaagactcc tgactatgtt tccgttcaca ctttgattac
     4441 ttaccagcac ggtaatacga cctatatcat gccgcacacc taccgcttca atagcaatgt
     4501 gtatatgcgg aacacgcaag gcagcaatct cagcctgctt gacctgcgga tcaacatgca
     4561 ctgatccgcc tgcttcgatt catcccggat taaatttgcc agcactcctc tgcggtcaat
     4621 tcaaattcat tttcttgctg tcctcatagc gcatttcccg gtatccgctg gtcggggtgt
     4681 caaacttcga tcccggtacc ggattcagat tgatccgggc gatcctgtac cggatgcgat
     4741 acgtgccgtt gctcagctca tattccagcg gcagcccgtc aagattccgg aacagcggat
     4801 catacgtccg gttctcagga acgatctccc tcgtatagaa gaccgttatc attaaccctg
     4861 atctcgactg ccctaccgcc ttcatgcatt tgtatcccgc aatctccctc gtttcgttcg
     4921 tattgtgata aacaatgccc tcccacatcc ggtttcgctc cgcccaattg tccggattca
     4981 tccggatcag caatttttgc ccgctcacct cccggaggat aaccccgaaa ccggtattcc
     5041 cgtcatagat ggccgcagtg gaaaaaagcg ggctcgagat ttccgaccgg ctcttgtttc
     5101 ccttgatgta tacggtatgc aacgcacgct cgctgctgtc gtccttcgcc ttccatgcct
     5161 gtcccgggtg gctgccggaa acgccca
//
LOCUS       DATGKE010000387         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_864612, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000387 DATGKE010000000
VERSION     DATGKE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864612"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3227)
                     /locus_tag="VKU83_06730"
     CDS             complement(<1..>3227)
                     /locus_tag="VKU83_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLI93283.1"
                     /translation="SRGFMWFGTREGLNKYNGYTFTVYKNEAGDDKSLANNFVYDMVE
                     DAAGNLWIATWGGGLDRYDRRTDQFTHFRHNPADPASIASNLVLALLKDSRGTIWLGT
                     ENGGLDRMDTVGHFIHYQNRPGDPTSLSDNLVKHLYEDEDHRIWVGTGNGGLNVLDQK
                     TGIFHHYLHSDKDPGSVRSNYVTIVYGDSHHRIWVGTGSGLDRFDRATGKFIHYPGDS
                     NNPDSGVTSLLVNSMAEGPAGNIWVGTENDGLVILNPVTNAVYHYRHDDADPASLTTN
                     SLWSIYMDSKKNMWIGSFTGGLELVSRDARKFIHYKHNSFPYSLSSNQVLCLFEDSRQ
                     NLWVGTDGGGLNLMDRKTGHFKHFLHVPGNASTIGGNYVLKVIEDHDGNLLVGTWSDG
                     MTIFDPRHNTFRQFRHQPRDSSSLTSNNVWTLCEDRQHQIWAGTYGGGVNVYDGVHRG
                     WVHYRHSAADPGSLADDKVHTITQDHEGRIWIGTDGGGMDLFNPLTHKFAHYRHIADV
                     NSLCSDYINNIYETKSGDLWISTTEGLSRFEVNANRFTSYTMRDGLPDNVIFGVLEDS
                     LGYLWISTNNGLCELDYRHRRATNFTAADGLQANEFKEQAFCSTRSGLMYFGGINGFN
                     VVSPGHIPREPFDPPLVMTSFQVFNQELPIGQHGDGLSLDADISETRHITLPHSASVI
                     SLTFASLNYTDPERKKYAYMLAGFDKDWNYVGSRRSATYTNLDPGTYTFRVKGYNNDG
                     QWSDKILSLELEIRPPVWMTWWFRVLAALFVVGGVISFYRLRIRTINAQKKELERQVQ
                     ERTERLVILSQEEHKAREEAEQANRAKTVFLATMSHEIRTPMNGVIGMASLLAETKLT
                     RQQQEYNSTIMSCGESLLSVLNDILDFSKIDSGKMEIEQQDFDLRQCIDGVLDIFYTK
                     VNQSGLRLSCWIEDGVPPHLVGDALRLRQVLINLVSNAVKFTERGEIKVGVSQLRKDS
                     NTGEIELAFEVRDTGIGIPAEKIDMLFKSFSQVDSSTTRKYGGTGLGLAISEKLVSLM
                     GGRIAVQSRVGQGTIFTFTIRTRAGYVQPNIPTPGQRMMSKAFAA"
BASE COUNT          665 a          972 c          819 g          771 t
ORIGIN      
        1 cagccgcgaa tgccttgctc atcatccgct gtccaggggt cggaatattt ggctggacgt
       61 aaccggcccg ggtccggatc gtaaacgtaa aaatcgttcc ctgcccgacc ctgctctgaa
      121 ccgcgatccg gcctcccatc agcgatacca gcttttcgct gatcgccaat cctagccccg
      181 tgcccccgta ttttcgggtc gtcgacgaat cgacctggga gaaggatttg aagagcatgt
      241 cgatcttttc cgctgggatc cctatcccgg tatcgcggac ctcgaaggcc agttcgatct
      301 cgcctgtatt tgaatccttg cgcaattggg aaacgccgac cttgatctcg ccgcgttcgg
      361 taaactttac ggcattgctc accaggttga tcagcacctg ccgcagccgc aacgcatccc
      421 caaccaggtg cggcggcacc ccatcctcta tccagcagga cagccgcaag cccgactgat
      481 ttactttcgt gtaaaatata tccagcacgc catcgatgca ttgccggagg tcaaagtcct
      541 gctgctcgat ttccatcttc ccggaatcga tcttggaaaa gtccaaaata tcattcaata
      601 cgctcagcag gctttcaccg cagctcataa tggtcgaatt gtattcctgc tgctgacgtg
      661 tcagcttcgt ttcggcaagc aagctggcca tcccgatcac cccgttcatc ggcgttctga
      721 tctcatggct catcgttgcc agaaaaaccg ttttcgcccg gttggcctgc tcggcttcct
      781 cccgcgcctt gtgttcttcc tggctcagta tcaccaggcg ctccgtccgt tcctgcacct
      841 ggcgctccaa ttccttcttt tgtgcgttga tcgtccggat ccggagccgg taaaagctga
      901 tcaccccgcc caccacaaat agcgccgcca agactctgaa ccaccacgtc atccagacgg
      961 gcggccgaat ttccagctcg aggctcagta ttttatccga ccattgccca tcattgttat
     1021 agcctttgac gcggaaagta taggtcccgg ggtcgaggtt tgtgtacgtt gcggaacgcc
     1081 tcgagccgac ataattccaa tccttgtcaa atcccgccaa catatatgcg tactttttcc
     1141 tctccggatc ggtatagttc aacgaagcaa aagtcaggga gatgaccgac gcactgtgcg
     1201 gtaaggtaat atgcctggtc tcggagatgt ctgcatcgag cgatagccca tcgccatgct
     1261 gcccgatcgg cagttcctgg ttgaatacct ggaaactcgt catgacgagt ggcggatcga
     1321 acggctcccg gggaatatgt ccgggcgaga cgacattgaa tccattgatg ccgccaaagt
     1381 acatcagccc cgaccgggtt gaacaaaaag cctgttcctt gaattcattt gcctgcagac
     1441 cgtccgccgc ggtaaaattg gtcgcacgcc ggtgccggta atccagctcg cacaggccgt
     1501 tgttggtgct gatccacagg tagcccaggg agtcctcgag gactccaaaa atgacattat
     1561 ccggcaagcc atcccgcatc gtgtaggacg tgaaccggtt cgcattgacc tcaaaccggc
     1621 tcaaaccctc ggtcgtactg atccacaagt cgccgctctt cgtttcgtaa atattattaa
     1681 tatagtcgct gcacaggctg ttcacatctg cgatgtggcg atagtgcgca aatttatggg
     1741 tcagcggatt gaagaggtcc atgccgcccc cgtcggtgcc gatccatatc ctgccttcgt
     1801 ggtcctgcgt gatcgtatgt accttgtcgt cggccaggct tcccggatcc gccgcgctat
     1861 gacggtaatg cacccagccg cggtggaccc catcgtagac attgacgcct ccgccgtatg
     1921 tcccggccca gatctggtgc tgccggtcct cgcaaagcgt ccagacattg ttactcgtca
     1981 ggctggagga gtcccgcggt tgatgcctga actgccgaaa agtattatgc cggggatcga
     2041 agatcgtcat cccgtccgac cacgtcccta ccaataagtt gccgtcatga tcttcaatca
     2101 ctttaaggac atagttgccg ccgatcgtac ttgcattccc tggcacatgc agaaaatgtt
     2161 tgaaatggcc tgtcttccgg tccatcaggt tcaggccgcc gccatcggtc cccacccaga
     2221 gattctgcct ggaatcctcg aaaaggcata atacctggtt actgctaagg ctatagggaa
     2281 aggaattgtg tttgtaatgg ataaactttc gcgcatcccg gctgaccagt tcgaggccgc
     2341 ccgtaaacga cccgatccac atgtttttct tcgagtccat gtaaatcgac catagcgagt
     2401 tggtcgtcag gctcgcaggg tccgcgtcat catgcctgta atggtaaacg gcattcgtaa
     2461 ccgggttcag gatgaccaaa ccgtcatttt ccgttccgac ccagatattg ccggccggac
     2521 cctccgccat ggaattgacc agcaaactgg tcactcccga gtccggatta ttgctgtcgc
     2581 cagggtagtg gatgaacttg ccggtcgccc tatcgaaacg gtccaacccg ctgccggtgc
     2641 ccacccagat ccggtgatgg ctgtctccat agacaatcgt gacatagtta ctgcggacgg
     2701 accctggatc tttgtcgcta tgcaggtagt ggtggaagat gcccgttttc tgatcgagaa
     2761 cattcaatcc gccatttccg gtccccaccc agatgcggtg atcctcgtct tcatacaggt
     2821 gcttgaccag gttgtccgag aggctggtcg ggtcgcccgg ccggttttga tagtgaatga
     2881 aatggcccac cgtatccatc cggtccaatc caccgttttc cgttccgagc cagatcgttc
     2941 cccggctgtc tttcaacaat gcaaggacga ggttgctcgc gatactggcc gggtcggccg
     3001 gattgtgcct gaaatgcgta aactgatccg tcctgcggtc ataccggtcc aggccgccgc
     3061 cccaggtggc gatccaaaga ttgcctgcgg cgtcttcgac catgtcgtag acaaaattat
     3121 ttgcgaggct cttatcgtcc cccgcctcgt ttttgtatac agtgaaagtg tagccgttgt
     3181 atttgttcag cccttcccgg gtaccgaacc acatgaaacc gcggctg
//
LOCUS       DATGKE010000388         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1153740, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000388 DATGKE010000000
VERSION     DATGKE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153740"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VKU83_06735"
     CDS             <1..847
                     /locus_tag="VKU83_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93284.1"
                     /translation="NMNTKRPDVRTAIMLLESHFQDLRTCNMDKLRRQLGLEDDEIRI
                     VLNLLAHLSTRPFNPEATSIQANPSILPDFIIFEEEDALEIALYRQRSSNLHISQSWM
                     EMVQSSAQNTSMDKAAKQYLRSKLSSAQWFINAIQQRESNMLKIMRAIVDMQYEYFKY
                     GDILLLKPMKLKDIADQVKVDISTVSRLTCNKYAETPFGTILLKDLFTEGIVNQEGNN
                     ISNKVIQNIISEVIQGEDKKAPYTDRQLVTILAQKGYSIARRTVAKYRELMNIPVAQV
                     RGLWE"
     gene            887..1663
                     /locus_tag="VKU83_06740"
     CDS             887..1663
                     /locus_tag="VKU83_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HLI93285.1"
                     /translation="MRSIWTGSIGFGLVNIPIKMYSAVQDSNLDLDMLDKKDHAKIRF
                     KRVNEETGKEVPWENIVKGYLLDDRYVILTDEDFEKASPEKSKVITINEFVTETEIDG
                     IYYESPYYLEPDKSGVKAYALLRDALAKTGKAGFGSFVLRNKEGLCLIKPLDNVLVLN
                     RIRWAQEIRSREDLNVPAESSKPAEMKMAVQLIEQLSGPFEIEKYKDTYADELMKLIK
                     AKAKGVKPAAPTLRVVHKKGKEDLLTQLKASLQGSKRRVS"
     gene            complement(1677..2600)
                     /locus_tag="VKU83_06745"
     CDS             complement(1677..2600)
                     /locus_tag="VKU83_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLI93286.1"
                     /translation="MKQKRPGLKAYWQTIKKAAGDFNQDNAFKLAASLSYSMIFSIGP
                     LLIVVISLAGIFWGRQAVEGKIYLQIKDLVGGPAALQIQDIIRYIQQSKHTVAGAIIG
                     GAVLIIGATGVFTEIQGSINYMWAIQAKPRKGWLYLLMSRLISFSLIVAFGFISMVSL
                     VINSLMDLLSDYLVKYFSHLTIYLFYVVNLLLVLGAIVLLFMIIFRILPDAIIRWRDA
                     FTGAVFTGILFMIGKLLISVYLGHSNIGHMYGAAASMMVILVWVYYSSIILYFGAEFT
                     KIYAMRNGGGIRPDENAVFILKRESRVLEKE"
BASE COUNT          766 a          778 c          715 g          549 t
ORIGIN      
        1 gaatatgaac accaagcgcc ccgacgtgcg gaccgccatt atgctcctcg aaagccattt
       61 ccaggacctg cgcacctgca atatggataa gctgagacgg caattgggtc tcgaagacga
      121 cgagatccgg atcgtgctga acctgctggc gcacctcagt acccggccgt tcaatcccga
      181 agccacaagc atccaggcca acccgtccat tttaccggat ttcattatct tcgaggagga
      241 agacgcactg gagatcgcgc tctaccgcca acgctcctcc aacctccata tcagccagtc
      301 gtggatggag atggttcaga gttcggcgca gaacacctcg atggataagg cagcgaagca
      361 gtaccttcgc agcaagcttt cctccgcgca atggttcatc aacgccattc agcaacggga
      421 gtcgaatatg ctcaagatca tgcgcgcgat cgtcgacatg cagtatgaat atttcaaata
      481 cggcgatatc ctcttgctga agcccatgaa attaaaggac atcgcagatc aggtcaaagt
      541 cgatatctct acggtctccc ggctgacctg caacaaatac gcggagacgc cgtttggaac
      601 cattctgctg aaggaccttt ttacggaggg catcgttaac caggaaggaa ataatatcag
      661 caataaggtg atccagaaca tcatcagcga ggtcatccag ggagaagata aaaaagcgcc
      721 ctataccgac cgtcagctgg tcaccatcct ggcgcaaaaa ggttacagta tcgcccggcg
      781 cacggtcgcc aagtaccggg agctgatgaa tattccggtg gcgcaggtgc gcgggctttg
      841 ggagtgagtc cctcccacga ccgtttgaca agcaaaatct aaccatatgc gatcgatctg
      901 gacggggtcc atcggcttcg gcctggtgaa tatccccatc aaaatgtaca gcgcggtaca
      961 agacagcaac ctcgacctgg acatgctcga taaaaaggac cacgccaaga tcaggttcaa
     1021 acgtgtcaac gaggaaaccg gaaaggaggt gccgtgggag aatatcgtca agggctacct
     1081 gctcgacgac cggtacgtca tcctgaccga tgaagatttt gaaaaggcaa gccccgaaaa
     1141 atccaaggtg attacgatca acgaatttgt gaccgaaacc gaaatcgacg gcatttacta
     1201 cgaatcaccc tattacctgg aaccggacaa atccggcgtt aaagcctatg cgctgctgcg
     1261 cgacgccctc gctaaaaccg ggaaggcggg cttcggcagc ttcgttctcc ggaacaagga
     1321 agggctctgt ctcattaaac ccctcgacaa cgtcctggtg ctgaaccgca tccgctgggc
     1381 gcaggagatc cggtccaggg aagacctcaa cgtcccggcg gaatcgagca aacccgcgga
     1441 gatgaagatg gccgtacagc tcatcgagca gctgtccggc ccgttcgaga tcgaaaaata
     1501 caaggatacg tatgccgacg agttaatgaa gctgatcaag gcaaaggcaa agggtgtcaa
     1561 acctgccgcg cccaccctcc gggtggtgca caagaaaggg aaggaggacc tgctcaccca
     1621 gctgaaggcc agcctgcagg ggagcaaacg ccgcgtttcc tgagcgggat gccctgctac
     1681 tccttctcaa gtaccctgga ctcccgtttt aaaataaata cggcattttc atccggcctg
     1741 atgccgccgc cgttacgcat cgcatagatc ttggtaaatt ccgccccgaa atataaaatg
     1801 atcgatgaat aatataccca cacgaggatg accatcatcg acgccgccgc cccatacatg
     1861 tgcccgatat tcgaatgacc gaggtagacg ctgatcaaca gcttcccgat cataaacaat
     1921 atcccggtga acaccgcgcc ggtaaacgcg tcccgccaac gaatgatcgc atcaggcagg
     1981 atccggaaga tgatcatgaa caggagaacg atcgccccaa gcaccaacaa caggttgacg
     2041 acataaaaca aataaatcgt aaggtgagaa aaatatttca ccagataatc actcagcagg
     2101 tccatgaggg agttgatcac cagggacacc atggagatga acccgaaggc aacgatcagc
     2161 gaaaaggaga tcaggcggct catcaacagg taaagccagc ccttgcgggg ctttgcctgg
     2221 atggcccaca tgtagttgat cgaaccctgg atctcggtaa atacgcccgt agcgccgatg
     2281 atcagcacgg cgccgccgat gatggcgccg gccaccgtat gcttggattg ctggatgtac
     2341 cggatgatat cctggatttg cagcgcggcc ggcccgccaa ccaggtcctt gatctggaga
     2401 tagatcttac cttccacagc ctggcggccc cagaagatgc ctgccagtga aataacgacg
     2461 atcagcagcg gaccgatgga aaagatcatc gagtacgaca gacttgccgc cagcttaaaa
     2521 gcattgtcct gattgaagtc gcctgccgcc ttcttgatgg tttgccaata tgccttcaga
     2581 cccggtcgtt tctgcttcat taccgtgatc cagcaagatc gttgccagag tgcggtccct
     2641 tgtcccggcc tcaaagtgcg aaatccagga tattgtagac attgttcccc aggagcgcat
     2701 gcccgccgcg gaattccgtt tacaaaataa ttggcaagca attttcaata catccataga
     2761 aatatattcc tggcactaaa aacaattttt atgagtggat tactttat
//
LOCUS       DATGKE010000389         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_141048, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000389 DATGKE010000000
VERSION     DATGKE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141048"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VKU83_06750"
     CDS             <1..318
                     /locus_tag="VKU83_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93287.1"
                     /translation="ILFKTTVKIAIGCIFLFAIGCRRTIPKVNVPLELKKAMLSYLKK
                     QPNYDSTRVRFEVRDVYYYADTNLYRCEFKVHMVVPSRNVDTTGMMDGTVSKDFKTVH
                     RTD"
     gene            complement(387..965)
                     /locus_tag="VKU83_06755"
     CDS             complement(387..965)
                     /locus_tag="VKU83_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLI93288.1"
                     /translation="MNRRDAITHVSLLLGGTILGAEAFLSGCTNPPEQKLGGMGTNFS
                     NSDVAFLDEVAETILPKTNTPGAKDAKVGEFMTVIVRDCYTQPDQKVFLDGMQKLDDA
                     SRKRNGKAFLDSTPEQRHDLLVDLDKESKEYAKTKKKEDPQHYFRMMKELTLWGYFTS
                     KEGATEALVYVPVPGRYEGCIDLKKGQKAWAT"
     gene            complement(992..>2083)
                     /locus_tag="VKU83_06760"
     CDS             complement(992..>2083)
                     /locus_tag="VKU83_06760"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLI93289.1"
                     /translation="YKGKRHMIIGRTANLTQPLGTRRCEFRNKCWLGCPFGGYFSTQS
                     STLPAARATGRLTLRPWSIVTKILYDKDKKRATGVEVLDAETNKTYEFKSKIVFLNAS
                     TLNSAWVLMNSATDVWPDGLGSSSGELGHNLMDHHLGVGAGGTVEGYEDKYYYGRRAN
                     GIYIPKYRNLYGEKRDYIRGFGYQGGGGRQGYQHEVAEYSIGKEYKEALTEPGPWTMS
                     IGAFGEILPYHENKVTLDKTRKDKWGLNILAIDCELKENELKMRKDMEADAVEMLTIA
                     GVKNVRSRSDNPFPGRGIHEMGTARMGRDPKTSVLNKWNQVWDAPNVFVTDGSFMASA
                     NCCNPSLSYMAFTARAADHAVSELKKQNI"
BASE COUNT          405 a          611 c          523 g          544 t
ORIGIN      
        1 attttattta aaacgacggt taaaattgct atcggttgca ttttcctttt cgctattggg
       61 tgccgccgga ccattccgaa ggtgaacgtc cccctggaac tgaagaaagc catgctttcc
      121 tacctgaaaa aacaaccgaa ttacgactcg acacgggtcc gcttcgaggt cagggatgtg
      181 tattattacg cagatacgaa cctgtatcgc tgcgaattca aggtacatat ggttgtcccg
      241 tccaggaacg tcgacacaac cggaatgatg gatggcacgg tgtcgaaaga ttttaagacc
      301 gttcatagaa cggactgatc ccggaaacag ttcccgggat cagttgcact ggcataaata
      361 actccctgac cgttaggggc gcctgactag gttgcccagg ccttctgacc cttcttcagg
      421 tcgatacagc cctcgtaacg gcccggcacc ggaacataaa cgagcgcttc ggttgcccct
      481 tcctttgaag taaaataccc ccaaagcgtc aactccttca tcatcctgaa atagtgctgc
      541 gggtcttcct ttttctttgt cttcgcatat tcctttgact ccttgtcgag atcgaccagc
      601 aggtcgtgcc gttgctcggg cgtcgagtca aggaaggcct ttccgttcct tttcctgctc
      661 gcatcatcca gcttctgcat tccgtcgagg aagacttttt ggtccggctg ggtgtagcag
      721 tccctgacga tcacagtcat aaactctccc accttcgcgt ctttggcgcc cggcgtattg
      781 gtcttcggca ggatggtctc ggcgacctcg tccaggaagg caacatcgct gtttgaaaaa
      841 ttggtgccca tgccgccgag cttctgctcc ggagggttgg tgcagcctga aaggaaagcc
      901 tccgcgccga ggatggttcc cccaagcagc aggctcacat gggttatggc atctcttctg
      961 ttcatagtat ttttttttga ggtgggttca atcagatgtt ttgttttttc agctcgctta
     1021 ccgcatgatc cgccgccctg gccgtgaatg ccatatagct caacgacggg ttgcagcaat
     1081 tcgcggaagc catgaaagat ccgtccgtga cgaacacatt cggcgcatcc caaacctggt
     1141 tccacttgtt cagcactgat gtcttcgggt cgcgtcccat cctcgccgtg cccatctcat
     1201 ggatgccccg gcccgggaag gggttgtcgc tgcggctcct gacattcttc acgccggcga
     1261 tggtcagcat ttcgacggca tcggcctcca tatccttccg catcttgagt tcattttcct
     1321 tcagctcaca gtcgatggcc aggatattca gaccccattt atcctttctt gtcttgtcca
     1381 gcgtcacctt gttctcatga taaggcagga tctcaccgaa tgcgccaata ctcattgtcc
     1441 agggtcccgg ctccgttagc gcctccttat attctttgcc gatcgaatat tccgcgactt
     1501 cgtgctggta accttgccgg ccgccgccgc cctggtaacc gaacccgcgg atataatcgc
     1561 gcttttcgcc gtacaggttg cggtatttcg gaatatagat cccgttggcg cgtctgccgt
     1621 agtagtattt gtcttcatag ccttccaccg ttccgccggc gcctacaccc aggtggtggt
     1681 ccatgaggtt gtgtcccaac tcgccactgc tgctgcccag accgtcgggc cagacgtctg
     1741 ttgctgaatt catcagtacc caggcgctgt taagcgtgct ggcattcaga aagacgatct
     1801 tagacttgaa ttcatatgtt ttgttcgtct ctgcgtccag cacttccacg cctgtggcgc
     1861 gcttcttatc cttatcataa aggatcttgg tcacgatcga ccagggcctc agcgtgagac
     1921 gtcccgtagc ccgcgctgcc ggaagcgtcg acgattgcgt gctgaaatag ccgccgaacg
     1981 gacagcccag ccagcacttg ttccggaact cgcagcgccg ggtgccaagc ggctgcgtga
     2041 gattggccgt ccgcccgatg atcatatggc gtttcccctt ata
//
LOCUS       DATGKE010000390         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1515361, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000390 DATGKE010000000
VERSION     DATGKE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515361"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..838)
                     /locus_tag="VKU83_06765"
     CDS             complement(269..838)
                     /locus_tag="VKU83_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93290.1"
                     /translation="MDIERMVGVLGALVISAAQAIYILNCVRRKITPSVLSWFGWSCL
                     MGTSLVSQIVHKGWQWSMTGIASSAVGCLTIAAVAWLSGNFAFKRGDLAFLLAGLACV
                     GIYVCSDDSWVTTIFAIVADALLGVPTIVKAWRLPDLERSVAWVLGVVSAALSLSICV
                     GHSFIYVLFPAYLLLFNGSMAGLTWGRRR"
     gene            complement(841..1821)
                     /locus_tag="VKU83_06770"
     CDS             complement(841..1821)
                     /locus_tag="VKU83_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLI93291.1"
                     /translation="MKSFVLAITMFCVWEMHGSAAPGFESPASRSDAKAAVSDTAALL
                     GRWDLTVYMNGHAVPSWFEVIKSGTRMLVGRFVGGGGSARPVSRYYFADGKLTFSIPP
                     QWEQEDRDLSVEGHFEGDSLVGTLVQPNGKQYNWVGYKAPALQRSSKPVWGAPVRIFD
                     GKTLNGWHAPGNRANQWLAENGVLRSPHSGSNIVTDGKYRDFKLHIEFRYPKGSNSGV
                     YLRGRYEVQIEDAEGTEPPNDVISAVYGFLPPNAQASKGPGEWQTYDITLVGRLVTIV
                     FNGRTVICNAEIPGITGGALDSHEGEPGPIYLQGDHGPIEFRNISIQTAP"
     gene            1876..2874
                     /locus_tag="VKU83_06775"
     CDS             1876..2874
                     /locus_tag="VKU83_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HLI93292.1"
                     /translation="MRHRMYFAVLWTLLLPSMATAQPGNRSARRTSGNPILSGWYADP
                     ETAVFDQQYWIYPTFSARYNQQVFFDAFSSSDLIHWEKHSHILDTSGVRWAHRAMWAP
                     AIAKKGGKYYFFFGANDIQNDSSAGGIGVAVADHPGGPYKDYLGHPLISAFHNGAQPI
                     DQATFQDPADGSWYLIYGGWKHCNIAKLRPDFTGFIPFADGTTFKEITHDNYVEGPYM
                     IYRKGKYYFMWSEGGWTGPDYSVAYAMADRPTGPFNRIGKILQQDMAVATGAGHHSVL
                     HIPGTDDYYIVYHRRPLGETDPNHRVVCIDKMEFDSEGHILPVRITKEGVVARPLR"
     gene            complement(2916..>3299)
                     /locus_tag="VKU83_06780"
     CDS             complement(2916..>3299)
                     /locus_tag="VKU83_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93293.1"
                     /translation="KVFMTFSVLTILITCLGLLGLIAFTTQQRQKEISIRKIMGAGIG
                     QIVPLLTKNFVLLVGISCVFAFPVAGYFMHEWLTHFSYSMGLGLLWMAFLLSALTVLL
                     IAMMTVIFHAVRAAVANPAVNLRTE"
BASE COUNT          758 a         1025 c          835 g          681 t
ORIGIN      
        1 ctggtgtacc gtaatcgtct ccccatctct tgtctggact ttgttcccga agcgaaggtc
       61 ttttgcgtgt atcatagagt ctacttttaa taccttgaaa aacaatggat taattttccg
      121 gtatttcaag ggatagactc ctttagagag atcaactgaa aggctaaaat agtaagtacg
      181 gcccacattt gcaataggct tcggcccgcg acttatccac agccttattc ggccccggtc
      241 gccgaagctc cggcgagggc gacccatgtc aacgccgccg cccccaggtc aatcccgcca
      301 tacttccatt aaaaaggagg agatacgccg gaaataaaac atatataaag ctatgaccca
      361 cgcaaatcga caaggacaac gccgccgaga ccaccccgag gacccacgct accgaccgct
      421 caaggtccgg caaccgccag gccttaacga tggtcggaac acccagtagc gcatcggcga
      481 cgatggcaaa aatggtcgtc acccaggaat cgtcggagca tacatatata cccacgcaag
      541 ccaggcctgc caggaggaat gccaggtcgc cgcgtttgaa tgcaaaattg ccgctcaacc
      601 atgctaccgc cgcaatagtc agacaaccga ccgcggagct cgcaattccc gtcatgctcc
      661 attgccagcc cttgtgcacg atctgcgaaa cgaggctcgt acccattaaa cacgaccagc
      721 caaaccagga gagcacactg ggagtaattt tccttcggac acaattcaag atatagatcg
      781 cctgggctgc gctgatcacc agcgcgccaa gaacgcccac catccgctcg atgtccatgg
      841 ctacggcgcg gtctggatgg aaatattccg gaattcgatc ggcccatgat cgccctgcag
      901 ataaatgggc cccggctcgc cttcgtgact gtcgagcgct ccgccggtaa tgcccgggat
      961 ctcggcattg cagatgacag tccggccatt gaagacaatc gtcaccagcc ttccaacgag
     1021 cgtaatgtca taggtctgcc actcccctgg tcctttcgag gcctgggcat tgggcggaag
     1081 gaatccgtag acagcgctaa tgacgtcatt cggcggctcg gttccttctg catcttcgat
     1141 ctggacctca tatctccccc gaaggtacac gccgctattg ctgcccttcg gatacctgaa
     1201 ttcaatgtga agtttgaagt cccggtattt tccatcagta acgatgtttg agcccgagtg
     1261 cgggctccgc aggacgccgt tctccgcgag ccactgattg gcgcggtttc cgggcgcatg
     1321 ccagccgttt agcgtcttgc cgtcgaaaat gcgcaccggc gcaccccaca caggcttgga
     1381 gctgcgctgc aaggccggcg ctttatagcc aacccaattg tattgcttac cgtttggctg
     1441 gaccagcgtc ccgaccagac tgtccccttc aaaatgaccc tcgacggaaa ggtcgcgatc
     1501 ctcctgttcc cattgcggcg gaatggaaaa cgtcaatttc ccatccgcaa aataatagcg
     1561 cgacaccggc cgtgcgcttc cgccgccgcc tacaaaccgg ccaaccagca ttctcgtccc
     1621 cgacttgatc acctcgaacc aggaaggtac cgcgtgtccg ttcatgtaaa ccgtaaggtc
     1681 ccaacgcccc agcaaggccg ccgtatcgga cacggctgcc ttcgcatccg accgtgaagc
     1741 cggtgattcg aacccgggcg ccgccgatcc gtgcatttcc catacgcaaa acattgtaat
     1801 cgccagaaca aaacttttca ttcgtaaaaa atttgccggg aaggtacagt ttttttttcc
     1861 gtagattggc cgaatatgcg acaccggatg tattttgccg tcctctggac gctgctgctg
     1921 ccgtcgatgg cgacggccca gccgggcaac cgttccgccc gccggacctc gggcaacccc
     1981 attctttccg gctggtatgc tgaccctgag accgcggttt ttgatcagca atattggatc
     2041 taccccacgt ttagcgcgcg ctataaccag caagtctttt tcgacgcctt ctcttcatcg
     2101 gacctcatcc attgggaaaa gcactcccat atcctcgata cctccggggt gcggtgggct
     2161 caccgcgcca tgtgggcgcc cgcgattgca aagaaaggcg gaaaatatta ttttttcttt
     2221 ggggcaaacg atattcagaa cgattcttcg gcgggtggga tcggcgtcgc ggtagccgac
     2281 caccctggcg gcccctataa agattatctc ggtcatccgc tcatctctgc cttccacaac
     2341 ggcgcccaac cgatcgacca ggcgacgttc caggatcccg ccgacggcag ctggtacctc
     2401 atttacgggg gatggaagca ttgcaacatc gcgaagctgc gtcccgactt cacaggcttc
     2461 attccgttcg ccgatggtac gaccttcaaa gagatcacac acgacaacta cgtcgaaggc
     2521 ccgtacatga tctatcgcaa ggggaaatac tatttcatgt ggtcggaagg cggctggacg
     2581 ggtccggatt attccgtcgc ctacgctatg gccgaccggc ccaccggccc ttttaaccgg
     2641 atcggcaaga tcctccagca ggatatggcc gtcgccaccg gagcaggcca ccattccgtt
     2701 ctgcacatcc ccggcaccga cgattattat attgtctatc accggcggcc cctcggcgaa
     2761 acggatccga accatcgcgt cgtctgcatt gataaaatgg aatttgatag cgaggggcat
     2821 atcctccctg tcagaattac gaaggaaggc gtggtcgcga ggcctttacg ttaagaatac
     2881 gcgccagcgg ccgcccccgc ctggcctggc cgcccctatt ccgtccggag gttcaccgcc
     2941 ggattcgcca ccgcggccct cactgcgtgg aagataaccg tcatcatagc gatcagcaag
     3001 accgtcaggg ccgacagcaa aaaagccatc cacagcaggc ctagccccat gctataggaa
     3061 aagtgcgtca gccactcgtg catgaaatac ccggccaccg gaaacgcaaa aacgcaggat
     3121 atcccgacca gcagcacgaa attctttgtc agcaagggca caatctgccc gatgccggcg
     3181 cccatgatct tccggatgct aatctctttc tggcgttgct gtgtggtgaa agcgattaat
     3241 ccaagcagac ccaggcaggt gatcaggata gtcaggacgg agaatgtcat gaaaacctt
//
LOCUS       DATGKE010000391         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_936840, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000391 DATGKE010000000
VERSION     DATGKE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936840"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VKU83_06785"
     CDS             <1..272
                     /locus_tag="VKU83_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HLI93294.1"
                     /translation="RKDSLAIYQEQKRPDLARKEEEEIRVIERFLPRQLEGDALKEAV
                     SGIIAESGATGPADIGKVMGLATKRLAGQADGKSISAVAKELLSR"
     gene            272..802
                     /locus_tag="VKU83_06790"
     CDS             272..802
                     /locus_tag="VKU83_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HLI93295.1"
                     /translation="MTIDVLFLLAMVLAIFRGMRHGFIISVLSAAAIFIGLAAAIRLS
                     ASVAASSSAGIAHPSRWLPVLAFLLIFLVVVIVVRLLARMLEKAVDLAMMGWLNKLAG
                     VLLYAVIYAIILSVLLFYATQLHLVGTKTLRSSVTYPFIRPWGPVVIDGFGKFVPWFE
                     GMFSRLQDFFGRFDSH"
     gene            914..1690
                     /locus_tag="VKU83_06795"
     CDS             914..1690
                     /locus_tag="VKU83_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI93296.1"
                     /translation="MEYEIKQDDKFRFIEEGQGEPLLLLHGLFGALSNFRGQIDYFRK
                     DYKVVVPILPLLDLDLIHTTVGGLEKFVNKFVEHRDYTHINLLGNSLGGHVALMHVLR
                     HPERIRSLTLTGSSGLFENGMGDTYPKRKDYEYIRKKTELTFYDPQMATKELVDELYE
                     TVNVRLKAIKIIALAKNAIRNNLGEEVNQIKVPTLLIWGKNDAITPPFVGQEFHKLIP
                     NSELHFIDKCGHAPMMERPDEFNTILLKFLRKLNEPAAVA"
     gene            1726..2388
                     /locus_tag="VKU83_06800"
     CDS             1726..2388
                     /locus_tag="VKU83_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLI93297.1"
                     /translation="MLNKEIISSAVPSLHFDDPVSQALDLMADFHVTHLPVVAGDKLV
                     GLVSEEHLLNVEDDNVLLSQLQPAFSGIAAHADAYFFESVQLVNEHSLTLIPVVAGES
                     EYTGSIIATDLLRHLGRVTGVKDPGGIIVLEMEKLSFSFTEISKLVETNDAQITQLNT
                     YPDLTSGNFLISLRINKAEISDIVATFQRYEYQVKYYFGEELYENELRNNYDHLMNYL
                     NI"
     gene            2388..3266
                     /locus_tag="VKU83_06805"
     CDS             2388..3266
                     /locus_tag="VKU83_06805"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HLI93298.1"
                     /translation="MKVAIYSRVIEYEQQSGVQRLFDELIRQHLQPVIHQPFLEKIGS
                     AFVLPADIGSFRESDDLDESIEFLISLGGDGTLLDTVSLVRDKNIPVLGINFGRLGFL
                     ASIGKEELTSAVTALANRTFVVDKRSLIHLDANKPLFGEVPYGLNEFAIHKTDTSPMI
                     KIHTYLNGEFLNTYWADGLIVATPTGSTGYSLSCGGPVVFPESASFVITPVAPHNLNV
                     RPIVVPDDNIISFEVEGRSENFICVLDSRKEIVDKQVQLAVRKESFTLSLIRLNENNF
                     LQTLRGKLSWGLDTRN"
     gene            3413..4273
                     /locus_tag="VKU83_06810"
     CDS             3413..4273
                     /locus_tag="VKU83_06810"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HLI93299.1"
                     /translation="MVKKLIFCALLAGWFGFAASAQEQAFVHEGEYGFNIGASQYFGD
                     LNPNPRFNTPNIAFGAFFRKQFGNYVALRVAANYTFLGYSDRYNTRNEFMYRRNLSFN
                     TNIYELGLQGDFNFFKFVPGSQYNRFTPYITFGIGAFYYNPYTYYQGQKVYLRTLGTE
                     GQGSAAYPGRKEYSTMAVCFPIGVGLKYNVNRRMNIGVEALYRFTTTDYIDDVSSTYA
                     PNAQPHYLPNGQPTLWYALQDRSYETGEPIGIQGRQRGYSNQKDSWLTAVVFVSWNIM
                     GYRCPTANPL"
     gene            4395..>4966
                     /gene="uppS"
                     /locus_tag="VKU83_06815"
     CDS             4395..>4966
                     /gene="uppS"
                     /locus_tag="VKU83_06815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HLI93300.1"
                     /translation="MGRLPSHIAIIMDGNGRWAQEKGQDRLFGHFHGVESVRDIVEGC
                     AELGIGYLTLYAFSTENWDRPAKEVTGLMELLVETIRNEIDTLNRNNIRLHVIGDMSM
                     LPDYARAELNEALQLTAANRGLNLVMALSYSSRWELTEAVKSLAADVRSGKLDPAAIT
                     QDTLQQYLSTRDFPDPELMIRTSGEYRISN"
BASE COUNT         1149 a         1279 c         1386 g         1152 t
ORIGIN      
        1 agcggaagga ttcgctggcc atttaccagg agcagaagcg gcccgacctc gccaggaaag
       61 aggaagagga aatccgggtg atcgaacgat ttcttccccg gcagctggag ggggatgcat
      121 tgaaagaagc ggtgtcggga attattgctg agtcgggtgc cacgggaccg gccgatatcg
      181 ggaaagtgat gggcctggcg acaaagaggc tggccgggca ggccgatggg aaatcgatca
      241 gcgccgtggc aaaagagtta ttgtcccggt aatgaccatc gatgtccttt tcctgctggc
      301 gatggtgctt gccatattca ggggaatgag gcacggattc atcatttccg tcctttccgc
      361 ggccgcgatc ttcataggac tggcggcggc gatccggttg tcggcatccg ttgccgcctc
      421 ttcttcggcc ggtatagctc atccatcgcg gtggctgccc gtgctggcct tcctgctgat
      481 cttcctcgtg gtcgtcatcg tggtgcggct gctggccagg atgctcgaga aggcggtcga
      541 cctggcgatg atgggttggc tgaataagct ggctggcgtc ctgttatatg ccgtcatcta
      601 tgccatcata ctaagcgtgt tgcttttcta tgcaacacaa ctgcatcttg tcggaacaaa
      661 aacgctcagg tcgtctgtca cctatccctt tatccgcccc tggggcccgg tggtaatcga
      721 tggattcggt aaatttgtac cgtggttcga gggaatgttc agccgcctcc aggacttttt
      781 tggccggttc gacagccatt gaggcgggga taaggtaagc tgttttaatg cgatcagcga
      841 gtgttttttt gcggtaaatc gtttatttta gcggttaaat atggtcattt aagggataga
      901 atcttaatag ctgatggaat acgaaatcaa acaggacgat aagttcagat tcattgagga
      961 aggtcaaggc gagccgctgt tgttattaca cgggttgttt ggcgcgttga gtaatttccg
     1021 cggccagatc gattattttc ggaaggatta caaggtagtg gtcccgatat tgcctttatt
     1081 ggacctggat ctcatccaca cgacggtagg ggggctggag aaattcgtta ataagttcgt
     1141 cgaacaccgc gattataccc atatcaacct gttaggcaac tcgctcggcg gccatgtcgc
     1201 cctgatgcat gtattgagac atcccgagcg gatccggtcg ctgaccctca cgggaagctc
     1261 cgggcttttc gagaacggga tgggagatac atacccgaag cggaaggact acgagtatat
     1321 ccggaaaaag actgaattga ccttctatga ccctcaaatg gccacaaaag agctggtgga
     1381 cgaattgtat gagaccgtga atgtccgcct gaaggctatc aagatcattg cgcttgccaa
     1441 gaacgccatc cgtaacaatc tcggggaaga ggttaaccag atcaaggtgc ctacgctgct
     1501 gatctggggt aagaatgatg ccattacgcc gcccttcgtc ggccaggaat tccataagct
     1561 gatccctaat agcgaactgc attttatcga caaatgcggt catgcgccga tgatggagcg
     1621 gccggacgag ttcaacacca tattgcttaa atttttgcga aaattgaacg aaccggcagc
     1681 ggtagcttga tttattgcgt ctaaaatgta ataatactgt cgcaattgct aaacaaagag
     1741 atcatatcct cggccgtgcc gtcactgcat tttgatgatc ccgtgtccca ggcgctggat
     1801 ctcatggcag atttccatgt cacgcacctg ccggttgttg cgggcgacaa gctcgtgggc
     1861 cttgtcagcg aggagcacct ccttaacgtg gaggatgaca atgtcctgtt gtcgcagctg
     1921 caacctgctt tttccgggat agccgcgcac gcggacgctt atttcttcga atccgtgcag
     1981 ctggtcaatg agcacagtct gacgcttatc ccggtcgtgg cgggggaatc cgaatacacc
     2041 ggctcgatca tcgcaacgga cctgttgcgg caccttggcc gggttaccgg cgtcaaagac
     2101 ccgggcggga taatagtcct cgagatggaa aaacttagtt tctcattcac ggagatcagc
     2161 aagcttgttg agaccaatga cgcccagatc acgcagctga acacataccc tgacctgact
     2221 tccggtaatt tcctgatcag cctgcgcatc aacaaagccg agatctccga tatcgtggcg
     2281 acctttcaac ggtacgaata ccaggtcaaa tactattttg gcgaggagct gtatgaaaat
     2341 gaattgcgta acaattatga ccatctgatg aattatctca acatctgatg aaggtagcaa
     2401 tctacagccg ggttatcgaa tacgagcagc agagcggcgt tcaacggtta tttgacgagc
     2461 tgatccggca acatctccag ccggtgatcc accagccctt tctcgaaaag ataggttccg
     2521 cgtttgtact ccccgccgat atcggcagct tccgcgagtc ggatgacctc gacgagtcga
     2581 tcgagtttct catcagcctg ggcggcgatg gaacgttgct ggataccgtt tccctggtgc
     2641 gcgacaagaa tatccccgtt ctcggcatca atttcggccg gcttggattt ctggcgagca
     2701 tcggcaagga agagctgact tcggcggtga ccgccttagc gaaccggact tttgtggtgg
     2761 acaagcgctc attgatccac ctggatgcca acaaacctct ttttggggag gtcccgtacg
     2821 gcttgaatga gtttgcgatc cacaagaccg acacctcgcc gatgatcaag atccacacgt
     2881 atctgaatgg tgagttcctc aatacatact gggccgacgg gctgatcgtt gcgacgccta
     2941 ccggttccac cggctattcg ctgagctgcg gcgggccggt agtcttcccg gagtcggcca
     3001 gtttcgtgat cacgccggtg gcgccgcaca acctcaacgt gcggccgatc gtcgtgccgg
     3061 acgacaatat catctctttc gaggtcgaag gccggtccga gaatttcatt tgcgtcctgg
     3121 actcgagaaa ggagatcgtg gacaagcagg tgcagctcgc agttaggaaa gagtccttta
     3181 cgctgagcct cattcgcctg aacgagaaca attttcttca gaccctgcgc ggcaaacttt
     3241 catggggatt ggatacgcgg aattaaggca ttgggtggtg gacaagccgg ccggccccgc
     3301 tttccggcct ttggcacgaa aagtgccggt aaacttttaa catttgaatg ggattcccga
     3361 caaacgaaaa accggtgaaa ttcgttcttg gggttagata ttttcataca tcatggtgaa
     3421 aaaactaatt ttctgcgcct tgctcgccgg ctggtttgga ttcgcggcat cggcgcagga
     3481 acaggcattt gttcacgaag gggaatacgg tttcaacatc ggggcctcgc aatatttcgg
     3541 ggatctgaat cccaatccgc ggtttaatac cccgaatatc gcgtttggcg ccttttttcg
     3601 aaagcaattc gggaattatg tggcactccg ggtagccgcc aactatacat tcctggggta
     3661 ttcggaccgg tataacacca ggaacgaatt catgtaccgc cggaatctga gcttcaatac
     3721 caacatttat gagctcggac tgcagggcga cttcaacttc ttcaagttcg ttcccggtag
     3781 ccagtataat cgttttactc cttatatcac gtttgggatc ggcgccttct attataatcc
     3841 ctatacctat taccagggcc agaaagtata tctgcggacg cttgggacgg agggacaggg
     3901 ctcggcggcg taccccggcc ggaaggaata cagtaccatg gcggtctgct ttcccatcgg
     3961 cgtcggcctg aaatacaatg tcaaccgcag gatgaacatc ggggtcgagg cgctctaccg
     4021 gttcacaacg acagactata tcgacgacgt cagcagcact tacgctccca acgcgcagcc
     4081 ccattacctg ccgaacggac agcccacgtt gtggtatgca ctgcaggacc gctcctacga
     4141 gacaggcgaa ccgatcggca ttcagggtcg ccagcgggga tactccaacc aaaaagacag
     4201 ctggctgacg gcggttgttt ttgtctcctg gaatataatg ggctaccggt gccccaccgc
     4261 gaacccgctc taaacgggac aatagggcaa aatctcctat atttgtgccc cctgaagccg
     4321 ccggtttgca ggacggcagg acgagctttt aaacaaacaa cctggttatg gatgggttgc
     4381 gggaaaaaat agacatggga agactgccca gccatattgc catcattatg gatggaaatg
     4441 gccgctgggc ccaggaaaaa ggccaggatc gccttttcgg ccattttcac ggcgtggaga
     4501 gcgtccgcga cattgtcgaa ggttgcgctg aactcggcat cgggtatctg accctgtatg
     4561 ctttttcgac cgagaactgg gaccgccccg ccaaggaagt gaccggactg atggagctgc
     4621 tggtcgaaac gatcaggaat gaaatagaca cgctgaaccg gaacaatatc cggctgcacg
     4681 tgatcggcga catgagcatg ttgcccgatt atgcccgggc ggaattgaat gaggcgctgc
     4741 aactgacggc cgccaaccgc ggccttaacc tggtgatggc cctcagctac agtagccgct
     4801 gggagctgac ggaagccgtc aaaagccttg cagcggatgt ccgttcggga aagctggatc
     4861 ccgcggccat cacccaggat actttgcagc aatacctgtc gacccgggat tttccggatc
     4921 cggagctgat gatccggacg agcggcgaat accggatcag caattt
//
LOCUS       DATGKE010000392         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1180752, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000392 DATGKE010000000
VERSION     DATGKE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180752"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VKU83_06820"
     CDS             complement(<1..1876)
                     /locus_tag="VKU83_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI93301.1"
                     /translation="MDRTIYNVERDMSLAGGTAIDALRRVPILSVDIDGNVTLRGSAN
                     IIILINGKPSTITANNLADALKQIPVDEIKSVEVITSPSAKYDADGSAGMINIVLRQD
                     RLHGVLLNPDMAFGTRASFLGINGGYNNKNMSFSVGGFGRVSYNVNGIYANLQTVGSS
                     DISQQAATRKADLADNYHAGWEYDMDKFNFLTASLRYSQSNNHLHQDNLLSSFYTGSG
                     PDSLQLNQVATTNRSATVDATIDYTHAFKNPQREFSLLTLFSRTAGSSAFTNIQQNPS
                     DDALINGKTNSDASSNQEVTLQADYQTPVDSNQLLEFGGKYIIRGVVSNYNYLSAMGS
                     NPPVTETSPYLTNRFSYHQNVTAGYLEYTLNTRLPLSFRVGARYEYTAISAGLLDAGV
                     TLPSIRSYGVFVPAVNLGWRLDNGRIITWGFSRRIQRPSIQYLNPNLVAADPGNISTG
                     NPALGPEYSNNFELGYNTAIGSLAVGFSGFYRQTTNSIASVSLPTAGGDTIERTYANV
                     GRESTGGMNLFANLDAGQNLSLSGGLNIYYTELKNAGLSQGAGNPGPAGRNSGWILSA
                     NLSGGYNFAKKWSLYLFSYYRGSQVLLQGYQTGFPYYSLTLKRSLPKKLGTVGIGAEN
                     F"
BASE COUNT          461 a          525 c          535 g          504 t
ORIGIN      
        1 aaaaattttc cgctccaatg ccgaccgtgc caagtttttt cggcaagctt cgttttaagg
       61 ttaaactgta ataagggaag ccggtctgat aaccctgcaa cagaacctga cttccgcggt
      121 aatagctgaa taaatagagc gaccattttt tggcaaaatt gtagccgccg gataggttgg
      181 cgctaagaat ccaaccggaa ttccgaccgg cggggccggg attacctgct ccttgcgaga
      241 ggccggcatt tttcagttcg gtgtaataaa tgttcagccc gccgctcaag gacaggttct
      301 gtcccgcatc cagattggcg aaaagattca tgccccccgt tgactccctg ccgacatttg
      361 cgtaggtgcg ctctatggta tcgccgccag cggtaggcag gctcacgctt gcaatcgaat
      421 ttgtcgtttg gcgataaaat ccggaaaacc cgaccgccaa ggacccgatt gcggtgttgt
      481 accctagctc gaagttgttc gaatattccg ggccgagcgc cggatttccg gtggagatgt
      541 tcccgggatc tgcagccacc aggtttggat tgaggtactg gatggaaggg cgttgaatgc
      601 ggcggctaaa gccccatgta atgattcttc cgttgtccag ccgccacccc aggttaacgg
      661 ccggaacaaa aaccccatag gaccgaatgg acggcagcgt gacgcctgcg tccagcaatc
      721 cagcgctaat ggcggtgtat tcataccggg cgcccacgcg aaaactgagt ggcaaccggg
      781 tattcagtgt atactccagg tagcccgccg taacattttg gtggtaggaa aagcgattgg
      841 taagataagg tgaagtttcc gtaacgggcg ggttgcttcc catggcggat aaataattgt
      901 agttgctaac gacgccccgg atgatatact tgccgccgaa ttcaagcaac tggttggagt
      961 cgaccggtgt ttggtagtca gcctgtagcg tgacctcctg gttcgaactc gcatcactat
     1021 tcgtcttccc attgatcaag gcgtcgtccg aggggttttg ctgaatgttg gtaaatgcgc
     1081 tgctcccagc cgtccggcta aacagggtaa ggaggctaaa ctcccgctgc gggtttttaa
     1141 aagcgtgggt ataatctatc gttgcgtcga ccgttgcaga cctgttcgtt gttgcaacct
     1201 ggttcaactg cagactgtcc ggtccggatc cggtgtaaaa actgcttagc aggttgtcct
     1261 ggtggagatg attattggat tgactatatc gcagggatgc ggtcaaaaag ttgaatttgt
     1321 ccatatcgta ttcccatccc gcatggtaat tgtccgccag gtcggctttt cgggtggccg
     1381 cctgctggga gatgtcactc gatcctacgg tttgcaggtt tgcgtatatg ccattgacat
     1441 tgtagctcac ccggccaaag ccgccaaccg aaaaggacat atttttattg ttgtagcccc
     1501 cgttaatacc caaaaatgaa gctctggtgc caaatgccat atccgggttt aacaagaccc
     1561 cgtgtaaccg gtcctggcgc aaaacgatgt tgatcattcc ggccgaaccg tccgcgtcgt
     1621 atttcgctga aggggaagtg atcacttcta cagacttgat ttcatcgacg ggtatttgct
     1681 tcagggcatc cgccagattg tttgcggtga tcgtcgacgg cttaccattg atcaggatta
     1741 taatattggc gcttccccgc agcgtcacat tcccgtcaat atccaccgac aggatcggca
     1801 cccgtcgcaa tgcatcgatt gcagtcccgc cagcaagcga catatcgcgc tccacgttat
     1861 aaatagttcg atccaatttg tcctcgatta aaacgcgtct gctgtcaatg accacctcct
     1921 tcatcatctc cgaacggtgg ctgaggaaaa gcgtgccaag gtttaacgac tcatttgccg
     1981 ctattaacct caccggtatt cggacgggat gatacccaac cgatg
//
LOCUS       DATGKE010000393         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_338299, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000393 DATGKE010000000
VERSION     DATGKE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338299"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VKU83_06825"
     CDS             complement(<1..475)
                     /locus_tag="VKU83_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93302.1"
                     /translation="MKALLFLCCCFGAFVSRAQQRQHRSLDQDWRFHFGDAADPAKDF
                     NYSQVSIFSKSGKAEGTAIAPKFDDRDWRHVSVPHDWAVELPFEYSKDFDVMAHGYKP
                     VGGFYPATSIGWYRKRFEVARTDSGQRFVLQFDGVFRDCKVWLNGFYCCSNASGYS"
     gene            complement(504..1463)
                     /locus_tag="VKU83_06830"
     CDS             complement(504..1463)
                     /locus_tag="VKU83_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HLI93303.1"
                     /translation="MDTTTTTPKDTPILKGADVSWLTQMEDSGRLFYDSAGTQMDCMQ
                     LLKGLGINLIRLRAWVHPATKYNSTGDVVAKAVRAHSLGLRVMIDFHYSDTWADPGHQ
                     TKPATWAGASLAALGDSVATYTASVLDSLKSAGVVPGYVQLGNETDNGMLWPDGEADS
                     SMANYAALVNSAYNAVKAVSDTIKVIVHLSNGYDSTHFQWLFDGLKANGASWDVIGMS
                     LYPSATDWQTYNQECLANMNALVTRYNKPVMVCEVGMPENQAGTCEEFITDLIHKVRA
                     VNGGEGLGVLYWEPECYNNWQGYGLGAFDATGKPTVAMDAFLN"
     gene            complement(1627..2097)
                     /locus_tag="VKU83_06835"
     CDS             complement(1627..2097)
                     /locus_tag="VKU83_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93304.1"
                     /translation="MEIDHSSQVGDEMDYTTNSPFVKSLMTGLAIGVSDTILSLLFNL
                     FYRHDATSGYFSSDYVSVSSIIFGINILFVVIGLLFAAVQGGGRLAEWVFSLVFLVLT
                     IVGVIAAMGVHPWADPLQNNRFHGLLAGLVIIVGVSAALLPVIYHNRKFRQYVL"
     gene            complement(2145..>3193)
                     /locus_tag="VKU83_06840"
     CDS             complement(2145..>3193)
                     /locus_tag="VKU83_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLI93305.1"
                     /translation="RPNLFLPATPGAAGALIDLDGRWGLHPAMRAFEPMFREGRLAIV
                     HGMGSPNNTRSHFDAQDYMESGTPFDKGTSSGWLNRAVGLLGHDALTPFTAVSLTSAL
                     PRSFYGDNPAMAISTLEEFRIGLKDDAAANAAANELENLYRRTAPGLLQQTAKESFEA
                     MKVLQSAESRNYRPANGTSYPNSSLGRALRQIAQLVKMDVGLEIAFTECGGWDTHFNQ
                     GTDNGIFARSATDLSNSIAAFWRDIDAWQDDVTVMTMTEFGRTVHQNGTGGTDHGRAS
                     CNFILGNAVAGGIVHGKVAELAVENLEDGRDLAVTTDFRSVFAAVADAHLQIANDRVL
                     FPGWDADKAKLMKL"
BASE COUNT          715 a          974 c          810 g          694 t
ORIGIN      
        1 cgctatagcc gctcgcattc gagcagcaat aaaatccgtt cagccagacc ttgcagtccc
       61 tgaaaacgcc gtcgaattgc agcacgaagc gctggcctga atccgtccgg gcgacctcga
      121 agcgcttgcg ataccaacca atgctcgtgg ccggataaaa gccgccaaca ggcttgtagc
      181 cgtgcgccat cacgtcaaag tccttcgaat actcgaatgg caattcgacg gcccagtcgt
      241 gcggaacaga gacgtggcgc cagtccctgt catcaaattt cggcgcgatc gccgtacctt
      301 cagccttgcc cgatttggaa aaaatgctga cctgactata attgaaatct ttggccggat
      361 ccgcggcatc gccaaaatga aaccgccaat cctgatccaa cgaacgatgc tgccgctgct
      421 gggcgcgtga aacaaaggcg ccaaaacagc agcataaaaa aagaagtgct ttcacttaga
      481 ccttattaat tgctttgtga agattaattt aaaaaagcat ccattgcgac tgtcggtttg
      541 ccggtggcgt caaaggcccc gagtccatat ccctgccagt tattataaca ttccggttcc
      601 cagtacaata cgccgagccc ttcgccgcca ttgaccgccc ggaccttatg gatgaggtcg
      661 gtgatgaact cctcgcaggt tccggcctgg ttttcgggca ttcccacctc gcagaccatg
      721 accggcttgt tataccgcgt caccagggcg ttcatgttgg ccaggcattc ctggttatag
      781 gtttgccagt ccgtagctga aggatacaaa gacatgccga taacatccca ggaagcaccg
      841 ttcgccttca ggccgtcaaa caaccattgg aaatgtgtgc tgtcatatcc attcgataaa
      901 tgtacaatga cctttatcgt gtcgctcacg gccttcaccg cattataggc cgagttgacc
      961 agcgccgcat agttcgccat gctgctgtca gcctcgccgt cgggccacag catgccgtta
     1021 tcggtctcat tgcccagctg cacatagccc ggcaccactc cagccgattt cagcgagtcg
     1081 aggacagatg ccgtataggt ggcgacactg tcgcccagtg cggctaaact tgccccggcc
     1141 catgtcgccg gcttcgtctg atgaccggga tcggcccagg tgtcgctgta atgaaaatcg
     1201 atcattaccc tcaagcccag gctgtgagcg cggactgctt tcgcaaccac atcgcccgtg
     1261 ctgttgtatt tcgttgccgg atggacccat gcgcgcagcc ggatgaggtt aatgcccaag
     1321 ccctttagca actgcataca gtccatctgt gttccggcag aatcataaaa taatcgcccg
     1381 gagtcttcca tctgcgtgag ccagctcaca tccgcgcctt tcaatatcgg agtatctttc
     1441 ggagttgtag tcgtcgtgtc caacgggggt gagtgtgttc cgcccttttt gcaccggcaa
     1501 aagccgagga cgcaaacagc tactagccct atcatcattt tcattcccca aaggaactaa
     1561 aaaaaacgct ccgggccgct tcgcagcccg gagccgaaat aacatatttt cagttgtaat
     1621 atttagctac aacacatact gccgaaactt tctgttgtga tagataacag gcaacagcgc
     1681 tgcactcacg ccgacaatga ttaccaggcc ggcgagtagc ccgtgaaacc ggttgttttg
     1741 taatggatcg gcccagggat gcactcccat tgccgcgatc accccgacga tcgtcagcac
     1801 aaggaagaca aggctgaaca cccattcggc cagccggccg ccgccctgca ccgcggcaaa
     1861 aaggagcccg atgaccacaa agagaatatt gataccgaaa atgatggaag acacgctcac
     1921 ataatccgaa gagaaatagc cgctcgtggc atcatgccgg taaaacaggt tgaacagcag
     1981 agacaggatc gtgtccgata caccgatggc aaggcctgtc atcagggatt tgacaaaggg
     2041 cgagttggtg gtataatcca tctcgtcccc tacctggctt gaatgatcga tttccatgat
     2101 tttttctttt gctacaattt acagaaaaaa tcatgcctca ccgattataa tttcatcagc
     2161 ttcgccttat ccgcgtccca gccgggaaag agcacccggt cgtttgcaat ctgcaaatgc
     2221 gcgtcggcga ccgcggcgaa aacgcttctg aaatcggtcg tgacagccag gtcccttccg
     2281 tcctccaggt tctccaccgc aagctccgcc accttcccgt ggacgatgcc gcccgctacc
     2341 gcattgccga ggataaagtt gcaggaggcc cgtccatggt ccgtcccacc cgtcccgttc
     2401 tggtgtacgg tccttccgaa ttccgtcatc gtcataaccg taacgtcgtc ctgccaggca
     2461 tcaatgtccc gccagaatgc cgcgatgctg ttgctgagat ccgtcgcgct ccgggcaaaa
     2521 atgccgttgt ccgtgccctg gttgaaatgc gtatcccagc cgccgcactc tgtaaatgcg
     2581 atctccaggc cgacatccat cttgaccagc tgcgcgatct gccgcaaggc cctgccgagg
     2641 gaggagttcg ggtagctggt accgtttgcc ggcctgtaat tcctgctctc cgccgactga
     2701 agcaccttca ttgcttcaaa gctttctttt gccgtctgtt gcagcaagcc cggcgccgtc
     2761 cggcgataaa ggttttcaag ctcattcgcc gccgcattcg cggcggcgtc atccttcagc
     2821 ccgatcctaa attcctcgag agtgctgatc gccattgccg ggttatcccc gtagaatgat
     2881 ctcggcaacg cggaggtcag gctcaccgcc gtgaaggggg tcagcgcatc atgtcccagc
     2941 aatcccacgg cccggttcag ccagccgctg gacgtccctt tatcgaaggg cgtccccgac
     3001 tccatatagt cctgtgcatc gaaatgcgag cgcgtgttgt tgggcgatcc cataccgtgg
     3061 acgatagcca gccggccctc ccggaacatg ggctcgaaag ctctcatcgc cggatgcagc
     3121 ccccaccggc cgtcgagatc gatcagtgcc ccggcggctc ccggcgttgc aggcaggaac
     3181 aggttgggcc tag
//
LOCUS       DATGKE010000394         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_67848, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000394 DATGKE010000000
VERSION     DATGKE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67848"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VKU83_06845"
     CDS             complement(<1..1211)
                     /locus_tag="VKU83_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI93306.1"
                     /translation="MSTKKLSYYLGLTAAFLLAGAVDSCTKLNQTVYSVVPNSNFWQT
                     PAQINAGVAAAYNTLTGIIPGSGWGELVEASTDEMIIPIRGADWLDGDEHIQEWQHTW
                     SSQHPNVNSAWSSIASGIGQCNFVLSVVNSLPTPPSNLSQINAQLKTLRDFYYWMAID
                     AFGDFPYITNYNTNPNTVSQTPRKQIYDSITADLLANAPLLDTRSIDSTYGKITKWFD
                     YSLLARLYLNAEVYTNTTATPGTPGTEKAYAQCVSICDSIINSGIYSLATGYFDNFSP
                     NNNTLLSGGENIYCVPFNKVSIGGQNWEMETLHYQNQTNFSLSGQPWNGFCSTADFYY
                     TFDTSSTYSSVAGTIYRTFQDQRTGQYLVGQQYSAQYNYPPDKNVIVSTTNPNISPLQ
                     DLQFKIPLVFSP"
     gene            complement(1238..>3812)
                     /locus_tag="VKU83_06850"
     CDS             complement(1238..>3812)
                     /locus_tag="VKU83_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLI93307.1"
                     /translation="LVITQQGGDPNQNPIIHLRGQTSLSGAQTPLIVVDGVTLDDPTI
                     INTIPPGDIESYSILKDASAAAIYGSRGANGVIQIITKKGHAGRSQIDYNGAVGFDKP
                     AKYYPLANVSQWEAASKQVGIADSTIQQYNQGASTNWNKAITQTAATTSQNVGMSGGT
                     GNFNYRASVDYTNNNGIVLNSGRQEIGLRFNAEEKAINDRLDIQVGILNSQVQRKYTD
                     YGVFDYIFSAPPTYPVYNADGTYHTFVDFEEANPVYHLNAEINKGQEHFTQMYGTVNF
                     EVIKGLKLGTTGSLSYYSLAKQFFQPTFATDGFSEGNVPSANQNSIYNNSGKGDIHAN
                     YLGTWGDHSLTVTAVYEYNNFSEGGFYAGAQQFIVPGLTYNNLGAGNPSYNVVSSYAD
                     RYLLISYLGRVTYSYQNKYLLTASLRRDGSSKFGANNRYGTFPSFDLGWRISQEDFMK
                     NIGWINELKIRGGYGVTGNSDNIYPYSNLFLIGSTGTAYFDAANKAVFPYPPVYSITQ
                     NANPDLKWEQRAGTNIGLDFTLFNNRLTGSFNWYNDKTTDLLYDYTVPTPPFFINTIL
                     ANVGSLTNKGVEFQFSFDAVREKDFSWTVGGNISTVNTRVTDLSGTYAGGIKLSTDYI
                     AGGYAEGRGLSSNPITYLKVGYAPYVFQLPKYEGVDKNGNQLFDSAGIKSVGVNGNPT
                     LYYYNPTAHFFYGINNTFTYKNWGLNFFMRGVQGQKIFNNTALDVAFLKRLPGNNVFT
                     SAMSNGILDQPVASTKWLENAGYLRMDNITLSYNIPHVPGFQNFKVYVTAVNLFVITK
                     YSGLDPEVRTASYQNPGNSNNPFNQSINQEAYIDANYGADGYYPRARSFSFGVNVTLN
                     K"
BASE COUNT          887 a          887 c         1029 g         1009 t
ORIGIN      
        1 ggactaaata ccaatggaat tttgaactga aggtcttgaa gcgggctgat attcggattg
       61 gtagtcgaca caataacatt tttgtcgggg gggtagttgt actgggcgct atactgttgt
      121 ccgacgaggt attgcccggt acgctgatcc tgaaaggtgc gatatatcgt tccggcaaca
      181 gaagaatagg tggaactggt atcaaaagtg taataaaaat ccgcagtgct acagaagccg
      241 ttccagggtt gaccggacag gctgaagttc gtctggttct ggtaatgaag cgtttccatt
      301 tcccagtttt gaccaccgat gctcactttg ttaaatggaa cgcaatagat gttttctcct
      361 ccagacagca gcgtgttgtt gttggggctg aaattgtcaa aatagcctgt tgccaggctg
      421 taaatgccgg aattgatgat ggagtcgcaa atgctcacgc attgtgcata cgctttttcc
      481 gtaccgggtg ttccgggagt tgcagttgtg ttggtgtaca cttccgcatt caggtaaagc
      541 cgtgcaagaa gtgaatagtc gaaccatttg gtgatcttgc catatgttga atcgatcgat
      601 cgggtatcca gcaacggcgc atttgccaac aaatccgcgg tgatcgaatc gtagatctgc
      661 ttccttggcg tctgggaaac cgtgttggga ttggtattgt aattcgtgat ataggggaaa
      721 tcgccaaatg catcgatggc catccagtaa tagaagtccc tgagcgtctt caactgggca
      781 ttgatttgtg atagattgct cggaggagtc ggcaaagagt taactacgct taatacaaag
      841 ttacattgtc cgattccgct ggcaatggag ctccatgcgc tgttgacatt agggtgctgc
      901 gagctccagg tatgctgcca ttcctggata tgctcgtctc catccagcca gtccgcccca
      961 cggataggaa tgatcatctc gtctgtgctt gcctcaacca gctcacccca gccgctcccg
     1021 ggaataatgc ctgtaagtgt gttgtaggcc gccgcgacgc cggcattgat ttgcgcgggc
     1081 gtctgccaaa aattgctatt ggggaccaca ctgtacaccg tctgattcaa cttcgtacag
     1141 gagtccaccg cacccgccag gaggaaggca gcggttaggc caagataata tgacagtttt
     1201 tttgtcgaca tggcaatctg atttaatatt tcttcagtta tttatttaag gttacgttta
     1261 cgccaaaaga gaacgaacgg gctcttgggt aatagccgtc agcgccgtag ttcgcgtcga
     1321 tataagcttc ctggttgatg ctctggttaa aggggttatt gctgttgccc ggattttgat
     1381 aggaggccgt ccttacctcg gggtccaacc cggaatactt cgtgatcacg aacaaattaa
     1441 ccgccgtgac atatactttg aagttttgga aaccagggac atgaggaata ttataagaca
     1501 aagtaatgtt gtccatgcgc agataccctg cattctccag ccacttcgtt gaagcgaccg
     1561 gctgatccag aattccgttg gacatagccg acgtgaagac gttgttacca ggcaggcgct
     1621 tcaggaaagc gacgtcaagc gcggtgttgt tgaagatctt ttgcccctgc acgccgcgca
     1681 taaagaagtt cagcccccag ttcttataag tgaatgtatt gttgatcccg tagaagaaat
     1741 gagcggtcgg gttgtagtaa tacaaagtgg gattcccgtt tacgccgacc gacttaatgc
     1801 ctgccgagtc aaaaagctgg tttccgttct tgtcgacccc ttcatatttc ggcaattgga
     1861 acacgtaagg agcgtagccc accttcaggt aggttatggg attggagctc agaccccggc
     1921 cttccgcata gccgccggcg atgtagtccg tagacagttt gatgccgcct gcgtaagtcc
     1981 ccgacaggtc ggttacccta gtattgaccg tgctgatatt gccgccgact gtccagctga
     2041 agtctttctc cctaacggca tcgaagctga attgaaattc cacgccttta ttggtcaggc
     2101 ttccgacgtt ggccaggatc gtattaataa agaagggagg cgtgggaacg gtgtaatcat
     2161 acagcaggtc cgtggtcttg tcattatacc agttgaatga accggtcaac cgattgttga
     2221 ataaagtgaa atcaaggccg atgttcgtac cagccctctg ttcccatttc agatccgggt
     2281 tggcgttctg ggtgatgcta tatacgggcg gataagggaa tacggcttta ttagcagcgt
     2341 caaaataggc ggtaccggta gagccgatca ggaacagatt gctatagggg taaatattat
     2401 cagagtttcc ggtcactccg taaccgcccc tgatcttcaa ttcattgatc cagccgatat
     2461 ttttcatgaa atcttcctga ctaatcctcc agcccaggtc aaaggaaggg aacgtaccgt
     2521 accggttgtt cgcgccgaat ttcgatgagc cgtcgcggcg aagggacgct gtaagcagat
     2581 acttgttctg atagctatag gtgaccctgc caagatagga aatcagcagg tagcggtcag
     2641 cgtaggagct tacaacattg taggagggat tgccggcgcc caggttgtta taggtgaggc
     2701 caggaacgat gaattgctgc gctccggcat agaaaccacc ctcgctgaaa ttgttgtact
     2761 cgtaaacggc ggtaaccgtc aggctgtggt ctccccaggt tccgagataa ttggcatgaa
     2821 tgtcgccttt gcccgagtta ttgtagatac tgttctggtt cgcagaagga acgttgcctt
     2881 ctgaaaagcc gtctgttgca aaggtgggct ggaagaactg tttggccagc gaatagtaag
     2941 aaagtgaccc ggtggtgccg agtttcaggc ctttgatcac ctcgaagtta accgtaccat
     3001 acatttgggt aaaatgttcc tgccccttat tgatctcggc gttcaggtgg taaaccgggt
     3061 ttgcttcttc gaaatccacg aacgtatgat aggtgccgtc cgcattgtag acagggtacg
     3121 tcggcggtgc gctaaagatg taatcgaaca cgccgtaatc ggtatatttc ctttggacct
     3181 gtgaattgag gataccgacc tggatgtcca gtctgtcgtt aatcgccttc tcttctgcgt
     3241 tgaaccgtaa cccgatctcc tgtctgcctg aattgagtac gatcccgtta ttgttggtgt
     3301 aatcgacaga agcgcggtag ttaaagttgc cggtgccgcc ggacattccg acattctgac
     3361 tcgtggtagc ggcggtttga gtgatcgcct tgttccagtt cgtactggca ccctggttat
     3421 actgctggat ggtcgaatcc gcgatgccga cctgctttga cgccgcctcc cactggctga
     3481 cgtttgccag aggataatat tttgcaggtt tatcaaaccc tactgcgccg ttatagtcaa
     3541 tctgagacct tccagcgtga cctttcttgg taataatctg aatgacgcca tttgcgcccc
     3601 tggaaccata aatggcggct gccgaagcat cctttaatat ggaataagac tcgatgtcac
     3661 cgggaggaat agtgttgatg atcgtagggt catccagggt cacgccgtcg acaacgatga
     3721 gcggggtttg ggcccccgac agtgaggttt gtcccctgag gtggattatg gggttttgat
     3781 tcgggtcgcc gccttgctgc gtaatgacca gg
//
LOCUS       DATGKE010000395         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_328587, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000395 DATGKE010000000
VERSION     DATGKE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328587"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VKU83_06855"
     CDS             <1..587
                     /locus_tag="VKU83_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93308.1"
                     /translation="WLDAIGGDSNDTNYVFLRFVTDYLPIGLIGLLIAVIMLAAWGSI
                     AAALNALASCTIVDFHRRFSRRVIPPEREYRLSRWYTLLWGIFCVLMAQLAYNVGNSL
                     IEAVNVLGSLFYGVILGVFLVGFYCRKVGGHAVFISAAVVEAGVIALDILGKKGIVPL
                     SFLWFNAVGAVGVILLSNGLQAFMRRRRQPVATP"
     gene            complement(773..1609)
                     /locus_tag="VKU83_06860"
     CDS             complement(773..1609)
                     /locus_tag="VKU83_06860"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HLI93309.1"
                     /translation="MQKLLFLLAFGCAFFVSAPAQTLRTPPISTPQFIRQDFGLSTIE
                     LSYSRPGVKGRTIFGDLVPYGKVWRTGANQATTLTFGDSVTIGGATIPPGKYGLLSIP
                     GADAWTFIITRQTDVTSPAAYREDQDLVRIKAEPHQLPFTVETMTMDFSDLTGSSCRL
                     DLLWDRVAVGFDIRTNTDARVMSQIGAIMNHDTHPYYQAAVYYVENGKDLNKALEWLD
                     KAIAEDPSAYFAVYQKARCLAKLGRKQEAVAAAQSSIELSRKANNPDYVALNEKLIAS
                     LQ"
     gene            complement(1660..>2112)
                     /locus_tag="VKU83_06865"
     CDS             complement(1660..>2112)
                     /locus_tag="VKU83_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93310.1"
                     /translation="GYDLRYLPLTTLSNDVLSDNVIRLNPQRPDLDEVVVTGFGARRK
                     EATMEAPSDDDRSADSAWRFATPAIGRLAYLQYLDSAKKKLALDSTIKGTETISFLVN
                     RNGSLTAFKIEHSLSPAHDAGILRVVSSGPAWILLKGKRARASVTLTF"
BASE COUNT          412 a          514 c          694 g          492 t
ORIGIN      
        1 gatggcttga cgcgatcggg ggcgactcca acgatactaa ttatgttttt ttgcgattcg
       61 tgaccgatta cctccccatc gggttgatcg gcttgctgat tgccgtgatc atgctggcgg
      121 cctggggatc gatcgcggcg gcgttgaatg cgctggcgtc atgcacgatc gtggattttc
      181 accggcggtt ttcgcggcgg gttataccgc ccgagcggga gtaccggttg tcgcggtggt
      241 acacgctgct gtggggcatt ttttgcgtat taatggcgca gctggcctat aatgtcggca
      301 acagtttgat cgaggcggtg aatgtgctgg ggtcgctttt ttacggggtg atactgggtg
      361 tgttcctagt ggggttttat tgccggaaag ttggcggtca cgcggtattc atcagcgcgg
      421 cggtcgtgga ggcaggcgtg attgcactgg acatccttgg gaagaaggga attgtgccgc
      481 tcagcttttt gtggttcaat gccgtcgggg cagtgggggt tattctgctg agcaacggct
      541 tgcaggcgtt catgcgccgg cggcggcaac cggttgccac cccctaaatg aaaaagccgc
      601 tgctccggaa ccggagagcg gcgctatagc tgtgagctga tgtatggtga caggaacttc
      661 tctgttccga agacctcatg ggcgagcgga acgctgcatt aaagtttttt gccgccggcc
      721 ggcttttgcg acaggccatt ttatagcgtg acctaccttt ttatagcgtg acctattgga
      781 gcgaggcaat gagtttctcg ttcagcgcaa cataatcggg gttattcgct ttcctggaaa
      841 gttcaataga ggattgggcc gcggcgaccg cctcctgctt gcggcccagt ttcgcgagac
      901 accgggcttt ctgataaaca gcgaaatagg ctgacggatc ttcggcaatg gccttatcca
      961 accactccag ggccttattg agatctttcc cattctccac gtagtagacc gccgcctgat
     1021 agtaggggtg ggtgtcgtgg ttcatgatgg cgccgatctg ggacatcacc cgcgcgtcgg
     1081 tattggttcg aatgtcgaag ccgacggcga ctctatccca aagcaggtca agtcggcagg
     1141 agctgccggt caggtccgaa aagtccattg tcatcgtctc cacggtaaag ggcagctggt
     1201 ggggttcggc cttgatccgg accaggtcct ggtcctcccg gtaggccgcg gggctggtga
     1261 catccgtctg gcgtgtgata ataaaggtcc aggcatcggc tcccgggatg gacaggagac
     1321 cgtacttccc gggcgggatc gttgcgccgc cgatggtaac ggaatcgccg aaggtgaggg
     1381 tagtggcttg gtttgcaccg gtccgccaga ccttgccgta tggcacgagg tccccaaaga
     1441 ttgtccggcc tttgactccg gggcgtgaat aagagagttc gatggtcgag agaccgaagt
     1501 cctgacggat aaattgggga gtggagatgg gaggtgtcct aagtgtctgg gccggggccg
     1561 acacgaagaa agcgcagcca aacgcaagga ggaatagaag cttttgcatg ttaaggtgtt
     1621 tttgtgctcc gaaattacgg gatgcggagc aatcggaaat cagaacgtga gcgtaacgga
     1681 ggcacgggcg cgcttccctt tcagcagtat ccaggctggc ccgctggaaa cgacgcggag
     1741 aatgccggcg tcatgagccg gagaaaggga atgctcgatc ttgaaggcgg taaggctccc
     1801 gttacggttg accaggaagg agatcgtctc tgttccttta atagtggagt caagcgccag
     1861 tttctttttt gccgagtcga ggtattgcag ataggccagg cggccgatgg ctggcgtggc
     1921 aaatctccag gccgagtcgg cagaccgatc atcgtccgag ggtgcttcca tagtggcctc
     1981 tttgcgacga gcgccaaagc ctgtgacgac gacttcgtcg aggtcgggac gctgggggtt
     2041 cagccgaatc acgttgtcgc taagtacatc gttgctcagc gttgtgagag gaagatatcg
     2101 caaatcatag cc
//
LOCUS       DATGKE010000396         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_218968, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000396 DATGKE010000000
VERSION     DATGKE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218968"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VKU83_06870"
     CDS             complement(<1..313)
                     /locus_tag="VKU83_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HLI93311.1"
                     /translation="MIHSILKDRPTKLLLGFTAFFCCNALIADAIGTKLFSLEKVFGV
                     RPANWTLFGQGGLGFTLTCGVLLWPLEFVITDIINEFYGPRVVRRISYTAVALISYAF
                     LM"
     gene            657..1988
                     /locus_tag="VKU83_06875"
     CDS             657..1988
                     /locus_tag="VKU83_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93312.1"
                     /translation="MEPIKILLLNYYWPPSGGPAVQRWLDITRYLAAYNIHTYVVTVD
                     EKKATYPFIDETLLSRIDSSTQTFRTGTSELFGIYKRFIGKGKVPASGLVDEPEPTFL
                     QKVARFARGNFFLPDPRKGWNKHAYARAAALLRMEKIDIVFTAGPPHSTHLVGLRLKK
                     HFPHLRWIADIHDYWTEISYLSKFYRTAIAGYFDQRLEKEVLLAADEIMTHCQSSKSL
                     QCKKLGGRDCSKIFVHTMGFSEDLFNPRPPSRQDRFEISYTGIMADHYEPEMLFRALK
                     TAMAERPDVPVRLKFVGILAPTIRIRIIELGLGPILEELPYMSHAGVIGELYKSTAVF
                     LINPKFENEGIHVPGKIYEYLAVFKPIISISPHGSENEQIILKMKAGRNFDRSEEGQL
                     SRYLIELMDRWEQDKNVDLPENPDIRIYGRRHESARLRDRIEGLVNKKTPA"
     gene            complement(2019..2444)
                     /locus_tag="VKU83_06880"
     CDS             complement(2019..2444)
                     /locus_tag="VKU83_06880"
                     /inference="COORDINATES: protein motif:HMM:NF023109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HLI93313.1"
                     /translation="MAYDNNDKRMESVYSKRIRAGKRRTYFFDVRATRSNDYYLTITE
                     SRKKFDQDGYDRHKIFLYKEDFNKFLKALTEAVDYVKTDLMPDFDFDAYNHDNDGENQ
                     PTFTENGVTEEIEIEVREVREEPAAPSAPTATGEEVDKW"
     gene            complement(2682..>3170)
                     /locus_tag="VKU83_06885"
     CDS             complement(2682..>3170)
                     /locus_tag="VKU83_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI93314.1"
                     /translation="QDVFLFSDTVSGNIQFGLEKPDEEKVRVAAHYASVDKEIEGFSD
                     RYNTMIGERGVTLSGGQKQRVSIARGLIKDPEIIVFDDCLSAVDAKTEKEILGNLYQY
                     LQHKTAIIITHRIFSLFDFDRIVVLDEGRIVETGTHRKLIEINGYYTYLYEQQQAENN
                     TA"
BASE COUNT          803 a          868 c          759 g          740 t
ORIGIN      
        1 acatcaggaa ggcgtaggag atgagcgcga ctgccgtgta ggagatgcgc cgtacaacac
       61 gcgggccgta gaattcattg atgatatcgg tgatcacaaa ctcgagcggc caaagcagta
      121 ccccgcaagt cagggtaaat cccaatccgc cctgcccgaa gagcgtccaa tttgccggac
      181 gaaccccaaa caccttctcc agcgaaaaca gcttcgtccc gatcgcatcc gcgatcagtg
      241 cattgcagca aaaaaatgcg gtaaatccca gcaagagctt tgtcggtctg tcttttaaga
      301 tggaatggat catctcagct gatgtattaa cgaaattgac tggaggcaga aaatggtaaa
      361 gttaaaggcg ggaagccaaa cgggcaaggc agcggaccgc catcgccccg cgaaacgcag
      421 gctgagcgac cagcttaaga caaccgcatt gacaggaagg cccatgaggc aacccataac
      481 gattatcgtc gaaaccagtg acccccgcgg cgggctgggc agccgcagaa ggagcaaggc
      541 agccaggaaa caaatattgc atataaacgc tatccgcatg agaaccttaa acagctgcat
      601 atttggccaa attctgtatt tctgggcaaa ataccattat tttgtcagcc gctgccatgg
      661 agccgatcaa aatactattg ctgaattatt actggccgcc cagcggcggt cccgccgtgc
      721 agcgctggct tgatatcacc cgctaccttg ccgcctacaa cattcacacc tatgtggtca
      781 ccgttgatga aaagaaggca acctatccct tcatcgatga aacgctgctg agccggatcg
      841 acagctcaac ccaaaccttc aggaccggga cttccgaact gttcggcata tacaagcggt
      901 ttatagggaa aggcaaagtg cctgccagcg gtctcgtcga cgaacccgaa cctaccttcc
      961 tgcaaaaggt cgcccggttc gcacgaggaa atttcttcct ccccgacccg aggaaaggct
     1021 ggaacaaaca tgcttatgcc cgcgcagccg cattactccg tatggaaaag atcgatattg
     1081 tcttcaccgc gggacccccg cattcgaccc acctcgtcgg cctcaggcta aaaaagcatt
     1141 ttccgcatct ccggtggatt gccgacatcc acgactattg gacagagatc tcttatttat
     1201 caaaattcta ccggacggct atcgcaggct attttgacca gcgcctggag aaggaagtat
     1261 tgctggcggc agatgagatc atgacccatt gccagtcgtc aaaatcccta caatgtaaaa
     1321 agttaggggg ccgtgattgt tcgaagatat ttgtccatac catgggcttt agcgaggacc
     1381 tgttcaaccc ccgcccgcct tcaaggcagg atcgctttga aatcagctac acgggcatca
     1441 tggccgatca ttacgaaccc gaaatgctgt tcagggcctt gaaaaccgct atggccgaac
     1501 gcccggacgt tccagtcagg ttaaaatttg tagggattct ggcgcccacc atccggatcc
     1561 gcattattga gcttggatta ggccccattc tggaggaact gccttatatg tcgcacgccg
     1621 gtgtgatcgg ggagctctat aagagcaccg cggtattcct gatcaatccg aaatttgaga
     1681 acgaaggaat tcatgtaccc ggcaagatct acgaatacct tgcggtgttc aagccaatta
     1741 tcagcatctc tccccacggc agtgaaaatg agcagatcat ccttaagatg aaagccggcc
     1801 ggaactttga ccgttctgag gaagggcagc tcagccgtta cctgatcgaa ctgatggatc
     1861 ggtgggaaca ggacaaaaac gttgacctcc cggaaaaccc ggatatccgg atatatggaa
     1921 gaaggcatga gtcggcaaga ctccgggaca ggatcgaagg gctggtaaat aaaaaaaccc
     1981 ctgcatagca ggggtccggt atagtgcctc aagtataatt accatttatc aacttcttcc
     2041 ccggtcgccg tgggagcgct gggagcagcc ggctcctcac gaacttctcg cacctctatc
     2101 tctatctcct ccgttacgcc attctccgta aaagtaggct gattctcgcc atcgttgtca
     2161 tggttgtagg cgtcaaaatc gaagtccggc atcagatccg tcttgacata atccaccgct
     2221 tccgtcaggg ccttcaggaa cttattaaaa tcttccttat ataagaagat cttatgcctg
     2281 tcgtaaccat cctggtcgaa tttcttccgg ctctccgtaa tagtaagata gtaatcatta
     2341 ctccgggtag ctctcacgtc aaagaaatac gttctccgtt tccccgccct gatgcgcttg
     2401 ctataaacgc tttccattct cttgtcgttg ttgtcgtacg ccacaatgat ttgttttgca
     2461 gttgaaaatt acaaattaac tagccacaaa tatagtattg ttttcgaatt accaaaattt
     2521 gccagaagaa ttgtatctgc taaccaaggc tttttcgacg agtgtttgcg gcctgctccg
     2581 gcgaagcttt ccgcttgcct gtccgcggtt cgacgactgc tccccagcag gttcccgcct
     2641 ggcggctcct gatctgacca gatcaagcca ggcccgccag ctcaagccgt attattctca
     2701 gcctgttgtt gttcatacag ataggtgtaa tagccgttga tctcgatcaa tttccggtgg
     2761 gtgcccgtct cgactatcct tccttcatcg agaacgacga tgcggtcgaa gtcgaatagg
     2821 gaaaaaatgc gatgagtgat gattatcgcc gtcttatgct gcaggtattg atagagattg
     2881 cccaggatct ccttttccgt cttggcatcc actgcactca gacagtcgtc aaatacaatg
     2941 atttccgggt ccttgatcag gcctctggca atagataccc gctgcttttg gcctccgctg
     3001 agcgtaacgc cccgctcccc gatcatcgta ttgtaacggt cgctaaaccc ttcaatttcc
     3061 ttgtcgacgc tggcataatg ggccgctacc ctgaccttct cctcgtccgg cttctcgagg
     3121 ccgaactgga tattacccga gacggtatca ctaaagagga aaacatcctg
//
LOCUS       DATGKE010000397         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1260835, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000397 DATGKE010000000
VERSION     DATGKE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260835"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VKU83_06890"
     CDS             <1..943
                     /locus_tag="VKU83_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93315.1"
                     /translation="KGWLRGCDFIPSSAVNQLEMWQAGTFDPATIDRELGYAQSVGLN
                     CMRVFLHHVAWEEDPSGFKARVNRYLTIADRHGIKTIFVFFDDCWNAVYHAGLQPAPR
                     PGIHNSGWLRDPGQLYYDEPRLIDTLHTYVADILTAFKHDKRILLWDLYNEPGNSGYG
                     NKSLPLLEKAFIWGREINPDQPLSAGVWSDRLTTLNAFQLSHSDVITYHDYETPDKHQ
                     RCIDTLRKYDRPLLCTEYMARLRGSTFFNVMPLLKKEKVAAINWGLVTGKTNTKYAWD
                     TPMPDGAEPKVWFHDIFRANGTPYDPKETAFIRSLTQ"
     gene            1039..2277
                     /locus_tag="VKU83_06895"
     CDS             1039..2277
                     /locus_tag="VKU83_06895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLI93316.1"
                     /translation="MKRKTDNFNKFYNKKRGSAIKEEIRQEKKAAKKERRDAIERHFE
                     DRRQGRDLNAKGGAAPVGGRAGKAKFEPRRAVKGKIEPAGAARGKAAPAEAGAARGKA
                     GPERGRFVPAGPTGAAKGKVVPAGDRAEKGKIALVKEKIGEQLPLNKYIAHSGLCSRR
                     DAAELVRQGKVTVNGKPVTEPGTKVVPTDLVKVNGKKLTMSRNFVYILLNKPKDYITT
                     MEDPQGRRTVLDLIRAATTERVYPVGRLDRNTSGVLLLTNDGDLAQKLAHPSHEIKKV
                     YHVTLDKPLTKADFDKIIGDGIVLEDGPANVDVLAYADPRDKTQIGLEIHSGRNRIVR
                     RIFEHLGYDVRGLDRVMYAGLTKKNVQRGHWRLLSEKEVRILKYLNSAIKGSTARAKD
                     WDTLPAAAKHARSKFHPEGD"
     gene            2287..>2943
                     /locus_tag="VKU83_06900"
     CDS             2287..>2943
                     /locus_tag="VKU83_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HLI93317.1"
                     /translation="MHHPTILFENEAFIVIDKPAGFLSIPDREGKEPSLKSWLREKYG
                     NIYVVHRLDRETSGVIVFAKDEAMHKYLSGIFENREVEKQYQGIVVGTLPNQQGSVDL
                     PLMEHPTRAGVMVTNRRGKASLTDYQVLEELGAYSLVQFRIHTGRTHQIRVHMQALGN
                     PVACDELYGDGRPVYLSGIKRNYKLSKDQEQERPIVARLALHSQLLRFADAAGVLHTF
                     E"
BASE COUNT          705 a          759 c          894 g          585 t
ORIGIN      
        1 gaagggttgg cttcgcggct gtgatttcat accgtcgtcc gctgtgaacc agctcgagat
       61 gtggcaagcc gggacgttcg accccgcgac gatcgacaga gagctcggct atgcgcagtc
      121 ggtcgggttg aattgcatgc gcgtcttcct tcatcatgta gcgtgggagg aggacccttc
      181 gggtttcaag gcgcgggtga accggtatct taccatcgcg gatcgacatg gcataaagac
      241 catcttcgtg tttttcgatg actgctggaa tgccgtttat catgcagggc tgcagcccgc
      301 gcccaggccc ggtatccaca attccgggtg gcttcgtgat ccgggtcagc tttactatga
      361 cgagccgcgg ctcatcgaca cgctgcatac ctatgttgcc gacatcctca ccgccttcaa
      421 acacgacaaa cggatcctgc tctgggacct ttataatgaa ccgggtaatt ccggttatgg
      481 gaacaagagc ctgccgttgc tcgaaaaggc gttcatctgg gggcgtgaaa tcaatcccga
      541 tcagccgctg tctgcggggg tatggagcga ccggctgacg acacttaatg cttttcagct
      601 tagccattcg gatgtgatca cgtatcacga ttatgaaacg ccggacaagc atcagcggtg
      661 catcgatacg ttgcggaaat acgatagacc gctcctctgc acggaatata tggcgcggct
      721 gcgcggcagc acgttcttca atgtcatgcc gctgttgaaa aaggagaagg tagcggcgat
      781 caactggggg ttagtcaccg ggaagaccaa taccaaatat gcgtgggata cgcccatgcc
      841 ggatggggcg gagccgaagg tatggtttca tgatatattt cgggcgaacg ggacgccgta
      901 tgatcctaag gagacggcgt ttataaggtc gctcacgcag taggcgcggc ggccaggcgt
      961 cctttgcggg cgggcccgca gcgggaacgc cggatatttt tcgtactttt gtcggctact
     1021 gttgtaaaac agcaatttat gaagcgaaaa acggacaatt tcaacaagtt ctataacaag
     1081 aaaagaggga gcgccatcaa ggaggagatc cgccaggaga agaaggcggc caagaaagag
     1141 cgccgcgacg ccattgaacg gcattttgag gataggcggc aggggcggga tctcaacgcg
     1201 aaaggcgggg ctgctcctgt ggggggccgg gccggtaaag cgaaatttga gccgaggagg
     1261 gccgtgaagg ggaagatcga gccggcgggg gctgccagag ggaaagctgc gccggcggag
     1321 gctggggctg ccagagggaa agctggtccg gaaagaggga ggtttgtgcc cgcggggccg
     1381 accggggctg ccaaagggaa agtggtcccg gcgggggata gggccgagaa gggaaagatt
     1441 gcgttggtga aagaaaaaat tggcgaacaa cttccattaa ataaatatat cgctcatagc
     1501 ggcttgtgtt cgcgccggga tgctgccgag ctcgtccggc aagggaaagt cacggtcaac
     1561 ggcaagcctg tcaccgaacc cggaacgaag gtagtgccca ccgatctcgt caaagtcaac
     1621 ggcaaaaagc tgacgatgtc gagaaacttc gtctacattc tgctcaacaa accaaaggac
     1681 tacatcacca ctatggaaga tccgcagggg cgccgtaccg tgctcgatct tattcgtgcg
     1741 gcaacgacgg aacgggtgta ccccgtaggg cggctggacc gaaatacatc gggtgtgctt
     1801 ttgctcacca acgacggcga tcttgcccag aaactcgcac atccttccca cgaaataaaa
     1861 aaggtctacc atgtgaccct ggacaaaccg ctcaccaaag ccgatttcga taagatcatc
     1921 ggcgacggta tcgtcctcga agatggacct gccaatgtcg acgtcctggc ttatgccgat
     1981 ccgcgggaca agacgcagat cgggctcgag atccacagcg gacgcaaccg tattgtccgg
     2041 cgcatctttg agcatttggg ctatgatgtc cggggcctgg accgcgtcat gtatgcggga
     2101 ctcaccaaga agaatgtaca acggggtcat tggaggcttc tttcggagaa agaggtaagg
     2161 atactcaaat acctgaactc tgccatcaag ggatccacgg cgcgggctaa agattgggac
     2221 actcttcccg ctgcggccaa gcatgcgaga tccaaattcc atcccgaagg cgattaaacc
     2281 gagctcatgc atcatccaac gattctcttc gaaaacgaag cctttattgt catcgataaa
     2341 cctgcggggt tcctgagcat tcccgatcgc gaaggaaagg aaccctcact taaatcgtgg
     2401 ctaagggaaa aatacggtaa catctatgtg gtgcaccggc ttgaccgcga gacgagcggc
     2461 gtcattgtct ttgcgaaaga tgaggccatg cacaagtacc tctcgggtat cttcgagaat
     2521 cgggaggtgg agaaacagta tcagggtatt gttgtgggca cacttccgaa ccagcagggc
     2581 agtgtcgatc tcccgctcat ggagcatccg acacgagcgg gcgtaatggt caccaatcgc
     2641 cgcggcaagg cctcgctcac cgactatcag gtcctcgaag aactcggcgc gtactcgttg
     2701 gtacaattcc gcattcatac cggcaggacg catcagatcc gggtgcacat gcaggcgttg
     2761 ggcaacccgg ttgcctgcga cgagctctat ggagacgggc gacctgtgta cctctccggc
     2821 atcaagcgga attataaact atccaaggat caggagcagg aaaggcctat cgtggcgcgg
     2881 cttgcgctgc attcgcaatt gttgcgtttt gccgatgcgg ccggggtgct gcataccttt
     2941 gag
//
LOCUS       DATGKE010000398         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1125636, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000398 DATGKE010000000
VERSION     DATGKE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125636"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VKU83_06905"
     CDS             complement(<1..393)
                     /locus_tag="VKU83_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93318.1"
                     /translation="MLQAPDILQMAYRQPVLQEDLVVLHEKDQQIPGSVQYAIRRYRK
                     NPHWNLDDVGMLVYQNSAPKKGAETQRYLELRFCITGNTYCRQKAVECDSCKLKASPG
                     CTERVDTIDVFHFRFQAVHLSQFIRPRRP"
     gene            551..>2389
                     /locus_tag="VKU83_06910"
     CDS             551..>2389
                     /locus_tag="VKU83_06910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HLI93319.1"
                     /translation="MSESTQEVAVPATNGYGADSIQVLEGLEAVRKRPAMYIGDISKK
                     GLHHLVYEVVDNSIDEALAGYCKNILVSIHEDNSVEVVDDGRGIPTGINSKEKKSALE
                     LVMTVLHAGGKFDKNTYKVSGGLHGVGVSCVNALSSDLHVTVFRDGKIFEQGYKIGVP
                     QYAVREIGESDKTGTTVRFWPDASIFTETVFDKDILEGRLRELAFLNRGISITLNDLR
                     EKDDEGRTYSKTFFSQGGIVEFVEMLDKNASRLPLIPKIIYVEGRDEATNVAVEVALT
                     YNDGFSEHIYSYVNNINTIEGGTHVAGFRRALTRVFKSYGDREGLFERAKVDIEGDDF
                     REGLSTIISVKVPEPQFEGQTKTKLGNTEVSGVVDSTVSRALDSYLEENPREAKNIIN
                     KVILAAQARAAAKKARELVQRKSVLSGGGLPGKLADCSDRDPNRCELFLVEGDSAGGT
                     AKQGRDRSFQAILPLRGKILNVEKAMEHKIYENEEIRNMYTALGVTVGTPEDPKGLNL
                     LKLRYHKLIIMTDADVDGSHIATLILTFIYRYMKEMVEQGYVYIAQPPLYLVKKGKEQ
                     SYAWNEDQRRGLVERLGGGKEDSVVVQRYKGLGEMNSDQLWDTTMNP"
BASE COUNT          597 a          614 c          708 g          470 t
ORIGIN      
        1 cggccgccgc ggccggatga attgcgaaag gtgtaccgcc tggaagcgga aatggaaaac
       61 atcgatcgta tcaacgcgtt ccgtacaccc gggactcgcc ttgagcttgc acgagtcgca
      121 ttcgacagct ttttggcggc aatacgtgtt tccggtgatg cagaaccgca gctcgaggta
      181 tcgctgcgtc tcggcgcctt ttttcggcgc agaattctgg tagaccagca tcccgacgtc
      241 gtcgaggttc caatgggggt tcttccggta ccgccggatg gcgtactgga cggagcctgg
      301 gatttgctga tccttttcgt gaaggacgac gaggtcttcc tgcaaaacgg gttgccggta
      361 ggccatctgc aaaatatcgg gtgcctggag cattttgcaa atttacatcg aaaggcgtga
      421 atacatgcta tgatccggga agaaaaaata ggcccgtttg aaggctgaaa aacagcgaaa
      481 aggccttatt tttcgtacat ttgcaagcct ttattaattt attgttaact acactattcc
      541 agtcaaaaaa atgagtgaat caacacagga agttgcggtt ccggcgacca atggttacgg
      601 ggcggatagc atccaggttc tggagggttt ggaagcggtc cgcaagcgcc cggccatgta
      661 tatcggcgac atcagcaaga agggtctcca ccacctggtc tacgaggtag tggacaactc
      721 gatcgacgag gcgctggccg gctactgcaa gaatatcctc gtgtctattc atgaagataa
      781 ttcggtcgaa gtggtcgacg acggccgggg cattccgaca ggtatcaaca gcaaggaaaa
      841 aaagagcgcc ctggaactgg tcatgaccgt ccttcatgcc ggcggcaaat ttgacaaaaa
      901 tacgtacaag gtatcaggcg gcctccatgg cgtgggggta agctgcgtca atgcgctcag
      961 ctcggacctg cacgtgaccg tctttcgcga cggcaagatc tttgagcagg gatacaagat
     1021 cggggttccg caatacgccg tccgggagat tggcgaaagc gacaagaccg gcaccacggt
     1081 tcgtttctgg cccgacgcct ccatctttac ggaaacggtt tttgacaagg atatcctcga
     1141 aggaaggttg cgcgagctcg ccttcctgaa ccggggcatc agcatcaccc tcaacgatct
     1201 tcgcgaaaag gacgatgagg gccgcaccta cagcaagact tttttcagcc agggcggcat
     1261 tgtcgagttt gtggaaatgc tggacaagaa tgcaagccgc ctccccctga tcccgaagat
     1321 catttatgtg gaaggacgcg acgaagcgac gaatgtggcc gtcgaggtgg cgctcacgta
     1381 caatgacgga ttcagcgaac acatttattc atacgtcaac aacatcaata ctattgaggg
     1441 cggcacccat gtcgccggct tccggcgcgc cctgacgagg gttttcaaat cgtacgggga
     1501 ccgggaaggg ctattcgaaa gggcaaaggt ggacatcgag ggcgatgatt tccgggaagg
     1561 actgagcacg atcatttcag tgaaggtccc cgagccgcag ttcgaagggc agaccaaaac
     1621 caaattgggc aataccgaag taagcggggt cgtcgattcg acagtaagca gggcgctcga
     1681 ttcctacctc gaggaaaacc cgcgcgaggc aaagaacatc atcaataagg tcatcctcgc
     1741 cgcccaggcc agggcggcgg ccaaaaaagc ccgcgagctc gtacagcgca agtcggtact
     1801 gagtggcggt ggtctgccgg ggaagcttgc cgactgttcg gaccgcgacc cgaaccgctg
     1861 cgagcttttc ctcgtcgaag gtgactccgc cgggggcacc gccaaacagg gccgggaccg
     1921 cagcttccag gcaatcctgc cgctccgggg aaagatcctc aatgtggaga aggcgatgga
     1981 gcataagatt tacgagaacg aagagatccg caacatgtac acggcccttg gggtaaccgt
     2041 aggcacgcca gaggatccca agggcttgaa cctgttgaaa ctgcgctacc ataagctgat
     2101 catcatgacg gatgcggatg tggatggcag ccatatcgcc acgctgatcc tgacctttat
     2161 ctaccggtat atgaaggaaa tggtcgagca gggttatgtg tatatcgccc aaccaccctt
     2221 atacctcgtg aaaaaaggta aggaacagtc ctacgcgtgg aacgaggatc agcgccgcgg
     2281 actcgtagaa cgcctaggcg gcggaaagga agacagtgtg gtcgtacagc ggtacaaggg
     2341 acttggtgaa atgaactcgg atcagctgtg ggacacgacg atgaacccg
//
LOCUS       DATGKE010000399         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_165698, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000399 DATGKE010000000
VERSION     DATGKE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165698"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VKU83_06915"
     CDS             complement(<1..762)
                     /locus_tag="VKU83_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLI93320.1"
                     /translation="MPTREPRQHDSHSHSHGGVGGHGHHHSHEDTHDHSHGFGHHHHP
                     VDLEQVNRAFVIGIILNFVFVLIEVIVGLDIHSLSLLSDAGHNLADVASLAMALFSIR
                     LLRVRATEKYTYGFKKTTILVALLNAAILLVSIGAIGYEAVHRLMTPETRLPGKVISI
                     VAAIGIGINGLTAMLFFRNKESDLNVRGAFLHLLSDAIVSAGLVIGGLVIFYTSQYWI
                     DPVLSLAIAIVILYSTWQLLRDSLRLSLDGVPAGIE"
     gene            complement(772..1086)
                     /locus_tag="VKU83_06920"
     CDS             complement(772..1086)
                     /locus_tag="VKU83_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLI93321.1"
                     /translation="MEYLTDIPAREIRPGFFGKLVHGEKSTLAIWEIKKGSRLVEHSH
                     HHEQITFVLEGELEMIIGGEKYLMKAGATHAIPSNVPHSAFAHTDVKVIDSFAPARDD
                     YR"
     gene            complement(1141..1923)
                     /locus_tag="VKU83_06925"
     CDS             complement(1141..1923)
                     /locus_tag="VKU83_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93322.1"
                     /translation="MDLQLTGKTALVLAASKGLGKACATTLAAEGANVVIGARNPETL
                     QATQKEIAALGKGRVLAVPIDVLRPADAGKIVMATIEEFGRLDILVNNAGGPPFGKFE
                     AFDEAAWQSAFELTLLSVTRFCRLAIPHLRKTGSGRIINITSLTVLTYLPYSVLSTSL
                     RMGVVGMAKMLSEELGPDRITVNNVAPGLILTDRVKDTLQPRPGVSEEELLAERARTI
                     PIGRLGRPDELAALVAFLASPLAAYITGATIPVDGGAVRKPL"
     gene            1970..2395
                     /locus_tag="VKU83_06930"
     CDS             1970..2395
                     /locus_tag="VKU83_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93323.1"
                     /translation="MEKVLQILLVAGLLMFVIWRSIRGKDEWFRRWSYQVRLRAPLPE
                     STGERLLTGTFGPGVERVNHAVPDDADAWLSDEDRRILLCYTMDGKMTIYRLGYGGGK
                     REFQEMSVPMDCTALAWDPEERKIYLEEGGYWYVYGPEG"
     gene            complement(2448..>3033)
                     /locus_tag="VKU83_06935"
     CDS             complement(2448..>3033)
                     /locus_tag="VKU83_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93324.1"
                     /translation="ERNQLFYLHPVTNRRTHISRIYNRVIFDELHHAAANLGQHVDIR
                     QDLDVQWAAHPDWFYRVSKFALPFIHHPYAPEAFFLNDLKQPPGDLHNYVLKPLFSFA
                     GQGVIIDPEISDLDVEDPENWILQRKVKYADLIPTPDVPAKAEIRMLYLWPDKASRPT
                     LAINLARLSKGKMIGVNFNRDREWVGGSVAFVER"
BASE COUNT          630 a          762 c          990 g          651 t
ORIGIN      
        1 ttcgatcccc gccggcaccc catcgagcga tagccggagg ctgtcacgaa gcagttgcca
       61 ggtgctatat agaatgacga tggcgatcgc caggctcaac accgggtcga tccagtattg
      121 gctggtgtag aagataacga ggcctccgat gaccagcccc gcggaaacaa tagcatcgga
      181 caataggtgg aggaaggcgc cgcgaacgtt gaggtcgctt tccttattcc ggaagaacag
      241 catggcggtg aggccgttga tgccgatgcc gatggcagcc acgatggaga taacctttcc
      301 cggcagacgg gtttccggcg tcatgaggcg atggaccgct tcgtagccga ttgcgccgat
      361 ggacaccagg aggatggcgg cattgagcag cgccacgagg atcgtggtct tcttgaagcc
      421 gtaggtatat ttttcggtag cgcgaacccg aagcaagcgg atggagaaga gtgccatggc
      481 gaggcttgcc acatcggcaa ggttgtgacc ggcatcggaa agcagcgaaa gagaatggat
      541 atcgaggccg acgatcactt ctatcagaac aaaaacgaaa ttcaggataa tgccaataac
      601 gaacgcccgg ttgacctgtt ccagatcgac gggatggtga tggtggccga agccgtggga
      661 atgatcatgg gtgtcttcgt gggaatgatg gtgcccgtga ccgcccacgc ctccgtggga
      721 atggctgtgg ctgtcgtgct ggcggggttc tcttgtcggc atggttggat tttaacggta
      781 gtcatctctt gccggcgcaa agctgtcgat aaccttcacg tcggtatggg caaatgcgga
      841 gtgagggacg ttcgaaggta tcgcgtgggt cgcgcccgcc ttcatcaggt acttctctcc
      901 gccgataatc atttccagtt cgccttccag cacgaaggta atttgttcat ggtggtgact
      961 gtgttcgacg agccggctgc ccttcttaat ttcccagatg gccagggtgc ttttctcgcc
     1021 atgcaccagt tttccaaaga aaccgggcct tatctccctg gccggaatgt cggtgagata
     1081 ttccattact gattttggct cgaagttacg aaaaaagcgg ccatcgcgcc ctttgccgcg
     1141 tcaaagtggt ttccggacgg cgccgccatc aacgggaatg gttgctccgg taatatatgc
     1201 tgcaagaggg gaggccagga aggcaacgag ggctgcgagt tcatcgggtc ggccgaggcg
     1261 cccaatggga atggttcggg cacgctcggc taacagctct tcttcactta ctcccggtct
     1321 gggctgcagc gtatctttta cgcggtcggt gaggatgagc ccgggcgcca cattgtttac
     1381 cgtgatccgg tcgggcccta actcttccga aagcattttg gccattccga ccacccccat
     1441 ccgcaggctg gtggagagca cggaataggg caggtacgtc agcaccgtca agctggtgat
     1501 attgataatg cggccgctgc cggtcttccg gagatggggg atggcgagcc ggcaaaagcg
     1561 cgtgacactc aggagggtca actcgaatgc cgattgccag gctgcctcgt caaatgcctc
     1621 aaattttcca aagggaggac cgccggcgtt attgacgagg atatccaggc ggccgaattc
     1681 ttcgatggtg gccatgacga ttttgccggc gtccgcgggc cgcaggacgt cgatgggcac
     1741 cgcgagaacg cggcccttgc cgagggcggc tatctctttc tgcgttgcct ggagggtttc
     1801 cggattccgg gctccaatga cgacgttcgc gccttccgcg gcgagcgttg tggcgcaagc
     1861 tttgcccagg cccttgctgg cggctaacac gagcgccgtt ttgccggtaa gttgcaaatc
     1921 catgaatgcg gcttgaggaa ataaagatac gcggtatctt gcgatcgcga tggaaaaggt
     1981 cctacagata ttgctggtcg ctggcctgtt gatgttcgtg atctggcgta gcatccgggg
     2041 aaaggacgaa tggttccggc ggtggagtta tcaggtgcgg cttcgggcgc cgttgccgga
     2101 gagcaccggc gaacggttgc tgacagggac atttgggccg ggcgttgagc gcgtgaacca
     2161 tgcggtacct gacgatgcgg atgcctggct atcggatgaa gaccgccgga tattgttatg
     2221 ttacactatg gatggcaaga tgaccatata ccggctcgga tatgggggcg ggaagcggga
     2281 gttccaggaa atgtcggtgc cgatggactg cactgcgctg gcttgggatc ctgaggagcg
     2341 aaaaatatac ctcgaggaag gggggtactg gtatgtatat ggaccggagg gataaggcag
     2401 gactggacac ggcgggcgcg tgggcggttc cagacattaa gcggacgcta gcgctcgaca
     2461 aaggcgacgc tgccgccgac ccattcccgg tccctgttga aattgacacc gatcattttt
     2521 cccttactca ggcgcgcgag gttgatggcg agggtggggc ggcttgcctt gtcgggccat
     2581 aggtacaaca tgcggatctc ggccttggca ggaacatcgg gggtgggtat aagatcagca
     2641 tatttgacct tccgctggag gatccagttc tcaggatctt caacgtccag gtcggaaatc
     2701 tccggatcga taatcacgcc ctggccggca aaggagaaaa gcggtttcag gacgtagttg
     2761 tgcaggtctc ctgggggctg tttcaggtcg ttgaggaaga atgcctcggg cgcataggga
     2821 tggtgaatga acgggagggc gaatttgctt acgcggtaaa accagtcagg atgcgcggcc
     2881 cattgcacgt cgaggtcctg acggatgtcc acgtgctgtc ccaggttggc agccgcatgg
     2941 tgaagttcat cgaagatgac ccggttataa atgcggctga tgtgcgtccg ccggttggta
     3001 accggatgca gatagaataa ttgatttcgc tcc
//
LOCUS       DATGKE010000400         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_131040, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000400 DATGKE010000000
VERSION     DATGKE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131040"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VKU83_06940"
     CDS             complement(<1..1607)
                     /locus_tag="VKU83_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93325.1"
                     /translation="MFVNYLKVTFRNLLRHKTFSFINVFGLAVAMAVCMLIMLIIGDQ
                     KSYDRFQKNRDRIYRIETTGKDGKGMRTATSALPLGALLRTQYSGIEAAASLVKNIGG
                     DLLYKDKIASGGGYFADDNLFRVMDFQLERGDTATALAKPFTMVISKEIADQLFRGQD
                     PIGKTISFNDTGINPSGPETGNKETPYGRFTITGVLRPNPGKTSLPFKLLASLSSLPE
                     LTRDSILNYPPDNWDNVWSNYTYVLMEKGRSQAYLQQVLDRVSAGQYPKGAPDQFAFH
                     AVALTDIMPADPIGNPTNTGMPKLVLYVLSVLCLIVMLSACLNYTNLSIARQLTRAKE
                     VGIRKTSGATRGQIFTQFISEAVTMSLVSLVVSFGIVLFFQHLFAGLWLNRFLSISFH
                     YTPGLVLLFIGFAIVVGIVAGLLPSLYMSLFNPINILKGLNHFKMFKRLTVRRILLVT
                     QFCVSLVFIISTSLIYLQGDHILNFNYGFDKDNVVNIKMFKAENYPRFLQAISQSRYI
                     DAVSTCAFLPATGTDMATQVRPAANLK"
     gene            1720..1905
                     /locus_tag="VKU83_06945"
     CDS             1720..1905
                     /locus_tag="VKU83_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93326.1"
                     /translation="MESPPVRPLMAAGSKARWIYRKTRDGKPWHLRGVDQAAAARAKQ
                     PRPAARSRDRRQTPALR"
     gene            1939..2352
                     /locus_tag="VKU83_06950"
     CDS             1939..2352
                     /locus_tag="VKU83_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93327.1"
                     /translation="MNWRLIFVLSLFGLAMAFATITVIPTYREPWFFLVIFLICAYCI
                     AKWAPGKFFLHGFLVSLLNCVWITAVHIMYSTTYLAHHAQEASQYSKMNAQLGVSPTV
                     AMLLVGPVIGVVSGLILGLFAFVASRMVKSHLAPL"
BASE COUNT          532 a          640 c          690 g          532 t
ORIGIN      
        1 tccttgaggt ttgccgccgg acggacctgg gtcgccatgt ccgtgccggt cgccggcagg
       61 aaggcgcagg tcgaaaccgc gtcaatgtac cggctttgag agatcgcctg caggaaccgg
      121 ggatagttct ccgctttgaa catttttatg ttgacgacat tgtccttatc aaatccataa
      181 ttgaagttca ggatgtgatc gccctggaga tagatcaggc tggtcgaaat gataaagacc
      241 agggatacac aaaattgggt gaccagcaat atgcggcgca ccgtcagcct tttgaacatt
      301 ttaaaatggt tcaaaccctt cagaatatta atcgggttga aaagcgacat atacagcgaa
      361 ggcaggagcc cggccacgat tcccaccaca atggcaaacc cgataaaaag aaggaccagg
      421 cccggggtat aatgaaaact aatactcagg aaccggttca gccacagccc ggcgaacagg
      481 tgctgaaaga acagcacgat cccgaacgac acgacgaggg ataccaggga catggtgacc
      541 gcctcggaaa tgaactgcgt gaagatctgg ccgcgggtcg cgccggacgt tttgcgaatg
      601 cctacttctt tggctctggt cagctgcctg gcgatggaaa ggttcgtata gttcaaacag
      661 gcgctcaaca tcacgatcag gcaaagaaca ctcaaaacat acaggaccag cttgggcatg
      721 cccgtattgg tgggattgcc gatagggtcc gccggcatga tatctgtcag cgccacggcg
      781 tgaaaagcga attgatccgg cgcgcctttc gggtattgcc cggccgagac ccgatcgaga
      841 acctgctgga ggtacgcctg tgatctgccc ttctccatca ggacataggt gtagttgctc
      901 cagacattgt cccagttgtc cggtggatag ttgaggatgg aatcccgggt cagctccggc
      961 agcgaactca gggaagcgag gagtttgaaa ggcaggctcg tcttgcccgg attcgggcga
     1021 agcacccccg tgatcgtgaa ccggccgtaa ggggtctcct tgttgccggt ttcaggtcct
     1081 gagggattga tacccgtatc gttgaagctt atcgttttcc cgatcgggtc ttgtccgcgg
     1141 aaaagctggt cggcaatctc ttttgagatc accatggtaa acggcttcgc gagcgcggtg
     1201 gcggtgtctc cccgttcaag ctggaaatcc atgaccctga acagattgtc atcggcgaag
     1261 tagccgcccc cggaggcgat cttgtctttg taaagcaggt cgccgccgat attcttaacg
     1321 aggctggccg ccgcttcgat gccggaatat tgggtccgca gcagcgcccc caatggtagc
     1381 gcggaagttg ccgtccgcat gcccttgccg tcttttcccg tcgtctcgat ccggtaaatg
     1441 cggtccctgt tcttttggaa ccggtcatag cttttttgat cgccgataat gagcatgatc
     1501 agcatgcaga ccgccatggc gacagccagg ccaaaaacgt ttatgaagga gaaggttttg
     1561 tgccggagaa gattcctgaa cgtcacctta aggtaattga caaacatggc gatggtttag
     1621 ggattagacc gccggaccgg tggacgggtt gcaagtggag gcgcgaattg ttggaattat
     1681 ctcaaaatat agtgaaattt acccaaagat gccaggctca tggaatcgcc cccggtccgg
     1741 cccctgatgg cagccggttc gaaggcgcgg tggatctatc gaaagacccg ggacggcaag
     1801 ccctggcacc tgcggggcgt cgatcaggca gccgcggccc gcgccaagca gccgcgaccg
     1861 gccgcgcgaa gtcgcgaccg gcgccaaaca cccgccttgc ggtgaaattc agatttcttc
     1921 acaataaata ccccgctcat gaactggaga ctcatcttcg tcctttcctt gtttggactg
     1981 gcgatggcat ttgcaaccat tacggtgatc ccgacttacc gggagccgtg gttctttctg
     2041 gtcatattcc tgatctgcgc ctattgcatc gcgaaatggg cgcccgggaa attctttctg
     2101 catggattcc tggtcagcct gttgaattgt gtgtggatca ccgcggtgca cattatgtat
     2161 tcgacaacct accttgctca tcatgcgcaa gaagcgtccc agtacagcaa gatgaatgcc
     2221 cagctgggag tcagcccgac ggtagccatg ctgctggtgg gaccggtgat cggcgtcgtc
     2281 tcggggctga tactcggcct gtttgccttt gtcgcatccc ggatggtcaa gagccacctg
     2341 gcgccgctat agcaggccct ttttgaattg atcagctgcg taattggctg ccct
//
LOCUS       DATGKE010000401         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1281813, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000401 DATGKE010000000
VERSION     DATGKE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281813"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1379
                     /locus_tag="VKU83_06955"
     CDS             213..1379
                     /locus_tag="VKU83_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLI93328.1"
                     /translation="MKELKFVNPVRSAAILIILVICFVILSWAQGVIVPLLFAVIFSS
                     MLFPICFRLEAWGCHKGLAAFASVLVAGLLSAVFLGIIISQLVHLAGQAPQFTARISV
                     LADKAENYVSQRFHIEKSAQAVHLHQQLAKLLDNSSAYLSSTMSFTTNMLTQFGLILL
                     FSFFLLYLRVFFLEFFYKVFPSSEKNLIDATMQKIYDVIQSYLLGLLKVIAIIGTLNT
                     IGLWALGIESPLFFGFLGGLLVVIPYIGILIGSALPFIVALITKDSYWYAAGVLAVFL
                     FVHILEGNLITPYVVGSKVSINPLVAIFSLLLFGKLWGLAGLVLALPVTAICKIIFDT
                     LPGFKAVGFLLGKPQKYHFKRYSILHAKLRSRGESADVTITSAVSSAEAPSQNS"
     gene            complement(1487..2023)
                     /locus_tag="VKU83_06960"
     CDS             complement(1487..2023)
                     /locus_tag="VKU83_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLI93329.1"
                     /translation="MALLRDLFVEMLREMYGAEKGQIAALPAMSRAATTDELENLFDE
                     HLAQTEEHVYRLQRVFKMIGDEPGAKTSEAMAGLVREAGGLSAETENGSMTRDVALIA
                     AAQKIEHYEMAVYGTLVQLASNMHLNEVAGLLEQTLREEKEADMQLTEVAERYVNWQA
                     LENENGGGYIRPLAETNR"
     gene            complement(2083..2571)
                     /locus_tag="VKU83_06965"
     CDS             complement(2083..2571)
                     /locus_tag="VKU83_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93330.1"
                     /translation="MTRKDKRAIDMPEVRDIPGQEHVRPPRLGEMADNTASSADEEGE
                     EVLKPLDRAEAGDDDDLEIRGPSNVSPEERELLATADGEPGDDLSLRTGLLDSTDNEG
                     TPLNEKSFGEGRNDEVSGDDLDVPGADQDDRDEAIGEEDEENNDYSLSDNGDDTEINP
                     DL"
     gene            complement(2587..2754)
                     /locus_tag="VKU83_06970"
     CDS             complement(2587..2754)
                     /locus_tag="VKU83_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93331.1"
                     /translation="MKATRLLLAAIGILLLTSLGTGCIPPPPHQHPPKPATPPGGPAA
                     IEHSIILSVNC"
     gene            complement(2891..>4291)
                     /gene="uxaC"
                     /locus_tag="VKU83_06975"
     CDS             complement(2891..>4291)
                     /gene="uxaC"
                     /locus_tag="VKU83_06975"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLI93332.1"
                     /translation="LATEAAQRLYHDYAKGMPVLDYHCHLPPQQIAADHRFANLTQAW
                     LYGDHYKWRAMRTNGVEESFCTGEKPDWEKFQKWAETVPYTLRNPLYHWTHLELQRYF
                     DIGEILNGQTARMIYDSCTAKLQAKEYSVRNLLRKMKVQTVCTTDDPVDSLEYHRQIR
                     EDRFEIPVLPAFRPDNAMNVDHPETFNAYVSKLEAATDLAVADFTDYLDALKKRHDYF
                     GELGCRLSDHGLEEMYAEEFTGSEAATLFNKLRGGRELNDSERRKFKSAMLLHFAEWD
                     WEKGWVQQFHLGALRNNNSRMSRRVGPDTGWDSIGDFSQGRALSRFLDRLDRNDRLAK
                     TILYNVNPADNELIATMAGNFNDGSVPGKLQFGSAWWFLDQKEGMVRQMNSLSNMGLL
                     SRFVGMLTDSRSFLSYPRHEYFRRILCNLFGEEMTNGELPWDMDWVGKVVQDICYYNA
                     RQYFNFQAVAPPARPD"
BASE COUNT          784 a         1333 c         1127 g         1047 t
ORIGIN      
        1 atcttgctga ttgggtggct tcttggcgtt ttcgtttata gtgtgggcgg catgattcac
       61 attctgatcg tgctggccgt aatatccctg atcctcggtg ttatccggag ggcctgaggg
      121 gttctccgtt gtcaatacag gccggtccct cgaccggcta ttttttcttc cgtacgctgt
      181 gtcgcgatcc gccctgaaac gcctatgacg ccatgaaaga gctgaaattc gtcaatccgg
      241 tgcgaagcgc tgccatcctg atcatcctgg taatctgctt cgtcatcctc tcgtgggcgc
      301 aaggtgtaat tgttccgctc ctgttcgccg tcatcttctc gtccatgctt tttcccattt
      361 gctttcgcct ggaagcctgg ggttgccaca aagggcttgc ggcctttgcg tccgttctcg
      421 tggccggact gttgagcgcc gttttcctgg gcatcatcat cagccagctc gtccatctcg
      481 ccggccaggc gccgcagttc accgccagga tctccgtgct ggccgacaaa gccgaaaatt
      541 acgtatccca acgattccat atcgaaaaat ccgcacaagc cgtccacttg catcaacaac
      601 tggcgaagct gctggataac agcagcgcat acctgtcatc gacaatgtcg ttcaccacga
      661 atatgctgac ccaattcggg ctcatcctcc ttttcagttt cttcctgctt tacctccgcg
      721 tcttctttct cgaattcttt tacaaggtct ttccttcttc ggaaaagaac ctgattgatg
      781 cgaccatgca gaagatctac gatgtcatcc aaagttatct cctggggctg ctgaaggtga
      841 tcgccatcat cggaacgctc aataccattg gcctctgggc gctggggatc gagtcgccgc
      901 tcttctttgg ctttctcggc ggcctgctgg tcgttattcc gtatatcggc atcctgatcg
      961 ggtcggcgct tcccttcatc gttgcactca tcaccaagga ctcctactgg tatgccgccg
     1021 gggtgctggc cgttttcctt tttgttcata tactcgaggg aaatctcatt acgccgtatg
     1081 tggtcggctc caaggtcagc atcaatccgc tggtcgcaat cttttcgctg ctgctgttcg
     1141 gcaagctgtg gggtctcgcc gggctggtgc tcgccctgcc ggtgactgcc atctgcaaga
     1201 tcatcttcga taccttgccg ggattcaaag cggttggatt ccttctgggc aaaccacaga
     1261 aataccattt taagcgatat tcgatcctgc acgcaaaact tcgcagccgc ggtgagtccg
     1321 ccgatgtcac gatcaccagc gcggtgtcat cggctgaagc cccctcccag aacagctgac
     1381 cgctcccggc cgggcgccgg cggccgcggc cgcggcccca aaaaagtaag ccatcccgga
     1441 gagccgggat ggcgtccatt aacctaaacc tctatcgcac aaatttttat ctattcgttt
     1501 ctgctaacgg cctgatgtaa ccgccgccat tttcgttttc cagcgcctgc cagttcacgt
     1561 agcgctccgc cacctccgtg agctgcatgt ctgcttcttt ttcttcgcgc agggtctgct
     1621 ccagcaggcc ggcgacctca ttcagatgca tattgcttgc cagctggacc agggtgccgt
     1681 acactgccat ttcataatgc tcgatcttct gtgcagcggc gatcaacgct acatcccggg
     1741 tcatgcttcc gttttccgtt tccgccgata gcccgccggc ctcccggacc aggccggcca
     1801 tggcctcgct ggtcttcgcc ccgggctcat cgccgatcat tttgaagacc ctttggagcc
     1861 ggtatacatg ctcttcggtt tgcgcgaggt gctcgtcgaa caggttttcc agttcatccg
     1921 tcgtcgccgc cctgctcatg gcgggcaagg cagctatctg gcctttctcc gccccataca
     1981 tttcccggag catctcaacg aacaggtcgc gtaacaatgc catcaatccg atcacagaaa
     2041 ataatatgcc acgtcccggg ggcccgtcac cgcgtcaccg cgtcacaagt ccgggttgat
     2101 ctcggtgtcg tctccattgt cgctgagact gtagtcatta ttttcctcgt cctcctcgcc
     2161 gatcgcctca tcccggtcgt cctggtcggc gccggggaca tccaggtcgt cgcccgatac
     2221 ctcgtcgttg cgtccctcgc cgaagctctt ctcattgagc ggggttccct cgttgtcggt
     2281 gctgtccagc aaccctgtcc gcagcgacag gtcgtctccc ggctccccat cggcggttgc
     2341 cagcagttcc cgctcttccg gggaaacgtt ggacggcccg cgaatctcca ggtcgtcgtc
     2401 atcgccggct tcggcccggt cgagcggttt caggacctcc tcgccttctt catcggccga
     2461 ggaggcggta ttgtcggcca tctccccgag gcgcgggggc cgcacgtgtt cctggccggg
     2521 gatatccctg acctcaggca tatctatggc gcgcttgtcc tttcttgtca taacggtttg
     2581 ttttacctaa caattaaccg aaaggatgat cgaatgttcg attgcagcgg gtccgcccgg
     2641 cggcgttgcc ggtttaggcg ggtgctggtg aggcggaggc gggatacacc cggtccccag
     2701 gctggtcagc aataaaatgc cgatcgccgc cagtaataga cgagtagctt tcatccgttt
     2761 tttatcctaa ggggcgcaat aaggatgccg gggaaaaaat ttccggccgg gcaaatgggg
     2821 cgccaaacct ccggacggca aacctccggg cggcaaacct ccggacggca aagcgccggc
     2881 ctcccgcggg ctagtccggg cgcgcaggcg gagcgaccgc ttgaaaatta aagtattggc
     2941 gggcgttgta atagcagatg tcctgtacca cttttccgac ccaatccata tcccagggaa
     3001 gctcgccatt tgtcatctcc tccccgaaca ggttgcaaag gatccgtctg aagtactcat
     3061 gcctcgggta ggacaggaaa ctcctcgaat ccgtcagcat gcccacaaac ctgctcagca
     3121 ggcccatgtt cgataagctg ttcatttggc ggaccatgcc ctccttctga tccaggaacc
     3181 accaggccga accaaattgc agcttacccg gaacggatcc atcgttaaag ttgccggcca
     3241 tcgtcgcaat gagctcattg tcggcggggt tcacattgta caagatcgtc ttcgccagcc
     3301 ggtcgttccg gtccaaccgg tcgaggaacc gggagagcgc ccggccctgg gaaaaatctc
     3361 cgatcgagtc ccagcccgta tccggtccca cgcgccggct catcctgctg ttgttattcc
     3421 tcaacgcgcc gagatggaat tgttgtaccc agcctttttc ccaatcccat tcggcaaaat
     3481 gcaacagcat cgcggatttg aacttgcgcc gctcggagtc gttcaattcc cggccgcccc
     3541 tcagtttatt gaagagggtc gctgcctcgg aaccggtgaa ttcttccgcg tacatctctt
     3601 ccaacccgtg atccgacagc ctgcaaccca gctccccaaa atagtcgtgg cgcttcttca
     3661 gggcgtccag gtaatcggtg aagtcggcga ccgcgagatc cgtggctgct tccagcttgc
     3721 tgacataggc attgaaggtt tccgggtgat ccacgttcat cgcgttgtcc ggccggaaag
     3781 caggcaatac cgggatctcg aaccggtcct cgcggatctg gcggtgatac tccagcgaat
     3841 cgaccggatc atcggtggta cagaccgttt gtaccttcat cttccgcagc aggttccgga
     3901 cggaatattc ctttgcctgc aacttcgcgg tacagctgtc atagatcatt cttgcggttt
     3961 gcccgttaag gatctcgccg atatcgaaat aacgctgcaa ttccaggtgt gtccagtggt
     4021 ataagggatt acgcaacgtg taagggacgg tctcggccca tttttggaac ttctcccagt
     4081 cgggcttttc accagtacag aagctttctt ccacgccgtt cgtccgcatg gcccgccatt
     4141 tataatgatc cccatacagc caggcctgcg tcaggttggc aaaccggtga tcggcggcga
     4201 tctgctgcgg cggcagatga caatggtagt cgagcaccgg cattcccttg gcataatcat
     4261 gatagagccg ctgcgccgct tccgtagcca g
//
LOCUS       DATGKE010000402         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_17556, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000402 DATGKE010000000
VERSION     DATGKE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17556"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VKU83_06980"
     CDS             <1..481
                     /locus_tag="VKU83_06980"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HLI93333.1"
                     /translation="SVDMPIRAAAEYLMDDGFSRKLESRQPLLRLQDNLVLVELSFAV
                     PAINLKDLLFQLQLAGYQPILAHPERYLYFSANKSWYEELRNAGCLFQLNLLSFSGYY
                     GPHSRDLANYLVKKGYVEYLGTDLHHSKQLEQIRRSDVIARTVERLIDSGSIRNMNF"
     gene            495..1121
                     /locus_tag="VKU83_06985"
     CDS             495..1121
                     /locus_tag="VKU83_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLI93334.1"
                     /translation="MKHRFAQYGCGLSAPEDWLNFDASPTLRIQRTPVLNVLLRPKPV
                     FPANVRYGDIIKGLPVKDDELDGVFCSHVLEHLSLEDFRRALRNTYKILKPGGVFRLV
                     LPDLEFYARKYLEDLSGGDALAAMTFIRQSMLGIERRPRGLGGMFRSFLGNSHHLWLW
                     DYPSLSTELAAAGFRNIRRCEYHDGPEAFHAVEEYGRFVDCLAVECYK"
     gene            1334..1834
                     /locus_tag="VKU83_06990"
     CDS             1334..1834
                     /locus_tag="VKU83_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLI93335.1"
                     /translation="MATVEFNQLLVNNAEFLKPFAITLTRDSEAAKDLFQETLYRALA
                     NKDKYNVGTNIKAWLYTIMRNIFINNYRRKAKQNTIFDSTPNDFLLNSAQMAVPGTVE
                     SNLRVKDIQAAIHKLPEIFRNPFLLYFDGFKYHEIADMLQEPLGTIKSRIHFARKLLK
                     TQIQRF"
     gene            1844..>2049
                     /locus_tag="VKU83_06995"
     CDS             1844..>2049
                     /locus_tag="VKU83_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLI93336.1"
                     /translation="MDEKEEVILVDEADQPLGRMEKLEAHRRALLHRAFSVFIFNSQG
                     EMLLQQRAAGKYHSAGLWTNACCS"
BASE COUNT          463 a          561 c          569 g          456 t
ORIGIN      
        1 gtctgtcgac atgcccattc gggccgcggc cgaatacctg atggacgacg gtttttcccg
       61 gaagctggaa agccggcagc cgctgctgag gctccaggac aacctggtgc tggtcgaact
      121 ttcattcgcc gtgccggcga tcaatctgaa ggatctcctg tttcaattgc agctggcagg
      181 ctaccagcca atactggccc atcccgagcg gtatctttat ttcagcgcca acaagagctg
      241 gtacgaggaa ttgcggaatg ccggctgttt gtttcaactg aatctgctat ccttttccgg
      301 ttactacgga cctcactcgc gtgacctcgc gaactacctg gtgaaaaaag ggtatgtgga
      361 gtatctgggg accgacctgc accactcgaa acaactcgag cagatccgcc ggtctgacgt
      421 tatcgcgcgg acggtggaac ggctgatcga cagcggttcg atccgaaaca tgaattttta
      481 agttccaaaa caatatgaag cacagatttg cccagtatgg ctgtggactt tcagcgcccg
      541 aagactggtt gaacttcgat gcatcgccga ctttgcgcat ccaaaggacg ccggtgctca
      601 acgttctcct caggccaaaa ccggtgtttc ccgccaacgt ccggtacggt gatatcatca
      661 agggtctgcc ggtgaaggac gatgagctgg atggggtctt ctgttcgcat gtcctggaac
      721 acctctccct ggaggatttt cggcgggcct tgcgcaatac ctacaagatc ctaaagccgg
      781 gaggggtctt ccggctggtc ttgccggatc tcgaattcta cgcccgcaaa tacctggagg
      841 acctgtccgg tggcgacgcg ctggcggcga tgacctttat ccggcagtct atgttgggga
      901 tcgaacggcg gccgaggggg ctggggggca tgttccgctc cttccttgga aattcccatc
      961 atctgtggtt gtgggactat ccttcgctgt cgacggaact tgctgcggcg ggcttcagga
     1021 atatccggcg ctgcgaatat catgacggcc cggaagcgtt ccacgcggtg gaggaatatg
     1081 gccggttcgt cgattgcctg gccgtcgagt gctataaata gcgggttccg gatcggtgac
     1141 ctcggcgagc cggaatggtg accttcaggg tcagtcctgt ggataaatct cccggcgggg
     1201 atcctggcac gcaaccccgt ttctccgctc caattgccgc tcgtcccaaa tcccgactgc
     1261 ctttctggca ccccagcggc acaggccctt tttttgcagg tacaaagacg gacacaaaga
     1321 atcaaatgtt atcatggcta cagtagaatt caaccagctc ctggtcaaca acgcggagtt
     1381 cctaaagccg ttcgccatca cgcttacgcg ggattcggaa gcagcgaaag acctgtttca
     1441 ggagacgctc taccgggctc tggccaataa ggataaatac aacgtcggta cgaatatcaa
     1501 ggcgtggctg tacaccatta tgcggaatat atttatcaat aattaccgca ggaaagccaa
     1561 gcagaatacc atttttgaca gtaccccaaa tgacttcctg ttaaattcgg cgcaaatggc
     1621 ggtgccgggt acggtggaaa gcaacctgag ggtaaaggat atccaggctg ccatccataa
     1681 actcccggag atcttccgga accccttttt gctctatttc gacggattca aataccatga
     1741 gatcgcggac atgctgcaag agcccctggg gacgatcaag agccggatcc attttgcccg
     1801 gaaactctta aagactcaga ttcagcgctt ttaatctcac tccatggatg aaaaggaaga
     1861 ggtgatcctg gtcgacgagg ccgatcagcc ccttggccgg atggaaaaac tggaagcgca
     1921 ccggcgtgcc ctgctgcacc gggcgttcag cgtcttcatt ttcaattcgc agggtgaaat
     1981 gctgctccag cagcgcgcag ccggaaaata ccacagcgca ggattgtgga caaacgcgtg
     2041 ctgcagtca
//
LOCUS       DATGKE010000403         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1186288, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000403 DATGKE010000000
VERSION     DATGKE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186288"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1076
                     /locus_tag="VKU83_07000"
     CDS             561..1076
                     /locus_tag="VKU83_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HLI93337.1"
                     /translation="MSKIWVPVALLALFVIACSKKSSSGTSLDAADQQFVTSAGYHNV
                     DEINAGYLASTNAADSAVKKFAMKMVDDYGAAHSALILVADSFHFAVVSSPDSAYVLY
                     LNQLSNLKATTFDTTYLRGQINDDQAAISLYQAEQSKGGNAAIKAYANTYLPMIQAHL
                     SAADSLLQAGH"
     gene            1135..1506
                     /locus_tag="VKU83_07005"
     CDS             1135..1506
                     /locus_tag="VKU83_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLI93338.1"
                     /translation="MEQAFPYVTRVDVKFDHLEIVDVGKMVAECTDKWFNQTLTRVND
                     SVVRVGIVEGEYHWHKHEKDDEFFFVLSGRLFVDLEDRTIELNPNQGVTVSRGVLHRT
                     RAPKKTVMLMVETSDVVPTGD"
     gene            1522..>2363
                     /locus_tag="VKU83_07010"
     CDS             1522..>2363
                     /locus_tag="VKU83_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93339.1"
                     /translation="MIRRVLLFALLAFSAADCHAGGPGKVALPQSLRHFIRSIRFAAL
                     GDLQPRISVSPVEENITKVTLVYNLPREIRQDDWRVQIVPAFRAGFHWAPLLTPASDN
                     IIAQHVFRSPALIVAGNGREVSVVPDLDWVGRPPEVPWYMDMDAPANTLTLGWSLSAV
                     SAHTEYVRKKGLLITPGKMEFGFYLITGMTAGEPSPWRSTERFLWHKWGYPLYESGEP
                     LSSNLETYVDRTYHWAFDSWRKVVWQQFRLNGKEVGAPVFIVNVTQSPDYPGPATERE
                     FRSI"
BASE COUNT          544 a          655 c          659 g          505 t
ORIGIN      
        1 ccagcagctc caggccagag ttggcgacct gcacagggat gccggactgt tgctcgaact
       61 ccagccgaaa aatcaggcga tcatcctcgt cgtcatcgac catcattaag tacgtattca
      121 gtcccgccat aaacgtccga ataatgacag attttcgctt gctgatagca gcctgtcgct
      181 aatttaatat ctccaatgcg catttgactt aaatgtgcga aaatttaatt tctggctaaa
      241 acccatgaca aatcagtatg gaaaatattc ctcaaacctt taaatcccgg ctaagcgtgc
      301 agtcggtttc atggcaaggc cagggctgtt aacccattca gcctctggcc cttacaggga
      361 aatgatcatc gacaggcaga agggagggag cggtaccgcc ttcccatgct ccttggaaat
      421 gcctcagcct cgaaaggccg ggaatggacc ggagcaggtt gtcgtattgc agcaaccatg
      481 ccagaattag gatggcgtgg ctttacgcag gcaggaccta agattagccc gaaaattgca
      541 agtcatgaca aaaaggaatc atgagtaaaa tttgggtccc ggtggcgctg ctggcacttt
      601 tcgtaatagc ctgctcaaaa aaatccagtt caggcacgtc gttggacgcc gccgatcagc
      661 aattcgtcac gagcgcgggt tatcataacg tcgatgagat caacgcgggt tacctggcgt
      721 caaccaacgc cgccgattcg gctgtgaaga agtttgccat gaagatggtg gacgactacg
      781 gggccgcgca ctccgcgctt atcctggttg ccgatagctt ccattttgcc gtggtttcca
      841 gccccgactc cgcctacgtg ctgtacctca accaactcag caacctcaaa gcgacaacct
      901 tcgatactac gtatcttcgc ggtcaaatca atgatgacca ggccgccatc tctctttacc
      961 aggccgagca gagcaaaggc gggaacgcgg cgatcaaggc ctacgcgaat acctacctgc
     1021 cgatgatcca ggcacacctg agcgcggccg acagcttgct ccaggccggg cattagccac
     1081 actcaaccgg ttttagcgga aatttcggta tattcagggg aatcaacctg cgatatggaa
     1141 caagcatttc cttatgttac gcgcgtcgac gtgaagttcg atcatctcga gatcgtcgac
     1201 gtcggcaaga tggtggccga gtgcaccgat aaatggttca atcaaacgct cacccgggtc
     1261 aacgacagtg tcgtccgagt cggcatcgta gagggcgaat atcattggca taaacatgag
     1321 aaggatgacg aattcttttt tgttttaagc ggccgccttt tcgtggacct cgaggaccgg
     1381 accatcgagc tgaatcccaa ccagggagtg accgtaagtc gtggcgtcct gcaccggacc
     1441 cgggctccga aaaagacagt gatgctgatg gtggaaacca gcgatgtggt gccgacagga
     1501 gattagaaga tttcaggttc tatgatcaga cgagttctgt tatttgccct gctggccttc
     1561 agcgctgccg actgccacgc aggcgggccg ggcaaggtgg cgctaccgca atcgctacgg
     1621 cattttatcc gctccatccg cttcgctgcg cttggggacc ttcagccgcg gatatcggta
     1681 tcgcccgttg aagagaacat taccaaggtg acgctggtct acaatctgcc ccgggaaatc
     1741 cggcaggatg actggcgggt acagatcgtg ccggcattcc gcgccggttt ccactgggcg
     1801 cccctgctga ctcccgcctc ggacaatatc attgcccagc acgtgttccg ttcccccgcg
     1861 ctgatcgtgg caggcaacgg ccgcgaggtt tcggtagttc ccgacctgga ttgggtgggc
     1921 cggccgccgg aggtgccgtg gtatatggac atggatgcgc cggccaacac ccttacactc
     1981 ggctggagtc tgtcggccgt gagcgcgcat acggagtacg tccggaaaaa aggactgctg
     2041 attacccccg gaaagatgga gttcggattt tacctgatca cgggcatgac ggccggcgag
     2101 ccaagcccct ggcggtcgac cgaacgtttc ctctggcata aatggggata ccccttatac
     2161 gaaagcggtg agccgctatc ctcgaacctc gagacctacg tcgaccggac ctatcattgg
     2221 gcgtttgact catggcggaa agtggtatgg cagcaattcc ggctgaacgg taaggaagtg
     2281 ggggcgccgg tttttatcgt gaacgtaacg caaagcccgg actacccggg cccggctact
     2341 gagcgggagt tccggtctat ctg
//
LOCUS       DATGKE010000404         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_208458, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000404 DATGKE010000000
VERSION     DATGKE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208458"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1507
                     /gene="fumC"
                     /locus_tag="VKU83_07015"
     CDS             107..1507
                     /gene="fumC"
                     /locus_tag="VKU83_07015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLI93340.1"
                     /translation="MEYRIEKDTMGEVKVPVDAYYGAQTQRSVLNFPIAQDINRMPKE
                     IIRAFAYLKKAAALTNLDAGVLSQEKADLIGKVCDEILSGALDAYFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLAGGKLTDKEKTLHPNDDVNKSQSSNDTFPTAMHIAAYQMLAA
                     TTIPGIEALRDTLAAKSREYMKVVKIGRTHFMDATPLTVGQEFSGYVSQLDHGLRAIR
                     NTLPHLSELALGGTAVGTGINTPPGYAESVARHIAALTGLPFVTAANKFEALAAHDAI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     LTMIAAQVLGNDVAINVGGATGHFELNVFKPVMIYNFLHSARLIGDGCSSFNAHCAVG
                     IEPIEANIRRHVDNSLMLVTALNTRIGYYKAAEIAQKAHREGTTLREMAVKLGYVTPE
                     QFDEWVKPEAMVGKLG"
     gene            complement(1608..1955)
                     /locus_tag="VKU83_07020"
     CDS             complement(1608..1955)
                     /locus_tag="VKU83_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI93341.1"
                     /translation="MNIYVANLSFAVQDEDLREFFAPYGEVTSAKVIMDKVSGRSRGF
                     GFVEMSDDEAARKAIAELDGATVEGRAIKVNEAKPKEEKPSFNRGGGGFGGGRGGYGG
                     GNRGGDRGGYGRY"
     gene            2141..2782
                     /locus_tag="VKU83_07025"
     CDS             2141..2782
                     /locus_tag="VKU83_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLI93342.1"
                     /translation="MNPHSFLEPHSRAIRLWHWTFVLFVSATIGIVILASTLLRTGNN
                     VPLVEHQLAEKGVSISEDQARSVAHSFNDELWDVHRWLGYGLSLLLVFRWFIERAQPR
                     AERLNYKVRRAAGFRSVIPIEQDQSRHYLVVKSIYLVFYGVFVAMALSGLVQAFDDVP
                     RLDEYQDTAKQLHSFLQWPIYFFIFIHLVGVIRADLGYHKGIVSGMIHGQKLG"
BASE COUNT          671 a          808 c          746 g          583 t
ORIGIN      
        1 aaggcaaatg acaaggaaat aaagcagtat ggtgtttcgc atagcaataa aaatagacaa
       61 aatccggcca ttccagccta cctttacggc ccgaaaacgt caaaaaatgg aataccgaat
      121 cgaaaaagac acgatgggag aggtcaaggt gcccgtcgac gcctactacg gcgcccagac
      181 gcaacggagc gtgctgaatt tcccgatcgc ccaggatata aacagaatgc cgaaagagat
      241 catccgcgcc ttcgcctatc tcaagaaggc tgcggccctc accaatctcg atgccggcgt
      301 cctgtcacag gaaaaggctg acctcatcgg caaggtctgc gacgagatcc tcagcggcgc
      361 cctggatgcg tattttccac ttgtcgtgtg gcagaccggg agcggcacgc aaagcaatat
      421 gaacgtcaac gaggtcatcg cctaccgcgg ccacgtactc gccggcggca agctgacgga
      481 caaggagaag accctgcacc ccaacgatga cgtcaacaaa tcccagtcgt ccaacgatac
      541 gtttccaacg gccatgcaca tcgcggcata ccagatgctg gcagcgacga cgatcccggg
      601 cattgaggcg ctgcgtgata cgcttgccgc aaaaagcagg gagtacatga aggtcgtcaa
      661 gatcggccgc acccatttca tggatgcgac gccgctcacg gttggccagg agttcagcgg
      721 ctatgtgagc cagttagatc atgggcttcg cgccatccgc aatacactcc cgcacctctc
      781 cgaacttgcg cttggcggca ccgccgtcgg gaccgggatc aatacgcctc cgggctacgc
      841 cgaaagtgtg gcgcgccata tcgctgccct caccggcctg ccgttcgtca cagccgctaa
      901 taaattcgag gccctcgccg cgcacgacgc tatcgttgaa gcacatggcg cgctgaaaac
      961 cgtggccgtc agcctgatga agatcgccaa tgacatccgc atgctgtcct ccggaccgcg
     1021 gagcggcatc ggcgagatcc acatccccga caatgaaccg ggctcttcga tcatgccggg
     1081 caaggtcaac cccacgcaat gcgaggcgtt gacgatgatc gccgcgcagg tactgggcaa
     1141 tgatgtggcg atcaatgtgg gtggggcgac ggggcacttc gaattgaatg tttttaagcc
     1201 ggtgatgatc tacaactttc tccacagcgc gcggctgatc ggtgacggct gcagcagctt
     1261 caacgcgcat tgcgctgtcg gcatcgagcc gatcgaggcg aatatccgca ggcatgttga
     1321 caattcgctg atgctcgtta cggcgctcaa caccaggatc ggctactata aagccgccga
     1381 gatcgcgcag aaagcgcatc gggaaggcac gaccctcagg gagatggcgg ttaagctggg
     1441 atatgtcacg ccggagcaat tcgatgagtg ggtaaagccg gaggcgatgg tcggcaagct
     1501 tggctgaggc cttcgggcat gaggccttcg ggcatgaggc cttcgggcat gatataaatc
     1561 aaaaagaccg gctgtcgccg gtctctttac gtgggtatac aaaacgatta atatcttccg
     1621 taaccgccgc gatcaccacc acggttgccg ccgccataac cgccgcgacc gccgccgaag
     1681 ccaccgccgc cacgattgaa tgagggtttt tcttccttgg gcttggcctc gttgaccttg
     1741 atagcccggc cttccaccgt ggcgccatcc agctcggcga tagccttgcg ggcagcttca
     1801 tcatcggaca tttcaacgaa accgaacccg cgggagcggc cgctgacctt gtccataatt
     1861 actttggcag aggtgacttc cccgtaaggg gcgaaaaatt cccttaaatc ttcatcctga
     1921 acggcgaagc tcagatttgc aacatagatg ttcatacgaa actctaaaat ttaatgataa
     1981 atgaactgag aaggcaaaaa acgaatacta actagatgcg aggcagataa ttaatgatta
     2041 cggttgctaa ctcaaattac tccacaaata tatacattat tcacggattg acaaatatat
     2101 ttattacttt catgcgtcga gcgattggca gcaatcagtt atgaatccac attccttcct
     2161 cgaaccgcat tccagggcga tccggctttg gcattggaca ttcgtcctat ttgtgagtgc
     2221 gaccatcggg atcgtcatcc tggcctccac cctccttcga accggcaaca atgtacccct
     2281 cgtggaacac cagctcgccg aaaaaggcgt cagcatcagc gaggatcagg cccgttcagt
     2341 agcccactca tttaatgatg aactttggga cgtccaccgc tggctcggct atggcctttc
     2401 attattgctc gtgttccgct ggtttatcga gcgggcgcag ccgcgtgccg aacggctgaa
     2461 ttataaggtt cgcagggctg ccggtttccg cagcgtcatc ccgatagaac aggatcaaag
     2521 ccgccactac ctggtggtaa agtccatcta tctcgtcttc tacggcgtct ttgtggcgat
     2581 ggccctaagc gggctcgtcc aggcattcga cgatgtcccg cgtctcgatg agtaccagga
     2641 caccgcgaag caactccact ccttcctcca atggccgatc tacttcttta tatttatcca
     2701 cctggtggga gtcattcgcg cggatcttgg ttatcacaag ggaatagtat cgggcatgat
     2761 ccatggccaa aaactcggtt aagcgggcta tttgtcgtat cttaccct
//
LOCUS       DATGKE010000405         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_676181, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000405 DATGKE010000000
VERSION     DATGKE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676181"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VKU83_07030"
     CDS             <1..499
                     /locus_tag="VKU83_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HLI93343.1"
                     /translation="GVGYLSPADAISYGCSGPTARGSGVSCDIRKLYPYEVYADVHFE
                     EVKAAGGDCYARYLVRMQEMRESVSIIEQLIDNIPEGDFQAKTKAVLKLPKGEYYSRV
                     ETARGEFGVYIISEGGTTPYRIKFRSPGFSNLSALDHMARGHKIGDLVAIMGTLDLVI
                     PDIDR"
     gene            542..1618
                     /gene="nuoH"
                     /locus_tag="VKU83_07035"
     CDS             542..1618
                     /gene="nuoH"
                     /locus_tag="VKU83_07035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HLI93344.1"
                     /translation="MLSIENITESIDTWLNATFSPGLAVLLEGVIIGLCAIGLFAVLG
                     LVLVLMERKVSAYMQVRLGPNRVGPGGSLQTLADTLKLVLKEGLTPDGADKFLFNLAP
                     YLVICVAMLILIPLAFARGTQIWDINIGVLYISAVSSLSVIGILMAGWASNNKYSLLG
                     AMRSGAQIVSYELSAGISVISVVVLTGSLRISDIIASQQDGWWLFKGHIPAIISFVIF
                     IIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNVFIVCGIGATLFL
                     GGWMPLHIGHWAGFNRVMDYIPSSLWFVAKTFLLIFVIMWFRWTFPRLRIDQLLNLEW
                     KYLLPISLLNLLLMTWIAIEGWYF"
     gene            1621..2097
                     /locus_tag="VKU83_07040"
     CDS             1621..2097
                     /locus_tag="VKU83_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HLI93345.1"
                     /translation="MSVKKYISDVLGAIRSLLKGMRLTGYYFFHHREIITQQYPDNRE
                     EMKLPDRFRGEVVMLHDEKNEHACTGCTACELACPNGTIKIITKFDTTPEGKRKKALD
                     TFIYHLELCTMCNLCIEACPTDAIKMAQNFEHSVYDRSQLTKKLNKPGSKIREGVE"
     gene            2097..2657
                     /locus_tag="VKU83_07045"
     CDS             2097..2657
                     /locus_tag="VKU83_07045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLI93346.1"
                     /translation="MSASAIVFYLLSFFILASALLAVTSRKIFRSAIWLLFSLTGIAG
                     LYFWLQMNFVAAVQIVVYVGGIVVLIIFSIFLTQQTGKDLPLPLRGRAFFSVLAACAG
                     FILACAVLLTQGFSSGLPGGQAPAGGVTAGGDQAVVGSVGDIGSQLLSTSEHGYVLPF
                     EVVSILLLAAMISCIVIAMKTKPESK"
     gene            2654..2968
                     /gene="nuoK"
                     /locus_tag="VKU83_07050"
     CDS             2654..2968
                     /gene="nuoK"
                     /locus_tag="VKU83_07050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HLI93347.1"
                     /translation="MNQVPLIHILFVSTALFFIGMYGLFTRRNLITMLMSVELILNSV
                     NINFVAFNRYLYPDRLDGVFFTLFIIAIAAAEAAVAIAIIINLYRNFHSIDVEAADEM
                     KF"
BASE COUNT          619 a          783 c          858 g          747 t
ORIGIN      
        1 gggcgtcggg tatctttcgc ccgcggatgc catttcctat ggctgttccg gcccgacggc
       61 aaggggaagc ggcgtaagtt gcgatatccg gaagctctat ccgtacgagg tgtatgcaga
      121 cgtccatttc gaagaggtga aggccgccgg cggcgattgc tacgcgcgct acctggtccg
      181 gatgcaggaa atgcgcgagt ccgtttccat catcgagcag cttatagata atatccccga
      241 aggtgatttc caggcgaaga ccaaggcggt actgaaattg cccaaaggag agtactacag
      301 ccgggtggag acggccagag gggaattcgg cgtttacatt atcagcgagg gtgggacaac
      361 gccgtaccgg ataaaattcc gcagcccggg attctcgaac ctttccgccc tcgatcacat
      421 ggcccgagga cacaagatcg gggacctggt ggccattatg ggcacgctgg acctcgtgat
      481 tcccgatatt gacaggtgac tgggggcggg ctgaacggag gaacctgagg caaaaatgaa
      541 gatgctgagc attgaaaata taacggagtc cattgacacc tggctgaacg ccaccttcag
      601 tcccggcctt gccgtcctgc tggaaggcgt gatcattggg ttgtgtgcca tcgggctctt
      661 tgcggtactg ggattggtcc tggtcctgat ggagcgaaag gtttccgcgt acatgcaggt
      721 gcggctcggg ccgaaccggg tcggccctgg cggaagcctg caaacccttg ccgacacctt
      781 aaaacttgta ctgaaggagg ggctcacgcc ggatggcgcc gacaagttcc tgttcaacct
      841 ggccccttac ctcgttatct gtgtcgccat gctgatactc atcccgctgg ccttcgcgcg
      901 cggaacgcag atctgggaca ttaatatcgg ggtgttgtat atctctgccg tttcttcgct
      961 ttcagtaatc gggatcctga tggcgggatg ggccagcaat aacaagtatt cgctgcttgg
     1021 agcgatgcgt agcggcgcgc agatcgtgag ctatgagctg tcggcaggca tctcggtcat
     1081 ttcggtggtg gtgctgacgg gcagcctgcg gatttcggac atcattgcct cgcagcagga
     1141 tggttggtgg ctgttcaaag gccatattcc ggcaatcatt tccttcgtga tctttatcat
     1201 cgccgtcacg gcggagacca accgggcgcc tttcgacctg gccgaggcag aatccgagct
     1261 caccgcgggt ttccataccg agtactcggg gatgaaattc gccctctttt tcctggctga
     1321 atatgtgaac gtttttatcg tttgcggaat cggcgccacc ttattcctgg gtggatggat
     1381 gcccctgcat atcggtcact gggccggatt caaccgggtg atggattata ttccttcctc
     1441 cctttggttc gtggctaaaa cttttttgct gatcttcgtc atcatgtggt tccgctggac
     1501 tttcccccgg ctgcggatcg accagttatt aaaccttgag tggaagtatc tgctgccgat
     1561 cagcctgctg aacctgctct tgatgacctg gatcgctatc gaaggatggt atttttaaga
     1621 atgtcagtaa agaaatacat atcggatgtc ctgggcgcca tacggtcctt gttaaaagga
     1681 atgcggctga caggctatta ttttttccat caccgggaaa taatcactca gcaatatccc
     1741 gacaaccggg aagaaatgaa gctccccgac cggttccggg gagaggtggt catgctccat
     1801 gacgagaaga acgagcacgc ctgcaccggt tgtaccgcct gcgaactggc ctgtccgaac
     1861 ggaaccatta agatcattac gaagtttgat acgaccccgg aggggaaaag gaagaaagcg
     1921 ctggatactt ttatctacca cctggagctt tgcacgatgt gcaatttatg catcgaggct
     1981 tgcccaacgg atgccatcaa gatggctcaa aatttcgaac acagtgtgta tgaccgaagc
     2041 cagctgacaa aaaaattaaa taaacccggt tctaaaatca gggagggcgt tgaataatga
     2101 gcgcatcagc catcgtcttc tacctgcttt cgttttttat cctggcttct gcgctgctgg
     2161 cggtgacttc caggaagatt ttccgttcgg ccatctggct attgttctcc cttacgggca
     2221 ttgccgggtt atatttctgg ctgcagatga attttgtggc cgcggttcag atcgtcgtgt
     2281 atgtcggcgg aattgtcgtc ctgatcattt tttccatttt tcttacccaa caaacaggca
     2341 aggacctccc ccttcccctg cgtggcaggg ctttcttctc ggtcctggcg gcctgtgccg
     2401 ggttcatcct ggcttgcgcc gtattgctga cccagggctt ttcgtccggc ctgcccggcg
     2461 ggcaggctcc ggccggcggt gtgacagcag gcggagacca ggcggtcgtc ggaagcgttg
     2521 gggatatcgg gtcgcagctg ctcagcacct cggaacacgg gtacgtattg ccctttgagg
     2581 tggtcagtat cctgctgctg gcggccatga tcagctgtat cgttatcgcc atgaaaacca
     2641 aacccgaaag caaatgaacc aggtgcccct gatccatatt ctgttcgtga gcacggcgct
     2701 ttttttcatc gggatgtacg gcctgttcac gaggcggaac ctgattacca tgctgatgtc
     2761 cgtggagctg atcctgaaca gcgtcaacat caattttgtg gcgtttaacc ggtacctgta
     2821 tccggaccgg ctggacggcg tattcttcac tttgttcatt attgccatcg cggctgccga
     2881 agcggcggta gcgatcgcca ttattattaa cctataccgg aattttcatt cgatcgacgt
     2941 ggaggcggcg gacgagatga aattctgaca atcgcgcgta tgccggacac tgcttttttg
     3001 ctgctga
//
LOCUS       DATGKE010000406         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1004555, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000406 DATGKE010000000
VERSION     DATGKE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004555"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VKU83_07055"
     CDS             complement(<1..983)
                     /locus_tag="VKU83_07055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLI93348.1"
                     /translation="MPAGQQIVPPAPAAPAGPDRQSPRDSDTVTASGRQRSLSQAVRE
                     WFARAFNTAVLQKNRLAWVDYLRGIAILLVVYRHVLLGIQNSGYVLPAAYLNANIMFF
                     SFRMPLFFILSGIFISGSLARKSLGRIVYSKFELLLYPYLIWATVQITIQIFASHYTN
                     ANRTAFDYLYIFYQPEKIDQFWYLPALFNSTIVYLFTKTKLNLRGWMQLLLGLTLYFL
                     SPLCSQVSMMSNWMEFYIFFAIGDTMSTFFFREKVQAFLNHRFTFPALFPVFLLTQFY
                     YLHLYLTSPNNIVPLYLFLPIALFGCLTMCTLAYRLQSWNVLRFLRVLGYHS"
     gene            complement(983..1765)
                     /locus_tag="VKU83_07060"
     CDS             complement(983..1765)
                     /locus_tag="VKU83_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93349.1"
                     /translation="MKSSSTQLLRRTSPITSAALATLLFASAAMGQRPTGGRSRVDSP
                     LVSRNARPATPAPSVNQDSLIQERLVQLALAGPTYSVTGHQIKFAEYNLSRARRTWLN
                     LLSISADYNDQTFASHSATYGYVYPKYFFGITIPIGLFFTMGPDIHKEQENVAIAHDN
                     QEELARTIRAQILAKYAQYKAYGLLIANQTNVVDDEEALRKQVEKKFQDGSITIEQYN
                     DANKAYGDDLTKKLNLQLQQDLLKIDIERMIGVNLESVLKTR"
     gene            complement(1782..>2858)
                     /locus_tag="VKU83_07065"
     CDS             complement(1782..>2858)
                     /locus_tag="VKU83_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI93350.1"
                     /translation="KTVTSDASKAAALQSLMKEQELANKEYLDAKQRFNDAYDIMASS
                     VNNFRLVLAGQPPLEPESSKRILIVGLAGIFVFVISVLVITLMTYMDSSLKTPVIFAK
                     TVNLKLLSMVNYTNLRNKSLSELIIEPAHTAASGRRGRKAIDSKGTAENNRQNVFRES
                     IRKLRYEVENSGKRVFLVASTKKGEGKTTLIQMLAFSLSLNKKRVLIIDTNFSNNDLT
                     VQLDADPILEKIHPDRTNPRTLLEQVRNAAKNVIGESVYVIGSQGGDYTPSEILPREN
                     LLQQLPALKAEFDYIFLEGPPLNDFTDAKELAQYVEGVIAVFSSHHVIRQIDKQSMTF
                     FHELNGKFCGAILNMVDLEDVNAI"
BASE COUNT          623 a          692 c          887 g          656 t
ORIGIN      
        1 gaatggtagc ccaggacgcg caggaagcga agcacattcc aggactgaag acgataagcc
       61 agggtgcaca tcgtcaggca gccgaaaagc gctatcggta gaaaaaggta gagcggcaca
      121 atattgttgg gactcgtcag gtaaagatgc aggtaataaa actgtgtcag gaggaacacc
      181 gggaataggg ctggaaaagt gaagcgatgg ttcaggaacg cctgcacctt ctcccggaag
      241 aagaatgtcg acatcgtatc gccgatcgca aagaagatat agaattccat ccagttggac
      301 atcatgctga cctggctgca caggggcgac aggaagtaaa gcgtcagccc aagcagcagc
      361 tgcatccagc cgcgcaggtt cagtttcgtc ttcgtaaaaa ggtatacgat ggtggaattg
      421 aaaagcgcgg gcaggtacca aaactggtcg atcttttccg gctggtaaaa gatatagaga
      481 tagtcgaaag cggtgcggtt ggcattggta tagtggctcg caaagatctg gatggttatc
      541 tgtaccgttg cccagatgag atacgggtag agcagcagct cgaacttgga atacacgatc
      601 cggcccaggc tcttcctggc aagactgccg ctgataaaaa tgcctgagag gatgaaaaag
      661 agcggcattc gaaagctaaa aaacatgata ttggcattca ggtaagcggc cgggagtaca
      721 taaccgctgt tttggatgcc gagcaggacg tgccggtaga ccaccagcag gatggcgatg
      781 ccccgaagat aatcgaccca tgccaggcgg ttcttttgga gcacagcggt gttgaaggcg
      841 cgggcaaacc attcgcggac ggcttgcgaa agggagcgtt gccggcctga cgccgttacg
      901 gtgtcgctgt cgcgcgggct ttggcggtca gggccggcgg gcgccgcggg agcgggaggc
      961 actatttgtt gtcctgcggg catcatcgcg tttttaatac ggattcgagg ttgacgccga
     1021 tcatcctttc gatgtcgatc ttcagcaggt cctgttgcag ctggaggtta agttttttgg
     1081 tgaggtcgtc gccataagcc ttattggcgt cgttgtattg ttcgatcgtg atggagccgt
     1141 cctggaattt cttttccact tgtttgcgca gcgcctcctc gtcatcgacg acgttggtct
     1201 gattggcgat gagcagaccg taagccttgt actgcgcata tttggcgagg atttgcgccc
     1261 ggatcgtccg ggcaagttcc tcctggttgt cgtgggctat cgccacattt tcctgttcct
     1321 tatggatatc cggtcccatg gtgaaaaaca gcccgatggg aatggtgatg ccgaagaaat
     1381 attttgggta aacatagccg taggtagcgg agtggctcgc gaaggtttgg tcattgtagt
     1441 ccgccgagat gctcagcagg ttgagccagg tacgccttgc ccgggagagg ttgtattccg
     1501 caaatttgat ctgatgcccg gtaacggagt aggtggggcc ggcgagcgca agttgcacca
     1561 gcctttcctg gatcagggag tcctggttca ccgacggcgc gggagtggcg ggacgcgcgt
     1621 tcctcgaaac taacgggcta tcgacccggc tgcgtccccc cgtcgggcgc tgacccatcg
     1681 cggccgaggc aaagagcagg gtagcaagcg cggcagaagt tattggggag gtgcggcgaa
     1741 gaagctgggt tgatgatgac ttcataaggt taataagtaa tttaaatggc gttgacgtct
     1801 tcaaggtcca ccatgttcag gattgccccg cagaatttcc cgttcagctc atggaaaaaa
     1861 gtcatggact gcttatctat ttgccgaatg acatgatgcg acgagaagac cgcgatgaca
     1921 ccctccacgt attgcgccag ttccttggcg tcggtaaaat cattcaaggg cggaccttcc
     1981 aggaagatat agtcgaactc cgctttcagg gccggcaact gttgcaggag gttttcgcgc
     2041 ggaaggatct ccgacggcgt ataatcgccg ccctgggaac cgatgacata gaccgattcg
     2101 ccgatcacgt tcttggcggc gttgcggacc tgttccagca gggtccgcgg gttggtcctg
     2161 tccggatgga tcttttcgag gatcgggtcg gcgtccagct gcacggtgag gtcgttgttg
     2221 ctgaaattcg tatcgatgat cagtacgcgc tttttgttca ggctcaggct gaaggccagc
     2281 atttggataa gcgtcgtctt gccctcccct ttcttcgtac tcgcgaccag gaacacccgc
     2341 ttgccgctgt tctccacctc gtagcgcagt ttgcggatgg attcgcggaa gacgttttgg
     2401 cggttgttct ccgccgtgcc tttgctgtcg atcgctttcc gcccgcggcg gccggaggcc
     2461 gccgtgtggg ccggctcgat gatcagttcg gaaagggact tattcctgag gttcgtataa
     2521 ttgaccatgc tgaggagctt caggttgact gttttggcga agatgaccgg cgttttgagg
     2581 gaagagtcca tataagtcat cagggtgatg acgagcaccg agattacgaa tacaaagatg
     2641 ccggcgagcc cgacgatcag gatccttttc gatgattccg gctccagcgg cggctgcccg
     2701 gcgaggacaa gccggaaatt attgacagag gaggccatga tatcgtaggc gtcgttaaat
     2761 cgttgtttgg cgtcgaggta ttccttattg gcaagttcct gctccttcat cagcgactgg
     2821 agagccgccg ccttggacgc atccgaggta acggtctt
//
LOCUS       DATGKE010000407         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_13002, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000407 DATGKE010000000
VERSION     DATGKE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13002"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VKU83_07070"
     CDS             <1..465
                     /locus_tag="VKU83_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HLI93351.1"
                     /translation="KKKAAFEYRLRWDSVRYEPGELKVLTYKDGKPWATDVIRTAGNA
                     AGLQLDADPGVIKADGCDLSFITVRVVDSKGVVVPTADNKLTFAISGPGEIVATDNGN
                     PADLVSFASPGRAAFSGLALVIVQAKPGTAGTITVKASSDGLPSAEIVIQCK"
     gene            699..1397
                     /locus_tag="VKU83_07075"
     CDS             699..1397
                     /locus_tag="VKU83_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLI93352.1"
                     /translation="MADFCLGVDEVTQKEWTAVMGYNPSHFRGDNLPVEMVSWYDCID
                     YCNRRSVKEGLRPYYTIDKHSKDPDNTNELDSIKWKVTINSGANGYRLPTEMEWAYAA
                     EGGQLSKGFIYSGSDSIDDVAWYWKNSGDNDLTGSWSWSAVQQNNNKTQPVGRKAPNE
                     LGLYDMSGNVREWCWDSNGNDGTGQPPGRIWKGGGWMGADFCCASAFRAAYQANGKGP
                     DQGLRVGRDAASSG"
     gene            1459..2229
                     /locus_tag="VKU83_07080"
     CDS             1459..2229
                     /locus_tag="VKU83_07080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLI93353.1"
                     /translation="MVLPIGDDNRDRTTTPIVNYLLIGFNIFVFIFWQQWGHNVQLTY
                     SYATVPGEILTGHDIVTHSKILTDPYTGKQMVLPGLGVTTIPVFFTLFTSMFMHGGLL
                     HLAGNMLFLFVFGDNVEDAMGHAKYLVFYLLCGMLAGLSHVFATFFLGQSLLIPSLGA
                     SGAISAVLAAYVRLFPGKRVDLWFFLFIVPVPAFVVVGIWFIFQVIDGLGMLGGQQTG
                     DVAYAAHIGGFLFGLLLTRFFAGGYLARRAYRRRAGQW"
     gene            complement(2258..>2524)
                     /locus_tag="VKU83_07085"
     CDS             complement(2258..>2524)
                     /locus_tag="VKU83_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HLI93354.1"
                     /translation="IFVPNAFTPAAGSNNVFRPICMGISSLDFFHVYNRWGQLVFSTS
                     QIGQGWDGRINGQLQGANAYLWMLKGTDYTGRSIAKKGTVVLIR"
BASE COUNT          570 a          597 c          747 g          610 t
ORIGIN      
        1 aaaaagaaag cggcattcga ataccggttg cggtgggata gcgtacggta cgaaccggga
       61 gagctgaagg tattgacgta taaagacggc aagccctggg cgaccgatgt catccggacc
      121 gctgggaatg ccgccggatt gcagctcgat gcggatccgg gcgtgatcaa agccgatggc
      181 tgcgatctgt cgttcatcac cgttcgcgtg gtggatagta aaggtgttgt ggtgccgacc
      241 gccgacaata agctcacgtt tgcgatctcc ggacccggcg agatcgtcgc aacggataat
      301 gggaatcctg ccgacctggt ctccttcgct tcacccgggc gggcggcgtt tagcgggctg
      361 gcattggtga tcgtacaggc gaaacctggt acggccggaa cgatcacggt gaaggcgtcg
      421 tccgatggct taccaagtgc ggagatcgtc attcaatgca agtaatcgat tacactcggt
      481 tcctataact atcagatact ggctttggag gcgtacagcg gaaagagcgg atatataaac
      541 gcgaccgcgt ggggccaata agcaaatgca cgttaccggt aatcatcgtg ttcgtttttg
      601 ccggttcctg ttcaacaaaa gccccggcca gcttcgtaag gataaaaggc ggggcttttg
      661 tcaatacaag atctacgtgg tacgggaaaa gtatccgcat ggcggacttt tgtctcggcg
      721 tggatgaagt gacccaaaaa gaatggacag cggtaatggg gtacaacccc tcccatttcc
      781 ggggcgacaa tctgccggtg gagatggtga gctggtatga ctgtatcgat tattgtaaca
      841 ggagaagtgt aaaggaaggg ttgagaccat attataccat tgacaagcat tcaaaggacc
      901 cggataatac caatgaactg gattccatca aatggaaagt gaccatcaac agcggcgcca
      961 atggttatcg tttgcctacg gaaatggagt gggcgtatgc tgcagaaggg gggcagctga
     1021 gcaagggctt tatctacagc ggaagcgata gcatcgacga tgtagcatgg tattggaaga
     1081 attccggcga taacgacctg actggatcct ggtcctggtc tgcggtacag caaaataaca
     1141 ataaaaccca accggtcggc aggaaagcgc ccaatgagct ggggctgtac gatatgtcgg
     1201 gtaatgtccg ggaatggtgc tgggattcga atgggaacga cggtaccggg caaccgccgg
     1261 gccggatctg gaaaggcggc ggctggatgg gggccgattt ctgttgcgct tccgcgtttc
     1321 gtgcggcata tcaggcaaat ggcaaggggc ccgaccaggg attgcgggta ggtcgcgatg
     1381 cagcgtcatc cgggtgaatt tgcggacctc cactcttttt ccgccaaatt atttttttgg
     1441 gggtaatttt aagcaaaaat ggttttacct atcggtgacg ataacaggga taggacaacc
     1501 acccccatcg tcaattacct gcttataggg tttaacatct ttgtatttat cttttggcag
     1561 caatgggggc ataatgtgca gctgacctat tcctacgcca ctgttccggg cgagatcctg
     1621 acgggacacg atattgttac gcattcaaag atcctgaccg atccttacac gggaaagcaa
     1681 atggttctgc ccggtttggg ggtgactacc attccggtct ttttcacctt atttacctcc
     1741 atgttcatgc atgggggact gttgcacctg gccgggaata tgctattcct gttcgttttt
     1801 ggcgataatg tggaagatgc gatgggccac gccaaatatc ttgtattcta tttgctttgc
     1861 ggcatgctcg cggggttgag ccatgtgttc gctaccttct ttctgggaca gagccttttg
     1921 atccccagcc tgggcgcatc gggtgctata tcggctgtgc tggcagcata tgtgcggttg
     1981 tttccgggta agcgggtcga tctatggttc ttccttttca ttgttccggt gcccgccttc
     2041 gtggtcgtgg gcatctggtt catctttcag gtgatcgatg gtctggggat gctgggcggc
     2101 cagcagacag gtgatgtggc ctatgccgcg catatcggcg ggtttttatt cgggctgttg
     2161 ctgacgcgct tttttgcagg aggatacctg gcgcgaagag catatcgaag aagggccggg
     2221 caatggtaga gccgtcagcg taggcggccg ggacctttta tcttatcaac accacggtac
     2281 cttttttcgc aatggacctg ccggtgtaat ccgtgccttt cagcatccac agataggcat
     2341 tggcgccctg caattggccg ttgatcctgc catcccatcc ctgaccgatc tgcgacgtgc
     2401 tgaataccag ctgcccccac cggttataga catggaagaa gtcgagtgac gaaataccca
     2461 tacaaattgg cctgaacaca ttgttgctgc ctgccgccgg agtgaaagca ttgggaacaa
     2521 aaat
//
LOCUS       DATGKE010000408         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_209667, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000408 DATGKE010000000
VERSION     DATGKE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209667"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VKU83_07090"
     CDS             <1..685
                     /locus_tag="VKU83_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI93355.1"
                     /translation="AGVSDRDGDGVVDSKDECPDTAGPVALLGCPDRDGDGIADIHDK
                     CPTVPGSRNFQGCPAPDTDGDSVNDDEDKCPLVKGVKENFGCPPIKAELLRKMRNASD
                     RVFFVRAKARIEPVSLYELDRVVEILHSDTLLRLRIEGYTDSEGPDARELKLSQRRAK
                     AVYNYLVKQGIAPGRLDWVGYGKAHPLASNKTSMGMARNRRVEMVLTNYPKDKAASQV
                     GKAAPNVKQ"
     gene            complement(785..1300)
                     /locus_tag="VKU83_07095"
     CDS             complement(785..1300)
                     /locus_tag="VKU83_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLI93356.1"
                     /translation="MVTNKQSAGILLYRLATKAQGPAQPPSLEVFLVHPGGPFWKNKN
                     AGSWTIPKGEFDDAEPALEAAIREFREETGTTLKGHFHALTPIRQKGGKRVFAWAIAG
                     DIDPSQVVSNTFEIEWPRGSGKLQTFPEIDKGGWFSLPEAREHINSAQIAFLDELVAS
                     LPTISLAPSRE"
     gene            1554..>2279
                     /locus_tag="VKU83_07100"
     CDS             1554..>2279
                     /locus_tag="VKU83_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93357.1"
                     /translation="MHRRTTQRLLFMQLFFILLVGKARGQDSCAVVPPRLAIVGGTAC
                     NWAAFGFRILNPDSSETYSWYYDSTLLWANQPGNQDSGQYYSNIIAPATGHYSFHVVA
                     TSVCGDTASSNVLYNSGGITPIITSSTGYWAVCGNMPLLLYSNSETNNHWLINGQLID
                     SSGPTLLATAAATYRLVVTTPAGCNDTSAAQTIVVDAGPPAPVVTPGGTQTICTGSTL
                     TLNSSSGSGNQWFENDVAAPGETG"
BASE COUNT          489 a          593 c          704 g          493 t
ORIGIN      
        1 ggccggcgtc agtgaccgcg atggcgacgg tgtcgtcgac tccaaggacg aatgtccgga
       61 cacggccggc cccgttgctt tgctgggctg tcccgaccgc gatggcgatg ggattgccga
      121 cattcacgac aaatgtccga ccgtgcccgg atccaggaat ttccagggct gcccggcgcc
      181 ggatacggac ggcgacagcg tgaacgacga cgaagacaaa tgtccgcttg taaaaggggt
      241 gaaggaaaac tttggctgtc cgccgatcaa agcggagctt ctgagaaaga tgcggaacgc
      301 ttcagaccgg gtcttctttg tccgggccaa ggccaggatc gagcccgtga gcttgtatga
      361 gctggaccgg gtggtggaga tcctccattc ggacacgctg ctcagactgc ggatcgaagg
      421 ctacacggac agcgaaggac cggatgcccg ggaattgaag ctttcgcagc ggcgcgcaaa
      481 ggcagtgtac aattatctgg tcaagcaggg cattgctccc ggcaggctcg attgggtagg
      541 ctatggcaag gcccatccgc tggcgtccaa caaaacgtcc atgggtatgg ccaggaaccg
      601 gcgggtggag atggtgttga cgaattaccc gaaggataaa gcggcaagcc aggtgggaaa
      661 ggcggccccg aacgtaaaac aatagcgaac gggcattcgc acgctactaa aatagtttga
      721 aggtaaatag gtatatggca aaaacgacct cgtccggggt cgtttttcgt tagcggagcg
      781 cggtctattc gcgcgaaggt gcgaggctga tcgtgggaag gctcgcgacc agctcgtcga
      841 gaaaggcaat ctgggcggaa ttgatgtgct cgcgggcttc gggcaaggaa aaccagccgc
      901 ccttatcgat ctcggggaag gtttggagtt tgcccgagcc ccggggccac tcgatctcaa
      961 acgtattgct gacgacttgc gaggggtcga tatcaccggc gatggcccag gcaaagacgc
     1021 gcttgccgcc tttctggcgg atgggcgtta gggcatggaa atgtccttta agtgtggtcc
     1081 cggtttcttc ccggaattcg cgaatagccg cctccagtgc aggttcggcg tcgtcgaact
     1141 ctcctttagg gatggtccag ctgccggcat tcttgttctt ccagaacggg ccgccggggt
     1201 gaacgaggaa gacttcgagg gagggcggct gcgcaggacc ctgtgctttg gttgcgaggc
     1261 ggtaaaggag aataccggca gattgtttgt tggttaccat ctcagcctat aggcgcaaac
     1321 aattggccaa aaatgagtat tttgtacgaa gatgctaagg ttgcctgctg ctatacttgt
     1381 tttaagcact ttccaggctg cttcaggcca attgccgggt cggttttcgg ccgatttgtc
     1441 aggaggatgc ggcctggaaa taccgcttgg aaattcctgg taatttgcct acttttcgag
     1501 tcccggtgtc gctatgagaa acacctgtcc ccaggtgaag accgcctaaa ctcatgcatc
     1561 gccgaacaac acaacggctg ctgttcatgc agcttttttt tattctgctc gttggcaagg
     1621 cccgtggtca agattcgtgc gcggttgtgc cgccgcggct tgccattgtg ggcggaacgg
     1681 cctgtaactg ggcggcgttt ggattcagga tccttaaccc tgattcctcc gagacatatt
     1741 catggtatta cgacagtact ttgttatggg ccaatcagcc gggtaaccag gattccggtc
     1801 aatattacag caacattata gcgccggcaa cggggcacta ttcttttcac gtggtggcga
     1861 cttccgtttg cggagacacg gccagttcga atgttttgta taacagtggc ggcattacgc
     1921 ccatcattac cagcagtacg ggttactggg ctgtgtgcgg caatatgccg ctcctcttgt
     1981 attcaaatag tgagaccaac aatcattggc taatcaacgg tcagctgatc gattcctcgg
     2041 gaccgacgct actggcgaca gcggccgcta cctacaggct tgtcgtgacg actcctgcgg
     2101 gttgcaacga taccagtgcg gcccagacta tcgtcgtgga cgccggacct ccggcgcctg
     2161 tggtgacacc gggcggaaca caaaccatct gcactggcag caccctcacg ctaaactcgt
     2221 cgtcggggag cggcaaccaa tggtttgaaa atgacgtggc ggcgcccggt gaaacaggg
//
LOCUS       DATGKE010000409         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1093014, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000409 DATGKE010000000
VERSION     DATGKE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093014"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="VKU83_07105"
     CDS             <1..1968
                     /locus_tag="VKU83_07105"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI93358.1"
                     /translation="LKGKPGVKIMIDGKPTYLSAQDLANYLKNMPAANLEQIEIMSQP
                     PAKYDAAGNSGVINLITKKNRNNGFNASLTLSAIIARYFKSPNSLTFNWRHGNVNLYG
                     NYGYAWWDGFNDNHSNNSLRENAATPFDRYTLQYTYGRYSDRGHNFRAGMDYFVNKKT
                     TLGLSVNGTVDKEWFTSGSRTFFYDSLGNYVQNNIAKSRNNTPQTHLGFNGNFTRKLD
                     TKGSELSADADYIFYRTPGTLYSDNYLYNTDGNLSASPYLLNGDLPSHIDIYSFKGDY
                     KKVISKDITLEAGIKTSYVRTDNNAIYTLFDDSLKSWTADTAMSNHFIYKENINAAYI
                     NWTQTIRKFSFQLGLRAEQTNSSGDQTIKAVDFRKSYLQLFPTTYLNYKPDDNNTFGI
                     SYGRRIERPSYQSLNPFRFQLDRYTYEQGNPDLQPQFSNNVELSYNYKGELNVSANYT
                     MTNGIISQALITFKAAGDSNYTTYQTSQNLASQRNLGLSVNYSKQIVKDWTLNVFFNL
                     YNNRYRGVVDSTNIDVSFVSFNASFNTQYHFKKGWTSELSGFYYARDYVSGVLLADGR
                     GMFSIGGSKQIWHNKGALKLNLRDPLYLMSFSAHSDLNKGLTYSHSIWDNRRIILTLV
                     YRLGKTGNQVQRRNSGASDEQSRVGGGNGQQ"
     gene            complement(2003..>2183)
                     /locus_tag="VKU83_07110"
     CDS             complement(2003..>2183)
                     /locus_tag="VKU83_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93359.1"
                     /translation="NREPASLFGHFPTMVDEEFAMLSNMMRKDARKLLQQLNNEGYID
                     KVESQNGVLWKRKTA"
BASE COUNT          572 a          644 c          508 g          459 t
ORIGIN      
        1 ctcaaaggca aacccggcgt gaagatcatg atcgatggaa aacctaccta tctgagcgcg
       61 caggacctcg ccaactacct caagaatatg ccggccgcca atctggaaca gatcgagatc
      121 atgagccagc cccctgctaa atacgatgcc gcgggaaatt cgggtgtcat caatctcatc
      181 accaaaaaga accggaacaa cggtttcaac gcctcgctga ccctcagcgc gatcatcgcc
      241 cggtatttta aaagtcccaa tagcctgacc ttcaactggc gccatggcaa tgtcaatctc
      301 tatggcaact atggctatgc ctggtgggac gggtttaacg acaaccattc caataacagc
      361 ctccgcgaga atgctgcaac gccgttcgac cgatacaccc tgcagtacac gtatggccgc
      421 tattccgaca gaggtcataa cttccgggcc ggcatggatt atttcgtcaa taaaaaaacc
      481 acgctgggct tgtctgtcaa cggtacggtc gacaaagaat ggttcacctc cggttcccgg
      541 actttttttt acgacagcct gggcaactac gtgcaaaata acatcgcgaa gtcccgcaac
      601 aatacgccgc aaacccatct cgggttcaat ggcaatttca cccgcaagct ggatacgaaa
      661 ggaagcgagc tgtccgcaga tgccgattac atcttttatc gcacccccgg aacgctctat
      721 tcggataact atctttataa tacagacggc aacctgtccg cttcccccta cctgctgaat
      781 ggagacctgc cctcccatat cgacatctat agttttaaag gcgactacaa gaaagtgatc
      841 agtaaggata tcacgctgga ggccggcatc aaaaccagct atgtgcggac ggacaacaac
      901 gccatctata cccttttcga tgattcgctg aagagctgga ccgcggatac tgccatgagc
      961 aaccatttca tctacaagga gaatatcaat gccgcctata tcaactggac gcaaacgatc
     1021 cggaaatttt cgttccagct cgggctgcgc gccgagcaga ccaattccag cggcgatcag
     1081 acgatcaagg ccgtcgattt caggaagagc tatcttcagc tttttccgac cacgtatctc
     1141 aattacaaac cggacgacaa taacaccttc ggcatctcct atggcagaag gatcgaacgg
     1201 ccgtcctatc aaagcctcaa ccccttccgt ttccagttgg accggtatac gtacgaacag
     1261 ggcaatcccg atctccagcc gcagttcagc aataacgtcg agctcagcta taattacaag
     1321 ggggagctga atgtctccgc caattatacg atgaccaacg gcatcatcag ccaggcgctg
     1381 atcaccttta aggcggccgg cgactccaat tataccacct atcagacctc gcagaacctg
     1441 gcttcgcagc gaaacctcgg cctatcggtc aactacagca aacagatcgt aaaggactgg
     1501 accctgaatg tctttttcaa tctctataac aaccgctatc gcggtgtggt cgacagcacc
     1561 aacatcgatg taagcttcgt ttctttcaac gccagcttca atacgcaata ccatttcaaa
     1621 aaaggctgga cgagtgagct cagtggcttt tattacgcgc gggactatgt gagcggggtg
     1681 ctgctggccg acggccgcgg aatgttctcg ataggagggt caaaacagat ctggcacaac
     1741 aaaggggcac tcaagctcaa tctgcgcgat ccgctctacc tgatgagctt ctccgcgcac
     1801 agcgatctca ataaaggtct cacctactcg cattccatct gggacaaccg ccggatcatc
     1861 ctcacgctgg tctaccggct gggcaagacc ggcaaccagg tccagcgaag gaacagcggc
     1921 gccagcgacg agcaaagccg ggtaggcggc gggaatggcc agcagtagat cgaccttgcc
     1981 ccagcgcccg gcgtgcggca gatcacgccg ttttccgttt ccacaacacc ccattctgcg
     2041 attctacctt atcaatatag ccttcattgt tcaattgctg caaaagcttg cgggcatcct
     2101 tgcgcatcat attgctgagc atggcgaatt cctcgtcgac catcgtcggg aaatgcccga
     2161 acagactagc cggctcgcga ttg
//
LOCUS       DATGKE010000410         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1441521, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000410 DATGKE010000000
VERSION     DATGKE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441521"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VKU83_07115"
     CDS             complement(<1..253)
                     /locus_tag="VKU83_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLI93360.1"
                     /translation="MNRRTVIRNMTLSAGASLLGPGLRSFAAGITEPFAPTWDSLAKY
                     TVPQWFREAKFGMWAHWGPQCQPEAGDWYARLMYIEGSPQ"
     gene            complement(255..>2467)
                     /locus_tag="VKU83_07120"
     CDS             complement(255..>2467)
                     /locus_tag="VKU83_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLI93361.1"
                     /translation="SIKGGPDRQNPSKGPGGYFPTGIGWYRKSFTAPQSWKGKEVSIF
                     FEGVYMNAEVFINGHSLGIHPYGYTGFCFDLTPWLHWAGENTIAVRVDNSQQINCRWY
                     SGSGIYRHVRIAVKDPVHVDGGSVAVTTSDATKVKATVKLDVLLKNDGTSVADIILWA
                     KIVDRDQRIVGAKEMTIWLPAKSECHVTPSITVDKPLLWSVDTPYLYRLRLELTAGGK
                     HRDEYTSTFGIRTIAFSTVNGFQLNGESMKMYGGCLHHDNGCLGAAAFDRAEERRVKL
                     LKAAGFNAVRTSHNPPSIAFLDACDRLGLLVIDEAFDGWRQAKNPYDYARYFGDSWRR
                     DLASMVLRDRNHPSIVTWSVGNEILERKSPDAINTAKDLVSLVHRYDTTRPVTSAMTT
                     WDKEWEMFDPLFAVQDIAGYNYQLFRAASDHQRVPSRIIVQTESYPRDVFYNWNLIRQ
                     NSYLIGDFVWTAMDYLGESGIGKNNYPGEPDGQFWEADQFPWHGSYCGDIDLIGCRKP
                     VSHYRNILNGHKEKLYMAVREPQPDSGTIKETLWSVWPTWESWTWPGREGKDMAVEVY
                     SKYPTVRLYLNDSLIGEKKCSEGSEYKVTFTLPYSRGILKAVGISPDGREHPVELRTA
                     GPPAAIKPIRDRRQLTADREDLSFIQIELVDSYGTTVPNAANRLDFIIDGPATLAGVG
                     NADMSDTDSYAGSSRKAWHGRALLVVRSTHKAGKITVTVRSPGLPNGVITINSN"
BASE COUNT          510 a          687 c          692 g          578 t
ORIGIN      
        1 actgtggaga gccttcgata tacatgagcc gcgcatacca atcgccggcc tccggctgac
       61 attgcggccc ccaatgagcc cacataccga attttgcttc gcggaaccat tgggggacgg
      121 tgtactttgc cagcgagtcc caggtggggg caaagggttc cgttattccg gccgcgaaac
      181 tccgcaggcc aggccccagc agagaggcgc cggcagacag ggtcatattg cgaatgactg
      241 ttcttctatt catcctaatt tgaattaatg gtgatgacgc cattgggaag accaggcgat
      301 ctcacggtaa ccgtgatctt cccggcttta tgtgtgctcc tgacgaccag cagtgctctg
      361 ccatgccacg ctttgcgcga ggaaccggca tagctgtccg tgtccgacat atcggcattg
      421 ccgacgccgg ccagggtggc ggggccatcg atgataaaat ccaggcggtt agccgcattc
      481 ggaactgtag taccgtagct gtcgaccaat tcgatttgga taaaggatag atcctcccga
      541 tccgcagtca gctgcctgcg gtcccggatc ggcttgattg ccgccggcgg gccggcagtt
      601 cgtaattcga ccggatgttc gcggccgtcg gggctgatgc caacggcctt cagtatgccg
      661 cgggagtaag gcaaggtaaa ggttacctta tattcgctgc cttcggagca ttttttttca
      721 ccgatcaggc tgtcgttcaa atatagccgg acagtggggt atttcgagta cacctcgacc
      781 gccatatctt tcccttccct gcccggccag gtccagcttt cccaggtcgg ccaaacggac
      841 caaagagttt cttttatcgt tccggagtcg ggttgtggct cccgtacggc catgtagagt
      901 ttttctttgt gaccgttcaa gatattcctg tagtgcgaga ccggttttct gcatcctatg
      961 agatcgatat cgccgcaata cgaaccatgc caggggaatt gatcggcctc ccagaactgt
     1021 ccatccggct cgcccgggta attgttcttc cctatgccgg attcgccgag atagtccatc
     1081 gctgtccaga caaagtcgcc gatgagatag ctgttttgtc tgatgaggtt ccaattgtaa
     1141 aagacatccc gcggatagga ctctgtttgc acaatgatgc gggatggtac ccgctgatga
     1201 tcggaagccg cgcggaataa ctggtaattg taacccgcta tatcctggac ggcaaaaagt
     1261 ggatcaaaca tctcccactc cttatcccag gtcgtcatgg ctgaggttac gggtcgggtg
     1321 gtatcgtacc gatggaccag cgaaaccagg tccttcgccg tgttgatggc gtcgggggat
     1381 ttcctttcca gtatttcgtt accgacactc caggtgacga tcgaagggtg gttgcggtca
     1441 cgtagcacca tcgaggcaag gtcgcggcgc cacgaatcgc cgaaataccg cgcatagtca
     1501 taggggttct tcgcctggcg ccagccatcg aaggcttcgt cgatcaccaa cagccccagc
     1561 cggtcgcagg catccaggaa ggcgatcgac ggcggattgt gggaggtacg tacggcattg
     1621 aagccagccg ctttcagcag cttcaccctg cgctcttcgg cccggtcgaa ggcagcggcg
     1681 ccgagacaac cgttgtcatg atgcaagcag ccgccgtaca ttttcataga ctcgccgttt
     1741 agctggaatc cgttcaccgt cgaaaaggcg atcgtccgga tgccgaaggt gcttgtatat
     1801 tcgtcccggt gcttgccgcc tgcggtcaat tccaggcgga gccggtagag atagggggta
     1861 tctaccgacc aaagcagggg tttatcgacg gtgatcgatg gggtgacatg gcattcgctt
     1921 tttgccggca gccagatcgt catctctttt gcaccgacga tccgctggtc gcgatcgacg
     1981 atcttcgccc ataggatgat atccgccaca gaagttccat cattcttaag cagtacatcc
     2041 agcttaactg ttgctttgac tttggtggca tcggatgttg tgacagccac actcccgcca
     2101 tcgacgtgca ccgggtcttt tacagcaatg cgtacatgcc ggtagatgcc ggagccgctg
     2161 taccagcgac agttgatctg ttgcgagtta tcgacgcgta cggcgatggt gttttcgccg
     2221 gcccagtgca gccatggtgt gagatcgaaa caaaaaccgg tatatccgta ggggtggata
     2281 cccagggaat gaccgttgat gaacacttcg gcattcatat aaacaccctc gaaaaagatg
     2341 cttacctcct ttcccttcca ggattgcggc gctgtaaaac tcttgcggta ccagccgatt
     2401 cccgttggaa aataaccacc cggccctttg gaggggtttt gccggtccgg cccaccttta
     2461 atgctcc
//
LOCUS       DATGKE010000411         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_962291, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000411 DATGKE010000000
VERSION     DATGKE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962291"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="VKU83_07125"
     CDS             complement(<1..1892)
                     /locus_tag="VKU83_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLI93362.1"
                     /translation="MKSPFRKLVRISVVLLILVLLFNFFGYYINRIRSLENKELIEAI
                     NRSGHQQTLSQRITKEAILILNSPDQQASAAPLRDTLSALISQFQQNQALLQQQAGST
                     PPPVPQQIFQIKLLLSTAQSYLESMTAIGQEIAQADSMLLVMNKKLYTRNLLYNETKY
                     NALLNEINQHYSELVSETNGEVATVDTGKLISLIIAIVGLAILVLEPAFKKGEKNYKD
                     LQRARNDLLNEKKFLASILQSQTNYVIRINRTGHFTYANPAFLKTFGYTEDEIQHVLF
                     YTTIFPKDMARCQQVASECWNNPGRVSRLLIRKPIGRSREFIWTEWEFLALSDDDGQV
                     NEIQGIGSNVSEKLQMEKSKEEAIRTLSYAMTYAKMGSWNLDFISSELFLSKEFKALL
                     AMDEEDPDKIPLDNFLHLYVVPEDFNLVAEEFAKAIQHKENTGYETSFSCRIITKQGW
                     MRYLSVKGKVVDEQMEFGIAQDITFQKEAENALLNSEQKFRLLAENSEDIISVHATDG
                     TIWYLSPSVTTVLGYEVDDIIGRSIAQYVHPDDRHKFLPADQVPAFAGNGDNSIIVRY
                     RVLRKEGTYIWLESIIKPIVDEAELVKLICTSRNITDQKIAQEKLKKKDHLLHAVAQA
                     THSLLI"
     gene            complement(1997..>2659)
                     /locus_tag="VKU83_07130"
     CDS             complement(1997..>2659)
                     /locus_tag="VKU83_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93363.1"
                     /translation="HRDVLQALKAAGMDSLPGAGAEILIDRVRRLISKGKCGAQEWLD
                     IMHEAHKLYITTSATMMFGHVETLQERMEHLVKIRQVQAMKPADAKGFLAFIPWTFQD
                     VDTLLTRIRGVHNLTTPEEYIRMIAISRIMLPNVKNIQASWLTVGKQTAQLCLHAGAN
                     DFGSIMLEENVVSAAGAPHRFTARGIQTAIREAGFEPQLRNQQYEWRDIPVNIEEQII
                     NY"
BASE COUNT          567 a          676 c          728 g          688 t
ORIGIN      
        1 ttgatgagca gcgaatgcgt tgcctgggct accgcatgaa gcagatggtc ttttttcttg
       61 agcttctcct gagcgatctt ctggtcggtg atattccgcg acgtgcaaat gagcttgacg
      121 agctccgctt cgtcgacgat gggtttgatg atgctctcga gccagatata ggtcccttcc
      181 ttgcggagga cccggtaccg gacgatgatg ctattgtccc catttcccgc gaaggcaggg
      241 acctggtccg ccggcaggaa tttatggcgg tcgtccgggt gcacgtattg tgcaatggac
      301 cgcccaatga tatcgtcgac ctcatagccg aggacggtcg tcaccgaagg tgagagatac
      361 caaatggtgc catcggtcgc atgtacgctg atgatgtctt cggagttctc cgcgaggaga
      421 cggaactttt gctcgctgtt cagcaacgca ttctccgctt ctttctggaa ggtgatatcc
      481 tgggcgatac cgaactccat ctgctcgtct acgacctttc ccttaaccga aaggtagcgc
      541 atccagcctt gcttggtgat gatgcggcag gagaaggagg tctcatagcc ggtattttct
      601 ttatgttgga tggccttggc gaactcttcg gccaccagat tgaaatcttc gggcactacg
      661 tacagatgga ggaaattatc cagggggatc ttgtccgggt cttcctcgtc catagccagc
      721 agggccttga actccttgct aaggaacagc tcactgctga tgaagtccag gttccaggaa
      781 cccatcttgg cataggtcat ggcataggaa agcgttcgga tcgcctcttc tttgcttttc
      841 tccatctgga gcttttcgga gacgttggac cctatcccct ggatctcgtt gacctggccg
      901 tcgtcatcgg agagggcaag gaactcccac tcggtccaga tgaactcgcg gctccggccg
      961 atcggcttgc gaatgagcag acgagagacc ctgccggggt tgttccaaca ttcgctggct
     1021 acctgctgac aacgcgccat atccttggga aagatggttg tatagaaaag gacatgctgg
     1081 atctcatcct ccgtatagcc gaaagtcttc aaaaaagcgg gattggcgta ggtaaaatgt
     1141 ccggtgcggt tgatccggat cacatagttg gtctgagatt gcaggataga agccaagaat
     1201 ttcttttcgt tgagcagatc gttccttgcc cgctgcagat ccttatagtt cttttcacct
     1261 tttttgaaag cgggttccag tactaagatg gccaggccca caatggcgat gatcagggag
     1321 atcagtttcc cggtatccac cgtggccact tctccgttcg tttcgctgac cagttccgaa
     1381 taatgttgat tgatctcgtt taaaagcgcg ttatacttcg tttcgttata taataaattc
     1441 cgcgtatata actttttgtt catcaccaac agcatggaat ccgcctgggc gatctcctga
     1501 ccaatggccg tcatggattc aagataagat tgggcggtgg agagcagcag cttgatctgg
     1561 aagatctgtt gcgggaccgg gggaggcgtg gacccggcct gttgctgcag aagcgcttga
     1621 ttctgttgaa attgggaaat aagagccgaa agtgtatccc gcagcggggc cgccgaggcc
     1681 tgctgatcgg gactattcag gatcagaatg gcttccttgg taatgcgctg gcttagggtc
     1741 tgctggtgtc cggatcggtt gatggcttcg attaactcct tgttttccag ggatcttatg
     1801 cggttgatat aatatccaaa gaaattgaag agcagaacca ggatcaacag gactaccgaa
     1861 atgcgtacta gtttgcgaaa agggcttttc attgaattga ataatgctcg tctatggttc
     1921 tattaggaac gacaaatcag cattttaagt ttccggaact acaaaaaaac tggggtaaat
     1981 acctataagt tggtagctaa tagtttatga tttgttcttc tatgtttacg ggtatatccc
     2041 gccactcgta ttgctgattg cgcagctggg gttcgaaacc cgcctcgcgg atcgccgtct
     2101 ggatccccct ggcggtaaag cggtgcggcg ccccggccgc acttacgacg ttctcttcca
     2161 gcatgatact gccgaaatcg ttggcgcccg catggagaca gagctgtgcc gtctgcttgc
     2221 ccaccgtcag ccaggaggcc tggatattct ttacattcgg cagcataatg cggctgatgg
     2281 cgatcatccg gatgtattct tcgggtgtcg taagattgtg aactccccgg atacgggtca
     2341 gcagcgtgtc tacatcctgg aaggtccagg ggataaaggc caggaacccc ttggcatcgg
     2401 caggtttcat ggcctgaacc tgtctgatct tcaccaggtg ctccatcctt tcctgcaggg
     2461 tctccacatg cccgaacatc atcgtagcgg aggtagtgat atatagcttg tgggcttcat
     2521 gcatgatatc cagccattcc tgcgccccgc atttgccctt gctgatcagc cggcggaccc
     2581 tgtcgatcag gatctcggcg cccgcccccg gaagcgaatc catgccggcg gccttcaacg
     2641 cctgcagcac atcgcgatg
//
LOCUS       DATGKE010000412         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1031648, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000412 DATGKE010000000
VERSION     DATGKE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031648"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VKU83_07135"
     CDS             <1..1765
                     /locus_tag="VKU83_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93364.1"
                     /translation="GLVLLDKGVTVAQVERQFPGFVKRHFDLHPGQKANLSLQPLADI
                     HFNETYRDDYSRQAHLPTLYGLMGIAIFILLLAVINFVNLSTAQSLQRTKEVGIRKVL
                     GSRRKDLIFQFFGETFVLTILAVILSLLITAPAIRLLHDYMPPGFSFQPSLAVLLFLA
                     GITIITTLLAGWYPAKVISALLPVLSLKGQATRSLQPNRFLHRSLIVFQFTISLAFII
                     ATVVVARQLHYVINTDLGFDKDAIITINANGPVKDREVLAQQLREVPGIGVVSSNGGT
                     PQASFHLSQDFTCSGSENGTVTAAFIDADTDYLRLFGIRLLAGRNFWANDSAHEYLLN
                     ETMASQFGFPQPQSALGQTVTPGGQGSMRFGPGITGAKRAPGIVVGIVRDFHSRSMHQ
                     AIGPVILTYSRQVPEISIRLAPQARQPESVATVLAEVQALWNTTYPHDEFSYKFFDET
                     IANLYQQEQRLSGLMRLAMIIAIAISCMGLLGLATFAAEQRQKEISIRKVMGATVVRI
                     FRMLTLDFLWPVALAFIIAAPLAWYFLHGWLKGFAYRTTTPWWIFGLCGLSAAAIAML
                     TVGVQALRAAYRNPVEALRAE"
     gene            2138..>3379
                     /locus_tag="VKU83_07140"
     CDS             2138..>3379
                     /locus_tag="VKU83_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93365.1"
                     /translation="MLKNYFRVALRNLRRHKLFSIINMLGLAIGISASLVIFLVVQYE
                     YSFDRFEKDGDRIYRVVSDFTSNGGATGHTRGTPAPLAEAVRKKVAGIDQTVSFRYYN
                     VGKIAVPTQEKTKLAVFKSRDNVIFADDRYFDLLAYHWLAGSPAALAKPGKVILSATR
                     AKQYFPTTPYADLLGRTIVYDDSIPTQVAGIVQDLDQQGHTDFTFKEFISLATLLDNP
                     GLRNNFHWDDLGANSHQQLYVSLDKGTAPAAVHAALQRIADRQFGEAEKKNNFTVEYN
                     LQPLDDLHFNGDYGNFTSDVARKPTLYGLMLVAIFLLLLGSINFINLTTAQAVQRAKE
                     IGIRKTMGGSRRQLIFQFLSETFLITTLAMTLSLILTPLLLRLFADFIPTDLHFSLTL
                     PSLWVFLVLLVLAVSLLAGFYP"
BASE COUNT          706 a         1027 c          859 g          787 t
ORIGIN      
        1 gggccttgtg ctgctcgaca agggggtgac ggtcgcacag gtggagcggc agttccccgg
       61 ctttgtaaaa agacattttg atttgcatcc cggacagaag gccaaccttt cccttcaacc
      121 gctggccgat atccatttca acgagacgta ccgcgacgat tattcccgcc aggcgcacct
      181 gcccacccta tatgggctaa tggggatcgc catctttatt ctgctgctcg cggtgatcaa
      241 cttcgtcaat ctctccaccg cccaatcgtt gcaacggaca aaggaggttg gtatccgcaa
      301 agtactcggc agccggcgga aagacctcat ctttcaattt ttcggcgaaa ctttcgtgct
      361 caccatcctg gcggtgatcc tgtcactatt gatcacggcg ccggcgatcc gcctgctgca
      421 tgattatatg ccgcccggct ttagcttcca accctcgctc gccgtcctgc tcttcctggc
      481 tggcatcacg atcatcacca ccttgctggc cggctggtat cccgcgaagg tgatctccgc
      541 gctgttgccc gttctgagcc ttaagggtca ggccacccgc agcctgcaac ccaatcgctt
      601 tcttcaccgc agccttatcg tttttcaatt cactatttcg cttgcattta ttatcgccac
      661 cgtcgttgtt gcccggcaac tgcactatgt catcaacaca gacctcggat ttgacaaaga
      721 cgccattatc acgatcaacg caaacggtcc ggtcaaggac cgggaagtgc tcgcgcagca
      781 gcttcgtgaa gtccccggca tcggagtggt cagcagcaac gggggtacgc cgcaggcttc
      841 tttccacctc agtcaggact tcacctgttc cggatccgag aatggaacgg ttacggcggc
      901 cttcatagac gccgacaccg actatctccg gctatttggg atcaggctgc tcgcgggtcg
      961 gaatttctgg gcgaacgact cggcccatga atatcttctc aatgagacga tggccagcca
     1021 gttcggcttc ccgcagccgc agtcggcact tggacaaacc gtcacgccag gcgggcaggg
     1081 ctcgatgcgt ttcggcccgg gcattaccgg ggcaaaaaga gccccgggga tcgttgtcgg
     1141 catcgtccgg gatttccatt cgcgttcgat gcatcaggct atcgggccgg tcatcctcac
     1201 ctacagccgg caggtgcctg agatcagcat ccgccttgct ccccaagcac gccagccgga
     1261 atccgttgcc actgtgcttg ccgaagtgca agcgctgtgg aacacgacct acccgcacga
     1321 tgagttctcc tacaagttct tcgatgaaac catcgctaac ctttatcagc aggaacaacg
     1381 cctctcaggg ctgatgcggc tggccatgat catcgcaatc gcgatttcct gcatgggcct
     1441 gctgggtctt gcgacctttg cggccgaaca gcgtcaaaaa gagatcagta tccgcaaggt
     1501 gatgggcgcc actgtcgtgc gcatcttccg gatgctgacg ttggacttcc tctggcctgt
     1561 cgccctggcc tttattatcg ccgcgccgct ggcctggtat ttcctgcatg gatggctaaa
     1621 ggggttcgcc tatcgcacga cgacgccgtg gtggatcttc gggctctgcg gcctttccgc
     1681 ggccgccatt gccatgctga ctgtcggagt gcaggccctc agggccgcct atcggaatcc
     1741 tgtggaggcg ttgcgggcgg agtgatttgt cggaataaca ggttctatcg aatcacaaaa
     1801 cccgctacca gagcgggttt tggagaggtg agacaaaatc gtgacccgga ttggacactt
     1861 ttcaaaccac ttcctggcgg atttgagatt ggtcgaggaa ctaagtgcct gatactgcta
     1921 acgctgcatt tccattgttg tatcctattt ccggattttg taaaacattc caattattta
     1981 caaataatca acatgatggc cgtagaggat tcggccaccg actgtatcaa aatcggacac
     2041 tcgcgccgac tatgctggat acctatttga taggcaatta tttattagtt ggcaagctct
     2101 ttgtgccata ttggcacccc cctccaaaat caacaccatg ctaaaaaact atttccgggt
     2161 cgcgctgcgg aacctccgcc gccacaaact cttctccatc atcaacatgc tggggcttgc
     2221 catcgggatc agcgcctcgc tcgtcatttt tctggtcgtg caatatgagt atagctttga
     2281 caggtttgaa aaggacggcg accggatcta tcgggtggtc agcgacttca cctctaatgg
     2341 tggcgcgacc ggtcatactc gcgggacgcc ggcgcccctg gccgaagccg tcagaaagaa
     2401 ggtcgccggg atcgaccaaa cggtgagctt ccgttattac aacgtgggta aaatagccgt
     2461 acccacccaa gaaaaaacaa aactggccgt tttcaaatcg cgggataacg tcatcttcgc
     2521 ggatgaccgc tatttcgatc tcctggctta tcattggctg gccggttcac cagccgcact
     2581 ggccaaaccc ggtaaggtaa tcctcagcgc gacccgggcg aaacaatact tccccactac
     2641 gccctatgcc gatctcctcg gccgcaccat cgtctatgac gacagcatcc cgacacaggt
     2701 ggcggggatc gtgcaagacc tcgaccagca gggccatact gatttcactt ttaaagaatt
     2761 catttcgctg gctaccctcc tcgacaaccc cggtctgcgg aacaatttcc attgggacga
     2821 cctgggagca aacagccacc agcaactcta tgtcagcctc gacaagggca cggcgccggc
     2881 cgccgtccat gccgcgctgc agcggatcgc cgacaggcaa ttcggtgagg ccgaaaagaa
     2941 aaacaatttt accgtcgaat acaacttaca accgctcgac gatctccatt tcaacggcga
     3001 ctatggcaat tttacgagcg atgtagcgcg caaacccacg ctctatgggt tgatgctagt
     3061 cgctatcttc ctcctgctgc tcggcagcat caattttatc aacctcacca ccgcgcaggc
     3121 cgttcaacgg gcaaaggaga tcggcatccg caagacgatg ggcggatcgc gtcgccagct
     3181 catcttccaa ttcctcagtg agaccttcct gataacgacc ctggctatga ccctgtccct
     3241 catcctaacc ccgctcctgc tacggctttt tgccgatttt atccctacgg acttgcattt
     3301 ttctttgacg ctgccctcgc tatgggtctt cctggttttg ctcgtcctgg cggtaagtct
     3361 gctggcaggt ttttatccg
//
LOCUS       DATGKE010000413         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1143684, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000413 DATGKE010000000
VERSION     DATGKE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143684"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..2247
                     /locus_tag="VKU83_07145"
     CDS             25..2247
                     /locus_tag="VKU83_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HLI93366.1"
                     /translation="MRKLVTALFLFSSLAGASQTRTLTGFTERDARRQLDLETRFDGY
                     LRAADIDSGIRIMAARPHHVGSPGDRAVVDYISGKLKSWGYDVNVETFYVPFPTPRER
                     LLEMTAPTLFRASLAEKPLKEDATSGQTSEQLPTYNCWSPDGDVTGELVYVNYGIPED
                     YDYLQRLGISVKGKIVIAKYGRSWRGIKPKVAQEHGAIGCLIYSDPADDGYYAGDVYP
                     KGAFKNEWGVQRGSVLDMPIYPGDPLTPGVGATKDAKRLDRSEAPTLLKIPVLPISYH
                     DAEPLLRSMDGPVAPADWRGALPFTYHIGPGKTRVHLKLAFNWDMKELHDVIARLPGS
                     EWPDEWVIRGNHHDGWVNGAADPISGQAAMLEEARAVGELVKTGWRPKRTIIYCAWDG
                     EEPGLLGSTEWVEGHAAELQQKAVIYINSDDNGRGYLGVEGSHALEPFMNEVARDVID
                     PETHLSVAERMQVRDLVNAANRGLLKKDLFEKKSFTIGAMGSGSDYSPFIQHLGIPSF
                     NLGFGGEDPGGDYHSIYDSYDDYRRFKDPSFQYGVALAQTAGRTTLRMADANLLPFDF
                     RALYKTIDGYSKDLMAAVDQMRETTNLTNRLIRNHRFDEATDTAYHLMQPPVREEVPF
                     LDFSSLQNALDGLEKVTDRLADSLATSRLAETPSQAVNEALFKAEQQLLSDKGLPRRP
                     WYRHTIYAPGFYTGYGVKTMPGIREAIEQRNWKEAQEQIIIDAAVLRKFSDYLGAIAR
                     "
     gene            2320..4359
                     /gene="uvrB"
                     /locus_tag="VKU83_07150"
     CDS             2320..4359
                     /gene="uvrB"
                     /locus_tag="VKU83_07150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HLI93367.1"
                     /translation="MAFRLHAPYPPAGDQPEAIRQLTEGILEGERYQTLLGVTGSGKT
                     FTIANVIQNVQRPTLVLTHNKTLVAQLYGEFKQFFPENAVGYFVSYYDYYQPEAYMPV
                     SNTYIEKDLSINQELEKLRLQATTQLLSGRRDIIVVASVSCIYGIGNPAEFQNGIIRL
                     NRGQTVSRQGFLHSLVNSLYKRTEIEFSRGHFRVKGDTVDINLPYVDYGYRVSFFGDE
                     IDSIESFDIATGKRITQMEDAAIFPANLYLAPKDILQQVIYEIQDELAAQVAYFKSIG
                     RYIEAQRLSERVNYDVEMIRELGYCNGVENYSRFFDRRQPGTRPFCLLDYFPGDFLLV
                     LDESHQTVPQISGMYGGDRSRKLVLVDYGFRLPSALDNRPLNFHEFESMTGQTIFVSA
                     TPGDYELEKTGGVVVEQVVRPTGLLDPPIDVRPSVNQIDDLLDEIDKRVRKGDRVLVT
                     TLTKRMAEEMDKYLHRIDIKSKYIHSEVDTLERVEILRQLRLGEIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNEKSLTQTAGRAARNVDGLVIFYADTITESMQRTMDET
                     LRRREKQLAYNEAHGIVPQTIRKSKEQVFAQTSVLDIKGYNPADPYAVAPDEELVAVV
                     AEDQPVYETIPQVEKAIARTKKEMEKAARDLDFMEAARLRDEMFGLQKRLKEMKE"
     gene            4419..>7112
                     /gene="polA"
                     /locus_tag="VKU83_07155"
     CDS             4419..>7112
                     /gene="polA"
                     /locus_tag="VKU83_07155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLI93368.1"
                     /translation="MENTDKRVFLLDAMALIFRAYYALISSPRITSKGRNTNAQFGFT
                     NALIELINNQHPSHMAVCFDTEAPTERHTDFAAYKANRQEAPEDLVSALPDIKAIVKG
                     FNIPCMELDGYEADDVIGTLSKQAAASGYEVFMVTPDKDYGQLVTEKVRIYKPGYQGG
                     AAEILGPKEVCEKWNIKDVSQVIDILGLMGDAVDNIPGIRGVGEKTAAKLLGEWGSLE
                     NVVANAENIKGALGEKVRAGKELALMSKKLATIITDVPVEFHEENFRLKEWNRDALNE
                     IFTALEFKTIGKRILGEGFGGGGYVPPEGVQTDLFGNTVTQAAAKKKAAAYAGAPSAK
                     NSGAEEAAPAGGLAGERLERLEEFSTRLESVVERGAEAGVTGGESTGGESTGVEGEAA
                     TTIPPGARNIRNTPHTYMAVVGDQAIADLVQRLRNQNEICFDTETTGIDPNDAELVGL
                     SFCYRPGEAWYVPCPADQAEAKKILAAFQPLWDSESITWVGQNLKYDWLMLKWYGIEP
                     RGKIFDTMLAHYVIEPEGKRGMDLLSTQFLGYEPVHIEELIGKKGKGQGNMRDVELDK
                     IKDYAAEDADITLQLKHVFFPQLKNKSVEKVFYEVENPLVRVLTEMEFEGIRVDMEFL
                     REYSKELEREARQAEEKVYQQAGERFNLSSPKQLGEVLFEKMQLDPKAKKTKTGQYAT
                     GEDVLLKLAVRHQIVDDILAFRELTKLRSTYVDALPLMINRKTGRVHTSYAQAVAVTG
                     RLSSNNPNLQNIPIRTERGREIRKAFIPRNDEYVLVSADYSQIELRIVAAISRDENMC
                     AAFRDGKDIHTATAAKVFGVDEKAVTKEMRYKAKSVNFGIIYGQGAFGLADNLGISRT
                     EAKQIIDNYKQQFSGIQRYMDSTNNFAREHGYVETLIGRKRW"
BASE COUNT         1675 a         1901 c         2197 g         1339 t
ORIGIN      
        1 ggcgcaggca taaataaatg ctttatgaga aaacttgtaa ccgctttgtt tcttttttcc
       61 tcgctggcgg gcgcatccca gaccaggacc ctgaccggat tcacggagcg ggatgcccgg
      121 cggcagctcg acctggaaac caggtttgac ggctatttgc gggctgccga tatcgattcg
      181 ggtatccgga tcatggctgc caggccgcac catgtgggat cgcccgggga ccgggcggtg
      241 gtcgactaca tttctggcaa gctgaaaagc tggggctatg acgtgaacgt ggaaaccttt
      301 tacgtaccct tccccacgcc cagggaacgg ttgttggaaa tgaccgcacc cactctgttc
      361 cgggcttcgc tggccgaaaa acctttgaag gaggatgcta cctccggtca gacctccgaa
      421 cagctcccta cctataattg ctggtcgcct gacggggacg tcaccgggga gctcgtttat
      481 gtgaactacg gcatcccgga agactacgac tacctgcagc ggctgggaat ctccgtcaaa
      541 ggcaaaatag tgattgccaa atacggaagg tcatggcggg ggatcaaacc caaggtagcg
      601 caggaacacg gcgccatcgg ctgcctgatc tattcggatc cggcggacga cggttactat
      661 gctggtgatg tatatcccaa gggagctttt aagaacgagt ggggggtcca gcgagggtcg
      721 gtcctcgata tgccgatcta tcccggcgac ccccttaccc ccggggtcgg cgcaacaaag
      781 gacgccaaac ggctggacag atccgaggct ccgacccttc taaagatccc cgtgctgccc
      841 atctcctacc atgacgccga acccctgctt cggtcaatgg acgggccggt cgctcccgcg
      901 gactggcgcg gcgcccttcc tttcacgtat cacatcggac ccggaaagac acgggtccat
      961 ctcaagctgg cgttcaattg ggatatgaag gaactgcatg acgtcattgc ccgccttccg
     1021 ggcagcgaat ggccggatga atgggtcatt cgcgggaacc accacgatgg ctgggtgaac
     1081 ggggccgccg accccatcag cggccaggca gccatgttgg aggaagcccg ggcggtcggg
     1141 gagctcgtca agaccggctg gaggccgaag cgtaccatta tctattgcgc atgggacggc
     1201 gaggagcccg ggttgctcgg atctacggaa tgggtagagg gccatgcggc ggagcttcag
     1261 cagaaagcgg tgatatacat caattccgac gacaatggcc ggggttacct cggcgtggaa
     1321 ggatcacatg cgctggagcc cttcatgaat gaggtcgccc gggacgtcat cgaccccgag
     1381 acacacctgt ccgtcgcgga gcggatgcag gtccgcgatc tggttaatgc ggccaaccgg
     1441 ggccttctca aaaaagacct tttcgaaaag aagtcgttta cgatcggggc gatggggtcc
     1501 ggatcggact attcgccgtt tatccagcac cttgggattc cttctttcaa cctcggattc
     1561 ggcggcgaag accccggcgg cgactatcac tccatctatg attcgtatga tgattaccgg
     1621 cgatttaagg acccgagttt ccagtatggc gtggcgcttg cgcagacagc cggccggacc
     1681 accctgcgaa tggccgatgc caatctgctg cccttcgatt tccgggcgct ttataagact
     1741 atcgacggtt atagcaagga tctgatggcc gcggtcgacc agatgcgcga gacgacgaac
     1801 cttaccaacc ggctcatcag gaaccaccgg tttgacgagg cgacggatac cgcctaccac
     1861 ctgatgcagc ccccggtcag ggaggaggtg ccttttcttg acttctccag cctgcaaaac
     1921 gcattggacg gcctcgaaaa ggtaacggac cggctggcag acagcctcgc cacctccagg
     1981 ttggcggaga ctccgtccca agccgtcaat gaagcgcttt tcaaagccga acagcaattg
     2041 ttgtcggaca aggggcttcc gcggcggcca tggtaccggc acacaatata tgctcccggt
     2101 ttctataccg gctacggggt gaaaacgatg cccgggatcc gggaagcgat cgaacaaaga
     2161 aactggaaag aggcgcagga acagatcatt attgacgccg ccgtgctgag gaaattttcc
     2221 gactacctgg gggcgatcgc cagataggga agcctgggtg ccgggaggag cgctttcggg
     2281 ccaaaaaacc gtaattttac cggttaccaa atctttttca tggcattcag gctccacgca
     2341 ccttacccgc ccgccggaga tcagcccgag gcgatccggc aactgacgga aggaattctg
     2401 gaaggcgagc gataccagac tttactgggg gtcaccggtt ccggaaagac cttcaccatc
     2461 gcgaacgtca tccagaatgt tcaacgccct accctcgtcc tcacccacaa caagaccctc
     2521 gtggcgcaat tatacgggga attcaaacaa ttctttccgg aaaatgcggt cggctacttc
     2581 gtttcttatt acgattatta ccagccggag gcttatatgc cggtgagcaa tacctatatc
     2641 gaaaaggacc tgtcgatcaa ccaggaactg gagaagctcc gcctgcaggc gaccacgcag
     2701 ttgttgagcg ggcgcaggga tatcatcgtc gtcgcgtcgg taagttgcat ctacggcatc
     2761 ggcaatccgg cggagttcca gaacgggatc atccggctca accgcggcca gacggtatcg
     2821 cgtcagggat tcctgcactc gctcgtcaac tccctgtata agcgcaccga gatcgaattt
     2881 tcgcggggcc acttccgggt caaaggcgac acggtcgaca tcaatcttcc ctatgtcgac
     2941 tatgggtaca gggttagttt tttcggtgac gaaattgact cgattgaaag ctttgacatc
     3001 gctaccggca agcggatcac ccagatggag gatgccgcga tttttcccgc caatctctac
     3061 ctggccccca aggatatcct gcagcaggtg atctatgaga tccaggacga actggcggcc
     3121 caggtcgcct atttcaagag catcggcaga tatatcgagg cccagcggct gagcgagcgg
     3181 gtcaactatg atgtggagat gatccgggaa ttgggttatt gcaatggggt cgaaaactac
     3241 tcccgctttt ttgaccggcg gcagcccggg acgagacctt tctgcctgct ggattatttt
     3301 cccggcgact ttctcctcgt gctggatgaa agccaccaga cggtaccgca gatcagcggg
     3361 atgtacggcg gtgaccggag ccggaaactg gtgctcgtgg actatggctt caggctcccc
     3421 agcgcgctcg acaaccggcc gctgaatttc cacgagttcg aatcgatgac cgggcagacc
     3481 atctttgtct ctgccacgcc cggcgactac gagttggaga agaccggcgg cgtcgtcgtg
     3541 gaacaagtgg tgcggcctac cgggctcctg gatccgccga tcgacgtgcg gcccagtgta
     3601 aaccagatcg acgacctgct ggacgagatc gacaagcggg ttcgaaaggg agaccgggtg
     3661 ctggtgacga cgcttaccaa acgaatggcg gaagaaatgg acaaatacct tcaccggatc
     3721 gatatcaaat ccaagtatat ccatagcgaa gtcgacacgc tcgagcgggt ggagatcctt
     3781 cgccagttgc ggcttggaga gatcgacgtg ctggtaggcg tgaacctctt gcgggagggg
     3841 ctggacctgc cggaagtatc gctcgtggcg atcctcgatg cggataagga aggatttctg
     3901 cggaatgaga aatcgctcac gcaaacggcc gggcgcgccg cgcggaacgt cgacggtctc
     3961 gtcatctttt atgccgatac catcaccgaa agcatgcagc ggacgatgga tgagacgttg
     4021 cggcggcggg agaagcagct ggcctacaac gaggcgcacg ggatcgtgcc gcagaccata
     4081 cgcaagtcga aggaacaggt ttttgcacag acctccgttc tggacatcaa gggatacaat
     4141 ccggcggatc cgtatgccgt cgcgcccgat gaggaattgg tggccgtcgt ggcggaggac
     4201 cagccggtct acgagaccat cccgcaggtg gaaaaggcga ttgcccggac gaaaaaggag
     4261 atggagaagg cggcgcgcga tctggacttt atggaggcgg caaggttgag ggatgagatg
     4321 ttcggtctgc agaaaaggct gaaggaaatg aaagaataaa tccgccgggc cagtacaatc
     4381 ccggcaaggc ttcgcgggaa tttggctaat ttcgccgcat ggaaaatacc gacaaaagag
     4441 tgttcctcct cgatgcgatg gccctcatct tcagggcata ttacgcgctg atcagcagcc
     4501 cgcgcatcac cagcaagggt cgaaacacca acgcgcagtt tggtttcacg aatgcgctga
     4561 tcgagctcat caataaccaa catccttcgc atatggcggt atgtttcgat accgaggcgc
     4621 ccacggagcg gcacacggat tttgcggcct acaaggccaa ccgccaggag gcgccggagg
     4681 acctggtatc ggcgctgccg gacataaagg cgatcgtcaa aggattcaat attccctgca
     4741 tggaactgga cggttacgag gcggacgacg tcatcgggac cttaagcaag caggcggcgg
     4801 cgagcggtta tgaagtcttc atggtgaccc ccgacaagga ttacggccag ctcgtgacag
     4861 aaaaagtgag gatctacaaa ccagggtacc agggtggcgc ggcggagatc cttggaccga
     4921 aagaggtttg tgaaaaatgg aatatcaagg acgtgtcgca ggtcatcgat attttgggac
     4981 tgatggggga cgcggtagat aatatccccg gtatccgggg cgtgggagaa aagaccgcgg
     5041 ccaagttgct gggtgagtgg ggcagtctgg agaacgtcgt agcgaatgcc gaaaatatca
     5101 agggcgcgct gggagaaaag gtgagggccg gaaaggagct tgccctgatg agcaaaaagc
     5161 tcgcgacgat catcaccgac gttccggtag agttccatga agaaaatttc cggctgaagg
     5221 aatggaaccg cgatgccctg aatgagattt ttacggcgtt ggagttcaag accattggga
     5281 agaggatcct cggcgaaggc tttggcgggg gtggctatgt cccgccggaa ggcgttcaaa
     5341 cggatctttt cggaaatacg gtgactcaag cggcggcaaa aaagaaagcg gccgcctacg
     5401 ccggcgcgcc gtcagccaaa aacagcggcg cggaagaagc cgctccggcg ggcggcctgg
     5461 cgggcgaaag actggaacgc ctcgaggagt tcagcacacg gctcgagtcc gtggtggaaa
     5521 ggggcgccga agccggggtg acgggcggcg agtcgacggg cggtgagtcg accggggtcg
     5581 agggcgaagc ggcgacgaca atcccacccg gagcgagaaa catccgcaac actccccaca
     5641 cctatatggc agtcgtgggt gaccaggcga tcgccgatct ggtgcaacgg ctaaggaacc
     5701 aaaacgagat ctgtttcgac accgaaacca cgggaatcga tccgaacgat gcggagctcg
     5761 tcggtttgag cttctgctat cggcccggcg aagcgtggta cgtaccctgt cccgcggatc
     5821 aggcggaagc aaagaagatc ctcgcggcct tccagccgct ctgggattcc gaatccatca
     5881 cctgggtcgg acaaaacctg aagtatgact ggctgatgct gaaatggtat gggatcgagc
     5941 cgcgcggcaa gatcttcgat accatgctcg cccattatgt gatcgagccg gaggggaagc
     6001 gcgggatgga tttgctgagt acgcaattcc tcggctatga gccggtccat atcgaagagc
     6061 tgatcgggaa aaaaggaaag gggcagggga acatgcggga cgtggagctt gacaagataa
     6121 aggactacgc ggcagaggat gcggacatta ccctgcaact aaagcatgtt ttttttccgc
     6181 agttgaagaa taaatcggtc gagaaggttt tttacgaggt agagaatccg ttggtccgcg
     6241 tactgaccga aatggaattc gaggggataa gggtggatat ggaatttctc cgcgagtatt
     6301 ccaaagaatt ggagcgggag gcgcgtcagg ccgaagagaa agtgtatcag caggcaggcg
     6361 agcggttcaa cctgtcttcg ccgaaacaac tgggggaggt gctttttgaa aaaatgcaac
     6421 tggaccctaa ggccaaaaaa acgaaaaccg gtcaatacgc caccggcgag gatgtgctgt
     6481 taaagctggc cgtgcggcat cagatcgtcg atgatatcct cgccttccgg gagctcacca
     6541 agctgcgttc aacctatgtg gacgccttgc cgctcatgat caaccgcaaa acggggcggg
     6601 tccataccag ctatgcgcag gcggtcgcag tgacaggtag gctgagctcc aacaacccca
     6661 acttgcagaa tatcccgatc cggacggagc gcggccgcga gatccggaag gcgttcatac
     6721 ccaggaacga tgagtacgtg ctggtttccg ccgattattc gcagatcgag ctgcgcatcg
     6781 tagcggcgat cagcagggat gagaatatgt gcgccgcatt ccgggacggc aaggatatcc
     6841 atacggcgac ggcggcgaag gtattcggcg ttgacgaaaa ggcggtgacg aaggagatgc
     6901 gctataaagc caagagcgtg aatttcggga tcatttacgg ccagggggct tttgggctgg
     6961 ccgacaacct gggcatcagc cgcacggagg caaaacagat catcgataat tacaagcaac
     7021 agttctcggg gatccaacgc tatatggatt ccaccaacaa ttttgcccgc gagcacggct
     7081 atgtggagac cctgatcgga cggaagcgct gg
//
LOCUS       DATGKE010000414         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_353382, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000414 DATGKE010000000
VERSION     DATGKE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353382"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VKU83_07160"
     CDS             <1..508
                     /locus_tag="VKU83_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93369.1"
                     /translation="PANSVLLDGSGSTDPAGETLAYQWSQVSGPAAAALGSAGAVTTT
                     AGQLQAGLYTFQLKVTNTSGLSAVATVQVRVIDNQRSVDSGNAAVLIYPNPVAAMLTV
                     KCTDPSTSGQVLMQVVTMNGVMVMENEAEVTGAESINFNLSKLARGVYGLRVIVGSRQ
                     TYQLIVKQ"
     gene            1053..>2858
                     /locus_tag="VKU83_07165"
     CDS             1053..>2858
                     /locus_tag="VKU83_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93370.1"
                     /translation="MKSVSSPLHNGIGVDMNGNVWFWGANDGGMRGDGTVGGATSFVP
                     VQVARDSSGNPFNNVNQVSCFYNEVLSPTGAICTGALVCKNDGTVWIWGNTIGGMRGN
                     GQHGQENYKPVQVNIPGNPHIVKVLAGEICMALDINGNVYTWGGNGRTDLLGTNNPDY
                     THVHQVILPKPAKDIAGADMFAYALCADGTLYGWGIYGAYLGDGTGTYLASNVFTPLP
                     VDLTAGLHLPHPISQIMCNSVSTHVILTDSTLWGWGDNTQGCIGNGVMENWATYHTPY
                     AWDWGAAEILQHQPVQIAPLVHNFVAIFAGTSACFYYYAETATGQLYSWGRNKSIVLG
                     NGITSGTDEQVSYPDAWNQPTITAVNPFALTKTYNSPCPYCLANHGTYPCSEYAIPAD
                     AAPVAVPGANKTISLPASTAILDGSGSHDPDGEVVYYKWRQVSGPSANTVLDSTYAQT
                     RLRGLVAGTYVYSLTVTDNGWATNTANVTVTVTSTGNVAPTVSGGSNVTITLPATTAS
                     LTGTATGNNGATIKTVSWKQTSGPATATIASPGSLSTGLSGLTVAGTYSFSLTATDNN
                     NLTGTASVSVVVNAATTATPPTVSAGKGPDITLPTS"
BASE COUNT          629 a          783 c          833 g          613 t
ORIGIN      
        1 gcctgccaat tcggtgctgc tggatggaag cggctcgaca gacccggctg gagagacatt
       61 ggcctatcaa tggtcccagg tcagcgggcc agccgcagcg gcattggggt ccgcgggcgc
      121 ggtgacgacc acggcgggcc agctgcaggc cggattatat acgttccaac tgaaggtgac
      181 gaatacctcg gggttgagtg cggtcgcgac agtgcaggtc cgcgtgatcg acaaccagcg
      241 gagcgtggat tcgggcaacg cggcagtact gatatatcct aaccccgtgg cagccatgct
      301 gaccgtgaaa tgtaccgatc cctcaaccag cggccaggtc ctgatgcagg ttgtcacgat
      361 gaacggcgtt atggtgatgg aaaacgaagc cgaggtgacc ggcgccgaat caataaattt
      421 caatctttcg aagttggcga gaggagtcta tggattgcgc gtgatcgttg gatcgcgtca
      481 aacctatcag ctgatcgtca aacaataaaa aatatttttc cggcgcgaaa agggcgatct
      541 gtgatggatc gctctttttt tatatgtata ggcacacacg gcaataaaac attgtggacc
      601 gacacgccaa tcccgcgatc gccgccggcg acaatcacct gcgctcccag gcggcaaacc
      661 attcatccgg tacgcgtttt caggcgttcg tggcttattt ggtacacatt ttgtttactg
      721 gcaacggtaa attccggggt ccttcttgct accttagaga acatgaaaaa catgatttag
      781 cgtccggatc tatttttcat tccgatatct atttagacat gcgaaactcg ctgctgttgc
      841 tgatttcatg cgttggtctt tgtttccagg catcctcaca atcaaccccg cccatcgtcg
      901 tggctacggg ggaataccag gctttttttt atcacacttc agacagaatt ttatattcca
      961 ttggcggggc tgttggtacc cagggaattg gagctaatcc aacgcaacct ctcgggactg
     1021 caataaaggt gcagtttcct gccggcgttc aaatgaagtc ggtatcgtca ccgctgcaca
     1081 atggaattgg cgtggacatg aacggaaatg tttggttctg gggagcaaat gacgggggga
     1141 tgcgcggcga cggaacggtc ggtggtgcaa cctcttttgt cccggtacag gttgccaggg
     1201 acagttccgg caatccattt aataatgtaa accaggtatc ctgcttttat aatgaagttt
     1261 tatcgcctac cggagccatt tgcacgggcg cgctggtatg caagaacgat ggcaccgtct
     1321 ggatctgggg taacacgatc ggcgggatgc gcgggaatgg tcagcacggc caggagaact
     1381 acaagccggt gcaggtaaat attcccggca acccgcatat cgtcaaggta ttggccggcg
     1441 agatctgcat ggctctcgac attaatggta atgtatacac ctggggcggg aatggccgga
     1501 cggatctgct tgggaccaac aatccggact acacccatgt ccaccaggtg atccttccga
     1561 agccggcgaa agatatcgcg ggcgcggata tgtttgccta cgcgctgtgc gcggacggca
     1621 ccttgtatgg ctgggggatc tatggcgcct acctgggcga cggaacagga acctatctgg
     1681 cgagcaatgt cttcacgcct ttgccggtgg acctaactgc cggtctgcac ctcccgcatc
     1741 cgatctcgca gatcatgtgt aattcggtat ccacgcatgt tatcctgaca gacagtacgt
     1801 tgtgggggtg gggcgataat acgcagggct gcattggaaa cggcgtaatg gaaaattggg
     1861 cgacctatca tacgccttat gcgtgggact ggggcgcagc cgagatcctg cagcatcagc
     1921 ctgtccagat tgcgccgctg gtgcacaatt tcgttgccat ctttgccggt acctcggcct
     1981 gtttttatta ctatgccgaa acggcgaccg ggcaactgta ttcctggggg cgcaacaagt
     2041 ccatcgttct gggaaacggc atcaccagcg ggacggacga gcaagtatct tatcctgacg
     2101 catggaacca gccgacgatc acggctgtca atcccttcgc gctgacaaag acctataatt
     2161 cgccttgtcc ctactgcctg gcaaaccacg gcacttaccc gtgcagcgag tacgcgatcc
     2221 ccgcggacgc agcgcctgta gctgtcccgg gcgccaacaa aacgatcagc cttccggctt
     2281 ccacggcgat cctcgacggg tccggttccc atgacccgga cggcgaggtg gtatattata
     2341 aatggcggca ggtttccgga ccgtctgcca ataccgtttt ggattccacc tacgcccaaa
     2401 ccaggttgcg cggcctcgtg gcgggaactt atgtatatag tctgacggtc acagacaacg
     2461 gttgggcgac caatacggca aatgtcacgg tgacggttac atcgaccggc aatgttgcgc
     2521 cgacggtcag cgggggaagc aatgtgacga tcacgctgcc tgccactacc gccagcctga
     2581 ccggtaccgc gaccggtaac aacggcgcga ccatcaagac ggtcagttgg aagcagacat
     2641 cgggaccggc gaccgcgacg attgcgtcgc cgggcagcct gagcacgggc ctctcgggcc
     2701 tgaccgtagc cggcacctac agcttttcgc tgaccgcgac cgacaataat aatctgacgg
     2761 ggaccgcgtc ggtttccgta gtggtgaatg cggccacgac ggcgacgccg ccgacggtga
     2821 gtgcgggcaa gggccccgac atcacgctgc cgaccagt
//
LOCUS       DATGKE010000415         4706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_123017, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000415 DATGKE010000000
VERSION     DATGKE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123017"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..2498)
                     /locus_tag="VKU83_07170"
     CDS             complement(420..2498)
                     /locus_tag="VKU83_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLI93371.1"
                     /translation="MDNPPTINRRKFLKLTGGGLVVAFVFNDFLSFAGGRPGHGSPTV
                     PTTEVGAWIHIGQDGSISVYTGKVEFGQNIRTSLSQIVAEELKVPVSSIKMIMGDTDL
                     VPYDAGTFGSRTTPQMGTQLRRAAATAREALIEMAAKSWDAQPAALHAENGMIVNTAA
                     NVRLSYGDLTKGQDILRTISDDVPVVAAKDWKISGKSVPKVDGSAYISGKHAYTSDMH
                     VPGMLYGKVLRAPSYNAKLVSADISKAKALQGVSVVRDGDFIGVAATDLVTAEKALML
                     INANWQEEAGQPSNADIFDYLVKNASGERDADTNGDVAAGLAQADFRHSQTYYIHYIA
                     HTPLEPRAALAEWRDGKLTVWTGSQRPFGVQEELINTFRLDKDKARVIVPDTGSGYGG
                     KHSGEAAIEAARLAKAVGKPVKLVWTREEEFTWAYFRPGGVIDVTAGVKKDGTITSWK
                     FINYNSGSPGMDSQYKAVNRHIAHMPSKTPLRQGSYRALAATGNVFARECHMTDLARL
                     IKMDALEFRLRNLEDDRFKAVLQAAAKAFGWGAAASAGHGFGIAGAFEKGGHVATCAE
                     VMVTDEKEVKVLRVTQAFECGAIVNPHHLENQITGAIIMGLGGALFEKVEFASGKILN
                     SSLSDYRVPRFSDIPKIEVVLVNRKDLPSAGAGEAGIVGIAPAIRNAILDATGTGLTT
                     MPMIPNGTVT"
     gene            complement(2509..2958)
                     /locus_tag="VKU83_07175"
     CDS             complement(2509..2958)
                     /locus_tag="VKU83_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLI93372.1"
                     /translation="MVLNVNGVRHEITADPDVALLTVLRNDLQLTGSKYGCGVGACGA
                     CTVLVNGKPTHACITPINALAGKDITTIEGLEKDGKLHKVQEAFLTTDVFQCAYCASG
                     MLMSAVALLNSNPAPSEDAIVKAMNGNICRCGTYPLIVRAIKLASGS"
     gene            complement(3071..4189)
                     /locus_tag="VKU83_07180"
     CDS             complement(3071..4189)
                     /locus_tag="VKU83_07180"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93373.1"
                     /translation="MAELSLQHISKVYAAEGRGQDPKKAIDDVSFTVRDKEFMVIVGP
                     SGCGKSTLLRMMAGLEDISAGNLSIDGIRVNEAGPGERDIAMVFQNYALYPHMTVYDN
                     MAFGLRLRHYSKTEIRERVMQAAGLLDISAELNRKPRTLSGGQRQRVAIGRAIVRRPK
                     IFLFDEPLSNLDAKLRSQMRIELQKLHREINATMVYVTHDQVEAMTLGNRIAVLQKGK
                     LMQLDTPLNIYNKPENKFVAGFIGSPEMNFIRGVLDKSSGQYQLRVSADRSILLGDSV
                     PASLQAKTGEVMEIGIRPEHWDIVEPGRPSAVKAKAVAFENMGNEQIIYCSLDRQPII
                     VKRFAQDTVEIGQDFYLRPQKNKIVYIDSLTMRVLNDR"
     gene            complement(4176..>4706)
                     /locus_tag="VKU83_07185"
     CDS             complement(4176..>4706)
                     /locus_tag="VKU83_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93374.1"
                     /translation="EGKANNLYYALTRPMGECYFASPHQSAYHPGAAIHLALSPDLLY
                     WKPSNEPFLRARKSSAANLKVGGGTPPLRSRDGWLMLYHGVENNGEIGCYRTFWAILD
                     GDDPQTILRLEDQEPLLEASPELTAPISHQMYLHDVVFSTGIVAHKDQYIIASGESDL
                     ACRITVIEKNFFYGGA"
BASE COUNT          975 a         1301 c         1280 g         1150 t
ORIGIN      
        1 attttcccgc tgacgtgggc gtgccggata agacgccatc ggcggacaat gtcatgccgg
       61 aaggcaactg gccggaagaa atggaaaacg aataaggcgg cgccccgccg gtaaccttca
      121 gggtctgctt attatagacg gatccgtaaa ggccgtcagg cacagctgat ggcgttatga
      181 caattgttgg ttgcgcgaag agcgcggtcc caatgccggt caaaagcacg gtgaacgcaa
      241 aggtcttccc tttcataaaa gcgaaacgat ttgactacgg gagagatggg aatgatgctg
      301 ttagaaggat gttcgatttc agcggcgaag taaagatacg ggtttttaac gttcttttaa
      361 ctggttttcg ccggaactac gatatacgta tcgcacgatg ctacatttca tttttcacgt
      421 caggtcaccg tcccgttggg gatcatcggc atggtggtca gcccggtacc tgtagcatcg
      481 aggatggcat tccggatggc gggcgcaatg cccacgatac cggcttcgcc ggcaccggcc
      541 gagggaaggt ctttacggtt gacgagtact acttctattt tcgggatgtc gctaaagcgg
      601 gggacgcgat agtccgagag gcttgaattg aggatttttc cggaagcgaa ttcaaccttc
      661 tcgaagagcg cgccgccaag tcccatgata atggctcccg ttatctggtt ctcgagatga
      721 tgcggattga caatggcgcc acattcgaaa gcctgagtga cgcggaggac ctttacttct
      781 ttttcgtcag tcaccatcac ttcagcgcag gtcgcgacat gacctccctt ttcgaaggcg
      841 ccggcaatgc caaagccgtg accggcgctt gcagctgcac cccagccgaa ggcctttgcc
      901 gccgcctgaa gaactgcctt aaaccggtcg tcttcgaggt tcctcaggcg gaactccagg
      961 gcgtccatct tgatcaatct ggcgagatcg gtcatatggc actcgcgggc aaatacattt
     1021 ccggtcgccg caagcgcacg gtatgatccc tgtctcaacg gcgtcttgga cggcatgtgg
     1081 gcaatgtgcc ggtttaccgc tttatattgc gagtccattc ccggactgcc cgaattgtaa
     1141 ttaatgaatt tccagctggt gatcgttccg tcctttttga ccccggcggt aacatcgatt
     1201 acgccgccag gccggaaata agcccaggta aattcttctt cccgcgtcca gaccagcttg
     1261 accggcttac cgacagcctt tgccagcctg gctgcctcaa tggcggcctc gccgctatgt
     1321 ttccccccat agccggaccc tgtatccgga acgataaccc ttgctttgtc cttgtcgagc
     1381 ctgaaggtgt tgatcagctc ctcctgaact ccgaaaggcc gctggcttcc tgtccaaacc
     1441 gtgagcttcc cgtctcgcca ttcggcaaga gcggcccttg gctcgagcgg agtatgggca
     1501 atgtaatgga tataataggt ttgagaatgc cggaaatcgg cctgcgccag accggccgcc
     1561 acgtctccat tcgtatctgc gtctctttcg ccacttgcgt tcttcacgag gtaatcaaaa
     1621 atgtcggcat tggatggctg gcccgcctct tcctgccaat tcgcattaat cagcatcaac
     1681 gccttttcgg cggtgaccag gtcggttgcc gcgacgccga taaagtcccc atcccgaacg
     1741 acgcttactc cctgtaaggc ctttgctttg gagatgtcgg cagataccag ttttgcgttg
     1801 taggacggtg cccgcagaac ctttccgtac aacatacccg gtacgtgcat gtccgacgtg
     1861 taggcatgtt tgccgctaat gtaagcgctg ccatctactt tgggaaccga ctttcccgaa
     1921 atcttccagt ccttcgctgc aaccacggga acgtcgtccg atatggtgcg aaggatatcc
     1981 tgaccctttg tgaggtcgcc atagctcagc cggacattgg cagcggtatt aacgatcatg
     2041 ccgttttccg catgcagtgc ggcgggctgc gcgtcccagc tcttggcggc catttctatc
     2101 agggcttcgc gagccgtagc cgcggccctg cgaagttggg tgcccatttg cggagtggtc
     2161 cggctgccga acgtgccggc atcgtaggga acaagatcgg tatcgcccat gatcattttg
     2221 atcgatgata ccggcacctt caattcctcc gccacgatct gtgacagcga cgtgcgaata
     2281 ttctgaccga attcgacttt accggtgtag acgctgatcg atccgtcctg gccgatatgt
     2341 atccaggcgc ccacctcggt ggtcggaacg gtgggcgacc catggcccgg cctgcctcct
     2401 gcgaacgaca ggaaatcgtt gaagacaaag gcaacgacca ggccacctcc ggtgagcttg
     2461 aggaatttgc gacggttgat tgtgggagga ttgtccatcg tcgaatgttt atgatccgga
     2521 agcgagctta atggctctta cgataagagg ataggttccg cagcggcaga tattgccgtt
     2581 catagccttt acgattgcat cttcagaagg agccggattc gagttaagca gcgccactgc
     2641 ggacatcagc atcccggatg cgcagtatgc gcactggaag acgtccgtcg tgagaaaggc
     2701 ctcttgtacc ttgtggagct ttccatcctt ctccaggcct tcgatggtgg taatatcctt
     2761 gcccgcgagc gcgttgatgg gcgtaatgca ggcatgtgtt ggtttcccgt tgaccagaac
     2821 ggtgcaagcg ccgcaggcgc ccacgccgca gccatatttg ctgccggtca gttggaggtc
     2881 gttgcgcagt acggtcaaaa gtgcaacatc agggtctgcc gtgatctcgt gtcggacgcc
     2941 gtttacgttt aagaccatga cttatgattg aatcggtaag tgaatgtacg aaaatttgct
     3001 cacatgcgag cgggcattaa tatagccccg gggaggcgta cgcatgcatc tatagcctgc
     3061 ttgcaggggg tcaccggtca ttcaataccc tcatcgtcag gctatcgata tatacaattt
     3121 tatttttctg cggccggaga tagaagtcct gcccgatctc aacggtatcc tgggcaaacc
     3181 gcttgacgat gatcggttgc cggtccagag agcaataaat gatctgctca ttgcccatat
     3241 tttcaaacgc tactgctttc gctttgacag ccgaaggccg gccgggttcg actatgtccc
     3301 aatgctcggg tctgatcccg atttccataa cttcgcccgt ttttgcctgc aggctggcgg
     3361 ggacggaatc cccgagaaga atggatcggt cggccgaaac ccgaagctgg tattggcccg
     3421 atgatttgtc caggacgccc cggatgaaat tcatttccgg tgagcctatg aagcccgcta
     3481 caaatttatt ttccggcttg ttgtagatat tcagcggcgt gtccagttgc atgagcttcc
     3541 ccttttgcag caccgcgatc ctgtttccaa gggtcatcgc ttctacctgg tcatgcgtga
     3601 catacaccat ggtcgcgttg atctcgcggt gcagtttttg caattcaatc cgcatttggc
     3661 tgcggagttt ggcgtccagg ttgcttagcg gttcgtcaaa caggaaaatt tttggccttc
     3721 tcacgatggc cctccctatt gcgacgcgtt gccgctggcc gccggaaagt gtcctgggct
     3781 tcctgttcag ctccgctgaa atgtccaaca aacctgccgc ctgcatgacc ctttccctga
     3841 tctcagtctt gctgtaatgt ctcaatctta gcccaaaagc catattgtcg tacaccgtca
     3901 tatgcggata taatgcataa ttttgaaaaa ccatcgcgat gtcgcgttcc ccgggtcccg
     3961 cttcatttac ccttatcccg tcgatcgaga ggttgccggc gctgatgtct tccaggccgg
     4021 ccatcatccg gagcagggtt gatttgccgc agccggaggg tccgacgatc accatgaatt
     4081 ccttatcccg gacggtgaag gaaacatcgt caatcgcctt ctttggatcc tggccccgcc
     4141 cttcggcggc gtataccttt gatatgtgtt gcaagctaag ctccgccata aaagaaattc
     4201 ttttcgatga ctgtgatccg gcaagcgagg tccgattcgc ccgacgctat aatatactgg
     4261 tccttatgcg cgacaatacc ggtactgaac actacatcat gcaagtacat ttgatggctg
     4321 atgggcgcgg tcagctccgg agatgcctcc agcaggggct cctggtcttc caaccggagg
     4381 atcgtctggg gatcgtcccc gtccaggatc gcccaaaaag ttctgtagca tcctatttcg
     4441 ccattgtttt cgactccgtg atataacatc agccagccat cgcggctccg taggggcgga
     4501 gtaccgcccc cgacctttag gttggcggcg gaagactttc tggccctgag aaatggttca
     4561 ttggaaggct tccaataaag gaggtcaggc gataacgcaa ggtggatggc ggcgccggga
     4621 tgataggcac tctgatgcgg cgacgcgaaa taacattcac ccatgggcct ggttagcgcg
     4681 taatacaaat tattcgcttt gccttc
//
LOCUS       DATGKE010000416         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_333078, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000416 DATGKE010000000
VERSION     DATGKE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333078"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VKU83_07190"
     CDS             complement(<1..1208)
                     /locus_tag="VKU83_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HLI93375.1"
                     /translation="MLNYPVRIALIAALGGFLFGFETVVISGAEQTLEHLWHLSKLAH
                     GITVGSSMYGTFLGALVAGFPAKRYGRKRSLQVIAVMFIVAALGCALTPAWGWFICFR
                     IIGGLAVGGSSVIAPMYISEISPAKLRGRLTGSFQVNIVTGIVVAYLTNFLISQLQLP
                     DAWRWMLGIMVVPAVIFFALLFAIPESPRWLILANDEARARPILERLGEVNIAAAVNA
                     IRDSVKQHQEKLFRRRYSKPIVCAMLLAAFNQLSGINAILYYAPRIFGMAGFDRSQSF
                     SQSIFIGVANLVATLLAMTVIDKLGRKTLLIIGSIGMIVFLSLVAGAFSGDFSGNSHL
                     LLYLVGFIVFFAFSQGAVIWVFISEIFPNSVRSQGGSLGSTTHWGMAAIITTVFPTIV
                     EGHKDGGFWS"
     gene            complement(1290..>2175)
                     /locus_tag="VKU83_07195"
     CDS             complement(1290..>2175)
                     /locus_tag="VKU83_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI93376.1"
                     /translation="ALATGIFNSGANIGAVVAPIMVPVILASYGWQMAFIITGSLGFV
                     WLICWLAFYEIPSRSKRVGKAEYAYIHSDDEQAEIGKAKVPWARLLRVRQTWAFIFGK
                     FLTDPIWWFFLFWLPDYFHKSFHLNLAKPGWPLVIIYSATTVGSIGGGYFSGALIRRG
                     WPVYRARKTVMLIFAICVVPVFAVQYFSSEWMVVALISLAAAAHQAWSANIFTTCSDM
                     FPKKAVSSVTGIGGMAGSIGGIIFQPMVGWILDYFTRTRNATLGYDFIFTLCGAAYLV
                     AWVVMHLFAPKMARVKLD"
BASE COUNT          544 a          650 c          586 g          395 t
ORIGIN      
        1 aaggaccaga acccgccatc cttatggcct tcgacgatcg tggggaatac ggtcgtgatg
       61 atcgcggcca tgccccagtg ggtggtgctg cccagggaac cgccctgcga ccttaccgag
      121 ttagggaaga tctctgaaat gaacacccag atcaccgcac cctgggaaaa ggcgaagaag
      181 acgataaatc cgaccagata aagcagcaaa tgactgttcc ctgagaaatc cccggaaaat
      241 gcaccggcga ccaggctcaa aaagacgatc atgccgatgg acccgatgat cagcaaggtc
      301 tttcggccca atttgtcgat aacggtcatg gccagcagcg tagcgacgag gttggcaacc
      361 ccgatgaaga tggactggct gaacgattgg ctccggtcaa aacccgccat cccgaagatt
      421 cgcggtgcat agtacaggat cgcgttgatg cccgacaatt gattgaatgc cgccagcagc
      481 atggcgcaca ctatgggctt gctatagcgg cgccggaaga gtttctcctg gtgctgcttc
      541 acggagtcgc ggatcgcatt gaccgcggcg gcaatattta cttctccgag tctttccagg
      601 atcggccggg cgcgggcttc gtcattcgcc agtatcagcc agcgcggact ttcagggatg
      661 gcgaacaaca gcgcgaaaaa tatcaccgcc gggaccacca tgattcccag catccatcgc
      721 caggcatcgg gcagttgcag ctggctgatg agaaagttgg tgagataggc caccactatc
      781 ccggtcacga tattcacctg gaaggatccc gtaagtctgc ccctgagctt cgccggcgag
      841 atctccgaga tatacatcgg cgcgatcacg gatgaaccac ctaccgccaa acccccgatg
      901 atgcgaaagc agatgaacca gccccaggcc ggcgtaaggg cacagccgag cgccgccacg
      961 atgaacatca cggcaatgac ctgcaaggat cttttcctgc cataccgctt cgccgggaac
     1021 cccgccacca gggcgccaag gaatgtgccg tacatacttg agcccacggt tatcccgtga
     1081 gcaagcttgc tcaggtgcca taagtgttcc aatgtctgtt cagccccgga tatgacgacg
     1141 gtttcaaaac cgaagaggaa tccccccaga gcggcgatca gtgcgatgcg gaccggatag
     1201 ttaagcacgg caaacgattt aggtaagccg aaaatacagc tattttgatt cccggtttaa
     1261 atgaaatccg gcagtccggc cggcaccccc taatccagtt tcacgcgcgc catcttcggt
     1321 gcaaacaggt gcatgacgac ccacgctacc aggtatgcag ctccgcagag cgtaaagatg
     1381 aaatcgtagc ccagcgtggc attccgggtc cgcgtgaaat aatcgaggat ccagcccacc
     1441 atcggctgaa agatgatgcc gccgatcgac cctgccatcc cgccgatccc cgtcaccgag
     1501 ctgaccgcct ttttggggaa catatcggag caggtagtga aaatattcgc gctccaagcc
     1561 tgatgcgccg cggctgccag gctgatcagt gctacgacca tccattcact gctgaagtat
     1621 tggacggcaa aaaccggcac gacacagatg gcaaagatca gcatcacggt ttttcgcgcc
     1681 cggtataccg gccagccccg cctgatcagg gcgcctgaaa aataaccgcc gccaatactg
     1741 cccaccgtcg tagcggagta gatgatgacc agcggccaac ccggtttcgc gaggttcaaa
     1801 tggaaacttt tatgaaaata atccggcagc cagaagagga agaaccacca gatcgggtcc
     1861 gtcaggaact tgccgaagat aaaggcccat gtctgccgga cgcgaagcaa cctggcccac
     1921 ggcactttgg cttttccgat ctctgcctgt tcgtcgtcgc tgtggatata ggcatattcc
     1981 gccttgccga cccgcttcga gcgggaaggt atctcataaa aagccaacca gcagatcagc
     2041 caaacgaaac ccagcgagcc cgtgataatg aatgccattt gccagccata ggaagccagg
     2101 atcaccggca ccatgatcgg tgccaccacc gcgccgatat tcgcacccga gttaaagatt
     2161 cctgtggcaa gcgcc
//
LOCUS       DATGKE010000417         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_882910, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000417 DATGKE010000000
VERSION     DATGKE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882910"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..347)
                     /locus_tag="VKU83_07200"
     CDS             complement(111..347)
                     /locus_tag="VKU83_07200"
                     /inference="COORDINATES: protein motif:HMM:NF002149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLI93377.1"
                     /translation="MSDIATRVKKIIVDKLGVEEAEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAETITTVGQAIAYLEEHAK"
     gene            498..926
                     /locus_tag="VKU83_07205"
     CDS             498..926
                     /locus_tag="VKU83_07205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI93378.1"
                     /translation="MALRMIDHGTKEYQQMVQLRSDMLRKPLGLTFDKDELEREKDDI
                     LMGAFEDDKLLGCCLMTKMDPATLRLRQMAVPNSMQGKGVGRALMIFAENIARDLGYK
                     KLCMHARSTAVGFYQKLGYHVSGDEFIEVTIPHFVMEKDL"
     gene            complement(927..1280)
                     /locus_tag="VKU83_07210"
     CDS             complement(927..1280)
                     /locus_tag="VKU83_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HLI93379.1"
                     /translation="MAEDYPLFKPSADDRLLAMLSHLLALVYGVGVLGPLVIYVLKKN
                     QSEFVMENAKESLNFQLTVLILACVCLFLVIIYIGLGLLWVLEIVNVVQVIIATVKAN
                     ENKIYRYPINFRLVK"
     gene            complement(1327..>2824)
                     /locus_tag="VKU83_07215"
     CDS             complement(1327..>2824)
                     /locus_tag="VKU83_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HLI93380.1"
                     /translation="KTFSDRLRDFPVKVEFINRFKSAKDKKETLKKLEAGEINIIIGT
                     HALLGKDVKFKDLGLLIVDEEQKFGVAHKEKIKTLRTHVDCLTLTATPIPRTLHFSLM
                     GARDLSIINTPPSNRQPIQTEVHGYNEDFIRDAIYYETERGGQVFFIYNRVQGLAEMA
                     AIIQGLCPDLSIGYAHGQMEGHDLEDRILHFIDRKYDVLVCTNIVESGVDIPNVNTII
                     VNNAHHFGLSDLHQLRGRVGRSNKKAFCYLIAPSLATLPADSRKRLQTLEQHSDLGSG
                     FQIAMRDLDIRGAGNLLGGEQSGFMVEIGFEMYQKVLDEAIRELKRTEFRELFKEEIA
                     KQEDYVQDCTIDTDLEILIPDDYVESITERLSLYTRLDNCDTEAELVALHGEMIDRFG
                     PVPPQVEDLFDTVRIRKLAVSLGFEKLILKQDDLRCYFIDRPDSPYFESDAFRQVLEY
                     IQKHTNKAKLKQAGKHFLLVVDDIRSMRDLLAFLRRMGNFVLADGIPA"
BASE COUNT          645 a          726 c          760 g          693 t
ORIGIN      
        1 cgcggcccaa gtgttttaag gaataaatcc gccttctaag ccccagtgct tgaaaaggcg
       61 gatccaacga atttattgca gagaccagaa attacgattg cagtttcagc ttacttggca
      121 tgctcttcca ggtaagcaat ggcctgaccc acagtggtaa tggtctcagc ctgttcatcc
      181 gggatggaga tattgaattc cttttcaaac tccatgatca gttcaacggt gtccagtgaa
      241 tctgcaccga gatcattggt aaaagacgcc tcgttcgtaa cttcggcttc ttccaccccg
      301 agcttgtcca ctataatttt cttgactctt gtagcgatgt ccgacataca aatttgattt
      361 ttattagtta tacggctgca aatgtaaaaa aattgggcta aaccaaacaa acatggcttt
      421 tttttattta gctacccccg gaaaactgac atttttaata acttcggcct cccggccaaa
      481 aagggctaaa aaacggtatg gcgcttagga tgatcgacca tggaacgaag gaataccagc
      541 agatggtaca attgcgcagc gacatgctgc gaaagccgct ggggctgacg tttgacaagg
      601 atgagctcga acgggaaaag gacgatatcc tgatgggcgc ttttgaagac gacaaattgc
      661 tgggttgttg cctgatgacg aagatggatc cggctaccct ccggcttcgg cagatggccg
      721 tccccaacag catgcagggc aaaggcgttg gccgcgcgct gatgatcttt gcggaaaaca
      781 tagcccgcga ccttggctat aaaaagctat gcatgcatgc ccggtcgacc gccgtcgggt
      841 tctaccagaa actcggctat catgtctcag gcgatgaatt catcgaagtg acgatcccgc
      901 actttgtcat ggagaaggat ctctgattat ttgaccagcc tgaaattgat ggggtaacgg
      961 tagatcttgt tctcattcgc cttgacggtt gcaatgatta cctggacgac gtttactatt
     1021 tccaataccc acaacaatcc gaggccgata tagatgatca ccaggaaaag acaaacgcag
     1081 gcaagtatca gcacggttag ctggaaattg agcgactcct tggcattctc catgacgaat
     1141 tcggactgat tttttttcag gacgtagatc acgagcggac cgaggacgcc aacgccgtaa
     1201 acaagcgcca gcaggtgcga cagcatggcg aggaggcggt catctgccga aggcttgaac
     1261 agcggataat cttctgccat gttcgggtca tttaggcgcc ctgaaggtaa ctgaattttg
     1321 gctcgttcac gcaggaatcc cgtccgccag cacaaaattc cccatccgcc tcaggaatgc
     1381 caggaggtct cgcatcgagc ggatgtcgtc caccaccagc aggaagtgct tgcccgcctg
     1441 cttgagcttt gccttattcg tatgtttctg gatatactcg aggacctgcc gaaacgcgtc
     1501 cgactcgaaa tacggcgagt ccgggcggtc gataaaatag cagcgcaggt catcctgttt
     1561 gaggatcagc ttttcgaacc cgagactgac cgcaagtttg cggatccgga cggtgtcaaa
     1621 aaggtcttcc acctgcggcg ggaccggccc gaacctgtcg atcatttccc cgtgcagggc
     1681 gaccagctcg gcttcggtgt cgcaattatc cagccgggtg taaagggaaa gtcgctccgt
     1741 gatgctttcg acgtaatcgt cggggatgag gatctcgagg tctgtatcga tggtacagtc
     1801 ctggacatag tcttcctgtt ttgcgatctc ttccttgaaa agctcccgga attcggttcg
     1861 cttgagttct ctgatcgctt cgtccagcac cttttggtac atctcgaagc cgatttccac
     1921 catgaagccg ctttgctcgc cgccgagaag gttgccggcg ccgcggatat cgaggtcccg
     1981 catggcaatc tggaagccgc tgccgaggtc gctgtgctgt tcgagggttt gcaggcgttt
     2041 ccgggaatcc gccggcaagg tggccaggga gggcgcgatc aggtagcaga aggccttttt
     2101 gttgcttcgt ccgactcgtc cccgaagctg gtgcaagtcg ctcaggccga aatgatgcgc
     2161 attgttgacg ataatggtgt tgacattggg gatgtccacg ccgctttcca cgatgttcgt
     2221 gcataccagc acatcgtatt tccggtcaat gaagtggagt atcctgtcct ccaggtcgtg
     2281 gccctccatc tggccatgag catacccgat gcttagatcg gggcaaagcc cctggatgat
     2341 cgcagccatt tccgccagac cctggacgcg gttgtaaata aaaaagacct ggccgccacg
     2401 ttcggtttca taatagatcg catcgcggat aaaatcctcg ttgtagccat gaacctcggt
     2461 ctggatcggc tggcggttgg aaggcggagt attgataatg ctcaggtccc gggcgcccat
     2521 caggctgaag tgaagcgtcc ggggaatcgg cgtcgcagta agggtcaggc aatccacgtg
     2581 agtacggagc gttttgatct tttccttgtg ggccactccg aacttctgtt cctcatccac
     2641 gatcaacagt cccaggtcct tgaactttac gtcttttccg aggagcgcat gggtgccgat
     2701 gatgatattg atctcgccgg cctcaagttt cttcagggtt tccttcttgt ctttggcgga
     2761 tttaaaccgg ttgataaatt cgacctttac cgggaagtcg cgcagccggt cggagaatgt
     2821 tttg
//
LOCUS       DATGKE010000418         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_947794, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000418 DATGKE010000000
VERSION     DATGKE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947794"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VKU83_07220"
     CDS             <1..212
                     /locus_tag="VKU83_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93381.1"
                     /translation="HDSEHHFWQFGNQPVDLDKMGQKGRPVPSPIDKLMLAKWVDDPA
                     HYIYCSLYPRQRVKLTPDHSCINKK"
     gene            337..933
                     /locus_tag="VKU83_07225"
     CDS             337..933
                     /locus_tag="VKU83_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93382.1"
                     /translation="MQSNDKPIVPLDACYFLTLNTVDKIDLFVRPAYKEIIADALNHF
                     IRQQGVQLYAWCLMTSHLHLVLRTKDASAPAYFERDFKKFTTPALLKAIETEMDLRRD
                     WMLDRFEAYGKFLRRIEKYHLWQNCSSPLRIDPQQPGLLMDRIVHVHENPVREGIVDQ
                     PEAYLFSSARDYSGIRGLVDVRVVPSQGLFRVKRLSAN"
     gene            1000..1527
                     /locus_tag="VKU83_07230"
     CDS             1000..1527
                     /locus_tag="VKU83_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI93383.1"
                     /translation="MQHPATQEAFVRLVNDHRGMLFKVCRLYCTAEADRQDLFQEIVV
                     QLWRSYPAFRGDSTTGTWLYRIALNTAITGLRKRKRAIALASTDPDRLPAGLEDIPYN
                     MEKEEQLKLLYSAISQLTEVEKALTMLYLEDKSYQEMEDILGISQNNLRVKMNRIKEK
                     LRKSTQAFAYGIGKS"
     gene            1508..2137
                     /locus_tag="VKU83_07235"
     CDS             1508..2137
                     /locus_tag="VKU83_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93384.1"
                     /translation="MELENLKYIWYSLEVPRPDRDSGELVALLRRRSRGPVASMIRNL
                     AGEAIALIIAYVPAILCFLLDFKGRLAAVSWLFLVLLAVFLAYYYRKYRLLREMQCPA
                     CQVRSNLSRQVATLKKYTGFYLLAGTLTIPVTYLIAYFIIRWRPGPAGSAFYYRLHPV
                     AWWASPAFILVLLVPLTIGMYYANAWCIHKLYGRHIKKLQDLLFEMDAE"
     gene            2163..2603
                     /locus_tag="VKU83_07240"
     CDS             2163..2603
                     /locus_tag="VKU83_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HLI93385.1"
                     /translation="MIRRSAIMLASLFILALTGLAQQKPSPDEAAIRRIMANQVDAWN
                     RGNLKAFMEGYWKSDSLVFIGRSGPTYGYHQALANYRKNYNGPDQMGKLFFDLLKVKR
                     LSADYYFVIGKWFLKRRAGDTGGVYTLLFRKIDGQWRIVVDHSS"
     gene            complement(2605..3378)
                     /locus_tag="VKU83_07245"
     CDS             complement(2605..3378)
                     /locus_tag="VKU83_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HLI93386.1"
                     /translation="MAYKTRKSRAWKWLLLLPGGAVAFGVGWLGYVWLMERRAHFMNY
                     AAFGTEVPVNYSIHGIDVSKYQDYIDWGMVKGMKVGDVQMSFVFIKATEGLNNEDGFF
                     NRNWKKSRDAGLCRGAYHYFLATRSGKAQAENFISLVDLMPGDLPPVLDIEQSYGVPP
                     DKLRQRAKEWLQTVEDYYHVVPIIYTNVDFYNQFLKQDFDGYPLWVAHYLQKNRPNIY
                     RDWAFWQHSEGGRVNGIVTRVDFDVFNGDSTDFRRLLIN"
     gene            3450..>3663
                     /locus_tag="VKU83_07250"
     CDS             3450..>3663
                     /locus_tag="VKU83_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93387.1"
                     /translation="MPGFSRHDTINFLSKMTFPRFFNGVKVLSSFYLSKWTRRPIQWG
                     YPVSLSFEPTTACNLRCPECPSGLRAF"
BASE COUNT          835 a         1050 c          935 g          843 t
ORIGIN      
        1 agcacgactc cgagcaccat ttttggcaat tcggcaatca gcccgtcgat ttggacaaga
       61 tgggccaaaa aggcagaccg gtcccttcgc cgatcgataa gctgatgctg gcaaaatggg
      121 tcgatgaccc ggcgcactac atctattgca gcctttaccc ccgtcaacgg gtaaaactga
      181 cacccgatca ttcgtgcatt aacaaaaaat aaagtgaacg gcccgggatg gaaggccggg
      241 atttttgtaa ctttaaacca atccccaccc atacgtgttt ccatgacacc cggcggcttt
      301 tcgcaggggc cgacttgttc ataaaaactt gtgcgtatgc agtcgaatga caaaccaatc
      361 gtcccgctcg atgcgtgtta ttttttgacg ctcaatacgg tagataagat cgatttattt
      421 gtccggcccg cttacaaaga gattatagcg gatgcgctaa atcattttat ccggcagcag
      481 ggggtgcaac tgtatgcctg gtgcctgatg accagccacc ttcacctggt cctgcggaca
      541 aaagatgcgt cggccccggc ctatttcgaa agggatttca aaaaatttac gacgccggcg
      601 ctgttgaaag cgatcgagac ggaaatggac cttcgccggg actggatgct ggaccgcttt
      661 gaggcatacg ggaaattcct cagaagaatc gaaaaatacc atctctggca gaattgcagc
      721 agccccctgc ggatcgatcc ccaacaaccc gggctgctca tggaccgcat cgtacacgtt
      781 cacgaaaacc ccgtgcggga gggaatagtc gatcagccgg aagcctatct tttcagctcc
      841 gcccgtgact acagcggcat ccgcgggcta gtcgacgtcc gggtggtccc gtctcaaggc
      901 ctgttccgcg tgaaaaggct gtccgccaat tgatatgaag gtgaacgtaa tattttcgcc
      961 caacctgcga caaacacagc aaccctcaaa ttttcgcatg tgcagcaccc tgcgacacag
     1021 gaagcctttg tcaggctggt gaacgaccac cggggtatgc tcttcaaggt ttgccggctg
     1081 tattgtaccg ccgaagcgga ccggcaggac cttttccagg agatcgtcgt tcaattgtgg
     1141 cgctcctacc cggcttttcg cggggattcg acgaccggca cctggcttta ccggatagcc
     1201 ttgaacacgg ccatcacagg tcttcggaaa cggaagcggg cgatcgccct ggccagcacc
     1261 gatccggatc gactgccggc aggccttgag gatatcccgt acaacatgga aaaagaagag
     1321 cagctcaagc tgctgtattc cgcgatcagt caattgacgg aagtcgaaaa agcattgacc
     1381 atgctttacc tggaagacaa gtcctaccag gaaatggagg acatcctggg tatttcacaa
     1441 aataacctgc gggtcaagat gaaccggatc aaggaaaaac tacgtaaaag cacccaagca
     1501 ttcgcttatg gaattggaaa atcttaaata tatctggtat tcgctggagg tcccgcggcc
     1561 ggaccgggac tccggtgagc tggtcgcgct tctccgaagg aggtcccgcg gcccggtcgc
     1621 cagcatgatc cgcaacctgg ccggcgaggc gatagccctg atcattgcct atgtgcccgc
     1681 catcctttgt ttcctcctgg actttaaagg caggctggcc gcggtttcgt ggttgttcct
     1741 ggtcctgctg gcagtttttt tggcctatta ctaccgtaaa taccggctgt tgcgggaaat
     1801 gcaatgcccc gcctgccagg tcaggtcgaa cctttcccgc caggtcgcga cgctgaagaa
     1861 atataccggg ttctatctcc ttgccggcac gctcacgatc ccggtcacct atctgatcgc
     1921 ctacttcatc atccggtggc gtccgggccc ggccggatcg gccttctatt accggctgca
     1981 tccggtggcc tggtgggcca gccccgcgtt tatcctcgtg ctgctggtgc cgctgacgat
     2041 cggcatgtat tacgcaaacg cctggtgcat ccacaaatta tacggcaggc atatcaaaaa
     2101 attacaggat ttgctttttg aaatggatgc agagtgagta atttaccgga aaatccccgt
     2161 ccatgatccg acgctccgcc atcatgctgg cctcgctatt cattctggcc ctgaccggtc
     2221 tcgcccagca gaaaccctcc cctgatgaag ccgcaatccg ccgcattatg gccaatcagg
     2281 tcgacgcctg gaatcgcggg aacctgaagg cctttatgga gggctactgg aagagcgatt
     2341 cgctggtctt catcggccgg tcgggcccga cctacgggta ccaccaggcg ctcgctaact
     2401 acagaaagaa ttataatggt cctgaccaga tgggaaagct gttcttcgac ctcctaaagg
     2461 ttaaaaggct gtccgccgat tactactttg tgatcgggaa atggttcctg aaaagaagag
     2521 ccggggatac cggcggcgtc tatacccttc tcttccgcaa gatcgacggc cagtggcgga
     2581 tcgttgtcga tcacagcagc tgattcaatt gatcagcaat ctcctgaaat cggtggaatc
     2641 cccgttaaag acatcaaaat cgacccgggt gacgatcccg ttcaccctgc caccctcgct
     2701 atgctgccag aacgcccagt cccggtaaat attgggcctg ttcttttgga gataatgtgc
     2761 cacccacaac gggtacccgt cgaagtcctg tttgaggaac tgattgtaga agtcgacatt
     2821 ggtgtaaatg atgggaacga catgataata gtcttccacg gtctgtaacc attctttcgc
     2881 ccgttggcgc agcttgtccg gcggcacgcc gtacgactgc tcgatgtcaa gcacgggggg
     2941 cagatcgccc ggcatgagat cgaccaaact gataaaattc tccgcctgcg ctttgccgct
     3001 cctggttgcc agaaaataat ggtaggcgcc ccggcacagc cctgcgtccc gggatttctt
     3061 ccagttccgg ttgaaaaagc catcttcatt gttcaagcct tcggttgcct tgatgaatac
     3121 aaagctcatc tgtacgtcgc cgactttcat tcccttcacc attccccagt cgatataatc
     3181 ctggtatttc gaaacgtcga tcccgtggat gctgtaattc accggaacct cggttccgaa
     3241 tgcggcataa ttcatgaaat gtgcccgccg ctccattagc cagacgtagc caagccaacc
     3301 cactccgaag gctaccgctc cccctgggag caataataac cacttccagg ccctgctttt
     3361 ccttgtttta tatgccatac ttcattcagc ccgcggctcc gcgaccgtct ccggcgtgta
     3421 caaacataat ttagtatttt tatatttcca tgccgggctt tagccgccac gatacgatca
     3481 atttcctgtc gaaaatgacc tttccgcggt tcttcaacgg ggtcaaggtg ctgagcagct
     3541 tttatctcag taagtggaca aggcggccga tccagtgggg ctatcccgtt tccctttcct
     3601 ttgaaccgac gaccgcctgc aacctgcggt gcccggaatg cccgagcgga ttgcgcgcat
     3661 tta
//
LOCUS       DATGKE010000419         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_13118, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000419 DATGKE010000000
VERSION     DATGKE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13118"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1377
                     /locus_tag="VKU83_07255"
     CDS             184..1377
                     /locus_tag="VKU83_07255"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HLI93388.1"
                     /translation="MNRVYTHLFIINFLIVSIPRVAFSQCNCSVGVPATPITYYQSFG
                     PTNASTTNLSFPQFNPSVGTLACMSVWDTASGITATSALNKDLFDSVTYAFLLSLTPS
                     LTGPGGVNISQSYNKTYGPTTLAPLGHPGDTVTYGPDTIFNNVPGSKTNVSNTAPYLG
                     TGNVNFGFSISGGVLTTEGGGNYTAGPTTNYWGAVRLTYYWCPAAPLSVNITDFTVTP
                     QGNMLLLQWLTQDQQPNTQYEIQISQDGKSFYDIGQAEGDASATGAAAKYQYQYNPDP
                     AYVGQLYFRIRQTDPSGKVSISMVLVVDPRTGRSGGAVSYQTFPNPATNSLQIQFNGS
                     QSGRFLMQLIGTSGQVVQEQLVTLAGSSQIRMDLSPHPVPGLYFLRTTDQTHNRSYVT
                     KVFVQ"
     gene            1543..2247
                     /locus_tag="VKU83_07260"
     CDS             1543..2247
                     /locus_tag="VKU83_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HLI93389.1"
                     /translation="MEYNTTRNHLVMREYGRHIQKMIEYLVSLEDKEKRQRNAHAVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLISDFRLEVASPYPIPTRETLRARPKPLKYPKRY
                     PKFSHLGKNLELVINKALAEENPDKRAGFANAVAYYMKLAYNNWHKETVHDDAIQTEL
                     TNLTNGQLEFTATPFVKSFRPEDNRQARDRDNRGNTFGGDYRNKRNQKFQQRSGNRNN
                     ERNNDRGGNKFKKKRF"
     gene            2256..>3110
                     /locus_tag="VKU83_07265"
     CDS             2256..>3110
                     /locus_tag="VKU83_07265"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLI93390.1"
                     /translation="MHSFEVIGGKKLKGEIIPQGAKNEALQIISAVLLTPEKVTISNI
                     PDILDVNLLIDLLEEMGVKIDRKERHICTFQADAVDIDYVRSEAYRKKSGRLRGSVMV
                     AGPMLARFRKAFIPKPGGDKIGRRRLDTHIIGFEKLGAQFRYESEEGLFHLDAPQLAG
                     CSMLLDEPSVTGTANIIMAAVMAEGTTTIYNAACEPYIQQLCRMLTRMGAAISGIGSN
                     LLKIEGVSYLSGTEHRMLPDMIEIGSFIGLAAMTQSDITIKHAAVDQLGIIPEKFRQL
                     GIRLETAGD"
BASE COUNT          775 a          847 c          813 g          675 t
ORIGIN      
        1 atccaccacc cggaaaaatc aaagcgttga aagtcaataa gctgagtata acaaatgatc
       61 gccaatgatg tggtttttac ggtgcttaca aattgagtag tagtgctttg gcctcattct
      121 tgcagcttag taaatttgta gtattaatcc gaatagaatc cgacctatta aaaattcgtg
      181 cctatgaacc gtgtgtacac tcatttattt attatcaatt ttttgatagt aagtatcccc
      241 cgggtggcct tctcgcaatg taactgtagc gtcggggtcc ccgccacccc cattacgtat
      301 taccaatctt ttggtcctac caacgcttct acgacgaatc tgtcttttcc acaattcaat
      361 ccatccgtag gcacgctggc ctgtatgagt gtttgggata ccgcctcggg cataaccgcc
      421 accagcgccc tgaacaagga ccttttcgac agtgtcacct atgcgttcct gctgtccctg
      481 acgccttcgt tgacgggacc gggcggagtc aatatctccc aatcgtacaa caagacctac
      541 ggacccacga cgctggcgcc gctgggtcat ccgggcgata ccgtcactta cggaccggat
      601 accattttca ataacgtccc gggcagcaag accaatgtct ccaatacggc gccttacctg
      661 ggaacgggga acgtgaactt cggcttcagc atcagcgggg gtgtcctgac cactgagggc
      721 ggcggcaact atacggcagg tcctaccacc aattattggg gagcggtccg tctcacctat
      781 tactggtgtc cggcagcccc tctctccgtg aacattaccg attttacggt tacgcctcag
      841 ggaaatatgc ttttattaca atggttaacc caagatcaac aacccaatac ccaatatgag
      901 atccaaatca gccaagacgg caaaagcttc tatgacatcg gacaagcaga aggtgacgct
      961 tctgcaacag gtgctgctgc aaaataccaa tatcaataca accctgatcc ggcttatgtg
     1021 ggacagctct atttccgcat ccggcaaaca gatccttcgg ggaaagtgag catttcgatg
     1081 gtgctggtgg tcgacccccg taccggtagg tctggcggcg ccgtgagcta tcagaccttc
     1141 cctaacccgg ctaccaacag cttgcagatc cagttcaacg gcagtcagtc cggccgtttc
     1201 ctgatgcagc tgatcggcac gagcgggcag gtcgttcagg agcagttggt caccctggcc
     1261 gggtccagcc agatcaggat ggacctcagc ccgcatcccg ttccgggtct gtatttcctg
     1321 cggacaacgg accagacgca taaccggagc tatgtgacca aggtgttcgt tcagtgataa
     1381 ttcctatgag agtatatgtc gtatcccatt agttaatgcg atgccggctt gggttccaac
     1441 aaaaagtacc cggccggcat tctcttatga tcgaccaggc gcaagtctga cattagcgtt
     1501 tttgaggttt ttactgtaac ttgcccttct aaagcaattt taatggaata caacaccacg
     1561 cggaatcacc tggtgatgcg tgaatatggg cggcatatcc agaagatgat cgaatacctg
     1621 gtgtcgctgg aggataagga aaaacggcag cgcaatgcgc acgccgttat cgagctgatg
     1681 gggtttctga atccgcacct gaagaacgtg gaggatttcc ggcacaaatt atgggaccac
     1741 ctgttcctga tctccgattt ccggctggag gtggcctccc cctatcccat tcccacacgg
     1801 gaaacgctgc gggcacggcc caaaccgctc aaatacccca agcgttatcc gaaattctcg
     1861 catttgggca agaacctcga gctggtgatc aacaaggcgc tggcggaaga aaatccggac
     1921 aagcgcgccg gtttcgccaa tgcggtagcc tactacatga agcttgccta taacaactgg
     1981 cacaaggaga ccgtccatga cgacgctatc cagactgagc tgaccaatct caccaacggt
     2041 cagctcgagt ttaccgcaac accgttcgtg aagagcttcc gcccggaaga caaccgtcag
     2101 gcacgcgacc gggataaccg gggcaacacc tttggcggcg attaccgcaa caaaaggaac
     2161 cagaagttcc agcagcggag cggcaaccgc aataacgagc ggaataacga ccggggcggc
     2221 aataaattca aaaagaaaag attctaacta agagtgtgca ttcattcgaa gtcatcggcg
     2281 gtaaaaaact gaaaggagaa atcatcccgc agggagccaa gaacgaagct ttacagatca
     2341 tcagtgcggt gctgcttacg ccggagaagg tgacgatctc caacattccc gacattctgg
     2401 atgtgaacct gctcatcgat ctgctggaag agatgggggt gaagatcgac cgcaaggagc
     2461 ggcacatctg tacttttcag gccgatgccg tggatatcga ttacgtgcgg agcgaagcct
     2521 atcgtaagaa gagcggccgg cttcgcgggt cggtgatggt tgcaggtccg atgctcgccc
     2581 gtttccgcaa ggcatttatc ccgaagccgg gcggcgataa gatcggccgg agacggctcg
     2641 acacccatat catcggtttc gagaaattgg gcgcgcagtt ccgttatgaa tccgaagaag
     2701 gtcttttcca cctggatgcg ccacaactgg cgggctgttc catgttgctg gacgagccgt
     2761 cggtgacggg tacggctaac atcattatgg ccgcggtcat ggcagaaggc acgactacga
     2821 tctacaatgc ggcatgcgaa ccatacatcc aacagctgtg ccggatgctc acgcggatgg
     2881 gcgccgctat cagcggcatt ggcagtaacc tgctcaaaat agaaggggtc tcttatcttt
     2941 cgggcaccga acaccggatg ttaccggata tgatcgagat cggctccttc atcggtctgg
     3001 cagccatgac gcaaagcgac atcaccatca agcatgcggc agtcgatcag ctggggatca
     3061 tcccggaaaa gttccggcag ctgggcatcc ggctcgagac ggcgggcgac
//
LOCUS       DATGKE010000420         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_923162, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000420 DATGKE010000000
VERSION     DATGKE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923162"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VKU83_07270"
     CDS             complement(<1..876)
                     /locus_tag="VKU83_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HLI93391.1"
                     /translation="MKMSIKGIHHITAIAGNARRNLDFYTRVLGLRMVKRTVNFDDPG
                     TYHFYYGNESGTPGTILTFFPWEGITAGRTGTGMATEIGYSVSQESLAFWAERFREQG
                     IGHGPVSARMGETYLSFRDPDGLQISLIAAAEPDDRRPWSTPEVPAAVATKGFHSVTL
                     TLRDIVPTAELLTEVFGYQAERQDGNRFRFRTDTVPTAAIVDLVEATAAGRGLNGGGT
                     NHHVAFRVSDEETQLAFREKILSRGLHVTPKINRDYFFSVYFREPGGVLFELATDNPG
                     FTVDEPLAQLGTALKL"
     gene            1010..2164
                     /locus_tag="VKU83_07275"
     CDS             1010..2164
                     /locus_tag="VKU83_07275"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI93392.1"
                     /translation="MEYDNDRLRIRTTPAETFCWLLIGFLGPVTDSIAILHVDPPGWF
                     LAALISGLSFPLYLLYSYALGTAFVVRRQKWLRLALYFLAFFMIGAFILTLHYLLSKF
                     DLRPVERAYLYPATPTLYRVGGWVALNLALATRAFHIRDALEQKEELEAARKDSSFFQ
                     LRYLRAQLNPHFLFNTLNSIYSLSLQKSARAPELVVRLADIMRYLIYECDEETIPLDK
                     EIEFIRNYIEIEKVRHQADIRLTVEGLTAGVRIEPFLFISFIENGFKHAMENSFSEPY
                     IYITIKVASGLITLNVINSVDADLESQAKRINGKGIAHSRSLLELLYPSAYDLDIIQT
                     EKDESRTSRLRLRNARERLENLYPDSHTLDVILNKSSFTVSLILKTAPAC"
     gene            2158..2862
                     /locus_tag="VKU83_07280"
     CDS             2158..2862
                     /locus_tag="VKU83_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93393.1"
                     /translation="MLKCIIVEDEALAQDVLKSHLDRLGSLELAGVYRSAREAEAVLR
                     TREIDIMFLDIRLPGMSGLQFLSGLQEAPLVVLTTAYAEYALESYEFNVIDYLLKPIS
                     FERFSRAVNKVLDNRIFTTAAKDADPFPADHIFVRSNSKFFRVNFSEILYVQGNKDYL
                     KIHTPDYSLITHQTMNDLEQTLPSRQFIRVHKSYIVALRHIRAIYGNSIELGKVTIPI
                     GVSYKDAVMTLVGRKH"
BASE COUNT          618 a          882 c          759 g          621 t
ORIGIN      
        1 caatttaagc gccgtcccca actgggcgag gggctcgtcg acggtgaaac cgggattatc
       61 cgtggcgagc tcgaacagga cgccgcccgg ttcgcggaaa taaaccgaaa agaaatagtc
      121 ccggttgatc ttcggcgtaa catgcaggcc gcggctaagg atcttttccc tgaaagcgag
      181 ttgcgtctcc tcgtcggaaa cccggaatgc cacatgatga ttcgtaccgc cgccattgag
      241 gccgcggccc gctgccgtcg cctccacgag gtcgacgatc gccgcagtgg gaacggtgtc
      301 ggtcctgaac cggaacctgt ttccgtcctg gcgctctgcc tggtacccga aaacctccgt
      361 gagcagctcc gccgtcggta ctatgtcgcg tagcgtcagg gtcacactgt ggaacccttt
      421 cgtcgcgaca gcagccggga cttccggtgt gctccagggc cgccggtcat ccggctccgc
      481 ggcagcaatc aggctgatct gcaacccgtc ggggtcccgg aacgaaagat aggtctcccc
      541 catcctcgcg gaaaccggcc catgtccgat cccctgttcc cggaagcgct ccgcccagaa
      601 ggcgaggctc tcctgactaa ccgaataccc gatctccgtc gccatgcctg ttccggtccg
      661 gcctgcggtg atcccctccc aggggaaaaa ggtcaatatc gtcccgggcg taccgctctc
      721 attcccgtaa taaaaatgat aggttcccgg gtcatcgaaa ttgaccgtgc gtttgaccat
      781 ccgcaggccc agcacccgcg tataaaaatc gagattgcgc cgcgcgttgc cggcaatcgc
      841 ggtgatatgg tgtatgccct taatgctcat tttcatggcc taaaggtacg cgcatttcca
      901 gcgggcggcc gcttgcagag caaattgatc gaaacctggg cgaaatttgt cgaaaagttt
      961 tccggtcctt gccgcggaat agtagtttta tggcctaagc tgttccgaca tggaatacga
     1021 taacgaccgg ctccggatcc gaaccacgcc cgcagaaacg ttttgctggc tgctgatcgg
     1081 tttcctcggc ccggtcaccg acagcatcgc catcctgcat gtcgacccgc cgggttggtt
     1141 cctcgcggcg ctcatcagcg gactaagctt cccgctctat ctcctttatt cctacgccct
     1201 gggaaccgct ttcgtggtcc ggcgccaaaa atggttgcgg ttggcgctgt atttcctcgc
     1261 tttcttcatg atcggcgcat tcatcctgac cttacactac ctgctgtcga aattcgacct
     1321 ccggccggta gagcgcgcct acctttaccc ggcgacgccg acgctttacc gcgttggcgg
     1381 gtgggtcgcg ctgaaccttg cgcttgcgac gcgcgctttc catatccgcg acgcactgga
     1441 acagaaggag gagttggagg ccgcccgcaa ggatagcagc ttctttcagc tccgctatct
     1501 gcgcgcccag ttaaatccgc attttctctt caatacgctg aacagcattt actccctgag
     1561 cctccaaaaa tcagcgcggg cgccggagct cgtcgtccgc ctggctgata tcatgcgcta
     1621 tctcatttac gagtgcgatg aagagaccat tccgctcgac aaggaaattg agttcatccg
     1681 gaattacatc gaaattgaaa aggtccgcca ccaggcggat atccggctta ccgttgaagg
     1741 gctgaccgcg ggtgtacgga tcgagccctt cctgttcatt tccttcattg aaaacggctt
     1801 caagcatgct atggagaaca gcttcagcga accatacatc tatatcacca tcaaggttgc
     1861 cagcgggctc atcaccctga acgtcatcaa cagcgtggat gccgacctcg aaagccaggc
     1921 caaacggatc aatggaaagg gcatcgcgca tagccggagc ctgctggagc tgttataccc
     1981 gtccgcctac gacctcgata tcatccaaac cgaaaaggat gagagcagga ccagccggct
     2041 gcgcctgcgc aacgcgaggg aacgcctgga aaatctatat cccgactccc atacccttga
     2101 cgtaatttta aacaagagct cattcacggt gtcactcatt ctaaaaaccg cgcctgcttg
     2161 ctgaaatgta tcattgttga agacgaggcc ctcgcccagg atgtactgaa gagtcatctc
     2221 gaccgcctcg gaagcctgga gctggccggg gtgtaccgca gcgcccggga agcagaggcc
     2281 gtactgcgga cccgcgagat cgatatcatg ttcctggaca tccggcttcc cgggatgagc
     2341 ggcctgcagt tcctcagcgg acttcaggaa gcaccactcg tcgtccttac gacggcttat
     2401 gccgaatacg ccctggaaag ctacgagttc aatgtgatcg attatctttt aaaaccgatc
     2461 tctttcgaac gtttttcccg ggcggtgaac aaggtcctgg acaaccggat cttcacgacg
     2521 gcggcgaagg acgcggatcc cttcccggcg gaccatatct tcgtccgctc gaacagcaaa
     2581 tttttccgcg tgaatttttc agagatcctc tatgtccagg gaaacaagga ttatctgaag
     2641 atccatacgc cggattactc gctgataacc catcagacga tgaacgacct ggaacaaacg
     2701 ctgccatcgc gccaattcat cagggtgcat aaatcgtaca ttgttgcgct ccgccacatc
     2761 cgggcgatct acggcaactc catcgagctc ggcaaagtca cgataccgat cggcgtcagt
     2821 tataaggatg ccgtcatgac cctcgtcggc cggaagcact gagcttattt tccctatttt
//
LOCUS       DATGKE010000421         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_367868, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000421 DATGKE010000000
VERSION     DATGKE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367868"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..301
                     /locus_tag="VKU83_07285"
     CDS             80..301
                     /locus_tag="VKU83_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93394.1"
                     /translation="MAPYQIWQWGGHILDTHMGSQSSRIAAFDMGFDFTGFEIDSDYF
                     IAGNKRFAEHTAQAQLFTDPDLYTTGPEY"
     gene            331..753
                     /locus_tag="VKU83_07290"
     CDS             331..753
                     /locus_tag="VKU83_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93395.1"
                     /translation="MNKTMFADVPPDQRQQLLADNCDGHEKTSYMKDLTQEDIDVKNE
                     TISSNCIKVFELEEELKQIKDSYKERISPLKDETRELCGQVKTRKEQVNGILFHFADH
                     ENSVMNTYDENGEFVSSRRLRPEEKQARLFVGHKAVNE"
     gene            784..1509
                     /locus_tag="VKU83_07295"
     CDS             784..1509
                     /locus_tag="VKU83_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93396.1"
                     /translation="MKSELTVTPKGDTINILEGTALEPKHPNKLKIVGQVQSVNHYMA
                     ARRGTTQPHGALQHIDRDLAVISIDEEAMTIHLDVDPNNPFGTEVLGKLEINPDLVPF
                     GINKQVEFTRDKLIHLLRFSRRFFIHPSQHETLLAAYQKLNITGSTNLKAETDTRGNK
                     DLGFKKTIDSSTIPTEFTLEMPVFKGQPNRKFRVEICLDATDASVRFWFESVELVEII
                     EKDKKEIFQKQIADYLDYAIIWK"
     gene            1739..>2477
                     /locus_tag="VKU83_07300"
     CDS             1739..>2477
                     /locus_tag="VKU83_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HLI93397.1"
                     /translation="MREPLFIVIDLFCGFGGTTRGFELTELDGHGIAKVFACVNHDPK
                     AIKSHWLNHPDVTHYEEDIRTLDLTELVKLTAEVRCRYPNSKLVLWASLECTNFSKAK
                     GGLPRDADSRTLADHLPRYITALQPDYIPIENVVEFMSWGPLDSMGKPVSRKSGEDWL
                     RWRKAINALGYIDEWQELNSANYGAYTSRNRLFGIFARPELPICWPAPTHAKNPSKLS
                     MYGDLKKWMPVREVLNFEDEGESILSRE"
BASE COUNT          679 a          637 c          634 g          527 t
ORIGIN      
        1 aaggaactgt catgcagggc aacaaacgat tgaatgagaa gcgcattcac cccacacaga
       61 aacccgtcac cctctacaaa tggctcctta ccaaatatgg caatgggggg ggcatatcct
      121 cgacacgcac atggggagcc agtccagcag gatcgcggct ttcgatatgg gtttcgactt
      181 cacgggcttc gagatagatt cagattactt tattgcgggc aacaagcgct tcgcggaaca
      241 taccgcgcaa gcgcagctat ttaccgatcc ggatttatac acgaccgggc cggaatatta
      301 agaaactttt ttaacctaaa ataaaattca atgaacaaga ccatgtttgc ggacgtcccg
      361 ccggatcagc gccaacagct actcgccgac aactgcgacg gccacgaaaa gaccagctac
      421 atgaaggatc tgacgcagga ggatatcgac gtcaagaacg aaacgatatc ctccaattgc
      481 attaaggtct tcgaactgga ggaagaattg aagcagatca aggacagcta taaggaaagg
      541 atttccccac tgaaggatga gaccagggag ctttgcggcc aggttaagac taggaaggag
      601 caagtgaatg gcatcctatt ccatttcgcc gatcatgaga attccgtcat gaacacctat
      661 gatgaaaacg gcgagttcgt cagcagccgg cgcctacgtc ccgaagaaaa acaggcccgt
      721 cttttcgtcg ggcataaggc cgtcaacgag taaacaccat ttcccacatt ttaacaattt
      781 gacatgaaat ctgaactgac agttacccct aaaggcgaca cgatcaatat tttggaagga
      841 acggcgctgg agccgaaaca ccccaacaaa ctcaagatcg ttgggcaggt acaatccgtc
      901 aaccactaca tggctgcaag gcgcggcacc acgcaacctc acggcgccct ccagcacatc
      961 gaccgagatc tggccgtgat aagcattgat gaagaggcta tgaccattca cctggacgtc
     1021 gatcccaaca accctttcgg tacggaagtg ctgggcaaac tggagatcaa ccctgacctc
     1081 gttccttttg ggatcaataa gcaggtcgag tttacccgcg acaaactgat ccatctgctg
     1141 cgctttagcc gccggttttt tatccatccc tctcaacatg agaccctcct ggcggcatac
     1201 cagaagctca atatcaccgg cagcacgaat ttaaaggccg agaccgatac gcgcggcaat
     1261 aaggacctgg gcttcaaaaa gaccattgat agctctacca taccgacgga atttactttg
     1321 gaaatgcctg tgttcaaggg acagcctaat cggaaattcc gggtggaaat ttgcctggac
     1381 gcgacagacg cctctgtccg cttttggttt gaatcggtcg agctggtgga aatcattgag
     1441 aaagataaaa aggaaatctt tcaaaagcag atcgccgatt atctggatta cgccatcatc
     1501 tggaagtaat gatacgccgc tcatccctta caataaccta ctccgacctg gtaaaatttg
     1561 ccgagaaaca ggcctcctac gcccgtcaaa gaagaaattc agcatcaggt aggggagtag
     1621 tgaactgcga ggggcttacc catgacgtgg aatgcgcgga gatgttggtg aggatgttga
     1681 ggaaggggat gccggggcgg caaacttcct tttatgaact ttttgaactg gctaataaat
     1741 gagagaaccg ttatttattg ttatcgatct tttctgcggt tttggcggga cgacccgtgg
     1801 attcgagctc accgagcttg acggccacgg tattgccaag gtcttcgcct gcgtgaacca
     1861 tgaccctaag gccattaaga gccattggct taaccacccc gatgtaacac attatgagga
     1921 agatatccgt accctggatc ttaccgagct tgtgaagctg accgcagagg tgagatgccg
     1981 atacccgaat tcgaagttgg tcctatgggc atcgctggag tgcaccaact tcagcaaagc
     2041 caagggtgga ctgccgcgcg acgccgatag ccggacattg gccgatcatc ttccccggta
     2101 tataaccgcc ctgcagcccg attacatccc cattgagaat gtcgtcgaat tcatgtcctg
     2161 gggaccgctc gacagcatgg gcaagccggt aagccggaaa agcggcgaag attggctccg
     2221 ctggcgtaag gctatcaatg cactgggtta catcgacgaa tggcaggaat tgaatagtgc
     2281 taactatggg gcgtatacat ctcgcaaccg tttgttcggc atcttcgccc ggcctgaact
     2341 gccgatctgc tggcccgcgc cgacgcacgc caaaaatccc tcgaagctgt ccatgtatgg
     2401 cgatctgaaa aagtggatgc ccgtgaggga ggtattgaac tttgaggacg aaggagaaag
     2461 tatcctgtcc cgtgaga
//
LOCUS       DATGKE010000422         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1365292, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000422 DATGKE010000000
VERSION     DATGKE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365292"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VKU83_07305"
     CDS             <1..521
                     /locus_tag="VKU83_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI93398.1"
                     /translation="NIYALTEVDAALAGKAIHQLSRMMRYLLYDTQPGSTMLSQEIAF
                     VRDYIGLMKLRLTETATVDIQTPADLRDMPIAPMIFLPFIENAFKHGVRPLEPSQIKI
                     VISQSGSLLDLTVRNPIINDNRTSLDAGTGIGLVNTRRRLDLLYPGKFKLDIGNPGPD
                     NAFTVHLVLDLS"
     gene            518..1270
                     /locus_tag="VKU83_07310"
     CDS             518..1270
                     /locus_tag="VKU83_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93399.1"
                     /translation="MIIHCIAVDDEPLALGLVCKFIGQTPFCKLAGRYASAVEALKSL
                     HAGKIDVIFLDIQMSDLNGIELARVLGKGPAAPLIIFTTAYNQFALEGYKLDVVDYLL
                     KPFNYEEFLRAATRAYDRVKLRAKAAPGPGAPELAPGAAKPDEQFLFLKVEYQLVRIA
                     LDDILYIEGLKDYIKVHRKTSDKALMSLTSLKALEDRLPPKRFMRVHRSFIVALDKIT
                     SMTRNSVQIGKTVITVGDQYKAAFSKFIGKWI"
     gene            1346..2104
                     /locus_tag="VKU83_07315"
     CDS             1346..2104
                     /locus_tag="VKU83_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLI93400.1"
                     /translation="MKKLLVCLLCVTTASLVHAQSSRFSREKYVEKGDTLYYRLLFPD
                     ANRQRKYPLVVFLHGSGERGSDNEAQLKWGVMNFATDRNMLEHPAIVIAPQCPEHETW
                     SSVTRERDGRGMTLNAEPSKPMRLLIGLIRQLQTTMPVDTERIYITGLSMGGYGTYDA
                     LERYPRMFAAAVPVCGAGDTSRAASIAHIPMWIFLGAEDPAVNPLNGLAMLEALTNAG
                     AHPGYTQYPEVGHFSWLGAYSDPLMMEWLFRQHK"
     gene            2115..>2484
                     /locus_tag="VKU83_07320"
     CDS             2115..>2484
                     /locus_tag="VKU83_07320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI93401.1"
                     /translation="MERAKQIAQETVFSILFTISLAHLINDMLQSVVPAVYPLIKDTY
                     HLTFTQIGLITLTYQLTASLLQPLVGWYTDRRPRPYSLAMGMGFSCLGVFAVAMAGSF
                     VQLLFAVSLIGVGSSIFHPES"
BASE COUNT          548 a          687 c          707 g          542 t
ORIGIN      
        1 ataatatcta cgcgctgacc gaggtcgacg ccgcccttgc cggcaaggcc atccatcaat
       61 tgtcccggat gatgcgctat ttgctctatg acacgcagcc gggatccacg atgctgagcc
      121 aggagatcgc ctttgtgcga gactatatcg gcctgatgaa attacggctg acagagacgg
      181 caactgtcga tatccagacg ccggcagacc tcagggatat gccaatcgcc cccatgatat
      241 ttctgccctt tattgaaaat gcattcaaac acggggtaag gcctttggag ccgagccaga
      301 tcaaaatcgt aatttcgcag tcaggtagcc tgctcgacct gaccgtgagg aatccgatca
      361 taaacgataa ccgtacgagc ttggacgccg gtaccggcat tggcctcgtg aataccagac
      421 ggcggctcga cctgctgtat cccgggaagt tcaagctgga catcgggaac ccgggcccgg
      481 acaacgcttt tacggttcac ttagtgcttg acctgtcatg atcatacatt gtattgcggt
      541 agatgatgag ccgcttgcgc tcggactggt ttgcaagttt atcgggcaaa cgcccttctg
      601 caagctggcc ggccggtatg cgagcgccgt ggaggcattg aaatcactcc atgccgggaa
      661 gatcgacgta atctttctcg atattcaaat gtccgatctg aacggcatcg agctcgcgcg
      721 cgtattgggc aaaggtcccg ccgcgccact catcatcttt accacagcct ataatcaatt
      781 tgcactggag gggtataagc ttgacgtggt cgactatttg ttgaaaccgt tcaattacga
      841 ggaattcctt cgggcggcaa ccagggctta cgaccgggtt aagttgcggg cgaaggccgc
      901 tcccgggccg ggagcgccgg aactggcgcc cggggccgcg aaaccggatg agcaattcct
      961 ttttctaaag gtggagtatc aactggtgcg aatcgcgtta gacgacattc tttatattga
     1021 ggggctgaag gactacatca aagtgcaccg gaaaacctcc gataaggcgc ttatgtcgct
     1081 caccagcctg aaggcattgg aagaccggct tcccccgaag cggttcatgc gggttcaccg
     1141 gtcttttatc gtcgcgctgg acaagatcac ctcgatgacc aggaactccg ttcagatcgg
     1201 caaaaccgtg atcacggtcg gtgaccagta taaggctgca ttcagcaagt ttatcgggaa
     1261 atggatctga gccctgtggg gcgggaccac aggcctaatt tcgcgcctgg cccgcgttta
     1321 acaaaaatcc cctaaattcg gcatcatgaa aaaattgctt gtttgtctcc tctgcgtcac
     1381 gacggccagc ctggtccacg ctcaatcgtc gcggtttagc cgggaaaaat atgtcgaaaa
     1441 gggcgatacg ctatactatc gcctgctgtt tccggatgcc aaccgccaaa gaaaataccc
     1501 gctcgtggtc tttttacatg gctcggggga acggggtagc gacaacgagg cgcagctgaa
     1561 gtggggcgta atgaacttcg cgaccgaccg gaatatgctg gagcacccgg cgatcgtcat
     1621 cgcgccgcag tgccccgagc acgagacgtg gagcagcgta acgcgggaaa gggatgggag
     1681 agggatgacc cttaacgccg agcctagcaa gccgatgcgc ttgctgatcg gccttatccg
     1741 ccaattgcaa acgacgatgc cggtggacac ggaacggatc tacattaccg gattgtcgat
     1801 gggcgggtac gggacttacg atgcgctcga acgctatccc aggatgtttg ccgcagccgt
     1861 accggtgtgc ggagccggcg acacgtcgcg ggccgcatcc atcgcccata tcccgatgtg
     1921 gatcttcctg ggcgcggagg acccggcggt caaccccttg aacggcctgg cgatgctgga
     1981 agcgctgacc aacgccggcg cccatcccgg ttacacccaa tacccggagg tcgggcattt
     2041 ttcgtggctg ggcgcctata gcgacccact gatgatggag tggttgttca gacagcataa
     2101 gtaagccaag ggagatggag cgcgccaagc aaattgctca ggaaaccgtt ttttcgatcc
     2161 tatttaccat cagcctggcc cacctgatca atgacatgct tcagtcggtg gtgccagcgg
     2221 tttaccccct gataaaggat acgtaccatc tgacctttac gcagattggc ctgatcacgc
     2281 tgacctacca gttgaccgct tcattgctcc agccgcttgt cggctggtat actgaccgca
     2341 gaccccggcc ctactcttta gcgatgggga tgggattcag ttgcctggga gtgtttgcag
     2401 tagctatggc cggcagtttt gttcagctgc tgttcgcggt cagccttatc ggcgtgggtt
     2461 catcgatatt tcacccggaa tcct
//
LOCUS       DATGKE010000423         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_539438, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000423 DATGKE010000000
VERSION     DATGKE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539438"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VKU83_07325"
     CDS             <1..187
                     /locus_tag="VKU83_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93402.1"
                     /translation="LSTSAANTETDAFSLLRADILAIQAEIAAALPKVTDRMTRFHLQ
                     DIQVRIRKTLEANPTIQ"
     gene            308..1267
                     /locus_tag="VKU83_07330"
     CDS             308..1267
                     /locus_tag="VKU83_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLI93403.1"
                     /translation="MANISLTTVKYYIGVTDNTWYSFLAQKNRDDVNFWQPGGNKVFR
                     ALPPGGPFLFKLKSPVNAIGGLGFFSSHTFLPLSMAWEVFGEGNGSSTLSQLSRMISD
                     YRNDGLGNPTIGCIVLTNPIFFDKSDWIGIPENWSRNIMQGKAYSTQDRIGAELWSKV
                     ENLLQNYLPVAPEKKTSQLIVEEEAAGYGNPFLTRIRLGQSAFRMLVTDAYNRRCTIS
                     GEKTLPVLEAAHIKSYAESGPHVVSNSLLLRSDLHRLFDAGYLTITTDLKVEISKRIK
                     EEFENGREYYKFHGNNISNLPNRGLDLPARNYVLWHNENVYLD"
     gene            complement(1311..2132)
                     /locus_tag="VKU83_07335"
     CDS             complement(1311..2132)
                     /locus_tag="VKU83_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93404.1"
                     /translation="MNKAKSRVRVVPILKRKKRARVPIPASEGKERIALFEGDVPSPP
                     KNAQLAYGGGALIQNASVYTIFWSSSWSGNTNMQTLMGQVNRFFTDILVSPLIDQLSE
                     YNVAGHYAIGHGNLAGTTVITAGAPASGTSIDDAAIQKALQGWINGNQVPAATPDRLY
                     FIYFDQGVTVTMEGGSSCSDFCGYHDAINNNIYYAIMPYPSCDGCLAGLSIVDAITGT
                     SSHELCEAITDPVPPNGWYDQTNNMEIGDICAWTFKRVAGHNVQLEWSNQAGKCV"
BASE COUNT          535 a          568 c          541 g          520 t
ORIGIN      
        1 gctatctacc agcgcggcca ataccgagac cgacgccttt tcactgttga gagcggacat
       61 cctggcgatt caggccgaga tcgccgcggc cttgccaaag gtcacggacc ggatgacgcg
      121 gttccatctg caggatatcc aggttcggat caggaaaacg ctcgaggcca atccgacgat
      181 acagtagggc cagcgctgga gctgaacatt ccacgattat ttttcggaac agtcgcgtta
      241 ccagtaacta agaaccagct gccagctaat tgccgctaac cgccgaagcc gttaaacttt
      301 tagtatattg gccaatattt ctcttacaac agtgaaatat tacattggcg taaccgataa
      361 cacttggtac tccttccttg ctcaaaagaa tcgagacgac gttaattttt ggcaaccggg
      421 aggcaacaag gtgttcaggg ctttacctcc gggagggcca tttcttttca aattgaagag
      481 tcccgttaac gccattggcg gacttgggtt cttttcgagt catacctttc tgccgctttc
      541 aatggcctgg gaggtctttg gcgaaggcaa tggttcaagt acgctgtctc aactgagccg
      601 catgatatcg gattatcgaa atgacggcct cggaaatcca accatcggat gtattgttct
      661 taccaatcct attttttttg acaaaagcga ttggatcggc attcctgaaa actggagcag
      721 gaacataatg caggggaagg cttattccac ccaagaccgg attggcgcag aactttggtc
      781 aaaggtcgaa aacctgttgc aaaattatct tccggtggct cccgaaaaaa agactagtca
      841 actcattgtt gaggaggaag ctgcaggcta tggcaatcca ttcttaacca gaatcaggct
      901 tggccagagt gcgtttcgaa tgcttgttac cgacgcatat aaccgtcgat gtaccatttc
      961 aggggaaaaa accttgccgg ttctcgaggc agcccatatc aaatcatatg cagaatcggg
     1021 cccgcacgtc gtctccaatt ctcttttgct tcgatctgac cttcaccgac tgtttgacgc
     1081 ggggtatctg acgataacca cggacctaaa agtcgaaatc agcaaaagga taaaggaaga
     1141 attcgaaaac ggcagagaat attacaaatt tcacggaaat aatatctcca accttccaaa
     1201 caggggacta gacctacctg caagaaatta cgttctatgg cacaatgaaa atgtgtatct
     1261 ggactgagca aaaacagctc agttcgtcgt gctctgctga cggcgggcat tcacacgcat
     1321 ttccccgcct gattgctcca ctccagctgc acattatgcc ctgccaccct cttaaatgtc
     1381 cacgcacaga tatcacctat ctccatatta ttggtctggt cgtaccaccc gttcggcggc
     1441 accggatcgg taatcgcctc acaaagctcg tgggagctcg ttccggtgat cgcatcgaca
     1501 atcgataggc ccgcaaggca gccgtcgcag gaaggatagg gcatgatcgc gtaatagata
     1561 ttattgttga tggcgtcatg atagccgcag aagtcggagc aggaactgcc gccttccatg
     1621 gttacagtca ccccctggtc gaaataaatg aaataaagcc tgtccggcgt cgcagcaggc
     1681 acctggtttc cgttgatcca gccttggagc gccttttgaa tagcggcatc gtcgatgctg
     1741 gtacccgaag ccggcgcgcc ggccgtgatc accgtcgttc cggccaggtt gccatgaccg
     1801 atcgcgtaat gtcccgccac attgtactcg ctgagctggt cgatcagggg gctgacgagg
     1861 atatcggtaa agaaccgatt gacctggccc atcagggttt gcatgttggt gttccctgac
     1921 cagctgctgc tccaaaagat cgtatagacg gaggcattct ggatcagcgc cccgccgcca
     1981 tacgccagct gcgcgttctt cggcggcgat gggacatcac cctcgaaaag tgcaatgcgc
     2041 tctttccctt cggaagcggg gatgggaacc ctggcgcgct tcttcctctt aaggatgggt
     2101 actacccgca cgcggctttt tgctttgttc ataacatgag atttattggt caatgattaa
     2161 tcgt
//
LOCUS       DATGKE010000424         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1389926, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000424 DATGKE010000000
VERSION     DATGKE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389926"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VKU83_07340"
     CDS             <1..691
                     /locus_tag="VKU83_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLI93405.1"
                     /translation="KKIKWGNIWLFAVIFLLAGAGSELARKNNWPNRRPLNLFENTYR
                     FGSLVFGGGNVLVPMMYEQFVARPQSQKLLQRNPHVIRIDKAEFYSGAGMVRAIPGPT
                     FSVASYMGGVAMKDRGSAWQVLGCFIGATGIFLPSALLVLFFFPIWHNLKKYAVVYRA
                     LEGINAVVVGIIIASALYMMKDLWQNNWTEIGIGLGVIAATWALLSLTRLPSPVIVLI
                     WLALGWLVMVL"
     gene            complement(614..2017)
                     /locus_tag="VKU83_07345"
     CDS             complement(614..2017)
                     /locus_tag="VKU83_07345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLI93406.1"
                     /translation="MTFKEFGLDDSLVEGIDASGYENATPVQEQVIPLILQGRDIIAS
                     AQTGTGKTAAFLLPIIHKLIQHSNTDTVNALVIVPTRELAIQIAQNMEGLAYFTSISS
                     IAVYGGSDGSNFSTEKQALSRGADVVICTPGRMIAHLNMGYVKLSGLQFLVLDEADRM
                     LDMGFFDDIMKIISFLPAQRQNLLFSATMPVKIRDLARKILHDPAEINIAISKPPARI
                     VQQAYVVYESQKIPLVKWLLKSSNHKTTLIFCSKKHNVKQLTMELRRSKFNVNEIHSD
                     LEQSARENVLLEFKSRRLPVLIATDILSRGIDIDNIEMVINYDVPNDGEDYIHRIGRT
                     ARAETDGKAFTFISEKEQNKFKTIEDLLGHPVNKATLPEEFGKSPEYHPGSRGGKSGR
                     GRRDRGRSSHPERGSRQGGGQQASGNQGSNHQRNSGPQKGGGQHGHPDRGSKHHHQPP
                     QRQPDQNDNRRGQPGQA"
     gene            2196..>3787
                     /locus_tag="VKU83_07350"
     CDS             2196..>3787
                     /locus_tag="VKU83_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93407.1"
                     /translation="MTAEHQRLASNATKKIPLEQWGPYLSERQWGTVREDYSGYGDAW
                     HYFPYDQAHYRAYLWGEDGLGGISDFFQNLCFAVALWNGKDPILKERLFGLGNSEGNH
                     GEDVKELYYYLDNLPTHYYMEYLYKYPQQEFPYTKLKEENRRRTREDPEYEILDTGVF
                     DGEDYFDVTIRYAKQQSRDIFIRIDIHNRHERKADITVLPTLWFHNRWAYDELEKRPT
                     IALRDGNSVRATHSRLGTYYLYYQPPQFTLFTENETNTERVAGVPNKTPFVKDAFHDA
                     IIRRKNLELLRQKKSGTKFAPVYQYTLAGNETRSIYLRLTNKVVDHPFSSDPDQIFID
                     RKKEADEFYNAVFPADIPREMAAVQRQALAGLLWSKQYYHFDVERWLTTGDGITPVNA
                     GKLNGRNHDWKHLKNQDIIAMPDKWEYPWYAAWDLAFQSIAMALVDPVFAKHQLLLIM
                     REWYMKPDGQIPAYEWNFSDVNPPVQAWAAMMVYRIEKHRTGKSDTAFLKRIFQKLLI
                     NFTWWINRKDANGNNMFEGGFLG"
BASE COUNT          853 a          978 c         1097 g          859 t
ORIGIN      
        1 caaaaaaata aagtggggaa atatctggct gtttgcggtc atttttctgc ttgcaggcgc
       61 gggcagtgaa ctggccagga agaataactg gccgaaccgg cggccgttga atctcttcga
      121 gaatacctac cgtttcggca gccttgtttt tggcggtggg aatgttctcg ttccgatgat
      181 gtacgaacaa ttcgtagcaa ggccgcagtc ccaaaaactc ctgcaacgga accctcacgt
      241 tatccggatc gacaaggcgg aattttattc aggcgccggc atggtgcggg cgatcccggg
      301 cccgacgttt tccgtcgcca gctacatggg cggggttgcc atgaaggacc ggggctcggc
      361 gtggcaggta ttggggtgct tcatcggcgc tacagggatt ttccttccca gcgcactgct
      421 cgtgctgttc ttttttccta tctggcacaa cctcaaaaaa tatgcggtgg tgtatcgcgc
      481 actcgaaggc ataaatgccg tggtggttgg gatcatcatc gcttccgcct tgtacatgat
      541 gaaggatctt tggcagaaca actggacgga gatcggcatt ggcctcggag tgatcgctgc
      601 gacctgggcg ctcttaagcc tgacccggtt gccctcgccg gttatcgttc tgatctggct
      661 ggcgctgggg tggctggtga tggtgctttg aaccccggtc cggatggcca tgctggcccc
      721 cgcctttttg gggaccgctg tttcgctgat ggttgctgcc ctggttgccg gatgcttgct
      781 gaccaccacc ttggcgcgaa ccgcgttccg gatggctgga gcgtccccgg tcacgtcgcc
      841 ctcttccgct cttgccgccg cggctccccg ggtggtattc aggtgatttc ccgaactcct
      901 cggggagggt tgccttattc acgggatgac cgagcagatc ctcgatcgtt ttgaatttgt
      961 tttgctcttt ttcgctgatg aaggtgaacg ccttgccatc ggtttcggct cttgccgtgc
     1021 gaccgatccg gtggatataa tcctcgccat cattcggcac atcataattg atcaccattt
     1081 cgatattgtc aatatcgatt ccccgcgaca ggatgtccgt agcgatcagt acaggcagcc
     1141 ggcggctttt gaattccagc aatacgttct cccgggcaga ctgttcgagg tccgaatgga
     1201 tctcattgac gttgaacttc gagcggcgca gctccatggt gagctgtttc acgttgtgtt
     1261 tcttcgagca aaagatgagt gtagtcttgt ggttgctgga tttgagcagc cattttacaa
     1321 gcggaatctt ttgggactcg tacaccacat aagcttgttg cacaatcctg gccggcggtt
     1381 tggagatcgc gatattgatt tcggcggggt cgtggaggat cttccgcgcg aggtcacgga
     1441 tcttgaccgg catcgtcgca gaaaacagga ggttctgacg ctgcgccggc aggaaagaaa
     1501 tgatcttcat aatgtcgtca aagaacccca tgtccagcat ccggtccgct tcgtcaagga
     1561 caaggaactg aaggcccgac agcttcacat atcccatatt aaggtgggcg atcatgcggc
     1621 cgggagtaca gatgacgacg tcggcgccgc gtgaaagggc ttgtttttcg gtcgaaaaat
     1681 tactaccgtc gctgccgccg tacactgcaa tcgagctgat ggaggtaaaa taagcgaggc
     1741 cttccatgtt ttgcgcgatc tggatcgcca gttcgcgcgt gggaacgata acgagggcgt
     1801 tcaccgtatc ggtgttggaa tgctggatca gcttatggat gatcggaagc aggaatgcgg
     1861 cagtcttgcc ggtcccggtt tgagcggaag cgataatatc ccgtccttgt aatatcaggg
     1921 gaatgacctg ttcttgcaca ggggtggcgt tttcatatcc cgacgcgtcg atgccttcta
     1981 ccaggctgtc atcaaggcca aattctttaa aagtcaaaat tcttcttttt caccgcaaag
     2041 ttcggacttt tcggcggaat ttgaacgggg tctggcattt tggcaaaata aagccgccgg
     2101 aactttacta acttcgggct cggaaatgtg ggccgaagga gccggtgctg agcgatgaag
     2161 ccggaagtgg gacagtactg aaacgaaaac caaaaatgac cgcagaacat cagcgactcg
     2221 catcaaatgc gacaaaaaaa ataccgttgg agcagtgggg gccgtacttg agcgaaaggc
     2281 agtggggaac agtacgggaa gattatagcg gatatggaga cgcctggcat tattttcctt
     2341 acgatcaggc gcattaccgg gcctacctgt ggggtgagga cgggcttggc gggatctcgg
     2401 acttttttca aaacctttgt ttcgcagtgg cgctgtggaa cgggaaggat ccgatcctga
     2461 aggagcggct ctttggcctg gggaacagcg aagggaacca tggcgaggac gtcaaggagc
     2521 tctattatta ccttgacaat ctgcccacgc attattatat ggaatacctg tacaaatatc
     2581 cgcaacagga attcccttat acgaagctca aggaggagaa ccgtcgccgg acaagagaag
     2641 atccggaata tgagatcctg gatacgggcg tattcgacgg cgaagactac ttcgacgtga
     2701 cgatccggta cgccaaacaa caatcccgcg atatcttcat ccgcattgat atccataacc
     2761 gccatgagcg gaaagcggat atcaccgtcc ttccaaccct ctggttccat aaccggtggg
     2821 cgtacgatga attggaaaag cggccgacga tcgcgctccg ggacggcaat tccgtgcggg
     2881 ccacgcacag caggctcggg acgtactatc tgtactacca gccgccgcaa ttcacgctct
     2941 ttacggagaa cgagaccaac accgaacggg tcgcgggcgt acccaacaag acgccatttg
     3001 taaaagatgc gttccacgac gccatcatcc ggcgtaagaa cctggagctt ttacggcaaa
     3061 agaaaagcgg aacgaagttc gctcccgtct atcaatatac gctggcggga aacgagaccc
     3121 ggtccattta tcttcggctg accaataagg tggtagacca cccgttttcc agcgatccgg
     3181 accagatctt tatcgaccgg aagaaagaag cggatgaatt ctataacgcc gtgtttccgg
     3241 cggatatccc ccgggagatg gcggctgtcc agcggcaggc gctggcgggc ctgctttgga
     3301 gcaagcagta ttatcatttt gacgtggaac gctggctgac aacgggcgac ggcatcacgc
     3361 cggtgaatgc gggcaaactg aacgggcgga accacgattg gaaacatctc aagaaccagg
     3421 atatcattgc catgccggac aaatgggagt acccctggta tgcggcctgg gatcttgcct
     3481 tccagagcat cgccatggcg ctggtcgacc ccgtgtttgc gaaacaccag ctgctgctga
     3541 tcatgcggga atggtatatg aagccagacg ggcagattcc ggcctacgag tggaatttca
     3601 gtgacgtcaa cccccccgtg caggcctggg cggcgatgat ggtgtaccgg atcgaaaaac
     3661 accggacggg aaagagcgac accgccttcc tgaagcggat cttccagaag ctgctcatca
     3721 acttcacctg gtggatcaac cggaaggatg ccaacggcaa caacatgttc gaaggcggct
     3781 ttctcgg
//
LOCUS       DATGKE010000425         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_53855, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000425 DATGKE010000000
VERSION     DATGKE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53855"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VKU83_07355"
     CDS             complement(<1..1033)
                     /locus_tag="VKU83_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HLI93408.1"
                     /translation="MNVDHRIDLLAELGAYCLSTEPAWLAAQRRAHAANGWFTPEFIG
                     LAVHTIATRFLQRDALRAWAGKYGPHDEPARPVTVGLTMAGNIPLVGFHDFLSIFVSG
                     HRQMIRPSSKDDILIRHLAEHLCRLDPAVAQRVGFADILRGCDAYIATGSNNSARYFE
                     YYFSKYPHIIRRNRTSVALLTGAESRQDLDRLADDVYQYFGLGCRNVTQLYVPENYDF
                     LSLLQAFRKYNYLAQLSKYKHNYDYQLTLLILNKKTYMSNESILLTEDASPFSPISVL
                     HYAYYRRGEALPPALSGGHPDIQCIVGADHLASAAGLSRVPPLVAFGQAQQPALDDYA
                     DGVDTMKFLL"
     gene            1133..1480
                     /locus_tag="VKU83_07360"
     CDS             1133..1480
                     /locus_tag="VKU83_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLI93409.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAIADGTTVKGSFT
                     LLDGTVTDAGARHAPISVDTYYITPNKCTECQGFHEEPQCAAVCPVDCCVPDEMYRET
                     VEELMAKKERLHI"
     gene            1806..>2167
                     /locus_tag="VKU83_07365"
     CDS             1806..>2167
                     /locus_tag="VKU83_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93410.1"
                     /translation="MKPTIAFITGGYSGESVISYKSAVTIENNLDHDRYTVYRIDITP
                     EGWWHALKDGRKIMVDKNDFSLSIDGKRIRFDAVLMGFHGTPGEDGKLQGYFDLLNLP
                     YTSCDAVVSAITFNKRFTV"
BASE COUNT          510 a          517 c          651 g          489 t
ORIGIN      
        1 tcaacaggaa tttcatggtg tcgaccccat cggcgtaatc gtcgagcgcc ggctgctggg
       61 cctggccaaa ggctaccagg ggaggaacgc gggaaagccc tgccgcgctg gcaaggtggt
      121 cggcccccac gatacattga atgtcagggt gaccaccgga cagggcaggg ggcagcgcct
      181 ctccgcggcg gtaataggca tagtgcaata cactgatcgg gctgaacggt gaggcgtctt
      241 ccgtcaacag gatggattca ttggacatat aagtcttttt attgaggatc aggagggtca
      301 gctgatagtc atagttatgt ttgtattttg acagttgggc taggtagtta tatttcctga
      361 atgcctgcaa cagcgagagg aaatcgtagt tttcgggaac gtacagctgc gtcacattgc
      421 ggcagccaag gccaaaatat tgatagacat catcggcaag acggtccagg tcctggcggc
      481 tctccgcgcc ggtcagcagg gcgacagagg tgcggttccg gcggatgata tggggatatt
      541 ttgaaaaata atactcgaaa taccgggcgg aattattgct gccggtcgcg atgtaagcat
      601 cgcagcccct taggatgtcc gcgaatccca cccgctgggc tacggccggg tccagcctgc
      661 agagatgctc tgcaaggtgc cggataagga tatcgtcctt gctcgaggga cggatcatct
      721 ggcgatggcc tgagacgaag atcgaaagga aatcgtgaaa tcccacgagc ggaatattgc
      781 cggccatggt caggccaacg gtaaccggac gggcgggctc atcatgcggt ccgtatttac
      841 cggcccacgc ccgcagcgca tccctttgga ggaagcgggt cgcgatggta tgaacggcga
      901 ggccgataaa ttcgggtgtg aaccagccat tggcggcgtg ggcccgccgc tgggcggcca
      961 gccaggccgg ctctgtcgag aggcagtacg ctccgagttc cgctaataaa tcgatccggt
     1021 ggtctacatt cataaattcc acgctatatt tgccggacgg gctattcgga gtaacgggcc
     1081 cgccaaatcg aacgcaaagg taagtttaat cattccctaa atattcacaa ttatggcgat
     1141 caaaataacg gaagaatgca tcaattgcgg cgcctgtgag cccgaatgtc ccaataatgc
     1201 gatctacgag ggcggagtgg aatgggcgat agccgacggc accacggtca aagggtcgtt
     1261 cacactgctg gacggcaccg tgacggacgc gggcgcgcgg catgctccga tcagtgtaga
     1321 tacctattat attacgccga acaaatgcac cgaatgccag ggttttcatg aggagccgca
     1381 gtgcgcggcg gtttgtcccg tggactgctg cgtcccggat gagatgtatc gcgagaccgt
     1441 tgaagaactg atggcgaaga aggagcggct gcatatataa tgcggcttgc ggcggtgttc
     1501 gggtgcggaa agcatgtatc gaaacggttg aatgcattag atgtgcgaaa gatgtgcgta
     1561 agcgcaaaga tttaatttgg aattgtcgca gaaatcggta actttctacc atcaaattgt
     1621 ttgcgaaagt agccctcgtg ctactgaata taggcgggtg atatttcccg tcatacaagg
     1681 gtgaaggagg tttttccttc acctttcttt ttgtttggcc gcgctggccg cgcgtggttc
     1741 cccggcactc ctgcccttct ttaattgcag cgcgttttgt tattttgcaa aaatcatttc
     1801 gattcatgaa acctaccatt gcatttatta ccggcggcta ttccggtgag tcggtcatct
     1861 cctataaaag tgctgttacg atcgaaaaca acctggatca tgaccggtac acggtataca
     1921 ggatcgatat tacgccggag ggatggtggc acgcgctgaa ggatggcagg aagatcatgg
     1981 tggataagaa tgatttttcg ctcagcatcg acggaaaacg gatcaggttc gacgcagtgc
     2041 tcatgggatt ccacgggaca ccgggcgagg atgggaaact gcagggctat ttcgatctgt
     2101 tgaatcttcc ctacacgagt tgtgacgcgg tggtatcggc gattacgttt aacaaacggt
     2161 ttaccgt
//
LOCUS       DATGKE010000426         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_727969, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000426 DATGKE010000000
VERSION     DATGKE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727969"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..534
                     /locus_tag="VKU83_07370"
     CDS             340..534
                     /locus_tag="VKU83_07370"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLI93411.1"
                     /translation="MNQDQAKGKWNQLKGEIKKKWARMTDDDFLEVEGDMDKLSGRIQ
                     ERTGDKKEDIRRWLNDQNIN"
     gene            663..1025
                     /locus_tag="VKU83_07375"
     CDS             663..1025
                     /locus_tag="VKU83_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93412.1"
                     /translation="MHDRTHTILVVEDEEESRLVLMQILELEGFSTVGASNGAEAMDY
                     LRTSSRPCLIVLDLNMPVMNGRQLRAALWQVEELAAIPVVVVSAQEGFRAQDFGAVGA
                     FRKPLDVDALVKVVVDNC"
     gene            complement(1061..>2604)
                     /locus_tag="VKU83_07380"
     CDS             complement(1061..>2604)
                     /locus_tag="VKU83_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HLI93413.1"
                     /translation="AFVSALIILFVAPLFIDPIIRSSVERDMNEQLKGYETHVAGAHL
                     RLFDGELSLQDVTLIQKAHPRSPVGRFPVMSIDIQWSELFIGHIVADLLLFHPRLRID
                     LTQLRTEEHDKVPLSKKGWQDAIESIYPFRVNHLTIDDADVTYVDTDPKRPLRLEHLY
                     VSAGNIRNLRTPDASYPSPITAEAAVFDVGHASIKGNANFLTKPVASFAVNYHIENVP
                     LQQFRPELQRENLEVEGGTLTSDGFVAYGTAAKRVDVREVRIAGARFGYVHSSPTAAV
                     EARRVAEVKEGTKKINNAPATFIKISRLDIVDSEASFTDETKDPHYRLFISQLRVELT
                     NLSNHFSEGLAHLRLSGLFMGSGLTTLRADFRPEKAGSDFDMDLAMHDASLPSMNDLL
                     RAYGKFDVETGQLSVYSQVTVRQGEMKGYVKPLFSKLKVYDPAQDKNKTAIHKVYEMA
                     VGAAAKVVKNSSTNKVATQVDMSGRLKNPDVSAWQAMVEFVENGFVRAILPGFDRQVR
                     ESASG"
BASE COUNT          600 a          681 c          696 g          627 t
ORIGIN      
        1 ttgccgacga aatcgtggta aaaaaaccct ttacaatgga cgaactcgca cccgccgtgc
       61 gcaaagcctt ggaccgatcc gctggctgag tcgtagtccc ttagcagagg aaatcgacct
      121 cctgcaaatc tgccagtacg aattacgcga tccgactcga gcggttgggc gtggcggccg
      181 cttctttcaa gggcgctgaa aaaccttctt ccccttcaac gcatgggttc aaatcttctc
      241 cattttataa cgtgtttttg atgggcacgc ttgttgctgc ccttcattct tgaacgccac
      301 aaagcaacct ctgctcatgc aggttaaagg ggtatttgaa tgaatcagga tcaagcgaag
      361 ggcaaatgga atcagctcaa gggcgaaatc aagaagaaat gggccaggat gaccgacgat
      421 gatttcctcg aggtcgaagg cgacatggat aagctcagcg gccgaatcca ggagaggacg
      481 ggcgataaga aggaggacat ccgcaggtgg ctaaacgatc aaaatattaa ctagccgtcg
      541 cttcgcggcc gcggcgtcag cgatttttca tatccttgaa ttttcctgac gccgcgccgc
      601 gcttttggtg tggataagtc aaaaattaag atgccatctt acgttttaat ctttaaatga
      661 ccatgcatga tcgcacccat acgatcctgg tggtcgaaga cgaagaagaa tcacgtctgg
      721 ttctgatgca gattctggag ctggaaggct ttagcactgt gggcgcctcc aacggtgctg
      781 aagccatgga ttatttacgt acttcatcgc ggccctgtct catcgttctc gatttgaaca
      841 tgccggtcat gaatggcagg caattgcgcg cagctctgtg gcaggtcgag gagttggcag
      901 ccattccggt ggtcgtagtc agtgcccagg agggttttag agcccaggat tttggtgcgg
      961 ttggagcttt cagaaaaccc ctggacgttg atgctctggt aaaggtcgtg gtcgacaatt
     1021 gttagaacac ctgttagaat acctgttaga atacaggccc tcatcctgag gcggattctc
     1081 gcacctgccg gtcaaatccc ggcaggatag ctcgaacaaa tccgttttcc acaaattcca
     1141 ccatcgcttg ccatgcgctg acatccgggt ttttgagtct tccggacatg tcgacctgag
     1201 tcgcaacttt gttagtcgag gaattcttga ctactttggc ggccgccccg accgccattt
     1261 cgtacacctt atgtatggcg gtcttgtttt tatcttgtgc cgggtcatag accttaagct
     1321 tgctgaacag gggcttcacg tagcctttca tttcaccctg ccgaacggtt acctgcgagt
     1381 acacggaaag ctgacccgtc tcaacgtcaa acttgccata tgcgcgcaaa agatcattca
     1441 tcgatggaag gctggcgtca tgcatggcaa gatccatgtc gaagtccgac cccgccttct
     1501 ccggacgaaa atcggcgcgt agggtggtca acccactgcc catgaacagg ccgcttaaac
     1561 gtaggtgcgc cagcccttcg ctgaaatggt ttgacagatt cgtcaattcg acacggagct
     1621 gggaaatgaa aagccggtag tgaggatcct tggtctcatc ggtaaaggag gcctcgctgt
     1681 caacgatgtc aagcctggaa attttaataa acgtcgcggg agcattattg atctttttcg
     1741 ttccttcctt aacttcagcc acccggcgcg cctccacagc cgcggtcggc gacgaatgga
     1801 catagccaaa gcgcgcgccc gcgatccgga cctcacggac gtcaactctt tttgccgcag
     1861 tcccgtacgc aacgaaacca tcactggtca gcgtgcctcc ctcaacctcg agattctcgc
     1921 gctgaagctc gggcctgaac tgctggagcg gcacgttttc gatatgatag ttcaccgcga
     1981 agctggcaac cggcttcgtt agaaaattag cgttgccttt aatactcgcg tggcccacat
     2041 cgaaaacggc tgcttcggcg gttatgggcg acggatagga ggcgtccggc gtcctcaggt
     2101 ttcttatatt accggccgat acgtacaagt gctcgagtcg aagcggccgt tttggatcgg
     2161 tgtcgacgta ggtgacatcc gcgtcgtcga tggttaagtg gttgactctg aatggataaa
     2221 tgctctctat cgcgtcctgc cagcctttct tggagagtgg aactttatca tgctcctcgg
     2281 tacgcaactg tgtcagatcg atgcgcaatc tgggatggaa caggagcaag tcggctacga
     2341 tatgcccgat gaacaactcg ctccattgta tgtcgatact catgactgga aagcgcccta
     2401 ccggagagcg agggtgcgct ttctggatca gcgtaacatc ctgcaaactg agctctccgt
     2461 cgaatagcct caaatgagcg ccagcgacgt gggtttcata gcctttcagc tgctcattca
     2521 tgtctcgttc gactgacgat cgtatgatcg ggtcgatgaa gaggggcgcc acgaaaagaa
     2581 ttatcaaggc gctgacaaat gccg
//
LOCUS       DATGKE010000427         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1272026, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000427 DATGKE010000000
VERSION     DATGKE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272026"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VKU83_07385"
     CDS             <1..457
                     /locus_tag="VKU83_07385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HLI93414.1"
                     /translation="DKDGNVKNWWTKEDYEKFRAKTKRVIDLYSGFTVLDTVHVKGAL
                     TVGENTADNGGVAIAYDAFKMTPEGKDSSIRIDGFSPDQRFFLSIARIWRLKTRDAYL
                     RMYVNTNPHSPAMWRVNGPLMSREAFYKAFNVQPGDKNYLPDDQRIKIW"
     gene            complement(506..2218)
                     /locus_tag="VKU83_07390"
     CDS             complement(506..2218)
                     /locus_tag="VKU83_07390"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLI93415.1"
                     /translation="MGDSKSRNTFDAIVIGSGMSGGWAAKEFTDSGLKTLVLERGRDV
                     KHLKDYPTTNKMPWEFPHQGQVPLDIQQANPSISRCYAFQEDAMHFFVKDTEHPYVQQ
                     KPFDWIRGYQVGGKSLMWARQTQRWSDFDFEGPARDGYAVDWPIRYKDLAPWYSHVEK
                     FAGISGNRDGLDQLPDGEFLPPLDLTCIEDHFRQVVAAEYKGKDRHVIYGRCAHLTAP
                     QPIHLQQGRGKCQDRNLCQRGCPFGGYFSSNSSTIPWAERSGHLTLRPFSVVHSILYD
                     PAKGLAKGVRVIDARTKEVFEYFAPVIFVNASAMNTNLILLNSVSARFPHGLGNDNGL
                     LGKFVAFHNYRGSIWGEHPGFKDSTTDGRTPTNAYMPRFRNLRRQETDFLRGYASTVS
                     GWRNTNRDYTGTGAALKKGLLNTSLGPWSMGCQMMGETIPRETNQLTLDPEKKDEWGM
                     PLLRFNIGYTDNEEKMLKDFFAEMSEMMTKAGFTNIQTRDSGQAPGLDIHEMGGVRMG
                     HDPKTSLLNRWNQLHACKNVFVTDGACMTSTSTQNPSLTYMAITARAANYAIGALKRR
                     SWEG"
     gene            complement(2221..2760)
                     /locus_tag="VKU83_07395"
     CDS             complement(2221..2760)
                     /locus_tag="VKU83_07395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLI93416.1"
                     /translation="MTITRRTALQRMFIVAGGVVLLPACGRNNDNHDENQETLEALAD
                     TLLPAGSTPADQTLGAKAAGAHLFAARMIRDCYPKAQQEQYMRGLEAFNQQARSKYGK
                     PFAECNQGQRDALVSAINASTGQKTDDLSFFFRENKNLVMQGYLGSQYFLTKIEVYEL
                     VPGRWHGCTPVVKQSHKQS"
BASE COUNT          597 a          748 c          846 g          707 t
ORIGIN      
        1 cgataaggac ggcaacgtaa agaactggtg gacgaaggaa gattatgaga agttccgggc
       61 aaagacgaaa cgggtcattg acttgtacag cggttttacg gtcttggata cggtgcatgt
      121 caaaggcgcg ctgacagtag gtgaaaatac cgcagataac ggcggggttg cgattgccta
      181 cgacgcattt aaaatgacgc cggaagggaa ggattccagt atccggattg atggttttag
      241 tcccgatcag cggttctttc tttccattgc ccgcatctgg aggctcaaaa caagagatgc
      301 ttatttgcgc atgtatgtga ataccaaccc gcattcgccg gcgatgtggc gcgtaaatgg
      361 tccgctgatg agccgcgagg cgttttacaa agcatttaat gtgcagcccg gcgacaaaaa
      421 ttatctgcca gatgatcagc gaataaagat ctggtaggca tccctggtcg ccgattgccg
      481 ggccgaccgt agttggggca gttccttagc cttcccagct cctgcgtttc aaagcgccta
      541 ttgcatagtt cgcggctctg gcggtaatcg ccatgtaagt gagcgacggg ttttgcgtgg
      601 acgtggaggt catacaggcc ccatccgtga caaacacatt tttgcaggca tgcagttgat
      661 tccacctatt gagcagggag gtctttggat cgtgacccat gcgaacgccg cccatttcat
      721 ggatgtcaag gcccggcgcc tggccggaat cccttgtctg gatgttggtg aatccggctt
      781 tggtcatcat ctccgacatt tcagcgaaga aatctttgag catcttttct tcattgtcgg
      841 tgtagccgat attgaaccgc aataagggca tgccccattc gtccttcttt tccggatcga
      901 gggtaagctg gttcgtttcc cgcggtatgg tctcgcccat catttggcaa cccatactcc
      961 agggaccgag gctggtgttt agcagcccct tcttcagcgc cgcgccggta ccggtatagt
     1021 cccggttggt gttccgccag ccggagaccg tgcttgcgta acctctcagg aagtctgtct
     1081 cctggcggcg aaggtttcta aaccgcggca tataggcatt tgtaggtgtc cggccgtctg
     1141 tcgtgctgtc tttaaatccg gggtgttcgc cccagatgct gccccggtaa ttatgaaagg
     1201 caacgaactt gccgaggagt ccattgtcgt tgccgagtcc gtgcggaaag cgggccgaaa
     1261 cggagttcag caagatcagg ttggtgttca tggcgcttgc gttgacgaag atcactggcg
     1321 caaaatattc gaacacctct ttagtgcggg catcaatgac ccgtactccc tttgccagcc
     1381 ctttggcggg gtcgtagagg atggaatgaa cgacagaaaa agggcggagc gtcagatgac
     1441 cgctgcgttc ggcccagggt atggtagagg agttacttga aaaataaccg ccgaaggggc
     1501 agccccgctg gcaaaggttc cggtcctggc atttgcccct gccctgctgc agatggatag
     1561 gctgcggagc cgtgagatgg gcgcagcggc cgtagatgac atggcggtct tttcccttgt
     1621 actcggccgc tacgacctgg cggaaatgat cttcgatgca cgtgaggtcc agcggcggca
     1681 gaaattcgcc atccggcagt tggtccaggc cgtcgcgatt acccgagatg ccggcgaatt
     1741 tctcaacgtg gctataccag ggcgcgaggt ctttataccg gatcggccag tctaccgcat
     1801 agccgtcccg cgcaggtcct tcaaagtcaa agtcgctcca acgctgcgtc tgccgcgccc
     1861 acattaagga tttgccgccc acctggtagc cgcggatcca gtcaaaaggc ttttgctgga
     1921 cgtaaggatg ctccgtatct tttacaaaga agtgcattgc gtcctcctga aaggcatagc
     1981 accggctgat cgacggattg gcctgctgga tgtccagcgg cacctgaccc tggtgcggaa
     2041 attcccaggg cattttattt gtcgtcggat agtctttgag atgttttaca tcccgccctc
     2101 gttccagtac gagggtcttc agaccgctat cggtaaattc cttggcagcc cagccgcctg
     2161 acatgccgga gccgatgaca atggcgtcga atgtattcct ggattttgag tctcccataa
     2221 ttagctctgt ttgtggctct gcttgacgac cggtgtacac ccgtgccagc ggccaggcac
     2281 caactcgtac acttcaattt tggtgaggaa atattgtgaa ccgagataac cctgcatcac
     2341 gaggttctta ttttcgcgaa agaaaaagga aaggtcgtcg gtcttttggc cggtggaggc
     2401 gttgatggcc gatacgaggg cgtctctttg cccctggttg cattcggcaa aaggctttcc
     2461 gtatttgctg cgggcctgct ggttgaaggc ttctaggccg cgcatatatt gctcttgctg
     2521 ggcttttgga taacagtcgc ggatcattct tgctgcaaac aggtgagcgc cggcggcttt
     2581 tgcgccgagg gtttggtcgg ccggcgtaga gcccgcaggc aagagggtat ctgcgagggc
     2641 ttccagggtt tcctggttct catcgtgatt gtcgttgttc cggccacagg cgggcaaaag
     2701 gactacgccg ccggcaacga tgaacattcg ctgaagggcg gtgcgtcggg taattgtcat
     2761 gcaaatcgct tttgaagctc ctaaaatacc cttttttggg attatgacca accgccgacg
     2821 cgatttcccg tattactctg aacggagact tttcaccggg ttggtcaagg cggctttaag
     2881 ggtatgcgca ctgatcgc
//
LOCUS       DATGKE010000428         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1475727, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000428 DATGKE010000000
VERSION     DATGKE010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475727"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="recF"
                     /locus_tag="VKU83_07400"
     CDS             <1..844
                     /gene="recF"
                     /locus_tag="VKU83_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HLI93417.1"
                     /translation="TVEVVCILRETGKKEFLASGQAYEKLSHHIGRFPCVIIAPDDIQ
                     MVTGGSEERRRFLDAVLSQADPGYLQNLIDYNRILQQRNGFLKSLAGRRLIDRGLLET
                     YDGQLVRRGNQIFERRRQFLQEFLILAGKHYTEVAGRDEPVALAYESQLLYASFADLL
                     RQGLDRDIDAQRTSCGIHKDDIGLLYADLPFKMMASQGQRKSLLFALKLAEYETLRQA
                     KGFWPLLLLDDAFEKLDEGRMRNLLDRVAKDGGGQVFITDTHEERMRAQLDRLSVPYQ
                     IVLL"
     gene            954..1400
                     /locus_tag="VKU83_07405"
     CDS             954..1400
                     /locus_tag="VKU83_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93418.1"
                     /translation="MPSDLETSIRLIEETNSPEKAYFSYSVDDSGEYGVIKANKEGLR
                     LYAADLLKKSLQMEEDAGGKQPLFFSPKDWMYSETGYNLIAGVLPEYEPRENILLRGL
                     RPVRPLRPRMRRTQVAYFVLMCITGAVLMLVSLKAFPNLRLWVNIR"
     gene            1382..1663
                     /locus_tag="VKU83_07410"
     CDS             1382..1663
                     /locus_tag="VKU83_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HLI93419.1"
                     /translation="MGEHSLGDALRKFLHQSQLKGSIQALQIEDAWERIMGKTVARYT
                     DKIRIHGQTLYIETGVAPLRQELLYQKEAIVKRVNEALGETVIKEVVIK"
     gene            complement(1746..>2738)
                     /locus_tag="VKU83_07415"
     CDS             complement(1746..>2738)
                     /locus_tag="VKU83_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93420.1"
                     /translation="VVAVFFLFQGFGDPTRLLIGQRADAATQDNIRKELYLDQPRWKQ
                     FIYYLDDISPVAIHTREGISQKGLKGLFIGGRTCLVFKWPYLRRSYQSKREVWDILME
                     ALPGTILLAMAAMIFATIVGIGLGVLAAIRKDTWMDTSAIFLSVVGISAPSFFMGIVI
                     AYLFGFVLSGWTGLHMTGSLIDTDPFTGRHLELRNLVLPALTLGIRPLAIIAQLTRGA
                     LLDVLDQDYIRTAYAKGLPARTVIWKHGLRNALNPVITAITGWFAELLAGAFFVEEIF
                     GWKGIGKVTVDALENLDFPVVMGSVLVTSFFFIVVGLLSDLLYGWIDPRVRLTR"
BASE COUNT          682 a          719 c          779 g          558 t
ORIGIN      
        1 gaccgtggag gtcgtttgca tcctccggga aacggggaag aaagaatttc tggcgagtgg
       61 tcaggcctat gaaaaactca gccaccatat cgggcgattt ccctgcgtta tcatcgcgcc
      121 cgacgatatc caaatggtca ccggcggaag cgaagaacgg cgaaggtttc tcgatgcggt
      181 gctgtcacag gcggacccgg gctacttaca aaacctgatc gactacaacc ggatcctgca
      241 gcaacgtaac ggctttttga aatcccttgc cggtagaaga ttgattgata gaggcttgct
      301 ggagacctat gacgggcagc tggttcggcg cggaaaccag atcttcgagc ggcgccgcca
      361 gttcctgcag gagttcctta tcctggcagg aaaacattat acggaggttg ctggacgcga
      421 cgagccggtt gcgctggctt atgaaagcca gctcctttac gcttctttcg cggacctttt
      481 gcgccagggc ctggatcggg atatagacgc tcagcgcacc tcctgtggaa ttcacaagga
      541 cgacatcggc ctgttgtacg cggatctccc gttcaaaatg atggcttcgc agggccaacg
      601 gaagagcctg ttgtttgcgt taaaactggc agagtacgag acgctccggc aggcaaaggg
      661 tttttggccc ctgcttttgc ttgatgacgc gttcgagaag ctggacgaag gcagaatgcg
      721 caatctgctc gaccgggtag ccaaagacgg aggtggccag gtctttatca ccgatacgca
      781 tgaggaacgg atgagggcgc agttggacag gctttcggtg ccttatcaaa tcgtcttgct
      841 ttgatcggct aaaaccagaa atttattagc gcctttgttg gagttttggg tgaaattccg
      901 ttaaatttac tgacacacac atcaaaaaat aaagtccaga aaatctgatt gccatgccgt
      961 ccgacctaga gacttctatt cgacttatag aagaaacaaa cagccctgag aaagcatatt
     1021 tttcctattc agtagatgac agtggagaat atggcgtcat caaagcaaac aaagaagggc
     1081 tccggcttta tgccgccgac ctgttgaaaa agtccctcca aatggaggag gacgcgggcg
     1141 gaaagcagcc gctttttttc agccctaaag actggatgta cagcgaaacc ggctataacc
     1201 tgatcgccgg tgtcctgccg gagtacgaac caagggaaaa tatcctgttg cgtgggcttc
     1261 gtccggtccg gccgctgagg ccgcggatgc gcaggaccca ggtcgcttat ttcgtgctca
     1321 tgtgcataac cggcgccgtt ctgatgctgg tttccctaaa agcttttcct aacttgcgcc
     1381 tatgggtgaa cattcgttag gcgacgcatt acggaaattc cttcatcaaa gtcagcttaa
     1441 aggatcgatc caggcgctgc agatcgaaga cgcctgggaa aggatcatgg gtaagacggt
     1501 cgcgcggtat accgacaaga tccgtatcca tggccaaacg ctctatatcg aaaccggagt
     1561 ggctcccctg cggcaggaac tgttatatca gaaagaggcg attgtcaaac gggtgaatga
     1621 ggcgctggga gaaaccgtga ttaaagaggt ggttatcaag taggggctgg gcgccgccta
     1681 cgctaaaact cccgctggac aagcggccac aaaagctcgc tttcgctgag gcggcagcgg
     1741 cgaagtcagc gggtgagccg cacgcgggga tcgatccagc cgtacaacag gtccgacagc
     1801 aagccgacaa cgataaaaaa gaacgacgtc accagcaccg atcccatgac gaccgggaaa
     1861 tccaggttct ccagggcatc cacggtgact ttgccgatcc ccttccagcc aaaaatctct
     1921 tcgacaaaga aagcgcccgc aagcagttcg gcgaaccagc ccgtaatggc ggtgatcacc
     1981 gggttcagcg cgttccggag cccgtgtttc cagattaccg tcctggcggg caggcccttc
     2041 gcataggccg tccggatgta gtcctgatca agaacatcca acagcgcgcc acgggtcagt
     2101 tgggcgatga tcgccagcgg cctgatccca agcgtcaatg ccggcaggac caggttacgc
     2161 aattcgagat gtcgtccggt aaaaggatcg gtgtcgatca ggctccctgt catgtgcagc
     2221 ccggtccagc cgctgagcac gaaaccgaac aaataggcaa tgacaatacc cataaagaag
     2281 gagggggcgg agatcccaac gacactcagg aaaatggcgg aggtgtccat ccaggtgtct
     2341 ttgcggatgg cagccagtac tccgaggccg atgccaacaa tagtcgcaaa gatcatggct
     2401 gccatggcca gcagaatggt gccggggaga gcttccatca ggatgtccca gacttcacgc
     2461 ttggattggt aggaccttcg caggtaaggc catttaaaga caaggcaggt cctgccgcca
     2521 ataaagagac ccttcagccc cttttggctg atcccttcac gggtgtgaat ggcgaccggc
     2581 gagatatcgt cgagataata gatgaactgc ttccagcggg gttggtcgag gtaaagttcc
     2641 tttctgatat tatcctgggt ggccgcatcc gcacgctgcc cgatcagcag ccgcgtcgga
     2701 tcgccaaaac cctgaaaaag gaaaaatacc gccacgac
//
LOCUS       DATGKE010000429         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1352272, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000429 DATGKE010000000
VERSION     DATGKE010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352272"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="murI"
                     /locus_tag="VKU83_07420"
     CDS             complement(<1..658)
                     /gene="murI"
                     /locus_tag="VKU83_07420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLI93421.1"
                     /translation="MEPLQPIGVFDSGYGGLTVLRELIARLPQYDYVYLGDNGRAPYG
                     NRSFETVYQYTLQSVEWLFHRGCPLVILACNTASAKALRTIQQVDLPKIAPDNRVLGV
                     IRPTTEVIGNYTHTGNVGILATRGTVLSGSYPIEIGKFYPGLRVYQQACPLWVPLIEN
                     NEHDKPGADYFVREYMDQLRAQSAAIDTVLLACTHYPLLAEKIAACLPAGARLVAQGE
                     I"
     gene            complement(757..1266)
                     /locus_tag="VKU83_07425"
     CDS             complement(757..1266)
                     /locus_tag="VKU83_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLI93422.1"
                     /translation="MKKFVTVLAVCASLVLAGSSTQAQGKIGVISLAELVPNMPEYKK
                     ADTAMNEFNNALQQQYQEMVNEYQTQAAELSSKDTAKYTSAQLEIKRKDLGDLLTKLQ
                     GWNQQAQQMQNQKNQELMAPITKKAMEAIQAVAKENGYTYILSKDQALVSPPGDDILP
                     LVKKKLGIK"
     gene            complement(1310..1822)
                     /locus_tag="VKU83_07430"
     CDS             complement(1310..1822)
                     /locus_tag="VKU83_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLI93423.1"
                     /translation="MKKLALSGAFLLMIAVAAQAQSYAVVDTKYILEHMPEYKDAQKR
                     LDATSAMWQKEIDDKQAALNKMYKDYDAEKVMLSDTLQKKREIELFNTEKEVRDLQRK
                     RFGYEGDLFKLRQELVKPIQDKVYNAIQQLAVSRMYDFILDKSEGITVIFADPKLDKS
                     EDVLRILGIK"
     gene            complement(1857..>2402)
                     /locus_tag="VKU83_07435"
     CDS             complement(1857..>2402)
                     /locus_tag="VKU83_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLI93424.1"
                     /translation="YLPIGNATGADKSRQFVVKVSAKYGFMGRYNPALGFSPFERFQL
                     GDAGLTNNFGLLGYDIISQRGYPVYQNSNPKVNPDQQQATQFFVLFNKYTLELRYPFT
                     TNPNSTIYGLAFFEAANGWYDYTTYNPFQLRRSVGLGMRFFLPMFGLIGFDYGLGLDR
                     IQNGSIKDATRFTFLLGYEPD"
BASE COUNT          518 a          660 c          612 g          612 t
ORIGIN      
        1 cgatctcccc ctgcgcgaca aggcgggcgc cggccggcag acaggccgca atcttttccg
       61 ccaataacgg atagtgggtg caggccagca ataccgtatc gatagccgcg ctttgggccc
      121 gcaactgatc catgtactcc cggacaaagt agtccgcccc gggcttgtca tgttcgttgt
      181 tttcgatcag tggcacccat aatggacagg cctgctggta aacccgcagg ccgggataaa
      241 atttcccaat ttcgatcggg taggaaccgg acaggaccgt gccccgggtc gccaatatcc
      301 ctacgttgcc ggtatgcgta tagttgccta tcacttcggt agtgggccgg atgactccca
      361 gcacccggtt gtccggcgct attttcggca ggtccacctg ctggatggtg cggagcgcct
      421 tggcggaagc ggtattgcag gcgagtatca ccaatgggca gccgcgatgg aaaagccatt
      481 ccaccgattg gagggtgtac tgataaacag tttcgaacga gcggttcccg taaggcgccc
      541 gtccattgtc cccgaggtag acatagtcat attgcgggag cctggcgatc agctccctga
      601 gcacggtgag cccgccgtat cccgaatcga atacgcctat cggttgtaat ggttccatgc
      661 ggcgaaatta acaaataaaa aggggtggca aggccggggc ggcgcgcaat aaaaacgccg
      721 gagcagaacc ccggcgcttc atcaaatgaa ttcccgttat tttatcccca gcttcttctt
      781 taccagggga agaatatcgt ccccgggcgg cgataccagc gcctgatcct tcgaaaggat
      841 ataggtatag ccgttctcct tggcgacggc ctggatcgct tccatggcct tctttgtgat
      901 cggcgccatt aactcctggt tcttctggtt ctgcatctgt tgcgcctgct ggttccaacc
      961 ctgcagtttc gtcagcaggt caccgaggtc cttgcgcttg atctcgagct gtgccgacgt
     1021 atatttcgct gtatccttag agctcaactc ggcggcctgg gtctggtact cattcaccat
     1081 ctcctggtat tgctgttgca gcgcgttgtt aaattcgttc attgccgtgt ctgccttctt
     1141 gtattccggc atgttcggga cgagctcggc caggctgatg acgccgatct taccctgggc
     1201 ctgtgtcgaa ctgcccgcca ataccaggct cgcacacacc gcgaggaccg taacaaactt
     1261 tttcattgtc gtttttatag tttttagttg ttttgcttat atgacgattt tacttaatac
     1321 ccaaaatcct cagaacatct tcgctcttgt ccagcttggg gtcggcaaag ataacagtaa
     1381 ttccctcact tttgtccaaa ataaaatcgt acatcctgct aaccgcaagt tgttgaatcg
     1441 cattatagac tttgtcctgt atgggtttga ccagctcctg gcgcagttta aaaaggtctc
     1501 cctcataacc gaagcgcttg cgttgcaggt cccttacctc cttctccgtg ttaaataatt
     1561 cgatctcccg ttttttctgt aacgtatcgc tcagcatcac cttttcagcg tcatagtcct
     1621 tatacatctt gttcaatgcg gcctgcttgt cgtcgatctc cttctgccac atcgccgatg
     1681 tcgcgtccag cctcttttgc gcgtccttgt attcaggcat atgctccagg atatatttcg
     1741 tatcgacaac ggcatagctt tgtgcctggg cggcaacagc gatcatcagc aaaaaggctc
     1801 cggaaagcgc taactttttc atggtcagac aaattttaga catgaattta atcattttaa
     1861 tccggctcgt atcctaacag gaatgtaaag cgcgtcgcat ccttgatcga cccgttttga
     1921 atccggtcca gaccgagtcc gtagtcgaag ccgattaacc cgaacatcgg caggaagaac
     1981 cgcatgccca gaccgacgct ccggcgaagc tggaaaggat tgtatgtcgt gtaatcatac
     2041 catccgttcg ctgcctcaaa gaaagccagg ccgtaaatcg tgctgttggg gttggtggtg
     2101 aagggatacc gcaactccag ggtatattta ttgaacagga cgaaaaattg cgtggcctgt
     2161 tgctggtcgg ggttcacttt gggattggaa ttctggtaga ccggatagcc gcgctgggaa
     2221 ataatatcat aacccagcag gccaaagtta ttcgtcagac ccgcatcgcc gagctggaac
     2281 cgctcaaaag gcgaaaaccc gagcgccgga ttatagcggc ccatgaatcc gtatttagcc
     2341 gaaaccttca cgacgaactg cctgcttttg tcggccccgg tcgcattgcc gatcggcagg
     2401 ta
//
LOCUS       DATGKE010000430         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1343687, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000430 DATGKE010000000
VERSION     DATGKE010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343687"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="glyA"
                     /locus_tag="VKU83_07440"
     CDS             <1..919
                     /gene="glyA"
                     /locus_tag="VKU83_07440"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLI93425.1"
                     /translation="HGSPVNFSGKLYRPVFYGVKKEDGRVDYSMMEQVAKKEKPKLII
                     CGASAYSRDWDYKRIRAIADSVGAFVMCDMAHPAGLIAKGLLTAPFEDCHFVTSTTHK
                     TLRGPRGGIILMKKDFDNPFGLKDVKGNIRPMSNLIDMAVFPGTQGGPLEHVIAAKAV
                     AFGEILSAGFSAYAKQIIANAQAMSAVFAAKGYHIVSGGTDNHLLLIDLRNKNISGKK
                     AEIALGKADITVNKNMVPFDDKSAFVTSGIRVGVPAITTRGMKEGHMATIVEMIDKAI
                     LNAENEAALHSLKGDVNALMKQFPLYPEL"
     gene            931..1380
                     /locus_tag="VKU83_07445"
     CDS             931..1380
                     /locus_tag="VKU83_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HLI93426.1"
                     /translation="MLQRKQTLWMILAVICEVLTFRFPFYQGNVTVGNYGHELRGLTA
                     WPHYVNGASGSILITLITTILIVGTIWDIGSYKNRRGQFWITLGLIVLSLLNILAYWI
                     HSGKPDFLEGGFSLTAVFALAIPFFLILAARGIRKDEKLVKSADRLR"
     gene            1486..>3479
                     /locus_tag="VKU83_07450"
     CDS             1486..>3479
                     /locus_tag="VKU83_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93427.1"
                     /translation="MLKNYLRVAFRNLWRHWGFSLLNVLGLTIGMSACFLIFLYVKFE
                     LSYDHFHAKADRIYRVVTDIVNPSETLNLDVAAPAMPVAAKRDFPEIGKEVRFDPDNL
                     LVRRGEIKIQETHMAYADSTFFEVFDFPLLKGDPATALRESGSMVLSETAAKKYFGSA
                     DPMGQHLYMGEDNKNIGTVTGVMKDMPENTLLKADLIVSRYSNQKRDSAVDQNWGGFG
                     DYTYFLLKPHTDPKALERKFPPFLVNHIGKMMKDINQTYIYRLEPLKDVYLYSNRNDA
                     KAAHITNVYVFSIIGVFILLIAGINFVNLTTARSTERAREVGIRKVIGAERGQLTGQF
                     LGESVILCLIAFVLSVAACAALIPSFNFLAGKTVSTGILHHPGYIGALLLIGLGIGLL
                     AGIYPALVLSAFQPIIVLKGRFATGARGILLRKGLVVSQFTISIGLIAATLLVGYQLN
                     YMRSQQLGFSKDQELVLDTHGDEHRVAVADEIRRLPGVLSTALSSHTPGMGAMNAYST
                     IQNQKGDMQICSPDLFFVDFDFIPQYQMKLVAGRTFSRAFGTDTTQAMVLNEEAVRML
                     GYHSPQDVIGRNFDQWGRKGKIIGVVQDFHYQGLQQAIRPLSMRIEPGGCNIISVKVR
                     ASNLKGTIASIENIWRTMIPNEPFSYSFVDETFDRQYRAE"
BASE COUNT          748 a          968 c         1009 g          754 t
ORIGIN      
        1 gcacggctcg ccggtaaatt tttccggcaa gctttaccgg cccgtatttt atggagtaaa
       61 aaaggaagac ggcagggtcg actattccat gatggagcag gtcgcaaaaa aagaaaagcc
      121 gaagctcatc atttgcgggg cttcggcgta tagccgtgac tgggattaca aacgcatccg
      181 ggcgatcgcc gacagtgtcg gcgcctttgt gatgtgcgat atggcgcacc ccgcgggttt
      241 gatcgcaaag gggctgctga ccgcgccgtt tgaggactgt catttcgtaa cctcgacgac
      301 gcataagacc ttgcgcgggc cgcggggagg cattattctc atgaagaaag actttgacaa
      361 cccgttcggc ttgaaagacg tgaagggaaa tatccgtccg atgagcaacc tgatcgatat
      421 ggccgttttt cccggtaccc agggcggtcc cctggaacac gtgatcgcgg ccaaggcggt
      481 ggcgtttggc gagatccttt cggctggttt tagcgcctat gcgaagcaaa ttatcgccaa
      541 tgcgcaggcc atgtcggccg tctttgcggc taaaggatat cacatcgtat ccggcggcac
      601 ggacaaccac ctgctgctga tcgatctccg caacaagaac atcagtggga aaaaggccga
      661 gatcgcgctc ggcaaagcgg atatcaccgt caataagaac atggtgccct ttgacgacaa
      721 gagtgcgttt gtcacgtccg gcatccgggt cggggttccg gccattacaa cgcgcggaat
      781 gaaggaaggc catatggcca ccatcgtgga aatgatcgac aaggcgatcc tgaatgcgga
      841 aaacgaagca gcgctgcact cgttgaaggg cgatgtcaat gccttgatga agcagttccc
      901 gctgtacccc gaactttaaa attccttttt atgttacagc gcaagcagac cctttggatg
      961 atactggccg ttatttgcga agtgctgact ttccggtttc cattctatca gggaaatgtc
     1021 accgtgggca attatggcca cgagctccgg ggattgacgg cgtggcccca ctatgtcaac
     1081 ggcgccagcg gcagcatcct gatcacgttg atcacgacca tcctcatcgt cgggaccatc
     1141 tgggacatcg gcagctacaa gaaccggcgc gggcagtttt ggatcaccct gggacttatc
     1201 gtcctttcgc tgctgaatat cctcgcgtac tggatccata gcggcaagcc cgactttctc
     1261 gagggcgggt tcagtcttac tgccgttttt gcgctggcca tccccttttt cctgatcctg
     1321 gcggcgagag ggatccgcaa ggatgagaag ctggtgaaga gcgccgatcg cctgaggtag
     1381 ccgctcaccg gagccggccg ctcgccggag ccagccgctc gccagagcca ggcaacgccc
     1441 ggtcgcccgg atgcaacatt tttcccggaa ccccgtcatt ccctcatgct aaagaattat
     1501 ctgcgcgtag ccttccgcaa tctgtggcgg cactggggat tctccctttt aaacgttttg
     1561 ggcctcacga tcggcatgtc ggcctgcttc ctgatcttct tatatgtgaa gtttgagctc
     1621 agctacgacc acttccatgc gaaggccgac cgcatctacc gggtcgtgac ggacatcgtc
     1681 aatccttcgg agacgctgaa tttagacgtt gccgcgccgg caatgccggt ggctgcgaaa
     1741 cgggactttc ccgagatcgg gaaggaggtg cgctttgatc cggacaacct cctggtcagg
     1801 aggggagaga tcaagatcca ggagacccat atggcctacg cggattcgac gttcttcgag
     1861 gtcttcgatt ttccactgct taagggcgat ccggccacgg cgttgcggga atccggtagt
     1921 atggtcctct cggaaaccgc cgcaaagaaa tatttcggtt ccgccgatcc catggggcag
     1981 catttgtata tgggcgaaga caacaagaac atcgggacgg ttacgggagt catgaaggat
     2041 atgccggaga acaccctgct gaaggcggat ttgatcgtgt cgcggtattc gaatcaaaag
     2101 cgcgactcag ccgtggacca gaattggggc gggttcggcg attacactta cttcctcctg
     2161 aaaccacaca cggatccgaa ggcgctcgaa aggaaattcc ccccgttcct ggtcaatcat
     2221 atcgggaaaa tgatgaagga tatcaaccaa acctatattt atcgcctgga accgctgaag
     2281 gacgtgtacc tgtactccaa ccggaacgac gccaaagccg cccatatcac caatgtttat
     2341 gtcttttcca tcatcggcgt ttttatcctc ctgatcgcag gcatcaattt cgtgaacctt
     2401 acgacggcgc ggtcgaccga aagagcccgc gaggtgggga tccggaaagt gatcggcgcg
     2461 gagcgggggc agctgacggg ccagttcctc ggcgaatcgg ttatcctgtg cctgatcgcg
     2521 tttgtgttgt ccgtcgcggc ctgcgccgcg ctgatcccca gcttcaactt cctggcgggg
     2581 aagacggtca gcaccgggat cctgcatcac cctggttata tcggcgcgct gctcctgatc
     2641 ggcttgggca tcgggttact cgcgggtatc tatccggcgc tggtcctttc ggctttccag
     2701 ccgatcatcg tgctgaaagg gcgctttgcc accggcgccc gggggattct tctccggaaa
     2761 gggcttgtcg tctcgcaatt cacgatttcc atcggcctga tcgccgctac cttgctcgtc
     2821 ggctaccaac tgaattatat gcggagccag cagctcgggt tcagcaagga tcaggagctc
     2881 gtgctggaca cccacggaga cgagcaccgg gtggcggtag cggacgagat ccggagattg
     2941 cccggtgtgc tctccaccgc cctgtcctcg catacgccgg gcatgggagc aatgaacgcc
     3001 tattccacta tccagaatca gaagggcgac atgcagatct gcagccccga tctctttttt
     3061 gtcgactttg actttatccc gcaataccag atgaagctgg tggcggggcg gaccttttcc
     3121 cgcgcgttcg gcacggatac cacgcaagcg atggtgctga acgaggaagc ggtgcggatg
     3181 ctggggtatc actcgccgca ggatgtgatc ggcaggaatt tcgaccagtg gggcaggaag
     3241 ggcaagatca tcggggtagt gcaggatttc cattaccagg gtctgcagca ggcgatcagg
     3301 ccgctcagca tgcggatcga acccggtggc tgtaatatca tctctgtaaa ggtccgcgct
     3361 tccaacctga aaggaacgat cgcttcgatc gaaaacatct ggcggaccat gatccccaac
     3421 gagcccttca gctattcatt cgtagatgaa acgtttgacc ggcaataccg cgcggaaga
//
LOCUS       DATGKE010000431         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_122580, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000431 DATGKE010000000
VERSION     DATGKE010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122580"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VKU83_07455"
     CDS             <1..225
                     /locus_tag="VKU83_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93428.1"
                     /translation="ALPPRKLGEMIREARNYSFDFLGWKKEFTLRQHWLVHTKKICPR
                     CRIPVIKVPRLGKAGRRAFYCGNCQLLYRG"
     gene            complement(239..1258)
                     /gene="glk"
                     /locus_tag="VKU83_07460"
     CDS             complement(239..1258)
                     /gene="glk"
                     /locus_tag="VKU83_07460"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HLI93429.1"
                     /translation="MIPIAFSNESLPHQGAVAILAADIGGTKTNVALYTADSNGLAVT
                     REKRYASGDHASLTDIVRDFCGKNLPDRISAAVAGPVIGGKSRLTNLPWILDSAAMSR
                     ELGIPVRFINDLEATAYGLAGLKAEECATLATGDPAAKGNIAILAPGTGLGEAGLYWD
                     GARYHPFATEGGHSDFAPRTEQDVGIFYFLQRQYGHVSWERVVSGMGIKNIFQFLTGA
                     QKKQIPDWLANRLQTEDPAAVISETALRRADPVCIETMEIFVRYLATEGASLVLKLMA
                     TGGLYLGGGIPPKILPLLQTDTWKKNFDNNGRMHELSDRIPVNVVLNDKMALQGAAYY
                     GAFNM"
     gene            complement(1268..>2095)
                     /locus_tag="VKU83_07465"
     CDS             complement(1268..>2095)
                     /locus_tag="VKU83_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLI93430.1"
                     /translation="NLSFFFSNGMDPEYSVIGRVARRIWAKAIKQLYKGNSRSQKLKY
                     HIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAFDEAITTPTEASVRRAMA
                     IQLIINRELGSAKTENFTQGSFAVEELTDLVEEAVLAEFDRLTDRGGVLGAMERMYQR
                     NKIQEESLYYESMKHSGALPIVGVNTFLGREGSPTILPDEVIRSTSGEKDQQIRNLEA
                     FHSRHADRRPEMLRRLQEVAVTNGNLFDELMKTVKYCSLGEITNALYEVGGQYRRNM"
BASE COUNT          369 a          607 c          630 g          489 t
ORIGIN      
        1 gccttgccgc cgcggaaact cggggagatg atccgcgagg ccaggaatta cagtttcgat
       61 ttcctcgggt ggaaaaagga gtttacgctt cggcagcact ggctggtcca tacgaagaag
      121 atctgtcccc gatgccgtat ccccgtgatc aaggtgccgc gcctcggaaa agccggccgc
      181 cgggcatttt actgcggcaa ctgccagctg ttgtaccgcg ggtaacccgg gcgaaccgct
      241 acatgttgaa ggcgccgtag tacgccgcgc cctgcagggc cattttgtcg ttcagcacta
      301 cgttaaccgg gatgcggtct gacaattcgt gcattcgacc gttgttgtcg aaattttttt
      361 tccaggtgtc ggtctggagc agcggcagga tcttcggcgg aattcctccg ccgaggtaaa
      421 ggccgccggt agccatgagc ttcaacacga ggcttgcgcc ttcggtcgcc aggtaacgga
      481 cgaagatctc catggtttcg atgcataccg gatccgcgcg gcggagggca gtctcgctga
      541 tcacggcggc gggatcttcg gtttgcaggc gattcgccag ccaatccggg atttgttttt
      601 tttgggcgcc ggtcaggaat tgaaagatat tcttaatacc catgcccgac acaacccgtt
      661 cccagctgac gtggccatac tgtctttgca ggaaataaaa tatcccgacg tcctgttccg
      721 tgcgcggcgc gaaatcgctg tggccgcctt ccgtcgcaaa tggatggtac cgcgcgccat
      781 cccagtacag ccctgcttcg ccgagtccgg ttcccggagc aaggatggcg atattgcctt
      841 tcgcggccgg gtcccccgtg gccagcgtcg cgcattcctc ggctttaagc ccggcgagac
      901 cgtaggcggt cgcctccagg tcattgataa aacgaacggg aatgcccagc tcccggctca
      961 tggctgcgct gtccaggatc cacggcaggt tggtgagcct gcttttcccg ccgatcaccg
     1021 gaccggcgac tgcggcggag atccggtccg gcagattttt tccgcaaaaa tcccggacga
     1081 tatcggttag cgatgcatga tccccggagg cgtatcgctt ttcgcgcgtg acggcgagac
     1141 cgttgctgtc ggcggtatat agcgccacgt ttgtttttgt tccgccaata tcggccgcca
     1201 ggatggcgac cgccccttga tggggaaggg actcgttgct gaaggcgatg ggtatcatcg
     1261 gccaaaatta catgttgcgg cgatactggc ccccaacttc gtagagggca ttggttattt
     1321 cgccgagact gcaatacttc accgttttca tgagctcatc gaaaagattg ccgttggtaa
     1381 ccgccacttc ctgcaggcgg cgcaacattt ccggccggcg gtcggcatgg cggctgtgga
     1441 aggcctcgag gttgcggatc tgctggtcct tttccccgct cgtgctgcgg atcacctcat
     1501 ccggtaaaat ggtcggcgag ccttccctgc ccaggaaggt gttgacgccc acgatcggca
     1561 aggccccgct gtgcttcatg ctttcgtaat acaggctctc ttcctggatc ttgtttcgct
     1621 ggtacatcct ttccatggcg cccagaacgc cgccgcgatc ggtcagccgg tcgaactcgg
     1681 cgagcaccgc ttcctccacg aggtccgtga gttcttcgac ggcgaagctc ccctgcgtaa
     1741 aattctccgt tttcgcactg cccagttccc ggttgatgat gagctggatg gccatggcgc
     1801 ggcggacgct ggcctccgtc ggcgtcgtaa tggcttcgtc aaaggcattt gtgtgcagcg
     1861 agttgcaatt gtcgtagatc gcatagaggg cctgcagcgt ggtgcggata tcgttgaaat
     1921 cgatttcctg ggcatgcagg ctccggccgc ttgtctggat gtggtacttg agtttttgcg
     1981 agcggctgtt gcccttatag agttgtttga ttgccttggc ccagatcctt ctggccacgc
     2041 gaccgatgac gctgtattcc gggtccatgc cgttgctgaa gaagaacgac aggtt
//
LOCUS       DATGKE010000432         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1255077, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000432 DATGKE010000000
VERSION     DATGKE010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255077"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VKU83_07470"
     CDS             complement(<1..916)
                     /locus_tag="VKU83_07470"
                     /inference="COORDINATES: protein motif:HMM:NF001133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HLI93431.1"
                     /translation="MALLHNRVSQKELKQRLLEEKEQRITLSFYRYVPIADPAAFRDM
                     LYVELHVLAVFGRIYVAAEGINAQVSVPASRLEQLKSRLESIPGLEALRLNRAFDDDG
                     KSFWVLKIKVRKKIVADGIHDPSFDMSRKGKYVDAAAFNQLAADPATVIVDMRNHYEF
                     EVGHFDRAIQIPSDTFREQLPMSAELLKDKKDSPIIMYCTGGIRCEKASAWLLHKGFS
                     KVYHLEGGIIHYANTVKEKGLDNKFRGKNFVFDDRLGERISAEVIAACHQCGRPADTH
                     TNCRNDACHLLFIQCNECAAQYEGCCSVD"
     gene            complement(966..>3431)
                     /locus_tag="VKU83_07475"
     CDS             complement(966..>3431)
                     /locus_tag="VKU83_07475"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLI93432.1"
                     /translation="SILVKGTTRGVTAGGDGKYVLELSPGAYTLVCQYVGYARREKKI
                     SVGETAEVVDFRLNLQQLSMAEVVVRPGGEDPAYAIIRHAIRKRKDYESPLDSFTCEA
                     YVKTLIRTRRLPDRIFGQRLQEKDKKEMGVDSAGKGIIYLSESLTKINYKKPDKIKLE
                     ILSGREAGGNGFGFSFPVFINFYENNVNALGGPLGPRGYVSPIADGALNYYRYHFLGS
                     YFEDGREINRIRVIPRRKYEPLFSGTIEITEGDWRIHSLDLLLLKTSQLQILDTLEIR
                     QIHGPVDSGSLSAGAGIWQVRNQVVYFTFDILGIDAVGNFLDVYNDFDLNPRFHKRFF
                     NHVLIRYDTAGNRHSPAYWDSIRPVPLQPDEKLNYRTRDSIYRYNRDSMGTRKNRDSL
                     LKQQGPVKPGQLLLTGFQRSDFRQPRPLTYSLDPLLPSLEYNTVEGINMKLSGSLSRP
                     VGRGRISLLPHLRYGFHDTRLHAWAELRWSRRNFSLDGDEASSSRQTWSVSGGKRVSQ
                     FNPDNPISEWVNTLYTLLARQNYMKIYDNYFVQLRSATRFDNGLRLTASALYEDRMPM
                     ANTTDYSLFTVKGQSFTPNYPVEKLVAPFPRHQAVLTGIGIEYQPGQRFIEFPDRKVS
                     IGSDYPTMEARYEKGWNGPLGSDVNFDKWRFSVWGDLNLKLRGLLRYRFGIGGFLNSN
                     SVYIQDYQHFNGNQTVVASEYLNSFQLAPYYANSTIAGFYTTGHLEQHFNGLLTNKIP
                     VFRKLNWNLVGGVNAFYVSGNNNYAEAFGGLENIFKLFRVDLVGSWLNGHYSQTGVRV
                     GFDGLLGSGIRQAGNNGGPPPRP"
BASE COUNT          722 a          955 c          992 g          762 t
ORIGIN      
        1 aatcaacgct gcagcagcct tcgtattggg cagcacattc gttgcattga ataaacaaca
       61 ggtggcaggc gtcattccgg caattggtat gggtgtcggc gggccggccg cattgatggc
      121 aggctgcaat cacttccgcg ctgatccgct cccccagccg gtcatcaaag acaaaatttt
      181 ttcccctgaa tttgttgtcc agcccttttt cctttaccgt attggcgtaa tggatgatgc
      241 ccccttccag gtgatatacc ttgctgaacc ctttgtgcag cagccaggcg ctggctttct
      301 cacatctgat accgccggtg cagtacatga tgatcgggct gtctttcttg tctttcagga
      361 gttcggcgga cattgggagc tgttcccgga acgtgtcgga ggggatctgg atcgcccggt
      421 cgaaatggcc gacctcaaac tcgtaatggt tacgcatgtc gacaatgacg gtggcgggat
      481 cggcagccag ctggttgaac gcagccgcgt ctacatattt ccctttacgg ctcatgtcga
      541 acgaaggatc atggataccg tcggcgacga tctttttcct gaccttgatc tttagcaccc
      601 agaacgattt cccgtcgtcg tcgaaagcgc gattgagcct gagggcctcc aatcccggaa
      661 tggattcgag gcgggacttc agttgctcca gccgggaggc cggaacgctg acctgagcat
      721 tgatcccctc ggccgccacg tagatgcggc caaataccgc cagtacatgc agttccacgt
      781 aaagcatgtc ccggaatgcg gcagggtcgg cgatgggcac ataccgataa aacgaaaggg
      841 tgatccgctg ctctttttcc tcgagcagcc gttgtttcag ctctttttga gaaacccggt
      901 tatgtaacaa ggccatccgg caaaagtacg gcttttccgg cagtcgccgg atcccatggc
      961 cccggtcagg gtcgcggtgg cgggccgcca ttgttccctg cctggcgaat gccgctgccg
     1021 agcagaccgt caaatccgac ccggacgccc gtctggctat agtgtccgtt cagccaggaa
     1081 ccgacgaggt ctacgcggaa cagcttgaag atattctcta ggccgccgaa ggcctccgcg
     1141 tagttattat ttccactgac ataaaacgca ttgacgcctc cgacaaggtt ccagttcaat
     1201 tttctgaaaa ccgggatctt attcgtcagc aagccgttaa aatgttgttc cagatgtcct
     1261 gttgtgtaaa agcccgcgat agtactgttg gcgtaatatg gcgcaagctg aaagctgttg
     1321 aggtattcgc tggcaacgac ggtctggtta ccattgaaat gttgatagtc ctggatatac
     1381 accgaattgc tgttgaggaa accgccgatc ccgaaccggt atcgcagcag accccggagc
     1441 ttgaggttca aatcccccca gaccgagaag cgccacttgt cgaagttcac gtcgctgccc
     1501 agggggccat tccagccctt ttcataacgg gcttccatgg taggataatc ggagccgata
     1561 gacactttcc ggtcagggaa ttcgataaag cgctggccgg gctgatattc gatcccgatt
     1621 cccgtcaaaa cggcctggtg ccgcggaaag ggcgcgacga gcttctccac ggggtaattc
     1681 ggcgtgaagg actgcccttt aacggtgaac agcgaatagt ccgtggtgtt ggccatgggc
     1741 atgcggtctt cgtacagggc gctggcggtg aggcggaggc cgttgtcgaa acgggtggcg
     1801 gacctgagtt gcacgaaata gttgtcgtat atcttcatgt agttctggcg cgccagcaaa
     1861 gtgtagagcg tgttgaccca ttcggagatg gggttatcgg gattgaactg ggaaacgcgc
     1921 ttgcccccgg agacagacca ggtctgccgc gacgagctcg cctcatcccc atccaggctg
     1981 aaattccggc ggctccaacg aagctcggcc caggcgtgca gtcgcgtatc gtggaagcca
     2041 taccgcaggt gaggaagcag actgatcctt cctctcccga cagggcggct cagcgagccg
     2101 ctaagcttca tgttgatacc ctcgaccgtg ttgtattcga gcgaggggag cagcgggtcc
     2161 agggagtagg tcagcggccg cggctgccgg aaatcggacc gctggaaacc cgtcagcagg
     2221 agctggcccg gctttaccgg accctgctgc ttcaataatg agtcgcggtt cttccgggtt
     2281 cccatcgaat cccggttata ccggtaaata ctgtcgcgcg tccggtaatt cagcttctca
     2341 tccggctgaa gggggaccgg cctgatggaa tcccaataag ccgggctgtg tctgttgccg
     2401 gccgtatcgt agcggatcag gacatgattg aaaaagcgct tgtgaaaccg cgggttcagg
     2461 tcgaaatcgt tgtaaacgtc gaggaagttg cccaccgcat cgatcccgag gatatcaaac
     2521 gtaaaataga ctacctggtt tctgacctgc caaatgccgg cgcccgcaga aagggagccg
     2581 gaatcgacgg gcccatggat ctggcggatc tcgagggtat ccaggatctg taactgggag
     2641 gttttgagca acagaaggtc aaggctatgg atccgccagt ctccctccgt gatctcgatc
     2701 gtccccgaga atagcggttc gtatttccgg cgggggatca cccggatccg gttgatctcg
     2761 cggccgtctt cgaagtaaga accgaggaag tgataacggt aataattgag cgccccatcg
     2821 gcgataggcg aaacgtatcc acgcgggccg agcgggccgc ccagggcgtt gacattattt
     2881 tcgtagaagt tgatgaatac gggaaacgaa aagccgaagc cgttgccgcc cgcttcccgg
     2941 ccggatagga tctcgagctt gatcttgtcg ggctttttat agttgatctt ggtcagcgat
     3001 tcggaaagat agatgatccc cttccctgcc gaatctacgc ccatttcctt cttgtccttt
     3061 tcctggagcc tttggccaaa gatccggtcg ggtaaccgcc gcgtccggat cagcgtcttg
     3121 acataggcct cacaggtaaa ggaatcgagc ggcgactcgt aatcctttct tttcctgatg
     3181 gcatgacgga tgatggcata agcggggtcc tcgccccctg gccggacgac gacctccgcc
     3241 atggaaagct gctggaggtt cagccggaaa tcgacgacct ccgcggtctc gcccaccgaa
     3301 atcttctttt cgcgccgggc atatcccaca tattggcaga ccagggtgta cgcgcccggg
     3361 gacaattcca gaacatactt gccgtcgccc ccggcagtaa caccgcgggt cgttcctttc
     3421 accaggatcg a
//
LOCUS       DATGKE010000433         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_500537, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000433 DATGKE010000000
VERSION     DATGKE010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500537"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VKU83_07480"
     CDS             <1..364
                     /locus_tag="VKU83_07480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI93433.1"
                     /translation="DVLAASDIYVLPSLWEAFPIALLEAMSMGKAVIGTNVDGTPEII
                     RDRENGLLIEIDDLEANLESSLLELCSDAGLRERLQTSAINSIYNKYTVENLARKNEN
                     IYQELVRPAPVMKSPAIG"
     gene            822..>2503
                     /locus_tag="VKU83_07485"
     CDS             822..>2503
                     /locus_tag="VKU83_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93434.1"
                     /translation="MKISTRVAFLLLTVFTVVFAQDARAQLAMSPTDSVYTYSSTAPL
                     GSVTNPNQPPTGKIGKWIRTVRMSWNTNEYKAYIYNGSDFRIHFPKSYNPAANDGKRY
                     PILVFYCGDGEFAAIHDNEDQLAHGAQPFQAQIDAGNFDGYAVFMQTQYGWGTAQFLN
                     YEALIDSLCTYYKGDPYRVTQNGLSGGGQGIWNNIAQAPAYFPGCIPMSATLTADGTQ
                     AVINLVKFTPIWNLDGGKDNDPPPSLAYAVQDSMLKYGANYTLYEFPTLGHDTWDSTW
                     LKPGFWPYCNNVYESNPWPLYGRTNFCPGQKINVTIGVVPGLAGYQWRRNDTIIPGAT
                     GNTIVATQPGSYDCQVKRGNYYWSDWSHKPVVITVLGSSQTPPITTVNNASAVLPDAA
                     GDTIVTLQMPPGDSLYTWKYLATGATVGSHQTLTVGASKTGDYVAYALPFQGCTSVAS
                     PAFPVVKASGPNPPPPATTLVATPLGFTSARLTWSAAPNPTHPPTAYEIYRGTKSGVY
                     QLVAQAAYDSLGYTDATVSPGGKYFYLVRAVDTTGAAVPSNQTSVTMPGDVTP"
BASE COUNT          548 a          690 c          676 g          589 t
ORIGIN      
        1 cgatgtgctg gcagcttcag atatctatgt gttgccgtca ttgtgggagg cgttcccgat
       61 cgcgctgctc gaagccatgt caatgggaaa ggcggtgatc ggcaccaatg tggatggaac
      121 gccggaaatt attcgcgaca gggagaacgg gttgctgatc gagatcgatg acctggaggc
      181 taacctggaa tcgtctttgc tggagctttg cagcgatgcg gggctgcgtg aacggctgca
      241 aacgagtgct attaacagta tttataataa gtatacggtt gagaaccttg cccggaagaa
      301 cgagaatatt taccaggaac tggttcggcc cgcgcctgtg atgaaaagcc cggcaattgg
      361 atgatggcgg cggccggaaa tgccggtgaa tgttgggttt ctcccggcgg aacgatttaa
      421 aatttgcaca gaaaaacttg caggatcctg aacgaaattg tatttttgcg acggctatgt
      481 gccctacaat tgtttgatcg tctaatttcc cctgataacc aatctttctt ctctcctttt
      541 tccttctgaa cacctcaata ttctattcga ctaccgccaa tttctatgca acgccggcag
      601 tttgagcgct gctttgcagt ttcccagttt tttagatccg atccatcttg ctttcccttt
      661 ttgcccgtac ccggaagcag gaggtgaaaa taatgagcgc agtgctgtgg cccctccgtc
      721 aaggatgtca ccgcgcggcg gccgtgcgga tgagtcagat actttttcta aactttttta
      781 taattaaacc ctagacattg gcccctttaa ccttaagaat catgaaaatt tctacccgcg
      841 tagcttttct cctgctcacc gtttttacgg tcgttttcgc gcaggatgca agagctcagc
      901 ttgccatgag cccgaccgac tcggtgtata cgtatagctc gaccgcgccg ctcggctccg
      961 taaccaaccc gaaccagcct cccaccggaa agatcgggaa gtggatccgt acggttcgga
     1021 tgtcgtggaa cacgaatgaa tacaaggcct atatctataa tggatccgat ttccggattc
     1081 acttccccaa gtcgtataat ccggctgcca atgacggcaa gagatatccg atcctcgtct
     1141 tttattgcgg cgatggtgaa tttgccgcta tccatgacaa cgaagatcag ctggcgcatg
     1201 gagcgcaacc gttccaggcg cagatcgacg ctggcaattt cgacggctat gcggttttca
     1261 tgcagaccca atacggttgg ggcaccgcgc agttccttaa ttacgaggct ttgatcgatt
     1321 ccctgtgcac gtattataaa ggcgatcctt accgtgttac ccaaaacggg ctttcgggag
     1381 gcggtcaggg tatctggaat aatatcgcgc aggcgcccgc ctatttcccg ggctgcattc
     1441 ccatgtccgc gacgcttacg gcggatggga cgcaggcagt gatcaacctg gtcaaattca
     1501 cgccgatctg gaacctggat ggaggaaagg acaacgatcc ccctccgtcc ctggcttatg
     1561 cagtccagga ctccatgttg aagtacggcg cgaattatac cctttatgaa tttccaacac
     1621 tcggtcatga tacctgggac agcacctggc tcaagccggg attctggccc tattgtaata
     1681 atgtttatga gtccaacccc tggccccttt acggccggac gaatttctgt cccggccaga
     1741 agatcaatgt gacgatcggc gtggtgcccg gtttggccgg ttaccaatgg cggcggaatg
     1801 acaccattat cccgggtgcg acgggcaata cgattgtcgc cacccagccg ggtagttatg
     1861 actgccaggt caaacggggc aattactatt ggtcggactg gtcgcacaag cctgtggtca
     1921 ttacggtcct cggttcgagc cagacgccgc ccattacgac ggttaacaat gcaagtgctg
     1981 tactgccgga tgccgcgggg gatacgattg taacgctgca aatgccccca ggcgattcgc
     2041 tgtatacgtg gaaatatctg gcaacgggag caacggtagg ttcccatcag acgctgacgg
     2101 tgggcgcgag caagaccgga gactatgtag cttatgccct tcccttccag ggctgcacga
     2161 gcgtcgcttc acccgcattc ccggtcgtga aggcgagcgg gcccaatccg ccgccgccgg
     2221 caactaccct ggtggcgacg ccattaggtt tcacctcggc ccggctgacc tggtccgcag
     2281 cacccaatcc gacccatccg ccgacggcgt atgagatcta ccgcggcacc aagagcgggg
     2341 tataccaact ggttgcccag gcagcgtacg actcgctggg gtatacggat gcgaccgtga
     2401 gcccgggagg aaaatacttt tatctcgtcc gtgccgtgga taccaccggt gcggccgtac
     2461 cgtccaacca gacctccgtt actatgcccg gcgatgtcac gcc
//
LOCUS       DATGKE010000434         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_14628, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000434 DATGKE010000000
VERSION     DATGKE010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14628"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VKU83_07490"
     CDS             <1..850
                     /locus_tag="VKU83_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HLI93435.1"
                     /translation="LVRELLRRNIPESIGIVAFSQEQQQAIAAALNTLAAEDVTFAQQ
                     LEEAFNRTDNDMFTGLIIKNLENIQGDERDIVLISVCYGPDSRGRMLMNFGPVNKRGG
                     EKRLNVLFSRARKHMAVVSSIRYEQITNEYNEGANYLRRFLHYAELISEGKMDTARTI
                     LDSLAPHKAETAVSQTVIRAQLKEHLEGMGYTVDGPVGQSDFKCSLAIKQRPEDEDYA
                     LAILIDDEGHYRNGNLIEQYYQRPAILESFGWKVLPVYAKDWLHQPQKVLDQIVRMLP
                     ASQEPR"
     gene            complement(835..993)
                     /locus_tag="VKU83_07495"
     CDS             complement(835..993)
                     /locus_tag="VKU83_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93436.1"
                     /translation="MALIPNNKKKKDGKQIAGAKSNIPGFPAPKGKANSKGVAKNTRF
                     TGGAQRGS"
     gene            1044..1406
                     /locus_tag="VKU83_07500"
     CDS             1044..1406
                     /locus_tag="VKU83_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93437.1"
                     /translation="MPKELYFDISSEDSGGSLFRVPEPDGGVAFLYNYSVADARTGEV
                     EVFDTWYVSFEAFWKALKKDPLWYYRHPLFVHPEQRAFVAGELKGVNWAVYPDKKWQE
                     SHQRQWKKVLTDPAAYYH"
     gene            complement(1442..1717)
                     /locus_tag="VKU83_07505"
     CDS             complement(1442..1717)
                     /locus_tag="VKU83_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93438.1"
                     /translation="MTKPLVDLTRELLSSGVFSHLPDNEIARLHWMILQGQRDEPKSF
                     QPLFSYWYRGDYYSSPASLRLLQQCNEYLQRMGQPLIDVYGEEFYEA"
     gene            complement(1830..2318)
                     /locus_tag="VKU83_07510"
     CDS             complement(1830..2318)
                     /locus_tag="VKU83_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HLI93439.1"
                     /translation="MKQWIAFAGIALIAACKSPADHPSPVRDTLVIAASLPLAADTTT
                     LGGVWYLQPVLASDTASGKRPILVFDLAKTRFSGNTGCNSMHGTFWFSKEDSSLSFSD
                     KIVLTKMACPGYNELGFLKSLRSTGRYRLQQGVLTLLSDDHVELSNWTRKPTTAPKAL
                     KA"
     gene            2441..>3025
                     /locus_tag="VKU83_07515"
     CDS             2441..>3025
                     /locus_tag="VKU83_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLI93440.1"
                     /translation="MLKDGLLLIVSLLFLVSMLAMISDKLRISYPIFLVIAGLGISLI
                     PGIPTIRLDPNTVFVIFLPPLLYSAAWNTSWHEFWQLRRPIGLLGFGLVFCTALAIAW
                     FSSWLIPDFSLALGFLLGGIVSPPDAVAATSVLHSLRVPKRVVTILEGESLVNDAASL
                     IVFRFALVAILTGEFRLWKAEVDFLLVIFLGIVIG"
BASE COUNT          650 a          773 c          883 g          719 t
ORIGIN      
        1 gctcgtacgg gagctgctgc gacgcaacat ccccgaaagt atcggcatcg tcgccttcag
       61 ccaggagcag caacaggcga tcgcggcggc attgaacaca ttagccgcgg aggatgtaac
      121 attcgcccag caattggaag aagcttttaa ccggaccgat aacgatatgt tcaccggtct
      181 tatcatcaag aacctggaga atatccaggg cgatgaacgg gatatcgtgc tcatcagcgt
      241 gtgttatggt cccgacagcc ggggcaggat gcttatgaat ttcgggcccg tcaacaaacg
      301 gggcggcgag aaacggctga atgtcctgtt cagccgggcg cgtaaacaca tggctgtcgt
      361 gagcagcatc cgttacgaac agatcaccaa tgaatacaat gaaggcgcca attacctgcg
      421 gcgcttcctg cactatgccg aattgatcag cgaagggaag atggacacgg ccaggaccat
      481 attggacagc ctggcgccgc ataaggcgga aaccgccgtt tcgcaaaccg tgatccgcgc
      541 acagttaaaa gaacacctgg aaggcatggg ctatacggtg gacggccctg tcggacagtc
      601 ggatttcaaa tgttcactgg cgataaaaca gcggcccgaa gacgaagact atgcactggc
      661 catcctgatc gatgacgagg gccactatcg caacgggaac ctgatcgaac aatactatca
      721 gcggccggct attctggaaa gcttcggctg gaaggtcttg ccggtgtatg ccaaggattg
      781 gctgcatcag ccgcagaaag tattagatca gatcgtgcgg atgcttcccg cctctcagga
      841 gccgcgttga gcaccgcctg tgaagcgtgt attcttggct acgcctttgc tgttggcctt
      901 acctttggga gcggggaaac cggggatatt gctcttggcg ccggcaattt gtttgccgtc
      961 ctttttcttt ttattgttgg ggatgagagc catatgtata atatttttcg ttatgggata
     1021 tttcaggatg aagatacaaa attatgccta aagaactcta tttcgatatc agctctgagg
     1081 acagcggcgg cagcctcttc cgggtgccgg aaccggacgg cggggttgct tttttataca
     1141 actacagcgt tgcagacgcg cggaccggcg aggtggaggt tttcgatacc tggtatgtga
     1201 gcttcgaagc tttttggaag gcgttgaaaa aggacccgct ttggtactat cggcatcctt
     1261 tgtttgtgca tccggagcag cgggcattcg tagcggggga attgaagggg gtgaactggg
     1321 cggtatatcc cgataaaaaa tggcaggaaa gtcatcagcg gcagtggaag aaggtgttga
     1381 ccgatcccgc tgcctactat cactgaccgg cccgaggccg gtcggccgaa ggcctgtctg
     1441 atcaggcctc gtagaactct tccccgtata cgtctatcaa cggctgcccc atgcgctgaa
     1501 ggtactcgtt gcattgctgg aggagccgca acgaagcggg ggaagaataa taatcacccc
     1561 ggtaccagta gctgaatagc ggctgaaaag acttgggctc gtcccgttgt ccctgaagga
     1621 tcatccagtg cagccgggca atctcattgt ccggaagatg gctgaagacg ccgctgctta
     1681 gaagttcccg ggtcagatcg accaatggtt ttgtcataat gtgtgatttc ctgaagttcc
     1741 ggcaattata ttaccaaaat gtgaacctta tgttatccta gtgtaaccac ctttgtttgt
     1801 gcattacgtg tggatagccg gcctgcggct tatgccttga gggccttggg ggcggtggtg
     1861 ggttttcgtg tccagttgct tagctcgaca tggtcgtcgg aaagcaaggt aaggacgcct
     1921 tgttgtaatc ggtaacggcc cgtgctgcga aggcttttta gaaaacccaa ttcattatag
     1981 cccggacagg ccatcttcgt gagcacgatc ttatcggaga aggacaggct gctgtcttcc
     2041 ttgctgaacc agaatgttcc gtgcatgctg ttgcagccgg tattgcccga gaaccgcgtt
     2101 ttggcgagat cgaataccag gatgggtctt ttccccgaag ccgtgtccga agccagcacc
     2161 ggctgtagat accacacgcc gcctaacgtg gtcgtatctg ccgccagcgg caacgaagcg
     2221 gcgatcacca gggtatcccg tacaggagaa ggatgatcgg ccggtgactt gcaagcagcg
     2281 atgagagcga tgccggcaaa ggcaatccat tgcttcatag ttgagggttt ttgctagcaa
     2341 gggcaaaaaa aacatgccac cccgatcggg gaatttaatc cccggggcag tcccggtttc
     2401 gtggcgttcg gcggttcaga ataagacatt atcttacggt atgttaaagg atggcctcct
     2461 gcttatcgtt tccctgctgt tcctcgtctc catgctggcg atgatcagcg acaagctgcg
     2521 catctcctat cctatctttc tggtgatcgc cggtttgggc atcagcctca tccccggaat
     2581 tccgaccatc cggctcgatc ccaataccgt gttcgtcata ttcctgccgc ctttattata
     2641 cagtgccgcg tggaatacgt cctggcatga gttctggcag ctgcggcgtc cgatcgggct
     2701 gctgggtttc ggactggtct tctgcaccgc tctggcgatc gcctggttct ccagctggct
     2761 gatccccgat ttctcgctgg cgctgggttt cctgctgggg ggtatcgtgt cgccgccgga
     2821 cgcggtagcc gctacgagcg ttctgcatag cctgcgggtt cccaaacggg tagtgaccat
     2881 tctggagggc gagagcctgg tcaacgacgc cgcgagcctt atcgttttcc ggtttgcgct
     2941 tgtggccatc ctgacgggag agttccgcct ttggaaagcc gaagtagatt tcctgctcgt
     3001 catttttctg gggatagtga tcggg
//
LOCUS       DATGKE010000435         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_32270, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000435 DATGKE010000000
VERSION     DATGKE010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32270"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VKU83_07520"
     CDS             <1..858
                     /locus_tag="VKU83_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93441.1"
                     /translation="KRLSSVQIELQDIAGEVDRINDSVHYDPRRIEEINERLSLGYRL
                     LKKHGLRTTAELLEAQRRLESSLQDSLQMGERIVAAEAAVGRLLQETRMKAAALSTGR
                     KAQVGGLEAKVNQLLKQVGMPNARLRVVVEAAELNPSGADAVEFLFDANNNNRFEPVR
                     KVASGGELSRLMLCIKSLVAESIDLATLLFDEIDTGISGEAARQVGIILKGLAAKRQV
                     ICITHQPQIAGKADSHYYVYKQAGGAGIQTKVRLLDREERITAIARMLGGEKPTAAAL
                     ENAREMVIP"
     gene            892..1098
                     /locus_tag="VKU83_07525"
     CDS             892..1098
                     /locus_tag="VKU83_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93442.1"
                     /translation="MKERSIAILLVLLVSMVPLFYFNRWLQRVMHPRENVSRLFIFLL
                     ANFVLIVAYTVLVVGVIAKIFPLR"
     gene            1145..1957
                     /locus_tag="VKU83_07530"
     CDS             1145..1957
                     /locus_tag="VKU83_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93443.1"
                     /translation="MAYNLLKGKKGIIFGALDERSIAWRTALRCHEEGAQLVLTNAPV
                     ALRMGEINKLAEAVKAPVIGADVTNMDDLKKLFEESMKHFGGGIDFILHSIGMSLNVR
                     KGKSYTEIDYEWNKKTLDISAMSLHRVLRTAWDLDALTEGASVVALTYIAAQRVFPDY
                     NEMADAKALLESVTRSFGYYYGIKKKVRVNTVSQSPTRTTAGSGVKGFDGFIAYAEKM
                     SPLGNASADECAGYCAVLFSDLTKMVTMQNLFHDGGFSFTGVTEAIVNQMEK"
     gene            complement(1993..2214)
                     /locus_tag="VKU83_07535"
     CDS             complement(1993..2214)
                     /locus_tag="VKU83_07535"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HLI93444.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVVENLQELE
                     DEGEIYEGIDDIWPDYPSQEDFFFNEDEY"
     gene            2291..2950
                     /locus_tag="VKU83_07540"
     CDS             2291..2950
                     /locus_tag="VKU83_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93445.1"
                     /translation="MLKARGIQKYYGKLWVLKGVDVDISKGEIVSIVGPSGCGKSTLL
                     HILGTLDEAAEGSIFLQQREIQQLKGEALAAFRNRHIGFVFQFHHLLPEFTALENVSI
                     PAWIAGGRKKEVERRALALLESLGVGHRVDNKPGELSGGEQQRVAVARALINNPDIVF
                     ADEPTGNLDTANAKDLHELFFDLRKRFQQTFLIVTHNEELATMSDRVLHMKDGKIVAA
                     G"
     gene            complement(2947..>3350)
                     /locus_tag="VKU83_07545"
     CDS             complement(2947..>3350)
                     /locus_tag="VKU83_07545"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLI93446.1"
                     /translation="GTATPLLRVIQDRLFTIGASLACDPEKEPKLKIPDLKEDDISTL
                     EKEIDRMDSELPPLKAFLLPGGHVAVSTAHITRCVCRRAERACVRLQENRAFVEPLVL
                     QYLNRLSDYLFVLARFIARNLFVDEIAWKPR"
BASE COUNT          782 a          758 c         1071 g          739 t
ORIGIN      
        1 aagcggttat cctccgtgca gatcgagttg caggatatcg ccggggaggt ggatcgcatc
       61 aatgacagcg tccattacga cccgcggagg atcgaagaga tcaacgagcg gttatcgttg
      121 gggtaccgcc tactgaaaaa acatggcttg cgaacgaccg ctgaattgct cgaagcacaa
      181 cgccggctgg aaagcagctt gcaggattct ttgcaaatgg gtgagcgtat tgttgcggcg
      241 gaagcagcgg tagggcgttt gttgcaggaa accaggatga aggcagccgc cctgtcgact
      301 gggcggaagg cccaggttgg cggcctggag gcgaaggtca atcagctttt gaagcaggtg
      361 ggaatgccga atgcccggct tcgggtggtg gtcgaggctg ccgaactgaa cccgtcgggt
      421 gcagacgcgg tcgaatttct gtttgacgca aacaacaaca accggttcga gcctgtccgg
      481 aaggtcgctt cgggcgggga actaagcagg ctgatgctct gcatcaaatc cctggtcgct
      541 gagtcgatcg atctggcgac cctgctattc gatgagatcg acacggggat ctccggcgag
      601 gcggcgcgac aggtgggtat cattctgaag ggcctggcgg caaagcggca ggtgatctgt
      661 atcacgcacc agccacagat cgcgggaaaa gcggacagcc attattatgt atataaacag
      721 gccggcggag cgggcattca gacgaaggtc aggttattgg accgcgagga gcggatcacg
      781 gcgatcgccc gcatgctggg tggagagaag ccgacggcgg cggcgctcga gaatgcgcgg
      841 gaaatggtga tcccatgaaa cggtggcatc cggccaggta aagtgacggg catgaaggaa
      901 cgttcgatag cgatattgtt ggtgttgctg gtatccatgg tgccgctctt ttattttaac
      961 cgctggttgc agcgggtgat gcacccgcgt gagaacgtaa gccggctgtt catttttctt
     1021 ttagccaatt ttgtgctgat cgtggcgtat acggtgttgg tggtgggcgt gatcgcaaag
     1081 atttttccgc tgcggtgaaa tgggcagagc cctgcgatca cctatatttg cgaaaactca
     1141 gtctatggca tataacctct taaaagggaa aaaagggatc attttcgggg ctttggatga
     1201 gcggtcgatt gcatggcgga cggcgctgag gtgtcatgaa gaaggggctc agctggtatt
     1261 gacgaacgcg ccggtggccc tgcggatggg agagatcaac aagctggcgg aggcagtgaa
     1321 ggcgccggtg attggcgcag atgtgacgaa tatggacgat ttgaaaaaat tattcgagga
     1381 atcgatgaaa catttcgggg gtgggatcga tttcattctg cattcgatcg gaatgagcct
     1441 gaatgtgcgg aaaggcaaga gttataccga gatcgactac gagtggaata agaagacgct
     1501 cgatatttca gccatgtcgt tgcaccgggt attgagaacc gcctgggacc tggacgcgct
     1561 gactgagggc gcaagcgtcg tggcgcttac gtatattgca gcgcagcggg tattcccgga
     1621 ttacaatgaa atggcggatg ccaaagcctt actggaaagc gttacccgca gtttcggcta
     1681 ttattacggg ataaagaaaa aagtgcgggt caacacggtt tcccagtcgc cgacgcggac
     1741 gacggcgggc agcggcgtga agggcttcga cggtttcatt gcatatgccg agaagatgag
     1801 cccgttgggg aacgcgtcag cggatgagtg cgcaggttac tgtgcggtct tgttcagcga
     1861 tttgacgaag atggtgacga tgcaaaacct tttccacgac ggaggatttt cgtttacggg
     1921 agtgacggag gcgatcgtca accagatgga aaagtaggtg ggtgcgaatt taattaatat
     1981 gtaattgcct gactagtatt cgtcttcatt gaagaaaaag tcttcctggg aaggataatc
     2041 gggccagatg tcatcgatgc cttcgtaaat ctcgccttca tcctccagtt cctgcagatt
     2101 ttctaccact tcgatcggtg cgccggagcg gatggcataa tcgatcagtt catccttggt
     2161 agccggccag ggggcatctt ccaggtagct ggccaattcg agggtccaga acattagtcc
     2221 aattttccgc aaaagtaatt tttaaaacga aaccaccaaa taacagttaa aatttaacca
     2281 gattttgatt atgctaaaag ccagggggat acaaaaatat tatggcaagt tatgggtatt
     2341 gaaaggggtg gatgtcgaca tctcgaaggg cgagatagtg agtatcgtag gcccttcggg
     2401 ctgtggtaaa agtactttgc tgcatatcct tgggacgctg gatgaagcag ctgaaggatc
     2461 catctttttg cagcagcggg aaatccagca gcttaaggga gaggcgttgg cggctttccg
     2521 taaccggcac attggttttg tttttcaatt tcatcatttg ctgccggagt tcaccgcgct
     2581 ggagaatgtc agtattcccg cgtggatcgc gggtggccgc aaaaaagaag tcgaacgcag
     2641 ggcgttggcc ttgctggaaa gtctcggggt ggggcaccgg gttgacaata agcccggcga
     2701 gctgtcaggc ggggagcagc agagagtggc ggtcgcgaga gcgttgatca acaacccgga
     2761 tatcgtgttc gcggatgagc cgaccggcaa tttggatacg gccaatgcaa aggacctgca
     2821 tgaactgttt ttcgatctga ggaaacgctt ccagcaaaca ttcctgatcg tgacgcataa
     2881 tgaagagctg gcgacgatga gcgaccgtgt gctgcatatg aaagacggga agatcgtggc
     2941 cgccggctag cggggcttcc acgcgatttc gtcgacgaac aggttgcggg cgatgaaacg
     3001 cgcaaggacg aacagataat cgctgagccg gttgagatat tgcagtacca gcggttcgac
     3061 gaacgcccgg ttttcctgca ggcgcacaca ggccctttct gcgcggcggc agacgcaacg
     3121 ggtgatatgg gcggtcgaga ccgcaacatg tccgcccggc agcagaaagg ctttcagcgg
     3181 cggcagctcg gaatccatcc ggtcaatttc cttttccagc gtgctgatat cgtcttcttt
     3241 caggtccggg atcttgagct tcggttcctt ttcggggtcg caggcgagcg acgcgccgat
     3301 ggtgaaaagc ctgtcctgga tgactctcag gagcggcgtt gccgttccgg
//
LOCUS       DATGKE010000436         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_397835, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000436 DATGKE010000000
VERSION     DATGKE010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397835"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VKU83_07550"
     CDS             complement(<1..645)
                     /locus_tag="VKU83_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLI93447.1"
                     /translation="MKKTLLFLLVAAATTAYAQKTPLPVIFDTDMGPDYDDVGAITLL
                     HAFADSGKATILATMASNKYEGIAGVIDVFNTYFGKPNIPIGIPRSWAVNERDSQHWT
                     DSILAYYPHRITRNDQAEDAVKLYRRILAGQPAQSVVIITTGFLTNLHNLLKSPADSY
                     SPLDGRQLVKQKVKQLVAMAGKFPQGWEFNVMRDSIASADVFDNWPTPILLSGFE"
     gene            787..1116
                     /locus_tag="VKU83_07555"
     CDS             787..1116
                     /locus_tag="VKU83_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93448.1"
                     /translation="MKTLIATTLALFIAGASFAQGPGHQPTHLQNHPRVNQVNRRIDR
                     QEHRINTERKEGEISHRQAQQDRRNLKAVNQEKHDMRKEDNGHLTKTDQRALNQQLNK
                     NSRKIGH"
     gene            1150..>2140
                     /locus_tag="VKU83_07560"
     CDS             1150..>2140
                     /locus_tag="VKU83_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93449.1"
                     /translation="MFNKIYPFFLFIFFAQAAYSNGRPFPGYYITINGDSVSCLIEYN
                     DWFRNPSSIVVDVNGARKTLRPTDINRFGVTGYADYQSANVTYHLGPVQGFNLTAEYS
                     DSTDTKDCFLLILVKGPYSLYELKLAEREYFFLSENGGPIKELVYRVKQSQMRISSDD
                     QYKTVLANSMIREHIWDDQDPSAITGLTYNKKKIIALVDRLNEARTGVKASLSPPAKF
                     GESGVQLEIFGGAVLNLFPSSFSTEFGTGKFPATFSGSGGLGLHVAIPGHFNAFSVGL
                     TLGYSSFQSSSSMAGTAKVNVQSDTWYTLATYTEHPAVSNSLLTTNLYVMYSLP"
BASE COUNT          502 a          538 c          583 g          517 t
ORIGIN      
        1 ctcgaagccg ctcaggagga tgggggtggg ccagttgtca aatacatcgg ccgacgcgat
       61 gctgtcccgc atgacgttga actcccaacc ctgcgggaat ttgccggcca tggcgacgag
      121 ctgtttgacc ttttgtttta cgagctgcct gccatcgagc ggcgaatagc tatctgccgg
      181 actcttcagg agattatgaa gattggtgag aaagccggtt gtgatgatga cgacgctttg
      241 cgcgggctgg ccggctaaga tgcgccggta taatttcact gcatcttcgg cctggtcatt
      301 acgggtgatg cggtgggggt agtatgcgag gatgctatcg gtccagtgtt gggagtcgcg
      361 ttcgttaacg gcccagctcc gggggatgcc gatgggtata ttgggcttac cgaaatacgt
      421 gttgaaaaca tcgatgacgc cggcgatgcc ttcatattta ttgctggcca tggtggcgag
      481 gatcgtggct ttgccggagt ctgcgaaggc gtgaaggagg gtgatggcgc ctacatcgtc
      541 atagtccggg cccatgtcgg tatcgaagat gacgggtaag ggcgtttttt gcgcgtaggc
      601 ggtggtggcg gcggcaacca ggaggaacag gagtgtcttt ttcatatccg gttttgttaa
      661 cgcatggtga aaatacaata agtataacac tttgcggtta ttaagggatt aaacccagag
      721 agaatctaac gatcctatat aagccgcctg tggcggcaca caaaaaccaa cttaaaatcg
      781 aaaaacatga aaacgctgat cgcaaccacg cttgccctgt ttatcgctgg cgcttctttt
      841 gcccagggcc cgggccatca acccacccat cttcaaaacc atccccgtgt gaatcaggtc
      901 aaccggcgca tcgaccgtca ggaacaccgc atcaacacgg aacggaagga aggtgagatc
      961 tcgcaccggc aagcccagca ggaccgccgc aatctaaagg ctgtcaacca ggagaagcac
     1021 gatatgcgca aggaagataa tggtcatctg acgaagaccg accaacgcgc tttgaatcaa
     1081 cagctcaata agaacagcag aaagatcgga cattaaggaa tcggaaatat tttcctattt
     1141 tgggggtaga tgtttaacaa aatctacccc tttttccttt ttattttctt cgcgcaggcg
     1201 gcctattcca acggccggcc gttccccggt tattatatca ctattaatgg cgattcggtc
     1261 tcttgcctaa tcgagtacaa cgattggttt agaaatccca gctccatcgt ggtcgacgtg
     1321 aatggcgcgc ggaaaacgct gcgacctacg gatatcaacc gtttcggggt gaccggttat
     1381 gcggattatc aatctgccaa cgtcacctat catctcggtc cggtacaagg gttcaacctg
     1441 acggctgaat attccgactc gaccgatacc aaggactgct ttttgctgat cctggtgaaa
     1501 gggccttatt ctttatatga actgaagctt gccgaacggg aatatttctt cctcagcgaa
     1561 aatggcggcc cgataaaaga acttgtctac cgggtgaagc aatcgcagat gcggatctcc
     1621 tccgatgacc agtataaaac ggttctggcc aattctatga tccgggagca tatttgggat
     1681 gatcaggatc cgtctgccat caccggcctc acctataaca agaaaaagat catagcactc
     1741 gtggaccggc tcaatgaagc gcgtaccggg gtgaaggcgt cgctctcgcc gccggcgaag
     1801 tttggggaga gcggggtaca gctggaaatc tttggcggtg ccgtattgaa cctttttccg
     1861 agttcctttt ctacggaatt cgggaccgga aaatttcctg ccactttttc gggctccggg
     1921 ggtcttggcc tgcatgttgc gatcccgggt catttcaacg ccttttccgt aggccttacc
     1981 ttaggttaca gtagcttcca atcgagctct tccatggccg gtaccgccaa ggtcaacgtt
     2041 caaagcgata cctggtacac cctggcgacc tatacggagc atcccgcggt ttccaattcc
     2101 ctgctcacga cgaatttgta tgtaatgtat tcgttgccgg
//
LOCUS       DATGKE010000437         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_146043, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000437 DATGKE010000000
VERSION     DATGKE010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146043"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VKU83_07565"
     CDS             complement(<1..288)
                     /locus_tag="VKU83_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93450.1"
                     /translation="MGQKKLIRFEAIKTFPNVLQYPAGMPGKWGAFFKNDNPLTLELA
                     CGKGEYTVGLARLYPNRNFLGVDVKGNRIYVGARAALESNLSNAAFLRTQID"
     gene            859..1329
                     /locus_tag="VKU83_07570"
     CDS             859..1329
                     /locus_tag="VKU83_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLI93451.1"
                     /translation="MAVKLNLDKLDLQIIHEMMDNAEISYAELGKKLFVSGGTIHVRI
                     KKLQELSVVKGTKLHVDLKQLGYDVIAFIGIYLEKSSLYDSVARELSKIPEIVRLNYT
                     TGNYSMFAEIVCKDINQLRFVLHDELQKIKGIERTETFISLEESFCRNVQVFKE"
     gene            complement(1372..>2014)
                     /locus_tag="VKU83_07575"
     CDS             complement(1372..>2014)
                     /locus_tag="VKU83_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93452.1"
                     /translation="GRDILSRLLIGVRVSLAVGLVTVLISLSVGVLLGALAGYFGGRV
                     DEVILWLVNIIWSIPTLLLVFAIMLVLGKGFWQVFIAVGLTLWVNIARIVRGQVLALR
                     EREFVEAARALGYSHFRILFRHILPNVMGPVWVVTASNFASAIVIEAGLSFLGVGVQP
                     PAPSWGLMIKENYNFIITHNPMLALAPGLAIMLLVLAFNLLGNSLRDILDVRN"
BASE COUNT          501 a          544 c          545 g          424 t
ORIGIN      
        1 gtcgatctgg gtgcggagga aagcggcgtt gctgagattg ctttcaaggg cggccctggc
       61 gccgacatag atccggtttc ctttcacatc cacgccgaga aaattgcggt tggggtaaag
      121 cctggccaga ccgaccgtat attcgccttt tccgcacgcg agctcgagag tgaggggatt
      181 gtcgtttttg aaaaatgcac cccatttgcc cggcattccc gccggatatt ggaggacatt
      241 ggggaaggtt ttgatggctt cgaagcggat aagtttcttt tggcccatga ggcgcaaaat
      301 taatgaatct tgggagctcg tcgcggccgg cggccggccg gcggggtgcc tttccactta
      361 attgggccga tttcggccca attaagatct gggacagtcc ccgggggcag tggcttcctc
      421 gccgcgcgcc ccttcaaccc tttcctctgg gaagtgcaaa aaaaagcccc gccttcggcg
      481 ggaccctagc tcatattctg ctgtagaggg aggagtcgaa cctccacggg gcagttagca
      541 gtagcgcaat gtgcggtggt caaccccagt cggacccgat tttagtcggg cccggcatta
      601 cactatgttt attccggatc cccacccccg agacaagagg gcatgtctgc caaatttcat
      661 cactccacat tctttgccgt cgcggtcctg gcttccgggc cgccggtcgg ccccggcgcc
      721 ctgccggata gcttgttgaa gcaaaactaa agaaacggca gaattatttg taattcttga
      781 acaaaaattt ttttattttc aatattattc aaatatttac agtataatgt agatatattt
      841 taaaaacccc ctctaaagat ggctgtaaaa ttgaatctcg acaaactgga tctgcagata
      901 atccacgaga tgatggacaa tgcagagatt tcttatgccg aattgggaaa gaaactattt
      961 gtatcgggag gcacgatcca cgtacggatc aagaagctgc aggaactgtc cgttgtcaaa
     1021 ggtacaaaat tacacgtaga cctgaaacaa ctcggctacg acgtcatcgc ctttatcggc
     1081 atctacctgg aaaaaagctc cctctatgac agtgtcgcgc gggagctcag caagataccc
     1141 gagatcgtgc ggctgaacta tacgaccgga aattacagca tgtttgcgga gatcgtctgc
     1201 aaggacatca atcagctgcg cttcgtattg cacgacgagt tgcagaagat aaagggcatc
     1261 gaacgaaccg agaccttcat ttcccttgag gaaagtttct gccggaatgt gcaggtcttt
     1321 aaagagtgag gccgatcccg atccgaccgc tggtcccggc gatcgcgatc cctagttcct
     1381 aacgtcgagg atatctctta atgagttgcc gagcaggtta aatgcgagca ccagcaacat
     1441 gatcgccagg ccgggtgcaa gcgccagcat gggattatgg gtgatgataa aattatagtt
     1501 ttccttgatc atcaggcccc agctcggcgc cgggggctgg acgccgacgc cgaggaagct
     1561 cagccccgcc tcgatgacaa tggcggaagc aaaattggaa gcagttacta cccaaaccgg
     1621 gcccatgaca ttcggcagga tatggcgaaa gaggatccgg aaatgggagt agccgagcgc
     1681 ccgggcggcc tcaacaaatt ctctttcccg caacgcgagg acctgaccgc ggacgatccg
     1741 cgcaatattg acccaaagcg tcaggccgac ggctataaaa acctgccaga agcctttgcc
     1801 cagcaccagc atgatggcaa agaccagcag cagcgtcggg atgctccaga tgatattgac
     1861 cagccaaagg atgacctcgt cgacgcgccc gccgaaatat cccgccaacg cgccaagcag
     1921 cactccgacg ctgagcgata tcagcaccgt caccagaccc acggcaaggc ttacgcggac
     1981 gccgatgagc agccggctga gaatatcccg gcca
//
LOCUS       DATGKE010000438         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1183697, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000438 DATGKE010000000
VERSION     DATGKE010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183697"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VKU83_07580"
     CDS             complement(<1..201)
                     /locus_tag="VKU83_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93453.1"
                     /translation="MKAFVNKSLCSWVKLNPTMSVRAALGQITGILRKYDPTQPFDYR
                     FVDAEYAKKFGAEERIGNIAAVF"
     gene            601..1032
                     /locus_tag="VKU83_07585"
     CDS             601..1032
                     /locus_tag="VKU83_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93454.1"
                     /translation="MNKKGPVIVIEDDHDDQEILGEIFEKLGYPNKVMFFKDGNVTLE
                     FLNQTDVHPFLILSDINMPAINGFELRNKIYTNEMLQTKCIPYLFFTTSSNRKEVEKA
                     YGLSVQGFFVKPNSFNELESTIKKIMEYWIECIAPSHYVVA"
     gene            1244..2830
                     /locus_tag="VKU83_07590"
     CDS             1244..2830
                     /locus_tag="VKU83_07590"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HLI93455.1"
                     /translation="MESYPSIFNDVIGPVMRGPSSSHCAASLRIGRLCRDLMEGHLNH
                     ILIEFDPNGSLATTHKSQGSDMGLFGGFLGWEADDERLPKSGELLDLEGIKVEIEVHD
                     IGASHPNTYKLTMTNREECHRLTAISTGGGMIQVIEIDGRKVSMSGDFYETLVYCEDP
                     QNVVAFLAESMELDELTWHDAGSFGVGFVEIKSGQFPDEGLCQEIAAMRGVKQIRKLS
                     PVMPVLSRKGLHVPFITCAEMLEYNRDRRLDLWELAVAYESERGNISPQAVFEKMRSI
                     VRIMKNAIHLGLKGTEYKDRILGSQSTQLKKKLEHKRLLGGDINNTITMYVSAIMEVK
                     SSMGVIVAAPTAGSCGALPGAVIGMAESLDLPEDETVKAMLAAGLIGVFVAAHATFAA
                     EVGGCMAETGSGAGMAAAGLVLLGKGTLQQSLAAASLALQNSLGLICDPIGNRVEAPC
                     LGRNVMAAANAVSCANMALSDYNHLIPLDEVIETMQSVGNEIHHTLRCTNLGGLSVTR
                     TAREIEQRLENQQKSFYKIC"
     gene            complement(3001..3300)
                     /locus_tag="VKU83_07595"
     CDS             complement(3001..3300)
                     /locus_tag="VKU83_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93456.1"
                     /translation="MGIRLRINVVYRNPTITETAEYARQCFVNLYPDKNKRGLIIVAF
                     MREELQMTEMQIKELMTDERTFRPENALKEDYAARLFLWIYVNHLSTPKYDRTGN"
BASE COUNT          962 a          784 c          925 g          926 t
ORIGIN      
        1 aaaaacggca gcgatatttc ctatccgctc ttcagcgccg aatttttttg catattcagc
       61 atctacaaac ctatagtcaa aaggttgagt cgggtcgtac ttccgcaaaa tacccgttat
      121 ttgtcccagt gccgcgcgca cactcattgt cggattgagc ttgacccacg agcataacga
      181 tttattaacg aaagccttca ttttgaattc ttggaaaggc tgttgtcatt aaaaagacgt
      241 atcttcattt tgcatacagt gaataaggat tacaagctcc ggtgacatca ttcatcatct
      301 tacagtcgtt gtctggctat aacttttgcg atctgcactt catttttcct gacatcattt
      361 ttaaaatagc attatgctaa aagtaatttt tttggcctct atcaattcgg tcagcctaaa
      421 aataccgttg ctcaaaaggc ctggaacaaa aggacgattc actggcagcg cagcaagagg
      481 caaggcaagc ggaggacgcg gcgggcgacg ccagcactaa cgcgaggatt ccgcaggcca
      541 gcagtgggcg cggtcccatt cggaaagact gtcggcatta attattaaat gaaattttga
      601 atgaataaaa aaggccccgt gatcgtcatc gaggacgacc atgatgatca ggaaattctt
      661 ggcgaaattt tcgaaaaact aggatatccg aacaaggtca tgttttttaa agatggcaat
      721 gtcacgcttg aattcctcaa ccagacggat gtccatcctt ttctaatcct atcggatata
      781 aacatgccgg ctataaatgg ttttgaactg cgcaataaga tatatacgaa tgaaatgtta
      841 caaacgaagt gcatacctta tctttttttc accacaagca gcaacagaaa ggaagttgag
      901 aaggcgtacg gattatcggt ccagggtttt tttgtgaagc caaattcatt caatgaattg
      961 gagagcacaa taaaaaaaat aatggaatat tggatagaat gcatcgcgcc aagccattat
     1021 gtggtggctt gaggtttccg ccctaaacga agtgaaaaat ccaccgcgtc atccatttcc
     1081 atgaagagta agggtggttc agccgaatat tcctaaaaat gttacttgag aagctatttt
     1141 gggggtacgc aagtttggtg gaatatacgg ccttacgtat atttagtagg ccgcgaaagg
     1201 ccagcttttc ccgtctttaa tcgcctacgt taaattatac atcatggaaa gctatcccag
     1261 tatttttaat gacgtcattg gaccggtcat gcgcgggcct tccagctccc attgtgccgc
     1321 ttcactccgg atcgggcggt tgtgccgcga tttgatggaa ggtcatttga atcatatatt
     1381 gatcgaattc gatcccaatg gttctctcgc caccacgcat aagagccagg gttctgatat
     1441 gggtttattc ggaggattcc tgggctggga ggcggatgac gagcgattgc caaagtcggg
     1501 agagctgctg gatctcgaag ggattaaggt agagattgaa gtccatgata tcggtgcgag
     1561 ccatccgaac acctataaat tgacgatgac caacagggag gaatgtcatc ggctcaccgc
     1621 aatctcaacc ggcggcggaa tgattcaggt aatagaaatt gacggaagaa aggtttccat
     1681 gagcggagat ttttatgaga cccttgttta ttgcgaagac ccgcaaaacg tagtggcatt
     1741 cctggcggaa tcgatggaac tggacgaact gacgtggcat gatgctggca gttttggcgt
     1801 cggctttgtc gagataaagt ccggacaatt tccggacgaa ggcctctgcc aggagattgc
     1861 agcgatgagg ggggtaaagc aaataaggaa gttgtcgccg gttatgccgg ttttatccag
     1921 aaaaggactc catgtaccgt tcataacctg cgcggaaatg ttggagtaca atcgcgacag
     1981 gcgcttggat ctttgggagt tggctgttgc ctacgaaagt gaaaggggca atatttctcc
     2041 acaagcggtt ttcgaaaaaa tgcggtcgat tgtccggatc atgaaaaatg ctatccatct
     2101 tggcctcaaa gggaccgaat ataaggatcg gattttgggc agccagtcca ctcaattgaa
     2161 aaaaaagctc gagcataagc gcctcctggg cggcgatata aacaatacga taaccatgta
     2221 cgtgtcagcc atcatggagg tgaaaagttc gatgggagtg atcgtggcgg ctcctaccgc
     2281 cgggtcgtgc ggggcgttgc cgggcgctgt gatcggaatg gcggaatccc tggatttgcc
     2341 ggaagatgaa accgtaaagg ctatgctggc cgcagggctg atcggcgttt ttgtagctgc
     2401 ccatgccacc tttgccgccg aagtaggcgg ctgtatggct gaaaccggat ctggggccgg
     2461 gatggcagcc gccgggttgg ttctgcttgg gaaaggtacc ttgcagcaat ccctggcggc
     2521 cgcatcgctg gcgttgcaaa actcgcttgg tttgatttgt gacccaattg gcaacagggt
     2581 ggaagcgccc tgcctgggga gaaatgtgat ggcggccgcc aatgcagttt cctgcgccaa
     2641 tatggcactc tccgactata accacctgat accgttggat gaagtaatag aaaccatgca
     2701 gagcgtcgga aacgaaatcc accacacgct gcggtgcacg aaccttggcg gtctttctgt
     2761 aaccaggacg gccagggaga tcgaacaaag actggaaaat cagcaaaaat cgttttacaa
     2821 gatttgttga atgatgcttc ttacccgcta gcataacgtc atacaaatta tagcgttcat
     2881 tttaaatttc cggatgggtc ccctaatagg tcaccgacga caatttgact tactttgatg
     2941 tgatttgaaa gagctatttc acgtttacca aggtacgaaa tacggaaaag agtactccgg
     3001 ctaattaccg gtccgatcat atttgggtgt agacaaatga ttgacataaa tccaaagaaa
     3061 cagtcgcgcg gcatagtcct ccttcaatgc gttttccggc ctgaaggtcc gctcatctgt
     3121 catcagctcc ttaatctgca tttccgtcat ttgcagttct tcccgcataa aggccacgat
     3181 aatcaatccc cgtttatttt tatcaggata gagatttaca aagcattggc gggcatattc
     3241 ggcagtctcg gtaattgtcg gattacgata cactacgttt attcgcagac ggatacccaa
     3301 cacgatatat cttgtaatga ttttgatgcc tagggtgcaa ggtcaatgca gcggctcctg
     3361 ctaggtaggc attgaagacc cggaggatat tgctggcgcg actggcttca tcgacggtaa
     3421 aggtgatcag gaaagggaat cgcttaatgg gtttgaagcg gacgttcttg taacggaagg
     3481 ctgaggcgtt gggctgttcc gcgatctcgg tgggtggccg atgaagcgtc agcacaggtt
     3541 gatggccgaa tatgtccgcc tcttgtcctt tttaattgaa aaccattggt atatctt
//
LOCUS       DATGKE010000439         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_360240, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000439 DATGKE010000000
VERSION     DATGKE010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360240"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VKU83_07600"
     CDS             <1..1535
                     /locus_tag="VKU83_07600"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93457.1"
                     /translation="VVLLAEDNADMRDYVGRLLSEQYRVITAKNGEEAFEIMQKVKPD
                     LLLSDVMMPKLDGFGLLQKVRLHPRLRTTPVILLSARAGEESKLEGLKAGADDYLVKP
                     FSARELIARVDANLRINAARHVVERELASFLMQAPFAIVILSGPDYVIDLVNDQSLPI
                     IQRSREEVLGRPAAEVAPEGLKRFKSFLDEILETGEPAVMNEQESRIDRNGTIDTAYL
                     TIAFQPLRRLDGHAERIMIVVEDVTDRVLARRRSEQYAALLEAEVKKRTQELNQLNAT
                     LRQSNEDLRQFAHVASHDLKEPVRKVKVFAGRLEDDPATTFSAKARSYLGKINSAASR
                     MLTMIEGVLHYSTINSTAQQTEAVDLNRVITAIESDLEVPIAEKKATVHYSGLPTVEG
                     AALLLYQLFYNLINNSLKFSRANEPCRIDITAAVENDQAVIILRDNGIGFDQEESERI
                     FDSFSRLNTRDKYEGTGLGLALCKKIAVRHGGDIEATGKKGIGSEFHIRLPLKGGIGR
                     VI"
     gene            1550..1993
                     /locus_tag="VKU83_07605"
     CDS             1550..1993
                     /locus_tag="VKU83_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93458.1"
                     /translation="MRSIYLIDDDMDDVELFRDALEEVAPSVSLRFANDAHEAVRNLA
                     ASAGALPDLVFLDISMPVLSGLQCLATLKKDDQLKGLPVIMYTTTSHEKEIRMARELG
                     ALAFITKPNDFRLLKRILAAVLDTPVERLSEELRKITDTGSGLQE"
     gene            2018..2671
                     /locus_tag="VKU83_07610"
     CDS             2018..2671
                     /locus_tag="VKU83_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HLI93459.1"
                     /translation="MKALYICLLVLPNILPRYGSAPGRTGVICPAADRNEWTLRREKD
                     GINVYSRRSDFSKFNDTRVDADLPGTIQQLAGILLDVDRYSDWVYATRTSRMVRKLNP
                     NEVIYYAEVGTPWPASNRDYYADMKISFNADGQSMTVVSVGMKDYQPEKKDLVRVPLS
                     RGYWTVTTTGRNKIHLQYILEIDPGGAVPAWILNNFASRAPLETFAKLKKKMEDLNR"
     gene            complement(2675..3286)
                     /locus_tag="VKU83_07615"
     CDS             complement(2675..3286)
                     /locus_tag="VKU83_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HLI93460.1"
                     /translation="MMLFEEAALFNEGVRRYTDFRLPDADLRLWEQFFSKQESDTFFR
                     QMKDNTPWKQRTRKMYDKMVLDPRLTAWYGGPEGLEWTESLLVVKAKVEKECGIMFDR
                     VLLNLYRDGNDSVAWHSDTLPADGKHRPIASVTFGDTRIFRIRHKTNKGFKTLDIPLT
                     HGSFLLMGETMQNHYEHHVPKTTRPVGPRINLTFRIGSPFQPE"
BASE COUNT          798 a          902 c          936 g          704 t
ORIGIN      
        1 atgtagtcct gctggccgaa gacaacgcgg atatgcggga ttatgtgggc cggcttctgt
       61 cagagcaata tcgggtcatc acggcaaaga acggggagga agcatttgaa ataatgcaga
      121 aggtgaaacc ggatctgctg ctctcggacg taatgatgcc aaagctggat gggttcggtt
      181 tgttgcaaaa ggtccggctg catccccgct tgcggacgac tccggtcatt ctgctgtccg
      241 cgcgcgccgg tgaagaatcg aagctggagg gtcttaaggc aggagcggac gactacctgg
      301 tgaagccctt ttcggcgcgg gaactcatcg ccagggtcga tgccaacctg cgcatcaacg
      361 ccgcccgcca tgtcgtggaa cgggagctcg ccagcttcct gatgcaggcg ccctttgcca
      421 ttgttatcct gagtggtccc gactatgtca tcgatctggt caacgaccag tccctgccga
      481 tcatccagcg cagccgcgaa gaagtactcg gtcgcccggc cgccgaggta gcgccggaag
      541 gcttaaagcg gttcaagtcc ttccttgatg agatccttga gaccggggag cccgcagtaa
      601 tgaacgaaca ggaaagccgc atagaccgca acggcacgat cgacacggct tacctgacaa
      661 ttgcttttca gccgctccga cgtttggacg gccatgcaga aaggatcatg atcgtcgttg
      721 aggatgtgac ggatagggtt ctggcaaggc gccgttccga gcagtatgcg gcgctgctgg
      781 aagcggaagt gaagaaacga acacaggagt tgaatcagct gaacgccacc ctccggcaat
      841 cgaatgaaga cctccggcag tttgcccatg tagccagcca cgatctgaag gagccggtac
      901 gcaaagtcaa ggtttttgcc ggccggctcg aagacgatcc ggccaccact ttctccgcca
      961 aggcaaggtc gtatcttgga aagatcaact cggccgccag ccgcatgctg accatgatcg
     1021 aaggggtgct gcactactcg accatcaatt cgacagcgca acagacggaa gcggtggacc
     1081 tgaaccgggt cattacggcg atcgagtcgg atctggaagt gcccattgcc gagaaaaaag
     1141 cgacggtgca ctattccggg ttgcccactg tcgagggagc cgcgctgctc ctgtatcagc
     1201 tgttctacaa cctgatcaac aattcgctca aattctcgag ggcgaacgaa ccgtgccgga
     1261 tcgacatcac tgcggcggta gaaaacgacc aggccgtgat cattttgaga gacaacggaa
     1321 tcggttttga tcaggaggaa tcggagcgta tttttgacag tttttcgcgc ctcaacaccc
     1381 gggacaaata tgaagggacc ggcctgggtt tggcgctctg caagaagatt gcggtgcgac
     1441 acggcggtga tatcgaagca acgggcaaga agggaatagg ttcagaattt cacatccggt
     1501 tgccgttgaa aggagggatt ggccgggtaa tataggctta aggaacgata tgcggagcat
     1561 ctacctcata gacgatgaca tggacgatgt ggaactattc agggacgccc tcgaagaggt
     1621 ggcgccatcc gtttccctgc ggttcgccaa cgacgcccat gaagccgtcc gcaacctggc
     1681 ggccagcgca ggagccctgc cggacctcgt gttcctcgac atcagcatgc ccgttttgag
     1741 tggcctgcag tgccttgcta cactcaaaaa ggatgaccag ctaaagggtt tgccggtcat
     1801 catgtacacc acgacttcac atgaaaagga gatcaggatg gcccgggaac tcggcgccct
     1861 cgcgtttatc accaaaccca atgacttccg gttgttgaag cgtatccttg ccgccgtgct
     1921 ggatacgccc gtcgagcgct tgtccgagga acttaggaag atcacggata cggggtccgg
     1981 gttgcaggaa tgagatttaa ttcgtatatt gaaatacatg aaggctctgt atatctgttt
     2041 gctggtcctc cccaatatcc tcccccgcta tgggtccgcg cccgggcgga ccggggtaat
     2101 ttgtcctgcc gccgaccgga acgaatggac tttgcgaagg gaaaaagacg gcattaatgt
     2161 ctacagccgc cgttcggact tctcgaaatt caacgatacc cgcgtcgacg cagaccttcc
     2221 cggcacgatc caacaactgg ccggcatcct ccttgacgtg gaccgttatt ccgactgggt
     2281 ttatgcaacg cggacttcaa ggatggtgag gaagctgaat ccgaacgaag tgatctatta
     2341 tgccgaagtc ggcaccccgt ggccggccag taaccgggat tattatgcgg acatgaagat
     2401 cagcttcaac gccgatggcc aaagcatgac cgtcgtatcc gtcggaatga aggattatca
     2461 gcccgaaaaa aaggacctgg tcagggtccc cctttcacgc ggctattgga ccgttacgac
     2521 tacagggcgc aacaagatac acctgcaata catcctcgag atcgatcccg gcggcgcggt
     2581 gcctgcgtgg atcctcaaca atttcgcctc ccgggccccc ctcgagacgt tcgccaaatt
     2641 gaaaaagaag atggaagacc tgaaccgcta ggcgctattc cggttgaaac ggcgacccaa
     2701 tgcgaaatgt taaatttatc ctcggaccta ccgggcgggt cgttttggga acgtgatgtt
     2761 cataatggtt ctgcatcgtt tctcccatca gcaggaagct accatgggtc aggggaatgt
     2821 ccagggtttt gaaaccctta ttcgtcttgt gacggatgcg aaagatccgc gtgtccccga
     2881 aagtcaccga cgcgataggc cggtgtttcc catcggcagg caaggtatcg ctatgccagg
     2941 caaccgagtc atttccatcc cggtacagat tcagcagcac gcgatcaaac ataattccgc
     3001 attccttttc cactttcgct ttgacaacga ggagcgattc ggtccattcc aatccttccg
     3061 gtccgccata ccaggccgta aggcgaggat caaggaccat tttatcgtac atttttctcg
     3121 tccgttgttt ccagggggta ttgtccttca tttgccgaaa gaatgtgtcc gactcctgtt
     3181 ttgaaaaaaa ttgctcccaa agccggagat cggcatcggg aagccggaaa tccgtataac
     3241 ggcggactcc ttcgttaaaa agggcggctt cttcaaacag catcatttga caaatatata
     3301 gggaaaaaac taaaaaaatt agcttttccg gcctgcaggc
//
LOCUS       DATGKE010000440         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_328508, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000440 DATGKE010000000
VERSION     DATGKE010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328508"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VKU83_07620"
     CDS             complement(<1..966)
                     /locus_tag="VKU83_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI93461.1"
                     /translation="MGKKLISLFAAFAFICLFSVPAFSQSAVTIKGTVRQATTKDRVS
                     AVSVTIKGTLIGTFTDDRGNFQLTTTQPLPVTLVFSSIGFETQEVAVSDAASPVDVSF
                     VPTSSLGREVVVSATRGAIRSLESPVTIERMGSTAAHEAPAPSFYDAVANMKEVDAVT
                     SSLLFKTMGTRGFNGSGNLRMNQLVDGMDNQAPGLNFSVANIVGLNELDVDHVELLPG
                     ASSALYGSGGMTGTLLMTSKDPFKYQGFSAQVKQGINHVGDPDNSGAKPYYNWTARFA
                     KAFNNRFAFKLTADYVLATDWMASDMRNYVPVTGQITGDTRALPSY"
     gene            1055..1984
                     /locus_tag="VKU83_07625"
     CDS             1055..1984
                     /locus_tag="VKU83_07625"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLI93462.1"
                     /translation="MRTLQFPHQTRFYRGKVRDVYTISDRWLVMIASNRISAFDVILP
                     RPIPFKGQVLNQIAAYMLKATADLCPNWLMDTPAPNVAVGRLCQPFRVEMVVRGNLTG
                     HAWRTYQSGKRELCGVPLPAGMKENDYFPSPIITPSTKAEVGHDEDISPAEIIRRGIV
                     GEKDYALLEQYSLALFQRGRDIASGRGLILVDTKYEFGRIGETVYLMDEIHTPDSSRY
                     FYAEGFDDRQARGERQLQLSKEFVREWLIANNFMGKQGQTVPEMTDEWVETISKRYIE
                     LYEKVIGEPFRPQELSDEEVYQRVLYSLGNLNG"
     gene            complement(1987..>2254)
                     /locus_tag="VKU83_07630"
     CDS             complement(1987..>2254)
                     /locus_tag="VKU83_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI93463.1"
                     /translation="IYLEARELRSTIEVSVQDTGMGISREGLERLTNRNYYSTRGTGG
                     EAGTGLGLMLCNDFLSRNGGRMNIQSELGKGSTFSFTLPKGRGE"
BASE COUNT          505 a          637 c          630 g          482 t
ORIGIN      
        1 atagctgggg agcgcacggg tatcgccggt gatctggccg gtgaccggca cataattccg
       61 catgtccgat gccatccagt ccgtcgccag gacataatcg gctgtcagtt tgaaggcgaa
      121 ccggttattg aatgccttgg cgaaccttgc cgtccagtta taatagggtt ttgcccccga
      181 gttatccgga tcacccacgt ggttgatccc ctgcttcacc tgcgcgctga atccctggta
      241 tttgaacgga tctttgctgg tcatcaggag ggttcccgtc atcccgccgg agccatacaa
      301 ggcggaagaa gctccgggca gcaactccac atggtccacg tccagttcgt tcagcccgac
      361 gatgtttgcc acggaaaaat tcaagcctgg cgcctggttg tccattccat cgaccagctg
      421 gttcatccgc aggttgccgc tgccattgaa gccgcgggta cccatcgttt tgaaaagcag
      481 ggaagaggtc acggcatcga cttccttcat gttggccacc gcatcgtaga aagacggcgc
      541 gggcgcttca tgcgcggccg tacttcccat ccgctcgatc gtcacgggcg actcgaggct
      601 ccggatggcg ccccgcgtgg cggaaaccac cacctccctg cccagcgaag aagtcggaac
      661 aaaggagacg tcgaccgggc tggcggcgtc gctgaccgcc acttcctgcg tttcgaagcc
      721 gatggaggaa aagaccagcg ttacgggaag cggttgcgta gtcgtcaact ggaaattacc
      781 gcggtcatcc gtaaatgtcc cgatcagcgt gccctttatc gttacggaaa cagcggaaac
      841 ccgatccttc gttgttgcct gacggacggt gcccttaatc gtgacagccg actgggaaaa
      901 ggccggaaca gagaagaggc aaatgaaggc gaaggcggcg aatagagata tcagtttctt
      961 tcccatagcg cgagttttgg tttagagaag cataacgtat tactaaaata cgtattgtaa
     1021 tgctgtcctc gaaatttaat ttatttttgc cgccatgaga acactgcaat ttccccatca
     1081 aaccaggttc tatcgcggga aagtaaggga cgtatacacg atatccgatc gctggctggt
     1141 catgatagcg tccaaccgca tctcggcatt tgacgtcatc cttccccgcc cgatcccttt
     1201 caaaggccag gttttgaacc agattgcagc ctatatgctc aaagctaccg ccgacctctg
     1261 cccgaactgg ctcatggaca cgcccgcgcc caatgttgcg gtcggccggc tttgccagcc
     1321 ttttcgcgtg gagatggtcg tgagaggcaa cctgacgggg catgcctggc ggacctacca
     1381 atccggcaaa cgggaacttt gcggggtgcc gcttccggcg ggaatgaaag agaacgacta
     1441 tttcccttcg cccatcatta ccccttcgac caaggcggag gtaggtcatg atgaagacat
     1501 ctcgccggcc gaaatcatcc ggaggggaat cgttggggaa aaggattacg ccctcctcga
     1561 gcaatatagc ctggccctct ttcaacgggg gcgcgacatc gcctccgggc ggggattgat
     1621 cctcgtcgac acgaaatatg aattcgggag gatcggagaa acggtctacc tgatggatga
     1681 gatccacacg cccgattcct cgcggtattt ctatgcggaa ggttttgatg accgccaggc
     1741 ccgcggtgag aggcaactcc agcttagcaa ggagtttgtc cgggaatggc tcattgcgaa
     1801 taattttatg ggaaagcaag gccagaccgt gccggaaatg accgatgagt gggtggaaac
     1861 gatcagtaag cgatatatag aattatacga gaaggtaatc ggcgagccgt tccgccccca
     1921 ggaactctct gacgaagaag tgtatcagcg cgtgctttat tcgctcggaa acctgaatgg
     1981 atagccttac tccccgcggc cctttggcag cgtaaacgaa aaagtgcttc ccttgccaag
     2041 ttcgctttga atattcatgc gcccgccgtt gcgcgagaga aaatcattgc acagcatcag
     2101 cccgagaccc gtaccggctt cgccgcccgt gccacgggtc gaataatagt tccggttcgt
     2161 caggcgctcg agcccctccc ggctgattcc catcccggta tcctgcacgg aaacctcgat
     2221 ggttgaccgc agttcgcggg cttccaggta aatg
//
LOCUS       DATGKE010000441         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1273824, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000441 DATGKE010000000
VERSION     DATGKE010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273824"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VKU83_07635"
     CDS             <1..1872
                     /locus_tag="VKU83_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93464.1"
                     /translation="ETYARILTVRGVLQDPPMNSTLKFGMLTPFDNLLNSNGRPIGPE
                     DWSTFADAAFFYIPDPSAVPAVVKGMLAWQGVQNKAREDWKVGGVRFVSMRQAASLSN
                     IIGNNYLYPRPDDAAAYAGFMLAFLIFLSCCLNFSNTTVSHAGIRLKEIGMRKVMGST
                     YRQLMIRLLAECSLIVLAAILLSMLLNSWWLPVFNSMFPGVQVEANYLNDRTLLLFLF
                     AIWLGSTLLAGFYPAFYLSRFNPTSIFRGTVKFGGSNLFSRLMLGLQLSVSIITVTAA
                     IAFTRNSAFQRDFDYGYNIESNMALILPDSTSYIAMKNKLTTMQGITGLAGSRNHLSF
                     DYKQSVAESEGFKKEVDFLEVGRDYPDVMGLKLTAGRGFDKGMGADYDNALLITEKTA
                     ALYGWSASTALGKQIRIDSAQLTVTGVLKDFHAATLFEPTPPIAIRLGRESRFHYLLI
                     SARPKDLAAVHEKVRSAWKSLFPLKPFNAFYQNQVKEQAYEINASTATIFGWFGIVSI
                     LLTATGLFALISLTTMKKMKEIALRKVVGASPRHILVLINKSYVLILAVSALLGSLAG
                     LALTRMLINMIFKINSGVGLDSLLWAVIVLFLIAAVTSGIKVWEAVRAHPVKLLRAE"
     gene            complement(1900..>2115)
                     /locus_tag="VKU83_07640"
     CDS             complement(1900..>2115)
                     /locus_tag="VKU83_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93465.1"
                     /translation="MGQSILLNEVSSSKYGGFDARTFSLGDFTATLGYKYPLKGRFSL
                     GAETKFFYSSRANDCNLALVLVGGYNF"
BASE COUNT          471 a          605 c          542 g          497 t
ORIGIN      
        1 gagacctatg cccgtatcct aacggtgcgt ggggtgctcc aagacccacc catgaactcg
       61 actcttaagt ttgggatgct gacgcccttc gacaacctgc tgaacagcaa tgggcgaccg
      121 attggtccgg aagactggag cacttttgcg gatgctgcct ttttttatat tcccgatccc
      181 tcggccgttc ccgccgtcgt aaaaggcatg ctggcctggc aaggcgtaca aaacaaggcc
      241 agggaggact ggaaggtcgg aggcgtccga tttgttagca tgaggcaagc tgcctccttg
      301 tcgaatatca tcggcaacaa ctatctctac ccccggcccg atgatgccgc cgcctatgcg
      361 gggtttatgc tggcctttct catcttcctt tcctgttgcc tgaacttctc caatacgaca
      421 gtgtcgcatg ccggaataag gttgaaagag atcgggatgc gaaaagtaat gggcagcacc
      481 tatcggcaat tgatgatccg gctgctcgcg gaatgcagtc tgatcgtcct cgctgccatc
      541 ctgctctcga tgctcttaaa ctcctggtgg ctgcctgtgt ttaacagcat gttcccgggt
      601 gtacaggtgg aggcgaacta cctgaatgac cgcaccctct tgctcttcct cttcgcgatc
      661 tggctggggt ccaccttact ggccggtttc tatcccgctt tttatctcag ccgtttcaat
      721 cctacgtcga tcttccgggg cacggtaaag ttcggcggca gcaacctgtt ctcccggctg
      781 atgttgggtt tgcaactgtc cgtctctatt atcacggtga cggcggccat cgcgtttacc
      841 cggaattcgg ccttccagcg ggacttcgac tatggataca acatcgaaag caatatggcg
      901 cttatcctcc ccgatagcac gtcctatatc gccatgaaga acaagttaac gaccatgcag
      961 gggataacgg gtctggccgg ctcccgcaac cacctcagtt tcgactacaa gcaatcggta
     1021 gcggaatccg agggttttaa aaaagaggta gattttctgg aagtggggcg ggactatccg
     1081 gacgtaatgg gcctgaaact gacggccggc cgcggtttcg ataaggggat gggcgcagat
     1141 tacgataacg cccttctcat cacggaaaag acagcggccc tttacggttg gagcgcttcc
     1201 acggccctcg gcaaacagat acggattgat agcgcccaac tcaccgtcac cggcgtattg
     1261 aaggacttcc acgccgccac cctcttcgaa cctaccccgc ccatcgccat ccgcctgggc
     1321 agggaaagcc ggtttcatta cctgttgatc agcgcccggc caaaggacct ggcagccgtg
     1381 catgaaaagg tgcgttcggc ctggaagtcc ttatttccac taaagccttt caacgccttt
     1441 tatcaaaacc aggtgaaaga acaggcctat gagataaacg caagcactgc taccattttc
     1501 gggtggtttg gcatcgtgag catcctgctc accgccaccg ggctgtttgc actgatctcg
     1561 ctcaccacga tgaagaagat gaaggagatc gccttacgga aagtagtggg ggccagtccg
     1621 cgacatatcc tggtcctgat caataaaagc tatgttctca tactcgccgt ttccgccctg
     1681 ctgggtagcc tcgccgggct ggcgctgacc cgaatgctga tcaatatgat cttcaagatc
     1741 aactcgggcg tcggcctcga ctccctgcta tgggctgtga tcgtactgtt cctcatcgcc
     1801 gcggtcacct ccggcatcaa agtctgggaa gcggtgcgcg cccatccggt caagttattg
     1861 cgagctgaat aagaaaggcc ttctttacaa gggggtgcct taaaaattat atccccccac
     1921 caatacgagc gccaaattgc aatcgtttgc ccgtgaagaa tagaaaaact ttgtctccgc
     1981 acccagggaa aatctccctt ttaatgggta tttatatcca agggttgccg taaagtcccc
     2041 caaagagaac gttcgcgcgt caaatccacc atacttcgaa gagcttacct catttaaaag
     2101 tatggactgc cccat
//
LOCUS       DATGKE010000442         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_2899, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000442 DATGKE010000000
VERSION     DATGKE010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..995
                     /locus_tag="VKU83_07645"
     CDS             30..995
                     /locus_tag="VKU83_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLI93466.1"
                     /translation="MKAVWITRKGGPEVLELRDTLAPVPAAGEVLIRVKAAGLNRSDI
                     YSRNSKAYGHDEPEIPGLEVSGTIETCGAGVTRWKPGDEVCALVTGGGYAEYVTVPGG
                     QCLPIPGGWSLEAAASLPETVHTVWHNVFRTAQLQKGENFLVHGGSSGIGTTAIQLAK
                     ALEIPVYTTAGTDEKCRWCESLGATCAANYKTEDFEAVFRNIGFDVILDMAGGDFTPK
                     NLRLLREDGRLVHINAIRGTNVTIDLARIMSRRLIITGSMLKPRDPAFKAALAADVEK
                     QVWPLIARGAIRPLIYAVFPLAEAAEAQALMESGTHYGKIILQTS"
     gene            complement(976..1452)
                     /locus_tag="VKU83_07650"
     CDS             complement(976..1452)
                     /locus_tag="VKU83_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLI93467.1"
                     /translation="MDKEHRYMSHLRWTGNKGAGTADYRAYDRDHVLSAPGKPEIPGS
                     SDPTFRGNPQRYNPEELLVSSLSSCHMLWYLHLAAINGVVVTAYEDNAEGTMVEAPDG
                     GGHFRLVVLHPIVTVIDPSMAEKAMALHHDAHKLCFIASSMNFPVEHQPQIRTSAE"
     gene            1482..>2196
                     /locus_tag="VKU83_07655"
     CDS             1482..>2196
                     /locus_tag="VKU83_07655"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF014199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HLI93468.1"
                     /translation="MVARLFLLMTMAFSSLAAFCQHPAFRVIPLGIKGGQDESNLSAY
                     LVAAEDASDYICLDAGTLHAGIRKAIENGVLPPPVNAVLRHSIKDYFISHPHLDHVAG
                     LILNSPEDTNKNIYALPSCMRVLQNNYFTWQSWANFGDQGEKPQLGKYHYVALEPGAE
                     ITAEHTTLAVRAFPLSHGNPYESTAFLVRNGDYSLLYLGDTGADTVERSDKLRQLWQT
                     VSPLIRQGRLKAIFIEVSFP"
BASE COUNT          479 a          677 c          595 g          445 t
ORIGIN      
        1 ctggccattg aaaaagacat tttgaacaga tgaaagcagt gtggataacc cgtaaaggcg
       61 gccccgaagt ccttgagctg cgcgataccc ttgcccccgt gcccgcggca ggcgaggtgc
      121 tgatcagggt taaggcagcc ggcctcaacc gcagcgatat ctattcccgc aacagcaaag
      181 catatggtca cgacgagccg gagatccccg gtctcgaagt atctggcacc attgaaacct
      241 gcggcgccgg cgtgacccgc tggaaacccg gcgatgaagt gtgcgccctc gtcaccggcg
      301 gcggctatgc cgaatatgtg acagtgcctg gcggccaatg cctcccgata cccggcggct
      361 ggagcctcga agccgccgcc tcgctccccg aaacggtcca taccgtatgg cacaacgtct
      421 ttcgcacagc acagctgcaa aaaggtgaga acttcttggt ccacggcggt agcagcggca
      481 tcggcacaac cgccatccag ctcgccaaag ccctcgagat accggtgtat accacggccg
      541 gcactgatga gaaatgccgc tggtgcgaat cgctcggcgc cacctgtgcc gccaactata
      601 aaacggaaga ttttgaagcc gtctttcgga atatcggctt cgatgtgatc ctcgatatgg
      661 ccggcgggga ctttaccccg aaaaacctcc ggctgctgcg cgaggacggc cggctcgttc
      721 acatcaacgc gatccgcggt acgaatgtca ctatcgatct cgcccgcatc atgagccggc
      781 ggttgatcat taccggtagc atgcttaaac cccgggatcc cgctttcaaa gccgccctgg
      841 ccgccgatgt agaaaagcag gtctggccgc tgatcgcccg cggcgccatc cggccgctca
      901 tctatgcggt ctttcccctc gccgaagccg cggaggccca ggccctgatg gaatcgggca
      961 cccactacgg caagatcatt ctgcagacgt cctgatctgc ggctggtgct ccaccggaaa
     1021 gttcatagag ctcgcgataa aacaaagctt atgcgcatcg tgatgcagag ccatcgcctt
     1081 ctctgccatg gaaggatcga tcaccgtcac aataggatgc agcacgacaa gcctgaaatg
     1141 gccgccgccg tcgggagcct cgaccattgt gccttcggca ttgtcctcat acgcggtgac
     1201 gacaacgcca tttatggcag ccaaatgcaa ataccagagc atatgacacg aagagagcga
     1261 gcttaccagc agttcttcgg gattgtagcg ctggggatta ccgcggaaag tgggatcgga
     1321 agagccgggg atctcgggct tgccgggtgc gctgaggaca tggtcgcggt cataagcccg
     1381 gtaatcggcc gtccccgctc ccttgttgcc cgtccagcgg agatggctca tatagcgatg
     1441 ttctttgtcc ataggaagta aagataccct atcttcactt tatggtcgcc cgtttgttct
     1501 tgctgatgac gatggccttt tccagcctcg ccgcattttg ccaacatcct gctttccgcg
     1561 ttatcccgct tggcataaaa ggaggccagg acgaaagtaa tctgtcggcc tacctggtgg
     1621 ctgcggaaga cgcctccgat tacatttgtc tcgatgccgg cacgctccat gcaggcatcc
     1681 gcaaagcgat cgaaaatggc gtccttcctc cgcctgtcaa cgccgtcctc cgtcattcca
     1741 tcaaggatta ttttatctcg catccccacc tggatcacgt cgccggtctg atcctgaatt
     1801 ctccggaaga tactaataag aatatctatg cgctgccatc gtgtatgcgt gtcctgcaga
     1861 ataactattt cacctggcaa agctgggcca atttcggcga ccagggagaa aaaccccaat
     1921 tgggaaaata tcattatgta gcgctggaac ccggcgccga aataaccgcg gagcacacca
     1981 cccttgccgt ccgcgccttt ccgctcagcc atgggaaccc atatgaaagc accgcctttc
     2041 tcgtccgcaa cggcgactat agcctgcttt atctgggaga tacgggcgcc gataccgtgg
     2101 agcgctccga taagctgcga cagctatggc agaccgttag cccgctgatc cggcagggcc
     2161 ggctcaaagc gatattcatc gaagtctcgt tccccg
//
LOCUS       DATGKE010000443         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1461171, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000443 DATGKE010000000
VERSION     DATGKE010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461171"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VKU83_07660"
     CDS             <1..260
                     /locus_tag="VKU83_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93469.1"
                     /translation="PAMRSLLSLFIGRERTATFSLRTYFMNTYQVYVYVLLLLYIPLV
                     LITMRGLVKTKIFPSPEIPITIPNVFKYLKLRLAGVSSYTP"
     gene            387..1361
                     /locus_tag="VKU83_07665"
     CDS             387..1361
                     /locus_tag="VKU83_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI93470.1"
                     /translation="MLKVGVFGVGHLGKFHLNNWKEIKTTELVGFYDPEDAAAAEATA
                     KYQLKRYDDPAQLMEDCDVVDIVAPTPAHYVLCQQAIRKGKHVFVEKPLANTMDEARE
                     LVKLVRESNVKLQVGHVERFNPAFLAVQSMPLNPLFIEVHRLAQFNPRGTEVSVILDL
                     MIHDIDIILSLVHSEVNSISASGVAVMTETPDIASVRIEFHNGCVANLTSSRISMKKM
                     RKMRLFQKDAYIGIDFLNKKSEIIRLKTAQDVDAFAFDITTPDGIRTIALSNPSVPEV
                     NAIRMELEEFTESIINNRPTKVSEVDGFRAMDIAHQILQKIKHNTINT"
     gene            1369..>2602
                     /locus_tag="VKU83_07670"
     CDS             1369..>2602
                     /locus_tag="VKU83_07670"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLI93471.1"
                     /translation="MSATKTSHTIPVFWYIISDYIAALLSSNIFHFSRRILLSEPIFV
                     NRHLLLTSRFWLGTITIPLGWLLLYALVGSYNSLYRKSRLNEAAATFVYCLIGCTIVF
                     FAIVINDPVKDYHYFYQTFLIFLSAHFILTLTGRLFVLGKVRRQVTSGRVVFNTLLVG
                     SDNIATHIYKSSKEGLRSSGYQYTGFIANPRQPSNGIADYLPELGDPDNIEATIDRHN
                     IRLVVLALERSDRREVETILEKLSHKDVDVKIIPSTLDILSGSVRTSNILGAVLSDIQ
                     TGLMPEWQQNIKRVTDIVVAAFTLIILSPLMIYAAIRTRLSSPGPVIYVQERIGYKGI
                     KFKIYKFRSMYRDAEKNGPALSSHDDQRITPWGRKMRKWRIDELPQLWNILRGEMSLV
                     GPRPEREYYVDQLYARTPY"
BASE COUNT          629 a          754 c          608 g          611 t
ORIGIN      
        1 cgcctgcaat gcgttcgctg ctttcgctct tcatcggccg ggagcgaacc gccacctttt
       61 ccctgcggac gtattttatg aatacctacc aggtctatgt ttacgtcctc ctgctgctct
      121 acattccctt ggtgctgatc accatgagag gattggtcaa aacgaagatc tttcccagtc
      181 cggagatccc gattaccatc ccaaatgttt tcaaatacct gaaacttcgg ctggccggcg
      241 tctcctccta tactccgtaa tgacatctcc gaagcgattg gttgtccatt gatcaattta
      301 atgtaggttt acggcggaaa ctcccgctcc gccgggcgga ttttggtttt ggatccatgg
      361 acatcatccg aaataaaaca tattaaatgc ttaaagtagg cgtttttggc gtcggtcacc
      421 tcggtaaatt tcatctgaat aactggaaag agatcaaaac aacggaactc gtcggatttt
      481 atgaccccga agacgcagcc gcggcggaag ccaccgcgaa atatcagctc aaacgttatg
      541 acgaccccgc gcaactgatg gaagactgtg atgtcgtgga tattgtcgcc cctaccccag
      601 cccattatgt actttgccag caggccatcc gtaaaggcaa acacgtcttt gtcgagaaac
      661 cattggccaa tacgatggat gaagcccgcg agctcgtaaa actcgtccgg gaatccaatg
      721 tcaagctcca ggtcggacat gtggaaagat tcaaccccgc cttcctggcc gtccagtcca
      781 tgccgctgaa ccccctgttc attgaagtgc atcgcctggc ccaattcaat ccgagaggca
      841 cggaagtgag cgtgatcctc gacctgatga tccatgacat agatatcatc ctgagtctcg
      901 ttcacagtga ggtcaacagc atctcggcaa gcggcgtggc ggtgatgacc gagacccccg
      961 atatcgcaag cgtccgcatc gagtttcaca acggctgcgt ggccaacctt acctcgagcc
     1021 gcatttcgat gaagaaaatg cgtaaaatgc gactctttca gaaggatgcc tatatcggca
     1081 ttgatttcct caacaaaaaa tcagagatta tccgcctcaa aacggcacag gacgtcgacg
     1141 cattcgcctt tgacatcacg acccctgacg gcattcgaac cattgccttg tcgaaccctt
     1201 ctgtcccgga ggtcaatgcg atcaggatgg aattggagga atttaccgaa tcgatcatca
     1261 acaacaggcc tacaaaagtc tcggaggtgg acggcttccg ggcaatggac atagcgcacc
     1321 agatcctgca aaagataaag cataacacca tcaacacctg actgctgaat gtccgcgaca
     1381 aagacctcac ataccatccc tgtattttgg tatatcatca gtgattatat cgcggccttg
     1441 ctgtcgtcca atatttttca tttcagccgc aggatcctcc tttccgaacc catcttcgtt
     1501 aatcgccact tgctgctgac ttcccgcttc tggttgggaa cgatcacgat cccccttggc
     1561 tggctgctgc tgtatgcgct ggtaggttcg tataattcgc tgtaccggaa atcgcgcctg
     1621 aacgaggcag ccgccacatt cgtctattgc ctgatcggct gtaccatcgt attcttcgcc
     1681 atcgttatca atgaccccgt gaaagactac cattatttct accagacctt tctgatcttt
     1741 ctttccgccc attttattct tacgctcacc ggcaggcttt ttgtcttggg aaaggtccgc
     1801 cggcaggtca ccagcggtcg cgttgtgttc aataccctgc tggtgggaag cgacaacatc
     1861 gcgacccata tctataaaag ctcgaaagaa gggctccgct cgtcgggtta ccaatataca
     1921 ggttttattg ccaaccccag gcagcccagc aacggcatag ccgactacct tccggaattg
     1981 ggtgaccctg acaacatcga agcaaccatt gaccggcata atatccggct ggtcgttctt
     2041 gcgctcgaac gttcagaccg cagggaagtg gagacgatcc tcgaaaagct gagccataag
     2101 gacgtcgacg ttaagatcat ccccagcacc ctggacatcc tttccggatc tgtccggacc
     2161 agcaacattt taggcgccgt tctgtcggat atccaaaccg ggctgatgcc ggagtggcaa
     2221 caaaacatca agcgggtaac cgatatcgtc gtcgcggcat tcaccctcat catcctttcg
     2281 ccgctgatga tctacgcggc gatacgtacc cgcctctcct ccccaggccc ggttatctac
     2341 gtccaggaac gcatcgggta taaaggcatc aagttcaaga tctataaatt ccggtccatg
     2401 taccgggatg ccgagaagaa tgggccggcc ctctcctctc atgacgacca acgaatcacc
     2461 ccctggggca ggaaaatgcg caaatggcgc atcgacgagc tacctcagct ctggaacatc
     2521 ctgcgcggcg aaatgagcct tgtcggccca cgacccgaac gggaatatta tgtcgaccaa
     2581 ttgtacgcgc ggacgcccta tt
//
LOCUS       DATGKE010000444         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_34511, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000444 DATGKE010000000
VERSION     DATGKE010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34511"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VKU83_07675"
     CDS             <1..1034
                     /locus_tag="VKU83_07675"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93472.1"
                     /translation="QVVTQMGDQGASCDGMRTLREWFEAGVIGDVEKVFCWTNRPVWP
                     QGISWPAKGATVPKELNWDLWLGTAKETYYIDNLVPFNWRGWWEFGTGALGDMGCHII
                     GPPFKLLKLGYPTSISGSASTVYSGIFKEGIYPQSGPVSSWVRFLFNGPGGREIELHW
                     MDGGIAPERPKELDPSVDMNEALADWPGENDYEGGTLFIGTKGKASCGWGGSHPRLLP
                     LSKTDEVHVPQKYPRVEGGMDGHWWQWVDAAIAGYGKMEVDSPFEGYAGPLTETVLLG
                     NLLLRSFNIQQKIERNDPVYGAMGGYKFPGRYITYQWDGANMRITNFDAANQFVRREY
                     RQGWGEVKL"
     gene            complement(1075..1779)
                     /locus_tag="VKU83_07680"
     CDS             complement(1075..1779)
                     /locus_tag="VKU83_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93473.1"
                     /translation="MLRCLAVDDEPLALQLLTDYIRKVPFLDLVATATDAFEAARVLQ
                     EKQVDLLFIDIQMPGLTGLQFIQSLAKRPMVIIITAYKKFAPEGFDLDVVDYLVKPVG
                     MDRFLKACNKAQELHDLRTSKTTVADAEFFFVNVDYSLVKVVFSDIVWVEGSGDYVKV
                     HLKSAPKPLLIRTTAKTLESELPPDKFLRIHKSYIVSKAAITAVRKNSVFIGELELPV
                     GETYRDILRQLTGRDL"
     gene            complement(1779..2819)
                     /locus_tag="VKU83_07685"
     CDS             complement(1779..2819)
                     /locus_tag="VKU83_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI93474.1"
                     /translation="MKTNWYNSRIIKVLTHAAVWAIVLSLPYLLDTHHGMGPRRNNRF
                     DQGFFYLNFVTSVLWIAPFYLNAYWLTPAYFNRRRYLPYIAMLVLLFAILMGIHFTFF
                     RFVFDLPHFSLKGATWFLLPPYILVVAVSATFRVVSDKLEADQLAKERHEENLKTELS
                     FLRSQINPHFIFNILNNLVALEQMKSPELGPTILKLSALMQYMLYETDEERVSLAKEV
                     EYLQSYIDLQKQRFGDKVPVSVSLEAAPGFYEIEPMLLIPFVENAFKHGVGMIDHPAI
                     SIELAVRDKVLHFSVRNRYNQASGEVKDKGSGIGLGNVKRRLKLLYGNQHVLAINRSN
                     GWFEVTLQLNLH"
     gene            complement(2881..>4164)
                     /locus_tag="VKU83_07690"
     CDS             complement(2881..>4164)
                     /locus_tag="VKU83_07690"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HLI93475.1"
                     /translation="YFAAGSGSSLTGSELQKIAGSGNDHNVILQTDFSDPVTSRTTIE
                     AGLRAALQSRLNINNNYTYNPDSAAYELIPSAASNYKSQSNVYAAYFTVSSRINDFTY
                     KLGLRAESSNYHGTLLNSGQAFSNQYPVSLFPSIFLGQKLGNNQELQLSYTRRVNRPN
                     FFQLVPYTDSSNKLNITRGNPNLVPEFTQSVELTWLKTFPGNSMLMASVYYRYTDHLI
                     TGFIQADTNAATGTTTLINTFINAESSRSTGAELTGQVSLTRWWDLSSNVNLYQSRIN
                     VGSTDGVAQQALWSWFGKLNTSFRLPSGFSMQLSGMYQSKTNLPVNTTGNQPGPPNMQ
                     NQSASQGYIRPYYEADIAIKKTFLANKLAMALSFSDIFRSRHQDQYTYSNYFTQEYDR
                     LRDPQMLRLNLTYSFGKVDVSLFKRKNNNIQTEEQ"
BASE COUNT          944 a          997 c         1265 g          958 t
ORIGIN      
        1 accaggtcgt cacgcaaatg ggcgatcagg gcgcatcctg cgatggaatg cgaacattgc
       61 gggaatggtt cgaggctggt gtgattggcg atgtcgagaa ggtattttgc tggaccaacc
      121 ggcccgtttg gccacagggc atttcgtggc cggcaaaggg tgcaacggtg ccgaaggaac
      181 tgaactggga cctttggctt ggcaccgcga aggaaactta ttatatcgac aatcttgtcc
      241 cgtttaactg gcgcggatgg tgggagtttg gaacaggcgc gctgggtgac atgggctgcc
      301 atatcatcgg accgcctttc aagctgctga agcttggcta cccgacttcc atatccggca
      361 gcgccagtac agtctatagc ggaattttca aggaaggcat ctacccgcaa agcgggccgg
      421 tctcgagttg ggtgcgattc ctattcaacg gcccgggcgg ccgggaaatc gagctgcact
      481 ggatggacgg tggaattgcc cccgaaaggc cgaaggaatt ggatccatcg gtggacatga
      541 atgaggcgct ggcagactgg ccaggggaaa acgattatga aggcgggacg ctatttatcg
      601 gaacaaaggg caaggcatcc tgtggatggg gaggcagcca cccccggctg ctgccgctgt
      661 cgaagaccga cgaagtgcat gtcccgcaaa aatacccgcg tgtcgaaggc gggatggacg
      721 gccactggtg gcagtgggtg gacgcagcca tcgccggtta tggaaaaatg gaggtggatt
      781 cgccattcga aggctatgcg gggccgctaa ccgaaacggt gctgctgggt aacctgctgc
      841 tgcggagctt caatatccag cagaagatcg agcgaaatga tccggtatat ggagcgatgg
      901 gcgggtataa atttccgggg cggtatatca cctaccagtg ggatggagcg aatatgcgga
      961 tcaccaattt tgacgcagcc aatcaattcg tcaggcggga atatcgccag ggttggggtg
     1021 aagtgaaatt gtagcggacc gccttcgtca gagctacggc gtggccgggg cgtactacag
     1081 gtccctgccg gtcagttgtc gcaggatatc ccgataggtt tcgccgaccg gcaattccag
     1141 ttcgccaatg aacacgctgt tcttgcgcac ggccgtgata gcggccttcg atacgatata
     1201 ggatttgtgg atccgcagga atttgtcggg gggcagttcc gattcaagcg ttttggctgt
     1261 cgtccggatc agcaatggct tgggcgcgct tttcaggtgg acctttacat agtcacctga
     1321 gccttcgacc caaacgatat ccgaaaaaac tacttttacc agactgtaat ctacgttgac
     1381 aaagaaaaat tcggcgtccg caacggtcgt cttgctcgtt cgcaaatcgt gcagctcctg
     1441 cgccttgtta caggccttca ggaagcggtc cattccgacc ggctttacga gatagtccac
     1501 tacgtccagg tcgaagccct ccggcgcgaa ctttttatat gcggtgataa taataaccat
     1561 cggccgtttg gccaggcttt ggatgaactg gaggccggtc aggccgggca tctggatatc
     1621 gataaaaaga aggtctacct gcttttcctg cagaacgcgg gcggcttcga acgcatcggt
     1681 cgccgtcgcg acgagatcca gaaagggcac cttgcgaata tagtcagtca gaagctgcag
     1741 tgccaggggc tcgtcatcga cagcaagaca tcgtagcatc agtgtagatt tagttgcagg
     1801 gttacttcaa accagccgtt gctgcggttg atcgcgagca catgctggtt cccatacagc
     1861 agcttcaacc ggcgtttgac attgccgagg ccgatgccgg accctttgtc cttcacctcg
     1921 ccggatgcct ggttatacct gttccttacc gagaaatgaa ggaccttgtc ccggacggcc
     1981 aattcaatgc tgatcgccgg gtggtcgatc atgccgactc cgtgtttgaa cgcattttca
     2041 acgaaaggga tcagcagcat cggttcgatc tcatagaatc ccggcgcggc ttccaaagat
     2101 acggagaccg gcaccttgtc gccaaaccgc tgcttttgaa ggtctatgta ggactggagg
     2161 tattcgacct ccttggcaag ggacacacgc tcttcatccg tttcatacaa catatactgc
     2221 atcagggcag acagcttcag gatagtgggc cccagctccg gcgacttcat ttgttcgagg
     2281 gcgacgaggt tgttcaggat attgaagatg aaatgcggat tgatctgcga ccggagaaag
     2341 gaaagttcgg ttttcagatt ctcttcgtgt cgctccttcg ccagttggtc tgcctcaagc
     2401 ttatcgctga caacgcggaa ggtcgcgctg acggcaacga ccaggatata cgggggtaga
     2461 aggaaccagg tcgccccctt caggctgaag tgcggaagat cgaatacgaa acggaagaac
     2521 gtaaaatgta tgcccatcag aatcgcgaac aacagcacca gcatggcgat ataaggcaag
     2581 tagcggcggc ggttgaagta ggccggcgta agccaataag cgttcaggta aaagggagcg
     2641 atccaaagga cactcgtaac gaaatttaga taaaaaaagc cctggtcaaa gcggttgtta
     2701 cgccgcgggc ccatcccgtg atgcgtgtcc agaaggtagg gaagcgaaag gacgatggcc
     2761 cagaccgctg catgcgtcag cactttgatg atcctgctat tgtaccagtt tgttttcatg
     2821 gttgtccggg tggggccgtg taaatataat gttttattta aagggtttga tccaaaagga
     2881 ctattgttct tccgtctgga tattgttgtt tttccgcttg aagagactga catcgacttt
     2941 tccaaaacta taggtaaggt tgagccggag catttgcggg tccctcagcc ggtcatattc
     3001 ctgcgtgaaa taattgctgt atgtgtattg atcctggtgg cggctgcgga agatgtcgct
     3061 aaaactcagg gccatggcca gtttgtttgc caggaaggtc tttttgattg caatatcggc
     3121 ttcgtaataa gggcggatgt agccctgtga ggcactttgg ttctgcatgt tcgggggccc
     3181 gggctggtta ccggtcgtgt tcaccgggag gttggtcttc gactgataca tccccgaaag
     3241 ctgcatactg aaaccggaag gaaggcggaa actggtattg agcttgccaa accagctcca
     3301 gagggcttgc tgcgcgacgc catcggtcga gccgacattg atcctggatt gatacaggtt
     3361 aacattggat gacaggtccc accaccgggt aagggacacc tggcccgtca attcggcgcc
     3421 cgtcgaacga ctcgactcgg cattgatgaa ggtgttgatg agcgtagtcg tgcccgttgc
     3481 cgcattggta tcggcctgta taaagccggt gatcaggtga tcggtgtacc tgtagtagac
     3541 cgaggccatc agcatgctgt tgccggggaa tgttttcagc caggtaagtt cgaccgactg
     3601 ggtgaactcc ggcaccaggt tcggatttcc gcgcgttatg ttgagcttat tcgatgagtc
     3661 ggtataaggg acgagttgga agaaattggg ccggttgacg cggcgggtat agctcagctg
     3721 caattcctgg ttgttgccga gcttttggcc gagaaagatg gatgggaaca gactgaccgg
     3781 gtattggttg ctgaaagcct gaccggaatt caagagtgtg ccatggtaat tcgagctctc
     3841 tgcccgaagg ccgagcttgt aggtgaagtc attgatccgg ctggatacgg taaaataagc
     3901 ggcgtagaca ttgctttggc tcttgtagtt gctggcggcc gaggggatca gctcataggc
     3961 tgcagagtcg ggattatagg tatagttgtt attgatgttc aggcggcttt gcaaagcggc
     4021 gcgtagtccc gcttcgatgg tggtcctgga cgtaaccgga tcggaaaaat cggtttgcag
     4081 gatgacattg tgatcgttgc ccgagccggc tatcttctga agttccgacc cggtaagtga
     4141 agagccgctg ccggccgcaa aata
//
LOCUS       DATGKE010000445         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_612017, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000445 DATGKE010000000
VERSION     DATGKE010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612017"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VKU83_07695"
     CDS             <1..655
                     /locus_tag="VKU83_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLI93476.1"
                     /translation="AVSNPRGNDQRAFVLPDGSKVLLNAGSVLKYPPRFMSSDRQVEL
                     TGDAFFDVKPNPKSPFRVQIRNGEVEVLGTYFEIMAYGDEQASRTTLIAGALKVTSGH
                     QTEVLRPGDQAEISYPSPGVEAGLIVRSGIDIKATLDWRDGIYDVNGVDLKTVLQVLA
                     RRYDVTVEYQPNIQNPSINGNFDLHKSLDILLQGLNASLPGNIHIKAKQKTLTVTAN"
     gene            918..>3125
                     /locus_tag="VKU83_07700"
     CDS             918..>3125
                     /locus_tag="VKU83_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLI93477.1"
                     /translation="MDDLRAIAGITYVGMADLTKQGHPVTFSVRNATLKEVLDLCFKG
                     QPFGYKQVAGTIAIVPLEQPAEGTAIYGRVINSNNEPVAGVTVSINGSYRNIVATNDS
                     GEFRIAAPGPACVLTFTSVNYESQEVKGVRGRRLVVQLKDRVNELSDVVVVHTGFQDV
                     HKRMTTGSFDQIDNELVTRRVGPNILDRIDGISSGVLFNTNVVTGTNQSTVAIRGRST
                     IFSNPNPLIVVDNFPYTGDVNNINPDDVESITILKDAAAAAIWGALSGNGVIVITTKK
                     GRLNQKPKLVFNSSATVGGKPDLYYLPVLSSGDYINVEEYLFRNGYYDQAYKNGFHSA
                     LTPVVNLLYQDSAGMAAQIDALRAQDDRPGLSKYFYRPSLNSQVALNLSGGGEHNQYF
                     LSAGYDRDLANLVRNDYSRVTLDGANTYFLLSGRLELNTAMAFTASTTNNDNIGATGV
                     SYPYAQVADANGNPLPVAFGLNEPYVDTAGGGLLLDWHYRPLQEMRNADNPTRVQDFR
                     INAGVRYNVCRGLDIRAVYQYSHGYSDNPDYQSEQMYATRNLINEFTAITDGAVNYAV
                     PPGGILTENTTRYSANNVRLQADYVDTLSKRSVVNLLAGSEVRDIEGGSTTLQLYGYD
                     PAQEVGIPVDYVDFFPQYSSGTTAKIPYNDQKTATSERYLSYYGTGIFNYLGRYMLSA
                     SIRRDESNLFGVNENQKGVPLGSLGLAWQLSKEKFYHSDWLPFLKLRVTDGYNG"
BASE COUNT          776 a          769 c          871 g          709 t
ORIGIN      
        1 ggccgttagt aacccaaggg gtaacgatca gcgggccttc gttctgccgg atggcagcaa
       61 agtattattg aatgccgggt cagtgctgaa gtatccgccg cgattcatga gtagcgaccg
      121 gcaggtggaa cttacaggag atgccttttt tgacgtgaag ccgaatccaa agtcgccttt
      181 ccgggtgcag atcaggaacg gggaagtgga agtcctgggt acctatttcg agatcatggc
      241 ttatggcgac gagcaagcaa gccggaccac gctgatagcg ggagcattga aagtcacgag
      301 tggccaccag acggaagttc tgcggccggg tgatcaggcg gagataagtt acccctcacc
      361 cggagtagag gctggtctca tcgtgcgttc gggcatcgat attaaggcca cgcttgactg
      421 gagagatggc atttatgatg tcaatggcgt ggatttgaag acggtcctcc aggttcttgc
      481 gagaagatac gacgtgacgg tcgaatacca gccgaatatt cagaacccgt cgatcaacgg
      541 gaactttgat cttcacaaga gcctggatat tttgctgcaa ggattaaatg ccagtctgcc
      601 ggggaatatt catattaaag ccaaacagaa aacgctgaca gttaccgcca actgaccata
      661 ttattgtcca tagccattga accaagtcca aaaacagtta cagctgcaat tttgggtaga
      721 caaataagta tttattttat gtttactcat gatttgcagg ctgtaacttt attgcaccaa
      781 atcaatgctt gatgggccct tcgaaactgg ctgtctcctt tgtaaccctc ttcttattac
      841 tacgatgccc gtctggcgct tgtcagtcca ccagcaggat ctcattgacg gaaaaaaatg
      901 cgccattctc aaaagtcatg gatgatttgc gagcgatcgc cggaatcacc tacgtcggaa
      961 tggctgacct cacaaagcag ggccatccgg tgactttctc cgtccgaaat gccacgttaa
     1021 aagaggtatt ggacctttgt ttcaaggggc aacccttcgg ctataaacag gtagccggaa
     1081 cgattgcgat cgtgccgctt gagcaacccg ccgaagggac cgccatttat ggccgcgtga
     1141 tcaattcgaa taatgagccg gtcgcgggag ttaccgtatc aataaatggc tcttatcgga
     1201 atattgtggc aaccaatgat agcggcgaat tccggatcgc tgcaccgggc ccggcatgcg
     1261 tcctgacgtt cactagcgtc aactatgaat cccaggaggt caaaggggtt cgcgggaggc
     1321 gcctggtggt tcaattaaag gacagggtga atgaattgag tgacgtcgtc gtcgtacata
     1381 cgggattcca ggacgtgcac aaaaggatga ctacggggtc ttttgaccag atcgacaacg
     1441 aattagtgac ccggcgcgtc ggacccaata tccttgaccg gatcgacggc atcagcagcg
     1501 gggtgctgtt caacacgaac gtcgtaacgg gaaccaatca gtctaccgtg gccatccgtg
     1561 ggcgcagtac gatcttcagc aaccccaacc cgctgatcgt ggtggacaat ttcccctata
     1621 ccggcgatgt gaataatatc aacccggatg atgtggaaag cattaccatt ctgaaggatg
     1681 cggctgctgc tgcgatctgg ggagcgctct ccgggaatgg ggtgatcgtc attactacca
     1741 aaaaaggcag gctaaaccaa aaaccgaaac tggtattcaa ctcgagtgct acagtgggcg
     1801 ggaagccgga tttgtactat ttgccggtac tgtcttcagg cgattacata aatgtggagg
     1861 aatatctgtt tcggaatggg tattacgatc aggcctataa gaatggattt cacagcgccc
     1921 tcacaccggt agtcaatttg ctatatcagg attcagcggg aatggcggct cagatcgacg
     1981 cactgcgggc ccaggacgac cgccccggac tttcgaagta tttttaccgg ccgagcttga
     2041 acagccaggt cgccctgaat ctgagcgggg gcggcgagca taaccaatat ttcctgtctg
     2101 ccggttacga tcgggatctc gccaacctgg tccggaacga ttatagccgg gtaacgctcg
     2161 acggagccaa tacctatttc ctgctgagcg ggcggctgga actgaatacc gcgatggcat
     2221 ttacggcgag cacaaccaat aacgataaca tcggcgccac cggtgtcagc tatccttacg
     2281 cgcaggttgc cgatgcaaat gggaatccgc tgccggtcgc tttcgggttg aatgaaccgt
     2341 acgtcgacac cgccggcggc ggcttgcttc tggattggca ttaccgcccc ttgcaggaaa
     2401 tgcggaatgc ggataatccc acaagggtgc aggatttccg gatcaacgca ggggtccgct
     2461 ataatgtctg tcgagggctg gatatccggg ccgtttacca gtattcccac ggctattccg
     2521 acaacccgga ctatcaaagc gagcagatgt acgccacacg gaatctgatc aatgagttca
     2581 cggcaatcac tgatggagca gttaattatg cagtgccgcc gggcggaatt ctgactgaaa
     2641 atacgacgag gtattcggcc aataacgttc gcttgcaggc ggactacgtg gacacgttgt
     2701 caaagaggag cgtagtcaat ctgcttgcgg ggtcggaagt ccgggatatc gaagggggca
     2761 gcacgacact tcagttgtac ggctatgatc cggcgcagga agtcggcata cccgtcgatt
     2821 atgtcgattt tttcccgcag tattccagcg ggacgacggc aaaaattccg tataacgacc
     2881 agaagacggc gacatccgag cgatatcttt cttattatgg gaccggaata ttcaattatt
     2941 tgggccggta tatgctgtcg gcgagtattc gacgtgacga atcaaacctt tttggcgtga
     3001 acgagaacca gaagggcgtc ccgctgggat cgctgggtct ggcgtggcaa ttatcaaagg
     3061 aaaagtttta tcattccgac tggcttccgt tcctgaagct gcgcgtcacc gatggttaca
     3121 acggc
//
LOCUS       DATGKE010000446         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_418155, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000446 DATGKE010000000
VERSION     DATGKE010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418155"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VKU83_07705"
     CDS             complement(<1..296)
                     /locus_tag="VKU83_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93478.1"
                     /translation="MKKQPLNTFTFLFILALCLFATIVFAAPAPLTVDHTVLARKMSR
                     SAAQRAEKRMILATATSIYDEMSLEDSGLSRKAFEYAWTGFYKLRKRGLLKRAN"
     gene            524..1270
                     /locus_tag="VKU83_07710"
     CDS             524..1270
                     /locus_tag="VKU83_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93479.1"
                     /translation="MRALFFRIYDIYQFTIQFWKEVFIPPYEIKEVFNQFFQVGIRSL
                     SIISLTGFITGVVFTKQSRSSLAEFGATSWLPSLMAIALLRALAPLVTALIVAGKVGS
                     NIGAEIGSMRVTEQIDAMEVSSTNPFKFLVVTRVLATTFIVPVLAIYMGGIGLLGAFF
                     NVLRNEQTSFTSFFQEAFARITFLDLFMLVFKSAVYGFTIGMTGCFTGYSAQQGTVGV
                     GRAANTAVVASMFLIFIEEMVIVTVVNSYR"
     gene            1267..2046
                     /locus_tag="VKU83_07715"
     CDS             1267..2046
                     /locus_tag="VKU83_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI93480.1"
                     /translation="MMAGEKVVISLKNVRKSFGSLQVLRGVDLEVGEGQNVVVLGRSG
                     SGKSVLIKIIAGLLHPDSGTVRVLGQEVDQLDLRSLRQLRTKICFSFQGSALYDGMTV
                     RANLEFPLVRNKRNLSSKEVDQAVKKVLEDVGLSQTIDQMPAELSGGQKKRIGIARTL
                     ILKPQIMLYDEPTAGLDPVTSVDINELINEVQQQYNTSSVIITHDLTCARATGDRVIM
                     LSDGCFLRQGTFDEVFDTDDPKVKAFYNYNFIETTHDRQQQ"
BASE COUNT          487 a          532 c          595 g          499 t
ORIGIN      
        1 atattcgcgc gcttcagtaa cccccgcttg cgtaatttat aaaaaccggt ccaggcgtat
       61 tcaaaggcct tgcggctgag accggagtcc tcaagactca tttcgtcata tatgctggtg
      121 gcggtagcaa ggatcatccg cttttcggcg cgttgggccg cagatctgga cattttcctg
      181 gccaggaccg tgtgatcgac ggtcagcggc gccggagcgg cgaatacgat agtggcgaag
      241 agacacagcg cgagtatgaa taggaatgtg aaggtgttga ggggctgctt cttcataaaa
      301 acggatttga atgataatag ccaactggca taccaaaata taataaattg actttcaata
      361 gaataatccg ttattcgcct tagacttttt ccagaaaccg gacagctttg ccaattcttg
      421 gaaaaagtcc tgtaaccgga atatgtcgtt tgtagaaaat aggccccagg accgtgccgc
      481 gggccgtcaa attcactggc atgcaatttt acccgctaac cggatgaggg cattgttttt
      541 ccggatatat gatatctacc agttcacgat tcagttctgg aaagaggtct tcatcccgcc
      601 ttatgaaata aaagaagtat ttaaccaatt tttccaggta ggcatccggt cgctgtcgat
      661 catctcgctc actggtttta ttaccggcgt tgtctttacc aaacaatccc ggtcttcgct
      721 ggccgagttc ggcgccactt cctggctgcc ctcattaatg gccatcgccc tgctccgggc
      781 actggctccg ctggtgacgg cgctgatcgt tgccggcaag gttggtagca atatcggcgc
      841 tgagatcggt tcgatgcggg tgaccgaaca gatcgacgcg atggaggtct cgtccacgaa
      901 tccttttaaa tttctggtgg tgacacgcgt actggccacg acgttcatcg tgccggtgct
      961 ggcgatctat atgggcggta tcggattact gggcgctttt ttcaacgtct tgcggaacga
     1021 gcagacgagt ttcacctcgt tcttccagga ggcgttcgcg cgcattacct tcctggattt
     1081 gttcatgttg gtgttcaagt cggccgttta tgggtttacg atcggaatga cgggatgttt
     1141 taccgggtac agcgcccagc aggggacggt aggcgtcggg cgcgcggcca acacggcggt
     1201 agtggcttca atgttcctga tcttcatcga agagatggtg atcgtcaccg tggtcaacag
     1261 ctatagatga tggcaggcga aaaagtggtc atatcgttga agaacgtccg aaaatcattc
     1321 ggttcgctgc aagtgctgag gggggtggac ctggaagtcg gggaaggaca gaatgtcgtc
     1381 gtgctgggcc gctcgggcag tggaaaatcg gtgctgatca agatcatcgc cggcctgctg
     1441 catccggact ccggcacggt gcgggtcctg ggccaggagg tagatcagct cgaccttcgg
     1501 tccctgcggc agctgcgaac aaagatctgc ttttccttcc agggaagcgc gctgtacgat
     1561 gggatgacgg tgagggccaa cctggagttc cccctcgtcc ggaacaaacg gaacctcagt
     1621 tcgaaggaag tcgaccaggc ggtgaaaaaa gtgctcgagg acgtaggcct gtctcagacc
     1681 atcgatcaga tgccggcgga attatccggg ggacagaaga aaaggattgg cattgcccgt
     1741 accctgatac tgaagcctca gatcatgtta tacgacgagc caacggccgg cctcgacccg
     1801 gtcacgagcg tcgacatcaa cgaactgatc aacgaagtgc agcagcagta caatacgagc
     1861 tccgtgatca ttacgcacga cctgacctgt gcccgcgcaa ccggcgaccg ggtgatcatg
     1921 ctgtccgacg gatgcttttt gcgccaggga acctttgacg aagtgtttga caccgacgat
     1981 ccgaaagtga aggcctttta taactataat tttatcgaaa ccactcatga ccgacaacaa
     2041 caataaccgt aaaaaactcg ccgtgggcgc ttttattctt atcggcctcg ccattttgct
     2101 ggccggggca ata
//
LOCUS       DATGKE010000447         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_706776, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000447 DATGKE010000000
VERSION     DATGKE010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706776"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VKU83_07720"
     CDS             <1..308
                     /locus_tag="VKU83_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93481.1"
                     /translation="AAEQRTREIGVRKVLGAGIATIVRMLSMDFIKLVLLSIVIASPL
                     AWLAMHNWLEGFAYRIGISVWIFIIAGLIALLIAVVTVTFQTVKAAVANPVDSLKTE"
     gene            complement(320..1255)
                     /gene="fmt"
                     /locus_tag="VKU83_07725"
     CDS             complement(320..1255)
                     /gene="fmt"
                     /locus_tag="VKU83_07725"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLI93482.1"
                     /translation="MQTRPRIVFMGTPDFAVASLDAIHRAGYPIAGVITAPDKPAGRG
                     MQLMESAVKKFAREHDLRVLQPAKLKDPAFLEELRALAPDLQVVVAFRMLPEVVWSLP
                     PMGTINLHGSLLPDYRGAAPINWAIINGETETGVTTFQLKQDIDTGNILMQESVSIGP
                     TETAGELHDRMKEIGARLLVRTIAGLAAGTLRERPQPNPAIPKHAPKIFTETCRIDWT
                     RPTSVVYDLIRGLSPSPAAFTEFNGKTLKIFRAEKDPDGAAAVPGLPDTDNRSYLRFA
                     TADGYIRVTELQLEGKKRMKTEEFLRGYQPRARGS"
     gene            complement(1245..2561)
                     /locus_tag="VKU83_07730"
     CDS             complement(1245..2561)
                     /locus_tag="VKU83_07730"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HLI93483.1"
                     /translation="MRAACLLVMILLLACGAGGQPPGSAAKPKPPNDGPSHILTGADQ
                     MDRYLPLLEGQSVAVFANATSVVGNRQLVDTLLKRGIRIKKIFSPEHGFRGDADAGAT
                     VGNGTDSATGIPVVSLYGRKLQPTAEDLAGVDVMLFDIQDVGVRFYTYISSMQKFLEA
                     AITNNRPLIILDRPDPNGFYVDGPVLDTKFRSFVGMQPVPVVYGMTIGEYARMLVGEQ
                     WLDPAVSSQLTTIHAINQSADRIDSLMRALHNRPVNVRLNDFRLIVIPCLHYTHKSRY
                     LLPVKPSPNLPNMQAVWLYPSLCLFEGTHISVGRGTAKPFQQFGSPYFPAGNYSFTPE
                     STPGATDPPLKGQVCNGFDLSRINVAKETGDRFSLKWILKAYSLFPGKTNFFSGNGST
                     FDRLAGTAILEEQIREGLTEEEIRKSWEPALSNFKKIRKKYLLYAD"
     gene            2668..3123
                     /locus_tag="VKU83_07735"
     CDS             2668..3123
                     /locus_tag="VKU83_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLI93484.1"
                     /translation="MAQEAQMGDTVKVHYLGRLTDGRTFDSSEGRPPLEFELGSGMVI
                     KGFDDGVRGMTVGQKKTIEIPAEFAYGPRDPEAVIEFPIDQFPPQMKLEIGMQLVMTD
                     NSGNQIPVSVAEVRPEAVVLDANHQLAGQDLIFDLELVEIKGASRIIIP"
BASE COUNT          681 a          874 c          996 g          739 t
ORIGIN      
        1 atgccgccga gcagcggacg agggagatcg gtgtccgcaa agtgttggga gccgggatcg
       61 ccaccatcgt ccggatgctg tcgatggact ttatcaaatt ggtgctgcta tccatcgtga
      121 tcgcttcgcc gctggcctgg ctggccatgc acaactggct ggagggtttc gcctaccgga
      181 tcggcatctc ggtatggata ttcattatcg ccggattgat cgctttgctc atcgccgtcg
      241 taaccgtaac ctttcaaacc gtcaaggcag cggtggccaa tcccgtggat agtttgaaga
      301 ccgaatgaac cggcgggtct tacgacccgc gggcgcgggg ctggtagccc cgcagaaatt
      361 cttccgtctt cattcgcttt tttccctcca gttgaagttc ggtcacccgg atataaccgt
      421 cagcggtggc gaaccgcagg tagcttcgat tatcggtgtc tggcaggccg ggaacggctg
      481 cggcgccgtc cgggtctttc tcggcgcgaa agatttttaa ggtcttcccg ttgaattccg
      541 taaaggcggc aggagatggg gaaagcccgc ggatgagatc atagacaacg cttgttggcc
      601 tcgtccagtc gatccggcat gtttccgtaa agattttcgg ggcatgtttc ggtattgcgg
      661 gattaggctg cggtctttcc cggagggtgc cggcagcgag gcctgcgata gtgcggacga
      721 gcaggcgggc gccgatctcc ttcatgcggt catggagctc accggcggtc tcggtcggtc
      781 caatggagac ggactcctgc atcaggatat tcccggtatc gatatcctgt ttgagttgga
      841 atgtcgtgac gcccgtctcc gtttcaccgt tgatgatggc ccagttgatg ggagcggccc
      901 cccggtagtc aggcaggagg gaaccgtgca ggttgattgt gcccattggc ggcaagctcc
      961 atacgacctc gggcaacatg cggaaggcaa cgaccacctg caggtcggga gcgagggcgc
     1021 ggagctcttc caggaacgcg ggatccttta gcttagcggg ctggaggaca cgcagatcat
     1081 gttcgcgggc aaattttttc acggcgctct ccataagctg catcccgcgg cctgccggct
     1141 tgtccggcgc ggtaatgacg cctgcgatgg gatagcccgc acggtggatc gcatccaggg
     1201 acgcgacggc gaagtcgggc gttcccatga atacaatgcg gggcctagtc tgcatacagc
     1261 aaatatttct tccggatctt tttgaaattg cttagtgcgg gttcccagct ttttcggatc
     1321 tcctcttccg ttagtccttc ccggatctgt tcttcgagga tggcggtgcc cgcaagccgg
     1381 tcaaaggtac ttccgttacc gctaaagaaa tttgttttcc cgggaaaaag ggaataggct
     1441 ttcaagatcc atttcagact aaacctgtcg ccggtttctt tggcgacgtt gatccggctt
     1501 aggtcaaagc cgttgcaaac ctgccctttc aatggcggat ccgtggcgcc gggcgtactc
     1561 tcaggggtga atgagtagtt accggcggga aagtacgggc tgccaaactg ctgaaacggc
     1621 tttgcggtcc cccgcccaac gctgatgtgt gtgccttcaa aaaggcaaag cgacgggtaa
     1681 agccagaccg cctgcatgtt gggaaggttt ggcgaaggct ttaccggcag caggtaccgg
     1741 cttttatgcg tatagtggag gcaggggatg acgataagtc tgaaatcgtt gagcctcacg
     1801 tttacaggac ggttatggag cgccctcatg agggaatcga tgcggtctgc ggattggttg
     1861 atcgcatgga tcgtggtcag ctggctgctg acggcaggat caagccactg ctctccgacc
     1921 agcatgcggg catattcccc aatggtcatc ccgtagacga cggggacggg ctgcatgccc
     1981 acaaacgatc ggaattttgt atcgagcacc ggaccgtcga catagaaccc gttcgggtcc
     2041 ggccggtcca ggatgatcag cggccggttg ttcgtaatcg ccgcctccaa aaatttctgc
     2101 atggaagaga tataagtata gaacctcacg ccgacatcct gaatgtcaaa taacataacg
     2161 tccacgcctg cgagatcttc cgcggtcggc tggagcttgc ggccataaag agacacgacg
     2221 gggatcccgg tcgcggagtc tgtcccgttg ccgacggtgg ctcccgcatc tgcgtcaccc
     2281 cgaaagccat gttcgggcga aaagatcttt ttgatccgga tccctctttt taacagggtg
     2341 tctaccagct ggcggttgcc taccacggag gtggcatttg caaatacggc taccgactgg
     2401 ccttcgagca ggggaagata tcggtccatc tgatcggctc cggtcaggat atgggatggc
     2461 ccatcattgg gtggcttagg ttttgcagcg gaaccagggg gctggcctcc ggctccgcag
     2521 gctaacaaga ggatcatcac caaaagacaa gcagctcgca tggttcattt ttgctttagg
     2581 ggtggtcgga acgcgccgct aatttaatta tttaatccta ccttcacgcc gtacccccac
     2641 attgggaata aacaaaaaaa tgaagacatg gcacaagaag cgcaaatggg agataccgta
     2701 aaggtccatt atctgggtag gttgacagac ggcagaacgt tcgattcgtc cgaagggcgg
     2761 ccgccgctgg agtttgagct tggaagcggc atggtgatca aaggttttga cgacggggtg
     2821 cgtgggatga ccgtcggtca aaaaaagacg atcgagattc ccgcagaatt tgcctatggg
     2881 ccgcgtgacc ccgaggcagt tatcgaattt cctattgacc aattcccgcc gcaaatgaaa
     2941 ctggagatcg gaatgcagct ggttatgaca gataattccg gaaaccagat ccccgtttcg
     3001 gtcgccgaag tcaggccgga ggcagtggtg ctagatgcca atcaccagct ggcgggccag
     3061 gatctcatct tcgatctcga gctcgtcgag atcaaaggtg cttcgcgcat cattatacct
     3121 tagtagattc cgcgccggcc gccgtacgac gtccggtggc gggagttgca gcgggtggcg
     3181 gcgcgtttcc attcttgcta tatttacgct cccaggccca tgccgtcaca agcagggcgg
     3241 tcagcagcaa tagcgtcatc aggatcgagc cttccaggcc gtagtttccc
//
LOCUS       DATGKE010000448         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_146295, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000448 DATGKE010000000
VERSION     DATGKE010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146295"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..825)
                     /locus_tag="VKU83_07740"
     CDS             complement(4..825)
                     /locus_tag="VKU83_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93485.1"
                     /translation="MNNILIATDFSPAANGAANYAAKLAAALHAKLTLVSAFQETPVP
                     VDGIVALGSTNFGELTQYSLEEEVDRLRLQQPLVVESLARPGATTPAILEAAEETHAD
                     LIVAGMSSTGKGTRKVFGSTATALARRTTLPLLIVPETAEFTPPAAIALADDVIREKD
                     RALPALLKDLLDRFHSRFFLIRIFNEGAGEIVEILHQNGPERMIGAFSPFADAPAGKP
                     LVHALDKFVGSYPIDILVMRPQPKSLVEQWLFGSHTRDMIFETNIPLLIIPEKIR"
     gene            1088..1735
                     /locus_tag="VKU83_07745"
     CDS             1088..1735
                     /locus_tag="VKU83_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93486.1"
                     /translation="MKNKLKMARAILLLLPFFLVMLPSCGPSVRLTASWSDPHVQPVR
                     FSKILVLAFGKDLEKRKLGEDYIKSELQRQGIAAGTSLDVFGPEFAGQYDSTKMRRAL
                     LDGQFDGAVTIRVLNIHERDRWVPGGVYYGPIGYYRGFYGYYHRVWGYYEQPDYTVTN
                     VEVLLESNLYRVATGELLWSGQSKAFSRNPTPEMAAEYAVNIVNDMSRKGVLAKP"
     gene            complement(2139..>2470)
                     /locus_tag="VKU83_07750"
     CDS             complement(2139..>2470)
                     /locus_tag="VKU83_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI93487.1"
                     /translation="LLSNAIKYSPERSAIRFMLEFEKDAVRFSVTDTGIGIPAEEQQH
                     LFQRFFRASNVLGVSGTGLGLSIVKKYLDLMGGTIHVESKPGAGSTFAIMLPAAPVLQ
                     DQVHALP"
BASE COUNT          551 a          568 c          763 g          588 t
ORIGIN      
        1 cctttagcgg atcttttcag gaatgataag tagcgggatg tttgtttcga agatcatgtc
       61 gcgggtatgg ctgccgaaca gccattgttc cacaagggat tttggctgag gccgcatgac
      121 caggatatcg atggggtagg aaccaacgaa tttatccagt gcatgaacga ggggtttacc
      181 ggcaggggca tcggcgaagg gactgaaagc tccgatcatc ctttccgggc cgttctggtg
      241 gaggatctca acgatctcgc ctgccccttc gttgaagatg cggatcagga agaacctgga
      301 atggaaccgg tccagcaggt ctttcaacaa agccggcaat gcccggtctt tttcccggat
      361 gacgtcgtct gcgagggcga tagctgccgg tggggtaaat tctgccgttt cggggacgat
      421 gagtaaggga agagtggtcc ttctggccag agcggtagcg gtgctgccga aaactttgcg
      481 tgtgcctttg ccggtgctgc tcatgccagc gacgatgagg tcggcgtgcg tttcttccgc
      541 ggcctccagg atggccggtg tggtggctcc cggtctggcg aggctttcga cgacgagtgg
      601 ctgctgtagc cgcagtcggt ccacctcctc ttccaggctg tattgggtaa gctcgccgaa
      661 attggtgctg cccagcgcca cgataccatc gacgggtacc ggcgtttcct gaaatgcgct
      721 gaccagcgtg agtttagcgt ggagggcggc ggcgagcttt gcggcgtaat tggcggcccc
      781 gttggcggcc ggagagaaat cggttgctat gaggatgttg ttcatacttt tttttcaaaa
      841 gtaaccctat tgtccggctt gtttgttgac gctcgtcagc agccggcctg atagtgggct
      901 gagggaagaa gatcatgtgg cggggtgacg gaggtaatga caatcagcgg agccgttgac
      961 atcgatcagg ggagcggagt aataccaatg gtagctttag ctaaactttt taattatgga
     1021 gaaaatactc ctgactattg acagccagaa cctgtggcga atttttaacg tataaacaat
     1081 tcgatttatg aagaacaaac ttaagatggc aagggccatt ctgctgctct tgcctttctt
     1141 cctggtaatg ctcccgtcgt gcggaccttc cgttcgcctg acggcatcct ggagcgatcc
     1201 tcacgtccag ccggtacgtt tttccaagat acttgtcctg gctttcggga aggatctcga
     1261 aaaacggaag ttgggtgaag attatatcaa aagcgaattg caaaggcaag gtattgcagc
     1321 ggggacaagc ctcgatgtgt tcgggcctga atttgccggt cagtatgact cgacaaaaat
     1381 gcggcgggcg ttgcttgacg ggcagttcga cggagcggta actatcaggg tacttaatat
     1441 ccatgagcga gaccgttggg tgccgggcgg tgtatattat ggcccgatag gatactaccg
     1501 cgggttttac gggtattatc accgggtttg ggggtattac gaacagccgg attatacggt
     1561 cacgaacgta gaggtactcc tggaaagcaa tctgtaccgg gtggcgacgg gggaacttct
     1621 atggtccggg caatcgaaag cattcagcag gaaccctacg ccagaaatgg cagccgaata
     1681 tgcagtgaac attgtgaatg atatgagccg gaaaggggtg ctcgccaaac cgtgacgaat
     1741 attagcggct gatatgaccg tcgtcattgt gggagatctt cgggtggaat aatttagtgg
     1801 tactataacg gcagagaagc atgtatgccg ctacggcgat agccgacatt gttttggctc
     1861 gagcccgtca cttttgaaac gtggcgggct cttttatcat ccgacggcgc tgttgaatat
     1921 gcattgaagc tggcttcggt gctcaacgat tttatccgga cgcaaaatat tcaaatgata
     1981 gcgatggttc ctcaccggca ttcgtttccg gaaagatggt tcatcaagag ccatacacgg
     2041 gatatgctat tcgaggcgag cgtacctttg ctgctgttgc cggacagcaa cagatgacgg
     2101 aagggatcgg agcgggccgg ggggaaggag cgttcgtgtc agggaagggc gtggacctgg
     2161 tcctgtaaaa ccggagcggc cggcagcatg atggcgaatg tcgaaccggc gccgggtttg
     2221 ctttcaacat ggatagtgcc gcccatcagg tcgaggtatt tttttacgat actgagccca
     2281 aggccggtac cggagacgcc caagacgtta ctcgcccgga aaaaacgttg gaaaaggtgt
     2341 tgctgttcct cggccggaat gccgatgccg gtatccgtta cggaaaaacg caccgcatct
     2401 ttttcgaatt cgagcatgaa ccggatcgca ctccgttccg gagagtattt aatggcgttg
     2461 ctcagaaggt
//
LOCUS       DATGKE010000449         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_234727, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000449 DATGKE010000000
VERSION     DATGKE010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234727"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VKU83_07755"
     CDS             <1..354
                     /locus_tag="VKU83_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLI93488.1"
                     /translation="LLGSLNPISVPAVNIAEQKDHYVVSLAAPGMKKDDFKIDIDGNT
                     LTISAETEETKEEKEDKYTRKEFNYSSFSRSFSLPDWVNKDKIDATYENGLLKLTLPK
                     TEEARKLSTRHVSVK"
     gene            complement(419..1468)
                     /locus_tag="VKU83_07760"
     CDS             complement(419..1468)
                     /locus_tag="VKU83_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93489.1"
                     /translation="MKKILLIDNDPLFRSNIAEILSLSHYEVVLAETAKAGIETALTN
                     RPDLIICDTALPAADGYTVLHSMQHDPHTSGIPFILLTGTTEKEDWRKAMAMGADDYL
                     TKPVDGVELLRAVETCLRKEENRKKTAIPAATTDETVTGENEAFLEELAQRTAHPYRK
                     KQVIYLEGQRPAFVYFVVSGKVKVYRSDPDGKDLITDIHSAGDFFGYPALLEGKNFSD
                     NAQALEDSGIVQIPRQEFTCWMSSRPEIARRFIHLLSREAAAREARFLNLAYNSLRKR
                     VANGLLQLASVDVQQRGHKEALAISRENLANIVGCATESLTRTLSDFRSEKLVDIRNG
                     RIYLLDESKLRNLIN"
     gene            complement(1446..2390)
                     /locus_tag="VKU83_07765"
     CDS             complement(1446..2390)
                     /locus_tag="VKU83_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HLI93490.1"
                     /translation="MPAIVTVTFHPCIDVNIEVPALLPDVKMLCSSVLRQPGGGGINV
                     ARAIHELGGKATAVYPAGGCTGTELSKLLAAEGVSTVILKIPGETRENIIVRDTATGL
                     QYRCNLPGPSVPELVLSELLELLGKWEAVKYLVVSGSLPPGMDAGVFQILADLARRKG
                     IRLIVDTSGEPLKAAIAAGAYLVKPSLHELLSLTTGRPDGSDVESRIDSLLSGGRTKA
                     AVVSLGPKGALLATGNCRQRFPAPKVTPVSTIGAGDSLVAGIVLALHRGMSLEQAVAY
                     GCLCGSAATLHAGTGLCRLEDVNRLKKTTERHEKDPVD"
     gene            2543..3016
                     /locus_tag="VKU83_07770"
     CDS             2543..3016
                     /locus_tag="VKU83_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLI93491.1"
                     /translation="MLGILNPPEIENVLKRQIIGRIGCANGDSTYVVPISYAYEGQFI
                     YCHTHEGMKIDCMRKHPNVCFEVDDLHDMANWESVIAWGTYEELSDPELRKIALLRLH
                     ERIMPFIASETVHLSPDWPFPPRELEKIPGITFRIKLTSKTGRFEMTDKQAFYAS"
     gene            3026..>3773
                     /locus_tag="VKU83_07775"
     CDS             3026..>3773
                     /locus_tag="VKU83_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93492.1"
                     /translation="MISFFTISIRALRFGTAGMRTMGMMVLFLWAGLDADAQSMRFIT
                     IDQAWRLAEKNYPLTARRQLLAKARDFSVSNAAKGWYPQLSISGQATLQSDVTNFPVK
                     LPVPGFSFPAYSKDQYRVYAEADQALYDGGVIRDQRLGALVSEQIDQKGLEVDLYALR
                     DRIDQLYFGILLLQQQVRQNALLQADILNGIEKTQALIDNGTAMRSALDELNAQLIQT
                     DQQRIQEETTGDAYLKMLGLLTGLALDRSTV"
BASE COUNT          850 a          978 c         1073 g          872 t
ORIGIN      
        1 ctgcttggat ccctgaatcc gatcagtgtt cccgcggtca atattgcaga gcaaaaagat
       61 cattacgtgg tcagtttggc tgctccgggg atgaaaaaag acgatttcaa gatcgacatt
      121 gacggaaaca ccttgacaat cagcgcagaa acagaggaaa ccaaagaaga aaaggaggac
      181 aaatataccc gtaaggaatt caactacagc tcgttctcaa ggagtttctc gttaccggat
      241 tgggtgaaca aggacaaaat tgatgcaacg tatgagaatg ggttgctcaa attgaccctg
      301 ccaaagacgg aagaagcgcg gaagctctcc acgaggcacg tcagtgttaa atgaccttgg
      361 ttgtattgga ggttcgtgcg atcccccgtc gttgcaagcg gcgggggttt ttgtttgctt
      421 agttaataag attccggagc ttgctttcat ccaggaggta aattctgcca ttccggatgt
      481 cgacaagttt ttctgatcta aagtcactca gggtacgcgt cagggattcg gtcgcgcaac
      541 ccacgatatt ggcgaggttc tcgcgcgaga tggccagggc ctccttgtgg ccgcgttgtt
      601 gaacatctac cgaagcaagc tgcagcagcc cattggccac gcgttttcgc aaggaattgt
      661 aggcgaggtt caggaacctt gcttcccggg cggcggcttc tcttgaaagc aggtggataa
      721 aacgccgggc gatttccggg cggctgctca tccagcaggt gaattcctgg cgggggatct
      781 gaacgatgcc tgaatcttcc agggcctgcg cattatccga aaaattcttt ccttccagaa
      841 gcgcgggata gccaaagaag tcgccggccg aatggatgtc tgttatgaga tctttcccgt
      901 ccggatcgct tcggtagacc ttgacttttc ccgatacgac aaagtacacg aaggcaggcc
      961 gttggccctc gaggtagatg acctgtttct ttctgtatgg gtgcgcggtg cgttgagcca
     1021 gttcttccag gaaggcttca ttttcgcccg tcaccgtttc gtcggtcgtc gcggcaggta
     1081 tggcggtttt ttttcgattt tcttccttac gcaagcaggt ttcgaccgcc cttagcagct
     1141 ccacgccatc cacgggcttg gtcaggtaat catcggcgcc cattgccatg gcctttcgcc
     1201 aatcttcctt ttccgtcgtg cccgtgagga ggataaaggg aatgccggaa gtatgcggat
     1261 catgttgcat ggaatgcaaa acggtatagc cgtcggcagc gggaagggcg gtatcgcaga
     1321 tgatgaggtc cggccggttt gtaagggcag tctcgatgcc tgcctttgcc gtttccgcca
     1381 gcacgacttc gtagtgggac aggctcagaa tttccgcgat attacttcta aatagtgggt
     1441 cattgtcaat caacaggatc tttttcatgt ctttcagttg ttttttttag tcggtttacg
     1501 tcttccagcc ggcatagacc ggtgccggcg tgcagcgtcg cagcactgcc gcagagacat
     1561 ccataggcta ctgcctgctc cagggacatg ccgcggtgca gggcgagtac gatcccggca
     1621 accaggctat cgccggcgcc aatggtactg acaggcgtaa ctttcggtgc gggaaagcgt
     1681 tgcctgcaat tgccggttgc gagcaaggcg ccctttgggc cgagcgacac gacagccgcc
     1741 tttgtgcggc caccggacag caggctgtcg atccttgatt ccacatcgga accatccggt
     1801 cgtcccgtcg tcagggatag cagctcatga agactgggtt tgacaaggta tgcgccggca
     1861 gcgatggcgg ccttcagagg ttccccggag gtatctacga tcaggcggat ccctttcctc
     1921 cgggcaaggt ctgccaagat ttgaaatacc cccgcgtcca tgccgggagg caagctgcca
     1981 ctaaccacca ggtattttac cgcttcccat ttccccagca gttcgaggag ttcgctgagg
     2041 acaagttccg ggacggacgg gccgggcagg ttgcaacgat attgcaagcc ggtcgctgta
     2101 tcgcggacga tgatattctc cctggtttcg cccgggattt tcaggatgac cgtactcaca
     2161 ccttcagccg cgagaagttt gcttagttcg gttccggtgc agcccccggc cggatagacg
     2221 gctgtggcct tcccgcccaa ttcatggatg gcccgggcca cattaatgcc gccacctccg
     2281 ggctgccgca gtacggacga gcagagcatc tttacatccg gcagcagggc agggacctcg
     2341 atattcacgt cgatgcaggg gtggaaggtc accgtgacga tagcgggcat aaattttttc
     2401 agcaaactag cgcaggcggt ccggcacgct tatgattcgg taccccgaag aaatatgaat
     2461 tttatcacgt ccgggattga tgatggtcag ggaccggccc gtaaccggct tctacctttg
     2521 gaatggtaaa aatcttacga ctatgctcgg aatactcaat ccgccggaaa tcgaaaatgt
     2581 gctgaaaagg cagattattg gccggatcgg ttgcgccaac ggtgattcaa cgtacgttgt
     2641 gcccatcagc tatgcctatg agggccagtt catttattgc catacgcatg aaggaatgaa
     2701 gattgactgt atgcgaaagc acccgaacgt ttgcttcgaa gtggatgatt tacatgatat
     2761 ggccaattgg gaaagcgtga tcgcgtgggg gacatacgaa gaattgtctg atccggaatt
     2821 aaggaaaatc gcactcctca ggttgcatga aaggatcatg ccttttatag ccagcgagac
     2881 cgtgcatctg tccccggact ggccgtttcc tccgcgcgag ctggagaaga ttccggggat
     2941 cacctttcgt atcaaattga cgagcaagac cggcagattc gagatgacgg acaagcaagc
     3001 gttttacgcc tcctgatatg gggttatgat cagcttcttc accatatcaa tccgggccct
     3061 tcgttttggg acggcgggta tgaggaccat gggtatgatg gttctgttcc tgtgggccgg
     3121 tttggacgcc gacgcccaaa gcatgcgctt cataaccatc gaccaggcct ggcggctggc
     3181 agaaaagaat tacccgctga cggccaggcg gcagctgctg gcgaaagcca gggatttttc
     3241 cgtatcgaat gccgccaaag gctggtaccc gcaactcagc atcagcggcc aggccaccct
     3301 tcagtcagac gtaaccaatt tccccgttaa attgcctgtc ccgggcttca gctttccggc
     3361 gtacagcaaa gatcagtatc gtgtttatgc tgaagcagac caggcgctgt atgatggcgg
     3421 ggtcatccgg gaccagcgcc tgggagctct tgtcagcgag cagatcgatc aaaagggatt
     3481 ggaggtcgac ttgtatgccc ttcgcgatcg cattgatcaa ctatatttcg ggattctctt
     3541 attgcaacaa caggtccggc aaaacgcgct attgcaagcc gacattttga acggcataga
     3601 aaagacccag gcgcttatcg ataacggaac tgcgatgcgc agcgccttgg acgaactcaa
     3661 tgcgcagctt attcagaccg accagcaaag gatccaggaa gagacaaccg gggatgctta
     3721 tttgaagatg ctgggattgc tgaccggcct agcactcgac agaagcacgg tgc
//
LOCUS       DATGKE010000450         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1247547, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000450 DATGKE010000000
VERSION     DATGKE010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247547"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="dprA"
                     /locus_tag="VKU83_07780"
     CDS             complement(<1..945)
                     /gene="dprA"
                     /locus_tag="VKU83_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLI93493.1"
                     /translation="MEDRSVYQLALRSIRGIGDVHTRTLIDHFGDAESVFRKDRKDLE
                     KTGLRPDLIDAISHFRDFASLRPQVSRLQQRGIRILFYHQPDFPRRLAKLSTAPALLY
                     YQGNADLNAGKVVAVVGTRKPSPYGVQMARQITLELARPEMLIVSGLAFGIDAIAHQA
                     ALDSGIPTVGVLGTGLERIYPPEHTGLAQRMQKNGGLLSSFRCDAEGATFSFPLRNRL
                     VAGLCDALIVVESGIPGGSLSAAETAAELNKNCFAVPGRLTDEKSRGCLKLIHEGTAR
                     PLLSATQLCGVMGWNYPAGGSAHQPELPLPASGRQDPLS"
     gene            complement(1065..2978)
                     /locus_tag="VKU83_07785"
     CDS             complement(1065..2978)
                     /locus_tag="VKU83_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI93494.1"
                     /translation="MKNRVLFSILLLFITAKATWAQYDADKINKKAVSLYEKALDLAE
                     DNVTGAIALLQQAVRIDSNYEEAYLSIAGMYGSLKDYDKAVTYYEKARAIDSNFFKDY
                     NLPYSIDLAGAGRFDKALEAVNIFLTDPRLSETSKKAGEYRQRCYQFALSYAATHPVS
                     GYRFEPRNLGDSINTDVSEYFPTITIDGNTLIFTRRVHHINEDFYESHKTGGVWSKAV
                     SLPGNINTNNNEGAQNVSQDGQWLLFTGCDFPDGRGSCDLYISYLTADGWSTPENLGD
                     SINTEFWESSPSLSPDKKDLYFASGQPDGYGGSDLYVSHRHLNGKWSLPENLGPTVNT
                     IGNEAAPFIHADNQTLYFTSDGHPGYGNDDLFVTRKGPDGRWSKPENLGYPINTVDNE
                     GSLVVAADGKTAYYASDRADSRGGLDLYTFELREDIRPARTLWVKGRVFDSVTRKGLP
                     SSVILTDLATRQVISDLQTDETGNYLITLPEGKDYAFEVKRRGYLFFSENFALTGATG
                     DTAYHIDIPLQPLAANAVVVLKNIFFQSNKYDLQPESGTELDAVVQLMKDNPTLRIQI
                     SGHTDNSGNAADNLKLSANRARAVTAYLTGKGIASDRLVSKGWGDTKPIAENSTPEGR
                     ARNRRTELKVISR"
     gene            complement(3023..>3667)
                     /locus_tag="VKU83_07790"
     CDS             complement(3023..>3667)
                     /locus_tag="VKU83_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HLI93495.1"
                     /translation="PDQAPAAGSTGDQQSGPRLPVMERFYTLQGEGFHSGKAAYFIRL
                     AGCDVGCVWCDVKASWEVSRHPRVAVAELVREAAAFPGRLAVITGGEPLMHDCSHLTR
                     QLHEAGFATHIETSGAWPLTGNWDWICLSPKKFKAPLPGITALADELKVVIYNQSDFG
                     WAEKWAAETNPACHLYLQPEWSRSAAVTPLIIEYIKAHPAWRFSLQLHKYIHVP"
BASE COUNT          718 a          993 c         1164 g          792 t
ORIGIN      
        1 agagagcgga tcctgccggc cggaagcggg aagcggcagc tccggttggt gggctgaccc
       61 gccggcagga tagttccagc ccatgacacc gcagagctgg gtggccgaca ggagggggcg
      121 ggcggtgcct tcgtggatga gcttcaggca accgcggctt ttttcatcgg tgagccggcc
      181 ggggacggcg aagcagtttt tgttcagttc cgccgctgtt tcagcggcgg acaatgagcc
      241 gccgggtatt ccggattcga caacgatcag cgcgtcgcag agcccggcga cgagccggtt
      301 gcgaagtgga aaactgaagg ttgcgccctc ggcgtcgcag cgaaaggaac tgagcaggcc
      361 gccgttcttc tgcattcgtt gggccaggcc cgtgtgctcc ggcgggtaaa tgcgctccag
      421 gccggtgccg agaacgccga ccgtcgggat gccggaatcc aatgctgcct ggtgcgctat
      481 cgcgtcgatg ccgaaggcaa ggccgctcac gatgagcatt tccggcctgg ccagctccag
      541 agtgatctgc ctggccatct ggacgccgta aggactgggt ttccgggtgc cgacgaccgc
      601 gacgaccttg ccggcattga ggtccgcgtt gccctgatag taaagaaggg cgggcgctgt
      661 gctgagcttc gccagccgcc ggggaaagtc aggctgatga taaaagagga tccggatgcc
      721 cctttgctgc agccggctga cctggggccg gaggctggcg aaatcgcgga aatggctgat
      781 ggcatcaatg aggtccgggc gcagccctgt cttctccagg tccttccggt ctttgcgaaa
      841 gacggactcg gcatcgccga aatggtcgat gagcgtcctc gtatgtacgt cgccgatacc
      901 acggatactt cgcagtgcaa gctgatatac ggacctgtct tccacaccgg ttgaattgtc
      961 cgacaaggta agggaaagac aacggatgac aggccgaaac tggcttcgct gcagtatcca
     1021 tgcagtgggc aaaaccattc atggaaagcc gccggcgcgc gcgactagcg gctgatgacc
     1081 tttagctccg tgcgccggtt acgggcgcgc ccctcgggag tactgttctc cgcgatcggt
     1141 ttggtgtcgc cccagccttt tgagacgagc cggtcggaag cgataccctt gccggtcagg
     1201 taggccgtga cagccctggc gcggttggcg gacagtttca ggttgtcggc ggcgttaccg
     1261 gagttatcgg tatggccgct gatttgaatc cgcagcgtcg gattatcctt catcagctgg
     1321 acgacggcat cgagctcggt acccgattcg ggttgcaggt catacttatt ggattggaaa
     1381 aagatattct tgagcacgac gacggcattc gccgcaagcg gttgaagggg gatatcgata
     1441 tggtacgcgg tgtcaccggt cgcgccggta agcgcaaaat tttcggaaaa gaaaagatag
     1501 ccccgtcttt tgacctcaaa cgcgtagtct tttccctctg gaagggtaat cagatagttg
     1561 cccgtctcgt cggtttgaag gtcgctgatg acctgccggg tggcgagatc cgtgaggatg
     1621 acgctggagg gcaggccttt gcgggtaacg gaatcgaaga ccctgccttt tacccagagg
     1681 gtgcgtgccg gacgaatatc ctcgcgcaat tcgaacgtgt acaggtccag cccgccccga
     1741 ctgtccgcgc ggtcgcttgc atagtaggcg gttttgccgt cggcagccac gacgaggctg
     1801 ccttcattgt cgacggtatt gatcgggtag ccgaggtttt ccggtttgct ccacctgccg
     1861 tcggggcctt tgcgggtaac gaaaaggtcg tcattgccgt aaccgggatg gccgtcggac
     1921 gtgaaataaa gcgtttggtt atcggcgtgg ataaaaggcg cggcctcatt tccaatggta
     1981 tttaccgttg ggccaagatt ttccggcagg ctccatttgc cgttgagatg ccggtggctt
     2041 acatacaagt cgcttccacc atagccatcc ggctggccgc tggcaaaata caggtctttt
     2101 ttgtccggcg aaagcgaggg cgacgattcc cagaattcgg tattgatgct atcgccgaga
     2161 ttttcgggcg tgctccagcc gtctgcagtg agataggaaa tataaaggtc gcagctcccg
     2221 cgaccgtccg gaaaatcaca gccggtgaag agaagccatt gtccgtcctg ggagacattt
     2281 tgcgcgccct cattgttgtt ggtattgata ttcccgggaa ggctgacggc tttgctccag
     2341 acgccgccgg ttttatgcga ctcatagaag tcctcgttga tgtgatggac ccgccgcgta
     2401 aatatcaggg tgtttccatc aatggtaatg gtcgggaaat attccgaaac atcggtattg
     2461 atgctatccc ccaggttccg gggctcgaac ctgtagccgc tgaccgggtg cgtggcggcg
     2521 tagctgagcg cgaactggta acaacgctgg cggtattcgc cggctttttt gctggtctcg
     2581 cttagccgcg ggtcagtcag aaaaatattc acggcctcga gagccttgtc gaaacggccc
     2641 gcgcccgcca gatcgataga ataaggcagg ttgtagtctt tgaaaaaatt actgtcgatc
     2701 gcgcgcgcct tttcataata ggtgacggct ttgtcgtaat ctttcaggga accgtacatt
     2761 cccgcgatcg atagatatgc ctcctcatag ttgctgtcga tccggacggc ttgctggagc
     2821 agggcgatcg cgccggtcac attgtcttca gccagatcga gcgccttttc gtagagactt
     2881 accgccttct tgttgatctt gtcggcgtcg tattgtgccc aagtggcttt ggcggtgatg
     2941 aataaaagaa gaattgaaaa gaggacacgg tttttcatag gagtaaggta cagattttcg
     3001 gtgagcccgg caatcgcagt cgtcagggca catgaatgta tttgtgcagc tgcagggaaa
     3061 aacgccatgc cggatgcgct ttaatatatt cgatgatcag gggcgtaacg gcagccgacc
     3121 tcgaccattc cggttggagg tagaggtgac aggcggggtt ggtttcggcc gcccatttct
     3181 ccgcccagcc aaaatccgac tgattataaa tgaccacctt cagttcgtcc gcgagggcag
     3241 tgatacccgg gaggggcgcc ttgaattttt tgggggacag gcagatccag tcccagtttc
     3301 ctgtcagcgg ccatgccccg gaggtttcga tatgcgtggc gaagccggcc tcgtgaagct
     3361 gccgcgtaag gtgggagcaa tcgtgcatga ggggttcgcc gcccgtaatg accgccagcc
     3421 ggccggggaa agcggccgcc tcgcgtacga gttcggcaac cgcgacccgc ggatgccgcg
     3481 aaacttccca actggctttt acatcgcacc acacgcagcc gacgtcacag cccgccagcc
     3541 ggatgaagta ggcggccttg ccactgtgga agccttcacc ctgtagggta tagaagcgct
     3601 ccatgaccgg caggcgggga ccgctctgct ggtcgccggt actgccggcg gccggcgcct
     3661 gatctgg
//
LOCUS       DATGKE010000451         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1078164, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000451 DATGKE010000000
VERSION     DATGKE010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078164"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VKU83_07795"
     CDS             complement(<1..1099)
                     /locus_tag="VKU83_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLI93496.1"
                     /translation="MDQAQRLRELFDRYLHQSLLPAEIGELVLLLERADAQDILSEPM
                     RQVWEELRNRQVDHKVDWDKMLREMSRALPRGTQTSSENMARRRATLREVWGWRVVAA
                     FFFILAGIAAYWSVRWQGPQERSRAAAKRAGAGVKKTGPRQYFVSRSGSDEQDGSRRY
                     PWKSLAAVSRTALGPGDSVFLHGGQVFAGTLKIGRGPGDGGTPANPVWIGTYGGGEAT
                     VDGGDSSALVVYQAHSVVVTGLRLAGAGRKAGNVRDGLAIIESQDIRVGDVEITGFQK
                     SGLFIYASTRVVAERVYAHENGAAGIAVEGPSDRKTGSRDLKILYCRADDNPGDPTNL
                     TNHSGNGIVCGHCTKLVIDHCSATNNGWDMPR"
     gene            complement(1134..2072)
                     /locus_tag="VKU83_07800"
     CDS             complement(1134..2072)
                     /locus_tag="VKU83_07800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLI93497.1"
                     /translation="MKAIILKEFGGIERLQIAELANPGVGEEEVLVRVKAIGVNPVDA
                     KTRAGKGLAGMLKDDQPVILGWDISGTVEKSTSHLFTEGDEVFGTVRFPGHGKAYAEY
                     VVAPAEQLALKPENITHEQAAASCLAPLTAWQAFSDYGRLRPGQRVLIHAAAGGVGHF
                     AVQIARHIGAFVIATSSASNKDFVLSLGANEHIDYKTERFEDVAAEMDFVLDTVGPEN
                     ADRSLQVLKKGGTLICIAGLSDATKEKAKARGIFALGMTVQSAGEDMQHLAQLLEEGE
                     LKPEVSKVFGFGEMGEAHQQIESGRTKGKIVVRTDL"
     gene            2282..>2520
                     /locus_tag="VKU83_07805"
     CDS             2282..>2520
                     /locus_tag="VKU83_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93498.1"
                     /translation="MRKPTFSFINVFGLTIGLTSCILIGLFINDELGFDRFHANTGRI
                     ARVTGESSDNGTVSSLAVTGTRVGPRFKSIFPSVEA"
BASE COUNT          487 a          868 c          596 g          569 t
ORIGIN      
        1 tccgcggcat gtcccagccg ttgttggtcg cgctgcaatg gtcgatgacc agctttgtac
       61 agtggccgca aacgatccca ttcccgctat gattggtgag attggtagga tccccggggt
      121 tgtcgtccgc gcggcaataa aggatcttca gatccctcga gcccgtcttc cggtcactgg
      181 ggccctcgac agctatgccg gcggccccgt tttcgtgtgc atagactcgc tccgcgacga
      241 cccgcgtcga agcatatatg aaaagtccgg atttttgaaa ccccgtgatt tcgacgtcgc
      301 ccacccggat atcctgcgat tcgatgattg ccaggccgtc cctgacattt cctgccttcc
      361 ttcccgctcc ggccagccgc aggcccgtca ctacaaccga atgcgcctga taaacgacca
      421 gggccgaaga gtcgccgcca tcgaccgtcg cctcgccgcc cccataggtg ccaatccaca
      481 ctgggtttgc cggcgtcccg ccatcgcctg gcccgcgccc gatcttcaaa gtacccgcaa
      541 atacctgtcc gccgtgcaaa aacaccgaat ctcccggccc taatgccgtg cggctgaccg
      601 ccgccaggct tttccaggga taccgcctgc tgccgtcctg ttcgtcgctc ccactccggc
      661 tgacaaaata ctgccgcggt ccggtctttt tcacccccgc accggcacgc ttagccgcag
      721 cccgcgacct ctcctgcgga ccttgccacc tgaccgacca ataagccgca atgcccgcga
      781 ggatgaaaaa aaaggccgcg acgacccgcc atccccagac ctcccgcaat gtagcccttc
      841 gccgcgccat gttttccgaa ctcgtctgcg tccctcgcgg cagcgcccgg ctcatttccc
      901 tgagcatctt gtcccagtct accttgtggt ctacctgccg gtttctcagc tcttcccaaa
      961 cctggcgcat cggttcggat aagatatcct gcgcatccgc ccgctcgagc agcagcacca
     1021 gctcgccgat ctccgccgga agcaggcttt gatgcagata gcgatcgaat aattccctga
     1081 gtcgctgagc ttggtccata gctggtggtc acgctaaaag tagggaattc ctatcacaga
     1141 tccgtccgca cgacgatctt gccctttgtc ctgccgctct cgatctgctg gtgtgcctcg
     1201 cccatttcgc cgaaaccaaa gaccttcgag acttcgggct tcaattcacc ctcttccagc
     1261 aattgcgcca gatgctgcat atcctcgcct gccgactgaa ccgtcatgcc aagcgcgaag
     1321 atgccccttg ccttcgcctt ttctttggtg gcatcgctaa ggcccgcaat acagatcagc
     1381 gtcccgcctt ttttgagtac ctgaagggac cggtcggcat tttccggtcc caccgtatcc
     1441 aacacaaaat ccatttccgc ggccacgtct tcaaaccttt ccgtcttgta gtcgatatgt
     1501 tcattcgccc cgagcgacag gacaaaatcc ttgtttgacg cagaggatgt cgctatgaca
     1561 aaggcgccga tatgccgcgc aatctgtacg gcaaaatgcc ctaccccgcc ggccgccgca
     1621 tggatgagca cccgctgccc cggcctcagc cttccatagt ccgagaaggc ctgccaggcc
     1681 gtcaaaggcg ccaggcacga tgccgccgcc tgttcatggg ttatgttttc aggcttgagc
     1741 gccagctgct ccgctggcgc gaccacgtac tccgcatatg ccttaccgtg cccggggaac
     1801 cgcaccgtcc caaaaacctc gtccccttcc gtaaacaaat gtgacgtcga cttctccacc
     1861 gtcccggaaa tgtcccaacc caggatgacc ggctgatcat ctttcaacat ccctgccagg
     1921 ccctttcccg ccctcgtttt ggcgtccacc ggattgaccc cgatcgcttt gaccctgaca
     1981 aggacctctt cttccccgac cccaggattg gccaattccg cgatctgcaa ccgctcaatg
     2041 cccccgaact ccttcaaaat gattgctttc atccaataat ggtttatttg tccggcaaaa
     2101 ctaagggttt ctgtcaagca atactttaaa tccgcctgtt ttcccgccgc tgatttcctg
     2161 cggtggcctt gctgcgactg actggctgca accggcttac caccgcaagc aacatccgcg
     2221 cggaaattgc gtatattaaa tgatgctccg caattacctc aaacttgccc tccgccattt
     2281 gatgaggaaa cccacattct cattcatcaa tgtttttggt ctgacaatag gcctcaccag
     2341 ttgcatcctt atcggcctct tcatcaacga tgagttgggc tttgaccgtt tccacgcgaa
     2401 caccggccgc attgcgcgcg tgacaggcga aagttcagat aatggcaccg tttccagtct
     2461 ggccgttacg gggacccgcg taggaccgag gtttaagtcg atatttcctt cagttgaagc
//
LOCUS       DATGKE010000452         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_153622, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000452 DATGKE010000000
VERSION     DATGKE010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153622"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VKU83_07810"
     CDS             complement(<1..1547)
                     /locus_tag="VKU83_07810"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HLI93499.1"
                     /translation="MDADVIIVGSGLSGLVAGCELVSRGLRVLIIEQEPVQCLGGQAF
                     WSLGGLFLVGTPLQKRLGIRDSYELALNDWMATAGFDRMEDEWPRKWAEAYIQFAAGE
                     KYSWLRGLGIKFFPIVGWAERATGTASGRGNSVPRFHIAWGTGPAVLAPFVKQLEIAA
                     TGGRAQFRFRHQVDELVLSGGVVTGVRGTILEPSFIQQGERSSRAVTGDFSFSAQAVI
                     VASGGIGGNLELVRRNWPAHLGAPPVHLLRGVPEHVDGRMLGIVQDSGGNLINPDRMW
                     HYTEGVTNWAPVWKDHGIRILPGPSAMWFDAGGTRLPGPLYPGFDTTGTINYLRRTGH
                     DHSWFILNQKIIRKEFGLSGSEQNPELTGRDWRSMLRRAWSNGTAAPVEAFKQQGIDF
                     IVRKDLRELVAAANLLTQSPGLDYDLVREQIMARDLAARNKICTDGQILAIRGARRYL
                     GDKLIRTAPVHAILDPNAGPLIAVRLHLLTRKTLGGLETNLDAQVIDTTGRPIPGLYA
                     VGEAAGFG"
     gene            complement(2011..2478)
                     /locus_tag="VKU83_07815"
     CDS             complement(2011..2478)
                     /locus_tag="VKU83_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93500.1"
                     /translation="MTSVTAQQQELNVLKTKIAELQTALFYTESASPIKLPTHVVTDV
                     EIDGDGRIWFTVPRPAMHIDAYEKEVHAKLDFFKKGKDFFVKVKGSATLLIDAETINR
                     FDSLSAGLKDKMTDEKSIGIMVKIEDADFVDNTPRSSQNWLQASRSQLSSWFF"
     gene            complement(2642..3007)
                     /locus_tag="VKU83_07820"
     CDS             complement(2642..3007)
                     /locus_tag="VKU83_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HLI93501.1"
                     /translation="MAWVYLFFGAFFEMAWTLSLRYMDLKKVKTVHWKGFFQLHGDWR
                     IIWPLVGYVVFGLSNVYCISIAMKEIPASTALAVWMGTALVGVKLVEILFLKGTYDIY
                     QFLYIGLILLGIAGLKKNP"
BASE COUNT          741 a          879 c          767 g          756 t
ORIGIN      
        1 ccaaaccccg ctgcttcccc aactgcatac aatccgggaa tcggccgtcc ggtcgtatcg
       61 atgacttgtg catcgagatt ggtttccagc ccgcctaacg tcttgcgcgt aagaagatgc
      121 aatcttacgg caatgagcgg acctgcattc gggtcgagta ttgcatgcac cggcgctgtc
      181 ctgatgagtt tgtcacccaa atagcgccgg gccccgcgaa ttgctaaaat ttgaccgtcc
      241 gtgcaaatct tatttcttgc agccaggtcc cgggccatga tttgttccct cacaaggtca
      301 taatcgagtc ccggggactg ggtcagcagg ttcgccgcag cgacgagctc ccgcaagtct
      361 ttccgaacaa taaagtcgat accctgttgc ttaaaagcct caacaggcgc tgccgtgcca
      421 ttactccagg cccgcctcaa catcgatcgc cagtccctgc cggtcagctc gggattttgt
      481 tccgaacccg ataatccaaa ctcctttcga atgatctttt gattcagaat aaaccaggaa
      541 tgatcatgac cggtcctccg caggtagttg atcgtcccgg tcgtatcaaa cccgggataa
      601 agcggccccg gaagcctcgt gcccccagcg tcaaaccaca tcgcggaagg tcccggcagg
      661 atgcgaatgc catgatcttt ccagacaggc gcccagtttg tgactccttc cgtataatgc
      721 cacatcctgt ccggattgat aaggtttcct ccggaatcct gtacaatacc gagcattctg
      781 ccgtcgacgt gctccggcac gccccgcaag aggtggaccg ggggcgcccc gaggtgggcc
      841 ggccagttcc ttctgacaag ctcgaggttg ccgccgatgc ctccggaagc gacaattacc
      901 gcctgtgcac tgaatgaaaa atcgccggtc accgcccgcg agcttcgctc gccctgctgg
      961 ataaatgacg gctcgaggat ggtacccctc acgcccgtga cgaccccgcc cgagaggaca
     1021 agctcatcga cctggtgccg aaatctgaat tgagcccttc ctcccgttgc cgcgatttcc
     1081 aattgcttta caaacggcgc caggaccgcg ggtcctgtac cccaggcaat atgaaagcgt
     1141 ggtactgaat ttccgcgacc ggaagcggtc cccgtagcgc gttcggccca cccaacgatc
     1201 gggaagaact taatgccgag gccgcgcagc caggaatact tttctccggc tgcaaactga
     1261 atgtacgctt cagcccattt ccgcggccat tcatcttcca ttctgtcaaa tcccgcagtc
     1321 gccatccaat cgttcaacgc gagctcgtaa ctatctctga tacccagccg tttttggagc
     1381 ggcgtaccga ccagaaaaag accccccagg gaccagaacg cctggccgcc gagacattgt
     1441 acgggttcct gttcgatgat cagaacccgt aagccccgcg agaccagctc gcagccggct
     1501 accagtccgg agagaccact gccgacgatg atgacatctg catccatggg caaccaacct
     1561 ttccctaaag ataagcatcc cgcaaaagcg gccggtgagc tggaatgaaa accggcagat
     1621 ccgatggctg gaagcgcgtt gggcttcata tgcgccacat gctaaaaacg gctgtcggta
     1681 aaggttttat ggtttttttc ttcgttttga gatgatagaa ttaggcctaa actgtctgag
     1741 ggtccacagg aatgcgcact atatttgtag aagcaaaacc aaatttatcc aaaactcaat
     1801 atatagggtg cggaattgaa gattttcata tggcaagcaa tcgttagagg tcgactgttt
     1861 ctacagtgga ccttttttct ttaggcccat tccgctgggt tttaatgctt caggtatggc
     1921 tgaaaagccg aaatccgcga acaagtcgcg gactcagctt cctatatgta acacgcgcag
     1981 taaactcttc ggttgggcgc aggctgtact ttagaagaac cagctggaca actgcgacct
     2041 gctcgcctgg agccagtttt gtgaagacct gggagtattg tccacgaaat ccgcgtcttc
     2101 gatcttcacc ataatgccta ttgatttctc atcagtcatt ttgtctttca gtccggccga
     2161 caatgagtcg aagcggttga tggtttcagc atcgattagc agggtggccg atcccttgac
     2221 ctttacgaaa aagtccttcc cttttttaaa gaaatccagc tttgcatgca cttccttttc
     2281 atatgcatcg atatgcatgg cgggcctggg caccgtaaac catatccgac cgtcaccgtc
     2341 aatttctacg tcggttacga cgtgagtcgg cagcttgatc ggcgaagccg attccgtata
     2401 gaataatgcc gtttgcagtt cggcgatctt ggttttcaat acgtttagtt cttgctgctg
     2461 agcggttacg gatgtcatgg ctttagtgtt tgggtacaga agattttttg atttccggga
     2521 acggtggatg taagtcaatt ggcataccag ttcacaaccc attgaacttc aataaaactc
     2581 cagatggttt acccttagat gaatcccagt ttgggacggc ttttccaaat tgcgcggccg
     2641 gctacgggtt tttttttaat ccggctattc cgagaaggat caaccctata tacaggaatt
     2701 gatagatatc gtaggttcct ttgaggaaca ggatctcaac cagttttacc ccaaccagcg
     2761 cggtccccat ccaaacagcc agggcggtag aagcgggaat ctctttcatg gcgatggaga
     2821 tacaataaac attactcaac ccgaatacga cgtatccgac aagcggccaa atgatccgcc
     2881 aatccccgtg aagttggaag aaacctttcc agtgcaccgt ttttaccttt ttcaggtcca
     2941 tgtaccgcaa tgaaagggtc caggccatct caaagaaggc cccgaaaaac aagtacaccc
     3001 atgccatgct gcccgctttt gcagattgtg aataatttat ttatccagat acggtaaaag
     3061 gacctgctgc cagatcctgt aacccttctc attcatgtgc agtttgtcgc cgaggaagag
     3121 actgtctatc ggctgccctg tcg
//
LOCUS       DATGKE010000453         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_729692, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000453 DATGKE010000000
VERSION     DATGKE010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729692"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VKU83_07825"
     CDS             complement(<1..448)
                     /locus_tag="VKU83_07825"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HLI93502.1"
                     /translation="MDRPKVLVVAGYDQSAGAGVLSDIKTLEAHRVYGYGVCTGITFQ
                     NETAVRRVDWLRLEDITAQIDLCYESSGFSWVKMGITPSAKVAAAIIDHLRLRNPAIK
                     VILDPVIRSSSGADFWGATPQLEWEALAARCYLVTPNWEEIEWLYPA"
     gene            complement(441..1121)
                     /locus_tag="VKU83_07830"
     CDS             complement(441..1121)
                     /locus_tag="VKU83_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93503.1"
                     /translation="MDMPDFLLAVITAPGQISGEAGSLEAMLEAGVQRVHLRKPGGSV
                     QALAERLAPRWASRLVLHGSEDLARDYGIGQVHGPVRVPAGGQGGEAAGAGQAEAASG
                     EPGPGELGGAGGGTRFPGRVRIRRSTSVHSWEDLRGLPEGLEYAFISPLFDSISKPGY
                     GATAGLLDMPGGPYPCRPVGLGGVSAGNIELMRSLGWKGAALMGWLWESPGETVRRFK
                     QIKKIVDG"
     gene            complement(1121..2986)
                     /gene="thiC"
                     /locus_tag="VKU83_07835"
     CDS             complement(1121..2986)
                     /gene="thiC"
                     /locus_tag="VKU83_07835"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HLI93504.1"
                     /translation="MKKDVIPGRQVISREPFPASRKIYVPGKLHAIRVAMREIRVGDT
                     VDSYAQTVTPNPAITVYDTSGPYTDPAVPIDVREGLPRLREDWIMGRGDVEQMEQVSS
                     LYGRRRQEDTALDGLRFSHLRPPKRAKAGLAVTQLAYARRGIITPEMEYIAIRENQRI
                     EEYAGLGKLNHQHPGHSFGALTPKHLVTPEFVRDEVAAGRAVIPSNINHPESEPMVIG
                     RNFLVKINANIGNSAVSSSIEEEVEKAVWACRWGADTIMDLSTGKNIHETREWILRNA
                     PVPVGTVPIYQALEKVGGKAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTA
                     SRMTGIVSRGGSIMAKWCLAHHKESFLYTHFGEICEIMRAYDVSFSLGDGLRPGSIAD
                     ANDAAQFAELETLGELTKLAWKQDCQVIIEGPGHIPMHLIKENMDKQLRECQEAPFYT
                     LGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKKDVKDGVITYKI
                     AAHAADLAKGHPGAQYRDNALSKARFEFRWQDQFNLSLDPDTAKSFHDETLPAEGAKI
                     AHFCSMCGPHFCSMKITQEVRRFAAEKGLDEQEALGEGMREKAEAFKEAGSEIYL"
     gene            complement(3062..3268)
                     /gene="thiS"
                     /locus_tag="VKU83_07840"
     CDS             complement(3062..3268)
                     /gene="thiS"
                     /locus_tag="VKU83_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HLI93505.1"
                     /translation="MIEVSINQRNYSVPDTGTLADVLPLLDIRQPDGVAIALNEVVIP
                     KGEWGVHVLRPQDRVFVIRATQGG"
     regulatory      complement(3323..3418)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3477..>4328)
                     /locus_tag="VKU83_07845"
     CDS             complement(3477..>4328)
                     /locus_tag="VKU83_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HLI93506.1"
                     /translation="SGPNADDYHWTEVLMQNIPLYRTWGLSMHYYTIAGDWEHKGSAT
                     QFGEDKYFKAAEACLKMDEIINKHSAIMDKYDPMKRVALAVDEWGIWTDVEPGTNPAF
                     LHQQNSLRDALIAGTTLNIFNNHADRVRLAALAQTVNVLQSLVLTDKDKMLLTPTYYV
                     FDLYKVHQDARSLVVQLGSPDYTYGGRRIPAVNVSASKDSAGVIHISLVNLDPVNSFR
                     VDASLPGLTFSGVTGQILSSGKFTDVNEFDQPAKVRPVAFDGARKQGDGLVVTLPAKS
                     IVLLELK"
BASE COUNT          840 a         1443 c         1102 g          943 t
ORIGIN      
        1 cggcggggta aagccattcg atctcttccc aattgggcgt caccaggtag cacctggcag
       61 ccagggcctc ccattccagc tgaggtgtcg ccccccaaaa gtcggcgccg ctgctcgacc
      121 tgatcaccgg atccaggata acctttatcg ccgggttgcg cagccgaaga tgatcgatga
      181 tcgctgccgc cactttcgcc gaaggcgtga ttcccatctt aacccaggag aatcctgacg
      241 actcgtagca cagatcgatc tgcgcggtga tgtcctccag gcgcagccag tccacccgcc
      301 ggaccgcagt ttcgttttga aacgtgatcc cggtacagac tccgtaccca tacacgcgat
      361 gcgcctccag cgttttgata tccgacagga cgcccgcccc cgcactctga tcgtagcccg
      421 cgacgaccag cacctttggc ctatccatca acgatctttt ttatttgttt gaagcgccgc
      481 accgtctcgc ccggcgactc ccacagccaa cccattaatg ccgcgccctt ccatcccagc
      541 gaccgcatca gttcgatatt gccggcgctc acgccgccca gcccaaccgg ccggcaaggg
      601 taaggccccc ccggcatgtc cagcagtccc gctgtcgcgc cgtaccccgg ctttgaaatg
      661 ctgtcaaaga gcgggctgat aaaggcatat tcaagcccct ccggcaatcc cctcaaatct
      721 tcccacgaat gcacggacgt ggatcgcctg atccggaccc gcccgggaaa tcgcgtcccg
      781 ccaccggcac ctcccagctc acctggaccc ggctcgccgg atgcggcctc cgcttgccct
      841 gcacctgccg cttccccgcc ctgcccacct gccggcaccc tgaccggccc gtgtacctgg
      901 ccaataccat agtctcgcgc caggtcctca ctcccgtgca gcaccaaccg cgatgcccag
      961 cgcggggcaa gccgttccgc cagcgcctgg accgaaccgc cgggctttct cagatgcacg
     1021 cgctgcacgc ccgcctccag catcgcttcc aacgaccccg cctcgccgga aatttgtccg
     1081 ggtgcggtga tcactgccaa caggaagtcc ggcatatcca tcacagataa atttcggagc
     1141 cggcctcttt gaacgcctcc gccttctcgc gcatcccttc gcccaatgct tcctgctcgt
     1201 cgaggccctt ttccgccgca aaccggcgga cctcctgcgt gatcttcatc gaacagaagt
     1261 gcggaccaca catgctgcag aagtgcgcga tcttcgcacc ctcggccggt aaggtctcat
     1321 cgtgaaaaga cttcgcggta tccggatcca gggaaaggtt gaactggtct tgccagcgga
     1381 actcgaacct cgccttgctc aacgcgttat cacggtattg tgcgcccgga tgccccttcg
     1441 ccaggtccgc cgcatgggcc gcgatcttat aggtgatcac cccgtccttt acgtcttttt
     1501 tgttcggcag tcccaaatgt tccttcggcg tcacatagca gagcatagct gtgccaaacc
     1561 atccgatcat cgcggccccg atcgccgacg tgatgtgatc gtatcccggc gcaatatccg
     1621 tggtcagggg accaagggtg taaaacggcg cctcctgaca ctccctgagc tgcttgtcca
     1681 tattctcttt gatgaggtgc atcggaatgt ggcccgggcc ctcgatgatg acctggcagt
     1741 cctgcttcca tgccagtttc gtcaactcgc ccagggtctc cagttccgca aactgtgcgg
     1801 cgtcattggc atcggcgata gatcccggtc tcaacccgtc gcccagcgag aaggaaacat
     1861 cataggcgcg catgatctcg cagatctcgc caaaatgcgt atagaggaaa ctttccttat
     1921 gatgcgccag gcaccatttt gccataatgg acccgcccct ggagacgatg cccgtcatcc
     1981 gcgatgccgt aagcggaatg tatcgcagca ggaccccggc atggatcgtg aaataatcga
     2041 ccccctgctc cgcctgttcg atcagcgtat ccctgtagat ctcccaagtc aggtcctcgg
     2101 ccttgccgcc gaccttttcc agcgcctggt aaataggtac ggtgcccacc ggaacgggtg
     2161 cattgcggag tatccattcc cgcgtttcat gaatattttt tccggtggat aggtccatga
     2221 tcgtatcggc gccccaccga caggcccaga ccgccttttc cacctcttct tcgatgctcg
     2281 agctcacggc ggaattgccg atgttggcgt tgatcttgac gaggaaattg cgtccgatga
     2341 ccatgggctc ggactccggg tgattgatat tgctgggaat gaccgccctg ccggccgcca
     2401 cttcgtcgcg aacaaattcc ggcgtcacga ggtgctttgg cgtcaatgcg ccaaaactat
     2461 gtcccggatg ctgatgattc aacttgccca ggcccgcata ttcctcgatc cgttgatttt
     2521 ccctgatggc aatgtattcc atctccggtg taatgatgcc gcgccgcgca tacgcgagct
     2581 gtgttaccgc cagccccgcc tttgcccttt tcggcggccg gagatgcgaa aaccgcaacc
     2641 catcgagcgc cgtatcctcc tggcgccgcc ggccgtatag ggaactgacc tgctccattt
     2701 gttccacatc gccgcggccc atgatccagt cttcccgcaa ccggggcagg ccctcccgca
     2761 cgtctatcgg gacggcgggg tcggtgtatg gcccgctggt atcatagacg gttattgccg
     2821 gattgggcgt caccgtttgt gcatagctgt cgactgtgtc gcccacccgt atttcgcgca
     2881 tggcgacccg gatagcatgc aactttcccg gcacataaat cttcctcgac gcggggaatg
     2941 gctcccggct gatgacttgc cgcccgggaa taacgtcttt cttcatataa ttaaagtttt
     3001 agatcataca ttgagccttc gtgcgccgga actccactgc gccggaactc cactgcgccg
     3061 cttagccgcc ctgcgttgcc ctgatcacga atacccggtc ctgcggccgc aatacatgca
     3121 ccccccattc ccctttcggg atcaccactt catttaaggc aatcgctact ccatccggct
     3181 gccggatatc cagcagggga agtacatccg ccaatgttcc cgtgtcagga accgaataat
     3241 ttctttgatt gatcgatacc tctatcatct cgcttcgatt attccgcaat caacgataag
     3301 aggcgccgcg aacccggcaa aatgagaaag tttacctttt cccttcgccg gcattacccg
     3361 gatcaggttc tgagggtatg ttctcagcct gcggcggcag gcacccctaa agtcctaagc
     3421 caaagttaag ctgtcggcaa aaaattgtca aatattaatt tttccctgcc gcggatctat
     3481 ttgagctcca gcaagacgat cgatttcgcc ggcagcgtca ccaccaggcc gtctccctgc
     3541 ttcctggcgc cgtcgaatgc caccggcctg acctttgccg gctggtcaaa ctcattgacg
     3601 tcggtaaatt ttcccgaact gaggatttga ccggtcacgc ccgaaaaagt cagtcccggc
     3661 agggacgcat ccaccctaaa gctattcacc ggatccaggt tgacgagcga aatatggatc
     3721 actccggccg agtccttcga tgcggacacg ttcacggccg ggatgcggcg gccaccatag
     3781 gtatagtccg ggctcccgag ctgcacgacc agcgaccggg catcctggtg gaccttgtac
     3841 agatcaaaaa cataatacgt cggtgtaagc agcatcttgt ccttatccgt caataccagc
     3901 gactgcagga cattcaccgt ctgcgcgagc gccgcaagcc tcaccctgtc cgcgtggttg
     3961 ttgaaaatat tcagtgtggt acctgcaatc agcgcatccc ggaggctgtt ttgctgatgc
     4021 aggaacgcgg ggttcgtccc gggctcgacg tctgtccaga tgccccattc atccaccgcg
     4081 agcgcgaccc gcttcatcgg gtcgtattta tccattatcg cggaatgctt gttaatgatc
     4141 tcatccatct tcaggcaggc ctccgcggcc ttgaagtact tatcctcacc aaactgcgtg
     4201 gcagacccct tgtgttccca gtcccccgcg atcgtataat agtgcatgga caggccccat
     4261 gttcggtaca aagggatatt ctgcatgagc acctccgtcc agtggtaatc gtccgcattg
     4321 gggccgct
//
LOCUS       DATGKE010000454         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_606464, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000454 DATGKE010000000
VERSION     DATGKE010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606464"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VKU83_07850"
     CDS             complement(<1..725)
                     /locus_tag="VKU83_07850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI93507.1"
                     /translation="MKKIRILEGIRQGRIGGGESYLLGLVENLNRDHFEPVVLSFTDG
                     PMVDRLRAQGIDTRVIHTEQPFDIRIWKKVKNLIADLDVDIVHAHGTRACSNLFWAAR
                     RLRVPMIYTCHAWSFHLDQNPLVKKMRIWGEEFLTSRTDVNICGCEANKNTGKQFFRD
                     FDAVVINNSIDSKRFNPYEDYKDVRAELGIGKDELVIVSVARFTSQKQPLKLIRAFEK
                     VAGKVPDVRLLMIGDGEEKQSAV"
     gene            complement(760..1926)
                     /locus_tag="VKU83_07855"
     CDS             complement(760..1926)
                     /locus_tag="VKU83_07855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLI93508.1"
                     /translation="MDLLFLPQTVEPNQRVSPRYAFRSYYVPQSTATLQYSGFYYIGK
                     NSVHIDKLVQIYKRGYASEGIENTKAELLRCLDREKNVLPEVIFCEANFDISEIRGLK
                     QFLRQHPVLNSIPFILDGSGLGDRELELYRTYARPDEILFLQHCDQAVLNDKIAFLRK
                     MKKGQHGAAPQIEENVPARSLRLGLLSKRSFDILVSSIAILLLSPLFLLIAIAIKLES
                     SGPVFYIAKRAGRGYRIFNFIKFRTMYQGSDKRMGELTHLNQYTAVTQGPVFFKISND
                     PRITKVGSFLRNTSLDELPQLFNVLLGDMSLVGNRPLPLYEAATLTTDEYAARFMAPA
                     GITGLWQIKKRGNKMMSVEERISMDIDYARKCNFATDFWIIANTPSALLQKENV"
     gene            complement(1980..2381)
                     /locus_tag="VKU83_07860"
     CDS             complement(1980..2381)
                     /locus_tag="VKU83_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93509.1"
                     /translation="MKKQILAVDDSKAIRFLLQTVLGRDYSVITVPDGYSAMYYLSHR
                     AQPDLIIADPQLPDMDNWELIQQLSTSGMYGDIPMMVLSGLPEQETEAKCSEYGVVRY
                     FLKPFNPVEMIEAIDRMVTSGELNRDISYAS"
BASE COUNT          577 a          663 c          552 g          597 t
ORIGIN      
        1 ttaaccgccg actgtttctc ctccccgtcg ccgatcatca gcaaccggac atccgggact
       61 tttccggcca ctttttcaaa tgcccgtatc agcttcaacg gttgcttctg cgaagtgaac
      121 cgcgcaaccg aaacgataac cagctcgtcc tttccgattc ccagctccgc cctcacgtcc
      181 ttatagtctt cataaggatt gaagcgcttt gaatcaatgg agttattgat cacgaccgca
      241 tcaaagtccc ggaaaaactg cttgccggta ttcttattgg cttcgcagcc gcaaatgttc
      301 acgtcggtcc tggatgtcaa aaactcctcc ccccagatcc gcatcttttt taccagcgga
      361 ttctgatcga ggtgaaaaga ccatgcgtgg caagtataaa tcattggtac ccgcagcctg
      421 cgggctgccc aaaacaggtt cgaacaagcc cttgtcccgt gcgcatgcac aatgtcgaca
      481 tccaggtctg cgatcagatt cttaaccttc ttccaaatgc ggatatcgaa cggctgctct
      541 gtatgtataa cacgcgtatc aatgccttgt gcccgaagac gatccaccat cggcccgtcg
      601 gtgaacgaca gcaccaccgg ctcgaaatga tcccggttca gattctccac cagccccaaa
      661 aggtaactct ccccacctcc gatcctgccc tgccgtatac cctccaggat acgaatcttt
      721 ttcatggcac aatcaattgt tggtacaatg attcggtatc tatacgtttt ccttctgcag
      781 taacgctgaa ggggtgtttg caattatcca aaagtccgtt gcaaagttac actttctggc
      841 ataatcgatg tccatgctga tccgctcttc gaccgacatc atcttgtttc cccgcttctt
      901 gatctgccag agtcccgtaa tgcccgcggg cgccatgaac cgcgcggcat actcatccgt
      961 cgtcagtgtt gccgcttcgt acaacggcag gggacggttt cccaccagcg acatatcgcc
     1021 cagcaacaca ttgaacagtt gcggcaattc gtcgagactg gtattccgga ggaaactgcc
     1081 gaccttcgtg atccgcggat cgttgctgat cttgaaaaag accgggccct gtgtgacggc
     1141 agtgtattga ttgaggtgcg tcaattcgcc catccgcttg tcagagccct gatacatggt
     1201 cctgaatttt atgaaattaa agatccggta accccggccg gcgcgcttgg cgatatagaa
     1261 gaccggcccg ctcgactcca gcttgatcgc gatcgcgatc agcaggaaca agggactcag
     1321 taacaatatg gcaatggacg agaccaggat gtcaaacgac cgcttggaca gcagccccag
     1381 cctgaggctc cgggcaggga cattttcttc gatctgcggt gctgcaccat gctgcccttt
     1441 tttcatttta cgcagaaaag cgatcttgtc attcaggacg gcctgatcac aatgttgcag
     1501 gaaaaggatc tcatcgggcc gggcataggt ccgataaagc tcgagctccc ggtcgcccag
     1561 tcccgatccg tccaggatga aagggatgga attcagtaca ggatgttgcc ggaggaactg
     1621 cttcaggccg cggatctcgg aaatgtcgaa attcgcctcg caaaaaatga cttccggaag
     1681 gacgttcttt tcgcggtcga gacacctgag gagctccgct ttggtgtttt cgatcccttc
     1741 cgaagcataa cctctcttgt aaatctggac cagcttgtca atgtgaacgc tgttttttcc
     1801 aatatagtag aaaccactat attgaagtgt cgccgtagac tggggcacat aataggaacg
     1861 aaaggcgtag cgtggagaaa ccctttggtt cggctctaca gtttggggta aaaataataa
     1921 atccatacgt caggtttacg gtttcatata tacggtaatc aaatcgttaa tatcattttt
     1981 tagctggcat aggaaatatc cctgttcagc tcgccgctgg ttaccatcct gtcgatggct
     2041 tcgatcattt ccaccggatt gaacggcttg aggaagtatc taaccacccc gtattcagag
     2101 cacttcgcct cggtctcctg ttcgggcagg ccagatagca ccatcatcgg aatatcgcca
     2161 tacatgccac tggttgacaa ttgctgtatc aattcccaat tgtccatgtc cggcagctgg
     2221 ggatctgcaa tgatcagatc gggctgcgcc ctatgcgata aataatacat tgctgaataa
     2281 ccatccggaa ccgtaatgac cgaataatcc cttcccaaaa cagtctgcag cagaaaacgg
     2341 atcgctttac tatcatcaac agccagaatt tgctttttca tttaccaat
//
LOCUS       DATGKE010000455         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_812509, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000455 DATGKE010000000
VERSION     DATGKE010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812509"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VKU83_07865"
     CDS             complement(<1..1232)
                     /locus_tag="VKU83_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93510.1"
                     /translation="MLKSFFFTAALAAGVSLASAQTLTMPDYIQKAYSNGTRDMDGKP
                     GRNYWQNHGTYTISLTVAPPDRTIRGVEEIVYTNNSPDTLHGLNMKLIQNVHRAAGRR
                     GTPGDTAEGIRVDEFSIRGQQVRWDNEAATTTNYMVNLDQPLMPHDSLKLNITWHYEL
                     SKQPGREGVIDSTTFYIAYSYPRVSVYDDYMGWDTQPHTGGFEFYNDFNNYELNVTVP
                     GNFVVWSTGTLQNPTEVLQPEAAARLQQSFTDDSTVHVATAQQMAAKQITTQNATNTW
                     KWTANDISDMALGISDHYDWDAASVVIDDKTGRRVSMQAAFADASADFHHSVQFGRYS
                     LRWFSNNWPGVDYPYPKSTAFQGFADMEYPMMMNDSHEPDLAFAELVQDHEQAHTYFP
                     FYMGTNESRYGFMDEGWAT"
     gene            complement(1386..1814)
                     /locus_tag="VKU83_07870"
     CDS             complement(1386..1814)
                     /locus_tag="VKU83_07870"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI93511.1"
                     /translation="MKSINPWINFNGNAETAFAFYKSVFGGEFKKIVRFKDLAGPEFP
                     VAEKEANKVMYIALPIGKNNVLVANDVPEAMGRTNENENRSKILVSVESREEADKIFN
                     GLSAGGSVEGPIGDTPWGSYAGMFRDKYGIEWIVEFDPQS"
     gene            complement(1945..2796)
                     /locus_tag="VKU83_07875"
     CDS             complement(1945..2796)
                     /locus_tag="VKU83_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLI93512.1"
                     /translation="MIKEWMQEYQPKNQDEAQAALREIMQEVALAGLQRAGFFEKAAF
                     YGDTALRIFHGLDRFSEDLDFSLLEVNPGFSLEPYFKGMMNEFEAIGMDVSIKTRNKK
                     SGSNVESAFLKSETIWKELILEGVIVPSGLESLPKIKIKLEVDREPPAGFSTEEKLLL
                     KPFSFYVKCYRLPDLFAGKMHALLFRSWQLRVKGRDWYDLEWYIKKGVPLHIGHFLSR
                     ARDSGNWKMSHISKAQILELLRNKIEAVSFDRIREDIIRFIPNASALDIWSPRYFKDL
                     ADKIKFE"
     gene            complement(2793..3431)
                     /locus_tag="VKU83_07880"
     CDS             complement(2793..3431)
                     /locus_tag="VKU83_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93513.1"
                     /translation="MNFEQAIQEYAEEPLTRQVIKDMLKDYQRPNDKISELIKAGELT
                     ALKNGLYIAGPKTKTPMPEPFLVANHLRGPSYVSMESALAYWGMIPERTFEVISVTTK
                     AARAYQTPIGRFSYIHAALPYYAYGIRSVALTPRQVALIATPEKALCDKIVQTSGILL
                     RSPGQTLDFLLEDLRIDEEVLTRLDVKEMDSWSAHTPKHSSIEMLIKTLRNL"
     gene            3649..>3951
                     /locus_tag="VKU83_07885"
     CDS             3649..>3951
                     /locus_tag="VKU83_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93514.1"
                     /translation="MFQKLYQHLEQINANTRRQQADLEHAEVWAAKISQQLDLLNASI
                     LALKTQPPRRRAPATGFVLGGLAVLLLGMLAIYAYQLSAVADAVRDRSAATVRAYGV"
BASE COUNT          866 a         1053 c          986 g         1046 t
ORIGIN      
        1 gttgcccagc cctcgtccat aaacccgtac cggctttcgt tcgtgcccat gtagaagggg
       61 aagtaggtat gtgcctgctc gtggtcctgc accagctccg caaacgcaag gtccggctcg
      121 tggctgtcgt tcatcatcat cgggtattcc atatcggcga agccctggaa ggccgtggat
      181 ttcgggtagg gatagtctac gcccggccag ttgttggaga accaccgcaa cgagtacctg
      241 ccaaactgca cggaatgatg aaagtccgcc gaggcgtcgg caaaggccgc ctgcatgctc
      301 acccggcgcc ccgttttatc gtcgatcacc acgcttgccg catcccagtc atagtggtcg
      361 ctgatcccca gcgccatgtc gctgatgtcg ttggccgtcc atttccaggt gttggtggcg
      421 ttttgcgtgg tgatttgctt agccgccatc tgctgtgcgg tagcgacatg gaccgtggaa
      481 tcgtcggtga aggactgctg cagtctcgcc gcggcctccg gttgcagtac ttccgtcggg
      541 ttttgcagcg tacccgtcga ccagacgacg aaattccccg ggacggtgac attcagctca
      601 tagttattga aatcgttata gaactcgaag ccccccgtat gcggctgcgt gtcccatccc
      661 atataatcgt cgtacaccga gacgcggggg tacgagtagg cgatatagaa cgtggtggaa
      721 tcgatcaccc cctcccggcc cggctgcttc gataactcgt agtgccaggt gatgttcaac
      781 ttcagcgagt catgcggcat cagcggctgg tcgaggttca ccatatagtt ggtggtcgtg
      841 gccgcctcat tgtcccaccg cacctgctgc ccgcggatcg agaactcgtc gacccggatg
      901 ccctcggcgg tgtcgcccgg cgtgccgcgg cgtccagcgg cccgatggac gttttggatc
      961 agtttcatgt tcaggccatg gagggtgtcg gggctgttat tcgtatacac gatctcttcg
     1021 acgccgcgga tcgtcctgtc cggcggcgcg acggtcaggg agatggtata ggtgccatgg
     1081 ttctgccagt aattcctgcc cggcttgccg tccatgtcgc gggtgccgtt ggaataggct
     1141 ttctggatgt agtcgggcat ggttagggtc tgagctgagg cgagtgaaac gccggcggcg
     1201 agggcggcag tgaaaaagaa tgacttaagc atagggcaaa tataggcggt tttcggagcg
     1261 ggatagtgga ttgtgatatg cggccgtttc cagtgaggaa tgacggtgga ggggatagtg
     1321 ggtcgcgaaa agcctcagat gaatatccga ggcttccaat tatatccgac gaataaattc
     1381 attctttatg actgggggtc aaattccact atccattcaa tcccgtattt gtctctgaac
     1441 attccggcgt aggaacccca gggcgtgtcg ccgatgggtc cttcaacact gccgcccgcc
     1501 gacaggccat taaatatttt gtctgcttct tcccggcttt ccacgctaac caatatttta
     1561 gacctgttct cgttttcgtt cgtacgcccc atggcttcgg gaacgtcatt ggccaccaac
     1621 acgttgttct tgccgatggg caaagcaatg tacattacct tatttgcctc cttttcggca
     1681 accggaaatt ccgggcctgc gaggtctttg aaacgaacga tctttttgaa ctctccgcca
     1741 aaaaccgatt tgtagaaagc gaatgctgtt tcggcgttgc cattgaagtt gatccaggga
     1801 ttgattgatt tcatgatgtt tatttttgtt gtttttttat tgttgccaaa taagcaaccg
     1861 ataggttgca aatatattcg caacttttca gttgcgcaaa tttattttga tttttttggg
     1921 tgaattttac aaatggtgtg cgttctattc aaattttatc ttatctgcca aatctttgaa
     1981 ataccgtggc gaccagatgt cgagtgcgct ggcattgggg ataaaccgga taatatcctc
     2041 acggatccgg tcaaatgaca ccgcttctat cttgtttcgc agcagttcca atatttgggc
     2101 cttggagata tggctcattt tccaattgcc tgaatcccgc gcacgtgata aaaagtggcc
     2161 gatatgcaat ggaactccct ttttgatata ccattccaag tcataccaat cccttccttt
     2221 tacccgcagc tgccagctcc gaaacagcaa agcatgcatt tttcccgcaa acagatctgg
     2281 tagcctataa catttgacat aaaaagaaaa gggtttcaac aaaagctttt cttcggtact
     2341 aaaacctgcg ggcggctccc ggtccacctc cagtttaatc ttgatttttg gcagtgattc
     2401 caacccggac gggacaatga cgccttcgag aatcagctcc ttccaaatgg tttccgactt
     2461 caaaaaggcc gactcgacat tagatccgga cttcttgttt cttgttttaa tgcttacatc
     2521 cataccgatc gcttcaaatt cattcatcat tcccttaaag tatggctcga gggaaaaccc
     2581 tggatttact tccagcaacg aaaaatccag atcttctgaa aatctgtcga ggccgtggaa
     2641 aatccgcagc gccgtatcgc cgtaaaaggc tgccttttca aaaaagccgg cccgttgcaa
     2701 gcccgccaac gccacttcct gcatgatctc tctcaacgct gcctgcgcct catcctggtt
     2761 tttcggctga tattcctgca tccattcctt gatcataggt tgcgcagggt tttaatcaac
     2821 atctcgatac tactgtgttt tggcgtatgg gctgaccagg aatccatttc ctttacatcc
     2881 agccgggtta gtacttcttc gtcgatcctc aaatcctcga gtaaaaaatc aagcgtctga
     2941 ccggggctcc tgagcaaaat tccagacgtc tgaacaatct tgtcacaaag cgccttttca
     3001 ggagtagcga ttagcgccac ttgtctgggc gtgagcgcaa cactccttat tccatatgcg
     3061 tagtaaggta aagcggcgtg aatatagctg aacctgccaa taggcgtttg ataagccctg
     3121 gccgctttcg tcgtaaccga aataacttca aaggttcgct cagggatcat tccccaatat
     3181 gccaaagcag actccatgga cacgtaactc ggccccctca gatgattcgc caccaaaaag
     3241 ggttccggca tgggcgtttt ggtcttgggc cccgctatat acaagccatt cttcaaggct
     3301 gtcagttcgc ccgccttgat cagctcactt attttgtcgt ttggccgctg ataatccttg
     3361 agcatatcct tgataacctg ccgcgtcaac ggttcctctg catactcctg tatggcttgc
     3421 tcgaagttca tgacaaattt taacgcaaaa atcggagttt ttccgataaa tcgacatatt
     3481 atttacatgt tatgcaaatt tttacataca taatcggaat tattccgatt atcctataat
     3541 tttttatcat ggtggaaagg atatttccgc atttcgccag gttccccggc cctccaaggc
     3601 cttcgcaggg aacgggtttt gcagttttga ccgcatcaat tgaagccaat gttccaaaaa
     3661 ctctatcagc atctcgagca gataaatgcc aatacgcgcc ggcaacaggc cgatttggag
     3721 cacgcggagg tctgggccgc aaagataagc cagcaactgg acctcctgaa tgcctcgatc
     3781 ctggcgctga aaacacaacc gccgcgccgc cgtgctcctg ctacgggctt tgtccttggc
     3841 ggactggccg tcctgttgct gggcatgctg gcgatctacg cttaccaatt gtcggcggta
     3901 gccgatgccg tgcgtgaccg gtccgcagct accgtccggg cctacggcgt g
//
LOCUS       DATGKE010000456         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1193603, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000456 DATGKE010000000
VERSION     DATGKE010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193603"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1952
                     /locus_tag="VKU83_07890"
     CDS             141..1952
                     /locus_tag="VKU83_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI93515.1"
                     /translation="MKKTLLYILAGMTVVAGSSCTKKILEKVNVNGLPGQEVWSDSTL
                     STLYLNALYNVVMPYWPANESSTTFPGSFHNTSDESNGGTTAILQGKLTSESVADFYP
                     STSAGAYPYVRRINMLLANIDNYGLKPSQTVITKAQAYFLRAFIYFDLLKIYGGIPYI
                     TKPLDWETDSLNLPRNSTSQCVDSIVADLNQCSVLPATWSGADFGRITRAAALAFKAR
                     VLLTWASPQFNPTNDMSRWQAAYTAAQHAYDTCTLDGYGLYPNFSRIALDVASGTDRE
                     PILFRPFNGTNVSGQYEGYDGVTRPYSQSAGSGGQTNNPTWNLVQAFPMKDGYPITAS
                     SSTYKYDSVYYWHNRDPRFYATIVYNGAVYGLGSVSGRKQWLYTNISVDKGHVTNTGF
                     YCRKNIDTTVSAVNSQYGKTFWVEMRFAEVVLTLAECANMTGNQAQAYTLLESIRKRA
                     GITANADGYYGLQQNMTQAQLETAILNEREVEFAFEGKRYDDLRRTRTFDQLNGSYRQ
                     QIVIAFKSPYTVATMEKINAVTGVAPVDTINVDGPDYTKYFTHSFQAISGELPINFLT
                     TYYAYAIPSSNISKEPAMQQTIGWLFAGGAGTFDPTK"
     gene            1999..>2216
                     /locus_tag="VKU83_07895"
     CDS             1999..>2216
                     /locus_tag="VKU83_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93516.1"
                     /translation="MKKRFLLIAAIAGCVGITRPALAQYPTVPEDVKAQADAMLKEAY
                     RRSDSAWQVAWPIIDNDAKHGKPYIPWAA"
BASE COUNT          527 a          581 c          620 g          488 t
ORIGIN      
        1 gacgggtatg aatctctgga gtattattag tcccacgcct tatcgggatc cccgttcgaa
       61 tgagatcacg gattacccga tccttcgcac ctggacattt ggtttgaacg tgaacttata
      121 gtatttaaaa aagtacgaca atgaaaaaga cgcttctata tatactcgca gggatgacgg
      181 ttgttgccgg cagttcctgt accaaaaaga tactcgaaaa agtaaacgta aacggtcttc
      241 ccggtcagga ggtctggtcc gactcgacgc tgtctacgct gtatctgaac gccctgtaca
      301 atgtagtgat gccgtattgg cccgccaatg aaagcagcac cactttcccc ggttcgttcc
      361 acaataccag cgacgaaagc aatgggggga ctacggcgat acttcagggg aaactgacga
      421 gcgaatcggt cgccgatttc tatccttcta catcggccgg cgcctatccc tatgtgcgcc
      481 ggatcaatat gctcctggcc aacatcgaca attacggact gaaaccgtcc cagacggtga
      541 tcacgaaggc ccaggcttat tttctacggg cgtttatcta cttcgatctg ctgaagatct
      601 atggagggat cccctatatc accaaacccc tggattggga gaccgatagc ctgaatttgc
      661 cgcgtaacag cacgtcgcag tgtgtcgaca gcatcgtagc cgatctcaac caatgctccg
      721 tgctgcctgc gacctggtcg ggagcggatt tcggccggat cacgcgggct gcggcactgg
      781 ctttcaaggc gagggtcctg ctaacctggg ccagccctca gttcaatccg accaacgata
      841 tgagccggtg gcaggcagcc tatacggccg cgcagcatgc gtacgatacc tgtacgctgg
      901 atggttacgg tttgtatccc aatttttccc ggatcgcgct ggatgttgct tccggcaccg
      961 atcgcgagcc tatccttttc aggcccttca acgggaccaa tgtcagcgga caatatgaag
     1021 gctatgatgg cgtgacccgg ccgtattcgc agtcggcagg cagcggcggg cagaccaata
     1081 acccgacgtg gaacctggtt caggccttcc cgatgaaaga cggttatccc atcacggctt
     1141 cctcttctac ctataagtac gattccgttt attactggca taaccgagat ccgcggttct
     1201 atgcgacgat cgtatacaat ggggccgtct acggtctggg cagcgtcagc ggccgcaagc
     1261 agtggctgta cacgaacatc agcgtggaca agggccatgt taccaatacc ggtttctatt
     1321 gccggaagaa tatcgatact acggtatccg cggtcaactc gcaatatggc aagacgttct
     1381 gggtagagat gcgtttcgcg gaggtagtgc tgacgttggc ggaatgcgcc aacatgacgg
     1441 gcaatcaggc gcaggcgtat acgctgctgg aatcgatccg caaacgggcg ggaataaccg
     1501 cgaatgcgga tggatattat ggtttgcagc aaaatatgac ccaggcgcag ctggagacgg
     1561 ccattctcaa cgagcgggaa gtggaattcg ccttcgaagg gaagcggtat gatgacctgc
     1621 ggcggacacg gacgttcgat cagctcaacg gaagttaccg gcagcagatc gtgatcgctt
     1681 tcaagtcgcc gtataccgtg gccactatgg aaaagatcaa cgctgtgacg ggtgtggctc
     1741 cggtagatac catcaatgtc gatggtccgg attacaccaa atattttacg cattcgttcc
     1801 aggcgatcag cggcgagctc ccgatcaatt tcctgacgac gtattatgcc tatgcgatac
     1861 cgagcagcaa tatcagcaag gagccggcca tgcagcagac gatcggctgg ctgtttgcgg
     1921 gaggcgcggg tacgtttgat ccgacgaagt agggggcggc cggagggacc tgagcgagat
     1981 tatttaaatt acatataaat gaagaaacga tttttattga ttgcggctat agcaggttgt
     2041 gtcggtatca cccggccggc ccttgctcag tacccgactg ttcccgagga tgtcaaggca
     2101 caggcagatg ccatgctcaa ggaagcctat cgccggtccg attcggcatg gcaggtggca
     2161 tggccgatca tcgacaacga tgcaaaacat ggaaaaccgt atataccctg ggcggc
//
LOCUS       DATGKE010000457         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_770851, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000457 DATGKE010000000
VERSION     DATGKE010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770851"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VKU83_07900"
     CDS             complement(<1..502)
                     /locus_tag="VKU83_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HLI93517.1"
                     /translation="MHKERQQDRQEEAPAADGSEELYERLCLKVDRGQDPLRIDKFLV
                     QRIENASRNKIQKALEAGMVLVNNKQVPSNYKIRPLDEIVLYSDKETHGEEIIPEPLP
                     LNIRYEDEEVMIINKPAGIVVHPAIGNYTGTLINGVAWYLRQKNAALSEDTLPRFGLV
                     HRIDKNT"
     gene            539..1249
                     /gene="lipB"
                     /locus_tag="VKU83_07905"
     CDS             539..1249
                     /gene="lipB"
                     /locus_tag="VKU83_07905"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLI93518.1"
                     /translation="METVVFQDLGRMEYQAAWDYQEALLQESLRLKASGRDTGHYLLF
                     VEHPPVFTLGKSGHPENVLIGEERMREKGVQFFRTNRGGDITFHGPGQLVGYPILDLE
                     KFYTDIGRYLRDLEEVTIRVLAAYGICGDRSKGETGVWIEPGVKGRERKICAIGVRCS
                     RWITMHGFALNVNTDLTWFRDIVPCGIQNKQVTSLASELGRPVTMEEVKSAWKYFFEE
                     VFHCGLTEKREVPKTLWE"
     gene            complement(1230..>2158)
                     /locus_tag="VKU83_07910"
     CDS             complement(1230..>2158)
                     /locus_tag="VKU83_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93519.1"
                     /translation="NLGILIICLFLSGLLNAQDTLPRFSAIVRGPGKVLISWHNRYPV
                     VSQISIQRSADSLRNFTTLLTVPDPALPENGAVDSRSPQPGLFYRLFIVFDNGRYLFT
                     PSRRPAADRAAKVTKVVEREDEAADKLLARAADNRIVFVDPDREHGHAVIKTPSRIHS
                     APQISDLGSSVPVFVRKGSSVIERISARQIPAFRDSILNRTRDTLVFVGGDTMLIKPF
                     VAPEVYRVSAYVYTGRYGNVHVSLPDASRRHYSVRFFDDNNKLLFELAEIRDPALIVD
                     KTNFQHSGWFRFELFDGDQLKERNKLFIPKEF"
BASE COUNT          467 a          563 c          644 g          484 t
ORIGIN      
        1 tggtgttctt gtcgatccgg tgtacgaggc cgaaccgcgg gagcgtatct tcgctgaggg
       61 cggcgttctt ctgccggaga taccacgcga caccattgat caatgtgccc gtgtagttgc
      121 cgatcgccgg atggacgacg atgcccgcgg gcttgttgat gatcatcacc tcctcatctt
      181 cataccggat gttgaggggt agcggctcgg ggatgatttc ctctccatgg gtttccttgt
      241 ccgagtaaag gacgatctca tccagcgggc gaatcttgta attggacgga acctgtttgt
      301 tgttaaccag caccatccct gcctccagcg ccttctggat cttattcctc gaggcatttt
      361 cgatccgctg aaccaggaac ttgtcgatgc gcaaggggtc ctggcctctg tccaccttca
      421 ggcagagccg ctcgtataac tcttcggacc cgtcggcagc cggagcttct tcctgcctgt
      481 cctgctgtcg ttccttgtgc atatcgattt ttttgtaaag ttattatctt tacggcttat
      541 ggagactgtg gtctttcaag atcttggcag gatggaatat caggcggcct gggactatca
      601 ggaagcattg ctgcaggaaa gcctgcgcct caaggcctca ggccgcgaca cgggccatta
      661 tctcttgttc gtcgagcatc cgcccgtctt tacgcttggc aaaagcgggc atcccgaaaa
      721 tgtgctgata ggcgaggagc ggatgcggga gaaaggggtc caattctttc ggaccaacag
      781 gggtggcgat attaccttcc atgggccggg gcaactggtg ggttatccga tcctggacct
      841 cgaaaaattc tatacggata tcggccggta cttgcgggac ctcgaggagg tgacgatccg
      901 tgtgttggcc gcctatggca tctgtgggga ccgctcaaag ggcgagaccg gggtctggat
      961 cgagcctggc gtaaaaggaa gggagcgaaa aatctgcgcg atcggagtcc ggtgcagccg
     1021 atggatcacg atgcatgggt ttgcgttaaa cgtcaatacc gacctgacct ggtttcggga
     1081 catcgtcccc tgcggcattc aaaacaaaca ggtcacctcc cttgcaagcg agcttggccg
     1141 gccggtgacc atggaggagg taaagtcggc gtggaaatac ttcttcgagg aggtattcca
     1201 ctgcgggctg accgaaaaac gcgaagtgcc taaaactctt tgggaataaa aagcttattt
     1261 ctttctttca gctgatcacc gtcaaagagc tcaaagcgaa accacccgga atgctgaaag
     1321 ttggttttat caacgataag cgcgggatcc ctgatctccg ccagttcaaa caaaagcttg
     1381 ttgttatcgt caaagaaccg gacagaatag tggcggcgtg atgcgtcagg cagcgaaacg
     1441 tggacatttc catacctgcc ggtatacaca taggcggaga ccctgtagac ttccggggcg
     1501 acaaagggct tgatgagcat ggtatcgccg cccacaaaaa cgagcgtgtc ccgcgtccgg
     1561 ttgaggatgg aatcgcggaa tgccgggatc tgtctggccg agattctttc gatcacactt
     1621 gaacccttcc gcacaaagac agggacggat gacccgaggt cagagatctg cggggcggaa
     1681 tgaatcctgg aaggggtttt gatgaccgcg tgcccgtgct cccggtccgg gtccacaaaa
     1741 acaatccggt tgtcggccgc tcttgcgagc agcttgtcgg ccgcttcgtc ttccctttca
     1801 acgaccttcg tcaccttagc cgcgcgatcc gcggcggggc ggcgtgaagg cgtgaagagg
     1861 tatcggccat tgtcgaatac gataaaaagg cgatagaaca agccaggctg cggggagcgg
     1921 ctgtcaaccg cgccattttc gggcagagct gggtccggga cggtcagcag ggtggtaaaa
     1981 tttctcaggc tgtcggctga ccgttggatg ctgatctggc tgactaccgg gtaacggtta
     2041 tgccagctga tgagcacctt gccgggaccc ctcacgatcg cggagaaccg aggcagcgta
     2101 tcctgcgcgt tgagcagacc tgagagaaaa aggcaaataa tcaggattcc aagattcc
//
LOCUS       DATGKE010000458         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_414489, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000458 DATGKE010000000
VERSION     DATGKE010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414489"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1049)
                     /locus_tag="VKU83_07915"
     CDS             complement(54..1049)
                     /locus_tag="VKU83_07915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HLI93520.1"
                     /translation="MEFQLPIQIATLPRPIGYGDKILLAGSCFTEHIGNALRDWKFNT
                     LTNPNGILFDPASVSSSLQSYLAPRYYAEDDLVFFNELWQSWQHHSIFSHPDRTECLR
                     RINASQQTAHDFLRQAGWLIITLGSAFSYRLAAGGAAVANCHRAPAQTFSRHFMTIEE
                     IMAGLDACLHRLFYFNPDLQIIFTVSPVRHIRDGVIDNNRSKARLIESVHQLAGKFDR
                     VYYFPAYELVIDVLRDYRFYDIDLVHPNYAATAFVLEKFVRHCIDEPGRSLLEEVGKL
                     VAARRHKPFHPTTQAHRRFLREQWQKATELAERYPFLDLSEELDYFSGGAINFGS"
     gene            complement(1055..1912)
                     /locus_tag="VKU83_07920"
     CDS             complement(1055..1912)
                     /locus_tag="VKU83_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HLI93521.1"
                     /translation="MNKPVTLIECPRDAMQGWPRIIPTEKKTEYLNALLRVGFDTLDF
                     GSYVSARAIPQMADTAEVLKGLQRKGSPTKLLAIVANFRGAEEAAAQDAIEYLGFPFS
                     VSETFQLRNANKTIDGSFATVEEIQELCAARQKELVVYLSMAFGNPYGDPWNEEVVFE
                     WASALARLGVRTLSVADTVGLATPGQVFDIVDFLTRQLPQCTIGVHLHSTHSNRAAKI
                     DAALDAGCRRFDGALKGIGGCPMAGDALVGNMDTEWMIRHFRGKQLMGPLDEDALQAA
                     SRIAEEIFI"
     gene            complement(1909..>2183)
                     /locus_tag="VKU83_07925"
     CDS             complement(1909..>2183)
                     /locus_tag="VKU83_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HLI93522.1"
                     /translation="DSLLKAVDTLHLNGIRVFTSEMNAVKKVFQLPLGEPCCIVMGSE
                     DQGVQPYLRKAADEHFTIPMAGDFDSFNVSVAAGIILYEAMKHRLP"
BASE COUNT          463 a          635 c          639 g          446 t
ORIGIN      
        1 aagcttccgg taaaagtcca ggattgccgc cgcctgtgtg gggcgcgctt cattcatgag
       61 ccaaagttaa tggccccacc ggaaaaataa tccagctctt cgctgaggtc aagaaacgga
      121 tatcgctctg cgagctcggt cgccttttgc cactgctccc tgagaaaacg gcggtgcgcc
      181 tgcgtcgtcg ggtgaaaagg tttgtgtcgg cgcgccgcga cgagcttccc gacctcttcc
      241 agcaagctcc tgccgggctc atcgatacaa tgccggacga atttctccag cacaaacgcg
      301 gtggcggcat agttggggtg aaccaggtcg atatcataaa aacgatagtc gcgcagcacg
      361 tctatcacca gctcataagc gggaaaatag tacacgcggt cgaacttgcc ggccagctgg
      421 tggaccgatt cgatcagcct ggccttgctc cggttattgt cgatcacgcc gtcgcggata
      481 tgccgcacgg gactaacggt aaaaattatc tgcaagtccg gattgaaata aaaaagccgg
      541 tgcaggcagg catccagacc cgccatgatc tcctcgatcg tcatgaagtg ccgggagaag
      601 gtctgtgccg gcgcccgatg gcaattggcc acggccgcgc ctcccgccgc caggcggtag
      661 gaaaacgcgc tccccagcgt gatgatcagc catcccgcct gacgaagaaa gtcatgtgcc
      721 gtttgctgtg aggcgttgat ccgccgcagg cactccgtcc ggtcgggatg ggagaagatg
      781 ctgtgatgct gccagctctg ccacagctca ttgaagaata ccaggtcatc ctccgcgtaa
      841 taccgcggcg cgagatacga ctggaggctg gaggaaacgc tggcggggtc gaagaggatg
      901 ccgttggggt tcgtcaacgt gttgaacttc caatccctga gcgcgttgcc aatatgttcg
      961 gtaaagcagg agcccgctag caggatcttg tcgccatatc cgatgggcct cggaagcgtt
     1021 gctatttgaa tgggaagttg gaattccatg ttggctagat aaagatttct tccgctattc
     1081 ggcttgccgc ctgcagggcg tcctcgtcca ggggacccat tagctgtttg ccgcgaaagt
     1141 ggcggatcat ccactccgta tccatattgc cgaccagcgc atccccggcc atcgggcagc
     1201 cgccgatgcc cttcagggcg ccgtcaaaac gccggcaacc cgcatccagg gccgcgtcga
     1261 tcttggccgc acggttagag tgcgtcgaat gcaggtggac gcctatcgtg cattgcggaa
     1321 gctgccgggt gaggaagtca acgatgtcga agacctgccc cggtgtcgcc aggccgacgg
     1381 tatcggctac cgatagggtg cgcacgccca gccgggcgag cgccgaagcc cactcgaata
     1441 cgacttcctc gttccagggg tcgccgtagg gattgccaaa agccatcgac aagtaaacca
     1501 ccagttcttt ctggcgggcc gcgcaaagct cctggatctc ttccaccgtg gcaaaggagc
     1561 catcgatggt tttattggcg ttgcgcagct gaaacgtctc gcttaccgag aaggggaagc
     1621 cgaggtactc gatggcatcc tgggcggccg cttcctccgc gccgcggaaa ttggccacga
     1681 tcgccagcag ttttgtcggc gaacctttcc gctgcagacc cttcaatacc tcagccgtgt
     1741 ccgccatctg cggaatggcg cgggctgaaa catagctgcc gaagtcgagc gtatcgaatc
     1801 ccacccgcag caaggcattc aggtactccg ttttcttttc ggtcgggata atgcgcggcc
     1861 agccttgcat ggcatcacgg gggcactcga tcaaggtcac cggcttattc atggtaatcg
     1921 atgtttcatg gcttcatata aaataatccc ggcggctacc gagacgttga atgaatcgaa
     1981 gtcgccggcc atgggaatgg tgaagtgctc gtccgcggcc ttccgcaagt aaggctgaac
     2041 accctgatcc tcgctgccca tgacgataca acagggttcg ccaagcggaa gttgaaaaac
     2101 ctttttcacc gcattcattt cactggtgaa aacacggatc ccattcaggt gcagggtgtc
     2161 cacggctttc agcaggctgt cga
//
LOCUS       DATGKE010000459         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1382552, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000459 DATGKE010000000
VERSION     DATGKE010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382552"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1504
                     /gene="leuC"
                     /locus_tag="VKU83_07930"
     CDS             134..1504
                     /gene="leuC"
                     /locus_tag="VKU83_07930"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLI93523.1"
                     /translation="MAKTLFEKIWDRHIVRSIEGGPSVLYIDKHFIHEVTSPQAFQGL
                     RNRGVEVFRPSQIVATADHNVPTVNQHLPIKEELSRIQVQQLVENCQKNHIELYGLGH
                     PYQGIVHVIGPELGVTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLATQCLLQ
                     SKPRLMRINVEGRLNKGVVSKDIILYIISRISASGATGYFVEYAGSAIRGLSMEARMT
                     ICNMSIEMGARGGMIAPDETTFSYLKGRPFAPKGAEWNRKLAWWKTLQTDADAIFDKE
                     IHIDAADIEPMITYGTNPGMGVGITGRIPATADPKSLAYMGLQPGAAIKGKKIDYVFI
                     GSCTNSRIEDLRQVASFVKGRRKADNVEVWIVPGSKQVEQQAIKEGIDKVFEAAGFHL
                     RQPGCSACLGMNEDKIPAGKYCISTSNRNFEGRQGPNARTLLASPLTAAAAAITGEVT
                     DIREFI"
     gene            1518..2138
                     /gene="leuD"
                     /locus_tag="VKU83_07935"
     CDS             1518..2138
                     /gene="leuD"
                     /locus_tag="VKU83_07935"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLI93524.1"
                     /translation="MSKAIHIIHSTAVPIELENIDTDQIIPARFLKATSREGFGDNLF
                     RDWRYNSDNTPKKDFVLNDPTYHGKILVAGRNFGCGSSREHAAWAIKDAGFDVVISCF
                     FADIFKNNALNNFLLPVTVSETFLKKIFAAVRKDPATTITVDLPAQTISLRQGGGASE
                     APGETESFEINHYKKTCLLNGYDDIDFLLSIRGEIEDFEKKLAHHE"
     gene            2166..3281
                     /gene="leuB"
                     /locus_tag="VKU83_07940"
     CDS             2166..3281
                     /gene="leuB"
                     /locus_tag="VKU83_07940"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLI93525.1"
                     /translation="MQKNIVVIEGDGIGPEVTRQAIKVLNAVAANGGHEFSYRYCLMG
                     ADAIDKTGNPLPDETIEAALNSDAILFGAIGHPKYDNDPTARVRPEQGLLKLRKSLQL
                     FANIRPVSTYPALQHLSPLKAKVLENVDFIIFRELTGGIYFGKKETDPDGNRASDECI
                     YSREEIERIAYLAFRQAQQRRKKLTLVDKANVLETSRLWRKVVQEIAAGYPDVKVDFL
                     FVDNAAMQIIINPRQFDVILTENMFGDILSDEASVISGSLGLLPSASIGKGAALFEPI
                     HGSYPQAAGKDIANPLGSILSAAMLLDHFGLHAEAARVREAANWTLQNSFVTKDIDPI
                     NFYFTSTIGELIADYVGGKIPGDIHKGNIELRKSTII"
BASE COUNT          858 a          979 c          919 g          682 t
ORIGIN      
        1 tttaaccgta acgacatcga taccatttac caacggttcc tgcaggtggc tgacacgaag
       61 aaggaagtcg tggaggaaga ccttcatcag ctcgccgctg cttacaccga gacggtaata
      121 ccatcttaat actatggcaa agacattgtt tgaaaagatc tgggaccgcc acatcgtccg
      181 ctcgatcgaa ggcggccctt ccgtgctcta tatcgacaaa cactttatcc atgaggtcac
      241 gagcccgcag gcgttccagg gactcaggaa ccggggcgta gaagtttttc gcccatcaca
      301 gatcgtcgca actgccgatc acaacgttcc gaccgtcaac cagcatctgc ccatcaagga
      361 ggaattgtcc cgcatccagg tgcaacagct cgtcgaaaat tgccaaaaga atcatattga
      421 gttatacggt ctcggccacc cttaccaggg catcgtgcat gtcatcgggc ccgaactggg
      481 tgtcacgcaa cctggcatga ccatcgtatg cggtgacagt cacacttcca cccacggcgc
      541 cttcggggca atcgccttcg gcatcggaac gtcggaggtg gaaatggtcc tggctaccca
      601 gtgcctgctg cagagcaagc ccagactgat gcgcatcaat gtggagggaa ggctgaacaa
      661 gggcgtggta tcgaaagata ttatcctgta tattatttcc cggatctccg ccagcggtgc
      721 tacgggctat tttgtcgaat acgcgggctc ggcgatccgc ggcctttcca tggaagcccg
      781 gatgaccatc tgtaatatga gcatcgagat gggagctcgc ggcggcatga tcgcgcccga
      841 cgaaaccacc ttcagctacc tgaagggccg gccatttgcc cccaaaggcg ctgaatggaa
      901 ccggaaactg gcctggtgga agaccctgca aacagacgct gacgccatat tcgacaagga
      961 gatccatatc gacgcagccg atatcgagcc aatgatcact tacgggacga acccgggtat
     1021 gggcgtcggc atcaccggtc gcatcccggc aaccgccgac cctaagtcgc tcgcttacat
     1081 gggcctgcag cccggcgccg ccattaaagg aaagaagatc gattacgtat ttattggcag
     1141 ttgcacgaac tcccggatcg aggaccttcg gcaggtcgcc tccttcgtca aaggccgcag
     1201 gaaagcggat aacgtcgagg tttggattgt ccctggaagc aagcaggtag agcaacaagc
     1261 gatcaaggaa ggtatcgaca aggtttttga ggcggcgggc ttccacctcc ggcagccggg
     1321 ctgcagcgcc tgcctgggaa tgaacgagga taagatccct gccggtaaat attgcatttc
     1381 cacctcgaac cggaattttg aggggaggca ggggcccaat gcacggacct tgcttgccag
     1441 cccgttgacc gcggcagcag cagccatcac cggagaagtt accgatatcc gggaattcat
     1501 ctgatcaaaa acacgtcatg agcaaggcca tccatatcat tcattcgaca gccgtcccga
     1561 tagaattgga aaacatcgat accgaccaga tcatccctgc ccgcttcctc aaggcgacca
     1621 gccgcgaggg ctttggcgat aatctcttca gggactggcg ctataacagt gataataccc
     1681 ccaaaaagga ttttgtcctc aatgatccta cgtatcacgg gaagatcctt gtcgcgggaa
     1741 gaaatttcgg ctgcggatcc agccgcgagc acgccgcctg ggccattaag gacgccggat
     1801 tcgatgtcgt cataagctgt ttttttgccg acatcttcaa gaataacgcg ctgaataatt
     1861 tcctgctgcc ggtaaccgtc agcgaaacct tcctcaaaaa gatatttgct gcggtaagga
     1921 aggatcccgc tacaacgatc accgtcgacc ttccggctca gaccatcagc ctccggcaag
     1981 gcggcggggc atccgaagcg ccaggcgaaa cggaatcctt tgagatcaat cattacaaga
     2041 aaacctgttt actcaacgga tatgacgata tcgacttcct gctcagcatc cgcggggaga
     2101 tcgaagattt tgaaaagaag ctcgcccatc atgaataaca gctctaccct aaatatcgat
     2161 tgtatatgca aaaaaacatt gtagttattg aaggcgacgg catcggcccg gaggtgaccc
     2221 ggcaggcgat caaagtgctg aacgccgtgg ccgccaatgg tggccatgaa ttttcataca
     2281 ggtattgcct gatgggcgcc gatgccatcg acaagaccgg taacccgttg cccgacgaaa
     2341 cgatcgaagc cgcactgaac agcgacgcca tcctgttcgg cgcgatcggc cacccgaagt
     2401 atgataacga cccgaccgcc agggtccgcc cggaacaggg tttgctgaaa ctgagaaaat
     2461 ccctgcagct gttcgccaac atccgccccg tttccaccta ccccgccctc caacacctgt
     2521 cgccgctgaa agcgaaagtg ctggagaacg tggacttcat catcttccgg gaactgaccg
     2581 gaggcatcta tttcggaaag aaagagactg acccggacgg caaccgcgcg tcggatgaat
     2641 gcatttactc gcgcgaagag atcgaacgca ttgcctatct ggcgttcagg caggcacagc
     2701 agcggcgtaa aaaactcacg cttgtcgaca aggccaacgt cctcgagacc tcgcgccttt
     2761 ggcgaaaggt agtccaggag atcgccgccg gttatccgga tgtaaaggtg gactttctct
     2821 tcgtcgataa cgccgcgatg caaatcatca tcaatccaag gcaattcgac gttattctga
     2881 ccgaaaacat gttcggcgat atcctcagcg atgaggccag cgtcatcagc ggttcgctcg
     2941 ggcttcttcc ctcggcctcg atcgggaaag gggctgcctt gttcgagccc atccatggct
     3001 cgtacccgca ggccgcgggc aaggacatcg cgaaccccct tggcagcatc ctctccgcgg
     3061 ccatgctcct cgatcacttc ggtttgcatg cagaagccgc aagggttcgg gaagcggcga
     3121 actggacgct gcaaaacagc ttcgttacga aggatatcga ccctatcaat ttctatttca
     3181 cgagcaccat cggagagctg atcgcagatt acgtcggcgg caagatcccc ggcgatatcc
     3241 ataagggaaa catcgagttg aggaaatcga cgatcattta agaaaatcgc ggaagcgcga
     3301 agcgagatat atggcacaat actataacga aaacacggtt ctctggctgg atggccagtt
     3361 gctgaaggcg tcggagacga agaccgacct gtatgggcaa tccctgcatt atggctacgc
     3421 cgtcttcgag ggcatccg
//
LOCUS       DATGKE010000460         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_942594, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000460 DATGKE010000000
VERSION     DATGKE010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942594"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VKU83_07945"
     CDS             <1..709
                     /locus_tag="VKU83_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLI93526.1"
                     /translation="NLLLNDYLAREFDFPQSAYFTVMGPCHAEEIAAEKLSYLTFSGQ
                     DETGAAELASYFTTEYLNTVINADVYGVQYAAILKNIYALGAGIAHGLEYGDNFLSVL
                     IANCADEMAGFLRKVGIRHIEVGVHAETADAANNHRRSANYAASVYLGDLLVTCYSLY
                     SRNRMFGSMIGKGYSVKAAQLELNMVAEGYNASKCIYLINKDIKAEMPIAGMIYRILW
                     ENVKAGPGFKQIEPYLV"
     gene            complement(884..2044)
                     /gene="hemW"
                     /locus_tag="VKU83_07950"
     CDS             complement(884..2044)
                     /gene="hemW"
                     /locus_tag="VKU83_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLI93527.1"
                     /translation="MAGVYIHIPFCRQACHYCNFHFSTSMRGRNDFIPALLKEMESRT
                     DYLGGERVETIYFGGGTPSLLEPGELQTILERVRALFPVDAEAEATLEANPDDITSSR
                     LDAWRSAGINRLSIGVQSFYDEDLRWMNRAHDARQALDCIRLAKAAGIHNLSIDLIYG
                     GPTLADNHWRRNVADAIALGVSHLSCYALTVEPRTALDKLIRRKAVTDVSPDDQARQF
                     LLLMDWLGKAGYEHYEISNFALPGRRSRHNSSYWQGKPYLGLGPSAHSFNGLSRQWNV
                     ANNALYIAALNGQRPEQPGVGLPAGLVEGKEELTPVQQLNEYILISLRTMEGSSHQTV
                     ARRFGEQQATALWQKAATYIRSGKIGDSAEVLRLTPAGKLLADGIAADLFFE"
     gene            2063..>3009
                     /locus_tag="VKU83_07955"
     CDS             2063..>3009
                     /locus_tag="VKU83_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93528.1"
                     /translation="MPRIAPCQMTFMALRRYVIVGLWPLLLLPFSGWTQYALHILPVD
                     RDSVFIRSRLGLTTNFRSQEACTAYIDNLIPLLQSKGYITASADSIGYTAKEATLRLF
                     VGAAWRWGYIDTRRVDPGLLAAVAWNPRTFAHRPLDFRVFQARQRQLLNYMENNGYPF
                     ARISLDSIVIRDSAEVAAVLRIDKGPLYHIDSIRIYGPAKISNEFLQRYLNIPNGSIY
                     RKDRLGAISKKLLELPYIQEQQPWSLSMLGEGSVVNLYLKPKKSSQINALVGFLPSSD
                     PVLGNKLLVTGEATINLQNALGNGETMALNWQQLQQNSPR"
BASE COUNT          656 a          912 c          809 g          632 t
ORIGIN      
        1 gaatctgctc ctgaatgact acctcgcccg ggagttcgat tttccgcagt cggcttattt
       61 tacggtgatg ggtccctgtc atgcggagga aatagccgcg gaaaagctct cctacctgac
      121 tttttccggc caggatgaaa ccggggccgc ggaactggcg tcttatttta cgacggaata
      181 cctgaatacg gtgatcaacg cggatgtcta tggcgtacaa tatgccgcca tcctcaagaa
      241 catctacgcc ctgggcgccg gtatcgcgca tggcctcgaa tacggcgaca atttcttgag
      301 cgtcctcatc gccaactgcg cggatgaaat ggccggcttc cttcggaaag taggcatccg
      361 ccatatcgaa gtaggggtcc atgcagaaac ggcggacgct gcgaacaatc atagaaggag
      421 cgccaactat gctgcatcgg tctacctcgg cgatctgctg gtgacctgct attcgctgta
      481 tagccgcaac cggatgttcg gcagtatgat cggtaaagga tactccgtca aggccgccca
      541 gctggaactg aatatggtcg cggagggtta taacgccagc aagtgtatct acctgataaa
      601 caaggatata aaggcggaga tgcccattgc cggtatgatc taccggatct tatgggaaaa
      661 tgtgaaggcc ggtcctggat ttaaacaaat tgagccgtat cttgtctaaa gaataaaagc
      721 attcaatatg cccttctcct tccaaggctt cggaaccatg gccatcctcg ggatggtgct
      781 tatcctggcg gcagcgcagg gcatcgagta tctgcgatcc cgccaccggc atcactgagg
      841 ccgcggccgg catcgatgag cccgcctgca tcatagacga caatcactcg aagaacagat
      901 ccgctgcaat cccatcggcc agcaatttgc cagctggggt caatcgtaac acttccgccg
      961 aatccccgat ctttccggat cgtatgtacg ttgccgcttt ctgccaaagc gctgtggcct
     1021 gctgttcccc gaaccgccgg gcaacggttt gatgactgct gccttccatg gtgcgcaggg
     1081 agatcaaaat gtattcatta agctgctgca cgggcgttag ctcttcttta ccttccacca
     1141 ggccggctgg aagcccgact cccggctgtt ccggccgctg gccgttcagc gcggcaatat
     1201 aaagcgcatt gttggccaca ttccattgcc gcgacaggcc gttgaaagaa tgcgccgatg
     1261 ggccgagtcc caaatacggc tttccctgcc aataggagga gttgtgccgg ctccggcggc
     1321 ccggcagcgc aaaattggag atctcgtagt gttcatagcc tgccttgccc agccagtcca
     1381 tcagcagcag gaattgtctg gcctggtcgt ccggactgac atcggtcacc gctttccggc
     1441 ggatcagctt gtcgagcgcc gtcctgggct caaccgtaag cgcatagcag gacagatggg
     1501 agacgcccag ggcgatggcg tccgctacat tccggcgcca atgattgtcg gcgagggtag
     1561 ggcctccata gatcagatcg atgcttaggt tgtggatccc cgccgctttc gccaggcgga
     1621 tacagtccag cgcctggcgg gcatcatggg cccggttcat ccaccggagg tcttcatcgt
     1681 aaaagctctg aacgccgatg ctcaggcggt tgatccccgc ggaacgccag gcatccagac
     1741 gggaagaagt aatatcatcc ggattcgcct cgagggtggc ctccgcttcc gcatctaccg
     1801 ggaataacgc cctcacccgc tccaggatgg tttgcagctc tcctggttcc agcagcgaag
     1861 gcgtcccgcc gccaaaataa atagtctcca cccgctcccc gcccagataa tcggttctgc
     1921 tctccatttc cttcaataaa gccgggataa aatcgtttct acctctcatg gacgtggaaa
     1981 aatggaaatt gcagtaatga caggcttgcc tgcaaaaggg gatatggata taaacgcctg
     2041 ccatccggta aaattacgca aattgccgcg catcgcaccc tgtcagatga ccttcatggc
     2101 gctacggcga tacgtcattg taggactgtg gcccttgctg ctcctcccat tcagcggctg
     2161 gacccaatat gccctgcaca tcctccctgt cgacagggac tccgttttca ttcgcagccg
     2221 gttgggtctt accacgaact tccggtcgca ggaggcctgc acggcctaca tcgataacct
     2281 gatcccgctc cttcagtcca aaggctacat cactgcttcc gccgatagta tcggctatac
     2341 cgcaaaagag gccaccctcc ggctttttgt cggggcagcc tggcgatggg gatatatcga
     2401 cacccgccgt gtagatcccg gcctgctggc cgccgtggca tggaacccca ggaccttcgc
     2461 gcatcgcccc ctcgatttcc gcgttttcca ggcccgtcag cggcagctcc tgaactatat
     2521 ggagaacaac ggctatccgt tcgcccgcat atccctggac agcatcgtca tccgggactc
     2581 ggccgaagta gctgcggtcc tccggatcga caagggacca ttgtaccata tcgatagtat
     2641 ccgcatctac ggaccggcaa agatctccaa cgagttcctg cagcgctacc tgaacatccc
     2701 caacggcagc atctaccgga aggaccggct cggagcgatc agcaagaagc tgctggagct
     2761 gccctacatc caggaacagc agccctggag cctttcgatg ctgggcgagg gctctgtcgt
     2821 caatctctac ctcaaaccga aaaagagcag tcagatcaac gccctggtgg gctttcttcc
     2881 cagcagcgac cccgtactgg gaaacaagct gctcgttacc ggcgaggcga ccatcaatct
     2941 gcagaatgca ctgggcaacg gcgagaccat ggccctaaac tggcagcagc tgcaacagaa
     3001 ctctccgcg
//
LOCUS       DATGKE010000461         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1415599, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000461 DATGKE010000000
VERSION     DATGKE010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415599"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..893
                     /locus_tag="VKU83_07960"
     CDS             210..893
                     /locus_tag="VKU83_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93529.1"
                     /translation="MYGSSGNSYLVSETDMNSAPSLHAVLTADIVHFTRLGDEAADAL
                     LQSLQRELLPYQVEFYRGDSFQAYMEKPEGSLRLALLCRALAISLPAVEHGITGSDIR
                     ISIGLGPVIQPVQAPGIAKGDAFIRSGRGLDAIQGTDRRIAIISGHAIADIGLEAMAN
                     HLDDIYRHMTAKQAAAIVWLLRGVTQQEATAQLSKSKSTVSQLVNAGNWGQIEEILKL
                     FQSLIKQLS"
     gene            890..1600
                     /locus_tag="VKU83_07965"
     CDS             890..1600
                     /locus_tag="VKU83_07965"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HLI93530.1"
                     /translation="MIPGSVWLTKLLIAHLLTDFVFQPPGWIEDRQRKHLASVKLGLH
                     AVLTAIVAYLFIGLKYWPVALVILFTHWLVDGWKSYRPQDVRYFLADQFLHLAIIGGC
                     WWFSFPDHRPLEAVVSNWGSDAHNWIVTAGFVFVAYPAGVLIGQLTGQWSRKIDDDKQ
                     SLANAGKWIGIAERVIVLVLVLQNQYSAIGLLITAKGIIRFSEKDRQEVKTEYLVIGT
                     LLSIGVAILAGLAIKGLS"
     gene            1627..>2216
                     /locus_tag="VKU83_07970"
     CDS             1627..>2216
                     /locus_tag="VKU83_07970"
                     /inference="COORDINATES: protein motif:HMM:NF039197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HLI93531.1"
                     /translation="MLISRQYFKARSILLCLLSISFFSRAQDSTVIPAQKIKVYLVGT
                     FHFDGAAGDVYESARQDMKTAANQQQLDDLARKLARATPDKIFVEWNPDRQGYADTTY
                     QLYLRNQFDLGNSEVYQVGYRLGKMLGHQRIYCVDAGGEVDYDSVVGDAAQYHQSEIL
                     SDLNHPKDSVAKLISKRIASIYSYYRTPQVRTLTDV"
BASE COUNT          533 a          559 c          616 g          508 t
ORIGIN      
        1 tcccggtcag gggctcgggt ggcgcccgat tcttcgcagg cggtggcaaa gcccaaggaa
       61 gtgcttgaat ttgaaaaaaa agcggccaag cataaaatta aagtgcagaa cgggagcact
      121 tatcaataga cccccgttac ggttcggtat atcttacgga attaaaattg gttcggttaa
      181 atcagcggaa aatattctag ttcgtcaaaa tgtacggaag cagtgggaat tcgtatcttg
      241 tgtctgaaac cgatatgaac tccgctcctt cactacatgc cgtcctgacg gccgatatcg
      301 tccatttcac ccggcttggg gacgaagcag cagacgcgct cctgcagtcg ctacagcgcg
      361 aattattgcc ttaccaggtc gaattttacc ggggcgacag cttccaggct tatatggaaa
      421 aaccggaagg ctcattgcgg ctggcactgc tctgccgggc tctggcgatc agcctgccgg
      481 cggtggaaca tgggataacg ggatcggata ttcgaatcag cattggtctg gggccggtta
      541 tacagccggt gcaggcgccg gggatcgcaa aaggcgatgc gtttatcagg tcgggccggg
      601 gcctggacgc gatccagggc accgacaggc gcattgcgat catcagcggc catgccatcg
      661 cagacatcgg cctggaggca atggccaacc atctcgatga tatttaccgg cacatgacgg
      721 ccaaacaggc agctgccata gtatggctgc taagaggggt cacacagcag gaagcgacgg
      781 cgcaactcag taagtccaag agcaccgttt cccagttggt caatgccggc aactggggcc
      841 agatcgaaga gatattgaaa ctgttccaat ccttgatcaa acagctctca tgataccagg
      901 atccgtttgg ctaaccaagt tactgatagc gcacttattg actgacttcg tttttcagcc
      961 gcccggctgg atcgaagacc ggcaacgaaa acatttggcg tccgtaaaat tgggcttgca
     1021 tgctgtactg acagcaatag tcgcttatct tttcataggc ttgaaatact ggcccgtggc
     1081 gctggttatc ctcttcacgc actggctggt ggatggctgg aaatcgtacc ggccgcaaga
     1141 cgtccggtac ttccttgccg accaattcct tcacctggca attatcggag gatgctggtg
     1201 gttcagtttt cccgatcatc gtcccctgga agcggttgtc agcaattggg gttcggacgc
     1261 gcacaactgg atcgtcacgg cgggatttgt gtttgtcgct tatccggcag gcgtactcat
     1321 cggacaattg accggacaat ggagtaggaa gatagatgac gataaacaaa gccttgcgaa
     1381 cgcgggtaag tggatcggca ttgccgagcg cgtcatcgtg ctcgtcctgg tccttcaaaa
     1441 ccagtattcg gctatcggct tgctgattac ggcgaagggg attatccgtt ttagtgaaaa
     1501 ggaccggcag gaggtaaaga ccgagtacct cgtgataggt acgctgctga gcatcggggt
     1561 cgcgatcctc gcaggtttgg cgataaaagg actgagctga tctcctataa ttttgtaaat
     1621 tggctgatgc taatctctcg ccaatatttt aaggcccgat cgatcctcct ctgtcttctg
     1681 tccatctcct tcttttcacg cgcacaggat tcgaccgtca tacccgcgca aaaaatcaaa
     1741 gtatatcttg tcggtacctt tcactttgac ggcgctgcgg gagatgtcta cgagtcggcg
     1801 cggcaggaca tgaaaacggc ggcaaaccag caacagctgg atgaccttgc gcggaagctc
     1861 gccagggcaa caccggacaa gatctttgta gaatggaatc ccgaccggca gggatatgcg
     1921 gacacaacgt atcaattgta tttgcgaaac caattcgacc ttgggaacag cgaggtatac
     1981 caggtcgggt atcggttggg gaaaatgctg gggcaccaac gcatttactg cgtggatgca
     2041 ggtggcgagg tggactacga ttccgtagtc ggggacgcgg cgcagtatca tcaatcggag
     2101 atactcagcg atctgaatca tccgaaagac agtgttgcaa aactcatctc caaacggatt
     2161 gcctcaattt attcctatta ccgcactccg caggttcgca cgctcactga tgttat
//
LOCUS       DATGKE010000462         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_129432, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000462 DATGKE010000000
VERSION     DATGKE010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129432"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2135
                     /locus_tag="VKU83_07975"
     CDS             <1..>2135
                     /locus_tag="VKU83_07975"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HLI93532.1"
                     /translation="PDYLQYYRARLLKQMGANAVRTSHNPPTPEWLDACDSLGLLVLD
                     ETRLLNSGPEYLSQFKRLIFRDRSRASVFLWSIGNEEGYVQTQPVGKDIALTMIARQK
                     EWDPTRTCTYAADLANVFQGIDQVIPVRGFNYRIKGVDPYHKDHPGQPVIGTEQGSTV
                     TTRGIYEKDSIRCYLPDEDITAPWWANTAEQWWSLCADRPWWAGGFVWTGFDYRGEPT
                     PFQWPNINSHFGVMDMCGFPKNIYYYYRSWWTDEDVLHISPHWNWKGKEGQAIPVWVN
                     SNADDVELFLNGKSLGKKVMPRNGHLEWAVPYEPGKLEAIATRKGRQFSAFVETTGPA
                     SEVVVTPYKTTMLADGRDATVLNISVVDREGREVPDAGNEIRFTAAGDLHIIGVGNGD
                     PSSHEPDKGTDSVAERRLFNGHAQVIVQSGFRPGMYHFTAASQGLYSGATDITTIVPG
                     APHSVLGWEPKDEARADRVSGRRSEGSKRGGDGAPSLNLPRARPVTKMLGADISFLPE
                     LEARGMKFSDNKGQRDAIEILKDHGFNYIRLRIFNDPARDSGYSPHKGFCDLAHTMAM
                     AKRVKAAGLKFLLDFHYSDYWADPGKQFKPAAWRGESFPGLEQSVYDWTRKVMDSLRW
                     QGTLPDMVQIGNEINHGMIWPEGSIRHPDSLAALIYAGIRGVKAIEPACPIMLHIALG
                     GQNDESHFFLDNMLERQVPFDVIGLSYYP"
BASE COUNT          461 a          572 c          688 g          414 t
ORIGIN      
        1 tgcccgatta cctgcaatat taccgggcga ggctgctgaa gcagatgggg gcgaatgccg
       61 tacggaccag tcataatcca cctacgccgg aatggctgga tgcgtgcgac agcctgggcc
      121 tgcttgttct cgatgagacc cggttgctca acagcggccc cgaatacctg agccagttca
      181 agcgcctcat cttccgggat cgcagccggg cgtcggtctt tctctggagc attggaaatg
      241 aggaaggata tgtgcagacg cagcccgtgg ggaaggatat cgcgcttacg atgatcgccc
      301 ggcaaaaaga gtgggaccct acccggactt gtacctatgc ggccgacctt gccaacgtgt
      361 ttcaggggat cgaccaggtg ataccggtga ggggttttaa ttaccggata aaaggcgtgg
      421 atccgtatca caaggaccat cccggccagc cggtcatcgg aaccgagcag ggaagtaccg
      481 tcacgacgcg cggcatctat gagaaagaca gcatccgctg ttaccttccg gatgaagata
      541 ttacggcgcc ctggtgggcc aatacggccg agcaatggtg gtcgctgtgc gcggacaggc
      601 cctggtgggc gggagggttt gtctggaccg ggtttgatta ccgtggcgag ccgaccccct
      661 ttcagtggcc caacatcaac agccattttg gggtaatgga catgtgcgga tttccgaaga
      721 atatttatta ctactaccgg tcctggtgga ccgatgaaga tgtgctccat atctcccctc
      781 actggaactg gaaagggaag gaggggcagg cgatcccggt atgggtcaac tcgaatgccg
      841 atgacgtgga gttgtttcta aacggaaaaa gcctggggaa gaaagtcatg ccgcggaacg
      901 gccacctcga atgggcggtt ccctatgagc ccggcaagtt agaagcaatc gcgaccagga
      961 agggcaggca gttctcggcg ttcgtcgaga cgacgggccc ggcgtcggaa gtggtggtga
     1021 cgccttacaa gacgacaatg cttgcagacg ggcgcgacgc gacagtcctc aatatcagcg
     1081 tggtggaccg ggaggggcgc gaggttccgg atgccggcaa cgagatccgg tttacggccg
     1141 cgggagacct gcatatcatc ggagtgggca acggcgatcc cagcagccat gagcccgaca
     1201 aaggaaccga tagtgtcgcg gagcgcaggc tttttaacgg ccatgcccag gtgatcgtgc
     1261 agtcaggatt tcggccggga atgtatcatt ttaccgctgc gtcgcagggc ttatattccg
     1321 gggcgacgga tatcacgacg atcgtgcctg gcgcgcccca ttccgtgctc ggctgggagc
     1381 cgaaggacga ggcgcgggcc gatagggttt cagggcggcg cagtgaggga tcgaagcgtg
     1441 gcggtgatgg tgcgccttcg ctaaaccttc cgcgggcaag gccagtgacg aaaatgctgg
     1501 gcgccgatat ttccttcctg ccggaactgg aggccagggg catgaaattc tcggacaaca
     1561 agggccagcg cgatgcgatc gaaattttga aggaccatgg gttcaattat atccggctcc
     1621 ggattttcaa tgatccggcg agggacagcg gctattcgcc gcacaaaggg ttctgcgatc
     1681 tggcgcacac catggcgatg gcaaaacgcg tcaaggccgc cggcctgaaa tttctgttgg
     1741 actttcacta tagtgactac tgggcggatc ctggcaagca attcaagccc gcggcgtggc
     1801 gcggcgagtc cttccccggg ctggagcagt ccgtctatga ctggaccagg aaagtgatgg
     1861 actccctgcg ctggcagggt accctacccg atatggtgca gataggcaat gagatcaacc
     1921 acgggatgat ctggcccgag ggcagcatcc gccatccgga cagcctggca gcattgatct
     1981 atgcggggat ccgcggggtc aaggccatcg aaccggcatg tcccatcatg ctgcatatcg
     2041 cactcggcgg gcaaaacgac gaatcgcatt tctttctcga caatatgctc gagcggcagg
     2101 tgccgtttga cgtcattggt ttatcgtact acccc
//
LOCUS       DATGKE010000463         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1356146, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000463 DATGKE010000000
VERSION     DATGKE010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356146"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VKU83_07980"
     CDS             complement(<1..251)
                     /locus_tag="VKU83_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HLI93533.1"
                     /translation="MIKISFPDGAVREYENGVTALEIAKSISEGLARKVLAANINGQV
                     WDATRPIHTDATLKLLTWSDAEGKSTFWHSSAHLMAEAV"
     gene            375..1520
                     /locus_tag="VKU83_07985"
     CDS             375..1520
                     /locus_tag="VKU83_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93534.1"
                     /translation="MPMKRVLLFLSFAAPLLVRGQDLTGIWQGHFRSATGERSMMTIF
                     DDRYKFEVQIAQHDRKFDAVTYSYLSNIFYGKAAAAGTVNPRTGKVLLQEGKLLELRN
                     AFGGVCIMTCFLQYTKSGNDEFLEGKYVSMDLKDSGNCGHGIVFLHKVASSDFYEEPF
                     LVERENELKKENPAKDKPTVKPEPRASATKPPLIARKMPSSRGDSTGLATARRPSPHR
                     ARQQPPTQPAAPPLARATIPRRMELRPESDSATGLSHKFPTIAPRILRDRQNQLVRAV
                     TVNTREVVLNIYDDGVIDGDTVSVYLDNRMVINHAMLTDKPLVVTIHLDEDNDYHELV
                     MVADNEGSIPPNTSLMIVKAGDKEYKVNITSSLQKNATVTFRYVKPN"
     gene            1522..2364
                     /locus_tag="VKU83_07990"
     CDS             1522..2364
                     /locus_tag="VKU83_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93535.1"
                     /translation="MRVILLAILLFAAAAGRSEDLNGIWKGTLTQGPGGCYPQYSLEL
                     QINYNDGRITGKAYDYYDKAHFVKMSFTGRYNPRTRRLVLIENQVLQAEIPIECEACI
                     KTYDLNYSTNGSEEELTGEWKGTYSANRLVCPPGQIRLKRASSSDFPVDIDQSDTLAA
                     LQKTLKLQPRQPTLVKTITLHAADVKIELYDDAEIDHDTVTVLINNKLLLYRQMLTDR
                     PLTIHFNAFPNTPYELVMYADNLGDIPPNTALMMVTCGGEKLEAYMSSSEQNSATVRF
                     IYKP"
BASE COUNT          610 a          731 c          635 g          509 t
ORIGIN      
        1 tcgaccgcct ccgccatcag gtgggccgaa gaatgccaga acgtcgactt gccttcagca
       61 tcggaccagg tgagcagttt cagggttgca tcggtatgga taggccgcgt cgcatcccaa
      121 acctgtccgt ttatgttcgc cgccaaaacc tttcgcgcca atccctcgct gatggatttg
      181 gcgatctcca atgcagtgac gccgttctca tattctctga cggcgccatc cggaaaactg
      241 attttgatca taagggtgca aatttaacaa actcttgtca aacaaagggg ttcttaactc
      301 cgcagtttca ggccgttttt tacagatggt aaagacggca agcgattaat taattaattt
      361 gccgaaagga tacaatgccc atgaaaagag ttttattgtt cctgtcattt gccgcaccat
      421 tgcttgtccg gggccaggac ctgaccggga tttggcaggg gcatttccgg tctgccacgg
      481 gcgagcggtc catgatgacc attttcgacg accgctacaa attcgaagtg cagatcgccc
      541 agcacgacag gaaattcgac gcggtcacct actcctatct gagcaatatt ttctacggca
      601 aggccgccgc agcgggtact gtcaatcccc ggaccgggaa agtgctcctg caggagggga
      661 agctgctgga actgcgcaat gcattcggcg gcgtttgtat catgacctgc ttcctgcaat
      721 acacgaagtc tggtaatgac gaattcctcg aaggcaagta cgtaagcatg gacctcaagg
      781 actcaggcaa ttgcgggcat ggcattgttt tcctgcacaa agtcgccagc tccgactttt
      841 acgaagagcc tttcctggtc gaaagggaaa atgaattgaa gaaggaaaat cccgcgaagg
      901 acaagccgac ggtgaagccc gagcctcgcg cttcggccac gaagccgccg ttgatcgccc
      961 gtaaaatgcc ttccagccgg ggcgatagta ccggcctggc tactgcccgc cggccctcac
     1021 cccaccgcgc ccgccagcag ccccctaccc aaccggccgc tccgccgctg gccagggcga
     1081 ccataccccg aagaatggag cttcgcccgg agtcggattc cgccacgggc ctgagtcaca
     1141 aattcccgac aattgctccc cggatcctcc gcgaccggca aaatcaactg gtccgggcgg
     1201 tgacagtcaa tacccgcgaa gtcgtcctca acatttacga tgacggggtc attgacggcg
     1261 ataccgtctc ggtatacctg gacaacagga tggtcatcaa ccatgccatg ctgaccgata
     1321 agccccttgt cgttacgatc catctcgatg aggacaacga ctatcacgaa ctcgtcatgg
     1381 tggcggacaa cgagggatcg attcccccca acacgtcatt gatgatcgta aaggcgggcg
     1441 acaaggaata taaagtcaac atcacttcca gcctccagaa gaacgccacc gttaccttca
     1501 gatatgtaaa accaaactga catgcgagtc attctcctgg cgatccttct cttcgctgcc
     1561 gctgccggca ggagcgaaga tctgaacggg atctggaaag gcaccctgac gcagggaccc
     1621 ggcggctgtt acccgcaata ttccctcgag ctgcagatca attataacga cgggcgcatc
     1681 acgggcaaag cttatgatta ttatgacaaa gcccattttg taaagatgag ctttacgggc
     1741 cggtacaatc cccggacgcg ccggcttgtc ctgatcgaga accaggtttt gcaggcggaa
     1801 atcccgatag agtgtgaagc ctgcatcaaa acatacgatt tgaactattc tacgaatggc
     1861 agcgaagaag agctgactgg ggaatggaag ggcacctact ccgccaaccg cctcgtatgc
     1921 ccgccggggc aaatccggct aaaacgcgcc agcagctccg atttcccggt cgatatcgat
     1981 cagtctgaca cgctggccgc gctgcaaaag acgctgaaac tgcagccccg ccagccgacg
     2041 ctggtgaaga cgatcacgct gcatgccgcc gatgtcaaga tcgaattgta tgacgatgcg
     2101 gagatcgatc acgacacggt cacggtcctt atcaataaca agctgctctt ataccgccag
     2161 atgctaacgg acaggccgct gaccattcat ttcaacgcct tccccaacac cccctatgaa
     2221 cttgtcatgt atgcggacaa cctcggcgat atcccaccga ataccgcatt gatgatggta
     2281 acctgcggcg gcgaaaagct tgaggcctac atgtcgtcca gcgaacaaaa cagtgccacc
     2341 gtgcgattca tctataagcc gtgatcacaa acacgccgct atccgccagg gcccgcctac
     2401 ttcgtcgcga aattcaggga atcggtgacc tcgccgcaac ccaccatttt agctttgtaa
     2461 acggggtgct tgtttccgag ataag
//
LOCUS       DATGKE010000464         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_323105, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000464 DATGKE010000000
VERSION     DATGKE010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323105"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VKU83_07995"
     CDS             <1..518
                     /locus_tag="VKU83_07995"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLI93536.1"
                     /translation="GEDLGMVPRCVPDVMRRLGILSMEIQRMPKNPNREFFHPAEAPY
                     LSVVTPSTHDMSTIRGWWEEDRIKTQRFFNNELGQWGEAPVFCEPWINRAIILQHLNS
                     PAQWCVFQLQDIMGMSEAFRRENPHDERINVPANPHHYWRYRMHLTLEELMDEKEFNN
                     GLRESIKASGR"
     gene            complement(581..1228)
                     /locus_tag="VKU83_08000"
     CDS             complement(581..1228)
                     /locus_tag="VKU83_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93537.1"
                     /translation="MKSKRTLFTLALAALVANTAFCQEVAEQNVTPSLLLSFTGSADG
                     SCADLAWVTENETNSRWFVVERAGADGGFDSIAVVMGINNGNTTNYSFSDENMLPGTN
                     CYRLREVDMGGVQRFSKVIRLENGQSAADRMGLYPNPAVATINYTVTASASQEVLVQI
                     YSLTGVMVSSSQQELQAGSNLETVSINNLRAGNYVLKVSSQSGSFHFVQPFVKVI"
     gene            1437..2465
                     /locus_tag="VKU83_08005"
     CDS             1437..2465
                     /locus_tag="VKU83_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HLI93538.1"
                     /translation="MTLNYYSHNLAFRHPFTISRGTKTHQPTLIVELGLGTLKGYGEA
                     PAISYYHIPVARMVADLEAKRPLIEKFALTDPERYWHYLHHLFPDNPFLVCALDMAAW
                     DLFGRLHGQPLYKLWGLNPSKAPLTDYTIGIDTVAKMVGKMQEKPWPIYKIKLGTPDD
                     IAIVSEIRKHTRSTLRVDANAGWNLAEALDKIPRLAALGVELVEQPLPRDDRQGMKAL
                     FAASSLPLIADESCVREEDVETCAGHFHGINIKLTKCSGITPARRMIAAARSLGMKVM
                     AGSMNESSVGSAAIAHLLPYLDYVDMDGPLLLSEDLGTGLEFDYGKVTIPQEPGLGVT
                     YTGVFSKP"
     gene            2503..3678
                     /gene="galK"
                     /locus_tag="VKU83_08010"
     CDS             2503..3678
                     /gene="galK"
                     /locus_tag="VKU83_08010"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HLI93539.1"
                     /translation="MSPSSLVREKYLAAFGGNPVLVRSPGRVNLIGEHTDYNHGFVLP
                     AAIDKAIYMAAGRREDREIHLMALDLQKMYRGSLDKLEASSQHWPDYLLGVVQQLLQA
                     DQVRDAGRPIGGFNIVFTGDIPLGAGMSSSAALECATAFALNELFGLDLDRMTMVRLA
                     QKAENQFVGVKCGIMDQFASMFGRRDHVIRLDCDSLAYTYFPLNLNDIRIVLLDTHVH
                     HSLASSEYNTRRRQCEAGVKLVRACHPEVASLRDVTLDMLDEYVAPIDELVYRRCRFI
                     IRENNRLLAGTEDLQKGDLESFGLKMFETHDGLSRDYEVSCKELDFLVAGVRNDPAVL
                     GARMMGGGFGGCTINLVKKGAIDNLIARIGPVYRKAMNKELTAYIARIEDGTSLMQS"
     gene            3713..4888
                     /locus_tag="VKU83_08015"
     CDS             3713..4888
                     /locus_tag="VKU83_08015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HLI93540.1"
                     /translation="MNKLFLLSLSAAVIVFAGCGSNHQPGAAAGDSAAKAPAAGTLLP
                     NPAAFRDTVDNQPTDLYILHSGKFAAAITNYGARLVSLIVPDKNGAATDVVLGYDSIG
                     KYIHQPDTYFGAIVGRYGNRIGKARFKLNGKEYRLYKNNGANTLHGGKKGFDAVVWTA
                     ERVDSSSVRLTYLSKDGEEGYPGNCKVTVTYSLDSNGLRIDYAATTDKPTVLNLTNHS
                     YFNLNGQGSGTINNHTLQINAANFTPVDSGLIPTGRIESVAGTPLDFRQPAAIGARVN
                     DSANKQIRFGRGYDHNFVLDAACAGGRAPAAIVTGDRSGIVLTVYTDQPGIQFYGGNF
                     LTGTNPLKAGKTDGYRTAFCLETQHFPDSPNEPSFPDTELKPGAEYHSTTRFVFSAK"
BASE COUNT         1115 a         1452 c         1317 g         1019 t
ORIGIN      
        1 tcggcgaaga cctgggaatg gtcccccgct gcgtcccgga tgtaatgcgt cgcctgggca
       61 tcctgagcat ggagatccag cgcatgccaa agaaccccaa ccgggaattc tttcatcctg
      121 ccgaagcacc ctatctgtct gtcgtgacgc catcgaccca cgacatgagc accatccgcg
      181 gctggtggga agaggaccgc atcaagaccc aacgattctt caataatgaa ctcggtcagt
      241 ggggcgaggc gccggtgttc tgcgagccgt ggatcaaccg cgccatcatc ctgcaacacc
      301 tgaattctcc cgcgcaatgg tgtgttttcc agctccagga cattatgggc atgagtgaag
      361 ccttccggcg cgagaatccg cacgacgaac ggatcaatgt tcctgccaac ccgcatcatt
      421 actggcggta ccggatgcat ctgacactcg aggaactgat ggacgagaag gagttcaata
      481 acgggttgcg ggaaagcata aaggccagcg gaagataatt cctgctggcc tcgctcggat
      541 cgcggcgaac cttccgcgat ctgtatcgaa acggttggta tcagatgact tttacaaagg
      601 gctgtacgaa gtggaacgag ccgctctggc tgctcacttt caacacgtag ttgccggccc
      661 gcaggttgtt gatcgaaacc gtttccaggt tgctgcctgc ctgcaactcc tgctggctgg
      721 aactgaccat gacgcccgtc aggctataaa tctgtaccag gacttcctgt gaagcgctgg
      781 ccgtgaccgt ataattgatg gtggccactg caggattcgg gtaaagtccc atcctatcgg
      841 cggcagattg accgttttcc agccggatga ccttggaaaa ccgctgaacg ccacccatat
      901 cgacttcgcg caaccggtaa caattggtac cggggagcat attttcgtca gaaaaagaat
      961 aattggtcgt atttccgttg ttgataccca taacaacagc gatcgaatca aaaccgccgt
     1021 cggctccggc gcgctcgacc acgaaccacc tgctgttggt ttcattctca gtcacccagg
     1081 ccagatcagc gcaggatcca tcggcggagc cggtaaaact gagcaaaagg gaaggggtaa
     1141 cattctgctc ggcaacttcc tggcagaaag cagtattggc caccagggca gcgagggcga
     1201 gcgtgaaaag ggtacgtttc gatttcattt ggttcggatt atgccagatt aggcatcaaa
     1261 aagatgccaa aagagaacag attgaatgct aactcattgt gtcggcggga ggtccaagag
     1321 ctttcccgga atcggaaaaa agttctcaaa aatgcaaaaa tctagtataa tcccgcctgt
     1381 gactccgtaa tttccgaagg gccgcttccc ggcggtttcc cttatttttg tccggaatga
     1441 ccctcaatta ttacagccat aacctcgcct ttcgacatcc tttcacgatc tcccgcggta
     1501 caaagaccca ccaacccacc ctgattgtcg aactgggctt gggaacgctt aagggctatg
     1561 gcgaagctcc tgccatcagt tattaccata tcccggtggc aagaatggtc gctgacctgg
     1621 aagccaaaag gcctttaatc gaaaagtttg ccttaaccga cccggaacgc tattggcatt
     1681 acctccatca cctgtttccc gacaacccat tcctggtgtg cgcccttgat atggctgcct
     1741 gggacctctt cggccgcctt catggccagc cgctgtataa gttatggggg ttgaacccgt
     1801 ctaaagcacc cctgaccgat tataccatcg gcatcgatac ggttgcgaaa atggtcggga
     1861 aaatgcagga aaaaccgtgg ccgatctata agatcaagct cggcaccccg gacgatatcg
     1921 cgatcgtcag cgagatccgc aaacataccc ggtcaaccct tcgggtcgac gcgaatgccg
     1981 gctggaacct tgcggaggcc ctggataaga tcccacgcct cgctgcgctc ggcgtcgagc
     2041 tcgtggaaca gcccctgccg agggacgacc ggcaaggcat gaaagcgttg ttcgccgcct
     2101 cctcccttcc gctgattgcc gacgagtcct gcgttcgtga ggaagacgtc gaaacctgcg
     2161 cgggccattt ccatggcatc aatatcaagc tgaccaaatg cagcggtatt actccggcga
     2221 gaagaatgat cgcggcggcg cggtcgcttg ggatgaaggt catggccgga agcatgaacg
     2281 aaagcagcgt cggcagcgcc gccattgccc accttctccc atatctcgat tacgtcgata
     2341 tggatggacc cttattgctt tccgaggatc tgggcaccgg actggagttt gattacggaa
     2401 aagttaccat tccacaggaa cccggcctgg gcgttacgta caccggcgtt ttttccaaac
     2461 cctgaccatg ccgtatcgtt tttagctgta gatttgacat ttatgtcccc atcctccctg
     2521 gtcagagaaa aatatctcgc agcctttggc ggcaacccgg tccttgtgcg gtctccaggc
     2581 cgtgtcaatc tcatcggcga acataccgat tacaaccacg gctttgtttt gcccgccgcg
     2641 atcgataagg ccatttacat ggcggcaggc cgccgggagg atcgcgagat ccatctcatg
     2701 gccctggacc tgcagaagat gtaccgcggc tcgctcgaca agctcgaggc ctcttcccag
     2761 cactggcccg actacctcct cggcgtggtc cagcagctgc tccaggcgga ccaggtccgg
     2821 gatgccggtc gcccgatcgg tggcttcaac atcgtcttta ccggcgatat tcccctcggc
     2881 gccgggatgt cgtcttcggc agcactcgag tgtgcgacgg catttgccct caacgaactc
     2941 tttggcctcg acctggaccg gatgacgatg gtgcgtcttg cacagaaagc cgaaaatcaa
     3001 tttgtggggg ttaaatgtgg gatcatggac cagtttgcca gcatgttcgg ccgccgtgac
     3061 cacgtgatcc gtctcgactg cgacagcctg gcctacacct attttccgct gaaccttaac
     3121 gatattcgca tcgtcctgct ggatacgcac gtccaccatt cgctggcgtc ttccgaatat
     3181 aacacccggc ggcgccagtg tgaagcgggc gtgaaactcg tccgggcctg ccatcccgaa
     3241 gtcgccagcc tgcgcgatgt cacgcttgac atgctggacg aatatgtggc gcccatcgac
     3301 gagttggttt acagacgctg ccggtttatt atcagggaaa acaaccggct gctggcaggc
     3361 acggaagacc tgcaaaaggg cgatctcgaa tcgtttggcc tgaaaatgtt cgaaacccac
     3421 gacggcctta gccgcgacta cgaggtgagt tgcaaggaac tggattttct cgtggcgggg
     3481 gtcaggaacg accctgcggt tctcggcgcc cgcatgatgg gcggcggatt tggcggctgc
     3541 acgatcaacc tggtaaaaaa aggggcgata gacaacctca tcgcgcggat cgggccggtg
     3601 taccgcaaag cgatgaacaa ggaactgact gcatatatcg caagaatcga agacggaaca
     3661 tctctcatgc agtcctaaca acccaggacc tgtcaaccat aattttcgct atatgaataa
     3721 actgtttcta ctatcgctaa gcgccgccgt cattgttttc gccggttgcg gttccaacca
     3781 tcaacctggc gcggccgcgg gcgacagcgc cgcaaaggct ccggccgccg gcacgcttct
     3841 gcccaatcct gctgcattcc gcgacaccgt ggacaaccag cccaccgatc tctacatcct
     3901 tcacagcggc aagttcgccg ccgcgattac caactatggt gcccgcctgg tgagccttat
     3961 cgtcccggac aagaatggcg ccgcaacgga tgtggtcctc gggtacgaca gcatcggcaa
     4021 gtacattcac cagccggaca cctacttcgg cgccatcgtc ggccgctacg gcaaccggat
     4081 cggtaaagca agattcaaac tgaacggtaa agaatacagg ctatacaaaa acaatggcgc
     4141 caacaccctg cacggcggta agaaaggttt tgatgccgtc gtgtggaccg ccgaaagggt
     4201 cgactcgtcc tcggtccggc tgacttacct ttcaaaggac ggcgaggaag gatatccggg
     4261 aaactgcaag gttaccgtca cctattcgct cgacagcaac gggcttcgga tcgactatgc
     4321 cgcgacgacc gacaaaccca ctgtcctgaa cctgaccaat cattcctatt tcaacctgaa
     4381 tggccaggga agcggcacca tcaataatca tacgctccag atcaatgcgg cgaactttac
     4441 gccggtcgat tccggcctga tccccacggg caggatcgag tcagtcgccg ggacgcccct
     4501 tgacttccgc cagccagcgg ccatcggggc gcgcgtgaat gacagcgcca acaaacagat
     4561 ccggtttggc cgcggctatg accacaattt tgtcctcgac gccgcctgcg cgggaggacg
     4621 tgctccggcg gccatcgtta ccggcgaccg gagcggcatc gtgctgaccg tctatacaga
     4681 ccaacccggc atccagttct atggcggcaa ttttcttacc ggaaccaacc cgctcaaggc
     4741 tggcaagacc gacgggtatc gcaccgcttt ctgtctggag acccaacact ttcctgattc
     4801 tccgaatgaa ccttcattcc cggacacgga gctgaagccc ggtgccgaat atcattcgac
     4861 aacaaggttt gtgttctcgg caaaatagcc gggcctaacg cgg
//
LOCUS       DATGKE010000465         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_654299, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000465 DATGKE010000000
VERSION     DATGKE010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654299"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..916
                     /locus_tag="VKU83_08020"
     CDS             281..916
                     /locus_tag="VKU83_08020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLI93541.1"
                     /translation="MSLRLGDKAPNFKAKTTIGEIDFYEYLGDGWGILFSHPADYTPV
                     CTTELGKTAALKDEFAKRGVKVLALSVDPVEKHLGWIGDINETQNVSVDFPIIGDEDR
                     KVSTLYEFIHPNASATATVRSLVVIGPDKTVKLIITYPASTGRNFQEVLRVVDSLQLT
                     ANHSVSTPADWKQGEDVIVANTISTEDAQKKFPKGVKVVKPYLRYTPQPTV"
     gene            complement(965..1438)
                     /locus_tag="VKU83_08025"
     CDS             complement(965..1438)
                     /locus_tag="VKU83_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93542.1"
                     /translation="MQPRDKIRLYFFLGLLATALLYSAYNLFILDPYVYERMSRGTRH
                     IYKFGAVIISWLIGFLVFRKRTAGWLVLIWHLAFGVGLAILILFAGYDAAIRELPPPV
                     RQPVSTFHEFLISPIPYVFIGIVSRAVRRNPESGPTPPPGPTSPPGPPASTKEKG"
     gene            complement(1422..2039)
                     /locus_tag="VKU83_08030"
     CDS             complement(1422..2039)
                     /locus_tag="VKU83_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HLI93543.1"
                     /translation="MIRSSGFRQIPKPVREFLLKATVLFVAWKAVYLLFLLPGRVLDK
                     PLTYVIGKGTAATLSVIHPSGNFNAIATMHPKGLGNEGLEPVMAIRSNQTVLLSIADV
                     CNGLELLMLYAGLILCLPASRSRKLGYIVAGIVLIEIINVIRCAGLVLVYLHKPAYLD
                     FSHHYLFTFVVYAFIFWLWYLFSRDPEFSSKLLHPNPAIENATPR"
     gene            complement(2787..3317)
                     /locus_tag="VKU83_08035"
     CDS             complement(2787..3317)
                     /locus_tag="VKU83_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93544.1"
                     /translation="MTGRLRHIAVSVWVLAAAASPWAARGQNPGNPQNPVTLTPAGGN
                     IPQTPETAKAIVAATGFMNAIVGDSSSGTLMNLCGLPFCHDDSVILTTRDQLRNALTQ
                     LITATSHERARTHPRVDTAYVLNIRKEALFGLVPINIYFTVVRLKFTVGGKDAFRLVI
                     LAVQLTDDARVVGIED"
BASE COUNT          890 a          862 c         1046 g          749 t
ORIGIN      
        1 cgacagccca acccgccctg tttgagcact ggaagcaggg cgttactccg cttgttcgac
       61 aatgaaaagc cgtatgacgg gagactgtca cgtacggttt tgtgagagct tagggctgaa
      121 atgctctatt ctactcgact agatgtggca ggttagcatg gccacgagga acttcacgcc
      181 gtgagtatca accagttgga atgatttacg aaaatttaga acaataagtc tactatatta
      241 atagggaatt gatatcttta cggtccaaat ttttaagcat atgagtctac gtctagggga
      301 caaagctccc aactttaaag caaagacaac gatcggagaa atcgattttt acgaatacct
      361 gggtgacggc tggggtatcc tcttctctca ccctgccgac tatacgccgg tgtgtacgac
      421 cgaactggga aagacggccg cgctcaagga tgagtttgca aaacgtggcg taaaggtcct
      481 tgcgctgagc gtcgacccgg tggaaaagca tctaggttgg atcggtgata tcaatgaaac
      541 gcagaacgtc agcgtggatt tcccgatcat cggggacgaa gacaggaagg tgagcacctt
      601 atacgagttc atccatccta atgccagcgc tacggctacg gttcgcagtc tggtcgtgat
      661 cgggcccgat aaaacggtca agctgatcat cacctatccg gcttccacgg gtcgcaattt
      721 ccaggaagtg ctgcgggtgg tggattccct gcaactgacc gccaaccaca gcgtatcgac
      781 tcccgccgac tggaagcagg gcgaggacgt gattgtggcg aataccattt ctaccgaaga
      841 tgcgcaaaag aaattcccga aaggggtaaa ggtcgtaaaa ccttacctga gatatacgcc
      901 gcaacccacg gtttagacgt ttgtaattga aaatggttcc ttatagggta agccgcagcc
      961 gggcttaccc tttttcttta gtagaagcag gcggcccggg cggcgaggta ggtccgggcg
     1021 ggggagttgg cccgctctcc gggttgcggc gaacggcgcg ggagacgatc ccaataaaaa
     1081 catatgggat gggcgagatc aggaactcat ggaacgtact gaccggctgg cgcacgggag
     1141 gcggaagttc ccggatggcg gcatcgtagc cggcgaacag gataaggatg gcgaggccaa
     1201 cgccgaaggc gaggtgccag atcaggacca gccagccggc ggttcgtttc cggaagacaa
     1261 ggaaccctat gagccaggag atgatcaccg cgccgaattt gtagatgtgc cgtgtgccgc
     1321 ggctcattcg ctcgtagacg tagggatcga ggataaagag gttgtaggcg gaatagagaa
     1381 gcgcggtggc cagcaggcca agaaagaaat aaagccggat cttatcgcgg ggttgcattt
     1441 tctatcgccg ggtttggatg gagcagcttg ctggagaatt cggggtcgcg cgagaaaaga
     1501 taccacagcc agaatatgaa agcatacacc acgaaggtaa aaagataatg gtgggaaaaa
     1561 tcgagatacg cgggcttgtg aagatagacc aggacgaggc cggcgcaccg aatgacgttg
     1621 atgatctcga tcaggacaat gcccgcgacg atgtaaccga gtttccggct gcgggatgcc
     1681 ggaaggcaca ggatcagtcc cgcgtagagc atcaggagtt cgaggccatt gcagacatcg
     1741 gcgatcgaga gcagcaccgt ctggttggat cgaatggcca tgacgggctc gaggccctca
     1801 ttgcccaggc ccttcgggtg catggtcgct atcgcgttaa aattgcccga aggatgaatg
     1861 accgataggg tagctgctgt cccttttccg atcacatacg taagcggttt atccaaaacc
     1921 ctgccaggca gcaggaataa tagataaacc gctttccaag ccacgaatag tacggtggct
     1981 ttcaatagaa attctcttac aggtttagga atctgcctga acccggatga tcggatcata
     2041 ccatttgctt ccttgcctta gcccttttta tgcccaggcc aatgcctgca gcaagcagta
     2101 ggctaatacc gccgtcaata ggggccgttc cgccgccgcc gcttgccggg tcccatgcgg
     2161 gatgtccggc gccaggaaag cggtcatgtc cggcaacgat cgaacgatca aaaaggtcgt
     2221 tcctgtcaat gtcagggtgc ggattgccga tcggatcctg cagatgtaac ggattgtcgt
     2281 cctgcaagga cgaacgccag ccgtttacat ctcgattgtc tgaattgagg ggatccgatt
     2341 gatgaatacc gaaaggatcg agcccaaggc gggacctttc gccgtagtta gtcctttggc
     2401 ccggaccggg gccgccagcg taagcccttg aaacgattag tagcgggaat acgaaagcgg
     2461 tcagaatgag taagcgcgtt acttttttca tgagagtaat aattattgat cattataagt
     2521 ctaactgccg agaacttgac ttttaaaagg ctttcgtaga atgttagcag gaaaatatgc
     2581 ggccacttaa ggagtatggg gggctcaaca cggcaactta ggagatagga ggtaagtgaa
     2641 tcaaaggtac gaataaaatc gatactgcaa tgcgatagta caaaaagcca gccaaagttt
     2701 tgcaccgtat ccatgcgcgc attcgggatc ggaaaactac gaggattcac ccatccgcgg
     2761 cgttccagct ggggaaatca cgccacttag tcctctatgc caaccaccct ggcgtcgtcg
     2821 gtgagctgca cggcgaggat aaccagccga aatgcgtctt tcccgccgac ggtgaactta
     2881 agtctgacaa cggtaaagta aatgttaatg ggaaccaggc cgaagagtgc ttccttccgg
     2941 atgttcagga cataggccgt atccacgcgg ggatgggtgc gggcgcgctc atgggaagtt
     3001 gcggtaatga gttgagtgag cgcattgcgc aactggtccc gggtcgtcag gataacgctg
     3061 tcgtcgtggc aaaaaggtaa cccgcacaga ttcatcaggg taccggagct gctgtcaccg
     3121 acaatggcgt tcatgaatcc ggttgccgcg acaattgctt ttgccgtctc tggcgtctgg
     3181 gggatattac ccccggcagg cgtaagggtg acggggttct gcggattacc gggattttgg
     3241 ccgcgggcag cccagggtga cgcggccgca gccagcaccc aaaccgaaac ggcaatatgg
     3301 cgaagacggc cggtcatgct gcaaatgtag gaaattgaag gagttaaggg ttcgctatta
     3361 ggggataaat ggcggtccgg ataatttact tattttcagc tacccaaaca cccgcttatg
     3421 aagagatcat tgctcctcgc cctggttctc gcccccacat tgctgattgc ccagcggacg
     3481 ctgctttact gcggcaagct gatcgacccg aaggcgggtc aggtgttgac cgggatgtcg
     3541 gttatcg
//
LOCUS       DATGKE010000466         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_872888, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000466 DATGKE010000000
VERSION     DATGKE010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872888"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VKU83_08040"
     CDS             complement(<1..321)
                     /locus_tag="VKU83_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93545.1"
                     /translation="MATTKERFKKKADEVAAEIKDLLKDHGNDKIGEVTLSQIYQGMR
                     GITGLVSETSLLDAQEGIRFRGFSIPELQQRLPKAPGGTEPLPEGLFHLMLLGELPTE
                     EDVHE"
     gene            complement(387..1484)
                     /locus_tag="VKU83_08045"
     CDS             complement(387..1484)
                     /locus_tag="VKU83_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HLI93546.1"
                     /translation="MFKLLDTYILKKFLGTFVFTMLLITVIAVVIDTSEKADDFVRSG
                     LSFWQLVTHYFIGFIPFIMSMIFPLMVFIAVIYFSSKMAGRSEFIAILAGGVRYNRML
                     RPYLIGGIFLSVIFWLASQYWVPRANEIRTDFQAVYVDRNSSYTPDPTRDVKDYYLRV
                     DPHTFVDFRRYDTSSKMGVSFFLQKLKGNTVYYTINAAAIAWAPKTKDWRLTSVIERT
                     INGPEEKLRRLDTLHINLNVKPEELRRDEYLKDKLTTPELHQFIHMEEVRGSEGLNTF
                     KVELYHRDATPFSVIIMAMIGAIIASRKIRGGSGLHLAVGIVMASTFVVMDKFAVTFS
                     TKGNLPPMLAAWLPNIIFSGVAWVFYLKTPK"
     gene            complement(1542..2681)
                     /gene="tgt"
                     /locus_tag="VKU83_08050"
     CDS             complement(1542..2681)
                     /gene="tgt"
                     /locus_tag="VKU83_08050"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLI93547.1"
                     /translation="MSKLEFQLEGQCPRTAARAGRITTDHGEVLTPVFMPVGTAGSVK
                     AVSQQQLVGDVRAQIILGNTYHLYLRPGQEVLQQAGGLHRFNGWQRPILTDSGGYQVF
                     SLSGTRKIREEGVLFQSHIDGSRHMFSPEKVMDIQRIIGGDIIMAFDECPPYPSDHAY
                     AESSMRLTHRWLDRCWDRFHATGDLYGYSQHLFPIVQGGVYPDLRRASCEYVAGKDAA
                     GNAIGGLSVGEPEEEMYDICAACCEVLPAGRPRYLMGVGTPWNLLECIGRGVDMFDCV
                     LPTRNGRNAMLFTSKGVINIDNKKWELDFSPIDDGIDCGMSQFYSKAYLRHLIKSKEI
                     LGLTIASVQNLAFYLHLVREARARIITGDFAGWKQEQLAILKTRL"
     gene            2789..3919
                     /locus_tag="VKU83_08055"
     CDS             2789..3919
                     /locus_tag="VKU83_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93548.1"
                     /translation="MTKLYVGFPWPELFFIVFCLITLVQLVYYGFFFRRLAYYVPPAK
                     EQSQQHPVSVIICARDEAARLVTHLPGTLVQTYPTTHEVIVVNHNSQDDTRFLLDEFK
                     KTFKGLHIVNLEHEAIGIPGKKYPLSMGIKEARHEIVLLTDADCIPASEFWMQKMQDG
                     YHDGIEVVLGYSPYKKGPGILNKLIRFDTFHTALQYLSYALAGQPYMGVGRNLSYKKA
                     VFLRNKGFSAINHLPGGDDDLFINKVATGSNTAIVIDPDTFMVSEPKKTFGEWFRQKA
                     RHYSTAKFYKPKHKFLLGLYAISYVLFYPLFIISLFFSWQLSLIAFGLRLICQGIIFH
                     KTMKRLGEEDLFPWWWLLDIWMLPYYLIFSSVIWKKPQANWK"
     gene            3889..>4136
                     /locus_tag="VKU83_08060"
     CDS             3889..>4136
                     /locus_tag="VKU83_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HLI93549.1"
                     /translation="MEKAPGELEIVLRFFDEFSTGEIRQLAALGDLYREWNAKINVIS
                     RKDIDSLYEKHVLHSLAIAAAFDFQPGMEVIDIGTGGG"
BASE COUNT          921 a         1228 c         1009 g          978 t
ORIGIN      
        1 ctcatgcacg tcttcctcgg tcggcagctc tcccagcagc atcagatgga acaatccctc
       61 gggcaaaggc tcggttccgc cgggcgcttt tggcaaccgc tgctgcagct cggggatcga
      121 aaaaccgcgg aaccggatcc cttcctgcgc atccagcagg gaagtttccg aaacaaggcc
      181 ggtgatgccc cgcattccct ggtagatctg cgacaaagtc acttccccga tcttgtcgtt
      241 cccgtgatcc ttcaggagat ccttgatctc ggctgccacc tcgtcggctt tcttcttgaa
      301 cctttctttt gtcgttgcca tgttacccgt gtttttcggt ccactaaatt agttcacgat
      361 caattgcagt gcaaatgaat tctctattat ttcggtgtct tcaaataaaa gacccacgcc
      421 acgccgctga agatgatatt gggcagccat gccgccagca tcgggggcaa attgcctttt
      481 gtcgaaaaag tgactgcgaa tttatccatt accacaaaag tggatgccat cacgatcccg
      541 accgccagat gcaacccgct cccacctctg atcttcctcg atgcaatgat cgctccgatc
      601 atcgccatga tgattaccga aaatggcgtg gcgtcccggt gatacaactc gactttaaag
      661 gtgttcaggc cctctgaacc ccgaacctct tccatgtgaa tgaactgatg cagctccggc
      721 gtagtcagct tgtcctttaa atattcatcc cgcctcaatt cttcgggttt cacgttcaga
      781 ttgatgtgca gcgtatccag ccgcctaagc ttctcctccg ggccgttgat cgtcctttcg
      841 attacggatg tcaacctcca gtccttcgtt ttgggcgccc acgcaatggc cgccgcattg
      901 atggtgtaat acaccgtatt ccccttcagc ttctggagga aaaagctgac gcccatcttt
      961 gaggaagtat cgtagcgccg gaaatcgacg aaagtgtgcg gatccacccg caggtaataa
     1021 tctttcacgt ctctcgtcgg atcgggcgta taggagctgt tccggtctac atataccgcc
     1081 tgaaaatccg ttcggatctc gttggccctc ggaacccaat actggctcgc cagccaaaag
     1141 atcaccgaca ggaaaatccc gccgatcagg tagggacgca gcatccggtt ataccgcacg
     1201 ccccccgcaa ggatagcgat gaattccgaa cgacccgcca ttttcgagct gaaataaatg
     1261 acggcgataa aaaccatcaa cggaaagatc atcgacatga tgaagggaat aaaccctata
     1321 aagtagtgtg tcaccagctg ccagaaactg agtcccgaac ggacaaagtc atcggccttc
     1381 tcgctcgtat cgatgaccac ggcaatcacc gtgatcagca gcatcgtgaa aacaaaggtc
     1441 cccaggaatt tctttaatat gtaagtatcg aggagtttga acattgtccg ttttccggcg
     1501 caaaaatagc gcttttcccg gatggatgag ttaacggttt cctatagtct ggtcttaagg
     1561 atcgccagtt gctcctgctt ccacccggca aaatccccgg tgattatacg cgcgcgtgcc
     1621 tcgcgcacga gatgcagata aaaggccagg ttttgcacgc ttgcgatcgt cagcccgagg
     1681 atctccttcg acttgatcag gtgccgcaga taggctttgc tgtaaaattg gctcatcccg
     1741 caatcgattc catcgtcgat cggcgaaaaa tccagctccc attttttgtt gtcgatattg
     1801 atcacccctt tcgaggtgaa cagcatggca ttccggccat tccgggtggg caagacacaa
     1861 tcgaacatat ccacgccccg tccgatgcat tccaggagat tccacggcgt ccccacgccc
     1921 atcaggtacc gcggcctgcc agccggaagc acctcacaac aggcggcaca gatgtcgtac
     1981 atctcttcct ccggctcacc gacgctcagt ccgccgatcg cgttgcccgc ggcatccttt
     2041 ccggcgacgt attcgcaact ggccctcctg aggtcaggat atacaccgcc ctgcacgatg
     2101 ggaaacaggt gctgcgaata gccatacagg tctccggtag catggaaacg gtcccagcac
     2161 cggtccagcc accggtgcgt caagcgcatg gagctttccg cgtaggcatg gtcactcgga
     2221 taaggtggac attcgtcaaa tgccataatg atgtcgccgc cgatgatccg ctggatatcc
     2281 atcacctttt cagggctgaa catatgccgg gagccatcga tatgcgactg gaacagcacc
     2341 ccctcctccc ggattttccg ggttcccgat aacgaaaaaa cctggtaacc gccgctgtcc
     2401 gtcaggatgg gccgctgcca gccattgaag cggtgcaggc ctccggcctg ctgcagtact
     2461 tcctggccgg gccttaaata caaatgatag gtattgccga ggataatctg cgcccgcaca
     2521 tccccaacca gctgctgctg cgatacagcc ttcacgctgc cggctgtgcc gaccggcata
     2581 aaaacgggcg taaggacttc cccatggtct gtcgtgatcc ttcccgcccg cgctgcggtt
     2641 cgcgggcatt gcccctccag ttgaaattcc agcttgctca atatgccgcg caatttagtg
     2701 tgcggcgggc atttttccgt attgccgcta aaattgtact ttcgccgtgg caggcctggc
     2761 cggtgccgct gccccccatt gatcgcttat gactaaactg tatgtcggct tcccctggcc
     2821 tgaactgttt tttattgttt tttgtctgat caccctggtt cagcttgtgt actatgggtt
     2881 cttcttccga aggctggcct attacgttcc tcctgcaaaa gagcagtcgc agcaacatcc
     2941 cgtcagcgtt atcatctgcg cgcgggatga ggcggcacgc ctcgtgaccc acctgccggg
     3001 cacgcttgtt caaacctacc cgaccaccca cgaagtgatc gtcgtcaacc acaacagcca
     3061 ggacgatacc cggtttcttc tcgatgaatt caaaaaaacg tttaaaggct tgcatatcgt
     3121 caatctggaa cacgaagcga tcggcattcc cggaaagaaa tacccgctca gcatgggtat
     3181 caaggaagcc cgccacgaga ttgtcctgct taccgacgcc gattgcatcc ctgccagcga
     3241 gttctggatg cagaagatgc aggacgggta tcacgacggc atcgaagtcg ttctgggcta
     3301 cagcccctat aagaaaggtc ccggaatact gaacaagctg atccgcttcg acaccttcca
     3361 tacggctctc cagtatcttt cctatgccct ggccggtcaa ccctatatgg gcgttggccg
     3421 caacctctcc tacaagaaag ccgttttcct tcgcaacaaa gggttctccg ccatcaatca
     3481 cctgccgggc ggcgacgatg acctctttat caacaaggtg gccaccggca gcaacaccgc
     3541 catcgttatc gaccccgata cattcatggt ctccgaaccg aaaaagacct tcggtgaatg
     3601 gttccgccaa aaggccaggc attacagcac cgcgaaattc tataagccga agcataagtt
     3661 cctgcttggc ctttatgcga tttcgtatgt gcttttttat ccgctgttca tcatcagcct
     3721 gttttttagc tggcaactgt ccctgatcgc gttcggcctc cgcctgatct gtcagggcat
     3781 catctttcat aagaccatga aacgtctcgg cgaagaagac ctgttcccgt ggtggtggct
     3841 gctggatatt tggatgctgc cctattacct gatcttttct tccgtaatat ggaaaaagcc
     3901 ccaggcgaac tggaaatagt gctccggttc ttcgatgaat tctcgaccgg tgagatccgc
     3961 cagctggcgg cgctgggtga cctgtaccgg gaatggaacg cgaagatcaa tgttatttcc
     4021 cgcaaagata tcgacagcct ctacgaaaaa catgtgctgc attcccttgc tattgcggcg
     4081 gctttcgact tccagccggg catggaagtg atcgatatcg ggaccggagg cggctt
//
LOCUS       DATGKE010000467         6646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1156330, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000467 DATGKE010000000
VERSION     DATGKE010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156330"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VKU83_08065"
     CDS             <1..657
                     /locus_tag="VKU83_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI93550.1"
                     /translation="SAFKAPTLYQLYSAYGDPGLQPERSTSYEAGVQFDNRVLNARAT
                     FFHRQTKNGIDYNFFTNRYYNYDAEQGNGVEWEGSVRLSAVWSLSANYTWMKMTEQTE
                     SHVSYKDTTYRYALRIPQHTVNLTLGARPVAGLYLSLSGHYESKRYDLGGYDANFNPL
                     PDVVLDPFLIINGYAEYRLLRSLKLFAEGRNLLNRKFYPIYGYNAIPAMFTAGATVDF
                     "
     gene            677..1297
                     /gene="cobC"
                     /locus_tag="VKU83_08070"
     CDS             677..1297
                     /gene="cobC"
                     /locus_tag="VKU83_08070"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HLI93551.1"
                     /translation="MDSIYLIRHTTPAVAKGMCYGQSDLDVTESFAAETAAIRGALSP
                     AITSVFSSPLKRCSLLAQELFPGRPLRLMDALMEIDCGRWEMRPWDELPPEEIEPWMA
                     DLVQVRIPGGESYEDLRLRVSRAWETIGAEKGPGDIAVIAHGGVIRSILAGITGTSLI
                     ESFKAFSLHYGCVIRVYGAAGDWKYEVLLNEAPPEREQHKPKSFYR"
     gene            1367..1438
                     /locus_tag="VKU83_08075"
     tRNA            1367..1438
                     /locus_tag="VKU83_08075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1400..1402,aa:Arg,seq:ccg)
     gene            complement(1442..1939)
                     /locus_tag="VKU83_08080"
     CDS             complement(1442..1939)
                     /locus_tag="VKU83_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLI93552.1"
                     /translation="MNRFFPLAIISIIGFFAFQCGSNTQPATQNKSSADTSASRHKND
                     TVIMMAGINSKGIGRFDSVKLTHPLDQQLIAGGQAIYQSKCFACHKLTSELLVGPGWY
                     GVTKRRTPEWIMNWVTNTKVMLDKDAAAQADMVVCLIRMPNQDLTDEQARQVLEFMRK
                     NDGEK"
     gene            complement(2021..2323)
                     /locus_tag="VKU83_08085"
     CDS             complement(2021..2323)
                     /locus_tag="VKU83_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLI93553.1"
                     /translation="MKNPVAGNSSALVEAKAMYAANCTPCHGQKGKGDGPAAGGLNPR
                     PADHTSEAVQNQSDGAIYWKLSEGRMPMPSYRKIFTDDQRWGLVSYIRTLAKVKKK"
     gene            complement(2659..3840)
                     /locus_tag="VKU83_08090"
     CDS             complement(2659..3840)
                     /locus_tag="VKU83_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLI93554.1"
                     /translation="MIRRSIWAAAASLFLLALAACHSAKKGAFTVSGTYENADKLTAV
                     AGPVSKVFLLEISFSNQPPVLLDSARIPAGRGHFTLTTTATRQGIYELAFGDNILDVP
                     LINDAPEVSVNVDLGKKDDFYEVSGSDASKQLKDLITVFGQKNFEAERRAAAVDSLRR
                     ANAPDSTQQAAMTMGNLAIEDLSTYLRQFINSTSNPTVAALALTWGSHSFSKSDFETM
                     LNDLAKKYPDNAAIKDLKQRYDQEAAQLAQEQSEEADWKGKQAPDLSLPDMNGHPVSI
                     SSFRGKYLLVDFWASWCMPCRAENPNVVRAHNEFKGKNFAILGVSLDKDKADWQKAVQ
                     QDGLDWTQVSDLKYWNSKAVETFKFEGIPFNVLIDPQGKVIATALRGPDLENELKDVL
                     K"
     gene            complement(3837..5282)
                     /gene="gatB"
                     /locus_tag="VKU83_08095"
     CDS             complement(3837..5282)
                     /gene="gatB"
                     /locus_tag="VKU83_08095"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLI93555.1"
                     /translation="MHDAYEIVVGLEVHAQLLTRTKLFCGDDAGFGAEPNTQISPVTL
                     GYPGSLPRINRKAVEYAIRMGLACGSEIERTNYFARKNYFYPDLPKGYQISQHTTPVC
                     KGGKVAIRTAAGQREIRLNRIHLEEDAGKSIHDIDPENTLVDLNRAGVPLIEIVTEPD
                     IRSGEEAHAYLTELRKLLRYLGICDGNMEEGSMRCDANVSVRLTGVATLGTKVEVKNL
                     NSIRNVRRAIESEAERLIALSKAGGPILQETRSFDAATGKTFPMRAKEEANDYRYFAD
                     PDLPPFLVTDDFLGQVRDALPELPEALIARFQSQLQLPEYDARVICDDKDSAAYFERL
                     AVITSNHKAAANWLIGPVRSALHGKGLGWGDFPVTPAALARLIQAVEDGKFSFSMAAE
                     RIFPVLMEQPGRDPLEIAASLNLIQTSDIAEIESWVDQALAGMPDKVSEYKKGKRGLI
                     GLFVGEVKKISKGKADPKVTNRLLMEKLEIK"
     gene            complement(5425..6462)
                     /locus_tag="VKU83_08100"
     CDS             complement(5425..6462)
                     /locus_tag="VKU83_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLI93556.1"
                     /translation="MKSYLPKVAGLALLAMMIRTSAFSQDDNSEDSNHGSRHHVDEII
                     IRHKGDKDTKVTIEMKDGDVLVNGKPLSEFDDSSLSIRKMRINRYGNNSFSFNGDEFR
                     MPDIAEVPGTVRVFPNGPDGMGWNFNNGDLLGPRAFLGVATEKNNDGAGVKIVSINRE
                     KIGLKVGDIITRVDGEEITDPASLSEKIRSHQPGDKVSVTIVRDGKEQTQTVVLGKTK
                     TITGEPFNTYRFDMPDMKIAPRAGWPKEFPQIVSDQPRFGIRAQDAEDGKGVKVLDVD
                     DESTAEKAGIREGDVITRFDGKEVNSVAQLSEAAHAAKDKINVKVTVIRDGKSEELEV
                     RVPRKLHTADL"
BASE COUNT         1328 a         1895 c         1834 g         1589 t
ORIGIN      
        1 agcgccttca aggcgcccac cctctaccag ttgtactcgg catatggaga tccgggcctc
       61 cagcccgaaa ggtcgaccag ttacgaagcc ggggtccagt tcgataacag ggttctgaac
      121 gcccgcgcca ctttcttcca ccgccagaca aaaaacggca tcgattataa ttttttcacc
      181 aaccgctact acaactatga cgccgagcag ggaaacgggg tggaatggga gggatcggtc
      241 aggttatccg cggtctggtc cctgtccgcc aactatacct ggatgaagat gacggagcag
      301 acggagagcc acgtttcgta taaagacacg acctaccgct acgctttgcg gatcccgcaa
      361 cataccgtaa atcttacgct gggagcaagg ccggtcgccg gcctttacct cagcctgagc
      421 ggccattatg agagcaagcg gtacgatctc ggcggctatg acgccaactt caacccgctt
      481 cctgacgtag tcctggatcc ctttctgatc ataaacggtt acgccgaata ccggctgctc
      541 cgttccctga aattgtttgc cgaaggccgg aacctgctca acaggaagtt ttatcccatc
      601 tatgggtaca acgccatccc ggcaatgttt acggccgggg ccacggtcga tttctgaaac
      661 ggggtaactt tgatccatgg actcgatcta tcttatccgt cataccactc ctgctgtcgc
      721 caaaggcatg tgctacggac agagcgatct cgacgtcacg gagagcttcg cggccgagac
      781 cgcggcgatt cggggcgcat tgtcgccggc tattacgtcg gttttcagca gcccgctgaa
      841 acggtgcagc ctgctcgcgc aggaactctt tcccggccgg ccgctccggt taatggatgc
      901 gctcatggag atcgattgcg gccgctggga gatgcgccca tgggatgaac tgccgccgga
      961 ggaaatagaa ccatggatgg ccgatctcgt gcaggtgcgc attcccggtg gcgaaagtta
     1021 tgaggacctc cggctgcgcg tcagccgcgc ctgggagacg atcggggctg aaaagggacc
     1081 gggggacatc gcggtgatcg cacatggcgg ggtgatccgg agcatcctcg ccgggatcac
     1141 cggcacgtcg ctgatcgaaa gttttaaagc tttctcgctg cattacggtt gcgtgatccg
     1201 cgtgtacggc gccgccggcg actggaagta cgaggtcctt ttgaacgagg cgccgccgga
     1261 acgtgagcag cacaagccga aatcatttta ccggtaattc gatttaatta agtagttttg
     1321 ccgtcccttt tccggccggc cagccctccg ggcagggtga atttctggtc ctgtagtaca
     1381 acggatagta taagggtctc cgaagccctg gattccagtt cgattctgga cgggaccaca
     1441 ctcatttttc gccgtcattc tttcgcatga attccaacac ctgccgcgct tgttcatccg
     1501 taaggtcctg attgggcatt ctgatgagac aaacgaccat atccgcctgg gcagcggcat
     1561 ccttatccag cattactttg gtattggtaa cccagttcat gatccattcg ggggttcgcc
     1621 ttttggttac gccgtaccag ccggggccaa ccagcaattc gctggtgagt ttatgacatg
     1681 caaaacattt tgattggtag atcgcctgcc cgcccgcgat cagctgctga tccaatgggt
     1741 gggtaagttt cacgctgtcg aaccttccga ttcctttcga attgatgcct gccatcatga
     1801 tcaccgtgtc atttttatgc cgggaagcgc tggtatcggc tgaagattta ttttgcgtag
     1861 cgggctgggt gttggacccg cattgaaacg cgaaaaaacc aataatagag attattgcta
     1921 agggaaaaaa gcgattcata catttcggtt ttattgtcag atcaagatga aattccgggc
     1981 aaggtcgcac tcgacaagtc ctggcgaaaa agcaatcggc tcatttcttt ttgactttgg
     2041 caagcgtgcg gatataagac accaaacccc acctttggtc gtcggtaaag atcttccggt
     2101 aggagggcat gggcatcctg ccttcagaaa gcttccagta aatggcgccg tcactttggt
     2161 tctgcaccgc ctctgacgtg tgatccgcag gccgggggtt taatcccccc gcagccgggc
     2221 catccccttt gcccttttgg ccgtgacagg gggtgcagtt agcggcatac attgcctttg
     2281 cctccacaag cgcagagctg tttcccgcaa cggggttttt caaattatcc gcagatgacg
     2341 gggcaaccca gggcttattc tgtgcaatgg ctccgtcaaa aatcaatatt aggatgataa
     2401 ggcccgcaac cagcgacctg tttttaaacc ctgaataaat ccttggtgaa gattttttca
     2461 tcataataaa taaacttttg attaatgggc gaatatttaa tcgatgggca agtttttcaa
     2521 tgaatgggca agtgaaatta tcctccggcc ggggtagtgg gactgacttt cgtcagccgc
     2581 cggcatgact ttggtagaaa aaaaaccgcc cagggcgggc ggcagcggcg aaaattcccg
     2641 aaaggcctat aaaacgttct atttcagcac atccttcaat tcattttcca ggtcaggacc
     2701 gcgaagcgcg gtcgcgatca ccttcccctg cgggtcgatc aggacattaa aaggaatacc
     2761 ttcgaatttg aaggtctcca ccgccttgct gttccagtac ttgagatcgc taacctgtgt
     2821 ccagtcgagc ccgtcttgct ggacggcctt ttgccagtcg gccttatcct tgtcgagcga
     2881 gacgccgagg atcgcaaagt tcttgccctt gaactcgttg tgcgctctca cgacattggg
     2941 gttttccgcc cggcagggca tgcaccagct tgcccagaag tccacgagca agtacttacc
     3001 acggaaagaa gagatcgaga cgggatggcc gttcatatcc ggcagcgaca ggtccggcgc
     3061 ctgcttgcct ttccagtccg cttcctcact ttgctcctgc gccagttgcg cggcttcctg
     3121 gtcataccgc tgtttcaggt ccttgatggc cgcgttatcc ggatatttct ttgcgaggtc
     3181 gttcagcatc gtctcaaaat cgcttttgga aaaactgtgg ctgccccagg tcagcgccag
     3241 ggcggcaacg gtgggattcg aagtgctgtt gataaattgc cggaggtagg tgctgagatc
     3301 ctcgatcgcc agattgccca ttgtcatcgc agcttgttgg gtgctgtcgg gtgcattagc
     3361 ccggcgaagg ctgtccaccg cagccgcccg ccgttccgcc tcgaaattct tctgaccaaa
     3421 aaccgtgatc agatccttca gttgcttgct ggcgtccgag ccgctcacct cgtaaaagtc
     3481 atctttcttc ccgaggtcca cattcaccga aacctccggc gcgtcgttga tcagcggaac
     3541 gtcgaggata ttgtccccaa aggccagctc gtaaatgcct tgcctggtgg cggtcgtcgt
     3601 gagcgtgaaa tggccccggc cggccgggat gcgtgccgaa tccagcagga ccggcggctg
     3661 gttggagaag gagatctcca acaggaacac cttgctgacg ggacccgcga cggcggtcag
     3721 tttatcggca ttctcataag tcccggacac ggtaaaagcg cccttctttg cggaatgaca
     3781 ggctgccagc gccagcagga aaaggctggc ggcagccgcc caaatggatc gtcttatcat
     3841 ttaatttcca gtttttccat taataatcgg ttggtcactt tcggatcggc cttgcctttc
     3901 gagatctttt ttacctcgcc gacgaaaagg ccgatcaggc ctctcttgcc ttttttgtat
     3961 tccgaaactt tgtccggcat tcccgccagc gcctggtcga cccagctttc tatctcggcg
     4021 atgtcggagg tttggatcag gttaagcgag gccgctatct cgagcggatc cctgcccggt
     4081 tgttccatca ggaccgggaa aatgcgctca gccgccatcg agaagctgaa cttaccgtct
     4141 tccacggcct ggatcaggcg cgcgagcgcg gcgggcgtaa ccgggaaatc cccccagccc
     4201 aggcctttcc cgtgcagcgc ggacctgacg ggaccgatca gccaattggc ggcggcttta
     4261 tgattgctgg tgatcaccgc cagccgttca aaataggcgg cggagtcctt atcgtcgcag
     4321 atcacgcgcg catcgtattc aggcagctgc agctggcttt ggaaccgcgc gatcaaggcc
     4381 tcgggaagtt ccggaagggc gtcgcggacc tggccgagga agtcatcggt tacgaggaac
     4441 ggcggcaggt cggggtcggc aaaatagcgg tagtcattcg cctcttcttt ggcgcgcatc
     4501 gggaatgtct tccccgtggc agcgtcgaag ctgcgggtct cctggaggat ggggccccct
     4561 gctttggaca gggcgatgag ccgttccgct tcggactcta ttgcccggcg tacattgcgg
     4621 atggagttga ggttcttcac ctcgaccttt gtccccagcg tcgcgacgcc ggtaagccgc
     4681 acggacacgt tcgcgtcgca gcgcatcgac ccctcttcca tatttccgtc gcagatcccg
     4741 aggtagcgga gcagcttccg gagctccgta aggtaggcat gggcctcttc gccgctacgg
     4801 atgtccggct ccgttacaat ctcgataagg ggtacgccgg cgcggttgag gtcgaccagc
     4861 gtattttccg ggtcgatatc gtgaatgctt ttcccggcat cctcttcgag gtggatgcgg
     4921 ttaagccgga tctcccgctg gccggcggct gtccggatgg caaccttgcc ccccttgcag
     4981 accggggtcg tgtgctggct gatctggtag cccttcggca ggtccgggta gaaataattc
     5041 ttgcgggcga aatagttcgt cctctcgatc tccgagccgc aggccaaccc catccggatc
     5101 gcgtattcca cggctttccg gttgatccgg ggcagggaac cgggatatcc cagcgtcacc
     5161 gggctgattt gcgtattcgg ctcggctcca aagcccgcgt catcgccgca aaacaacttc
     5221 gtacgggtca gtaactgggc atgaacctcg agtcccacca cgatctcgta tgcatcatgc
     5281 atactgcaaa tttattgatt tatgcgccaa caggcattat ttatgatcag cggggcgggt
     5341 tgaagctgcc gcctgaatga gacaggcagg ccgcgtggcc tgcctgtagg gaatcctatc
     5401 tgctgatgga aaagcccccg ctgatcacag atcggccgta tgcagtttcc tggggaccct
     5461 gacctccagt tcctcacttt tgccgtcgcg aatgacggtc acttttacat tgatcttgtc
     5521 ttttgccgcg tgcgccgcct cgctcaactg ggcaaccgaa ttgacttcct taccgtcaaa
     5581 ccgcgtgatg acgtcccctt ccctgatccc tgccttttcc gcggtcgatt cgtcgtcgac
     5641 atccaatacc tttactcctt tgccgtcttc agcgtcctgg gcccggatgc cgaaccgcgg
     5701 ctgatcgctt acgatctgag ggaattcctt tggccagccc gcccggggcg cgattttcat
     5761 gtccggcatg tcgaacctgt aggtgttgaa cggctcgccg gtaatcgtct ttgttttgcc
     5821 gagcaccacc gtttgcgtct gctccttgcc atcccggaca atggtcaccg ataccttgtc
     5881 gccgggctgg tgcgaccgga tcttttccga aagcgaggcg gggtcggtaa tctcttctcc
     5941 gtcaaccctg gtaatgatat cgcccacctt cagcccgatc ttttcccggt tgatggaaac
     6001 gatcttcacg cctgcgccgt cgttattttt ttcggtcgcg acccccagga aggccctcgg
     6061 gcccaataga tcgccgttgt tgaaattcca gcccatgcca tccggaccgt tcgggaagac
     6121 ccgcaccgtt cccggcacct cggcgatatc cggcatccga aactcgtccc cgttaaagct
     6181 gaagctgttg tttccgtacc ggttgatccg cattttgcgg atggacaggc tgctatcgtc
     6241 gaactcggac aggggtttgc cgttcacaag aacgtcgccg tccttcattt cgatcgtgac
     6301 tttcgtgtct ttgtcgcctt tgtggcggat gatgatctca tcgacatgat gccgtgaacc
     6361 gtggttggag tcctccgaat tgtcatcctg cgagaaagct gacgtccgga tcatcatggc
     6421 gaggagggcg aggccggcga cctttgggag ataacttttc atcagtggtg atttttgata
     6481 aactaaaggt ttttgatcta cgttgatttt cgtagatcaa atataagtgt attttcccaa
     6541 atacgaaaac cttcggaaat gttaaatcgg ggcaacgata tacatagttc cggcgggtca
     6601 cccgagcagt gccgccacct tttctatcac ctgggaaaga tttccg
//
LOCUS       DATGKE010000468         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_906692, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000468 DATGKE010000000
VERSION     DATGKE010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906692"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1007..1080)
                     /locus_tag="VKU83_08105"
     tRNA            complement(1007..1080)
                     /locus_tag="VKU83_08105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1044..1046),aa:Met,seq:cat)
     gene            complement(1583..>2655)
                     /locus_tag="VKU83_08110"
     CDS             complement(1583..>2655)
                     /locus_tag="VKU83_08110"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLI93557.1"
                     /translation="DFKVLEYSHSAGNESYRRKLADYYRRVGIAVDYSQILITTGGSE
                     AILFGFMSCLDPGDEVIIPEPFYANYNGFAVEAGVVVKPLTSYISTGFALPPVAEFEK
                     LIGPRTRAIVICNPNNPTGYLYSREEMETLKELILRHDLYLFADEAYRDFCYSGTHTS
                     AMNLEGAEQNVLMMDTISKRYSACGGRIGALVTRNSRVLDTALKFAQARLSPPSFAQI
                     LGEAATGLPADYFDATRAEYLKRRDTVVGRLNRMAGVFCPNPGGAFYCMASLPVDDAD
                     RFCQWLLETFSADNQTVMLAPAAGFYGTPGLGKKEVRIAYVLNSDHLTKAMDCLEKAL
                     QQYPGRSPDHSMRKMAPDSYSK"
BASE COUNT          700 a          638 c          677 g          640 t
ORIGIN      
        1 atgcaacaac ataaggatat tcctgatctt cttgacttgc actgcaggca gcctcgatgc
       61 atcatttttt gtccaaagga gttttagccc cttatgttga atgcttgcga tcataggcaa
      121 atgtacccta ttacgttaca agttacaact attttgttat tgcttagata gaaattctta
      181 ggaaaggtcg taatctcctg gtattttcct aagttagcta ctccctcggc aagcggcgac
      241 aacgaaaggg tcgctcaccc gcccaaatag cgcccatttc gcattcgcgc ttttatttgc
      301 tatttttggt ttcttctaat aagacgaatg aaaccaattc aactcattgc cttattgctt
      361 ataacgtcct gtgccgctgc ccaatctccg gcaaccactt ctcccaggtt ccctaaagcc
      421 tttgacagta tgcgactttt aaatgccggg cccagccagg gagcgagcgt tgatacaatt
      481 aaggggttga atggggcgcg aatgctcacg gcgccacaat tctcggacac aagtttaacg
      541 gagaacgaga aagtggccgt ctgtatcaca gttgatagta cgggaaatgt cataagtgcc
      601 acttatcaaa gtcaaggaag tacaacaagt cgggatgagt acgtaaaaaa ggcgttggaa
      661 cgtgcttatc gggtgaaatt ttcgcccggc aattcggcat ccaagggtac gctcattttc
      721 gattttcaca ttcattaata catcattgaa cggctaagca agctgattaa agaaaaaggc
      781 aaaaagacac cttaccggta gcgaatgctt ttggtgcagc tgatatcagg acatgtggtg
      841 ttgcgggagg gttgcaaagc gaccaacgta aaaagagaga agctgcaatt cccaaaaatg
      901 taaataacag accgacgatc aatatagcgg cgtttctcga tggtctatga ggcacataaa
      961 aaataatccc ataataattt gaatatcaaa atactatggg attttgtagc gagatcggga
     1021 gttgaacccg agacctccgg gttatgaatc cgacgctcta accatctgag ctatctcgcc
     1081 ggctgtttca attcttttga aacgggtggc aaagatagag aaatgaccgg gaaaaaccag
     1141 aaattcggca ttgtccatcg cgggtccagg cacagtcccc gtgtccagca aaagatccag
     1201 gaggacggct ggctttgcaa aataaaaagg ccctctgtgg aaacagaagg ccgtctaacg
     1261 attgcttgcc atatgaaaat tcttgaaaga gcgattttgt ctgaaagccg aacaaagata
     1321 taccccgaat ccccaaccga ataataccaa atggttaaaa aactgccaat tgctaaatcg
     1381 gtttatcgcg ccggctcaag ttcttcgacc tgaacgcaag atcggttgtt ttctcgaaat
     1441 atgcaaagcc attttcaaac attttgggtc attttagaaa aaaaactcac atttcattta
     1501 tcataatata acaacgccgc ggccgtcagc cttgaaatgg gccaaatttg gcgaaattgg
     1561 cgaggaggca tttaaatagt aattatttac tatatgaatc tggcgccatt ttccgcatcg
     1621 agtggtcggg cgacctgccc ggatactgct gcaatgcttt ttcgagacag tccatagcct
     1681 tggtgagatg gtccgaattc agcacgtaag caattcggac ctctttcttt cccaggccgg
     1741 gtgtgccgta gaagccggca gcgggcgcaa gcatgacggt ttgattgtcg gccgaaaaag
     1801 tttcgagcag ccattggcag aaccggtcgg cgtcatcgac cgggagactg gccatgcaat
     1861 aaaacgcgcc gccgggattg gggcaaaaga cgcccgccat ccggttgaga cggccgacaa
     1921 cggtgtcgcg acgtttgagg tattcggcac gggtggcgtc gaaataatcg gccggaagcc
     1981 cggttgctgc ttcacccagg atttgcgcaa aggatggcgg actcagcctg gcctgggcga
     2041 atttcagcgc agtgtcgagc acgcgggaat tgcgggttac cagggcgccg atccggccgc
     2101 cgcaggcgct gtaccgtttg gaaatcgtat ccatcatcag gacgttctgc tccgcccctt
     2161 ccagattcat cgcgctcgta tgggtaccgc tataacaaaa atcccggtag gcttcatcgg
     2221 cgaacaagta caggtcatgg cgaaggatga gctctttcag ggtctccatt tcttcccggc
     2281 tgtagagata gccggtcgga ttgttcggat tgcagatcac gatggcccgc gtccgggggc
     2341 cgatcagttt ttcaaactca gccacgggcg gtagcgcaaa tccggtcgag atataggagg
     2401 tcagtggttt gacgaccacg cctgcctcga ccgcaaatcc gttatagttg gcgtagaaag
     2461 gctcagggat gatgacttca tctccgggat caaggcagct catgaagcca aagaggatcg
     2521 cttccgaccc gccggtggtg atgaggatct gcgaatagtc caccgcgatc ccgactcggc
     2581 ggtagtagtc ggccagcttc cggcggtagc tttcgttacc cgcgctgtgg ctgtattcga
     2641 gaactttgaa atcaa
//
LOCUS       DATGKE010000469         5330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_855680, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000469 DATGKE010000000
VERSION     DATGKE010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855680"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VKU83_08115"
     CDS             complement(<1..244)
                     /locus_tag="VKU83_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HLI93558.1"
                     /translation="MNRVVVTGMGAVTPLGNDPAAFWTGLTTGRSGAAPITHFDPVLF
                     KTRFACEVKNFDPGALLDRNELKRTDLFTQYALVAAD"
     gene            394..1437
                     /locus_tag="VKU83_08120"
     CDS             394..1437
                     /locus_tag="VKU83_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HLI93559.1"
                     /translation="MTDLSQFDANGPSNPRNNVFGLPFKEEDARLVILPVPWEVTVSY
                     GAGTARAAEHVFHASMQVDLFDAETGDAWRKGIYMRQADKKILMKSDYLRKEAELYIN
                     YTSKGEMVEKNTFMCKSLKEINEGSGYLNRWVYDQTRNLLNEGKLVGLLGGDHSTPLG
                     YIKAIAEKHGEFGILQIDAHCDLRQSYENFVYSHASVMYNALKETGELKKLVQAGVRD
                     YCQEEWDYIHNSGSRVITYFDKDIRERMFDGQTWKQIAEEIVSKLPEKVYVSFDIDGL
                     DPRLCLYTGTPVPGGFDTEQVFYLLRTMVRSGRKFIGFDLDEIGVGDTEWDANVGARV
                     LWKLCNLLIASNS"
     gene            1440..2003
                     /locus_tag="VKU83_08125"
     CDS             1440..2003
                     /locus_tag="VKU83_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93560.1"
                     /translation="MRYRYVVILKKEGERATDLLSVLLCFVSAVIFLLSQLKAAHPNY
                     YLYVFSVLLAAGLAINVVARGRGKRQVRFKYLLLLAALGWFGMPVFPWIGVLFLALAF
                     LEHQTKRPLEIGFDHEQVVINTLVRQRHAWSAFNNVLLKDGLLTLDFKNNRLLQKEVV
                     EDEDEENADEEEFNAYCRERLTAAYGK"
     gene            2126..3418
                     /locus_tag="VKU83_08130"
     CDS             2126..3418
                     /locus_tag="VKU83_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLI93561.1"
                     /translation="MPTSPFILYSDGKGNIFEDRSLYATGRSGWDAIPVPAEDWIGLP
                     DGGSLYELPGRKGIGLDVRTGEMRLCEKGWAVAAFVPPAHTGLYLAAYETGENAPTLP
                     LFCYTAAGWNDGKFLVPAVRIEQDIRQDCVGYDWDVIHSGVDRLLKAYPQNRLVRHLA
                     QNCCLTYHCPAARNYFMGRWECPVPSSPACNANCIGCISFQPQEETIPSTQDRLTFRP
                     TAEEIIEFTVPHLQTARYPIVSFGQGCEGEPLLMWETLAEAIRGMRRHTGRGSININT
                     NGSRPDAVRALCEAGLDSIRVSLNSARPSIYTAYYRPNNYRFADIIESLRIVRSYGGW
                     ASINYFVFPGMTDSTEEYEALRTLIRETDLTMIQWRNFNIDPDWYLGKIGVTETGEFL
                     GVRQLMELIREEFPAVRFGYFNPPMERIRGRYEADFAH"
     gene            3424..4134
                     /locus_tag="VKU83_08135"
     CDS             3424..4134
                     /locus_tag="VKU83_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI93562.1"
                     /translation="MSKVIIIGATSGIGRELARRYAAGGHLVGATGRRQELLATLAAE
                     YPGRVVTACYDATGRDAISESVSLAERLGGMDLLIYNAGWGEFSAGLDWDIDKQTVDI
                     NVNGFLMAVHFGWDHFIRQGHGHLVTISSVAGNRGNRYTPAYSAAKSFQSVYFEGLYI
                     KARRLNIPLYVTDIQPGFVNTQLAKGPRFWVASVDQAADQIVRAIARRRRKAYITRRW
                     WIVAKLFRWIPGWVYYRL"
     gene            4203..4391
                     /locus_tag="VKU83_08140"
     CDS             4203..4391
                     /locus_tag="VKU83_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93563.1"
                     /translation="MKRNQLIAISALSVVLAATTVYLIRKSRQNQRKRVVANAGYEMA
                     YDIHYPMKYSTRRRPAAG"
     gene            4479..>5330
                     /locus_tag="VKU83_08145"
     CDS             4479..>5330
                     /locus_tag="VKU83_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93564.1"
                     /translation="MSTYLPDSFERQKNLKASGYTATIVAALILLLYISWTVPTEVPP
                     VQEEGIEVNLGNSDKGMGVDQPYLPGKPAAENKEKYTPPKQAVVERQPAKDVETDDNQ
                     KDDAPVIKKPAVTRPDATKLPDKSVAKKVSRPVKQAEPEPVKPVVRHAKALMGTISGT
                     GNGGNDADDFKPGGNQGIAGGRGDQGAPGGDPNSNNYTGGGHGNGIAISRGLEGRHIL
                     TAPSFTGDFNYNAKVAVDVHVDENGNVTSAEYQMRGSTTSADNYVTTAKQKARLVKFN
                     PGSGESVG"
BASE COUNT         1231 a         1524 c         1482 g         1093 t
ORIGIN      
        1 ggtctgccgc cacaagcgca tattgcgtga aaaggtccgt tcgcttcaac tcgttccggt
       61 caagcagggc accgggatcg aagtttttga cctcacaagc gaaacgggtc ttaaaaagta
      121 ccggatcaaa atgggtgatg ggcgccgcac cgctgcggcc ggtggtcaaa cccgtccaaa
      181 aggccgccgg gtcgttcccg agcggcgtaa ccgcgcccat tcctgtcacg actaccctgt
      241 tcatattcaa aaatacttaa ttaccagtac gtaaggaaat taaaaggctg acctgcccgt
      301 tcctattttg ctatcttttc gaaccggcct aactttgcgt ccccaaaacc cggagaaaaa
      361 gtcttcgaag tcaccgcaat ttcgcacctc attatgacag acctatcaca gttcgacgcc
      421 aatggcccca gcaaccccag gaacaacgtt ttcggattac ccttcaaaga agaggacgcc
      481 aggctggtta ttttgccggt cccatgggaa gtgaccgtca gttatggagc aggaactgcc
      541 agggcggccg agcatgtctt ccacgccagc atgcaggttg atctttttga cgcagagacc
      601 ggcgatgcct ggcgaaaagg catctatatg cggcaggccg acaaaaagat cctgatgaag
      661 agcgactatc tacgcaagga agcggaattg tatatcaact acacctccaa gggcgaaatg
      721 gtggagaaga atacgttcat gtgcaagtcc ctgaaggaaa ttaacgaagg cagcggctat
      781 ctgaaccggt gggtttatga tcagacccgt aacctgctga acgaaggcaa actggtcggc
      841 ttgctcggcg gcgaccacag tacgccgctc gggtatataa aggctatcgc tgaaaagcac
      901 ggcgaattcg ggatccttca gatcgatgct cattgcgatc tccggcaatc atatgaaaac
      961 ttcgtctata gccacgcaag cgtcatgtac aatgccctga aggaaacggg tgagctcaaa
     1021 aagcttgtcc aggccggagt ccgcgactat tgccaggaag aatgggacta tatccacaac
     1081 agcggatccc gcgtgatcac ctatttcgac aaggatatcc gcgaaaggat gttcgacggt
     1141 cagacctgga aacagatagc cgaagaaatc gtcagcaagc tgccggaaaa ggtctacgtc
     1201 agcttcgata tcgatgggct ggacccgcgg ttatgcctgt ataccggcac ccctgttccc
     1261 ggggggttcg ataccgagca ggtcttttat ctcctccgga ctatggtccg cagcggcaga
     1321 aagttcatcg gcttcgacct ggacgagatt ggcgttgggg atacggaatg ggacgccaat
     1381 gtcggcgccc gggtcctctg gaaactttgt aatttgctga tagcaagtaa ctcctgacca
     1441 tgcgctaccg gtatgttgtc atcctgaaaa aagaagggga gcgcgcgacc gacctgttga
     1501 gcgtcctgct ttgtttcgtt tcggcggtca tcttcctgct cagccagttg aaggcggccc
     1561 atccgaatta ctatctctac gtcttttccg tactgcttgc agccggcctc gccataaatg
     1621 tcgttgcccg gggccgcggg aaacgccagg tccgcttcaa atatcttctc ctgcttgcgg
     1681 ctttgggttg gtttggaatg cccgtttttc cgtggattgg ggttctcttc ctggcccttg
     1741 ccttcctgga gcaccagacc aagcgcccgc tcgagatcgg attcgaccac gagcaggtgg
     1801 tcattaatac gctcgtacgg cagcggcatg cctggtccgc gtttaataat gtgctcctga
     1861 aagacgggct tctgaccctc gacttcaaga acaatcgcct cctccaaaag gaagtggtgg
     1921 aggacgagga cgaagaaaat gccgatgagg aagaattcaa cgcctattgc cgcgaacggc
     1981 taacagccgc ttatggaaaa taagcgcttt gccgtctcgg cagaaaccgc gatctctgcc
     2041 acaatcttca attcgcggaa ctcgatcaaa ttcacgaatt ttgcaggcat tatattggcc
     2101 gaatcgccat tcaaccggga agctgatgcc aacatcacca tttattctat actccgacgg
     2161 gaagggaaat atcttcgaag accgatcgct atatgcgacc ggccgcagcg gctgggatgc
     2221 cattcccgtg ccggcggaag actggatcgg cctgccggac ggcggttcgc tgtatgagct
     2281 cccgggcaga aagggtatcg gcctcgacgt ccggaccggc gagatgcggc tttgcgaaaa
     2341 aggttgggcc gttgccgctt ttgttccgcc tgcgcatacc ggcctctacc tcgctgccta
     2401 tgagaccggg gagaacgccc ctaccctgcc gctcttttgc tacaccgcag ccggttggaa
     2461 cgatggcaag ttcctcgttc cggccgtcag gatcgaacag gacatccgcc aggactgcgt
     2521 gggctacgac tgggatgtca tccattccgg ggtcgaccgg ctgctgaaag cctatccgca
     2581 aaaccggctg gtcaggcatc tggcgcaaaa ttgctgcctg acctatcatt gccccgcggc
     2641 ccggaattat tttatgggcc ggtgggaatg cccggtgccc tcatcgccgg cctgtaacgc
     2701 caactgcatc ggttgcattt ccttccagcc gcaggaggaa acgatcccgt cgacccagga
     2761 ccggcttacc ttcaggccca ccgcggagga gatcatcgaa ttcaccgtac cgcatctgca
     2821 gaccgcccgc tatcccatcg tgagctttgg ccagggctgc gaaggcgagc ccctgctgat
     2881 gtgggaaacg cttgcggagg ccatccgcgg aatgcggcgt cataccgggc gcggcagcat
     2941 caatatcaat accaacggaa gcaggccgga tgccgtgcgg gcgctttgtg aggccggcct
     3001 cgattcgatc cgtgtgagcc tcaactccgc gcggccctcc atctataccg cctattaccg
     3061 gcccaacaac tatcgtttcg cggatatcat cgaaagtctc cggattgtcc gcagctatgg
     3121 cggttgggca tccatcaact attttgtgtt tcccgggatg accgacagca cggaggaata
     3181 tgaagcgctt cggacgctga tccgcgaaac ggacctgacc atgatccagt ggcggaactt
     3241 taatatcgac cccgattggt acctcggaaa gatcggggtg acggagacag gagaattcct
     3301 tggcgtccgc cagttgatgg agctgatccg ggaagagttc cccgcagtgc ggtttgggta
     3361 tttcaatccg cccatggaac ggatccgcgg gcgctacgaa gctgacttcg cccattaact
     3421 cttatgtcaa aagtcatcat catcggggcc acttccggca tcggccgcga actggcgagg
     3481 cggtatgccg cgggaggcca tcttgtcggc gccaccggcc gccggcagga actgcttgcc
     3541 acccttgcag cggaatatcc cggccgggtc gtaacggcct gttatgacgc aaccggccgc
     3601 gacgcgatat cggaatccgt cagcctggca gaacgcctgg gcggcatgga cctgcttatt
     3661 tacaatgcgg gctggggcga attctcggcc ggcctcgatt gggacatcga caagcaaacc
     3721 gtcgatatca acgtgaatgg ctttttgatg gcggtccact tcggttggga ccatttcatc
     3781 cgccagggcc atggccatct cgtcaccatc tcgtccgtcg cgggtaaccg cggcaaccgg
     3841 tacacgcctg cctacagcgc ggcaaaatct tttcaaagcg tctatttcga aggtttgtat
     3901 atcaaggcgc gccgcctcaa tatcccgctt tatgtcaccg atatccaacc cgggttcgtc
     3961 aacactcaac tggcgaaggg cccacgcttt tgggttgcgt ccgtggatca ggcggcggat
     4021 cagatcgtcc gcgccatcgc gcggcgccgt cgcaaggctt atatcacccg gcgatggtgg
     4081 atcgtcgcga aacttttccg ctggatccca ggatgggttt actaccggct ttgactgcct
     4141 ccgcctgcgc tcgcggggtt tggcactcca attgcaacat tggcaaaaga aacgttcctt
     4201 gtatgaaaag aaaccagcta atcgcgatat ctgcgctaag cgtcgtcctc gccgccacta
     4261 ccgtctatct catccggaag agccggcaaa accagcgcaa aagagtggtc gcgaatgcag
     4321 gctatgaaat ggcatatgac atccactatc cgatgaagta ttccacccgc cggcgacctg
     4381 ctgccggtta aaatttaacg ttttatcaag gataacccta ataatttcac aacctcgtgg
     4441 ggcgttatcg acgataaatt tgataaattc gcgtcaggat gtcgacgtat ctacctgatt
     4501 cattcgaaag gcaaaaaaac cttaaagctt ccggttacac cgcgaccatt gttgcggccc
     4561 tgatcctgtt actgtatatc agctggacgg tgccgacaga agtccctccc gtgcaggaag
     4621 agggtatcga ggtcaatctg ggtaacagcg acaaggggat gggcgtggac caaccctacc
     4681 ttccgggtaa gccggcggcc gaaaacaagg aaaaatatac gccgcccaaa caggcggtcg
     4741 tcgaaaggca gccggcaaag gacgtcgaga cggacgataa ccaaaaggac gacgcgccgg
     4801 tcatcaagaa accggcagtc accaggcccg atgccaccaa acttcccgac aaatcggtcg
     4861 ccaaaaaagt ctcaaggccc gtcaaacagg ccgagcccga gccggtgaaa cctgtcgtgc
     4921 gccatgcgaa ggcgctgatg gggacgataa gcggtaccgg caacggcggc aacgacgcgg
     4981 atgacttcaa acccggcggc aaccagggca tcgcaggcgg ccggggtgat cagggcgcgc
     5041 cgggcggcga tccgaattcc aataattaca ccggcggagg acatgggaat ggaattgcca
     5101 tctcgagagg gctggaagga cggcatatcc ttactgcacc ttcttttacg ggggacttta
     5161 actacaatgc aaaggtggca gtcgacgtcc acgtcgacga gaacggcaat gtcaccagcg
     5221 ccgaatatca gatgcgcggc tccacgacat cggccgacaa ttacgttaca accgccaaac
     5281 aaaaagcccg cctcgtgaaa ttcaacccgg ggtcgggcga gtctgtcggt
//
LOCUS       DATGKE010000470         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_215356, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000470 DATGKE010000000
VERSION     DATGKE010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215356"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2738
                     /locus_tag="VKU83_08150"
     CDS             <1..2738
                     /locus_tag="VKU83_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLI93565.1"
                     /translation="PMIMVDGLQINATDINSLDLSNIERVEVVQGAASAAIYGAQGAN
                     GVIQIFTKKGHRGAAVINFSTSYASNSYINSGHMKKAELNAWKTDASGNFIDKNGNPV
                     SFDQYGDLDGLSYQYGGPTRYGILNTLNTDSHPYGTNLKYYDHFKQVFQTGNTYNNVL
                     SISGATDKTDYNISAANNHTLTPVLQAGYLDRSNLSANLGVELFKGFRLRSTTQLIYT
                     KNTIVPGLGGPGGFGYGQGNSLGNNGNVFSFLNTSPFFDLNYKLADGTHPAYQVASFV
                     SINAFNPYYTKEYASGLDNKIDVVQSFDANYKMNRFVELDAKYGINYRTENSRWTYAN
                     QSNNINTTYQYDWAGEFAPDWNGEIDDWQYNTTFQNFIGSAFFRTDFQNDFHSKLPIT
                     TSTQVAFDYRKRKYTEYDSYGIGLATSPPINMTHTASQAAANDYVEPFITFGYLVNQT
                     VDFGTWGGVAGGFRSDYSSAFGQGSKPFTFPHVNGYFIPSSFDFWNNGKIGNIFSLFK
                     LRAAYGEAGIQPGPFDRYPSLSGGDLGTHQVYSFNANSKNPFLNVEVSKEFEVGTDLT
                     IPANKTGSVFSAVNLSFTYWHKRSSDVIYNVDVPLSTGSIQFLDNAFDLHSQGYQFSV
                     NTPVVQTRNFTWNFTTNWAHQISMIDKVKGNQPIPVGPIDGNSLYVYLVPGYRIGQLY
                     GYKTIHSFDQTYKDGKTKYFPNGNNNGQYTMVEGNVVDTGTMAIQFTAEKYPLGNTDP
                     KFNAAFINEFNYKDFLTFSFQFDWIDGSHIYSETKEWMYRDGIDGDFAKTVNIAGKVG
                     AFTAYRASAYYALWGSLHGVGNEAPKDYFLYPASFLRLRNVALALDFARLYKIRYVKR
                     LQLVLSGRNLWTQTKYPGMDPEISSLTPNSSYTRGVDNSSIPNLKSYQASLNIGF"
     gene            2764..4449
                     /locus_tag="VKU83_08155"
     CDS             2764..4449
                     /locus_tag="VKU83_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI93566.1"
                     /translation="MTKDKLYNIIMIIGVLATAACSKKQLQVGDPNDPTLVGNVTDEA
                     GLIALAQGGVYLDGFANGDGWLGNSYFSLPRAYLELMADNIGADASNNQITTVGYPTY
                     YILDDGTKIVSNTASTHPQIDLLRQYNILGGSGNNPMYYVWQNMYALNAACNSILKVA
                     PTIKLSGDAASRLATIKAWCYFWKGYAYSQIGTTYFSGLIQDDPNNITNNHYLPKDSI
                     LAQSNYYLNLCSTTIGGISSASDFATVLGELIPDFCQVGNGGVLTVTMWQHNINTLLA
                     RNILLNKLSPYVNGDPGASISKSSMTSMTSADWNSVLSLATNGIQPGDYVFTGRSLAT
                     NGFFTASTGNVGAETTGPNIASTFKIGVRYIANFKPGDQRLAQNVVMSSVYNNPFYGT
                     PYDIVDSLQQSNTGIYVLGNKQVGEYEVIIVGSLEENLLMLAEANIRLGNIDAGLAFV
                     DQERTLQGAGIAPVSGTGLTLAQAMKELTMERRAALIFRGLAYFDARRWGWTYDISVG
                     GGVYGQPLYNTDGTYDTNVTINYNFMDYWDVPADESQLNPPGTGSSPVTNPNY"
     gene            4454..5164
                     /locus_tag="VKU83_08160"
     CDS             4454..5164
                     /locus_tag="VKU83_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93567.1"
                     /translation="MRTLLLFGLILSGALSRDVHAQDGFVAQTGAPLSYNVFDRNGRM
                     FVNPSPDVAGSPFYADDWRLGTLVAMDNYRYDSVKLRLNLQSEEVHVLDTNRNEIALA
                     KGYIKEVVLPGKFNGKAGGARFKCGFPAIDALDKYTFYEVVSEGKCSLLHSIRKVISQ
                     QKDDLSGEVTKEYQLYDEYYVYDGQRMQRVKKDKAFILAALNGKRDQIEAFMDANKLK
                     ARSVDDIKRIIDYYNTLP"
BASE COUNT         1340 a         1380 c         1297 g         1197 t
ORIGIN      
        1 agcccatgat aatggtcgat ggcttgcaaa taaacgcgac cgatatcaac tcccttgacc
       61 tcagcaacat cgagcgcgtc gaagtagtac agggcgccgc ttccgctgcg atctacggag
      121 cgcagggcgc caacggggtc atccagatct ttacaaaaaa gggccatcgc ggcgcggccg
      181 ttatcaattt ctccacgagc tatgcctcca actcctatat caatagcggg cacatgaaga
      241 aggctgagct caacgcctgg aagacagatg ctagcggcaa ttttatcgat aagaatggga
      301 acccggtatc tttcgatcag tacggtgatc tcgacgggct ttcgtaccaa tacggcggac
      361 ccacgcggta tggcatcctc aacactttga ataccgacag ccatccgtac ggcacgaatc
      421 tgaagtatta tgatcatttc aaacaggtgt ttcaaaccgg gaatacctac aacaatgtgc
      481 tcagcatttc gggcgcgacc gataagaccg attataatat ctccgccgcc aataaccata
      541 cgcttactcc ggtgctacag gccgggtacc ttgacaggtc caatctctct gcaaatctcg
      601 gggtagagct cttcaaggga ttcaggcttc gctccacgac ccagttgatc tataccaaga
      661 acaccattgt gcctggtctg ggcggccccg gaggtttcgg ttacgggcag ggcaattccc
      721 tcggcaataa tggaaatgtc ttttcgttcc tgaatacttc gcccttcttc gacctcaatt
      781 acaaactggc ggatggtacc cacccggctt accaggtggc gtctttcgtc agtatcaatg
      841 cttttaaccc gtactatacg aaggaatacg cgagcggcct tgacaacaag atcgacgttg
      901 tgcaaagctt tgacgccaac tacaagatga accggttcgt agagctcgac gcaaaatatg
      961 gcatcaacta ccgcaccgag aattcccgct ggacttatgc gaaccagtcg aacaatatca
     1021 atactacgta tcaatatgac tgggccggag aatttgcacc cgactggaac ggagaaatag
     1081 atgactggca gtataacacc actttccaga actttatcgg gagcgccttt ttccgaacgg
     1141 attttcaaaa cgacttccac agcaaacttc cgatcacgac ttccacccag gtggcgttcg
     1201 actaccgcaa gcgtaagtac accgagtatg attcgtatgg aataggattg gcaacttcgc
     1261 ctcccatcaa tatgacccat acggcaagcc aggcggccgc gaatgattac gtagagccat
     1321 ttatcacctt cggctacctg gtcaaccaaa ccgttgattt cggaacctgg ggcggtgtgg
     1381 ccggcgggtt ccgcagtgac tattcctccg cgttcggtca gggttccaag ccctttacgt
     1441 tcccgcacgt gaatggttat tttataccct cttcatttga tttttggaac aacgggaaga
     1501 ttggaaatat tttttcatta ttcaagctca gggccgccta cggtgaggcc ggcattcagc
     1561 cggggccatt cgaccgctac ccgtcactct cggggggcga cctcggtacg caccaagtct
     1621 atagtttcaa cgcgaacagc aagaatccat tcctgaacgt agaggtatca aaggaattcg
     1681 aagtcgggac tgatctcacg attcccgcga ataagacggg ctcggtattc agcgccgtta
     1741 acctctcttt cacttattgg cacaagagaa gctcggatgt aatctataac gtagacgtac
     1801 cgctttctac cggttcaatc cagttcctgg acaatgcttt tgaccttcat tcgcaggggt
     1861 atcagttctc tgtcaacacc ccggtggttc aaacccgcaa tttcacctgg aactttacta
     1921 ccaactgggc gcatcagata tcgatgatcg acaaggtaaa aggcaaccag ccgattcccg
     1981 tcggacctat cgatgggaat agtctctacg tttacctggt tcccggctac cggatcggcc
     2041 agctttatgg gtacaagacg atccacagct ttgatcagac atacaaggac ggaaagacaa
     2101 agtacttccc gaatggcaac aataacggcc agtacacgat ggtggaaggc aatgtcgtgg
     2161 acaccggcac catggccatc caattcaccg cggaaaaata tccccttgga aacacggatc
     2221 ccaaattcaa cgcagcgttt ataaatgaat ttaactacaa ggattttctg accttctctt
     2281 tccaatttga ctggatcgat ggctcccata tctacagtga gacgaaggaa tggatgtacc
     2341 gtgatggcat cgacggcgat tttgccaaga cagtaaacat tgccggaaag gttggggcgt
     2401 ttactgctta ccgggccagc gcttattatg ccctgtgggg cagcttgcac ggcgtaggca
     2461 atgaagcgcc gaaggactat ttcctgtatc ccgcgagctt tttgcggtta cggaatgtcg
     2521 ccctcgccct ggatttcgcc cggctgtata agatcagata tgtcaaaagg ctccagctgg
     2581 tgttgtcagg ccgcaacctg tggacgcaaa ccaaataccc gggaatggat cccgagatca
     2641 gttcgcttac acccaactcc tcgtacacaa gaggcgtgga caacagttca attccgaacc
     2701 tgaaatcgta tcaggccagc ctgaatatcg gtttctaatt gttgaatcat aaaaaagaaa
     2761 gtcatgacta aagataagtt gtacaatata ataatgatca ttggagtcct tgcgactgca
     2821 gcctgtagta aaaagcaact gcaggtcggc gaccctaacg atccgacact ggtcggaaac
     2881 gttactgatg aggcaggctt gatcgcactc gcgcagggag gtgtctatct cgacggtttt
     2941 gcgaacggcg acggctggct ggggaatagc tatttttcac tgccgcgcgc atacctcgaa
     3001 ctgatggccg ataatattgg cgcggatgca tcgaacaacc agatcacgac ggtcggttac
     3061 ccgacgtatt atatcctcga tgacggaaca aagatcgtct ccaacacagc cagcacgcac
     3121 ccgcagatcg atctccttcg gcagtataat attctcggcg ggagcggcaa taacccgatg
     3181 tattatgttt ggcagaacat gtatgccttg aacgccgcct gtaattcgat cctgaaggtg
     3241 gcgccaacca taaaactttc aggggacgcc gcaagccggc tggctaccat caaagcctgg
     3301 tgctatttct ggaaagggta tgcctactcc cagatcggca ctacgtattt ttccggactg
     3361 atccaggacg atcccaataa cattacaaac aaccactacc tgccaaaaga ctcgatcctt
     3421 gcccagtcca actattatct taacctctgt tccaccacca ttggggggat cagcagcgcg
     3481 agcgactttg caacggtgct gggcgagctc attcccgact tctgccaggt cggcaacggc
     3541 ggtgttctga cggttaccat gtggcagcac aatatcaata cgctgctggc aagaaatatt
     3601 cttctgaata aactttcccc gtacgttaat ggagacccgg gagcgagcat ttcaaaatcg
     3661 tccatgacca gtatgacttc cgcggactgg aacagcgttc tgtcattggc aaccaacggt
     3721 atccagcccg gtgactatgt ctttaccggc agatcgctgg caacaaatgg tttctttaca
     3781 gcgagcaccg gaaatgtcgg cgccgaaact accgggccta acatcgcctc aacattcaag
     3841 atcggcgttc ggtatattgc gaattttaag ccgggagatc aacggctcgc tcaaaatgtt
     3901 gtgatgagca gcgtctacaa caatcccttc tatggcaccc cttacgacat cgtagattcc
     3961 ctccagcaaa gcaataccgg gatctatgtc ctcggaaaca aacaggtagg tgaatacgag
     4021 gttattatcg taggaagcct cgaagagaac cttctgatgc tggcggaagc caatattcgc
     4081 ctcggcaata tcgatgccgg gcttgctttc gtcgaccagg aacggacgct gcagggagcc
     4141 ggcattgccc ctgtttcggg gaccgggctg acattggcgc aagccatgaa ggaacttacc
     4201 atggaaaggc gggctgcctt gatctttcgc ggccttgcct actttgatgc ccggcgctgg
     4261 ggctggacat atgacatcag cgtaggcggc ggggtgtacg gccagccatt atataacacc
     4321 gacggaacct atgataccaa cgtgaccatc aattataact ttatggacta ctgggatgtg
     4381 ccggcggacg aaagtcagtt gaatccaccc ggaaccggca gttcgcccgt cactaacccg
     4441 aattattgac cacatgcgaa cgctgctgct tttcggattg atcttgtcag gcgctttatc
     4501 ccgcgatgtt cacgcgcagg acggattcgt cgcgcagacc ggagctccac tgtcgtacaa
     4561 tgtttttgat cggaatggcc ggatgttcgt aaacccgtca cctgacgttg ccggcagtcc
     4621 cttctatgcg gacgactggc ggctgggcac gctcgtggcg atggataact atcgttatga
     4681 cagcgtaaag ctgaggctta accttcagtc cgaagaagtg catgtactcg acacgaatcg
     4741 aaacgagatc gcgttggcaa aagggtatat aaaggaggtt gtgttgccgg gtaagttcaa
     4801 cggaaaagcc ggcggcgccc gcttcaagtg cggattcccc gcgatcgacg ccctcgacaa
     4861 atacaccttt tacgaagtgg tttccgaagg caaatgctcg ctgcttcatt ctatccggaa
     4921 ggtcatttcg cagcagaaag acgacctgtc aggtgaggta acgaaggaat accagcttta
     4981 cgacgagtat tatgtttacg atggccaaag gatgcagcgg gtaaagaagg ataaggcctt
     5041 catcctggct gccctcaacg gcaagcggga ccagatcgag gcttttatgg atgcgaataa
     5101 gctaaaagcc agatccgtcg acgatatcaa acggatcatc gactattata atacacttcc
     5161 ctgaataagg gcaatggata aaggcggcaa tcttatcgcc cctgaaaaat gtgt
//
LOCUS       DATGKE010000471         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1224094, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000471 DATGKE010000000
VERSION     DATGKE010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224094"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VKU83_08165"
     CDS             <1..451
                     /locus_tag="VKU83_08165"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLI93568.1"
                     /translation="IAAIWYWLGVHAIVYLLLSFFFSVGLHPLGARWVQEHYLTDDEH
                     QETHSYYGILNTVAFNVGHHNEHHDFPSIPWNKLPQIRRAAPEYYDTLYYHTSWTRLF
                     FRFLFDREISLYHRIIRKDRGKVALTDQSTPDRDLLAAAPKAPVTSE"
     gene            560..1249
                     /locus_tag="VKU83_08170"
     CDS             560..1249
                     /locus_tag="VKU83_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLI93569.1"
                     /translation="MRTTVFRRTIALFLILTVTSLLTAFKPKTQKVIFFGDSITYMGA
                     KPGGFIMRMKDALDKKNLSSDYDLVGAGIGGNKVYDLYLRMDDDVLAQNPDIVFIWIG
                     VNDVWHKATFGTGTDPDKFEKFYTAIINKLLDRHIKVILVTPAVIGEKTDFTNRQDGD
                     LNAYSQIVRNLAQKFHTGLVDLREIFHTYELSNNPGNKEQGILTVDRVHLNDAGNELV
                     AENFLTALLSK"
     gene            complement(1309..1614)
                     /locus_tag="VKU83_08175"
     CDS             complement(1309..1614)
                     /locus_tag="VKU83_08175"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93570.1"
                     /translation="MKVHESPYINRRKKTIEFYVHHLNSRAAAHICLAGSFNHWSPDE
                     LKLQPAEDEGWKISIPMLPKGKYYYKFFIDDKVWMEDIENRLREPDGVAGWNSILTV"
     gene            1866..2558
                     /locus_tag="VKU83_08180"
     CDS             1866..2558
                     /locus_tag="VKU83_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI93571.1"
                     /translation="MAETRILLVEDEKKIAESLRKGLSEHRYEVDVAYDGAIGRKLFD
                     QKMYDLAILDINLPLMNGYELARYIRQSNDEILVIMLTAMNTTEDKIEGFEAGADDYI
                     VKPFDFQELLVRIRALLKRLHHQPAAGANVLKVADLTMNLDSKEVSRDGRIIPLTAKE
                     FQLLEYFIRNKNKVVSRVDIALNVWDIDFDTKTNVIDVYVNFLRKKIEKDFASKLIYT
                     QVGMGYVLKENP"
     gene            2592..>3313
                     /locus_tag="VKU83_08185"
     CDS             2592..>3313
                     /locus_tag="VKU83_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLI93572.1"
                     /translation="MLLFTLLVTGIISLFTGSIYYFARAEREAAFDRRLKARANYNSQ
                     LYALMGDSAFYLLHRMDTAAMVGTVGSRSIGIFSETGSVIYLFNMPGTIPLSFSDSFF
                     REVKEKGEKDFKMDNRDAVALDKVTGRRNFIVVSVGHDDDGLERLDTLKSILLVGLVT
                     SICLTALVSFLFARQLLRPVALIIREVNEISSFDLSRRIQAGSGHDEMSQLANTFNEL
                     LARLQESFAIQRRFISNASHELS"
BASE COUNT          789 a          918 c          814 g          792 t
ORIGIN      
        1 tatcgctgcg atctggtatt ggctcggggt gcatgcaatc gtctaccttt tgctaagctt
       61 ctttttttcg gtggggcttc atccgcttgg cgcccgctgg gtccaggaac attatctgac
      121 cgacgatgag caccaggaaa cgcatagcta ttacggcatc ctgaacaccg tcgccttcaa
      181 cgtgggccac cataacgaac atcacgattt tccgtcgatc ccctggaaca agctcccaca
      241 gattcgccgg gcggccccgg agtattatga caccctctac tatcatacgt cctggaccag
      301 gctcttcttt cggttcctct ttgaccgcga gatcagcctg taccaccgca ttattcgcaa
      361 agaccgtggc aaagtagcgc ttacggacca gtcgactccg gaccgggacc ttctcgctgc
      421 ggccccgaag gcccctgtca cgtccgaata gctaccggtg ttgaaacggc cagacgggtc
      481 aggactgccc gaaggggcaa aatgagaatc tctgaataat ttgccgtacg tttgacttca
      541 aattcgaaaa tcttacgcca tgcggaccac tgttttccgt cgaaccatcg ccctcttcct
      601 catcctcacc gtaaccagcc tgctgacagc tttcaagcca aaaactcaaa aagtaatttt
      661 cttcggtgac tccatcacgt atatgggcgc caaaccgggc ggctttatta tgaggatgaa
      721 ggatgccctt gacaagaaga acctgtcctc cgactacgac ctggtcggcg cggggatcgg
      781 cggtaataag gtttatgatc tgtacctccg catggatgac gatgtactgg cgcaaaaccc
      841 cgatatcgtt tttatctgga tcggcgtcaa cgacgtctgg cataaagcca cattcggcac
      901 cggcaccgac cccgacaagt tcgaaaaatt ctataccgcc atcatcaaca agctccttga
      961 ccgccatatc aaagtcattc ttgttactcc ggcggtgatc ggggaaaaga ccgattttac
     1021 gaaccggcag gacggcgatc tgaacgccta ttcccagatc gtccgcaacc ttgcgcagaa
     1081 attccatacc ggcctcgtgg acctgcgcga gatcttccat acctatgagt tgagtaacaa
     1141 tcccgggaac aaggagcagg gcatcctgac agtggaccgc gtccatctga acgacgccgg
     1201 gaacgaattg gtcgcggaga attttctgac cgcactcctt tcgaaataag gcaaaacaag
     1261 agcggccggc gcattgcgcc ggccgctcca gacggaacca atattgtctt aaacagtcaa
     1321 tatgctattc catcccgcta cgccatccgg ttcccgtagc cggttttcaa tgtcctccat
     1381 ccagaccttg tcatcaatga agaacttgta atagtacttg cccttcggca acataggaat
     1441 ggaaatcttc cagccttcat cctcggccgg ctgtaacttc aactcatccg gagaccagtg
     1501 attaaaggag cctgccagac agatgtgggc tgctgccctg gaattaagat gatggacgta
     1561 gaattcgatc gtcttcttac gccggttgat gtacggcgac tcgtgtactt tcatagcaac
     1621 cccctttttt aagatttaaa tcagccttca gtagggcgga acacctagca agcgatggtt
     1681 ttgtaaatag aggtttcggg cgggtataca aaatttacat ttttttcccg gatatttcaa
     1741 caaagacgaa attttttttg ctaaatcggt ttatggcgta ctactgttta attccttttt
     1801 aatcgggcat ccgatgtccg ctcgcttgga ttagataaat ttaagcctca cctttagggc
     1861 tgattatggc agaaaccagg attttattgg tcgaagatga aaagaagatc gccgaatccc
     1921 tgcgaaaagg cctttccgaa caccggtatg aggtcgatgt agcctacgac ggggccatcg
     1981 gccggaaact tttcgatcaa aaaatgtacg acctggccat tcttgacatc aacctcccgc
     2041 tgatgaacgg ctacgagttg gctcgctata tccgtcagtc gaatgacgag atcctggtta
     2101 tcatgctgac cgccatgaac accaccgaag acaagattga agggtttgaa gccggcgccg
     2161 acgattatat agtcaaaccc ttcgacttcc aggaactcct tgtccgcatc cgcgccttgt
     2221 tgaaacggct gcaccaccag ccggccgccg gcgccaatgt cctgaaagtg gcggatctga
     2281 ccatgaacct cgacagcaag gaagtgagcc gcgacggtcg gattatcccc ctgacggcta
     2341 aggagttcca gctgctcgag tatttcatca ggaataagaa caaggttgta tcccgggtcg
     2401 atatcgccct caacgtctgg gatatcgact tcgataccaa gacaaacgtg atcgatgttt
     2461 atgtcaattt cctgcgaaaa aagatcgaaa aggactttgc gtcgaaactt atctacacgc
     2521 aggtcgggat gggttacgtc ctgaaagaaa acccgtaagg ccccttacat ttgaagatac
     2581 ggttcaaaat aatgttgctt tttacgctcc tggtgaccgg gatcatttcc ttgttcaccg
     2641 ggtcgatcta ctacttcgcc cgtgcggagc gggaggctgc attcgaccgc aggctgaagg
     2701 cgcgcgccaa ttataacagt caattatacg ccctgatggg cgacagcgcc ttctacctgc
     2761 tccaccggat ggacaccgcg gccatggtcg gcacggtggg atcccgaagc atcggcatct
     2821 tttcggagac cggcagcgtc atctacctgt tcaatatgcc gggtacgatc cccctttcgt
     2881 tttccgattc ttttttccgg gaagtaaaag aaaagggcga aaaagacttt aagatggaca
     2941 accgcgatgc ggttgccctg gataaagtga cgggcaggcg caatttcatc gtagtgtcgg
     3001 tcgggcacga cgacgatggg ctcgagcggc tggatacgct taagagcatc ctgctggtag
     3061 gcctcgtgac cagcatttgc ctgacggccc tggtcagttt cctcttcgcg cgccagcttc
     3121 tgcggcccgt cgccctcatc atcagggagg tcaatgagat ctcttctttt gacctctccc
     3181 gccggatcca ggcggggtcc gggcacgatg agatgagcca gctggccaat actttcaatg
     3241 aattgcttgc ccggcttcag gaatccttcg caatccaacg ccgtttcatt tccaacgcct
     3301 cccacgagct ttc
//
LOCUS       DATGKE010000472         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_736974, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000472 DATGKE010000000
VERSION     DATGKE010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736974"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VKU83_08190"
     CDS             complement(<1..1155)
                     /locus_tag="VKU83_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93573.1"
                     /translation="MNHLYRSLLLAVLLFPLALHAQQLDSMLNIYANDYAQEKVYLQL
                     DKQAYRPGETVWFKAYIFSGAGPSAMSHNFYAELSDPSGTILQRKVYPVIESSAAGNF
                     DLPKGISYPYLHIRAYTAWMTNFDSAFYYEKDLRLDDGKVDRARAAGLAPVQTRMQFF
                     PEGGERVAGVGTTVAFLVDDQFGRPVVADGVIENRDGNQVLGFASAHDGMGKFVLPAD
                     AGDSLTAVWKDEEGAAHRTPLPAARRSGIVLRAEPANQKVLFSVTRSAGSDSRLRRLT
                     IIGHMHQHPVYQARISLEDHAESGGTIPTGTLPSGVLVLTVFDANMMPLAERAVFVNN
                     HDYLFSPRLSVQDKGVTRRSKNTLVIDVPDTVTSNLSIAVTDASADGEFRY"
     gene            1239..2750
                     /locus_tag="VKU83_08195"
     CDS             1239..2750
                     /locus_tag="VKU83_08195"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HLI93574.1"
                     /translation="MHLTKSTRDLYGDYVDRMQKIADIKYAAAVLEWDQETYLPPKGA
                     EARSRQLASLSEIAHEWLTDPALGALLEELQQRGDLEEDERRNVQLSLEDYTKQKKFT
                     PAFVRRLSEACSRSFHSWVRARHDNAFRIFQPDLEDVVMLKRQQAELAGYRQHPYDAL
                     LDDFEKGCTTGLLDQVFDSVREPLRDLLGRIAARPQVDDHFLYGDFPRDLQWNFGLDI
                     IRQLGFDFGAGRQDLSEHPFTTSFNARDVRITTRIDDRNFNYMTWSCIHETGHALYEQ
                     GLPPQSYGLPLGEAASLSIHESQSRLWENHVGRSLGWWQTIYPRLQQAFPDRLARTDL
                     QAFYKGINKVCPSLIRTEADELSYHLHVMIRYQLERSLTEGSLQVADIPGFWNASYSK
                     WLGINPPDDKQGCLQDVHWSHGSFGYFPTYSLGSFYAAQLFHSAVNQDIDIEPAMREG
                     NTGPLLHWLRGKVHRHGRRYTSEELCKKATGKALDIRFFLDYLLEKYVIIYDF"
     gene            2768..3181
                     /locus_tag="VKU83_08200"
     CDS             2768..3181
                     /locus_tag="VKU83_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93575.1"
                     /translation="MKQIYPLLLSGILLVSCKKFIQNQAEKQVISDVTNGLWYVTGYE
                     QNDSNITAAFSGYVFKFNANNTVTGILGTDSVPGQWLVNVNARTIASDFPGAGYPLDL
                     LNETWYITDSYTDSVAATSTDSVNHTSNFLQLKKQ"
     gene            3282..>3548
                     /locus_tag="VKU83_08205"
     CDS             3282..>3548
                     /locus_tag="VKU83_08205"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLI93576.1"
                     /translation="MNRKLFMLAGCLLVLGMAVHAQAPVAQFTTAGGVTTGCAPLVVQ
                     FKDQSTNNPTSWEWDFGDGQTSSYQNPNHSYPTAGTYTVTLIARN"
BASE COUNT          830 a         1023 c          904 g          791 t
ORIGIN      
        1 gtaacggaat tcgccgtccg cgcttgcatc cgtaacggcg atggacagat tggatgtcac
       61 cgtatccgga acatcgatca ccagcgtatt cttacttcgc ctggtcacgc ccttgtcctg
      121 aaccgacagc cggggactga aaaggtagtc gtggttgttg acaaagacgg cccgttcggc
      181 cagcggcatc atgttcgcat cgaatacggt caacaccagc acgccgctcg gcagggttcc
      241 ggtgggtatg gttcccccgg actcggcatg atcctccagg ctgatcctgg cctggtacac
      301 ggggtgttga tgcatgtgcc cgatgatggt cagtcgccgc aaccgggagt cgctgcctgc
      361 cgatcgcgtt acagaaaaca ataccttctg atttgccggt tccgctctca aaacaatgcc
      421 cgaacgccgg gcggccggta aaggtgtccg atgcgctgca ccttcctcat ctttccatac
      481 cgccgtcagg gaatcgccgg catccgcagg taaaacgaat ttccccattc cgtcatgcgc
      541 ggatgcgaat cccagcactt gattgccgtc gcggttctct atcaccccgt ccgccactac
      601 cggccggcca aactggtcat ccaccaggaa agccaccgtt gtcccgacgc ccgcaacccg
      661 ctccccaccc tcaggaaaaa attgcatcct ggtttgcacg ggcgcgaggc cggccgcgcg
      721 cgcccggtcg accttgccat catcgagacg taaatccttc tcatagtaaa acgcagagtc
      781 aaagttcgtc atccacgcag tatacgcccg gatatgcaaa tagggataac tgatgccctt
      841 cggcaggtca aagttacccg cggcgctcga ttcaataaca ggataaactt ttcgctgcag
      901 aatggtcccg gaaggatccg acaactcagc ataaaaattg tgactcattg cagaggggcc
      961 ggccccggag aaaatataag ccttgaacca aaccgtctca ccgggccgat aggcttgctt
     1021 gtccagttgg agatacacct tttcctgcgc gtagtcattt gcataaatat tcagcatgga
     1081 atccaactgc tgggcgtgaa gggccaaagg gaacagcaaa acggcgagca aaaggctgcg
     1141 gtataaatgg ttcatgatcc taatttattc aattagatcg gcaaaatgga tatactttcg
     1201 gcgtgctttt cattttttaa tgtggaaata atgttaagat gcatttaaca aaatcgacgc
     1261 gcgatctcta tggcgactac gtcgaccgga tgcaaaagat cgcggatatc aaatacgcgg
     1321 cggccgtact tgaatgggac caggaaacct atttgccgcc caaaggcgct gaggcgcgga
     1381 gcagacagtt ggccagcctt tcagagattg cgcatgagtg gctcaccgat cccgcgcttg
     1441 gcgctctcct ggaggaattg cagcaacgcg gtgaccttga agaagacgag cgccggaatg
     1501 tccaactcag ccttgaagac tataccaaac agaaaaaatt cactccggct tttgtccgca
     1561 ggctcagtga agcctgctcc cgcagcttcc actcctgggt ccgtgcgcgc catgacaatg
     1621 cgttccggat cttccagccg gatctcgagg acgtggtcat gctcaaaagg caacaagcgg
     1681 agcttgccgg ctaccggcaa cacccctacg atgccctgct cgacgatttt gaaaagggct
     1741 gcacgaccgg gttgctcgac caggtcttcg atagcgtccg ggaaccgctg agggacctgc
     1801 ttggccgcat cgccgcccgc ccgcaggttg atgaccattt cctgtatggt gactttcccc
     1861 gcgatctgca gtggaacttt ggtctggaca ttatccggca actcggtttc gacttcggcg
     1921 ccggccgcca ggacctgtcg gaacacccgt ttaccaccag ctttaacgca agggacgtcc
     1981 gcattacaac gcggatcgac gaccggaatt tcaactacat gacgtggagc tgcattcatg
     2041 aaacgggtca cgccctttac gagcaaggtc tgccgcctca atcttacggc ctccctctcg
     2101 gcgaggccgc ctccctcagc attcatgagt cgcagtcccg gctttgggaa aaccatgtcg
     2161 gcaggagcct cggctggtgg caaacgatct atccacggct ccagcaagcc tttccggatc
     2221 gcctcgcgcg aacggacctg caagcatttt ataagggtat caataaggtt tgcccgtccc
     2281 tcatccggac cgaggccgac gagctctcct accatctcca tgtgatgatc cggtaccagc
     2341 ttgaacggag cctgaccgaa ggatccttgc aggtagccga cattcccggg ttctggaacg
     2401 caagttactc taagtggttg ggtatcaatc ctccggacga caagcaaggt tgcctccagg
     2461 atgtccattg gagccacggc agttttggct attttccaac ctatagcctg ggcagttttt
     2521 acgcagcgca gctgttccat tccgctgtaa atcaggatat tgacatagaa cccgcgatgc
     2581 gcgagggcaa taccggcccc ctgcttcact ggctgcgcgg gaaggtacac cgccacggac
     2641 gtcgttatac cagcgaagaa ctgtgcaaaa aggcgaccgg gaaggccctg gatatccgct
     2701 tttttttaga ctatttacta gaaaaatacg ttattattta tgacttttga ccttaataca
     2761 tcgtattatg aagcagatat acccgctgct cttatccggc attcttcttg tatcctgtaa
     2821 gaaattcatt cagaatcagg cggaaaagca agtaatttct gatgtgacca acgggttgtg
     2881 gtatgtgact gggtatgagc aaaatgacag caatataacg gcggcgttct ccgggtatgt
     2941 ctttaaattt aacgcaaaca ataccgtcac ggggattctc ggcaccgatt cggtgccggg
     3001 acagtggttg gtgaatgtca acgcgcggac gattgcttcc gattttccgg gcgctggcta
     3061 cccgctcgat ctgctgaacg aaacctggta tatcacagac agttatacag atagcgtcgc
     3121 agcaacgtca acggacagcg tcaatcacac ttccaatttc ctacaattga aaaagcaata
     3181 atccagtctt cttattggtg ccgctccaat gtcctattca acagccatcg ccgcttagcc
     3241 tgcggttaaa gtgactttac ttttacctaa acgaccctta gttgaacaga aaactattca
     3301 tgctggccgg ctgcctcctg gtgcttggaa tggccgttca tgcgcaagct ccagtcgccc
     3361 agttcacaac cgccggtggc gtaaccacgg gctgcgcccc gctggttgtc caattcaagg
     3421 atcagtcgac caataaccca acttcgtggg aatgggactt cggcgacggc caaacctcct
     3481 cctatcagaa ccccaaccat tcttatccca cggcagggac ctatacggtg accttaatcg
     3541 cccgcaac
//
LOCUS       DATGKE010000473         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1241982, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000473 DATGKE010000000
VERSION     DATGKE010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241982"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1598
                     /locus_tag="VKU83_08210"
     CDS             96..1598
                     /locus_tag="VKU83_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLI93577.1"
                     /translation="MGRKNAIRQTEYNARRTAALQYYTRRHFLKESAMGLGGLALGAL
                     LGCHRTRGSVPVVFDPAHPLEPKLPPFPGKAKNVIYLHMAGAPSQLELFDYKPELKKM
                     DGQDCPESLLAGKRFAFIRGVPKMLGPQARFAQYGESRAWVSEHLPHFSTVVDEVSFL
                     KAVTTDQFNHAPAQLLVHTGSPRLGRPSMGSWVTYGLGTMNQDLPGFVVLTSGGKFPD
                     AGKSVWGSGFLPSVYQGVQCRSVGDPVLFLDDPAGMDRDLRKESIDAINAVNQKEFAE
                     YHDPEILSRISQYEMAFRMQISVPEVMNIQDEPPYIHELYGTSPGKGTLANNILLARK
                     LVEKGVRFVQIFDWGWDSHGTDASLAIDVGFINKCRQVDRAMTALIVDLKQRGLLDET
                     LVIWGGEFGRTPMMENREGKPNPFKGRDHHTDAFTIWMAGGGIKKGHVYGETDEIGYS
                     AILGKVDTFDVQATILNQLGFDHTRFTYDFQGRPFRLTDVKGNVIKEIIA"
     gene            1595..>2120
                     /locus_tag="VKU83_08215"
     CDS             1595..>2120
                     /locus_tag="VKU83_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93578.1"
                     /translation="MINRKQFIAHSTLAATGLLFESFAQPLSFPSVREGFQLKILATD
                     WGFPGSLDEYGAKVRNAGYDGIEIWWSLDPGRQKEVFAMLKKYGLEVGFLCGGSESDP
                     KEHFNHFKQMVEGAARNPTQRPLYINCHSGRDFFSYDENKPFIDLTTALTRETGIRIM
                     HETHRSRILYSAPVA"
BASE COUNT          469 a          566 c          640 g          445 t
ORIGIN      
        1 ggcgccaaca cgacccaact ggccgaaagg gcgtccctcg tcgtcgtctg caatgccctt
       61 ttgaacctcg acgaggtgat cactaaaaat tgattatggg ccgaaaaaat gcaataaggc
      121 aaacggagta caatgcgagg cgaacggccg ccctgcaata ttatacgcgg cggcatttcc
      181 tgaaggagag cgccatgggc ctggggggcc tcgccctggg cgcattgctg ggctgccatc
      241 ggacgcgtgg atcggttccc gtggtttttg atccggctca tccgctcgag ccaaagttgc
      301 ctccattccc gggcaaggcg aaaaatgtca tctatctgca tatggccggg gctccttcac
      361 agttggagct gttcgattat aagccggaac tgaagaagat ggatggccag gattgtccgg
      421 agtcgctgct ggcgggcaaa cgtttcgcgt tcatccgggg cgtgccgaag atgttggggc
      481 cgcaggccag gtttgcccaa tacggcgaga gccgggcgtg ggtaagcgag cacctgccgc
      541 atttttcaac cgtcgtcgac gaggtcagct tcctgaaggc ggttaccacc gatcagttca
      601 accatgctcc cgcgcagctt ctcgtgcata cgggctcccc ccgcctgggg agaccaagta
      661 tgggaagctg ggttacttat gggctgggaa cgatgaacca ggaccttccg ggtttcgtgg
      721 tcctcacgag cggcggaaaa tttccggacg cgggcaagag tgtctggggc agtggtttcc
      781 tgccgagtgt ttatcagggc gttcaatgcc gcagcgtggg tgaccccgta ttgttcctcg
      841 atgatcccgc gggaatggac agagatcttc gcaaggaatc gatcgacgcg atcaacgccg
      901 tgaatcaaaa agaattcgcc gaatatcatg acccggagat cctgagccgg atctcgcaat
      961 atgagatggc gttccggatg cagatctcgg ttcccgaggt gatgaacatc caggacgagc
     1021 ccccgtatat ccatgagctc tacgggacaa gcccgggcaa gggaacgcta gcgaacaata
     1081 tcctgcttgc ccggaagctg gtcgagaagg gcgtccgatt cgtgcagatc ttcgactggg
     1141 gatgggacag tcacgggacg gatgccagcc tggccataga tgtcggcttc atcaacaagt
     1201 gcaggcaggt ggaccgggcg atgacggcgc tgatcgtcga cctgaaacaa cggggactgc
     1261 tggacgagac cctggttata tggggcggcg aatttgggcg gacgccgatg atggagaaca
     1321 gggaaggcaa gccgaatccc tttaagggca gggatcacca caccgatgcg tttacgatct
     1381 ggatggcggg cggcgggatc aaaaagggcc atgtatatgg cgaaacggat gagatcggct
     1441 acagcgcgat tttgggtaaa gtcgatacct tcgacgttca ggcgaccatt ctcaatcagc
     1501 ttggctttga ccatacgcgc tttacctatg attttcaggg ccggcctttc cggctcaccg
     1561 acgtgaaagg aaacgtcatt aaagagatta ttgcatgatc aaccgaaaac agtttatcgc
     1621 acacagtacc ctggccgcta ccggcctatt gttcgagtcc tttgcccaac cgctttcctt
     1681 cccttccgtc cgcgaaggtt tccagttaaa gatccttgcg acggattggg ggtttcccgg
     1741 ttcgctcgat gaatatgggg ccaaagtccg aaatgccggt tatgacggca tcgagatctg
     1801 gtggtcgctg gatcccggcc ggcaaaagga agtgttcgcc atgcttaaaa aatatggcct
     1861 cgaagtgggc ttcctttgcg gcggctccga gagcgacccg aaggaacatt tcaatcattt
     1921 caagcagatg gtggaaggcg cggcgcgtaa tcccacgcag cggccgctgt atatcaattg
     1981 ccattcgggt cgcgactttt tctcttatga cgaaaacaag ccgttcatcg atctgaccac
     2041 agccctgacc cgcgaaacgg gcatccggat catgcatgag acccatcgtt cgcggatcct
     2101 gtattcggcg ccggttgcca
//
LOCUS       DATGKE010000474         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_27673, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000474 DATGKE010000000
VERSION     DATGKE010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27673"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VKU83_08220"
     CDS             complement(<1..1268)
                     /locus_tag="VKU83_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLI93579.1"
                     /translation="MRSRPTSVINGVTNPLPAAGPSNGHAQPAAASAGASPASAVPGP
                     VSGSADAIASPYSGQLPAATLSAHPGFDSLLQLAARLAGAPCAYILEPDHPTDCHSEY
                     AVNIPLIARTGRHMGHLCLQPETALTDSQAENLKLVADSLVQLLVLAPEKLLTEKLDK
                     QKAFYENILNKLPTDIAVFDSEHRYLFVNPGAISKEEYRKFIIGKDDYEYAAYRKRDI
                     SVAHARRAKFLEAKNSGKEIRWEDSMPNAQGDIITHLRRMYPVYDENGELSLVIGFGI
                     DITDRKLMEEKQAALVKQLSAQNTQLIDFCAIVSHNLRAPLVNIAMLVDCLEETDDTE
                     EHRLLISKLKPVIDNLQLTFNELVESIQIRQDLEIQTEDVCLHECIKRTMTGLQAEIT
                     KLDAQITTDFNAASVIHYAPQYLNSIFHNLV"
     gene            complement(1353..1631)
                     /locus_tag="VKU83_08225"
     CDS             complement(1353..1631)
                     /locus_tag="VKU83_08225"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HLI93580.1"
                     /translation="MKKFLIERNLPGAGNLSAQELQDIARTSCEVVCQLGKPYHWIQS
                     FVTEDKIYCIHIAESEELVREHARLGKFPVNMITEVKTVIDPLTSNLL"
     gene            complement(1729..>4044)
                     /locus_tag="VKU83_08230"
     CDS             complement(1729..>4044)
                     /locus_tag="VKU83_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLI93581.1"
                     /translation="LFNEFLQRFDQEAGPSLIVFEDIHWADEATLDFIKFLARRISRI
                     RCLFILTWRDEEIHGTQPLRQLPGQLPPHSFTRIRLAPLSRKAVEAMAAAKGYRGEDV
                     HGISGGNPFYVQEILASYSPGIPENIKDSILSVYNRFDPETRSVCELLSVVPTGLETG
                     YLEQLEPGYAAALAACLESGIILLKDGRLFFKHELYRRTIEASLSPFTRISLNKRVLT
                     CCVGSFERNGEIERIIHHAKNANDHEAVVQYAPRAAAQAAALGAHVEASKLYYSAIEY
                     YQGKEKDVLVGFYESYAYECHLTNRVREAIIYQQKAANIWKEKGDPERMGNSLSFLSR
                     LWWFDGNRRQAERYAKEAIDTLATRAPSRSKAMAFSNMAQLKMLSDQTVECMHWGEEA
                     IAIAKELEADDILSHAYNNVGSVQMRVPAYRKKGLDLLQLSLDIALRHSFHEHAARAY
                     SNIASNSIQLKDFAFATRILNEGIQYCEEKQLDSWRTYMLSWRARLLLDTGCWSEAAE
                     IAAQLIANESHASIVKIGALVVMGTIRLRTHQPDALAYLMEAKTKAFESMELQRILPA
                     LTALLEYEWITGKAILEKAAIECTIGLLSQGGTLRESSEFDFWFFKTRGQQIPIHPVD
                     TRYLAGDVAQAVQSAAGWEKLGCPYEQALFLFEGDEASKRRAIGIVQRLGAIAVFEKM
                     KCDMRSGGIRSIPRGSRKSTLENPAQLTERELGVLDLLKDGLANKEIGSRLFISAKTV
                     DHHISAILFKLDVGSRTAAVKEAIRRGILTS"
BASE COUNT          808 a         1071 c         1227 g          938 t
ORIGIN      
        1 accaggttat gaaaaatgct gttcaggtat tggggagcgt aatggatgac ggaggccgcg
       61 ttgaagtccg tggtgatctg cgcatccagt ttggtgatct cggcctgcag cccggtcatg
      121 gtccttttta tacattcgtg gaggcagacg tcttcggttt ggatttcgag gtcctgccgg
      181 atctggatgg attcaaccag ctcattgaag gtcagctgca ggttatcgat caccggtttc
      241 agcttcgaga tcagcagccg gtgttcctcg gtgtcgtccg tctcttcaag gcaatcgaca
      301 agcatggcaa tattgacgag cggggcgcgg aggttgtgcg acacaattgc gcagaaatcg
      361 atcagctggg tgttctgggc ggaaagttgc ttcacaagcg cggcctgttt ctcctccatc
      421 agtttacggt cggtgatgtc gatgccgaac ccgatgacga gggagagctc gccgttttcg
      481 tcgtaaacgg ggtacatccg ccgcaggtgc gtgatgatat cgccctgcgc attcggcatg
      541 ctgtcttccc agcggatctc ttttccgcta ttctttgctt ccaggaattt agccctccgg
      601 gcatgcgcca cggaaatgtc tcttttccgg taagcggcat attcatagtc gtccttgcca
      661 ataatgaatt tccggtactc ctccttgctg atcgctccgg gattgacgaa gagataccgg
      721 tgttccgagt cgaagaccgc gatatctgtc gggagtttat tgagaatgtt ttcgtagaac
      781 gctttttgtt tgtccagctt ctccgtaaga agcttctcgg gcgcaagcac gaggagttgg
      841 acaaggctgt cggcgacgag cttcagattt tcggcctggc tgtcggtcaa cgccgtttcc
      901 ggctgcagac acaagtgtcc catgtgtcga ccggtccttg cgatcagcgg gatattgaca
      961 gcatattccg agtggcagtc ggtgggatgg tcgggttcaa ggatgtaggc gcaaggagcg
     1021 ccggcaaggc gagcggccaa ctgaagcaaa ctgtcgaatc cgggatgcgc tgaaagggtt
     1081 gcggccggca gctggccgga gtacggcgac gcgatggcgt ccgcgctgcc tgacacgggg
     1141 ccggggaccg cgctggccgg cgaagcgccg gcgcttgcgg ccgcgggctg tgcgtgcccg
     1201 tttgaagggc cggcagcggg cagagggttc gtcacaccgt ttatgactga ggtcggcctc
     1261 gatctcattt caaggggata gggattttaa tgaaaaaacc taactaacta aaaacgaata
     1321 atttaatcaa ctattgtgga aaacccttca tcttataaaa gattgctggt caatgggtca
     1381 atcaccgtct tcacctcggt gatcatattg accggaaatt tgcccaaacg ggcgtgctcg
     1441 cgcacaagtt cttcgctttc agcgatatgt atgcaataga tcttatcttc cgtgacaaag
     1501 gattggatcc agtggtatgg ctttccaagc tggcagacga cttcgcagga agtgcgggcg
     1561 atatcctgta attcctgggc gctcaggttc ccggctccgg gaaggttgcg ttcgataaga
     1621 aactttttca tttccgttgt ttttttggtg agtgaagaca aagttgcgga taatcgatga
     1681 ctgcgtggtc gggagtcttc cctatttcca ggaaaaaggt tccctatttt acgaggtgag
     1741 gatgccgcgc cggatggctt cctttaccgc tgccgttcgc gatccgacgt ccagcttgaa
     1801 caaaatggcc gagatgtgat ggtcaacggt tttggcggaa atgaagagcc gggacccgat
     1861 ctccttgttg gccaaaccat ctttcaacag gtccaggaca cccagctccc tttccgtcag
     1921 ctgggcgggg ttttcgagcg tggacttgcg gctgccgcgt ggaatgctgc ggattccgcc
     1981 ggatcgcatg tcgcatttca tcttctcgaa gaccgctatc gctcccagcc gctggacaat
     2041 tccgatggcg cggcgcttgg acgcttcgtc gccttcaaaa aggaacaggg cttgctcgta
     2101 gggacagccg agcttttccc agcctgcggc ggactgcacc gcctgagcca catcgccggc
     2161 aagataacgg gtgtcgacgg gatggatcgg gatctgctga ccgcgcgttt tgaaaaacca
     2221 aaagtcgaac tcgctgcttt cgcggagggt cccgccctgg ctaagcaagc caatcgtgca
     2281 ttcaatcgcg gccttttcga gaatcgcttt gcccgtaatc cattcatact ccagcagcgc
     2341 cgtcaaagcc ggcaggatgc gttgcagctc catcgactcg aaagctttag ttttcgcttc
     2401 catcaggtac gcgagggcat cgggctggtg ggtgcgcagc ctgatggttc ccatgacgac
     2461 gagggcgccg atcttcacaa tggacgcatg ggactcgttg gcgatcagct gggcggcgat
     2521 ctcagcggct tcgctccagc acccggtgtc aaggaggagc ctcgcccgcc acgaaagcat
     2581 ataggtccgc cacgaatcaa gctgcttttc ctcgcagtat tggatccctt cattaaggat
     2641 ccgggtggcg aaggcaaaat cctttagctg gatgctgttg ctggctatat tgctgtatgc
     2701 gcgggcggca tgctcgtgga aagaatggcg cagggcgatg tcgagactta gctggaggag
     2761 gtcaagccct tttttccggt aggccggcac gcgcatctgg acactgccta cattgttgta
     2821 ggcgtgcgag aggatgtcgt cggcctccag ctcctttgcg atggcgatcg cctcctcgcc
     2881 ccaatgcatg cactcgaccg tttggtcgga cagcatctta agctgggcca tgttgctgaa
     2941 tgccatggct tttgaccggg aaggagcgcg cgttgcaagc gtgtcgatgg cttccttggc
     3001 gtaacgctcg gcctgccgcc ggtttccgtc gaaccaccaa aggcgcgaca ggaacgaaag
     3061 ggaattgccc atccgctccg ggtcgccttt ttccttccag atgttggcgg ccttttgctg
     3121 gtagatgatg gcctcccgca cccggttcgt gaggtggcac tcgtacgcat acgactcata
     3181 aaaaccgacc aggacatcct tttcctttcc ctggtagtat tcgatagcgg agtagtaaag
     3241 tttcgacgcc tcgacatggg cgccgagagc cgcggcctgg gccgcggccc ggggagcgta
     3301 ctgaacgacg gcttcatggt cgttggcgtt cttcgcgtga tggataatgc gttcgatttc
     3361 gccattgcgc tcaaagctcc cgacgcagca ggtcagcacc cttttattga ggctgatccg
     3421 cgtgaagggc gacagggagg cctcgatcgt gcgccggtag agttcatgtt tgaaaaaaag
     3481 cctgccatcc ttcaggagga tgatgcccga ttccaggcag gccgcgagtg cggcggcgta
     3541 acccggctcg agctgttcga ggtagccggt ctccaagccg gttggcacca cggatagcag
     3601 ctcgcaaacg cttcgggttt cggggtcgaa acggttgtaa accgaaagga tggagtcttt
     3661 tatattttca gggattcccg ggctatagct ggccaggatc tcctggacgt agaaagggtt
     3721 cccaccggaa atgccgtgga catcctcgcc acgatagccc tttgcagccg ccatcgcttc
     3781 gacggccttg cgtgacagcg gtgccagccg gatgcgggta aacgaatgag gcggaagctg
     3841 accgggaagc tgccggagcg gctgcgtgcc gtggatctcc tcgtcgcgcc aggtaagaat
     3901 gaacaggcaa cgaatccggg agatgcgacg ggcgagaaac ttaataaaat ccagggtggc
     3961 ctcgtcggcc cagtggatgt cttcaaagac gatgagggaa ggccccgctt cctggtcgaa
     4021 tctttgcagg aattcattaa agag
//
LOCUS       DATGKE010000475         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1238332, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000475 DATGKE010000000
VERSION     DATGKE010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238332"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="nirK"
                     /locus_tag="VKU83_08235"
     CDS             complement(<1..1173)
                     /gene="nirK"
                     /locus_tag="VKU83_08235"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HLI93582.1"
                     /translation="MGLIFLLILSVTGCKQPDATGNIPNDTAAVIGEYPAELTHAPFV
                     PAGRDNMGPEKVIVNIEVVEKVMKLADGVNYTFWTFGGTVPGPMIRVQEGDLVEMHLS
                     NNPNNKMPHNIDLHAVSGPGGGAEASLTVPGHTSVFSFRAMNSGLFVYHCATAPVGMH
                     IANGMYGLILVTPKGGLPKVDKEFYLMQGEFYTKGKNGEPGMQEFDMDKAIKEQPEYV
                     VFNGAVGSTTGSHALQAKVGDRVRIYVGNGGPNLVSSFHVIGQVFDKVYQEGGSQVHQ
                     NIQTTLIPSGGAAIVEFTCREPGTFNIVDHSIFRAFNQGALAQLVVTGPADKNIYSGK
                     IKDELYQPGNPDASADAGGNAAEPETPELSFADRFKNGNTLFQLNCSSCHQPTGLGV"
     gene            1423..1905
                     /locus_tag="VKU83_08240"
     CDS             1423..1905
                     /locus_tag="VKU83_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLI93583.1"
                     /translation="MKDHAPIKRHQSIASFSRDHHSGLLLVWKIRHGLSKSTDPERIS
                     EYVLFFYKEDLAKHFRDEEKLLFVRLPGDDLLRTQAESDHQAICALMAALEKNPEDVS
                     ILRKIADAVEEHIRFEERELFNHLQDILSPEDLEAIAARLPNDSKVCDEAWKDKFWLN
                     "
     gene            complement(1937..>2392)
                     /locus_tag="VKU83_08245"
     CDS             complement(1937..>2392)
                     /locus_tag="VKU83_08245"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HLI93584.1"
                     /translation="IPFSCLFTYYCLEMFFHLDWTMKYQSALVWSLVGLFTIAISLHP
                     HKMYPVVTGIMTIDILVFLHFIAKVSWLAKATLTYVALLPGFFLVNGVLTGTGLRAPI
                     VNYDPNQIMNVRVLTIPIEDFVYGYALILLNLYFFHVFRRRSPARAIKQ"
BASE COUNT          594 a          614 c          642 g          542 t
ORIGIN      
        1 cacgccgagc ccggtgggtt ggtgacacga ggagcaattc agctggaaga gcgtgttccc
       61 gttcttgaac cggtcggcaa acgacagttc cggggtttcc ggctcggccg cattgccacc
      121 ggcgtcggcg gatgcatcgg ggttgcccgg ctggtatagt tcatccttga tctttccaga
      181 gtagatgttc ttgtccgcag ggccggtaac gaccaactgg gccagggcgc cctggttgaa
      241 tgccctgaat atggaatggt caacgatatt aaaagtgccc ggctcgcggc aggtgaattc
      301 cacgatggcg gcgccgcctg aagggatcag cgtcgtttga atattttggt gtacctggct
      361 gccgccttcc tggtacactt tgtcgaagac ctgtccgatg acatgaaaag aagagaccag
      421 attcgggccg ccattgccga cgtagatcct gacccggtcg cccaccttcg cctgcaaagc
      481 atggctgccg gtggtggagc ctacggctcc gttgaacaca acatattccg gctgttcttt
      541 gatcgccttg tccatatcga attcctgcat cccgggctcg ccgttctttc ccttggtata
      601 gaattctccc tgcatcagat agaactcttt atccaccttt ggcaggccac ctttgggtgt
      661 caccaggatc aggccataca taccgttggc gatatgcata cccaccggcg cggtagcgca
      721 atgataaacg aacaggccgg aattcatggc ccggaagctg aaaaccgagg tatgtcccgg
      781 taccgtcagg ctcgcttccg cacccccgcc gggtccgctc accgcatgca gatcgatatt
      841 gtggggcatc ttattattgg ggttgttgct taaatgcatt tccaccagat ccccttcctg
      901 cacccggatc atcggcccgg gaaccgttcc gccgaaggtc cagaacgtat agttgacacc
      961 gtccgccagc ttcattactt tttcgacgac ttcgatgttg acgatcactt tttccgggcc
     1021 catattgtcc ctcccggcgg ggacaaaggg ggcatgggtc agttctgcag gatactcccc
     1081 gatcacggct gccgtgtcat tggggatatt gccggttgca tccggttgtt tgcagccggt
     1141 gacggatagg atgaggagga aaatcagccc catcaatgat cgtgataaaa gagacggaaa
     1201 taattgccag agaaagaaag cggttttcat cgttgtacct catttaggtc tttagttacg
     1261 agggtcaaaa gtataacagg gccaaatcct cctaaatgac gcctctcaga ataaaacatg
     1321 actaatatca ggtttcactg atcaaaatca gcgttcattt gtcattatat atgatctata
     1381 acgggcctga gcccatcagg aaatgaaaaa acaacacaga taatgaaaga ccatgcaccc
     1441 ataaaaaggc atcaaagcat cgcgtcgttt tcgcgcgacc accattccgg cctcctgctg
     1501 gtttggaaaa tccggcacgg gttgtctaag tccacagatc cggagcggat cagcgaatac
     1561 gtgctgttct tttacaaaga ggaccttgcg aaacatttca gggacgaaga gaaactcctg
     1621 tttgtcaggt taccgggcga tgacctctta agaacacaag cggagtctga tcatcaggca
     1681 atctgcgcgc ttatggccgc gctggagaaa aatcccgagg acgtctctat tctccggaaa
     1741 attgcggatg cagtggagga gcatatccgt ttcgaggaac gcgaattatt caaccacctg
     1801 caggatatcc tttctccgga ggacctggag gctatcgctg cacggctccc caatgacagc
     1861 aaggtctgcg acgaggcgtg gaaagataaa ttctggctaa attgatgtcg atccaccgga
     1921 tgacggagcg accgaattat tgttttattg cccgggcggg actccgccgc cggaagacgt
     1981 ggaaaaaata tagattgagc aggatcagcg cgtatccgta tacgaaatcc tcgatcggga
     2041 tggtcaatac acgcacattc atgatttgat tcggatcata gtttacgatg ggagcgcgga
     2101 ggccggtgcc ggtcaggacg ccattcacga gaaaaaaacc cggcaagagg gcaacatagg
     2161 tcagcgtcgc cttcgccagc caggacactt tcgcgataaa atgaagaaag accagtatat
     2221 caatggtcat tattccggta accaccggat acattttatg cgggtggagc gaaatggcta
     2281 tcgtgaagag gcctactaag gaccagacga gcgcagactg gtatttcatg gtccagtcaa
     2341 gatggaaaaa catctccaga cagtaatagg taaagaggca cgagaaggga at
//
LOCUS       DATGKE010000476         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1264456, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000476 DATGKE010000000
VERSION     DATGKE010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264456"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VKU83_08250"
     CDS             <1..1172
                     /locus_tag="VKU83_08250"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HLI93585.1"
                     /translation="IIKGSIDEDLFLTRYEPQDPNRRYQLSFAAEFLGPSFFITVKKK
                     HEIGLTIALRGMGNIRDITGNAGQNAFDYLLGSSLWNTTYQEQSARINGMGWMEWGLH
                     YATVLFADDRNELKAGATLKYEQGMFAAYVKNTNLTYRIADTTGLIFTHSAVDYGRTD
                     FGDFRRRNFHNFDHGHGLGVDLGFTFVHKVNPGDDRYRYRIGFSLLDVGKINYDRNSG
                     SYHLSAVSANFSNWHETQFVDNAQLDRTLSAVFYNGDSSRSMTGNSFHMALPAALSVQ
                     ADWDVAGPVFAGLTIVQGFGHGKNVGVTRPDVYSVAPRYETRWWEVSVPLSLIYYGHW
                     QPRVGIAVRAGYFFIGGDAPGALLKLNDLGQVDFYAGLHYFMPSKSKQPPLFTHL"
     gene            1183..1722
                     /locus_tag="VKU83_08255"
     CDS             1183..1722
                     /locus_tag="VKU83_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLI93586.1"
                     /translation="MKASALFGGIAIVVAGIFVATNWTVDPANAKVAFSVGGPFGTVH
                     GSFTGLQATIRFDPADLPGSSIEASIDASTVSSGIGLRNHHLRSEEQWLNTDKYPRIS
                     FRSARIERAASGYKVKGNLTLKNVTRPIEIPFTFAPNGNTGVFKGQFSFKRLDFDLGK
                     PGGTVGEVITIDLDIPVKK"
     gene            1766..2668
                     /locus_tag="VKU83_08260"
     CDS             1766..2668
                     /locus_tag="VKU83_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLI93587.1"
                     /translation="MERTDICCIGHITLDKVVTPRDTVYMAGGTAFYFSNAVSRLDVS
                     YRLITALAADGMPAVEKLRAAGVAVTVLPSAHTVYFENIYGDDQDHRKQRVLQTADPF
                     AERQLADAQARIFHLGPLLAGDMPVGLIRMLAGKGRISVDAQGYLREVVAQEVRAVDW
                     LEKKAALQYIDILKSNEAEMTVLTGQTGIRQGARMLADWGVKEVVITLGSQGSVIYAD
                     QRFHDIPAYVPNTTVVDATGCGDTYMAGYLYQRSKGMPVQGAGEFGAAMATLKIEASG
                     PFTGTREQVQALLASRRATAFVCR"
BASE COUNT          617 a          738 c          825 g          582 t
ORIGIN      
        1 ggataatcaa gggcagcatt gacgaggacc ttttcctcac ccggtatgaa cctcaggacc
       61 ctaacaggcg gtatcagttg agttttgcgg ccgaattttt gggcccttcg ttcttcatca
      121 ctgtcaaaaa aaagcacgaa atcggcctga caattgccct ccgcggaatg ggaaatatcc
      181 gggacatcac gggtaacgcc ggacaaaatg ccttcgacta cctgctcggc tccagtctct
      241 ggaatactac ttatcaggaa cagtcggcgc ggatcaacgg gatgggttgg atggaatggg
      301 ggctccatta tgcaacggtg ctgtttgcgg atgacaggaa cgagctcaag gccggggcta
      361 cgctaaaata tgagcagggc atgttcgcgg cctatgttaa gaatacgaac ctgacgtatc
      421 gcattgcaga cacgacaggg ctaattttca cccattccgc ggtcgattac gggcggaccg
      481 atttcggtga cttccggcgc cgcaactttc ataattttga tcatggtcat ggcctcggag
      541 tggacctggg atttaccttc gttcacaagg taaatccggg agatgaccgt tatcggtacc
      601 gtatcggatt ctcgctgttg gacgttggaa agataaatta tgacagaaac tcgggttctt
      661 atcacctttc ggcggtgtcg gccaattttt ccaactggca cgaaacgcag ttcgtcgata
      721 atgcccagct ggaccggacg ctcagtgccg tattctataa cggggactca tccagatcga
      781 tgacgggcaa ttccttccat atggccttgc cggcggccct gagcgtacag gcggattggg
      841 atgtcgcggg ccctgttttc gccggcctca cgatcgtgca ggggttcggc catggaaaga
      901 atgtcggggt gacgcgcccg gatgtttatt cggtggcgcc gcggtacgaa accagatggt
      961 gggaagttag tgttcccttg tccctgattt actatggcca ctggcagccg cgggtgggca
     1021 tcgcggttcg ggcaggctat ttcttcattg gcggcgatgc accgggagca ctgttgaagc
     1081 tgaacgacct cgggcaggtt gacttttacg caggcctgca ttattttatg ccctcgaaat
     1141 caaaacaacc acctctattt actcaccttt aaagacagca ggatgaaagc aagcgcgttg
     1201 tttgggggaa tcgcaatcgt tgtggcggga atatttgtgg cgaccaattg gacggtcgac
     1261 cctgcaaacg caaaagttgc tttcagtgtc ggcgggccat tcgggaccgt gcacggcagc
     1321 ttcacggggc tccaggcgac cattcgtttc gacccggcgg atctgcccgg cagctccatc
     1381 gaagccagca tcgacgccag cacggtgagc agcggcatcg gccttcgcaa ccaccacctg
     1441 cgtagcgaag agcaatggct gaataccgat aaatatccca ggatctcgtt caggtcggca
     1501 aggatcgaaa gggcggcctc cgggtacaaa gtaaagggca accttaccct caagaatgtc
     1561 accaggccca tcgagatccc ctttacgttt gccccgaatg gaaatacggg cgtattcaag
     1621 ggccagttca gtttcaagag attggatttt gacctcggaa aaccaggcgg aacggtcggc
     1681 gaggtcatca ccatcgacct ggatatcccc gtaaaaaagt aagggtggct ggcgaaatag
     1741 ccctaccttt agggtttgta tcgatatgga aaggactgat atctgctgca ttggacatat
     1801 taccctggac aaggtggtca ctccgcgaga caccgtctac atggcgggcg gcacggcttt
     1861 ttacttttcg aacgcggtgt cgcggctcga tgtaagctac cggcttatca ctgccctggc
     1921 ggcagatgga atgcccgcgg tggaaaagct ccgggcggca ggcgtggcgg ttacggtcct
     1981 accgagcgcg cacacggttt atttcgaaaa tatttatggc gacgaccagg accaccggaa
     2041 acagcgcgta ctgcaaacgg cggacccctt cgcggagagg cagctggcgg atgcccaggc
     2101 gcggattttt cacctcggcc cgctgctcgc cggcgacatg ccggtcgggc tcatcaggat
     2161 gctggcagga aaaggcagga tctctgtcga cgcgcaggga tacctgcggg aagtcgtcgc
     2221 ccaagaggtg cgtgcggtcg actggcttga aaaaaaagcc gcgcttcaat atatcgatat
     2281 cctcaagtcg aacgaggcgg agatgacggt gctgacgggc cagaccggaa tccggcaagg
     2341 cgccaggatg ctggcagact ggggggtgaa ggaggtcgtc atcacgctgg gcagccaggg
     2401 gtcggtgatc tatgcagacc agcgttttca tgatattcct gcgtatgtac cgaacacgac
     2461 cgtcgtcgat gccaccggct gcggcgacac ctacatggcg ggctaccttt atcagcgtag
     2521 caagggaatg cccgtgcaag gggcgggaga attcggcgcc gccatggcta cgctgaagat
     2581 cgaagcgtcg ggcccgttca ccggtacgcg cgaacaggtg caagcgctgc ttgccagccg
     2641 ccgggctacc gcgtttgtct gtcgctaaac cacatgaaac catggaagcc tccagtccaa
     2701 ccgaagttct ccagatcatg ctaaaaaagg accggttcag ccgctggctc ggcctggcga
     2761 tc
//
LOCUS       DATGKE010000477         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_301314, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000477 DATGKE010000000
VERSION     DATGKE010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301314"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..973
                     /locus_tag="VKU83_08265"
     CDS             89..973
                     /locus_tag="VKU83_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HLI93588.1"
                     /translation="MHGLLLLAFLACKKTPPAKIELTPLQSLVNTDSTLTLYHRMILR
                     GNDAGLLNDAPATLFFPRNAVLFQSGYTALIIDSMSSALADRIVRYNYLPAAINADTA
                     VYTANPTLLGIPLYVLEGSNGQLLLNGSIPAVNPPAKVGQASVYYLDSLVPPAADSLP
                     LLLQNDSTLTYMNEVLFRTNLYDSLLLTGSYTLLAPTNTAFQQAGWDSLGAIDSANVD
                     TLLNLAMNQVVKGAYFSNTFPATVMTLRGAGITVTTVNGLLRFAGAGNSVPVNWLSGN
                     QVAGPTLILHHTDGILKP"
     gene            1057..>2001
                     /locus_tag="VKU83_08270"
     CDS             1057..>2001
                     /locus_tag="VKU83_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLI93589.1"
                     /translation="MRTFHDIKNMLQHNKIADFVKTNKLTPRDIAEFANRHTQGAVKL
                     FQQLDPKHAVRAFKFMRHKKQEAILKSLPEEKAAELLNAMQPDDRTAFLEELPGNYVK
                     ELLKILTPENRAETLELLGYAEGSVGRLMTPDYVAIDENETCQEVLTVIRHRGQATET
                     LNWLFVVDNKGVLIDDINIKDFLIVDPQTRVAEIMDHQFLALNVNDNQKDVINIFRNN
                     SRFALPVTDNNGVLLGIVTLDDVLRLAESEGTREIQKIGGSEALDEPYSTISFTRLIR
                     KRAGWLIILFLGEMLTATAMGHFEDEISKAVVLALFVPL"
BASE COUNT          455 a          594 c          521 g          431 t
ORIGIN      
        1 aaggactggg aaaagatcat gacccgcgac cggcttcgag ctttatatga aaagctgtaa
       61 gacgatggaa aggcgcttaa ggtatactat gcacggtctg ttgctgctgg cattcctggc
      121 ctgtaaaaag acgccgcccg ccaagatcga gctcaccccc ctgcaatccc tcgtcaacac
      181 cgatagcacg cttaccctat atcatagaat gatcctgcgg ggcaatgatg ccgggctgct
      241 caacgatgcg ccggccacgc tgttcttccc caggaatgcg gttctgttcc aatccggcta
      301 tactgcgctc atcatcgatt cgatgagctc tgcgctcgcc gatcggatcg tacgatataa
      361 ttatcttccc gccgctatca acgcggatac ggcggtttat accgccaacc ctaccctcct
      421 tgggattccg ctttatgtcc ttgagggcag caatggccag cttttgctca acggaagcat
      481 tcctgccgtt aacccgccgg ccaaggttgg ccaggcctcc gtgtattatc tggattccct
      541 ggttccgccg gcggccgact cgcttccgct cttgctgcag aacgatagta cactcacgta
      601 catgaacgaa gtcctcttcc gcaccaatct ctacgattcc ctgctgctca ccggtagcta
      661 tacgctgctg gcgcctacca ataccgcctt ccagcaggca ggctgggaca gcctcggcgc
      721 catagattcc gccaatgtcg ataccctgct caatctggcg atgaaccagg tggtgaaggg
      781 ggcctatttt agcaacacct tccccgcaac cgtgatgacg ctccggggcg ccggtatcac
      841 cgtcactacg gtaaatggcc tgctgcgctt cgcgggcgca ggtaattctg tgcccgtgaa
      901 ctggctgagc ggcaaccagg tggcgggacc taccctgatc cttcaccata ccgatggcat
      961 cctgaagcca tagaagccac aatggctcca attgtaacat taaaataatg tatgcctgtt
     1021 ttcccggaac agagccgggt tcgttatttt tgatgcatgc gcactttcca cgatatcaaa
     1081 aatatgcttc agcacaataa gatcgcggac tttgtcaaga ccaacaagct gacgccgcgg
     1141 gacattgcag aattcgccaa ccgtcatacc cagggagcag tgaaactctt ccagcagctg
     1201 gaccccaaac atgccgtccg ggcctttaag ttcatgcggc acaaaaaaca ggaagccatt
     1261 ctcaaatccc tgccggagga aaaggctgca gagctgctca atgccatgca acccgatgat
     1321 cgcacggcct ttctggaaga gctgccgggc aactacgtca aagagctgct aaagatcctg
     1381 accccggaga accgggccga aaccctcgag ctgcttggtt atgcggaagg ctccgtcggc
     1441 cgtctcatga cccccgatta tgtagcgatc gatgagaatg agacctgcca ggaggtgcta
     1501 acggtcatcc ggcatcgcgg ccaggcgacg gagaccctca actggctgtt cgtcgtcgac
     1561 aacaaaggcg tgctgatcga tgatatcaac atcaaggact ttctgatcgt cgacccgcag
     1621 accagagtag ctgagatcat ggaccaccag ttcctggcct tgaatgtgaa cgacaaccag
     1681 aaagacgtca tcaatatctt ccgcaacaat agccggttcg ccctgccggt gacggataac
     1741 aacggggtgc tgctggggat cgtgacactg gacgacgtcc tgcggctggc ggaatcggaa
     1801 ggtacgcgcg agatccagaa gatcggtggt tccgaagccc tggacgagcc ctattcgacg
     1861 atctctttca cccggctcat ccgtaaacgt gccggctggc tgatcatcct ctttttggga
     1921 gagatgttaa cggctacggc tatggggcac ttcgaggacg agatcagcaa ggctgttgtt
     1981 ttggcccttt tcgtcccgct c
//
LOCUS       DATGKE010000478         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1095547, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000478 DATGKE010000000
VERSION     DATGKE010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095547"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VKU83_08275"
     CDS             <1..1243
                     /locus_tag="VKU83_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HLI93590.1"
                     /translation="RPAWLWASIEAIPWTYDKVVVQAEFANISWKTVGYNLQLCHWSW
                     DDDGFVTNQLAHPAHGSLFFNSFRSNGYNFWQSTAASFAGSYVWETFSEKQAPAPNDF
                     INTGFGGMVLGEMINRFSNKIIDNGRRGIGRQVSEIFAFIINPTKGLSRIIDGKWGKV
                     LPNSPQRDSSWVELEVDAGIREFRVDRGNWKSGLYGHLKIQYGSPYENYQTPFSHIYL
                     SAEFGEDDSSKVNVVSAYGSLRGWRIAETENSRHLVMLTANYDFIDNEAFFYSAQSVK
                     VNLFSEYGLTHKIKFSTVVGAGSILLAAVPNQYSYAGRNYDYCTGVGFNGSGVVNINE
                     HLYLTVAYRGGWFTTLSGTATDYFLHTVSGEISYRIMDGLSICAEPAYFRLHGNYGHD
                     PDIDKTYPYLRISLRYGLRTH"
     gene            1253..2260
                     /locus_tag="VKU83_08280"
     CDS             1253..2260
                     /locus_tag="VKU83_08280"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HLI93591.1"
                     /translation="MNWTRPAFGKRATLVAGVLAIWMHLDSAAQDTLRSQHFFSIRID
                     DDFLNLAAQGTDRYYTGGDYFRYSFLAVAGKKTVLRKVLYAPFSNVASLYSVGATQWV
                     YTPDILSAKIPVKGDYPYCGVLFLRFTREMVSRDAKSLFRSGLSLGMMGPAALGKEAQ
                     SAIHGWINTVRPAGWKYQLPDYPVVNYSLYYESNLFSISRRFKINATGSVEAGTLQTD
                     AQLGLDGLILDRNDNFFPGRGVRTGNLENRRPKFYIQLKPAVKFVGYNGILEGGLFDS
                     RQFYHISPGQMSRVLFEGAGIMGMQLGDFSIQYRQAIEGAEFRTVEMHVYGGFLLQWK
                     I"
     gene            2523..>2770
                     /locus_tag="VKU83_08285"
     CDS             2523..>2770
                     /locus_tag="VKU83_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93592.1"
                     /translation="MEDDARPNYVVFDNGGRTPDRYTIVDRESGNVFAAGEPIGEAEI
                     SVKYCGNCADHRIVLYGAGWRQLPLSRRIISAETDNYVR"
BASE COUNT          663 a          638 c          774 g          695 t
ORIGIN      
        1 ccgacccgca tggttatggg cgtcgatcga agccatcccg tggacatacg acaaagtggt
       61 tgtgcaggcg gaatttgcga atatctcctg gaagacggtc ggctataacc tccaactctg
      121 ccattggagt tgggacgacg acggttttgt gaccaaccaa ctggctcatc ccgcgcacgg
      181 gagtcttttc ttcaattctt tccggtccaa cgggtataat ttctggcaat cgacagcagc
      241 ctcctttgcg gggagctatg tctgggagac tttttcggag aagcaggccc ccgcgccaaa
      301 cgatttcatc aataccggtt ttggcgggat ggtgctgggg gaaatgatca accggttctc
      361 gaataagatc atcgacaatg gccgccgggg aatcggtcgg caggtcagcg agatttttgc
      421 cttcatcatc aatccaacaa aagggttaag caggataatc gatggcaaat gggggaaagt
      481 ccttcctaat tcaccgcagc gcgactcgtc ttgggtggag ttggaagttg atgccgggat
      541 aagagagttc agagtagatc gcgggaactg gaaatcgggt ttgtacggcc atttaaagat
      601 ccagtatggc agtccctatg aaaattacca gacgcctttc agccatattt acctcagtgc
      661 ggagtttggg gaagacgaca gctcaaaggt gaatgtggtc agtgcgtacg gatctctccg
      721 gggctggcgg atcgctgaaa cggagaactc ccggcacctg gtcatgctta ccgccaatta
      781 tgattttatc gataacgagg ccttttttta cagtgcgcag agtgtcaagg tcaatttgtt
      841 ctctgaatat gggcttaccc ataaaataaa gttcagtacg gttgttggcg caggttctat
      901 cttgctggct gccgttccga atcagtattc gtacgccgga agaaattacg actattgcac
      961 gggcgtcggc ttcaacggca gcggagtcgt taacataaat gagcatttat atttaaccgt
     1021 cgcctacagg ggcggttggt tcaccaccct cagcgggacg gcgaccgact attttttaca
     1081 tacggtttcg ggcgagataa gttaccggat catggacggg ctttccatct gtgcggagcc
     1141 ggcatatttc aggttgcatg gcaattacgg gcatgatccg gatatagata aaacctatcc
     1201 ttatttgcgt atttcgctcc ggtatggcct gcgcactcat taaaaaaaga acatgaactg
     1261 gacaaggccg gcgtttggta aacgggcgac cctggttgcc ggcgtactgg caatctggat
     1321 gcacctggat agtgcggctc aggataccct tcggtcacaa catttcttct ccattcggat
     1381 cgatgatgac tttctgaacc tggccgcaca gggaacggac cgttattata ccggtggtga
     1441 ttactttcgt tattccttcc tggccgtcgc gggcaaaaag actgtgctcc ggaaagttct
     1501 atacgcacct ttttccaatg tcgcctcgct ttattccgtc ggagccacgc aatgggtcta
     1561 tacccccgac attttgtccg cgaagatacc ggtgaaaggg gattaccctt attgcggggt
     1621 tttgttcctg cgctttacca gggaaatggt atcgcgggat gcaaaaagcc tgttccgctc
     1681 tggcctgtca ttggggatga tgggtccggc cgccctgggg aaagaagcgc aatcggcgat
     1741 acatgggtgg attaataccg ttcgaccggc aggttggaaa tatcagttgc ccgattaccc
     1801 ggtagtcaat tacagcctct attatgagtc caatcttttt tcaatcagcc ggcgctttaa
     1861 gataaatgca acggggtccg tggaggcagg caccttacaa acagatgcgc aactgggatt
     1921 ggacgggctg attttggatc gaaacgataa tttctttccc ggcaggggcg tcaggacggg
     1981 caatcttgaa aacagaaggc caaaatttta cattcagcta aaaccagcgg tcaaatttgt
     2041 aggatataac ggcattttgg aaggcggcct ctttgacagc cggcaatttt atcatatttc
     2101 accgggtcag atgagcaggg tcttgttcga gggcgccgga atcatgggca tgcagctagg
     2161 cgatttttct atccagtacc ggcaagcaat cgaaggcgcg gaattcagga cggttgaaat
     2221 gcatgtgtat ggcggctttc tgcttcagtg gaagatttga ataaggaaga gcagggcgcg
     2281 tcgcggattg ggcgcgtcgc ggattgccca acattcgcat ggtcgggaag gagacgatga
     2341 ctatatattc cattgcattc tttgtaagcg ctttccctcc cgggcagcga ggagcagcca
     2401 ttccgaaatg gaagggtatt caagaatgga aaggcatggg tacggcggat agtgccgatt
     2461 atcaatcaat tatgtaatag gaatgggttt tggtttcgtt catcagccat aaaacaataa
     2521 aaatggaaga tgacgcaagg ccaaattatg tggtttttga caatggtggc cgcactcccg
     2581 accgttatac catcgtcgac cgtgaatcgg gcaatgtttt cgccgctggg gaacctatcg
     2641 gcgaagctga gatttctgtc aagtattgcg gcaattgcgc cgaccaccgg atcgtcttat
     2701 acggagccgg ctggcgacag ctgccgttga gccgtaggat cataagtgcg gaaacggata
     2761 attatgttcg
//
LOCUS       DATGKE010000479         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1129102, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000479 DATGKE010000000
VERSION     DATGKE010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129102"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VKU83_08290"
     CDS             <1..273
                     /locus_tag="VKU83_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93593.1"
                     /translation="QTKSKSDEDMLNYPIRINDKLSGLYGIAAGGYAVPTRQAREVFA
                     ELSARADVQLDRLKSIINDELAALNKLIYERQVPVIGTKPEDEGEK"
     gene            270..>2161
                     /locus_tag="VKU83_08295"
     CDS             270..>2161
                     /locus_tag="VKU83_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLI93594.1"
                     /translation="MIESNVLDLSSTLGLIATAVLSFNFLLGILLSTAYRRSPLWKRM
                     PAWVRRVSLDDLHNWTAYVALALAFAHPVVLLADKALKYRVADIVLPVSAPHQPVWTV
                     LGSLALYALIVVIVTTQKAVKRRLGFRTWKNIHLVSYGTALLFVIHGLVMDPELKDRP
                     IDWLDGEKLLSEICGLLLIAAAFIRWRHYRRATSQKSAVGKVLMVLLAATVLSGSLHA
                     QNAVTGRITDALSGSPLSGVSVYIPDLKSGAVSGQDGSYRIRDLPSGVYVISITMIGF
                     DRLVDEIAVKGTIHKDYRLRPATIPLRDVVVTGVPVATDKQNSPYIITTVDYSRLLEA
                     SSTNVIDAISKEPGVSAMTDGQSISKPVIRGLGYNRVLTINDGVEQVDQTWFDEFGIE
                     ADPDAVHHYEILKGPASLAYGSDAIAGVVNLIPADPLPEGQTKGDILVNYQTNNGLIN
                     AMGHVAGTRNGISWSARIDNIMAHSYRDPYDGYVLNSQFSDFNTDATISLHRGWGYMQ
                     LHASYFDMATGIVDGTRDSATGIMEREVAYPGLNGGEPTYVIPTHQEQTSYTPFVINQ
                     RIRHSKLVWDNHFALGTGYISAIFSGQKNQRQETNDPALPDSPDIYYYSTGGTYDVRY
                     VSPQFAG"
BASE COUNT          494 a          574 c          618 g          475 t
ORIGIN      
        1 cagacaaaat caaagagcga tgaggatatg ctcaactacc cgatccggat caacgacaag
       61 ctgtcgggtc tgtatggcat cgcagcgggc ggctatgcgg tgccgaccag gcaggcgcgg
      121 gaagtttttg ctgagttgag cgccagggcc gacgtgcagc tggaccggct gaaatcgatt
      181 atcaacgacg agctggcggc gttgaacaag ctgatctatg aacggcaggt gccggtgatc
      241 ggtacaaaac cagaagatga gggggaaaaa tgatcgaatc caacgtgctt gacctttctt
      301 ccacgctggg acttattgcc acggcggtgc tgagctttaa ttttttgtta gggatcctct
      361 tgagcaccgc ttatcgccgg tcgccgctgt ggaagcgaat gccggcatgg gtcaggcggg
      421 ttagcctcga tgacctccat aactggactg cctatgtggc ccttgcgctg gcattcgccc
      481 atccggtggt tttgctggca gacaaggccc tgaaataccg ggtagccgat atcgtgcttc
      541 cggtgtcggc tccgcatcag ccggtatgga cggtgctggg gtcgctggcg ttgtatgcgt
      601 tgatcgtggt gatcgttacg acccagaagg cggtgaaaag gagactggga ttccgcacct
      661 ggaaaaatat ccacctggtc tcgtacggca ccgcattgct tttcgtcatc catggtttag
      721 tgatggaccc cgagctcaag gaccggccga tcgactggct ggacggagaa aaactattgt
      781 cggaaatctg cggactgtta ttgatcgccg cggctttcat ccggtggcgg cattaccggc
      841 gggccacaag ccaaaagtct gccgtcggca aggtcttaat ggtattgctg gcggcgaccg
      901 tcctttctgg aagtctgcat gcacagaacg ccgtcacggg aagaataacg gatgccctaa
      961 gcggttcccc actgtccggc gtatccgttt atattccgga cctgaagtcg ggagccgttt
     1021 ccggacaaga cggaagctac cgcatcaggg acctcccttc cggcgtttat gtcatttcca
     1081 tcaccatgat cgggtttgac cggctggtag atgaaatcgc cgtgaaagga acgatccata
     1141 aggactaccg gcttcgcccc gctacgatcc cgcttagaga tgtggtggtc acgggtgtgc
     1201 cggtcgcaac ggataaacaa aattctccct acattattac cacggtggac tacagccgct
     1261 tattggaagc cagttccacg aacgtcatag atgccatcag caaggaaccc ggggtatctg
     1321 ccatgacgga cggccaaagc atttcaaaac ctgtcatccg cggcctgggt tataaccggg
     1381 tcttgacgat caacgacggg gtagagcagg tcgatcagac ctggtttgat gaattcggga
     1441 ttgaagccga tcccgacgcc gtgcaccatt acgaaatctt gaaagggccg gcgtcgctag
     1501 catacggatc cgatgcgatc gccggggtcg tcaacctcat ccccgcagat ccgttgccgg
     1561 aaggacaaac gaagggcgac atcttggtta attatcaaac caataacggc ctgatcaatg
     1621 ccatgggaca cgtggcgggt accaggaacg gcatttcctg gagcgcgcgg attgacaata
     1681 tcatggcaca ttcttaccgg gatccctatg atggatatgt actaaactcc caattcagcg
     1741 atttcaatac agatgctacc attagccttc accgtgggtg ggggtatatg caacttcacg
     1801 ccagttattt cgacatggcg acgggaattg tcgatgggac cagggactcg gctacgggaa
     1861 ttatggaaag agaggtcgcc tatccgggtc ttaacggcgg agaacctacc tacgtcatcc
     1921 ctacccatca ggagcaaacc tcttatacgc catttgtcat caaccaacgg atccggcatt
     1981 caaagctcgt ctgggataac catttcgctc tgggcaccgg ctatatcagt gcgatttttt
     2041 ccggccagaa gaaccagcgg caggaaacca atgatccggc gctgccggac tcgccggata
     2101 tttactatta ttcgaccggc ggcacgtatg acgtccggta cgtgtcgcct caattcgcgg
     2161 g
//
LOCUS       DATGKE010000480         5797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_10044, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000480 DATGKE010000000
VERSION     DATGKE010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10044"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VKU83_08300"
     CDS             complement(<1..204)
                     /locus_tag="VKU83_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93595.1"
                     /translation="MKTTIAIRAENISKTFHIYEDSHNTVKHRLFNLFNPPRARRVEA
                     LKPMSFEIKKGECIGLIGRNGCGK"
     gene            complement(206..1000)
                     /locus_tag="VKU83_08305"
     CDS             complement(206..1000)
                     /locus_tag="VKU83_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93596.1"
                     /translation="MAYTTARGMMNPHKLERLWLLARIEFKLRYYENKLGLLWALLKP
                     VMDIVIYYVVFQIIIRQNIPAFASYLFIGLILWNFFVESTTGTIQILFTKKYLYEYSN
                     MNKMEIYVSTLVSNSIGFFFNFVMFLLFYNFIEKESLGPSVENFWLLPIFCILFVLSL
                     GSSLILSNIYILAKDISQIWQVAVSFLFFLSPIFYKLSTFKHSLPRFDYGNPMAGIII
                     NARRVMMEHRPPDMRLLAWDMLYALTVLGAGLLLLQKLGTKAAEKL"
     gene            complement(973..3168)
                     /locus_tag="VKU83_08310"
     CDS             complement(973..3168)
                     /locus_tag="VKU83_08310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93597.1"
                     /translation="MKYWLLTTEFPPAHGGGISTYCRCTAEMLAGKGVAVTVIVADDS
                     VDDYSLTSFQRHLVIARFNPARRGVPRELGGAAGLSYAFLRMAQLMVRELGEPDIIES
                     QDYLGIAYYIQQFQLLGYPSFKDIPVLITIHAPAFVYLPYNGTSVYRFPDFWTCEMEK
                     HSIRSADMVISPSRFMAAEVGRHLDYGQRPIVLPYAYRPCDPHSAAGDRDRAVRRNKI
                     VYYGKLSPQKGSFELLAYFKALWDDGFPHPLHIIGGDDIVYQPELLTMGQLVHRRYGS
                     YIRKKLLCLHGKIDPAAIPTALADAHVVLVPSVVDNLPFVCLETMACGKVVLASVQGG
                     QSEIITDGVNGFLFDHREPESFGRKLKQVLALSDEEILRIGRTARASLDRYDYDLIAP
                     KKIALLKELRKTYSRRKLFPFLRPGPAPAPLPEKPFISNMLSVVIPYYNLGAYLDECV
                     LSILNSRWERTQIIIVNDGSSEPGSLAALERWRKHPAITVIDTPNRGLAQARNAGAAQ
                     AAGAYLSFLDADDAVRPDYYEKAIRVLDQYKDVYFVGSWVEYFGDSAGVWPAFPPERP
                     YLLAHNPINSSALVYKTAAFLQAGLNDGQLVFGLEDYESVVRMARHSFNGVVLPEPLH
                     RYRVRQGSMIRAMNRNKLLLAHTYITEKHREFYQPYMAEVIQLLNSNGPGYLFENPTL
                     QLYVNSSRDRWNWVNAVKRIARRHPALKKFILLLKKKTDWIWHTPPHAA"
     gene            complement(3165..4016)
                     /locus_tag="VKU83_08315"
     CDS             complement(3165..4016)
                     /locus_tag="VKU83_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93598.1"
                     /translation="MLNFFYYLNHAPETAETAGRMASAAGIPVGHVVHSGSTDTLPLL
                     RHQYSSRGNYPFCLALEGACGEEVVGAARRLLLLFFHPFYEYAEWAKPLFVYVPGRER
                     AAARAGLTELFNQQRLEAALYFPGEQSTDGGVRPAIYTYSAATAGVADRYYRYCLTSY
                     SPRDIFLLEDAPDTFALIQQADAQLRAEHPLLYRLADDSRQARRCLTRLSREKLQLAE
                     ESRIRQQLLEAASKQNEVEYILRFYRNEYEILPLWYKRLGHIIKVLQGKRSFRSLYDK
                     SVKKYKE"
     gene            complement(4016..4729)
                     /locus_tag="VKU83_08320"
     CDS             complement(4016..4729)
                     /locus_tag="VKU83_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HLI93599.1"
                     /translation="MERYRYSILYHNYYDEAGIDELRSKIEALDDPGILLLCSLPDKF
                     AGLRPPQGDSEKFVVAANVGKDIGGKLILVELLLRLYPDTPYAIFLHDKRSYQKHSGR
                     WEKDGLFSIVSPEKFRRIGAAFDRDAGMGIACAAGHVRNEYLGSGNFATKNSELLTGM
                     MKKYSIGDADLRFVAGTMFWIRTSLLRDFFSREDALAVRATLEAGNVLDQEQGTMTHC
                     WERMLSWIALSAGCKLKEF"
     gene            complement(4729..>5797)
                     /locus_tag="VKU83_08325"
     CDS             complement(4729..>5797)
                     /locus_tag="VKU83_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93600.1"
                     /translation="ALLENCRTHFSSEQECCLGYLREAGLFDPGKKAVVDIGYSGTLQ
                     RILNDVTGRAGGNVEPVHGYYMVLYDTFDTLVRNPAVRATGLFGDRIDPKLKKLSIDK
                     YSLFYEMILSSVRGPVCCYAQKPDGAHRPVYAPVSTDEKYKLMKLPIVHEGILHYCRD
                     LMELTDVAAITADDADFLLTPFQTFLEAPTQADLDMLAGYSLDDDYCGQKILYWAPPP
                     GAGALSQADFLWKRYVPAAPLWFEKPAPAIRPAFGGFATRKEYEIFTWYREQYESLPG
                     WYKAVGQLLKVIRGTKRIRLLLEDVNYVPAAGTKADEIQGWYDKEYEVLPRWYKSFGQ
                     LIRILMGKRGWSGAKNKTIAT"
BASE COUNT         1279 a         1632 c         1635 g         1251 t
ORIGIN      
        1 ctttccgcaa ccgttgcggc cgatgagccc gatgcattcg ccttttttta tttcaaagct
       61 catgggcttc agcgcttcga cgcgcctggc gcgcggcggg ttgaaaaggt taaagagccg
      121 gtgtttgacg gtattatgac tgtcttcgta aatatggaag gtcttgctga tattctctgc
      181 ccggatggcg atagtcgttt tcatgtcaca gtttttccgc ggccttagtg cctaattttt
      241 gcaaaagcag gaggcccgca cccaatactg tcaacgcata gagcatatcc catgccaata
      301 gccgcatatc cggcggccgg tgctccatca ttacgcgtcg cgcattaatg atgatccccg
      361 ccatcgggtt tccatagtcg aaccggggca ggctatgctt aaaggtgcta agcttgtaaa
      421 agatcggtga caggaaaaac aggaaggaaa ccgcgacctg ccagatctgg ctaatgtctt
      481 ttgccaggat ataaatattc gacaggatca gcgaagagcc tagggacagg acaaaaagga
      541 tgcaaaagat cggcagcagc caaaaatttt cgacggacgg ccccagtgat tccttttcaa
      601 taaaattgta aaacagcagg aacatcacaa agttaaagaa gaagccaatg ctgttgctca
      661 ccagcgtgga gacatagatc tccattttgt tcatgttgct gtattcatac aggtattttt
      721 ttgtaaacag gatctggatg gtgccggtgg ttgattcgac gaagaagttc cagaggatca
      781 ggccgatgaa cagataggac gcgaaggcag gaatgttttg ccggataatg atctggaaaa
      841 ccacataata aatgacgatg tccatcaccg gtttcagcaa ggcccacaac aggccaagct
      901 tattttcgta gtagcggagc ttgaattcta tccgggcgag cagccaaagg cgctccagtt
      961 tatgagggtt catcatgccg cgtgcggtgg tgtatgccat atccagtcgg tttttttctt
     1021 gaggagtaaa atgaattttt taagggcagg atgccgtctg gcgatccgct tcacagcgtt
     1081 gacccagttc cagcgatccc ggctgctgtt aacatataat tgcaacgtag gattctcgaa
     1141 aaggtagccc gggccgttgc tgttcagcaa ttggatgacc tcggccatat acggctgata
     1201 aaactcgcgg tgcttttcgg tgatatacgt gtgtgcaagc aacaatttgt tccggttcat
     1261 ggcgcggatc atcgagcctt gcctgacgcg gtaacgatgc agcggctcgg gcaggaccac
     1321 cccgttgaag gaatgacggg ccatgcggac gacgctttca taatcttcca ggccgaatac
     1381 cagctggccg tcattcaggc cggcttggag gaaggcggct gttttgtaca ccagcgcact
     1441 gctgtttatg ggattatgcg cgagcaggta tggccgctcc ggcgggaatg ccggccagac
     1501 tccggcggag tccccgaaat actcgaccca ggacccgacg aaataaacat ctttgtattg
     1561 atccaggacg cgaatggcct tttcataata gtcgggccgg accgcgtcgt cggcatccag
     1621 gaagctgaga taggcgcctg ccgcctgagc cgcgcccgca ttgcgggctt gcgcaagccc
     1681 gcggttcggc gtatcgatga cggtgatggc cggatgcttc cgccagcgtt cgagggcggc
     1741 caggctgccg ggctccgagg acccgtcgtt aacgatgatg atttgcgtcc gctcccaccg
     1801 gctgttcagg atggacagga cgcactcgtc caggtaggct ccgagattgt agtaagggat
     1861 gaccacggag agcatattgc tgatgaacgg cttttccggc aggggcgccg gcgcgggccc
     1921 cggccgaagg aatggaaata atttccgccg gctatacgtc tttcgcagtt ctttcaataa
     1981 ggcgatcttc ttcggcgcga tgagatcata atcatagcgg tcgagggaag cgcgggcggt
     2041 ccgcccgatg cgcaggatct cttcatcgct aagcgccagg acctgcttca acttccggcc
     2101 aaaggactcc ggctcgcggt gatcgaataa gaagccgttc accccatccg taatgatttc
     2161 gctctgcccg ccctggactg aagccaggac cactttgccg caggccatcg tttccaggca
     2221 aacgaacggc aggttgtcga cgacgctggg tacgaggacc acgtgggcat cggcaagcgc
     2281 cgtaggaatc gcggcaggat cgatcttgcc gtggaggcac aacagctttt tccggatata
     2341 cgacccgtac cgcctatgca ccaactgacc catggtcagc agttccggct gatagacaat
     2401 gtcgtcgccg ccgatgatat gcagcggatg gggaaagcca tcgtcccata gcgccttgaa
     2461 ataggccagc agttcaaaac tgcccttctg cggggagagc ttgccgtaat acacgatttt
     2521 gttccgtcga acggcgcggt cccggtcgcc tgcggccgaa tgagggtcgc aaggccggta
     2581 agcataggga agcacgatgg gccgctggcc atagtcgaga tgccgcccga cctccgccgc
     2641 catgaagcgg gagggtgaga tgaccatgtc agccgaccgg atgctgtgct tttccatttc
     2701 acatgtccag aaatccggga accggtagac ggaagtcccg ttatatggca ggtacacgaa
     2761 cgcgggagca tggatcgtga tgagcaccgg gatatcttta aaactcggat agccgaggag
     2821 ttgaaattgt tggatataat aggcgatgcc caggtagtcc tggctttcga taatatccgg
     2881 ttcgccgagc tccctgacca ttagttgggc catccgcaga aaagcgtagc tcaggccggc
     2941 ggctccgccg agctcgcgcg gcacgccgcg acgcgccgga ttgaaacggg cgatcaccag
     3001 atgccgttga aaggatgtga gggaatagtc atccacgctg tcatcggcga cgatcaccgt
     3061 gacggccacg cctttgccgg ccagcatttc ggccgtacac cggcaatagg tgctgatgcc
     3121 gcccccgtga gccggaggaa attcagttgt caatagccaa tacttcactc tttatatttt
     3181 tttacgcttt tgtcatataa agaccggaaa ctccgcttgc cctgcagcac cttgatgata
     3241 tgccccaggc gtttgtacca gaggggcagg atctcgtatt cgttgcggta aaagcgaagg
     3301 atgtattcca cctcattttg tttgctggcc gcctcgagca gttgctgccg tatccggctt
     3361 tcttcggcca attgcagctt ttcgcggctt agccgcgtca ggcatcgcct cgcctgcctg
     3421 ctgtcgtcgg ccaggcggta caacaggggg tgttccgccc tcagctgcgc atccgcctgc
     3481 tggataaggg caaaagtgtc gggcgcatct tccagcagga aaatgtcacg cggcgaatag
     3541 gatgtcaggc aataacggta ataccggtcc gccacgccgg ccgtcgccgc ggaataggtg
     3601 tagatggccg gccgaacccc gccatcggtg ctttgctccc cgggaaaata aagtgctgct
     3661 tccagtcgct gctggttaaa gagttccgtc aggcccgccc gggcagcagc ccgctcgcgg
     3721 cccggaacat aaacgaacaa cggtttcgcc cactcggcgt attcataaaa gggatgaaag
     3781 aaaagcagga gcaggcggcg tgccgccccc acaacctcct cgccgcaggc gccctccaac
     3841 gcaagacaga acgggtagtt tccccgcgaa ctgtattgat gacgtaacag cggtagggtg
     3901 tcggtcgaac cggaatggac gacatggcca accgggattc ctgccgctga cgccatccgg
     3961 ccggctgtct ccgccgtttc cggcgcatgg ttaagatagt agaagaagtt cagcatcaga
     4021 actcttttag tttgcacccc gccgaaagcg ctatccacga aagcatcctc tcccagcaat
     4081 gcgtcatcgt gccctgctcc tggtccaata cgttccccgc ttccagggtc gcccgaaccg
     4141 ccagggcgtc ttcgcgggaa aagaaatccc gcagcaagga ggtccggatc cagaacatcg
     4201 ttccggcaac aaatctcaaa tcggcatccc cgatcgaata ttttttcatc atcccggtca
     4261 agagctccga attcttggta gcgaagttgc cggagcccag gtactcattg cgcacatggc
     4321 cggcagcaca ggcaatcccc atcccggcgt ccctgtcgaa cgctgcaccg atccgccgga
     4381 atttttccgg gctgacaatg ctgaagagcc cgtccttttc ccatcggccg gaatgcttct
     4441 gatagctacg cttgtcatgc agaaagatcg cgtatggcgt gtctgggtac agccggagaa
     4501 gcaattcaac gaggatcagc tttcccccga tatcctttcc gacattggca gccacgacga
     4561 atttctccga atccccctga ggcggccgca aaccggcgaa tttatcgggc agcgagcaga
     4621 gcaggaggat gccagggtcg tcgagcgctt cgatcttcga ccgcagctcg tcaatgccgg
     4681 cctcgtcgta gtagttgtgg tacaatatgg agtatcgata gcgttccatc aggtggcgat
     4741 tgttttgttt ttagcgccgc tccagcctct tttgcccatc aggatgcgga tcagctgccc
     4801 gaacgactta taccatcgcg gcaatacctc gtattcctta tcgtaccatc cctggatctc
     4861 gtccgccttc gtgccggccg ccgggacata attcacgtcc tccaggagga ggcggatcct
     4921 tttggttccc cggatgacct tcaataactg gccgaccgcc ttgtaccacc ccggaaggga
     4981 ttcatactgt tcccggtacc aagtgaaaat ttcgtattcc ttccgggtag cgaagccacc
     5041 aaacgccggc cggatcgccg gcgccggctt ttcgaaccag agcggggccg cgggcacata
     5101 ccgcttccag agaaaatccg cctgcgacag cgcacccgcc cccggcgggg gcgcccagta
     5161 caggatcttc tggccacaat agtcgtcgtc gagcgaatag ccggcaagca tgtcgaggtc
     5221 ggcctgcgtg ggcgcttcga gaaaggtctg aaacggcgtg agcaggaagt ccgcgtcatc
     5281 cgcggtgatg gctgcgacat ctgtgagctc catcaaatcc cggcagtaat gaaggatgcc
     5341 ttcgtggacg atcggcagct tcatcaattt gtatttctcg tcggtactaa cgggtgcgta
     5401 taccggccgg tgagcgccgt caggtttttg tgcataacaa caaaccggtc cccggacgga
     5461 agaaaggatc atttcataga acaagctgta tttgtcgatc gaaagtttct tcaatttcgg
     5521 gtcgatccgg tccccgaaca gcccggttgc ccgcacggcc gggtttctca cgagcgtatc
     5581 aaacgtatca tacagcacca tataataccc gtgcaccggc tcgacgttac ccccggccct
     5641 tcccgtcaca tcgttgagga tacgttgcag ggtacccgaa tagccgatgt cgactaccgc
     5701 ctttttaccg ggatcaaaaa ggccggcttc ccgtaaatag cccaggcagc attcctgctc
     5761 actactgaaa tgggtgcggc agttttcgag cagggca
//
LOCUS       DATGKE010000481         3909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_851121, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000481 DATGKE010000000
VERSION     DATGKE010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851121"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VKU83_08330"
     CDS             complement(<1..1041)
                     /locus_tag="VKU83_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HLI93601.1"
                     /translation="MEKIVAGIITIGDELLIGQVVDTNSAWMAQELNRIGIWVARRTA
                     VGDTADDIRQALDEESRRAQIILITGGLGPTADDITKPLLAEYFGGRMIMDAGALENV
                     RSIFSRLQRPMIDRNLRQAEVPDTCTVIPNRQGTAPGMWFERDSRIFVAMPGVPHEMK
                     GMMSAEIIPRLLARFSLPFIAHRTLLLAGVGESFLSEHLASFEAGLPATVRLAYLPNF
                     GMLRLRLTIRGGDPERSATTLDSLFAALRQEVKDWLVTDQDIPIAEAVGRLLKVQGKT
                     LATAESCTGGYIAHLITSIPGSSQYFKGSVVSYANEIKSGLLDVSPEVLASEGAVSLA
                     TVEAMVKGALGRL"
     gene            complement(1067..1495)
                     /locus_tag="VKU83_08335"
     CDS             complement(1067..1495)
                     /locus_tag="VKU83_08335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLI93602.1"
                     /translation="MFTSETKIRVRYAETDQMNVVYHGNYAQYFEVGRVESIRQLGLS
                     YRDVEASGVIMPVIEWTAKFIRPAHYDDLLTIRTTIKKWPVDYRIEFHQEVFNEGGTL
                     LTTGKVLLYFLKAATRETTGMPEEMARTLRPFFSTSRTGP"
     gene            complement(1528..1938)
                     /locus_tag="VKU83_08340"
     CDS             complement(1528..1938)
                     /locus_tag="VKU83_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLI93603.1"
                     /translation="MNRFFSTQYSEGSFNLAVLIIRLVFGLQLLFDHGLGKITHFSSL
                     EYSFFDFLNIGHRWSLVLCIFAEVFCAGMVAIGLFARFAALVLVINFCVASFMALKGQ
                     PLAGHEAAFGYLACFFALLLTGPGRFSVDGAMGK"
     gene            2112..>3909
                     /locus_tag="VKU83_08345"
     CDS             2112..>3909
                     /locus_tag="VKU83_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLI93604.1"
                     /translation="MKHTAFILFLVILRASSSAQQTHYYDDPQAYFRQGKEFFEHGYY
                     SLAYPIFRDLKYSLRETDRSNNAVEYQDVKYYYLVCALEQNDQTAVAAAEDYINLENN
                     APRVGMLSFHLAEYYFRQKDYVAAARLYENADIDNLGNDEIAAMKFHQGYCYFTQKDF
                     DKAIPLFNAIRQMPRDPNYIDANYYYGFISFYRKDYPEALAAFTVVQDNPAYERVVPY
                     YVANIYLVQGKRDQAIEYAEARLKRGNQYYESELRQLVGHGYYEKQDFAKALPFLEQY
                     VGGSRKVSREDLYELSYCYYQTRNWNKAIEGFKQLGGKEDSLAQNSMYLLGDAYLKTG
                     QKGNARNAFLFCSLNSSNSRQAEISRFNYAKLSYELGYQDVALSELQNFLRLYPNSEY
                     AQEGRELLISVLAGTNNYKDALALLDSLNHPSENARRLIPRVLYGRATELINDGMLPE
                     ADALLDQALKDPDNGSVLPYIEFWKAEIAYRLNRIDDAIRYYLAYLKSGAVNGEVNPA
                     DARYNLGYCFLKKENYRQAQGFFEQVARTPRLTSSPLEQDAYVRDADCYYMERDYRTA
                     LNMYNKVLDLSWPAGDYATFQKAMIAGVSNDRQ"
BASE COUNT          916 a         1000 c         1133 g          860 t
ORIGIN      
        1 cagccggccg agggcgcctt tgaccatggc ttcgacggtc gcaaggctga cggcgccttc
       61 tgaggcaagg acttccgggc tgacatcgag gagacccgac ttgatctcgt tagcgtagct
      121 gacgacactc cccttgaagt attggctgga acccgggatg gaggtgatga ggtgggcgat
      181 atagccgccg gtgcagcttt cagccgtagc cagcgtttta ccctgcactt taaggagccg
      241 gccgacggct tccgcaatgg ggatatcctg atcggttacg agccagtctt tgacctcctg
      301 cctgagggcg gcaaacaggg aatcaagggt cgtcgccgat cgttccgggt cgccgccccg
      361 gatggtgagc cggagccgga gcatgccgaa gttaggcagg tatgccaaac ggaccgtggc
      421 cggaaggccg gcctcaaagg aagcaaggtg ttccgaaaga aacgactccc cgaccccggc
      481 gagcagtagg gtgcggtggg cgatgaatgg caggctgaaa cgggccagta gccgggggat
      541 gatctcagcc gacatcattc ctttcatttc gtgcggcacg ccaggcatcg cgacgaagat
      601 gcggctgtct ctttcaaacc acattcccgg tgcggttccc tgccggtttg gaatgacggt
      661 acacgtgtcg ggcacttcgg cctgccggag gttacggtcg atcatcggtc gctgcaaccg
      721 ggagaaaatg gacctcacat tttccagggc gcccgcgtcc atgatcatcc ggccgccgaa
      781 atattctgcg agcagcggtt tcgttatgtc gtccgctgtc ggccccagac cgccggtaat
      841 gaggatgatc tgtgcccgcc gggattcttc gtcgagagcc tgccggatat catccgcggt
      901 atcgccgacc gccgttcgcc ttgctaccca gatgccaatc ctgttcagtt cctgcgccat
      961 ccaggcgctg ttcgtatcga ccacctgacc aatcaataac tcatctccaa tcgtgatgat
     1021 gccggcaacg atcttttcca tgcaataaag gtagggaatg aaatgatcag ggaccggtcc
     1081 gggaggtcga aaagaatggg cggagcgtcc gggccatttc ttcgggcatt ccggtggttt
     1141 ccctggtcgc cgccttcagg aaatagagca ggactttgcc cgtagtgagc agcgtgccgc
     1201 cctcgttaaa gacttcctgg tgaaattcga tccgataatc caccggccat tttttgatgg
     1261 tcgtccggat ggtcaggaga tcgtcgtaat gggccggacg gataaatttg gcggtccatt
     1321 caatgacggg catgatgacg ccggaggctt cgacatccct gtaactcagg cccagttgac
     1381 ggatcgattc aacgcggccg acctcgaaat actgggcgta gttgccgtgg tagactacat
     1441 tcatctggtc ggtctcggcg tagcggacgc ggatcttcgt ttcgctcgtg aacatgcatt
     1501 atttgggttt ggatgggggc gttgcgatca cttacccatg gcgccatcga cgctgaaccg
     1561 gccggggccg gtgagtaaaa gggcaaagaa gcaggcgaga tagccgaacg ctgcttcatg
     1621 gcctgccagg ggctgaccct tgagggccat aaaggaggcc acgcagaaat tgatcaccag
     1681 gaccagggcg gcgaatcgcg cgaagagacc tatagcgacc attccggcgc agaacacctc
     1741 ggcgaaaatg cagagtacca gcgaccagcg atgaccgata ttcaggaaat caaagaagga
     1801 atattccagg ctgctgaaat gggttatttt tccgaggccg tggtcgaaca ggagttgcag
     1861 tccaaagacc agccggatga tcagaacagc aaggttaaaa ctgccttccg aatactgcgt
     1921 ggagaagaat ctattcatgg gcgatggggt tttatgctaa gttactcgaa agaaactatt
     1981 tttaaagaaa tgttaattga tgcacttatc cacacgtgtt tggaacgatc tttggctaaa
     2041 ggcaaaataa ttagaatctt tagcgcgttg gaaattgcag ggggtggaca ataaatcaaa
     2101 ctatttttcc tgtgaagcat actgccttca ttcttttcct ggtcattctc cgtgcttcgt
     2161 cgtcagccca gcagacccat tattatgacg atccgcaggc gtatttccgg cagggcaagg
     2221 aattttttga acacggatac tatagcctgg cctatcctat cttcagggac ctgaaatatt
     2281 cgctgcgcga gaccgatcgc agcaataatg ccgttgaata ccaggatgta aaatactatt
     2341 acctggtatg tgcgctggag cagaacgatc agaccgcggt ggctgcggca gaagattata
     2401 tcaatctcga aaacaacgcg ccccgggtgg gaatgctcag tttccatctg gccgaatatt
     2461 atttccggca gaaggactat gtcgccgccg ccaggcttta tgaaaacgcg gatattgaca
     2521 acctcggcaa tgacgagatc gctgccatga agttccatca agggtactgc tattttacgc
     2581 aaaaggattt tgataaggcc atcccgcttt ttaatgccat ccgccaaatg ccacgggacc
     2641 ccaattatat cgacgccaat tattactatg gttttatctc cttctaccgg aaggattacc
     2701 cggaggcgct ggccgccttt acggtcgtgc aggacaaccc ggcctatgaa agggtggtgc
     2761 cgtattacgt ggcgaatatc tacctggtcc agggcaagcg ggaccaggcc attgaatatg
     2821 ccgaggccag gttgaaacgc gggaatcagt attacgagtc ggaactgagg cagctggtag
     2881 ggcatggata ttacgaaaag caggatttcg ctaaggccct gcctttcctg gaacaatatg
     2941 tcggcggatc cagaaaggtt tcccgggagg acctgtatga gctgtcgtat tgctattacc
     3001 agaccaggaa ctggaacaaa gcgatcgagg ggttcaagca gcttggcggc aaagaagatt
     3061 ccctggccca gaattcgatg taccttttag gggatgctta cctgaagacc gggcagaaag
     3121 gtaacgcgcg aaatgccttt cttttctgtt ccctgaacag cagtaattcc cggcaggccg
     3181 agatttcacg gttcaattac gcaaaattgt cgtatgaact gggttaccag gatgtggcgc
     3241 tgtcggagct gcagaatttc cttcgcctgt accccaattc cgagtacgcg caggagggca
     3301 gggaactttt gatcagcgtg ctggccggta ccaacaatta caaggatgca cttgccctgc
     3361 tggacagcct gaaccaccct tcggaaaacg cgcggcggct gattccgcgg gtcctgtatg
     3421 ggagggctac agagctgatc aatgacggga tgttgccgga ggcggatgcg ctgctggacc
     3481 aggcattgaa ggacccggac aatggcagcg tactgcctta tatcgaattc tggaaggccg
     3541 aaatcgctta ccggctcaat aggatcgatg acgcgatccg gtattacctg gcatacctga
     3601 agagcggcgc cgtgaacggt gaggtaaatc ctgctgacgc ccgctacaat ttggggtatt
     3661 gtttcctcaa aaaagaaaat taccgccagg cgcagggatt ctttgaacag gttgcgagga
     3721 cacccaggtt gacgtcgtca ccgctcgagc aggatgccta tgtgcgggac gctgattgtt
     3781 attatatgga gcgggactac aggacggcgt tgaacatgta caataaggtc ctggaccttt
     3841 cctggcctgc cggcgattac gcgaccttcc aaaaggcgat gatagcggga gtcagcaatg
     3901 atcgccaga
//
LOCUS       DATGKE010000482         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1117806, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000482 DATGKE010000000
VERSION     DATGKE010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117806"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VKU83_08350"
     CDS             <1..1270
                     /locus_tag="VKU83_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93605.1"
                     /translation="ETGQPQLRAYKEQSAAASDNIALARNTLVPGLTAGYQAEYATDN
                     NITGMSYPGLLMPISGPVALHSNGNFLPGTALAAYLQWTPITFGQRAAAVERAAAQFK
                     LSGSAYDNALFQQRYSGILTYISIVYLQKLLSTEQANIHRTQVGLSQSLELAKQGLRP
                     GLDTVQFQSLLAQAVMERLNTQRLYTSQVLELCRLTGMPDRPADIIFSDTALALRLPA
                     LPDTSGGYTQNPAYRYYQAQIGLSAANLKEIERGWVPRLDVWANAYSRGSGIDPNGAV
                     NKPDGWALSRTNYGAGLQLSFPILQFSRVNIQKRQYRSRLAADQDQLAQVDLNLRRQV
                     ETAESNYEQSLLLAREAPVQTRAARLSFEGLALSYRNGLVDFTGLTQGQYQLLHAEMT
                     EADANLQVWRALLDISAAKGNLQLFTDQLK"
     gene            1277..>2721
                     /locus_tag="VKU83_08355"
     CDS             1277..>2721
                     /locus_tag="VKU83_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI93606.1"
                     /translation="MKLIISALRHPITILVAVIAILFFSWLAVRNSKVDIFPKLGLPT
                     VYVAQPYGGLSPDQMEGFITSYYEYHFLYITGVKYVDSKSIQGAALIKIEFNEGTDMS
                     QAMAEVVGYVNRSRAFMPPGTVPPFIMRFDAGSVPVGQLVFSSDSRSLSEISDLALFR
                     VRPMFSTLPGVSAPPPFGGNQKTVVVTVDPQKLQGYHLSPDEVVQSLIRFNSISPAGN
                     VSIGDTTYFTPQNSVIEDVQDLQKVPLRTGSGPGVYLGDVASVALGADITSSYALVNG
                     KRSVYIPVTKRADASTWDVVQRVKAALPDMQAAIPADIKVSYEFDQSGYVLHSLNSLL
                     FEGGLGALLTGLVVLLFLKDPRSGLIVIMTIPLALLSAATALYLCGQSINIMTLGGLA
                     LSVGILVDEATVTIENIHRHQESGKDKARAIADACLEIAAPKLLILLSILAVFVPALF
                     MNGIPKAMFLPLSLAVGFAMIASFLLSQTFVP"
BASE COUNT          534 a          891 c          730 g          566 t
ORIGIN      
        1 agagaccggc cagccgcagc tccgcgccta taaggaacag tccgcggccg cctcggataa
       61 tatcgccctt gcccgcaaca ccctggtgcc cggccttacg gccggttacc aggcggagta
      121 cgccaccgac aacaacatca ccggcatgag ctatcctggc cttctcatgc cgatctcagg
      181 gcccgtggcc ttacacagca acgggaactt cctccccggc acagccctgg cggcctatct
      241 tcaatggacg cccatcactt tcggccagcg ggccgccgcg gtggaaaggg ccgccgctca
      301 gttcaaatta tccggcagcg cctacgacaa cgcccttttt cagcaacgct attccggaat
      361 tctcacgtat atcagcatcg tgtacctgca aaagctgctc agcaccgagc aggccaacat
      421 ccaccgtacc caggtcggcc tcagccaatc gctcgaactc gccaaacaag ggctccgtcc
      481 cggtctcgat acggtccagt ttcaatcgct cctcgcccag gccgtcatgg agcgcctgaa
      541 cacccaacgt ctctatacgt cccaggtgct ggagctctgc cgcctcacgg ggatgccaga
      601 ccgcccggct gacatcatct tttcggatac tgccctggcg ctccggctgc cggcattgcc
      661 ggatacgagc ggcggctata cgcagaaccc tgcctaccgg tactaccagg cgcagatcgg
      721 gctcagcgcc gcgaacctga aggaaatcga acgcggctgg gtgccgaggc tggacgtctg
      781 ggccaatgcg tattcccggg gatccggcat cgatccgaac ggtgcggtca acaaaccgga
      841 cggctgggcg ctgtcccgca ccaactatgg ggcgggcctc caactcagtt ttccgatcct
      901 ccaattttcc cgagttaaca tccagaaaag gcagtaccgt tccaggctcg cagccgatca
      961 ggaccagctt gcccaggtgg atcttaatct ccgccgccag gttgaaacgg ctgaatccaa
     1021 ctatgagcag tccctgcttc tggcgcggga agctcctgtc cagacgcggg cggcccggct
     1081 ctctttcgag ggcctcgccc tcagttaccg gaacggattg gtcgatttta ccgggctgac
     1141 acaaggtcaa tatcagctcc ttcatgcaga aatgacggaa gcggacgcca acctccaggt
     1201 atggcgcgcc ttattagaca taagtgcagc aaaaggcaat cttcaactct ttaccgacca
     1261 attgaaatag cctgccatga agctcattat ctccgcactg cgtcatccca taacgatcct
     1321 ggttgccgtt atcgccattc tctttttctc ctggctggcg gtccgcaaca gcaaggtcga
     1381 cattttcccc aagctgggtt tgccgacggt ctatgtcgcg cagccttacg gcgggctttc
     1441 gcccgaccag atggagggct tcatcacttc ctactacgaa taccatttcc tgtacattac
     1501 cggcgtcaag tacgtggatt cgaagagcat ccagggcgcc gcgctgatca agatcgaatt
     1561 caatgaaggt acggacatga gccaggcgat ggcggaagtc gtcgggtatg taaaccgctc
     1621 gagggctttt atgccgcctg gcacggtgcc gcccttcatc atgcggttcg acgccggcag
     1681 tgtgccggta ggccagctcg tcttcagttc ggacagccga tcgctaagcg agatcagcga
     1741 cctggcgctg ttccgggttc gcccgatgtt ctcgaccctg ccgggagtaa gcgccccgcc
     1801 gcctttcggc ggcaaccaaa agacggtagt cgttaccgtg gaccctcaga agctgcaggg
     1861 gtaccacctt tcgcccgacg aggtggtcca atccctgatc agattcaaca gcatttctcc
     1921 ggcgggcaat gtcagcatcg gcgacactac ctacttcacg ccgcagaaca gcgttatcga
     1981 agacgttcag gacctgcaaa aggtcccgct gcggacagga agcggccccg gtgtttacct
     2041 tggagacgta gcctcggtcg cccttggcgc cgatattacg agcagttatg ccctggtcaa
     2101 cgggaagcgt tctgtttata ttccggttac gaaacgcgcc gacgcttcta cctgggatgt
     2161 cgttcagcgg gtgaaggcgg ccttgccgga tatgcaggct gccatcccgg ccgacatcaa
     2221 agtctcttac gagttcgatc aatccgggta tgtcctccat tccctgaaca gccttctttt
     2281 cgaaggcggg ctgggcgccc tcctcaccgg cctggtggtg ctgctcttcc tgaaagaccc
     2341 gcgcagcggc ctgatcgtta tcatgaccat cccgttggcg ctgctgtcgg cagccaccgc
     2401 gctttacctc tgcgggcagt ccatcaacat catgaccctc ggtggcctgg ccctttccgt
     2461 cgggatcctt gtcgacgaag ccacggtaac catcgaaaat atccatcgcc atcaggaatc
     2521 cgggaaagac aaggcgcgcg ccatcgccga cgcctgcctg gagatcgccg cacccaaact
     2581 gctcattctg ctgagcatcc tggccgtttt tgttccggcc ctgttcatga atggcattcc
     2641 gaaagccatg ttcctgccgc tttcccttgc ggtcggattc gcgatgatcg cctcattcct
     2701 cctgtcgcag acttttgttc c
//
LOCUS       DATGKE010000483         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1472388, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000483 DATGKE010000000
VERSION     DATGKE010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472388"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VKU83_08360"
     CDS             <1..367
                     /locus_tag="VKU83_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HLI93607.1"
                     /translation="DTPYWKYLLLSISGYELYLKTYRTGFDARNVVEQIVLNEDFPRS
                     VIYSVNRLYQYFERLKSDGNKEAWERLEFMIGRIRSKVKYSTVDSILREGLHVYLSGV
                     KEALYEVGNALNQHYFAYS"
     gene            381..1343
                     /locus_tag="VKU83_08365"
     CDS             381..1343
                     /locus_tag="VKU83_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLI93608.1"
                     /translation="MPRFSIHHITKYTYEGPVRDSANQIVLFPVKDEYQDVLRQDLSI
                     TGDPPVEIYKDYYGNEVGSFTHAEPHTSLTIDSSVEVVTRSRPALQDTADKEMQWALL
                     EQIRWQVPYIDFLKQEQFSSLAEVLESVCPDKARTQTPLAAALQLRTYVYETFKYIKG
                     LTSVETTLDEVWKLKAGVCQDFAHMLLVFLKQIQIPARYVSGYICTNKNGMRGEGATH
                     AWIEAYIPFNGWVGLDPTNNCIADDLHVRLAMGRSFSDCSPVKGTYKGSAGQQLEVGV
                     SVSYENGRISEDAVTVLSPQPSANGKTDNSYRRYMEMMQQQQQQ"
     gene            complement(1368..2483)
                     /gene="dnaN"
                     /locus_tag="VKU83_08370"
     CDS             complement(1368..2483)
                     /gene="dnaN"
                     /locus_tag="VKU83_08370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLI93609.1"
                     /translation="MKFIVSSSALLKQLQQISGVINANTVLPILEDFLFEVEKNKLTV
                     VATDLETVMKVHLDVEAKDSGKVCIPAKILIDSLKNIPDQPLTFNIDKHFAVELTSDN
                     GKYKVMGENPDNFPKEPASDETTSFTATSSALVTAINKTLFAVSNDDLRPAMTGVFFE
                     MDKKGLQFVATDAHRLVRYKRKDIACPKNDSLIVPKKPLNLLKSALPLNEDEITISYN
                     GNHLFVSHGTTQMSCRLIDARFPDYKVVIPTDNPYKLTVGRNDFQGALRRVSIFSNKS
                     TNQVVLNIHGSELQLMAQDVDFSFEGNERMKCQYDGEDLAIAFNARFLIEMLASADSD
                     EVRMELSTPTKAGIIKPVEEDENEDVLMLVMPLMLNS"
BASE COUNT          664 a          727 c          761 g          671 t
ORIGIN      
        1 ggatacccct tattggaaat atctgctgct ttccatctcg gggtacgagc tttatctgaa
       61 gacctaccgg accggtttcg acgcgcgaaa tgtcgtagag cagatcgttc tcaacgaaga
      121 tttcccccgg tcggtgatct attcggtaaa ccggctatac caatatttcg aacggctgaa
      181 gagcgacggc aacaaagaag cctgggaaag actcgagttc atgatcgggc gcatccgcag
      241 caaagtcaag tactctaccg ttgattctat cctccgggaa ggattgcatg tttacctgag
      301 cggagtaaag gaagcgcttt atgaggtagg gaatgccttg aatcagcatt attttgcata
      361 ttcctagtta cttttaccgt atgccccgtt tcagcatcca ccatatcacg aaatatacct
      421 acgaaggtcc cgtacgggac agcgccaacc agatcgttct gtttccggta aaagacgagt
      481 accaggacgt tctccggcag gacctctcca tcaccggcga tccgccggta gagatctata
      541 aagactatta cggcaacgag gtcggttcct ttacgcatgc cgaacctcat accagtctga
      601 ccatcgattc gagtgtcgag gtcgtcacca gatcgaggcc ggcgttgcag gacacggccg
      661 acaaagagat gcaatgggcg ctgctcgaac agatccgctg gcaggtaccc tatatcgatt
      721 tcctcaaaca ggagcagttc tcttcgcttg ccgaggtgct ggaaagtgtt tgtcccgaca
      781 aggcgcgcac tcagacgcca ctagcggcgg cactgcaact gcgaacctac gtctacgaga
      841 cgttcaagta cataaaaggc ctgacgagcg tggaaacgac cctcgatgaa gtatggaagc
      901 tcaaggccgg ggtctgccag gatttcgctc atatgctcct ggtctttctt aaacagatcc
      961 agatcccggc ccgctatgta agcggctata tttgtaccaa taagaacggg atgcggggag
     1021 agggtgctac acatgcctgg atcgaagcct atattccttt caacggctgg gtgggtctgg
     1081 atcccaccaa caactgcatt gccgacgatc tgcacgtgcg gttggccatg ggccgcagct
     1141 tttccgattg ctcgcccgtc aaaggaacgt ataaaggaag cgctggtcaa cagctggagg
     1201 taggcgtttc cgtatcctat gaaaatgggc ggatatcgga agacgccgtt acggtgctct
     1261 caccccaacc ttcggcgaat gggaaaacgg acaactctta tcgccgatac atggagatga
     1321 tgcaacagca acagcagcag tagacaacgg aaaacggcag aagccgatca gctgttcagc
     1381 atcaggggca tcaccagcat cagcacatct tcgttctcgt cttcttctac cggcttgatg
     1441 atacctgcct tagtaggtgt cgagagctcc atccgcactt catcgctatc ggcggaggcg
     1501 agcatttcga tgaggaagcg ggcattgaag gcaatggcca ggtcttcgcc atcgtattgg
     1561 cacttcatgc gttcgttacc ttcgaaggaa aagtcgacat cctgcgccat cagctgcagc
     1621 tcgctgccgt ggatgttcaa aacgacctgg ttggtgctct tgttgctgaa gatgctcacg
     1681 cggcgaaggg ctccctggaa gtcattccgg cctaccgtca atttgtaggg gttgtcggtg
     1741 gggatgacga ccttgtaatc ggggaagcgg gcatcgatga gacggcagct catctgtgta
     1801 gtgccgtggc ttacgaaaag atggttgccg ttatagctga tcgtgatctc gtcctcgttg
     1861 agcgggaggg cggatttgag aagattcagc ggttttttgg gaacgataag agaatcgttt
     1921 ttcggacagg cgatatcctt gcgtttgtac cgcaccagcc ggtgagcatc ggtggctacg
     1981 aactgcaatc ctttcttatc catttcgaag aatacgccgg tcatcgcagg gcgcaagtcg
     2041 tcgttgctca cggcaaacag cgtcttgttg atggccgtca ccaatgcgga agaggtagcc
     2101 gtaaacgagg tggtctcatc gcttgccggt tccttgggaa aattatccgg gttctcgccc
     2161 atcaccttat acttgccgtt gtcgctggtc agctccacgg caaaatgttt gtcgatatta
     2221 aacgtaagcg gctgatcggg aatgttcttg agcgagtcga tgaggatctt ggcggggata
     2281 catactttgc ccgaatcctt tgcctcgaca tcgaggtgca ccttcatcac cgtttccaga
     2341 tcggtggcga caacggtgag cttattcttt tccacttcga acaggaaatc ttccaggatg
     2401 ggcaatacgg tatttgcatt gatcaccccg ctaatctgtt gcagctgttt caataatgcc
     2461 gaggaggaaa cgataaactt catggaattt gcgtctttgt ggcgaaacta cgttttttta
     2521 cgtaatagcc aatagattag aggcggcgcg ccgccattct ccaccaattg ttaatttcct
     2581 gtgactaacc cgtgccccgg ccgcaaggcc gcggacgagc ggcaagagcg aagttcgtag
     2641 ttttgccgga tgtctccgac gcgcaaagct ctcaaccccg aacaggccct tcagaaagcc
     2701 cgtcattatt gcggctacca ggatcgttgt cattacgagg taagagaaaa gctttattcg
     2761 ttcggtctcc ggaagccgca ggtagacgca gcgctggcaa cgctcattga agaaaattac
     2821 ctc
//
LOCUS       DATGKE010000484         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1453483, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000484 DATGKE010000000
VERSION     DATGKE010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453483"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VKU83_08375"
     CDS             complement(<1..369)
                     /locus_tag="VKU83_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93610.1"
                     /translation="MLKFNSCLAILATGFVLFTGCVPTSKFNAAQQALASARYDSAQL
                     ANKVKDLQGSLAQANQLATDRQHQIDSLSQQLADARAQIDSLGKVIATLKDKVNDLNA
                     ESSNRLALLSKSKQELINQQA"
     gene            complement(497..1744)
                     /locus_tag="VKU83_08380"
     CDS             complement(497..1744)
                     /locus_tag="VKU83_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HLI93611.1"
                     /translation="MQTSRKAAIGFIFVTLLIDVMGWGLIIPVMAELVSKLKGIPINQ
                     ASPYGGYLLAVFACMQFVFAPVVGNLSDRYGRRPVLLLALLGFGIDYIILALAPAYGW
                     LFIGRVIAGMTGASFTTATAYIADVSTDPTTRAKNFGLIGAAFGLGFVLGPALGALLA
                     TWGIRAPFYAAAVLCLLNCLYGYFVLPESLPKEKRRPFEWKRANPLGSLRFLFKHPEI
                     GGLAFSFFLIYLAAQSVQGNWNYYTQYRFGWSDKMVGISLAVVGVLVGGVQAGLTRVI
                     VPKIGNEKSIYLGLSFYTLGLILFAFATQGWMMFAFLVPYCLGGICGPSLQAVISGHV
                     DPSHQGELQGGLTSLMSLTTILGPLIMTQIFAYFTSSKAPFYFPGIHFLLGALCMLMA
                     VIIAGAVLSREKREKRTEEVYQS"
     gene            1911..2675
                     /locus_tag="VKU83_08385"
     CDS             1911..2675
                     /locus_tag="VKU83_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93612.1"
                     /translation="MANKTKIALVTGGSRGLGKDMALRLAEKGIDVIVTYHSREEEAK
                     AVVDRVKAGGRKAAALRLNTGDIKRFDPFFEQLKTTLKAVFDTDRFDFLINNAGIGGY
                     APFDQVSEEMFDELLNIHFKGVYFLTQKALPLLNDRGGIINISSGLARFSHPGSSAYA
                     SMKGAIEVLTRYLATELGPRGIRANTVAPGAIATDFGGGRVRDNADINKAVASVTALG
                     RAGVAEDIGGVVAFLCTEDARWINAQRLEVSGGMHI"
     gene            2771..>3796
                     /locus_tag="VKU83_08390"
     CDS             2771..>3796
                     /locus_tag="VKU83_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI93613.1"
                     /translation="MHPNISRWRYYGWFIAAFGLLWFLFKLGGITQFRAAFLSTLIDL
                     GVGGGALVLIAEGLLPRYYLRGRRGIFFWGAGLTIAATGSAIVLLQLDRMGYSLSGYP
                     EYLRRYKEHYFYWFWSDLVVGSYFLTGFIALGGTAIRLNMERGLAAAKLANLERDKLQ
                     SELDALRSPMSAHFIFNALNTLYYTIDKSNDAARELTERFSGLLRYQLYECNAPLVPI
                     GKELQLVEHFIALQKERLDPEVEVNCRGFGEVKQVYIPPYLLLPLVENCFKHLAGRDG
                     RRIDLECSFRNGCFIWRAGNTCDHRGEEDKTGGLGLAVLRKRLAILYPDRHRLTSALK
                     DGYYLATL"
BASE COUNT          854 a          936 c         1177 g          829 t
ORIGIN      
        1 ggcctgctgg ttgatcagtt cctgtttgct tttgctgagc agggcaagtc tgttcgagga
       61 ttcggcgttc aggtcattta ccttgtcttt cagcgtggcg atgaccttgc caagcgagtc
      121 gatctgcgca cgggcgtcgg ccagctgttg ggaaagggaa tcgatctgat gctggcggtc
      181 ggttgcgagc tggttggcct gggccagtga gccttgcagg tcctttactt tgttcgccag
      241 ctgtgcgctg tcatatctgg ccgatgccag ggcttgttga gcagcattga atttactggt
      301 agggacacag ccggtaaaga gtacgaagcc tgttgcgagg atcgccaggc aggaattgaa
      361 ttttagcata gtctttagtg taagctttta gcgctaatat aagtcatttg aggagctgac
      421 gcgttaaaag taatccccag ggcttagagc ggggttagaa cgaaattaga aaatcgttag
      481 aaccgacggc cgctgttcat gactgatata cttcttcggt tcgcttttcg cgtttttcgc
      541 ggctcagcac ggcgccggcg atgatcacgg ccatcagcat gcacagggcg ccgagcagaa
      601 aatggatccc ggggaaatag aacggcgctt tgctggatgt aaaataggcg aagatctgcg
      661 tcatgatcag cgggccgagg atagtcgtaa ggctcatgag actggtcagg ccaccttgca
      721 gctcgccctg gtggctggga tctacgtggc ccgagataac ggcttgcagc gaaggaccgc
      781 aaatcccacc caaacaataa ggaaccaaaa aggcgaacat catccagccc tgggtggcga
      841 aggcgaacag gatcagtccg agcgtataga acgacagacc aagatagatg cttttttcgt
      901 tgccaatttt aggaacgatc acccgggtca agcctgcctg gaccccgccg acgaggacgc
      961 cgacgacggc cagggagatc ccgaccattt tatcgctcca tccaaagcga tattgcgtat
     1021 agtagttcca gttaccttgt accgattggg ctgccaggta gatgagaaag aaactgaacg
     1081 ccaacccgcc tatttcgggg tgcttgaaca agaaccggag ggagccgagc gggttggctc
     1141 gtttccactc gaaaggcctg cgtttctcct tgggcagcga ttcaggcagc acgaagtagc
     1201 catacaggca attgagcaga cagaggaccg cggctgcgta gaagggagct ctgatccccc
     1261 aggtcgcgag gagggcgccg agggcgggtc cgaggacgaa gccgagtccg aaagcggccc
     1321 cgatgaggcc gaaattcttg gccctggtcg tggggtccgt gctgacatcg gcgatatagg
     1381 cggtggcggt ggtaaagctg gcgccggtca tccctgcgat cacccggccg atgaacagcc
     1441 agccgtaagc cggtgccagg gccaggataa tgtaatctat gccgaagccc agaagggcga
     1501 ggagcaggac gggtcggcga ccataccggt cactgagatt gcctacgaca ggggcgaaca
     1561 cgaattgcat gcatgcaaaa acggccagga gatagccgcc ataggggctt gcctgattga
     1621 tggggatgcc cttcagcttg gagaccagct cagccatcac aggaatgatc aatccccagc
     1681 ccatgacgtc gatcagcagg gtaacaaaga tgaatccgat agcggctttc ctggatgttt
     1741 gcatgagtta gctgttttgc gttgggaaag caaacatact aaaattcggg tcgaaattaa
     1801 agtagatgtt acgcgtcgta tttgaaattc gcaaatatcg gattgatggg tacaaaccgg
     1861 gtgccgactt tgcttacgat ctttgcgtta tcaataaaat aatatacgag atggcaaaca
     1921 aaacaaagat cgccctggta acgggtggaa gccgtggttt agggaaggat atggccttgc
     1981 ggctggcgga gaaagggatc gatgtgatcg ttacctatca cagcagggag gaagaagcca
     2041 aggctgtggt ggaccgcgta aaggcgggcg gccggaaggc tgcggccctg cggctcaata
     2101 ccggcgatat caagcgtttc gatccgttct tcgagcagct gaagacgacg ctgaaggctg
     2161 ttttcgatac ggaccggttc gattttttga tcaacaacgc ggggatcggc ggttatgcac
     2221 cattcgacca ggtttcggag gaaatgttcg acgagctcct gaacattcat ttcaaaggcg
     2281 tttatttcct cacgcaaaaa gcactgcctc tgctgaatga cagagggggc atcatcaata
     2341 tctcttccgg gctggcgcgg ttcagtcatc cggggtcttc ggcctacgcc tccatgaaag
     2401 gcgccatcga ggtcttgacg cgctatctgg ctaccgagct tggtccgagg gggatcagag
     2461 caaacacggt tgctccggga gcgatagcta ccgattttgg cggcggacgc gtgcgggata
     2521 atgcggacat caacaaagcg gtggccagcg taaccgcact gggccgtgca ggcgttgcgg
     2581 aagacatcgg cggggtcgta gcttttcttt gtaccgagga tgcccggtgg atcaacgctc
     2641 agcgccttga ggtatcgggc gggatgcaca tctgatcgag ggcccggatg cccttcaccc
     2701 agaaaatccg cccacctacc ataaaaccgg gtatccggtc gccgattttg gctatcttga
     2761 ctgtggacgt atgcatccaa acataagccg ctggcgatat tacggttggt tcatcgcggc
     2821 cttcgggttg ttatggttcc tgttcaagct ggggggtatc acgcaattcc gggcggcgtt
     2881 cctgtccacc ctgatcgacc tgggggtggg cggaggagca ttggtgctca tagcggaagg
     2941 tttgttaccc cggtattatt tgcgcgggcg gcgtgggatc ttcttttggg gcgcggggct
     3001 aacgatcgcg gcaacggggt cggccatcgt gttgcttcag ctggaccgga tggggtacag
     3061 tctgtcgggt tatccggaat atttgcgccg ttacaaggaa cattattttt actggttctg
     3121 gtcagatctc gtggtggggt cctattttct gaccggattc atcgcgttag gcggaacggc
     3181 gatccggctg aatatggaac gcgggctggc agcggcgaag ctggcaaacc tcgaacggga
     3241 caagctgcaa tcggagctcg acgccttgcg cagccccatg agcgcccatt tcatattcaa
     3301 tgcgctgaat acgctctact ataccatcga taaatccaac gacgcggcaa gggaactgac
     3361 agaacggttc tccgggctgc tccgctatca gctctatgaa tgcaacgccc cactggtacc
     3421 gatcggaaaa gagctgcagc tggtggaaca ttttatcgcg cttcaaaaag agcggctgga
     3481 tccggaagtg gaggtgaatt gccggggttt tggggaggta aagcaggtgt atatacctcc
     3541 ttatctgctc ctgccgctgg tagagaactg cttcaagcat ctggcaggcc gggacggccg
     3601 gcggatcgat ctcgagtgca gtttccgcaa tggctgtttt atctggaggg caggtaacac
     3661 ctgtgaccat cgcggtgaag aagacaagac cggcgggctg gggctggctg tattgcggaa
     3721 aaggctggcc attctgtatc cggaccgaca ccggctgacc agcgcgttaa aggatgggta
     3781 ttatttagcc acactt
//
LOCUS       DATGKE010000485         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1311629, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000485 DATGKE010000000
VERSION     DATGKE010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311629"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VKU83_08395"
     CDS             complement(<1..573)
                     /locus_tag="VKU83_08395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLI93614.1"
                     /translation="MQYTRFGNTGMTVSRLCLGCMTYGRPTERWPWALDEAKSRPFFE
                     KAIEIGINFFDTADVYSLGESEKVTGKALWKLASRDAIVLATKVFNPMGPGPNDRGLS
                     RKHILSAIDASLQRLATDYVDLYQIHRWDYNTPIEETMEALHDVVRSGKARYIGASSM
                     YAWQLVKAQEVAERHGWTRFVSMQPHYNLLY"
     gene            662..>2045
                     /locus_tag="VKU83_08400"
     CDS             662..>2045
                     /locus_tag="VKU83_08400"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI93615.1"
                     /translation="MRKTFGLAVAALLLLQQGTRAQSPASGSVFDQHEAFAPLFYPAY
                     GDEYRSAAGAPGPRYWQNRADYKIDARLDEGDQSISGTVLITYKNNSPQNLPFVWLQL
                     DQNIYAQGSRGEEVTEISGGRWANRNSFVGGDELKSVELINPATGKAMPADYLVSDTR
                     MQIKLPAELKAGGGILKIRIQYSFKIPEYGTDRMGRMQAKNGAWMYDIAQWYPRMCVY
                     DNVLGWNTQPYLGQGEFYLEYGDIDYSVNVPGNMIVGGSGELLNPAEVLTAKEQRRLA
                     EAKNSDKTVAIRGKDEVDDPASRPAQNRLTWKFRCTNTRDVAWGASSAYVWDAARINL
                     PSGKKALAESVYPVEVSSDSAWGRSTEYVKGCIEFYSKYLYEFSYPSATNVAGIVGGM
                     EYPGIVFCSSRARGRGLWGVTRHEFGHNWFPMIVGSNERKYPWMDEGFNTFINTIADS
                     AFNNGEYWSKD"
BASE COUNT          458 a          518 c          617 g          452 t
ORIGIN      
        1 atagagcagg ttgtagtgcg gctgcatgga tacgaaccgg gtccagccat gccgctccgc
       61 gacttcctgc gcttttacga gttgccacgc atacatggag ctggcgccga tatagcgggc
      121 tttcccggac ctcaccacat cgtgcagggc ctccattgtt tcttcgatcg gtgtgttata
      181 atcccagcgg tggatctgat agaggtcgac atagtccgtg gccagtcgct gaagactcgc
      241 gtcgatcgcg ctgaggatat gtttccggga cagtccccgg tcattcggac cggggcccat
      301 tggattgaaa accttagtgg caaggacgat ggcgtcgcgg gatgcaagct tccaaagcgc
      361 cttgccggtg accttctcac tttccccaag ggaatagacg tcggccgtgt cgaagaagtt
      421 tatcccgatc tcgattgcct tctcgaaaaa agggcggctt ttcgcctcat cgagcgccca
      481 tggccagcgt tcggtgggcc tgccataggt catgcaaccc aggcagagcc tcgaaacggt
      541 catgccggtg ttgccgaatc tggtgtattg catgtcattg tcaaacaaaa tatatgatgt
      601 atggtttagg ccttgggccg cctttaccta catttgctca cataataaac attgtttaca
      661 tatgagaaaa acttttggtt tggctgtggc ggcactgctg ctgctccagc agggtaccag
      721 ggctcagtcc cccgcatcgg ggtccgtatt tgatcagcat gaagcttttg ctcccttgtt
      781 ttatccggca tatggcgatg aataccggtc tgcagcgggt gcaccgggcc cacggtattg
      841 gcagaaccgg gccgattaca aaatcgatgc ccggctggac gaaggcgatc aatcgatctc
      901 ggggacggta ttgatcacct ataaaaataa cagcccgcag aaccttccct ttgtgtggct
      961 ccagttggat cagaatattt acgcgcaagg ttcgcgtggc gaagaggtaa ccgagatctc
     1021 cggcgggcgc tgggcaaacc ggaattcatt tgtggggggc gacgagttga agtccgtgga
     1081 actgatcaat cccgccactg gaaaggccat gccggccgat tacctggtta gcgatacgcg
     1141 gatgcagatc aaactgccgg ccgagctaaa ggctggtggc ggcatcctga agatccgcat
     1201 ccaatatagt ttcaagattc ccgagtatgg cacggaccgc atggggcgga tgcaggcgaa
     1261 gaacggcgca tggatgtatg acatcgcgca gtggtatccg cggatgtgcg tatatgataa
     1321 tgtcctcggc tggaacacgc agccctacct gggtcagggc gagttctatc tggaatatgg
     1381 ggatatcgat tacagcgtca atgtgccggg caacatgatc gtcggtggtt cgggcgaatt
     1441 gttgaatccg gcggaggtgc tcaccgccaa agagcaacgg cgtctggcgg aagcaaagaa
     1501 cagcgataaa acggtggcga tccgcgggaa ggacgaagtg gacgatcctg cctccagacc
     1561 ggctcaaaac aggctcacct ggaagttcag gtgtaccaat acccgggacg tcgcgtgggg
     1621 agcctcgtcg gcatacgtct gggatgctgc gcggatcaac ctgcccagcg gaaaaaaggc
     1681 gctggcggag tcggtgtatc cggtggaagt atcgagcgat tcggcctggg gccggtcaac
     1741 ggagtatgta aagggctgca tcgaattcta ttcaaaatat ctttatgaat tcagctatcc
     1801 ctcggctacg aatgtggccg gcatcgtcgg cggaatggaa tacccgggta tcgtgttttg
     1861 cagttcgagg gcccgcggga ggggtctttg gggagtgacc cggcatgagt tcggtcataa
     1921 ctggttcccg atgatcgtcg gcagcaatga aaggaaatac ccgtggatgg acgagggctt
     1981 caatacattt atcaacacca tcgccgactc cgctttcaac aacggtgaat actggtcaaa
     2041 ggata
//
LOCUS       DATGKE010000486         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_527771, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000486 DATGKE010000000
VERSION     DATGKE010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527771"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..2297
                     /locus_tag="VKU83_08405"
     CDS             69..2297
                     /locus_tag="VKU83_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLI93616.1"
                     /translation="MKKFTILLLHLACLAAVCRAQTASKVEALLRRMTLDEKIGQMTQ
                     VTLGVVSAPQDGILDAAALAKAVEKYKVGSILNVTNHALTVDQWRHVITQIQDDAMKT
                     RLKIPVIYGLDGIHGQTYTLDATLFPQNIAMAATRNPELAAAVTKVAAKELRASGVRW
                     NFAPVLDCGRQPLWSRFAETYGEDVYIGTTMGAAVVKAYEEDGLRNPTAVASCMKHYL
                     GYSASRTGKDRTPIYMPEIEMREYYLPQFRAAVRAGASTIMVNSSEINGTPVHASHYL
                     LTDVLRKELGFEGVVVTDWEDIIRLHTRHDVAATPRAAVVLAINAGIDMSMVPGDFSF
                     FDLLKEAVQKGEVPMSRIDDAVRRILTLKERLGLFDNPYPEEAARANFDRPEYQALAL
                     QAAHEAITLLKNDDNTLPLSRSTHLLVAGPSARSISALNGCWSYTWQGADERWYPAGS
                     KTILDALKDKFGAANVITTTEKGFDNPANFDTTALKNAAAGADAIVLCLGENAYAESP
                     GNTRDLALPDNQLAMARAAASTGKPVILLLTEGRPRFITAIAPLMKGIVMAYWSGRKT
                     AEAVSDILAGDYNPDGLLPFSYPKSMGEVVLYDRKPTEDVREVFNDDRGAGYDPLFPF
                     GWGLSYTTFEYSDLRLSSGKLDAHGKLTVSITVTNTGGRDGQHTVELYTHEHYASITP
                     NMRRLRAFKKIMLKAGESKPVTFTLDKNDLAFVNAALHTVTEPGDFDVIIGDKKATFT
                     YQ"
     gene            complement(2326..>2714)
                     /locus_tag="VKU83_08410"
     CDS             complement(2326..>2714)
                     /locus_tag="VKU83_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93617.1"
                     /translation="DFEFNHVINNHKLYDIIMRHTDPALVTQQLDMGNLYNGGAVAAG
                     IIKEYPGRYELLHVKDEIKSPAGSQEKYESCILGQGIVNTREVCDLAKKLDSPRYYII
                     EQESYQGKDPLDCCKQDLAIMKQWGY"
BASE COUNT          615 a          788 c          728 g          583 t
ORIGIN      
        1 tattccttaa accacttacc tttaatcttc acaattcgtt atcaccttca cctttccctg
       61 acagatttat gaaaaaattc acgattctgc tgctgcatct cgcatgtctg gctgccgttt
      121 gccgcgcaca gaccgcctca aaagttgaag ccctgctccg gcgcatgacc ctcgatgaaa
      181 agatcgggca gatgacccag gtcacccttg gcgtcgtgtc cgcgccgcag gacgggatac
      241 tggatgctgc ggcacttgcc aaagccgtag agaaatataa ggtgggctcg atcctcaatg
      301 ttaccaacca tgccctgaca gttgatcagt ggcgccatgt gattacccag atccaggacg
      361 atgcgatgaa gacgcgcctg aagatacccg tcatctacgg tttggacggg attcacggtc
      421 aaacctatac gctcgatgcc acgctctttc cacaaaatat cgcgatggct gcgacccgca
      481 acccggaact ggcagccgcg gtcacgaaag tagcggcaaa agaacttcgc gcttcgggtg
      541 tccggtggaa cttcgcccca gtcctggact gcggccgaca gcctttatgg tcacggtttg
      601 ccgaaacata cggagaagac gtctatatcg gtactacgat gggcgccgcc gtcgtcaagg
      661 catatgaaga agacgggtta aggaacccca cagcagtcgc tagctgcatg aaacactatc
      721 tcggctattc tgcttcccgg acgggtaagg accgaacgcc tatctacatg ccggaaatcg
      781 aaatgcgcga atattatctt ccgcaattca gggccgccgt ccgggcaggc gcctccacga
      841 tcatggtcaa ctcttccgag atcaacggca cgcctgtcca cgcgagccac tatctgctga
      901 cggacgtgct tcgaaaggaa ttggggtttg aaggggtggt cgtaaccgac tgggaggata
      961 tcatccggct ccatacccgg catgatgtcg ccgctacgcc ccgggcggct gttgttctgg
     1021 cgatcaatgc cggcatcgac atgagcatgg tgccgggcga tttctctttt tttgacctgc
     1081 tcaaagaagc ggttcaaaag ggggaagtgc ctatgagccg gatcgacgat gccgtcaggc
     1141 ggatccttac cctcaaggaa cgccttggcc tgttcgacaa tccatatccc gaagaggccg
     1201 cgcgcgcgaa ctttgaccgg cccgaatatc aggcgcttgc tttacaggct gcccatgaag
     1261 ccatcaccct acttaaaaac gatgataaca cgctgcccct ttcccgttcg acccaccttc
     1321 tcgtggcggg cccttcggcc cgtagtatca gcgcactcaa cggttgctgg agctatacct
     1381 ggcagggcgc cgacgaacgg tggtatccgg ccggcagcaa gaccatcctg gacgcactga
     1441 aagataaatt cggcgccgcc aatgtgatca ccacaacgga gaagggcttc gataacccgg
     1501 ccaattttga taccaccgcc ctgaagaacg ccgcggcggg agccgacgcc atagtgctgt
     1561 gtcttggtga gaacgcttat gcggaaagcc cgggaaacac ccgggacctg gcccttcccg
     1621 ataaccagct ggcgatggcg cgcgcggctg cctccacagg caaacccgtc atcctcctgt
     1681 taaccgaagg cagaccgcgc ttcatcactg ctattgcccc gctgatgaaa gggatcgtga
     1741 tggcttactg gagcggccgc aagaccgctg aagcggtcag cgatatcctc gcgggggact
     1801 ataatcccga tggcctgctc ccgttctcgt atccaaagag catgggcgaa gtcgtgctat
     1861 atgaccggaa gcctaccgag gatgtccgcg aagtgttcaa tgacgatcgc ggcgccggat
     1921 atgacccatt gttccctttt ggttggggat tgagctatac cacctttgaa tacagcgatc
     1981 tccgcctgag cagcgggaag ctcgacgcgc atggcaaact gaccgtaagc attaccgtta
     2041 cgaacaccgg cggccgggac ggccagcaca cggtcgagct gtacacgcac gaacactatg
     2101 cgagcattac gccaaatatg cggcggcttc gcgccttcaa aaagatcatg ctgaaagcgg
     2161 gcgagtcaaa accggtgacc ttcacgctcg ataagaacga tcttgccttt gtgaacgcag
     2221 cattgcacac cgtcaccgag ccgggcgatt tcgacgtaat tattggcgat aaaaaggcga
     2281 cgttcaccta tcaataagcg gctgcgattt caccaatcgc gctgttcaat agccccactg
     2341 cttcatgatg gcaaggtcct gcttgcagca gtcgagcgga tccttgccct gataagactc
     2401 ctgttcaatg atataataac gggggctgtc caactttttg gcgaggtcgc aaacctcccg
     2461 ggtatttaca ataccttgtc ccaggatgca gctctcgtat ttttcctggg agcctgccgg
     2521 cgacttgatc tcatccttca cgtgaaggag ttcatacctg ccggggtatt ccttgattat
     2581 acccgctgcg acggcgcccc cgttataaag gtttcccatg tcgagttgct gggtcaccag
     2641 cgccggatcg gtatggcgca taataatatc gtagagtttg tgattgttga tcacgtggtt
     2701 gaattcaaag tcgt
//
LOCUS       DATGKE010000487         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1491200, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000487 DATGKE010000000
VERSION     DATGKE010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491200"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VKU83_08415"
     CDS             <1..300
                     /locus_tag="VKU83_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93618.1"
                     /translation="KTGQYELSFEDFMAVVAVSIGVEAGVMVTKSALTRIAEQLLARM
                     GERTAGRLLPILGAAIGGTTNYLFIKAIGASVKRVVSGSAPGSRRQFSFGSRIKA"
     gene            347..961
                     /locus_tag="VKU83_08420"
     CDS             347..961
                     /locus_tag="VKU83_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93619.1"
                     /translation="MYYLHGMGHFIRVLGIYDDKIRPEELENALADDGLVARLAPDRE
                     NWGLIEVESEDGALLTQIERNIVFPGCLAQAELDEFRALIRAHQPLSAVQWLDNYFDR
                     IKVIYAFRIMDAALLDDNYEIISTLRRAIWGKTGGLLQNDLEGFSNDEGYHILWQFPD
                     DITGDKYCAVLDNGTWVKFRMDLSDPFQRMAFWAGEVPTMAVKL"
     gene            complement(986..1921)
                     /locus_tag="VKU83_08425"
     CDS             complement(986..1921)
                     /locus_tag="VKU83_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93620.1"
                     /translation="MKPLSGISRIAALLLLHVSAAPAFGQGPVFGVYFNAGTIKYLPA
                     HDGAARPLVFHDWLAEGDRVLLQDNIAELILIDRDSNYIRLDGKGNYTVADLEKMPRN
                     HVADSITLRYFSLLWTEAPHSSPHSPATKEKTLSGVNGRQHSAANLMPATPFMVAPRH
                     GYSTSLDSLIFRWHRISWAQKYLLRIRNLAGEVRYDSVLVDTEAVVNFAGRMSPDTYY
                     WALDLVGASGRLQFGDSSHIALIDEAAVLPQLPPLPVDSLGGIATILRRIEQYEAFGC
                     LRHASNLFQQLTVDFPTDGALDKMYQEFLQRNYLR"
     gene            2135..>2555
                     /locus_tag="VKU83_08430"
     CDS             2135..>2555
                     /locus_tag="VKU83_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93621.1"
                     /translation="MRGRLSFCCLLLMSAASGQTYQGQVDSALLPVLNYHYQDEFTLD
                     KPTNRSAWDSQQGPHVSFGPTNDRYFRTEVPKLTETTTWEGCGWRGERLNAQIVVWSA
                     DTLQQVRFRPSALRAADGALISKGNITVNMVRYVLGNY"
BASE COUNT          562 a          634 c          816 g          543 t
ORIGIN      
        1 aaaacaggcc aatatgaact gagtttcgag gacttcatgg cggtggtggc cgtctccatc
       61 ggcgttgagg ccggcgtgat ggtcacgaag tctgcgttga cccggatcgc ggagcagctt
      121 ctggcccgaa tgggagagcg gacggcggga aggctattgc ccatcctcgg ggcagccatc
      181 gggggaacga ccaattacct tttcatcaag gcgattggcg cctcggtaaa gcgggtcgtc
      241 tcgggatcgg cacccgggtc gagacgccaa ttcagcttcg gctcacggat aaaagcatga
      301 gtggctcgca tcccgacgcc gcgggtgatt tcggcggcga ggtgaaatgt actaccttca
      361 tgggatgggc cattttattc gcgtgcttgg gatttatgac gacaaaatcc ggccggagga
      421 gctcgaaaac gcactggccg atgacggcct tgttgcccgg cttgctccgg atcgtgagaa
      481 ctggggcttg attgaggtgg aatcggagga tggcgctttg ctcactcaga tcgaaaggaa
      541 tattgtattc cccggctgcc tcgcacaagc cgagctggat gagttccggg cgttaatccg
      601 ggcgcatcaa ccgctgtctg ccgtgcaatg gctggataat tatttcgacc ggattaaggt
      661 catttacgca ttccggatca tggatgcggc gctgctcgat gacaattacg agatcatcag
      721 caccctgcgc cgggcgatct gggggaagac cggcggactg ctacaaaacg atctcgaagg
      781 cttttccaac gatgaaggct atcatatcct ctggcaattt cccgatgaca tcaccggtga
      841 caaatattgt gcggtgctgg acaacggaac atgggtaaag tttcggatgg acctgtccga
      901 cccattccaa aggatggctt tttgggccgg cgaagtgccg accatggccg tcaaactcta
      961 actcgctatc accggaggcc gcttattacc tgaggtagtt acgctgcaga aattcctggt
     1021 acatcttatc cagcgctccg tcagttggga aatcgaccgt gagctgttgg aacaaattgg
     1081 aagcatgccg caggcagcca aaagcctcat attgctcaat tcttcggaga atcgtggcaa
     1141 tgccgccaag cgaatctacc gggagcggag gtagctgtgg caagaccgcc gcttcgtcga
     1201 tcagcgcgat atggctggag tcgccgaatt gaagtcgccc cgacgcgccg accagatcga
     1261 gcgcccagta ataggtgtca ggggacatgc ggcccgcaaa attgacgacg gcctcggtgt
     1321 cgacaagcac gctatcgtat cgcacctcgc cggcgaggtt tctgatccgc agcaaatact
     1381 tctgcgccca gctgatccga tgccaacgaa agatcaggct gtccagtgac gtgctgtacc
     1441 cgtgccgggg cgccaccatg aaaggtgtcg cgggcatgag gttggcggcg gagtgttgac
     1501 ggccgttgac gccggataag gtcttttctt ttgttgccgg gctgtgcggg gaggagtgcg
     1561 gagcttcggt ccacaaaagc gaaaaatagc gaagcgtgat gctgtcggca acgtggttgc
     1621 ggggcatttt ttcgaggtcg gcaacggtgt aatttccctt gccgtcaaga cggatataat
     1681 tgctgtcgcg atcaatgagt attaattcgg caatattgtc ctgaagcagt accctgtctc
     1741 cttcagcgag ccagtcgtga aagacgagtg gcctggcggc gccgtcatgg gcgggtaagt
     1801 atttgatggt gccggcattg aaataaacgc cgaacacggg gccctggccg aaggcgggcg
     1861 cggcggaaac atggagtaga agaagggcag cgattctact aatgccggat aatggcttca
     1921 tagtcaaata tagcgatgat cgggccgcga ggggggatag cacccggaaa acttttactg
     1981 ctggcgcggg ttgtcggcgg ttgcccggcg ccggcggggg gtgtaggtca gggttaattg
     2041 ggccgatttc ggcccaatta aggtaaaggg tggggcggca gggtgaacgg cgggcgattc
     2101 atgtataatg caagtggata cgtaaattgc ggggatgaga ggaaggttat cgttttgttg
     2161 cctgctatta atgagcgcgg cctctggcca aacatatcaa ggccaggtcg actcggccct
     2221 tctaccggtc ctgaactatc attaccagga tgaatttacg ttagacaaac ctacgaatcg
     2281 ctccgcctgg gattcccaac aagggccgca cgtaagtttc ggcccgacga acgaccggta
     2341 tttccggacg gaggtaccga agctaacgga gaccacgaca tgggaagggt gcggctggcg
     2401 cggggagcgg ttgaatgcgc agatcgtggt ttggtcggcc gacacgctgc aacaggtgcg
     2461 gtttaggccg agtgcgctga gagcggcgga tggcgccttg atcagtaagg gcaatatcac
     2521 cgtgaacatg gttcgctacg ttttaggaaa ttatc
//
LOCUS       DATGKE010000488         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_735133, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000488 DATGKE010000000
VERSION     DATGKE010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735133"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VKU83_08435"
     CDS             <1..803
                     /locus_tag="VKU83_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLI93622.1"
                     /translation="QAGANVWFEGMKVGTVKRLSFDGRRHVRVEMDVDRSAGQYIPMN
                     ANASVGLDGLIGNRIVVLRGRGENAPPIADGAVLPGGRQEGSDMLGTLGESSRNLVKI
                     TEDLREVARKIDTGGGTVGRLINDGSLAAGMQRLVNRMNQAAVDLQQSSADIRTFSAG
                     LNNKSGLTARLVNDTTLFSSLLATAATINEAAGRASAAMDTAYRALGALNDTSRPVGM
                     LLGDRETAADLRATIANLRKSSVELSEDLEALQHNFLLRGFFRKKQKK"
     gene            complement(838..1620)
                     /locus_tag="VKU83_08440"
     CDS             complement(838..1620)
                     /locus_tag="VKU83_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93623.1"
                     /translation="MKNIIATVDFTETSNNAARYAADLAFAIGAELHLVHVLDYKSIY
                     PEIPVPDYVLDEVRNNGFGSLDKLVAELKARTGQKISIATDLETGPVERQIREFCRWK
                     KPFLVVRGAARGETPEIGRLPFPLLTIPAGAVFHSVRNIVIACDEEEINNGMPVPMEF
                     LLELRNQLQAHFDVLHVASRREESATISFQQWRATLTEKFPEIHFVSAPTVDQGVKEY
                     LDHHSVDWLVVFPKKGGLFGFRKSASAKIVLHCPVPVMSYKE"
     gene            complement(1642..2670)
                     /locus_tag="VKU83_08445"
     CDS             complement(1642..2670)
                     /locus_tag="VKU83_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLI93624.1"
                     /translation="MPDAIKPYNNIRLGALVLSGLLLFIGTLYIIGKNEHFFGSNFEL
                     KARFTNVAGLITGDNIRFAGIQAGTVKRINLLDDTTIEVTFLIDNSLRPFIRKNALAT
                     IGNEGLMGNKVINITPVSGPSEVAHPGDLLAVAPLSGTDEMLAKLAKTNNNIELISED
                     LLFTIRRFNRSAGLWAILDDAGLARRVHASLEEIDQASANASAITADIRSIIADTKKG
                     KGTIGSLLRDTALVFNLTQAAERIHSASVHADELTATLEATMQDLQHSIAEGQGAAHS
                     LLKDPAMTEKINASVENIRLGTAAFNEDMQALQHNFLLRGYFRRQEKKAKIKPAQTAA
                     TTAPGIPK"
BASE COUNT          567 a          702 c          806 g          622 t
ORIGIN      
        1 tgcaggccgg ggctaatgta tggtttgaag gaatgaaggt cggcaccgtg aaacgccttt
       61 catttgacgg ccggcgccat gtcagggtag agatggatgt cgacaggtca gccggtcagt
      121 atatccccat gaatgcgaac gccagtgtcg gccttgatgg acttatcggc aatcgaatag
      181 tggttctccg cggcagaggc gaaaatgcgc ctcctatcgc ggatggagct gtcctgccgg
      241 gcggcaggca ggagggatcg gatatgctgg gcacccttgg ggagagcagc aggaaccttg
      301 tgaagatcac cgaggacttg cgggaagtgg ccaggaagat cgataccggc ggtgggaccg
      361 tgggcaggct gatcaacgat ggatcgcttg cggcaggaat gcaacggttg gttaaccgga
      421 tgaaccaggc ggccgttgat ctccagcagt catcggcgga cattcgtacc ttttcagcag
      481 ggctgaacaa taagagcggg ctgacggcca gactggtaaa tgataccaca ctgttttcat
      541 ccttgcttgc gacggcagcg actatcaatg aggcggcagg cagagcctcg gcggccatgg
      601 atacggctta tcgggcactt ggcgcattaa atgatacctc acggcctgtt ggcatgctgc
      661 ttggagaccg ggaaactgcc gcagacctgc gggcgaccat tgccaacctg cgaaaaagca
      721 gtgtcgagct atccgaggac ctggaggcgc tgcaacataa tttcctcttg cggggcttct
      781 tccggaaaaa acagaaaaag taaggcagcg caagcccggg cggcatggcc tgcccggtca
      841 ttctttgtag ctcatcaccg gcacggggca atgtagaacg atctttgctg atgcgctttt
      901 tcgaaaacca aaaagtccgc cttttttcgg gaaaacgacg agccagtcga ccgagtggtg
      961 atccaggtat tccttgacgc cctggtcaac cgtaggagcg ctgacaaaat ggatttcggg
     1021 gaatttctcc gtcaacgtcg cgcgccactg ctgaaaggag atggtagcgc tttcttcacg
     1081 ccgggatgct acatgcaaca cgtcgaagtg cgcttgtaac tggttcctca gttccagcag
     1141 aaattccatg ggtaccggca ttccattgtt gatctcttcc tcatcacagg cgataacgat
     1201 gttccggaca ctatggaaga cagcgcccgc aggtatcgtc aggagcggga aaggcagtcg
     1261 gccgatctcc ggtgtttctc cgcgggcagc gccgcggacg acaagaaaag gctttttcca
     1321 gcggcagaac tccctgatct gcctttccac gggtcccgtt tccaggtcgg tggcaatgct
     1381 gatcttctgg cctgtcctcg ctttgagctc ggcaaccagt ttatccaggc tgccaaaccc
     1441 gttattccgg acctcatcca gcacataatc gggcacgggg atttccgggt atatcgattt
     1501 ataatccagc acgtgcacga gatgcagctc ggcgccgata gcgaaggcca ggtccgcagc
     1561 atagcgcgcg gcattattcg atgtttctgt aaaatcgacg gtggcaataa tatttttcat
     1621 atcgcgctgt tttttaggtg atcatttcgg aatgccaggc gccgtcgtcg cagcggtctg
     1681 tgctggcttt attttcgctt ttttctcctg ccgccggaaa tagccgcgga gcaggaaatt
     1741 gtgctgcagc gcttgcatgt cttcattgaa cgctgcggtt ccgaggcgga tattctccac
     1801 gctggcgtta attttttcgg tcatggccgg atctttcaac aagctgtggg cggctccctg
     1861 accttccgca atggaatgtt gcagatcctg catcgtagct tccagcgtgg cggtcagctc
     1921 atcggcgtgg acgctggccg aatggatcct ttccgcggcc tgggtaaggt tgaaaacaag
     1981 agcggtatcc cgcagcaggc tcccgatggt gccttttccc tttttggtgt cggcgataat
     2041 ggacctgatg tcggcggtga tcgcgctggc gttggcgctg gcctggtcga tctcttccag
     2101 tgaggcatgc acccgccggg cgagccccgc gtcgtcgagg atcgcccaaa gaccggcact
     2161 ccggttaaac cggcgaatcg tgaatagtaa gtcttccgaa atcagctcaa tattgttgtt
     2221 tgttttcgcg agcttggcca gcatttcatc ggtccccgaa aggggggcga ccgcgagcag
     2281 gtcgccggga tgcgcgactt ccgatggacc gctaaccggt gtgatgttga taaccttgtt
     2341 gcccatcaag ccttcattgc cgatcgtggc gagggcattc ttccggatga agggccgcag
     2401 ggaattgtcg atcagaaacg tgacctcgat ggtggtgtca tcgagcaggt tgatccgctt
     2461 gactgttccc gcctggatgc cggcaaagcg gatgttgtcg ccggtgatca ggccggcgac
     2521 attcgtaaat cgcgccttca gctcaaaatt ggatccaaag aaatgttcgt tcttgccgat
     2581 gatatacagg gttccgatga acagcaggag cccgcttaat acaagggcgc cgaggcggat
     2641 attattgtat ggtttgattg cgtcgggcat attcaaatcg agttattcaa aaaattg
//
LOCUS       DATGKE010000489         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_962778, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000489 DATGKE010000000
VERSION     DATGKE010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962778"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VKU83_08450"
     CDS             <1..1653
                     /locus_tag="VKU83_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93625.1"
                     /translation="GTVFAQSVEQGKQFLYYHRYKSAKDVFDKILAANPNNIEAIYQD
                     GQAMIALKDSAAAQQLYSKALQTNGNAPLLLVGMGGVELRFGKPQDAKAHFEEAISLT
                     KSKDVPIFNAIAANNIEAAQGDAQYAIEKLTQATNVRHFNDPETYLLMGNAYRKLIDG
                     GNAVQAYQKALSMDPKLAAAEYGIGKIYLTQNNVDYFLPAFKQAVQMDPLYAPAWYEL
                     YYYYYFHWNTDKDNAKDALDHYIAASDPGPDVEYTKIDFKFVNGDFNGAKTDAQGIIS
                     QLGDKVEPRMYKLIALCCDTLGDAACAQQNIATYFQKVDTANIIPTDYVERAMIEGKS
                     TDSTTREKAFDDYGLAIRKDTLQANRDKYLADATALANKLNDKPALAKIARIVYMGLK
                     SPTNSDLFKWGMANYSAGNYKTADSIFCGMYESKYPDEIYGYLWCARALQAQDDSLSS
                     QGIAMPAWDKLAQVARAIDSTAKAAGSADSTKWKQSALNAYFTLASYYNDTKKDKAMA
                     IEYLRKILEIDPTNETANKFYKILTAPPRREPARKPAGTRQR"
     gene            1822..2235
                     /locus_tag="VKU83_08455"
     CDS             1822..2235
                     /locus_tag="VKU83_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLI93626.1"
                     /translation="MDIFLLQANTLATDSNLNNSVSYFPIGVQFIFAIAFVGLVLAVT
                     HLLGPRRRTSEKLQNFESGIEAVGNARTPMAIKYFLVAILFVLFDVEVIFFYPYAVNF
                     RELGWSGFGEVLLFVALFLAGFVYILKKGALQWED"
     gene            2248..>2533
                     /locus_tag="VKU83_08460"
     CDS             2248..>2533
                     /locus_tag="VKU83_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93627.1"
                     /translation="MARPVRFNIHPIQADIKQNISYANAPDGYPGEGFFATQLSSVVG
                     LARKNSLWPMPFATSCCGIEFMATMASHYDLARFGAERLAFTPRQCDLLMV"
BASE COUNT          621 a          644 c          709 g          559 t
ORIGIN      
        1 ggcaccgtgt ttgcgcaatc ggtggagcag ggcaaacaat tcctgtatta tcatcgctat
       61 aagagcgcga aggatgtctt tgacaagatc ctggccgcta atccaaacaa cattgaggct
      121 atctaccagg atggccaggc catgattgcg ctgaaggatt cggccgctgc ccaacaactg
      181 tattcgaagg cattgcaaac taacgggaat gcaccgttat tgctcgtggg aatgggcggc
      241 gtcgaactac gattcggaaa gccgcaggat gccaaggctc attttgagga agcgatcagc
      301 ctgaccaagt cgaaggatgt gccgatattc aatgcgatcg cggcgaacaa tattgaggca
      361 gcgcagggcg atgcgcaata tgcgatcgaa aagctgaccc aggcaacgaa cgtgagacac
      421 tttaacgacc cggagactta tctgttgatg gggaatgcat accggaagct gatcgacggg
      481 gggaatgcgg tgcaggccta tcagaaagcg ctgtccatgg accccaagct ggctgccgcc
      541 gagtatggga tcggcaagat ctacctgacg caaaacaacg tggattattt cctgccggcg
      601 ttcaagcagg cggtgcagat ggatccgtta tatgctccgg cctggtatga actatactat
      661 tattattatt tccactggaa cacggacaag gacaacgcca aggacgcgct tgaccactat
      721 attgccgcct cggatcctgg cccggatgtc gaatacacga agatcgactt caagttcgtt
      781 aacggcgatt tcaacggggc gaagacagat gcgcagggta tcattagcca gttaggggac
      841 aaggtcgagc cgcggatgta caagctgatc gcgctttgtt gcgatacgct tggggatgct
      901 gcctgcgccc agcagaacat tgcaacctat ttccagaaag tggacacggc caatattatt
      961 cctaccgact acgtggagcg ggccatgatc gaaggcaagt cgacggattc caccacgcgg
     1021 gaaaaagctt tcgatgatta tggactggcc atccggaagg atacgctaca ggctaacagg
     1081 gacaagtacc tcgcggacgc cacggctctt gccaacaagt tgaacgataa gccggccctg
     1141 gcaaaaatcg cgcggatcgt gtatatgggg ctgaagagcc cgacaaattc cgacctgttc
     1201 aaatggggca tggcgaatta ctcggctggg aattataaaa cggcggatag catcttttgc
     1261 gggatgtatg agtcgaaata cccggacgag atctatggat atctttggtg cgcccgcgcc
     1321 ctgcaggccc aggatgacag cctgagcagc cagggcattg cgatgccggc atgggacaag
     1381 ctcgcgcagg tcgcccgcgc catcgattcg acggcaaagg cagccggaag cgcggacagc
     1441 acgaagtgga agcagtcggc gctgaatgcc tattttacgc tcgcttccta ttataatgat
     1501 accaagaagg acaaggcgat ggcgattgag tatcttcgca agatcctgga gattgatccc
     1561 accaacgaga cggcaaacaa gttctacaag atcctgactg ctccgccccg ccgcgagccc
     1621 gcgaggaagc cggccgggac gagacagcga tagaagtaat tgaataaaaa gggactgtga
     1681 ccggcccggc gaagccgggc cggtctcttt attttaggtt tttgttggaa tttcagtggg
     1741 ctggcgtaaa tttgcggggc cggacgaagc ccggccggcg ccggaaggcg cagacatcaa
     1801 agcttaagaa atccctttat aatggacatc tttctgctac aggccaatac ccttgcaacc
     1861 gattctaatc taaataattc tgtcagttat ttccccatcg gtgtccaatt catcttcgct
     1921 atcgcatttg tcggactggt gctggccgtc acccatttgc tcggcccgag gcgcagaacg
     1981 tccgaaaagc ttcaaaactt tgaaagcggc atcgaagcgg tcggcaatgc ccggacgccc
     2041 atggccatta aatacttcct ggtagcgatc cttttcgtcc tgttcgacgt cgaagtaatc
     2101 tttttttatc cctacgccgt taatttccgg gaactgggct ggtccggttt tggcgaagtt
     2161 ttattgtttg tagcattgtt ccttgctggg ttcgtttata tcctcaaaaa gggcgcgctc
     2221 cagtgggaag actaaatatt caatacaatg gcacgtcctg taaggttcaa tattcatccc
     2281 attcaagccg atatcaagca aaatatcagc tacgccaatg ctcccgacgg ctaccctggg
     2341 gaaggttttt ttgccacaca gctcagcagt gtcgtcgggc tggcgcgaaa gaattccttg
     2401 tggccgatgc cctttgcgac ttcctgttgc gggatagagt tcatggcaac gatggcctcg
     2461 cactatgacc tggcgcgatt tggcgcggaa cggctggctt ttaccccgcg gcagtgcgat
     2521 ctgctgatgg tga
//
LOCUS       DATGKE010000490         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1286436, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000490 DATGKE010000000
VERSION     DATGKE010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286436"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..492
                     /gene="ssrA"
                     /locus_tag="VKU83_08465"
     tmRNA           119..492
                     /gene="ssrA"
                     /locus_tag="VKU83_08465"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            636..1829
                     /locus_tag="VKU83_08470"
     CDS             636..1829
                     /locus_tag="VKU83_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLI93628.1"
                     /translation="MPTVTVRLDTRYRSKGDLYPILIRIEHKGRRKDVSVGHKIEKKY
                     WLDGEVSRKHIEAVQINNAIDNKKAEIKRYLADCDLHGKPIHLDLIGSGRTSYSFTGY
                     LRQRSAQYLAAGKSSIAEKGKIVMSQKVDRYVKELEAVAGRQVFFEDVTLDFLRRLDQ
                     YLIECENIENTRHQKFKRLGEFYAQAIIEGKVSGSNPFDGYKIPKRDAKKEKLSPAEI
                     AAAESLSLKPGAVNDARNLFLCSFYTKGQRFETCITLRRAQIADGRIMIITNKGKDHM
                     SVKIHARLQAIIDQYPGDGELLFPFLDELPTDPKTYLSAIGSQNAIVNRNLKTVAALA
                     KIKKRLTFHIARHSFADQLSNLTDSVSIVQAALGHAAEKTTRLYLNALKDERLDPEME
                     KLYGK"
     gene            complement(1831..>2216)
                     /locus_tag="VKU83_08475"
     CDS             complement(1831..>2216)
                     /locus_tag="VKU83_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HLI93629.1"
                     /translation="SNSSWIAIMSASQADPTAGVIPLGKAYGGLNLQVKAIKKLGNKK
                     RGFRYYIKVGGGNIVNYQVELEDAIAVGEIVGTNVSVPIQMAAIPNNASSPADELKKL
                     KQLLDAGAITQQEYDSTKKKILAKM"
BASE COUNT          568 a          579 c          549 g          520 t
ORIGIN      
        1 gcgatacttg cggcgatagc gtatgttcgg gtccggggct tgggggttgc gggataattt
       61 tgtatatttg ctgttcgcct gactgcggcc ggtggctgat cggccgtagc gggaaaatgg
      121 ggctgccttg gttttgacag catagatctt tgaaggtgta agcatgccgt gcgttggata
      181 attagcacgt taatctgaat attcaaaact caaatggcaa tactcagtat gccatggctg
      241 cctaatcagt gattagatag tcatttgggc cgcgctggca ggtttgcctg tagccaagct
      301 gcgccgccga ctcaaaacaa ctacaggatg ctgcaacaga aggctgtgac tgttgcttaa
      361 gaccatacag ctaaggagga agttggtttg tccggtcccg gccgcctccc gacaagcaat
      421 gccggaataa gcatgtagaa agcttttggc gattatgttt ggacaggggt tcgactcccc
      481 tcagctccac accaaaaccc ggtattttgc cgggtttttc tttgcccacg cgggtttcca
      541 gcagattgag catagccccg gaaagccccg aaaacacccg gaagtacccc attttcgtag
      601 caaaaaagta gctacattcg tagcaaaacc aaaacatgcc caccgtcacc gtccgcctcg
      661 acacgcgcta tcgcagcaaa ggcgaccttt atcccatcct catccgcatc gagcacaaag
      721 ggcgtcgcaa agacgtttcc gtaggccata agatcgaaaa gaagtactgg ctcgacggcg
      781 aggtttcccg aaagcatata gaagccgttc agatcaataa tgcaattgat aataagaagg
      841 ccgaaataaa gcgttacctt gccgactgcg acctccatgg caaacccatt catcttgatc
      901 taatcggctc cggacgcact tcctatagct ttacaggcta tctccggcag cgaagcgcac
      961 aatacctggc ggcgggaaag tccagcattg ccgaaaaggg caagatcgta atgtcccaga
     1021 aggtggatcg gtatgttaaa gaactggagg ccgtcgccgg ccggcaggta tttttcgagg
     1081 acgttaccct ggacttcctg cgccggctgg accaatatct tatcgagtgc gaaaatatag
     1141 agaatactcg ccaccaaaaa ttcaaacggc tgggcgagtt ctatgcgcag gcgatcatag
     1201 agggaaaggt aagcggctcc aatcccttcg atggttataa gataccgaag cgcgatgcga
     1261 aaaaggaaaa gctctcgcct gccgagatcg cggcggccga atccctatcg ctaaagccgg
     1321 gagcggtgaa tgacgcccgc aacctattcc tgtgttcgtt ctataccaag ggccagcggt
     1381 tcgagacctg catcaccctg cgccgggcac aaatagccga cggccggatc atgatcatca
     1441 ccaacaaagg caaggatcac atgagcgtga agatccacgc gcgactacag gcgatcattg
     1501 accaatatcc cggggacggc gaactgctct ttccttttct ggatgagctt cctaccgatc
     1561 ccaaaaccta tctatcggcc atcggtagcc agaacgctat tgtcaatcgc aatctgaaaa
     1621 ccgtcgccgc actggcgaag attaaaaagc ggcttacctt tcatatcgcc cgtcattcct
     1681 tcgccgatca gctgagcaac ctaaccgaca gtgtgagcat cgtacaagca gccctcggtc
     1741 acgccgctga gaaaacgaca agactatatt tgaacgcatt gaaagacgaa cggctcgacc
     1801 ccgagatgga gaagctgtac gggaagtgat ttacattttg gctaaaatct tcttcttggt
     1861 ggaatcatat tcctgctggg taatcgctcc tgcatcaaga agttgtttca gttttttcaa
     1921 ttcgtcggca ggacttgatg cattattggg tattgcggcc atttgaatcg gaacgcttac
     1981 attagtccca acaatttcgc ctaccgctat tgcatcctca agttcaactt gataattaac
     2041 tatatttccg cccccaactt taatgtaata tctgaagcct ctttttttat tgccaagctt
     2101 ttttatcgcc ttaacttgaa gattgagacc gccataggct ttgcctagcg gaataacccc
     2161 ggctgtcgga tcggcctgcg atgcgctcat aatggcaatc cagctgctgt tcgatg
//
LOCUS       DATGKE010000491         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1504087, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000491 DATGKE010000000
VERSION     DATGKE010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504087"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VKU83_08480"
     CDS             complement(<1..1161)
                     /locus_tag="VKU83_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93630.1"
                     /translation="MFKLWATILKDVRILRRDRVGLTFMFIMPVLLVLIVTGLQSGSF
                     DLASRNKIPVLVCNNDTGASGIGFMRALDTMGMLAVRRIPPRETQAAIRERLHQKQAL
                     LAVVLPVDFSAKLTARTNRLAAKALKSFGLQDSSAEMAGDPSLTLYYHPVLAEAARRS
                     VDGAIYGALQLIESRQLLRKIYFSINEKELPDSLERDLLANGTSISEVAVSLDGARAS
                     PTVSQHNVPAWAIFAMFFVVVSLGSGIVREKLNGSFIRLKTMPTSYSVAILSKQITYL
                     AVTMVQTIVIFAVGIWVLPLVGLPGLELPADGWGLVLLSLLCGACAVSYAMCVGVFAG
                     TEEQANGFGAVSIVILAAIGGLMVPDFLMTGAFRTAMKFSPLHWCLDAYYGLF"
     gene            complement(1161..1919)
                     /locus_tag="VKU83_08485"
     CDS             complement(1161..1919)
                     /locus_tag="VKU83_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93631.1"
                     /translation="MDNLNPAPAIRIEGLGFHYPGQPGLFSQFHLEVEAGRRFGLFGP
                     NGAGKTTLMNLMTGLLTADSGSIRLFGQPMRLHDRRLHALLGFVPQDLSFYQELSPVE
                     NLAFFGAWAGMRTPAIRKRTVELLDILGLSAVRDKKVRQFSGGMKRRVNLAIGVIHEP
                     RLLFLDEPTVGVDVQSRHAIIAYLKQLNAGGTTLIYTSHQLAEAEELCDRVALIDQGR
                     IIAEEDLDTLLSTHRADGLEGLFISLTGKNFRDL"
     gene            complement(1907..>2325)
                     /locus_tag="VKU83_08490"
     CDS             complement(1907..>2325)
                     /locus_tag="VKU83_08490"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HLI93632.1"
                     /translation="DMLYIPGPIAGVSGIRYTARRIKKWRDSLGLKQAGLYLAQLVRM
                     QEEIGTGGGGFRYIYAAFLQEAHAYCPNDILLSASASFTRAGDLWRDAAVHAAGIYKG
                     RLGSQGDFDRMGEFLLEIAEVEKASFTALSRIKWTI"
BASE COUNT          500 a          720 c          674 g          431 t
ORIGIN      
        1 aaacaggcca tagtaggcat ccaggcacca atgcaacggc gagaatttca tcgcggtccg
       61 aaatgccccc gtcatcaaaa agtcgggcac catcaagccg ccgattgcgg cgaggatcac
      121 gatcgacacc gccccaaacc cgttagcctg ttcttctgtt ccggcgaaga cgccgacaca
      181 catggcgtag ctgacggcgc aggcgccgca aagcagggac agcaggacga ggccccaccc
      241 gtccgccgga agttccagcc ccggcaggcc caccagtgga aggacccaga tgccgaccgc
      301 aaagatcacg atggtctgga ccatggtcac cgcgaggtag gtgatctgtt tggacaggat
      361 ggctaccgaa tagctggtag gcatggtctt cagccggata aaactgccgt tcagcttctc
      421 cctgacgatg ccgctgccaa gggacacgac gacgaaaaac atcgcgaaga tggcccaggc
      481 gggaacgtta tgttgtgaaa ccgtgggcga agcacgggct ccatccaggg aaaccgcaac
      541 ctcgctgatg gacgtcccat tcgccagaag atccctttca agggagtccg gcagttcttt
      601 ttcgttgatc gaaaaataaa ttttccgcag gagctgccgg ctttcgatga gctgcagtgc
      661 gccgtagatc gccccgtcga ccgatcggcg ggccgcctcc gccaggacgg gatgataata
      721 caaggtgagg gaggggtcgc cggccatttc ggcggagctg tcctggagtc cgaaactttt
      781 caacgccttc gcggcaagcc ggttggttcg cgccgtcagt tttgccgaga aatccaccgg
      841 caggaccacc gccagcagtg cttgtttttg gtggaggcgt tcccggatcg ccgcctgcgt
      901 ctcgcggggc ggaatccgcc tgaccgccaa cattcccatg gtatccagcg cccgcatgaa
      961 accgatgccg gacgcgccgg tgtcattgtt gcaaaccagc accgggattt tgttccggga
     1021 ggccaagtcg aaactgccgc tttgcaatcc ggtcacgatc aggaccagca aaaccggcat
     1081 gatgaacatg aacgtcaggc ctaccctgtc gcgccgcagt atcctgacat ctttcaatat
     1141 ggtcgcccaa agtttgaaca ttacaggtcg cgaaaatttt ttccagtcag tgaaataaaa
     1201 agtccttcca gtccgtccgc ccgatgtgtg gaaagcaggg tatccaggtc ttcctccgcg
     1261 atgatccgtc cctggtcgat cagggccacc cggtcgcaaa gctcttccgc ttccgccaac
     1321 tggtgggacg tgtagatcag ggtcgtcccg ccggcattca attgcttaag gtaagcgatg
     1381 atcgcatgcc ggctctggac atccacccct accgtgggtt catccaggaa aagcagccgc
     1441 ggctcgtgga taacgccaat cgccaggttc acccgccgtt tcatgccccc ggaaaattgc
     1501 cggacctttt tatcgcgcac ggccgaaagt ccgaggatat ccagcagttc aactgtcctt
     1561 ttgcggatcg cgggcgtgcg catccctgcc caggccccga aaaaagcaag gttctcgacc
     1621 gggctcaatt cctggtaaaa cgacaggtcc tgcggaacga acccaaggag cgcgtgaagg
     1681 cgccggtcgt gcagacgcat cggctggccg aagagccgga tcgagcccga gtcggcggtc
     1741 agcagccccg tcatcaggtt catgagcgtc gttttgccgg caccgttagg cccaaagagg
     1801 ccgaaccgcc ggccggcctc gacttccaga tggaattggc taaaaagccc gggttggccg
     1861 gggtagtgaa agccaaggcc ctcgatccgg atggcggggg cggggttcaa attgtccatt
     1921 tgatcctgga taaggcggtg aaggatgctt tttccacttc ggctatttcg agcaggaact
     1981 cgcccatccg gtcaaagtct ccctggctgc ccaaccggcc tttgtagatc ccggccgcat
     2041 gaacggcggc atcccgccag agatcaccgg cccgcgtaaa ggacgcggac gccgacaaca
     2101 ggatgtcgtt ggggcagtag gcatgggctt cctgcaaaaa ggcggcataa atataccgga
     2161 agccaccgcc tcccgtcccg atctcctcct gcatccgcac cagctgcgcc aggtacagac
     2221 ccgcctgctt caaaccgagt gaatcccgcc acttttttat ccgccgggcc gtgtaccgga
     2281 tcccgctaac gcccgcgatc gggccgggga tatagagcat gtccc
//
LOCUS       DATGKE010000492         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1259785, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000492 DATGKE010000000
VERSION     DATGKE010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259785"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VKU83_08495"
     CDS             <1..1821
                     /locus_tag="VKU83_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLI93633.1"
                     /translation="TDHWKLDLPNAPYLFFMGVGDYAIIKDSYKGKEVAYYVEKAYAP
                     VARRIFGHTPEMMAFYSRITGVDFAWPKYDQIVGRDYVSGAMENTTCTLHGEQAQQDA
                     RELTDGNQWEDVIAHELFHHWFGDLVTCESWSNLTLNESFADFSEELWNEYKYGKDAG
                     DAVNYAGIQSYLQQPENADKDLVRFHYADKEDLFDQVTYPKGGRILNMLRNYVGDSAF
                     WKSLNLYLNTYKFQSAEAQELRLAFEQVTGEDLNWYWNQWYYGSGHPKLTIDYAYDDA
                     GGKATVIVNQTQDGGKIFKLPVAIDVYNGASKVRHKVWVQDKSDTFTFAYQNRPDLIN
                     FDGDKILLCEKTDNKTLDNYIHQYQYAGLYLDRREAIDFCSRHEDDPKAVELLKAALK
                     DRFYKLRDFTLSKLNFQQDGVKAAFEPLVARMAENDPSRVVKATAIEILGSLKNPAYE
                     ALFTKATNDSSYTVSGNALESLLAIDSVAALKIAKEFQREPAKGELAAAIAIVLIKNG
                     DESDFDAITNTFARMPVTQAKFEFLRPYLSLLANVKSLDHLKRGVDELLKFKVAIPAA
                     YRNQTDPFFDAALNSLATKKAAAGMQDQADYIKSAMEKQK"
     gene            complement(1842..2645)
                     /locus_tag="VKU83_08500"
     CDS             complement(1842..2645)
                     /locus_tag="VKU83_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLI93634.1"
                     /translation="MYKLFTIFLFSSCLAVASMKAQAQEIPPKPNPATPVNDYAHVMT
                     PDQIDALDRKLEAYDDSTGTQIVIVTVPDIGGAAIDDYGLKILRTWGVGSKKNNNGVV
                     IVADIKDRQINIAVGYGLEGAIPDITAKSIIDNEIVPNFRGGGNDNYYRGFDQGVDAL
                     IKAAAGQYHAEGGGHRRGGGNGIGILVIILIIIFLIIIRRGGGRGGGGYFGGGGWWPF
                     FLGTMVGRGFGGGGDRWGGGGGWGGGGGGGFGGGFGGGSGGGGGASGGW"
     gene            complement(2659..3117)
                     /locus_tag="VKU83_08505"
     CDS             complement(2659..3117)
                     /locus_tag="VKU83_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLI93635.1"
                     /translation="MHIPFFSKKKLFTAAEQEQIVAAIGKAEQQTSGEIRVYVEHRCR
                     YVNALDRAAEVFAGLKMEKTAARNAVLVYIALKSRQLAIYGDKGIHEKVGEAFWNEQV
                     GHIISHFNRSDYAGGIARVVTEIGEALKAHFPYDKGTDTNELPDDIVFGR"
     gene            complement(3136..3732)
                     /locus_tag="VKU83_08510"
     CDS             complement(3136..3732)
                     /locus_tag="VKU83_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLI93636.1"
                     /translation="MAGTPRNKSWLVIAGILALVILYIAVTYNSLVKRDENVKLMWGN
                     LENAYQRRLDLIPNLVTVVKGSSGYERQTLQQLAEARGKAQQAIVSGTGLEADNYRRL
                     EQAQATMVLSINKVIAIVEKYPDLQSTKAYLYFQVQLEGTERRIKVSRNDFNGAVAAY
                     NKMVRRFPGNIPAALFGFRPREGFESQPGAEGSPEVKF"
BASE COUNT          874 a         1085 c         1076 g          831 t
ORIGIN      
        1 acggatcatt ggaaattgga tctgcctaat gcgccttacc tgttcttcat gggcgtgggg
       61 gactatgcca ttatcaagga tagttataaa ggcaaagagg ttgcctatta tgtagagaaa
      121 gcttacgccc ccgtggcgcg caggatattt gggcatacgc cggaaatgat ggcgttctac
      181 tcgcggatca cgggggtgga tttcgcctgg ccgaagtacg atcaaatcgt cggacgtgac
      241 tatgtgagcg gggcgatgga gaacacgacc tgtacgctgc acggcgagca ggcacagcag
      301 gacgcacgcg aactaaccga cgggaaccaa tgggaagacg tgatcgcgca cgaattgttc
      361 catcattggt ttggcgatct ggtgacctgt gagagctgga gcaatctcac gttaaacgag
      421 tcctttgccg acttcagcga agagctgtgg aacgaataca aatatggcaa ggacgccggg
      481 gatgcggtga actatgccgg cattcaaagc taccttcagc aaccggagaa tgcggataag
      541 gacctggtgc ggttccatta cgccgacaag gaagatcttt ttgatcaggt cacctatccg
      601 aaagggggca ggatactcaa tatgctgcgg aattatgtcg gcgacagcgc tttctggaag
      661 tccctgaacc tgtatctcaa cacctacaag ttccagtctg cggaggcgca ggagcttcgg
      721 ctggcttttg agcaggtgac cggcgaagac cttaactggt actggaacca atggtattac
      781 gggagcggtc atccgaagct gacgatcgat tatgcctacg acgatgccgg gggcaaggct
      841 accgtcatcg tgaaccaaac ccaggacggg ggaaagatct tcaagctgcc ggtagccatc
      901 gatgtctata acggagcgtc aaaggtccgt cataaggttt gggttcagga taagagcgat
      961 accttcacct tcgcctacca aaacaggccg gacctgatca attttgacgg cgacaagatc
     1021 ctgctttgtg aaaagacgga caataaaaca ctcgataact acatccatca gtaccaatac
     1081 gcgggtctct atcttgaccg ccgggaagcc attgattttt gttcccggca cgaggacgat
     1141 cccaaagccg tggagctgtt aaaggccgcg ctcaaagatc ggttttacaa gctgcgggat
     1201 tttaccctgt caaagctgaa cttccagcag gacggcgtaa aagcggcgtt cgagccgctg
     1261 gttgcccgga tggctgagaa cgatcccagc cgggtggtca aagcgacggc gatcgaaatt
     1321 ctgggaagtc tcaagaaccc cgcgtacgaa gcgttgttca ccaaagccac caatgactcg
     1381 tcttacaccg tgtccggaaa tgcgctggag tccctgctcg ctatcgacag tgtcgctgcc
     1441 ttgaagatcg caaaggaatt tcagcgggag ccggcgaagg gcgagctcgc cgccgcgatc
     1501 gccatagtgc tcattaagaa tggtgatgag agtgattttg acgcgattac gaatacattt
     1561 gccaggatgc cggtgaccca ggcgaagttt gagttcctgc ggccctacct ttcgttactg
     1621 gccaacgtga agagccttga ccacctgaag cgcggcgtgg acgagctgct caaattcaag
     1681 gtggcgatac cggcggccta ccggaatcag acagacccct ttttcgatgc cgcgttgaat
     1741 agcctggcta caaagaaagc cgcggcggga atgcaggatc aggcggatta tatcaagagc
     1801 gcgatggaga aacagaaata ggacatggcg gggcgcaccg gttaccagcc gccgcttgca
     1861 ccaccgcctc cgccgctgcc gccgccgaat ccgccgccaa agccgccacc gccgccgccg
     1921 ccccagccac ctccgccgcc ccagcggtcg ccaccgccgc caaagccgcg gccgaccatg
     1981 gtgccgagaa agaatggcca ccagccaccg ccgccgaagt agccgccacc gcccctaccg
     2041 ccgccgcggc ggatgatgat caggaagatg ataataagga tgatcacgag gatgccgatc
     2101 ccattgccgc ctccacgccg atgaccaccg ccttcggcat ggtactgccc ggctgccgct
     2161 ttgatcagcg cgtcgactcc ctggtcgaag ccccggtaat agttatcgtt gccgccgccc
     2221 ctgaaattgg gaacgatctc attatcgatg atcgacttgg cggtaatgtc agggatcgcg
     2281 ccttccaaac cgtacccaac cgcaatgttg atctgccggt ctttgatgtc ggcaacaatg
     2341 acgacgccgt tgttgttttt cttactcccg actccccagg tacgcaggat ctttagcccg
     2401 tagtcgtcga tggcagcgcc gccgatatcc ggtaccgtta caataacgat ctgggtgccg
     2461 gtactgtcgt cgtaggcttc cagcttccgg tccagcgcat cgatctgatc cggcgtcatc
     2521 acatgggcgt agtcattaac aggggtcgcg ggattgggct ttggcgggat ttcctgtgct
     2581 tgggccttca tggaagcgac ggcaaggcag gaactgaata aaaatatcgt gaagagcttg
     2641 tacatgtctt tttagttatt accggccgaa aacaatatcg tcggggagtt cattggtgtc
     2701 cgtgccctta tcataaggga aatgggcctt cagggcctcg ccgatctccg tgaccacgcg
     2761 ggcgatgccg ccggcatagt cggatcggtt gaaatggctg atgatatgac cgacctgctc
     2821 attccagaag gcctcgccta ctttctcatg gatccccttg tcgccgtaaa tagccagttg
     2881 acggctcttc agggcgatat acacgaggac cgcattccgg gcggcagtct tctccatctt
     2941 caggccggcg aaaacctcgg cggcccgatc gagtgcattc acataccggc agcgatgctc
     3001 gacatatacg cggatctcgc cactggtttg ctgctcggct ttgccgatag cagcaacgat
     3061 ttgttcctgt tcggccgccg taaacagttt cttttttgaa aaaaatggga tatgcaaacg
     3121 ataaattttt aaggtttaga atttgacctc gggcgatcct tcggctcccg gctggctttc
     3181 aaagccctcc cggggcctga acccaaacag tgctgccgga atattgcccg ggaaccgccg
     3241 caccattttg ttataggctg caacggcgcc gttaaaatcg tttctcgaca ccttaatcct
     3301 gcgctccgta ccttccagct gcacctgaaa atagagataa gccttggtcg actggaggtc
     3361 ggggtatttc tcaacgatag cgatcacttt attgatcgac aggaccatgg tcgcctgcgc
     3421 ctgttccagc cgccggtaat tgtccgcttc caggcccgtt ccgctgacga tagcctgttg
     3481 cgccttcccc cgggcctccg ccaactgctg cagcgtctgg cgctcataac cggaggagcc
     3541 ttttaccacg gtgaccagat tggggataag atccaggcgc cgctggtagg cgttctcaag
     3601 attgccccac atcagcttca cattttcgtc ccgtttgacc aggctgttat aggtcaccgc
     3661 tatatatagg atgacaaggg cgagaatgcc tgcaatcacc agccagctct tgttgcgtgg
     3721 agtaccggcc atggggatca gaacttaact tgaggggctt tctcggccgc ggcatccgcg
     3781 gtaaatccgg tctttggccg gaacccaaac atccccgaca acaggttcat cgggaatgat
     3841 ctcacccgca cgttatactc attgac
//
LOCUS       DATGKE010000493         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1497702, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000493 DATGKE010000000
VERSION     DATGKE010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497702"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VKU83_08515"
     CDS             complement(<1..945)
                     /locus_tag="VKU83_08515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLI93637.1"
                     /translation="MSVRGFKFLLVAAFSIVSIFFFNVSYGQQNPPAGEGAGQPDTAK
                     INPSGLILEHIANGHEFHFFTFHHRIVSIPLPVILYSAQKGWSFFMASRFEHGEKTYA
                     GYRMLTEDYISANRLDEKVYKAGQIVAVRADGSIDPAVRVFDFSLGRNVTQMLISILL
                     LVWIMTSAAKKYKQGIGVRSAPSGMQSLLEPIIIFIRDEVAKVNLGNKYEKYMPFLLT
                     IFFFILINALLGLIPGTANVVGNIAFTGALGVLAFIAIMFSTGKHYWLHIVNPPVPLG
                     VKPIMIPVEILGIFTKPFALIIRLFANMISGHIIILSFI"
     gene            complement(1183..>2383)
                     /locus_tag="VKU83_08520"
     CDS             complement(1183..>2383)
                     /locus_tag="VKU83_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93638.1"
                     /translation="NAVAALNAFVQEHITGVQIVQAFAAEKREARKFDKINREHRNAN
                     INAIFAYSVFFPVVEIILAVSTGLLVWWGATQIMGMPESQARELGGKIISFFLYLNLL
                     FRPLRVMADKFNVLQMGMVASERVFRVLDNNDVAADQGTYNPGLVKGKVEFDHVNFAY
                     TENRYVLKNISFTMQPGQTVAIVGHTGSGKTSIISLLNRLYHIQEGEIRIDDVPIEQY
                     QLAALRRCIGVVLQDVFLFSGSVLDNITLRNEDIPIEKVEEAARLIGMHDFIMRLPGG
                     YQYNVMERGSSLSLGQRQLLSFVRALLYNPSILILDEATSSVDTESEQLIQAAIEKLI
                     AGRSSIVIAHRLSTIRKADQIIVLDKGEIREIGTHDQLLAKGGFYSKLHEMQFRKPPA
                     GVERKAS"
BASE COUNT          545 a          658 c          664 g          516 t
ORIGIN      
        1 gataaagctc aggatgatga tatggcccga gatcatgttg gcgaaaagcc ggatgatgag
       61 cgcgaaaggc ttggtgaaaa tgccgaggat ctcgaccggg atcatgatcg gcttaacccc
      121 gagtggcacg ggcgggttaa cgatatgcag ccaatagtgc ttgccggtgc tgaacatgat
      181 cgcgataaag gccagcaccc cgagggctcc cgtgaaggcg atattgccga cgacgttggc
      241 cgttccgggt atcagcccca acagtgcatt gatcaggata aaaaagaaaa tagtcagcag
      301 gaagggcatg tatttctcat acttgttgcc gaggttgacc ttggccacct cgtcccggat
      361 gaaaatgatg atcggctcga gaaggctttg cattccgctt ggggcggatc tgacgccgat
      421 accctgcttg tatttttttg ccgcgctggt catgatccac accagcagca ggatcgagat
      481 cagcatttgc gtgacattcc ggccgagcga aaaatcaaat accctgacgg ctggatcgat
      541 cgacccgtcg gccctgaccg cgacgatctg gccggcttta tatacctttt catccaaccg
      601 gttggccgaa atatagtcct ccgtcagcat ccggtatccc gcataagtct tttcgccatg
      661 ctcgaagcgc gaagccatga aaaaagacca gcctttttga gcgctataca ggatgaccgg
      721 caaagggatg gaaacgatgc ggtggtgaaa ggtgaaaaaa tgaaattcgt ggccgttggc
      781 aatatgctcg aggatcagtc cggacggatt aattttggcc gtgtcgggct gcccggcgcc
      841 ttcccctgcg ggggggtttt gttggccata cgacacatta aaaaaaaaga tactaactat
      901 gctgaaagcc gctaccaata agaatttgaa ccctctgacg gacatatatc cttccaaatt
      961 tgctgcaaag ataggggcgt ggtggcagat tatgaaaagc tggcgaaaaa atccgggggg
     1021 ccggcccgtc gcggggacgg tcccatgcct tgaccttaat tgggccgaat tcggcccaat
     1081 taggtgggaa gatagggggg tacctcctgc ttgccgccca ggacgcccag ctaccccgtc
     1141 taggacgccg ccgccacccc ctgggggcgc tccgccaccc gctcaggacg cctttcgctc
     1201 gacccctgcc ggaggcttgc ggaactgcat ttcatgcaac ttggagtaaa acccgccttt
     1261 tgccaacagc tggtcatgcg tgccgatctc ccggatctcg cccttgtcga gtacgatgat
     1321 ctgatctgcc ttgcggatcg tggacaggcg gtgggcgatc acgatggaac tccggccggc
     1381 tatgagcttt tcgattgccg cctggatcag ctgttcggat tctgtatcca ccgaagaggt
     1441 cgcctcatcc aaaatgagaa tcgatggatt gtacaacagc gcccgcacaa aagacaaaag
     1501 ctgccgctga ccgagggaaa ggctgctccc cctttccatg acgttgtatt gatagccccc
     1561 gggcaaccgc atgatgaaat cgtgcatgcc gataagcctg gcggcctcct ccactttttc
     1621 gatcgggatg tcctcgttcc ggagcgtgat attgtcgagg accgagcctg aaaaaaggaa
     1681 tacgtcctgc aggacgacgc cgatgcacct tcgcagcgcc gccagctgat attgctcgat
     1741 cggcacatcg tcgatccgga tctcaccttc ctggatatga taaagccggt tcagcaaact
     1801 gatgatcgac gttttcccgc tacccgtgtg gccgacgatg gcgaccgttt ggccgggctg
     1861 catcgtaaag gaaatattct tcagcacgta ccggttctcc gtgtaagcaa aattgacatg
     1921 gtcgaactct accttccctt ttacgaggcc gggattatac gtaccctggt ccgcggcgac
     1981 atcattgtta tccaggaccc tgaatacccg ctcgctggcc accatgccca tttgaaggac
     2041 gttgaactta tcggccatga cccggagggg gcggaacagc agattgaggt aaaggaaaaa
     2101 tgaaatgatc tttcccccga gctcgcgggc ctggctttcg ggcatcccca tgatctgggt
     2161 cgccccccac caaaccagca ggcctgtact gacggcgagg atgatctcaa cgaccgggaa
     2221 aaagaccgaa taagcgaaga tcgcattgat attcgcgttc cggtgttcgc ggttgatctt
     2281 gtcgaatttg cgggcctcgc gcttctccgc cgcaaacgcc tggacgatct ggacgcctgt
     2341 tatgtgttcc tgaacaaaag cgttgagcgc ggccaccgcg ttc
//
LOCUS       DATGKE010000494         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_548403, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000494 DATGKE010000000
VERSION     DATGKE010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548403"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VKU83_08525"
     CDS             complement(<1..327)
                     /locus_tag="VKU83_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLI93639.1"
                     /translation="MNTTQDTLFATTAIYTWEQSIKRATGFFDKCSDDDMLQPIVTGK
                     NRVIYLLGHLIAVHDRMFPLLDLGERSYPHFDELFLINPDNSDAILPAVAQLRDAWTS
                     VNERLTS"
     gene            complement(366..1184)
                     /locus_tag="VKU83_08530"
     CDS             complement(366..1184)
                     /locus_tag="VKU83_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLI93640.1"
                     /translation="MQKLLYLLAIGLVLNGCVPKRKLHAAQADARALRADSARQAARI
                     ASQGANIDQLNSQIDDLNKRLTDLQNRSGLTEDQLNQSKEQIAEQRRRLAQLQALMDQ
                     QKNAIQDIRKKMSDALVGFNSKDLSVSIKNGKVYVSLSENLLFPSGSAVVNPKGKEAL
                     GKLATVLNTNPDITVDIEGHTDSVPIHGRYPDNWALSVARSTAIVRILTTDYLVDPVR
                     VIASGHSSYDPVQPNGTPEGRALNRRTEIILSPKLDELYKLLESSPGESGGEPK"
     gene            complement(1238..2110)
                     /locus_tag="VKU83_08535"
     CDS             complement(1238..2110)
                     /locus_tag="VKU83_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93641.1"
                     /translation="MQLRYSLYGIAALSLILSTACRSSRGSSGETPAATWQQQPLTID
                     GSDNDWTKPLPGYSNSERVSYAVSNDNRDVYILISAKDPQEQQKIIQGGMTVWVNAKA
                     DKTQAGAIGIGYPLDTRMDHDRTLMEEAQPDRYKATNGATLQDKKNYTLYGFNRDSAA
                     GTYTYGDDNPQGIRMRMDYNNSGELIYEAAFPLDLLYPGSDPAASYAAKSIAVGIFVD
                     GLPPDARIPRGNGGGPGVGVGVGGGMGFGSFGSGGGVGVSLGIPIGGGRTKQLFRDTE
                     LWQVVQLTSVKRGF"
     gene            complement(2242..>2569)
                     /locus_tag="VKU83_08540"
     CDS             complement(2242..>2569)
                     /locus_tag="VKU83_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLI93642.1"
                     /translation="HFKPDITGFSLVKVPMEAGDLLIFNSSQPHGIRPNRSDDKVRIA
                     QYISMMPAEEDNESLRQWRISSWRDRIAPEGYAFPGDPRGWERTKYSTAELSPLGRKL
                     LGLDRW"
BASE COUNT          493 a          707 c          781 g          588 t
ORIGIN      
        1 cgaggtgagt ctttcattga cgctggtcca ggcatcgcgc aactgggcga cggcgggcag
       61 gatggcgtcg ctattgtcgg ggttgatcag gaacagctcg tcgaaatgcg gataggacct
      121 ctcccccagg tccagcagcg ggaacatccg gtcatggacg gcgatgagat ggccaagcag
      181 atagatgacg cggttcttgc ccgtgacgat aggctgtaac atatcgtcgt cgctgcattt
      241 atcgaaaaag ccggtggccc ttttgatgga ttgttcccac gtgtagatcg ctgtcgtggc
      301 gaacagggta tcctgagttg tattcatggg tacgaaggta gattttgctc cgcacgcggg
      361 gaaaattatt tgggctcgcc gccggactct ccggggctcg actccagcag cttgtacaac
      421 tcgtccagtt tgggggaaag gatgatctcg gtgcgccggt tgagggcgcg gccctcgggt
      481 gtgccgttgg gctgtacagg atcataggag ctgtggcccg aggcgatcac ccggaccggg
      541 tctaccaggt agtccgtggt gaggatgcgc acgatagccg tggaacgggc cacgctgagc
      601 gcccagttgt cggggtaacg gccatggatg ggcaccgaat cagtatggcc ttcgatatcc
      661 accgtgatat ccgggttggt gttcagcacg gtggccagct tccccaatgc ctctttaccc
      721 ttgggattga cgacggcgct gccggaggga aaaagcaggt tttccgaaag gctcacatag
      781 acctttccat tcttgatgga gacgctgagg tccttggaat tgaagccgac aagcgcatcg
      841 ctcattttct tgcggatatc ctgaatggca tttttctgct ggtccatcaa tgcctgcagc
      901 tgggcgagac ggcgccgttg ttcggcgatc tgttccttgc tctgattgag ctggtcctcg
      961 gtgagaccgg acctgttctg cagatcggtc agccttttat taaggtcgtc gatctggctg
     1021 ttcagctgat cgatgtttgc cccctggctg gcgatccgag cggcctgccg ggcactatcc
     1081 gcgcgaaggg cgcgggcgtc cgcctgagcg gcgtgtaatt tacgtttggg aacacaacca
     1141 tttaatacta agccgattgc caggaggtag aggagttttt gcatggtagt agcattagtt
     1201 gatgctaagc taccatcaat ctttgggcca cgccctatca aaaacctctt tttacgctgg
     1261 tgagctggac aacctgccag agctcggtgt ccctgaacag ctgtttggtc cggccgccgc
     1321 cgatgggtat gcccagtgag acgcctacgc cgccaccgct gccgaatgat ccgaagccca
     1381 tcccaccgcc tacgcctact ccaacgcccg gtccgccgcc gttacccctg ggaatacgcg
     1441 catcgggcgg aaggccgtcg acaaaaatac ccaccgcaat gctttttgcg gcataggaag
     1501 cggccggatc cgagccggga tacagcaggt ccaaagggaa ggccgcctcg tagatgagct
     1561 ccccggaatt gttgtagtcc atgcgcatcc ggatgccctg cggattgtcg tcgccgtaag
     1621 tataggtgcc ggcggccgaa tccctattaa aaccatagag ggtatagttc ttcttatctt
     1681 gcagggtagc gccattggtc gccttatagc ggtcgggctg cgcttcttcc atcagggtac
     1741 ggtcgtgatc catccgggtg tccagcggat agccgatacc gatggcgccg gcctgtgtct
     1801 tgtctgcctt ggcattgacc cagacggtca tgccgccctg gatgatcttt tgttgttcct
     1861 gcggatcttt cgccgagatc aggatataga cgtcccggtt atcgttgcta accgcatagc
     1921 tgaccctttc cgaattggaa tagccgggaa gcggtttggt ccagtcgtta tccgatccgt
     1981 cgatggtcag cggctgctgt tgccaggtgg cggccggcgt ctcgccgctg ctcccccgtg
     2041 aagagcggca ggctgtcgaa aggatcaacg agagggctgc tattccgtat agcgaatacc
     2101 gtaactgcat cctgcaaaga tagcccgcat tttgtctatg agcggcaaat acgccctgtt
     2161 aaaaattagc gcatttgccg caatgggctt atgattgacc tatcggtcgg gcggccaacg
     2221 ccgccggaag cgtcaagccc attaccagcg gtcgagaccc aacagcttgc ggcccagtgg
     2281 gctaagctcc gctgtggaat attttgtccg ctcccaaccc cggggatcgc cggggaaggc
     2341 atagccttca ggcgcgatcc tgtcccgcca ggacgagatg cgccattgcc gtagcgactc
     2401 gttatcttct tcggccggca tcatggagat gtattgagcg atgcggacct tgtcgtcgct
     2461 ccggttaggc cggatgccat gcggttgaga gctgttgaaa ataagaagat cgcccgcctc
     2521 catcggcact ttgaccaggg agaacccggt gatgtccggt ttgaaatga
//
LOCUS       DATGKE010000495         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_529685, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000495 DATGKE010000000
VERSION     DATGKE010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529685"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VKU83_08545"
     CDS             <1..404
                     /locus_tag="VKU83_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLI93643.1"
                     /translation="YAPLLEPAGFELKKAYPGKAWIGDPRMEEREGLIITGNTLRGTP
                     LYTAGLDVDDKITSIDNQDIRSYADLNAVLSRLHPGTAVSIRYFHRNEEQDAQITPVE
                     NPNLMIGTFESAGLTVTPAVTQFRQQWLGTK"
     gene            complement(428..1534)
                     /locus_tag="VKU83_08550"
     CDS             complement(428..1534)
                     /locus_tag="VKU83_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93644.1"
                     /translation="MEHNERQIPTSRFLFSHKVLRALLITGICSFVIITACNNGRSKA
                     GRYGDPGAKAGETKDTAAPAPIAAPLDTALYNKLLVHLANGDSSHRWPVRTAYPDAGA
                     ILPFHRIVAYYGNLYSKNMGILGEYPPNEMLRRLKVELRKWQEADPVIPVMPALHYIA
                     VTAQGAPGKDGKYRARMPFKQIDSVLHIAARINAIVFLDIQVGFSNVQSEVPLLEKYL
                     KMPNVHLGVDPEFAMNKSGRKPGTVIGTLDASDVNWCVGYLTRLVKENNLPPKILIVH
                     RFTKNMLTDARKIHPTPEVQFVLDMDGWGPVSLKTDSYKSYVADFPVEFTGFKLFYKN
                     DTRRPRGVNLKETQMMTPAQVLAQKPMPIYIQYQ"
     gene            complement(1639..3267)
                     /gene="pgi"
                     /locus_tag="VKU83_08555"
     CDS             complement(1639..3267)
                     /gene="pgi"
                     /locus_tag="VKU83_08555"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLI93645.1"
                     /translation="MFPQQKPTETAAWNQLQQHYQAEIKGTHMRSLFSADPDRFSRYS
                     LRLDDILFDFSKNIVTGKTLELLRQLAAECKLKEAIEAMFSGEKINGTEGRSVLHTAL
                     RNFSGNPVYSDGKDVMPGVRQVLAQMKDFCRRVHSGEWKGYNGKKIRYIVNIGIGGSD
                     LGPVMVTEALRPYWKPGIEAHFISNVDGTQIVETLKRVTPDETLFLVASKTFTTQETM
                     TNAHTARDWFLQTAKDEAHVARHFVALSTNEKEVVKFGIDKANMFEFWDWVGGRYSLW
                     SAIGLSIALTIGYEHFETLLKGAHQTDLHFRNTAFEKNIPVTMALIGLWYTNFFGSQT
                     EAILPYDQYMHRFAAYFQQGNMESNGKSVDRSGDPVAWSTGPVVWGEPGTNGQHAFYQ
                     LIHQGTVMIPCDFIAPAITHNPVGDHHVKLLANFFAQTQALMNGKTKQELEAEKVPAA
                     LVPYKGFSGNRPTNSFLIKEITPFTLGELIALYEHKIFVQGVIWDIFTFDQWGVELGK
                     QLAGKILPELQNNEEIKSHDSSTNGLINAFKKMR"
     gene            complement(3290..>3903)
                     /locus_tag="VKU83_08560"
     CDS             complement(3290..>3903)
                     /locus_tag="VKU83_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HLI93646.1"
                     /translation="ENTANRGGGSCYELEEIRSIRELCTERQLKLHLDGARLFNALVA
                     KNQTARQYGSLFDSISVCLNKGLGCPIGSILMGTAAFIRQARRVRKVFGGGMRQAGFM
                     AAAGIYALEHHVDRLAIDHTHAKQIAAALEKKSFVRNILPVETNIIIFELEAAVPAAT
                     FTQRLRQSDILAIPIAAQQVRFVTHLDISPAMVEQTVVAIEQL"
BASE COUNT          840 a         1075 c         1049 g          939 t
ORIGIN      
        1 actatgcccc tttacttgaa cctgcggggt tcgagctgaa aaaagcttac ccgggcaagg
       61 cctggatcgg agaccccaga atggaggagc gggaaggatt gatcataacc ggaaatacgt
      121 tgcgcggtac gcccctttat actgcaggcc ttgacgtgga cgataagatc acgtcgatcg
      181 ataaccagga tattcgatcc tatgcggacc tgaacgccgt cctcagccgg cttcatcccg
      241 ggactgccgt ttcaatccgg tacttccatc gcaatgaaga acaagacgca caaattaccc
      301 cggtggagaa tccgaacctc atgatcggga ccttcgaatc cgcgggcctg acagtaaccc
      361 cggcggtgac gcaattccgt cagcagtggc tgggaaccaa gtagttggct gcttccgtcc
      421 ggcgttgtta ctgatactga atatagatcg gcatgggctt ttgtgcaagc acctgggccg
      481 gcgtcatcat ctgcgtctcc ttgaggttga cgccgcgggg cctgcgggta tcgttcttat
      541 agaataactt gaagcctgta aattccaccg ggaagtcagc gacatagctt ttatagctat
      601 ccgttttcag gctgaccgga ccccacccgt ccatgtccag gacaaactgc acttccgggg
      661 tcggatggat cttcctcgcg tcggtaagca tgttcttggt gaagcggtgc acgatcagga
      721 tcttaggcgg aagattgttc tcctttacaa gcctggtcag gtaacccacg caccaattga
      781 cgtccgatgc gtccagggtg ccgatcaccg tacccggttt tctgcccgat ttattcatgg
      841 caaattccgg atctacgccg aggtggacgt ttggcatctt aagatatttt tccagcagcg
      901 gcacttccga ttgaacgttg ctgaatccga cctggatgtc caggaagacg atggcattga
      961 tgcgtgcggc gatgtgcagg acggaatcga tctgtttgaa cggcattctc gcgcgatatt
     1021 tgccatcctt cccaggcgcc ccttgcgctg tgacagcaat ataatgcaag gcaggcatca
     1081 cgggaatgac cggatcggct tcctgccatt tcctcagctc caccttcagg cggcggagca
     1141 tttcgttcgg tggatattcg ccgaggattc ccatattctt ggaatacaag ttaccgtaat
     1201 aggccacgat ccggtgaaaa ggcaggatcg cccccgcgtc aggatacgcc gtacggaccg
     1261 gccagcgatg agaagaatca ccgtttgcca ggtgcaccaa taatttattg tagagcgcgg
     1321 tgtccagcgg cgcggcgatc ggtgctggcg ccgcagtatc tttcgtttcc ccagccttcg
     1381 cgccggggtc gccgtacctg ccggctttcg agcggccgtt gttgcaagcg gtgatgatga
     1441 cgaaactgca aataccggta atcagaagtg cccggaggac tttgtgggaa aaaagaaatc
     1501 gggatgtggg aatctgacgc tcattatgct ccatggtgta ggctgccgta gattttttgt
     1561 gatgcgggca aagttaacgg aaaacgatcc caatcatttt ttattttcta ataaaaactt
     1621 aaaaaggacc tgcctttttt accgcatttt cttgaacgcg ttgatgagcc cgttcgtaga
     1681 agagtcatga gacttgatct cctcgttatt ctgcagctcc ggcaggattt tccccgcgag
     1741 ctgcttgcct agctctaccc cccattgatc gaaggtaaag atgtcccaga tgaccccctg
     1801 cacgaagatc ttgtgttcat acaaggcaat gagctcccca agggtgaatg gtgttatttc
     1861 tttgatcagg aacgagttag taggccggtt gccgctgaag cctttgtagg gaaccagcgc
     1921 cgcgggcact ttttccgctt ccagctcctg cttcgtcttg ccattcatca gcgcctgtgt
     1981 ttgagcgaag aaattggcga gcaacttgac atggtggtcg ccgaccggat tatgggtgat
     2041 tgcgggcgca atgaaatcac agggaatcat caccgtcccc tggtggatca actggtaaaa
     2101 agcatgctgg ccattggtgc ccggttcgcc ccatacgacg gggcccgtcg accaggcgac
     2161 tggatcgccg ctacggtcga cactcttacc gttgctttcc atattgccct gctggaagta
     2221 ggcggcgaaa cgatgcatat actggtcgta gggaaggatg gcttctgtct gcgatccgaa
     2281 aaagttggtg taccacaagc cgatcagggc catcgttacg ggaatattct tttcaaaggc
     2341 agtgttccga aaatgcaggt cggtctggtg tgcgcctttg aggagcgtct cgaaatgttc
     2401 gtagccgatc gtcagggcga tcgatagccc gatcgcgctc caaagggaat accggccgcc
     2461 tacccagtcc cagaactcga acatattcgc cttgtcgatc ccgaacttaa ccacctcctt
     2521 ttcattcgtc gacagagcca cgaagtggcg ggcgacatga gcttcatcct ttgcggtctg
     2581 gaggaaccag tcacgtgctg tatgcgcgtt ggtcatcgtc tcctgggtcg taaacgtttt
     2641 cgaggcaacc aggaaaaggg tttcgtcagg ggtaacccgt ttcagcgttt cgacgatctg
     2701 ggtgccgtcc acattagaga taaaatgcgc ctcgatgccc ggtttccagt atggccggag
     2761 ggcctctgtc accataaccg gacccaggtc gctgcccccg atgccaatat tgacgatata
     2821 cctgatcttt ttcccattat atcccttcca ttcgccgcta tggacacgcc ggcaaaaatc
     2881 cttcatctga gcaagcacct gccgcacgcc gggcatgaca tccttgccgt cactataaac
     2941 aggattcccg gaaaaattgc gcagcgcggt gtgcagcacc gaccggccct cggtgccgtt
     3001 aatcttttca ccgctgaaca tggcttctat cgcctctttc agcttacatt ccgccgccag
     3061 ttgccgcagc agctccagtg tcttcccggt tacgatattt ttggaaaagt cgaaaagtat
     3121 atcatccagc cttaacgagt accgggagaa cctatcagga tcggcgctga acaatgagcg
     3181 catgtgcgtt ccctttattt ccgcctggta gtgctgctgc aattggttcc aggctgccgt
     3241 ttctgttggt ttctgctgcg gaaacataaa aaagtttttt taaaggtaac tacaattgct
     3301 caatggcaac caccgtctgc tccaccatgg cgggcgagat atcgaggtgc gtgacgaatc
     3361 gcacctgttg cgcagcgatc ggaatggcga ggatgtcgga ttgccttaac cgttgtgtga
     3421 acgttgccgc cggcacggcc gcctccaatt caaagatgat aatattggtt tccaccggga
     3481 gaatgttccg gacaaagctt ttcttctcca gtgccgccgc aatttgtttt gcatgcgtat
     3541 gatcgatggc gagccggtcg acatgatgct ccagcgcata gatgcccgcg gcggccatga
     3601 atccggcctg ccgcatgccg ccgccgaaga ccttcctgac acgccttgcc tggcggataa
     3661 acgccgccgt ccccatcagg atgctgccga tcgggcaacc gaggccctta ttcaggcaga
     3721 ccgaaatgct gtcaaataag ctgccgtatt gccttgcggt ctgattctta gccacaagtg
     3781 cgttgaagag cctcgctccg tcaagatgca gcttcagctg gcgctcggtg caaagctccc
     3841 ggatgctgcg gatctcctcc agttcataac agcttccccc gccgcggttg gcggtgttct
     3901 cca
//
LOCUS       DATGKE010000496         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_462433, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000496 DATGKE010000000
VERSION     DATGKE010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462433"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1387
                     /locus_tag="VKU83_08565"
     CDS             251..1387
                     /locus_tag="VKU83_08565"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HLI93647.1"
                     /translation="MRLFILSILSFISFSAFSQQYYLFVGTYTEGSPASRGSKGIYVY
                     RFNAATGDATPVSTTMADNPSYLAIASGGHFVYSVNETHGAIPGGVSAFSFDKATGKL
                     KLIDKQASGGADPCYVSVDAGRKWALVANYSGGSLSALPIAADGSLRPLTELIQHMGA
                     GPNKARQEQAHVHSTILSPDEKYLVVADLGMDQLRVEHFNPAATKWPLTPAADSIVKI
                     QPGYGPRHTAFLPGRPYVYLINELSGMVDAFRYSDGKFTLIQAISSHPAGYHGEIGSA
                     DIHASGDGRFLYASNRGDANNIAVFAINGATGKLTWKGVVPTGGKTPRNFLIDPTGKW
                     LLAANQDGSNVVIFRLDPVTGMPSPAGKQLSIPSPVCLKMTPVN"
     gene            complement(1444..2601)
                     /locus_tag="VKU83_08570"
     CDS             complement(1444..2601)
                     /locus_tag="VKU83_08570"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HLI93648.1"
                     /translation="MFIIDAHLDLAMNAMEWNRDLRSPVSAIRRREQGLADKPDRGRG
                     TVSLPALREGGIGLVVATQIARWVAPDNPLPGWHSPWQAWAQTQGQLAWYQAMEEAGE
                     MVMVKDLPGLERQVKLWTQEERRTDEPGGPEGNSGGRTDGGGGPVDAEEGQNGKRPVG
                     YILSLEGADSLVATEHLHRAYEYGLRAVGPAHYGPGRYANGTDSSGRLSPAGKGLLRE
                     MQRLNIILDATHLCDDAFWDAMEVYDGPIWASHNNCRALVDHNRQFSDEMIRALVARG
                     AVIGGALDAWMMVPGWVRGKSDPREMKCNLEKLADHMDHICQIAGNASHIGIGTDLDG
                     AFGKEQCPYDLETIADLHALRPILAARGYSAHDISGVFHGNWLRFLRKAWG"
BASE COUNT          539 a          939 c          730 g          611 t
ORIGIN      
        1 gaacggccgg cagcggggga atagcagatt tgccgctgtc agcatgggcc atctgcattg
       61 tgtcatgccc ggctgtcgcg gtagtgtcgc ccggtgcggc agccttgtgg tccgcattgt
      121 tgcaggcaac cattccgatc agcagggcag cgggaagaaa gtggatcttg cgcatgttaa
      181 ggttaatttt aagtaataaa aatagtactt ttaccattac ctgcggattc attcatccaa
      241 tcgctttttt atgcgcctct tcattctttc cattctgtcg ttcatttctt tctccgcctt
      301 tagtcagcag tactacctat tcgtcggcac ctataccgaa ggatcgcctg ccagccgtgg
      361 cagtaagggc atatatgtgt accggttcaa cgccgccacc ggcgatgcga cgcccgtcag
      421 cacgacgatg gctgacaatc cctcctacct cgctatcgcc agcgggggcc acttcgttta
      481 tagtgtgaat gaaacgcatg gggccatacc gggaggcgta agcgcttttt ccttcgacaa
      541 agcgaccggc aaactgaagc tcatcgacaa gcaagccagc ggcggagccg atccctgtta
      601 tgtttcggta gacgccggcc gaaaatgggc gctcgtcgcc aattacagcg gtggcagcct
      661 gtctgcgctg cccatcgccg cggacgggtc gcttcgtccc ctgacggagc tcatccagca
      721 catgggcgcc gggccgaata aagcgcgtca ggagcaagct cacgtgcatt ccacgatcct
      781 ctctccagat gagaaatatc tggtagtcgc tgacctcggg atggaccagc tgcgggtcga
      841 acatttcaat cctgctgcca caaaatggcc cctcacacct gctgccgact cgatcgtcaa
      901 gatccaaccc ggatatggcc cgcgtcacac tgccttcctg cctggcaggc catacgttta
      961 cctgatcaac gaactcagcg gcatggtcga cgcgtttcgc tattccgacg gaaaatttac
     1021 actgatacaa gcgatttctt cgcaccccgc agggtatcat ggcgagatcg gaagcgcaga
     1081 tatccatgcc tcgggtgatg gtcgattcct ctatgcttcc aatcggggcg acgccaataa
     1141 catcgccgtt ttcgccatca acggcgcaac aggcaaactg acatggaagg gcgtagtacc
     1201 gaccggcggc aaaacgccac gcaattttct gatcgatccg acgggcaaat ggctgctggc
     1261 cgccaaccag gacggctcca atgtggtcat cttcaggctc gatccggtga ccggaatgcc
     1321 ttcgccggcg ggcaaacaac tgtcgatccc gtccccggtc tgtctcaaaa tgaccccggt
     1381 gaattaacgg ttgcctggtc agcagctgcc ggccgcaggc gaaagcccgc tttgcacccg
     1441 gctttatccc caggcctttc gcagaaaccg cagccagttg ccatggaaca cgccgctgat
     1501 gtcgtgtgcg gaatagcccc gcgccgcgag aatcggccgc agcgcgtgca aatctgcgat
     1561 cgtctccagg tcatacgggc attgctcctt gccaaaagcg ccatccaggt cggtgccgat
     1621 cccgatatgc gatgcatttc ccgcgatctg gcaaatatga tccatgtggt cggcgagctt
     1681 ttccaggttg catttcattt cccgggggtc cgatttcccg cggacccagc cggggaccat
     1741 catccacgca tcgagggcgc cgccgatcac cgcccctctc gccaccagcg cccggatcat
     1801 ctcgtcgctg aactgccggt tatgatctac cagcgcccgg cagttgttat ggcttgccca
     1861 gatcggcccg tcatagacct ccattgcatc ccagaaggca tcgtcgcaca gatgcgtcgc
     1921 atcgaggatg atattcagcc gctgcatctc ccgcagcagg cccttccctg ccggactcag
     1981 ccggcccgag ctgtcggttc catttgcata tcgccccgga ccgtaatgtg ccgggcccac
     2041 cgccctgagt ccgtattcat aggcccggtg caggtgctct gtggcgacca gcgaatccgc
     2101 gccctccaga cttagaatgt agccaaccgg ccgctttccg ttctgccctt cctccgcatc
     2161 aaccggtccc ccgccgccgt cggtccgccc cccggaattc ccttccggtc ccccaggctc
     2221 atccgtccgc ctttcctcct gcgtccagag ctttacctgg cgctccaggc ctggcaggtc
     2281 ctttaccatc accatttctc ctgcctcctc cattgcctgg taccatgcga gctgaccctg
     2341 ggtctgcgcc cacgcctgcc agggcgaatg ccagccgggc agcggattgt caggcgccac
     2401 ccatctcgct atctgtgtgg ccaccaccag gccgatcccc ccttcccgca acgcgggcag
     2461 cgacaccgtc cctctccctc tgtccggctt gtcggccagg ccctgttcgc gccgccggat
     2521 ggcacttact ggtgaccgta agtccctatt ccattccatg gcgttcatag cgaggtcgag
     2581 atgggcatca atgatgaaca taagacgata ttttttggcg ccctcaaata tagcgttcct
     2641 tcctatattt gtttcaccca aatccagcgc acatgcaacg attcacctgc ctctcccttt
     2701 cgatgctctt atggagcctg cccgcacttt gccagctgac ggtctaccgc ctgcaaacgg
     2761 aaaacctgac cgatccgctc tccctcgaca gcaaatcacc gcgattcagc tggcagctg
//
LOCUS       DATGKE010000497         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1443422, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000497 DATGKE010000000
VERSION     DATGKE010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443422"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..665
                     /gene="atpF"
                     /locus_tag="VKU83_08575"
     CDS             171..665
                     /gene="atpF"
                     /locus_tag="VKU83_08575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLI93649.1"
                     /translation="MDLLRPDLGLFVWNVLGFLIVLFLLRAFAWKPILKSLKERESGI
                     ADSLAIAEKVKAEMNQMKSENEALLNKAREERAQLLKEARDTRDKIVGEARDQAKAEA
                     ARIIADAQSAIEQQKMAALTEVKNQVGSLVVEVAEKILRRELADKGEQENYIRQLTNE
                     SKLN"
     gene            667..1233
                     /gene="atpH"
                     /locus_tag="VKU83_08580"
     CDS             667..1233
                     /gene="atpH"
                     /locus_tag="VKU83_08580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HLI93650.1"
                     /translation="MTPNPRLAARYAKSILDLAVEKGQLEDIYKDMVYLRDLFRASQE
                     LVSVLQSPIIRTDKKKQILEVITKGRISALTAAFVSLVMAKEREASLPEIAAAFIGQY
                     KEYKGIQTVKLTTAVPIGQEVRQVVLDKVKADRKAQHVELDAAVNAALIGGFVLEIGD
                     ELIDASVAYDLNKIRQQFESNDFIYKIR"
     gene            1281..>2032
                     /locus_tag="VKU83_08585"
     CDS             1281..>2032
                     /locus_tag="VKU83_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93651.1"
                     /translation="MADIKPDEISAILRQQLSNANVSTEMEEVGTVLQVGDGIARVYG
                     LSHVSSGELVEFENGVRAIALNLEEDNVGVVLMGESGDIKEGAKVRRTGKIASINVGE
                     GMLGRVVNTLGEPIDGKGPIPGTKYEMPLERKAPGVIFREPVKEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFYKAGKPVYCIYVAIGQKASTVAGVMKT
                     LADNGALEYSVIVAASASDPAPLQFYAPFAGAA"
BASE COUNT          524 a          505 c          612 g          391 t
ORIGIN      
        1 ctggtggaag gggttgccct tcttgctgcc gtggcaggtc tgcttgcggt gttccattag
       61 cagggacaca gcctgcaacc atgcaggggc acttaaactg acatactgca tccgacgcac
      121 agggccgacg atgcagtatt ctatcaaaat cgactattaa aaataattct atggatctac
      181 tcagaccgga cttaggctta ttcgtgtgga acgtgctggg gttcctgatc gtcctctttc
      241 tcctccgggc attcgcgtgg aagcccatcc tgaaatcgct gaaggaacgg gagagcggga
      301 tcgccgattc gcttgctatt gctgaaaaag tgaaggccga gatgaaccag atgaaaagcg
      361 agaatgaggc tctcttgaac aaggcccgtg aagagcgcgc gcagcttctg aaggaagctc
      421 gtgacacccg cgacaagatc gtgggcgaag ccagggacca ggcgaaggcg gaagcggcga
      481 ggatcattgc cgacgcccaa tccgcgatcg aacagcaaaa gatggccgcg ctgacggaag
      541 tcaagaacca ggtgggcagt ctcgtcgtcg aagttgccga gaagatcctg cgccgcgagc
      601 tggctgacaa gggcgagcag gaaaattata ttcgtcaatt gaccaatgaa tcgaaactga
      661 attaagatga cgccaaaccc ccgtttagct gcgcggtacg ccaagtcgat actcgacctt
      721 gcagtggaga aaggccagct ggaggatatc tataaggaca tggtctacct gcgggacctc
      781 ttccgcgcca gccaagagct ggtgagcgtg ctgcagagcc ccattatcag gaccgataag
      841 aaaaaacaga tcctggaagt tattacgaag ggcaggatat cggcgcttac ggccgctttc
      901 gtcagcctgg tgatggccaa ggaacgggaa gcttcactgc cggagatcgc cgccgccttc
      961 atcggccaat acaaagaata caagggtatt cagacggtaa agctgaccac tgccgtgccc
     1021 atagggcagg aagtccgcca ggtggtcctc gacaaagtga aggctgaccg caaagcgcag
     1081 catgtcgagc tggatgcagc ggttaacgct gccctgatcg gcggcttcgt gctggagatc
     1141 ggcgacgaac tgatcgatgc cagcgtggcg tatgacctga acaagatccg gcaacaattc
     1201 gaaagcaatg actttattta taagatcagg taacagacca atgatcggaa cagacaacaa
     1261 acttcaaact gaatacaact atggcagaca ttaaaccgga tgaaatttca gcgatattgc
     1321 ggcagcaatt gagcaacgcc aacgtatcca ccgagatgga ggaagttggc acggtgctgc
     1381 aggtgggaga tggtatcgcc cgggtatatg gcctgagtca cgtcagcagc ggtgagctgg
     1441 ttgaattcga aaatggcgtc agggcgatag cgcttaacct ggaagaggat aatgtgggtg
     1501 tggttttgat gggtgagagc ggcgatatca aggaaggcgc aaaggtccgc cgcacgggaa
     1561 agatcgcttc catcaatgtc ggggaaggga tgcttgggcg cgtcgtcaat accctcgggg
     1621 aaccgatcga cggaaaaggg ccgatccccg gtaccaagta cgagatgccg ctcgagcgga
     1681 aagctcccgg cgtgatcttt cgtgagcctg ttaaggaacc gcttcagacg ggaatcaaag
     1741 ctatcgatgc catgatcccg atcggccgcg gccagcggga actgatcatt ggcgaccggc
     1801 aaacgggaaa aacggcgatt gcgatcgata cgatcattaa ccagaaggaa ttctacaagg
     1861 ccggtaaacc ggtttattgt atttatgtcg ccatcggcca aaaggcgtcg acggttgcgg
     1921 gtgtgatgaa gacactggca gacaacggcg cgctcgaata cagtgttatc gttgccgctt
     1981 cggcttcgga tccggcgccg ctgcaattct atgccccttt cgccggggcc gc
//
LOCUS       DATGKE010000498         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_726947, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000498 DATGKE010000000
VERSION     DATGKE010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726947"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2956
                     /locus_tag="VKU83_08590"
     CDS             <1..2956
                     /locus_tag="VKU83_08590"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLI93652.1"
                     /translation="VIRDGAYVPCSTDNGPRLRSYMVVGPGDEVAGERYPWGWEQPAF
                     NDADWPAAVPVSNPALWGFGSDNLWTLVPRTIPLMEERLQRIPRVRRWAEEANGKGGS
                     GGGRGEPEVAEARVEQMLSGKGAFVVPAHESLTILLDQTYNTVAYPELRVSGGKGALV
                     RLTYAESLVDSLGEKGDRGAIEGKVIKGNYDIFKPDGGTGRLFRPLWFRTFRYLQLEI
                     RTSDDSLRVDDLYGMYTGYPFVRKATFTSSDTSLKSIWDVGWRTARLCAGETYFDCPY
                     YEQLQYEADTRIQSLISLYVTGDDRLMRKALLDFYHSRIPEGLTQGRYPSSRFQVIPP
                     FSLWWIVMLHDYWMLRKDDAFVGQFLPAVRGVLDWFESHIDTSMGMLGPMPWWGFVDW
                     ADAFPGGDPPGANNGHSAVISLQYAYTLAQAAELFKYFGGQWAYEGQRCGRLAAELNR
                     GTYRACFDASRGEMANTPQKNSFSQHASILAILSNAIPETGRRPLMAKVLADTSLEQA
                     TFYFRFYLNQAMKAAGMGDRYYSQLGPWRQMLAIGLTTFAEKPEPTRSDCHAWSASPD
                     YDFLATICGIMPDAPGFDRVLVRPALGGLSEVEGSMPHPKGTIRVKLRKTGSGIEGEI
                     DLPPGVTGRFVYGGKAIALKGGSQAVRAGYPPPAITEGHREAGRGGVKADPVRVIFDS
                     DMGPDYDDVGAIALLHAFADSGKASILATMASNKYEGIAAVLNVFNTYFHRPDIPIGV
                     PRGQAVSQRDTQHWTDSILARYPHQVKRNEDAEDAVTLYRKILSRQPDRSVVIITVGF
                     LTNLKGLLESKPDVYSTLSGRDLVRRKVRQLVSMAGKFPQGMEFNVMKDAAASRAVFD
                     DWPTPILLSGFDIGVKIKCGLPLVRDASVRNDPVKDVFRISIPLAPEDSAGRSSWDET
                     AVLTGICGYAPWYSVKRGRLLVNPDGSDRWVDDKKGDRAQLVEAAPVRDVQDLINRLM
                     AHQPGAATTPSG"
     gene            complement(2904..>3213)
                     /locus_tag="VKU83_08595"
     CDS             complement(2904..>3213)
                     /locus_tag="VKU83_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93653.1"
                     /translation="YFAFQSGKWSMADVTVVAIFMAYIGFNGILESQMGYLNFRNDQL
                     SSIATNETSLQPGYIIFVTFVVFGLILSQILKMIIEKHGPPASRKELLRRRAGVPSGD
                     "
BASE COUNT          681 a          855 c         1044 g          633 t
ORIGIN      
        1 ggtgatccgg gacggcgctt atgttccgtg ctcaaccgac aatgggccgc ggttgcgctc
       61 gtatatggtc gtaggcccgg gcgacgaagt tgcgggcgag cggtatcctt ggggatggga
      121 acagccggca ttcaacgatg cggactggcc tgcggctgtg cccgtgtcga atccggcgct
      181 atggggattc ggctccgata atctttggac cctcgtgccg cggaccatcc cgctgatgga
      241 agagcgcttg cagcggatcc caagggttcg ccggtgggcg gaggaggcga atggaaaggg
      301 cggctccggt ggcggcaggg gcgagccgga agtggctgag gcccgtgtcg aacaaatgct
      361 ttcaggaaag ggcgcgttcg tcgtgcctgc gcatgaaagt ctgactattt tgcttgatca
      421 aacctataac accgtggcct atccggagtt acgggttagc ggaggtaagg gggccctggt
      481 ccggctgacc tacgcggagt cgttggtgga tagcctcgga gaaaagggcg accgcggggc
      541 gatcgaagga aaagtgatca agggtaatta cgatattttt aaacccgatg gcgggacagg
      601 caggttgttc cggcccctat ggttccgtac attccgctat ttgcagctcg aaatccggac
      661 gagcgacgac agcctccggg tcgacgacct gtatgggatg tacaccggtt acccttttgt
      721 gcgcaaggcg accttcacga gcagcgacac ctcgctgaaa tccatctggg atgtcggatg
      781 gcggacggcc aggctttgcg ctggagagac ctatttcgac tgcccttact acgagcaatt
      841 gcaatatgaa gccgataccc ggatccagtc cctcatctcc ttatacgtga ccggggacga
      901 ccggctgatg cggaaggcgt tgctggattt ctaccactcc cggatacccg agggattgac
      961 gcaagggaga tacccgagca gccgtttcca ggtgatcccg ccattctcgt tatggtggat
     1021 cgtaatgctc cacgattatt ggatgctgcg taaggacgat gccttcgtgg gccagttcct
     1081 gcccgcggtc aggggagtac tggattggtt cgaatcgcac atcgacacgt cgatggggat
     1141 gctggggccg atgccgtggt ggggatttgt tgactgggcg gatgcattcc ccggcggtga
     1201 tcccccgggg gcgaacaatg ggcattccgc ggtgatcagc ctgcaatatg cttatacgtt
     1261 agcccaggcg gcggagctct ttaaatattt tggcgggcaa tgggcgtatg aggggcagcg
     1321 atgcggacgg ttggcggcgg aactcaaccg cggcacgtac cgcgcctgct ttgacgcaag
     1381 ccgcggtgaa atggccaata cgcctcagaa gaacagcttc agccaacatg cgagcattct
     1441 ggcgatcctg tcaaatgcga tcccggagac cggacggcga cctctgatgg caaaggtact
     1501 cgcagacacc tccctcgaac aggcgacatt ctattttcgt ttttatctca atcaggcgat
     1561 gaaggcagcc gggatgggag accgctatta ctcgcagctg gggccctggc gtcaaatgct
     1621 ggcgataggc cttacgacat ttgcggaaaa gccggagcca acacggtcgg actgccacgc
     1681 gtggagcgca agcccggatt atgatttcct tgccaccatt tgcggcatta tgccggatgc
     1741 gcccggtttt gaccgggtat tggtccgtcc ggcgctgggc ggcctgagcg aggtggaggg
     1801 tagcatgccc catccaaagg ggacgatcag ggtgaaactg cggaagaccg gatcgggaat
     1861 agagggtgag atagacttgc cgcccggtgt caccgggcga tttgtctacg gcggcaaggc
     1921 catcgcgttg aagggcggaa gccaggcagt gcgagccggt tacccgccgc cggcaataac
     1981 cgaagggcat agggaagcgg gccgcggcgg cgtgaaggcg gacccggtgc gcgtgatctt
     2041 cgacagcgac atgggacctg actatgatga tgtcggcgcg attgcactgc tgcatgcttt
     2101 tgcggacagc ggcaaggcta gtatcctggc gacgatggcc agcaataaat acgaagggat
     2161 cgcggccgtg ctcaacgtgt tcaataccta ttttcaccgg cccgatattc ccatcggagt
     2221 gcccaggggg caggcggtca gccagcgcga tacgcagcat tggacggaca gcatcctcgc
     2281 ccggtatccc caccaggtca agcggaatga agatgccgag gatgcggtaa cgctataccg
     2341 aaaaatatta tcccgccagc cggaccgaag cgtcgtgatc attacggtgg gcttcctgac
     2401 caacttgaaa gggcttttgg agtccaagcc ggatgtctat tcaacgctga gcggccggga
     2461 ccttgtgagg cggaaggtca ggcagctggt ctccatggcc ggcaaattcc cacaaggcat
     2521 ggagttcaac gtaatgaagg atgctgcggc gtcccgtgcg gttttcgatg actggccaac
     2581 gcccattctg ctgagcgggt tcgacatcgg ggtgaagatc aaatgtggac taccactagt
     2641 gcgcgacgca tcggtccgca acgaccctgt caaggatgtg ttccgcatca gtatcccgct
     2701 tgcacctgaa gacagtgccg gcagaagcag ttgggacgag acggcggtat tgaccgggat
     2761 ctgcggctat gcgccatggt attcggtgaa acggggcaga ctgctcgtaa atcctgacgg
     2821 aagcgaccgc tgggtcgacg ataaaaaggg agatcgggca caattggtcg aggccgctcc
     2881 ggtgcgggat gtccaggacc tgatcaatcg cctgatggca caccagcccg gcgccgcaac
     2941 aactccttcc ggctagcagg cgggccatgt ttttcgatga tcattttcag gatctgggag
     3001 aggatgaggc caaagaccac aaaggtgacg aagatgatat agcccggctg cagggaagtc
     3061 tcgttcgtag caatgctgct caactggtcg ttccggaaat tgagataacc catctgcgat
     3121 tcgaggatgc cgttgaaccc gatataggcc atgaagatcg cgacgaccgt cacgtccgcc
     3181 atgctccact tgccggactg aaacgcaaaa tag
//
LOCUS       DATGKE010000499         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1018787, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000499 DATGKE010000000
VERSION     DATGKE010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018787"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VKU83_08600"
     CDS             complement(<1..602)
                     /locus_tag="VKU83_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93654.1"
                     /translation="MSWVFLTGEFAYKLKKPVKYRFLDLSILRSRLRNCREEVRLNKR
                     LARDIYLGLVPLTIEKNGRLALEGKGRVVDWLVRMQRLPEESMLDYGIRHHTVTKDQV
                     RPAAALLAEFYKSLPAIDTDPSRYREKLRGEIYSLYRELINPAYHLPVLLIDLLTAGL
                     LRFLTEHTSLLDNRALGGKIKETHGDLRPEHICLFPVPAI"
     gene            complement(743..1714)
                     /locus_tag="VKU83_08605"
     CDS             complement(743..1714)
                     /locus_tag="VKU83_08605"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HLI93655.1"
                     /translation="MILFALNSSRDFGEPVALELDMSLGEHEEVEFEDGENKCRPLEN
                     VRGRNVFVIQSLYSDKNKSVNDKLCRLLFFISALKDASAKHVTAIVPYLCYARKDRKT
                     KSRDPITTRYVAQLFEAAGTDHIVTVDVHNIQAFQNAFRIPSEHLEAKKVFADFLIPQ
                     IENDETIIMSPDIGGIKRAEQFQRLVSKRLHKDLQVAFMEKYRSSGEVWGETVVGDVK
                     GKTVVIIDDLVSTGGTLARSAAACKRQGAKKVIAVATHGIFTGQPDKTLGAEGLDQIV
                     ITNTIPPFRLENTQVKEKVKILSVAPLFAEAIRRLDEGGSITGLLED"
     gene            1902..3320
                     /locus_tag="VKU83_08610"
     CDS             1902..3320
                     /locus_tag="VKU83_08610"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HLI93656.1"
                     /translation="MVQFSISGSYTDLYELTMGEVYFLEGRKDDPANFDYFFRKIPNG
                     GGYVLFSGLHDLLEILETLQFTDADIKFLQNLHFRPAYIDFLRSFQFRGSVYAVREGE
                     VVFPNSPLLRVEGTLFETQLVETLLLNLLNFESLIATKASRMRQVAGNRLLSDFGLRR
                     AHGPGGVLAARAAIVGGFDSTSNVYAARLYDAETAGTMAHSFVESYDSEIEAFRAFAR
                     SRPDNCIFLIDTYNTLQSGLPNAIIVAKEMERAGHRAAGIRLDSGDLAWLSKNARALL
                     DREGLSYMKIVASNQLDEYVIRSLLEQGAPIDIFGVGTRLVTGQPDAALDGVYKLSMA
                     AGKPRLKLSESVQKITLPGVKQVMRVMKENGEFFGADAILLAGEEKPGMIYDPFEADK
                     SLAIGEYVREPLLQKVMGDGKRLAPAPTLDETAAYASGRLALLPQEYKRFENPHHYKT
                     GISKELMELRNDLRDQHKTGKK"
     gene            3317..3934
                     /gene="pncA"
                     /locus_tag="VKU83_08615"
     CDS             3317..3934
                     /gene="pncA"
                     /locus_tag="VKU83_08615"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HLI93657.1"
                     /translation="MNALILVDIQNDFLPGGPLAVPDGDAILPVVNDLQDHFGLVAAT
                     QDWHPLGHRSFASGHPGKEAFETVNLDGLPQTLWPDHCVQGTPGAGFPARLNMNKPEA
                     IFRKGTDPEMDSYSGFYDNGHRKSTGLTGYLRDRSVDTIYLAGLAGDFCIYYTAVDSL
                     KAAFSTFIIEDATRPLNRGNFEKVMLDFLARGGKLIQSGALAGIK"
BASE COUNT         1086 a         1126 c         1093 g         1075 t
ORIGIN      
        1 atgatagcag ggacgggaaa aaggcagata tgctccggcc ggaggtcgcc atgcgtctcc
       61 ttgatcttcc cgcccagcgc cctattatcc aacagggagg tatgttcggt aaggaaacgc
      121 aggagaccgg ctgtcaacag gtcgatcaat aatacaggca aatgataagc aggattgatc
      181 aattccctgt aaaggctata aatctccccc ctgagttttt ccctgtaccg gctgggatct
      241 gtgtcaatgg ccggcaagct cttatagaat tcagccagta aagccgcggc tggccttacc
      301 tggtctttcg tgacggtatg atgccggata ccatagtcca gcatgctttc ctccggcaat
      361 cgctgcatcc tgacaagcca gtccacgacc cttccttttc cctctaatgc caacctccca
      421 tttttttcga tggtaagagg cacgaggccc aggtatatat ccctggcaag ccgtttgttc
      481 agccggacct cctcccggca attcctgagc cttgatctca atatgctgag atccaggaag
      541 cggtatttaa caggcttttt caatttatac gcgaactcac ccgtcaggaa gacccatgac
      601 atatgcgtct cttttgtttc gacgctctga actggatgag gataagcaac ggcttgcttt
      661 aaaaaagcga ctttttcggc aagaggcaaa gcaatttccg gaggcggagc ggcgatgatc
      721 tttttcatca ttagcgtcct atttagtctt ccaacaaacc ggttatggac ccaccttcat
      781 ccagtcgtct gatcgcttca gcaaaaagtg gcgccacact caatatttta accttttcct
      841 tcacttgtgt attttccaac cggaacggcg gtatcgtatt cgttattaca atttgatcca
      901 ggccctccgc cccgagggtc ttatccggct gacccgtgaa gatgccgtgg gtagcaacag
      961 ctatgacctt tttagctcct tgccgcttac aggcagcggc tgaacgcgcg agtgttccac
     1021 ctgtgctcac caggtcatcg ataatgacca ctgtcttgcc tttcacatca ccgacaacgg
     1081 tttctcccca cacttccccg ctgctccggt atttctccat gaaagccacc tgcaggtctt
     1141 tatgcagacg tttgcttacc aatcgctgga attgttcagc tcgcttgatg ccgccaatat
     1201 cgggcgacat aataatggtt tcatcatttt ctatttgcgg gattaggaaa tcggcgaata
     1261 cttttttggc ttccagatgc tcggatggga tacgaaaggc gttttgaaaa gcctgaatat
     1321 tatgaacatc aacggtaaca atatggtctg tccccgctgc ttcgaaaagc tgcgccacat
     1381 aacgggtggt tatggggtcc cgcgactttg ttttccggtc ctttcgggca taacataaat
     1441 agggaactat ggctgtaaca tgcttcgccg aagcgtcttt tagggcggaa ataaaaaata
     1501 acaggcggca aagcttatca ttgacgcttt tgttcttatc actataaaga gattggatca
     1561 caaacacatt ccggccccgc acattctcca acggtctgca cttattttcg ccgtcctcaa
     1621 attcaacttc ctcatgttca cccagggaca tatccaactc cagggccacc ggttccccaa
     1681 aatcacgact gctgtttaac gcaaataata tcataaacga tcatttaacc aattacccaa
     1741 cagaggaaat caaataaaaa ttagtatata taacaacctt ttaaattgag ttgtatcagg
     1801 cgcgacgatg atcgtggtca cgctcccggc gattgtccgc tgcgttagat tcataataca
     1861 atcatgataa acaaaagcaa ggaatcagct acttttgaga aatggtacaa ttttccatca
     1921 gcggaagcta tacggatctc tacgagctaa cgatggggga agtgtatttc ctggaaggca
     1981 gaaaagatga ccccgccaat tttgattatt tcttccggaa gatacccaat ggcggcggat
     2041 acgtgttgtt ttcgggactg catgacctcc tggaaatcct ggaaaccctg caattcaccg
     2101 atgcggatat caagttcctc caaaatctgc atttccggcc ggcctatatc gatttcctgc
     2161 gaagctttca atttcgcggc tccgtatatg ccgttaggga aggcgaagtc gtatttccca
     2221 acagccccct gcttcgggtc gaggggaccc tgtttgaaac gcaactggta gaaaccttgc
     2281 tgctcaatct attgaacttc gaatcgctga tagccaccaa agcttcccgt atgcggcagg
     2341 tggcgggcaa ccgcctttta agtgattttg gcctgcgcag ggcgcatggc ccgggcggag
     2401 tgctggctgc gcgagccgcg attgtgggcg gttttgacag caccagcaat gtgtacgctg
     2461 ccaggctata tgatgcagag actgccggga ccatggcgca ttcttttgtt gaaagctatg
     2521 attcggagat agaggctttc cgcgcctttg ctcgctcacg tccggacaac tgcatattcc
     2581 tgatcgatac ctacaacacc ctccaaagcg ggttgcccaa tgccataata gtagcaaagg
     2641 aaatggaaag ggcgggacac cgggctgccg gcatccgtct tgacagtggc gacctggcct
     2701 ggctgtcaaa aaatgcccgg gccctgctgg acagggaagg tctctcctac atgaagatcg
     2761 tcgcatccaa ccaactggat gaatatgtga tcaggagcct gctggagcag ggagcgccca
     2821 tcgacatctt cggggtgggt acccggctgg taacgggcca gcccgatgca gccctggacg
     2881 gcgtatacaa gctgtctatg gctgccggta agccacggct gaaattatcc gaaagtgtac
     2941 aaaaaataac attgcccggc gttaaacagg ttatgcgggt catgaaggag aacggggagt
     3001 ttttcggggc cgatgcgatc ctgttagccg gagaagaaaa accggggatg atctatgatc
     3061 cgttcgaggc ggacaaatcg ctggccatcg gggaatatgt ccgggaaccg ttgctgcaaa
     3121 aggttatggg ggacggcaaa cggttggcgc cggcgcctac tttggatgaa acggccgcct
     3181 atgcctctgg gcggttagcg ttgttaccgc aagaatacaa gcggtttgaa aacccgcacc
     3241 attacaagac gggtatcagt aaagaactga tggagctgag gaatgacctc agagatcagc
     3301 ataaaacagg gaaaaaatga atgcccttat attggtggat atccagaacg attttttgcc
     3361 gggcggtccc ctggctgttc ccgatggtga tgccatcctg ccagtagtaa atgaccttca
     3421 ggatcacttc ggcctggttg ccgcgacgca ggactggcac ccgctcggtc accgaagttt
     3481 cgcctccggc cacccgggaa aagaagcatt cgaaaccgtc aacctggatg gtcttccaca
     3541 gaccctttgg ccggaccact gcgtacaagg taccccgggt gccggattcc ctgcccggct
     3601 taacatgaat aaaccggagg ccattttccg gaaaggcacc gatcccgaaa tggacagcta
     3661 cagtggtttc tatgacaacg gccatcgcaa gtcaacaggt ctgacagggt atcttcggga
     3721 tagaagcgtg gataccattt acctggcggg gctcgctggc gatttctgca tatattatac
     3781 cgccgtggat agcctgaagg cggccttttc cacctttatt atcgaagatg ccacccggcc
     3841 actcaaccgg gggaatttcg aaaaagttat gcttgatttc ctggcaagag gcggaaagct
     3901 catccaaagt ggagctctgg cgggaataaa atagaaaaaa ggcaggctcg aaagcctggc
     3961 ctccgccggc atctttccgc tggaagaaca ggatttgctt tttacaggct aagttctcct
     4021 ccatccggta tcggggtgcc gaatgacgcg gacagctttt tcattcgttg gatgcaccac
     4081 atccagctat cctcgtcagc gaggtttacc tggcacttcg agattgagag gggaataagc
     4141 tttaatttca ataatcttct attttctgca tacacgatga agaaaaaact acgatcaatc
     4201 cttaatatag ggtcgacggc atagtcgtcg atgagatcgc cggtgtcgta aagtataagc
     4261 ttgttcctgt gaacttctat tccctgaaag atatgagcac tatggccgtg aatgatatcg
     4321 gcgccctgat cgatcatttc atgcgccagg tctacaaaga caggatgagg cttctcctat
//
LOCUS       DATGKE010000500         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_416989, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000500 DATGKE010000000
VERSION     DATGKE010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416989"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1988)
                     /locus_tag="VKU83_08620"
     CDS             complement(12..1988)
                     /locus_tag="VKU83_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HLI93658.1"
                     /translation="MLAGLQNVTFEFGARVIVKDATWHIQPNERIGLIGYNGTGKSTL
                     LKLFAGEYVPSAGSVERSRNTTIGYLHQDLLSFDTKGSILEVAMTAFERVLQLEKEIE
                     MLGKQLEKDHENEALLHQYSDALHEMDTLDGYTIQNRTEEILQGLGFANSDLGRPFRD
                     FSGGWRMRVLLARMILQKPDLLLLDEPTNHMDLPSIEWLEKYLQHYQGSVVIVSHDKY
                     FLNRMVTKIVELYQKELHIYNGNYTFYETEKAQRVELQQRAFENQQEYIRQQERFIER
                     FRAKATKAAAAQSAMKRLEKIERIEDVEIERPNIRINFRIDKTPGRVLATLRHATKRF
                     GPITIVENASAEIDRGDKIALIGANGKGKSTLLRMIAGTEPFSGERIWGHNVEESFYA
                     QHQLEALDVNNTVLEEMQLCGSQKTDLELRVLLGCFLFSGDDVEKKIKVLSGGEKARV
                     ALAKTIVSKANFLLLDEPTNHLDMHSCELLIEALNKYEGSYLLVSHDRYFISKTANKI
                     WEIDNHQIREFKGSYEEWVDWKERNVAARQQAAVAAPTAAPVSFPTSGPVQRNRGEDV
                     SSAAKASPINREAKKELQRQQRLFQDIEQKIAGLTRQKAQLEASLAEPSTYSDKDKFL
                     KAEAEYKKATDELEKVNRQYEEVFDKIVELEKTL"
     gene            2051..>2528
                     /locus_tag="VKU83_08625"
     CDS             2051..>2528
                     /locus_tag="VKU83_08625"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HLI93659.1"
                     /translation="MRKFVLLVVLFIVAGLLVYRLLSDKVTNQPPPPDLALHISKNSS
                     SFNMAFAGLMDQYFDMKDALVNWDTAKADAAAYRVAAKADSLPVGRLKADSAIVKTAE
                     SLAASIDAEAKGFTAETGIEGRRRSFNMLTDELYNLIRTVRYDGQTVYHIRCPMAFG"
BASE COUNT          482 a          693 c          707 g          646 t
ORIGIN      
        1 gtttcatttg gtcaaagcgt cttctccaac tcgacgatct tgtcaaaaac ttcctcatat
       61 tggcggttga ctttctccag ctcgtccgtg gcctttttgt attcggcctc agcctttagg
      121 aatttatcct tatcggaata cgtagaaggt tcggcgaggg atgcttccag ctgggccttt
      181 tggcgggtga ggccagcaat cttctgttcg atgtcctgga acagacgttg ctggcgctgg
      241 agctccttct ttgcctctct gttgatgggt gacgccttcg ccgctgatga aacgtcttcg
      301 ccacggtttc gctgcaccgg gccggaggtt ggaaaggaca ccggggcggc tgtgggggca
      361 gcaaccgccg cctgctgcct ggccgcgaca ttccgctctt tccaatcaac ccattcctca
      421 tagctgcctt tgaattcgcg gatctggtgg ttgtcgatct cccagatctt gttggccgtt
      481 ttcgaaatga aataacggtc gtggctgacc agcaggtagc tgccttcata cttattcagc
      541 gcctcaatca ggagttcgca ggagtgcatg tcaaggtggt tggtcggctc gtcgaggagc
      601 aggaaattcg ctttgctgac gatcgtcttg gcgagcgcca cacgcgcctt ctccccgccg
      661 ctgagcactt tgatcttctt ctcaacgtcg tcgccactaa aaaggaaaca gccgagtaga
      721 acgcgcagtt cgagatcggt cttctggcta ccgcaaagct gcatttcctc gagcaccgta
      781 ttgttcacgt ccagcgcttc cagctgatgc tgcgcgtaaa agctttcctc gacattgtga
      841 ccccagatcc gttcgccgct gaacggttcc gtgccggcga tcatccgcag cagtgtcgat
      901 ttacctttcc cgttagcccc gatgagggcg atcttatcac cgcggtcgat ctcggcggac
      961 gcattctcga cgatggtgat cggcccaaat ctttttgtgg catggcgcag cgtggccagg
     1021 acccggcccg gcgtcttgtc aattcgaaaa ttgatgcgga tattgggccg ctcgatctcg
     1081 acgtcttcaa tgcgctcgat cttttcgagg cgcttcatgg cggactgggc ggccgccgcc
     1141 ttggtggcct tggcgcggaa ccgttcgatg aatctttcct gttggcggat gtattcctgc
     1201 tggttttcga aagcgcgttg ctggagttcc acccgctgcg ccttttcagt ctcgtagaag
     1261 gtataatttc cgttatagat atgaagctct ttctggtaca gctccacgat cttggtcacc
     1321 atccggttga ggaaatactt gtcatggctt acgatcacaa cggagccctg gtaatgctgg
     1381 aggtactttt cgagccattc aatcgaaggt aggtccatgt ggttcgtcgg ttcatccagc
     1441 aggaggaggt caggtttttg caggatcatc cgggccagga gtacgcgcat ccgccacccg
     1501 ccgctgaagt cccggaaagg acggcccagg tcgctgttgg caaagcccaa cccctggagg
     1561 atctcctcgg tccggttctg gatggtgtat ccgtcgaggg tatccatctc atgcagggcg
     1621 tcgctgtact ggtgcaacag cgcttcattc tcatgatcct tttcaagttg tttgcccagc
     1681 atctcgatct ctttttccag ctggaggacc ctttcaaagg cggtcattgc cacctccagg
     1741 atggatccct tcgtatcgaa gctgagcaga tcctggtgca ggtaaccaat cgtggtattc
     1801 cggctgcgct cgacactgcc tgccgagggc acgtattcgc cggcgaacag tttgagtaaa
     1861 gtcgatttgc cggtgccgtt gtagccgatg agaccgatgc gttcattggg ttgaatatgc
     1921 caggtcgcat cctttacgat taccctggcg ccgaattcga aagtgacgtt ctgaagtcct
     1981 gctaacatga attttaaaaa atggtatgca aatttagccc agtgctgtac cttcggcaaa
     2041 actttctttc atgcggaaat tcgtcttgct cgtcgtgctt tttattgtgg ctggcctcct
     2101 cgtgtacagg ctgttgtcgg acaaggtgac gaaccagccg ccgccaccgg accttgcgct
     2161 gcatatcagc aaaaacagca gttcgttcaa catggccttt gcgggcctga tggaccagta
     2221 tttcgacatg aaggacgccc tcgtcaattg ggataccgca aaagcggacg cggccgcgta
     2281 tcgggtggcc gcaaaggcgg acagcctgcc cgttggacgg ctgaaggcgg acagcgccat
     2341 cgtcaagact gccgaaagcc ttgccgcaag cattgacgcg gaggcgaagg gttttacggc
     2401 ggaaacgggc atcgagggcc ggcgtaggtc tttcaacatg ctcacggatg aattgtataa
     2461 cctcatccgt acggttcgat atgatgggca aaccgtctat cacatccggt gtccgatggc
     2521 attcgggg
//
LOCUS       DATGKE010000501         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_323966, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000501 DATGKE010000000
VERSION     DATGKE010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323966"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VKU83_08630"
     CDS             complement(<1..529)
                     /locus_tag="VKU83_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HLI93660.1"
                     /translation="MRNNHEIDYKIYGEELQFVEIELDPGETVMGEPGGFMMMDDNIQ
                     METIFGDGSAASQQGGLLGRLMNAGKRVLVGENLFMTAFTNTGVGKRRVSFAAPYTGK
                     IVPLDLQALGGTIIAQKDAFLCAAKGVSIGIAFQRRLGTGIFGGEGFIMERLDGDGMA
                     FVHAGGYIVEKELQPG"
     gene            complement(598..>2730)
                     /locus_tag="VKU83_08635"
     CDS             complement(598..>2730)
                     /locus_tag="VKU83_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI93661.1"
                     /translation="PAMLIFTLAASLVVAFIMNPVFAVDFMNHPEDGTTRTRAAIFKR
                     PGLWIAIAFGILLDLAGQRFLGNFLFFVVALVILNKFIFNPMIRGFQDRFLPSIMRQY
                     ERLLRWALQGWRPAWLLFSTFLLLIVSVIFFGARKVDVVFFPKGQPNQIYVYLKLPVG
                     TNVDYTDSVTHSLERRVFSVLGMGDGTKPNPIVESVIANVAVGANDPTSGDQSTHPEL
                     GRIQVSFVEYKKRHGVSTSMYLDSIRHVMKNIPGAEITVDQEQNGPPTDPPVNIEVAS
                     DNFDDLIRTAVALKNYLDSIQTPGVEELKMDIDLTNPEITLTVDRQRAMIEGINSSQI
                     GQELRTALFGLESSKIKEGKDEYKIYIRNLESQRKSLTDLLNMNITYRDIAAGGAIRN
                     VPISSLVHVDYTSTLGSVKRKNQKRTITLKSNVLNGYTPTAVNQDLAKSIAAFTHKPD
                     DVTITQTGEGQQQAETGAFLFKALIIALATILIILVIQFGSVSKSVIILNEILFSVIG
                     VIIGFAITKMQVSVVMTGLGIVGLAGIVVKNGILVIEFTDELRARGMKTREAAIEAGK
                     TRIIPVMLTAMAAILALIPLAVGFNINFITLFSDLNPQIYFGGDNAVFWKPLSWTIIF
                     GLAFAFFMTLFMVPSMYLIAERLRRPMRRHWGGKWVSFLGIPPLTILFLLLVFISMFR
                     HSSEVAARRRRAAIAKRRVDEKWIRSWF"
BASE COUNT          604 a          759 c          774 g          593 t
ORIGIN      
        1 cacccggctg aagctccttt tcgacgatat agccgccggc gtggacgaac gccatgccat
       61 cgccgtcgag ccgttccata ataaatccct cgccgccgaa aatcccggtc ccgagccgcc
      121 gctggaaggc gatgccgatg ctgacgccct tggcggcgca gagaaaggca tctttttgag
      181 cgatgatggt gccgcccagc gcttgcaggt cgagggggac gatcttgccg gtatatggtg
      241 cggcaaagct gacccggcgt ttgcccacgc cggtattcgt gaaggcggtc atgaaaaggt
      301 tctcgccgac aaggacgcgt ttcccggcat tcatcagccg gccaagcagg ccgccttgct
      361 gggaagctgc agaaccgtcg ccaaagatgg tttccatctg aatgttgtcg tccatcatca
      421 taaagccgcc gggttcgccc atgacggtct cgccggggtc cagttcgatc tcgacgaact
      481 gaagctcctc gccgtagatc ttgtagtcga tttcgtgatt gttgcgcata ggtataattt
      541 tatactttta gattctccaa tgcctgcgcc tgcggcgcag cgaccctgcc cctttgatca
      601 gaaccagctc ctgatccact tctcgtcaac ccggcgttta gcgatcgctg ctctgcgcct
      661 tcttgcagca acctcgctgc tatgccggaa catcgaaatg aacaccagca gtagaaaaag
      721 aatcgtaagg ggtggtattc ccaggaacga aacccatttt ccgccccagt gacgccgcat
      781 cggccgccgt agcctttccg cgatcaggta catactcggg accatgaaaa gggtcataaa
      841 gaacgcaaat gccagcccga agatgattgt ccaggacagc ggcttccaga acacggcatt
      901 gtcgccgccg aaatagatct gtgggttgag atccgaaaac agcgtaataa aattgatatt
      961 gaaacccact gccagcggaa tcagcgcgag gatcgcggcc atggccgtca gcatgaccgg
     1021 aatgatacgg gtcttgcccg cttcgatcgc ggcttcgcgg gtcttcatcc cgcgggcccg
     1081 cagctcgtcg gtgaattcta tcacgaggat gccattcttt accacgatgc cggcgaggcc
     1141 gacgataccc aaacccgtca tgaccaccga cacctgcatt ttggtaatgg caaacccaat
     1201 gatgacccca atgacgctga agaggatctc attgaggatg atcaccgatt tactgacgga
     1261 gccgaactgg ataacgagaa tgatcaggat ggtcgccagg gcgatgatca gcgctttgaa
     1321 caggaaggcg cccgtctcag cctgttgctg tccctcaccg gtttgagtga tcgtgacatc
     1381 atccggctta tgggtgaagg cagcaatcga cttggccaga tcctggttga ctgctgtcgg
     1441 cgtatagcca ttgagcacat ttgacttcag ggtaatcgtc cgcttttgat tcttgcgctt
     1501 gacgctgccg agcgtgctgg tatagtcaac atggacgagg gaactgatcg gaacattcct
     1561 gatcgccccg ccggctgcga tatcccggta cgtgatattc atgttaagca ggtccgtcag
     1621 gctcttgcgt tgcgactcga ggttacggat atagatcttg tattcatcct tcccttcctt
     1681 gatcttcgag gattccagcc cgaaaagcgc cgtccggagc tcctgcccga tctgcgagga
     1741 attgatcccc tcgatcattg cccgctggcg gtcgaccgtc agcgtgattt ccgggttagt
     1801 gaggtcgata tccattttga gctcctccac tccgggggtc tggatggaat caaggtaatt
     1861 tttcaatgcc accgcggttc ggatgaggtc gtcgaaatta tcgctggcga cctcgatatt
     1921 gacgggcgga tccgtgggcg gcccgttttg ttcctggtcg acggtgatct cggcgcccgg
     1981 gatatttttc atcacatggc ggatggaatc caggtacatg gaagtggaca cgccatggcg
     2041 tttcttgtat tcgacaaaag acacctggat gcggccgagt tccgggtgcg tgctttggtc
     2101 gccgctggtc gggtcattgg cgccgaccgc aacgttggcg atgacgcttt cgacgatggg
     2161 attgggtttc gtgccgtcac ccatgcccaa tacggaaaac acccgccttt ccagactgtg
     2221 ggtcacggaa tcggtatagt ccacatttgt accgaccggc agcttcagat agacgtagat
     2281 ctggtttggc tgacctttcg ggaagaaaac cacatccacc ttccgtgcgc caaaaaagat
     2341 cacggaaacg atgagcagca ggaaggtgct gaacaggagc caggccggcc gccagccctg
     2401 gagggcccac cgcagcaagc gttcatactg ccgcatgatc gaggggagga acctgtcctg
     2461 gaacccgcgg atcattgggt tgaagataaa tttgttgagg ataaccagcg ccacgacgaa
     2521 gaagagaaaa ttgcccagga accgctggcc ggcgagatca aggagaatgc caaaggcgat
     2581 cgcgatccaa agaccgggcc ttttgaaaat ggcagcgcgt gttcttgtcg tgccatcctc
     2641 aggatggttc atgaaatcca cggcaaagac gggattcatg ataaaagcca cgaccaggga
     2701 ggctgccagc gtaaagatca gcatcgccgg
//
LOCUS       DATGKE010000502         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1353639, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000502 DATGKE010000000
VERSION     DATGKE010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353639"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VKU83_08640"
     CDS             <1..395
                     /locus_tag="VKU83_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93662.1"
                     /translation="IPLAARACIGNLNLYAAGANFSSNGADLSAFGCDLTQWTTLRVI
                     SSHRHVDFFVNGKCAYSLSFPHDPTDIVGVQYRFSGTCAVKYARFTRGSTTADTPAKG
                     GTAIRSTPTGSAPASDSPKAVPRTINLN"
     gene            complement(410..1444)
                     /locus_tag="VKU83_08645"
     CDS             complement(410..1444)
                     /locus_tag="VKU83_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI93663.1"
                     /translation="MFTTANGVIFILYSETTSAMKNIAILVPQTAVIEAVADPRYAFT
                     AVNEFLKSSGEMPLFHVQLVGQTNDVRVGDGLFTIHADATLATARKPDLIIIPALSGD
                     LGQALQVNRDFIPWIVRHYKEGTEVASLCLGAFLLAETGLLNGKTCSTHWLFANDFRA
                     RFPGVDLLDDKIVTDQLGLYTSGGANSYWNLLLYLIEKYTNRELAVLTAKFFVLDIDR
                     NSQSPFHIFKGQKVHNDPEIVKVQEFIESNYSDKITVDELCRKFGIARRTFERRFKKA
                     THNTVLEYWQRVKIEAAKRQLEMSRKTVGEVMYEVGYNDTKAFRDIFRKVTSMSPVDY
                     RNKYNKEVLN"
     gene            1520..1930
                     /locus_tag="VKU83_08650"
     CDS             1520..1930
                     /locus_tag="VKU83_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI93664.1"
                     /translation="MTQINPYLNFNGQCREALTFYQQCLEADLQLQKVSESPMAAKMA
                     SEKGPLILHGALSKAGKTLLMGSDMIGENLRPGNSVNLCLHCTSDQEIDAFFNRLSAG
                     GQVKVPLHESFWGATFGEIKDKFGVTWMLNYTRN"
     gene            2013..2471
                     /locus_tag="VKU83_08655"
     CDS             2013..2471
                     /locus_tag="VKU83_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI93665.1"
                     /translation="MQITTRPATPGDLPTLLRFEQGVIAAERPLDPTIQDGPIHYYDL
                     NLMLNSPNIHLVVAEAGGTVVGSGYARIDPSRHYLKHSHHAYCGFMYVEPAWRGRGVN
                     QAIIAALRDWAQSRNLTELRLDVYTTNDAAIRAYEKAGFTPYLINMRLGL"
BASE COUNT          579 a          852 c          599 g          549 t
ORIGIN      
        1 ttatcccgct cgccgccaga gcctgcatcg gcaacctcaa cctctacgcc gcaggagcaa
       61 attttagcag caatggagcg gacctctccg cgttcggctg cgacctcacc caatggacca
      121 ccctgcgtgt catcagcagc caccgccacg tcgacttctt cgtcaatggg aagtgtgcgt
      181 attcgctttc gttccctcac gatcccacgg atatcgtcgg cgtgcaatac cgcttctccg
      241 gcacttgcgc cgtcaaatac gcccgcttca cccgtggcag cactacggcc gacaccccgg
      301 cgaaaggcgg cacggccatc aggagcacgc ccaccggcag cgcgcctgcc agcgatagcc
      361 ccaaagccgt cccccgcacc ataaatttga attaaacccc tgcgtaacct tagtttagca
      421 cctctttgtt atacttattc cggtaatcca ccggcgacat ggacgtcacc tttcgaaaga
      481 tatccctgaa ggccttcgtg tcattgtacc cgacctcata catcacttcg cccaccgttt
      541 tccggctcat ctccaactgc cgcttcgccg cctcgatctt gacccgctgc cagtattcca
      601 gcaccgtatt atgcgtggcc ttcttaaagc ggcgttcgaa ggtccgccgg gcaatcccaa
      661 actttctgca taactcatca accgtgatct tgtcggaata attactctcg ataaattcct
      721 gcaccttgac gatctccggg tcgttgtgca ctttctgacc cttgaaaata tggaaaggtg
      781 actggctgtt ccggtcaata tccaggacaa aaaactttgc cgtcagcact gccagctccc
      841 ggttggtgta tttctcaata aggtataaca ggagattcca ataagaattg gcgccaccgc
      901 tcgtatacag acccaactgg tccgtcacaa tcttatcatc caggaggtcc acgccgggga
      961 accgggccct gaagtcgttg gcgaacagcc agtgcgtcga gcaggttttg ccattcagca
     1021 aacctgtttc cgccaacaga aaggcgccca ggcaaagact cgccacctcg gtaccctctt
     1081 tataatgccg gactatccac gggataaagt cccggttcac ttgcaaggcc tggcccaaat
     1141 ccccgctcag ggcgggaatg atgatgaggt ccggttttct tgccgtcgcc agcgtggcgt
     1201 cggcatggat cgtaaacaac ccatccccga cccggacgtc gttcgtctgt cccaccagct
     1261 ggacgtggaa gaggggcatt tccccggaac tcttcagaaa ttcattcacg gccgtaaaag
     1321 catagcgcgg atcggccacc gcttcaatga ctgccgtttg cggcacaaga atggcaatat
     1381 ttttcatggc gctagtcgtt tcggaataga ggataaagat aacgccattt gctgtcgtaa
     1441 acaacccctt caattgacgg aaccgtacag ccccgctgcc cggaaagccc catagcttta
     1501 ccgcataaaa gcgtcctcca tgacacagat aaatccctac ttgaacttca atggccagtg
     1561 ccgagaagcg ctaacttttt accagcagtg cctcgaagct gatctccagc ttcaaaaggt
     1621 ctcggaatcg ccgatggccg caaagatggc ctccgaaaaa ggacccctga ttctgcacgg
     1681 cgcactctct aaagccggca aaaccctcct catgggatca gacatgatcg gggaaaacct
     1741 gcgtcccggg aactccgtga acctctgcct ccattgcacc agcgaccagg agatcgacgc
     1801 cttcttcaac cgcctctccg ccggcgggca ggtcaaggtt cccctccacg aatccttctg
     1861 gggcgccacc ttcggcgaaa tcaaagacaa attcggcgtc acctggatgc tcaactatac
     1921 gcgcaactga aggaacttat ggaatctgct gctgccaact gtcgctcgcc cggccagccc
     1981 ccccgctccg ctattctccg taacttcatt ccatgcaaat cactacgcgc cctgcgacgc
     2041 ccggcgacct gccaaccctg cttcgctttg aacaaggcgt catcgccgcc gaacgcccgt
     2101 tggaccccac catccaggac ggccctatcc attactacga tcttaatttg atgcttaatt
     2161 cgccgaacat ccatctcgtc gtcgcggagg ccggcggcac ggtcgttggc tcgggttacg
     2221 cgcggatcga cccctctcgc cattacctga aacacagcca ccacgcatac tgcggcttca
     2281 tgtacgtcga acccgcctgg cggggcaggg gtgtcaacca ggcgatcatc gccgcacttc
     2341 gcgactgggc ccaatcgcgc aaccttaccg aactgcgcct cgacgtctac actaccaatg
     2401 acgccgccat ccgcgcctat gaaaaggccg gatttacacc ttacctgatc aatatgcgcc
     2461 tggggctgta gcggaaaagg ctgaagcgta attagaacgc tattccttta cgaactcata
     2521 cggatcgatt tccacaaaat cactttgctt cgtcttcagg ccaaccacct tcccgccgg
//
LOCUS       DATGKE010000503         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_772316, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000503 DATGKE010000000
VERSION     DATGKE010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772316"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="VKU83_08660"
     CDS             <1..1562
                     /locus_tag="VKU83_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI93666.1"
                     /translation="CDQAATDRQDHIWAVTRSDSLMELEPDTSPGKSDLRLIAAWAKI
                     AQSPRSLCVDGAGRVWIGTRDRGLYCLEMDGMRVKALHQLTIKDGLSENFVRYLYCDP
                     DNVIWAATPSGLDRVSLRKDGFFVTNMTAGQGLGVLKVCQSADRTIWAVADGGFLQIS
                     PAAPAPNGYRPGVLFSQVLVGNQQVPGEAQSPMVLSHDRNTVSFNVGAPSFTDENQTR
                     FSYLLEGGSEAGWSPPTRQSAINFVNLPPSKYTLWVRAKFLSGAYPDAVASYPFLIRP
                     PWWQTTTFRIAGFIVLLFVIGWAIRSYTRSRLEAQRITLEKEQAIEKERTRIATDMHD
                     DLGAGLSRIKFLSDTIGIKQQRRMSIDEEIDGIREYSKEMIDKMGEIVWALNQKHDLL
                     SDLLSYTRSYAAAYLMQAAIQTRIEAPEEFPYIVVSGEFRRNVYLAVKEALHNIAKHS
                     QAQQVTIRMEVGKALVIRLKDDGVGFDRARIRPYANGLVNMEKRIRDLGGDLIVDTGA
                     GGTTVTIVVPV"
     gene            1615..2349
                     /locus_tag="VKU83_08665"
     CDS             1615..2349
                     /locus_tag="VKU83_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI93667.1"
                     /translation="MPISLAIIEDLDEVRDGLKNFLALSTEFDVLACFKTAEDALAAS
                     TSCRPDIFIVDINLPGMNGIEFVRRASKEDGGAQFMMFTVYENDEKVFEALKAGASGY
                     LLKNTGLVHIIEALKELHEGGSPMSAAIARKLVRAFQDRSGSRTLRGGGAAGPGQGTA
                     TVLAPSAGSGIMGQDTGNSGPDCWDSALSARENEILQLLARGLLYKEISDQLHISTST
                     VRQHIHRIYEKLHVQNRTEAINKTKK"
     gene            2420..>2962
                     /locus_tag="VKU83_08670"
     CDS             2420..>2962
                     /locus_tag="VKU83_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI93668.1"
                     /translation="MKRFAGLLLLLLPLTIFAQQYSYHPDSTRPQKQPHRSIGIGVMA
                     GFNFANVTNASSINASTRAGYHFGLFLAPDSKRILGSRTELLYSRHGYNYKTDTGGGA
                     VNLDYIMLTQMMAFHITKYFELDLGGYTAYLLAAKDDSMSKALAGTPYAGYSSLLSFY
                     NRFDYGFGVGAEVHPVLGVLI"
BASE COUNT          638 a          822 c          873 g          629 t
ORIGIN      
        1 tttgcgacca ggccgccacc gaccggcagg atcacatttg ggcggttacc cgctcggaca
       61 gtctgatgga gctggaaccg gataccagcc ccggcaaaag cgatctgcgg ctgattgccg
      121 cctgggcgaa gatcgcgcaa agtccaaggt cgctatgcgt cgatggtgcg gggcgggttt
      181 ggatcggcac gcgcgaccgg ggcctttatt gtctggaaat ggacgggatg cgggtcaaag
      241 ctttgcatca gctgacgatc aaggatggac tctcggaaaa ttttgtccgg tacctttact
      301 gcgatcccga caacgtgatc tgggctgcga cgccttcggg gctcgaccgg gtgtccctgc
      361 gtaaagatgg cttcttcgtg accaatatga cggccggcca ggggcttggt gtcctcaaag
      421 tttgtcagtc ggcggaccgg acgatttggg cggtcgctga cgggggcttc ctgcagatca
      481 gcccggccgc gccggcccca aatggctacc ggccgggcgt acttttttcc caggtcctgg
      541 tggggaacca gcaagtcccc ggcgaagcgc aatcacccat ggtcttgagc cacgaccgga
      601 acactgtctc cttcaatgtc ggtgcgccca gtttcaccga tgaaaatcag acccggttca
      661 gttacctgct cgagggcgga agcgaagccg gatggagtcc gcccacccgg cagtcggcaa
      721 tcaattttgt gaacctgccg ccatcgaaat atacgctctg ggtaagggcg aaattccttt
      781 ccggcgccta ccccgacgcc gtcgcctcct atcccttcct gatccggcca ccctggtggc
      841 aaaccacgac cttccggatc gcgggtttca tcgtcttgct cttcgtgatc ggctgggcga
      901 tccgcagtta tacccgcagc cggctcgagg cccagcgcat caccctggaa aaagaacagg
      961 ccatcgaaaa agaaaggacc aggatcgcga ccgacatgca tgacgacctg ggagccgggc
     1021 tttcgaggat caagttcctg agcgacacga tcggcatcaa gcagcagcga cggatgtcca
     1081 ttgacgaaga gatcgacggc atccgcgaat attcgaagga aatgatcgat aaaatgggcg
     1141 agatcgtttg ggcgctgaac caaaagcacg acctcctgag cgacctgcta tcctataccc
     1201 gctcttatgc cgccgcctac ctgatgcagg cggcaatcca aacgcgtatc gaggcgccgg
     1261 aggaatttcc ctatatcgta gtaagcggcg aattcaggag aaacgtctac ctcgccgtga
     1321 aggaagcttt gcataatatc gccaagcatt cgcaagccca gcaggtaacg atccggatgg
     1381 aggtgggcaa ggcgttggtg atccgcctga aggacgacgg cgttggattt gaccgcgcgc
     1441 ggatccggcc ttatgcgaat ggcctcgtga acatggaaaa gcgtatcagg gacctgggtg
     1501 gagatcttat cgtggatacc ggggccggcg gcacgacggt gacgatcgtg gttcctgtat
     1561 aacttttgta ctattgcgag ggggacccat tcatagtagt tttatttccg tcacatgccc
     1621 attagcttag cgatcataga ggacctcgac gaggtgcggg acggcctgaa gaatttcctg
     1681 gcccttagca ccgagtttga tgtcctggca tgcttcaaga ccgcggagga tgcgcttgcg
     1741 gcttcgacct cgtgcaggcc ggacattttt attgtcgata ttaacctgcc cggaatgaac
     1801 gggatcgagt ttgtccggcg agccagcaag gaggacgggg gagcgcaatt catgatgttt
     1861 accgtatacg agaatgacga gaaggtcttc gaggcattga aggcgggagc ctccggctat
     1921 ctgctgaaaa acaccggtct ggtgcacatc atcgaggcct taaaggaatt gcacgaaggc
     1981 gggtccccga tgagtgcggc gattgcccgc aagctggtca gggcattcca ggatcggagc
     2041 ggcagtcgaa cgctgcgggg cggcggggcg gcggggcccg gtcagggaac cgcaacggtc
     2101 cttgccccat cggcaggctc cggcattatg ggccaggata ccgggaattc cggtccggac
     2161 tgctgggatt cggccttgtc ggcgcgggaa aatgagatct tgcaattgct ggcgaggggc
     2221 cttctttaca aagagatttc cgatcagctg catatttcca ccagcacggt caggcagcat
     2281 attcaccgca tctatgagaa gctccacgtg cagaaccgta cggaagccat taacaaaacg
     2341 aagaaataac ttttgttcta ttgacccggg accgggcagg aattagatta gcaccgataa
     2401 ccagataaaa ttttcgctta tgaaacgatt tgcaggtctt cttttgctgc ttcttccgct
     2461 tactatcttc gcgcagcagt attcgtatca tccggattcg acgcgtccgc aaaagcagcc
     2521 gcaccggtcg atcgggatcg gcgtcatggc aggcttcaat ttcgccaacg tgaccaatgc
     2581 ctcatccatc aacgccagca cccgcgccgg atatcacttc gggctcttcc tggctccgga
     2641 ctccaaacgt atcctcggat cgcggaccga attgctgtat tcccggcatg gatacaacta
     2701 caagaccgat accggtggcg gtgccgtcaa cctggactat atcatgttga cccagatgat
     2761 ggcgtttcat atcaccaaat attttgagct tgacctgggt ggatatacgg cctacctttt
     2821 agccgccaaa gacgactcga tgtcaaaggc ccttgccggt actccctatg ccggctattc
     2881 gtcgttgctg agtttctata accgcttcga ttacgggttt ggcgtcggcg cggaggtcca
     2941 cccggtgttg ggagtgctga tc
//
LOCUS       DATGKE010000504         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1242317, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000504 DATGKE010000000
VERSION     DATGKE010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242317"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VKU83_08675"
     CDS             <1..681
                     /locus_tag="VKU83_08675"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HLI93669.1"
                     /translation="LKELKSVLGSTPSMDGLELASVVSTDQEYALGDPASSIRVAVLD
                     YGVKKHILQCMVERGAYVMVFPAKTPLSRLQEFQPSGYFLSNGPGDPAPMEYAIPTVR
                     AILKEKKPLFGICLGHQLLALANDIPTFKMHHGHRGLNHPVKNLVTGRCEITTQNHGF
                     GVDPQAVRNAANVEVTHINLNDQSIEGIRLKDRPAFSVQYHPEATPGPHDSRYLFDDF
                     IQLIKNNQ"
     gene            690..1103
                     /gene="aroQ"
                     /locus_tag="VKU83_08680"
     CDS             690..1103
                     /gene="aroQ"
                     /locus_tag="VKU83_08680"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLI93670.1"
                     /translation="MRIAIINGPNLNLLGTREPGIYGSQTFGEYYDDLRKKYPSVEFS
                     YFQSNIEGELVDELQRAGFSADGIILNPGGYTHTSVAIGDAIAAIKAPVIEVHISNIH
                     AREEFRRLSHVSGKVKGTIAGLGLKGYQLAVEYFL"
     gene            complement(1108..1758)
                     /locus_tag="VKU83_08685"
     CDS             complement(1108..1758)
                     /locus_tag="VKU83_08685"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLI93671.1"
                     /translation="MKIYLGLALILMTAGTAAAQVTPAPMSVRQMKAELEKASNPLLY
                     ARDVLKKRYRLDTVTVTQTRHFGGLADSLAYNGKLRKVYGPYGPKNGRFLVQVLSKAP
                     NEFYHISQIFIDTSVFSYKFADSLGNSILKKLRDGSATFEDMARTYALGGQAAAMGDM
                     GWIARGAMFPVVEHEVITHKKGEVFKLWNRAGLNIIRKDDDPRKDTGFALMMQVFL"
     gene            1827..>2204
                     /locus_tag="VKU83_08690"
     CDS             1827..>2204
                     /locus_tag="VKU83_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93672.1"
                     /translation="MHRFIFLLIPALLGQLCGQAQWKSYIIGVKGDTLNCVDQQDRKQ
                     GRWSIHVDELRGEPGYDEEGVFKDNRKEGVWRIYDLDGDLTGLEYYKWGNKNGVCQYF
                     ATNGALIREESWIALNPDKVYDTL"
BASE COUNT          526 a          564 c          598 g          516 t
ORIGIN      
        1 ctgaaagaat taaaatctgt tttgggttcc acgccgtcga tggacgggtt ggagcttgcg
       61 tcggtggtaa gcacggacca ggaatacgca ctcggcgatc cggcgtcatc aatccgcgtc
      121 gcggtgctgg attatggggt caagaagcac atcctgcaat gcatggtgga aagaggcgcc
      181 tacgtcatgg tgtttccggc aaaaacgccg ctgtcccggc tgcaggagtt tcaacccagc
      241 ggttatttcc tgtccaatgg tcccggcgat ccggcgccca tggaatatgc tatcccgaca
      301 gttagggcaa tattgaagga gaagaaaccg ttgttcggga tatgtctggg tcaccaactg
      361 ctggcactcg ccaatgacat cccaaccttt aaaatgcatc atggccaccg gggattgaac
      421 cacccggtaa agaacctcgt gacggggcgc tgcgagatca cgacccagaa tcacggattt
      481 ggggtcgacc cgcaggcagt gcgaaacgcc gccaacgtgg aagtcactca catcaacctg
      541 aatgatcagt cgattgaagg catcaggctg aaagaccgcc ccgctttttc agttcaatac
      601 catcccgaag caacaccggg ccctcatgac agccgttatc ttttcgatga tttcattcaa
      661 ttgatcaaaa acaatcagta agcctgacca tgcgcatcgc cattatcaac ggcccgaatc
      721 tgaacctctt gggtacccgg gagcctggaa tatatggcag ccagacattc ggggagtatt
      781 acgacgactt acggaaaaaa tacccttcgg tggaattcag ttactttcaa agcaatattg
      841 aaggggaact ggtcgacgaa ctgcaacgtg ccgggttttc cgcagacggc attatcctga
      901 atccgggcgg ctacacgcat acctcggtcg caatcggcga tgccatcgcc gccatcaaag
      961 cgccggtcat cgaagtgcat atcagtaaca tccatgcgag ggaggaattt cgtcgcttgt
     1021 cccatgtttc agggaaggtg aagggcacta ttgccgggct tgggttgaag ggctatcaac
     1081 tcgccgtgga atatttcctg tagctgatca gagaaacacc tgcatcatta aagcgaatcc
     1141 cgtatccttt cgggggtcgt cgtccttacg gatgatattt agccctgccc tgttccacaa
     1201 cttgaagact tctcctttct tgtgcgtaat gacctcgtgt tcgacgactg gaaacattgc
     1261 gccgcgtgcg atccagccca tgtcgcccat cgcggcagcc tggccgccca gggcatacgt
     1321 ccttgccatg tcctcaaatg tggcggagcc gtccctcagc tttttcagga tgctgtttcc
     1381 aagggaatcg gcgaacttat agctgaagac ggaggtgtcg ataaaaatct ggctgatgtg
     1441 gtaaaattca ttcggcgcct tgctgagtac ctgtaccaga aagcggccat ttttcggccc
     1501 gtaaggacca taaacctttc ttagctttcc attataggcc aggctgtcag cgaggccgcc
     1561 gaaatgccgc gtttgggtga ccgtgaccgt gtcgagcctg tatcgttttt tcaggacgtc
     1621 cctggcatac aataggggat tggacgcttt ttccaattcc gctttcattt gcctgaccga
     1681 catcggcgcc ggcgtcacct gggctgcggc cgttcccgcc gtcatcagga ttagggcaag
     1741 tccaagatat attttcatgt caatcagtta agtatgcaaa tatagggcaa ccaatatttt
     1801 ggaatgattt tcgttatttg aagaacatgc atcgtttcat ttttctcctt attcctgccc
     1861 tgctcggcca gctgtgcggg caggcgcaat ggaagtctta tattatcggc gtaaagggcg
     1921 ataccctgaa ctgcgtcgat cagcaggacc ggaagcaggg ccgctggtca attcatgtag
     1981 acgagctgcg gggcgagccg ggatatgacg aggagggggt ctttaaggac aacaggaagg
     2041 aaggagtgtg gcggatctac gacctggatg gcgacctgac gggactggaa tactacaaat
     2101 ggggcaataa aaatggtgtt tgccaatatt tcgcgaccaa tggagccctg atcagagaag
     2161 agagctggat agcgctcaat ccggataagg tttatgacac actg
//
LOCUS       DATGKE010000505         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1539657, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000505 DATGKE010000000
VERSION     DATGKE010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539657"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..359
                     /locus_tag="VKU83_08695"
     CDS             45..359
                     /locus_tag="VKU83_08695"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLI93673.1"
                     /translation="MKARRDVFQAIADPTRRAILALLTAQALTPGVITASFDVSQQTI
                     SKHIQILAECELVTREHRGREIYYELNPKKLKEIADWLKPFQQMWENRFSKLDKILKR
                     PK"
     gene            361..1065
                     /locus_tag="VKU83_08700"
     CDS             361..1065
                     /locus_tag="VKU83_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HLI93674.1"
                     /translation="MKNLLSARLSIAAAIAYQLLLVVLIFLRPDLPVYSTTISEWAIG
                     HYGWLMQVAFWISASSYLFLYATLKTEVKGRPGKIGLALLFICFLGTFGVGVFTTNPY
                     PPDLRIPKTLIHTCCGTLAMILYPVAILLIALDISRRNEAWAGYRSILKAIGFLPLLA
                     FAGFIIHLNLYVIPLGKGAVGPDVPVGYPPRVMFLAYHLTLIAMATLFLKKQTKHEIK
                     WQPQKSLLKKANKSFS"
     gene            1014..1493
                     /locus_tag="VKU83_08705"
     CDS             1014..1493
                     /locus_tag="VKU83_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLI93675.1"
                     /translation="MATTEIIAEKGKQEFFIEREFDAPRDRVFEAFSNPETLAEFFAP
                     FDLTMEFNYHDYRSRGSYSWCNKKGNKTVCTFAGVIHELTAPLRIVQTSEFMELPERG
                     NVVLEIIQFDALPDNRTKVTIHDICPSTAVRDSIINSGMEKGVVDIFNKLDKYFGAK"
     gene            complement(1525..2352)
                     /locus_tag="VKU83_08710"
     CDS             complement(1525..2352)
                     /locus_tag="VKU83_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93676.1"
                     /translation="MKRGVILSLTCAMVTVLPACVKHLDIGEIVQHDIVKSCPILQMW
                     SVDPSKPDTTGAEVVVHYNFAGNPVEMLNIKGSGYRQDFHFRYDRSNRLTDFLMNPSI
                     SPVAPAALEWHTYTYPSRRMVVDSVYGENDPAGITAPRPNADAFAGVVTHFLDDEGRI
                     IKDEGNWYGAYTDTSYFQYDAWGDMVRPGVMYDNKYNPYLTNDVWRFVYEDYSVNNPL
                     QGTIGPPLIIFQYDARGLPQLLGGFGDVWLFRYEFAPGLQIIYGCDQGQGNTVSKAE"
     gene            complement(2377..3201)
                     /locus_tag="VKU83_08715"
     CDS             complement(2377..3201)
                     /locus_tag="VKU83_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93677.1"
                     /translation="MKRRLLLLLTCAMVMALPACVKRLDLGQRVQEEVKSCPILQLAC
                     VVRLNSHDTTEIEIAVHYNAVGNPIAMLHINGAGTLDLRDYHFRYDRYNRLSDYIMNS
                     SGTYNALMWHTYTYTSRNVVVDSVYGENDPAPITARKPNADSYIGVTRHLLDNEGRII
                     EDQQYWYGGSLKDTTYFQYDAWGNIIRSRVDYDNKFNPYLTNDVWRFVYEDYSVNNPL
                     PENSGTPLLIAAYNAHGLPQILGYLAGTVHLFGYEFPAEMQIIYGCDNGQKENGVE"
     gene            complement(3333..>3844)
                     /locus_tag="VKU83_08720"
     CDS             complement(3333..>3844)
                     /locus_tag="VKU83_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLI93678.1"
                     /translation="KHSTMEHETKITRNPGEKKLLVIREFNAPVEKVWQAFTKQETLD
                     LWWAPRPWKTETKSLDFEPGGLWLYSMVGPNGDRHHCRVEFTEIIPQRSFNASSGFCD
                     ENGVPNPSLPLMHWHTLFTSTSTGSKIDVTIRFDKEADLKTIVEMGFEAGFTMALGNL
                     DELLEGVTA"
BASE COUNT          941 a          968 c          994 g          941 t
ORIGIN      
        1 cagtcaaata actgtacata catttacagt caaatgactg tataatgaag gcaagaaggg
       61 atgtatttca ggctatcgcc gaccccacca ggagggcaat tttggcgctg ctgacggcgc
      121 aagccctgac cccgggcgtg attacggcca gtttcgacgt atcacagcag accatctcaa
      181 agcatattca aattctggcg gaatgcgaac tagttacccg ggaacacagg ggaagagaga
      241 tctactatga attaaacccc aaaaagttga aggagatcgc cgattggctg aagccgttcc
      301 aacaaatgtg ggaaaaccgt ttttccaagc tggataaaat cttaaaacga ccaaaataaa
      361 atgaaaaatc tactatcggc aaggctatcc attgcagcgg caattgccta ccagctgcta
      421 ttggttgtac tcatcttcct gcggccggat ttgccggttt actccactac catcagtgaa
      481 tgggccattg gacattatgg atggctgatg caagtggcgt tttggatatc cgcaagcagc
      541 tatctctttc tctatgcgac actcaaaacc gaggtgaagg gaaggccggg gaaaatcgga
      601 ttagcgctgc tctttatctg cttcctcggc acttttggcg tcggcgtctt tactaccaat
      661 ccatacccgc ctgatttgcg aattccaaag accttgatac atacgtgttg cggaacgctg
      721 gcgatgattc tttacccggt tgccatcctc ctgattgcgc tggatatctc cagacggaac
      781 gaggcttggg ctggttacag gtcgatcctt aaagccatcg gcttcctacc gcttttggca
      841 ttcgcgggct ttatcattca cctgaatttg tatgtcattc cgctgggcaa aggcgccgtg
      901 ggcccggatg tgccggtggg ctatccgccc agggtgatgt ttctggccta tcatctgacg
      961 ctgatagcga tggcaacatt gttcttaaaa aaacaaacaa agcacgaaat aaaatggcaa
     1021 ccacagaaat cattgctgaa aaaggcaaac aagagttttt catagaaagg gaatttgatg
     1081 cgccgcggga tcgggtattt gaagcgtttt ccaacccgga aacgctggcc gaattctttg
     1141 cgcccttcga cctgacgatg gagttcaatt atcacgacta ccggtcgcgc gggtcctata
     1201 gctggtgcaa taaaaaaggg aacaaaacgg tctgcacgtt cgccggggtt attcacgagc
     1261 tgaccgcgcc gctgcgcatc gtccagacgt ccgaattcat ggaattgccg gaaagaggga
     1321 atgtggtttt ggaaataata caattcgatg cattgcctga caatcggaca aaggtcacca
     1381 ttcatgacat ctgcccgagc accgccgtaa gggattcgat cattaacagc ggtatggaaa
     1441 agggcgttgt cgatatcttt aataagctgg ataaatactt cggggccaaa taaaagcgat
     1501 aacccaaggt tgtttgctta accgctattc cgccttactc acagtgttac cctggccctg
     1561 atcgcagcca tagatgattt gcaatcccgg agcaaactcg tagcggaaaa gccagacatc
     1621 gccaaagcca cccaggagct ggggcaggcc acgagcatca tactgaaaaa taatcaaggg
     1681 cggcccgata gttccctgca atggattgtt cacactgtag tcctcataaa cgaatctcca
     1741 gacatcattg gtgaggtagg gattgtactt gttgtcatac attacgccgg gccggaccat
     1801 atcgccccag gcatcatatt ggaaatagct ggtatctgtg taggccccgt accaattccc
     1861 ctcatctttg ataatccttc cttcgtcatc caggaaatga gtgaccacgc cggcgaacgc
     1921 gtcggcattt ggtcttggcg cggtaatccc cgccgggtca ttttcgccat acacggaatc
     1981 cacgaccatc cggcgggagg gataagtata ggtatgccat tccaatgcgg caggtgctac
     2041 aggcgatatg ctcgggttca tcagaaagtc ggtaagccgg ttggaccggt cataccggaa
     2101 atgaaaatcc tgccggtacc cgctgccctt tatattcaac atttcgactg ggttgccggc
     2161 gaaattataa tggaccacga cctcggcgcc cgttgtatcg ggttttgacg gatcaaccga
     2221 ccacatttgc aggatcggac aggacttgac aatatcgtgc tggacaattt cgccaatgtc
     2281 caggtgtttt acacaggccg gcaaaaccgt aaccatcgcg caggtcaaag ataaaatcac
     2341 gccccttttc atatcgatta gtttaaatga tgcccgtcac tctaccccat tctccttttg
     2401 cccgttatca caaccgtaga ttatttgcat ttcagcagga aattcataac cgaaaaggtg
     2461 gacagtacct gcgaggtatc caaggatctg gggcagaccg tgagcgttat aggctgcaat
     2521 caacaacggc gtgccactat tttcaggcaa cggattgtta acactatagt cctcgtaaac
     2581 gaatctccag acatcgttgg tgagataggg gttgaacttg ttgtcataat cgacgcggga
     2641 ccggatgatg ttgccccagg cgtcatactg gaaataggtg gtgtctttta gggacccgcc
     2701 ataccaatat tgctgatctt caatgatcct tccttcgtta tccaggagat gccgcgtcac
     2761 gccaatatag ctgtcggcat tcggtttccg ggcggtgatc ggcgccgggt cattttcgcc
     2821 atacactgaa tccacgacca catttcgcga cgtataggta taggtgtgcc acatcaacgc
     2881 attgtaggtc ccgctcgagt tcatgatata gtcgcttagc cggttgtatc gatcataccg
     2941 gaaatgatag tcccgcaagt ccagcgttcc ggcgccattt atatgcaaca tcgctatcgg
     3001 gttgccgacg gcattgtaat ggacagcgat ctcgatctcc gtggtatcgt ggctattcaa
     3061 tctgaccaca catgcgagtt gcaggatcgg gcaggacttg acttcttcct ggacgcgttg
     3121 tcccaggtcg aggcgtttga cacaggccgg caaggccata accatggcac aggttaataa
     3181 aagcagcagt cgccttttca tttaaaaaga tttagaaagc ccgaatgctg caatattacc
     3241 gggcggacgt ggtaataaaa atgaccgggg tcaggctggc atatgacggt gctctccgcc
     3301 ctgcggcgaa gcggcgttcc gcccctggcg ccttacgcgg ttacgccttc cagtaactca
     3361 tccaggttcc ccagcgccat cgtgaacccg gcctcgaagc ccatttcgac gatggttttc
     3421 agatcggcct ccttgtcgaa gcggatggtc acatcgatct ttgagcccgt ggaagtggaa
     3481 gtgaacaggg tgtgccagtg catgaggggg aggctggggt tggggacgcc gttttcgtcg
     3541 caaaagccgc tgcttgcatt aaaactgcgc tgcgggatga tttcggtgaa ctcgacgcgg
     3601 caatggtgtc gatcgccgtt gggcccgacc atgctatata gccagaggcc gccgggttcg
     3661 aagtcgaggg atttggtctc ggtcttccag gggcggggag cccaccatag gtcaagggtt
     3721 tcttgcttgg tgaaagcctg ccatactttc tcaactgggg cgttgaattc ccggatgacg
     3781 agcaattttt tttctccggg attcctggtg atcttggttt catgttccat agttgaatgt
     3841 ttta
//
LOCUS       DATGKE010000506         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_121994, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000506 DATGKE010000000
VERSION     DATGKE010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121994"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VKU83_08725"
     CDS             complement(<1..1046)
                     /locus_tag="VKU83_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLI93679.1"
                     /translation="MKIALAQQNYHIGNFEENIRKILEGIARAKDAGADLVVFSELSV
                     CGYPPRDFLEFDDFIARCYEAVDRIREYADTIGVIIGSPVRNPVREGKDLFNGAWLLY
                     EKEIKGVAHKTCLPNYDVFDEYRYFEPGYQWKVIPFKGKKIALTVCEDIWNLGDNPLY
                     RECPMDHLIRQHPDLMINISASPFDYDHDEDRKEVVRQNVLKYHLPMYYCNAVGSQTE
                     IVYDGGSVVFDAKGNLIRELAYFEEDFDVVDLPHPVPPLYEELLPGLSVAINRNEDSI
                     HPAPQPVSTPEHIFDKDMRVSKQNDPGRILQYLTGERNIRQIHKALILGIRDYFHKMG
                     LKKAVIASSGGVDSA"
     gene            1083..1442
                     /locus_tag="VKU83_08730"
     CDS             1083..1442
                     /locus_tag="VKU83_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HLI93680.1"
                     /translation="MAFNEQLASRIREALAHLPHVEEKKMFHGICFMVNGKMCLCVRL
                     DEMLCRVGPHKYGGALEMSHCRPMIHNGRTMTGFVFVSEEGIRRKKDFEYWVDLALEF
                     NKEAKPSKKTVKVKKKK"
     gene            1439..>2229
                     /locus_tag="VKU83_08735"
     CDS             1439..>2229
                     /locus_tag="VKU83_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93681.1"
                     /translation="MIQRILPLLALFFLFACNHPEDRPDVSGVHVDNIHIVRFDTAFF
                     GLDSNHIEAGLFALNRRYPFFTQDFVANILGAEPLSDTSRLAFEASRQFLITYHPVRD
                     SLEPKFQDLRWLEKQLQLGFRYVKYYFPAYRLPPKVVAFIGPFDGPGVAITAYTLAIG
                     LQSYGGKDFSFYLTGKGQDMYPLYISRRFEPKYITLNCLNAIAQDIFPDSSDGRPLIE
                     QMVTKGKYLWLAGKLLPDAPDSITTGYTQSQLNWSSANEAAIWNF"
BASE COUNT          532 a          565 c          584 g          548 t
ORIGIN      
        1 gcgctgtcga cgccgccgct gctggcgata acggcctttt taagccccat cttgtgaaaa
       61 tagtcccgga tgcccaggat aagggccttg tgtatctggc ggatattgcg ttccccggtg
      121 aggtattgaa ggatccgccc gggatcattc tgtttggata cgcgcatatc cttatcaaaa
      181 atgtgttcgg gtgtggatac gggctgcggc gccggatgaa tgctatcttc attgcggtta
      241 atggctaccg agaggcccgg aagcaactct tcataaagcg gcggcacggg atgaggcaga
      301 tccaccacat cgaaatcctc ctcaaaataa gcgagctctc tgatcaggtt ccctttcgca
      361 tcaaagacta cgcttccgcc gtcgtatacg atctccgttt gtgaaccgac ggcattgcag
      421 taatacatgg gaagatgata tttgagcacg ttctgtctga cgacctcttt acgatcctca
      481 tcatgatcat agtcgaaggg tgaagcggaa atattgatca tgagatcggg atgctggcgg
      541 atgaggtgat ccatgggaca ttccctgtac agcggattat cgcccaggtt ccagatatcc
      601 tcgcaaacag taagagcgat ctttttgcct ttgaatggaa tgaccttcca ttggtaaccc
      661 ggctcgaaat aacgatattc atcgaagaca tcgtagttgg gcagacaggt cttgtgggcc
      721 actcctttta tttctttttc ataaagaagc caggcgccgt taaagaggtc ctttccttcg
      781 cgaacgggat tacgcaccgg cgaaccgatg atgacaccga tcgtatcggc atattcacgg
      841 atccggtcta ccgcttcgta acacctggcg ataaaatcgt cgaactccag gaagtcacgc
      901 ggtggatagc cgcataccga tagctcggag aataccacca gatccgcgcc tgcgtctttc
      961 gcgcgggcga tgccttccag gatcttacgg atattttcct caaaattgcc gatatgataa
     1021 ttctgttggg cgagtgcgat cttcatacca caaagttaaa gttatagagt tatatttggg
     1081 ctatggcttt caacgaacag ctggcgagcc ggataagaga ggcgctggcc cacttaccgc
     1141 atgtggaaga gaaaaagatg ttccatggca tctgttttat ggtgaacggc aagatgtgtc
     1201 tttgtgtgcg gctggatgag atgctctgcc gcgtcgggcc tcacaagtac gggggtgcgc
     1261 tggaaatgag ccattgcaga ccgatgatcc acaacggccg caccatgacg gggtttgtgt
     1321 tcgtcagcga agaaggcatc cgcagaaaaa aggattttga atattgggtg gatctggccc
     1381 tggagttcaa caaagaggct aaaccttcaa aaaaaactgt taaagttaaa aagaagaaat
     1441 gatccaacgt atcctgccgc ttctcgccct tttttttctt ttcgcctgta atcatccgga
     1501 ggatcgaccc gatgtctcgg gcgtccatgt ggacaacata catatagtac ggttcgatac
     1561 cgcttttttc gggctggact ccaatcatat cgaagcgggg ctgttcgcgc ttaatcggcg
     1621 atatcccttt tttacccagg actttgtagc caatatcctg ggagccgagc cgctatccga
     1681 cacgagccgc ctggcgtttg aagcctcccg gcagttcctg ataacctatc accccgtccg
     1741 ggattcgctc gaaccgaaat tccaggacct gcgatggctg gagaaacagc tgcaactggg
     1801 tttccgttac gtaaagtatt atttcccggc ttaccggcta ccaccgaaag tagtggcttt
     1861 tataggtccg ttcgacgggc ccggcgtggc cattaccgcc tacacgctgg ccatagggct
     1921 gcagagctat ggtggcaagg atttcagctt ctatctcacg ggcaaaggac aggacatgta
     1981 ccctctctac atctccagac ggttcgagcc gaagtatatc acgttgaatt gtctgaatgc
     2041 gatcgcacag gatatcttcc ccgatagcag cgatggccgg ccgcttatag aacagatggt
     2101 caccaagggc aaatatctat ggctggccgg aaagctgctg cccgatgctc cggacagcat
     2161 tactaccggc tacacgcagt ctcagctgaa ttggagctcg gctaacgaag cggccatctg
     2221 gaatttctt
//
LOCUS       DATGKE010000507         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_6907, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000507 DATGKE010000000
VERSION     DATGKE010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6907"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VKU83_08740"
     CDS             <1..1788
                     /locus_tag="VKU83_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLI93682.1"
                     /translation="CFTGAYAIHPFNGLHLPVWISEYVLAGYGTGAIMAVPCGDERDF
                     RFARHFQLNITNIIGDAFTGKEANPTKDAVLENSDFLDGLVMKDAILIAIRRLEEKGI
                     GTRKVNYKMRDAAFSRQRYWGEPFPITWENGIAVPLDETALPLELPHIDKYGPGPEGE
                     GPLANLPAWMERRLETNTMPGYAGSSWYFLRYMDPHNDAAFCSRAASDYWGQVDLYIG
                     GAEHAVGHLLYSRMWTKFLYDRGWIGFDEPFKRLINQGMIQGSSRLVYRLETRGEVEE
                     LTSQRPVFVSAGLALAGSEPEVHEVLAIALRGIFNDPLLILRDIAFTPLHVDVNMVDG
                     TELDIEAFRKWRAEYAHAEFILEDGKYLCGVEVEKMSKRLYNTVNPNDLVKKYGADAY
                     RMYEMFLGPVEQSKPWDTKGIEGVFRFLRKLWRLFFDETKGRVWTTEKPSDAEWKSIY
                     RTIRRTEDATERFSFNTGVSALMIGVNELTDLKSHKKEVLGKLVVVLAPYAPHLAEEL
                     WHLLGNPGSVLDAAYPEVIEKWLVDSAKVYPVAINGKTRTELNIALDATQQQVEEIVL
                     TDETVRKWLNGNLPKKIVYVKNRMINVVV"
     gene            1795..>2253
                     /locus_tag="VKU83_08745"
     CDS             1795..>2253
                     /locus_tag="VKU83_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLI93683.1"
                     /translation="MDSKTDFSSVVTRQQTGFHPVVPFDPAKDRLHGFDFTEANTDLA
                     PADIADTESFAAYINRALQRDRARYGIGGYGEHRTLYARSRHFGAAGAEEPRRLHLGI
                     DIWGPAGTPVMAPLDGIVHSFGFNNNDSDYGATIILTHNLEGSTFHTLYGH"
BASE COUNT          501 a          615 c          676 g          461 t
ORIGIN      
        1 tgtttcaccg gcgcgtatgc catccatccg ttcaacggtc tgcacctccc ggtctggatc
       61 tcggaatatg tactggccgg ctatgggacc ggcgccatca tggccgtccc ctgcggcgac
      121 gaacgggact tccggttcgc ccggcatttt cagcttaaca ttaccaacat catcggggat
      181 gccttcacgg ggaaggaggc caaccctaca aaagatgccg tgctggagaa cagcgatttt
      241 ctcgacgggc tggtcatgaa ggatgcgatc ctgatcgcga tccgccgcct ggaggaaaag
      301 ggcatcggaa cgcgaaaggt caattacaag atgcgggatg ccgcattcag ccgtcaaagg
      361 tattggggcg agcccttccc gataacctgg gaaaacggca ttgccgtccc gctggatgaa
      421 acggcgcttc cgcttgaatt gccgcatatc gacaaatacg ggcccgggcc cgagggcgag
      481 ggtccgcttg ccaacctgcc ggcgtggatg gagcgaaggc tcgagacgaa caccatgccg
      541 ggttatgccg gtagcagttg gtattttctc cgctatatgg atccgcacaa cgatgcagcg
      601 ttttgcagcc gcgcggcctc ggattattgg ggccaggtgg atctttacat tggcggcgcc
      661 gagcacgcgg tgggccacct gctgtatagc aggatgtgga caaaattcct ttacgatcgc
      721 ggctggatcg gcttcgacga gccttttaag cgcctgatca atcagggcat gatccagggc
      781 agcagccggc tcgtgtaccg cctcgaaacg cggggcgaag tggaggaact gaccagccag
      841 cggccggtgt tcgtctcggc gggtctggcg ctcgcgggct ccgaaccgga ggtccacgaa
      901 gtcctggcga ttgcgctccg cggtattttc aacgaccctt tgctgatcct tcgcgacatt
      961 gcatttacgc cacttcatgt cgatgtcaac atggtcgatg ggacagagct cgacatcgag
     1021 gccttccgga aatggcgcgc ggagtacgcg cacgccgaat ttatcctcga agacggaaaa
     1081 tatctttgcg gcgttgaggt cgaaaaaatg agtaagcggc tgtacaatac cgttaatcca
     1141 aacgacctcg taaaaaagta tggggccgac gcttaccgga tgtacgaaat gttcctgggc
     1201 ccggtcgagc aaagcaaacc atgggatacg aagggaattg aaggtgtgtt cagattcctc
     1261 cgcaagctgt ggcggctttt ctttgacgag acgaagggta gggtctggac gacggagaaa
     1321 ccctcggatg ctgaatggaa atccatttac cggacgatcc ggcggacgga agatgccacc
     1381 gaacgttttt cgttcaacac cggagtcagc gcactcatga tcggtgttaa cgagctgact
     1441 gatctgaaaa gccataaaaa ggaagtgctc gggaagctgg tggtcgtcct cgcgccctat
     1501 gcgccacacc tggcggagga actttggcac ctcctcggga atcccggttc agttctcgat
     1561 gctgcttatc ccgaagttat tgaaaaatgg ctcgtcgatt ccgcgaaggt ctacccggtg
     1621 gcgatcaacg ggaaaacaag aacggaactc aatatcgccc tggatgccac tcagcagcag
     1681 gtggaggaga tcgtactgac cgacgagacg gtcaggaagt ggcttaacgg gaatttgccg
     1741 aaaaagatcg tgtatgtcaa gaaccggatg atcaacgtcg ttgtataaga agatatggac
     1801 tcaaaaacgg acttctcgtc agtggtgacc cggcaacaga ccgggtttca tccggtggtg
     1861 ccgtttgatc cggcaaaaga ccggttgcac ggctttgatt tcaccgaagc caataccgac
     1921 ctcgctccgg cagatattgc ggataccgag tcgtttgccg cttatatcaa ccgggcgctc
     1981 cagcgggatc gcgcccgcta tggcatcggc ggctatggcg agcatcggac gctgtatgcg
     2041 cggagccggc atttcggcgc cgcaggggct gaagagccgc ggcgccttca cctgggcatc
     2101 gatatctggg gccctgccgg cacgccggtc atggcgccgc tggacggcat tgtccatagc
     2161 tttggcttca ataataacga cagcgactac ggggcgacga tcatcctcac ccacaacctt
     2221 gagggttcta cattccacac cttgtatggc cat
//
LOCUS       DATGKE010000508         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_589602, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000508 DATGKE010000000
VERSION     DATGKE010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589602"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /gene="feoB"
                     /locus_tag="VKU83_08750"
     CDS             <1..2133
                     /gene="feoB"
                     /locus_tag="VKU83_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HLI93684.1"
                     /translation="AFALKNKNLHIALIGNPNSGKSSLFNALTGLNQKVGNFPGVTVD
                     KKSGTCTLAQDLQATVTDLPGTYSLYPKRADEWVTYKVLLNQDPAVRADMVILLVDAS
                     NLKRNLLFCSQIIDLKIPVVVALTMMDLAAKKGIEIDVEGLERELGIPIVVVNPRKNK
                     GIPQLKKIVDLRMRQVRQAPRRDFIPNQQLAADAIRDVRQLFPDISDYTAIHYLMNHE
                     HFSLTPQLQHKVEQIEAAHHFNHTRIQADEILQRYGRIKQIMHHTVAEPDPLERTLFT
                     ERLDKILLDRTWGYIILLGVLFLLFQSVFWFAQFPMDGIDWTFSQLGGWLGRHLPAVW
                     WSNLFVNGLLAGLSGILVFVPQIMILFGLITILEDTGYMARISFLSDKLMRKVGLNGK
                     SVMPMISGFACAVPAIMSARNIENRKERLLTILITPLMSCSARLPVYTMLIALVIPRK
                     DVFGIFSLQGLVMMGLYMLGLVMAMIVSYVAKWFIRLKEKSFFILELPIYRAPRWNNV
                     IVTMINKAKIFVLDAGKVIMVISLILWALSSFGPAHRMSAVSAAYAAQEEQHPARAAE
                     LDREKHSALLENSYAGILGKVIQPVIRPLGYDWKIGIAVITSFAAREVFVGTMATLYS
                     VEGGKDADQETLRQRMQSARREDGTPVYTLASGVSLMIFYVLAMQCMSTLAVVRRETR
                     SWKWPAIQVVYMTGLAYLASLIAYQLLK"
     gene            complement(2134..>2528)
                     /locus_tag="VKU83_08755"
     CDS             complement(2134..>2528)
                     /locus_tag="VKU83_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLI93685.1"
                     /translation="IRAYYGRWQEMLGGPIHETVEIFRELKFHTHLKLYALTNWSAET
                     FPVALELYDFLHWFDGRLVSGEEKIRKPFPEIYRLLIGRYGIEPRKSIYVDDNIRNVL
                     PARELGFYGIQFRTPELFRAELKGLGVL"
BASE COUNT          542 a          776 c          704 g          506 t
ORIGIN      
        1 gcattcgcct tgaagaacaa aaacctccat attgccctca tcggcaaccc caatagcgga
       61 aaaagctcgc ttttcaatgc cctgaccggc ctgaaccaga aagtcgggaa tttccccggc
      121 gtcaccgtcg acaaaaagag cggaacctgt accctcgccc aagacctcca ggccacggtt
      181 accgacctgc cgggcactta cagcctgtat ccgaaaaggg cggacgagtg ggtgacctat
      241 aaagtcctgc tgaaccagga cccggccgtc cgcgccgaca tggtgatcct tttggtggac
      301 gccagcaacc tgaaacggaa cctgctcttc tgctcccaga tcatcgacct gaagatcccc
      361 gtggttgtcg ccctcacgat gatggatctc gcggcaaaaa aaggcatcga gatcgacgtg
      421 gaaggtttgg agcgggagct ggggatcccg atcgtcgtcg tcaatccccg aaagaataaa
      481 ggcatcccgc agctgaaaaa gatcgtcgac ctgcgcatgc gccaggtccg gcaggcgccg
      541 cgccgggact ttatcccgaa ccagcagctt gccgcggatg ccatccggga cgtcaggcag
      601 ctctttccgg atatcagcga ttataccgcg atccactacc tgatgaacca cgaacatttc
      661 agtctgacgc cgcagctgca gcacaaggtg gaacaaatcg aagccgccca ccatttcaac
      721 cacacgcgca tccaggcgga cgagatcctc cagcgctatg gccgcatcaa gcagatcatg
      781 caccacaccg ttgccgagcc cgatccgctc gagcgaaccc tatttaccga acgactggac
      841 aagatcctgc tcgaccgcac ctgggggtat attatcctgc tgggcgtcct cttcctcctt
      901 tttcaaagtg tattctggtt cgcgcagttc ccgatggacg gcatcgactg gacgttctcg
      961 cagctcggcg gctggcttgg ccgccacctt ccggcggtgt ggtggagcaa ccttttcgtc
     1021 aacgggttgc tggcggggct tagcggaata cttgtttttg ttccgcaaat catgatcctc
     1081 ttcgggctga tcaccatcct cgaagacacc ggctatatgg cccgcatcag cttcctgagc
     1141 gacaagctca tgcggaaagt gggattgaac gggaagagcg tcatgcccat gatcagcgga
     1201 tttgcctgcg cggtcccggc gatcatgagc gcccgcaaca tcgagaaccg caaggaacgg
     1261 ctgctgacca tcctgatcac cccgctgatg agctgcagcg cccgccttcc cgtgtacacg
     1321 atgctgatcg ccctggtcat cccgcgaaag gacgttttcg ggatattcag cctgcaggga
     1381 ctggtgatga tgggattata tatgctgggc ctggtcatgg ccatgatcgt cagctatgtc
     1441 gcaaaatggt tcatccggct gaaggagaaa agctttttca tcctcgaact gccgatctac
     1501 cgggcgccgc gctggaataa cgtcatcgtg accatgatca acaaggcaaa gatctttgtg
     1561 ctcgatgccg gtaaggtcat catggtgatc agcctgatct tgtgggcatt gagctctttc
     1621 ggtccggccc accgcatgag cgcggtttcc gcggcatacg ccgcccagga agagcagcat
     1681 ccggcccggg ctgccgaact cgaccgggag aagcactcgg ctctgctgga gaattcttac
     1741 gccggcatcc tcggcaaggt catccagccg gttatccgcc ccctcgggta cgactggaag
     1801 atcggcattg ccgtgatcac ctccttcgcg gcgcgggaag tattcgtcgg cacgatggca
     1861 acgctttaca gcgtcgaagg cggaaaggac gccgaccagg aaaccctccg gcagcggatg
     1921 cagtcggcgc gccgcgagga cggcacgccc gtctatacgc ttgcgagcgg ggtttcgctg
     1981 atgatctttt acgtcctcgc catgcaatgc atgagcacgc tggcagtcgt ccggcgcgag
     2041 acccgaagtt ggaaatggcc cgccatccag gtggtctata tgaccgggct cgcttatctc
     2101 gccagcctga tcgcgtatca gctgctgaaa tagttacagc accccaagcc ccttcagttc
     2161 cgccctgaaa agttccggcg tccgaaactg gatgccataa aaacccagtt cccgggcagg
     2221 caggacattg cggatattgt cgtcgacata gatcgatttg cggggttcga tgccgtaccg
     2281 cccgatcaat aagcggtaga tctcggggaa gggtttccgg atcttctctt cgccggacac
     2341 gagccggcca tcaaaccaat gcaggaaatc gtagagctcg agcgccaccg gaaaggtttc
     2401 cgcgctccag ttggtcagcg cgtacagctt caaatgcgta tggaatttca gctcgcggaa
     2461 gatctccacc gtctcatgga tcggtccgcc cagcatttcc tgccaccgcc cgtagtacgc
     2521 gcggatcg
//
LOCUS       DATGKE010000509         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_526565, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000509 DATGKE010000000
VERSION     DATGKE010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526565"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..589)
                     /locus_tag="VKU83_08760"
     CDS             complement(83..589)
                     /locus_tag="VKU83_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93686.1"
                     /translation="MELITDQTIDLVNDRIQHMDEGQIGKVWDSFAREQPALVGYVLG
                     ESRELEMPDAREDVAYVLTIILLSFRELCPGIPSVTEAEFEKAFNEEFDEMESVFQDG
                     FDESDAMAVIESFCQPDLLQFAAATIYTEDGDEEESSNFTEEEAGLFIVIFKTAIGLL
                     HKKAGSIV"
     gene            650..2110
                     /locus_tag="VKU83_08765"
     CDS             650..2110
                     /locus_tag="VKU83_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HLI93687.1"
                     /translation="MSKNYSYKPKFTVSVSSEVGRLRRVLVHSPDSGLGKVVPSKAQD
                     WLFEDIVHLDTIRRKEYDFYTKILLYFLDPDKIRGRLAEIDAEAARRAFYKPGSDQFF
                     RSDKVVELQWLLADILADHAIKLKLVASVCAIETCSYEIQQDLQELGPVQLAKTFITG
                     ATDDDRLLFAPIPNFIFTRDIGIVINDYVLLNKPAKKARTREALLAKYIFYNHPLFAE
                     YQGKIIELTDTHHHFLLPREGDDRKVTLEGGDVMVVSKDHLLIGVSERTSTEAAHQAI
                     RVLFEKNVVKKVTIIRIPKKRDFMHIDTIFTQVKRNVWILLGTFSNKAIKHEHEDEIQ
                     RLLDNNKKEEKLRIIQFRKKDAGNPEYFDSLEDLLTDISKNDLQCTEKIRFIYSGNNE
                     FPFDAREQWTDSCNLLALKEGVVLGYDRNDKTVDAFRSNGFSIVEGAELIERLEKGEI
                     EPEQLENTLILMPSAELSRARGGFHCMSLPLHRDEL"
BASE COUNT          539 a          613 c          544 g          478 t
ORIGIN      
        1 cccaaaaata atagaaagtt gtttagcaaa ctaacagttg gtagtttttt ttgtaggcaa
       61 ctagatatga attggagccc catcaaacaa tgctcccggc cttcttatgc agcaacccta
      121 tggcagtctt gaagataaca atgaaaagcc cggcttcctc ttcggtaaaa ttgctgctct
      181 cctcctcgtc gccgtcctcg gtgtagatag tggccgcggc aaattgcagg agatcgggct
      241 ggcagaacga ttctatcaca gccatcgcat cgctctcatc gaaaccgtcc tggaaaacgg
      301 attccatctc gtcgaactcc tcattgaagg ccttttcgaa ctccgcctcc gtgaccgagg
      361 gaatgccggg acaaagctcc cggaagctca gcaggattat cgtaagcaca tacgccacat
      421 cttcccgggc gtcgggcatc tccagctccc tgctctcccc caggacatat cccaccaacg
      481 ccggctgctc gcgggcgaaa gaatcccata cctttccgat ctgaccctca tccatatgtt
      541 ggatccggtc attgacaagg tcgatcgtct ggtcggtaat aagctccatc ccttaatttt
      601 tgggtaaatt acctaatttg ccggattgct ggatcgaaaa caagattcta tgtctaaaaa
      661 ttattcctac aagcccaaat ttaccgtttc cgtgtcatcc gaggtaggcc gtcttcgccg
      721 cgtcctggtc cacagtccgg acagcgggct ggggaaggtc gtaccgtcca aggcccagga
      781 ctggcttttc gaggacatcg tccacctgga cactatccgg cggaaggagt atgattttta
      841 taccaagata ttgctctact tcctggaccc cgataagatc agaggcaggc tcgcggagat
      901 cgacgccgag gccgcacggc gcgcttttta taaacccggc agcgatcagt tcttccgttc
      961 cgataaagtg gtcgagctgc aatggctgtt ggccgatatc ctggcggacc acgctatcaa
     1021 gctgaagctg gtggcttcgg tatgcgccat cgaaacctgc tcgtacgaga ttcagcagga
     1081 cctgcaggaa ctgggcccgg tgcagctcgc caagaccttt atcaccggag ccaccgacga
     1141 tgaccgcctc ctcttcgccc cgatccccaa ttttattttt acccgggata tcggcatcgt
     1201 tatcaacgac tacgttctgc tcaacaaacc ggccaagaaa gcgcggacac gggaagccct
     1261 gttggccaaa tacattttct acaaccatcc gctgttcgcc gaataccagg gaaagatcat
     1321 cgagctcacg gatacccatc atcactttct tctgccgcgg gaaggggacg accgcaaggt
     1381 caccctggaa ggcggcgacg tcatggtggt aagcaaggat cacctgctca tcggcgtaag
     1441 tgagcgcact tcgacggagg ccgcccacca ggccatccgg gtacttttcg aaaagaatgt
     1501 ggtcaaaaag gtcaccatca tccgcatccc caagaaacgg gatttcatgc atatcgatac
     1561 tatctttacg caggtcaaac gcaatgtgtg gatcctgttg ggcacttttt ccaataaggc
     1621 gataaagcat gagcacgagg acgagatcca gcggctgctg gacaacaata agaaggaaga
     1681 aaagctgcgg atcatccagt tccgaaaaaa agacgcgggt aatcctgaat atttcgatag
     1741 cctggaagac ctgctcaccg atatcagcaa gaacgacctg caatgtaccg agaagatcag
     1801 attcatctac tccgggaata acgaattccc gttcgacgcc cgggaacagt ggaccgattc
     1861 ttgcaacctg ctggcgctca aagaaggcgt cgtgctgggt tacgatcgca acgataagac
     1921 cgtggatgcc ttccggtcca atggcttcag catcgtggag ggggcggagc tgatcgaaag
     1981 gctggaaaag ggcgagatcg agcccgaaca gctggagaat acgctgattc tgatgccttc
     2041 ggccgaactt tccagggccc ggggcggttt tcattgcatg agcctgccgc ttcaccgcga
     2101 cgagctataa aaactgacca ttatgcaaac cacgtctcat ctgctcatga tccgcccggt
     2161 caacttcagc ttca
//
LOCUS       DATGKE010000510         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1077623, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000510 DATGKE010000000
VERSION     DATGKE010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077623"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VKU83_08770"
     CDS             <1..1605
                     /locus_tag="VKU83_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLI93688.1"
                     /translation="PAATVQFRKDTIDFGSLQAKDRFGHSADLVRGRLFHHAFNDLGF
                     DFELNTNRLLLLDTKATDNNQFFGTVVGKAHITLTGPENNMRMDIKGAPTDSSNIYLP
                     PSVTRESGEADFLQWKVYGTELKKQTPDRKSSNLLVTLNVDANNYANVYMILDPLSGD
                     IIKTNGRGNLSMAIGTNQDLTLNGRYDIDHGSYNFTFQSFIHKPFVLKEGVGNYIQWN
                     GNPYDATIGIQAVYTAQNVQFSDLGISAASGLVLKNVASYRGDILVIATLKNRLMSPE
                     ITFQIELPDNSPIKNDQDALSLIQRIQSDPNELNKQVSCLVVLNSFVPLSTAPTAFDP
                     GQAAANVVVNSISGIISSAFRKQVSSVLQRVFKDNSIQVNFNPTVYNGTNVLGPETDP
                     TRLAYDRTNLNFSVAKSMFNEKLTVTVGSALDFGMTTQQEQQASFAFLPDITAAYKIT
                     PDGRVSLTFFYRDSYNYLSSGSHTLNSSGSSISYRRDFDRLDELFKKKKKKKAPAITA
                     PKEAVAPKEENTASNVDNPKPAPPDN"
     gene            complement(1607..2818)
                     /locus_tag="VKU83_08775"
     CDS             complement(1607..2818)
                     /locus_tag="VKU83_08775"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HLI93689.1"
                     /translation="MLSTIESAIEDIKRGKLVIVVDDEDRENEGDFVAAARKVSPETI
                     NFMSKHGRGLICAPLVEERCDELGLGLMVNNNTALHGTPFTVSVDLLGHGCGTGISAH
                     DRARTVQALIDPATKPEDLGRPGHIFPLRAKRGGVLRRAGHTEATIDLARLAGFEPAG
                     VLVEIMNEDGTMARSPQLTEIAAKFDLKIISIKDLIEFRLKTDSLIEEIVRVDMPTKY
                     GHFKLIAFKEKNTSNEHLALIKGEWKKDEPVLVRVHSSCFTGDILGSFRCDCGEQLHQ
                     AMRMVESEGKGVILYMNQEGRGIGLVNKLRAYKLQEDGLDTVEANLHLGFPMDKRDYG
                     VGAQILRSLGISKLRLISNNPQKRAGLLGYGLEIVESVPIEIRPNPHNERYLETKRDK
                     LGHEILKKVGG"
     gene            3246..>4265
                     /locus_tag="VKU83_08780"
     CDS             3246..>4265
                     /locus_tag="VKU83_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HLI93690.1"
                     /translation="MIKRLLLTAITCVSMAFAFAQETTSQIQGTVVTDSKAPLGGATV
                     VALHNPTGTKYTTTTRKDGHYYLPGLRVGGPYTLTVTYVGFKAVHQENITLVLGQDFY
                     GDFTMIPEATELQATVVSAARQNKIFNNAHTGTQEIVSRAQIEQLPTISRSIQDFTRL
                     EPSANGLSFAGTNPGENNVTVDGADFNNSFGLASTLGGQANAQPIALDAIDQIQVNIS
                     PYDVRQGGFTGAGINSVTRQGTNKFRGTIYDYIKGPGTQGYKVEDAVVAKSPFTYNVL
                     GGSLGGAFLPGRLFFFVNAEEDLQSAPATSYVASDANHPVTPGSVSQVSADSLSMLKQ
                     YLVSQY"
BASE COUNT          990 a         1349 c         1009 g          917 t
ORIGIN      
        1 cccgcggcca ccgtccagtt caggaaggac accatcgact ttgggtccct gcaggcaaag
       61 gaccgcttcg gccattccgc ggatcttgtc agggggcggc tcttccatca cgctttcaac
      121 gaccttggct ttgacttcga gctcaatacc aaccggctcc tcctgctcga caccaaagcc
      181 accgataata accaattctt cggcacggtc gtcggcaaag cacatatcac cctcaccggc
      241 cctgaaaaca atatgagaat ggatatcaag ggcgccccca ccgatagctc gaatatctac
      301 ctgccgcctt cggttacccg ggaaagcggc gaagccgatt tcctccaatg gaaggtatat
      361 gggaccgagc ttaagaaaca gacgcccgac aggaagagca gtaaccttct agtaacgctg
      421 aacgtggatg ccaacaatta tgcgaatgtc tatatgatcc ttgaccccct cagcggcgat
      481 atcataaaga cgaacgggcg cggcaacctt agcatggcca tcggcaccaa ccaggacctt
      541 accctcaatg gccgctacga catcgaccat ggaagctata acttcacgtt ccagtctttc
      601 atccacaaac cctttgtcct gaaggaaggc gtgggcaatt atatccaatg gaacggcaat
      661 ccttacgatg ctacgatcgg aatccaggcg gtctataccg cgcagaatgt ccagttcagt
      721 gatctgggca tctcggccgc aagcggactt gttctcaaga acgtagcgtc ttaccgcggc
      781 gatatccttg ttatcgcgac cctcaagaac aggctaatgt ccccggaaat caccttccag
      841 atcgagctgc cggataacag tccgatcaag aacgaccagg acgccctttc cctgatccaa
      901 aggatccaga gcgatccgaa tgaactgaac aaacaggtct catgcctggt cgtgctaaac
      961 agctttgtgc ccctgtcgac cgcccccaca gccttcgacc ctgggcaggc cgccgccaac
     1021 gtcgtcgtca acagtatttc cggtatcatc tccagcgcgt tccgaaaaca ggtctcatcg
     1081 gttctccaac gggtcttcaa agacaacagt atccaggtca atttcaaccc gacggtttat
     1141 aatggcacga atgtcctcgg ccccgaaacc gacccaaccc ggctcgccta cgaccgcacg
     1201 aacctgaatt tttccgtggc aaaaagcatg ttcaatgaaa agctgacggt aacggtcggc
     1261 agtgcgctcg actttgggat gaccacccag caggagcagc aagcctcatt cgcattcctg
     1321 cccgatatca ccgcggcata taagatcacg cccgacggcc gcgtgtccct cactttcttt
     1381 taccgcgact cttataatta cctctcgagc ggcagccata ccctcaacag ctcggggagc
     1441 agtatctcct accggcgcga cttcgaccgc ttagacgaat tgtttaagaa gaaaaagaag
     1501 aaaaaggcgc cggcgatcac agcccccaag gaggcggtcg cgccaaaaga agaaaatacc
     1561 gccagcaacg tcgacaaccc gaaacccgcc ccgccggaca attagcctac cccccgactt
     1621 tctttagtat ctcgtgcccc agcttatcac gcttggtttc cagataccgt tcattatgag
     1681 ggtttgggcg gatctctatc ggcaccgact caacgatctc caggccatag cccagcagcc
     1741 cggccctctt ttgcggattg ttgcttatca ggcgcagctt gctgatgccg agggaccgca
     1801 agatctgcgc gccaactccg tagtcccgct tatccatcgg aaaaccgaga tgcaggtttg
     1861 cttccactgt gtccagaccg tcttcctgaa gtttgtaagc ccgcagtttg ttgaccagcc
     1921 cgataccccg gccttcctgg ttcatgtata aaataactcc cttgccctca ctttccacca
     1981 tccgcattgc ctgatgcagc tgctccccgc aatcacatcg aaaggagccg aggatgtcgc
     2041 ccgtgaaaca cgaggaatgc acccgcacca gcacgggctc atctttcttc cactcgccct
     2101 tgatcagcgc caggtgttca ttactggtgt ttttttcctt gaaagcaatc agcttgaaat
     2161 gcccgtactt cgtcggcata tccactcgca cgatctcttc gatcagcgaa tcggtcttca
     2221 atctgaattc gataaggtcc ttgatcgaaa tgatcttcag gtcgaacttg gcggcgatct
     2281 ccgtcagctg cggcgaccgc gccatcgtgc cgtcctcgtt catgatctcc accaataccc
     2341 ccgccggctc aaagcccgcg agacgcgcca gatcaattgt cgcttcggta tgtcccgccc
     2401 gccttaacac cccaccgcgc ttggcccgca gcggaaaaat atgcccgggc ctgccgaggt
     2461 cctccggctt cgttgccgga tcgatcaggg cctggaccgt ccgggcgcgg tcatgagccg
     2521 aaatcccggt tccgcagcca tggcccagca gatcgacaga caccgtgaac ggcgtcccgt
     2581 gaagcgccgt attattattg accatcaatc ccaatcccag ttcgtcacac cgctcttcca
     2641 cgagcggagc gcagatcaga ccgcgtccat gcttactcat gaaattgatc gtctccgggg
     2701 aaactttcct ggcggccgcg acaaaatcgc cctcattttc ccgatcctca tcgtccacca
     2761 caataaccag tttgcctcgt ttgatatcct ctatggcgct ttcaatagtc gatagcatgc
     2821 ttatacagat tttgagttaa tgtcccggca tccgggccgg aatttccttt cggtgcaccc
     2881 gccagcacaa aattaacact tataacgcct taaagttaat aaaggatgcc tgctgagcct
     2941 ggccggtcta cccagaaacc acagcgcggc ccgcgggtta cctgtcccat gattgtcccg
     3001 cggcccatca gaccgcggtt tttcaggccg ggcgaccgac ctttcggacc ttttcccatg
     3061 tggaaaaacc ttccggtacg gtgtgaagct tctttttgtc atttttatat taatgatatt
     3121 tttaccgccg attgctgcct gttaataatt tcataaagag ccaggacccc ttcaaaatcg
     3181 gatagttagt ttctttgtgg ccagcgagat caaaactgag cacaaactgc ttaaaaaccc
     3241 tcattatgat aaaacgactg ctcttaacag caatcacttg tgtctccatg gccttcgcat
     3301 ttgcccagga gacgacttcc cagatccagg ggacggtggt cacggattcc aaggcgccgc
     3361 tcggaggcgc taccgtggtt gccctgcata acccgacggg gacaaagtac acaacaacta
     3421 cccgtaagga tggccattat taccttcctg gcctccgggt cggcggtccc tacaccctca
     3481 ccgtaaccta cgtcgggttc aaggcggtgc accaggagaa cataaccctt gtcctgggcc
     3541 aggacttcta cggggacttc accatgatcc cggaggcaac ggagctccag gcaacagtag
     3601 tttcagccgc ccgtcagaac aagatcttca acaacgcaca caccggtacc caggagatcg
     3661 tttccagggc tcagatcgaa cagctgccaa cgatctcacg ctccattcag gatttcaccc
     3721 ggcttgaacc gagcgccaac ggcctcagct tcgccggcac gaaccccggc gagaacaacg
     3781 tcacggttga cggcgccgat ttcaataatt ccttcggcct cgccagtacc ctcggcggtc
     3841 aggctaacgc acagcctata gccctcgatg ccatcgatca gatccaggtc aatatctcgc
     3901 cttacgacgt caggcagggc ggtttcacgg gcgccggcat caattccgtt acccgccagg
     3961 gaaccaacaa gttccggggt acaatatatg actacattaa ggggcccggc actcagggtt
     4021 acaaggtcga agatgccgtt gttgccaaaa gcccgttcac gtataatgtt ctcgggggca
     4081 gtctcggcgg cgcgttcctc ccgggcagac tgtttttctt cgtgaatgcc gaggaggacc
     4141 tccaaagcgc tccggccact tcttatgtcg catccgacgc gaaccatccg gtcacacctg
     4201 gcagcgtctc acaggtaagc gccgactcgc tgagcatgct gaaacagtac ctcgtgtcgc
     4261 agtac
//
LOCUS       DATGKE010000511         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1114352, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000511 DATGKE010000000
VERSION     DATGKE010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114352"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1449)
                     /locus_tag="VKU83_08785"
     CDS             complement(148..1449)
                     /locus_tag="VKU83_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HLI93691.1"
                     /translation="MKLKYTPASDAVKVIESGMRVFIHGSAATPVALIRAMQARHREI
                     HEVELVSITTMGDCDFDNPEWRDSFFVNSLFVSAETRQIANSMHGDYVPIFLSQIPQL
                     FRRGLFPLDVALIQVSPPDTHGYCSLGTSVDIARAAVDAARHVIAQVNPRMPRTHGDG
                     FVHIRDIDSMVWLESELPELKSAGGDDHIVSKIGHHVASLIEDGATLQLGIGNIPDQV
                     LKNLTDRHNLGLHTEMLSDGVIPLIESGVINNSQKRMNRGLSVTSFLNGTRKLFDFVD
                     DNPEVRVMDIGYVNDTAVIRQNPRVVAINSAIEVDLTGQVCADSLGTYQYSGIGGQMD
                     FIHGASLSDGGVPIIAMPSVTSTGISRIVPFLHEGAGVVTSRGHVHWVVTEYGRVFLF
                     GKNMKQRAQALISIAHPTHREWLEKAFYKRYRPTQVPAGMH"
     gene            complement(1486..2103)
                     /locus_tag="VKU83_08790"
     CDS             complement(1486..2103)
                     /locus_tag="VKU83_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93692.1"
                     /translation="MESVVPATGAGSCSTNTLRYAADLSSELGAAIRLDGDKIELFWR
                     EEEPFLIVTGATGGSAAGPFPCPVLSIPPHAVYGGVKKIVVACDRGDILAGLSDHTEF
                     LRKLHLLLGASFEFVHIVKGSQTNAGQIIQEYRSWKSRPDFFPQSPFFIQRESPQDGI
                     TDYLNTFPCDWLMVFPKDHSWIEFHRSQAESIAKLSNIPILRVYE"
     gene            2293..>2493
                     /locus_tag="VKU83_08795"
     CDS             2293..>2493
                     /locus_tag="VKU83_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93693.1"
                     /translation="MQQVFLPIDEGCFSTRILDWIDYLRTQGLLRLTVGLSSALGEKE
                     SLCRSIVLKLERFCRERDIRLAI"
BASE COUNT          551 a          659 c          721 g          562 t
ORIGIN      
        1 gtcagtcctc gagccatgct tatgaaaaac aggaaattcg agcctcaaaa aggggtatcg
       61 ccgcgtaaat tcccgagcaa tcgccgctgc ctgggggccg tgattagatg ggtggatcat
      121 atgatttcca tgttgttcat ccgcttctca atgcatgcct gcaggcactt gcgtaggcct
      181 gtaccgcttg taaaacgctt tttccagcca ttcgcgatgg gtgggatgcg caatgctgat
      241 cagcgcttgc gcccgctgct tcatattctt cccgaacagg aacaccctgc cgtattcggt
      301 gacgacccag tggacatggc cccgggacgt taccacgccg gctccctcgt gcaggaacgg
      361 aacaatgcgg gagattccag tggaggtgac cgagggcatg gcgatgatcg ggacgccgcc
      421 gtcagagagc gaggcgccat gaataaaatc catttgcccg ccgattcccg agtattgata
      481 ggttccaagg gaatccgcgc atacctgccc ggtcaggtcg acctcgatgg cgctgttaat
      541 ggcgacaacc cttgggtttt gcctgatgac ggcggtgtcg ttaacgtacc cgatatccat
      601 gacccggacc tccggattgt cgtcaacaaa gtcgaataat tttcgggtcc cattcagaaa
      661 ggaggtgacc gacaatcccc ggttcatcct tttctgactg ttattgatga ctcccgactc
      721 gatcagcggg atcacgccgt cggacagcat ctcggtatgc aagcctaaat tatgccggtc
      781 tgtgagattt ttgagcacct ggtcgggaat attaccgatc cccagttgaa gggtggcgcc
      841 gtcttcaatc agggaagcga catgatgacc aatcttgctg acgatgtggt cgtcgccgcc
      901 ggcggatttc agctcgggaa gttccgactc gagccagacc atcgaatcga tgtcccttat
      961 atggacgaac ccgtcaccgt gcgttcgcgg cattcgcggg ttgacctgag caatcacgtg
     1021 ccgcgcggca tcgacggcgg cgcgtgcgat gtctaccgag gtacccaatg aacaatagcc
     1081 gtgggtatcg gggggcgata cctggatcaa cgcgacgtcc aggggaaaca gacctcgccg
     1141 gaagagttgt gggatctggc tcagaaagat aggcacgtag tcgccgtgca tgctattggc
     1201 tatctggcgg gtctctgctg agacgaacag tgagttgaca aaaaaactgt cccgccattc
     1261 cggattgtcg aagtcacagt cgcccatcgt ggtaatactg accagttcga cctcgtggat
     1321 ctcccggtgc cgcgcctgca tggccctgat gagggcaacc ggcgtagcgg cgcttccgtg
     1381 gatgaaaacc cgcatgccgc tttcaatgac cttaacggcg tcacttgccg gagtatattt
     1441 caatttcatg tttttgatct taacgttgga tggttgttac agaacttatt catagacgcg
     1501 aaggatggga atgttgctga gttttgcgat gctttcggcc tggctccgat gaaattcaat
     1561 ccatgagtga tccttaggga acaccatcaa ccagtcgcag gggaaggtgt tcaaatagtc
     1621 ggtgatgccg tcctgcgggc tttcccgctg aatgaaaaac gggctttggg ggaagaaatc
     1681 cggacggctt ttccaggacc tgtattcctg gatgatctgc ccagcattgg tttgggaacc
     1741 tttaacgata tgcacaaatt caaagcttgc cccgagcagc agatggagct ttcggagaaa
     1801 ctccgtatga tcggacaagc cggcaaggat gtccccgcgg tcgcacgcaa ccacaatttt
     1861 tttgacgccg ccataaaccg cgtgaggcgg aatactcagg accgggcatg gaaacgggcc
     1921 ggcggcggag ccgccggtgg cgccggttac gatcaggaag ggttcttctt cgcgccagaa
     1981 caattcaatc ttatcgccgt ccagccggat ggcggcgccc agctcagaag aaagatccgc
     2041 ggcatacctc agggtattcg ttgagcaact gcctgcgcct gtggcgggga cgactgactc
     2101 cataattgac atttttgtaa aagtaatggc aaggccggga cgccaagctg accggcgtca
     2161 atcccgccat tgaatgaagt cagcccggga cctccggaaa gggccggaca ggcacattga
     2221 atcatggcgg gggctgacgt acgtcaggtg ccagtgggct accgggcgat atttttaggg
     2281 aaattctgga caatgcaaca agtttttctt ccgatcgatg aaggatgttt ttcaacccga
     2341 atcctggatt ggatcgacta tttgcgcacg cagggcctcc tgcgcctgac cgtcggatta
     2401 tcttccgcgt tgggtgaaaa ggaaagcctt tgccgcagca tcgtcctgaa actggaacgt
     2461 ttttgccggg aacgcgatat ccggttggcg att
//
LOCUS       DATGKE010000512         5215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1352715, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000512 DATGKE010000000
VERSION     DATGKE010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352715"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VKU83_08800"
     CDS             <1..896
                     /locus_tag="VKU83_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93694.1"
                     /translation="VYQFNTDRALADEAAVTFNTGLVHGPKTARPLTQGFDGWTAEDS
                     AYLRRNKYIQREQVHADAMENIKAKTLAPAFVTARPKSAADKLDERYTSGMFSGGDAY
                     SFDIMDDPTALGMTDIFQYLQGRVPGLLVNPGQGPGGVPSLSWRGGRPTLYLNEMQVD
                     PSQLQSVSVPDVAYLKVFRPGSGVGFGGGSGGTIAVYTKKGGDSKNTDPNFKGLPKTT
                     LIGYSMPKEFYSPNYLENPQVEKEDLRTTLYWKPYLMTDKDNSRVSVDFFNNDVTKKL
                     RVVLEGFNENGKLTHIEQIIQ"
     gene            900..2021
                     /locus_tag="VKU83_08805"
     CDS             900..2021
                     /locus_tag="VKU83_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HLI93695.1"
                     /translation="MQISEVTGHLEQLAPPLYQEHYDNAGLLTGSGNWDCKGVLVALD
                     ATEEVILEAAARGCNLVVSHHPIIFHGLKKISSSNYVGRTIIAAIKNDIAVYAIHTNL
                     DNVMSAGVSGRIADKLELEERRVLSPRPGSLQKLFCFVPVDHLEAVRSAVFAAGAGHI
                     GAYSECSYQAEGFGTFKGEAGSQPYVGTVGQRHEEREARLEVILPVHLSRAVVDAMIA
                     AHPYEEVAYDLVPLANVHAGIGAGLVGELPAEVGEQAFLDRLCRIFGVPAVRHTRLTG
                     RPVKKVAVCGGAGSLFIPNALSAGANFYVTSDVKYHEFFDANGQLVIADIGHFESEQF
                     TADLLVGVLREKFSNFAVLKSDIRTNPVHYHISAGGRNP"
     gene            2030..2785
                     /locus_tag="VKU83_08810"
     CDS             2030..2785
                     /locus_tag="VKU83_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HLI93696.1"
                     /translation="MSNVKEFSVEEKLTSLVTLQKIESKLDEYKILKGELPMEVSDLE
                     DEITGLHSRQTRVEEEINGIQEFINQKKEAIKEADALIKKYDKQSQNVKNSREFEAIN
                     KEIEMQQLEVKLAEKHIKDANEEIAEKVQLLEKAKKNIGTKESVLKNKKDELDKIIAT
                     TEKEEKHFVKLSAEAREKVEPRILHSYDRIRSNYRNGLAVVPVLRDACGGCFNAIPPQ
                     RQSEIRQRKKIIVCENCGRILVDNELNDSVEVK"
     gene            2795..4273
                     /locus_tag="VKU83_08815"
     CDS             2795..4273
                     /locus_tag="VKU83_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93697.1"
                     /translation="MRWVLLSFLFFTVSGYGQPHFDFNPDCRKAYEAIVQLRLDEGSR
                     LLEAEKKNDADNLIPVLLDNYIDFFKLFFNEDPALYQAAKGRLDQRLARLSAGPESSP
                     FYLFARSIIHFQWAAIRIKFGNNLSAGLDFRRSFLESQECREKFPSFGPATMLSGAMK
                     VVAGTIPDGYKWLSNLLGIRGSVREGMADLENFLRLGDEWSVLYRDEATFYYLYLRFY
                     ILNQHRQVFEYIRQHQLDVRNNYLFAYVDANLSINDQRSEAARMVIQGRNKDAGYLEM
                     PVWDLEMGYACLNHLDAEAPVYLERFLARFRGRFYVKDALQKLSWYYYLAGDQRLADS
                     LRRRVLRQGSAESDADRQAVKEARSGVWPDKTLLRARLLTDGGYYEDALRSLQGVGTS
                     AYTRPEDRCELSYRLGRIYDGLGRKDEAITAYLTTIKTGEHLKEYYAARAALQAGEIY
                     ESRGDLERAMGFYQKCLSMKDHDFKNSLDQRAKAGIARCEGE"
     gene            4428..>5215
                     /locus_tag="VKU83_08820"
     CDS             4428..>5215
                     /locus_tag="VKU83_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI93698.1"
                     /translation="MIRRIQIRNYAIIDELSIDFSPGMTVITGETGAGKSILMGALSL
                     ILGDRADSSVLLQQDKKCIVEGVFAVGGRADVRAFFIGNDLEPEEGDVIIRREIAPSG
                     KSRAFVNDTPVNLEQLRRLSSLLVDLHRQFNTLELGEMDFQREVLDALAGQTAAVSEY
                     RSAYRNWQAAGRELAALQARQAQFNAALDYNKFLAEELREAGFREQELEDLDQELKLL
                     SHSEGIKTALSKAYYDLKEGERPLLQELRSLQHSLQAFSTYHQG"
BASE COUNT         1231 a         1248 c         1631 g         1105 t
ORIGIN      
        1 cggtttatca gttcaatacc gaccgggcgc tcgccgacga ggcggcagtg acgttcaata
       61 cgggcctcgt tcacgggccg aaaaccgcca ggccgttgac ccagggattt gatggttgga
      121 cggcggaaga ttcggcttac ctgcggcgga acaagtatat ccagcgggag caggtgcatg
      181 ccgatgccat ggaaaatatc aaggcgaaga ccctggcgcc cgcgttcgtc accgcgaggc
      241 cgaaatctgc cgccgacaag ctggacgagc gctatacctc cggaatgttc agcggcggcg
      301 acgcttattc atttgatatc atggacgacc cgacggcctt gggaatgacg gatatctttc
      361 aatatttgca gggcagggta cccgggctgc tcgttaaccc cggccagggg cccggcgggg
      421 taccatcgtt gagttggcgc gggggccgcc caacccttta tctgaacgaa atgcaggttg
      481 atccttcgca gcttcaaagt gtctccgtac ccgacgtagc atacctcaaa gtcttccggc
      541 cggggtccgg ggtaggattc ggcggcgggt ctggcggaac gatcgccgtg tacaccaaaa
      601 agggcggcga cagcaagaac accgacccga actttaaagg attgccgaag acaacgctta
      661 tcggttactc aatgcccaag gaattctatt cgccgaatta cctggagaac ccgcaggtag
      721 aaaaagaaga cctgcggaca accttgtact ggaaacctta cctgatgacc gacaaggaca
      781 acagccgggt aagcgtcgac ttctttaaca acgatgtcac gaagaagctg agggtggtgc
      841 tggagggttt caatgagaac ggcaaactga cgcatatcga gcagatcatt caataaatca
      901 tgcagatctc agaagttacc ggtcacctgg agcagttggc tccgcccctt taccaggagc
      961 attatgacaa tgcggggctg ctaacgggca gcggaaactg ggactgtaaa ggggtgcttg
     1021 tggcgctgga tgccaccgaa gaggtgatcc tcgaggccgc ggcgagggga tgcaacctgg
     1081 ttgtgagtca tcacccgatc attttccacg gcttgaaaaa gatcagcagc tcgaattacg
     1141 tcggccgcac gatcattgcg gcaatcaaaa acgatatcgc ggtgtatgcg atccatacga
     1201 atctcgacaa cgttatgtca gccggagtaa gcgggcggat tgccgacaaa ctggagctgg
     1261 aagagcgacg ggtgttgtca cccaggccgg gaagcctgca gaagctattt tgcttcgtgc
     1321 cggtcgacca tctcgaggcg gtgcgttcgg cggtttttgc tgccggcgcc ggccatatcg
     1381 gtgcgtacag tgaatgcagt taccaggccg agggcttcgg taccttcaaa ggcgaagccg
     1441 gatcgcagcc ctacgtgggg acggtcggac agcggcatga ggaaagagag gccaggctcg
     1501 aggtgatatt gccggtgcat ctcagccgcg cagtagtcga cgccatgatt gccgcgcatc
     1561 cctatgagga ggtagcctat gacctcgtcc cgctcgccaa cgtgcacgca gggatcgggg
     1621 ccgggctggt gggcgagctg ccagcggagg ttggtgagca ggcattcctg gaccggcttt
     1681 gccgcatatt tggcgtcccg gcggtgcggc atacccggct aaccgggcga cccgtgaaaa
     1741 aggtagctgt atgcggcggt gcaggtagtc tattcattcc caatgcctta tcggccgggg
     1801 caaactttta tgtaacgtcg gacgtcaaat atcatgagtt ttttgacgct aatggccagc
     1861 ttgttatcgc ggatatcggg cactttgaaa gtgagcaatt cacggccgat ctgctggttg
     1921 gcgttttaag agaaaaattc agtaattttg cggtccttaa atcggatatc cggacgaacc
     1981 ccgttcatta tcatatttcg gccggtggcc ggaacccata atttaaacta tgtcaaacgt
     2041 aaaagaattc tctgtcgagg aaaagctcac ttctttggtg actctccaaa aaatcgaatc
     2101 caagctggac gagtacaaaa tcctgaaggg cgagcttccg atggaggtga gtgatctcga
     2161 ggatgagatc acgggattgc attccaggca gacgcgggta gaagaggaga tcaatggcat
     2221 ccaggaattc atcaatcaga agaaagaggc catcaaggaa gcggacgcgt tgattaagaa
     2281 atatgataag cagagccaga acgtgaagaa cagccgggag tttgaggcaa ttaacaagga
     2341 aatagagatg cagcagctgg aggtgaaact ggcagagaag catattaaag atgccaatga
     2401 ggagatcgcc gagaaggttc agttactcga gaaggccaag aaaaatatcg gaacgaagga
     2461 atcggtgctg aagaataaga aggacgagct ggataagatc atcgccacta cagagaagga
     2521 ggaaaaacat tttgtaaaat tgtccgcgga agcccgggaa aaggtggagc cgcggatctt
     2581 gcattcatat gaccgcatcc ggagcaatta ccgcaacggc ctggcggtcg tgccggtgtt
     2641 gcgggatgcc tgcggcggtt gtttcaatgc gatcccccct cagcgccaaa gcgaaatccg
     2701 tcaacgtaaa aagatcattg tctgcgaaaa ttgcggccgc attcttgtcg acaatgagct
     2761 gaacgactct gttgaagtca agtaagcgac cgtggtgcgg tgggtacttc tgagttttct
     2821 gttctttact gtttcgggtt acgggcagcc gcatttcgat ttcaatccgg attgccggaa
     2881 agcctacgag gcgatcgttc agttgcggct cgatgaggga agcaggctgc tggaagccga
     2941 aaaaaagaat gatgcggata acctcatccc ggtcctactg gacaattaca ttgatttttt
     3001 caaactattt ttcaatgagg atccggccct ttatcaggcg gcgaagggaa ggctggacca
     3061 acgtctcgcg cggctgagcg ccgggccgga gagttctcct ttttacctgt tcgccaggag
     3121 catcatccat tttcagtggg cggcgatccg catcaaattc ggcaataatc tcagtgcagg
     3181 tctcgatttc cgccgctcat tcctggagag ccaggaatgc agggaaaagt ttccttcgtt
     3241 cggcccggcc acgatgttga gcggggcgat gaaggtggtc gcgggtacga tcccggacgg
     3301 gtacaaatgg ctcagcaact tattgggcat ccggggaagc gtcagggaag gcatggcgga
     3361 tctcgaaaat ttcctgcgcc tgggagatga gtggtcggtg ttgtacaggg atgaggcgac
     3421 cttctactat ttgtatctca ggttctatat cctcaatcag catcgccagg tcttcgaata
     3481 catccggcag caccagctgg acgtccgcaa caactatctg tttgcctatg tggatgcgaa
     3541 cttatcgatc aacgatcaac gctccgaggc ggcccggatg gtgattcagg gccggaataa
     3601 ggatgcgggt tatctcgaga tgccggtttg ggatctggag atgggttatg cctgtctcaa
     3661 ccatctggat gctgaagcgc ccgtgtacct cgagcggttc ctggcccgtt tccgtgggcg
     3721 gttctatgtc aaggatgcgt tgcaaaagct tagctggtat tattatctgg caggagacca
     3781 gcgccttgcc gattcgctgc ggcgccgcgt cttgcgtcag ggttcggcgg agtcggatgc
     3841 cgaccggcaa gccgtaaagg aagcccggag cggcgtttgg ccggataaga ctttgttgcg
     3901 ggcgcgcctg ttgacggatg ggggatacta tgaggacgcg ctgcgttcct tgcagggggt
     3961 cggcacgtcc gcatacacgc gaccggaaga caggtgcgag ttgtcatacc ggctgggaag
     4021 gatctatgac gggctgggca ggaaggatga agcgattact gcttatctca ccaccatcaa
     4081 gactggcgag catttgaaag agtactatgc ggcgcgggcg gcgttgcagg caggggagat
     4141 ctatgagtcg cgcggggacc ttgagcgcgc aatggggttt tatcaaaaat gtttgtctat
     4201 gaaggaccac gacttcaaaa attcgctcga ccagcgggcg aaggcgggta ttgcaaggtg
     4261 cgaaggagag tagccctggc gcgtgggtag cttgcagggt gccggggagc tttcccggtt
     4321 aattgggccg atttcggccc aattaagaaa aaggcttcct gccgcggctg gcgccattgt
     4381 gggaattttg tcggttgcgt caaaaccgaa atacttaaat tggacgcatg attcgacgga
     4441 tccagattcg gaattatgcc ataatcgacg agctgagcat cgatttctcg ccggggatga
     4501 cggtgatcac tggggagacc ggcgcgggca agtcgatcct gatgggggct ttgtcgttga
     4561 tcctcggaga ccgggcggat agttcggttt tgttgcaaca ggataagaaa tgcatcgtgg
     4621 agggggtgtt tgcagtgggg ggcagggcgg acgtgcgcgc ttttttcatt gggaacgatc
     4681 tcgagccgga ggaaggcgac gtgatcatcc ggcgggagat tgcgccgagc ggaaagtcca
     4741 gggcgtttgt gaacgatacg ccggtcaacc tcgaacaatt gcgaaggctg agttctttgc
     4801 tcgtggacct tcaccggcaa tttaacaccc tcgagctggg ggagatggac tttcaacgcg
     4861 aggtgctgga cgcgttggcg gggcaaaccg cggcggtgag cgaataccgg tcggcttacc
     4921 ggaactggca ggctgccgga agggaattgg cggctttgca ggcaagacag gcgcaattta
     4981 atgcggcatt ggattacaat aaattcctcg cagaagaatt aagggaggcg ggcttcaggg
     5041 agcaggaact ggaggatctg gaccaggaac tgaaactgct cagtcactcc gaggggatca
     5101 agacggcatt gtcaaaggct tattatgacc tgaaagaggg ggagcggcca ttattgcagg
     5161 aactgcggtc cttgcagcat tcgcttcagg cattttcaac ctatcaccag ggatt
//
LOCUS       DATGKE010000513         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_296929, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000513 DATGKE010000000
VERSION     DATGKE010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296929"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1889)
                     /locus_tag="VKU83_08825"
     CDS             complement(180..1889)
                     /locus_tag="VKU83_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93699.1"
                     /translation="MTTLVRKILRVLTPGEKRRLFELTAMDMVSSVADIAGLAFLLWV
                     VRLYAQVSAGSGTGGMPSWLTDPHSLWPIGLLFLFFMIKNWLSFALYSAQARFRYGVA
                     SRISRQNLLYYLEGEYAQYAHTDSAAHVLKIAQQPIEFCQFVLGGLQLGTTEATLISV
                     TVVAVLLFNAKLFLLLLLLLLPPVIITAWLAKRKLHAARTFIKSSRDVMWQHLQESIA
                     GYVESSLYDRKDFFAGRYGDSQTILNRHLATVQSLQGAPSRLAELFAVFGLMALIGIG
                     HFAGNQHSAEFVTLGAFLAAAYKIIPGIARLLNLTGQMKTYEFTVNGLLERTARAGQS
                     AAGWVEYPEAIHSIIFNEVGFRYGDQQVLEAVDLVLRRGDFLGIQGDSGRGKTTLFNI
                     MLGFSAEATGKVMINGTPTRAADRRRYWDRIAYVKQQPFILHDSILVNITLLEDGYDE
                     QRLQYAVSLSGLDELIRKLPGGLLTTVTENGKNISGGQRQRIAFARALYKQADLILLD
                     EPFSELDEASEARMLRRLAELAASGKMVVMITHNRQSLTYCTHSISLDKPVPAGHPAA
                     GNS"
     gene            complement(1895..>2420)
                     /locus_tag="VKU83_08830"
     CDS             complement(1895..>2420)
                     /locus_tag="VKU83_08830"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HLI93700.1"
                     /translation="LLRQTEGSGMPVSVSILGTVTDFTQHITDEVFLNTQDRIAYSAE
                     LLIARKIGRNLSLQVSPAYLYYNFVPTPADKNGVFAVGLGGRMKITHRMSIDAEYDLV
                     PKNQIVSYPVYNSFSLGWDIETGGHVFQLVFTNSQSMVPTQFLTQTTGTWGKGDIYFG
                     FNISRNFNLKKRSK"
BASE COUNT          535 a          706 c          682 g          497 t
ORIGIN      
        1 ccagcagctt gatgaacacg atgcggccag gcggccgacc ggtcgaatgg tcccgccgca
       61 acagcaagcc aagcaccctg gcgagcggaa gcaaaaacgc gatcagcaaa tagccagcat
      121 accagtcgat ggtctgtttc ctgcggattt gcatatacgg taagaacggg cccacgcttt
      181 tacgagttgc ctgcggcagg atgtcccgcg gggaccggct tgtcgagtga aatgctgtgc
      241 gtgcagtacg tgaggctctg ccgattgtgg gtgatcatga ctaccatttt ccccgatgcc
      301 gccagctcgg ccaaccggcg cagcatacgc gcttccgacg cctcatccag ctcgctgaaa
      361 ggttcgtcca acaggataag atcggcctgc ttgtatagcg cccgcgcaaa ggcgatccgc
      421 tggcgctgcc cgccgctgat attttttccg ttctccgtta ccgtcgtcag aagcccgccg
      481 ggaagcttcc ggatcaattc atccaggccg gacaaggata ccgcatactg gagccgctgc
      541 tcatcgtagc catcttcgag gagcgtgatg ttaacgagaa tggaatcgtg caagataaaa
      601 ggctgttgtt tcacgtaggc gatccggtcc caatagcggc ggcggtctgc ggcgcgggtc
      661 ggcgtcccat tgatcatgac ctttcccgtt gcttcggccg agaaacccag cataatgttg
      721 aacagcgtgg tcttcccgcg gccggaatct ccctggatcc ccaggaaatc accccgtcgc
      781 agcacgaggt cgacggcttc taggacctgc tgatcaccat acctgaagcc tacttcgtta
      841 aagatgatcg aatggatcgc ttccggatat tccacccatc cggccgccga ctgccccgcc
      901 cgggctgtcc tttccaaaag accattcacg gtaaattcgt aggtcttcat ttggccagtc
      961 aggtttaata accgggcaat ccccggaatg atcttgtagg cggcggccag gaaggcgccc
     1021 agcgtaacga attccgcgct atgttgatta ccggcaaaat ggccgatgcc gatcagggcc
     1081 atcagcccga ataccgcgaa gagctccgcg agtcgcgaag gggcgccctg caaggattgc
     1141 acggtcgcca ggtgccggtt aaggattgtc tgggaatccc cgtagcggcc ggcaaagaaa
     1201 tccttccggt cgtacaggct gctttccacg tagccggcga tcgactcctg taagtgttgc
     1261 cacatgacat cccggctgct cttgataaag gtcctggccg catgcagttt tcttttcgcc
     1321 agccacgccg tgataatgac ggggggcagg agcagcaaca ggagcaggag gaatagtttg
     1381 gcattgaaaa gcaggacagc caccacggtt acggagatca gcgttgcttc cgtcgtcccc
     1441 agctggaggc cgcccagcac gaactggcag aattcgatcg gctgttgcgc gatcttaaga
     1501 acgtgcgctg ccgagtcggt atgcgcatac tgagcatact caccttcgag gtaatacagc
     1561 aggttttgcc gggaaatgcg cgatgccacg ccatatcgaa accgggcctg ggcggagtac
     1621 aaggcaaagc tcagccagtt cttgatcatg aaaaagagga agagcaagcc tatgggccaa
     1681 agcgaatgcg gatcggtcag ccaggacggc atcccgcccg tcccgcttcc ggccgaaacc
     1741 tgcgcataaa gccggacgac ccaaagcaga aaggcgaggc cagcgatatc cgccacgctg
     1801 ctgaccatgt ccatggccgt cagctcgaac aaccgccgct tttctcccgg ggtcagcacc
     1861 cgcaatatct tccggaccag tgtcgtcaca gcatctattt actgcgcttc tttaaattga
     1921 aattccttga aatattgaac ccaaaataaa tatccccttt gccccatgtt cccgttgttt
     1981 gggtcaggaa ctgggtcggt accatgcttt gggaattggt gaaaaccaat tggaagacgt
     2041 gtccgcccgt ctcgatatcc cagccgaggg aaaaggagtt gtacaccggg taggaaacga
     2101 tctgattctt cggcaccagg tcgtattccg catcgatact catccggtgg gtgatcttca
     2161 tccggccgcc caggcccact gcaaagaccc cgttcttgtc ggccggcgtg ggtacgaaat
     2221 tataatacag atatgccggg gagacctgga gcgacaggtt ccggccgatt ttcctggcga
     2281 tcagcaattc cgccgaataa gctatcctgt cctgggtgtt taggaacacc tcgtcggtga
     2341 tgtgctgcgt aaaatcggtg accgtgccca ggatacttac cgacacgggc atccctgatc
     2401 cctcggtctg gcggaggagc
//
LOCUS       DATGKE010000514         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1362539, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000514 DATGKE010000000
VERSION     DATGKE010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362539"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VKU83_08835"
     CDS             complement(<1..596)
                     /locus_tag="VKU83_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLI93701.1"
                     /translation="MDTQQQEQKQYLWWQDGIIYQVYPRSFQDSNADGIGDLQGIIRR
                     LPYLKRLGIKAIWISPIYPSPMADFGYDISDYEGIWPGFGTMADFDELLSRIHGLDMK
                     LILDLVPNHSSDEHPWFLESRSSRENPKRDWYIWKDPKPDGSPPNNWLSVFGGSVWEW
                     DEKTKQYYYHAFLVKQPDLNWRNPEVEKAMLDVMRFWL"
     gene            623..2329
                     /locus_tag="VKU83_08840"
     CDS             623..2329
                     /locus_tag="VKU83_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HLI93702.1"
                     /translation="MKKKWWKEAVIYQVYPRSFKDSDGDGVGDLPGILSEIDYISSLG
                     VDAVWINPVYASPGEDNGYDISDFRALDPGFGSMDDLGRLIEALHGRNLKIIMDMVVN
                     HTSDEHEWFRLARRSREDPHYGWYHWWPAEKGEPPHRCGFFDPEGKAWKYNEPTHSWY
                     LHYFSPHQPDLNWDNAGVRREIYSMLKFWLDKGVDGFRMDAISFISKDTSWPVISPEK
                     LDREFGGDWGNYYAAGPRLHEYLRELHSAVLRDRDAVIIGESSGIPAEKAADFVGEDR
                     EELDMIYQFESIQVGYMPGEFKKVAPEGYSLVRLKEVFTRWDRLLEGRGWNILYLGNH
                     DQPRMVSRWGDDGERFGSRSAKLLFTFLLTMRGTPSLYNGDELGMTNIRFTRIEEYRD
                     IETLRVYGQIREKGGDTDAFLRDQQLTARDNSRTPFQWTGGRNAGFTTGAPWMPVNEN
                     YRVINRELEERSEDSVLWYVRRLLQFRRHHPMLVYGGYEMLAPGDPHIYAYRRGDAAD
                     ALLVLLNFSGSEQAFPGNWAGPVRINNCPELMRDRNRIVLQPWQAVVIGGDAAQAVAP
                     GP"
     gene            complement(2326..>3513)
                     /locus_tag="VKU83_08845"
     CDS             complement(2326..>3513)
                     /locus_tag="VKU83_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLI93703.1"
                     /translation="KAIVRFMPFPHQSAGQKVLGAWKLKLKYGWGNFTTLETKKVINA
                     QLFIILSAAILYFARPVLMPLAMAGMVALVLMPLCRRLEQAGCHTVVAALICGLIFAL
                     LVAAIILFLVWHVQHIAADFSDIRQHFTGYLHGIRKYLHDRFGMDTLKKDSPLPIPVQ
                     PDSNGIGKIAASIMGTVLSVAINLILILVYMIMLLCMRHEVRVFILRLCNPGSTGKAE
                     VVIDQSAAVVKQYLRGLAIIIVCLWIMYSIGFTLVGVHTPIFFAILCGTLEIIPFVGN
                     ITGTTLTSLMALSQGGGFGMVLGVVCTYMLIQGIQFYIISPLVMQTQVSIHPLFTILI
                     LFVGDLVWGIPGMILAIPVLGIVKIVCDHIDYLQSFGSLLGKNRPVKGRSAWFRRRRA
                     GNP"
BASE COUNT          805 a          925 c         1063 g          720 t
ORIGIN      
        1 tcgagccaga agcgcatgac gtcgagcatg gccttttcca cttcgggatt gcgccagttg
       61 aggtccggtt gtttgacgag gaaggcatga taataatatt gttttgtctt ttcatcccat
      121 tcccacacgc tgccgccgaa aacgctcagc cagttgttgg gcgggctgcc atcgggcttc
      181 gggtccttcc agatatacca gtcgcgcttt ggattttcgc gggaactccg cgattcaagg
      241 aaccagggat gttcatccga actatgattg gggacgaggt cgaggatcag tttcatgtcc
      301 agtccgtgga tccggctgag gagctcgtca aaatccgcca tcgtcccgaa tcccggccag
      361 atgccttcat agtcggaaat gtcgtagcca aaatcagcca tcggcgatgg atagattggg
      421 gagatccaga tcgctttgat gcccaaacgt ttgaggtatg gcaagcggcg gatgatgccc
      481 tgcaggtcgc cgatcccgtc ggcattactg tcctggaagg accgtggata gacctggtag
      541 atgatgccat cctgccacca caggtattgt ttttgttctt gttgttgggt gtccatgatc
      601 caaaaactgc aatgtcagtg ccatgaaaaa gaaatggtgg aaagaagcgg tcatttatca
      661 ggtctaccca cgcagcttta aagatagcga cggcgatggg gtaggagatc tgccgggtat
      721 cctgtccgag atcgactata tctcgtcgtt gggggtagac gcggtctgga tcaatccggt
      781 atatgcatca ccgggagaag ataacgggta tgatatcagc gatttccgcg cgctcgatcc
      841 gggattcggc agcatggacg acctcgggcg cctgatcgag gcgttacacg ggcgcaacct
      901 gaagatcatc atggatatgg tggtcaacca taccagcgac gagcatgaat ggttccggct
      961 tgcacgccgc tcgagggagg acccgcatta cgggtggtat cactggtggc ccgccgagaa
     1021 aggcgagcct ccgcaccgct gcgggttctt cgatccggag ggaaaggcat ggaaatacaa
     1081 cgagccgacc cactcctggt acttgcatta tttctccccg caccagccgg acctcaactg
     1141 ggataacgcc ggggttcgcc gggaaatcta ctcgatgctg aagttctggc tggacaaggg
     1201 cgtcgatggt ttccggatgg atgcgatcag ttttatctcg aaggatacga gctggccggt
     1261 gatcagtccg gaaaagctgg accgggaatt cggcggcgac tgggggaatt attatgccgc
     1321 aggtccaagg cttcacgagt atttgcgcga acttcattcg gcggtgctcc gcgaccggga
     1381 cgcggtgatc atcggggaga gctccggcat tccggcggag aaagcggccg acttcgtcgg
     1441 cgaagatcgc gaagagctcg acatgatcta ccagttcgaa agcatccagg taggctacat
     1501 gccaggcgag ttcaagaagg tggcgccgga gggatactcg cttgtccggt taaaggaggt
     1561 atttacccgc tgggaccggt tgctggaggg gagaggatgg aatatcctgt acctcggcaa
     1621 tcatgatcag cccagaatgg tgtcccgctg gggagacgac ggtgagcggt tcggcagccg
     1681 ctcggcgaag ctgctgttca cgttcttatt gaccatgcgg ggcacgcctt ccctctacaa
     1741 tggcgatgaa ctgggaatga ccaatatccg gtttacgcgc attgaagagt accgggacat
     1801 cgaaacgcta cgggtctacg ggcagatccg cgaaaagggc ggcgacactg acgcgttcct
     1861 gcgcgaccag cagctaacgg cgagggataa cagcaggacg cccttccaat ggaccggcgg
     1921 ccggaatgcg ggttttacga ccggggcgcc atggatgcct gtaaatgaga attaccgggt
     1981 catcaaccgc gagttggagg aacgcagcga ggattccgtg ctctggtatg taaggcggct
     2041 gttacagttc cggcggcatc atcccatgct cgtatacggc ggttatgaaa tgcttgctcc
     2101 cggagacccg catatctatg cctatcgccg tggcgacgca gcggatgcgt tgcttgtcct
     2161 gctgaatttt tccggcagtg aacaggcctt tccgggaaac tgggcgggcc cggtgcgcat
     2221 caacaattgc cctgagctga tgagggaccg gaaccggatc gtgttgcagc cctggcaggc
     2281 cgtggttatc ggcggtgacg cggcacaggc ggtcgcaccg ggcccttagg ggttgccggc
     2341 ccggcggcgc cggaaccatg cagaccgccc cttgaccggc cggttctttc ccaaaaggct
     2401 gccaaacgac tgaaggtagt cgatatgatc gcaaacaatc ttaacgatcc caagcaccgg
     2461 tatcgcgagg atcattcccg ggatccccca taccaggtcg ccgacaaaga ggatgaggat
     2521 cgtgaagagc ggatggatgc tgacctgcgt ctgcatgacc aacggggaaa tgatatagaa
     2581 ctggatgccc tggatcagca tataagtaca taccacgccc agtaccatgc caaagccgcc
     2641 gccctgtgaa agggccatca gggaggtgag cgtagtcccc gttatattgc cgacaaacgg
     2701 gatgatctca agggtgccgc agaggatggc gaaaaaaatg ggcgtgtgga cgccgaccag
     2761 ggtaaaaccg atactgtaca tgatccagag gcagacaatg atgatggcca ggccgcgaag
     2821 atattgtttt accaccgccg ccgactgatc gatgaccact tcggcctttc ctgtcgagcc
     2881 cgggttgcag agccgcagaa tgaatacccg cacctcatgc cgcatgcaga gcagcataat
     2941 catgtatacg aggatcagga tgaggttgat cgctaccgaa aggacggtgc ccataatcga
     3001 ggccgctatt ttaccgatgc cgttgctgtc cggttgcacc gggatcggca gcgggctatc
     3061 cttcttcagc gtatccatcc cgaacctgtc gtggagatat ttcctgatcc catggagata
     3121 accggtaaaa tgctgccgga tatcggaaaa atccgccgca atgtgctgga catgccaaac
     3181 gaggaacagg atgatcgccg cgacgaggag cgcgaatatc agcccgcaga tgagggcggc
     3241 aacaaccgta tgacaacctg cttgctccag ccggcggcag aggggcatca ataccagcgc
     3301 aaccattccc gccatcgcca atggcatgag gacgggtctg gcaaaataga ggatggctgc
     3361 cgacaggatg atgaacaact gcgcgttaat aacttttttg gtctccaatg ttgtaaaatt
     3421 tccccaaccg tacttgagtt tcaacttcca tgccccaagg accttttggc ccgccgattg
     3481 atggggaaaa ggcatgaaac gcacgatagc ctt
//
LOCUS       DATGKE010000515         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1393273, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000515 DATGKE010000000
VERSION     DATGKE010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393273"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VKU83_08850"
     CDS             <1..269
                     /locus_tag="VKU83_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HLI93704.1"
                     /translation="PPVNSLKSMIGHSLSAAGSIESVAAVLQIVHGFIHPNINLEDPN
                     PEIMKQVGPDCLPVTMIEKELRTVAKANFGFGDVNACLIFSKYD"
     gene            271..519
                     /locus_tag="VKU83_08855"
     CDS             271..519
                     /locus_tag="VKU83_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93705.1"
                     /translation="MERREILDELKKVLYPYTENKELLENIHENTSLVKDLKINSANL
                     VDIIIDAEAKYNIEIDFDSAEKIVTVGDCVNAIEEKVN"
     gene            528..1202
                     /locus_tag="VKU83_08860"
     CDS             528..1202
                     /locus_tag="VKU83_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLI93706.1"
                     /translation="MSAGNDIVALAATDSDRTCRYRFFSRIITQHEKQQYFGFPESAG
                     LSFYQYVWLLWSIKESVYKYRSRANPRLPFAPLKIGVRQLNACSPGNLIAGLATHEDA
                     VLYSRSWLNSEFIATVVSNDRRFQGTRHGVQRIGSPDAASQSAIVRELALTSLARHYP
                     GAALRIEKTPGGPPIVWDGDRPLDLPLSLAHHGHYIAFAYRLPSAGIPPPASPNSSAA
                     IKSAAN"
     gene            complement(1124..>2027)
                     /locus_tag="VKU83_08865"
     CDS             complement(1124..>2027)
                     /locus_tag="VKU83_08865"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI93707.1"
                     /translation="LPALPQMKAIRYKDEVFPSIAVEKNLCYSPGDPVSAHRFDWYEP
                     QNDSTLTRPLVIWMHGGGFRFGSKNMKSIRIWCRFFARRGYVCAAINYRLGKKSLNFG
                     FDDLVKNCYQALGDVRLAIAWFKANAIRLRIDTSRIILAGNSAGGMLALQVAYGTDAA
                     LLQLLHNPDSASASHHVDPGRIAAVINFWGGIFDTAWLRNARVPIVSVHGTEDPIMPY
                     GHKSFPLFGSGDIQRVAGSLGIPSRLKKYPGYSHELQKHFNPLIVTASTRRRQLEAAQ
                     FAADFIAAELFGEAGGGMPAEGSR"
BASE COUNT          474 a          552 c          561 g          440 t
ORIGIN      
        1 tcccgccggt caattccttg aaatcgatga tcggccactc gttgagcgcg gcgggatcga
       61 ttgaatcggt ggcggctgtt ttacagatcg tgcatgggtt cattcacccg aatatcaacc
      121 tggaagatcc gaatcccgag attatgaagc aggtcggccc ggactgcctg ccggtgacga
      181 tgatcgaaaa ggagttgaga accgtggcaa aagcgaattt tggtttcggc gatgtaaacg
      241 cgtgtttaat attttcaaaa tatgattaaa atggagagac gagaaatctt ggacgaactt
      301 aagaaggtat tgtaccctta cacggaaaat aaagaattgt tggaaaacat ccatgaaaat
      361 accagccttg ttaaggactt aaagattaat tcagctaacc tggtcgatat catcattgac
      421 gcggaggcaa aatacaatat tgagatcgat ttcgactcag ctgaaaagat cgtcaccgta
      481 ggcgattgcg tcaacgctat cgaggagaaa gtcaactgaa cgccatcatg agcgccggta
      541 acgatattgt agctttggcc gcaacggaca gtgacaggac ctgccggtat cgtttcttct
      601 cgcgtattat tacacaacac gaaaaacagc aatacttcgg ttttccggaa agtgccggat
      661 tgtctttcta ccaatatgtc tggctgcttt ggtcgatcaa agaatcggtg tacaagtacc
      721 ggagccgggc gaacccccga ctcccttttg cgccgttaaa gatcggcgtc cggcagctga
      781 acgcatgttc ccccggcaac cttatcgcgg gcctggcgac ccacgaggac gccgttcttt
      841 attccaggtc ctggctgaac agtgaattca ttgccaccgt cgtcagcaac gaccgcagat
      901 ttcaagggac gcggcatggc gttcagcgga tcggttcgcc ggacgccgca agtcaatcgg
      961 ccatcgtccg cgagctggcg ttgacctcgc ttgcccgcca ttacccaggc gctgcgctcc
     1021 ggatcgaaaa gacgccgggc gggccaccga tcgtctggga cggcgaccgg cccctggacc
     1081 tgcccctttc gcttgcccac cacggccatt acattgcttt cgcctaccgg cttccttccg
     1141 cgggcatccc tccaccggct tcgccgaaca gctccgcggc tataaagtct gcggcgaact
     1201 gagccgcttc caactgccgg cgtcgggttg atgcagtgac aatcaacgga ttgaaatgct
     1261 tttgcagttc gtgggaatac ccggggtatt tcttcaaccg gctcgggatc ccgagggaac
     1321 ccgcaacccg ctgaatatcg ccgctgccaa aaaggggaaa agatttatgc ccataaggca
     1381 tgatcggatc ttccgtgcca tgcacggata cgatcggcac ccgcgcgttc cgcagccagg
     1441 cggtatcgaa aatgccgccc cagaaattga tgacggcggc gatccggccc gggtcaacgt
     1501 gatgcgaagc actcgccgaa tccgggttgt gcaggagttg gagcaaggcg gcatcggtgc
     1561 cgtaggcaac ttggagcgcc agcatgccgc cggccgaatt tcccgcgagg atgatccggc
     1621 tggtatcgat ccgcaggcga atggcattgg ccttgaacca ggctatcgca agcctgacgt
     1681 ccccaagggc ctggtagcag ttctttacca ggtcgtcaaa cccaaaatta aggctctttt
     1741 ttccgagccg gtaattgatc gcggcgcata catatccccg gcgcgcgaaa aagcgacacc
     1801 agatccggat gcttttcatg ttttttgatc cgaacctgaa acctccccca tgcatccaga
     1861 taaccagggg acgggtgagc gtgctgtcgt tttgcggttc gtaccagtcg aagcgatgcg
     1921 cggagacagg atcgcccggt gaatagcaga ggtttttttc aacggcgatg gaagggaaaa
     1981 cttcgtcctt atagcgaatg gccttcattt gcggtagggc gggaagc
//
LOCUS       DATGKE010000516         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_426057, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000516 DATGKE010000000
VERSION     DATGKE010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426057"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1505
                     /locus_tag="VKU83_08870"
     CDS             183..1505
                     /locus_tag="VKU83_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93708.1"
                     /translation="MKPGIHFSTKQLSSPRGAGRPVAVLILVLLFSSMRVFAQGNLSP
                     YSQMGLGDLDFGYYNRTSGLANTGIAYRSNRFLINNNPASFSGLTDQYFTMETALRGE
                     YSTYAGNQVNVASTESGDITFRKLILGIKLTHNWGSSIGILPYSTQNYEFIAPYNIPG
                     TTIIIADHYYQGHGSVNRAYWANSYEFFHHLSIGVDASYIFGQLNQNDIIQSSGTGAT
                     LVSTTNNTNLQNVYLTYGAQLYGRVGRNWNYSLGGTYSAKAALLAASTKEVLNNDSVM
                     LQNIQNQEGYLYLPQSWGIGIALTNRQKYTFLADYRYQNWNGVAAENGYPGQGYAIAS
                     AERASIGFEVSKKKVYGQVITEASYLQAGLYYENSYLQINGKQIKDYGITCGFGINSP
                     KSPLAYSVVFQYGIRGTTANDLIRQNYADITFLINFGQIWYTKGRKFD"
     gene            1514..2278
                     /locus_tag="VKU83_08875"
     CDS             1514..2278
                     /locus_tag="VKU83_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93709.1"
                     /translation="MAPSSLAGSDWYSPIKGIPVAGTLNCIVVEDNSKQSATLKEYIA
                     KVPGLSFSGRCESSADLLQLVPQFRANLIFWDIRLLDNRVMARLKDAGYYPLIICIAD
                     KQEQEERPEIDVFTYLIRPLSFDRFLSAMNKVRDYLSTSAYIHHRNNRFVFVKSEYKI
                     IKVKFEDILFCEGMKDYTQIYIRGRSEPILTLNNLKSFSSKLPAEEFIRVHRSYIISL
                     AHIDSIARNEIYIGKKIIPIGDSFKDDFYQIIEWNS"
     gene            2316..>2847
                     /locus_tag="VKU83_08880"
     CDS             2316..>2847
                     /locus_tag="VKU83_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93710.1"
                     /translation="MTNRASVRIAILDLYEGQPNQGMRCIRELIADFGGAHRLRFEVD
                     EFDVRIRNEVPDLSYDIYISSGGPGSPLDSVNSVWEEAYFGWLRKIEASNRSSAKRAR
                     KQVLFICHSFQLICRHYGIAQVTNRRSTAFGVFPVHLLDGAAEEPVFAGLGDPFYAVD
                     SRSYQVIQPNRRRIAEM"
BASE COUNT          688 a          860 c          666 g          633 t
ORIGIN      
        1 accaatacca accagggggc aagccaggcc ggattgatgc tcagcattcc gagcccggcc
       61 aatcttggct cgttccggcg gctggcgatc gccgaccaaa gctttccgac ccagcagcgg
      121 attgttctca gtgtatatta catctcactt tacccacatc aataactaac tcacaaaatc
      181 ggatgaaacc gggcattcac ttttccacaa aacagctgtc gtccccgcgg ggcgccggcc
      241 ggcctgttgc ggtccttatt ctggtgctcc tgttttcctc catgcgggta tttgcccagg
      301 gcaacctttc accctattcg caaatggggc tgggcgacct cgatttcggc tactacaacc
      361 ggacttccgg tctcgccaac accggcatcg cctaccgctc gaaccgcttc ctgatcaaca
      421 ataacccggc aagcttcagc gggctcaccg accaatattt tacgatggaa accgccctcc
      481 gcggcgaata ctcgacctat gccggcaacc aggttaacgt ggcaagtacc gaatcggggg
      541 acatcacttt ccggaaactc atcctcggaa tcaaacttac ccataactgg ggttccagca
      601 tcgggatctt gccttacagc actcagaact acgaattcat cgcgccctac aatattccgg
      661 gaacaacgat catcattgca gaccattatt atcagggaca cggaagcgtc aaccgtgcgt
      721 actgggccaa ctcctacgaa ttcttccatc acctgtccat tggcgtcgat gcctcctaca
      781 ttttcggcca gttgaaccag aacgatatca tccagtcgag cggcaccggc gcgacactcg
      841 tctccaccac caataacacc aaccttcaaa acgtttatct cacctacggc gctcagttgt
      901 acggccgcgt gggcaggaac tggaattatt ccctcggcgg tacctacagc gcaaaggcgg
      961 cattgctcgc cgccagcacc aaagaggtgc tgaacaatga ctctgtcatg ttgcagaaca
     1021 tccaaaacca ggaaggctac ctgtaccttc cccagtcctg gggtatcggc atcgccttga
     1081 cgaacaggca gaaatatact ttcctggcag attaccgcta tcagaattgg aacggcgtcg
     1141 cggccgagaa tggctacccg ggtcaggggt atgctattgc cagcgctgaa cgggcgtcca
     1201 tcggcttcga ggtcagcaaa aaaaaggttt atggtcaggt aattaccgag gccagttatc
     1261 tccaggcggg tctctattac gagaacagct acctccagat caacggaaaa cagatcaaag
     1321 actacggcat cacctgcggc ttcggcatca actcacctaa gtcgccgctg gcatattccg
     1381 tggtgttcca atatgggata aggggcacga cagcgaatga tcttatccgc cagaactacg
     1441 ccgatattac cttcttgatc aactttggcc agatctggta cacgaaagga aggaaatttg
     1501 attaatttac agcatggcac cttcctcgct tgcagggtcc gactggtatt ctccgatcaa
     1561 gggcattcct gtagcgggga ccttgaattg catcgtcgtc gaggataaca gcaaacaaag
     1621 cgccaccctc aaagaataca tcgctaaggt ccccggcctc tccttctcag gccgctgcga
     1681 atcctccgcc gatctgctcc agcttgtccc tcaattccgc gcgaatctta tcttctggga
     1741 catccgcctg ttggacaaca gggtcatggc acggttgaag gatgcagggt attatcccct
     1801 gatcatatgt atcgccgaca agcaggaaca ggaagagcgg ccggaaatcg atgtcttcac
     1861 atacctcatc cgccccctgt cattcgaccg tttcctttct gccatgaaca aggtccggga
     1921 ttacctgtcg acatctgcgt atattcatca ccggaacaac cggttcgtgt tcgtcaagtc
     1981 ggaatacaag atcattaaag taaaattcga agatatcctc ttctgcgagg gtatgaagga
     2041 ctatactcag atctacataa gaggcagatc cgaacctatc cttactctca acaatctgaa
     2101 gtccttctcc tctaagcttc ccgccgagga gttcattcgc gtgcaccggt cctatatcat
     2161 ctctctcgcc catatcgact ccatcgcccg caatgagatc tacatcggaa aaaagatcat
     2221 tccgatcggt gacagtttca aagacgattt ttaccagatc atcgagtgga actcctagac
     2281 cgctcctccc ggcaaaaaaa tcctaaattt gcggcatgac aaatcgcgcg agcgtccgca
     2341 ttgccatact cgacctctat gagggccagc ccaaccaggg aatgcgttgc atccgcgaac
     2401 tgatagcgga tttcggcggc gcccaccgcc ttcgttttga agtcgacgaa ttcgacgtac
     2461 ggatcaggaa cgaagtcccg gacctgtcct atgacatcta tatttccagc gggggacccg
     2521 gcagcccgct cgattccgtt aacagcgtct gggaggaggc ctacttcggc tggctaagga
     2581 agattgaagc cagcaaccgc agctctgcaa agcgcgctcg caaacaagtc ctgttcatct
     2641 gccattcctt ccaactgatc tgccgccatt acggtatcgc tcaagtaacc aaccgccggt
     2701 ccaccgcctt tggcgtattc cccgttcacc tcctggacgg cgcggcggaa gagcctgtat
     2761 ttgccggact tggcgatcct ttctatgccg tcgatagccg cagctaccag gtcatacagc
     2821 ccaaccgccg gcggatcgct gaaatgg
//
LOCUS       DATGKE010000517         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_639644, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000517 DATGKE010000000
VERSION     DATGKE010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639644"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VKU83_08885"
     CDS             complement(<1..714)
                     /locus_tag="VKU83_08885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HLI93711.1"
                     /translation="MKSRIILFAVFSAVAFVVSAQRSKIPVFVSGTDGFASFRIPAII
                     RTPDNRLLAFCEGRVNGSADFGNIKIVMKRSSDEGKTWTTLQVVASNDTLQAGNPAPV
                     MDLTDPAYPKGRIFLFYCTGNRTEGTIRKGQGVREVWYKTSTDDGANWSKPVNITVQT
                     HRPHQPAVNPAYNFSEDWRCYATTPGHAIQLVGGAYAGRILVPMNYSRGDPQAHFGDF
                     AAADFYTDDHGRSFHLGQDV"
     gene            complement(711..2210)
                     /locus_tag="VKU83_08890"
     CDS             complement(711..2210)
                     /locus_tag="VKU83_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HLI93712.1"
                     /translation="MRAFCLFLMLAALQAKAQLRLPPFFSDNMVLQRGRPIHFWGRGV
                     SGERVTIRFSGATRTVLVKPDSSWSLFFPRQPANPIAQNIIIASRSESLTLKNILIGD
                     VWICSGQSNMEFPFEREAHAATEYARAAEPVIRFANPAPAGRYVYGVRYTDSLTRRLN
                     ADSFYRWDGWQNCDSSTIGPMSAVAYYFAKKLVAETHIPIGLINVSIGGAPLETFISR
                     SALAASPRFAAKVKAGNWLDNDALSVWARERGRQNIAGAYRYGDDRGPNHAYKPGFAF
                     DCGLRPLLPMPVKGILWYQGESNSLESDRVKEYPALLHLMIDDYRAGWRDPDLSFYWV
                     QLSSIDTVNYQSRYWPEFRDGQRQLLAAIKHGGMAVCSDIGAKENVHPTNKKDVGERL
                     ARWVLYQDYHLPVIPSGPLPLRARYIHGEVVVDFRYGDGLRTSDGGGLREISLDGKHD
                     TYAMIDGATIRIPAAEEPASVYYGWQPFSRGNLVNAEGLPASTFKIDVQ"
     gene            complement(2207..>2407)
                     /locus_tag="VKU83_08895"
     CDS             complement(2207..>2407)
                     /locus_tag="VKU83_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93713.1"
                     /translation="DWHRKIHAYAYSAFHDPVHGEWFGYLHRDGSLAQTAKGNLYKGP
                     FHLPRQEWYCMRILESFLNDHS"
BASE COUNT          528 a          674 c          707 g          498 t
ORIGIN      
        1 gacatcctga ccgagatgga agctgcggcc gtggtcatca gtataaaaat cagcggcggc
       61 aaaatcgccg aaatgcgctt gcggatcgcc gcgcgaataa ttcatgggca caaggatgcg
      121 gccggcatat gccccaccaa ccagttggat cgcgtggccg ggcgtcgtcg cataacaacg
      181 ccagtcttcg gaaaagttat aggcggggtt tacggcgggc tgatgcggcc tgtgcgtctg
      241 cacggtaata tttaccggtt tgctccagtt ggcgccatca tcggtcgacg tcttatacca
      301 tacctcccgt acgccctgtc cctttcggat cgtcccctcc gtacggttgc ccgtgcaata
      361 gaacaggaag atcctgcctt tgggataagc agggtcggta aggtccatta cgggggccgg
      421 attccccgct tgcaacgtgt cgttgctcgc caccacctgc agcgtagtcc aggtcttgcc
      481 ctcatcggaa ctgcgcttca tcacgatctt tatattaccg aaatcggcgg agccgttaac
      541 gcggccctcg caaaaggcca gcagccggtt atccggcgtg cggatgatcg ccggaatacg
      601 gaaggaggca aagccgtcgg taccggaaac gaacaccggt attttactac gctgggccga
      661 gaccacaaaa gcgacagcgg agaaaacagc aaacaggatg atcctggact tcattgtacg
      721 tctattttaa aagtggaagc gggcaatcct tcggcgttga cgaggttgcc gcgggagaaa
      781 ggctgccagc cgtagtaaac gctcgcaggt tcttctgcgg cgggtattcg gatggtcgcg
      841 ccgtcgatca tggcataggt gtcatgtttt ccatccaggg aaatttcccg gagaccgccg
      901 ccatccgaag tgcgtagtcc gtccccatag cggaagtcaa ccaccacttc gccatggata
      961 taacgggcac ggagcggtaa cgggcccgaa ggtattaccg gcagatggta gtcctggtac
     1021 agcacccacc gggcgagtcg ttcgccgacg tcctttttgt tggtagggtg cacattctct
     1081 ttcgcaccga tgtcgctgca tacagccatg ccgccgtgtt tgatagcagc cagcagctgg
     1141 cgttgcccgt cgcgaaattc gggccaataa cgtgactgat agtttaccgt atcgatagag
     1201 gacagctgca cccagtaaaa agacaggtca gggtctcgcc aaccggcgcg gtagtcgtcg
     1261 atcatcaagt gcaggagggc tggatattcc tttacccgat cggattcgag agagttgctt
     1321 tcgccctggt accaaaggat gcccttcacc ggcatcggca gcagcggccg caggccgcag
     1381 tcgaaggcga agcccggctt gtaagcatgg ttcggacctc ggtcatcgcc gtagcgatag
     1441 gcgccggcga tgttttgccg gccccgctcc cttgcccaaa cggagagggc atcgttatcc
     1501 agccagttgc ctgccttgac ttttgcggcg aatcggggac ttgccgcgag ggcggaacgg
     1561 ctgataaagg tttccagcgg cgcaccgccg atagagacat tgatcagtcc gatcgggata
     1621 tgcgtctctg ctactagctt cttcgcaaaa taataggcca cggcgctcat cggccctatc
     1681 gtgctgctgt cgcagttctg ccagccatcc caacgataaa agctgtccgc gttcagccgg
     1741 cgggtcagcg agtcggtgta tcgaacaccg tatacatatc tgccggcagg tgcgggattg
     1801 gcgaaccgga tcacgggctc ggcggcacgc gcgtattccg ttgcggcatg cgcctctctt
     1861 tcaaatggaa attccatatt ggattgcccc gaacaaatcc aaacatcacc gatcaggatg
     1921 ttttttaggg tcaggctttc acttcggctt gcgataataa tattctgcgc aatgggattg
     1981 gcgggttgcc ggggaaagaa caggctccaa ctggagtcgg gcttcaccag gacggttcgg
     2041 gtggcgccgg aaaaacgaat ggtcacgcgc tcgccggaaa cgccgcgacc ccaaaaatgg
     2101 atcggccgcc cgcgttgcag gaccatatta tccgaaaaga acggcggcag ccgcagctgg
     2161 gctttagcct gcagcgcggc cagcatcaga aaaagacaga aagctctcat gagtgatcgt
     2221 ttaaaaaaga ttcgaggatc cgcatgcaat accattcctg ccgcggcaga tggaagggcc
     2281 ccttgtagag attgcctttg gcggtttgag ccaggcttcc gtcccgatgc agataaccaa
     2341 accattcgcc gtgcacgggg tcatgaaaag ctgaataggc ataagcgtgg atcttccggt
     2401 gccagtc
//
LOCUS       DATGKE010000518         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1464690, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000518 DATGKE010000000
VERSION     DATGKE010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464690"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /locus_tag="VKU83_08900"
     CDS             complement(<1..2316)
                     /locus_tag="VKU83_08900"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI93714.1"
                     /translation="MGKHVIFWLLLGISYATPSFSQSKTRGSVRGAVADSAANTAPLP
                     NATVSIRPVGGDSTTAEYAVSNKQGAFLFRGLPVGRYFVLITYEGFAHVGRDVQITDS
                     NANVDLGTFYLQHSNDLLEAAIVQRPPMGIKKDTVEYNAGSFAVKPNGTAEDLLNKMP
                     GITVDHSGTVTAQGETVQRVMVNGKRFFSDDPKLATRNLPPDIIDKIQVFDDLSDQSK
                     FTGFDDGNRVKTINIITKKNTNKGYFGKFVAAGGTNEDYDESLNLHRFDGNRQISLLG
                     QGNDINKQNFTPQDIFGGGGGRRGGGPSASTSYQSNGVTTVWAGGANYRNTLGTNPNH
                     STDIYGSYFYNYQHVLQTQTDSSISPVPNDYGADSSLVSSGGNTAISRISSHRVFFNV
                     ESRIDSNNSIIFRPNITFERSTPNGSSFSTETDNHGNLVNSLIGHTSSENAGFQISNS
                     NLQIRHRFAKPYRTISLDINTTANINNGSGYTYSVNNFYFPLKRVDTINQFYNDSVHA
                     WTLTPTLSYTEPIAKDQILQFTYSHTYNTSTSINNTYDYVDLQKSYSHFDYLFSNSYK
                     FKSNADNAGLYWRIQHPTYNLSVGSGIQWTNFTSINTTKDTTVARNYVNFTPSVNFHY
                     NMSNTRHLRVFYRGSTGTPSPSQLQPLVTTSDSVNFQLGNPALKPQFTHSLRVLYADF
                     DPSTQHVMFATVNASTIVNDIQNVSYYNKEGGKNSTYANLNGTWNVSAFFNYGFPLKH
                     PKSNLNFMTNVQYSQSQTGLATDSASYLTGQF"
     gene            complement(2487..>2836)
                     /locus_tag="VKU83_08905"
     CDS             complement(2487..>2836)
                     /locus_tag="VKU83_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HLI93715.1"
                     /translation="ERERAIQSFQNDDSCRVFLISLKAGGVGLNLTAADYVYIVDPWW
                     NPAVEQQAIDRTHRIGQTKNIFAYRMICKDTIEDKILQLQEKKRILAKDLIADDDGFV
                     KSLTRADVEYLFS"
BASE COUNT          587 a          689 c          860 g          700 t
ORIGIN      
        1 gaactgaccg gtcagataag acgccgagtc ggtcgccagg cctgtctggc tttgcgaata
       61 ctgaacattg gtcatgaagt tcaggttgga tttgggatgt ttcagcggga agccgtagtt
      121 gaagaacgcg ctcacattcc aggtcccgtt caggttggca taggtgctgt tcttgccgcc
      181 ttccttatta taataagaga cattctgaat atcgttgacg atcgtactgg cattaacggt
      241 tgcaaacatc acgtgctggg tgctgggatc gaaatccgcg tacaggacgc gcagcgaatg
      301 cgtgaactgg ggcttcagcg cgggattacc cagctggaag ttgacgctgt ccgaggtcgt
      361 gaccaggggc tgcaactggg aaggggatgg tgttccggta ctaccccggt agaagacgcg
      421 gagatgccgg gtgttggaca tattataatg gaagttcacc gaaggcgtaa agttgacgta
      481 gttgcgggcc accgtcgtat ccttggtggt attgatgctg gtgaaattcg tccattggat
      541 tccggacccc accgacaggt tatacgtggg atgctggatt cgccaataca gaccggcatt
      601 gtccgcgttc gacttgaatt tgtatgagtt gctgaacagg tagtcgaaat gagaatagga
      661 cttctgcaaa tcgacatagt catacgtatt gttgatcgac gtgctcgtgt tataggtatg
      721 gctataggtg aattgcagga tctggtcctt ggcgatgggt tccgtatagg agagtgtcgg
      781 ggtaagggtc caggcgtgca cggaatcgtt atagaactgg ttgatggtgt ccacccgctt
      841 cagcgggaaa tagaaattat tcaccgaata cgtgtagccc gagccgttat taatgttggc
      901 ggtcgtattg atatccagcg aaatggtgcg gtacggtttg gcaaaccggt gccggatctg
      961 gaggttcgag ttgctgatct ggaacccggc gttctcgctg gaagtatggc cgatgaggct
     1021 gttgaccaga ttgccgtgat tatcggtctc ggtggagaac gaagacccgt tcggggtgct
     1081 tctttcgaag gtgatgttgg gtctgaagat aatagagttg ttactgtcga tgcggctttc
     1141 cacgttgaag aaaacgcggt ggctcgagat gcgggagatg gcggtattac ccccgctgct
     1201 caccaggctc gagtccgcgc cgtagtcgtt gggaacgggg gagatcgaac tatcggtttg
     1261 cgtttgcaag acgtgctgat aattgtagaa atagctgccg tagatatcgg tcgaatggtt
     1321 ggggttagtc ccgagggtat tccggtagtt tgcgccgccc gcccaaacgg tcgttacccc
     1381 gttggactgg tagctggtac tggccgatgg gccgccgccc cttctgccgc cgccgccgcc
     1441 gaagatgtcc tgcggcgtaa agttctgctt gttgatatca ttcccctgcc cgagaaggga
     1501 gatctgccgg ttgccgtcga aacggtgcag gttgaggctt tcgtcataat cttcgttcgt
     1561 gccgccggcc gccacgaatt tcccgaaata gcctttgttg gtattctttt tcgtgatgat
     1621 attgatcgtc tttacacggt tgccgtcgtc aaatccggtg aacttgctct ggtcgctcag
     1681 gtcgtcgaaa acctggatct tgtcgatgat atcgggcggg aggttgcggg tcgccagttt
     1741 cggatcgtca ctgaaaaagc gcttgccgtt caccatgacc cgctgcacgg tctcgccctg
     1801 ggcggtgacg gtgccgctat gatcgaccgt gatcccgggc attttgttga gcaggtcttc
     1861 agccgtccca ttgggtttca cggcaaagga gccggcattg tattccacgg tgtccttctt
     1921 tatccccatc gggggacgct gcacgatcgc cgcttccagg agatcattgg agtgttgcag
     1981 gtagaaggtt cccaggtcaa cgttggcatt gctgtcggtg atctgcacgt cccggccgac
     2041 atgggcaaag ccttcatacg tgatcaggac aaaataccgg ccgaccggga gcccccggaa
     2101 aaggaaagcg ccctgcttgt tggataccgc atattcggcg gtggtggaat cgccgcccac
     2161 cggccggatc gaaacggtcg cgttgggcaa cggcgctgta ttggccgcgc tgtcggcaac
     2221 agcgccccgg acggagcctc ttgtcttcga ctgtgaaaac gaaggagttg cataagagat
     2281 tcccagaagt agccaaaaaa taacatgctt gcccatatcc agccggttgt gtgaaatgct
     2341 gcactaattt acgacgcagt cgtttggccc gtaaggacat acattaccag tggcaaaata
     2401 gcctgcccat agggccagca aacgaccggg gaagcgattc tgtcggtcaa tgcgagattg
     2461 taaccggcat tgagcgaagc ctgccattaa gagaagaggt attccacgtc cgcgcgggta
     2521 aggctcttta caaaaccatc gtcgtcggcg atcaggtcct ttgcaagaat gcgcttcttt
     2581 tcctgcagtt gcaggatctt gtcttcgatc gtgtccttgc agatcatgcg gtaggcaaaa
     2641 atgtttttgg tctgcccgat gcgatgggtc cggtcgatag cctgttgctc gactgcagga
     2701 ttccaccatg gatccacgat atacacgtaa tcggccgccg tcaggttgag acctacgccg
     2761 ccggctttga gggagatgag aaagacccgg cagctgtcgt cgttctgaaa gctctgtatc
     2821 gccctttccc gttccg
//
LOCUS       DATGKE010000519         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1363762, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000519 DATGKE010000000
VERSION     DATGKE010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363762"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1441)
                     /locus_tag="VKU83_08910"
     CDS             complement(80..1441)
                     /locus_tag="VKU83_08910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLI93716.1"
                     /translation="MKNHHSFKVFIVEDDVWYGSMLQHYLSLNPEYEVKRFESPRDFF
                     AQLHENPEVVTLDYSLPDCDGAEVLKKIKEHNPDIRVIVISGQEDVATAINLLKNGAF
                     DYIVKDDDTKDRLWNSILHLREISNLKQEVETLKSQVTRKYDFSQMILGKSDQIQKVF
                     SLIEKAAKTNITVSVTGETGSGKEMIAKAIHYNSDRHKMPFVAVNVAAIPKDLLEAEL
                     FGHEKGAFTGAVTRRIGKFEEADKGTLFLDEIGELDINLQAKLLRVLQEREITRIGSN
                     VVTKIDVRIIVATHKNLLEEVKNRNFREDLYYRLIGLPIYLPPLRERGNDILILSKHF
                     IDAFCRENKIEKKTLASDAQQKLLQYPFPGNVRELKSVVELAVVMADDEMIQAEHITL
                     NTSASIADLLNKEMTLKEFEIQILQHYLDKYERDVLLVAKKLDVGKSTLYRMIQNGEL
                     KMK"
     gene            1646..>3397
                     /locus_tag="VKU83_08915"
     CDS             1646..>3397
                     /locus_tag="VKU83_08915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HLI93717.1"
                     /translation="MSINRDKLRQQFEEEYLKLNEQQRLAVDSIEGPVMVIAGPGTGK
                     TQILAARIGKILLETDTAPGNILCLTYTDAGAIAMRRRLVSLIGPDAYKVHVYTFHSF
                     CNDIIQENLPLFEKTALDPISDLERIQLFKSLIDGWPKNHPLKRYRGDVYFEMNNLQG
                     LFSTMKREGWTAAFVNERIDRYLQELPSRDEYIAKRATKEFRKGDVRTDKIAEETERM
                     SKLRAAVGEFQPFQDLMRRQNRYDFDDMINWVIRAFNENKDLLASYQERYQYILVDEY
                     QDTSGTQNKLVSLLAGYWEQPNVFVVGDDDQSIYRFQGANVENMLEFAHDFQKDLLTV
                     VLTNNYRSTQPILDISRTLIERNRERLVNQIPGLSKLLVSSHPRVSQFQDPPRLNEYL
                     SPRQEMTHITLQVEQLLASGVEPGRIAILYKENKYGEELARFLQLKKIPVYSKRSLDL
                     LAVPLAQKILLLLRYLGAEHDAPYGGDEMLFEILHFDCWGVPPIEIAKLSVEVADRQF
                     SDQRTSLRRLLYEKSNRPPRDLFDSGLPEGLKRASRVLEKLTGDVVNVTLQTLFENCI
                     RETGILGLVLASADKIFG"
BASE COUNT          757 a          895 c          920 g          825 t
ORIGIN      
        1 ccttatccag aatctcctca aaaaagctgc tctctaacgg cattccaatg tcggtttatc
       61 taaggggtca tcccatctgt catttcatct tcagctcacc gttctggatc atgcgataca
      121 gcgtggactt gccgacgtcc agtttcttcg cgaccagcaa aacgtccctt tcgtatttat
      181 ccaggtaatg ctgcaggatc tggatctcaa actctttcag ggtcatttcc ttgttcagca
      241 gatcggcgat gctggcagac gtgttcagcg taatatgctc ggcctggatc atttcatcgt
      301 ctgccataac cacggccagt tccaccaccg atttcagctc gcggacattt ccgggaaagg
      361 gatattgcaa taatttctgt tgtgcgtccg acgcaagggt cttcttttcg atcttgttct
      421 cgcggcaaaa ggcgtcgatg aagtgcttgg aaagaatgag gatatcgtta ccccgttcgc
      481 gaaggggcgg caggtatatc gggaggccga tcaggcggta atagaggtct tcgcggaaat
      541 tccggttctt cacttcttcg agcaggttct tatgggtggc cacgataatg cggacatcga
      601 tctttgtcac gacgttgctg ccaatgcggg tgatctccct ttcctgcagc acccggagaa
      661 gctttgcctg aaggttgatg tccaattcgc cgatctcatc gaggaagaga gtgcctttgt
      721 ccgcctcctc gaatttgccg atgcggcggg ttactgcgcc tgtaaaggcg cccttttcat
      781 gcccgaacaa ctcagcttcg agcaggtcct tgggtatcgc ggcgacgttg acggcaacaa
      841 agggcatttt gtggcgatcg gagttataat ggatcgcctt ggcgatcatt tcctttccgg
      901 acccggtttc gccggtgacc gacaccgtta tattggtctt ggcggctttt tcgatcaggg
      961 agaatacctt ctgtatctgg tccgattttc cgaggatcat ctgtgaaaaa tcgtactttc
     1021 tcgtgacctg gcttttgagg gtttcgactt cctgtttgag gttgctgatc tcccgcaggt
     1081 gtaagatgct gttccagagg cggtctttgg tatcgtcgtc cttgacgata tagtcgaaag
     1141 cgccgttctt gagcaggttg atagccgtcg ccacgtcttc ctggccggag atgacgatga
     1201 cgcggatgtc cgggttatgc tccttgatct tcttgagcac ttccgcgccg tcacaatccg
     1261 gcagggaata atccaatgtt acaacttccg ggttttcatg caattgggca aaaaaatcgc
     1321 ggggagattc aaatcttttt acttcgtatt cgggattcag ggacagataa tgctgcagca
     1381 tggatccgta ccatacatca tcttcgacaa taaacacctt gaaagagtga tggtttttca
     1441 tatttgcgtg atcattggat aggaatacaa cgctgaatcg gcgattttat tcccagttta
     1501 agacattttc caatcccttt ttgctttttt gggccggccg cggtcctttc cgcccctttc
     1561 gggacccgca ccctgcggtc ggaccccgtc gttcgcagcg gttcgtcccc tgctgcggtt
     1621 tcctgccgat tcttaccttt acaacatgtc gatcaatcgg gacaagctca gacagcaatt
     1681 cgaggaggag tacctgaagc tcaatgagca gcagagactg gccgtggaca gtattgaagg
     1741 gccggtcatg gtcattgccg gacccggcac cgggaaaacg cagatcctgg cggccaggat
     1801 tggcaaaatc ctgcttgaaa ccgatacggc ccccgggaat atcctctgcc ttacgtacac
     1861 agatgccggc gccattgcga tgcgccggcg gttagtatcc cttatagggc ccgatgccta
     1921 caaggtgcat gtttacacct tccattcttt ttgcaacgat attatccagg agaacctgcc
     1981 gcttttcgaa aaaacggctt tagatccgat ttccgatctg gagcgaatcc agcttttcaa
     2041 atccctgatc gacggttggc caaagaacca cccgcttaaa cggtatcgcg gggacgttta
     2101 ttttgagatg aataacctgc agggcctgtt ttcgaccatg aaacgggaag gctggacagc
     2161 agccttcgtc aacgaacgga tcgaccggta cctgcaagag cttccttccc gggatgaata
     2221 cattgccaag cgcgcaacaa aagaattcag gaaaggcgat gtccgcacgg acaagatcgc
     2281 cgaggaaaca gagcggatgt ccaagctccg ggcggcggtc ggggaatttc agccgttcca
     2341 ggacctgatg cgccggcaaa accgttatga ttttgacgac atgatcaact gggtgatccg
     2401 ggcattcaat gaaaacaagg atttgctcgc aagctaccag gagcgctacc aatatatact
     2461 cgtagatgaa taccaggata caagcggtac ccagaataag ctggtcagcc tgctggccgg
     2521 gtattgggag cagcccaacg tttttgtcgt cggcgatgac gatcagagca tctatcggtt
     2581 ccagggcgcc aatgtggaga atatgctgga gttcgcgcac gactttcaga aggacctgct
     2641 gacggtcgtc ctgaccaata attaccggtc cacccaaccc atcctcgata tctcgcggac
     2701 cctgatcgag cgcaaccggg aacgactggt aaatcaaatt ccgggacttt cgaaactgct
     2761 ggtttccagt catccgcggg taagccaatt ccaggacccg ccccggctga acgagtatct
     2821 cagcccgaga caggagatga cgcatatcac gctccaggta gagcagctgt tggcttccgg
     2881 cgtcgagccc ggccggatcg ccatcctgta caaggaaaat aagtatgggg aagagcttgc
     2941 gcgcttcctc caattgaaga agataccggt ctacagcaag cgaagcctcg atctgctggc
     3001 ggtgccgctg gcgcaaaaga tcctcctgct cctgcgctac ctcggggcag aacatgatgc
     3061 gccgtatggc ggggacgaaa tgctgttcga aatcctgcat ttcgattgct ggggcgtacc
     3121 gccaatagag attgccaaac tatccgtaga ggtcgcggac cgccagttca gcgaccagcg
     3181 cacctcgctg cgcaggctgc tttacgagaa gtccaaccgg ccgccgcggg acctgtttga
     3241 cagcgggttg ccggagggat tgaaacgggc cagccgggtc ttggaaaagc tgaccgggga
     3301 cgtggtaaac gtaacgctgc aaactctttt tgaaaactgt atccgggaga cagggatcct
     3361 ggggctggtg ctggcgagtg cggacaagat tttcggg
//
LOCUS       DATGKE010000520         4529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_254081, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000520 DATGKE010000000
VERSION     DATGKE010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254081"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VKU83_08920"
     CDS             <1..1237
                     /locus_tag="VKU83_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLI93718.1"
                     /translation="ILNSISTNVWLNLLFAAIFLAFALSFFGLFELTLPSGLSTRIDS
                     RSGLGSLGGIFFMALTLVIVSFSCTGPILGSLLVGALDQNGGGIQLTVAMAGFGLALG
                     LPFALFALFPGWLHSLPKSGGWMDSVKKTFAFVELALMIKFLSNADLVTHSGLLKREV
                     FFAIWILIGIALVLYLFGILRFGHDPRPQKPGRGRLVAGVVFLVFVLYLVPGLTNTRY
                     ANLAFVSGFPPPLSYSIYGSHVSADKGVEADVVNDYNKALALAKQEHKPLLIDFTGWA
                     CVNCRKMEENVWPQPGVKQLIKKDYILVSLYVDDRKRLPDDQQFSFPTSDGSIKAIQT
                     VGDKYATLQSENFRNASQPLYVVISPDEKLMTKPVGYTPNAAEYAQWLQCGLDAFQHE
                     KSMARSKKEPDFSSSGSLN"
     gene            complement(1483..2064)
                     /locus_tag="VKU83_08925"
     CDS             complement(1483..2064)
                     /locus_tag="VKU83_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLI93719.1"
                     /translation="MTLLRKKTDHELIHQFKDGDLHALEALIIRHKDKLYTSILFLVK
                     DKYLAEDIFQDVLIKIIDTIRGGRYTEEGKFLPWAMRIAHNLCVDHFRKVKRTPAIKT
                     SDDRDIFEVLHFTEEGADTKMMKRQSHDRVRQMLDLLPEDQREVIILRHYADLSFKEI
                     AALTNCSINTALGRMRYGLINLRKMMTEKKIAL"
     gene            2442..>4529
                     /locus_tag="VKU83_08930"
     CDS             2442..>4529
                     /locus_tag="VKU83_08930"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93720.1"
                     /translation="MSISGKVKRKKVEKEGPGTAAPPADAIFIKGARVHNLKNVDVSI
                     PRNQLVVVTGVSGSGKSSLTIDTLFAEGQRRYAESLSAYARQFMARMDKPDVDYIKGL
                     CPAIAIEQKVITRTPRSTVGSMTEIYDYLRLLFARIGKTYSPVSGRLVKKDDVTDVVS
                     AIAGLDPADRVLLLTAFRQHKNRQPREELNILLQKGFSRLYAGGETLRIEDLLELEDA
                     ALKRKIPGQGSKSPGAFILIDRLVVKAFDEDDRHRIADSVTTAFYEGEGELLLEVNGE
                     RRLSFSNRFELDGITFEEPVPNLFSFNNPFGACPTCEGFSQVLGIDPDLVIPDKRLSV
                     YEGAVAPWKGEKLGWWRDQFVKAAKKFDFPVHKPIIDLTKQQYRCLWEGNEHVHGIND
                     FFAEVERNLYKVQYRVLLSRYRGRTECPDCHGYRLRKEALYVKIGDRHIGQLCEIPVR
                     DLSKWFDGLSLEPHEQQIARRILLEVRHRLATLENVGLGYLTLSRLANSLSGGESQRI
                     QLTRSLGSNLTNSLYILDEPSIGLHSRDTERLIRVLKELRDLGNTVVVVEHDEMMMRE
                     ADHIIDMGPLASHLGGEVVATGNYEALMANEDSLTGKYLKGELAITPPARPRSWSRSL
                     LIEGARQNNLKDLNVEIPLNVLAVVSGVSGSGKTTLVKQILYPALQKIKGEPADKVGI
                     HKAISGDIDYLSQI"
BASE COUNT         1010 a         1253 c         1210 g         1056 t
ORIGIN      
        1 tatcctcaac agcatttcta cgaacgtgtg gttaaacctt ttgtttgcgg cgatcttcct
       61 tgcttttgcg ctttcctttt tcggcctttt cgagctgacc ctgcccagcg gactgtcgac
      121 caggatcgac tcgcgctccg gtctcggaag cctggggggg atatttttta tggcgctgac
      181 actggtgatc gtctcctttt cctgcaccgg cccgatccta gggagcctcc ttgtcggcgc
      241 gctcgaccag aatggcggcg gcatccaatt gaccgtggcg atggccgggt tcggccttgc
      301 gctagggttg cctttcgcgc tgttcgcgct cttccccggc tggctccatt cgctgcccaa
      361 gtcaggcggc tggatggaca gcgtcaaaaa gacctttgcc tttgtggaac tggccctgat
      421 gatcaaattc ctctccaatg ccgacctggt cacccactcg ggattgctta aaagagaggt
      481 ttttttcgcg atctggatcc tgatcggcat tgcccttgtg ctctacctgt tcgggatact
      541 ccggttcggc cacgacccgc gcccgcagaa gcccggccgc ggccggttgg tagcgggggt
      601 ggtctttctt gtctttgttc tgtacctcgt tccgggcctg acaaacaccc ggtacgcgaa
      661 cctggccttc gtcagcgggt tcccgccgcc cttgtcctac agcatttacg gatcgcatgt
      721 atcagccgac aagggagtcg aagcggatgt ggtcaacgac tataacaagg cgcttgcgct
      781 ggcgaagcag gaacacaaac cgctcctgat cgactttacg ggatgggcct gcgtcaactg
      841 ccggaaaatg gaggagaacg tgtggccgca gcccggtgtg aagcagctga ttaaaaagga
      901 ttatatcctg gtttccctct atgtcgacga ccgcaagcga ctgccggacg accaacagtt
      961 ttcctttccg accagcgacg gaagcatcaa ggcgatccag actgtcggcg ataagtacgc
     1021 cacgcttcag tcagaaaatt tcaggaacgc ttcccagccc ctctatgtcg tcatcagccc
     1081 ggacgagaaa ctgatgacaa agccggtggg ctacacgccg aacgccgccg aatatgcgca
     1141 atggctccaa tgcggccttg acgccttcca gcacgaaaag tccatggcga ggagcaaaaa
     1201 agagcccgac ttttcatcat cgggctccct taattaggcg tcattccgta tcaaccttcc
     1261 ggcaggctta agggcaagtg cttaacaccg gatggagagc cggaatgaca ggctctttta
     1321 tccgcccgcg cttcatccgc ttcatcgcga ccggacaaaa aaaagttcct gccggtgagg
     1381 cagaaacctt ttcagattct ttccaagttt tacccatttg accttttcgt actgccaagt
     1441 gagctaccat cttttccaaa aatgagtaat gaagtaaaaa tgttacaagg caatcttttt
     1501 ttcggtcatc attttacgca gattgatcag cccgtaacgc atcctgccca gcgcagtgtt
     1561 gatactgcaa ttcgtcaggg cagcgatctc tttaaagctg aggtccgcgt agtgccgcag
     1621 gatgatgact tcacgctggt cttccggcag caggtctagc atctgccgga cccggtcatg
     1681 gctctggcgt ttcatcattt tggtgtccgc accttcttcg gtgaagtgca gcacctcaaa
     1741 aatgtcccgg tcatcgctgg tcttgatcgc cggggtcctt ttgaccttcc ggaaatggtc
     1801 gacgcagaga ttgtgcgcga tccgcatggc ccagggaagg aattttccct cctcggtgta
     1861 gcggccgccc cggatggtgt cgataatttt gatcagaacg tcctggaaaa tatcttcagc
     1921 gagatatttg tcctttacta aaaataaaat cgaggtgtac aacttatcct tatgacggat
     1981 gatcagcgct tccagtgcat ggagatcccc gtccttgaat tgatggataa gttcgtggtc
     2041 ggtctttttt cgtagcaatg tcataaactt ctacgttgtt ttgaaggtga taggatatat
     2101 tttggatttt aaagatggtt tggcttttct atgtagaagt aatcaaacga taggcgagta
     2161 ttatatggct tgttagttaa taaatgaaat tcacatttgg ttcttcattg tgcacaacta
     2221 aaatgtgcga atcgacttta aagatataca atttttggaa aaaacaacat tttgggaaat
     2281 tttttgttcc gtaaattctc cgcggccttg ccgaatcata agggttgccc ttattttgtc
     2341 ctacttcgct ggctaatgcg aacaaaaaga taggccgtct ccctgacaat ttttttagta
     2401 aattcgcacc attaattcac gcagcattgt tattttagtt tatgagcatt tcggggaaag
     2461 ttaaacggaa aaaagtggag aaggaaggac ccgggactgc ggcgccaccg gccgatgcca
     2521 tctttataaa aggagcgcgg gttcacaatc tgaaaaatgt cgatgtcagc ataccccgaa
     2581 accaactcgt tgtggtcacc ggcgtctcgg gttccggcaa atcatccctt accatcgata
     2641 ccctgtttgc ggaggggcaa cgccgctatg cggaaagtct cagcgcctat gcccgtcaat
     2701 tcatggcgcg gatggacaag cccgatgtcg actacataaa gggcctctgc ccggcgatcg
     2761 cgatcgagca gaaggtgatc acgcgcactc cccggtcaac cgtgggcagt atgacggaga
     2821 tctatgatta cctgcgcctg ctcttcgcgc ggatcggcaa aacctattcc ccggtatccg
     2881 gccggctggt gaaaaaagac gatgtgaccg atgtggtgtc ggccatcgcc ggcctggatc
     2941 ccgcagacag ggtgctgctg ctgacggctt ttcgtcagca taagaatcgc cagccccgcg
     3001 aagaactgaa catcctcctt caaaagggat tttcccgatt gtatgccggc ggcgaaaccc
     3061 tccggattga agacctgctg gaactggaag acgcggcatt gaaaagaaag attcccgggc
     3121 aaggttcaaa gtcacccggc gcattcatcc tgatcgatcg cctggtcgtt aaagccttcg
     3181 atgaggacga ccggcaccgc atcgcggact cggtgaccac cgcgttttat gaaggggagg
     3241 gggagctcct gctggaagtg aacggcgagc gccgcctgtc tttttccaat cgcttcgaat
     3301 tggacggcat taccttcgaa gaacccgttc cgaacctctt ttcctttaat aacccatttg
     3361 gggcctgccc tacctgcgaa ggattcagcc aggtgctggg gatcgacccc gacctggtta
     3421 tccccgataa acgcctcagc gtctacgagg gcgccgtcgc gccatggaaa ggggagaagc
     3481 tgggttggtg gcgcgaccag tttgtcaagg cggcaaaaaa attcgatttt cccgtccaca
     3541 agccgatcat cgatctgacc aaacaacaat accggtgtct ctgggaaggt aacgagcatg
     3601 tccacgggat caatgacttt tttgcggagg tcgagcggaa tctctacaag gtccagtacc
     3661 gggtcctgct ttcccgttac cgcggccgga ccgaatgccc ggactgccat ggctatcgcc
     3721 tgcggaagga agcgctttat gtaaagatcg gtgaccgcca tatcggccag ctttgtgaga
     3781 tccccgtccg ggacctttcg aaatggtttg acggtctaag cctcgagccc catgagcagc
     3841 agatcgcacg ccgcatcctg ctggaagtcc ggcaccggct tgccacgctg gaaaacgtcg
     3901 ggctcggcta cctgacgctg tcccggctcg ccaactccct gagcggcggt gaaagccagc
     3961 gcatccagct cacccgctcg ctgggcagca acctcaccaa ctccttatat atactggacg
     4021 aaccttccat cgggctccat tcccgggaca ccgaaaggct gatccgggtg ctgaaggagc
     4081 ttcgcgacct cggcaatacg gtcgtcgtcg tagagcatga tgagatgatg atgcgggagg
     4141 ccgatcacat catcgacatg ggccccctgg ccagtcacct cggcggcgag gtcgtcgcga
     4201 ccggcaatta cgaagcgctt atggccaacg aggatagcct gaccggaaaa tacctgaaag
     4261 gcgagctggc cattaccccg ccggcgaggc cgcgcagctg gagccgttcc ctgctcatcg
     4321 agggggcgcg acaaaacaac ctcaaggacc tcaacgtgga gatcccgctg aatgtactcg
     4381 ccgttgtcag cggcgtgagc ggcagcggaa agactaccct cgtcaaacag atcctgtatc
     4441 ccgccctcca gaaaataaag ggcgaaccgg cggataaggt cggcatccat aaggcgatca
     4501 gcggcgatat cgattacctt tcgcaaatt
//
LOCUS       DATGKE010000521         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_842783, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000521 DATGKE010000000
VERSION     DATGKE010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842783"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..>2461)
                     /locus_tag="VKU83_08935"
     CDS             complement(7..>2461)
                     /locus_tag="VKU83_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLI93721.1"
                     /translation="EVGPTSDEEFRTIVAQGGRRAEIYTRLKDLVDRYADQIRTRYPN
                     IPRRVSGYNLVDLLPENGFNVASALIGTESTCVTILSATLKLMPNPKARSLVVLGYPD
                     LYDSGKHVPEILAFKPIALEGIDDLLIDNMEKKGYHTKNLTLLPDGKAWLLVEFGGDS
                     KTESDALGKAMMAKLKARKDPPAMSLFDDPEQETKLWKIRESGLGATAWVPGEPITEE
                     GWEDTAVPPDRVGDYLVALRDLFAKYGWNIPLYGHFGQGCVHCRVPYDLMTPEGLDQY
                     KRFTEEGARLVVSFGGSISGEHGDGQSKADLLEIMYGPELVQAFREFKEIWDPEWRMN
                     PGKITEPYGQLRNLRTGAQYHPMEPETHFHFPEDSKRFSRATMRCVGIGECRRMKEGT
                     MCPSYRVTLEETHSTRGRARILYEMLMGDVVKGWKDEKVKEALDLCLSCKGCKGDCPV
                     NVDMATYKAEFLSHYYKGKLRPRSALAFGMIYWWARVASIAPRFFNFFTQSKALSGFS
                     KWLTGADPHRKIPAFAKQTFKSWFFKRREPATQRPLSDGKRTFGDRRQPGEQPPVILW
                     TDTFNNNFFPHTLQAAVDVLEAAGFRVIVPRASLCCGRPLYDYGMLNKAVRLLRKILD
                     ELKEEIRNGVPVVGLEPSCVAVFRDELTNLFPDDEDASRLKSQTFTLAEFLQKKAPHF
                     RPPVFTHEAIVHGHCHQKAIMKLDAEKELFDKMRLNYRIVDSGCCGMAGSFGYEKGAH
                     YDVSVKVGELVLLPAVRNASRDAFVIADGFSCREQILQQTDRKPMHTAEVLQLALKQG
                     QTSLVTVEKAAAGSGSMPK"
BASE COUNT          452 a          773 c          676 g          560 t
ORIGIN      
        1 cggatgttat ttcggcatcg atccgctgcc ggccgccgcc ttttcgaccg ttacgaggga
       61 agtctgaccc tgcttcaacg cgagctgtaa tacctctgcc gtatgcatgg gcttcctgtc
      121 cgtctgctgc aggatctgtt cgcgacagct gaaaccgtcg gcgatcacga acgcatcgcg
      181 cgaggcattc cgtacggcgg gcagcaggac cagttcaccc accttcacgc tcacgtcgta
      241 gtgcgcgcct ttttcatagc cgaaactccc tgccatgccg cagcaaccgg aatcgacgat
      301 ccgataattc agccgcattt tgtcgaagag ctccttttcg gcatccagtt tcatgatcgc
      361 cttctggtga caatgaccgt ggacgatggc ttcatgggtg aagacgggcg gccggaaatg
      421 cggcgccttt ttctgcagga actccgcgag cgtaaaggtc tggctcttca accgggaggc
      481 atcttcatcg tcggggaaaa ggttggtgag ctcgtcgcgg aacaccgcca cgcaactcgg
      541 ctcgagtcct actaccggca ctccgttccg gatctcctct ttcaattcat ccaggatctt
      601 ccgcagcagc cgcaccgcct tgttcaacat cccgtaatca tatagcggcc ggccgcaaca
      661 aagggatgcc cggggaacga tgacgcggaa accggcggct tccagcacgt ccaccgcggc
      721 ctgcagggtg tgggggaaga agttgttgtt gaaggtgtcg gtccacagga ttacgggcgg
      781 ctgctctccg ggctggcgcc tgtcgccgaa cgttcttttg ccgtcgctca atggtcgctg
      841 cgtcgccggc tcccgccgct tgaagaacca gctcttgaac gtttgttttg caaaggcagg
      901 gatcttccgg tgcgggtccg cgcccgtcag ccacttgctg aagcccgaca gggctttcga
      961 ttgggtaaag aaattgaaga accgcggggc aatggatgcc acccttgccc accaatagat
     1021 catcccgaag gcgagcgcag atctcggccg gagcttcccc ttataatagt gcgaaaggaa
     1081 ttctgcctta taggtcgcca tatcgacgtt gaccggacaa tcccctttgc agcccttaca
     1141 cgagaggcaa aggtcgagcg cttccttcac cttttcatct ttccatccct tgacgacatc
     1201 tcccatcagc atctcgtaga ggatgcgcgc ccggccccgc gtcgaatgcg tctcctcgag
     1261 cgttacccgg tagcttgggc acatcgtccc ttccttcatc cttctgcatt cgccgatgcc
     1321 cacgcaacgc atcgttgccc ggctaaaccg tttgctgtct tcggggaagt gaaaatgcgt
     1381 ctccggttcc atcggatgat attgcgcgcc ggtgcgaagg ttccgcaact ggccatacgg
     1441 ttccgtgatc tttcccgggt tcatccgcca ttcggggtcc cagatctcct tgaattcgcg
     1501 aaacgcctgc acgagctccg gcccgtacat gatctccagc agatccgctt tggactggcc
     1561 gtccccgtgc tcgccggaga tgctgccgcc gaaactcacc acgaggcgcg cgccttcctc
     1621 cgtaaatcgt ttgtattggt ccaggccttc cggcgtcatc aggtcatacg gtaccctgca
     1681 gtggacgcag ccctgaccga aatggccgta gagcgggata ttccacccgt actttgcgaa
     1741 caggtcgcgc agcgcgacga ggtagtcgcc aaccctgtcc ggcggtaccg ccgtgtcctc
     1801 ccagccttct tcggtgatgg gctcaccggg cacccaggcg gtggccccaa gtcccgattc
     1861 ccggatcttc caaagcttgg tttcctgttc agggtcgtca aaaaggctca tcgccggcgg
     1921 gtcttttctg gctttcagct tagccatcat cgctttcccc aaagcgtccg attccgtttt
     1981 gctatcgccg ccgaattcca ccagcagcca ggctttgccg tcaggcagca gggtcaggtt
     2041 tttggtatga tagcccttct tttccatgtt atcgatcagc agatcatcga tcccttccaa
     2101 cgcgatgggc ttgaaagcga ggatttcggg aacgtgttta ccactgtcat acaggtccgg
     2161 gtagcccagg actaccaggc tcctggcttt gggattgggc atcagcttca acgtagcgga
     2221 aagtatggtc acacaggtgc tttctgtgcc gatcagggcg ctggcgacat tgaagccatt
     2281 ttccggcaaa agatccacca ggttatagcc ggaaacccgc cgggggatat tcggatatcg
     2341 tgtcctgatc tggtccgcgt accggtccac gaggtccttc agccgcgtgt agatctccgc
     2401 ccttcgtccc ccttgcgcaa cgatcgtccg gaactcctca tcggaggtgg ggccgacttc
     2461 c
//
LOCUS       DATGKE010000522         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1463650, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000522 DATGKE010000000
VERSION     DATGKE010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463650"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..>2333
                     /locus_tag="VKU83_08940"
     CDS             196..>2333
                     /locus_tag="VKU83_08940"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI93722.1"
                     /translation="MVVFAAASLHAQRWQQRVNYSIDVTLNDSTHSLDGSVRMLYINH
                     SPDTLPAIWIRCCANAFKSDYTAFSEALIREGRTDFYFSDKDRKGYINRLEFRADGQL
                     ARVEDHPRYIDVIRVILPHPLAPGDSVLLSTIYHVQLPDNFTGNGYHEGVYEVTQWYP
                     KPAVYDGSGWHPDPYLSQAVSYNEFGDVDVRIHVPAGFVVAAPVQADASMRYHTSGVP
                     DFVWFASRRFHHVHDTLRLPSGRVVGLYSFYSQPNSPVGRYGTGLLKDALRFYSNLLG
                     EYPFDQATVVETVETREVSEASRRPDAAAATYAGIVTADSRDTLYSDIKRSIARELAE
                     EWSAVLTGANGYRYPWMTEGMATYFYNRYRAGDDHLSPKRIPDMPDTFNRRAVNLRAV
                     LKTDQPISTSAEDFTSDNYRNIAGYKTSIWMADLRHAIGATAFDSCIRDYFRREAFHH
                     SGPPDFKAAFSAKSAPVDLDSAFRLLDTTGPVPPSPYHRRLQPNIQPIPHPTRSNSLN
                     IAPAVGFNNYEHWMIGVLIHNYNDIPDKLQLVATPLYSPVSHQWNGLLHLNRAWYPRK
                     AFREVEAGINGSRFSTLSGTDSSNHAIFGGSYKVVPYIRLFLPRAPLSTETIELEGKA
                     YLIGEKTFDNYLLKSTDSLYYPVSGKYRFRYLNQLTLSIRDERVLYPYAVNVQVQQSA
                     SFYRFNVDLHYFFNYAATGGLAVRLFGAKFG"
BASE COUNT          480 a          725 c          604 g          524 t
ORIGIN      
        1 gaacgcatga aatacgatct gatgtatatc gaaaacattt ccctcgtcct cgattggaag
       61 atcatgctgc ataccttacg gatcattatg tcggggcagg gaaggtaaac ggcgaaaggc
      121 ctgttgggcg ccggaagcgc cttgggctcc gggaggggcc gccatacgat gattaaacgc
      181 atatcctcca tactattggt tgttttcgcc gccgcctctc ttcatgccca acgctggcag
      241 caacgggtca actactccat cgacgtcacg ctgaacgact ccacgcattc cctcgacggc
      301 tcggtcagaa tgctgtacat caaccactcg cctgacaccc tccccgctat ctggatccgc
      361 tgctgcgcca acgccttcaa gagcgattat accgccttca gcgaggccct catccgggag
      421 ggccggacgg atttctactt ttctgacaag gaccggaaag gatatatcaa ccgcctggaa
      481 tttcgcgctg acggccagct ggcccgcgtg gaagatcacc cccggtacat cgatgtgatc
      541 agggttatcc ttcctcatcc cctggcgcct ggtgattcgg ttctgcttag cacgatctat
      601 catgtccagc tgcctgacaa tttcaccggc aacggttatc acgagggtgt ctacgaggtc
      661 acgcaatggt atccgaagcc cgccgtctat gacgggtcgg gctggcatcc cgatccatac
      721 ctgagccagg ccgtttccta taatgagttc ggcgatgtgg atgtccgcat ccatgttccg
      781 gctggttttg tcgtggctgc tccggttcag gcggacgcgt cgatgcggta ccatacatcc
      841 ggcgtcccgg acttcgtctg gttcgccagc cgccgattcc atcatgtgca cgatacctta
      901 cggcttcctt ccggccgggt cgtcggcctg tattcgtttt atagccaacc taattcgccc
      961 gtcggccgct acggtaccgg cttgctgaaa gatgcgcttc gattctactc caacctgctc
     1021 ggcgaatacc ctttcgacca ggctaccgtt gtcgaaacgg tcgaaacgcg ggaggtcagt
     1081 gaggcatcac ggcggccgga cgcggcggcc gcaacctatg caggcatcgt aaccgccgat
     1141 agccgggaca ccctttacag cgatattaaa aggagtatcg cgagggagct cgcggaggag
     1201 tggagcgctg tcctgacagg cgccaacgga taccggtatc cctggatgac ggagggaatg
     1261 gccacctatt tttataatcg atatcgggct ggggacgatc atctttcccc gaaacggata
     1321 ccggatatgc cggatacttt caatcgccgt gcggtcaacc tgcgggccgt cttgaaaacg
     1381 gaccagccca tctcgacctc ggccgaagat ttcacttccg ataactatcg gaacatcgcc
     1441 ggctataaaa cctccatctg gatggccgac ctccggcacg caatcggcgc cacggcgttc
     1501 gacagctgca tccgcgacta tttccggcga gaggcattcc atcatagcgg tccgccggac
     1561 ttcaaggctg ccttttcggc caaatcggcc cctgtcgacc tcgattcggc gttccggtta
     1621 ctcgatacga ccggtccggt cccaccctcg ccctatcacc ggcgcttaca gcccaatatt
     1681 caaccgatcc cgcaccctac gcggagcaac agtctcaaca ttgccccggc tgttgggttc
     1741 aacaattatg aacactggat gatcggggtc ctcatccaca attataacga tatcccagac
     1801 aagctgcaat tggtcgccac gcccctgtac tcgccggtca gccatcaatg gaacggcctg
     1861 ctgcatctca atcgcgcctg gtatccccgt aaggccttcc gggaggtcga agcaggtatt
     1921 aatggctccc gcttttccac gctcagcggg accgacagca gcaatcacgc gatctttggc
     1981 ggatcctata aggtcgtgcc ctatatccgg ctgtttcttc cgcgggcgcc tttaagcact
     2041 gaaacgattg aactggaagg aaaagcttac ctgatcggcg aaaaaacctt cgataactac
     2101 cttctgaagt ccacggactc gctgtattac ccggtcagcg gcaaataccg gttccgctat
     2161 ctcaaccagc ttaccttaag catccgggat gaacgggtgc tctatcctta tgcggtcaat
     2221 gtgcaggtcc agcagtcggc gtccttctat cgcttcaacg tcgatctgca ctatttcttc
     2281 aattatgccg ctaccggcgg cctcgcggtt cgccttttcg gcgccaaatt cgg
//
LOCUS       DATGKE010000523         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1074920, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000523 DATGKE010000000
VERSION     DATGKE010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074920"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VKU83_08945"
     CDS             complement(<1..1149)
                     /locus_tag="VKU83_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93723.1"
                     /translation="MILVLIAPALPACAQADTAADLDFYLGQAMQNSPLLKDYHNQVQ
                     LGQVDSQLIHASYLPQVNGNSANIYAPAYHGWGYDEAISNGGNFTTVVAVTKTLIGQK
                     HLDAQYETVRLNNQGIGNTAKISEQDLKRNVTAQYITTYGDLQQLNFNREIYALLEKE
                     SRLLKDLTERNVYRQTDYLAFLVTLKQEALQLRQLEIQFRNDHGTLNYLCGIVDTASG
                     LLAAPVIVLQALPDISNSVFFRQFHLDSLKLRNDRSLIDFSYRPKASLYADGGFSSSL
                     MYEPYKNFGVSFGVTVTMPIYDGHQRKLQYSKLDIEERTRTGYQQFFTSQYSQQIAQL
                     TQQLKSTEDLITDINEQVRYSQTLIDVNGKLLGTGDAKIADYILALSNY"
     gene            complement(1268..2542)
                     /locus_tag="VKU83_08950"
     CDS             complement(1268..2542)
                     /locus_tag="VKU83_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI93724.1"
                     /translation="MKLFSQYSRVNIVASISALLLGSIGYYFAVRFVLIHQLDDTIKI
                     EEAEILDNVHNHDRLPAPANYRDQQIEFIPAATTVPRTFYNTRKPSHHHHDMESYRAL
                     TFSVLVQKQLYTVIISKSEEESEDLLGLIMLTTAAMILLLLGTLFLANRLLLRRIWQP
                     FYRTLAGMREFNLSSRQPLAAAGASAIDEFRNLDDAARQMTRRILKDYEMLKGFSDNA
                     SHEMQTPLAIINSRLDLLIQDPVLEERHHRPIQAMYDAIGRLRQLNQSLLLLTKIENH
                     QFGRTDSVEIAPLIEEKLTQLEDPLKDRGLLVHKDLDRLRLPINGYLADILLNNLLLN
                     AIRHNKDGGDLSIRLRESSLRISNSGPALSFDPSTIFDRFVKGAHSGGTGLGLAIVRQ
                     ICDNYQFDLSYSYVDQRHTIDIRFPLQNFDKM"
     gene            complement(2570..>3206)
                     /locus_tag="VKU83_08955"
     CDS             complement(2570..>3206)
                     /locus_tag="VKU83_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLI93725.1"
                     /translation="VALDITDSIARARQEERERIGHELHDNVNQILTTANLYLRLPDE
                     EYFRTDDIRNKVSELTEAAIEEIRQLSRGLINPHFNDVGLVDHITRLVNDVRHAAPYT
                     IQFTHDEWSEIELLGKEQKLALFRILQEQIKNIIRHARATQVRISLHCVDDHVRLCIS
                     DDGQGFDAAQTPQGLGLSHIYERSRQCNGEISLETAPGKGCLLTVRMPLAV"
BASE COUNT          650 a          812 c         1006 g          738 t
ORIGIN      
        1 ataattgctg agggccagga tataatctgc gatcttggca tcgccggtgc ccagtagctt
       61 gccgttgacg tcgatgaggg tctgcgagta ccgcacctgc tcattgatat ccgtgatcag
      121 gtcttcggtg gacttcagct gctgggtcag ttgggcgatt tgctggctgt actggctcgt
      181 gaagaactgc tggtagccgg tacgggtgcg ttcctcgatg tcgagcttgc tatattgcaa
      241 tttgcgttga tggccatcat agatgggcat cgtgacggtt accccgaaac tcacgccgaa
      301 attcttataa ggttcgtaca taagagagga ggaaaaaccg ccgtcggcat agagactggc
      361 cttcggccga tagctaaaat cgatcaggga gcggtcgttc ctgagcttca ggctgtcgag
      421 atggaattgc cggaagaaca ccgaattcga aatatcgggc agggcctgca gcacgatgac
      481 cggggcggcc agcagccctg aggcggtatc tacgataccg caaagatagt tgagggtacc
      541 gtggtcgttg cggaattgga tctccagctg gcgcagttgg agggcctcct gtttcagcgt
      601 aacgagaaag gcaaggtagt ccgtttgcct gtagacgttc ctttcggtca ggtctttcag
      661 caagcgggat tccttctcga gaagcgcata aatctcgcga ttgaaattca gctgctgcaa
      721 gtcaccgtag gtagtgatat actgtgcggt gacattacgt ttcaggtcct gctccgagat
      781 cttggcggtg ttgccgatgc cctggttgtt cagcctgact gtctcatact gggcgtccag
      841 gtgcttctgg ccgataaggg tcttggttac cgccaccacc gtcgtgaaat tgccgccgtt
      901 actgatggcc tcgtcataac cccatccatg gtatgccggg gcgtagatat tggcgctgtt
      961 gccgttcacc tgaggaagat agctggcatg gatgagctgg ctgtcgacct ggcccagctg
     1021 gacctggttg tggtaatcct ttaacagtgg gctgttctgc atcgcctggc ccaggtaaaa
     1081 gtcaagatcg gcggcggtat ctgcctgtgc gcaagccggc agggccggag cgatcaaaac
     1141 caggatcatc gacagaactt gtcgggtcct tcggcaggtt gacattcttt gaaccatagg
     1201 cttatgtatc tgcgctggtg tctgttacgc cggcaggacc cggctttaac cggaagcaaa
     1261 cctagcctta cattttgtcg aaattctgta gcggaaaccg gatatcgatc gtgtgccgct
     1321 ggtcgacata agagtaggaa aggtcgaact ggtaattgtc gcagatctgg cggacaatgg
     1381 ccagcccaag gcccgtgccg ccggaatgcg cacccttgac gaaccggtcg aagatggtgg
     1441 acgggtcgaa tgacaacgcg gggccggaat tactgatgcg caggctgctt tcgcgaagcc
     1501 ggatcgaaag gtcgccgccg tccttattgt gccggatggc gttcaggaga aggttattga
     1561 ggagaatgtc ggcgagatag ccgttgatcg gcaggcgaag ccggtccagg tctttgtgga
     1621 cgagcagtcc gcggtctttg agcgggtcct cgagctgggt aagcttttct tcgatcagcg
     1681 gggcaatttc aacgctgtcg gtgcgaccga actggtggtt ctcgatcttc gtcagcagga
     1741 gcagggattg attgagctgc cgaaggcgcc cgatggcatc gtacatggcc tggatgggcc
     1801 ggtggtggcg ttcttccagc accggatcct ggatgagaag atccagcctg gaattgatga
     1861 tcgccagggg cgtctgcatt tcatgcgagg cgttgtccga aaatcccttg agcatttcat
     1921 aatccttcag gattcgccgt gtcatttggc gggccgcgtc gtccaggttg cggaattcat
     1981 cgatggccga ggcaccggcg gccgccaggg gctgacggct cgaaagattg aactcgcgca
     2041 tacccgcaag cgtccggtaa aagggttgcc agatccgccg aagcagcagc cggttcgcga
     2101 gaaagagggt gccgagcaac aggaggatca tggcagccgt ggtgagcatg atgagcccga
     2161 ggaggtcttc cgattcctcc tccgatttgc tgatgatgac ggtataaagc tgcttttgta
     2221 ccagcaccga aaacgtcagc gcgcggtagg attccatgtc gtgatggtga tggctgggct
     2281 tcctcgtgtt atagaaggtc ctgggaaccg ttgtcgctgc gggaataaat tcgatctgct
     2341 ggtcccggta gtttgccggc gccggcagcc tgtcgtggtt atgaacgtta tccaggatct
     2401 ccgcttcttc aatcttaatg gtatcgtcca gttgatggat gaggacgaag cgcacggcga
     2461 aataataccc aatgctccca aggagcaggg ccgaaatgct ggccacgata tttacgcggc
     2521 tgtattgcga gaagagcttc atgtccttag caaagctagg gaatcctcct cagaccgcga
     2581 ggggcatccg gaccgtcagg aggcagcctt tgcccggcgc cgtctcgaga ctgatctcgc
     2641 cgttgcattg cctggagcgc tcgtaaatat gcgagagacc caggccctgc ggcgtttggg
     2701 cggcgtcgaa accctggccg tcatcgctga tgcaaagccg gacatgatca tcgacacagt
     2761 ggagcgagat gcgaacctgg gtcgcccggg cgtgccggat gatgtttttg atctgctcct
     2821 ggaggatccg gaacagcgcc agcttctgtt ccttcccaag caactcgatc tcggaccatt
     2881 cgtcatgagt aaactggatc gtatacgggg cggcatgacg gacatcgttc acgaggcggg
     2941 tgatatgatc gaccaggcca acgtcgttga agtgagggtt gatcaggccg cgcgagagct
     3001 gacggatctc ctcgatggcg gcttcggtta gttcggatac cttattccgg atgtcgtccg
     3061 tccggaaata ttcctcgtcc ggaaggcgca ggtaaagatt ggctgtcgtg aggatctggt
     3121 tcacattgtc gtgcagctca tgaccgatcc gttcacgctc ttcctgcctc gcgcgcgcga
     3181 tggaatcggt gatgtcgagt gcgacg
//
LOCUS       DATGKE010000524         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1180857, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000524 DATGKE010000000
VERSION     DATGKE010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180857"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VKU83_08960"
     CDS             <1..193
                     /locus_tag="VKU83_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HLI93726.1"
                     /translation="YRISNFLLYQLAYAELYFTSVRWPDFRKQNLYEAILDYQGRERR
                     FGKTSAQIQQDLIAPGSTF"
     gene            337..>2629
                     /locus_tag="VKU83_08965"
     CDS             337..>2629
                     /locus_tag="VKU83_08965"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HLI93727.1"
                     /translation="MLRRSLFCVFLLTNYCLLTTNAAQAQATDSSKIRSADTSKPHPA
                     DSLHKALADTTKPGISIDPELVALASGKTPPKEYTIAGIKITGTKYLDESLLQSISGL
                     TVGDKVTIPGGDNFSKAINNLWRQNLFANVTIYFTKLVGTSIYIEINVTERPRLSNFY
                     FRGVRKGDGDDLGTKTGLIKGRVVTENMKRVAVQAIKKFYSDKGFQSTTVTISETRDP
                     AVQNSEILTFNIAKGPRVKIADVDFFGTHAVKESKLKKQMKDTKEVSRLTLFPPSNVT
                     PFGRRDSITLREYLRNWGFLSFTSTREFLDPYLRFKLFSSSKFNETKYEDDKQKVIDY
                     YNSKGYRDMTIVADAKPVDSLGKMHIAIKVDEGQRYYFGNISWKGNTKYPDSVLNLVL
                     GIHKGDVYNLDILNKRLGKQVSQEGGGISDLYMDDGYLFFHVEPVETAVYNDTIDFEI
                     RMTEGPQATIKNITIAGNDKTNEYVIRRELFTIPGDKFSRSAVISSTRAIANLGFFNQ
                     EKINPGINPNQDDGTVDINWTVEEKSGNQLELSAGWGGGIGLTGTVGVTFANFSVKNI
                     FKKEAWQPLPEGDGEKLSLRIQSNGKAYQSYNFSFTQPWLGGKKRNSLTLSLYRSVFR
                     TGGYNFRTSTYQFSDSNSLKNFGVTVALGKQLNWPDNFFTLTYSLNYTQYDLNNYPLF
                     TQAFRNGHSTNFSFKIALSRYNLDQPIYPRTGSSQMLSVQFTPPYSEIDRGLTQADSY
                     KVPEYHKWRFTSDWYLPIGNATGA"
BASE COUNT          693 a          698 c          680 g          558 t
ORIGIN      
        1 ataccggatc agcaatttct tattatacca actagcttat gccgaattat attttaccag
       61 cgtccgctgg cctgactttc gaaaacagaa tctttatgaa gccattctcg actatcaggg
      121 aagagagcgg agatttggta aaaccagcgc tcagattcaa caggatctga ttgctccggg
      181 gtctactttt taacaaagac ttttcataaa tccccttaat ttgccgacct gttccgccgg
      241 acctgaccgg aggttgcgaa ccagtccctg cctggggcag ggatgaggtt cattccgaag
      301 gacagaattt ggggctttca tttaaaaacc gactggatgc tacgaagatc gctgttttgt
      361 gtttttttat tgaccaacta ctgcttactg acgacaaatg ctgcacaagc ccaggcgacc
      421 gattcgagca aaatccgttc agccgatacc agcaaaccgc acccagccga ttcacttcat
      481 aaagccttag ccgacaccac aaaaccgggg atctccatcg atccggaact ggtcgcgctt
      541 gccagcggca agaccccgcc caaggaatat accatcgccg gcatcaagat cacgggcacc
      601 aagtatctgg acgaatccct gttgcaatcc atttcaggtc ttacagtcgg ggacaaggtc
      661 actatacccg gcggcgataa tttcagcaag gccatcaaca acctttggcg ccagaacctt
      721 ttcgccaacg tgaccatcta tttcaccaaa ctggtcggca ccagcattta catcgagatc
      781 aatgtgacgg aaaggccgcg gctttccaat ttttatttca ggggtgtaag aaagggcgac
      841 ggggacgacc ttggaaccaa gaccgggctg atcaaaggac gggtggtgac cgaaaatatg
      901 aaacgcgtgg cagtgcaggc catcaaaaaa ttttactcgg acaagggttt ccagagcacc
      961 accgtgacga tttcggagac aagggatcct gctgtccaga actctgaaat cctgaccttc
     1021 aacatcgcga aagggcccag ggtgaagatt gcggacgtcg atttcttcgg cacccatgcg
     1081 gtcaaggagt cgaagctgaa aaagcaaatg aaggacacga aagaagtgag ccggctgacg
     1141 ctgtttccac cgtcgaatgt gacgccgttc ggccggcgcg actcgattac gctccgcgag
     1201 tatctccgca actggggttt cctctctttc accagcacga gggaattcct ggatccttat
     1261 ctccggttca aattgttcag ctcgtccaag ttcaacgaga cgaaatatga agacgacaag
     1321 cagaaggtga tcgactatta caattcgaag gggtaccggg atatgacgat cgtcgccgac
     1381 gccaaaccgg tcgacagcct cggcaagatg cacatcgcca tcaaagtcga cgaaggccag
     1441 cgatattatt ttgggaatat ttcctggaaa ggcaacacga aatatccgga ctcggtcctg
     1501 aacctggtcc tggggatcca caagggggac gtctacaacc tggacatttt gaacaagcga
     1561 ttgggcaaac aggtcagcca ggaagggggc gggatcagcg atttatacat ggacgatgga
     1621 tatttgttct tccacgtcga accggttgaa accgccgtgt acaatgacac gatcgatttc
     1681 gagatccgga tgacggaagg accacaggct acgatcaaga acatcaccat cgcgggcaac
     1741 gacaagacca acgaatatgt gatccgtcgc gaactgttta cgatccccgg ggacaagttt
     1801 agccggtcgg ccgtgatctc ctcgacgcgg gcgatagcga atctgggatt cttcaaccag
     1861 gagaagatca acccgggcat caatcccaac caggacgatg ggacagtgga cattaactgg
     1921 acggtagagg agaagtccgg gaaccagctc gagctttcgg cgggctgggg cggcggcatc
     1981 ggtttgacgg gtacggtcgg ggttacgttt gcaaattttt cggttaagaa catctttaag
     2041 aaagaagcct ggcagccgct gcccgaagga gacggcgaga aactaagcct tcggatccag
     2101 tcgaacggca aggcctatca atcctacaac ttttcgttta cccagccctg gctgggcggt
     2161 aagaagcgca attcactcac gcttagcctt tatcgcagcg tattccggac cggaggatac
     2221 aatttccgaa ccagtaccta ccagttcagc gactcaaact cgctgaaaaa cttcggcgtg
     2281 acggtcgccc tgggcaagca gctgaactgg ccggacaact ttttcaccct gacctactcg
     2341 ctgaactaca ctcaatatga tttgaataac tatccgttgt tcacccaggc gttccggaac
     2401 gggcattcga ccaatttcag tttcaagatc gcccttagcc gctacaacct ggaccagccg
     2461 atatatccgc gaacgggttc cagtcaaatg ctgagtgtgc agttcacccc gccttattcg
     2521 gagatcgacc gggggctgac acaggcggac agttacaaag tgccggaata ccataaatgg
     2581 cgtttcacga gcgactggta cctgccgatc ggcaatgcga ccggggccg
//
LOCUS       DATGKE010000525         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_750531, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000525 DATGKE010000000
VERSION     DATGKE010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750531"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VKU83_08970"
     CDS             complement(<1..497)
                     /locus_tag="VKU83_08970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93728.1"
                     /translation="MDHKPTKRTAIVTGGGSGIGLAIAARFVKAGILTIIIGRDPQKL
                     TAAGEQLGEKCHAVTHDLSDLPSIPALVERITGRYGQIDILVNNAGTHLKKEFQDVTD
                     QEFDDVLVINLRSVFALSREVVKGMLPRRSGNIINISSMAARYGLPEVIAYTASKAGI
                     EGMTRA"
     gene            complement(500..>2359)
                     /locus_tag="VKU83_08975"
     CDS             complement(500..>2359)
                     /locus_tag="VKU83_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HLI93729.1"
                     /translation="LPALAALHLDEGLASLGDDGYLIRTVTITGSQHILIAAATDKGI
                     LYGVFRFLRLLQTQASLTRIDINSIPAYHYRILDHWDNLDRFVERGYAGISIWDWFRL
                     PGFKDPRYTDYARADASVGINGAVLNNVNASAVILRPEWLTKAAALADIFRPYGIRVY
                     LSVKWSSPIELGGLKTADPLDPAVRAWWAAKAAEIYRMIPDFGGFLVKASSEGQPGPQ
                     DYGRTHADGANMLADALAPYHGIVIWRAFVYRAANEDRAKQAYDEFQPLDGKFEDNVL
                     LQIKNGPVDFQPREPFSPLFGAMPDTHLLLEVELTQEYFGFSNHLVYLAPLFSECLNA
                     NTFRQGSGPIVAHTLSGIAGVANTGNDRNWCGHPFAAANWYAFGRLAWNPAASPREIA
                     VDWVRQTFTNDPGFVAPVLEMMLSSREIAVDYMTPLGLHHIMGYGHHYGPAPWYDKAP
                     RADWNPVYYHKADSAGVGFDRTPTGSNALQQYAAGARALWADASTCPDEWLLWFHHVP
                     WSWRMHTGRTLWQELCHKYYTGVDSVRRMQQRWDSLKGRIDDQRWSEVKMLLGIQEKD
                     AVWWRNACLLYFQTFSHLPLPAGSEVPDHTLAYYEKIQLYYAPGTPNNGVNFK"
BASE COUNT          508 a          654 c          691 g          506 t
ORIGIN      
        1 gctcttgtca tcccttcgat gccggccttc gacgccgtat aagcgatgac ctccggaagg
       61 ccgtatcggg ccgccatgga gctgatatta atgatattgc cgctacgacg gggaagcatt
      121 cccttcacga cctccctgga aagggcgaag accgatcgaa ggttgatgac cagaacgtcg
      181 tcgaactcct ggtcggtaac atcctggaat tccttcttca aatgggtgcc cgcgttattg
      241 acaaggatgt cgatctgccc ataacggccg gtgatccttt ctacaagcgc cgggatggaa
      301 gggagatcac tgagatcgtg cgtgaccgca tgacattttt cacccagttg ctcgccggcg
      361 gctgtcagtt tttgcggatc ccgcccgata atgatggtca agatccccgc tttgacgaac
      421 ctggctgcga tggccagacc aatgcccgac cctccgccgg taacgattgc tgttctcttc
      481 gtcggtttat ggtccatgct tatttaaaat ttaccccgtt atttggtgtt ccaggcgcat
      541 aatatagttg tattttttcg taataagcca gggtatgatc cggaacctca gacccggcgg
      601 gcaggggtaa gtgcgaaaac gtctgaaaat atagcaggca ggcgttgcgc caccagacgg
      661 catccttttc ctggataccg agcagcattt tcacttcaga ccacctttga tcgtcgatgc
      721 gccctttcag actatcccaa cgctgctgca tccgccggac ggaatcaaca cccgtgtaat
      781 atttatgaca gagttcctgc cacaaggtgc gacccgtatg catacgccag ctccagggaa
      841 cgtggtgaaa ccatagcagc cactcgtccg gacaggtgct ggcgtcggcc cacaatgcgc
      901 gggccccggc agcatattgt tgaagggcgt tggatcctgt cggcgtccgg tcgaatccga
      961 cgccggcgga atcggctttg tggtaataca ccgggttcca atcggcgcgc ggcgctttgt
     1021 cataccaggg cgccggaccg taatgatgac catagcccat aatgtgatgc agccctaacg
     1081 gcgtcatata gtccaccgca atctcccggg aagaaagcat catttccagt acgggggcga
     1141 cgaagcccgg atcattggta aaggtctgac ggacccagtc aactgcgatc tcccgcggcg
     1201 atgcggcggg attccaggcc aggcggccga atgcgtacca gttcgccgca gcgaacggat
     1261 ggccgcacca attgcgatcg ttgccggtat tggcaacccc ggcaattccc gaaagggtgt
     1321 gggctacgat cgggccgctg ccctgccgga aggtattggc gttcaggcac tcgctgaata
     1381 gtggggcaag ataaacgagg tggttggaga aaccgaaata ttcctgggtc aactctactt
     1441 cgaggagcag atgcgtgtcg ggcatggcgc cgaagagcgg tgaaaagggt tctcgcggtt
     1501 gaaagtcaac cggtccgttc tttatctgaa gcagcacatt atcttcaaat ttgccatcga
     1561 gcggctgaaa ttcatcgtag gcctgtttgg cccggtcctc gttggcggcg cggtagacga
     1621 acgcgcgcca gattacgatc ccgtgataag gcgctaatgc atcggcaagc atgttagcac
     1681 catcggcgtg agttcgcccg tagtcctgcg gtccgggctg cccttcggaa ctggctttta
     1741 ccaggaagcc gccgaagtcc gggatcatcc ggtagatctc cgccgccttt gccgcccacc
     1801 aggcccggac agcaggatcg agggggtcgg cggtttttaa gccgcccaat tcgatgggcg
     1861 aactccattt tacggaaagg tagacccgga tgccatacgg gcggaaaatg tccgccaggg
     1921 cggcagcctt tgtcagccat tccggtctaa ggatgacggc actggcgtta acgttattca
     1981 gcaccgcgcc gttgatccca accgacgcat ctgcgcgcgc ataatcggta tatctcgggt
     2041 ctttgaaccc ggggagcctg aaccagtccc agattgagat gcccgcgtag ccgcgctcga
     2101 cgaatcggtc cagattatcc cagtggtcca gtatccggta atgataagcc gggatactat
     2161 tgatgtcgat tcgggtaagg gaggcttgcg tctggagaag ccgcagaaaa cgaaagacgc
     2221 cgtagagaat gcctttatcc gtggccgcag caattaagat atgctgactg ccagtgatgg
     2281 ttactgtccg gataaggtag ccatcgtcgc caagtgaggc gagcccttcg tcgaggtgaa
     2341 gggcggcaag cgcgggcag
//
LOCUS       DATGKE010000526         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_562058, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000526 DATGKE010000000
VERSION     DATGKE010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562058"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1046)
                     /locus_tag="VKU83_08980"
     CDS             complement(54..1046)
                     /locus_tag="VKU83_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93730.1"
                     /translation="MTFLPFFPANGLFTLAEREFVPRALDIFRFQYAQNPLYRDFVRS
                     LGAGPESVGRIEDIPFLPVGFFKSYSVRTGEFLPEITFESSGTTGQTQSRHPVKDLDV
                     YKASYRSGFSAFYGPVQDWCVIGLLPAYLERSHSSLVFMVDDLIRLSAHPDSGFYLYD
                     HERLRDVLLRLEAEGQKTLLIGVSFALLDFAEHHQLELHHTIVMETGGMKGRRREVTR
                     EELHAVLRARLGVPGVHAEYGMTELLSQAYSSGGGLFRCPPWMKVLVRSEDDPLEVTA
                     AGNGLVNVIDLANIWSCAFLATDDIGRIHPDGRFEILGRADNSDVRGCSLMLGG"
     gene            1156..1608
                     /locus_tag="VKU83_08985"
     CDS             1156..1608
                     /locus_tag="VKU83_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93731.1"
                     /translation="MKPGSFLMRTLLLALIVTAVIVVSCNKNSSGKPTLKLESISSPV
                     QVNDSMRAHFKFTDGTAISNGYFWSIRHRLNLLQATDTSGSDTVSFQLPSFSANTGEI
                     YFSLPWQGYLNETATQNDTMVFKFFVQTADSSQTSDTVTSPQIIVLYQ"
     gene            1650..2486
                     /locus_tag="VKU83_08990"
     CDS             1650..2486
                     /locus_tag="VKU83_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93732.1"
                     /translation="MRKVVDYIVFSSLYVAICSVVMIWQTSRLLIGISPSGRLLGFVF
                     FATICSYNFHWYLTPRTARLSHRVRWTHHHKVLHFILYLTGAVGAIIYFLYLARYAVA
                     IGFAALLTFLYSAPKLPYRYSQYLRSIAIGKTFFLTFVWVYVTAILPVIIAGAPLNGA
                     FYWYVSGRFTLIYACCLIFDFRDREDDRAEGIVSMVTKLNERNIDWLFLISMICFAVS
                     CLALAFWHYPALDIILLLVPGAVLALLYPETKRNYSDYLYYVVLDGIVMLSGLLMLIF
                     RI"
     gene            2519..>2781
                     /locus_tag="VKU83_08995"
     CDS             2519..>2781
                     /locus_tag="VKU83_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93733.1"
                     /translation="MAKAISKSKKDPAPSLLTTKSWDFLHRYIDNASPVGFESSGQKI
                     WLDYLKPYVDTQFTDPYGTAVGVVNPNAPFKVVIEAHADEISW"
BASE COUNT          628 a          836 c          666 g          651 t
ORIGIN      
        1 atccggcatt cgtaaccggg tgatccggca ttcatgacgg atcccgccga tgcctagccg
       61 ccaagcatca aactgcagcc cctgacatcg ctgttgtctg cgcgccccag gatctcaaac
      121 ctcccgtccg ggtgaatccg cccgatgtca tccgtagcaa ggaacgcgca ggaccagata
      181 tttgccagat cgataacatt gaccaatcca ttcccggccg ccgttacctc gagcggatcg
      241 tcttcgctcc tcaccagcac cttcatccag ggcgggcaac ggaacaggcc cccgccgctg
      301 gaataggcct gggagagcaa ttcggtcatt ccatattcag cgtgcacacc cggaacaccc
      361 agcctcgcgc ggagaaccgc gtgcagttct tcgcgggtga cttcgcgccg gcgtcctttc
      421 attccaccgg tttccataac gatcgtgtga tgcagttcca gctgatggtg ttccgcaaaa
      481 tcaagcaaag caaaacttac tcctataagc aaggtttttt gaccttcggc ctccagacgc
      541 agcaatacgt cccgcaagcg ttcgtgatca tacaggtaga acccgctgtc cggatgagca
      601 ctgagccgga tcaggtcgtc gaccatgaac acaagggagg aatgcgaccg ctccaggtag
      661 gccggcaata aaccgatcac gcaccagtcc tggaccggac cgtagaaggc cgaaaatccc
      721 gaccggtaac tcgctttata gacgtccagg tccttgaccg gatggcggct ttgcgtttgc
      781 cctgtcgtgc cgctgctttc gaaggtgatc tcgggcagga attcacctgt ccggacagaa
      841 taggatttaa aaaaaccgac gggcaggaag gggatgtcct cgatccggcc gaccgactcg
      901 gggcccgctc ccaaagaccg cacaaaatca cggtagagcg ggttttgtgc atactgaaag
      961 cgaaaaatat ccagagcccg ggggacaaat tccctttcgg cgagcgtaaa gaggccattg
     1021 gcgggaaaga aaggaaggaa ggtcatcagc actcagatac caggccgata attgttaaaa
     1081 aagtcctatt cggcccctta aaggtatgtc cttttcgaca aaattaggta ggtttgcagg
     1141 caacaaaacc ctttcatgaa gcctggctct ttcctgatgc gcaccttatt gctggcattg
     1201 atcgtaaccg ctgttatcgt cgtaagctgc aacaagaaca gttccggtaa acccacgttg
     1261 aagctggagt ccatctcctc tcccgtgcag gttaacgatt ccatgagagc ccatttcaag
     1321 ttcaccgacg gaacggccat cagcaatgga tacttttggt cgatccggca caggctcaac
     1381 ctgctccagg caaccgacac atccggcagc gatacggtca gtttccagct tcccagtttt
     1441 tccgcaaata ccggcgagat ctatttttcc ctcccctggc agggctacct caacgaaacc
     1501 gccacgcaga atgacaccat ggtcttcaaa ttctttgtgc agaccgccga tagctcgcag
     1561 acaagcgaca ccgtcacatc cccgcagatc attgttcttt accaataacc agggccggcc
     1621 tcagggccga ataaccggaa aacatcgtgt tgagaaaagt tgttgattac attgtcttca
     1681 gtagtctcta cgtcgcgatc tgctcggtag tgatgatctg gcaaaccagt cgcctgctga
     1741 ttggcatttc cccttccggc cggcttctcg ggttcgtttt cttcgcgacc atctgcagct
     1801 acaattttca ctggtacctg actcccagga cggcgagact gtcacaccgc gttcgatgga
     1861 cccatcacca caaggtcctt catttcatcc tctatcttac cggcgcggtg ggcgccatca
     1921 tctacttcct ttaccttgcc cgctatgccg tcgcgatcgg ttttgccgcc cttttgacgt
     1981 ttctctattc ggcgccaaag ctaccctacc gctattccca atatctgcga tcgattgcca
     2041 tcggcaaaac attcttcctg acattcgtct gggtatatgt gaccgccatc ctcccggtca
     2101 tcatcgccgg cgcgcccttg aacggcgcgt tttattggta tgtatccggt cgttttaccc
     2161 tcatctatgc ctgctgcctc atcttcgact ttcgggaccg cgaagacgat cgcgcggagg
     2221 ggattgtcag tatggtgacc aagctcaatg agcggaatat cgactggctc ttccttatct
     2281 ccatgatttg ctttgcggtt tcctgtcttg ccctcgcatt ctggcattat ccggcgctgg
     2341 acatcatcct tctcctcgtc cccggggccg tgctggcctt gctgtatccg gagaccaaac
     2401 gcaattattc tgactatctg tattacgtcg tcctggatgg catcgtcatg ctgtcgggcc
     2461 tgctgatgct aatcttcagg atttagtacc tttacgccca aaaaaccgga ataaatcaat
     2521 ggcaaaagct atctcgaaat caaaaaagga ccccgcgccc tcgctactta ccaccaaatc
     2581 ctgggatttt ctccaccgat atattgataa cgcctcacct gtaggcttcg aaagcagcgg
     2641 gcagaaaata tggctcgact atctgaagcc ctacgtagat acccaattca ccgacccgta
     2701 tggaaccgct gtcggcgtcg tcaaccccaa tgccccgttt aaggtcgtca tcgaagctca
     2761 cgccgacgag atcagttggt t
//
LOCUS       DATGKE010000527         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_314372, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000527 DATGKE010000000
VERSION     DATGKE010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314372"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VKU83_09000"
     CDS             complement(<1..577)
                     /locus_tag="VKU83_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLI93734.1"
                     /translation="MTIEKIYEIYLRHPSIKTDTRQLQPGDLFFALKGPNFNGNLFAA
                     KALQLGAAYVILDETPEAATIPAEAGSRLVLVPDVLKTLQGLALHHRRHFRIPFLAIT
                     GSNGKTTTKELIHAVLSSKYKTYTTKGNLNNHIGIPLTILSVGRDAQLAVIEMGANHQ
                     KEIEGYCAYTLPTHGLITNIGKAHLEGFGGPE"
     gene            641..>2449
                     /locus_tag="VKU83_09005"
     CDS             641..>2449
                     /locus_tag="VKU83_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93735.1"
                     /translation="MFRNYWKIALRQLGKQKFYSFIKIGGFALGIATCLLVALYIRHE
                     LSYDRDYANGDRLYRVINDYVRDDGSVGRGVWQEPAFAKAMKAEFPEVEQSGRLMPAP
                     LFYGAGSNEVRPQGRTEETYEEGFTYCDQSVLDMFGTPMLYGERAHALERPWSIVISK
                     RKADKYFPGQNPVGKLLYLNGDNTNPLTIGGVMADPPSTSHLQYDFLISLTGHELWPG
                     EQNTWMAENYHTYALLRPGTDPVKLQRKLVELLNKYQLPQMLSRGQTDAASKIKRITY
                     ELQPVRNIHLYSVGLDDTLVHGDIHMVWLFGAVGMFILLLAVINFINLSTARSAGRAR
                     EVGLRKAVGSSREGLMGQFLTESLLLSGISFLLAMGLAWGFLPVFDRMTGTGLTIPWL
                     SAWWLPSIVAAAIGVGLLAGWYPAVYLSRFRPAEVLKSVVSLGSRRSALRSTLVVFQF
                     TTSVILIIATIVVFRQLRLILDKDLGYNKDQVLLIEGTGTVDTAKYPHLRFQAFKHDL
                     MSLSGVRSVSISDFLPVNGGKRNGNSFWKAGRSKLDPRIGAQSWWADPDYFKTMGIAM
                     VKGRAFSYDIASDSAAVVINESMAEKLGPGDPIGQVI"
BASE COUNT          481 a          650 c          766 g          552 t
ORIGIN      
        1 cctccgggcc gccaaagcct tcgagatgcg ccttgccgat gtttgtgatc agcccgtgtg
       61 tcggcagcgt atacgcgcaa taaccttcga tttctttctg gtgattggcg cccatttcta
      121 tcacggcgag ctgggcatcg cggccgaccg acaggatggt gagcgggatg ccgatatggt
      181 tgttgagatt gcccttggtc gtgtaggttt tatattttga agaaagcacg gcgtggatca
      241 gttccttggt ggtcgtcttt ccgttgctgc cggtgatcgc gagaaaggga atgcggaaat
      301 ggcgacggtg atggagcgcg aggccctgga gtgtcttcag gacgtcgggg acgaggacca
      361 ggcggctgcc tgcttcggcc gggatggttg cggcctcggg tgtttcgtcg aggatgacat
      421 aggcggcgcc cagttgcagg gctttggccg caaacaggtt gccgttgaaa ttagggccct
      481 tgagcgcaaa aaaaaggtcg cccggctgta gctggcgcgt gtccgttttg atggaagggt
      541 gccggaggta gatctcgtat atcttttcaa tggtcatggg ataaagatat gcaacaaaac
      601 cggcccgcga ctgtccttag gttacaccaa agccagcctc atgttccgca attactggaa
      661 gattgccctc cgtcaactgg gcaagcaaaa attctattcc ttcataaaga tcggggggtt
      721 tgcgctggga attgccacct gcctgctggt cgccctctac atccggcacg agttgagtta
      781 tgaccgcgac tatgcaaatg gggaccgcct ttaccgcgtg atcaacgact atgtccgtga
      841 cgatggctcc gttgggcgcg gcgtttggca ggagccggcg ttcgccaaag cgatgaaggc
      901 ggagtttcct gaagtcgaac aatcgggcag gcttatgcct gctccccttt tctacggcgc
      961 gggaagcaac gaagtgcggc cgcagggcag gactgaagaa acgtacgagg aagggtttac
     1021 ttactgcgac cagtcggtgc tggacatgtt cggaacccca atgctgtatg gcgaacgggc
     1081 ccatgcactc gagcggcctt ggtccatcgt aatctccaaa cggaaggccg acaaatactt
     1141 tccgggccag aatccggtag gaaaactgct ctatctgaat ggcgacaaca cgaacccgct
     1201 gaccattggc ggcgttatgg cagatccgcc atccacctcg caccttcaat acgatttcct
     1261 gatttccctg acaggccacg agctctggcc gggcgagcag aatacctgga tggcggagaa
     1321 ctaccacacg tatgccctgc tcaggccggg gacggacccg gtaaaattgc agcgtaagct
     1381 cgtggagctc ctgaataaat accaattgcc gcaaatgctt tcccgcggcc agacggatgc
     1441 tgcaagcaag atcaaacgca taacctacga gctccagccg gtccggaaca ttcatcttta
     1501 ctcagtgggc ctggacgata ccctggtgca cggcgatatc catatggttt ggcttttcgg
     1561 ggcggtgggg atgtttatcc tgctgctggc tgtcattaat ttcatcaacc tttcgaccgc
     1621 ccggagcgcg ggccgcgcca gggaggtggg gttaaggaag gcagtcgggt cctcgcggga
     1681 agggttgatg ggccagttcc tgacagagtc gctgttattg agcgggatct ctttcctgct
     1741 ggcgatgggg ctggcgtggg gctttttgcc cgtttttgat cggatgaccg gtaccggatt
     1801 gacgataccc tggttgtcgg cgtggtggct cccttctatt gttgcggcgg ccattggcgt
     1861 cggcctgctg gccggctggt atccggcggt ctacctatcc cggttccggc cggcggaggt
     1921 cttgaaatcc gtggtgagcc tgggttcgag gcgatccgca ttgcgaagca ccctggtcgt
     1981 tttccagttc acgacgtcgg tgatcctgat cattgcaacc atagtagtgt tccggcagtt
     2041 gcggctgatc cttgacaaag accttggata caacaaggac caggtgttgc tgatagaggg
     2101 aacgggcaca gtcgacacgg cgaagtaccc tcatctgcgg ttccaggctt tcaaacacga
     2161 tctgatgtcg ctttccggcg tccgtagcgt ttccatcagt gatttcctgc ctgtcaacgg
     2221 cggcaagcgg aacgggaact cattttggaa ggccggccgg tccaagctcg atccgcggat
     2281 cggggcgcaa tcgtggtggg cggaccccga ttatttcaag acaatgggca tcgcgatggt
     2341 caaggggcgc gcgttctcct atgatatcgc ctcggactcc gccgcagtgg tcatcaacga
     2401 atcgatggcg gagaagctgg gcccgggcga ccccattggc caggtgatc
//
LOCUS       DATGKE010000528         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_947778, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000528 DATGKE010000000
VERSION     DATGKE010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947778"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1273)
                     /locus_tag="VKU83_09010"
     CDS             complement(350..1273)
                     /locus_tag="VKU83_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLI93736.1"
                     /translation="MSVIVVGSMAYDAIETPFGKTDWIVGGAATYVAYAASNFTRPIN
                     QVSIVGYDFAKEEMTELEKRGVNLEGVKIVKDKKSFFWKGRYHLDMNTRDTLVTDLNV
                     LTDFNPVIPESYQGSEFVMLGNVMPSLQISVVEQLRKRPKLIAMDTMNFWMDNALDDL
                     NRLLTMVDVLMVNDSEARQLSGQYSLVKAARTILKMGPRYLVIKKGEHGALLFFEDKV
                     FVAPALPLEDVFDPTGAGDTFAGGFIGHLAHTKDISFENMKTAIIVGSAMASFCVEKF
                     GPTRLKEITRADIDTRISQFVDLVNFDIKLI"
     gene            1375..1965
                     /locus_tag="VKU83_09015"
     CDS             1375..1965
                     /locus_tag="VKU83_09015"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLI93737.1"
                     /translation="MAKIQSKKNSTKKEVIIEKASRLFREKGFGAASMRDLAEHVGVE
                     AASLYNHIQSKSEILQTICFKVANEFISNLDAVEASHQSTLKKMEAIIRLHIRMMLDQ
                     YEFVYIADHEWRHLPEPYLSNFLNQRRNYRKRLGDIVEAGIARGEMKPIESYTAVLVI
                     LSAISGIDSWQRSRKNTSAEALEANMVKFLIEGLKK"
     gene            1978..>2670
                     /locus_tag="VKU83_09020"
     CDS             1978..>2670
                     /locus_tag="VKU83_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLI93738.1"
                     /translation="MATHFRKLRIQDVRRETADCVSVAFDIPPGLRDEFRYHQGQNIT
                     LRLMMDGAEVRRSYSICSSPLEPELRIAVKKVQGGLFSTYVNDHLQKGQELEVLAPSG
                     KFYTELSPGNKKHYLAFAAGSGITPVISLLKTTLAVEPRSTFTLVYGNRNRLSIIFRE
                     ELEALKNRYMERLALHHILSREELDVPVYQGRIDVRKCEELSARLIDLKSVDEVYLCG
                     PEQMIFSLKDWLE"
BASE COUNT          630 a          699 c          721 g          620 t
ORIGIN      
        1 gccgacgact gcgactttca ttatagtttg tttatgttca taaaaacaac ccgggcgcct
       61 gtgaccaggg ccaaaggcca gctatttgcc gtaacaaaag agccttcccg gaggaaggct
      121 cttattatct tggaatacaa aacaatcaaa accttccctg tcgggggagc ttcttgatct
      181 gctttgtatt tttcattccg gttttcattt cgggggcaaa aataatcacg aaaacggtcc
      241 ccgcaaaatt tttgtgccag cgcggcggca acgcggcggc ggcaacacgg cggcggcaac
      301 gcggctccgg tcaatgcgcc ggcgtccggc cattgcgccg gcgtccggct tagatcaact
      361 taatatcaaa attcacgagg tcgacgaatt ggctgatccg cgtgtcgata tcggcccgcg
      421 ttatctcttt tagccgggtg ggtccgaatt tctcgacgca aaaagaggcc atggccgaac
      481 cgacgatgat cgccgttttc atattttcaa atgaaatatc cttcgtatga gcgagatggc
      541 cgatgaatcc gcccgcaaaa gtgtctccgg cgcctgtcgg atcaaaaacg tcctccagcg
      601 gcaaagcggg cgccacaaaa accttgtcct caaagaagag cagggcgcca tgttcccctt
      661 ttttgatgac caggtaacgc ggacccatct tcaggatggt ccgggctgcc ttaaccagcg
      721 aatactggcc gctgagctgc cttgcctcgc tgtcgttaac catgaggaca tcgaccatcg
      781 tcagcaaccg gttcaaatcg tcaagggcgt tgtccatcca aaagttcatg gtatccatgg
      841 caatcagctt cggccgcttc ctcagttgtt ccacgacgct gatctggagg ctgggcatca
      901 catttcccaa catcacaaac tcgctgccct ggtagctttc agggatcaca gggttgaaat
      961 ctgtcagtac gttgaggtca gtgaccagcg tgtcccgggt attcatgtcg agatggtacc
     1021 tgcctttcca gaaaaaggac ttcttgtcct ttacgatctt tacgccctcg aggttgacgc
     1081 cgcgtttctc cagctcggtc atctcttcct tcgcgaaatc atacccgacg atggagacct
     1141 ggttgatggg acgggtgaaa ttgctggcgg cgtaggcgac gtaggtcgct gccccgccaa
     1201 cgatccaatc ggtctttcca aagggtgtct cgatggcgtc ataggccatg gatccgacga
     1261 cgattactga catgagatgg tttttgggcc gcaacctacg taaaattatt tttcggcggg
     1321 ttttttgccg caattcttaa cttgtgccgc taacggtcgt tagtttatcg aagtatggct
     1381 aaaattcaaa gtaagaagaa cagcaccaaa aaggaggtga tcatcgaaaa agcctcccgc
     1441 cttttccgtg aaaaaggctt tggcgctgcg agcatgcgcg atcttgcgga acatgtcggc
     1501 gtggaagcag cgagtctcta taatcatatc cagtcgaaat cggagatcct gcagaccatt
     1561 tgcttcaagg tcgccaatga gtttatcagc aacctggatg cggttgaggc cagtcatcag
     1621 agcaccctca aaaagatgga ggccatcatc aggctgcata tccggatgat gctggaccag
     1681 tatgagttcg tctatatcgc cgatcatgaa tggcgtcatt tgccggaacc atatctttcc
     1741 aattttctta accagcggcg gaattatcgc aaacggctcg gcgatatcgt cgaagcgggg
     1801 atcgccaggg gtgaaatgaa gccgatcgaa agctataccg cggtgctcgt gatcctctcc
     1861 gccatcagcg ggatcgactc ctggcaaaga tcccggaaga acacgagcgc cgaagcgctg
     1921 gaagcgaata tggtcaaatt cctcatcgaa gggttgaaaa aataaacatt cgtacgcttg
     1981 gctactcatt tcagaaagct caggatccag gatgttcgcc gcgaaacggc agactgcgtc
     2041 tcggtagcgt ttgacatccc gcccggtctg cgcgacgaat tccgttatca ccagggacag
     2101 aatatcacgt tgcgattaat gatggacggc gcggaggtcc gccggtccta ttcgatctgc
     2161 agcagccccc tcgagccgga gttgcgtatt gccgtgaaga aagtacaggg tgggctgttc
     2221 tctacctatg tcaacgatca cctgcaaaaa ggacaggagc tggaggtgct ggccccgagc
     2281 ggaaaattct atacggagct gagccccgga aataaaaaac attatctggc ttttgccgcg
     2341 ggcagcggca tcacgcccgt catctcgctg ctgaagacca cgctggcggt agagccccgt
     2401 agcacgttta ccctggtgta tggcaaccgc aaccggctat cgatcatttt ccgggaagaa
     2461 ctggaggcat taaagaaccg gtatatggag cggctggcgc tgcatcatat cctgagccgg
     2521 gaggagttgg atgtacctgt ctaccaggga cggattgacg tccggaaatg cgaggagctt
     2581 tccgcgcggc tgatcgatct caagtcggtt gatgaggttt atctctgtgg gcccgaacag
     2641 atgatctttt cgcttaagga ctggctggag
//
LOCUS       DATGKE010000529         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_546411, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000529 DATGKE010000000
VERSION     DATGKE010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546411"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VKU83_09025"
     CDS             <1..501
                     /locus_tag="VKU83_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLI93739.1"
                     /translation="VYPDFFKVAVSSSGNHDNSIYNRWWSEKHHGVKEIVSDKGDTSF
                     VYSIEKNPDIAKNLKGHLMLSTGDIDDNVHPGNTIRLANALIKADKRFDLVLLPGQRH
                     GYGTMTEYFWWRMADYFSRWLIGDETVRPTNMEEMDNEQPQVGEKADRRTPDEGDGDE
                     DAGWDW"
     gene            complement(509..2317)
                     /locus_tag="VKU83_09030"
     CDS             complement(509..2317)
                     /locus_tag="VKU83_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93740.1"
                     /translation="MKKFSVILQYLSRYKGKLVLYMFFNILSILFGLISLGMLSPFLD
                     ILFTSGGPGSKGVTLHSNAIGGLKDVLMHVARSYDKPTLLIVICLLIVISTFLKNLFY
                     YWSSYISSPVRSATITFLRNDVYEKILALPIGYFTERRKGDLISRMTNDIYEIESSVV
                     STLEGLIKDPLTIIGYLAYMIYLSPTLSLFLLILLPLTGFLIGRISRILKRHSQDAAE
                     RLGESLSVLDETLSGIRIIKGFGAELMMKLKFVKINDSLFHIRNKMNWRRDLASPLTE
                     TLGVVILTIILYAGGMLVIKGHGFSGGDMMTYIAVFGLLINPAKTLSTSIFNIQRGAA
                     AMTRVEDLLNAPVTIQDDPHARKLDDFRDRIEFRNVSFSYEEAGILESINLVIEKGKT
                     VALVGSSGAGKSTLADLVPRFHDVSGGELLIDGVDIRNYSLRSVREQMSIVTQEPILF
                     NDSIAANIALGKPDATQEEIVRAAEIANAHHFIMKKENGYATNIGDRGSKLSGGERQR
                     LTIARAVLKNPPILILDEATSSLDTESERLVQDAINKMMAHRTSIVIAHRLSTIRHAD
                     EIVVLQKGRIVERGTHEQLMHARGFYRKLVEMQEVK"
     gene            2385..2888
                     /locus_tag="VKU83_09035"
     CDS             2385..2888
                     /locus_tag="VKU83_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HLI93741.1"
                     /translation="MQESKRQKQVGALLLEELSDIFQRLGLNMIDGGLVSLTGVKLTP
                     DLLEARVYLSLFHAKDNAAAMKTIGDRSWEIKKELAARVKHQLRRIPSLQYFHDDALE
                     HADHMEAVFRKLREERGGEGGTGGSAKNGGERDGKGSPESSGDRDAGRQNPGDGKHGP
                     DPEGGEK"
     gene            2885..4111
                     /locus_tag="VKU83_09040"
     CDS             2885..4111
                     /locus_tag="VKU83_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93742.1"
                     /translation="MNLLFAWRYFRAKKSTNAINIIAWVSMSAIVIGAAALIIVLSVF
                     NGFEDLVKSLYTSFYPDLKLTPATGKVLTLTPEQLNRLRAVNGVRVLSCVVEEQAVLM
                     NGNNPPNSPVLKGVDSNFIKVSGVANNIDRGSFDVGTADRPLAVLGSGVDYALGIDPE
                     KAVEPLDVYLFRKNAPEYPVDPEASYTHSAMATAGEFRIQEDFDDHYVITNLDYVKQM
                     MGLGPNEYGAVELAVDDPKQVAVIKQDILRVLGGGYKVQTRYEQNQSLYNVMGTEKWV
                     TYFLLTLILAVAAFNMVGALTMLVWDKQKDIHVLKALGASNRRIRRIFLSEGLLLALM
                     GGLTGILLAVLICVLQLKYKLIPLEGGSFLLDYYPVKMVPADFGLVFLTVLLVALLAS
                     WLPARKAAATPIELKS"
     gene            complement(4129..4962)
                     /locus_tag="VKU83_09045"
     CDS             complement(4129..4962)
                     /locus_tag="VKU83_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLI93743.1"
                     /translation="MADLKAIASQIRRDIVRMVHGAQSGHPGGSLGCADFFSALYFKV
                     LTHDPEFHMEGKNEDLFFLSNGHISPVFYSALARSGYFDVKELATFRKINSRLQGHPT
                     THEHLPGVRVASGSLGQGMSVAIGAALGKKLNNDKNIVYSLHGDGELDEGQNWEAIMF
                     AAAHKVDNLISTVDWNGQQIDGTTKAVMDLGNIHAKFEAFGWTTLEMNGNDMDEVLAT
                     LDKARTLTGKGKPIAIMMHTVMGKGVDFMENDHHWHGVAPNDEQLQKALAQLPETLGD
                     Y"
BASE COUNT         1163 a         1358 c         1450 g         1135 t
ORIGIN      
        1 gtgtatcccg attttttcaa ggttgccgtc tcttcctccg gcaaccacga taattcgatc
       61 tataaccggt ggtggagcga gaaacaccat ggggtgaagg agatcgtcag cgacaaaggg
      121 gatacgagtt ttgtttattc aatcgaaaag aacccggata tcgcgaagaa tctgaagggc
      181 cacctgatgc tgagcaccgg cgatatcgat gacaatgtgc acccgggcaa caccatccgc
      241 ctggcgaacg ctcttattaa ggccgataag cgctttgacc tcgtcctgct tcccggccag
      301 cgccacggct acggcaccat gaccgaatat ttctggtggc gcatggcgga ttatttctcc
      361 cgatggctga taggagatga aaccgtgcgg ccaacgaata tggaggagat ggataatgaa
      421 cagccgcagg taggagagaa ggcagacagg cggacgccgg atgagggcga cggcgatgaa
      481 gacgccggct gggattggtg agggtcgatc atttcacttc ctgcatctcg accagcttac
      541 ggtagaaacc ccgcgcatgc atcagctgtt catgggtccc gcgctcgacg atccggcctt
      601 tttgcaggac aacgatctca tcggcgtgcc ggatcgtgct gagccggtgt gcgatgacga
      661 tgctggtccg gtgcgccatc atcttattga tcgcgtcctg caccaggcgc tcgctttcgg
      721 tatccagcga ggaggtggct tcatcgagga tcaggatcgg cgggttcttg aggacggcgc
      781 gggcaatggt gagccgctgg cgctcgcccc cgcttaattt gctgccgcgg tccccgatat
      841 tggtagcata cccgttttcc ttcttcatga taaaatggtg cgcattggcg atctccgcgg
      901 cccgcacgat ctcttcctgc gtcgcgtccg gtttgcccag cgcaatattc gcggcgatac
      961 tgtcattgaa aaggatcggc tcctgcgtga cgatgctcat ctgctcgcgg acagagcgaa
     1021 gcgagtagtt ccggatatcc acgccatcga tcagcaattc accgccgctc acgtcatgaa
     1081 atcgcgggac caggtccgct aacgtcgact tgcccgcgcc cgaactgccc accagcgcga
     1141 ccgtcttccc tttctcgata accaggttga tcgattcgag gatgccggcc tcctcgtaag
     1201 aaaagctgac gttgcggaat tcgatccggt cccggaaatc atcgagcttt cgggcatgcg
     1261 ggtcgtcctg gatggtcacc ggcgcgttca gcaaatcttc cacccttgtc atagcggccg
     1321 caccccgctg gatattgaag atggaggtcg ataacgtctt ggcggggttg atcagcagcc
     1381 cgaacacggc gatatacgtc atcatatcac cgcctgaaaa accatggcct ttgatgacaa
     1441 gcatcccgcc tgcgtaaagg atgatcgtga ggatcaccac gccaagggtc tcggtcagcg
     1501 gactggcaag gtcccggcgc caattcatct tgttgcggat atgaaaaagg ctatcgttga
     1561 tctttacgaa tttcagcttc atcatcagct ctgccccgaa gcctttgatg atccggatgc
     1621 ccgacaaggt ttcatccagc accgacaggc tttcgcccag gcgctcggcg gcgtcctgtg
     1681 aatggcgttt caggatccgg ctgatgcggc cgatcaggaa acctgtcaat ggcaggagga
     1741 tcaggagaaa gagcgaaagg gtggggctga gatagatcat atatgccagg tacccgatga
     1801 tcgtgagggg atccttgatg aggccctcga gcgtggagac caccgaagac tcgatctcgt
     1861 aaatatcgtt tgtcatccgc gagatcaagt ctcctttccg ccgctcggtg aagtagccga
     1921 tcggcagggc cagtatcttc tcatacacgt cgttccgcag gaaggtgatc gtagcgctcc
     1981 ggacaggcga cgaaatatag gaagaccaat agtaaaaaag atttttgaga aaggtggaaa
     2041 tcacaatcag gaggcagatc acgatcagca gcgtcggctt gtcataggaa cgggcgacgt
     2101 gcatcagcac atccttgagg cccccgatcg cgttgctgtg gagcgtcacg ccctttgaac
     2161 cgggcccccc gctggtgaag agaatatcca ggaaagggct tagcatcccc agcgatatca
     2221 ggccaaacag gatcgacagg atattaaaga acatatacaa aaccaatttg cccttatacc
     2281 tggacaaata ttggaggatc accgagaatt tcttcatgcc gtttttcgcc gtagatttgg
     2341 ggggtaaagg tagtaggaat ccggggaagt gtaaagttaa tgatatgcaa gaaagcaaac
     2401 gacagaaaca agtgggggcc ttgctccttg aggaattgag cgatatcttc cagcggctcg
     2461 gcctgaatat gatcgacggc ggactggtgt cgctaacggg cgtcaagctg acacctgatc
     2521 tccttgaagc aagggtatat ttaagtctct tccatgcaaa ggacaacgcc gctgcgatga
     2581 agacgatagg ggaccgtagc tgggagatca agaaggagtt ggctgcccgc gtcaagcacc
     2641 agcttcgccg cattccgtca ctgcaatact ttcatgacga cgcgctcgag cacgccgacc
     2701 atatggaggc agttttccgc aagctccgcg aagagcgcgg cggcgagggc gggacaggag
     2761 gcagcgcgaa gaacggtgga gagcgcgatg gaaagggcag cccggagagc agtggtgatc
     2821 gcgacgccgg ccggcagaat cctggtgatg gcaagcatgg accggacccc gaaggaggtg
     2881 agaaatgaac cttttgttcg cctggcggta tttccgggca aagaagtcca cgaacgcgat
     2941 caatataatc gcctgggtga gcatgagcgc catagtgatc ggcgccgccg cgctgatcat
     3001 cgtgctcagc gtattcaatg gttttgaaga tctggtaaaa tcgctgtaca ccagtttcta
     3061 tcccgatctc aagcttacgc ccgctaccgg caaggttctc accctcacgc cggaacaact
     3121 caaccggctg cgcgccgtga acggcgtccg cgtactatcg tgcgtggtgg aagagcaggc
     3181 cgtgctgatg aacggcaaca acccgcccaa ttcgcctgtc ttaaaaggcg tcgacagcaa
     3241 tttcatcaag gtctcgggcg tggcaaacaa tatcgacaga ggcagctttg atgtgggcac
     3301 tgctgaccgg ccgctcgctg tcctcggatc cggcgtcgat tatgcattgg ggatagaccc
     3361 ggaaaaagct gtcgaaccgc tggatgtcta tctgttccgg aagaacgcac ccgagtaccc
     3421 ggtcgatccc gaggcttcgt acacgcattc agcgatggcc acggccgggg aatttcgcat
     3481 ccaggaggat tttgacgacc attatgtgat caccaacctg gattatgtaa agcaaatgat
     3541 ggggcttggt ccgaatgaat acggcgcggt ggagctggcc gttgacgatc ccaagcaggt
     3601 ggccgtcatc aaacaggaca tcctccgggt gctgggcggc ggctacaagg tccaaacccg
     3661 ctacgagcag aaccagagcc tgtataatgt catgggcaca gaaaaatggg ttacctattt
     3721 cctcctgacg ctgatcctcg ccgtcgccgc tttcaatatg gtgggcgccc ttacgatgct
     3781 ggtatgggac aaacaaaaag acatccatgt cctcaaagcc ctgggcgcca gtaaccgccg
     3841 gatccggcgg atattcctca gcgagggact ccttctggcc ctgatgggtg ggctgaccgg
     3901 aatcttgctt gccgtcctga tctgcgttct tcagttgaag tacaaactaa ttccgctgga
     3961 aggcgggtca ttcctgctgg attactatcc ggttaagatg gtacccgcgg attttgggct
     4021 ggtctttcta accgttttgc ttgtggcgct gctggcttcc tggcttcccg cccgaaaggc
     4081 tgcggcgact cccatcgagc tcaagagcta agggtttacc ctaggagatc agtagtctcc
     4141 aagcgtctcg ggtagctggg caagggcttt ctgaagctgt tcgtcgttcg gcgcgacacc
     4201 gtgccaatgg tggtcatttt ccataaaatc gactcccttg cccatgaccg tatgcatcat
     4261 gatggcgatc ggtttcccct tgccggtcag ggttcttgcc ttatccagcg tcgcgagcac
     4321 ttcgtccatg tcgttgccgt tcatctccag cgtggtccag ccgaaggctt cgaatttcgc
     4381 atggatattc cccaggtcca ttacggcttt tgtggtgcca tcgatctgct ggccgttcca
     4441 gtcaaccgtg gagatcaggt tgtccacctt gtgcgccgca gcaaacataa tggcctccca
     4501 gttctggcct tcatccagtt ccccgtcgcc gtgcagcgaa tacacaatat ttttatcgtt
     4561 attgagtttt ttacccaggg cggcgccaat agcgacgctc atgccctggc cgagcgaacc
     4621 gctggctaca cggacaccgg gcagatgttc atgcgtggta ggatgaccct ggagccttga
     4681 attgatcttg cggaacgtgg ccagctcttt gacgtcgaaa tacccggatc tcgcaagcgc
     4741 ggaataaaag acgggagaaa tatgaccgtt agaaagaaaa aaaagatctt cattctttcc
     4801 ttccatatgg aactccggat catgcgtgag gactttgaag tagagggcgg agaagaaatc
     4861 agcacagccg agcgagcctc ccgggtgacc actttgcgca ccgtggacca ttctgacgat
     4921 gtctcgcctg atttgggaag cgatagcctt taagtcagcc atatggatgt agattggttg
     4981 aggattgcaa atctatagat tttgtttttt aagaacgcct tatttaatag cattatccga
     5041 aaaagtattc taattttgtt caagtaccaa aataggactg atgtttgatg ctcttctttc
     5101 gatagc
//
LOCUS       DATGKE010000530         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1258917, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000530 DATGKE010000000
VERSION     DATGKE010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258917"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /gene="ispG"
                     /locus_tag="VKU83_09050"
     CDS             <1..1783
                     /gene="ispG"
                     /locus_tag="VKU83_09050"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HLI93744.1"
                     /translation="KEAENLLNIRNELRRRGYTTPLVADIHFTPNAAEIAARIVEKVR
                     INPGNYIDKKKFAFIEYTDAEYNAEIDRIRERFTPLVKICKEYGTAMRIGTNHGSLSD
                     RIMSRYGDTPVGMVESAMEFLRVARDESFHNIVLSMKSSNPQVMVQAYRLLVRTMQEE
                     FGVCYPLHLGVTEAGDGEDGRIKSAIGIGTLLEDGVGDTIRVSLTEDPEFEIPVCKDI
                     VDRYRRLGTEQGPPIPPMESIPYSPFEYRRRPTREIINAGGHHVPVVVADFSRLPVIR
                     RDDLEAIGYAYDASLDKWNIADAAADYIYTANKPIGFRLPGTLRVIINQDVWNNRGDA
                     GYLPLFTAAEYLESGPPAGMPHLVSIDSAAAENAMLPQNLTSEAIICLWSSHPDAMLS
                     MRTYFCRLMNAGIQNPVVLKVHSRDSAVDEQLIHFSAEAGALLLDGMGDGLWLQSEPG
                     RTADHRVSGRRYLRATNPYQFLNNTAFAILQATRTRISKTEYISCPSCGRTLFDLQET
                     TARIRAVTHHLKGVKIAIMGCIVNGPGEMADADFGYVGSGPGKVTLYKGKEIVKRGVD
                     SSIALEELIHLLKESEVWAEPAPVSAP"
     gene            1877..>2773
                     /locus_tag="VKU83_09055"
     CDS             1877..>2773
                     /locus_tag="VKU83_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HLI93745.1"
                     /translation="MRFQIRRRQDHALDLIEIEDTANGTVVSLLPGFGGMLHGFSIQA
                     SGGSSVQVIDSYKDLAELQAGLKSSYKGAKLSPWPCRIRDGKYRFDGKQYQFMRLFSD
                     GSAIHGLLADRTFGVLEEAAGDHSGGVTLEYNYHRDDPGYPFEYSCHVRYILHPDSVL
                     EVVTSVTNLDASAIPIADGWHPYFKLGGRIDDWELQFHSAAIVEFDRQLVPTGRLLQY
                     DRFETPRRIGETALDNCFSVKPDLVSAACEVRNPATGLKVSFLPDARYPYLQIYTPPH
                     RTSIAVENLSGTPDCFNNQMGLT"
BASE COUNT          628 a          768 c          812 g          565 t
ORIGIN      
        1 gaaggaagca gaaaacctgc tcaatatcag gaatgagctg cgccgccggg gctatacgac
       61 gccgctggta gccgatattc attttacgcc caacgcggcc gaaatagccg cgcggatcgt
      121 ggagaaagtg cggatcaacc ccggcaatta tatcgataaa aaaaagtttg cgttcataga
      181 atacacggat gcggaatata atgcggagat cgaccggatc cgggaacgct ttactccgct
      241 ggtgaagatc tgtaaggaat atggaacggc gatgcgcata gggaccaatc acggttcgct
      301 gagcgaccgg atcatgagcc gctatggcga cacgccggtg ggaatggtgg aaagcgcgat
      361 ggagttcctg cgcgttgcgc gggatgagag ctttcacaac atcgtgctga gcatgaaatc
      421 cagcaatccc caggtcatgg tgcaggccta ccggctgctc gtgcgaacga tgcaggaaga
      481 attcggcgtt tgttatcccc tgcacctggg cgtcaccgaa gcaggagacg gcgaagatgg
      541 caggatcaaa tccgcgatcg gcatcggaac cttgctggag gatggagtcg gggatacgat
      601 ccgggtgtcg ctgaccgaag atccggaatt cgagatcccg gtttgcaagg acatcgttga
      661 ccggtaccgg cgcctcggga cggaacaagg cccgccgatc ccgcccatgg aaagcatccc
      721 ctattcaccc ttcgaatacc ggcggcgccc gacccgcgag atcatcaacg cgggcggcca
      781 tcatgtcccc gtggtcgtgg cggatttcag tcgactgcct gtcatccggc gcgacgacct
      841 cgaagcgata ggatacgcct atgacgcctc gcttgacaaa tggaatattg ccgatgcagc
      901 agccgattat atctataccg ccaataaacc gatcggattc cggctgccgg gaaccctgcg
      961 tgtcatcatc aaccaggacg tttggaataa ccggggagat gccggttatc tgccgctttt
     1021 cacggcggcg gaatacctcg aatccgggcc tcccgcaggg atgccgcatc tggtatcgat
     1081 cgattcggcc gcggcagaaa acgcgatgct tccacaaaac ctgaccagcg aggcgatcat
     1141 ttgcctttgg tcttcccacc cggacgcgat gctgtccatg aggacttatt tctgccgcct
     1201 gatgaacgcg ggaattcaga acccggtcgt tctcaaagtg cacagccgcg acagcgcggt
     1261 cgacgagcag ttgatccatt tttcagcgga agcgggcgcc cttctcctgg acgggatggg
     1321 ggatgggctg tggctgcaga gcgaacccgg caggaccgcg gatcaccggg taagcggccg
     1381 caggtacctg cgtgcgacaa acccgtacca attcctgaac aatacggcgt ttgcgatcct
     1441 gcaagccacc cggacacgga tctccaagac ggagtatatc tcctgtccgt cctgtggacg
     1501 aacgcttttc gatctgcagg aaacaacggc caggatccgg gcggtgaccc accatctcaa
     1561 aggcgtgaag attgccatca tgggttgcat cgtgaatggc cccggtgaaa tggccgatgc
     1621 ggatttcggt tatgtgggga gcggccccgg caaggtcacg ctatacaagg gtaaggaaat
     1681 tgtaaaaagg ggcgtagaca gttcgatcgc ccttgaagaa ttgattcatt tacttaagga
     1741 aagcgaggtc tgggcggagc ccgcaccggt ttcagcaccg tgaaacaact ccggtaagtc
     1801 acgctgactc cgggcccgtg cagcgatgtt atgttgaggc ttttcgatac attgcagaaa
     1861 aatccccttt tcgcagatgc gttttcagat tcggcgccgg caggatcatg cccttgatct
     1921 cattgagata gaagacacgg ctaacgggac agtggtttcg ttgctgccag gattcggcgg
     1981 catgctccat ggtttctcga tccaggcctc aggcggttct tccgtgcagg tcattgacag
     2041 ctataaagac ctggcggaat tgcaggcagg actcaagtcg tcttataaag gcgccaaatt
     2101 atctccctgg ccatgccgga tccgggatgg caaataccgt ttcgacggaa agcaatacca
     2161 gttcatgcga ctgttcagcg acgggtcggc catccatggg ctgcttgccg acaggacttt
     2221 cggcgtcctg gaagaagcgg ccggcgatca ttccggcgga gtcacgctgg aatacaatta
     2281 ccatcgggat gatcccggat atccctttga atattcctgc catgtccggt atatccttca
     2341 tccggacagt gtgctcgagg tggtgacgtc agtgaccaat cttgacgctt cggcgattcc
     2401 gattgccgac ggctggcatc cctatttcaa gctggggggc aggatcgacg actgggagct
     2461 gcagttccat tccgctgcga tcgtggaatt cgatcggcaa ctcgttccta ccggcagact
     2521 gttgcaatat gaccggtttg aaacgccccg gcgcatcggt gaaaccgctc tcgacaattg
     2581 cttcagcgtg aagccagacc tggtcagcgc agcatgcgag gtgaggaacc ccgcaaccgg
     2641 tttaaaggtc tcgttcctgc cggacgcccg gtatccctat ctccagatct acactccgcc
     2701 acaccggacg agcattgctg tcgaaaacct gagcggcacg ccggattgct tcaataacca
     2761 aatgggactg aca
//
LOCUS       DATGKE010000531         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1131462, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000531 DATGKE010000000
VERSION     DATGKE010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131462"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /gene="rplJ"
                     /locus_tag="VKU83_09060"
     CDS             <1..575
                     /gene="rplJ"
                     /locus_tag="VKU83_09060"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HLI93746.1"
                     /translation="IIGSRSAGAYNKKVMNKDQKNEVIELLKDKFKEYNNFYVTNTES
                     LTVEQVGKLRRICFDKKVEMKVAKNTLIKKALEGLDSKRYSGVYQSLNGVTALLFSEN
                     PKEPALIISSFRRDGNMEKPVLKAAFIDGDVFEGDNQLKSLTTLKSKHDLIGEVIGLL
                     QSPIKNVMSGLNAGAKLAGLVKALEDRAAN"
     gene            735..1118
                     /gene="rplL"
                     /locus_tag="VKU83_09065"
     CDS             735..1118
                     /gene="rplL"
                     /locus_tag="VKU83_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLI93747.1"
                     /translation="MADVKVIAEQLVGLTVKEVNELAKILKEEYGIEPAAAAVAVAAG
                     PAAGGGGAAAAEEKTSFNVILKAAGPNKLNVVKVVKDLTGLGLKEAKDLVDGAPKPIK
                     EGISKADADALVAKLKETGAEVELQ"
     gene            1349..>4312
                     /gene="rpoB"
                     /locus_tag="VKU83_09070"
     CDS             1349..>4312
                     /gene="rpoB"
                     /locus_tag="VKU83_09070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLI93748.1"
                     /translation="MSSTKLQNRINFGKIKHLAETPDLLEVQIQSFKEFFQLETTPDK
                     RNIEGLFKVFKENFPITDTRNIFMLEFLDYFIDPPRYTIEECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGNIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSIH
                     PNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRSIGYETDKDILE
                     LFGMADEVKADKKALQKYIGKKLAARVLRTWVEDFVDEDTGEVVSIERNEIVLERDTI
                     LDEEAIEMVVEMDVKSVFIQKEDVGGDYAIIYNTLNKDTSNSELEAVQHIYRQLRGAD
                     APDDETARGIIDKLFFSDKRYDLGEVGRYKINRKLNLNQPLDQKTLTKEDMIEIIKYL
                     VRLTNAKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTPV
                     DLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEVR
                     DVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINEMGFIETPYRKVQEGKVDLKKLTY
                     LSAEEEDVAKIAQANIRLDEKGNFVEDKIVSRQTGDFPILDKNEVEFMDVAPNQIVGL
                     SASLIPFLEHDDANRALMGSNMQRQAVPLIRPEMPIVGTGLEAKAARDARIQIHSEGE
                     GVVEFVDANEIHVRYDRSEQQKLVSFEDDLKIYKLTKFIKTNQETCINLKPAVKKGQK
                     VKEGDFLTEGYAVQDGELALGRNLKVAFMPWKGYNFEDAIVISEKVVKEDLFTSVHIS
                     EYELEVRDTKLGEEELTPDIPNVSEEATKDLDENGIIRVGAQIKEGDILIGKITPKGE
                     SDPTPEEKLLRAIFGDKAGDAKDASLKAPSGTEGVVISKKLFQRAKKDKNAKVREKAA
                     LEKIEKVHEKNTSDLMDVLLSKLTTLLKDHVSAGVNNNFGEVQIGKGAKFTGKNLASI
                     DYLNVNPLGWTGDAKT"
BASE COUNT         1146 a         1053 c         1265 g          848 t
ORIGIN      
        1 gaatcatcgg atcccggtca gccggggcct acaacaaaaa agttatgaac aaagatcaaa
       61 agaacgaagt cattgagctg ttgaaagaca agttcaagga atataacaac ttctacgtca
      121 caaacacgga gtcgctgacc gttgaacagg tgggcaagct tcgcaggatc tgttttgaca
      181 agaaggtgga aatgaaggtt gccaagaaca cgctgatcaa aaaggccctg gaaggtctgg
      241 acagcaagcg ttatagcggc gtgtatcagt cccttaacgg ggtgacggcc ctgctctttt
      301 ccgaaaatcc caaggagccg gcgctgatca tcagctcttt ccggagggat gggaacatgg
      361 agaaacccgt cctcaaggcg gcgtttatcg atggcgacgt cttcgaaggc gacaaccagc
      421 tgaagtcgtt gaccacactc aagagcaagc atgacctgat cggcgaagtc atcgggctgc
      481 tccagtcacc gatcaaaaac gtcatgagcg gcctgaatgc cggcgcaaaa ctggccgggc
      541 tcgtgaaggc gctggaagac agggcggcaa attaaacaac cgcagccttg ccgcaggcag
      601 gctgcctgaa gataaaagcc cgggtccggg gcttgcgctg cgctctttgc gcacgccgga
      661 agaaccgacc aattttttta aacctccgcc ggagcaattc aaaaaggctg tgaaggcgga
      721 aaaaattaac acatatggca gacgtaaaag taattgctga acagttggta ggactgactg
      781 taaaggaagt aaacgaatta gctaagatcc tgaaggaaga gtacggcatc gagcctgcag
      841 ctgctgctgt agccgttgcg gctggtcctg ctgccggtgg cggcggtgct gctgctgctg
      901 aagagaagac ctctttcaac gtgatcctga aagccgccgg ccctaacaag ctgaacgtgg
      961 tgaaagtcgt gaaggatctg accggacttg gcctgaagga agcgaaggac ctggttgacg
     1021 gcgctcccaa gcccatcaag gaaggtatca gcaaggctga tgccgatgcc ctggttgcga
     1081 aactgaagga gaccggcgcc gaagtagagc ttcagtaaac ggaaagacct ggttttcccg
     1141 ggaacattag ataaaaaaga ggctccgctg cacatttcct gatggaatgc ggcgggtctc
     1201 tttttttgct gtcttggcaa ataaatgcta catttgcggt ccgtttccct gatgcctagg
     1261 gttgatatcg cctgtcgcag agtgtaactg attgataatc agttaatgat tcgaagctcg
     1321 atcaataaaa aaaccggttt taaacaatat gtcttcaaca aaattgcaaa acaggatcaa
     1381 cttcggtaaa ataaagcacc ttgccgaaac tcctgatctt ctggaagtgc agatccaatc
     1441 ttttaaagaa ttttttcagc tagagactac gccggacaaa cgaaacatcg agggtttgtt
     1501 caaagtgttc aaggagaatt tcccgatcac cgatacgcgg aacatcttca tgctggaatt
     1561 cctggattat ttcatcgacc cgccccgcta cacgatcgag gagtgtatgg agcgggggct
     1621 gacctatgcg gttccgttga aggccaagtt gcggttgagt tgtaacgacg aggaacacgt
     1681 ggactttcaa acgatcgtgc aggatgtctt tttgggaaat atcccctata tgacgcctcg
     1741 cgggaccttc gtgatcaacg gcgccgagcg cgtcgtggtg tcacagctgc accggtcgcc
     1801 gggggtgttc tttggccagt cgatccatcc gaacggaacg aagatctatt ccgcgcgggt
     1861 gatccctttc aagggcgcct ggatggaatt cgcaacggat atcaacaatg tgatgtatgc
     1921 gtacatcgac cggaagaaga aattcccggt gacgacgctg cttcgttcga tcggttatga
     1981 aacggacaag gatatcctcg agctcttcgg aatggccgac gaggtaaagg cggacaagaa
     2041 agcgttacaa aagtatatcg gcaagaagct ggcagcgcgg gtcctgcgta cctgggtgga
     2101 ggactttgtc gacgaggata ccggcgaggt ggtgagcatc gagcggaacg agatcgtcct
     2161 tgagcgcgac accatccttg acgaagaggc gatcgagatg gttgttgaaa tggacgtcaa
     2221 gagtgttttt atccagaaag aagacgtggg tggcgattat gccattatct acaataccct
     2281 gaacaaagac acctccaact ccgagctgga agcggtacag catatctacc ggcagttgcg
     2341 cggagccgat gctccggatg atgagaccgc tcgcgggatc atcgataaat tattcttcag
     2401 cgataagcgg tacgatctcg gtgaggtcgg ccggtacaag atcaaccgca agctgaacct
     2461 gaaccagccg ctcgatcaga aaacgctgac gaaggaggac atgatcgaga tcatcaagta
     2521 cctggtaagg ctgaccaatg ccaaggccga gatcgacgat atcgatcacc tgagcaaccg
     2581 ccgggtaagg acggtgggcg agcagctgta cgcgcaattc ggcgtagggc tggcgcgcat
     2641 ggcgcggacg atccgggagc ggatgaacgt ccgggacaac gaggtattta cgccggttga
     2701 cctgatcaat gccagaaccc tgtcatcggt cattaattcg ttctttggga cttcccaatt
     2761 gtcccagttc ctggaccaga cgaacccgct gagcgagatc acgcataagc gcaggatctc
     2821 ggcgttaggc cctggcgggt tgagccggga gcgtgcggga ttcgaagttc gcgacgtgca
     2881 ttattcccac tatggcaggc tttgtacgat cgagactccc gaaggaccga atatcggcct
     2941 gatctcgacg ttgtgcgtac atgccaagat caatgaaatg ggctttatcg agacgccata
     3001 ccgcaaggtc caggaaggaa aggtcgatct gaagaagctt acctacctga gtgcggaaga
     3061 ggaagacgtg gccaagatcg ctcaggcgaa catccggctc gatgagaagg gtaatttcgt
     3121 cgaagacaag atcgtttcgc gtcagacggg tgatttcccg atcctcgata agaatgaggt
     3181 agaatttatg gacgtcgcgc cgaaccagat cgtcggattg agcgcctcgc ttatcccgtt
     3241 cctggagcac gatgacgcca accgggcgct gatgggatcg aacatgcagc ggcaggcggt
     3301 cccgctgatc cgccccgaaa tgccaatcgt cggcacaggt ctggaggcca aggccgcccg
     3361 cgatgcccgc atccagatcc actccgaggg cgaaggcgtc gtcgagttcg tagacgccaa
     3421 cgagatccat gtccgttatg atcgcagtga gcagcaaaag ctggtgtcgt tcgaagacga
     3481 cctgaaaata tacaaactca ccaaattcat caagaccaac caggagactt gcatcaacct
     3541 gaaaccggcg gtaaagaaag gccagaaggt taaagaggga gacttcctca cggaaggtta
     3601 tgcggtgcag gatggcgagc tggcgctggg gcgcaacctg aaggtggcct tcatgccctg
     3661 gaagggatac aacttcgagg atgccatcgt gatcagcgag aaggtggtga aggaagacct
     3721 gtttacgtcc gtccatattt ccgaatatga attggaggta cgggacacca agctgggtga
     3781 ggaagagctt acgccagata ttcccaatgt ctccgaggag gctaccaagg acctcgacga
     3841 aaacgggatt atccgggtgg gcgcccagat caaggaaggc gatatcctga tcggcaagat
     3901 cacgccgaaa ggtgaaagcg accctacgcc ggaagagaag ctgctgcgcg ccatcttcgg
     3961 cgacaaggcg ggcgacgcca aggacgcttc cttaaaggcg ccgagcggaa cggaaggggt
     4021 ggtcatctcc aagaagctgt tccaacgcgc gaagaaggac aagaatgcca aggtccgcga
     4081 gaaggcagcc ctcgagaaga tcgaaaaagt gcatgagaag aatacttcgg acctgatgga
     4141 cgtattgttg agcaagctga ccaccttgct gaaggatcat gtttccgccg gcgtcaacaa
     4201 caatttcggc gaagtgcaga tcgggaaggg tgcgaagttc acggggaaga acctggccag
     4261 catcgactac ctgaatgtta acccgctggg ttggacgggc gacgcgaaaa cg
//
LOCUS       DATGKE010000532         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_151056, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000532 DATGKE010000000
VERSION     DATGKE010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151056"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..551)
                     /locus_tag="VKU83_09075"
     CDS             complement(78..551)
                     /locus_tag="VKU83_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HLI93749.1"
                     /translation="MASRSNFPVFVVHRHQSTHLHYDFRLELNGVLKSWAVPKGPSMN
                     PADKRLAVMVEDHPLEYAEFEGTIPKGSYGAGIVDIWDHGTWRPDNELEDPGKSLSNG
                     SLEFTLYGKKLKGKFILVSMKHGRTKNAWLLIKCHDQYAINKDYDSELLLSPQTY"
     gene            complement(714..1871)
                     /locus_tag="VKU83_09080"
     CDS             complement(714..1871)
                     /locus_tag="VKU83_09080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLI93750.1"
                     /translation="MNPKIVLLLALATYTLSCRSTRMSAASRASYKQPADSTWNVAFE
                     SKMETLRRRYHIPGLSAGIVYQGKLVWKKGFGYADVDRQVAPDEHTVYQIASLTKTFG
                     SIILLQQVEAGKISLDDPIQKYGIGLGGRWGSDPRIKIKHLLTHTAMGTTWNGFKPGY
                     SFRYNGDWYSQLGKAIEKASGQTFGELLVDHIIRPLHLQYTAPSLDDSVDFALTGYDR
                     ASFAARVAKPYDWQHGHLVPIRRFKYGFSPAAGIMSCVSDLALYSNAIDQGDFLDSAT
                     WKKVFTPFVSPRGKVFQYGLGWFVTYYKGVKLVWHTGWWTGYSALLVKVPEKALTFII
                     LANSQDLSRPFYHIAQPIRGFSFYKPFHKNLNNTLLASDFGKAFLKQVAGL"
     gene            complement(1926..3179)
                     /locus_tag="VKU83_09085"
     CDS             complement(1926..3179)
                     /locus_tag="VKU83_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93751.1"
                     /translation="MKKLRSIWLLTTLLCGAQALRAQALRAQGDSAGRVMRTQEDSAG
                     RVTRAQEDTAKKEHKFTVKGYLKYLELASFTGDATKLITDQLIHNRLNLRYQPDAYWN
                     LRLEVRNRIYYGQLVQSYPGFDSLVTSGGGTLDLSRNWVARNAVLANSTIDRASVEYS
                     RGKWDITAGRQRINWGINTVWTPNDIFNTFNYFDFDYEERPGSDAARVQYNFTGSSSL
                     ELAASPGKTPGKDIAAALLHLNRWSYDFQVFSGVYQQDITAGAGWAGNIGGAGFKGEF
                     SYFDPLRKNADTAAFVTSLSVDYAFRDGIYVLVSGLYNSQGGNSLINITQLQSATLSA
                     KNIFPFRYTAFAEASYSFSPILKSSFAGMYSPSGNSLILLPTLTYSIANNWTLDLVAQ
                     SFFSRQDGSYRGIGTSLYLRIKWGF"
     gene            complement(3176..3862)
                     /locus_tag="VKU83_09090"
     CDS             complement(3176..3862)
                     /locus_tag="VKU83_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI93752.1"
                     /translation="MSSIIQTIHLSKTYNPDTVPVPAVSDVSLQIGEGEFTALVGPSG
                     SGKTTLLNLIGGLDYPTGGEVQIDGQDITKMSENRLIDFRLHNIGFVFQAYNLIPVLT
                     AAENIEFVMLMLRKTRRERQQRVDELLQEVGLADKRDKRPTELSGGQQQRVAVARALA
                     SKPRFILADEPTANLDSHSAENLLEIMAKLNGEEKMTFVFSTHDQRVIDKAKRVVTLV
                     DGRIVSDTRA"
     gene            complement(3868..>4679)
                     /locus_tag="VKU83_09095"
     CDS             complement(3868..>4679)
                     /locus_tag="VKU83_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93753.1"
                     /translation="GRYLSGKPNEIIMGEKLVGKLGVRLHGKVVLTFQSVNGDLTAGS
                     FRIAGIFRSRNSGFDESTVFTGFNDLGQLLGTGAAVHEIAIVLKDDRLAGTMAKRIQL
                     QNPGLLVQTWSALSPEMALLNGIFDQYLYIVMAIILLALLFGIINTMLMAVLERQREF
                     GMLLAIGMNKPRVFLMILIESVLLACAGIPAGILLTIGAIEWLARKGIDLSAFSQGLM
                     QYGFSNRVYPVLEASMFLPVTIMTAITAVLSALYPAYKALSFKPAEAIRKI"
BASE COUNT         1019 a         1249 c         1305 g         1106 t
ORIGIN      
        1 ttctccactt ccttcgccaa cattggtttt atggcattgg tgacttttat tctgcttcca
       61 ttgctcatta ccttatttta ataggtttga ggtgagagga gcaattcgct gtcataatcc
      121 ttattgatgg catactgatc atgacacttt atgagcaacc aggcgttttt ggtccggcca
      181 tgcttcatgc tgaccaaaat aaatttgccc tttaactttt taccatatag cgtaaactca
      241 agagaaccat tgctgagaga tttcccgggg tcttccaatt cattgtctgg ccgccaggta
      301 ccgtggtccc agatgtcaac gatcccggct ccataacttc ctttcggtat cgttccctcg
      361 aattccgcat attccagggg gtggtcttcc accataaccg ctaatcgctt gtccgccggg
      421 ttcatggacg gccctttagg caccgcccag cttttcaaca ccccattcaa ctcaagccgg
      481 aaatcgtagt gcaaatgcgt ggattgatgc cggtgtacta caaaaacagg aaaattactt
      541 ctgctggcca tatcggataa tttttcaaag caagtgtatt acgtcaagga atcaaatgag
      601 aggggtcagg ttcctttttg agagatatca tccggttcgg cctcagccgt cgacttgtgc
      661 agtcacgata aagaaaaaac cgaaggttgt cgtaccgtag ccgtttacgt tggctatagt
      721 ccggcgacct gctttaggaa tgcttttcca aagtctgagg ccagcagggt attattcaaa
      781 tttttatgaa acggcttgta aaaactgaac ccgcggatag gctgggcgat gtggtaaaac
      841 ggccggctca ggtcctggga attcgcgagg atgatgaagg ttaaggcttt ctccggaacc
      901 ttgaccagca atgcagaata gcccgtccac cagccggtat gccagaccag ctttacccct
      961 ttataatagg tcacgaacca gcccaggccg tattggaaga ccttgcccct gggagataca
     1021 aaaggggtga agactttctt ccaggtggcg gagtccagga agtccccctg gtcaatcgcg
     1081 ttggaataca gggccagatc ggatacgcaa ctcatgatcc cggcggcagg gctaaaacca
     1141 tatttaaatc gccggatggg taccagatgc ccatgctgcc agtcataggg tttggctacc
     1201 ctggcagcaa aggaggccct gtcgtatccc gtcaaagcga agtccacact atcatcgaga
     1261 cttggagccg tgtattgcag gtgaaggggg cggataatat ggtctacgag caattccccg
     1321 aaggtttgcc ctgaggcctt ttcaatggcc ttaccgagct gactgtacca gtcgccgtta
     1381 tagcgaaagc tatagcccgg tttaaaacca ttccaggtgg tccccatcgc cgtatgggtc
     1441 aagaggtgtt tgattttgat cctggggtcg ctgccccagc gcccacccag tccgatgccg
     1501 tatttttgaa tcgggtcgtc caggctgatc tttccggcct ctacctgttg cagcaggatg
     1561 atggagccga aggtttttgt tagggaagca atctggtaca cggtatgctc gtcgggcgcc
     1621 acttgccgat ccacatcggc gtacccgaac ccctttttcc agaccagttt cccctggtag
     1681 acgatcccgg cggacaaccc ggggatatgg taacgccgtc tcagggtctc cattttcgat
     1741 tcgaacgcga cgttccaggt gctgtcggcc ggttgtttgt agctggcccg ggaagcagcg
     1801 gacatgcggg tgctgcggca ggaaagggta tacgtggcga gcgcaaggag aaggacgatt
     1861 ttgggattca tcgctgtggg gcctttcggt aaagctaccc gttttgaggg cgggaaccta
     1921 aagatctaaa agccccattt gattcgcaag tataggctcg taccgatgcc ccggtagctg
     1981 ccatcctgcc gggagaagaa agactgtgcc acaagatcca gtgtccagtt attggcgatc
     2041 gaataagtaa gggtggggag taaaatgagg gaattaccgg acggggaata cattccggca
     2101 aagcttgatt tcaggatcgg gctgaaagaa taggaggcct cggcaaaggc ggtgtaccgg
     2161 aacgggaaga tgttcttggc ggagagggtg gccgattgaa gctgggtgat gttgatgaga
     2221 ctgtttcctc cctggctgtt gtacaggccg gagaccaata cgtagatgcc gtcgcggaaa
     2281 gcgtaatcca ccgataggga agtcacaaaa gctgccgtat ccgcattttt ccggagcgga
     2341 tcgaaatacg aaaactctcc tttaaacccc gcgccgccga tattccctgc ccagccggcg
     2401 ccggcggtga tatcctgctg atagactccg gagaaaacct gaaaatcata gctccagcgg
     2461 tttaaatgga gcagggcggc tgcaatatcc ttgcccggcg tcttgccggg gctggccgcg
     2521 agctccaggg acgaggaccc ggtgaaattg tattgtaccc gtgctgcatc gctgcctggc
     2581 cgctcttcgt agtcaaaatc gaaataattg aaggtattga aaatgtcatt gggcgtccac
     2641 actgtattga tgccccaatt gatgcgctgt cttcccgccg taatatccca tttgccccgc
     2701 gaatattcca cgctggcacg gtcgatggta ctgttggcca gcaccgcgtt acgggcgacc
     2761 cagttcctgg acagatccaa tgttccgccg ccgctggtca ccagggaatc gaaacccgga
     2821 tagctttgca ccagctgtcc ataatagatt cggttgcgga cttcgagccg caggttccaa
     2881 taggcgtccg gctgatatcg cagattgagc cggttgtgga tcagttgatc ggtgatcagt
     2941 ttggtggcat cgccggtgaa actggccagt tccagatatt tcagataccc cttgaccgta
     3001 aacttatgct cctttttggc ggtgtcttcc tgtgcccgcg ttacgcggcc ggcggagtct
     3061 tcctgggtcc gcattactcg gccggcagag tctccctggg cccgcaaagc ctgggcccgc
     3121 aaagcctggg cgccgcatag cagggtcgta agcagccata tggaccgtag ttttttcatg
     3181 cgcgggtatc cgagacgatc cggccgtcca caagggtcac gacccgcttt gccttatcga
     3241 tcacccgctg gtcatgggtg gaaaacacaa aggtcatctt ttcttccccg ttcagctttg
     3301 ccatgatctc cagtagattc tccgccgagt gggagtcgag attggccgtt ggttcatcgg
     3361 ccaggatgaa acggggcttg gaggccaggg cgcgggccac cgccacccgc tgctgttgcc
     3421 cgccagacag ctctgtaggc cgcttgtcgc gtttgtcagc gaggcctact tcctgcagca
     3481 gctcgtctac ccgctgttgc ctttcccgcc tcgtctttct cagcatcagc atgacgaatt
     3541 cgatgttctc cgcagcagtg agcaccggga tcaggttgta ggcctgaaat acaaaaccga
     3601 tgttatgcag cctgaaatcg atgagcctgt tctcgctcat tttcgtaatg tcctgtccgt
     3661 cgatctgcac ttctcccccg gtgggataat cgaggccacc gataaggttc agcagggtgg
     3721 tcttgcccga cccgctgggt cccacaagcg ctgtgaactc gccttccccg atctgcaggc
     3781 tgacgtcgct gacggccggc acggggaccg tgtcggggtt gtacgttttg gaaagatgga
     3841 tcgtttgaat gatgctgctc atacatttta tattttccgg atagcttccg ctggcttaaa
     3901 ggaaagggcc ttataggccg ggtatagggc ggacagaacc gccgttatgg ccgtcatgat
     3961 ggtcacgggc aggaacatgg aagcttccag caccggatag accctgttgc tgaacccata
     4021 ctgcatgagg ccctgtgaga aggcggagag gtcaatgcct ttccgggcca gccattcaat
     4081 cgcgccaatg gtcagcagga tgcctgccgg aatcccggca caggccaaca ggacggactc
     4141 gatcaggatc atcaggaata cgcggggctt gttcatcccg atggccagga gcattccgaa
     4201 ttcccgttgt cgttcgagca ctgccatcag catcgtgttg atgatcccga aaagcagggc
     4261 gagcaggatg atagccatga caatgtacag gtattggtcg aagatgccat tgagcaaggc
     4321 catttccggg gaaagggccg accaggtctg taccagcagg ccggggtttt gtagctgtat
     4381 ccgtttggcc atcgtgccgg ccagcctgtc gtctttcagc acgatcgcga tctcgtgcac
     4441 cgcggctccg gtacccagca attgtccaag atcgttgaag cccgtgaaga cggtggattc
     4501 gtcaaagccg gaattccgtg aacggaatat ccccgcgatc cggaaggacc ctgccgtgag
     4561 atcaccgttg acggattgaa aggtcagcac aaccttgccg tgcaaccgga ccccgagctt
     4621 cccgactaat ttttcgccca tgatgatctc attgggcttg ccggagagat aacggccct
//
LOCUS       DATGKE010000533         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1257706, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000533 DATGKE010000000
VERSION     DATGKE010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257706"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VKU83_09100"
     CDS             <1..533
                     /locus_tag="VKU83_09100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93754.1"
                     /translation="NTQRGVEELVAAMAFLDPRKFHLIIIGGGDNWDRIQELVHDKEL
                     SDRITLIPKVPFEMLRSLTPRADLGLSLDKPNNINHIYGLPNKIFDYLFAGVPVLVSR
                     LVELEKIVRTYDVGDFIDNHLPEHIAVRIEAISRDPDRLAVWKRNTGRVRQECNWENE
                     GRIVVDIFEQITSKSS"
     gene            540..1691
                     /locus_tag="VKU83_09105"
     CDS             540..1691
                     /locus_tag="VKU83_09105"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI93755.1"
                     /translation="MKERRLHIVCLDVPYPVDYGGVFDLFHKIRALHTAGVKVQLHCF
                     EYGRGQQPILNDYCDQVYYYSRCEGHKAFSHRLPYIVCSRSNGKLLDRLAEDDDPILL
                     EGVHCSYLLNDARFSRRRIILRLHNVEYRYYRELYHSERSIFRKIYYLHESNLLRQYE
                     RRIAGLVKILAVSAQDCSQYRKEFGAPDIDTLPVFLPFEQVQSNEGTGCFCLYHGNLS
                     VPENEKAVTWLLKKVFSSVDLPFIVSGRNPSSGLTRLIGRYPHICLIPNPSESELQDL
                     IAKAQVNILPSFNSTGIKLKLLNALYNGRHCIVNRDMVRGTGLEQLCQIADSADEMRS
                     KVVEVYGRSLQPEEIGMRKEILAGLYNQEENLARLLQSIWSDEPQVVEK"
     gene            complement(1773..2462)
                     /locus_tag="VKU83_09110"
     CDS             complement(1773..2462)
                     /locus_tag="VKU83_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI93756.1"
                     /translation="MAEIIVELKHCNIFQGDSLILQDVNLVVEKGQFVYLVGKTGTGK
                     SSLLKTLYGDIPLREGEGQVAGFNLRDIDWKNVPYLRRTLGVVFQDFQLLTDRNVNDN
                     LKFVLRATGWKDERLMDEKIADVLDKVGLKSKGFKMPFEMSGGEQQRVDIARALLNSP
                     KLILADEPTGNLDPETSDEIMQLLFQISRDYGTSIVMATHDYRVISKFPARMVKTERG
                     IVIDNATVSLD"
     gene            complement(2580..>3477)
                     /locus_tag="VKU83_09115"
     CDS             complement(2580..>3477)
                     /locus_tag="VKU83_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI93757.1"
                     /translation="SFPMKSVPNALALRNRILQTFEDTITAGPAERQALLNFVIVGGG
                     PTGVELSGAMAEMKRYILPKDYPGTDFSRLTIYLLEGSPNTLNAMSEPSKVKSRQYLE
                     DLGVIVRTGTVVEDYDGETVTLKGGETIAARNVIWAAGVTGNKLEGIAGTSVLKGNRL
                     KTNRYHEVEGMDSVYAIGDISWMATPRYPNGQPQLANVAISQAKNLAGNFKRELKGRP
                     RVEFEYHNKGTMATVGKRKAVVDLPRLHFQGFFAWLTWMFVHLMLILSVKNRLFIFLD
                     WMMSYFANDSTLRLMLRPDTDS"
BASE COUNT          794 a          972 c          929 g          782 t
ORIGIN      
        1 tcaatacgca gcgcggggtc gaagaactcg tagccgccat ggctttcctt gatccccgga
       61 aattccacct gatcattatc ggcggcggcg ataactggga tcgcatccag gaactggtgc
      121 acgacaagga actgagtgac aggatcacac tcatccccaa ggtgcctttc gaaatgctgc
      181 ggtcgcttac gccgcgggcg gatctcggac tctccctgga caagccgaat aacatcaatc
      241 acatttatgg cctgccgaat aagatcttcg attacctttt tgccggcgtg cccgtgttgg
      301 taagccgtct ggtcgagctg gaaaagatcg tccgtaccta cgacgtaggc gacttcattg
      361 acaatcattt acccgaacac atcgcggtca ggatcgaggc catcagcagg gatcccgacc
      421 gcctggcggt gtggaaacga aataccggga gggtccggca ggagtgcaat tgggagaacg
      481 aagggcgtat cgtggtagat attttcgaac aaatcacatc aaaaagcagt taatagggtg
      541 tgaaagaaag gcgattacat attgtttgcc tggacgtacc ttaccccgta gactacggcg
      601 gcgtgttcga tctgttccat aaaatcaggg ccctccatac cgcgggagta aaagtgcagc
      661 tgcattgttt cgaatatgga cgcggtcagc agccgattct caacgattac tgcgaccagg
      721 tatattatta ctcgcgctgc gaaggccaca aggcgttttc ccatcgcctc ccctacatcg
      781 tttgctcacg gtcgaatggg aagctcctcg accggctggc agaggacgat gatccgatcc
      841 tgctcgaagg cgtgcactgt tcctacctgc ttaacgacgc ccgtttcagc cggcgcagga
      901 tcatcctgcg gctccacaat gtcgaatacc ggtactaccg cgagctctat cattcggaac
      961 gatccatttt ccggaagatc tactatctgc acgaaagcaa cttgctccgg caatatgaac
     1021 ggcggatcgc gggcctggta aagatcctgg ccgtttcggc gcaggactgc agccaataca
     1081 ggaaggagtt cggcgctccg gacatcgaca cgctgcccgt tttcctgcct ttcgaacagg
     1141 tacaaagcaa cgaaggcacc ggctgttttt gcctttatca tggcaacctt tccgtgcccg
     1201 agaacgaaaa agccgtcacc tggctgttga aaaaagtctt ttcttcggta gaccttccat
     1261 tcatcgtttc cggcaggaac ccttcttccg gactgacccg gctcatcggc cggtatccgc
     1321 atatctgtct tatccccaat ccttctgagt cggagttgca ggacctgatc gccaaggcgc
     1381 aggtcaacat ccttccatcg ttcaacagca caggcatcaa gctcaaattg ctgaatgcct
     1441 tgtacaacgg caggcattgt atcgtcaacc gcgacatggt tcggggcacc ggccttgagc
     1501 agctatgcca gattgctgac agcgcggatg agatgcggtc aaaggtcgtt gaggtttacg
     1561 gccgttccct tcaacccgaa gagatcggga tgagaaagga gatcctggcg ggactttata
     1621 accaggaaga gaatctcgcc cggcttttgc aatcgatctg gtccgacgaa ccgcaggtgg
     1681 tggaaaagta agcgtgaaag gaacgcgccg gggaccgccg gtttgcgacc ccgggggccg
     1741 atgcgggcgg gatactcaat ccaatgagcc agtcaatcca gtgagacagt tgcattgtca
     1801 atgacgatgc cgcgctctgt ctttaccatc cgcgccggga atttgctgat cacgcgatag
     1861 tcgtgcgtcg ccatgacgat ggaggtccca tagtcccggg agatttggaa gagaagctgc
     1921 atgatctcgt ctgaagtttc cgggtccaga ttaccggtcg gctcatctgc caggatcagt
     1981 ttgggcgaat tgagaagggc ccgggcgata tcaacgcgct gctgttcccc accgctcatt
     2041 tcgaaaggca ttttaaagcc tttcgatttc agcccgactt tatcgagcac atcagcgatc
     2101 ttctcatcca tgagccgctc gtccttccaa cccgtcgcgc gtagaacgaa ctttaaattg
     2161 tcgttgacat ttcgatcggt aagaagctga aagtcctgga atacgacgcc cagggtgcgg
     2221 cggaggtagg gaacgttctt ccagtcgatg tcacgcaaat taaagccggc cacctggccc
     2281 tcgccctccc gcaacggaat gtcaccgtag agcgttttca gcaggcttga tttgccggtg
     2341 cctgtcttcc caacaaggta aacgaattgc cctttttcaa cgacgaggtt gacgtcctgc
     2401 aggatcaggc tgtctccctg gaagatattg cagtgcttta gttctacgat gatctcggcc
     2461 atgataagta caataatcga ttggtatcaa caaaaatagg gaaaaaacgc gtgtgcctgg
     2521 cggaagagtc ggggttccag gcgtaggagc gggtgtgccc ggcttctcca ccgccgattc
     2581 tacgaatccg tatcgggccg caacatcagc ctcagcgtgg agtcgttggc aaaatagctc
     2641 atcatccagt caagaaagat gaaaagccgg ttcttcacgc ttaggataag catcagatga
     2701 acgaacatcc aggtcagcca ggcgaaaaaa ccctggaaat gcaatcgcgg caagtcgacc
     2761 acagcctttc gcttgcccac ggtcgccatc gtgcctttat tatgatactc gaattcgacg
     2821 cgcggcctcc ccttcagttc ccgcttaaaa ttaccggcaa ggtttttagc ctgagagatc
     2881 gcgacattcg ccaactgcgg ctggccgttc gggtaccggg gggtcgccat ccaggagata
     2941 tcgccgatgg catagacgct gtccatccct tctacctcgt gatagcggtt tgttttcagc
     3001 ctgtttcctt ttaggacact ggtccccgcg atgccctcca gcttgttgcc ggtcacgccg
     3061 gcagcccaga taacatttct tgcggcaatc gtttccccgc ccttaagcgt aaccgtctcc
     3121 ccgtcatagt cctcgacgac ggtgccggtg cgcacgatta ccccaaggtc ctccaggtat
     3181 tgccgggact tcacctttga cggttcactc atagcattga gggtgttcgg cgatccttcg
     3241 agaaggtaaa ttgtcagccg cgaaaaatcg gttcccggat aatccttcgg gaggatatat
     3301 cgcttcatct ctgccatggc gccggaaagc tcgacccccg tcgggccccc accgacaatc
     3361 acaaagttca gaagggcctg gcgttcggcg ggtcccgctg taatcgtatc ctcaaatgtc
     3421 tgaagaatgc ggttgcgcaa ggccaaggca ttgggcacgg acttcatcgg aaaagaa
//
LOCUS       DATGKE010000534         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1371163, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000534 DATGKE010000000
VERSION     DATGKE010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371163"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VKU83_09120"
     CDS             <1..1008
                     /locus_tag="VKU83_09120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93758.1"
                     /translation="DFLRPRFPRVKWIANSENLGFSRASNQGLALAKGEHILFLNPDT
                     VLPEDFARNCLAFYRQAADAPGGLGVRMIDGNGRFLKESRRGFPTPWVAFCRLSGLTS
                     LFPRSRRFSEYYLGYLPADRSHRAPVLSGACFWVSRKALDAAGPFDEEFFMYAEDIDL
                     SYRLERAGFCNYYFAGTTIIHFKGASTQKDLRYVRQFYSAMSRFRRKHFHRGMPGLFQ
                     AAFELAIRTRAAVSAAGRLFTGHVAAPALTGRRTWLTGDPASIGRLRPALMHSRRLVT
                     DPQEANEVIFCQGAAFSFKELISEWAKMNRGMAAAIYAEGAEAIVSSRHHDNRAEILK
                     L"
     gene            complement(1003..1695)
                     /locus_tag="VKU83_09125"
     CDS             complement(1003..1695)
                     /locus_tag="VKU83_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93759.1"
                     /translation="MNLKLSPRYVIAFLILLFLMQQIHDWAHVLAVRVTCHCWASRAF
                     DTWLICGSPSPGQHALIAISGPLINFVLLWLGWALLHPESTVEEDSVGVALVFAAQPL
                     DLLVAAFKGGGDLTDSLRWIQQHGPASNHHFVSRLGVVLMLLLVIPPLVRAFLRLPGY
                     KGRLIAFPLLFLLPGWLQRLWHRELTHWFITPDTSLLHAYILVGAWTGLLLIGCILTA
                     RWLRKFIWELSL"
     gene            1764..3290
                     /locus_tag="VKU83_09130"
     CDS             1764..3290
                     /locus_tag="VKU83_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLI93760.1"
                     /translation="MALIEIQLPKTLAKALRLPQKSPRRQQLKVLRKLLRKARFTEFG
                     QKYRFDEILLNRHPDKKFQELVPTYDYSSIYAQWWKQTLQGKADICWPGKIKYYALSS
                     GTSEAGSKYIPITNDMLRGNRIVMVKQLLSLRNYRDIPVKSLSKGWLMIGGTTSLQKG
                     SGYYAGDLSGITARKVPFWFQPFYKPGKKIARTIDWTRKLEEVVRLAPQWDIGFVVGV
                     PGWIQLCMEKIIERYKLNNIHEIWPNLAFFVHGGVSFEPYRIGFEKLLGKPLTYIETY
                     LASEGFIAYQDTQHSRAMRLVTNTHIFLEFVPFDNKNFDADGNLVKKPQAYMIDEVEE
                     GKDYALLLSTAAGAWRYLIGDTIRFTDKERCEIVITGRTKHFLSLVGEHLSVDNMNQA
                     IKLVSEELNLSIREFAVAGMPHGLFFAHQWWLSCNDPADESLLRKRIDQKLRELNDDY
                     AVERNSALKDIYVKVLPEQKFMDFMRLKGKIGGQHKFPRVLKGKMLEDWQRFLETGSI
                     "
     gene            3303..3944
                     /locus_tag="VKU83_09135"
     CDS             3303..3944
                     /locus_tag="VKU83_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HLI93761.1"
                     /translation="MMEAVLKGLALGLVLAMSVGPVIFTIIKQSLNNGHPGGFSFVAG
                     VWISDILLVTISNTLTALVSSLQEHSAAIAYGGGGFLIAMGIYFVFFKKTVVAGDALT
                     SEGRFRKRDMARIFASGFIINTLNPGVILFWLGNATALSVSHTLKQRIVIFAVCLLIN
                     MGADVGKVMAAGTLGRKLTVRTLSIINKIAGTILIGFGIALIWDVMVFHHLPK"
BASE COUNT          954 a         1150 c         1138 g          875 t
ORIGIN      
        1 gactttctcc ggccccgctt tccccgggta aaatggatcg ccaatagcga aaacctcggc
       61 ttttcccgcg ccagcaacca gggcctggct ttggcaaagg gcgagcacat cctcttcctg
      121 aaccccgata cggtccttcc ggaagacttt gcgcgcaatt gcctggcctt ttaccgccag
      181 gctgccgacg cgcctggcgg tctcggcgtg cgcatgatcg acggcaacgg ccggttcctc
      241 aaagaatcgc ggcgtggatt tcctacgccc tgggttgcct tttgccggct ctcgggcctg
      301 acgtcgcttt tcccccgctc ccggcggttt tccgaatact acctcggtta tctgccggcg
      361 gatcgcagcc accgcgcgcc ggtactttcc ggggcttgtt tttgggtcag ccgcaaggcc
      421 ctggatgccg ccggcccttt tgacgaggaa ttctttatgt atgcggaaga catcgatctg
      481 agctaccggc tggagagagc gggcttttgt aactactatt ttgccggcac taccatcatt
      541 catttcaagg gcgcaagcac ccaaaaagac ctgcggtacg tccgccagtt ctatagcgcg
      601 atgagccggt tccgccgcaa acacttccat cgcgggatgc caggcctttt tcaggccgca
      661 ttcgaattgg ccatcaggac acgggcggcc gtttcggccg ccggccggct tttcacgggg
      721 catgtcgcgg ccccggcact gaccggacgg cggacctggc tcaccggtga cccggcatcg
      781 atcggccggc tgcggcctgc cctgatgcac agccgcaggc tcgtaaccga tccgcaggag
      841 gcgaacgagg tcattttttg ccagggagcc gccttttcat tcaaggaact gatctcggaa
      901 tgggcgaaaa tgaaccgggg catggcggct gcgatctacg cggaaggcgc ggaagccatc
      961 gtcagcagcc ggcatcacga taaccgggcg gaaatcttaa agttataagg agagttccca
     1021 gatgaacttg cggagccacc gggcggtcag gatacaaccg atcagcaaca atcccgtcca
     1081 ggcccccaca agaatatacg cgtgcagcag gctcgtatcc ggcgtgatga accagtgcgt
     1141 aagttcccgg tgccacaggc gctgcagcca tccgggcagc aggaacagca atggaaaggc
     1201 gatcagcctt cccttgtagc ccggaagccg caggaatgcc cgcaccagcg gggggatcac
     1261 cagcaatagc atcagcacca cccctaaccg gctcacaaaa tggtgattcg atgccgggcc
     1321 atgctgctgg atccagcgca gactatccgt cagatcaccg cctcctttga acgccgcgac
     1381 caggaggtcc aggggctggg ccgcgaatac cagggcaaca ccaacagaat cctcttccac
     1441 cgtgctctcc gggtgcaata gcgcccagcc aagccacagc agaacgaaat tgatcagtgg
     1501 accggagatg gcgatcagcg cgtgttgccc cggggaaggg ctgccgcaga tcagccaagt
     1561 gtcgaacgcg cgggatgccc agcaatggca ggttacccta acggcaagca catgcgccca
     1621 gtcgtggatc tgctgcatca gaaataatag gatcagaaag gcgatgacat aacgcggtga
     1681 cagtttaagg ttcatgaaat aaatgtaata aaaatatcat ccaaaacatt aaattcgccg
     1741 cagaataaag accgggcttc tccatggcat tgattgaaat tcaattacct aaaacgctgg
     1801 ctaaagccct gcgactgccc cagaagtctc cccggcggca acagttgaag gtattgcgga
     1861 aactcctccg aaaagcccgg ttcactgaat tcggtcagaa gtatcgcttc gacgagatct
     1921 tactgaaccg ccaccccgac aagaagttcc aggaactcgt tccgacctac gattacagct
     1981 ctatctatgc ccagtggtgg aaacagacac tccagggtaa ggccgacatt tgctggccgg
     2041 ggaaaataaa atactacgca ctttcgtccg ggacatccga ggccggcagc aaatatattc
     2101 cgatcaccaa cgacatgttg cgcggtaacc ggatagtcat ggtgaaacaa cttctttccc
     2161 tgcgtaacta tcgcgatatt ccggtcaaaa gcctcagcaa gggctggctg atgatcggcg
     2221 gaacgaccag tttgcaaaaa ggatccggct attacgccgg ggacctcagc gggatcaccg
     2281 cccggaaggt ccctttctgg ttccagcctt tttataagcc ggggaaaaag atcgcccgca
     2341 cgattgactg gacgaggaaa ctggaggaag tggtccggct tgcgcctcaa tgggatatcg
     2401 gcttcgtcgt cggggtgcct ggctggatcc agctctgcat ggaaaagata atcgaacgct
     2461 ataagctgaa caacatccat gaaatctggc cgaatctcgc gttctttgtg catggaggcg
     2521 tttccttcga accctaccgc atcggcttcg agaaactcct cggcaagccg ctgacctata
     2581 tcgaaaccta tctggcgtcg gaagggttta tcgcgtacca ggatacccag cattcgcggg
     2641 ccatgcgcct ggtgaccaat acgcatatct tcctcgaatt cgtgcccttt gacaacaaga
     2701 acttcgatgc ggacggaaac ctggtcaaaa agccgcaagc ctatatgatc gatgaagtgg
     2761 aagaaggcaa ggactacgcc ctgctattga gtacggcagc gggcgcctgg cggtatttga
     2821 taggcgatac catccgcttc acggataagg agcgttgtga aatcgtcatt accggaagaa
     2881 ccaagcattt cctcagcctg gtcggtgaac acctgagtgt ggacaatatg aatcaggcca
     2941 tcaaactggt aagcgaggaa ttgaacctgt ccatccggga atttgccgtc gccggcatgc
     3001 cccatggcct gttctttgca catcaatggt ggttgtcctg taacgatccg gccgacgagt
     3061 ccctgttgcg gaagcggatc gatcagaagc tccgggagct caatgacgac tacgccgtcg
     3121 aaaggaacag tgcgttaaaa gacatttatg tgaaagtgct gccggagcaa aaatttatgg
     3181 acttcatgcg cctgaaagga aagatcggcg ggcagcataa gtttcccagg gtgctaaaag
     3241 gtaaaatgct cgaggactgg cagcgattcc ttgaaacagg aagtatctaa cctattacct
     3301 atatgatgga ggccgtattg aaaggattgg cgctgggact cgttcttgcc atgtcggtag
     3361 gccccgtcat ttttacgatc atcaagcaaa gcctgaacaa cggtcaccct ggaggattca
     3421 gttttgtggc cggcgtctgg atcagcgaca tcctgctggt caccatcagc aatacgctta
     3481 cggcactggt cagttccctg caggagcatt cggcggccat cgcttacggc ggtggcgggt
     3541 tcctgatcgc catgggcatc tatttcgtct ttttcaagaa gaccgtggtc gcaggcgatg
     3601 ctttgaccag tgaaggccgc ttccgcaagc gtgatatggc ccgcatcttc gcctcgggtt
     3661 ttattatcaa tacgctcaac cctggcgtta ttctcttctg gctgggaaac gctacggctt
     3721 tgtccgtaag ccataccctc aaacagcgga tcgtgatatt tgcggtttgc cttcttatca
     3781 acatgggcgc ggacgtcggt aaggtcatgg cggcgggtac gctcggccgg aagttgacag
     3841 tgcgcacgct ttccatcatc aacaagatcg ccggaacgat tttgatcggg ttcgggatcg
     3901 cactcatctg ggacgtgatg gtttttcacc acttgccgaa atagtgtccg cgacgttaac
     3961 aaccaacgtg gagctaaatt tgtatgtttg ataacatatg gcacgcaagc tctttatatt
     4021 aattatttac ctcattcctt tggtctgccg ggcgcagcac gacgtgctgg tgttgcaaaa
     4081 aaggggtatg cacgagcggg cctatacgat cggcgat
//
LOCUS       DATGKE010000535         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_800763, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000535 DATGKE010000000
VERSION     DATGKE010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800763"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VKU83_09140"
     CDS             <1..638
                     /locus_tag="VKU83_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93762.1"
                     /translation="EVKGHFLNVTDALDFLDRERVDLLFLDVEMPGANGFDLLDRLTY
                     SPMIILTTSKTEYAFNAFQYHVNDYLKKPFTYKRFLEAIGKLQLQYAEAGQNLPAGAT
                     AASPAESEFLFIKADDKLTKIRIDDILFLESMRDYVKFVTPSKNYVTYSTLKNMEEKL
                     SGATFLKVHRSYIVNLNKIDDIRGNTIYILGNQIPIGKGHKDGLEGRLNIL"
     gene            complement(1088..1549)
                     /locus_tag="VKU83_09145"
     CDS             complement(1088..1549)
                     /locus_tag="VKU83_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93763.1"
                     /translation="MQVIMALHSLLRWIILVLLIAAIVKSFGGMRSGRKFSGGDQKLG
                     LFLMISAHTTLLLGLILWFFGSLGLEPVREQGMGVVMKNPVLRYWVVEHFFGMLVAIA
                     LITIGRGVARKNIADTAKFRRSFWLFFLALVIILVTIPWPFRAGIGRPWTW"
     gene            1775..2785
                     /locus_tag="VKU83_09150"
     CDS             1775..2785
                     /locus_tag="VKU83_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLI93764.1"
                     /translation="MWKNLLVLFCWAAFCLAACKGNRDKQEEVREAILKQQPFAPLTD
                     SLDRQKGKDRSGLYFRRAELLSQNDQHELATEDYGKAWNLRPDPTTGVRYASSLTITG
                     QAGKAIRLLEDCRKRFPEDPAFAAMLADLFAQSGRFSDAIRVYDDILSTDSLNPDALY
                     ERGLLLEKNGDTASAIRSLKKAFGLQHLNTYGLELAHLYAEQGDESALPICDDILRKD
                     SARDMLDPFFIKGIYYSNTAQYKKAIVQFDSCVGRDWKFPDAYLEKGIALFKQKQYGK
                     ALESFQMTVKVSETYADGYFWIGRCYEATGRDQQALAYYRQALALDKTFSEAAERIKR
                     LQ"
     gene            2811..3461
                     /gene="rpe"
                     /locus_tag="VKU83_09155"
     CDS             2811..3461
                     /gene="rpe"
                     /locus_tag="VKU83_09155"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLI93765.1"
                     /translation="MVIVAPSLLASNFLRLEAVCQMLNESEADWLHLDVMDGRFVPNI
                     SFGIPVISQVRTATAKTCDVHLMIEEPERYAEAFRQAGANRLTVHLEACRHLHRNIQQ
                     IKSLGMAAGVALNPHSPVELLSDVLQDIDTVLIMSVNPGFGGQEFIPHTYQKIRSLKR
                     MIHERGLKTLIEIDGGVTIENAPNIIKAGADVLVAGSTVFHSQDPKGTIHTLKNSD"
     gene            3496..>4129
                     /locus_tag="VKU83_09160"
     CDS             3496..>4129
                     /locus_tag="VKU83_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI93766.1"
                     /translation="MKAVVAALLFLPMLIQPAFAQKKQAFVGGKVLDENDHPLPGVSV
                     TILGRQSGVTTSDSGGFRIRVDAGRAFALVFSFTGYRPQQRNFLLNDREEERVIIRLE
                     RGAALLRPAIVSDQQPSREAGLIKINPRDAINIPTPTGGIESLLKILVGSNNELTSQY
                     SVRGGNYDENLIYVNDFEVFRPYLVNNAQQEGLSFINPELTRTVSFYTGGF"
BASE COUNT         1029 a         1179 c         1031 g          890 t
ORIGIN      
        1 tcgaagtcaa aggacatttc cttaatgtaa cggatgccct tgatttcctc gacagggaac
       61 gtgtcgacct tctttttctc gatgtggaaa tgccgggtgc gaatggattc gatctgctgg
      121 accggctcac gtattcgccc atgatcatcc tcactacgtc caaaacggaa tatgctttta
      181 acgcgttcca atatcatgtg aacgactacc tgaaaaaacc tttcacctat aagcgattcc
      241 tcgaagcgat cggaaagctg cagctgcaat atgcggaggc cggacaaaac ctgccggccg
      301 gggcaaccgc agcgagcccg gcagaaagtg aattcctctt tatcaaagcg gatgacaagc
      361 tgaccaagat caggattgac gatatcctct tcctcgaaag catgcgcgat tatgtcaaat
      421 tcgttacgcc gtccaagaac tacgtcactt acagcacgct aaagaatatg gaagagaagc
      481 tgagcggtgc gacctttctg aaagttcacc gctcctatat cgtgaatctg aacaagatcg
      541 acgatatccg ggggaatacg atctacatcc ttggcaacca gatccccatc gggaaaggcc
      601 acaaggacgg actcgagggc cgcctgaaca tcctttgagg ggttttgatc cgttcgtcgg
      661 gcgcggttgt ccgtcgtcgg gcaaaagtat ccatttgtcg aaaaagtttc cattcaggat
      721 catcgttttg catctttgtt ccagcaaaca tcgatgtcct ttccgaagaa ccaaacgtcc
      781 aaatccgata ttcttattaa agacctgatc caagccagaa agaacccgtc ttaagtccaa
      841 aacctgaaga accgtcgtcc attcatcctt gaaaaccgcg tccaattatc cgaataaccg
      901 ggacatgcca gggacagacc gaagaaatga aatccgtcca aatgagtcca ttgtgatcga
      961 tccccaagcc tgaaactgaa agatcttggc cctgacaatg aattagccgc cacgcagtcc
     1021 gtggcggctt ccttatgtcc cgatcgcgtt tgtgatccac tatgcgccgg ccgcggcggg
     1081 cggcaactca ccacgtccag ggccggccga tcccggcgcg gaacggccac gggatggtga
     1141 cgaggatgat cacaagcgcc aaaaagaata accaaaagga ccgcctgaac ttcgccgtat
     1201 ccgctatatt cttcctggcc actccgcgcc cgatcgtgat caacgcgatt gcgaccagca
     1261 tcccgaaaaa atgttccacc acccaatagc ggaggaccgg attcttcatc accactccca
     1321 taccctgttc cctaacaggc tccagtccaa ggcttccgaa gaaccacagg atcagaccga
     1381 gcaataaggt cgtatgtgcg gatatcataa gaaaaagccc aagtttctga tcgccccctg
     1441 agaatttcct gccggagcgc atgccgccaa agcttttgac gatcgctgca atcagcagaa
     1501 caaggatgat ccaacgaagc aggctgtgga gggccatgat gacttgcata gcaaaataga
     1561 ttttacggtt ttctcaatcc ggccaaagct acaacaccgg aagcatgccc gcaagatggg
     1621 aaataattcc cgttccggga caggcaaagc gcaatcactg attattttta atttgatagt
     1681 cagccagttg cccggcggtc cgccccatcg gggacggcat tcttcaccct atcggctcga
     1741 caaagtttct tagatttgtc gaatgacggg tcaaatgtgg aaaaacctcc tggtgttgtt
     1801 ttgctgggca gcgttctgcc tggccgcctg caaaggcaac cgggacaaac aggaggaagt
     1861 gcgggaagcc attcttaaac agcagccctt cgccccactt accgatagcc tggatcgaca
     1921 aaagggtaag gacagatccg gcctgtattt ccggcgcgcg gaattactat cccaaaacga
     1981 tcagcacgaa ctggccaccg aagactatgg aaaggcctgg aaccttcggc ccgatccaac
     2041 caccggtgtc cgctatgcct cctcactcac gattacgggc caggcgggga aagcgatccg
     2101 gctcctcgag gactgccgca aaaggtttcc ggaagatccg gcattcgcag cgatgctagc
     2161 agatcttttt gcccagtcgg gtcgcttctc cgatgccatc cgcgtctatg acgacatcct
     2221 cagcaccgat tccctgaacc cggacgccct ctacgagcga ggcctcctcc tggagaaaaa
     2281 cggcgatacc gcttcggcca tccgatcgct gaaaaaagct ttcggccttc aacacctgaa
     2341 tacctatggc cttgaattgg cccatttgta cgcagagcag ggggacgagt ccgcgctgcc
     2401 gatttgcgac gatatcctgc ggaaagattc ggcccgcgat atgcttgatc ctttttttat
     2461 aaaggggatc tattactcga acactgcgca gtacaaaaaa gcaatcgtcc agtttgattc
     2521 ctgcgttggc cgcgattgga aatttccgga cgcatacctc gaaaagggca tcgccctctt
     2581 taaacagaag caatacggga aggcactcga gtcctttcag atgaccgtga aggtgtccga
     2641 gacttatgcg gatggttatt tctggatcgg ccggtgttat gaagcaacgg gcagggacca
     2701 acaggcgctc gcgtattacc ggcaagccct tgcattggac aaaacctttt cggaagccgc
     2761 cgaacgcatc aaacgattac aataacttta caccctaact ttatatcacc atggtcatcg
     2821 tcgccccgtc cttgctggca tcgaacttcc ttcgcctgga agcggtttgt caaatgctca
     2881 acgaaagtga ggcggactgg ttgcatctcg acgtgatgga cggccgcttt gtaccaaata
     2941 tcagtttcgg gattccggtc atcagccagg tgcggaccgc taccgccaag acctgcgacg
     3001 tgcatctgat gatagaagag cccgaaagat atgcggaggc gtttcgccag gcaggcgcca
     3061 accggctcac cgtccacctg gaagcatgcc gtcatctgca ccggaacatt cagcagatca
     3121 agtccctggg gatggccgcg ggcgtagcgc tcaacccgca ttctcccgtc gagctgttgt
     3181 cagacgtgtt gcaggatatc gataccgttc tgatcatgag cgtcaatccc ggcttcggtg
     3241 gccaggaatt catcccccac acctaccaga agatccggag cctgaaacgg atgatccatg
     3301 agcgcggatt aaagacgctg atcgagatcg atgggggcgt gacaatcgaa aatgcgccga
     3361 atatcatcaa ggccggcgcc gatgtgcttg ttgcgggcag tacggtattc cactcacaag
     3421 atccgaaagg gacgattcac acacttaaaa attcagattg aacgttccct atccgtcatt
     3481 gtaaaccatt cattcttgaa agccgtagtt gccgccctcc tttttctccc gatgctgatc
     3541 caaccggcct tcgcgcaaaa aaagcaggcc ttcgtaggcg gtaaggtcct ggatgagaac
     3601 gaccatccgc tgcctggggt gtccgtcacg attctcggcc gccagtcggg ggtcaccacg
     3661 tccgattcgg gaggctttcg gatccgggtg gacgccggcc gggcttttgc cctggtcttt
     3721 tcattcacgg gctaccggcc ccaacagcgc aacttcctgc tgaatgatag ggaagaggag
     3781 cgggtcatca ttcgcctgga aagaggcgcc gcattgcttc gccctgccat cgtctccgac
     3841 cagcagcctt cgagggaagc aggtttgatc aaaatcaacc ccagggacgc catcaatatt
     3901 ccaaccccta ccggcggcat cgaaagcctt ctcaagatcc ttgttggctc caacaacgaa
     3961 ctcacctccc agtactcggt ccgcggcggc aactacgatg aaaacctgat ttacgtgaac
     4021 gacttcgagg ttttccgtcc ataccttgtc aataatgccc agcaggaggg attaagtttc
     4081 atcaatcccg aacttacccg gaccgtcagc ttctatacgg gtggattcc
//
LOCUS       DATGKE010000536         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_685813, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000536 DATGKE010000000
VERSION     DATGKE010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685813"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1194
                     /gene="pheS"
                     /locus_tag="VKU83_09165"
     CDS             166..1194
                     /gene="pheS"
                     /locus_tag="VKU83_09165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLI93767.1"
                     /translation="MSSLLEQIAQYKQEIASAVPDGAEALESYRIRFLGTKGIVRNLF
                     AEMKNVPADNKREFGRVLNELKQLAESKYEQWKNTLSTAADETRPIDLSLPGNEVPLG
                     SHHPITLVKDRIVHIFGRLGFAVAEGPEIEDDWHNFTALNLPPDHPARDMQDTFYIHQ
                     NPDWLLRTHTSSVQARVMEKQRPPIRIICPGRVYRNETVSARANCFFHQTEGLYVDEQ
                     VSFADLKQTLYFFVQEMFGKDVRIRFRPSYFPFTEPSAEMDINCRICGGSGCSVCKHT
                     GWVEILGSGMVHPKMLDNFGIDSSRYTGFAFGMGIERICQLKYRVNDLRLYSQNDVRF
                     LRQFTGAV"
     gene            1212..2057
                     /locus_tag="VKU83_09170"
     CDS             1212..2057
                     /locus_tag="VKU83_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLI93768.1"
                     /translation="MIRIIGVCGAGTMGSGIAQVAAMAGYATILYDLDPPVLAKGRAA
                     IEKSLAALTERNKLSRAQQVASLGRLSFVHDRESCVADLIIEAIVEKPEPKLELLTRL
                     AAINSRETILTSNTSSLSLEALASKIPGPERFAGMHFFNPAPMMKLVEVVRTRSVSDA
                     TLQTILGVARQMGKTPVVCADSPGFIVNHVARPYYLEALRMVESGSTDVESVDLLMEA
                     TGFKMGPFRLMDLIGNDINYAVSESVYESLGRPERLKPSEMQRQKVQEGSLGRKTGKG
                     YYRYE"
     gene            2050..>2690
                     /locus_tag="VKU83_09175"
     CDS             2050..>2690
                     /locus_tag="VKU83_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLI93769.1"
                     /translation="MNKLQPTALILVTGGTGFLGAYIIRDLIQRGYSVRALHRHGSMP
                     FFLPAAIREQVEWVRCDIRDPLGLEDAMAGVDGVVHAAAKVSFSPRERRELWSINIEG
                     TANVVNTALDLKVRRFLFVSSVAAIGRTGGNEEVTEDRSWEHNRYNTNYAISKFRGEM
                     EVWRGIGEGLPAVIVNPSTILGYGDWNNSSCAIFRSVYHEFPWYTEGINGFVDV"
BASE COUNT          604 a          754 c          787 g          545 t
ORIGIN      
        1 ccgtttgccg atctgtccat ccttgtatcc ctggtcaccc gataattcat ccaggttaac
       61 cgggtccaga aagtcgctga tatgcaggtt ggacgacatc acaataggcg tttccggcaa
      121 acttaccaca aaatcgggga aactaaaatc acctatattt gtcgcatgag ttccctgtta
      181 gaacagatag cgcagtacaa gcaggagatc gcttccgctg ttcccgacgg cgcggaggcg
      241 ctggagtcat accgcatccg gttcctcggc acaaaaggaa ttgtcaggaa cctgtttgcg
      301 gaaatgaaga atgtgcccgc cgataataaa agggaatttg ggcgggtcct caacgaattg
      361 aaacagctgg cggagtcgaa atatgagcag tggaaaaata ccctttcaac tgccgcggat
      421 gagacccgcc cgatcgacct tagcctcccc gggaacgagg tcccgctggg cagccatcat
      481 cctatcacgc ttgtcaagga ccggatcgtc catatcttcg gccggctcgg gtttgctgta
      541 gccgaaggac cggagatcga ggacgactgg cataacttca ccgcgttgaa cctgccgccg
      601 gaccacccgg cccgtgacat gcaggacacc ttctatatcc accagaatcc cgactggctg
      661 ctgaggacgc ataccagcag cgtccaggcc cgcgtgatgg aaaagcaacg cccgccgatc
      721 cggatcatct gtccgggccg cgtctaccgg aacgaaacgg tcagcgcgcg ggcaaactgt
      781 ttttttcacc aaacggaggg attgtacgta gacgagcagg tatcgtttgc cgatcttaag
      841 cagacgctgt acttttttgt gcaggagatg ttcggcaaag acgtgcgcat ccgattcagg
      901 ccttcctatt ttccctttac ggaacccagc gctgaaatgg atatcaactg ccgcatttgc
      961 ggcggcagcg gctgcagcgt ttgcaaacac acgggatggg ttgagatcct gggcagcggg
     1021 atggtccatc ccaaaatgct cgacaatttc ggcatcgaca gcagccggta taccggattt
     1081 gccttcggga tgggcatcga aaggatctgt cagctgaaat accgtgttaa tgatctgcgg
     1141 ctgtattccc agaacgacgt tcgcttcctg cgtcaattca ccggagccgt gtaaaaccca
     1201 cttgtctgct tatgatcagg ataatcggcg tatgcggggc tggcaccatg ggcagcggga
     1261 tcgcccaggt tgctgccatg gcaggctatg caacgatcct ttatgacctg gatcccccgg
     1321 tgctcgcgaa gggaagggct gccattgaaa agagcctcgc cgctctcaca gaaaggaata
     1381 agctttcacg cgcgcagcaa gtggcgagcc tcggccggct gagcttcgtc catgaccggg
     1441 aaagttgcgt cgccgatctg atcatcgaag cgatcgttga aaaaccggag cccaaactcg
     1501 agctcctgac gcgactggcg gccataaaca gccgcgaaac gatcctgacc tcgaatacct
     1561 cgtcgctctc gctggaggcg cttgccagca agataccggg accggagcgg tttgccggaa
     1621 tgcacttctt taaccctgcg ccgatgatga agctggtgga agtggtccgg acccggtcgg
     1681 tgtccgacgc caccctgcaa accatcctcg gcgtggcccg gcaaatgggc aagacgcccg
     1741 tggtctgcgc ggattcgcct ggctttatcg tcaaccacgt ggcgcgtccc tattacctgg
     1801 aagcattgcg catggtggaa tccggaagta ccgacgtcga atcggtcgac ctcctgatgg
     1861 aagccaccgg cttcaagatg ggaccgttcc ggctgatgga cctgatcggg aacgatatca
     1921 attatgccgt cagtgaatcg gtgtacgaat cgctgggcag gccggaacgg cttaaaccct
     1981 cggaaatgca gcggcagaag gtccaggaag gcagcctcgg gagaaaaacg ggtaaaggct
     2041 attaccggta tgaataagct ccagccgaca gccctgatcc ttgttaccgg gggcaccggt
     2101 ttcctcggcg cttatatcat ccgcgacctg atccagcgcg gctattcggt gcgcgcgctc
     2161 catcgccatg gcagcatgcc gttctttctt ccggcagcga tccgggaaca ggtcgagtgg
     2221 gtccgctgtg acattcgcga tcccctgggc ctggaagatg ccatggcggg agtcgacggc
     2281 gtcgtccatg cggcagccaa ggtttcgttc tcgccgcggg aacggcgcga actgtggagc
     2341 atcaatatag aaggcacggc caatgtcgtc aacacggccc tcgacctgaa agtccggcga
     2401 tttttattcg tcagctcggt ggcggccatc ggcaggacgg gcggaaatga agaagtcacc
     2461 gaagaccgga gctgggagca taaccgctat aacaccaact atgccatcag caagttccgg
     2521 ggggagatgg aagtgtggcg ggggatcggc gagggccttc cagccgttat tgtcaatccg
     2581 agcacgatcc tgggatatgg cgactggaac aactcaagct gtgccatctt ccggagtgtt
     2641 taccatgaat ttccgtggta caccgaaggg attaacgggt ttgtcgacgt
//
LOCUS       DATGKE010000537         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1166528, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000537 DATGKE010000000
VERSION     DATGKE010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166528"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VKU83_09180"
     CDS             complement(<1..1321)
                     /locus_tag="VKU83_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93770.1"
                     /translation="MAIKKENRPRATFSKITIGLASPDSILERSYGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGMSSKKLESIIYYERYVVIQPGIRTDKGQNYGDLLT
                     EEEYLEILDTLPKDNQYLPDEDPAKFMAKMGAEAVHDLLERIELDQLSFDLRNAAANE
                     TSQQRKADALKRLSVVEAFRDANQRITNRPEWMVMQYIPVTPPELRPLVPLDGGRFAS
                     SDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAIDSLFDNSRKSNAVKAEG
                     GRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSARKLVDKKEAIVWDILENILKGHPVMLNRA"
     gene            complement(1637..>2849)
                     /locus_tag="VKU83_09185"
     CDS             complement(1637..>2849)
                     /locus_tag="VKU83_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93771.1"
                     /translation="LQSLKTLDKIKENARAHNQITFLLQILFFEKTIEALHITRSMQD
                     RADQLAQELDEVNDRLQLISRLSNLSLQLYGWFIRNGVARNEKDEEAVQEFFVRNMPA
                     DLSACPGFYEKLYQYQSYCWYAYIRQDFLMYYRYTQRWVDLFEKEPFMLAVETSQYIK
                     GMHNLLGANFDLLNDQKFRETLKQFEAFSHSDLVTSNVNNRIQTFVYLYISKLNQHFI
                     DGTFTEGLSLVPYIEEKLSEYWIYLDRHRILVFYYKIACLYFGSGDYEKTIDYLNKII
                     NWKMDLRTDLQCYSRLLHLIAHYELGNFEILEYLTKSVYRFMAKMESLSLVEEEIFKF
                     LRKAFTVPPDQLKPEFGILLERLKAYEKSRFETRAFMYLDVISWLESKIAGVPVQDVI
                     RGKYLSGRRRE"
BASE COUNT          619 a          824 c          688 g          718 t
ORIGIN      
        1 gggcccggtt cagcatcacc gggtgaccct tcaggatatt ttccaggata tcccaaacaa
       61 tagcttcttt cttatctacc agcttccgcg cgctcttcac cgttttcacg atgccccgct
      121 cgatcagctt ccgtattata aatggtttga acagctccgc agccatatcc ttgggcaaac
      181 cgcactcgtg cagcttcagc tcgggaccta ctacgattac cgaacggccg gaatagtcga
      241 cacgcttacc gagcaggttc tgccggaaac ggccctgttt ccccttgagc acatcgctta
      301 gcgatttcaa ggcccttccg ccctcggcct tgacagcatt ggacttccgc gagttgtcga
      361 acaggctgtc gatcgcctcc tgcaacatcc gcttttcatt acgtaggatc acctccggcg
      421 ctttgatctc cagcagccgc ttcaaacggt tgtttcggat gatcacccgg cgatagagat
      481 cgttcagatc ggaggaggca aaacggccgc catccagcgg aaccagcgga cgtaattccg
      541 gcggagttac agggatatac tgcatcacca tccactcggg acggttcgtg atacgctgat
      601 tggcatcgcg gaaagcttcc actacgctca gccgcttcaa cgcatcggcc ttacgctgct
      661 ggctcgtctc gttcgccgcg gcattccgca gatcgaagga aagctgatcc agctcgatcc
      721 gctccagcag atcgtgaacg gcttccgcac ccatcttggc catgaacttg gccggatcct
      781 catcgggtaa atactggttg tctttgggca gcgtatctaa tatctccaga tactcttcct
      841 ccgtaagcag gtctccataa ttctggccct tatccgtacg gataccaggc tgtatcacta
      901 cgtacctttc ataatagatg atgctctcca gcttcttgga gctcatcccc agcagatagc
      961 cgatcttatt gggaagcgat ttgaaatacc atatatgaac gaccggcact accagcttga
     1021 tgtgacccat ccgctcgcgc cggaccttct tttccgtaac ctcaaccccg caacggtcgc
     1081 acacaatacc cttgtagcgg atccgcttgt atttgccgca cgcgcactca tagtccttta
     1141 ccggaccgaa gattcgctca cagaacaagc cgtccctttc aggcttgtac gttcggtagt
     1201 tgatggtctc aggcttcaat acctctccat agctcctttc caggatgctg tcaggggaag
     1261 ctagaccgat agtgattttt gaaaaagtgg ctctgggacg attttctttt ttgatagcca
     1321 tattgcaata tgcggttttt gtcctgtcta atccattgca tatcaaaaga ttagcccgcc
     1381 cagcttacgc caggagagct acaattcaag cttttgtatg cttttgggac caaatcaagg
     1441 agggcaaagg tacgaatttt acccgaaaaa aactttttgt ccgggcccct atatccctcc
     1501 cggtttggcg acttaatgcg catgatgagc atttatgcgc gtcaccggtt ctaatctgcc
     1561 ccgggggagc ctcgccggta acctccgagg ccttataccg gggcctttcc ggcctccgga
     1621 tgtcctgccg cgggcactat tcccgcctcc ggccgctcag gtactttccg cggatcacgt
     1681 cctgcaccgg cacccccgca atcttgcttt ccagccatga tatgacatcc aggtacatga
     1741 atgcgcgggt ttcgaagcgg cttttctcgt aggccttcag ccgctccagc aggatcccaa
     1801 actcgggttt gagctggtca ggcggcaccg taaaggcctt ccgcaggaac ttgaagatct
     1861 cctcttccac caggctgagg ctttccatct ttgccataaa ccggtatacc gatttcgtga
     1921 ggtattcgag gatctcaaaa ttccccagct catagtgcgc tatcaggtgc agcagccggg
     1981 aatagcattg aagatccgtg cgcaggtcca tcttccagtt gatgatcttg ttgagataat
     2041 cgatggtctt ttcgtagtcg ccgctcccga aatacaggca ggcaatcttg taatagaaga
     2101 cgaggatccg gtgccggtcg agatagatcc aatactccga caacttctcc tcgatatacg
     2161 gaaccagcga caggccttcc gtgaacgtgc cgtcaatgaa gtgctggttc agtttggaga
     2221 tgtacagata gacgaaggtc tggatccggt tgttcacatt gctcgtgacc aggtcggaat
     2281 gagaaaacgc ttcaaactgc tttagtgtct cccggaactt ctggtcgttc agcaagtcga
     2341 agttcgcccc gagcaggtta tgcatgccct tgatatactg cgacgtttcg acagccagca
     2401 tgaacggttc cttttcgaag aggtccaccc aacgctgcgt atagcgatag tacatcaaaa
     2461 agtcttgccg gatatacgca taccagcagt agctctggta ctggtataat ttttcgtaga
     2521 agccggggca ggccgacagg tcggcaggca tgtttcggac aaaaaactcc tggacggctt
     2581 cctcgtcctt ttcattccgc gccacgccgt tccggataaa ccagccgtat agctgcagcg
     2641 aaaggttcga gagccggctg atcagctgga gccggtcgtt cacttcgtcc agctcctgcg
     2701 ccaactggtc ggccctgtcc tgcatgcttc gcgtgatatg caacgcttcg atcgtcttct
     2761 caaaaaacag tatctgtagc agaaacgtta tttgattgtg tgccctcgca ttctccttga
     2821 tcttatccag ggtcttcaga ctctgaaga
//
LOCUS       DATGKE010000538         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1203344, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000538 DATGKE010000000
VERSION     DATGKE010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203344"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2611
                     /locus_tag="VKU83_09190"
     CDS             <1..>2611
                     /locus_tag="VKU83_09190"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HLI93772.1"
                     /translation="PDRRQVFTILAAGAVGALAYTFCDSYWYSAVEGEVYASSAFFTA
                     LVFWAILKWEEHADRPGADRWLIFIFYMMGLSIGVHLLNLLTIPAIVMVYYFRRYRPT
                     LWGTIFAFLIGCALTGIVLKFVIQYTIKGAGAFDIFFVNDLHTPFFVGFGVFFTLLAF
                     LLILVNRWAVRKQYFYVKIACWSIGFMLLGYSTYFTTMIRSNADPAVDMYNVDNPVSL
                     EGYLSRDQYGDWPLLYGPDYTDPAPSAPGSDLYVKANGKYEVAGKSIVQDWSNTPSSH
                     FFPRMWNATNERQEINTYKRFGGVEDDDPPTMMNNLKYFWNYQTDWMFIRYFLWSYSG
                     KQNDLEGFGNVRDGNWITGIPFLDNPRLGDQSMMPDSIHINNKSYNRMYMLPLILGVL
                     GLVFQYNRHRKDFWIVMLLFFFTGWAIVIYLNQAGLQPRERDYAYAGCCYAFAIWIGL
                     GVVWAQELLAKYAFKNRIDASNYVAAGLCFLLVPVLMCGQEWNDHDRSKKTLARDLAK
                     DYLESCPRNAMLFSFGDNDTYPLWYAQEVEGIRPDVRVMVNTLLGTDWYINDLRYKIN
                     ESAPFDVIFTPQQIMGDRRNVAYFNDKMPGFDPNKYYDLYDILKNVVGSDDPKYTTTA
                     ENGDEINFLPTRKFSVPVDVNAARANGIIHPGDSVVSELHIDIPAGKRFLLKNDLAML
                     AVIAGSKWQRPICFTSPQDLNDLGLAKYVRLTGLSYELVPVEVNSGNRNLDAVDDDVA
                     YQNVMDKFAYGGASKPGVYYDEENRRHLNTIKFAHAQLAESLAQAGKKDSARKVLEHF
                     DQNVLQSNFPYGMTSNQNNLHDYFSYRFLEACYAAEDWSLAKKVDASLKKDLEQQLRY
                     YKSLGDNMPDDQLA"
BASE COUNT          566 a          759 c          696 g          590 t
ORIGIN      
        1 cccgacagac gccaggtctt cacgatcctg gctgccgggg ccgtcggggc gctggcgtat
       61 actttctgcg actcgtattg gtatagcgcg gtggaagggg aagtgtacgc ctcctcggct
      121 ttttttacgg cgctcgtttt ctgggccatc ctcaaatggg aagaacacgc cgaccggccg
      181 ggtgcggaca ggtggctcat tttcattttc tatatgatgg gcctttccat tggggtacat
      241 ctgctgaacc tgctgaccat tccggctatt gtcatggtat attatttccg gaggtaccgg
      301 ccgacgcttt gggggaccat attcgctttt ctgatcggct gcgcgctgac gggaatcgtg
      361 ctcaagtttg tgatccagta tacgatcaag ggcgcgggag cattcgacat cttttttgtc
      421 aacgacctgc atacgccttt ctttgtcggt ttcggcgtct tcttcacttt gctggccttt
      481 ttgctcatcc tggtcaaccg ctgggcggtc cgcaaacagt atttctacgt gaagatcgcc
      541 tgctggtcca tcggcttcat gcttctcggc tattccacct atttcacgac catgatccgg
      601 agcaacgccg atcctgccgt cgacatgtac aatgtggaca acccggtcag cctggaaggc
      661 tatcttagcc gcgaccagta cggcgactgg cccctgctct acggcccgga ttataccgat
      721 cccgcgcctt ccgcccccgg ctccgatctc tacgtcaagg ccaacggaaa gtatgaggtc
      781 gcgggcaaat ccatcgtcca ggattggagc aatactccct cgtcccattt ctttccgcgg
      841 atgtggaacg ccaccaacga acggcaggaa ataaatacct ataaaagatt tggcggcgtc
      901 gaagacgacg atccgcccac tatgatgaac aatctcaagt atttctggaa ttaccagact
      961 gactggatgt tcatccgtta tttcctgtgg tcctattcgg gcaagcagaa tgacctggaa
     1021 ggcttcggca acgttcgcga cggcaactgg atcaccggga ttccctttct cgacaatcca
     1081 cggctcggcg accagagcat gatgcccgac agcatccaca tcaataacaa gtcgtataac
     1141 cggatgtaca tgctgccact gatcctgggc gtcctcgggc tggtttttca atacaaccgg
     1201 caccgcaagg atttctggat cgtgatgctg ctgttcttct tcaccggctg ggccatagtg
     1261 atctatctca accaggcggg cttgcagccc cgtgaacggg attatgccta tgccggctgt
     1321 tgctacgcct ttgcgatctg gatcggcctt ggcgtggtct gggcgcagga actgctggca
     1381 aaatacgcct tcaagaaccg gatcgacgct tctaactacg tcgccgccgg gctttgcttc
     1441 ctgctcgttc ccgtcctgat gtgcggccag gaatggaatg accacgaccg gagcaaaaag
     1501 acgcttgccc gcgacctagc caaggattac ctggaaagct gtcccaggaa cgccatgctc
     1561 ttctccttcg gcgacaacga tacgtatccg ctctggtatg cgcaggaggt ggaaggcatc
     1621 cgccctgacg tccgcgtgat ggtgaatacc ctgcttggca ccgactggta catcaacgat
     1681 ctgcggtaca agatcaatga aagcgctccg ttcgacgtga tcttcactcc gcaacaaata
     1741 atgggcgacc gccggaacgt cgcctatttc aacgataaaa tgccgggttt cgatcccaat
     1801 aaatattatg acctctacga tatcctgaag aacgtggtcg gtagcgacga ccccaagtac
     1861 acgaccacgg ccgaaaacgg cgacgagatc aatttcctcc cgacgcggaa gttctcggtg
     1921 ccggtcgacg tgaatgccgc gcgggccaac ggcatcattc accccggcga tagtgtcgtc
     1981 agtgaattgc acatcgatat ccccgcgggc aaacggttcc tgcttaagaa cgacctcgcc
     2041 atgctggcgg tgatcgccgg cagcaaatgg cagcgcccga tctgtttcac gtcgccgcag
     2101 gatctgaacg atctgggcct ggccaagtat gtccggctga ctggcctctc ctatgagctc
     2161 gtcccggtgg aagttaattc cggtaatcgc aacctcgacg ccgtcgacga cgacgtcgct
     2221 tatcagaacg tcatggacaa attcgcttac ggcggcgctt cgaagccggg cgtatattat
     2281 gatgaagaga accggcggca cctcaacacg ataaagtttg cgcacgccca gctcgccgaa
     2341 agccttgccc aggcagggaa aaaggactcg gccaggaaag tgctggaaca cttcgatcag
     2401 aacgtgctgc aatccaattt cccctacggg atgacgtcga accagaacaa tctgcacgac
     2461 tatttctcgt accgcttcct cgaagcgtgc tatgcggcgg aggactggag cctggccaaa
     2521 aaagtggatg cctcgcttaa aaaagacctg gaacaacagc tgcggtatta taagtccctg
     2581 ggtgataaca tgcccgacga ccaactggcc g
//
LOCUS       DATGKE010000539         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1167744, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000539 DATGKE010000000
VERSION     DATGKE010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167744"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VKU83_09195"
     CDS             <1..1009
                     /locus_tag="VKU83_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HLI93773.1"
                     /translation="DCHDFTISFPNNSTPDYHGYNWLRTFSWTLGDGDTSTAVYPVHT
                     YADTGAYKVRMILFPGLYCADTAFSTVLVYPKVDAAFSHNDSCSGLPTTFINNSVSTS
                     GPITSTYWTISLNDKTIDSATTNNLTYTFRQAPQSYSVILRITNSEGCATADTQTVNM
                     YHSPYPLAFHDTILTKGTPITLHADDGNYGLNATYNWTPPTGLSDPSSANPVLNSQTE
                     GTWSVTITNEFGCQLRDSVHVKYYTGPDIYLPNAFSPNGDGRNDRLRPVAVGIASAFE
                     FRVFNRGGQLLFLTKRPDEGWDGKIGQHPAWPGTYVWEASGIDFRGKLIVRKGTVELI
                     R"
     gene            1092..2051
                     /locus_tag="VKU83_09200"
     CDS             1092..2051
                     /locus_tag="VKU83_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HLI93774.1"
                     /translation="MRKLTVKNIRTAIPLLLLPAAILAGCVKGTNSSSNSTKSLTATI
                     ESYSSMSILKSILVQTKLDATFNQGGPYTFFSTTDNAFTQYGLTSSYLSSLPDSTLTA
                     MILYGAYSGNLLTTALPQGPNAKIITMGGDSIFVTNGANGIFVNGIQLVQGDILASNG
                     VLDVLAGPLAPPAGNFTQKIAQADTSISFTVAAIARASQGKTNVSNILSNGGPLTFFL
                     PDNDAWRAAGYTSASGITAMNADSVASIVLYHIVAGRLFTSDMLSGATPVGLNQEPFT
                     ISTPTINGNTISGKNNTTKGNLIFTNILVRNGVLHIINILLKP"
     gene            complement(2128..3021)
                     /locus_tag="VKU83_09205"
     CDS             complement(2128..3021)
                     /locus_tag="VKU83_09205"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HLI93775.1"
                     /translation="MKRNRIKNSLLTLFGSVVVLALSSCLKNGQYYDDFSAVNASVDL
                     PLAAANSNAPVAFVYDATVTTTSIPVYVNLASPNTLGKSVTATLALDTAYLNKYNTAN
                     STNFQLIPDSVYTIAGGWNRTIPAGKRLDSMLVTFNFTKMNLAIPYVLPITIASSSEP
                     IEQWNHLMLNPSVKNQYDGIYSIKGYTLRSGDAVKTGNFTGLTMPMVTAGPASLVFGN
                     LQPWADGTGVGIGNPLLAMNTSGGSPYPVTITSPGGASNAPGYNSVYDAPTKTFYISF
                     TWGAGPTSRLAIDTLTYVKAR"
BASE COUNT          767 a          949 c          713 g          673 t
ORIGIN      
        1 cgattgccac gatttcacca ttagcttccc gaacaacagc acccccgatt accacggcta
       61 caactggctc cgcaccttta gctggacctt gggcgatggc gatacttcca ccgccgtcta
      121 cccggtacac acctatgccg ataccggcgc ttataaagtt cgcatgatcc ttttccccgg
      181 cctctattgc gccgataccg ccttcagcac tgtgctggtc tatccaaaag tagatgccgc
      241 cttcagccac aacgacagct gcagcggtct acccaccacg ttcatcaata actccgtttc
      301 cacctccggc cccatcacca gcacctactg gaccatcagc ctgaacgaca aaaccatcga
      361 tagcgccaca accaataacc tcacttacac gttccggcaa gcaccccaat cctacagcgt
      421 tatcctgcgg atcaccaata gcgaaggctg cgctacagcc gatacccaga ccgtgaacat
      481 gtatcattcc ccttatcccc tcgcctttca cgataccatc ctcaccaaag gcacccccat
      541 caccttacat gccgacgacg gcaactatgg cctgaatgcc acctataact ggactccgcc
      601 caccggcctc agcgaccctt ccagcgccaa tcccgttctg aacagccaga ccgaagggac
      661 ctggtcggtg accattacca atgaattcgg ctgccagctg agggattcgg tccacgtaaa
      721 atattatacc ggtccggaca tctatctgcc caatgccttc tcccccaatg gcgacggcag
      781 gaacgaccgg ctcagacccg tggccgtggg gatcgcctcg gccttcgagt tccgcgtctt
      841 caaccgcgga ggacagctcc ttttcctaac caaacggccc gacgaaggct gggacggaaa
      901 gatcggccaa catccggcct ggccggggac ctacgtctgg gaagcctccg ggatcgactt
      961 ccgcggaaag ctcatcgtcc gcaaaggaac cgtagagctc atccggtaaa caggactggc
     1021 cgccagacaa cccggaatcc tttttttcgt attattaatt ggatatattg ctcatcaatt
     1081 acaaaacaca tatgagaaaa cttacagtaa aaaacatccg taccgccatc ccccttttac
     1141 tactgcccgc ggccatcctt gccggctgcg taaagggcac gaatagctcc tccaattcta
     1201 ccaagtcgct gacagcgacc atcgaatctt attccagtat gagcatcctg aaaagcatcc
     1261 tcgtccaaac caaactggat gccaccttta accagggcgg tccatatact tttttcagca
     1321 cgaccgataa tgcgttcacc caatacggtt tgacatcttc ctacttgagc agccttcccg
     1381 atagcacact aaccgccatg atcctgtacg gcgcctattc cgggaatctg ctgaccacgg
     1441 cgctcccgca gggaccgaat gcaaagatca tcaccatggg cggtgactcc attttcgtaa
     1501 ccaacggcgc caatggcatt ttcgtcaacg ggatccagct cgtccagggc gacatcctgg
     1561 ccagcaacgg cgtattggac gtattggcgg gtcccttagc gccgcccgcc ggcaatttta
     1621 cgcaaaagat cgcccaggcc gacaccagca tctcctttac tgtcgctgcg atcgcgcggg
     1681 caagccaggg taaaacgaat gtgagcaaca tactttccaa cggtggcccc cttacgttct
     1741 ttctccccga taacgatgcg tggcgcgccg ccggctatac gtccgccagc ggcataaccg
     1801 ccatgaacgc agatagcgta gccagcatcg tgctttacca catcgttgcc ggccggttgt
     1861 tcacctccga catgctgagt ggcgcaacgc cggtagggtt aaatcaggaa cccttcacga
     1921 tttcgacccc gaccatcaac ggcaacacga tcagcggcaa gaacaacacc accaaaggga
     1981 atctgatctt cacgaatatc ttggtccgca acggagtgct gcacatcatt aatatccttc
     2041 tcaaaccata accggaaaga ccatttccaa aaaataaaaa ggccgcctcc tgcgaagcgg
     2101 ccttctcttt ccctacccga aaatagttta cctcgccttt acatacgtga gcgtatcgat
     2161 agccaaccgt gaagtcggcc ccgctcccca ggtgaaggag atatagaacg tcttggtagg
     2221 cgcatcataa acggaattat aaccaggggc attgcttgct ccaccgggac tcgtgatcgt
     2281 aacaggataa ggagacccgc cggaagtatt catggccagc aggggatttc cgatgcccac
     2341 accggtacca tcggcccagg gttgcaggtt gccaaagacc agggacgcag gtccggcggt
     2401 aaccattggc atcgtgagac ccgtgaaatt acccgtcttg acggcgtcac cagaccgcag
     2461 cgtatagccc ttgatggaat agatgccgtc atactggttc tttacggaag gattgagcat
     2521 taagtgattc cactgctcga tcggctcgga agagctggcg atcgtgatcg gaagcacata
     2581 aggtatggcc aggttcatct tcgtgaaatt aaaggtgacc aacatgctat ccaagcgctt
     2641 gccggccggg atcgtgcgat tccagccacc ggcaatcgta tacacgctgt cggggatcaa
     2701 ctggaaattc gtcgaattag cggtattgta cttgttcaga taagccgtgt ccaacgccag
     2761 ggtcgcggtg accgacttac ccagcgtatt ggggctggca agattcacat ataccgggat
     2821 gcttgtagta gtaacggtgg cgtcgtatac aaaggcgacc ggcgcattgc tgttggccgc
     2881 cgccagcgga agatccaccg aagcatttac agctgaaaaa tcgtcataat attgaccatt
     2941 ttttaggcag gaacttagcg caaggactac caccgatcca aaaagcgtga gcaaactatt
     3001 ttttattcta tttcttttca tgtcttttgt tttaatgatt ttctgtaatt cctaattagt
     3061 ttaaggcatc cagaaaattt tggaattgta cggatccacc tt
//
LOCUS       DATGKE010000540         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1051890, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000540 DATGKE010000000
VERSION     DATGKE010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051890"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1791)
                     /locus_tag="VKU83_09210"
     CDS             complement(325..1791)
                     /locus_tag="VKU83_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HLI93776.1"
                     /translation="MVRKKQWMGILSMVILLTGPAAMGQISVPRLVSDGMVLQREQPI
                     HIWGFGSPGEHVTVKFDGETAEAVTDDNGRWAMALAPRKAGGPYTMDIDGINHIWLKN
                     IMVGEVWFCGGEYPMYRRLGNVKEKYADLISRARQVPIYQFRIETHYDFRGPRRNLSS
                     GRWEAADSASVLSFSELAYLFAIQVYNHYHVPVGLIDASVAEAPAEAWLSPEGPEGLN
                     LFPEYSTLASRYADSSYAPGNGPVNRKAPGGLFNGMVAPADLYTVRGILWCQGDANLD
                     RPQEYQRIFSTLISDWRVHWHDGDLPWIYAQLGPHGAPVDTPQESGRAELRDAQRRVL
                     GLPFTGMAVAADLGERDDGDVADKEELAKRLFLSAEHIAYQKQNMIFSGPLFHSIHIR
                     HNKAILGFDEVNGGLIVKGGGELHGFALAGPDNHFYAAVAAIEGKRVIVTSDHVPNPV
                     TVRYGWADNPQHANLYNRDQLFQDGLPAPAFQAQKLPK"
     gene            1973..3013
                     /locus_tag="VKU83_09215"
     CDS             1973..3013
                     /locus_tag="VKU83_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLI93777.1"
                     /translation="MSDQKEVTIYDLARKLDLSIATISRALKDDPVVSKKTKKRIADL
                     AREMGYRSNHSARNLRTMRTMTIGVIIPRINSYFMSAVIAGIESIANREGYNLIISQS
                     SESAEKERANAATLFNSRVDGLLVSLAYDTGSLSHFEPYFQKRVPVIFFDRVAEHKDC
                     TGILIDNAKAAYDATRHLIRQGRRRIVHITAPSKQNVYTDRLAGYKQALADNQIPYRE
                     DYVVVGNLSQEAGAEAAVTIRAMSPLPDAVFVANDTCAVGCMVAIRQMGIRIPGDIAF
                     VGFNNDPVSTVVEPNLTTVNYPGYEMGQLAARSLIDHLNGAANIRSTNTILLRSELII
                     RDSSVDKKSASR"
BASE COUNT          722 a          890 c          737 g          673 t
ORIGIN      
        1 gatgaacagg caacaggcca tcataacgga taaataacgt ctgctcataa tcgtatggtt
       61 ttggcgacac taatgtagga aaaaagaata agaaaatgca accgattgca gcatggtcac
      121 taagtgaggc ttcatgcgaa gaccgaatac acgcgaaccc gccggaagcc tcactctctc
      181 cctaagtgag gcttcatgca aagaccgaat acacgcgaac ccgccggaag cctcactctc
      241 tcccccagtg aggcttcaca cggaggtcga agttcccgcc aacccgccgg aagcctcact
      301 ctctccaagt gaggcttcat ctatttattt tggcagtttc tgcgcctgga acgccggcgc
      361 cggtaacccg tcctggaaca gttggtcgcg gttatagaga ttcgcatgct gcgggttatc
      421 agcccaaccg tagcgtaccg tcacgggatt gggtacatga tcgctcgtaa cgatcacccg
      481 ctttccctcg atcgcagcga cggcagcata aaaatgattg tccggccccg cgagtgcaaa
      541 gccatgcaat tctccgcctc ccttcacgat caaccctccg ttgacctcgt cgaaaccaag
      601 gattgcctta ttgtggcgga tgtggatgga atggaaaagc ggcccggaaa aaatcatgtt
      661 ctgtttttga tatgcaatat gttccgcaga aagaaataac ctttttgcca gttcttcctt
      721 gtcagcgaca tcgccatcat ctctttcacc aaggtcggca gctacggcca tgccggtaaa
      781 cggcagaccc agaacgcgcc gctgggcatc cctcagttcc gcccgcccgc tttcctgggg
      841 cgtgtcgacc ggcgcgccgt gaggcccgag ttgtgcatag atccagggga ggtctccgtc
      901 atgccaatgt accctccagt cgctaatcag cgtagaaaag atcctttgat attcctgcgg
      961 cctgtcgagg ttggcatcgc cctggcacca caagatgccc cgcacggtgt acaggtccgc
     1021 cggcgcaacc atgccgttga acaaacctcc gggcgccttt cggttcaccg ggccgttccc
     1081 cggcgcataa ctgctgtccg cataccggct tgccagtgtg gaatattccg ggaacagatt
     1141 gagcccctcc ggtccctccg gactcagcca ggcttccgcc ggcgcttccg ctaccgatgc
     1201 atcgatcagg ccgaccggta catgataatg gttatagacc tggatcgcaa acaaataggc
     1261 cagttccgag aaagaaagga ccgatgcaga atcggccgcc tcccatcttc ccgaagacag
     1321 gttccgccgc ggtcccctga agtcataatg tgtttcaatc ctgaattgat agataggcac
     1381 ctggcgagcc ctggagataa gatcggcata cttttccttc acattcccca gccgtctgta
     1441 catcgggtat tctccgccac aaaaccagac ttcaccgacc atgatgttct tcagccaaat
     1501 atggttgatg ccatctatat ccatcgtgta gggtcctccg gcctttctgg gagccaatgc
     1561 cattgcccat cttccattgt cgtcggtaac agcttccgca gtctcgccgt caaatttgac
     1621 cgtgacatgc tcgcccggcg agccgaaacc ccagatatgg atcggctgtt cccgctgcaa
     1681 caccatgccg tcactaacga gtcttggaac gctgatctga cccattgccg ccggccctgt
     1741 caacagtatc accatcgaca agatccccat ccattgcttt ttacgaacca tgaataccga
     1801 tttggttcgc aatatcgggt tttcgatcga atagtccgcg gccggcggat caggtcgata
     1861 ttggcgcccg gcgtttgaag gactgcaaat gattgcgaaa atataaccga ttacattttt
     1921 tttcgaaaaa taaattaaat ttatcgctgc ctaagggatg taaacgattg ctatgagcga
     1981 ccagaaagaa gttaccattt acgacctggc ccgaaagctg gacctctcca tcgctacgat
     2041 cagcagagca ttgaaggacg atcctgtcgt cagcaaaaaa acaaaaaaac ggattgcaga
     2101 ccttgcccgg gaaatgggct accgcagcaa ccattccgcg cgcaatctcc gtacgatgcg
     2161 caccatgacc atcggggtga tcatcccgcg gataaacagt tattttatgt cggcagtcat
     2221 cgcaggcata gagagtattg ccaaccgcga aggatataat ttaatcatta gccagtcgtc
     2281 tgaatcagcg gagaaagaac gcgcgaatgc tgccaccctc ttcaatagcc gggtcgatgg
     2341 actactggtt tcgctggcct acgacaccgg atccctgtca catttcgagc cttatttcca
     2401 aaaaagggtc ccggttattt ttttcgaccg tgtcgcggaa cataaagact gcaccggtat
     2461 cctgattgat aacgctaaag cggcttacga cgccacccgt cacctcatcc gtcaggggcg
     2521 gcgacgcatc gtccatatca cggcgccctc taaacagaac gtatatacag atcggctcgc
     2581 cggttataag caggcgctcg ctgataacca gatcccttat cgcgaagact atgtggtcgt
     2641 gggtaatctc agccaggaag ccggcgccga ggcagccgtg acgatccgcg ccatgtctcc
     2701 gcttccggat gcagtctttg tggccaatga cacctgcgcc gtgggctgca tggttgccat
     2761 ccgtcaaatg ggtatccgga tcccgggaga tattgcattc gttggattca acaacgaccc
     2821 cgtgtccaca gtggtcgagc cgaaccttac caccgtaaac tatcccggct atgaaatggg
     2881 ccagttggcc gcccgtagtt tgattgacca cctcaacggt gcggccaaca tccgttccac
     2941 caatactatc ctgcttcggt ccgaacttat catccgagac tcttctgtcg acaaaaaatc
     3001 cgcttcacga tgaagaaagt cg
//
LOCUS       DATGKE010000541         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1106593, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000541 DATGKE010000000
VERSION     DATGKE010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106593"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..550)
                     /locus_tag="VKU83_09220"
     CDS             complement(62..550)
                     /locus_tag="VKU83_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93778.1"
                     /translation="MEQIQQKDKRTLVLQIIQPGLEGLMDGSVSSLAPLFATALATKN
                     NHTTFLVGMAVAVGAGISMAFSEALSDDGKLSGRGNPISRGAICGLMTFVGAAGHALP
                     FLLSSFHAAMTVAIVVVIVELFAIAWVRNRFMDTALWKAVGQIVIGGIVVFIAGILIG
                     RS"
     gene            671..1039
                     /locus_tag="VKU83_09225"
     CDS             671..1039
                     /locus_tag="VKU83_09225"
                     /inference="COORDINATES: protein motif:HMM:NF026420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HLI93779.1"
                     /translation="MFLLILNIVFCLAFLYFAYLNLNDVDPWLWVPIYVAAAACCGLT
                     VFGMYFPRVYLVLIALYLLYAVILFFAKDGVRDWIVKYKSPSIVESMQAEKPWIEKTR
                     EFFGLLIISGALVVDYFVVR"
     gene            complement(1052..1240)
                     /locus_tag="VKU83_09230"
     CDS             complement(1052..1240)
                     /locus_tag="VKU83_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93780.1"
                     /translation="MKYVILSTDWHRPVYFCAHKESGFTVSTDPKKAQVFTSMKAAQV
                     ACTAFSEASGMKCEIKKQ"
     gene            1647..1835
                     /locus_tag="VKU83_09235"
     CDS             1647..1835
                     /locus_tag="VKU83_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93781.1"
                     /translation="MMRNPWELNQCTSLDKGDYGFMISEIQKLNENFGFKILGGCCGT
                     DAVFIDQVARSIAKIHND"
     gene            complement(1896..>2087)
                     /locus_tag="VKU83_09240"
     CDS             complement(1896..>2087)
                     /locus_tag="VKU83_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93782.1"
                     /translation="QIGTNIDIITDSIMLFSGSLVVQLFLATLGIGLRRFFYRPKLLT
                     SLNVLSGIGIIVLSVVKYL"
BASE COUNT          527 a          507 c          519 g          534 t
ORIGIN      
        1 ttgtttttgg tcaattttgc ggaaccgccc attaccggat cagcagcctg gccggccccg
       61 gtcaacttcg accaatgagg ataccggcaa taaagaccac gatgccgccg atgacgatct
      121 gaccgacggc tttccaaagc gcggtgtcca taaagcggtt gcggacccat gcaatcgcga
      181 acaattccac gatcactaca acgatggcaa ccgtcatggc ggcatggaag gaactgagaa
      241 gaaagggcag cgcatggcct gcagcgccca caaaggtcat caatccgcaa atggcgccgc
      301 ggctgatggg attcccccgg ccgctcagtt tgccgtcatc cgacaaggct tctgagaacg
      361 ccatgctgat tcccgcgcca acggcgacgg ccattcccac caggaacgtc gtatgattgt
      421 tctttgtcgc cagcgccgtc gcgaataatg gcgccagcga ggaaaccgag ccgtccatca
      481 gcccttccaa tcccggctga ataatttgaa ggaccagggt ccgtttgtct ttttgctgga
      541 tttgttccat atcatttatt ggtgcgggtg tggggcaggc aatacccaaa tgggatatcc
      601 tttgtccgga gattcataaa ccatgcccgg ctgaatcgat cagcgccgaa ttgtcttata
      661 ttagtggtct atgttccttc ttatcctcaa cattgtcttt tgcctggcct tcctctattt
      721 cgcctacctg aacctaaacg acgtcgaccc ctggctgtgg gtgcccatat atgtcgccgc
      781 cgcagcttgc tgcggactaa cggtattcgg catgtatttt ccccgggtat acctcgtgct
      841 gatcgcactg tatctcctat atgccgtcat cctctttttc gccaaggacg gcgttcgcga
      901 ctggatcgtc aaatacaaat caccaagtat tgtcgaaagt atgcaggccg aaaagccctg
      961 gattgagaag acccgcgagt tcttcggcct gctgatcatc agcggagcgc tggtcgtgga
     1021 ttattttgtg gtgcggtgag cgcaggtccc gctactgttt cttaatctcg catttcatcc
     1081 cggatgcctc gctaaaggcg gtgcacgcga cctgtgccgc cttcatactc gtgaagacct
     1141 gggctttttt agggtcggta ctgacggtaa agccgctttc tttgtgggcg cagaagtata
     1201 ccggccgatg ccaatcggtc gataagatga cgtatttcat tctcctattg gtgtcggccg
     1261 aagcaggata tgtaaccggt ctgccggcct gagctagacg aagatttgag cttgaagggc
     1321 tttttgcaga gcgaattacg caaagttgta aagtccgaat ggatcctgct ataaatcatg
     1381 cgggattatt tatgatgatc aggggaaggc cattttagct gatatttata aacaatatat
     1441 cggattgtcg attaaatatc agatcccgat gatgctgatg acgccgaccc gtcgggtgaa
     1501 tgccgacagt gttaaaaggt cggtattcca gggaaagaac atcatcgaag attcagttgc
     1561 atttctaatt gaactgcgag ataggtatcc atcttgccgc gaaaacatct tgatcggagg
     1621 gttattgggt tgtaaaggag atgcctatga tgcggaatcc ctgggaattg aatcaatgta
     1681 caagcctgga caagggagat tatggcttta tgatcagcga aatacaaaaa cttaatgaaa
     1741 atttcggctt taaaatactg ggcggttgct gcggcacaga tgcggttttt attgaccagg
     1801 ttgcccgttc catcgccaaa attcataatg attaagccag tcaggtctgg gtacgaaaag
     1861 ccaggcagga gcttataatc ctaagccatg aaaaatcaga gatattttac tacacttaat
     1921 acaattattc caatcccact taacacatta agggaagtaa gcagtttggg ccggtaaaaa
     1981 aagcgacgta aaccgattcc taatgttgct aagaacaatt gtacaaccag gcttccgcta
     2041 aacaacatga tactatctgt aataatatca atattagtac caatctg
//
LOCUS       DATGKE010000542         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_368305, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000542 DATGKE010000000
VERSION     DATGKE010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368305"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VKU83_09245"
     CDS             <1..356
                     /locus_tag="VKU83_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93783.1"
                     /translation="AYDASASAVWAIKQFTYLFPEYHDKKVLVVNVRNSRDEFIEESF
                     KMKEWLREHYQDVEFHVLTGNPSDQLFAFLLERKNSFVVMGAYGRGILSRFLRPSSAR
                     LIVRTLNLPIFVTHL"
     gene            384..2768
                     /gene="ppsA"
                     /locus_tag="VKU83_09250"
     CDS             384..2768
                     /gene="ppsA"
                     /locus_tag="VKU83_09250"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HLI93784.1"
                     /translation="MANFIRKFRDIRLTDVAVVGGKNASLGEMFSQLAATGVRVPDGF
                     ATTAEAFRYFLQWNKCGPALNSLVAGLDRKDFSNLAETGKAARSLLLQAVLPPELESE
                     ILAAYEDLGSGRPIAVAVRSSATAEDLPAASFAGQHESYLNIQGSQALLNAVRSCYAS
                     LYTDRAIKYREDNGFAHAAVALSIGIQKMVRSDKACSGVGFTLEPESGFRNIIHLAGV
                     WGLGENIVQGASTPDEFLVFKPSLRAGKRAIIQKTCGEKERMLVYTEEGGGTVNLYTS
                     AARREQWVLTEEEITSLARWALTIEEHYGRPMDFEWAKDGETNEIFIVQARPETVHSR
                     KNPFCIKEYTLVQKGECLARGEAVGEQIVSGTARILSSPQQEDQVQAGEIIVTDITSP
                     DWDPVLKKAGAIITNKGGRTSHASIVARELGVPAIVGTHDATEKIRDGEPVTVSCCEG
                     KTGFVYRGKAAWTERQISLDKLRKPQHVAPMLILADPEKAFELSFYPSAGVGLMRMEF
                     IVTHSVQIHPMALVRFDEVRDSQARREIEQLTSRYSDKKKYFVDQLASGIATIAAAFF
                     PRPVILRTSDFKTNEYANLLGGLQFEPQEENPMLGFRGASRYYNPLYKEGFKLECEAI
                     RVVRDEMGLTNLKVMIPFCRTVEEGRQVIAVMREYGLPQHVNGLEIYVMAEIPSNVLL
                     ATEFAAIFDGFSIGSNDLTQLTLGIDRDSSIIAGLFNEQNAAAKKIIGNMISAAKQAG
                     ARIGLCGQAPSDYPEFAQFLVDQGIDSISFNADALLRGIENILKAEEKSIVHIL"
     gene            2785..3144
                     /locus_tag="VKU83_09255"
     CDS             2785..3144
                     /locus_tag="VKU83_09255"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLI93785.1"
                     /translation="MEEFIKFENLIDHTREYVNTRVHEAKLTVAKKASGAAAQIIAGA
                     VVGIIFLISLLFASAAAAYRLGMEWGRLWLGLLAVAAFYLLLGLLVWAARERLIRIPI
                     MNSLIHQLFKNEQDEED"
BASE COUNT          755 a          884 c          924 g          672 t
ORIGIN      
        1 tcgcgtacga tgcttcggct tctgcggtct gggcgatcaa gcaattcact tacctgttcc
       61 cggaatacca cgacaaaaag gtcttggtgg tgaatgtccg caacagcaga gatgagttca
      121 tcgaagaatc cttcaagatg aaggagtggc tccgcgaaca ctaccaggac gtcgaattcc
      181 acgtcctgac cggcaatccc tcggatcagc tctttgcctt cctgctggaa cggaagaaca
      241 gcttcgtggt gatgggtgct tacggccggg gaatattgtc ccggttcctg cggccaagca
      301 gtgcgcggct gatcgtccgt accctgaacc tgccgatttt cgtgacccat ctctgaaaaa
      361 aatgggcttt aaattctatt gtcatggcaa attttatcag gaaatttcgc gatatccggc
      421 tcacagatgt agctgtcgtg ggcggcaaga atgcttccct gggcgagatg ttttcgcagc
      481 ttgcggcaac gggagtccgc gtaccggacg ggtttgctac aacggccgaa gcattccgct
      541 acttcctgca gtggaataaa tgcggccccg ctctcaatag cctggtcgcc ggcctcgacc
      601 gtaaagattt cagcaacctg gccgaaaccg gtaaggccgc aaggtccctc ttgctacagg
      661 ctgtcttacc gcctgaactc gaatcggaga tcctcgccgc ctatgaggac ctgggttccg
      721 gccggccgat cgcggtggcg gttcgcagca gcgcaacagc ggaagatctg ccggctgcaa
      781 gttttgccgg ccagcatgaa tcctatttga acattcaggg ttcgcaggcc ctcctgaatg
      841 cggtcaggag ctgttatgca tccctgtaca ctgaccgtgc catcaaatac cgcgaggaca
      901 atgggtttgc tcatgctgcc gtggccttgt ccatcggcat ccagaaaatg gtccggtccg
      961 acaaggcctg ctccggtgtg ggatttacgt tggagcccga gtctggcttc cgcaatatta
     1021 ttcacctcgc gggggtgtgg ggtcttggcg aaaacatcgt gcagggagca tcgaccccgg
     1081 acgaattttt ggttttcaaa ccttcgttgc gtgccggaaa acgcgccatc atccaaaaga
     1141 cctgcggtga aaaggagcgg atgctcgttt atacggaaga gggaggcggt acagtaaatt
     1201 tatatacttc cgccgcccga cgcgagcaat gggtcctcac cgaagaggag atcacttcgc
     1261 ttgcccggtg ggcgcttacc attgaagaac attatggccg cccgatggac ttcgagtggg
     1321 ccaaggacgg cgagacgaac gagatcttta ttgtgcaggc gcgacctgaa accgtccact
     1381 cgcgcaagaa cccattttgc ataaaagaat ataccctggt ccaaaaaggg gaatgccttg
     1441 cgagagggga agcggtaggc gagcagatcg tcagcggcac ggcgcgcatt ttgtcttccc
     1501 cccaacagga ggaccaggtg caggcgggcg agatcattgt gaccgatatc accagccccg
     1561 attgggatcc ggtgctgaaa aaggcaggcg ccatcatcac caataagggc ggccgcacca
     1621 gtcacgcgtc gatcgttgca agggaattgg gcgtgccggc cattgtaggg acgcacgacg
     1681 ccacggaaaa gatcagggac ggcgaaccgg tcaccgtgag ctgctgcgaa ggaaagaccg
     1741 gctttgtgta tcgcggaaaa gctgcctgga cggaacggca aatcagcctc gacaagctca
     1801 ggaagccgca acacgtcgct ccgatgctca tcctggctga cccggagaag gcatttgagt
     1861 tgtccttcta tcccagcgcc ggcgttggac tcatgaggat ggagtttatc gtcacgcact
     1921 cggtgcagat ccatccgatg gcgctggtca gattcgacga agtgcgcgac agccaggcca
     1981 ggcgggaaat cgaacaactg acctcccggt attccgacaa gaagaaatat tttgtcgatc
     2041 agctggcttc aggtatcgcc acgatcgctg cggcattttt tccacgcccg gtgatcctcc
     2101 ggacaagcga tttcaagacg aatgaatacg ccaatctgct cggcggcctg caattcgaac
     2161 cacaggaaga aaacccgatg ctgggcttcc gcggagcctc gaggtattat aaccccctat
     2221 acaaggaagg attcaagctg gaatgtgagg cgatccgggt ggtccgcgac gaaatgggcc
     2281 tcaccaatct caaggtgatg atcccgttct gccggaccgt agaagagggt cggcaggtca
     2341 tcgccgtcat gcgggagtac ggtctgcctc agcacgtaaa cgggctcgaa atatatgtca
     2401 tggcggaaat cccgagcaat gtcctcctcg ctaccgagtt tgccgcgatc ttcgacggat
     2461 tttccatcgg cagcaacgac ctgacccaac tcaccctcgg cattgaccgg gactcgtcca
     2521 tcatcgccgg gctgttcaac gaacaaaatg cagccgcaaa gaagatcatc ggaaatatga
     2581 tctcggccgc caagcaggct ggggccagga tcgggctgtg cggccaggcg cccagcgatt
     2641 atcctgagtt cgcgcaattc ctcgtggacc aggggatcga cagcatttca ttcaatgccg
     2701 atgccctgtt gagaggtatc gaaaacatcc tgaaagcaga agagaaaagc atcgtgcata
     2761 ttttataaca tcaaatcagc atcaatggaa gagtttatca aattcgaaaa cctcatcgat
     2821 catacccggg agtacgttaa tacccgggta cacgaggcca aactcacagt ggccaaaaag
     2881 gctagcggag ccgcggccca gatcattgcg ggtgcggtcg ttgggattat attcctgatc
     2941 agccttcttt ttgccagcgc cgcggccgct taccggttgg gaatggaatg gggccggtta
     3001 tggctgggac tgcttgcagt cgccgctttt tatctgttgc tcggcttact ggtctgggcg
     3061 gcaagggagc gcctgatccg cattcccatc atgaattcgc tgatccatca actctttaaa
     3121 aacgaacaag atgaggaaga ttaaacgcct tagccacctc cggcgggaag aacgccggct
     3181 gcgcgaacac cgggccgaac tggagcgaaa tattcaaagc gattggctgc tgatc
//
LOCUS       DATGKE010000543         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_943310, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000543 DATGKE010000000
VERSION     DATGKE010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943310"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VKU83_09260"
     CDS             complement(<1..809)
                     /locus_tag="VKU83_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLI93786.1"
                     /translation="MDPLVAEYPASGLTTEEAEVLAVKFGSNEISADRKNPVFRMAWD
                     IIREPMFAMLLLACVVYLILGELQEGGLMIIAMVFVGAISFYQEIKSSNALSALRQYT
                     EPRVLVIRNGEEQTIPSQYLVPGDVIAVEEGEMIPADGVLLQSNDLSVNESILTGESF
                     PVEKTAQSGDKRIFQGTLVNSGRGYAKVIATGNSTVLGKLGRSISSAPVAKTLLQKQI
                     SRFVRIMALVGGIVFCLLLALTYWHTGEFLTSLLAALTLVMAIVPEEIPVA"
     gene            complement(968..1810)
                     /locus_tag="VKU83_09265"
     CDS             complement(968..1810)
                     /locus_tag="VKU83_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93787.1"
                     /translation="MKKEFKILVPTDFSAAARAGMRFAVQWARQQKARLIFAHVLDMI
                     QYEEWSDRKYAFYKAAQTKLMSRRLRSVVGEVMRHRTTARSNFDARLVEGVGVNQVLA
                     DLSHEKDLDLICMGTNGAGTVQKMLGTNTRKLIFRSAVPVVAVPAGYRSKPIKKILYA
                     TDLVDYSGELAKILNLTRPLGAKITVVHFATIGELKLDPSLISKVWKEQYGCKIDLVY
                     GNMDPTLTLADNLQLAVRRLKPSMVVMFTDRQRTWFQRLLYPSQSGRMSSLSKVPLLV
                     MAKQ"
     gene            2049..2234
                     /locus_tag="VKU83_09270"
     CDS             2049..2234
                     /locus_tag="VKU83_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93788.1"
                     /translation="MIIHPDLVLSWGAIRRKFQKDRIAFCEEGQDLEAGRALEEGRDL
                     FYYHAGAVPPEKGTVYV"
     gene            2227..>3259
                     /locus_tag="VKU83_09275"
     CDS             2227..>3259
                     /locus_tag="VKU83_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93789.1"
                     /translation="MSEELAGRLEAPDKRMLQGWAKVSVANLLVLSLLGLALRYKATF
                     SLPAINYKFLLNAHSHFAFTGWVTSVIYTVLLYMLAQSGVRLSSAYRNLFLINQISAY
                     GMLICFTWQGYGPASIVASALSVLVSYGFSILYWLDLARTAWPRAVRHGVRFALAFLV
                     LSSAGPFLLVYSMSHDAGMAFYYNSIYLFLHFQYNGWFSFSAIAAFFWMSRMYGWEIS
                     RRASTSFVVLMGVACVPAYCLSLLWTEPPVWLWAAAGAAALLQLSALRILLLAMWRRK
                     RAGSARDLPDSVRILWTIALVALAIKLILQGLSAIPAIGRHAFGYRPVIIAYLHLVLL
                     GFISVFFLGF"
BASE COUNT          673 a          947 c          846 g          793 t
ORIGIN      
        1 aatgccacgg ggatctcctc cggcacaatg gccataacga gggtaagggc cgccagcaaa
       61 ctggtcaaaa attccccggt gtgccaatag gttaatgcca gcagaagaca aaaaacaatt
      121 ccgccaacga gcgccatgat ccggacaaaa cggctgatct gtttttggag cagggtcttg
      181 gcgaccggcg ccgacgatat tgacctgccc agtttcccga ggacggtact gttgcctgta
      241 gcgatcacct tcgcatagcc ccgcccggaa tttacaaggg tcccctggaa aatccgctta
      301 tctccgcttt gtgccgtttt ttcaaccgga aaggattcac ccgtgaggat ggattcgttt
      361 acgctgagat cgttcgactg cagaagaacc ccatccgccg ggatcatctc accctcctca
      421 accgcaatga catcgccggg aaccaggtac tgtgaaggga tcgtttgctc ctcgccattt
      481 cggatcacca gcacccgcgg ctcagtatac tgccggaggg ccgacaatgc attggagctc
      541 ttgatctcct gataaaaaga gatagccccc acaaacacca tcgcaatgat catcaatccg
      601 ccttcctgca attctccgag aatgaggtaa acaacacagg ccagcaggag catggcaaac
      661 atcggctccc tgataatatc ccaagccatc cggaataccg gattctttct atccgcagaa
      721 atctcgttac ttccaaattt aacggccaga acttcagcct cctcggtagt gagtcccgat
      781 gcgggatatt ccgcaaccaa cggatccata tgggtcattt tgggtttcta ttctcttcgc
      841 tgcatgctaa tccaaggtag tcaatgccgt ggccggcaat attgaccgat gcatccaccg
      901 cacatgatta ttgtcaggaa atccgcatct tccgcgcagg aacgaagaat aactgcgccg
      961 ggcctgccta ctgtttcgcc atgaccaata agggaacctt tgagagcgaa gacatccgcc
     1021 cggactggct gggatataac aaccgttgaa accatgtccg ctgcctgtcg gtaaacatca
     1081 cgaccatcga cggctttaac ctcctgactg ccaactgaag gttatcggcc aacgtcaatg
     1141 tcgggtccat attgccgtat accaggtcaa ttttgcatcc gtactgttcc ttccatacct
     1201 tagatatcag ggaagggtcc aacttcagtt caccaattgt agcaaagtgg acgaccgtga
     1261 tttttgcacc gagcggccgg gtcagattca gtattttcgc cagttcgccg gaatagtcga
     1321 cgagatccgt ggcgtacagg atctttttga tcggtttcga gcggtatccg gccggaacgg
     1381 cgaccaccgg aaccgccgac ctgaaaataa gtttccgggt gtttgttccc agcattttct
     1441 gcaccgtgcc ggcgccgttg gttcccatgc aaatgaggtc gaggtccttt tcgtggctga
     1501 ggtcggcaag cacctgattg acgccaaccc cttccaccag ccgcgcatcg aaattggagc
     1561 gcgcagtcgt cctgtgacgc ataacctccc ctacgaccga ccgaagcctg cggctcatca
     1621 acttcgtttg agcggccttg taaaaggcgt atttacggtc gctccactct tcgtactgga
     1681 tcatgtcgag gacatgtgcg aatatcagcc gggccttttg ctggcgtgcc cattggacgg
     1741 caaatcgcat tcccgcccgg gcagcggccg aaaaatccgt cgggaccagg atcttgaatt
     1801 cctttttcat ttcggttagt acttgaaacg cgaagttcgc gcgccgaaac ggtaactctc
     1861 atgacctggg ttaaacgtcc ggttgactat gatcgccgga acgccgggat gaccagggca
     1921 ttctcccatt acaatattac cggacctgac gaaagtcatc actggctccc gcgctcctga
     1981 tcttaatcag ccggacatcc gaccgcggtc atccggccgg ggccatgggg cgcatatttt
     2041 tggccatcat gatcatccac ccggacctcg tgctatcctg gggcgccatt cgccggaaat
     2101 tccaaaagga caggatcgct ttttgcgaag aaggccagga cttagaagca ggccgggcct
     2161 tagaagaagg ccgggactta ttttattacc acgcgggcgc cgtaccaccc gaaaaaggaa
     2221 cggtctatgt ctgaggagct ggcgggcagg ttagaggccc cggacaagcg tatgctgcaa
     2281 ggctgggcaa aagttagcgt cgcgaacctg ttggtcctgt cgcttctcgg cctggcactt
     2341 cggtacaaag ccaccttttc ccttccggcc atcaattata aattcctgct caatgcacat
     2401 tcgcacttcg cttttacggg ctgggtgacc tccgtgatat acaccgtatt gctgtatatg
     2461 ctggcgcaat ccggggtccg cttatcctct gcgtaccgta atctattcct catcaaccag
     2521 atttccgcct atgggatgtt gatttgcttc acgtggcagg gttatggccc ggcatcgatc
     2581 gtcgcttccg ctctatcagt tctcgtttcc tacggattca gcatccttta ttggctggat
     2641 ctggcccgca cggcctggcc ccgggcggtc cggcacggcg tccgcttcgc attggccttc
     2701 cttgtcttgt caagcgcagg gcccttcctg ctggtgtaca gcatgtcgca tgacgccggt
     2761 atggccttct attacaactc catctacctg tttctgcatt ttcagtataa tggctggttt
     2821 agcttctcgg caattgccgc ttttttttgg atgagccgga tgtatggctg ggaaatttcc
     2881 cggcgggcat ccacgagctt tgttgtcctg atgggagtgg cctgtgtgcc tgcctactgt
     2941 ctgtccttgt tgtggacgga gccgccggtc tggttgtggg cggctgccgg ggccgccgcg
     3001 ctgttgcaac tgagcgcgtt acgcatcctg ctcctggcta tgtggcgccg caagcgcgcc
     3061 ggatccgccc gtgacctgcc ggattccgtg cggatacttt ggacgattgc gctggtggcg
     3121 ctggccatca aacttatttt acagggatta tcggcaatcc cggccattgg ccggcatgcc
     3181 ttcggctacc gtcccgtcat catcgcctat ctgcacctgg tcctcctggg attcatcagt
     3241 gtattttttc tgggtttcc
//
LOCUS       DATGKE010000544         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_534269, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000544 DATGKE010000000
VERSION     DATGKE010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534269"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..923)
                     /locus_tag="VKU83_09280"
     CDS             complement(186..923)
                     /locus_tag="VKU83_09280"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HLI93790.1"
                     /translation="MKSDVNGMDSLPGLTTTEASLELRYAPHEQFYIGKLYRTPITNK
                     YPILDFSYTRGIKGFWNGQYDYNSFDFNIFKRFYLSQLGYTDVTADFGYLLGKVPFAL
                     AYIHHANQTYAYQLQSYNLMNFLEFVSDHYATAYVDHYFNGFFFNKVPLLKKLKWREV
                     VEAKVLFGGLRDENNPAKTPDQVQFPLTNGKMETFALGSTPYIEAGFGIANIFKLVRV
                     DFIKRFTYLDHPDIAKYGIRFRTHFDF"
     gene            complement(990..2723)
                     /locus_tag="VKU83_09285"
     CDS             complement(990..2723)
                     /locus_tag="VKU83_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HLI93791.1"
                     /translation="MYVKIWIVLLCGVCLNQSGQAQEGVLRGTVLDSISRTPISSASL
                     AIKGLAGGTRTNPEGKFRITIVRRKLDLTVTSVGYNPVTIHLDSVPDREIIIALTRRY
                     HTLGGVTVRNKKLRYRNKDNPAVDLIRHVIDNKAKNRMEAYSYASYEKYEKLQLSIDK
                     SNASKLGQNKLMKPYHFLVENGDTTKLEGRTLSPVYLEETLSDVYYRKSPEKTKTIVL
                     GKRKVDFGEFIDMQGVSSFLNRLYQDFDVYDNNISVFTNLFLSPIADLAPTFYMYFIE
                     DTTVVEGHQVVRLSFRPRNPNDLLFRGTMWVTLDSNYAIQKLRLLVSKSINFNFVREM
                     KIDQDFERLSNGRYLLAKSDVLGDFGINKNGTGIFGERSVSFKNFEIDTPIPDSIFKG
                     PPLVMTDSAANHPDSFWVKARHDTLTYAESKVYANIDSLVHMKSYRRLLDWGTLLLAG
                     YKQAGPFEIGPASTFYSFNPVEGFRLRFGGRTTMRLSKRYYSEAYTAYGFKDQKWKYF
                     LSGTYSINDKSVYSYPQNFIRVSYQKDTKIPGQDLQFVQEDNFLLSFKRGPNDKWLYN
                     KIFRVAYLDEL"
     gene            complement(2788..3180)
                     /locus_tag="VKU83_09290"
     CDS             complement(2788..3180)
                     /locus_tag="VKU83_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HLI93792.1"
                     /translation="MITFTKAQITSLVATGVDFLVYYLLLRWAGLPSPAATGRVTFYG
                     ATGTLCGGVAHFMISRNWVFRAQEGKWAAQVNRYLMVWMGNLALNAAGIYVLSRAGLS
                     PILAKVITATSVAVLYNYTLQRYFVFNR"
     gene            complement(3177..3650)
                     /locus_tag="VKU83_09295"
     CDS             complement(3177..3650)
                     /locus_tag="VKU83_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HLI93793.1"
                     /translation="MTVRIHRLISTCLGIGYIPKGGGTVAALVCCIAWYLAFGGGNDH
                     IWPPLLITLSLLALGTYSSGKVEPGWGKDSYRVVIDEWVGMSFTLLFVPVQWPYLLTG
                     LLLFRAFDIVKPFYVRRMEELKGGWGVMMDDVLAGIYANLVLQLVVVAVHFVKTR"
     gene            complement(3647..>4489)
                     /locus_tag="VKU83_09300"
     CDS             complement(3647..>4489)
                     /locus_tag="VKU83_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HLI93794.1"
                     /translation="LVMVWCGSTEKYIEVADVHKTISAFEKGLKENDPRIAPSMIYAY
                     AALRSGVPFLNGAPNLTVDIPALVELAKRTESPIGGKDFKTGQTLMKTILAPGLQARA
                     LGVKGWFSTNILGNRDGYVLDDPDNFRTKEVSKLSVLEDILRPDLTPELYGELYHKIR
                     INYYPPHGDNKESWDNIDIFGWLGYSMQIKINFLCRDSILAAPLVLDLALFGDLAKRA
                     GLSGVQEWLSFYFKSPQHAPGCRPEHDIFKQLTTLQNALRRLMGEELVHPDGLTYAEE
                     EISA"
BASE COUNT         1037 a         1198 c         1173 g         1081 t
ORIGIN      
        1 ttcagtatca agccgatcgt ggtgaccatg tttggggtta gcccggcttt gatcaacaac
       61 cgtactacgg ggttgatgat cttgtaaatc cctagctgaa gtttagtccg ggtggtcatt
      121 tttcccttgg gttcttgttg tttggcaact gccaaagata ctatcttcat gctgaatttc
      181 aggttttaaa agtcaaaatg tgtgcggaac cggatgccgt atttcgcaat gtccggatga
      241 tcgagatagg tgaaccgttt gatgaaatcg acgcggacca gcttgaagat atttgcaatt
      301 ccaaagccag cctcgatata gggggtgctt cccagtgcaa aggtttccat tttgccattg
      361 gtaaggggaa attgtacctg atccggcgtt tttgcgggat tgttctcgtc cctcaggcca
      421 ccgaacagga cttttgcttc gacaacttcc cgccatttta attttttcag cagcggcact
      481 ttattaaaaa agaagccgtt gaaatagtgg tctacgtacg cggtagcata atgatcgctg
      541 acaaattcga ggaagttcat caggttatag gactgcaact ggtaggcata ggtctggttc
      601 gcatgatgga tataggcgag tgcaaagggg actttcccga gaagatagcc gaagtctgcc
      661 gtcacatcgg tgtagccgag ctgggacaga tagaaccgtt tgaagatgtt aaagtcaaaa
      721 ctgttgtagt cgtactgacc gttccaaaaa cccttaatcc cgcgggtata gctgaaatcc
      781 aggatcggat acttattggt gatcggcgtc cggtacaatt ttccgatata gaactgttcg
      841 tgcggcgcat accgcagctc caaagaggcc tccgtcgtcg tgaggccggg cagggagtcc
      901 atcccgttca catccgattt cacataggcg atcgagccgg ccggcgactg ctcccagtgt
      961 ttgaatccga aattgtacga gaaatgactc tagagctcgt cgaggtaggc gaccctgaag
     1021 atcttgttgt acagccattt atcgtttggc ccgcgcttga acgacaggag gaaattgtcc
     1081 tcctgcacga actgcaggtc ctgccccggg atcttggtgt ccttctgata agagacgcgg
     1141 ataaaattct gcggatagct gtaaaccgat ttgtcgttga tcgaatatgt gccgctcagg
     1201 aaatacttcc atttttgatc cttgaagcca taagccgtat aggcctcgga gtaataccgt
     1261 ttgctgagtc gcatcgtcgt ccggccgccg aagcgtagcc tgaacccttc cacgggatta
     1321 aaactataga atgtgctcgc cggcccgatc tcaaaaggcc ctgcctgctt gtagccggcc
     1381 agcagcagcg ttccccagtc caacaggcgc cggtaggact tcatatgcac gaggctgtcg
     1441 atattggcat acaccttcga ctccgcgtag gtcagggtat catgccgtgc ctttacccaa
     1501 aagctgtccg gatggttggc cgcgctgtcc gtcatgacga gcggcggtcc tttgaaaata
     1561 ctgtctggga tcggcgtatc gatctcgaaa tttttgaagg aaacggagcg ttcgccgaag
     1621 atgccggtcc cgttcttgtt gatcccgaaa tctccgagga cgtcgctctt cgccagcagg
     1681 taacggccgt tggataacct ctcgaaatcc tggtcgatct tcatttcgcg gacaaaattg
     1741 aaattgatgc tcttgctaac cagcaaccgc agtttctgga tcgcatagtt gctgtccagc
     1801 gtgacccaca tggtgccgcg aaacagcagg tcgttcggat tccgtggccg gaaagaaagc
     1861 ctgaccacct ggtggccctc cacaaccgtc gtgtcctcga taaaatacat ataaaacgtc
     1921 ggggccaggt ccgcaatggg actcaggaaa aggttggtga aaacggagat attgttgtca
     1981 tagacatcga aatcctgata aagccggttg agaaaagacg agactccctg catgtctata
     2041 aattccccga aatcgacttt tcgctttccc agcacgatcg tcttggtctt ttccggagat
     2101 ttccggtaat aaacgtccga cagggtctcc tcgagataga ccggggaaag cgtgcgaccc
     2161 tccagtttcg tcgtatcccc gttttccacc aggaaatggt aaggcttcat cagcttgttc
     2221 tgacccagtt tcgaggcgtt cgatttgtcg atggacagct gcaacttctc gtatttttca
     2281 tatgacgcat agctgtacgc ttccatccgg ttcttcgctt tattgtcgat cacatgccgg
     2341 atcagatcga ccgccgggtt gtctttgttc cgatagcgca gtttcttatt cctaaccgta
     2401 accccaccca gcgtgtgata ccggcgggtc aatgcaatga tgatctcccg gtccggcacg
     2461 gaatccaggt ggatggtgac cggattatag cccacgctgg tcacggtcag gtccagcttc
     2521 cggcggacaa tggtgatcct gaacttgcct tccggattgg tcctggtgcc gccggccagg
     2581 cccttaatgg caaggcttgc gctggaaatc ggcgtccggc tgatcgaatc caggaccgtg
     2641 ccgcgcaaga ccccttcctg cgcctggccc gattggttta ggcaaacgcc gcaaagcagt
     2701 acgatccaaa ttttgacata catccgttcc atcttgatct gttaagcctg agtattgaat
     2761 ctgagtctag ctgtttattc ggataattca tcggttgaac acaaagtacc gctgcaacgt
     2821 atagttgtac agaaccgcaa cgctggtagc ggtgataact ttggcaagga tcggactaag
     2881 ccccgcgcgg ctgagcacat agattccggc ggcattgagc gcaaggtttc ccatccaaac
     2941 catgaggtaa cggttaacct gggccgccca tttcccttcc tgtgccctaa acacccagtt
     3001 ccggctgatc ataaaatgcg caactccgcc gcagagcgtg cctgtcgcgc cataaaaggt
     3061 cacccgtccc gttgccgccg gcgagggcag acccgcccac cgcagcaaca ggtagtacac
     3121 cagaaaatcg acgcccgttg ccaccagtga ggtgatctgt gctttcgtaa aggtgatcat
     3181 cgtgttttta caaaatggac tgcaacaacg acaagctgca gaaccagatt ggcataaatt
     3241 cccgccagca catcatccat cataacgccc cagccgcctt taagttcttc cattctccgg
     3301 acataaaagg gttttacgat atcgaaggcc cggaaaagca gcaaacccgt caagaggtac
     3361 ggccactgca cgggcacgaa aagcagggta aagctcattc cgacccattc gtcgatcacc
     3421 acgcggtagc tgtcctttcc ccaccccggt tcgacctttc cggaactata ggtacctaaa
     3481 gcaagcaatg aaagggtaat cagcaacggc ggccaaatat gatcatttcc gccaccgaag
     3541 gcaagatacc aggctataca gcaaacaaga gcagcaaccg tgcctccgcc cttcggtatg
     3601 taccctatcc ccaggcaagt ggaaatgagt cgatggatac gcaccgtcat gcggatattt
     3661 cttcctcggc ataggtaagg ccgtccggat ggacgagttc ctcacccatc agccgccgca
     3721 atgcattttg caaggtcgtc agctgcttga atatgtcgtg ctcaggcctg cagcccggcg
     3781 catgttgcgg tgatttgaaa taaaaggaca accattcctg tacgccgctg agacccgccc
     3841 gcttggcgag atcgccgaac aatgcaaggt ccaggacgag aggcgctgcg aggatcgaat
     3901 cgcggcagag gaaattgatc ttgatctgca tggagtaacc cagccagccg aagatgtcga
     3961 tattgtccca actctctttg ttgtccccgt gcggcggata gtaattgata cggatcttgt
     4021 ggtagagctc gccgtagagc tcgggagtga gatcggggcg aaggatgtct tccagcacgc
     4081 tcagcttcga aacttccttt gtccggaaat tatcggggtc gtccagcaca tacccgtcac
     4141 ggttacccag gatgttcgtc gagaaccagc cttttacccc gagtgcgcgc gcctgaaggc
     4201 cgggtgcgag gatcgttttc atgagcgtct ggccggtttt gaaatcttta cctcctatgg
     4261 ggctttctgt ccgtttggcc agttcgacca gcgccggaat gtccacggtg aggttgggtg
     4321 cgccgttgag gaaggggacg cccgaccgaa gcgccgcata cgcatagatc atactgggcg
     4381 cgatcctcgg atcgttctct ttcagccctt tttcaaatgc gctgatggtc ttgtgcacat
     4441 cggccacttc gatgtatttt tccgtagatc cgcaccagac catcacaag
//
LOCUS       DATGKE010000545         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_922462, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000545 DATGKE010000000
VERSION     DATGKE010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922462"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VKU83_09305"
     CDS             complement(<1..897)
                     /locus_tag="VKU83_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI93795.1"
                     /translation="MTTTRRQFIRQGALAGAATYIGAMGAPASSYRRIIGANDRVNVG
                     VVGFSDRHRNDHIPAFLQSRKEMNFDIIAVSDIWKKRREEGKAFLAEKFGHDIQACVN
                     NEELYTIKAVDAVMISTADFQHALHTIEAVKAGRDTYTEKPLAETMADARAVFDAVKE
                     SGKIVQIGSQRRSGANYQAAAQFIQSGKFGPIVMVELTWNVNQPGRWRRPALVSQLRE
                     EDTDWNRWLLNRPKEVFDPRKYLEFRLFWPYSSGLPGQWMSHQIDTVHWFSGLPHPRS
                     ASANGGIYAWKDGRRNADTMTVV"
     gene            982..2043
                     /locus_tag="VKU83_09310"
     CDS             982..2043
                     /locus_tag="VKU83_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HLI93796.1"
                     /translation="MISFMSRNGLRAVLALLAFPAPLFSAAQRLPVQIVADSINRSVI
                     VTAGGIPFTTFWYPDTLEKPVLYPIYAPDGEVITRGFPIKPRPGEPADHPHHVGLWFN
                     YENVNGLDFWNNSYAIPPARKGQYGWIRTDSVSIRSGGPDDASISYAARWTDQQDHTL
                     LTERTHFRFSATAHERIIDRVTRLTAQTDVQFPDAKDGMLGLRVTRELQIPSNAPGEF
                     IDDKGNITSVPAGNAPDINGNYLTSAGRTGDSAWGTRGNWCMMYGKKGADTISIVIID
                     HPSNPGYPTYWHARGYGLLAANPFGWREFHNDPDKDGSWTIQQDKQLKFRYRVLIHPG
                     DYQRAKVAQAYQEYAAHER"
BASE COUNT          416 a          675 c          556 g          427 t
ORIGIN      
        1 gacgaccgtc atcgtatccg cattcctgcg tccgtccttc cacgcataga taccgccgtt
       61 agccgatgcc gaccgggggt gggggaggcc cgagaaccaa tgcaccgtgt cgatctggtg
      121 gctcatccac tgtcccggca gcccggaaga gtacggccag aacagccgga actccaggta
      181 ttttctcgga tcaaaaacct ccttcggcct attcagcagc caccggttcc aatccgtatc
      241 ctcctcgcgg agctgcgaca ccagggccgg ccgccgccat cgccccggct gattcacatt
      301 ccacgtgagc tccaccatga cgatagggcc gaatttcccc gactggataa attgcgccgc
      361 ggcctggtaa ttggcgccgc tgcgccgctg cgagccgatc tgaacgatct tccccgactc
      421 cttcaccgcg tcgaacaccg cacgggcgtc cgccatggtc tcggcgagcg gcttttccgt
      481 gtaggtgtcg cggccggcct ttacggcctc tatcgtatgc aaggcatgct ggaagtcggc
      541 ggtgctgatc atcaccgcgt ccactgcctt gatcgtatac agctcctcgt tattcacaca
      601 ggcttggatg tcatggccga atttctccgc caggaatgcc ttcccctcct cccttcgctt
      661 tttccagata tccgacaccg cgatgatgtc aaaattcatc tccttgcggg attgcaaaaa
      721 ggccgggata tgatcgttcc ggtgccggtc ggagaatcct acgaccccga cattaacccg
      781 gtcgttcgcg ccgatgatgc ggcgatagct gctcgccggt gcgcccatgg cgccgatgta
      841 cgtggctgcg ccggccagcg cgccctgtcg gataaattgc ctgcgggtcg tcgtcatatg
      901 gaatgaattt ttatggtgat gataccagta cccccgataa atgtactaat ttacatcttc
      961 ccacacgtca acccttccag tatgatctca ttcatgtcac gcaatggtct gcgggccgtc
     1021 ctcgccctgc tggcgttccc cgccccgctg ttctctgccg cgcagcggct gccggtccag
     1081 atcgtcgccg actccatcaa ccgcagcgtt atcgttacgg caggagggat acctttcacg
     1141 acattctggt atcccgacac gctcgagaaa ccagtcctgt accctattta tgcgcccgat
     1201 ggcgaggtga tcacccgggg cttccccatt aagccgcggc cgggtgaacc cgccgaccac
     1261 cctcaccacg tcggcctgtg gttcaattac gagaatgtca acggtctcga tttctggaac
     1321 aattcctacg ctatcccgcc ggccaggaaa ggccagtatg gctggatccg taccgattcg
     1381 gtcagcatcc ggtcaggcgg cccggacgac gcgagcatta gctacgccgc ccgctggacc
     1441 gaccagcagg atcatacctt gctgaccgaa cgcacccatt tccggtttag cgccaccgcg
     1501 catgaacgga tcatcgaccg tgtcacccgg ctcaccgccc aaaccgacgt tcaatttccc
     1561 gacgcaaagg atgggatgct ggggctgcgc gtcaccaggg aattgcagat cccgtccaat
     1621 gctcccggtg aatttatcga tgacaaaggc aatatcacta gcgttcccgc gggtaacgcg
     1681 cccgatatca acgggaatta cctcacttcc gccggccgga ccggggacag cgcctggggc
     1741 acgcgcggca actggtgcat gatgtacggc aagaaaggcg cggatacgat cagcatcgtt
     1801 atcatcgacc atccttccaa tcccgggtat cctacctact ggcatgcgcg cggctacgga
     1861 ctgctggcgg caaatccttt cggctggcga gagttccata acgatccgga caaagatgga
     1921 agttggacga ttcaacagga caaacagttg aagttccgtt accgcgttct catccatccc
     1981 ggcgactacc agcgggccaa agtcgcacaa gcttaccagg agtacgcagc ccatgaacgg
     2041 tagataagcc ttgggaaagg gcctgtgctt caag
//
LOCUS       DATGKE010000546         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1193259, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000546 DATGKE010000000
VERSION     DATGKE010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193259"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VKU83_09315"
     CDS             <1..292
                     /locus_tag="VKU83_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HLI93797.1"
                     /translation="VSLCKEFYSLQSQRNPTDPGESVAYPLKDAGNSAEMLLECRFCL
                     TQYHPAYGDPAAGVAAGTPIDRLDPGYVCPVCGAPRADLLPVEKMVPVSGQS"
     gene            304..1266
                     /locus_tag="VKU83_09320"
     CDS             304..1266
                     /locus_tag="VKU83_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLI93798.1"
                     /translation="MEPIRDQYGRTFRTLRVSLLSQCNFGCVYCVAGENELREVNNAG
                     RQEMLPTARLLEMIGRLHEQLVLETIRLTGGEPLLYPGLTTLVAGIHALGIEKIKLTT
                     NGALLDRKAAELTKAGLRSVNVSLDAVEEGAFARMSRRNALERVVAGIDAALAAGLTV
                     KLNAVIMKGINEDQILPLLDFARQRGITVRFLEVMAMGHLFHHSAPYLFAQREILGVI
                     GEHYALNALERKPSSTARYWETDAGHRFGIIANESEPFCGDCDRLRLDSGGNIYGCLS
                     SNHPIALDPKDDTGQLTEKLRQALGQKQAVKFTGSELSMLKIGG"
     gene            1286..1519
                     /locus_tag="VKU83_09325"
     CDS             1286..1519
                     /locus_tag="VKU83_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93799.1"
                     /translation="MKIHVYAGLKEYFLPEFELADEVGNIDQLKACLLAINERAAMLL
                     AACRFAVEEGFIDNNYKFKSHETVIVVPPSSGG"
     gene            1479..2003
                     /locus_tag="VKU83_09330"
     CDS             1479..2003
                     /locus_tag="VKU83_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLI93800.1"
                     /translation="MKQSSSFRPAAAGSMAVKDAPIDMAEMLAGAHHPGAGAVVLFSG
                     EVRGENKGRAVAFLEYEAHVPLATAMMGEILSEAKQKWGLTFAVAQHRVGRLDVGDTA
                     VVVITASAHRHEAYAANRHIIDRIKHEAPIWKCEHFVDGSSEWGNNCNCHEVTGDYSK
                     HVYSRPEAGSFPAQ"
     gene            2006..2566
                     /locus_tag="VKU83_09335"
     CDS             2006..2566
                     /locus_tag="VKU83_09335"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HLI93801.1"
                     /translation="MDPGAQSLIGVVLCGGESRRMGRDKGLIRSEGLCWAVRMGRKLL
                     PWGLPVVYSIRAEQEASYSAVLPEAYLLADRMGLGGPLNGLHSVHGSWPRADLLLLGC
                     DLQDMDEETIGDLIAAYRAGSAECYIYRDGTFAQPLCGIYTGKGLQRVLAQVPAERSL
                     QGLIRRMHESSLQIKRAGAFANYNSL"
     gene            complement(2632..>3428)
                     /locus_tag="VKU83_09340"
     CDS             complement(2632..>3428)
                     /locus_tag="VKU83_09340"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HLI93802.1"
                     /translation="IKGKGKLTMTFTPDDGGAAQTFEVFHFTGDGVALSMYNTDESIK
                     GFARSCFNMALNKKWPLYLSTKNTILKKYDGRFKDIFEEIYEQEFKAKFKEAGIVYEH
                     RLIDDMVASALKWNGNFVWACKNYDGDVQSDSIAQGFGSLGLMTSVLITPDGKIMEAE
                     AAHGTVTRHYRDHQAGKPTSTNPIASIFAWTRGLAFRGKLDNNQPLIDFANALEAVCI
                     ETVESGKMTKDLAICIHGNKVEHGRDFLYTEEFLDAIDAGLQKKLK"
BASE COUNT          714 a          876 c         1082 g          756 t
ORIGIN      
        1 cgtatcgctt tgcaaggagt tctatagcct ccaaagccag cgaaatccaa ccgacccggg
       61 ggaaagcgtc gcttaccccc tgaaggacgc cgggaattcc gcggaaatgc tgttggaatg
      121 caggttctgc ctgacgcaat accatcccgc ttatggggat cccgctgcgg gggtcgcggc
      181 ggggacgccc atcgaccgtc ttgatcctgg ctatgtctgc ccggtttgcg gagcgccccg
      241 ggccgatctg ttgcctgttg aaaaaatggt tcctgtcagc ggacagtcgt aactttatca
      301 ccgatggaac caatcagaga tcagtatggg aggacattcc ggaccttgcg ggtcagtttg
      361 ctcagccaat gtaactttgg ctgtgtgtat tgcgtcgcag gagagaatga gctacgcgaa
      421 gtgaacaacg ccgggcggca ggagatgcta cccacggcca ggctgctgga aatgatcggg
      481 cgccttcacg aacagctggt cctggagacg atccggttaa ccggtggcga gccgcttctt
      541 tatccggggc tgacgacgct cgtggccggt atccacgcat tggggatcga aaaaataaaa
      601 ctgacgacga atggcgcgct tctggatcga aaggccgcag agctgacaaa ggcggggctg
      661 cgctccgtga acgtgtcgct ggatgcggtg gaggaagggg catttgcccg catgagccgg
      721 cgtaatgccc tggagcgcgt cgttgccggg atcgatgcgg cgcttgcggc cggcctcacg
      781 gtaaagctta acgccgttat catgaagggg atcaacgagg accagatatt acccctgctg
      841 gacttcgccc gtcagcgcgg gatcaccgta cgatttttgg aggtgatggc catgggtcat
      901 ctttttcatc actccgcgcc ttatctcttt gcccagcgcg agatcctggg cgtcatcggg
      961 gagcactatg cgctgaatgc gctggagcgg aaaccttctt caacggcccg gtactgggaa
     1021 acggacgccg gacatcgctt cgggatcatc gccaatgaga gtgaaccgtt ttgcggtgac
     1081 tgcgaccggc tgcggctcga cagcggcggg aacatctacg gttgtctcag cagcaatcat
     1141 ccgatcgcgc tcgacccgaa agacgacacg gggcaattga cggagaaact gcggcaagcc
     1201 cttgggcaga agcaggcggt gaaattcacc ggcagcgagc tgagtatgtt aaagattggc
     1261 ggataaaaca agaaaaaggc agattatgaa aattcatgtc tatgcggggc tcaaggaata
     1321 tttcctgccg gaattcgagc tggcggacga ggtgggcaat atcgaccagc tgaaggcctg
     1381 cctgctcgcg atcaacgagc gggcggccat gttactggca gcgtgccggt ttgctgtcga
     1441 agagggtttt attgacaata actacaaatt caaatcgcat gaaacagtca tcgtcgttcc
     1501 gcccagcagc ggcgggtagc atggcggtaa aggatgcgcc gatagacatg gcggaaatgc
     1561 ttgccggcgc tcatcatcct ggtgcgggcg cagtggtatt gttcagtggg gaggtgaggg
     1621 gagaaaataa gggcagggcg gtggcattcc tggaatatga agctcatgta ccgctggcga
     1681 cggcaatgat gggggagatc ctttcggagg ccaagcaaaa atggggcttg acctttgccg
     1741 ttgcacagca ccgggtaggg cggctcgacg tcggagacac ggcggttgtg gtgattacgg
     1801 catcggcgca tcgtcatgag gcctatgctg caaaccggca cattatcgac cggatcaaac
     1861 acgaggcgcc gatttggaaa tgcgagcatt tcgtggatgg atcttccgag tggggcaata
     1921 attgcaattg tcatgaggtg accggcgatt attccaaaca tgtatatagc cggccggagg
     1981 ccggttcgtt tcccgcacaa taatcatgga cccgggcgct cagtcattga ttggggtggt
     2041 actctgcggc ggcgagagcc gccggatggg gcgtgacaaa gggttgatcc ggagcgaggg
     2101 actatgttgg gcggtacgga tgggccggaa gctgctccca tgggggctgc cggtggttta
     2161 ttcgatccgg gcggagcagg aggcttctta ctcggcagtt cttcccgagg cttacctgct
     2221 cgcggaccgg atggggttgg ggggacccct gaacgggttg cacagcgtgc atggctcgtg
     2281 gccgcgggcg gatctgctgc tgctgggctg cgatcttcag gatatggatg aggagacgat
     2341 cggcgacctg attgccgcat atcgtgccgg gagtgctgaa tgttatattt accgggacgg
     2401 aacatttgcc caaccgcttt gcgggattta tacgggcaag ggattgcagc gggtcctggc
     2461 gcaggtcccg gcggagcgca gcttgcaggg attgatccgg aggatgcacg aatccagtct
     2521 gcagattaag cgcgccgggg cgttcgcgaa ttacaattct ctttgaacaa actaccggaa
     2581 taaaaagccc ccgcgtgtac ggcgggggct ttagcgcgct tactgcgtgt attatttcag
     2641 tttcttctgc aaaccggcgt cgatggcgtc gaggaattcc tcggtgtaca ggaagtcccg
     2701 cccatgttcg accttgttgc catggatgca gatggcaagg tccttggtca tcttgccgct
     2761 ttcgaccgtc tcgatgcaga cggcttcgag tgcgttcgcg aagtcaatca gcggctggtt
     2821 attgtccagc ttccctcgaa aggccagccc acgggtccac gcaaatatgc tggcgatggg
     2881 gttggtcgaa gtcggcttgc ccgcctggtg atcgcgataa tgccgtgtga cggtgccgtg
     2941 ggccgcttcc gcttccatga tctttccgtc cggcgtgatg agcacggagg tcatcaaacc
     3001 aagggaaccg aatccctggg cgatgctgtc actctggacg tccccgtcgt agtttttgca
     3061 ggcccagaca aaatttccgt tccatttcag ggcgctggcg accatatcat cgataaggcg
     3121 gtgttcatag acgataccgg cttccttaaa tttggccttg aattcctgct catagatctc
     3181 ctcgaagata tctttgaagc ggccgtcata ttttttaagg atggtattct tggtcgacag
     3241 atacagcggc cattttttat tcagggccat gttaaagcaa ctgcgcgcga accctttgat
     3301 cgactcatcg gtattataca tgctcaatgc cacgccatcg cctgtgaaat ggaagacctc
     3361 aaacgtttgg gcggcgccgc cgtcatcggg cgtaaacgtc atggtcagtt ttcctttgcc
     3421 ttttatta
//
LOCUS       DATGKE010000547         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_110147, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000547 DATGKE010000000
VERSION     DATGKE010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110147"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..>2078
                     /locus_tag="VKU83_09345"
     CDS             217..>2078
                     /locus_tag="VKU83_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLI93803.1"
                     /translation="MKKCLLLLLLALVLFRVNAQDQTVSGKVTDEAGKPLAGATVTIK
                     GTNLSATTDENGNFHINTGNSTRPVFTISYVGFESFDYAYKGRSGFSVQLHPDTRALG
                     DVVVIGYGVQRKRDVTGATATVKAEQIVKKPVVRVEEALQGTVSGVSVQSVNGMPGQG
                     LAVRIRGTASINNNNDPLYVIDGQIGGDISTISPNDIESMEVLKDASATAIYGSRGST
                     GVVIITTKLGREGKPRIDINPWVKKEAILKELPLMNAYQFAVATNAQYTTTGSPAAFT
                     DAQIQGFKTNPGTNWQKSLQDKPWVQNYQVSVSGGSNDVKYMVSYLHLDQPGLIINQW
                     YRTDIARANFLIKASDRLNLQFNLAGSIPNNRNTQYQGDITDPFAEAYAWDPTSPIKD
                     ANGNFILSSSHASLGINPISQQTNGLNDNTGANYNATGILTWHILKGLTFTSNNTYAN
                     SSNINRQLYGPGTATYRDNPANGYALQNSSYGHSFQNSNYFTYKGTWGDHDLTVTALY
                     EQSSGTSYNNQSRATNLSTYSLGYWNLGLGANQNTSSGYSSQALQSYMGRAEYKFMDK
                     YLVNATVRDDGSSVLTQKYSVFPSGAVGWIVSKENFLASSRVISFLKLRASYGVT"
BASE COUNT          513 a          573 c          549 g          443 t
ORIGIN      
        1 cccttggcat gggcaagctg gctaccgtag gactggctgc tgccgtatat ggtcctacca
       61 tgaatatctc tggttcggca ctcgcgacgt ggtggcaccg cagattgcct gctgaagaaa
      121 atattgggaa gaaattggct attaatcagc cggttaatcg ataattaaag ggcctgttaa
      181 cccttgcagc gtttttttgt atcaaaactt gcttgtatga aaaaatgtct gctactgctt
      241 ctgctagcct tggtcctgtt ccgggtaaat gctcaggacc aaacggtcag cggcaaagtg
      301 accgacgaag ccggcaaacc gctggctggt gcaacggtca ctattaaagg caccaatctc
      361 agtgccacca cggatgagaa cggcaacttc cacatcaata ccggtaatag tacaaggccg
      421 gtcttcacta tctcttatgt aggctttgaa agttttgact atgcctataa aggccgttca
      481 ggattctctg tgcagcttca tcccgacacc cgggcgttgg gcgatgtcgt agtcatcggc
      541 tacggcgttc agcgcaagcg ggatgtcacg ggcgctactg cgacggtcaa agccgaacag
      601 atcgtcaaaa agccggtagt gcgggtcgaa gaagcgctcc agggaacggt ctcgggcgtc
      661 agtgtgcaga gtgtcaacgg catgccgggt caggggctgg cggtccggat acggggtacc
      721 gcgtccatca acaacaacaa cgacccgctc tatgtgatcg acgggcagat cggcggcgat
      781 atctccacca tctcgcccaa tgacatcgaa tccatggaag tgctcaagga tgcctccgcc
      841 accgccattt atggttcgcg gggttctacc ggggtcgtga tcatcaccac taaattaggc
      901 cgcgagggca agccccggat cgatatcaat ccgtgggtta aaaaagaggc gatcttaaag
      961 gagctgccgc tgatgaatgc ctatcagttc gcggtagcta ccaatgcgca atacaccact
     1021 accggttcgc ccgccgcttt tacagacgcg cagatccagg gtttcaagac caatccgggt
     1081 accaactggc aaaaatcgct gcaggacaaa ccctgggtac agaattacca ggtgtcggta
     1141 tcgggcggca gcaacgacgt aaaatacatg gtgtcctatc tgcatctcga tcagccgggg
     1201 cttatcatca atcaatggta ccgtaccgac atcgcgcgcg ccaacttcct gatcaaggcc
     1261 agcgaccggc tcaatctgca attcaacctg gcaggttcta ttcccaacaa ccgcaacacg
     1321 caatatcagg gggatatcac cgatccgttt gccgaggcgt atgcctggga cccgacatcg
     1381 ccgataaagg atgccaacgg caattttatc ctgtcgtcgt cgcatgcctc gctcggcatc
     1441 aatcccatct cgcaacagac caacgggctg aatgacaaca ccggcgccaa ttataacgcg
     1501 acgggcatcc tgacctggca tatcctcaag gggttgacct ttacgtcgaa caatacgtac
     1561 gccaacagct cgaacatcaa ccggcagctg tatggccccg gcactgccac gtatcgggat
     1621 aatccggcca atggctatgc cttgcagaat tcgtcctacg gacatagctt ccagaacagt
     1681 aactacttta cttataaagg gacatggggc gatcatgatc ttaccgtcac cgcactttac
     1741 gaacagtcga gcggaacgag ctacaacaat cagagcaggg ctaccaatct gtccacttac
     1801 tcactgggtt attggaacct tgggctgggg gcgaaccaaa acaccagctc gggctattca
     1861 tcacaagcgt tgcaaagtta tatggggaga gcggaataca agttcatgga caaatatctg
     1921 gtgaatgcca cggtccggga cgacggtagt tcggtcttga cgcagaaata cagcgtgttc
     1981 ccctccggag ctgtcggctg gatcgtgtcg aaggagaact tcctggccag ctcgcgggtc
     2041 atctcctttc tgaagctgcg cgccagctac ggggtaac
//
LOCUS       DATGKE010000548         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_345084, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000548 DATGKE010000000
VERSION     DATGKE010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345084"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VKU83_09350"
     CDS             complement(<1..1001)
                     /locus_tag="VKU83_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93804.1"
                     /translation="MAGSSNDTTFINPANTGFPAYLDFTTMRSNAINYLGPITGKYWT
                     DYNVHDPGITTLEVLIYALMDLGYRVNLPIGNLLAGSTFFTPGQVFGCNPTSVIDYRK
                     LLMDLDEVRNAWLEPDGSAINGLYVVYLETEKDESDFSTAGQWEKYKHAVHTKVRDIL
                     HAYRNLCEDIGRIVFLRKVYIGVNADIEIVPGTSISDTYQAVVQALYSFFSPVPAFYT
                     LAQLTAMNISLDTVFEGRPYTGRPSHGFLLDGDMPVRPSGEQTIYRSAVIQTILGVSG
                     IKTVRSLALLDAQKHPLNGGATWVVRFGDDVIPAFSLAASTFRWYQNGQQLSVQVNS"
     gene            complement(985..>2596)
                     /locus_tag="VKU83_09355"
     CDS             complement(985..>2596)
                     /locus_tag="VKU83_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93805.1"
                     /translation="QVGSCPGREDYPYSFSIRPEDFGLSGQLSYDPAFAPLTNYSSSV
                     LNGFLRVTMEDQDFLHKVYPIVMAEKVIARAHHHNVALPNEPWTPTVLEIAIDYEAQA
                     GHADMQLIQLYPYNGTYKEVDIDGEPTLFATFCDEGNLFIGLSGLVPGDGLNMLFAFA
                     EATAETEGGAVTIDWYYLAANQWVPLRTGFEVIADATNGLSTTGIVQYHFPDDVSEDN
                     TILPAGSYWVRASAAGGAAATSQTRAIVTQAVLASFVNNTTLNDQTRPGNTPLKAGSI
                     SKLAVPDPNIASVTQPYDSFGGHAPEALNNVYTLRVSEHLRHKGRAIQKWDYERMVLQ
                     AFPKILRAKCINHSYALSSLDYRWDFPMAPGNILIAVLPDPAQLAVTDALQPTVPMSM
                     LTGIETWLAGYVSPFVRLFVRNARYEPIDMCLRVALQPGLNGSFYQSQLAQDIRHFMA
                     PWQDGNTDVFQFGQRLYSVDLVQFIEGLDYVTNLLDLEMCHAGDAMPDPAPDYMEPLT
                     PRSILVAGKIVVRIQGASKKTIANGWKQ"
BASE COUNT          579 a          767 c          697 g          553 t
ORIGIN      
        1 taggaattca cttgaacact gagctgctgc ccgttctggt accagcggaa cgtcgatgcg
       61 gcaagcgaaa aggcagggat cacgtcatcg ccaaaacgaa ccacccatgt cgcgccgccg
      121 ttcagcggat gtttctgcgc atccagcaac gccagcgatc gcaccgtttt gatgccggaa
      181 acgccaagta tcgtctggat gacggccgac cggtaaatag tttgttcgcc cgaaggccgg
      241 accggcatgt ccccgtcgag cagaaaacca tgactgggac ggcccgtgta gggtcggcct
      301 tcgaataccg tatccagcga tatattcatg gccgtaagct gcgccagcgt ataaaaggcc
      361 ggcaccggcg agaaaaaact atacaatgcc tgtaccaccg cctgataggt atcgcttatg
      421 ctggtccccg gcacgatctc gatgtccgcg ttgaccccaa tatagacctt tcttaaaaat
      481 acgatccgcc cgatatcctc gcataggttc cgataggcat gcaaaatatc ccgcactttc
      541 gtatgcacgg catgcttata tttctcccat tgcccggcgg tggaaaaatc gctttcatcc
      601 ttctcggtct cgagatagac cacatagagc ccgttgatcg ccgacccatc gggctccagc
      661 cacgcattgc gtacttcgtc gagatccatc agcagtttcc ggtaatcgat caccgaggtc
      721 ggattacagc cgaatacctg ccccggtgta aagaaggtac tgccggccaa caaattacct
      781 atcggcagat tcacccggta ccccagatcc atcagggcat agatcagcac ctccagcgtc
      841 gtgatccccg ggtcatggac gttgtaatcc gtccaatact ttccggtgat ggggcccagg
      901 taattgatgg cattcgaccg catcgtcgta aaatccagat atgccgggaa ccccgtattg
      961 gccggattga taaaagtggt atcgttactg cttccagcca tttgctattg tcttttttga
     1021 tgcgccctgt atccggacaa cgatcttccc cgccaccagt atcgaccggg gcgtcagcgg
     1081 ctccatataa tccggcgccg gatcgggcat ggcatcgccc gcgtggcaca tctccagatc
     1141 gagcaggttc gtcacataat ccaggccttc gatgaattgc accagatcga cgctgtagag
     1201 ccgttgcccg aactgaaaca catcggtatt accatcctgc cacggcgcca tgaaatggcg
     1261 gatgtcctgc gccagctgcg attgataaaa gctgccattc aaaccgggtt gcagcgcgac
     1321 ccgcaaacac atatcgatcg gctcatagcg tgcattccgg acgaacagcc gcacaaaagg
     1381 cgaaacatat cccgcaagcc aggtctcgat cccggtcagc atgctcatcg gtaccgtggg
     1441 ctgcaacgca tcggtgaccg ccagctgcgc gggatcgggc agaacggcta taaggatatt
     1501 ccccggcgcc atcggaaaat cccaccggta atccaggctg ctcagcgcat agctatggtt
     1561 aatgcatttt gcccgaagta tcttggggaa cgcctgcagt accatccgtt cataatccca
     1621 tttctggatg gccctcccct tatgccgcag gtgttcgctg acccgcagcg tataaacatt
     1681 gtttaatgcc tcgggcgcgt ggccgccaaa cgaatcataa ggctgggtca cgcttgctat
     1741 attcgggtcc gggacggcca gcttggagat ggacccggct ttgaggggag tattcccggg
     1801 tctggtctgg tcgttgagcg tcgtattgtt gacaaagctg gccagaacgg cctgcgtgac
     1861 gatggccctg gtctggctcg tcgcggccgc gccgccggcg gcggatgccc gtacccaata
     1921 gcttccggcg ggaagaatgg tattgtcttc actcacatcg tcgggaaaat gatattgaac
     1981 gatcccggtg gtcgacagtc cattggtggc atcggcaatg acctcgaaac ccgttcgcag
     2041 cggcacccat tgattggcgg cgagataata ccaatcgatc gttacggcgc cgccttccgt
     2101 ctccgcagtt gcttcggcaa acgcaaatag catgttcagc ccatcgccgg gcaccagacc
     2161 cgataagccg atgaacagat taccttcgtc gcaaaaggtg gcaaaaagcg tgggctcgcc
     2221 atcgatgtcc acttctttat aggtgccgtt atacggatac agctggatca gctgcatatc
     2281 ggcatgtcct gcctgcgcct cataatcgat ggcgatctcc agtaccgtag gggtccaggg
     2341 ctcgttgggc agcgcgacat tgtggtgatg cgcccgcgcg atgaccttct cggccatcac
     2401 gatgggatac accttatgca ggaaatcctg atcctccatc gtcacccgga gaaagccatt
     2461 cagcacgctg ctgctataat tggtcagggg cgcgaatgca ggatcgtagc taagctgacc
     2521 gctcagaccg aaatcttccg gccggatgga aaaggagtag ggatagtcct ccctgccggg
     2581 acagctgccg acctgc
//
LOCUS       DATGKE010000549         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1427645, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000549 DATGKE010000000
VERSION     DATGKE010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427645"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VKU83_09360"
     CDS             complement(<1..1056)
                     /locus_tag="VKU83_09360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93806.1"
                     /translation="MPTVIGLVSYPFLPARVGGQRGVALFYKYFSRWCPVVCVTTRKN
                     DPAAAEGYAVLNILSNSKLRYINPRYYFVLRRIIREYQAGVLILDHPYYGWLGVLLRW
                     TCPIRLVIRSHNLEGRRWRTQGKWWWWILWLYERWVHRRADLSTFIQEEDKAYAIRRY
                     ALKPERCTTITYGLERDHAPSSGEVARSREIVRSSLGLAEDETLLFFNGAFDHAPNRE
                     ALEYIVREINPLLQQMNGFRYRILICGKGIPEAISKAGHPNMLFAGYVEDIDRYFRGA
                     DVFINPVITGGGIKTKLVEALGNNLTAVSVRSGATGIDPAWTNGKLLVVEDGDWPGFV
                     RLIAAAREVRTDIPPVYF"
     gene            complement(1058..>2198)
                     /locus_tag="VKU83_09365"
     CDS             complement(1058..>2198)
                     /locus_tag="VKU83_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93807.1"
                     /translation="TADYQTAHDSDLKERFIQAKMQQLSQTHQAGPDAQQQAVSTANA
                     LISALRSDRQSIYGGDLLRTIVLMALAAAVLGLYIRGKIKPVILLAGLIVLTGYDLLT
                     VSNRYLSEDNYTEASEIETAISPTPADQQIRNDPDKNFRVFDQSSQSPFEDSRASYFH
                     NSIGGYSPAKLGLYQDLIDNQLLKGNMQVYDMLNTKYFIGRDPKTGQSVAQQNPGAFG
                     PTWLVKGIRFVANGDQEMKALDSVDLRDTVVIQQRYSSMVKTAPVADSTAHIRLQENL
                     NDKITYRSSARTPQFAVFSEIYYPEGWDAYIDGVKVEYLRVDYLLRGLSVPAGDHTIE
                     FRFEPRSFAKADAIAFSSSLAVCLLLLTAGVVEWRRNRPKRSNPA"
BASE COUNT          472 a          638 c          610 g          478 t
ORIGIN      
        1 gaaataaaca ggcgggatat ccgtcctcac ctcccgtgcc gctgcgataa gccggacgaa
       61 tccgggccag tccccatcct caacgaccag cagcttgcca ttggtccacg cgggatcgat
      121 gcccgtcgct ccgctgcgta ccgacacggc cgtcaggtta ttgcccaggg cctctactaa
      181 tttcgttttg atgcctccgc ccgtgatgac cgggttgata aaaacatccg caccccgaaa
      241 gtaacgatcg atatcctcta catagccggc gaaaagcata ttcggatgac cggccttgct
      301 gatcgcttcc ggaataccct ttccacagat gagtatcctg tatcggaaac cattcatctg
      361 ctgcaaaagc gggttgattt cccgcacgat atattcgagt gcttcccggt tcggcgcatg
      421 gtcgaaagcc ccgttgaaga acagcagcgt ctcgtcttcg gccagaccaa ggctgctgcg
      481 tacaatttcc cggctgcgcg ccacttcccc ggatgacggt gcgtggtccc tttccagccc
      541 ataggtaatc gttgtgcatc gttccggttt cagcgcgtac cggcggatcg cataagcttt
      601 gtcctcctcc tggataaagg tgctgagatc ggcccggcga tgcacccatc gttcgtagag
      661 ccataggatc caccaccacc atttgccttg tgtcctccaa cgccggcctt cgagattatg
      721 ggatcggatg accagccgga tggggcaggt ccagcgcagc agcacgccca accagccata
      781 ataaggatga tccaggatga gtacacccgc ctgatactcc cggatgatcc tgcggagcac
      841 gaaataatac cggggattta tgtaacgaag ctttgagttg gacaggatat tcagcaccgc
      901 atagccttcg gcagcagcag gatcattctt ccgcgtggtt acgcatacga ccgggcacca
      961 ccgcgagaaa tatttgtaga agagcgcgac ccccctttga ccgccgaccc tggcgggtag
     1021 aaagggataa gagaccagac cgataacagt tggcatacta tgcggggttg ctgcgtttgg
     1081 gccggttccg tctccattcc acaactccgg ccgtaaggag cagcagacat accgccagac
     1141 tggagctgaa agcaatggca tcggccttgg caaaggaccg cggctcgaaa cggaactcga
     1201 tggtatggtc gccggcagga acggacaagc cgcggagcag ataatccacc cgaagatatt
     1261 ccactttcac cccatcgata taagcatccc aaccttccgg ataatagatc tcgctgaata
     1321 cggcgaactg cggcgttctg gcggacgacc ggtaggtgat cttgtcattc agattctcct
     1381 gaagccggat atgtgcggtg ctatcggcta ccggcgcagt cttgaccatc gacgaataac
     1441 gctgttggat gaccacggta tcccggagat cgacgctgtc cagcgctttc atttcctgat
     1501 cgccgttcgc tacgaaccgg atgcctttca ccagccaggt cggaccgaaa gcacccggat
     1561 tttgctgcgc cacggattgc cccgtcttgg gatcacgacc aatgaaatat tttgtgttca
     1621 gcatgtcata gacctgcatg tttcccttca agagctggtt atcgatgagg tcctgataca
     1681 ggcccagctt agcgggtgaa tagccgccga tcgagttgtg gaagtaggag gcgcgggaat
     1741 cttcgaacgg gctttgcgag gactggtcga agaccctgaa attcttatcg ggatcgttgc
     1801 ggatctgctg gtcggccggc gtcggactga tggcggtttc gatctccgat gcttccgtat
     1861 agttgtcctc gctcagatat cggttactca ccgtgagcag gtcataaccg gtcaggacga
     1921 tcagacctgc cagcaggatc accggtttga tcttgccccg gatatacaag ccgaggacgg
     1981 ctgcggccaa cgccattaaa acaatggtcc gcagcaggtc gccgccatag atcgactggc
     2041 gatcggaccg cagggcggag atcagcgcat tcgccgtcga taccgcttgc tgctgcgcgt
     2101 ccggtccggc ctgatgtgtc tgcgacagct gctgcatctt ggcttggatg aatcgttcct
     2161 tgagatcgct gtcatgcgcg gtctggtagt ccgcagtg
//
LOCUS       DATGKE010000550         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_479004, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000550 DATGKE010000000
VERSION     DATGKE010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479004"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2752)
                     /gene="mfd"
                     /locus_tag="VKU83_09370"
     CDS             complement(<1..>2752)
                     /gene="mfd"
                     /locus_tag="VKU83_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLI93808.1"
                     /translation="HSPRLFELVNSILLSAPQRISCKNLQGSSPAFLISAIFQQDSTS
                     QLNHLVVCEDSEAAAYVHNTIESLTGALDLFYFPSSFKNKKNYRLLNSSHVMLRTEAL
                     TRLSSARGTNKKLIVTYPEALFEKVVLPEALSENIISIKTGDTLDLNGVLGKLVDKGF
                     DRTDFVYEPGQFALRGGILDIYSFGNEKPYRVELFGNDVDSIRIFDPETQLSERKLLQ
                     VNIIPNVENRPDGGVKISLLEFLPDNTVVWMQDWSFARERLTIQEEDLTLFLDHIRNA
                     PPAKGPAVDADEEKLEKADVTPDQFVTAADIETQLEKFHLIEFGPSAASAFPNPVVIA
                     FHTRPQPSFNRQFELLIKDLKALEAKKFTLYLFAENPRQLERLHTIFEDLRSAVAFTP
                     VALSIHEGFIDEDLKIACYTDHQIFQRYHKYKVKQAYNKNKAITLRTLRELQPGDYVT
                     HIDHGVGIYSGLQKIEANGRLQEAVRIIYKDSDILYVNINSLHKISKYTGKEGTVPKV
                     NKLGSDAWQKLKEKTKTRVKEIAFDLIKLYAQRKAEKGFAHSPDNYMQTELEASFIYE
                     DTPDQSKATADVKKDMELPSPMDRLVCGDVGFGKTEVAIRAAFKTCCDSKQAAILVPT
                     TILAFQHFKTFGDRLKDFPVKVDYVNRFKSAKDKKETLKRLERGEVDIIIGTHALLGK
                     EVKFKDLGLLVIDEEQKFGVAHKEKIKTLRTHVDCLTLTATPIPRTLQFSLMGARDLS
                     IINTPPPNRQPIQTELHAYNEDFIRDAIYYETERGGQVFFIYNRVQGLAEMAALLQGL
                     CPDLSIGYAHGQMEGHELEDRILSFIDRKYDVLVCTNIVESGVDIPNVNTIIVNNAHH
                     FGLSDLHQLRGRVGRGNRKAFCYLIAPSLATLPDDSRKRLQTLEQHSDLGSGFQIAMR
                     DLD"
BASE COUNT          589 a          689 c          760 g          714 t
ORIGIN      
        1 tatcgaggtc gcgcatggcg atctgaaaac cgctcccgag gtcgctgtgc tgttcgagag
       61 tctgaagccg tttgcgcgag tcgtcgggca gcgtagccag ggatggggcg atcagatagc
      121 agaaggcctt tctgttgccc ctgcctaccc ggccccgcag ctggtgcaga tcgctcaatc
      181 cgaaatggtg cgcattattg acgattatcg tattgacatt cggaatgtcc acgccgctct
      241 ccacgatgtt ggtacatacc aggacgtcgt attttctatc gatgaaagag aggatacggt
      301 cctccagctc atgtccctcc atctgtccat gtgcatagcc gatacttaga tcggggcata
      361 gcccctgtaa aagcgcggcc atctcggcca gaccttgcac ccggttatag ataaagaaca
      421 cctggccgcc ccgttccgtt tcgtaataaa tggcgtcacg gataaaatct tcattgtaag
      481 catgcagctc cgtttggatc ggctggcggt ttggtggcgg agtattgata atactcaggt
      541 cgcgggctcc catcaggctg aactgaagcg tacggggtat cggcgttgcg gtcagggtca
      601 gacaatccac atgtgttcgc agggtcttga tcttctcttt atgggccacg ccgaactttt
      661 gctcctcatc gataaccaac agcccaaggt ctttgaattt tacttccttt cccagcagag
      721 catgtgttcc tatgatgata tcgacctctc cccgctccaa ccgctttagg gtctcttttt
      781 tatcctttgc ggatttgaac cggttcacat aatccacttt tacgggaaaa tccttgagcc
      841 ggtcgccgaa ggtcttgaaa tgttgaaagg ccaggatggt ggtcggcaca agtatggccg
      901 cctgtttgct gtcgcagcag gtcttgaatg ccgctctgat cgctacctcc gttttgccga
      961 aacctacatc gccgcagacc aggcggtcca tgggtgaagg cagctccata tcttttttta
     1021 catcggccgt ggccttgctt tgatcgggcg tatcctcata gatgaacgag gcttccagct
     1081 ccgtttgcat ataattgtcg ggggaatggg caaatccttt ttccgccttg cgctgcgcat
     1141 aaagcttgat gaggtcgaag gcgatctctt taacacgggt cttggtcttt tccttgagct
     1201 tttgccaagc atcgctaccc agcttattta ccttgggtac ggtaccttct ttgcccgtat
     1261 atttcgagat cttatgaagt gagttgatgt ttacatacag gatatcgcta tccttgtaga
     1321 tgatccggac ggcttcctgc agacggccgt tggcttctat cttttgaagg ccgctataga
     1381 tgcctacgcc atgatcgata tgcgtcacat agtcccccgg ctgcagctcg cgaagagttc
     1441 gaagcgtaat cgccttgttc ttgttgtagg cctgctttac cttatactta tggtaacgct
     1501 ggaagatctg atggtcggta tagcaggcta ttttgagatc ttcgtcgata aatccttcat
     1561 ggatcgatag ggccacggga gtaaaagcga cagccgaccg gaggtcttcg aaaatggtgt
     1621 gcagacgttc cagctggcgc gggttctcgg caaaaagata aagtgtgaac tttttggctt
     1681 ccaatgcctt gagatccttg atgagcagct cgaactggcg gttgaaagat ggttgaggcc
     1741 gggtatggaa ggcgatgacg accggattgg ggaaagcgga tgcggcagat ggcccgaact
     1801 cgataagatg aaacttttcc agctgcgttt cgatgtccgc ggccgtgacg aattgatcgg
     1861 gtgttacgtc cgccttttcc agcttttctt cgtcagcgtc cacggcggga ccttttgcag
     1921 gcggcgcgtt gcggatatga tcgaggaaca aggtaaggtc ttcttcctga atggtaagcc
     1981 gctcccgggc aaaggaccaa tcctgcatcc aaacgaccgt gttatccggc aggaattcca
     2041 acagagagat cttcaccccg ccgtcaggcc ggttttcgac attaggtatt atattgactt
     2101 gcaataactt gcgttcactg agctgcgttt cgggatcgaa gatgcggata gagtccacgt
     2161 cattgccgaa cagctctacc cgatagggct tttcattgcc gaacgaatag atgtccagga
     2221 tgccgccgcg caaagcgaat tgcccgggct catatacaaa gtccgtgcgg tcgaatccct
     2281 tgtcgaccag ctttcccaat acaccattga ggtcgagcgt gtcgccggtt ttgatggaga
     2341 tgatattctc cgacaacgct tccggcagca cgaccttttc gaaaagcgct tccggataag
     2401 tgacgatcag ctttttattg gttcccctgg ccgaggaaag ccgggtaagc gcttcggttc
     2461 gcagcatgac atggctgctg ttgagcaggc ggtaattctt cttattctta aaggaagagg
     2521 ggaaatagaa aaggtccagc gcgccggtca ggctttcgat ggtattgtgc acgtaggcgg
     2581 cggcttccga atcctcgcag acgacgaggt gattgagctg gctggtgcta tcctgttgaa
     2641 atatagcgct gatcaggaag gccggggaac tgccctgaag gttcttgcag gagatccgtt
     2701 ggggagcgga caataggatg ctgttcacca gctcgaaaag gcggggagaa tg
//
LOCUS       DATGKE010000551         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_935872, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000551 DATGKE010000000
VERSION     DATGKE010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935872"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VKU83_09375"
     CDS             complement(<1..1385)
                     /locus_tag="VKU83_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HLI93809.1"
                     /translation="MKSKKKKKNKPSHHHSSRLYKGTLDVTRSGMGFVMVEGRDQDIL
                     VRPADFNTALHGDTVQVRVTSEPGRSGRLTGEIAEVVERKQLEFLGHIEISQSFAFFI
                     AEMDKPMPDIYVPLSQLNGAANNDKVIVRITEWEKNKKPQGTVIQVMDASDENDRSMK
                     EILLENGFPIFFPENVMEEAGRLPDLIPTAEIARRKDLREILTFTIDPLDARDFDDAI
                     SIRTLESGQLEIGVHIADVSHYVEPDTALDREAYDRATSVYLPDRVNPMLPERISNEL
                     CSLRPNEDKLTFSAIFRMTPKAEIKHRWIGRTVIHSKHRFTYEEVQEIIEQGEGPYHA
                     EIAALHALAQRFRRHRFKKGAINFSSTEVRFKLDPRGKPIGIMVKESKESHQLVEEFM
                     LLANREVAEYVGKITIHKKPVPFPYRVHDEPDKEKLLPFIEFAKKYGHQFDTKTPQSV
                     AASFNQMLQDV"
     gene            complement(1375..>2051)
                     /locus_tag="VKU83_09380"
     CDS             complement(1375..>2051)
                     /locus_tag="VKU83_09380"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HLI93810.1"
                     /translation="RPGTRVVILDDAFQHRSVRAGLNILLTDYSNLFTRDWWLPTGDL
                     RDSPSSYRRADIIVVTKCPEELTVGESREIGAEIKPLAHQRVFFTAIRYGEPYHITRR
                     TNAVIDGRVEVLLVTGIANPAPLKRWLDERSRTYYELAYSDHHIFSIDDLRTIIRRFE
                     SITAPDKLILTTEKDAERLLKFRHELEAWPFYVLPIAPRFLFDEEPGFTDLIVTFITE
                     FKKNEE"
BASE COUNT          418 a          548 c          591 g          494 t
ORIGIN      
        1 tggacatcct gcagcatctg gttgaacgaa gcggcgacgg attggggcgt cttcgtatcg
       61 aattggtggc catatttttt ggcgaattcg ataaacggca gcagcttctc tttgtccggt
      121 tcatcgtgga cacggtaagg aaagggcacg ggctttttat gaatagtgat ctttcccacg
      181 tattccgcga cctcgcggtt ggccagcagc ataaactctt caaccagctg atgcgactcc
      241 ttgctttcct ttaccatgat tccgatcggt ttcccccgcg ggtccagctt gaaccgcact
      301 tcagtagagg agaaattgat cgcgcctttt ttgaaccggt ggcggcgaaa ccgctgggcg
      361 agggcatgga gcgcggcaat ttcggcatgg taaggcccct ctccctgttc gatgatctcc
      421 tgcacttctt cataggtgaa ccggtgtttg gaatggatga ccgtccggcc gatccagcga
      481 tgcttgattt ccgccttcgg cgtcatcctg aagatcgccg aaaaggtcag cttgtcctcg
      541 ttgggccgca aggaacaaag ctcgttggaa atgcgttccg gcaacatcgg attgacccgg
      601 tcgggaaggt agacggacgt agcccggtca tacgcttccc tgtcgagggc cgtatccggt
      661 tcgacataat ggctgacgtc ggcgatatgg acaccgatct ccaattggcc ggactccagg
      721 gtccggatgg aaatagcatc gtcgaagtcc cgggcatcga gcggatcgat cgtaaacgtg
      781 agaatttcgc gcagatcttt ccgccgggca atctcggcag tggggataag gtcagggagg
      841 cgcccggctt cttccattac attttccggg aaaaagatgg ggaagccatt ctcgagcagg
      901 atctccttca tgctccggtc gttttcgtcg ctggcgtcca tgacctggat gaccgtgccc
      961 tgtggtttct tgttcttttc ccattccgtg atccgcacga tcaccttgtc attattggcg
     1021 gccccgttca gttgtgacag cggaacatag atgtccggca ttggtttatc catttccgcg
     1081 ataaagaagg cgaacgactg gctgatctcg atatgcccga gaaactcgag ctgtttgcgt
     1141 tccaccactt ccgcgatctc gccggtaagg cggccgctgc ggccgggctc gctggtcacc
     1201 ctgacttgca cggtgtcgcc atgcagggcc gtattgaagt cggcgggcct gacgaggata
     1261 tcctggtcgc ggccttcgac catcacaaag cccattccgg agcgggtgac gtccagcgtg
     1321 cctttgtaca gccgggacga atgatgatgg gatggtttat tctttttctt tttcttactc
     1381 ttcattcttc ttgaattctg tgatgaatgt aacgattaaa tcagtaaagc ccggttcctc
     1441 gtcgaataaa aaccggggcg cgataggcag gacatagaac ggccaggctt cgagttcgtg
     1501 ccggaatttc agcagccgtt cggcgtcctt ttcagtcgtc agaataagct tatcgggcgc
     1561 cgtaatgctt tcgaaccggc ggataatcgt tctcagatca tcgatgctga agatatgatg
     1621 atcactgtaa gccagttcgt aataagtccg ggaccgttcg tcgagccatc gcttcagggg
     1681 cgccgggttc gcaatacccg ttacgagcag tacttccacc cgaccgtcga tcacggcatt
     1741 ggtcctgcgg gtgatatgat agggttcacc gtaccggata gccgtaaaaa aaacccgttg
     1801 atgcgccaaa ggcttgattt cggcgccaat ttcccggctt tcgccgaccg tgagctcctc
     1861 cgggcatttg gtcacgacaa tgatatcggc ccggcgatag ctggaggggg aatcgcgcag
     1921 atcgccggtc ggcagccacc agtcgcgggt gaaaaggtta gaataatcgg tcaacagaat
     1981 gttaagccca gccctgacgg aacggtgctg gaaggcatcg tccaggatga cgacacgggt
     2041 gccgggccgg t
//
LOCUS       DATGKE010000552         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1090146, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000552 DATGKE010000000
VERSION     DATGKE010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090146"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1430)
                     /locus_tag="VKU83_09385"
     CDS             complement(81..1430)
                     /locus_tag="VKU83_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLI93811.1"
                     /translation="MKRALIVIVLLLAAGGAVWYFFFNKETQPVVLSTEEPKYGYISK
                     SVTATGTIEPVDTVNVGCQVSGTINGIYTDYNAKVKKHQLIASLDKSLFEATVNQYKA
                     NLQLAKSTLVYAQNNFERESTLYKAGAISKADYDNYENQYLGAKATVGSVQAQLDAAQ
                     KNLDYANIYSPVDGFVMTRNVSVGQTVAASFNTPTLFMIAKDITKMQVQAAVSEADIG
                     DVRPGLRATFTVDAYPDISFTGTVSQIRLEPVVSANVVTYTTIISAPNQDLKLRPGMT
                     ANIFIFTKEVDSALLVSAKALKFKPDPTMEKQFRIIPDSIGEREVQRAARRETNNPLA
                     PPRHHPDTTGRQVDTASTEGTPAFVWVQVGDSLVERLVTTGLNNDTQVQILSGLITGD
                     VVVNGTGIVTKGTKPGATARSPFMPTRRPSGSSRGNTRGGAAGGAGGGAGGRGNGGR"
     gene            complement(1507..2847)
                     /locus_tag="VKU83_09390"
     CDS             complement(1507..2847)
                     /locus_tag="VKU83_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93812.1"
                     /translation="MFLRLSVSLFLGLGMLTGDVDAQDTASGGLPAHWDLQTCIDYAK
                     KNNITLNSLRLSVRTAEQNYLLAKAARQPTLGGDVTANYTHSKNANPVIGGFQTQSSF
                     ANNYSLSSGVTIYNGGAISDNIRMANLQIQSANQTVVQGELDITLQIVQEYLNILLAK
                     ENVKYVEDLVNSTTAQVAQGKQEYEVGTIARNAYIELQANLAADKYNLVTAQNAVRQN
                     KLTLKQLLQLPLRDSFDIVTPDTVVSSQAVPSLDSVEAYALRNRPEIQLGLLDIDISH
                     YNLAIAKTGYLPLLTASATLSSGYSNNQKDDFVQQVDNNFFQQAGLNLSVPIFNRRQV
                     KTNVENAKIQTEQAKLTLLGNQTALSQTIEQAYINVLNAQGQYDAAVQELSANQEAFR
                     VAGEELKVGASNLVNYLQQKTLYTQAFENYIQAKYNSALSIEIYDFYMGIAVKL"
     gene            3019..3597
                     /locus_tag="VKU83_09395"
     CDS             3019..3597
                     /locus_tag="VKU83_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HLI93813.1"
                     /translation="MGTLTGKKVAMLTENGFEEVELTSPKRALESAGAEVQIISPQKE
                     KVRAWDKDHWSIELPVDEVLDRAKPEEYDALVLPGGVLNPDQLRQNKKAVEFAQQFLQ
                     AGKPVAAICHGPQLLIETGMLEGRMMTSYWSLKTDLTNAGVQWVDKEVVVDHGLVTSR
                     SPKDLEAFNRKAIEEIREGQHSPVAHTPSKAE"
     gene            3653..>4130
                     /locus_tag="VKU83_09400"
     CDS             3653..>4130
                     /locus_tag="VKU83_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HLI93814.1"
                     /translation="MPEGPSIVILKEQVKHFAGKKVLAADGNTKVGKERLKGLTVRTF
                     KSWGKHFLICFPRLTVRIHFLLFGSYSINEQTKPNPRLHLKFATGDLYFYACSVKLLE
                     ASPDELYDWTADVMNPAWDPAAARRKLKASPGMLVCDALLNQEIFAGVGNIIKNEVL"
BASE COUNT          827 a         1089 c         1253 g          961 t
ORIGIN      
        1 tccgaaccca tttggaactc acgccggata tcatgcagtt ccagtatttt gtgatcgttc
       61 atggtcattt caatttacgc ctaccggccg ccgttgccgc ggcctcctgc gccgcctccc
      121 gcgcctcccg cggctccgcc gcgggtgttg cccctgctgc ttccggacgg tcttcgggta
      181 ggcatgaacg gacttcttgc cgtggcgcct ggtttggttc ccttcgtaac gatcccggtc
      241 ccgttcacca ccacatcgcc cgttattagc cccgaaagga tctgcacctg ggtgtcgttg
      301 ttcagcccgg ttgtgaccag ccgctctacg aggctatccc cgacctgcac ccacacgaac
      361 gctggggtgc cttcggtcga ggccgtatct acctggcgac cggtcgtatc gggatggtga
      421 cgcggagggg cgagcgggtt attggtctcg cggcgcgcgg cccgctgaac ctccctttcg
      481 ccgatgctgt cgggaatgat cctgaattgt ttctccatcg tcggatcggg cttgaactta
      541 agcgccttcg cggaaaccag caaagcgctg tcgacctcct tggtgaagat gaagatgttc
      601 gccgtcatcc ccggcctgag tttcaggtcc tggttcggag cgctgataat ggtcgtatac
      661 gtcacgacat tcgccgagac tacgggttcg aggcggatct ggctgacggt gccggtaaat
      721 gaaatgtcag ggtacgcgtc gacggtgaaa gtggcgcgca acccggggcg cacgtccccg
      781 atgtcggctt ccgatacagc cgcctgtacc tgcatcttgg tgatatcctt ggcgatcatg
      841 aacagcgtcg gggtattgaa gctcgcggcg acggtctggc ctacgctgac attgcgggtc
      901 atgacgaagc cgtccacggg cgagtagata ttggcgtagt caaggttttt ttgcgcggcg
      961 tccagctgcg cctggacgct gcctaccgtc gccttggcgc cgaggtattg gttctcgtag
     1021 ttgtcgtaat cagccttgct gatcgcgcct gccttgtaca gcgtgctctc ccgttcaaag
     1081 ttattctggg catagacgag cgtagatttt gctagctgca gattggcttt gtactggttg
     1141 accgttgcct cgaaaaggga tttgtcgagg gaagcgatga gctggtgctt cttcactttc
     1201 gcgttgtagt cggtataaat gccattgatc gtccccgata cctggcagcc gacattcacg
     1261 gtatccaccg gctcgatggt cccggttgcc gtaacgcttt tcgagatata gccgtatttg
     1321 ggctcttccg tgctgagaac gacgggctgg gtttctttat taaaaaagaa ataccagacg
     1381 gcgcctccgg cggcgagaag taggacaatg acgatgagtg ctcgcttcat ataatttttt
     1441 tgtctaagga tccatcaggg tctgcataag gttgctccgg ccggacccgg ccggagcctg
     1501 ctccgctcat aatttgacgg cgatgcccat atagaaatcg tagatctcga tactgagcgc
     1561 cgaattgtat tttgcctgga tataattctc gaatgcctgg gtgtacagcg tcttttgctg
     1621 gaggtagttt accaggttgg atgccccgac cttcagttcc tcgcccgcga cccggaaagc
     1681 ttcctggttg gcactcagtt cctgcacggc ggcgtcgtac tgaccttgcg cgttcagcac
     1741 gttgatatag gcctgctcga tggtctgcga cagggcagtc tgattgccga gcagggtgag
     1801 cttggcctgt tccgtctgga tctttgcgtt ctcgacattg gtctttacct gtctccggtt
     1861 gaagatcggg accgacaggt tgagcccggc ctgctgaaaa aaattgttgt ccacctgctg
     1921 gacgaaatcg tctttttggt tattggaata tccgctggat aatgtcgcgg acgcggtgag
     1981 cagggggaga taaccggttt tggcgatagc caggttatag tgcgagatat cgatgtccag
     2041 caggcccagt tggatctccg gcctgttccg cagggcgtag gcttcgaccg aatcgaggga
     2101 cgggacggcc tgcgaagata ccacggtgtc gggcgtcacg atgtcgaagc tgtcgcggag
     2161 gggcagctgc agcagttgtt tcagcgtcag cttgttctgc cgcacggcgt tctgcgcggt
     2221 caccaggttg tacttgtcgg cggcgaggtt cgcctggagc tcaatatagg cgttcctcgc
     2281 gatagtgccg acctcatact cctgcttgcc ctgcgccacc tgggccgtag tcgaattcac
     2341 aaggtcctcg acgtatttta cgttctcttt ggcaagcagg atgttcagat actcctgcac
     2401 gatctgcagg gtgatatcga gctcgccctg gaccacggtc tggttcgcgc tttggatctg
     2461 gaggttggcc atccggatat tgtcggagat cgcgccgccg ttatagatcg taacgcccga
     2521 ggacaacgaa tagttattgg caaacgaaga ctgggtctga aatccgccga tcaccgggtt
     2581 ggcattcttg ctgtgcgtat aattggcagt gacatcaccc ccgagcgtgg gttgccgggc
     2641 ggcctttgcg aggagataat tctgttcagc cgtccggacc gagagccgca ggctgttgag
     2701 cgtgatgttg ttcttcttcg cataatcgat acaggtctgg aggtcccagt gcgccggcag
     2761 gccgccggaa gcggtgtcct gggcatccac gtcacctgtc agcatgccca ggccgaggaa
     2821 cagcgagacc gataagcgta agaacataaa gccctggttt agtttgatgt gatgcagatg
     2881 tggtacaaag gccaggccat accgtgagct tattcaccca cccgtgtaaa tgtaggcatt
     2941 tattaggttc gcggtccggg ttgaagcgcg cgcggtggta ttctttttga agctgttgtc
     3001 cgtataaaaa acgagtatat gggaacatta acgggaaaga aggtcgcgat gctgaccgag
     3061 aacggtttcg aggaggtgga gttgaccagc cctaaaaggg cgctggagtc ggcaggcgcg
     3121 gaagtgcaga tcatttcgcc gcaaaaggaa aaggttcgtg cgtgggacaa agaccattgg
     3181 tcgatcgaac ttccggtcga cgaggtcctt gaccgggcga aaccggaaga gtacgatgca
     3241 ttggtccttc cgggcggcgt gctcaacccc gaccaattgc gccagaataa gaaggctgtg
     3301 gaatttgcgc aacaattcct gcaggcagga aaaccggttg cggctatctg tcacggaccg
     3361 cagctgctga tcgaaacggg catgctcgaa gggcgaatga tgacgtccta ttggtcattg
     3421 aagaccgatc tgacgaatgc gggcgtacaa tgggtggata aggaggtggt ggtcgatcac
     3481 gggctcgtga ccagcaggtc gcccaaggac ctggaggcat tcaacaggaa agcgatcgaa
     3541 gagatcaggg aaggtcagca ttcgccggtc gctcatacgc cttccaaggc ggaatagcgg
     3601 aacagcggaa cagcggggtc cgccatttgc gcttcgatcg ataaaacaag ccatgcccga
     3661 agggccttct atcgtcatat tgaaggagca ggtgaagcat ttcgccggga aaaaagtgct
     3721 cgccgccgat ggcaacacga aggtcggcaa agagcggctg aaaggcctga cggttcggac
     3781 cttcaaaagc tggggcaaac acttcctgat ctgctttccg cggctgacgg tacggatcca
     3841 tttcctgctt tttgggtctt acagcatcaa tgagcagaca aaaccgaatc cacggctgca
     3901 cctgaagttc gcaaccggtg acctgtactt ttatgcctgc tcggtaaaac ttctcgaggc
     3961 atcgccggac gaactgtacg actggacggc cgatgtcatg aacccggcat gggatccggc
     4021 cgcagcaaga aggaagctga aggcgagtcc cgggatgctg gtttgcgacg cgctgctcaa
     4081 ccaggagatt tttgccggcg ttggaaatat tatcaagaac gaggtcctgt
//
LOCUS       DATGKE010000553         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1332372, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000553 DATGKE010000000
VERSION     DATGKE010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332372"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VKU83_09405"
     CDS             complement(<1..222)
                     /locus_tag="VKU83_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93815.1"
                     /translation="MSFIIDQQTLDDLDILSKQPGASIYLLFNRTFTRGGAAVLEEMF
                     RFPLTDPTRIRKRGAIIAYFSATTLFPFDT"
     gene            complement(224..2527)
                     /locus_tag="VKU83_09410"
     CDS             complement(224..2527)
                     /locus_tag="VKU83_09410"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HLI93816.1"
                     /translation="MKTTWRIAKLELNTLFYSPIAWFLSIVFLFQCGLAYIERIDHVL
                     TNQNLGGQYLQYINFSTANVFGPPAGMFMFILSKVYLYLPLITMGLISREVSSGTIKL
                     LYSSPVKVSQVVYGKFVAMMGYNLLLVLILAIFGITGIINIQHADSGLIFSGLLGIYL
                     LLCAYAAIGLFMSCLTSYQVVAAISTLVVFAMLDYVRTLWQGVDFVRNLTYFLSISGR
                     TSHMLAGLIETKDILYFLIVVIMFLAFSMIRLQSGRASWSGAGITARYVLVFVLALAT
                     GYVSALPRFVGYFDATTDNMETLTPNTQKVIREIGNQPLEVTSYINLLDRRYWYGQPT
                     ARNEDVERWEPYLRFKPNISFKYIYYYDTPYDRYDNLQRQYPGESLTQIAKNFARSFK
                     VDLSDFKTPEEIRKIVNLSPEQNRYVMQLKFRGKTTFLRLYDDSYTFPHESETTAALK
                     RLTVKLPKIAFATGEYERDVDKLGSRDFGVLTNDIVFRYSLINQGFDIDTVSLNDQRI
                     PKDIAVLVIGDPRANFSPAALQEIQAYIADGGNLLIAGEPDKEAVLNPILRPLGVQMM
                     DGMLVQKSKDYSMDMVESLLTKAGAALSNDLEKDFQDSVGVNMIGAAGLTYSKNGPFT
                     VTPLLMTDSIDTWNKKGRVVLDSAAVVYSAGDGDNHHSIPTALALTRMINGKEQRIIV
                     TGDADFLTTAAKDRYPNTNPIFDTQLFRWFTYGQFPIDASRPPSKDNRVHLTLTGITD
                     LKILFLGVLPGLVLLFAAVFLVRRKRK"
     gene            complement(2536..>2770)
                     /locus_tag="VKU83_09415"
     CDS             complement(2536..>2770)
                     /locus_tag="VKU83_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93817.1"
                     /translation="LVQMNHLPAAGELLRISGVTKVELLNERQARIFFNGDQDVTERI
                     VEASVKNGWRLREINLDKSSIDEIFAQLSQKSK"
BASE COUNT          691 a          696 c          709 g          674 t
ORIGIN      
        1 cgtatcaaaa gggaatagcg tcgttgctga aaaataagcg ataatagcgc cgcgcttacg
       61 gatacgtgtg ggatccgtaa ggggaaagcg gaacatctcc tccagcaccg cggctccccc
      121 gcgcgtaaag gtccggttga acaaaagata gatggaggcg cccggctgtt tactcaggat
      181 atccaggtca tccagggttt gctgatcaat aataaaggac atcctatttt cttttgcgcc
      241 tgacaagaaa tacagcggca aacagcagga caagtccggg caacacgcct aaaaacaata
      301 ttttcaggtc tgttatgccg gttaaggtta gatggacgcg gttgtctttt gaaggcggcc
      361 ggctggcatc gatcggaaat tgaccgtaag tgaaccacct gaataattgc gtatcgaaaa
      421 tggggttggt gttcgggtac cggtccttcg ccgcggtggt caaaaagtct gcgtcacctg
      481 ttacaataat ccgctgctct ttcccattta tcattcttgt taatgccagc gctgtgggga
      541 tagaatgatg gttgtcgccg tcgccggccg aataaactac ggctgcggag tccagcacca
      601 cccttccctt cttgttccag gtgtcgatgc tatcggtcat taatagcggc gtaacggtaa
      661 agggaccatt tttactatag gtcaggccgg ctgcaccgat catgttcacg cccacactat
      721 cttgaaagtc cttctccagg tcattggaca atgcggcgcc tgccttggtt aaaagggact
      781 caaccatgtc catggagtaa tccttgcttt tttggaccag catgccatcc atcatctgca
      841 cacccaaagg acgtaggatc ggattgagta cggcttcctt atcgggctcg ccagcgatca
      901 gtaagttgcc gccgtcagca atatacgctt gtatctcctg gagcgccgcc ggtgaaaaat
      961 tggccctcgg atcgccgatg accagaacag caatatcctt cggtatgcgc tgatcattga
     1021 gcgatacggt atcgatgtcg aatccctggt ttattaggga atatctgaat acaatatcat
     1081 tggtaagtac gccaaaatcc cttgacccta atttatcgac gtcccgctca tattctcccg
     1141 tggcaaaagc gatctttggc agctttacgg tcagtcgctt cagtgccgcc gtagtctccg
     1201 actcatgcgg aaaggtgtac gaatcatcgt atagtcgcag gaatgtggtt ttgcccctga
     1261 acttgagttg catgacatac ctgttttgtt cggggctcag gttgacgatc ttcctgatct
     1321 cttcaggggt cttaaagtcg ctcaggtcta ctttgaagga cctggcgaaa ttcttggcaa
     1381 tctgggtaag actctcgccg ggatactgcc gttgcaggtt atcataccgg tcataggggg
     1441 tatcatagta ataaatatac ttgaaggaga tgtttggctt gaatcgcagg tatggctccc
     1501 agcgttccac gtcctcgtta cgcgccgttg gctgaccata ccagtaacgg cggtccagca
     1561 ggttgatata cgatgtcact tcaagcggct ggtttccgat ctcccggatc actttctggg
     1621 tattgggcgt aagcgtctcc atattgtccg tggtggcgtc aaaatacccg acaaaccggg
     1681 gcagcgcgct aacgtatccc gttgccagcg ccaagacaaa taccaggaca taccgggcgg
     1741 taatcccggc gcccgaccag cttgcccgtc ccgattgcag cctgatcatg ctgaaagcaa
     1801 ggaacatgat gacaacgatc agaaaataca atatgtcttt tgtctcgatc agccccgcca
     1861 gcatatggct tgtccggccc gaaatggaga ggaaataggt gaggttcctt acaaaatcaa
     1921 cgccctgcca aagcgttcgg acgtaatcaa gcatggcaaa aacaaccagt gtgctgatcg
     1981 cggcgacgac ctggtatgac gttaggcaag acataaagag ccctatggcc gcgtaggcgc
     2041 acagcaacag atatatcccc agtaaccctg aaaagatcaa accggaatcc gcgtgttgga
     2101 tgttgataat gcctgtaatg ccaaatatgg caaggatcag cacgagcaac aagttatagc
     2161 ccatcatggc gacaaacttt ccgtacacga cctggctgac tttgaccggt gaagaataaa
     2221 gcaacttgat cgtaccgctg ctgacctcac gactgatcag gcccatggtg atcagcggaa
     2281 gatacaggta cacttttgac aaaatgaaca tgaacatccc cgcggggggg ccgaatacat
     2341 tggcggttga aaagttgatg tattgaaggt actgcccacc gagattttgg ttcgtcagaa
     2401 cgtggtcaat ccgctcgata tacgctaaac cgcactggaa gagaaaaaca atggacagga
     2461 accaggcgat gggtgaataa aataaggtat tgagctccaa tttggcgatt ctccatgtcg
     2521 ttttcatcaa ctattttatt tggatttttg ggataactgg gcgaatattt cgtctatgga
     2581 gctcttgtcg aggttgattt ccctaagccg ccagccgttc ttaacgctgg cttccacgat
     2641 ccgctcggtg acgtcctggt cgccgttaaa aaatatccgc gcctgccgct cattcaggag
     2701 ttcgacctta gtcacgccgc tgatccgcag caattcgccg gcagccggga gatgattcat
     2761 ctgaacgagc
//
LOCUS       DATGKE010000554         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_340300, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000554 DATGKE010000000
VERSION     DATGKE010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340300"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VKU83_09420"
     CDS             <1..473
                     /locus_tag="VKU83_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLI93818.1"
                     /translation="TCDGYVMHGDTPATIGSRIRDMKERRERKGLAPMQYGVAAYCIV
                     RSSESEVRKEINRITDVRPGSPGYGNYQQWLAGTQLEQKVSLEDYSVSNRGLRSGLTG
                     TPAEVEDRVREFEKAGVDFFLLQCSPQLEEMERFAEALSLSRPSQQARPFPAHQ"
     gene            complement(426..1127)
                     /locus_tag="VKU83_09425"
     CDS             complement(426..1127)
                     /locus_tag="VKU83_09425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLI93819.1"
                     /translation="MEPVLRTAGDHWLAIGFDRAGDLAGEKRGYLLAKRSFDLFIATL
                     AITGILTWLLPLLAILIKIDSSGPVFFVQKRVGRNGRLFRCFKLRSMLTNDAADESPA
                     GGNDRRITRIGSWLRSTHLDELPQFWNVIGGSMSIIGPRPYMPSDCRRLAELVPDADL
                     PLRYSVRPGITGMAQSMGLHGATPSDRHAIVERWRWDLYYIRHAGFRLDMAILGDTLA
                     LLMSGERTRLLRRAR"
     gene            1257..2174
                     /locus_tag="VKU83_09430"
     CDS             1257..2174
                     /locus_tag="VKU83_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93820.1"
                     /translation="MQLRSSILKVVAYFDLFSYPLAMEDIVYFLDTDTDEYTIRKEIG
                     ELVHDGCLFRTGAYYSLRDDPALAEKRNRGRLHADEMFPVARKGAQLLYQFPFVRGVF
                     ISGSLSKRCADDKADIDYFIVTTAGRLWIARTLMHLFKKLTYMRGRQHRYCMNYYVDE
                     AAMEIREKNIFTATELLTLIPASGDGCVASFFLANEWTTRFYPRYGQRNRESMSTRGS
                     SLVKRACEKLLDNRLGDRLDDYLRNLTARRWKKKEDRGDLNLRGLAMSLQCGKHYARP
                     NPEVFQQMVLNRYQRKMKELAVRWGAVFS"
     gene            complement(2179..>2576)
                     /locus_tag="VKU83_09435"
     CDS             complement(2179..>2576)
                     /locus_tag="VKU83_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93821.1"
                     /translation="FAPLLRPGGQVSLTILSKFCLWETMMLFRGKWKTAFRRPLSGTR
                     GTRSRVEGYPFRCWYYSPASVIRGLGTGYDLLGVEGVCTLVPPSYIEGFAGKHPGAYR
                     ALRVLENKWKTKWPWRNIGDYFIISFRKN"
BASE COUNT          560 a          781 c          723 g          512 t
ORIGIN      
        1 cgacctgcga cggatacgtc atgcacggcg atacgccggc cacgatcggg agccgtatcc
       61 gcgacatgaa agagcggcgg gaaagaaagg gattggcgcc gatgcaatac ggcgtggccg
      121 catactgtat cgtccgcagc agcgaatcgg aagtcaggaa ggagatcaac cggatcaccg
      181 acgtccggcc cggcagcccg gggtatggca actatcagca atggctggcg gggacccagt
      241 tggaacagaa ggtttcgctc gaagactact ccgtttctaa ccgcggtttg cgttccggcc
      301 tgacaggcac ccctgccgag gtcgaagacc gggtccggga attcgaaaaa gccggagtgg
      361 acttcttttt attgcaatgc agcccgcaac tggaagaaat ggagcgcttc gccgaggccc
      421 tttccttatc tcgcccttct caacaggcgc gtcctttccc cgctcatcaa tagcgcaagg
      481 gtatcgccta ggatcgccat atccagccgg aagccggcat gccggatgta gtatagatcc
      541 cagcgccacc gttcaacgat ggcatgcctg tcgctgggcg tggcgccatg aaggcccatc
      601 gattgcgcca ttcctgttat ccccggcctc acggagtacc gcaatggaag gtcagcatcc
      661 gggaccagct ccgccaaccg ccggcaatcg gaaggcatat acggccgtgg accgataatg
      721 ctcatcgaac cgccgatcac attccagaac tggggcagct catcgaggtg ggtgctgcga
      781 agccacgacc cgatgcgcgt tatccggcga tcgtttccgc cggcgggtga ctcgtcggcc
      841 gcgtcattgg tcagcattga ccgcaatttg aaacaacgga acaatctccc attcctgccg
      901 acgcgttttt gaacgaaaaa gacgggaccg ctcgaatcga ttttgatcag tatcgccaac
      961 agcggcagca gccaggtcag gattcctgtt atcgccagcg tggcgataaa aagatcaaaa
     1021 gagcgtttgg ccaggagata gccccgcttt tccccggcta aatcgccggc gcggtcaaaa
     1081 ccgatcgcca gccagtggtc gcccgctgtc ctgagtacag gttccatttt accgctcatt
     1141 ccatagcaaa cgagccgcgt tcccgaaagg ttgcccacct gcggctccgc gctgccggac
     1201 ccggtttggc gccgcgatca agcaactggt cgcttttatc ctgcgttcga tcttgtatgc
     1261 agcttcggtc gagcatattg aaggtcgttg cctattttga cctgttcagc tatcccctcg
     1321 caatggaaga catcgtttac ttccttgaca ccgacacgga cgaatacacg atccgcaagg
     1381 agatcggcga gctggtccat gacgggtgcc tgttccggac cggcgcctat tattcgttac
     1441 gggacgatcc ggcccttgcc gagaagcgga accgtggccg gctccatgct gacgaaatgt
     1501 tcccggtggc ccggaaaggt gcgcagctgc tttatcagtt cccattcgtg cgcggcgtct
     1561 tcatttcggg gtctttgtcc aaacgctgcg ctgacgataa ggccgacatc gattatttca
     1621 tcgttaccac cgccggcagg ctgtggatcg cccgcaccct gatgcatctc ttcaagaagc
     1681 tgacgtatat gcgggggcgc cagcaccgct attgcatgaa ctattatgtc gacgaggccg
     1741 caatggaaat aagggaaaag aacattttca cggctaccga actgctcacg ctgatcccgg
     1801 cgagcggcga cggctgcgtc gccagcttct tcctcgccaa cgaatggacc acccgttttt
     1861 atccacgcta cggccagcga aaccgcgaat ccatgagcac gcgcggatct tcgctggtca
     1921 aacgcgcctg tgagaagctg ctcgacaacc ggctgggtga ccgcctggac gattatctcc
     1981 gtaacctgac cgcacgccgc tggaagaaaa aagaggaccg cggggacctg aatttgcgcg
     2041 ggctggcgat gtcgctccaa tgcggcaagc actatgcccg gcccaatccc gaggtcttcc
     2101 agcaaatggt cctcaaccgt taccagcgta agatgaaaga gctggcggtc cgatgggggg
     2161 ctgttttttc ctgaaggctc aatttttcct gaaggaaatg ataaagtagt cgccaatatt
     2221 cctccagggc cattttgtct tccacttgtt ctccagcacc cgcaaagccc ggtaagcacc
     2281 gggatgtttt ccggcaaatc cttctatata ggaaggcggt accagggtac aaacgccttc
     2341 cacgccgagg agatcatatc cggtccccag gccgcggatg accgatgccg gcgaataata
     2401 ccagcacctg aaagggtacc cttcgacccg ggatcgcgtc ccccgcgttc cgctcaaagg
     2461 ccgccggaaa gcggtcttcc atttgcccct gaacagcatc atggtttccc ataggcaaaa
     2521 cttcgaaagg atcgtcaggg acacctgccc gccgggccgc agcagcggcg cgaaag
//
LOCUS       DATGKE010000555         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1273029, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000555 DATGKE010000000
VERSION     DATGKE010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273029"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VKU83_09440"
     CDS             <1..817
                     /locus_tag="VKU83_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HLI93822.1"
                     /translation="IANVTVKTVTRLLIDVGRACENFHNEKVMFVKADKIQCDEIWSF
                     CYSKQKNVPEGMEGVAGDIWTFTCLESESKLMISWLVGNRDAETTQMFMNDVAYRIKG
                     KPGQLSTDGMKEYQAAVSNAFGSHINYAQIVKQYSNPAEASTPERKYSPSKFIGAKRK
                     FVTGRPERKYISTSHVERSNLTLRMNSRRFTRLTNGFSKKIENHALAVALHMTYYNFV
                     RPHQSLRITPAMAAGLTKRFMTIEDIVRLVPEPVAKKRGPYKKTSKNIDAEKI"
     gene            932..2086
                     /locus_tag="VKU83_09445"
     CDS             932..2086
                     /locus_tag="VKU83_09445"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HLI93823.1"
                     /translation="MSTTTDFQIRVSRASRSRLQEVDWTNLELGASVSDHMFVCDFAD
                     GRWQAPEIAPFGPFTLSPTTLALHYGQTIFEGMKAFRTVDGRVHIFRPDRHYDRLVRT
                     TARMCMPAPPEELFMEGLRRLVALDKDWVPARPGTALYLRPMMFATGTKLTVRVSDNY
                     KLAIICLPAGAYFGKPLRVKVERHFVRAVRGGTGFAKCGGNYGGAFYPTQQAREEGYD
                     QVIWTDGVRHHFIEEMGMMNIFCVIAGVLVTPPLGDTLLDGVTRDSLLALARDGGLKV
                     EERAISVDELRNGLEKGIVTEAFGAGTAAVVAPIREIGIDSKDYALQVVGELESGTAQ
                     ARVARDGGDSAGLTIGAASGPSVAIWLKHQLEAIRYGRKPDKYGWNDYIS"
     gene            2156..2965
                     /locus_tag="VKU83_09450"
     CDS             2156..2965
                     /locus_tag="VKU83_09450"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93824.1"
                     /translation="MDQKLKGQVAIITGASSGIGEGVSKAMAAAGATVVVNHPVEATK
                     AAADAVLAQIKSAGGLGITYQCDVSNEGQVKKMFADVISQFGTVDILVNNAGLQRDAP
                     FLDMTLENWNYVLAVNLTGQFLCSKEAIREFLRRGIVPERSRAAGKIICMSSVHEVIP
                     WAGHANYAASKGGIMMFMKSIAQEFAPRKVRVNSICPGAIKTPINHSAWATPAAEKTL
                     MELIPYDRIGLPEDIGSVAVWLASDDSDYVTGASLFVDGGMTLYPGFSTNG"
     gene            3078..>4787
                     /locus_tag="VKU83_09455"
     CDS             3078..>4787
                     /locus_tag="VKU83_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93825.1"
                     /translation="MSIEKDRLKIPDWKKWGPYLSERQWGTVREDYSADGQAWTYTTH
                     DMAASKTWRWGEEGIGGFADKRLRICLAIGLWNKKDPIIKERLFGLNSHEGNHGEDVK
                     ELYYYLDSSPTHSYMKMLYKYPHAAFPYEQLLAENGRRTRQDPEYELLDTGIFDDDKY
                     FDVFIEYAKSDIDDILIRVTVHNRGSEAAPLHILPTIWFRNTWAWGYDDYKPTLVSSN
                     QGNITVTHRELGEFTLYGEEPDVHLFCDNETNRQRLYHSENPPFCKDGIQEYLIHGKE
                     DAINFEQGTKAALNFERLIPAGGSACVRLRLNANCPNGPFHQFDEIFASRIRETDEFY
                     AELQSEIADEDERRVQRQAFAGMLWSKQLYYYNVDEWLKGDPHQPPPPQERWTGRNHT
                     WTHLKNSNIISMPDKWEYPWYAAWDLGFHCLVFAMIDAEFAKHQLLLLTHDWYMHPNG
                     QLPAYEWSFGDTNPPVHAWAAYRVYEIDKANNGGTGDIAFLETVFHKLLLNFTWWVNR
                     KDEEGNNIFEGGFLGMDNIGLFDRSNTQLPDGSTLEQADGTSWMATYCLNLMRIALEL
                     SVTN"
BASE COUNT         1186 a         1198 c         1386 g         1017 t
ORIGIN      
        1 gatcgcaaat gttaccgtta agacggtcac acggctgctc attgatgtag ggcgtgcctg
       61 cgagaacttt cataacgaga aagttatgtt tgttaaggct gataaaatcc agtgtgatga
      121 gatttggtcc ttttgctaca gcaagcaaaa gaacgtcccc gaagggatgg aaggggttgc
      181 tggcgatatt tggactttta cttgtctgga atccgagagt aagctaatga ttagctggct
      241 cgttggaaat agggatgcgg aaactacgca aatgtttatg aatgatgttg cctaccgtat
      301 taaaggcaaa cctggacagc tttccactga tggcatgaaa gagtaccaag cggccgtttc
      361 aaacgcattt gggagccata ttaactatgc gcagatagtg aagcaataca gcaaccccgc
      421 agaagctagt accccggaaa ggaaatattc tccatcaaag ttcataggcg caaaaaggaa
      481 atttgtaacc ggccgcccgg aacggaaata cattagcacg tctcatgttg agcgtagcaa
      541 tttgaccctt agaatgaact cgcgccggtt tacgcgcctt accaatgggt tttccaaaaa
      601 gatcgaaaat cacgcgctgg cggtcgcctt acacatgact tattacaatt ttgttcggcc
      661 ccaccaaagc ctgagaatta ccccggcaat ggccgctggc ctgaccaaaa ggtttatgac
      721 aattgaggat attgttaggt tggtccccga acccgttgcg aagaaaagag gcccttataa
      781 aaagacctcc aagaatattg atgccgaaaa aatctaaatt tcaaatgacc acacgaccat
      841 agcaattaat ctgatctagt ccaaaataac aaatctgcat ctgttaaata tcggtcgata
      901 ttccgaaatt tacggcatta aaacaagtaa tatgtcaacg accaccgatt ttcaaatccg
      961 cgtcagccgc gcatcacgga gccggctgca ggaagtcgac tggacaaacc ttgagcttgg
     1021 cgcttcggtt tcagatcata tgttcgtttg tgattttgcg gacggccggt ggcaggcgcc
     1081 ggagatcgcg cctttcggcc ccttcacgct ttccccgaca accctggcgc tccactatgg
     1141 acagacgatc ttcgaaggca tgaaggcttt ccggacagta gacggccggg tccacatctt
     1201 tcggcctgac cggcattatg accggttggt tcgcacgaca gcgcggatgt gcatgcctgc
     1261 gccgccggaa gaactgttta tggaaggtct caggcggtta gtggcgctgg acaaggactg
     1321 ggtgccggct cggccgggta ccgctttgta tctgcggccc atgatgtttg caaccggtac
     1381 aaagttgacg gttcgggtat ccgacaatta taagcttgcc atcatttgcc tgcccgccgg
     1441 cgcctatttt ggcaagccgc tccgcgtgaa agttgagcgg catttcgtcc gtgcggtccg
     1501 cggcggcacc ggttttgcaa aatgcggcgg aaactatggg ggtgccttct atccgaccca
     1561 gcaggcgaga gaagaaggct acgatcaggt catctggacg gatggcgtgc ggcaccattt
     1621 cattgaagaa atgggaatga tgaacatatt ttgcgtgatc gccggcgtcc tcgtaacacc
     1681 gccactcggt gataccctgc tcgatggcgt aacgagggac agcttgttgg cccttgcgcg
     1741 tgacggcggg ctgaaggtcg aggagcgcgc gatcagcgtg gatgaattgc ggaatggttt
     1801 ggagaaggga atcgtgacgg aggcatttgg tgcaggaacg gcggcagtgg tcgcgccgat
     1861 cagggagatc gggatcgact cgaaggacta tgcactgcag gttgtcggtg aacttgaatc
     1921 gggaacggca caggcgcggg ttgcacgcga tggcggggat tcagcgggcc taacgatagg
     1981 cgctgcgtcc ggacccagcg ttgcgatatg gctgaagcat cagctcgagg cgattcgtta
     2041 tgggcgcaag ccggacaaat atggctggaa cgactatatc agttaagtaa aaaataactt
     2101 acctccttat tgtcccattg aattaatggc taaattgact gagaaatttt tagccatgga
     2161 tcaaaaactg aagggacagg tcgctattat tacgggcgcc agctccggca ttggagaggg
     2221 cgtatccaag gcaatggccg cagcgggagc aaccgtcgtc gtcaatcacc cggtcgaagc
     2281 cacaaaagct gcagccgatg ccgtgttggc tcagatcaag tcggcaggcg gccttggaat
     2341 cacttatcaa tgcgacgtca gcaacgaagg ccaggtcaaa aagatgtttg ccgatgtgat
     2401 ctcgcaattc ggcacggtgg atatccttgt gaataatgca ggtctccagc gggacgcacc
     2461 gttcctggac atgacattgg aaaattggaa ttatgtactg gccgtcaacc tgaccggcca
     2521 gtttctgtgc tcaaaggagg ccatcaggga attcctgcgc cgcggcattg tgcctgaacg
     2581 gtcccgggcc gcgggaaaga tcatctgcat gagcagcgtg cacgaggtga tcccctgggc
     2641 cggccatgcc aactatgccg ccagcaaggg cgggatcatg atgttcatga aaagtatcgc
     2701 gcaggaattt gctccccgca aggtccgggt caacagtatc tgcccgggtg cgatcaagac
     2761 cccgatcaac cattcagcct gggcaacgcc ggcggcggaa aagacgctga tggaactcat
     2821 cccctacgac cggatagggc tacccgaaga tatcgggtcc gtagccgtgt ggctggcgag
     2881 cgatgattcg gattatgtca ccggcgcctc attgtttgtg gacggaggca tgacattgta
     2941 cccggggttt agcaccaacg ggtgatcgtc gccgatcgtc gccgaccggc ggcgataggc
     3001 ggcgcaggat cgctacgggg aacggcggct aattccgctg gcatcccgcc aattgaaatg
     3061 tccattaact aagcgatatg agtatcgaaa aagaccggct gaaaataccc gactggaaaa
     3121 aatggggccc gtatctttcg gaacggcagt ggggcaccgt cagggaggac tatagcgcgg
     3181 acggccaggc ctggacgtat accacgcacg acatggcggc aagtaagacc tggcgatggg
     3241 gggaggaagg catcggcggg ttcgcggaca agcggctgcg gatttgtctt gcgatcggcc
     3301 tttggaataa aaaggatccg atcatcaagg aacggctgtt cggcctgaac agtcacgagg
     3361 gaaatcatgg cgaggatgtg aaggaactgt actattatct cgactcgtcg ccgacgcatt
     3421 cgtatatgaa aatgttgtat aagtatccgc atgcggcttt cccttatgaa cagctgcttg
     3481 cggagaacgg gcgccgaacc cgccaggacc cggaatatga actgctcgac acgggaatct
     3541 tcgacgacga caaatacttc gatgtcttta tagaatacgc caagtcggat atcgacgata
     3601 tcctgatccg ggtcacggta cacaaccggg gcagcgaggc agcgccgctc cacatcctgc
     3661 cgactatctg gttccgcaac acgtgggcat ggggatatga cgattacaag ccaacgctgg
     3721 tctcttccaa tcagggcaat ataacggtaa cgcaccgcga gcttggagag ttcaccttgt
     3781 atggcgagga gcccgatgtg catctgttct gcgataacga aaccaaccgt cagcggctct
     3841 accattcgga aaacccgccg ttttgcaagg acgggatcca ggagtacctg atccatggaa
     3901 aggaagatgc catcaacttc gagcagggga cgaaggcggc cctgaatttc gagcggctga
     3961 tcccggcagg cgggtctgcc tgcgtgcggt tgcggttaaa tgcgaattgt ccgaacggcc
     4021 cattccatca gttcgatgag atttttgcat cgcggatccg ggaaaccgat gaattttacg
     4081 ctgagctcca aagcgagatc gccgacgagg atgaacgacg cgtccaacgc caggcgtttg
     4141 caggaatgtt gtggagcaaa cagctgtact actacaatgt cgatgaatgg ttgaaaggcg
     4201 atccgcatca gccgcctccg ccgcaggagc gctggacggg caggaatcac acctggacgc
     4261 acctcaagaa cagcaacatc atttcaatgc cggacaagtg ggaatacccc tggtatgccg
     4321 cctgggacct ggggtttcat tgccttgtat ttgcgatgat cgacgctgaa tttgcgaagc
     4381 atcagttgtt actgctgaca catgactggt atatgcaccc gaacggtcag ctgccggcat
     4441 acgagtggtc cttcggcgat accaatccgc cggtacatgc ctgggcggct tatagagtgt
     4501 atgagatcga taaagcgaat aacggcggga ccggagacat tgccttcctg gagacggtat
     4561 tccacaagct cctgctcaac ttcacctggt gggtgaaccg aaaagacgag gaaggaaaca
     4621 atatcttcga gggcggcttc ctgggcatgg acaatatcgg gctttttgac cgaagcaata
     4681 cccagctgcc cgatggcagt acgctggagc aagcggatgg tacgagctgg atggcgacat
     4741 attgcctcaa cctcatgcgg atcgcgctcg aactctcggt gactaat
//
LOCUS       DATGKE010000556         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_596737, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000556 DATGKE010000000
VERSION     DATGKE010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596737"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VKU83_09460"
     CDS             complement(<1..1288)
                     /locus_tag="VKU83_09460"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLI93826.1"
                     /translation="MRLPRRAFLKTAGCLTIGFCLTESFIAKARAAASPGSLGQPGPL
                     PGDLRENPRIDSWLQVLEDGRVRVLTGKIELGQGIRTAVAQMAAEELDLDMTQVEVLM
                     AETGVTPDEGYTAGSNSIEASAIAVRYAAAATRQKLLDMAAQKLGTPAADLTFSKGAI
                     TARNGNSISLAALLNGKQISDEVHMPVAIKPKEQHHLVGKPVPRPDNEPIVFGRPYYV
                     HDLQLPGMVYGRIVRPPVYGVQLRQIDENAVRQQFPDLLKIVVNGSFLGVIATSEYGA
                     IKAQNLVRKHSKWTEGPLLPKLTPEQLPDYLRSLPVRSENVHRQGDLSAATTIKASYF
                     KPYIMHGAIGPSCAVAHYENNHLDVWSHSQGAYPLRESRAALIDLPSKNIHVKGLPGS
                     GCYGHNGADDVAADAALLAMAYPGKPVHVQWAREDEH"
     gene            complement(1354..>2032)
                     /locus_tag="VKU83_09465"
     CDS             complement(1354..>2032)
                     /locus_tag="VKU83_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HLI93827.1"
                     /translation="GSVNATFEGYPNGYYYGRRPTEVFLPNFRNLRHDEGLGFKRGYM
                     SYFSAARVGWQKGDYQNGIGGAWKDSLAEPGIWWVSMGMQGETMPKFDNHVRLSPDRK
                     DEWGVPQLITAIAYDDNDDKMAADFVARGSEMLEAAGCSTISGGNNHQNPGLDIHEMG
                     GCRMGKDPRTSLLNEWNQLHTCPNVFVTDGACMTSTGNQNPSLTFMALTARAANHAVE
                     EMKKQNL"
BASE COUNT          399 a          506 c          670 g          457 t
ORIGIN      
        1 catgttcatc ttcgcgtgcc cactggacgt gcaccggctt accgggatag gccatggcga
       61 ggagggcggc atcggcggct acatcgtcgg ccccgttatg gccataacag ccggagccgg
      121 gaaggccttt aacgtggatg ttttttgaag gcagatcgat gagcgccgcc cgggattctc
      181 taaggggata tgcgccctgg ctatgggacc agacgtcgag gtggttattt tcgtaatgcg
      241 caacggcgca agagggcccg atagcgccgt gcatgatgta gggtttgaaa tagctggctt
      301 tgatggtggt agctgcggat agatcgcctt gccggtggac gttctcggag cgaacaggca
      361 gggaccgaag gtaatcgggg agttgttcgg gggtaagctt aggcaagagt ggaccttcag
      421 tccatttgga atgttttcgt acgaggtttt gggctttgat ggcgccgtat tcgctggtgg
      481 cgatcacgcc gaggaagctg ccgttcacga cgatcttcag gagatcggga aattgttgcc
      541 tgaccgcgtt ttcgtcgatc tgtcggagct gcacaccata gacgggtggg cgaacgatgc
      601 ggccatagac cataccggga agctgtaaat catgaacgta ataaggccgg ccgaagacga
      661 tgggttcatt gtccgggcgg ggaacgggct tacctaccag gtgatgttgc tcttttggtt
      721 taatagcaac gggcatatgg acctcgtcgg agatctgttt gccgttgagg agagcggcga
      781 ggctaatgct attgccgttc ctggccgtga tggcgccttt tgaaaaggtg agatcggcgg
      841 caggcgtacc gagcttttgg gcagccatat cgaggagctt ttgacgggtt gccgcggcgg
      901 cgtagcggac ggcgatggcg ctggcttcga tggagttgct gccggcggta tagccttcat
      961 cgggcgtaac acctgtctcg gccataagga cttccacctg cgtcatatcg agatcgagct
     1021 cttcggcggc catctgggca acggcggtgc ggataccctg acccagctcg atcttgccgg
     1081 tgaggacacg gacgcgtcca tcttcaagaa cctgaagcca ggaatcgata cgcggattct
     1141 cccggagatc accgggaaga ggaccgggct ggccgaggct gccgggggat gcagctgccc
     1201 tggccttcgc gatgaagctt tcggtgaggc agaagccgat cgtcaggcaa ccggcggttt
     1261 tgaggaaggc tcttctcggc aggcgcatgg taggtatggc cggttttagc gactcatcga
     1321 taagtcggga tgccgtgcat aaagttacga aggttacagg ttttgttttt tcatttcttc
     1381 cacggcatga tttgcggcgc gggcggtcag ggccatgaag gtcagggaag ggttttggtt
     1441 gccggtagaa gtcatgcagg cgccgtcggt cacgaagaca ttggggcaag tgtgcagttg
     1501 gttccattcg ttgagcaggg aggtcctggg gtccttgccc atacgacagc cgcccatctc
     1561 atggatatcg aggccagggt tctggtggtt gtttccgccg gagatagtac tgcagccggc
     1621 ggcctccagc atctcggaac cccgggcgac gaagtcggca gccattttat catcgttgtc
     1681 gtcataggcg atagccgtaa tgagctgagg gacgccccat tcgtctttcc gatcggggct
     1741 aaggcggacg tggttatcga atttgggcat ggtctcgccc tgcatgccca tggagaccca
     1801 ccagatcccg ggctcggcaa gcgaatcttt ccaggcaccg ccgataccat tttggtagtc
     1861 acctttctgc catcctactc tggcagctga gaaataggac atatagccgc gtttgaaacc
     1921 cagcccttcg tcgtgacgga ggttgcgaaa attagggagg aagacctctg ttggccggcg
     1981 gccatagtaa taaccattcg gataaccctc gaaagtagcg ttcacggagc cg
//
LOCUS       DATGKE010000557         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1066095, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000557 DATGKE010000000
VERSION     DATGKE010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066095"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="metK"
                     /locus_tag="VKU83_09470"
     CDS             complement(<1..927)
                     /gene="metK"
                     /locus_tag="VKU83_09470"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLI93828.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAFDPQSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIARGVIRRIGYTKSEYMFEADSCGIFSAIHEQSSDI
                     NQGVDRKKKEEQGAGDQGMMFGYASNETDDYMPLSLDLAHKILLELAVLRRENKQIKY
                     LRPDAKSQVTLEYDDNNKPVRIDAIVVSTQHDEFDTEAKMQAKIKKDIIEILIPRVKA
                     KYKKYARLFNDQIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGVADEVLVQV"
     gene            complement(1012..1824)
                     /locus_tag="VKU83_09475"
     CDS             complement(1012..1824)
                     /locus_tag="VKU83_09475"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HLI93829.1"
                     /translation="MLQKKRILFIANEMSPYLELTEFAEIVNKLAIKSNDNGFEVRCI
                     MPRFGVVNERRHRLHEVVRLSGINVSVDNDDYPLQIKVASLPNARLQVYFLDNEDLFK
                     RKYIFHDEQDKWFEDNGLRTVFYCKGALETVKKFGWPPDVIHCSGWMTGLIPLYLKTV
                     YKKEPVFAHSKCVFTIGQSTFKEKLGADFLKLANIHANIKEKDLEPYKDANNSALFRG
                     GATYADAISFGAEKVDKKLVEEFGKTRGKKVLAFNAESDLTDYLQLYTDLSK"
     gene            1944..>2459
                     /locus_tag="VKU83_09480"
     CDS             1944..>2459
                     /locus_tag="VKU83_09480"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLI93830.1"
                     /translation="MILFKRAADLTGWLEREKAAGRTVGFVPTMGALHAGHMSLITIS
                     KKATAITVCSIFVNPTQFNDALDYEKYPVTLEKDIAMLEQAGTDVLFLPGLAELYPAG
                     IKALEHYDLGELDKVLEGRFRPGHFQGVCQVMSRLLQLVRPDRLFLGQKDYQQCLVIQ
                     RLLSLMGLPAVL"
BASE COUNT          527 a          657 c          638 g          637 t
ORIGIN      
        1 tacctggaca agcacctcgt cggctacgcc ggcagctacc aggttcttgg cgatgtgccg
       61 ggtagcatag gcagcggagc ggtcaacctt ggagggatcc ttcccgctga aggcgccgcc
      121 tccatgtgca ccctttccgc cgtaggtgtc cacgatgatc ttgcgccccg tcaggccggt
      181 gtcaccgtgg ggaccaccga tcacaaactt tcccgtcggg ttgatatgat atttgatctg
      241 atcgttgaac agccgcgcat actttttgta tttggccttt acacgcggta tcaggatctc
      301 gatgatgtcc tttttgatct tcgcctgcat cttcgcttcc gtgtcgaatt cgtcatgctg
      361 cgtcgatacg acgatcgcat cgatcctcac gggcttgtta ttgtcgtcgt actccagcgt
      421 cacctggctt ttcgcatccg gccgcaggta cttgatctgc ttgttttccc ggcggagcac
      481 agccagttcg agcaggatct tatgggccag gtccagtgac aagggcatat agtcgtcggt
      541 ctcattgctg gcataaccga acatcatccc ctggtcgccg gcgccctgtt cttctttctt
      601 cttcctgtcg acgccctgat tgatatcgga agattgctcg tgaatggcag aaaagatgcc
      661 gcaggaatcg gcctcgaaca tgtattcgct cttcgtgtac ccgatccggc ggatgacccc
      721 gcgggcgatt tcctgcacat ccaggtaggc cttggacttt acttctccgg caaggaccac
      781 ctgtcccgtc gtcacaaggg tttcgcatgc caccttggac tgggggtcaa aggcaaggaa
      841 attgtcgatc aacgcatcgg atatctgatc ggccacctta tcgggatgcc cttcagaaac
      901 gctctcggaa gtaaataaat aaggcatttt tgaaaagaat gtttagttga aaacgaatgg
      961 gtaatttttt acgcgccgca aatatacggc ttcgatccgc tcataaacgc attatttgct
     1021 caggtcagta tacaactgta aataatctgt caggtccgat tcggcattga atgccaggac
     1081 cttttttccg cgggttttgc cgaactcctc gacaagcttc ttgtcgacct tttcggcccc
     1141 aaaactgatc gcatcggcgt aggtggcgcc gccacggaag agggcgctgt tatttgcgtc
     1201 cttatagggt tccagatcct tttccttaat atttgcatgg atattggcca gcttcaggaa
     1261 gtcggccccc agcttttctt tgaaagtgga ctgaccgatc gtgaaaacgc atttgctgtg
     1321 agcaaaaacg ggttctttct tataaaccgt cttcaggtac agcggaataa gccctgtcat
     1381 ccagccgctg cagtggatca cgtccggcgg ccagccgaat ttctttacgg tctcgagtgc
     1441 tcccttgcag taaaatacgg tgcgcagccc attgtcttcg aaccatttgt cctgttcgtc
     1501 atgaaagata tatttgcgct tgaaaagatc ctcgttgtcc aggaaataga cctgcagccg
     1561 ggcattggga agggaagcta ctttgatctg caggggatag tcgtcattgt ccaccgaaac
     1621 gttaatgcct gaaagcctga ccacctcgtg aagccgatgc cgccgctcgt tcactacccc
     1681 aaaccgcggc atgatgcaac gcacctcgaa cccattgtca tttgatttaa ttgccagctt
     1741 gttaacgatc tctgcaaatt ctgtcagctc taaatatggc gacatttcgt tggctataaa
     1801 taaaattcgc tttttttgta gcattattat tacgataagt gttgagacga accctgcgaa
     1861 atagtttgca aaattacgat ttttattgga aaatcggtgc tggggccgct tccggggcct
     1921 aaaaccggtt aaaaccgtgc aaaatgatcc tatttaaacg ggccgcggac ctgaccggct
     1981 ggctggaacg ggagaaggcc gctggccgga ccgtcggatt cgtaccgacg atgggtgcgc
     2041 tccacgcggg acatatgtcc cttatcacta tatcaaaaaa ggcgaccgcg atcaccgttt
     2101 gcagcatatt cgtgaatccc acgcagttca atgatgccct tgattatgaa aaatatccgg
     2161 ttacgctgga aaaggacatc gccatgctgg agcaggcggg aacggacgtc ctgttcctgc
     2221 ccggtctcgc cgaactgtat ccggccggga taaaagccct tgaacattat gatctgggcg
     2281 aactggataa ggttctggag ggccggttcc gccccggcca ttttcagggc gtgtgccagg
     2341 tgatgtcccg gctcctgcag ctcgtgcggc cggaccggct ctttttgggc caaaaggact
     2401 atcaacagtg cctggtgatc cagcggttgc tttcgctgat ggggctgccg gcggtgctc
//
LOCUS       DATGKE010000558         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_509097, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000558 DATGKE010000000
VERSION     DATGKE010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509097"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VKU83_09485"
     CDS             <1..1004
                     /locus_tag="VKU83_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI93831.1"
                     /translation="KAAGILSGITSTGDYNTVLSQIIPKACQTGHGGVLTPTMWIHNI
                     NTLLARNIICNKLAPYVNGNPSATIAKSSTSAMSTSDWNNVLTLAGNGIQPGDYIFSA
                     HSTSASSIFTPTSGNAAAETTGNNISSTFKISVRYIQCFKPADKRLLTDFRLGSTYNN
                     AFYGTPYNIVDSLQQSANGVYVIGSKQVGQYEAFITSYEENALMLAEANIRTGNIDKG
                     LSYVDAVRTYQGAGVPAVSGTGLTLAQAMQELTNESRVALVFRGLSFYNSRRWGWIYD
                     ISNGGGQYGQKLYNTNGTYFTNVTIDYNFMDYWDIPADETTLNAPGTGSAPILNPNY"
     gene            1010..1717
                     /locus_tag="VKU83_09490"
     CDS             1010..1717
                     /locus_tag="VKU83_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93832.1"
                     /translation="MKRILLLALLTSGLGRVCAQDGFVSQSGAPLSYNVFDHNGKAFV
                     NPAPEVEGTPFLTDKWRMGTLVVTTNRRYDSVKIRINLLSQEVHVLDRNNTEIALAKG
                     YIKQVIWTDAVKGLPTGGTCFQSGFPAVDNQDKDFFYEVLSQGKLWLLHSVRKVITER
                     KDGLSGDIRKDYTTYDDYYVYDGKNMQRVRKDKSFILTIAGDKRDKIDAFLEANKLKA
                     RSAEDLRRIIDYYNTLP"
     gene            1918..>2289
                     /locus_tag="VKU83_09495"
     CDS             1918..>2289
                     /locus_tag="VKU83_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI93833.1"
                     /translation="MTVFMVISIGLLAQTSEVSGRVTDPTGSPIPNATIRVKGVRGGT
                     SADANGNFKIRAAGNATLIISAIGFEQTEIPIDHRTSVTIGLKSSNLALSEVVVTALG
                     VKREKKALGYAVTTVGKQDVEL"
BASE COUNT          584 a          630 c          588 g          487 t
ORIGIN      
        1 acaaggcagc cggtatcctc agtggtatca ccagcacggg agattataat acggtgctga
       61 gccagataat tccaaaggcc tgtcaaaccg gtcacggcgg ggtgctcacg cccaccatgt
      121 ggatacacaa catcaatacc ctgctggcca ggaatatcat ctgtaacaaa ttagccccct
      181 atgtgaacgg caaccccagc gccaccatcg ccaaatcgtc caccagcgcc atgagcactt
      241 ccgattggaa caatgttctg acgcttgcgg ggaatggcat ccagccggga gactatatat
      301 tctccgcgca ctcgacgagt gcctccagca ttttcacccc tacgagcggc aatgcggcgg
      361 ccgaaaccac cggcaacaat atctcgtcca cctttaagat cagcgtccgg tatatccagt
      421 gtttcaagcc ggcagacaaa cggctgctca cagatttccg actgggcagt acctacaaca
      481 atgcctttta cggtacaccc tataacatcg tagacagcct tcagcaaagc gctaacggtg
      541 tttatgttat cggcagcaaa caggttggcc aatatgaggc ctttattaca agctatgaag
      601 agaatgccct gatgctggcg gaagcgaaca tacggaccgg caacatcgat aaagggcttt
      661 cctatgtcga tgccgtacgc acctaccagg gggcgggcgt gcctgccgtg tccggtaccg
      721 gactgacact tgcacaggcc atgcaggagc tgaccaatga gagccgggta gcgcttgtat
      781 tccgcggcct ttctttctac aactcccggc gctggggctg gatctacgat atcagcaatg
      841 gcggcggtca gtatggccag aagctgtata atacgaacgg cacttatttt accaacgtca
      901 ccattgatta taattttatg gattactggg atataccggc agacgaaacg accttgaatg
      961 cgcccggcac cggcagcgct ccgatcctga acccgaacta ttgaacgaca tgaaacgcat
     1021 tctcctactg gcattgttga cgagcggtct cggccgggtc tgcgctcagg acggttttgt
     1081 atcccagagc ggagccccgc tctcctataa cgtcttcgac cacaacggca aggccttcgt
     1141 caatccggcc ccggaagtgg aaggcacacc ctttcttaca gacaagtgga ggatggggac
     1201 gctcgtggtc accaccaacc gccggtatga cagcgtaaag atccgcatca atctgttgtc
     1261 ccaggaagtg catgtgctcg acaggaacaa caccgagatc gcgctggcga aagggtatat
     1321 caaacaggtg atctggacgg atgccgtcaa gggcctgccc accggcggga cctgtttcca
     1381 gagcggcttc cctgcggtgg ataatcagga caaggatttt ttctacgagg tcctttccca
     1441 gggtaagcta tggctcctgc actccgtccg gaaggtcatc accgagcgga aagacggtct
     1501 gtcgggggat atcaggaaag attataccac atatgacgat tattatgtgt atgacggcaa
     1561 gaacatgcag cgggtaagaa aggataaatc gttcatcctg accattgcgg gcgacaagcg
     1621 ggacaagatc gacgcgttcc tggaggcgaa taagctaaaa gcccgatccg ctgaggacct
     1681 cagacggatc atcgattatt acaataccct tccttaatag ggtcaagatt gtgtgactca
     1741 atgttgacag tgaaagacgg ccccggtgtc taccggggcc aattttttgg gtacttcaaa
     1801 agaatttctt atattcatcg cacttcactg ttcagcagca gggtcatccc gaccattgct
     1861 aatttagcat ttatccataa ccaactacta cacatgagac aactacactg ttggctgttg
     1921 accgtattca tggtcatcag cattgggtta ctcgcccaga cgtcagaagt ctccgggagg
     1981 gtcacagatc caaccggctc accaattccc aacgcaacca ttcgcgtcaa aggtgttcga
     2041 ggaggcacca gcgccgatgc caacggcaat ttcaagataa gggccgcagg caacgcgacg
     2101 cttattatct cggcgatcgg cttcgaacag accgagatcc cgatcgatca tcggacatcg
     2161 gttaccatcg gacttaaatc ctccaatcta gctcttagcg aagtcgtcgt tacggcgctg
     2221 ggcgtcaaac gcgaaaaaaa ggcacttggg tatgccgtga ccacggtagg caagcaggat
     2281 gtcgagcta
//
LOCUS       DATGKE010000559         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1028069, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000559 DATGKE010000000
VERSION     DATGKE010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028069"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1099)
                     /locus_tag="VKU83_09500"
     CDS             complement(26..1099)
                     /locus_tag="VKU83_09500"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLI93834.1"
                     /translation="MEQQNTQKKGGKKKLVFPIILAVVLVGGLIFGIKEYIYYSKHET
                     TDDAQVDADISPVVARVTGYVKEIRFADNQYVHAGDTLVILDDRDYQLKLQQALAALT
                     STKQTVDVSQQQVSEARTSIATAQANVDAAKVAVWKANEDFTRYQNLYNDHAITKAQY
                     DDAKAAKESAEAALAIAQTQVPTQHRRVDVSQRQVGATSSNLATRQADVDYARLQLSY
                     TVITAPASGIVSKRNIQLGQLVQAGQTLFAIVNTNNMYVTANFKETQMEPLRIGQRVD
                     ISVDAYPDSTFHGQIESVSGATGAKFSLLPPDNATGNFVKVVQRVPVKIVLEPGENRE
                     HLLLPGMSVTPTVFLNSGRNNAY"
     gene            complement(1136..2455)
                     /locus_tag="VKU83_09505"
     CDS             complement(1136..2455)
                     /locus_tag="VKU83_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93835.1"
                     /translation="MEKRLTGVIGFLLLAGLLANAQPRKLSLEEAVQLGIQNSKELKR
                     QQFKIDEALARLDQARDAQLPSMKVSFQYLHALMLSRVVSIPGLTKEPIKLPFDFPAY
                     MGTLSVTEPIFAGNQLKYAKQSADLMVQLSRLDGDQDKEDVTSVVINAFLNFDKILEN
                     QLIVAQNMQDIQGKLDEITKYESQGLATQNDVLRYQLQKSQMQLTEIELENNRKVANY
                     DMDVMLGLPDTTELLPDTVNYKLDENLALPDLLQQAETNRRELLDLSYQTRLADLNIK
                     KIHDQRLPTVAASAGMYYINPTGEVIPTRNNLLAPITLGIGVSWDIGTLYTNRNKERE
                     ADLQRKELNTARDEGTDEIRMDVEKQFIAYQQALARIKVLQVAVQQAEENERITESKF
                     RNNLVNTTDRIDAQTMLYQSRVNLELAKSDATIAYYNVLKSTGKIHI"
     gene            complement(2469..3119)
                     /locus_tag="VKU83_09510"
     CDS             complement(2469..3119)
                     /locus_tag="VKU83_09510"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLI93836.1"
                     /translation="MGDKKEHILTVAEELFAEKGFDGASVRDIAQRAGVNLAMISYYF
                     GSKEKLLESLIEFRAGYAYGILDELNKDSSLSPWDKIDRLVDFYVDRIINNLRFHTIM
                     WQESGARSEEIKNRTIAIKLRNLEQIVKIITDGQQKKLFRQVDIPMTVGTIMGTISYY
                     TQSKTYACVSLAMQESLNDEQYRQKLSARLKVHLKRLLRAHLDVRNDAQNDEQPKI"
     gene            complement(3243..3839)
                     /gene="lptC"
                     /locus_tag="VKU83_09515"
     CDS             complement(3243..3839)
                     /gene="lptC"
                     /locus_tag="VKU83_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HLI93837.1"
                     /translation="MHSWRLAIVLAGMVLYGCGEKTNQQDGNGPKTEVPEEGTNIQAY
                     LSETGIDMGKLEAKVKGKLRAPFMYHYQRADSPYFEFPRTLHVDFYEDSVMVNERPTI
                     ESQLDALYGKYLPNQDKVYLRDSVVVKNILKGDTLHCKYLWWDQHTQRFSTDDSVRIN
                     TRDKILFGTGMEADQNFRWYTIRHMTGIVLTSGNNIPK"
     gene            complement(3832..4557)
                     /locus_tag="VKU83_09520"
     CDS             complement(3832..4557)
                     /locus_tag="VKU83_09520"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLI93838.1"
                     /translation="MPTTLCFDFGNTRMKCAVFTDGTLQQQAVLGADPEQAVSLLLDQ
                     FQPTRTILSSVIDHPPGIEALLRSRSRFHRLDHSSKLPITTPVSKPDSIGADRLALTA
                     AAVSLFPGKNNLVIGLGSAVTYNFVNKYCEFLGGGISPGMEMRFRSLHEFTAKLPLVK
                     PDWNLPLVGYDTRTNILSGVILGMAKEIDGTIGAYEEKYDNFNVLMSGGDAAYFTGRL
                     KKEIFVDPDLIFKGLYAISELNA"
     gene            complement(4559..5203)
                     /locus_tag="VKU83_09525"
     CDS             complement(4559..5203)
                     /locus_tag="VKU83_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLI93839.1"
                     /translation="MTNFIPVFPLGIVVYPGEMVNLHIFEPRYRQLVNECYAESKPFG
                     IPTVIDNRLNEMGTLVRITERVKLYESGDMDIRTEGLRVFRILELIKSVPDKLFSGAI
                     VNYPDNVEGPGKPALMAKIIAATRELHRLLRISKDFHKPDAQLVSYDLAHHAGMSLQQ
                     EYELLGLLREEQRQEYLKRHLGKVLPVMAEMEVLKEKVKLNGHFKNLSSFKFED"
BASE COUNT         1079 a         1483 c         1404 g         1375 t
ORIGIN      
        1 actgggtgcg tgtctggcag aaagttcagt aggcgttgtt gcgcccgctg ttcagaaaca
       61 ccgtgggcgt taccgacatg ccgggcaaca gcagatgctc gcggttctcg cctggctcga
      121 gcacaatttt caccggaacg cgctgcacga ctttcacaaa attacctgtt gcgttatccg
      181 gcggcagcag cgagaatttc gccccggtgg cgccggaaac agattcgatc tggccatgga
      241 aggtgctgtc gggataggcg tcgaccgaaa tatcgaccct ctggccaatc ctcagcggct
      301 ccatctgtgt ttccttgaaa ttggccgtaa catacatatt gttcgtgttc acgatcgcga
      361 acaaggtctg accggcctgc accaattggc cgagctggat attgcgcttc gaaacgatcc
      421 cgctggcagg agcggtgatg accgtatagg ataactgcag cctggcataa tcgacgtccg
      481 cctgccgggt ggcgaggttg gaagaagtgg cgcctacctg ccgctgggaa acatccaccc
      541 gtctgtgctg ggtcggcacc tgcgtttggg cgatggccag cgcggcttcc gccgattctt
      601 tggcggcttt cgcgtcatcg tactgcgcct tggtgatcgc gtgatcgttg tataaattct
      661 ggtaccgcgt gaaatcctcg ttggccttcc agacggcgac cttcgccgca tcgacattgg
      721 cctgcgcggt ggcgatgctg gtccgcgctt cggagacctg ctgctgggaa acatccacgg
      781 tctgtttcgt cgaagtaagg gcagcaagcg cctgctgcag cttcagctgg tagtcccgat
      841 cgtcgagaat gacgagcgta tcaccggcgt ggacgtactg gttatcagcg aagcggatct
      901 cttttacata cccggtaacc cgggcgacca ccgggctgat gtcggcatcg acctgggcat
      961 catccgtggt ctcgtgcttg ctatagtaga tatattcttt gatcccaaag atgaggccgc
     1021 ccaccagcac gactgccagg atgatcggga ataccagctt ttttttgccg cctttcttct
     1081 gtgtgttttg ttgttccata taaattcaac tatttgaacg cctttagaat tggttttaga
     1141 tgtgaatctt tcccgtcgat ttcaaaacat tataataagc gatggtcgca tcggacttcg
     1201 ccagctcgag gtttacccgc gactggtata acatcgtctg cgcatcgatc cggtcggtcg
     1261 tgttgacgag gttgttccgg aatttggatt ccgtgatccg ctcgttctcc tcggcctgct
     1321 gcaccgccac ctgcaggacc ttgatcctgg ccagcgcctg ctggtaggca atgaactgct
     1381 tctcgacatc catccggatc tcatcggtac cctcgtcccg ggcggtattc agctccttgc
     1441 gctgcaggtc tgcctcgcgc tccttgttcc tgttcgtata cagcgtcccg atgtcccagg
     1501 agacgccgat gccgagggtt atgggagcca acaggttgtt tcgggtcgga ataacctcgc
     1561 ccgtcggatt gatataatac atgcctgccg aagcagcgac ggtcggcagc cgctggtcat
     1621 ggatcttttt gatgttgagg tccgcgagcc tggtttgata cgaaaggtcg agcagctcgc
     1681 gccggttcgt ctcagcctgc tggaggaggt cgggaagcgc caggttctcg tccagtttgt
     1741 agttgaccgt gtcgggcaac agttcggtgg tgtccggcag gcccagcatc acgtccatat
     1801 cgtagttggc gaccttgcga ttattctcca gctcgatttc ggtcagctgc atctgcgatt
     1861 tctgcaactg gtatcggagg acatcgtttt gagtagccag tccctgactt tcgtatttgg
     1921 tgatctcgtc gagcttccct tgaatgtcct gcatgttttg cgcaacgatc aattgatttt
     1981 ccaatatctt gtcaaaattc aaaaaggcgt tgatgactac ggaggtaacg tcttccttgt
     2041 cctggtcgcc gtcgagccgg ctcagctgca ccatcaggtc cgcggattgc tttgcatatt
     2101 tcagctggtt tccggcgaag atcggttcgg tcaccgacag tgtgcccata taggccggaa
     2161 aatcaaaagg aagcttgatg ggttccttgg ttaaacccgg gatgctgacc acgcggctca
     2221 gcatcagcgc gtgcaaatat tggaaactta ccttcatgga aggcagttga gcgtcgcgcg
     2281 cctggtcaag gcgcgcgagc gcttcgtcaa tcttaaattg ctgtcgtttc agttccttgc
     2341 tgttctgtat gccgagctgt accgcctctt ccaggctcaa tttcctaggt tgggcgttgg
     2401 ccagcaaacc tgccaggagc aggaagccga taacccccgt tagccttttt tccatgttgt
     2461 tcatgtattt atatcttcgg ttgttcatca ttttgcgcat cgttccggac atcgagatgc
     2521 gccctcagca aacgcttgag gtgaaccttc agtctcgcgg aaagcttctg gcggtattgt
     2581 tcgtcattca ggctttcctg catggcaagc gaaacacagg cgtaggtctt cgattgcgta
     2641 taataagata tcgttcccat gatcgtcccc accgtcatcg ggatatccac ctggcggaat
     2701 agctttttct gctggccgtc ggtgatgatc ttcacgatct gttcgagatt gcgaagtttg
     2761 atggcgatcg tcctgttctt gatctcctcg gaccgggcgc cgctttcctg ccacataatg
     2821 gtatggaacc ggaggttgtt gatgatcctg tcgacataaa aatccaccag cctgtcgatc
     2881 ttatcccagg ggctgagcga tgaatccttg ttcagctcgt ccaggatccc ataggcgtat
     2941 cccgcgcgga actcgatcag cgattccagg agcttttcct ttgaaccgaa ataatacgag
     3001 atcatcgcca ggttaacacc agcccgctga gcgatatccc ggacagacgc accgtcaaac
     3061 cctttttccg cgaaaagctc ctctgccacg gtaaggatat gctctttttt atcgcccatg
     3121 ttgaagtttt ttgttcgttg atctcatcaa tggcgcaaag ttaaacaaac gtttgaatta
     3181 aacaaacgtt taattgactt ttttggagat ttttctcccc ccggcttagg cctgataata
     3241 aatcatttag ggatgttgtt cccggaagtg agaacgattc cggtcatatg ccggatcgtg
     3301 taccagcgaa aattctggtc ggcctccata ccagtgccga acaggatctt gtcccgggta
     3361 ttgatccgca ccgaatcgtc ggtcgagaaa cgctgggtgt gctggtccca ccagaggtat
     3421 ttgcaatgga gcgtatctcc tttcaggata ttctttacca caacggaatc gcgcaggtag
     3481 accttgtcct ggttcggcag gtacttgcca tatagcgcgt cgagctggct ctcgatcgtc
     3541 ggtctttcat ttaccatcac ggaatcctcg taaaaatcca catgcagggt ccgcggaaat
     3601 tcgaaataag gggagtccgc gcgttgataa tggtacatga atggcgcccg cagcttgcct
     3661 ttcaccttcg cctccagttt gcccatatcg atcccggtct cactgagata agcctggata
     3721 ttggtcccct cctcgggcac ctctgtcttc gggccgttac cgtcctgctg gttggtcttt
     3781 tcgccgcagc cgtaaagcac catccccgcc agcacgatcg ctaacctcca actatgcatt
     3841 gagctcactg atggcatata agcctttaaa aattaaatcc gggtcgacaa atatctcctt
     3901 tttcaaccgc cccgtgaaat aggctgcgtc gccgccgctc atgagcacgt taaaattgtc
     3961 atatttctct tcatatgcac cgatcgtgcc atcgatctcc tttgccatcc cgaggatgac
     4021 accgctcagg atattcgtcc tggtgtcata tcccaccagc gggaggttcc agtcaggctt
     4081 tacaaggggc aattttgcgg tgaactcgtg cagcgacctg aaccgcattt ccattcccgg
     4141 cgaaattccc ccaccgagga attcgcagta tttgttcacg aaattgtagg tgacagcgga
     4201 acccaaacca atcaccaggt tatttttccc tgggaacagg gataccgctg cggcagtcag
     4261 ggcaagcctg tcggcgccaa tgctgtccgg cttgcttacc ggcgtcgtaa tcggcagttt
     4321 cgatgaatga tccagccggt gaaaccggct ccggctgcgc agcaaggctt cgatgccggg
     4381 cgggtggtcg atcaccgaag agagaatggt gcgtgttggc tggaactggt ccagcaataa
     4441 gctgaccgcc tgctccggat cggccccgag gaccgcctgc tgctgtaacg taccatcggt
     4501 gaaaaccgca catttcattc gggtgttgcc aaaatcgaaa cagagcgtcg tgggcatgtc
     4561 agtcctcaaa tttgaacgaa gataagtttt tgaaatggcc gttcagtttt accttctcct
     4621 tcaagacttc catttcggcc atgaccggca aaacttttcc cagatggcgc ttcaggtatt
     4681 cctgccgctg ctcttcccgt aacaacccca acagctcata ttcctgctgc agcgacatcc
     4741 cggcgtggtg cgcgaggtca tagctcacca attgggcgtc gggcttgtgg aaatccttgc
     4801 tgatcctgag cagccggtgc agctcgcggg tcgctgcgat gatcttggcc atcagcgcag
     4861 gttttccggg gccttccacg ttgtccggat agttcacgat cgctccgctg aatagcttgt
     4921 ccggaacaga ttttatcagt tccaggatcc ggaagacgcg caatccttcc gtccggatat
     4981 ccatatcgcc gctctcgtac agcttgaccc gttccgtgat gcgcacgagc gtacccatct
     5041 cgttcagccg gttatcaatt acggttggga tcccgaacgg cttactctcc gcgtaacatt
     5101 cgttgaccag ttgcctgtac cgcggctcga agatgtgcag gttcaccatc tcaccgggat
     5161 acaccacgat acccaatgga aacacgggga taaaattggt catagtctgc aaaaataagt
     5221 cattttgcag gtaccttctg cagggtctgg agccagcgcc ccacctcttg cggttccaca
     5281 tcccggatac acacgcagtc tccgcttcgg cggcatgcgt cgcagtaagt ttccttgacg
     5341 a
//
LOCUS       DATGKE010000560         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_39391, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000560 DATGKE010000000
VERSION     DATGKE010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39391"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1015)
                     /locus_tag="VKU83_09530"
     CDS             complement(5..1015)
                     /locus_tag="VKU83_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLI93840.1"
                     /translation="MEVVHLFRKKGAFFSIERVFARLEPELNKFVTVRRVVAPSGGSS
                     LPAIVRNLRVARRVRADIYHVTGDLHYLTLGLPRKRTILTIHDCVFLYQTKGVKRLLL
                     KWLFLSWPVRHCALVTTISESTRQDIIRHTGCAPGKVVVIPNPVDSKISFSSRPFHGE
                     EPVFLFLGSTPNKNLLRTIPALEGIPCLLDVVGDISTEAIAQLQRHKIRYRQASNLTD
                     EEVAEKYCGADVVLFPSTFEGFGLPVIEAQQAGRPVVTSDLDPMREVAGAAACLVDPY
                     AVEAIREGILRVVHDREYREELVRKGFENVKRFATAAIVHQYVSCYQGLLPLDRVSTP
                     DK"
     gene            complement(1016..>2311)
                     /locus_tag="VKU83_09535"
     CDS             complement(1016..>2311)
                     /locus_tag="VKU83_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93841.1"
                     /translation="IVLRELIALYGLFVCVLMPMVGYTTYTKANFLARLWVRWMFIPK
                     ETYFSYALPAMTLFCLGICWPMNSAKASDQGVLLRSTIDRIQKKLSGMPKTSIYIISI
                     GIAANFIGSFLPEALTFVATLFFWGSFAGVLYLFYTPAFKYKKLILVIFAFFILSTAL
                     RNGMFTLVAYMGITMFSFFFLGKKTRFWKKLTFFLLGCLFLLLTQSIKSSYRNYLWRQ
                     GYAGNTTVLFGSLLIDKISDFSSLFSADAFFPIYYRTNQGFNVAMVMSRFPSSVPYDY
                     GANLSLAFASSFVPRLFWPDKPEAGGKFNMKYYTGRIIQGWSTNVGPLGEAYGSFGPV
                     GGMIYMFFLALFIRWAYKLIFKLSAKTPLLIFWIPVMFYQITYSSETDSLQIFNSLIK
                     AGFFVWLLAKFLPDWFGIQKRLRRRSQVRNLESASTPIS"
BASE COUNT          625 a          596 c          614 g          476 t
ORIGIN      
        1 atttttattt atcaggtgtt gagaccctat ccaacggtaa cagcccctga tagcaggaaa
       61 catattgatg gacaatcgcc gctgtagcga accgcttcac attttcaaat cccttccgta
      121 ccaattcttc ccggtattcc ctgtcatgga cgaccctgag gatgccttcc cggatagctt
      181 ccactgcata gggatccacg aggcaggcgg cggcgccggc cacttccctc atggggtcca
      241 gatcactggt gacaacgggc cggcccgcct gctgggcttc gatcaccggc aatccgaagc
      301 cttcgaaagt ggaagggaac agcacgacgt cggcgccgca atatttttct gcgacttcct
      361 cgtccgtcag gtttgaagcc tgccggtacc tgatcttatg acgttgcaac tgggcaattg
      421 cctctgtgga aatatcgcct acgacgtcca gcaaacaagg aattccctca agcgcaggga
      481 tagtcctcag gaggtttttg ttcggagtgc ttcccagaaa gaggaatacc ggctcttcgc
      541 cgtgaaaagg ccgtgatgaa aaggaaatct tgctgtccac cgggttgggg atgacgacaa
      601 ccttgcccgg ggcgcaaccg gtatgacgga tgatatcctg ccgcgtagat tcggaaatcg
      661 tcgtgacgag cgcgcaatga cggacaggcc agctcaggaa cagccacttg agcaggaggc
      721 gtttgacacc cttcgtctgg taaaggaaga cgcaatcatg gatggtgagg atcgtccgct
      781 tccggggcag gccgagcgtc aaatagtgca agtctccggt gacgtggtaa atatccgccc
      841 tgacgcgccg ggctacccgg agattccgga cgattgcggg gagcgaggaa ccgcccgatg
      901 gggcaacaac gcgccggaca gtgacaaact tattcagttc gggctccagt cgggcgaaga
      961 ccctttcgat actgaaaaac gcgccttttt tccggaacaa gtgaacaact tccatctacg
     1021 aaatcggggt tgaggcggat tcgaggttgc gaacctgcga ccggcgcctg agccgttttt
     1081 ggatcccaaa ccaatccggc aggaactttg cgagcagcca gacaaaaaat ccagccttta
     1141 tcaatgaatt gaagatctgc agcgaatccg tttccgatga atacgtgatc tggtagaaca
     1201 tcacgggtat ccaaaagatc agcaacggcg ttttggctga aagtttaaat atgagtttat
     1261 atgcccatcg gatgaaaagc gcaagaaaaa acatatagat cattccgccg acagggccga
     1321 aacttccata ggcttcacca aggggaccta cgttggtcga ccacccctgg attatccttc
     1381 ccgtataata tttcatattg aacttgccgc ctgcttccgg cttatccggc cagaacagcc
     1441 gcgggacaaa tgaggaggca aaagcgaggc tgaggttggc cccatagtcg tacggaacgg
     1501 aactgggaaa ccggctcatc accatggcaa cattgaaccc ctggttggtg cggtagtaga
     1561 tcggaaaaaa ggcgtctgcc gaaaaaagcg aggaaaaatc gctgatcttg tcaattaaca
     1621 gcgaaccaaa caaaacggtg gtgttgccgg catagccctg gcgccagaga taattcctat
     1681 agctgctttt aatgctttgc gtcagcagca ggaagaggca gcccaataaa aagaaagtca
     1741 gttttttcca gaaacgcgtc tttttcccga gaaaaaagaa cgaaaacatg gtgatgccca
     1801 tataggccac cagggtgaac attccgtttc gcaaggcggt gctaagaata aaaaaggcaa
     1861 atatgaccag gatcagcttt ttatacttaa aggccggcgt atagaaaagg tataaaaccc
     1921 cggcgaacga accccagaaa aacaaggttg cgacaaatgt caaggcttcc gggaggaagg
     1981 agccgataaa attagccgct atgccaatgg agatgatata gatgcttgtc ttcggcatcc
     2041 cggacaattt cttttggatc ctgtcgatag tggaacgcag caagacgccc tggtcgcttg
     2101 ccttcgcgga attcatgggc cagcagatgc cgaggcaaaa cagcgtcatc gcaggcaggg
     2161 cataactgaa atacgtctcc ttcggaatga acatccaccg gacccagagc ctcgccagaa
     2221 aattagcctt ggtgtaggta gtatagccga ccatcggcat caagacgcag acaaacagcc
     2281 cgtacaaggc aatcagttcc cgcaacacga t
//
LOCUS       DATGKE010000561         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1439711, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000561 DATGKE010000000
VERSION     DATGKE010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439711"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..857
                     /locus_tag="VKU83_09540"
     CDS             162..857
                     /locus_tag="VKU83_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLI93842.1"
                     /translation="MAEPTILITGATGGLGSVVVPYFLGKGYRVIAVVSEENAKNRPE
                     SHPLLDVQVVDLTDEGSVGSLIAWLIRRYGAIDGALLLAGGFVAGGVAEATGNEVKTM
                     FAINFETAYYVVRPVYKHMFARGKGRIVLVGARPALEPQAGKASVAYALSKSLLFRLA
                     EMLNADAGAKDVVTHVVVPSTIDTPNNRKSMPHADFDSWVKPEKLAETFYLICSGSAA
                     PSGDAVWKIYNNA"
     gene            850..1818
                     /locus_tag="VKU83_09545"
     CDS             850..1818
                     /locus_tag="VKU83_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HLI93843.1"
                     /translation="MRDEMENIKIATAQFEHASGDKEKNLRVIRRMTEQAATAGAQAI
                     AFHECSITGYSYARSLSKAGLLAAAEYIPEGPSVRALTEIARNSGVVVLAGLFEKDAE
                     NRIFKAYVAVGGSGLIAKFRKLHPFINPHITPGNGYVVFDLFGWKCGVLICYDNNVVE
                     NVRATALLGADILFMPHVTMCTPSTRPGAGFVDPRLWAARETDPTSLRIEFDGLKGRE
                     WLMKWLPARAYDNGIYVVFSNAIGMDDDQLKNGCSMILDPFGDILAECRNLGNQLVVA
                     TLTPEKLRLAGGYRYRMARRPDLYRDIIGAAHVPEQKVVWMDQGNE"
     gene            complement(1830..2273)
                     /locus_tag="VKU83_09550"
     CDS             complement(1830..2273)
                     /locus_tag="VKU83_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93844.1"
                     /translation="MQDPIKIVVCDDNGAESLLFGFTLRNIGIRHELEWVADSDELLY
                     YLAGVDRLPDIIFLDVNMPGKSGIECLEEIRSNPEWNHIPVIIQSFAGPTRDIEAAFN
                     GGADLFILKSDFSTEFQEALKQILQRDWSKRPRPRKSEFLLCGEN"
     gene            complement(2347..>2845)
                     /locus_tag="VKU83_09555"
     CDS             complement(2347..>2845)
                     /locus_tag="VKU83_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI93845.1"
                     /translation="AFLKALPHPPSVIITTAYHQYAVEGFNLSVTDYLLKPIEFDRFL
                     TAVNKVRSAHPPAAQPAAGPEARDFIFITVQKKRVKILFSEIVYIESQREYIRVVTTG
                     SEYLSKMSTNEIEGLLPPGLFKRVHRSYIVAIRRIESYTAEMIEIHGHSIPIGKAYRD
                     GMEKL"
BASE COUNT          649 a          731 c          858 g          607 t
ORIGIN      
        1 cgagcaggac cctgccgaag tgatcagggg ccacgatccg cagttggaga aagcgatcca
       61 ggtggccatg caacagctgg cagagcatcc ggtgacggag ccgacccggc cgcccttccc
      121 ggtgctggga aagaactgag tcttaaaatt gaataataat aatggctgaa ccaactattt
      181 taattaccgg cgctacggga ggccttggaa gcgtcgtcgt tccgtatttt ctcggcaagg
      241 gataccgcgt catcgccgtg gtttccgaag aaaacgcgaa gaacaggcct gaaagccacc
      301 ctttgctcga tgtacaagtt gtcgacctga ccgatgaagg ctctgtcggg tcgctgatcg
      361 catggctcat caggagatac ggcgcgatcg atggggcgct cctgctcgcc gggggatttg
      421 tcgccggtgg agtagccgaa gcgaccggga atgaggtcaa gacgatgttt gccatcaact
      481 ttgagacggc ctattacgta gtccgtcccg tttacaaaca catgttcgca aggggcaagg
      541 gaaggatcgt gctggtcggg gcgcggccgg cactggagcc ccaggcgggt aaggcttccg
      601 ttgcctatgc gttgagcaaa tcgttgttgt tccggctggc ggaaatgctc aacgcggacg
      661 cgggcgccaa agacgtggta acgcatgtgg tagtgccttc gaccatcgat acgccaaaca
      721 atcgcaaatc gatgccccat gcggatttcg acagctgggt caaaccggaa aagctggcgg
      781 agacctttta cctgatctgt tcgggatccg cggcgccttc gggcgacgcc gtttggaaaa
      841 tttacaataa tgcgtgacga gatggaaaat atcaagatcg caaccgcgca atttgaacac
      901 gcgagcggcg acaaggagaa gaacctaagg gtcatccggc ggatgacaga gcaggcagca
      961 accgcggggg cgcaggccat tgcttttcac gagtgcagca tcaccggata ctcttacgcc
     1021 cgatcgctga gcaaggccgg cctgttggcg gcggccgagt acattccgga aggcccaagc
     1081 gtccgggcac taacagaaat tgcgcggaat tcgggcgtgg tcgtcctcgc gggactgttc
     1141 gagaaagatg cggagaaccg gatctttaaa gcgtatgtgg cagtcggcgg atccgggctg
     1201 atcgccaaat tcaggaagct gcatcctttt attaatccgc atatcacgcc gggcaatggc
     1261 tatgtcgttt ttgatctttt cggctggaag tgcggcgtgc tcatctgcta tgacaacaat
     1321 gtcgtggaga atgtccgggc gactgccttg cttggcgccg acatcctctt tatgccgcat
     1381 gtcacgatgt gcacgccctc gacgcggccg ggcgccggat ttgtcgatcc ccggctttgg
     1441 gccgcgcggg agaccgatcc gacctcgctg cggatcgaat tcgacggcct gaaaggcagg
     1501 gaatggctca tgaaatggct gcccgcgcgg gcttatgaca acgggatata cgtcgtattt
     1561 tcaaacgcca tcggaatgga tgatgaccag ctcaagaacg ggtgttcgat gatcctcgat
     1621 ccgttcgggg atatcctcgc cgaatgccgg aatttaggga atcagctggt ggtggccacc
     1681 ctcacgccgg agaaactgcg gctggcgggc ggttatcgtt accggatggc aagaaggccc
     1741 gacctgtatc gggatatcat cggggcagcg cacgtgccgg aacaaaaagt cgtgtggatg
     1801 gatcagggta acgagtaatg accggcatcc taattttctc cgcagagaag aaattccgat
     1861 ttccgcggcc ggggacgctt gctccagtcg cgttgaagaa tttgtttcag cgcctcctga
     1921 aattccgttg aaaaatcgga cttcaggata aacaggtcgg ctccgccgtt gaaggcggct
     1981 tcaatgtccc gggttggacc ggcgaaagac tgtatgatga cgggaatgtg gttccattcg
     2041 gggttggagc ggatttcttc caggcattcg atcccggatt tgcccggcat attgacatcc
     2101 agaaagataa tatcgggaag tctgtcaacc ccagccagat aatacaacaa ctcgtcacta
     2161 tcggctaccc actccagctc gtgcctgatg ccgatgttcc gaagggtgaa cccaaaaagc
     2221 agggactctg cgccgttgtc atcgcagacg acgattttaa taggatcttg catggggcag
     2281 gtttacatca cactggcttt atagcataat ttataccaaa aaatccggaa aaaaaggccc
     2341 cggtacctat aatttttcca tgccgtcgcg ataagccttg ccaatgggaa tcgaatggcc
     2401 gtggatctca atcatttcgg cggtatacga ctcgatccgc cggatggcga cgatgtagga
     2461 gcgatgcacg cgcttaaaaa gaccaggcgg cagcaggccc tcgatctcgt ttgtgctcat
     2521 tttcgaaaga tattcgctgc cggtcgtcac taccctgata tattcccgct ggctttcaat
     2581 gtagacgatc tcggagaaaa ggatcttgac ccgttttttc tgaacggtga taaaaatgaa
     2641 gtcccgggct tcgggcccgg cggccggctg cgccgccggc gggtgggccg aacggacctt
     2701 gttaaccgcc gtcaggaacc ggtcaaactc gatcggtttc aggagataat cggtgacgct
     2761 tagattgaaa ccttcgacgg cgtattggtg gtaggccgtg gtaatgatta cggaaggtgg
     2821 atggggaagg gctttcagga aggcc
//
LOCUS       DATGKE010000562         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1355792, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000562 DATGKE010000000
VERSION     DATGKE010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355792"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VKU83_09560"
     CDS             complement(<1..616)
                     /locus_tag="VKU83_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLI93846.1"
                     /translation="MPIEKKLNILKEGLLLRSEHLDRTVQVDCYVPDELPAAQEVSLL
                     LINDGQDLGRMGLAGILADLYGRGVLSPLICAGIHAGAHRKMEYGTAGHANYQGWGVK
                     ADDYTRFLFDELLPLLGRRYGAPGFREKAFAGFSLGALSALDIVWRHPQEFTCAGLFS
                     GSFWWRKKDKDDPGFDEATDRIMHAAVRAGGYYPWLRFFFECGTK"
     gene            670..1395
                     /locus_tag="VKU83_09565"
     CDS             670..1395
                     /locus_tag="VKU83_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLI93847.1"
                     /translation="MDLGEQYHHWHSPVIGRSFDMLVFGHAGYPVILFPTSRGSYYQN
                     KDQGLIESARWLLENGKVKIYCPDSCDDSSWYNKSVPPAERARQHTLYDRLIFEEVLS
                     RALQETGHTRAALAGCSFGGYHAANFAFRHPAVAGYCFSMSGVFDIRSFTDGYYDDNI
                     YFNNPIDFIPGDKDPALWQMGIVLGTADRDICRSQNEAMSRLLAAKSISHWLDIRANR
                     DHDWAVWKEMFPHYLSLLPGAVP"
     gene            1433..2401
                     /locus_tag="VKU83_09570"
     CDS             1433..2401
                     /locus_tag="VKU83_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93848.1"
                     /translation="MSANKKIGILYGRERSFPMAFIEAINRRNIPGITAEPVRIDKVF
                     QAGDPGYSVIIDRISQDVPFYRAYLKNAALTGTAVINNPFWWSADEKFFNNCLAEKIG
                     VPVPKTVILPSRELPSDTTDQSFSNLAFPLDWDSIFHYVGFPAYMKPFAGGGWKNVYK
                     LHDAEEFYQRHGETGQLVMLLQEEIVFSEYYRCYCIGGKYVRVMPYEPRNPHHLRYSA
                     GFSPSAERLQQMEEIVLRINAWLGYDFNTVELAVRDGIPYAIDFCNPAPDAERTSVGE
                     DNFNWVVETSADYAIERALAHGEGVDNLTWGKYLGHSVAHQSLAME"
BASE COUNT          623 a          809 c          633 g          574 t
ORIGIN      
        1 ccttcgtccc gcattcgaaa aagaaccgca gccagggata gtacccgccg gcacgcaccg
       61 ccgcatgcat gatcctgtcc gtggcttcgt cgaagccggg atcatcctta tccttcttcc
      121 gccaccagaa gctgccgctg aaaagacccg cgcaggtgaa ctcctgcgga tgtcgccaaa
      181 cgatatccag tgcgctcagg gcgcccagac taaacccggc aaaagccttt tcccggaaac
      241 ccggagcccc gtaccgccgg ccgaggagag gtaaaagctc gtcgaatagg aaacgcgtat
      301 agtcatccgc ttttacgccc caaccctggt aattggcatg ccccgccgtg ccgtattcca
      361 tcttacgatg agcgcctgca tgaatgcctg cgcatatcaa cggggacaat accccccggc
      421 catacagatc cgccagtatc cccgccagtc ccatccgccc gaggtcctgc ccatcattga
      481 tcagcaacag actgacctcc tgcgcagccg gtagttcatc cggcacataa caatccacct
      541 gtaccgtacg gtcgagatgc tcggatcgca gtaataggcc ctccttcaaa atattcaatt
      601 tcttttcgat cggcatagcg ctaaaaaagt aagttgacca aagcgccaaa aatagtatat
      661 ttagcataaa tggatctggg agagcaatat catcattggc actcgccggt cattggccgg
      721 agttttgaca tgctggtatt cggacacgca ggctatcccg tcatcctctt ccctacttcc
      781 cggggttcct attaccagaa taaagaccag gggctcatcg agtcggcacg ctggctcctg
      841 gagaatggaa aagtaaagat ctactgcccc gacagctgcg acgattcctc ctggtacaac
      901 aaatcggtcc cgcctgccga acgcgcccgg caacatactt tatacgatcg cctgatcttc
      961 gaagaagtcc tgtcgcgcgc cctgcaggag accggccata cacgcgccgc actcgccggt
     1021 tgcagcttcg gcggctatca cgcagccaac ttcgccttcc gccaccctgc cgtggcaggc
     1081 tattgctttt ccatgagcgg cgtcttcgat atccggtctt ttaccgacgg ttattatgac
     1141 gacaacattt attttaataa ccccatcgat tttattccgg gcgataaaga tcccgcactc
     1201 tggcaaatgg gcatcgtcct cggaaccgcg gacagggata tctgccgtag ccagaacgaa
     1261 gcaatgagcc ggctgctcgc agccaaatcc atctcgcact ggctggatat ccgcgccaac
     1321 cgcgatcacg attgggccgt ctggaaagaa atgttcccgc actacctttc gttgctcccg
     1381 ggcgcagtcc cgtgaccggt atttattata gtaaacctca aatcgcattg caatgtccgc
     1441 caataaaaaa ataggaatcc tctatggccg ggaaagaagc ttccccatgg cctttatcga
     1501 agccatcaac cggaggaata tccccggcat cacggccgag cccgtccgca tcgacaaggt
     1561 cttccaggcc ggagatccgg gctatagtgt tatcatagac cgcatctccc aggacgtacc
     1621 cttttaccgc gcttatctca agaacgccgc gcttaccgga acggccgtga tcaacaatcc
     1681 cttttggtgg agcgcagacg aaaaattctt caataactgc ctggccgaaa agatcggcgt
     1741 tccggtaccc aagaccgtta tcctgccctc ccgggagctg ccgtccgata ccaccgacca
     1801 gtccttctcc aacctggctt tccctctcga ctgggattcg atctttcatt acgttggctt
     1861 ccccgcctat atgaaaccct tcgccggcgg tggttggaag aacgtgtata agctccacga
     1921 cgccgaagag ttctatcagc gccacggcga gacaggccag ctcgtcatgc tcctgcagga
     1981 agagatcgtt ttctccgaat actatcgctg ttattgcatc ggcggcaaat acgtgcgcgt
     2041 tatgccctat gagccccgta atccacacca cctgcgctat tccgccggct tcagcccctc
     2101 ggccgaacgc ctgcagcaaa tggaggagat cgtgctgcgt atcaatgcct ggctgggtta
     2161 tgacttcaat acggtggagc tcgccgtgcg cgacggcatc ccctatgcca tcgatttttg
     2221 caaccccgct cccgatgccg aaaggaccag cgttggagaa gacaacttca actgggtggt
     2281 ggaaacgtcc gccgactatg ccatcgaaag agccctcgcc cacggggaag gagtggataa
     2341 tcttacgtgg ggaaaatacc tcgggcacag cgtggcgcac caatcgctgg ctatggaatg
     2401 aattttgtgc aaataaattt ttttcggatg tccctttacc gtttaatttt accgattcaa
     2461 taaaacctaa attctatgta tacgatctat aacgaagaag aagaccgcgt gcaggaagtg
     2521 gaagaacccg attcctctct cacgtacacc tatgcggatt attatagctg gaaattcctc
     2581 gaaaggctcg aactgttgcg aggcaaggtc ttcaggctgt ccgcccccac taccaaaca
//
LOCUS       DATGKE010000563         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_442902, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000563 DATGKE010000000
VERSION     DATGKE010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442902"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2045
                     /locus_tag="VKU83_09575"
     CDS             <1..2045
                     /locus_tag="VKU83_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLI93849.1"
                     /translation="KVYQMMNSTPAIPRLHIPAYDWWNEALHGVARSGVATIFPEPIG
                     MAATFDDSLVHQVAAAISDEARAMYNASIKKNNRNRFSGLTFWTPNINIFRDPRWGRG
                     QETYGEDPYLTSRMGVALVRGLQGDDPRYLKTAACAKHYAVHSGPERLRHRFDAEVSM
                     HDLWNTYLPAFHALVNAKVEAVMCAYNRTNGEACCGNTYLLDDVLRRQWGFKGHIVSD
                     CDAIADIYDGHKLVSGKTGAAALALQRGVNLNCGDTYLSLLDAEKQGLVTENEIDSSL
                     AILLRTRFKLGLFDPAGSNPYDRIPESAINSAAHRALARKVAQKSIVLLKNNGALPLS
                     DGLKKYFLTGPNATSVDVLLGNYYGVNDRLVTMMEGLAAQVRPGSQLIYNQGVLLDRP
                     NVNPIDWASGNARGSDATIYVMGITGLLEGEEGESLASPTFGDRLDYNLPSAQIEYLR
                     KLKAGNSHPVIAVVTGGSPMNLAEVQQIADAVLLVWYPGEEGGDAVADILFGKVAPSG
                     KLPVTFPRSYDQLPAFDNYSMKGRTYRYMTAEPLYPFGFGLSYTRFTYSGLTVSSQKV
                     HRGETVSADVTLTNAGKFASDEVAELYLTHENGGDSAALFALKGFRRVSLAPGQSTRV
                     HFVLTPEQLSVVDDLGRLVEPEGKIRISVGGSLPTHRSEELGAARPVEGEIEIVGK"
     gene            2059..3231
                     /gene="uxuA"
                     /locus_tag="VKU83_09580"
     CDS             2059..3231
                     /gene="uxuA"
                     /locus_tag="VKU83_09580"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF003027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HLI93850.1"
                     /translation="MESAFLMQETMRWYGPGDPVSLWDIRQAGCTGVVTALHHIPVGQ
                     VWPPEEIAKRRAEVEAAGMRWTVIESLPVHEDIKKQKGDYERYIRNYQQSLRNVAREG
                     LEVVTYNFMPVLDWMRTDVSYEMPDRSKALRFEKLAFIAFDLFLLRRPGARKDYTAGE
                     IAEAQKRLDTMTTVEKETLYRNALLGLPGSEGRFTEEEILAALKSYEGIDAVKLKQHL
                     FYFLQQVVPVAEEEGLKLAIHPDDPPFPILGLPRIVSTERDAAELLDSAPSNANGICF
                     CTGSFGVRPDNDLPGMVRRLGDHIHFVHLRSTRRDEAGNFYEADHLGGDVDMVAVIRE
                     LLAVQQKRKISLPMRPDHGHQMLDDLSKTTYPGYSAIGRLRGLAELRGVQHGLAGR"
BASE COUNT          709 a          839 c         1012 g          680 t
ORIGIN      
        1 aaaaggtcta tcagatgatg aacagcacgc cggcgatccc gcgcctgcac attcctgctt
       61 atgactggtg gaacgaggcg ttgcacgggg tggcacggtc cggagtggca accatttttc
      121 cggagccgat cggtatggct gccacgtttg atgatagcct ggtgcatcag gtcgcggccg
      181 ccatttcgga cgaggcccgg gcgatgtata acgcatcgat aaaaaaaaac aatcgcaacc
      241 gctttagcgg cttgacattc tggaccccca acatcaatat cttccgtgac ccgcgctggg
      301 gacgcgggca ggagacttat ggcgaagatc cgtacctgac ctcccggatg ggcgttgcgc
      361 ttgtgcgtgg cctgcagggt gacgaccccc gctatctgaa gacggcggca tgcgcgaagc
      421 actatgccgt tcacagcggc cccgagaggc tacggcaccg cttcgatgcc gaggtctcga
      481 tgcatgatct gtggaacact tatctgccgg cgttccatgc gctggtcaat gcaaaggttg
      541 aggcggtgat gtgcgcgtat aaccgaacga atggcgaggc gtgctgcggg aacacctatc
      601 tgcttgacga cgttctgcgg cgtcaatggg gattcaaagg gcatatcgtg tcagattgcg
      661 acgccattgc ggacatttat gatggccaca aactggtgtc cggcaaaaca ggtgcagcgg
      721 cgcttgcatt gcaacgcggc gtcaatctca attgcggtga cacgtatctc tcgctgctcg
      781 atgcggagaa acagggcctg gtcacggaga acgagattga tagctcgctg gcaatcctgt
      841 tgcggacgag gttcaaactg ggcctcttcg accctgcggg cagcaaccct tatgaccgaa
      901 tacctgagtc ggcaattaat agtgccgcgc accgggcgct tgcgcggaag gtcgcgcaaa
      961 aatcgatcgt gctgttgaaa aataatggtg cccttcccct gtctgacggc ctgaaaaaat
     1021 actttttgac agggccgaat gcaacgagcg tggatgtcct tctcggcaat tattacggtg
     1081 tcaatgaccg cctggtgact atgatggaag ggttggcggc ccaggtgcgg cccggcagtc
     1141 aactgatcta caaccaggga gtgttgttgg accggccgaa cgtcaatccg atcgattggg
     1201 cgtccggtaa cgcaagggga tccgatgcca cgatctacgt catgggcatt acgggtctgc
     1261 tcgaaggaga agagggggag tcactcgcgt ccccgacctt cggcgaccgg ctggattata
     1321 acctgccttc cgcacagatc gaatatctcc gaaagctgaa ggccggaaac agtcatcctg
     1381 tcattgccgt ggttacaggc ggcagcccga tgaaccttgc cgaggtgcag cagatagccg
     1441 acgctgttct gctggtctgg tatccgggag aggaaggcgg cgacgccgtg gccgatatcc
     1501 tgtttggaaa agtcgcgccg tcaggcaagc ttccggtaac ctttcctcgc tcctacgatc
     1561 aacttcccgc attcgacaat tactctatga aggggaggac ctaccggtat atgactgctg
     1621 agcccctgta tcccttcggg tttggattaa gctacacccg tttcacgtac agcggactga
     1681 cggtgtccag ccaaaaggtt catcgcggcg agacggtcag tgcggatgtg acactgacca
     1741 acgccgggaa atttgcttcc gacgaggtgg ccgagcttta tctgactcat gaaaatggcg
     1801 gtgactcggc cgcattattt gcattgaaag gttttcgccg cgtatctttg gcgcccggtc
     1861 agtcgaccag ggtccatttc gtcctgacgc ccgagcagct gtccgtcgta gatgacctcg
     1921 ggcgccttgt ggagccggag ggcaaaatca ggatcagtgt ggggggatcg ttaccgacgc
     1981 accggagtga ggaactgggt gcggcccggc cggtggaagg agagatcgag attgtgggga
     2041 aataaaaccg atatattaat ggagagcgct ttcctgatgc aagagaccat gaggtggtat
     2101 ggacctggcg atccggtcag tctttgggac atccggcagg caggctgtac aggggtggta
     2161 acggccttgc atcatattcc cgttggccag gtgtggcctc cggaggaaat tgcgaaacga
     2221 cgggccgagg tggaagccgc gggcatgcgg tggacagtga tcgaaagcct gcctgttcat
     2281 gaagacatca agaagcagaa gggcgattat gagcgataca tccgcaatta ccagcaaagc
     2341 ctgagaaatg ttgccaggga ggggctcgag gtggtgacct acaattttat gccagtcctc
     2401 gactggatgc gaacggatgt ctcatacgag atgcccgatc gcagtaaggc tttgcggttt
     2461 gaaaagttgg ctttcattgc attcgatctc tttttgcttc gccggcccgg cgcccggaag
     2521 gattataccg ccggcgaaat cgctgaggcg cagaagcggc ttgacacgat gacaacagtg
     2581 gagaaggaaa cgctgtaccg gaatgctttg ctgggtttgc cgggaagcga ggggcggttc
     2641 acggaagaag agatcctggc ggcgctgaaa tcctatgagg gcatcgatgc ggtcaaactg
     2701 aaacagcact tattctattt tttgcaacag gttgtcccgg tggctgagga ggagggactc
     2761 aaactggcta tccatcccga cgatccgcca tttccgatcc tggggcttcc ccgaatcgta
     2821 agcaccgaaa gggacgcagc agagctactg gattctgcgc catcaaatgc taatgggatt
     2881 tgcttctgca ccggatcgtt cggggtgaga cccgacaacg atctgcctgg aatggtgcgg
     2941 cgtttgggcg accatatcca tttcgtccac ctacgaagta cccggcgaga cgaggcgggc
     3001 aacttttatg aggccgacca ccttggggga gacgtcgata tggttgcggt catccgggaa
     3061 ttgctggccg tccagcaaaa gaggaagatt tcgcttccga tgaggcctga tcacgggcat
     3121 cagatgctgg atgacctctc aaagacgaca tatcccgggt atagcgcaat tggccggctc
     3181 cgcgggctgg ccgagttgcg gggtgtacag catggcctgg cgggtcggtg atcaccgcag
//
LOCUS       DATGKE010000564         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_710930, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000564 DATGKE010000000
VERSION     DATGKE010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710930"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VKU83_09585"
     CDS             <1..1463
                     /locus_tag="VKU83_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLI93851.1"
                     /translation="QGSLGGILPWGNVGDWNQYHCQNYDYYGNNIYSWAAVNSSFDPY
                     LVMQNVKQMGQEALSRGAAAVNPYEAVGRFVKAYYYYNLTSMFGDVPLNDALEGPQNT
                     TPAYTAQEQVFAYILNQLDSANNDLASLIAANDNSLSATQDIYYGGNLSAWQKAVNSF
                     RLRVLVSLSAQAADATLGVPAQFANIIGNPGKYPVFSGPGDDLQFVFNPGEANTYSTY
                     PFNPSNFGSIAGRYNMAYTYVNALTTINDPRVFMTCDPAWALVTNVDSPAQYAYFVGA
                     STGLDLGQMYSNASAGDYSYIGRYRYYSNFTGDPDVLVGYKEMCFNIAEAIERGWVTG
                     NAETWYQAGIKASMAFYGINLNQTKQTAYFLPPGANSVTQVAPYNYNFDWNAWYSQPA
                     VQLSSTPATAINQIVLQKYIVCFENSGYEGYYNWRRTGVPAFQGGSGVGNNGIVPLRW
                     AYPIGEQTQNAANWKAAVANQGFSADDLNQTMWLIK"
     gene            1504..2085
                     /locus_tag="VKU83_09590"
     CDS             1504..2085
                     /locus_tag="VKU83_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLI93852.1"
                     /translation="MTTEQAREISDGIIRLYEEHGGAEYAGEKVSQLEHMVQAAQLAE
                     AQGFDEEVILAAFLHDIGHISEAAKGDNEMDGFGIRDHEELGAQFLREKGFSKKVTRL
                     VESHVEAKRYLTYKDPSYYTNLSEASKRTLEYQGGPMTEGEATAFEQYPLFSLIIRMR
                     NWDEQAKIEQQPIPDLRRYREMMIHHLQTAGTR"
     gene            2105..2797
                     /locus_tag="VKU83_09595"
     CDS             2105..2797
                     /locus_tag="VKU83_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLI93853.1"
                     /translation="MSAFDLVVFDIAGTTVRDRGNVADAFIEAFADYELRIPAGDVKK
                     VMGFRKMDAIVLLLEKFAPASKDDGVLVDRIHTRFIQKMIEFYRNDDTLAPLPHAEDV
                     FRTLKQRGVKVALNTGFTRSITETILHRLRWDDRCPLIDQVICSDEVPHGRPYPDMIK
                     VLMDSLGVRSAERVLKVGDTGVDVEEGRNAGCGKVVAVTTGAYTRKQLEPYKPDYIID
                     DLREILPLVEQE"
     gene            2807..>3680
                     /locus_tag="VKU83_09600"
     CDS             2807..>3680
                     /locus_tag="VKU83_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039829.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5690 family protein"
                     /protein_id="HLI93854.1"
                     /translation="MSKGVRHPADIPPGQTLDLPAGPAAGSATGSPADSPAGSPADSA
                     AGDPAFGHSVRRTGSLRSWLGRCPGWVFVIYVSLTSFTVYACMYGFRKPFTAASYDGV
                     RFLGISYKVVLVIAQVLGYMLSKFYGIRFIAGMQPARRAGFIVRLVLTAWVALLLFGI
                     VPAPYNFVFMFINGLPLGMVWGLVFGFLEGRRTTELMGAVLATSFIFASGLAKTVGKS
                     LMLSAGISETWMPFAAGAVFIGPLLISTWLLNQTPAPNAEDIAHRTIRLPMGKEQRQN
                     FLRTFGIALIPVVLA"
BASE COUNT          785 a         1079 c         1077 g          739 t
ORIGIN      
        1 cgcagggtag cctcggaggc atccttccct ggggaaatgt aggcgactgg aatcagtacc
       61 actgccagaa ctacgattat tatggcaaca atatttattc ctgggcggcc gtcaactctt
      121 ctttcgaccc ctatctcgtg atgcagaacg tcaagcaaat gggccaggaa gcgctgagcc
      181 gcggcgcggc ggccgtcaac ccctacgaag cggtcggccg tttcgtcaaa gcctattact
      241 actacaacct gacctcgatg ttcggcgacg ttcccctgaa cgatgcgctg gaagggccgc
      301 aaaacacgac gccggcctac accgcgcagg agcaggtgtt tgcctatatc ctcaatcagc
      361 tggattcagc caacaatgac ctggcttcgc tgattgccgc caatgacaac tccctctccg
      421 caacccagga catttattac ggcggcaacc ttagcgcctg gcaaaaagcg gtgaacagct
      481 tccgcctgcg ggtgctcgtc agtctgagcg cccaggccgc ggatgcgacg ctgggagttc
      541 cggcccaatt tgcaaacata atcggcaacc ccggcaagta tccggtcttc agcggcccgg
      601 gggatgacct ccagtttgta ttcaatccgg gcgaggcgaa cacgtacagc acgtatccgt
      661 tcaatccgag caatttcggt tcgatcgccg gacgttacaa catggcgtac acttacgtga
      721 acgccttgac gacgatcaac gacccgcggg tcttcatgac ctgtgacccg gcctgggccc
      781 ttgtgacgaa cgtcgactcg ccggcccagt atgcctactt cgtaggcgcc agtaccggcc
      841 ttgacctggg ccagatgtac agcaacgcca gcgcaggcga ttattcctat atcggccggt
      901 accggtacta ctcgaatttt acgggcgatc ccgacgtgct ggtcggctac aaggaaatgt
      961 gtttcaacat cgccgaagcg atcgaacgcg gctgggtcac cgggaatgcc gaaacctggt
     1021 atcaggcggg catcaaggcg tcgatggcct tctacggcat caatctgaac cagacaaagc
     1081 aaaccgccta cttccttccg ccgggcgcca attcggtcac ccaggtagcg ccgtataact
     1141 acaacttcga ctggaatgcc tggtactctc agccggccgt ccaactctcg tcgacgcccg
     1201 ctaccgcgat caaccagatc gtcctgcaga aatatattgt ctgcttcgag aattccggct
     1261 acgagggcta ttataactgg cgtcgcaccg gcgtcccggc attccagggc ggcagcggcg
     1321 tggggaataa cgggatcgtg ccgctgcgat gggcttaccc gataggcgag cagacccaaa
     1381 atgcggcgaa ctggaaagcg gcggttgcca accaggggtt cagcgccgac gacctgaacc
     1441 agaccatgtg gctgatcaaa taaggaggaa gaattgtatc ttgtccattc taatacatca
     1501 cgcatgacca cagagcaagc ccgggagatc agcgatggga tcatccgcct gtatgaggaa
     1561 catggcggcg cggaatatgc cggtgagaag gtgtcgcagc tcgagcatat ggtgcaggcc
     1621 gcccagctgg ccgaggccca gggtttcgat gaagaagtta tcctggcggc tttcctgcac
     1681 gatatcggtc atatcagcga ggcggccaaa ggcgataatg agatggatgg gttcgggatc
     1741 agggaccacg aagagctggg cgctcaattt ctgcgggaaa aaggcttttc gaagaaggtc
     1801 acccggcttg tggagtcgca cgtggaagcg aaacgatatc ttacctacaa agacccttcg
     1861 tattacacga acctttccga agcgagcaag cggacgctcg aataccaggg cggcccgatg
     1921 acggaggggg aggcgacggc attcgagcaa tatccgctct tcagcctcat catccggatg
     1981 cgcaactggg atgagcaggc caagattgaa cagcagccca ttcccgacct gcgccggtac
     2041 cgggagatga tgatccatca cctgcaaacc gcgggaacaa ggtaatgaac ccaaccgtca
     2101 aatcatgagc gctttcgacc tggttgtttt cgacatcgcc ggcactacgg tgcgcgacag
     2161 gggaaatgtc gccgatgctt ttatcgaagc gttcgcggac tatgagctgc gaatcccggc
     2221 cggggacgta aaaaaagtga tgggtttccg gaagatggat gcgattgtcc tgctcctgga
     2281 aaaatttgcc ccggccagca aggatgacgg ggtgctggtc gaccggatcc atacccggtt
     2341 tatccagaag atgatcgaat tttaccggaa cgacgatacg ctggcgccgc tgccacatgc
     2401 ggaagacgtg ttcaggaccc tcaagcagcg gggcgtcaag gtcgccctca atactgggtt
     2461 cacccgttcc atcaccgaaa cgatcctcca ccggctgcgt tgggacgaca gatgcccgct
     2521 catcgaccag gtgatctgca gtgatgaggt gccgcatggc cgcccatatc ccgacatgat
     2581 caaagtgctg atggattcgc ttggagtccg gtcggcggaa cgggtcctca aggtaggcga
     2641 cacaggggtc gacgtggaag agggccggaa cgcgggttgc ggaaaagtcg tggccgtcac
     2701 gacgggggct tacacgcgca agcaactcga accttacaag cccgattaca tcatcgacga
     2761 tctgcgggag atccttccgc tggttgaaca ggaataaaac agcaatatgt ccaaaggggt
     2821 cagacaccca gccgatatcc ctcccggtca gaccctcgac ttgccggccg gccctgcagc
     2881 ggggtccgcg actggctccc cggcggactc cccggctggc tccccggcgg actccgcggc
     2941 cggcgacccg gcgttcggcc attcggtgcg tcgcaccggc agcttgcggt cgtggctcgg
     3001 ccgctgcccg ggatgggtct tcgtcatcta cgtgtcgctg acgtctttca ccgtctatgc
     3061 ctgcatgtat ggtttccgca aaccgtttac cgcggcttcc tatgacggcg tccggttcct
     3121 gggcatttcc tacaaggtgg tcctggtgat cgcgcaggta ctggggtata tgctcagcaa
     3181 gttttacggc attcgtttta ttgccggcat gcaacccgcc cggcgggctg gtttcatcgt
     3241 cagactcgtg ctgaccgcgt gggtggcgct gttattgttt gggatcgtac ccgccccata
     3301 caattttgtc ttcatgttca tcaatggact gccgctagga atggtttggg gcctggtctt
     3361 tggcttcctg gaaggacgaa ggacgaccga gcttatgggc gccgtgctgg ccaccagctt
     3421 tatctttgcc tcgggacttg ccaagacggt aggtaaaagc ctgatgctct ccgcgggcat
     3481 cagcgagacc tggatgcctt ttgccgccgg ggccgtgttc atcgggccgc tcctgatcag
     3541 cacctggttg ttaaaccaaa cgccggcgcc gaacgcggaa gacattgcgc atcgcaccat
     3601 ccgcctgccc atgggaaagg agcaacggca gaattttctg cggacatttg gaattgccct
     3661 catccctgtc gtcctggcct
//
LOCUS       DATGKE010000565         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_340911, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000565 DATGKE010000000
VERSION     DATGKE010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340911"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VKU83_09605"
     CDS             <1..1165
                     /locus_tag="VKU83_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI93855.1"
                     /translation="LRQRSCWITYTNQTVHDILKTGFDRSPMFTGRIEGVGPRYCPSI
                     EDKINRFAERERHQLFIEPEGWNTIEIYVNGFSTSLPEEVQVAALRQVPGFENVRIFR
                     PGYAIEYDYFPPTQLRSSLETKALNHLFFAGQINGTTGYEEAACQGLMAGINAHQKIR
                     QLEPLILKRSEAYIGVLIDDLISKGTDEPYRMFTSRAEYRTLLRQDNADLRLTELSFR
                     LGLASQERMERTRAKIAAISAIKSILHQASIDPEETADYLLERKTSPLTQKQRAAQVL
                     LRPDVDLISLARAVPQLSKAFDAYDAETLEQAEIQIKYDVYINKERELVEKMAQLEDL
                     LIPENFNYDRLSSLSAEARQKFKKVRPRTLGQASRISGVNPSDVQILLVYMGR"
     gene            complement(1199..2194)
                     /gene="nadA"
                     /locus_tag="VKU83_09610"
     CDS             complement(1199..2194)
                     /gene="nadA"
                     /locus_tag="VKU83_09610"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLI93856.1"
                     /translation="MQDINTSISAKGFLDIPVSPELDLFLEIERLKREKSAVVLAHYY
                     QESDIQDVGDYIGDSLGLAQRAAKTSANLIVFAGVHFMAETAKILNPDKKVVLPDLRA
                     GCSLADSAPGELFKAFKEQHPDHIVISYINCSAEVKALSDLICTSSNAEKVVESVPPD
                     RKIIFAPDRNLGAYLIKKTGRDMVLWNGACMVHEIFSVEKITRLKIRHPEAKLIAHPE
                     CEEPILRMADFIGSTTGLLNFTRKDSGRQYIVATETGILHQMQLSSPDKLFIPAPPTT
                     NCACNDCPHMKLNTLEKLYLCMEYEQPELVMDKGLMEAARRPIERMLEISAKAGL"
     gene            2633..>3496
                     /locus_tag="VKU83_09615"
     CDS             2633..>3496
                     /locus_tag="VKU83_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLI93857.1"
                     /translation="MSIIQQLRERAGWLLGGLIALSLIGFLLMDARSSRFFGGGGRTT
                     VVGVVNGHDIEADQYNAEVNQIEDRYKAQNNGGVDDALREQIREEVWNQMVQEALLNK
                     DYSALGMEISDKEESDMLVGQDAIPDIKRAFTDPKTGMFDAQAAAQQINEIRRIYTAG
                     PRRNANGDNRQYEMARAFWEEEVPQYVKSRLEQKFITLLSNSAYVPKWMAEKTIADNS
                     QIAALSYVEDPYFTIPDSAAKVSDEEITDYVKQHPTEFKQEESRSIAYVTFNAGPTSA
                     DSARVRQQLMDL"
BASE COUNT          866 a          979 c          882 g          769 t
ORIGIN      
        1 gctccgccag cggagctgtt ggatcaccta tacgaatcag accgtccatg acatcctgaa
       61 aaccggcttt gaccgcagcc ccatgttcac cggtcgcatc gaaggcgtcg ggccgcgcta
      121 ctgtccgagt attgaagaca agatcaatcg cttcgccgaa cgcgaacgcc accagctctt
      181 catcgagccg gaaggctgga acaccatcga aatctatgtt aatggatttt cgacttccct
      241 tcccgaggaa gtgcaggtcg ccgcgcttcg gcaggtcccg ggttttgaaa atgtccggat
      301 cttcaggccc gggtatgcca ttgaatatga ttatttcccg ccaacccagc ttcgatcctc
      361 gcttgaaaca aaggcgctca accatctctt tttcgctggc cagatcaacg ggacaaccgg
      421 ctatgaagag gccgcttgtc agggactgat ggccggcatc aacgctcatc aaaagatccg
      481 tcagcttgag ccgctgatcc tcaagcgcag cgaagcctat atcggcgtac tcatcgacga
      541 cctcatcagc aaagggacgg acgaacctta ccggatgttc acctctaggg ccgagtatcg
      601 aaccctttta cgccaggaca acgccgatct caggttaaca gaattgagct tccggctcgg
      661 cctggcctcg caagaaagaa tggaacgcac ccgggcaaag attgctgcca tttccgctat
      721 caaatcgatc ctccaccagg caagtatcga cccggaagag acagcggact acctcctcga
      781 aagaaaaacc agcccgctca cccaaaagca acgcgccgcc caggttttgc ttcggcccga
      841 cgtcgacctg atttccctgg cccgggccgt cccgcaactt agcaaggcgt tcgacgccta
      901 tgatgccgaa acgctggaac aggcagagat ccagatcaaa tacgacgtgt acatcaacaa
      961 agaacgggag ctggtagaaa aaatggcgca gctggaagac ctcctcattc cggaaaattt
     1021 taactacgac cgattatcgt ccctttcagc agaggcccgg caaaagttca aaaaggtcag
     1081 gcccagaacc cttggacagg ccagccgcat cagcggtgtt aaccccagcg atgtccagat
     1141 cttactggtc tatatgggta gatgattgat tattaataag ttaaaagagt ttctctgctc
     1201 aaaggccggc ctttgcactg atctccagca tccgctctat cggcctgcgc gccgcctcca
     1261 tcagtccctt atccatcacc agttccggct gctcatactc catgcacaga tacagcttct
     1321 cgagcgtatt tagcttcatg tgcggacaat cattgcaggc gcaattagtg gtcgggggag
     1381 cggggataaa cagcttatcg ggggacgaca gctgcatctg atgcaagatc ccggtctcgg
     1441 tcgccacgat gtattgccgg ccgctatcct tccgcgtgaa gttgagaagc ccggttgtcg
     1501 agccgataaa atccgccatc cgcaggatcg gctcttcaca ttcgggatgg gcgatcaatt
     1561 tggcctcggg atgccggatc ttcaggcgcg ttatcttctc cacgctgaag atctcatgaa
     1621 ccatgcaggc gccattccat aacaccatat cccggccggt ctttttgatc agataagcgc
     1681 cgagattccg gtccggcgcg aagataatct tccggtcggg cggcacgctc tcaaccactt
     1741 tctccgcatt cgaagaagtg cagataaggt cactcagggc cttcacttcc gccgagcaat
     1801 tgatatagga aatgacgata tggtccggat gctgttcctt gaacgcttta aacaactctc
     1861 ccggcgccga atccgccagc gagcaacccg ccctcagatc gggcaaaacc accttcttgt
     1921 ccgggttcag gatcttcgcc gtttcggcca taaaatgcac gcccgcgaaa acgatcaggt
     1981 ttgccgaggt ctttgcagcc cgctgcgcca ggcccaggct atcgccgata taatccccca
     2041 catcctggat atcggactcc tggtaataat gtgcaagcac gacggcactt ttttcccgct
     2101 tcagccgttc tatttccaga aaaagatcaa gctccgggga taccgggata tcaaggaacc
     2161 ccttcgccga aatgctggtg tttatatctt gcatagtatt taataatatc ctttattttt
     2221 taaaaagaac tactactatt agccgcgtgg attagtggaa aagaaaattc tccagctatt
     2281 tgatttacaa aaatcacttg tttatacacg gtgatccaca tcttgctaac aaccggcaac
     2341 cgcgttttga ttggtcttcc ggtgcttttc cacagcggtc gatgataagc ccatgagcat
     2401 aaattgtttg accgggggtt gtcagcgggt gtggaataag tcctgctttc tccacggcat
     2461 cctgccagcc ggcggcacgc cagcgactta tcatccattt cctcccaggt ccggtttgcc
     2521 gaaaaccccg gttttccccg cctaaacagc cttttccaca atttgttcat aagcgttttt
     2581 tggcctattt tcgccctccg tttgaccgaa aatacaacgg agaatcaaca ttatgtcaat
     2641 tattcagcaa ctacgcgaga gagcaggatg gctattgggt ggcttgatcg cgctaagtct
     2701 tattgggttt ttattgatgg atgcaagaag cagccgcttt tttggcggcg gcggccggac
     2761 tacggtcgtt ggggtcgtca atggacatga tatcgaggcg gaccaatata acgcagaggt
     2821 taaccagata gaggacaggt ataaggcaca gaacaacggc ggcgtggatg atgccctgag
     2881 ggagcagatc cgcgaagagg tttggaatca aatggttcag gaggccctcc tcaataaaga
     2941 ttattccgcc ctgggaatgg agatcagcga caaggaagag agcgatatgc tggtagggca
     3001 ggacgccatt ccggatatca aacgcgcgtt caccgatccc aagaccggca tgttcgatgc
     3061 ccaggcagcc gcccagcaga tcaacgagat ccgcaggatt tacacggccg ggccgaggag
     3121 aaacgcgaat ggcgacaacc ggcagtacga aatggcgagg gcattctggg aagaagaggt
     3181 ccctcaatat gtcaagagcc gcctcgagca gaagttcatc accctgttgt ccaatagcgc
     3241 ttacgttccg aaatggatgg cggaaaaaac gatcgccgac aacagtcaga tcgctgcgct
     3301 ttcttatgtg gaagatccgt atttcacgat tccggatagc gccgcgaaag taagcgatga
     3361 agagattacc gactacgtga agcagcaccc cacggaattc aaacaggagg aaagccgcag
     3421 catcgcctat gtcactttca atgccggccc cacttccgcc gacagtgcgc gggttcgcca
     3481 acaactgatg gacctt
//
LOCUS       DATGKE010000566         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_651578, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000566 DATGKE010000000
VERSION     DATGKE010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651578"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VKU83_09620"
     CDS             <1..201
                     /locus_tag="VKU83_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93858.1"
                     /translation="GEIIDDGVGKLLPLDITPEALASLLQDYHAESPARKTELRKAAF
                     ERYREKCDAEVLARDFAKMAIQ"
     gene            217..831
                     /gene="hisH"
                     /locus_tag="VKU83_09625"
     CDS             217..831
                     /gene="hisH"
                     /locus_tag="VKU83_09625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLI93859.1"
                     /translation="MIAILDYGIGNVSSIKNMFKKVGVAATITSDPAAIGSAEKIILP
                     GVGNFDYCMKQLRNAPFFELFTRRALEEKVPVLGVCVGCQMLMESSEEGNEKGLGWIR
                     GKVVRFDSSKASEPIRIPHMGWTDVRPKENKGIYAGTQDPRYYFVHSYHLVCDDPRDI
                     SATARYGYEFVASVEHGSVTGVQFHPEKSHKFGMQLYKNFVNEY"
     gene            841..1611
                     /locus_tag="VKU83_09630"
     CDS             841..1611
                     /locus_tag="VKU83_09630"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HLI93860.1"
                     /translation="MRRIRVIPVLLIKGDGLYKSQYFKNYRYVGDPINAVKIFNDKEV
                     DELAIIDISATKEKRPPNIRKIAEIASEAFMPLSYGGGITTIDEVREILFNGIEKVII
                     NKAAVARPGLITEISNRYGAQSVTVSIDVKKNWLGKYRVYTENGTQNTDLGPAAFARQ
                     CEDRGAGEILLNSIDRDGGYKGYDLEILQQVSNAVTIPVIAVGGAGAVEDFREAVEKG
                     KASAVAAGSMFVFRRPHNAVLISYPSQSELREKLYAND"
     gene            1604..>2023
                     /locus_tag="VKU83_09635"
     CDS             1604..>2023
                     /locus_tag="VKU83_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93861.1"
                     /translation="MTNMDVREISGIRWYDEEKLKADRAANNGRPYQQCAISVMDTIA
                     DPNISFDASGICNYYHQYKKLEKEEVFTGEAGRKRLDETVAQIKASGKGREYDCILGL
                     SGGADSSYLAYLAKQQGLRPLVVHFDYGWNLELAVQNI"
BASE COUNT          527 a          510 c          593 g          393 t
ORIGIN      
        1 ggagagatca tcgacgacgg cgtcggcaag ctgctgccgc tggacattac gcccgaagcg
       61 ctcgcctcgc tcctgcagga ttatcatgcg gaaagccccg cacggaagac ggagctgaga
      121 aaggctgcat tcgagcggta ccgggagaaa tgcgatgccg aggtgctggc cagggatttc
      181 gcaaagatgg ctattcaatg acagggaact aattgtatga tcgcaatttt ggattacggg
      241 atagggaatg tttcttccat caagaatatg ttcaagaagg tcggcgtggc cgccacgatt
      301 accagcgacc ccgctgcgat cggttcggcc gaaaagatca tcctgcccgg tgtgggaaac
      361 ttcgactatt gcatgaagca gctccgcaac gccccatttt tcgagctctt cacccgccgg
      421 gccctggagg aaaaggtgcc ggtcctgggc gtttgcgtcg gctgccagat gcttatggaa
      481 tccagcgaag aaggcaacga aaagggattg ggatggatca ggggtaaggt ggtccgtttc
      541 gatagcagca aagcgagcga gccgatccgt atcccccata tgggttggac cgacgtgcgg
      601 cctaaggaga acaagggcat ttatgcaggt acacaggatc cgcggtatta ttttgtgcat
      661 tcctatcatc tcgtttgcga tgacccccgg gacatatccg cgactgcccg gtacggctat
      721 gagttcgtcg catctgtgga acacggttcc gtcaccggcg tacagttcca ccccgaaaaa
      781 agccataagt tcgggatgca gctgtataaa aacttcgtga atgaatacta atctgcccat
      841 atgagacgta tccgcgtcat accggtcctg ctcataaaag gcgacggtct ttataaatcg
      901 cagtatttta agaattaccg ctacgtgggc gatcccatca atgcggtaaa gatctttaac
      961 gacaaagagg tagatgaact cgccatcatc gatatcagcg ctacaaagga aaagcggccg
     1021 cccaatatcc gcaagatcgc ggagatagcc agtgaggcgt tcatgccgct cagctatggc
     1081 ggcgggatca cgaccatcga cgaagtaagg gagatactct tcaacggtat cgaaaaggtg
     1141 atcatcaata aagcggcggt cgcaaggccc ggactcatca ccgagatcag caaccgctat
     1201 ggggcacaga gcgtaactgt cagcatcgac gtcaaaaaga actggcttgg gaagtaccgg
     1261 gtctatacgg agaacggcac gcagaacacc gatctcgggc cggcggcttt tgccaggcaa
     1321 tgcgaggacc ggggcgcagg cgagatcctg ctgaattcga tcgataggga tggcggctac
     1381 aaaggatacg acctggagat cctgcagcaa gtgagcaatg ccgtcactat tcctgtgatc
     1441 gccgtgggcg gcgcgggcgc agtggaagat ttcagggaag cagtggagaa agggaaggca
     1501 tccgccgtgg ccgccggcag catgttcgtt ttccggcggc cgcacaacgc cgtattgata
     1561 agctaccctt cccaatcgga gctgagggaa aaactatatg ccaatgacta acatggacgt
     1621 acgagagatc agcggcatac gctggtacga cgaggaaaag ctaaaggccg atagggctgc
     1681 caacaacggc aggccctatc agcaatgcgc catcagtgta atggacacca ttgcagaccc
     1741 caatataagt ttcgatgcat cggggatctg taattattat catcaataca agaagctgga
     1801 aaaagaagag gtctttacgg gcgaagcggg ccggaagaga ctggatgaaa ccgttgccca
     1861 aataaaggcc agcgggaaag gcagggaata cgattgcatc ctggggctga gcggcggtgc
     1921 agacagctcc tatctggctt atctggccaa acaacagggt ctgcggcccc tcgtggtcca
     1981 tttcgactat ggctggaacc tggaactcgc cgttcagaac atc
//
LOCUS       DATGKE010000567         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_236578, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000567 DATGKE010000000
VERSION     DATGKE010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236578"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VKU83_09640"
     CDS             complement(<1..631)
                     /locus_tag="VKU83_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI93862.1"
                     /translation="MNKALRTVIAFSLKNRYFILFCTGLLLITGIITFRNMPIEAFPD
                     VTNTEISIITQWPGRSAEEIEKFVTIPVEIAMNPVQQKISLRSTSIFGLSYVKLIFED
                     GVVDKDARAQVNALLQNVTLPEGIQPQVQPPTGPTGEIYRYTLQSKLRDDRELKTMQD
                     WIIDRQLRSVPGVGDIVSFGGRVKTYEIQVDPGKLTNLGITPLDVFNAVG"
     gene            complement(662..1768)
                     /locus_tag="VKU83_09645"
     CDS             complement(662..1768)
                     /locus_tag="VKU83_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLI93863.1"
                     /translation="MQNLTKTGRPLLALLLASGLMLLQSCEEKDKKPEPEKGYVIPDT
                     VMRTLKIDTVGMSQLVNAVTLTGKVGTNDDRVVPVYSLVSGVVSDIKVLLGDFVTAGE
                     TLAVVRSQEMAQYSSDLLNAQTNLAIAQKNLEKTVDMYHSGLASVPDSLAAAVQLQQA
                     KAELDRVNRVLKINGGNTQGEFVVRAPISGFIVQKGATNDMTIRGDNSTSLFTISDLK
                     DVWIQANVYESNISLIHQGENVDVSTIAYPGKIFKGKIDKVMNVLDPASKVMKVRVVL
                     PNPGYLLKPEMYASITVTEKENKQCLSVPSSAIIFDHSQNFVLLYHSPSDVKITPVQV
                     VSAVGDKTFISAGVAEGDKVVASQAILIYNALNS"
     gene            complement(1789..>2541)
                     /locus_tag="VKU83_09650"
     CDS             complement(1789..>2541)
                     /locus_tag="VKU83_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI93864.1"
                     /translation="GYIAEKEVVRIRAQLYSFQAEYSDLINSINSTESELRLVLQARP
                     NVFVDPVVDSSAIDKLDPGQYSLNTLLDSAYRNRTDLQIARTNTKINQLNYDYQRALA
                     VPDPSLSLGYDEAGSFLYGYYGIGVSIDLPVFNHNQGNIRNAKSLIDNTAATQKFTQA
                     TVEENVAVSLQKAFAQERLFRSIDPKFAANFERLTGEVIKNYEKRNISILDFLDFYDS
                     YKQNAVELNTILFNRVSAFEDINYYTGTNFFN"
BASE COUNT          506 a          654 c          747 g          634 t
ORIGIN      
        1 tgcccacggc gttgaagacg tccagtggcg tgatgcccaa attggtcagc ttccccgggt
       61 caacctggat ctcgtatgtc ttgacgcgac cgccgaagct gacaatatcc ccgacgccgg
      121 gaaccgaacg aagctggcgg tcgatgatcc agtcctgcat cgtcttcagc tcgcggtcgt
      181 cccgcagctt gctttgcagg gtgtaccggt aaatttcgcc ggtggggccg gtcggcggct
      241 ggacctgcgg ctgaatgcct tcgggtaagg taacgttctg gagcaacgca tttacctgcg
      301 cccgggcgtc cttgtcgaca acaccgtctt caaagatcag ttttacatag gaaaggccga
      361 agatgctggt agaccggagg ctgatctttt gttgcacggg gttcatggcg atctcgacag
      421 ggatcgtcac gaatttctcg atctcttcgg cgctgcgtcc gggccactgc gtaatgatgc
      481 tgatctcggt attggtaacg tccggaaaag cctcgatcgg catgttccgg aacgtgatga
      541 taccggttat gagcagcaga ccggtgcaaa acaaaatgaa gtacctgttc tttaaagaaa
      601 aggcgatgac ggttctgaga gccttgttca tctccgcacg gttttaatat gtagaatgcc
      661 tttagctgtt cagtgcattg taaataagaa tggcctgcga ggcgacgacc ttgtcgcctt
      721 cggccacccc tgctgaaata aaggtcttat cgccgacggc gctgaccacc tgcacggggg
      781 tgatcttgac gtccgatggg ctatggtaga ggagtacaaa gttctggctg tgatcgaaaa
      841 taatggcgga ggacggcacg ctgaggcact gcttgttttc cttctccgtc acggtgatac
      901 tggcgtacat ctccggcttg agcaaatagc cgggattcgg caggacgacc cggaccttca
      961 ttaccttgct tgccggatcg agcacgttca tgactttatc gatcttgccc ttgaatatct
     1021 ttccggggta ggcgatggtc gagacgtcga cattttctcc ctgatggatg agcgaaatat
     1081 tggactcgta gacgttggcc tggatccata cgtccttcag gtcggagatc gtgaaaaggc
     1141 tggtgctgtt gtcgccgcgg atggtcatgt cgttggtagc ccctttctgc acgataaagc
     1201 cgctgatcgg cgcccggaca acgaactcgc cctgggtatt gccgccattg atcttcagca
     1261 cacggttgac gcggtcgagt tctgctttcg cctgctgcaa ctgcaccgcg gccgcgaggg
     1321 aatccggtac ggatgccagt ccgctgtggt acatgtcgac ggtcttttcg aggttctttt
     1381 gggcaattgc gaggttggtc tgcgcattga gcaggtcgct gctgtactgg gccatctcct
     1441 ggctgcggac gacggcaagg gtctcgcctg cggtgacgaa atccccgagc aggactttga
     1501 tgtcggatac tacgccgcta accagggaat atacgggcac cacgcggtcg tcattggttc
     1561 ctaccttccc ggtcagcgtg acagcattga ccagttggga catgccgacc gtgtcgatct
     1621 tgagcgtccg catgacggtg tcgggaatca cgtatccctt ttccggctcc ggcttcttat
     1681 ccttttcctc gcaggactgt agaagcatca ggcccgatgc gaggaggagg gcgagaagcg
     1741 gccggccggt cttggtgagg ttttgcatat gaatggatgt tttcccgatt agttaaagaa
     1801 gtttgtcccg gtataatagt tgatatcctc gaacgcgctg acgcggttga acaaaatggt
     1861 attgagctcc acggcattct gcttatacga atcgtaaaaa tccaggaagt cgaggatgct
     1921 gatgttccgt ttctcgtaat tttttatgac ctccccggtc agcctttcga aattggccgc
     1981 gaactttggg tcgatggagc ggaacaggcg ctcctgtgca aacgcttttt gcagcgatac
     2041 cgccacattc tcttccacgg tggcctgagt gaatttttgg gtggcggccg tgttatcgat
     2101 cagcgatttc gcgttcctga tgttgccctg gttgtggttg aacaccggca ggtcgataga
     2161 gacgccgatt ccataataac catagagaaa gctgccggcc tcgtcatagc cgagggagag
     2221 gctcggatcc ggtacggcca gcgctctttg ataatcgtag ttgagctggt tgatctttgt
     2281 attggtccgg gcaatctgca gatccgtcct gttgcggtag gcggaatcca gaagcgtatt
     2341 cagggaatac tgccccggat ccagtttgtc tatcgcgctg gaatctacga cgggatcgac
     2401 aaagacattg ggcctggcct ggagcacgag gcgcagttcg ctttcggtgc tgttaatgga
     2461 attgatcagg tcgctgtact ccgcctggaa gctgtaaagc tgcgcgcgga tccgcacgac
     2521 ttccttttcg gcgatataac c
//
LOCUS       DATGKE010000568         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1067673, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000568 DATGKE010000000
VERSION     DATGKE010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067673"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VKU83_09655"
     CDS             <1..1003
                     /locus_tag="VKU83_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLI93865.1"
                     /translation="EPEDPRSYYSIARRLAREIPNSFHANQYDNLANRLAHYETTGPE
                     IWQQTDGKITHLVVATGTGGTVTGTAMYLKERNAAIQVWAIDPFGSLLTKFFRTGEID
                     MNEVHPYVTEGIGEDFVPQNYDMRFIDVFEQVSDKDAALMARRIAREEALFCGYSAGT
                     AVQGLMQLKGLLKKDDLVVVILHDHGSRYVGKIYNDQWMMERGFMAVKTFKDIIASRG
                     ANRLITAAADQTVNEAFEMMQKYHIENLPVLQGGELIGAVSESGLFMRMMTGTTDLKA
                     KKIGDVLEKPYPVVGFDTPVERLSGLINRENGAVLGRDDSGNYHIVTKYDVIHALGA"
     gene            1465..2247
                     /locus_tag="VKU83_09660"
     CDS             1465..2247
                     /locus_tag="VKU83_09660"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HLI93866.1"
                     /translation="MPAFTDQIGRTILLSAAPVRVISLVPSQTELLYTLHADVAGITK
                     FCIRPDVWYRNKPRVGGTKNVDREKIAALCPDLIIANKEENDRDQVESLAGCYPLWVS
                     DIHTLSDALQMIRSVGELVDRPREASAIATRILDNFSGLIPGPPVTAAYLIWRTDSPL
                     SFMAAGGDTFIHDMLVRCGFVNPFAGHTRYPTVEPAALAACNLVLLSSEPYPFREKHQ
                     IELQALLPQAKVRLVDGQFFSWYGSRLLGAPRYFRDLLSGLP"
     gene            2302..>3239
                     /locus_tag="VKU83_09665"
     CDS             2302..>3239
                     /locus_tag="VKU83_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93867.1"
                     /translation="MQFIDPDSYSTGEQNKPSRKKPIFPVTDALRNYLKNHGREVKLP
                     VSYRDLQQYITYSVPLQDKNQQDTLWESVLYDMRHWDFIRGGLVQMYAILKTEGDLSY
                     TKHLDVARIDYCSFANSNPFRIRIVNKFNDNYDHYYIKQADASRIYGLELEHLLSPNR
                     ITYFTHLNTLVEEHIPGIPGDLFIRNFIGDPLTNKIRLSKEFVKFNERCFVRLLGDMR
                     SYNFVVDITPDIEDYQYRIRAIDFDQQSYEGRKNLYIPQFFKENNAFVNLCLQHLNKE
                     SIEQYQTEERTMMAFRVASSRFRLMELLNIMSHHRGL"
BASE COUNT          788 a          954 c          781 g          716 t
ORIGIN      
        1 ggaaccggaa gacccccggt cctattactc cattgcccgc cgcctcgcac gcgaaatccc
       61 gaattccttt catgccaacc agtatgacaa cctggctaac cgtctggcac attatgagac
      121 gaccggcccc gagatctggc aacagacgga cgggaaaatt acgcatctgg tcgtcgcaac
      181 aggcaccggc ggcaccgtta cgggcacggc catgtacctc aaggaaagaa atgccgctat
      241 ccaggtgtgg gcaatcgacc cgttcgggtc cctgctgacg aaattcttcc gaaccggaga
      301 aatcgatatg aacgaagttc acccttatgt cacggaaggc atcggcgaag attttgttcc
      361 gcaaaattat gacatgcggt tcatcgatgt ctttgaacag gtctccgaca aggacgccgc
      421 cctcatggcc cgccggatcg cccgggaaga ggcgctcttt tgcggctata gcgccggcac
      481 agccgtccag ggattaatgc agctcaaagg tctgcttaaa aaggacgacc tcgttgtcgt
      541 catcctccat gatcatggca gccgctacgt aggcaagatc tataacgacc agtggatgat
      601 ggaaaggggc ttcatggccg tcaaaacctt caaggatatc atcgccagcc gcggcgcaaa
      661 ccggctgatt accgccgcgg ctgaccagac cgtcaatgaa gcattcgaga tgatgcaaaa
      721 gtaccatatc gagaacctgc cggtattgca gggaggagaa ctgatcggag cggttagcga
      781 aagcgggctc ttcatgcgca tgatgaccgg cacgaccgac ctgaaggcaa agaagatcgg
      841 tgacgtgctg gaaaagccct atccggtcgt cgggtttgat acccccgttg aacgcctcag
      901 cggattgatc aaccgcgaga acggagctgt actgggccgg gacgacagcg gaaattatca
      961 tatagtaacc aaatatgatg tcatccacgc gctcggggct tgaacggcgt cacatcgtac
     1021 tattactatc gtcctatatg ggaatttccc cgtccttttc gcgtccccta ccatcctgcc
     1081 gggtaaaaga tgaagctgtc aaatagggca aaacctctat tggcaggcaa tttttaacaa
     1141 attatttttg agtcagaagt tgattgatag catcaatcac cgatatccga aaagccgaaa
     1201 cctcatcctg aatccaatat ccggaaaaag ccggattcaa tatccaaaga gattccattt
     1261 caagtccaat atcagtcaac ttctactttt tcggactacc gtcaagccct aatccataat
     1321 cctgaatagc tgaaaaacct gttgatcttc agaccctcct gctaaattcc gggcaacaaa
     1381 agtttagcag gaggccccta cccgtccacc gtgcccgccg ggtcctccgc cgcatttcct
     1441 accttgcagg gacaatcctt gtgcatgccc gcattcacag accagatcgg acgaaccatc
     1501 ctcctttcag ccgcccctgt ccgggtcatt tccctcgtcc cctctcaaac ggaactactg
     1561 tacaccctcc atgctgacgt cgcgggcatc accaaattct gcatccggcc tgatgtctgg
     1621 taccggaaca aaccgcgggt agggggaacg aaaaatgtcg acagggaaaa gattgccgcc
     1681 ctctgcccgg acctgatcat tgccaacaag gaggagaatg accgtgatca ggtcgagtct
     1741 ctcgccggat gctatccgct ttgggttagc gacatccata ctctctccga cgccctgcag
     1801 atgatccgca gtgtcggaga acttgtcgat cgtccacggg aggcatccgc cattgcgaca
     1861 aggatccttg acaatttctc cgggcttatc ccgggtccgc ccgtcaccgc tgcctacctc
     1921 atctggcgaa ccgactcccc gctgtcgttt atggcagccg gcggcgacac attcatccac
     1981 gacatgcttg tccgctgtgg cttcgtcaac ccgtttgcgg ggcatacccg gtatccgacc
     2041 gttgaacccg ccgcgctcgc tgcatgcaac ctggtgcttc tgtcttcgga gccctatcca
     2101 tttcgcgaaa aacatcaaat cgaacttcag gccctgctcc cgcaagcgaa ggtccgtttg
     2161 gttgatggcc aattcttcag ctggtacggc agccgcctcc tcggcgcccc ccggtatttc
     2221 cgcgatttgc tgtcggggct tccataggtc tccggtaaaa atgcgatctt tgaccccggt
     2281 tcatcaccaa acatccggag catgcagttc atcgaccccg attcctattc gactggagaa
     2341 cagaacaaac cgtcccggaa aaaaccgatt ttcccggtca ccgatgccct gcgaaattat
     2401 cttaagaacc atggccggga agtaaaactt ccggtctcct atcgggatct gcaacagtac
     2461 attacctatt cggtcccgtt gcaggacaag aatcagcagg acaccctttg ggaatccgtg
     2521 ctctacgata tgcggcactg ggactttatc cgcggcgggc tcgtgcaaat gtatgccatc
     2581 ctgaaaacgg agggtgatct gagttatacg aaacacctgg atgtcgcgcg gattgactat
     2641 tgcagcttcg ccaactccaa ccccttccgc atccggatcg tcaacaaatt caatgacaac
     2701 tacgatcact actatatcaa acaagccgat gcgtcccgca tctacgggct ggaactggag
     2761 catttgctgt cgccgaatcg catcacctac ttcacccatt tgaatacgct cgttgaagaa
     2821 catatccccg ggattcccgg agacctgttc atccggaatt ttatcggcga cccgctcacc
     2881 aacaagatcc ggctgtccaa agaattcgtg aagttcaatg agcgctgctt cgtgcgcctg
     2941 ctcggagata tgcggtcata taattttgtc gtcgacatca cgccggatat tgaagactac
     3001 caataccgga tcagggctat cgatttcgat cagcagtcct acgaaggccg gaagaatctt
     3061 tatatccctc agttctttaa ggaaaacaac gcctttgtaa acctgtgcct gcagcatctg
     3121 aacaaggagt ccattgaaca ataccagacc gaggaacgaa cgatgatggc tttccgggtg
     3181 gcttcctccc ggttccggct tatggaactg ctgaatatca tgtcccatca ccgcggact
//
LOCUS       DATGKE010000569         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_667202, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000569 DATGKE010000000
VERSION     DATGKE010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667202"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VKU83_09670"
     CDS             complement(<1..641)
                     /locus_tag="VKU83_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HLI93868.1"
                     /translation="MPKVSLTIWIMIAMLSGVLLGLWVHYGGSAVQIDGFNHMATVIT
                     DDIFLRLIKMIIAPLVFSTLVVGIARLGDIKAVGRIGGKTLLWFLCASFLSLLLGLLI
                     MKSMSLGTELNLPLPSAAAGTVLDQVPMSLKSFITHIFPQSIFEAMARNEILQIVIFS
                     LFVGTAVAALDDKAPLLLAFCEHAAAVMLKITGYVMVMAPLVVFAALASTVATS"
     gene            761..2257
                     /locus_tag="VKU83_09675"
     CDS             761..2257
                     /locus_tag="VKU83_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLI93869.1"
                     /translation="MSLFRKKTLATLLAQASESEKGLKRSLGVWSLIALGIGAIIGAG
                     LFVRTAAAAGEHAGPAVTISFIIAAVGCCFAGLCYAEFASIIPIAGSAYTYAYATMGE
                     LIAWIIGWALILEYALGAATVAISWSEYLNNLLYSLFQYKFPYEWCHSPMQSFLDTAG
                     TMHHGIINIPALFILVILTLLLIKGMQESAVVNGIIVFIKVAIVIVFIFIGWHFIKGI
                     NHTPYIIPADAKPAIEPDGTTYSYLPFLNHGWGGVLRGAAVVFFAFIGFDAVSTAAQE
                     AKNPKKDMPMGILGSLAICTILYILFSFVLTGVAPYTDFIKAGKEASVSYAIQTYMPG
                     YNWLSVLVTIAILLGFSSVILVMLMGQSRVFYTMSTDGLLPKVFSDIHPKFRTPYKSN
                     IILFVFVGLFAGFLPESVAGDLTSIGTLLAFVLVCAGVIILRKRDPGLHRPFKTPLVP
                     LVPILGMIVCAAMIFSLWGTTLLSAFIWMIIGLCIYFGFSRNSSKLKE"
     gene            2351..>3039
                     /locus_tag="VKU83_09680"
     CDS             2351..>3039
                     /locus_tag="VKU83_09680"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLI93870.1"
                     /translation="MGRIFEVRKATMFARWDRMAKQFTRVGKEIVIAVKAGGPDPATN
                     PALRRCFQNARSVGMPKDRVEAAIKRAQGKEMTNYEEILYEGYAPHGVALLVETATDN
                     HVRTVANIKSHFTKGHGSLGNSGSVSFQFKKMGVFKLKPEGLNADDLELELIDFGLEE
                     MGEGTGEHDEPVIVLRVAFNDFGNMQKALEEKHIAPISSGIEWIPLTTVELPEDQAQD
                     VLKLVDRLEQD"
BASE COUNT          681 a          877 c          799 g          682 t
ORIGIN      
        1 gatgtggcca ccgtcgaggc cagcgccgca aacaccacca gcggcgccat caccatcaca
       61 tagccggtga tcttcagcat caccgcggcc gcatgctcgc agaaggcgag cagcaggggc
      121 gccttatcgt ccagcgccgc cacggcggtg ccgacgaaca gcgagaagat cacgatctgc
      181 aggatctcgt tgcgggccat cgcctcaaat atgctctgcg gaaaaatgtg cgtgataaac
      241 gatttcagcg acatcggcac ctggtcgagg acggtgccgg ccgctgccga aggcagcggc
      301 agattcagct cggtccccag ggacatcgac ttcatgatca gcagtccgag caggagggac
      361 aaaaaggacg cgcacaggaa ccacagtaaa gtcttgccgc cgatgcggcc taccgccttg
      421 atatctccca gcctggcgat cccaaccaca agcgtcgaaa agaccagcgg cgcgatgatc
      481 attttgatta accgcaggaa aatgtcatcc gttatgacgg tcgccatgtg attgaaccca
      541 tcgatctgaa cggcgctccc cccgtaatga acccacaaac ccagaagcac gcccgacaac
      601 atggcaatca tgatccatat ggtaaggctg accttgggca tagatgcggc aataatcctg
      661 caaacaatcg aattttattg atttggcaaa actttaatgc caaaaatgcc tattttcggc
      721 ccaaggctaa agtccacatt tttctcaaaa cgaaccatat atgagtttat tccgaaaaaa
      781 gacactggcg accctgcttg cgcaggcctc cgaatctgaa aagggcttga aacggtcgct
      841 cggtgtctgg agcctgattg cgctgggcat cggcgccatc atcggcgccg gtctctttgt
      901 ccgtacggcc gcggcagcgg gtgaacatgc gggacccgcc gttaccatct cgttcattat
      961 cgcggcggtc ggttgttgct tcgccggact ttgctatgcg gaatttgcct cgatcatccc
     1021 cattgccggt agcgcctata cttacgctta cgccacgatg ggcgagctga tcgcctggat
     1081 catcggctgg gcgctcatcc tcgagtatgc cctcggcgcc gcgacggtcg ccatctcatg
     1141 gagcgaatac ctgaacaacc tgctttattc tctctttcaa tataagttcc cttatgaatg
     1201 gtgccactcg ccaatgcagt cctttctcga caccgcgggt acgatgcacc acggtattat
     1261 caatatcccc gcccttttca ttctcgttat cctgacgctg ttactgataa agggaatgca
     1321 ggaatccgca gtcgttaatg gcatcatcgt gttcatcaag gtggcgatcg tgattgtctt
     1381 catcttcatt ggctggcatt tcattaaagg aataaaccat acgccctata ttattccggc
     1441 cgacgccaaa cctgcgatcg aacccgacgg taccacctac tcctatctgc ccttcctcaa
     1501 tcatggctgg ggtggcgtcc tgcgtggcgc cgcagtcgtt ttctttgcct tcatcggctt
     1561 cgatgccgtg tcaaccgccg cccaggaagc caaaaatccg aaaaaggata tgcccatggg
     1621 catcctcggt tccctcgcaa tttgtacgat actctatatt ttgttctcgt tcgttctcac
     1681 cggtgtggcg ccatataccg acttcatcaa ggccggcaag gaagcctccg tatcctacgc
     1741 catccagaca tacatgcccg gctacaactg gttgtccgtc ctggtgacga ttgccatcct
     1801 gctggggttc agttcggtga tcctggtgat gctgatgggc cagagccggg ttttctacac
     1861 catgtcgacc gacggtttgc ttcctaaggt attctcggac attcatccaa agttccggac
     1921 gccttacaag agcaatatca tcctcttcgt atttgttggc ctgttcgcag ggtttcttcc
     1981 cgaaagcgtt gcgggcgatc tgacatccat cggaaccctg ctggcgtttg tgctggtatg
     2041 tgccggggtc atcatcctgc ggaaaaggga cccgggcctg caccgtccgt tcaagacacc
     2101 gctcgtacct ctcgtaccga tcctgggcat gatcgtctgc gccgcgatga tcttcagcct
     2161 gtgggggacc accctgctca gcgcatttat ctggatgatc atcggcctct gcatttattt
     2221 cggctttagc aggaacagca gcaaactgaa ggaatagccg ccgtgaatgc ctgcaactta
     2281 taggccgccc cgaatttttg gggcggtttt ctttgtttag taaatttgcc cccctataaa
     2341 gatataatct atgggtcgga tatttgaagt caggaaggct accatgtttg cccgctggga
     2401 ccggatggcc aagcaattca cccgcgtcgg gaaagaaatc gtcatcgctg tcaaggccgg
     2461 gggcccggac cctgccacga atccggcttt gcgccgttgt tttcagaatg cgaggagcgt
     2521 gggcatgccc aaggaccggg tcgaagcggc catcaaacgc gcccagggaa aggaaatgac
     2581 caattatgaa gagatcctct atgagggcta tgcgccgcac ggggtcgcgc tcctcgtaga
     2641 gaccgccacc gataatcacg tccgcaccgt cgccaacatc aaatcccatt tcaccaaggg
     2701 ccacgggagc ctcggcaaca gcggcagcgt cagctttcag ttcaagaaaa tgggtgtctt
     2761 caagcttaaa ccggaggggc tcaatgcaga tgatctcgag ctcgagctga tcgatttcgg
     2821 gctggaggag atgggcgagg gaaccggcga acacgacgaa ccggttattg tccttcgcgt
     2881 tgcatttaac gactttggaa acatgcaaaa ggcgctggaa gaaaagcata ttgccccgat
     2941 cagctccgga atcgagtgga tccccctcac taccgtcgaa ctgccggaag atcaggcaca
     3001 ggatgtgctg aagctggtcg accggctgga acaggacga
//
LOCUS       DATGKE010000570         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_665855, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000570 DATGKE010000000
VERSION     DATGKE010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665855"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /gene="alaS"
                     /locus_tag="VKU83_09685"
     CDS             <1..1966
                     /gene="alaS"
                     /locus_tag="VKU83_09685"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLI93871.1"
                     /translation="QPLPARHVDTGMGFERLVRVLQGKHSNYDTDVFTGTIAGIGNIV
                     GRSYKGDDTKASIAFRVIADHIRAIGFTIADGQLPANTDAGYVIRRILRRAVRYYYSY
                     LEYRQPLLYQLMPLLAAQFKDVFPELQQQVGFVSKVVREEEEAFLRTLEKGLKRIEEV
                     MKVSAAGGVMPGAAAFELYDTYGFPEDLTRLIAAENNLTVDGAGFQLEMQRQKDRSRA
                     ATAIDATDWVVLIDNPLTEFVGYDLLETRTRIAKYRKITAKGKESWQIVLEATPFYAE
                     SGGQEGDTGILVSGGETIPVLDTKKENDLIIHFTGVLPAGLNGPVIARVDAAKRKRTE
                     VHHSATHLLHAALRAVLGSHVAQKGSLVNDEQLRFDFSHFARMTEGEIAAVEEMVNAK
                     IRENIPVVTREMPKAEALRLGAMALFGEKYGDTVRVVIIDPTYSIELCGGTHVGATGE
                     LGLFKIWHESAVAAGVRRIEAVSGKAAEEWVGEQLAVARSAREVLKNPADLPKAIENL
                     VAENGDLRKKLEKAEARQLADLRGRLLDKVKAIGGVSFIGEIVDVPAADALKRLSFEL
                     KPALSALSGAPPYLVVLAANIEGKAAVSVMLDERLAAGRGLQAPSIIKEQVAPLIRGG
                     GGGQKTLATAGGQEAGNLSQVIEKVEALLK"
BASE COUNT          471 a          626 c          704 g          404 t
ORIGIN      
        1 gcagccgctg ccggcccgcc acgttgacac cggcatgggt ttcgagcggc tcgtaagagt
       61 gctgcagggg aagcattcca actatgacac ggatgtcttc acgggcacga ttgcggggat
      121 cgggaacatc gtggggcggt cctataaggg cgacgacacg aaagcttcga tcgctttccg
      181 tgtgatcgcc gatcacatcc gggccatcgg ctttacgatc gccgacggtc agctccccgc
      241 caatacggac gcgggctacg tgatccggcg gatcctgcgc cgggcggttc gctattacta
      301 ctcctacctc gaatacaggc agccgctgct ctaccagctt atgcctttgc tggccgcgca
      361 gttcaaggat gtgttcccgg aattgcagca gcaggtcggg tttgtctcca aggtcgtcag
      421 ggaagaggag gaagcgtttt tgcggacgtt ggaaaaagga ttgaaacgga tcgaggaggt
      481 tatgaaggta tcggcagccg gcggggtgat gccgggggcg gcagccttcg agctgtacga
      541 cacctatggg tttcccgaag acctgacccg gctgatcgcc gcggaaaaca atttaaccgt
      601 agacggagca gggttccagc ttgagatgca gcggcagaaa gaccggtcgc gggcggcgac
      661 ggcgatcgac gcgacggact gggtcgtgct catcgacaac ccgctgacgg aattcgtggg
      721 ctatgacctc cttgaaaccc ggacgcgcat cgccaaatac cggaagataa cggcaaaagg
      781 caaagagtcg tggcagattg tgctcgaggc cacgcctttc tatgccgaaa gcggcggtca
      841 ggagggagat acgggcatcc tggtttcggg tggggaaacc atccctgtcc tggataccaa
      901 aaaagagaat gacctcatca tccatttcac cggcgtcctg ccggcggggc tcaacgggcc
      961 ggtgattgcc agggtcgacg cagcgaaacg aaagcgaacc gaagtgcatc actccgcgac
     1021 ccacctgctg catgccgcct tgcgtgccgt tctgggtagc catgtcgccc agaagggatc
     1081 gctggttaac gacgagcagc ttcgctttga tttttctcat tttgcccgga tgaccgaagg
     1141 cgagatcgcg gcggtggagg agatggtgaa cgcgaagatc cgggaaaata tcccggtcgt
     1201 cacccgcgag atgccaaagg cggaagctct ccggctgggc gcgatggcac ttttcggcga
     1261 aaaatatggc gataccgtcc gggtcgtcat catcgacccc acctattcca tcgaactatg
     1321 cgggggcacc cacgtcggcg ctaccggcga gctgggcctg ttcaagatct ggcatgagtc
     1381 tgctgtcgcc gcaggcgtgc gccgcatcga agcggtcagc ggaaaggctg cggaagaatg
     1441 ggttggcgaa caactcgcgg tcgcccgctc cgcgagggaa gtgttgaaga acccggcgga
     1501 tctgccgaaa gcgatcgaaa acctcgtcgc cgagaacggc gacctcagga agaagctcga
     1561 aaaggctgag gcccggcagc tggcggacct ccgcggccgc ctgctggaca aggtaaaggc
     1621 catcggcggc gtgtcgttca tcggggagat tgtcgacgtc ccggcggccg atgcgctgaa
     1681 gcgactgagt tttgagctga agccggcgct ctcggccctg tcgggcgccc caccatacct
     1741 ggtggtgctc gcggcgaata tcgaagggaa ggcggcggtg tccgtcatgc tggatgagcg
     1801 gctggcagcc ggccggggcc ttcaggctcc cagcatcatc aaggagcagg tcgctcccct
     1861 gatcaggggt ggcggcggcg gtcagaagac cctggcgacc gcgggcggcc aggaggccgg
     1921 aaatctttcc caggtgatcg aaaaggttga agcgctcctg aaatgaaaac cccccgattt
     1981 aacatttccg aaagttttcg tatttccgga aaaccctgta tatttgatct acgaaaacca
     2041 acgtagatca aaaacctttt gtttatcaaa aatcaccact gatgaaaaga tacctcccca
     2101 agatcgccgg cctcgccatt ctggtcctct tgatccggac ttccggcttt gcccaggatg
     2161 acagcacgcg ggacaacaat gacgatcgcg cgcagcgccg tgtcg
//
LOCUS       DATGKE010000571         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_860767, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000571 DATGKE010000000
VERSION     DATGKE010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860767"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VKU83_09690"
     CDS             <1..686
                     /locus_tag="VKU83_09690"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLI93872.1"
                     /translation="RYFPVAHVMVIKEIIQFITSGTRVFYITGNHDEMMRRYTDLQMG
                     NFTLTDKLVLEIDNKMTWIFHGDVFDNTTKGGAKVLAKLGSSGYNLLIRFNRFINFIL
                     KALGRERVSISKRVMAGVNAAVSKINDFELIAAELAIEKRYDYVICGHIHQPQKKRVE
                     TKNGQVTYLNSGDWVEHCTALEYRNNEWKIFEYDEKEFPATPAIEMKPSVSVLTDEIS
                     FYINSLAAI"
     gene            698..1735
                     /locus_tag="VKU83_09695"
     CDS             698..1735
                     /locus_tag="VKU83_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLI93873.1"
                     /translation="MRIFYAVQATGNGHISRAMELLPHLERYGRVDVFLSGANNSLVP
                     DIPVRYRSKGLSLFYTNTGGLNYVEILRQLSPPRVWREVRDLPVEKYDLVINDFECIT
                     SLACAYKKVASVNFGHQASFVSNKVPRPEKSDPVGAWILRNYARATQYVGLHFLQYDD
                     FILPPVIKKEILQADACDMGHITVYLSSYGDATVRQYLRPLKEWRWQVFSKEVSRPVE
                     DGNILFLPVGKTAFNRSLITCSALLTGAGFETPAEALYLGKKLMVMPIRGQYEQFCNA
                     AALAKMGVPVLHALDGEFERTFRAWMEQRKRPRLHLDHSTDAIVSYLMHHCAPRKKGA
                     LDLLYPDAVFN"
     gene            1791..>2432
                     /locus_tag="VKU83_09700"
     CDS             1791..>2432
                     /locus_tag="VKU83_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI93874.1"
                     /translation="MKLLVIEDEPSLLASITRYFRKEQFLCDEATTFGEALQKIEDFQ
                     YDCIILDINLPGGSGLQLLKLLRSDKKSDGVIIISAKDSLDDKITGLDNGADDYLTKP
                     FHLPELSARVKSLFRRKYSAGNPSIDVNGLHIQLGSRIVVSAGQALALTKMEYDLLLF
                     LATNKNRVVSRQAIAENLFDGPSDQAPGPEFVYSHIKNLKRKLKEGGCNEMIQT"
BASE COUNT          588 a          685 c          655 g          504 t
ORIGIN      
        1 aacgctattt cccggtagcg cacgtcatgg tcatcaagga gatcattcaa tttatcacca
       61 gcggaacccg ggtcttttac ataaccggca accacgacga gatgatgcgc cggtacaccg
      121 acctgcagat gggtaatttc acgctcaccg acaagctggt gctggagata gacaataaga
      181 tgacctggat ctttcacggc gacgttttcg acaacaccac gaaaggcggg gcaaaggtcc
      241 tcgccaagct gggaagcagc gggtataacc tgcttatccg gttcaaccgc ttcatcaatt
      301 ttatcctgaa ggcgctcggc cgggagcggg tttcgatctc caaacgggta atggccggcg
      361 tcaatgcagc cgtcagcaag atcaacgact tcgagctgat cgccgcagaa ctcgccatcg
      421 aaaagcgata tgactatgtg atctgcgggc atatccacca gccgcaaaag aaacgggtcg
      481 aaacaaagaa cggccaggtc acctacctga attcgggcga ctgggtcgag cactgcacgg
      541 cgctggaata ccgtaacaac gagtggaaga tcttcgaata tgatgaaaag gaattcccgg
      601 ccactccggc tattgagatg aaaccaagcg tcagcgtcct gaccgatgag atcagcttct
      661 atattaattc actggcagcc atctaaatcc ccgcaccatg agaatatttt acgcagtaca
      721 ggctaccggg aacggtcata tcagcagggc catggagctc ctcccgcatc ttgagcgcta
      781 tggcagggtg gacgtcttcc tgagcggcgc caacaacagc ctcgtgccgg atatcccggt
      841 ccgctaccgg agcaagggtc tcagcctgtt ctatacgaat accgggggcc tgaattatgt
      901 ggagatcctc aggcagctgt cgccgccgcg cgtatggcgg gaggtacggg atctgccggt
      961 cgagaaatac gatctcgtga tcaatgactt cgagtgtatc acttcccttg cctgcgccta
     1021 taaaaaggtg gcatccgtga acttcggcca ccaggccagc ttcgtttcca ataaggtccc
     1081 ccgtcctgaa aagagtgatc cggttggcgc ctggatcctg cggaactatg cccgggccac
     1141 gcagtatgtc ggcctccatt ttctgcaata tgacgatttc atccttcccc cggtgatcaa
     1201 aaaagagatc ctgcaggccg acgcctgcga catgggtcat attaccgtct acctttcttc
     1261 ctatggcgac gcgacggtca ggcaatacct ccggccactc aaggaatggc gctggcaggt
     1321 tttctccaag gaggtcagcc ggccggtaga ggacggaaat atcctcttcc tgcctgtcgg
     1381 caagactgca ttcaaccgca gcctgatcac ctgtagcgcg ctcctcacgg gcgccggctt
     1441 cgagacgccc gccgaggcgc tgtatctcgg caagaagctg atggtgatgc ccatcagagg
     1501 ccagtacgaa cagttctgca atgcggcggc cctcgcaaaa atgggggtgc ccgtgctgca
     1561 tgcgctcgac ggggaatttg aaaggacgtt ccgcgcctgg atggagcagc gtaagcggcc
     1621 gcgtttgcac ctcgatcaca gcacggacgc catcgtcagc tatctcatgc atcattgcgc
     1681 accacggaag aagggtgcgc tggacctgtt gtatcccgat gcggtgttca attaggcgca
     1741 ccgcgaaaat tcttattcgt tcaggggcat ttttgcagta aattaccata atgaagctcc
     1801 tggtaatcga agacgaaccg agtctcctgg cgagtataac ccgctatttc cgtaaggaac
     1861 aatttttatg cgatgaggca acgacattcg gcgaggcgtt gcagaagatc gaagatttcc
     1921 aatacgattg tattatcctg gacatcaacc tgccgggtgg aagcgggcta caactgctca
     1981 aactgctccg gagcgacaag aaaagcgatg gcgtcatcat catttctgcc aaggactccc
     2041 ttgatgataa gataaccggc ctcgacaacg gcgccgacga ctacctcacc aaaccctttc
     2101 acctgccgga gctgagcgcg cgcgtaaaat cactgtttcg ccgaaaatac tcggctggga
     2161 atccctcgat agacgtcaac gggctgcata tccagctcgg ttcgcggatc gtcgtctccg
     2221 ccgggcaagc tctggcgctg acgaagatgg aatacgacct gctccttttt ctcgccacga
     2281 acaagaacag ggtagtcagc cgccaggcca tagcggaaaa tctttttgac ggcccatccg
     2341 accaggcgcc tggacctgag ttcgtctatt cccatatcaa aaacctgaag cggaagctga
     2401 aggaaggtgg ctgcaatgaa atgattcaga cg
//
LOCUS       DATGKE010000572         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_335383, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000572 DATGKE010000000
VERSION     DATGKE010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335383"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VKU83_09705"
     CDS             complement(<1..515)
                     /locus_tag="VKU83_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HLI93875.1"
                     /translation="MKIFQVDAFAGELFHGNPAAVVPLESWLPDGLMQQIAMENNLAE
                     TAFFVPHGNAFLLRWFTPTVEVALCGHATLATTHVLFEHLGYRTNPVVFRTVASGDLR
                     AKRESPNRYTLDFPQTPALPVDPGPLGVLFESLGLPPVPVLKGPFDYLVVLDSENAIA
                     DLRPDFRRLATV"
     gene            596..1276
                     /locus_tag="VKU83_09710"
     CDS             596..1276
                     /locus_tag="VKU83_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI93876.1"
                     /translation="MKILIVEDELDLAQSMATYLKGENYLCELAADRKSALEKIEVFD
                     YDCILLDISLSDGNGLSVLKELKANNKTDGVIIISARNSIDDKIDGLNMGADDYLSKP
                     FHLPELNARIAAVIRRRRFEGNRTLVLNELVIDLIGKTVTVGTEPVDLTRKEYDLLLY
                     LASNKNRVISKNAIAEHLSGEQAMSYDNLDFIYSHMKNLKRKLHQAGCSDYIRSLYGM
                     GYKFEIKT"
     gene            complement(1285..1737)
                     /gene="dtd"
                     /locus_tag="VKU83_09715"
     CDS             complement(1285..1737)
                     /gene="dtd"
                     /locus_tag="VKU83_09715"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HLI93877.1"
                     /translation="MRVVIQRVKHASVTIGDRVRAAIKEGLLVLVGIEDADTAEDAAW
                     LTNKIINLRVFNDEAGVMNISVKDMGGDILLVSQFTLHASTRKGNRPSYIRASKPETA
                     IPLYEGFIRLLETELQKEIFTGEFGADMKVELVNDGPVTIWIDSKNRE"
     gene            complement(1773..>3362)
                     /locus_tag="VKU83_09720"
     CDS             complement(1773..>3362)
                     /locus_tag="VKU83_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93878.1"
                     /translation="SRARRVLNSMPADLAAEIGKEGPRYYWGGVGNFFAGPPYVGAIV
                     ILLAVVGFFVLDDRHKWWILGVAALSIAMSWGGYFYGFNHFLLKWLPLYSKFRAPSMI
                     LVIPTFLFCMMATMTLDRILRSENRARLWQQYKWGLLALGGIFVFVGWLYFGFRYSSE
                     WETDLLKIAAARGSSAIGYMTDFVQALRADRQSIFGESLLRSLTLTIVAALILAFYIR
                     KRMRPSLLLATIGLLVFADVMTIDLRYLNDSNYMEPDDYEENFTATNADKKIDQDKGY
                     YRVFDLRDSVSNALAYGAMTAYFHFSIGGYHAAKLSIYEDLINRQLANFPNCRPVINM
                     LNTKYIIKPEKPGKDSVLLNKNSLGPVWFVRGVRFEQTPNEVMNDLTHFDPKDTAIVF
                     SADRSRVKWGTTGDSLGKISLVRNDNDEITYLSQAPGERFAVFSEVYYNRGWRAWIDE
                     HEAPIIRTNYVLRGLSVPPGRHVIRFFFRPLSYYLGRQMQWMANILFLLLVAGTVIVT
                     IQESRRLPRPVSDDALLQKAA"
BASE COUNT          785 a          922 c          946 g          709 t
ORIGIN      
        1 accgtcgcca accgcctgaa gtccggccgc aggtccgcga tggcattctc ggaatccagc
       61 acgacgagat agtcgaacgg gcccttgaga acgggaacgg ggggcaggcc cagggattcg
      121 aacaaaacgc ccagcggacc cggatccacc ggcaaggcgg gggtttgcgg aaaatcaagg
      181 gtatagcggt ttgggctttc ccttttcgcc cggagatcgc cggatgcaac cgtgcgaaaa
      241 accaccgggt ttgtccgata gccgaggtgc tcgaacagca catgcgtggt cgccagcgtg
      301 gcgtggccgc atagcgcgac ttccacggtt ggagtaaacc accgcaacag aaatgcattg
      361 ccatgcggga caaaaaatgc cgtctccgcc aaattgtttt ccattgctat ctgctgcatt
      421 aagccgtccg gcagccacga ttccaacgga acgacagccg ccgggttacc atggaataat
      481 tcacccgcaa acgcatcgac ctgaaagatt ttcatatgaa tgttatttaa cctgctttat
      541 tttcacgcct gtatattaca gaattcctac agaatataag catactttta tccagatgaa
      601 aatcttgatc gtcgaagacg aactggacct ggcgcaaagc atggctacct acctgaaagg
      661 agagaactac ctgtgtgaac tcgctgctga ccgcaaaagc gcccttgaaa agatagaagt
      721 ttttgactac gattgcatcc ttcttgacat ttcgctttcc gacggcaacg ggctttccgt
      781 ccttaaggaa ttaaaggcga ataacaagac ggatggcgtc atcattattt ccgccaggaa
      841 ttcgatcgac gacaagatag atggcctcaa catgggcgcg gatgattatc tcagcaagcc
      901 cttccacctt ccggaactca acgcgcggat cgcggcggtc atccgcaggc ggcgttttga
      961 gggcaatcgc accctcgtgc tgaatgaatt ggtcatcgac ctgatcggca agacggttac
     1021 agtcggcacg gagccagtgg atctcacgcg aaaagaatac gatttgctgc tttacctcgc
     1081 ttcgaacaag aaccgggtga tctccaagaa cgcgattgcc gagcatctgt ccggggagca
     1141 ggcgatgagc tatgacaacc tcgatttcat ttactctcac atgaagaacc tgaagcggaa
     1201 gctgcaccag gccgggtgca gtgattatat ccggtcgcta tacggcatgg gctacaagtt
     1261 tgagatcaag acctgacgcc ggcctcattc gcggttcttg ctgtcgatcc agatggtcac
     1321 cggtccgtcg ttgacgagct cgaccttcat gtcggctcca aattctcccg tgaagatctc
     1381 cttctgcaat tcagtttcca gcaagcggat gaagccctca taaagcggga tagcggtttc
     1441 cggtttgctg gcgcggatat aggacggccg gtttcctttc cgggtggaag cgtgcagcgt
     1501 gaactggctg accagcagga tatcgccgcc catgtccttc acggaaatgt tcatgacacc
     1561 ggcttcatcg ttgaagacgc gcaggttgat tattttattc gtcagccagg cggcgtcctc
     1621 cgcggtatcg gcgtcttcaa tgcccaccag gaccagcagg ccctctttaa tagctgcccg
     1681 gaccctgtcc ccgatagtaa ccgaagcgtg cttgacccgc tggatgacaa cccgcatttt
     1741 tttcggtaaa tataaggcgg gaatcgcggg ccttaagccg ctttctgcag caaggcatcg
     1801 tcgctgacgg gcctcgggag ccgccttgac tcctggatgg taacgatcac cgtgccggca
     1861 accagcaaca ggaagagaat gtttgccatc cattgcatct ggcggcccag gtagtaggac
     1921 agtggccgga aaaagaagcg gatgacatgg cggcccgggg gaacggacag cccgcgcagc
     1981 acgtagttgg tacggatgat cggcgcctca tgctcgtcga tccaggctcg ccagccccga
     2041 ttgtagtata cctcgctaaa gaccgcaaaa cgttcgcccg gtgcctggga gaggtaggtg
     2101 atctcgtcgt tgtcgttcct gacaagggat attttgccga gcgagtcgcc cgtggttccc
     2161 catttcacgc ggctcctgtc ggcgctgaag acgatggcgg tatccttcgg gtcaaaatgc
     2221 gtcagatcgt tcatgacctc gttgggggtt tgctcgaacc gcacgccccg gacaaaccat
     2281 accgggccca gcgaattctt gttcaggaga accgaatcct tgccgggttt ctcgggctta
     2341 atgatatact tggtgttgag catattgatc accgggcggc agttggggaa attggcgagt
     2401 tgccggttga tgagatcttc gtaaatgctc agtttcgcag cgtggtaccc accgatggag
     2461 aaatggaaat aggccgtcat ggccccgtaa gcgagggcgt tggaaacact gtcacggagg
     2521 tcgaagaccc ggtaataccc tttgtcctga tcgatcttct tatcagcatt ggtcgccgta
     2581 aaattctctt catagtcatc cggctccata taattggaat cattcaggta gcggaggtcg
     2641 atggtcatta catcggcgaa gaccagcagg cctatcgtcg ccaacaggag ggaaggccgc
     2701 attcttttgc ggatgtagaa ggcgaggatc agggctgcca caatggtcag ggtcagggac
     2761 cgcagcaggc tttcaccgaa gatcgattgc cggtccgcgc ggagtgcctg cacgaagtct
     2821 gtcatatacc cgatcgccga gctgccgcgc gcggcggcga tcttaaggag atcggtttcc
     2881 cactcgctcg aatagcggaa gccgaaatac agccagccca cgaatacaaa gataccgccg
     2941 agggccagga gtccccattt atattgctgc cagagccggg cgcggttttc ggaccgcaag
     3001 atcctgtcga gggtcatcgt cgccatcatg cagaacagga aggtagggat aaccaggatc
     3061 atgcttggcg cgcggaattt gctgtacagg ggaagccatt taagcaggaa atgattgaat
     3121 ccgtaaaaat aaccgcccca actcatggcg atggagagtg cggcgacgcc aaggatccac
     3181 catttgtgcc ggtcgtccag gacaaagaag cccaccacgg caagcaggat cacgatcgcg
     3241 ccgacataag gcggacctgc aaagaagttg ccgactcccc cccagtaata acggggacct
     3301 tccttaccta tctctgcggc gagatcagcc ggcatgctgt tcagcacccg ccgggcacgc
     3361 ga
//
LOCUS       DATGKE010000573         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1482367, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000573 DATGKE010000000
VERSION     DATGKE010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482367"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VKU83_09725"
     CDS             <1..588
                     /locus_tag="VKU83_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93879.1"
                     /translation="FVIFIATVGRPITGCMKNPLLPKIDSTDTTLHRNQYDSAAFYVS
                     ILINIYNTSGVFNDTFSDDASMKIYVVNGVVTIPGDSILNFPPIVFPQSGQDGIYKAT
                     WIADQIGVCNITGAYGIAAPGDSAVSIILQQTGTVTPKWQLVALADGTTTYAGGESVP
                     GWPPGFTFDPRKQSQDAFKLEQPGSYWNVWVYKDY"
     gene            610..1215
                     /locus_tag="VKU83_09730"
     CDS             610..1215
                     /locus_tag="VKU83_09730"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HLI93880.1"
                     /translation="MPLLVLVRHGQSVWNLQNRFTGETDVDLTDKGRMEACAAGDKLK
                     GLRFAHAFTSVLVRAIETLELILRTSGQPDCPVTRDRALNERNYGRLQGLNKAEVAKE
                     YGAEQVAEWRRSFSVRPPGGESLADTAARVLPFYHTSIEPMLKDAKDILVVAHGNSLR
                     ALVMWLEKISETAITGLDLPTGIPRRYELDASLRIVSVGYL"
     gene            complement(1230..2825)
                     /gene="bshC"
                     /locus_tag="VKU83_09735"
     CDS             complement(1230..2825)
                     /gene="bshC"
                     /locus_tag="VKU83_09735"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HLI93881.1"
                     /translation="MEWIAHHLPFRQTGSFSRIITDYLDQAESLRPFYVHAPDREGLD
                     AALEGRRKQPVDRAALVEALQEQYAAVKPVSRVSENIRLLEQDNVFTVCTAHQPNIFT
                     GHLYFIYKILHTIRLAGELAARHPDCRFVPVFWMGSEDADLEELGQIHLGGEELVWDT
                     RQTGAVGRMTTQGLDKLLFRIDGQLSVQPYGPQLVATLKEAYLKSPDIQTATFRLIHE
                     WFGAFGLIVLIADRPALKKLMVPVFGDDLFRQQPAAIVNETIRRITEQYKVQANPRDI
                     NLFYLKGDLRGRIDREGDAFVVHGSSLRFTEDQMKAELHQHPDRFSPNVILRGLFQET
                     ILPNLAFIGGGGETAYWLELRSLFEHYRVPFPVLLLRNSFLLIQRKWKEKMDRAGFSI
                     ADIFRPAESLVNELVRRESENALSLEREITEANRYYESLKALARPVDPSLEQHVEALQ
                     ARAVEPIRTLEKKLLRAEKRKFGDFRRQVQTLKDALFPAGQLQERIENILPWYAAYGP
                     DFIGSLYAQSPALAESFLILEMK"
     gene            complement(2959..3963)
                     /locus_tag="VKU83_09740"
     CDS             complement(2959..3963)
                     /locus_tag="VKU83_09740"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF010578.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HLI93882.1"
                     /translation="MPKKTFFCIDAHTCGNPVRLVAGGGPVLEGSNMSQKRQHFLKEY
                     DWIRKGLMFEPRGHDMMSGSILYPPHDPANDVAVLFIETSGCLPMCGHGTIGTITIAI
                     EEGLIQPKTPGVVRMEAPAGLVQIEYLQAGNKVRSVKLTNVPAYLAAEGVPVECPDLG
                     RLHVDVAYGGNFYAIVDPQENFSKLDDYSASQLISWSRVMRRRINETHPFVHPDDPTI
                     HGCTHILWAGQPIHPDATARNAVFYGDKAIDRSPCGTGTSARMAQWFAKGRLRVGDPF
                     IHESIIGSRFTGRVEQATTVGGRPAIVPSIEGWAKIYGFNTITIDPDDDPYAYGFQVL
                     "
     gene            complement(3969..>4986)
                     /locus_tag="VKU83_09745"
     CDS             complement(3969..>4986)
                     /locus_tag="VKU83_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLI93883.1"
                     /translation="ETSEIVAQAIRKAVEGAGLPAGVFGHVHGAGNETGRALVTHRHT
                     RAVGFTGSFGGGKALFDWAGQRKDPIPVFAEMGSINPVFLLPGKLRSGPKEVAALLAG
                     SITQGVGQFCTNPGLIIGMKGDGLDRFIAELGTAIANAAPGTMLHPGIAKAYTEKRGA
                     ALAQTDVATVAVSATNPGADQGVPTLASASAEAFFRNPLLHQEVFGPYSLIVQCRTPA
                     EMLQVARHMEGQLTTSLFATDQDLHEHADLVQAVEDICGRLILNGVPTGVRVALAMQH
                     GGPFPATTDSRFTSVGADGMRRWARPLCYQDWTDYLLPDALKNSNPLGIWRTVNNKLT
                     RDPI"
BASE COUNT         1042 a         1411 c         1526 g         1007 t
ORIGIN      
        1 ttcgtgatct tcatcgcgac cgtcggccgg cccatcaccg gatgcatgaa gaatccgcta
       61 cttcccaaaa tcgattcgac ggacacgacg ctgcaccgga accagtacga cagcgccgcg
      121 ttctatgtca gcatcctgat caacatttac aacaccagcg gcgtcttcaa tgatacgttc
      181 tcggatgacg caagcatgaa gatctatgtg gtgaatggcg tcgtgacgat ccctggcgac
      241 agcattttaa attttccgcc gatcgtattt cctcagtcgg gacaggatgg aatctataag
      301 gcgacctgga tagcggatca gatcggggtc tgtaatatta ccggcgcgta cggcattgcg
      361 gcgcccgggg acagcgcggt ctcaatcatt ctgcaacaaa cgggcacggt gacgccgaaa
      421 tggcagctcg tcgcgctggc agatggtacg accacctacg caggtggcga atccgtgccc
      481 ggctggcctc cgggatttac gttcgacccg cgaaaacagt ctcaggacgc ctttaagctg
      541 gagcaacccg ggagttattg gaacgtctgg gtgtacaagg attattgaag aaggcactat
      601 ctttaccgga tgccattact cgtgcttgtc cggcatggac aatcggtttg gaaccttcaa
      661 aatcgtttta cgggcgagac cgatgtcgac ctgacggaca agggccggat ggaggcctgt
      721 gcggcgggcg acaaactgaa gggattacgg ttcgcccacg cttttacgtc tgtcctcgta
      781 cgggcaatag aaacgctgga gctgatcttg cggacttcgg gccagccgga ctgcccggtc
      841 acgcgggacc gcgcgctcaa tgaaaggaat tatggccgcc tgcagggatt gaacaaagcg
      901 gaagtagcga aggaatacgg cgcggaacag gtggcagagt ggcggcgcag cttctctgtc
      961 cggccgcccg gcggcgaaag ccttgcggat accgctgccc gcgtccttcc cttctaccat
     1021 acgagtatcg agcctatgct gaaggacgcg aaagatatcc ttgtcgtggc gcatggaaac
     1081 agtctccggg cactggtgat gtggctcgaa aaaatcagcg aaaccgcgat cacagggctg
     1141 gatctgccaa cggggatacc gcggcggtac gagctggatg cgtcattaag gatcgtgagc
     1201 gtggggtacc tgtaaaggca gcaaggcgtt cacttcattt cgagaatcag gaatgactcg
     1261 gcgagcgccg gcgattgcgc atacaggctc ccgataaaat ccgggccata ggctgcgtac
     1321 cagggcagga tattttcgat gcgttcctgc aattgcccgg cggggaagag ggcgtctttg
     1381 agcgtttgaa cctgacggcg gaagtcgccg aattttcgtt tttcggccct aagcagtttt
     1441 ttttccaggg tccggatagg ctcgaccgcc cgggcctgca gcgcttctac gtgctgttcc
     1501 agcgaagggt cgacagggcg ggccagcgcc tttagcgact catagtagcg attggcttcc
     1561 gtgatttcgc gctcgagcga cagcgcgttc tccgattccc tgcgcacgag ctcgttaacg
     1621 agcgattcgg caggccggaa aatgtcggca atgctaaagc ccgcacggtc cattttttcc
     1681 ttccattttc gttgtatcag caaaaaagaa ttccgcagga ggagcaccgg gaaagggacc
     1741 cggtagtgtt cgaaaagaga ccggagctcc agccagtaag cggtctcgcc tcccccgccg
     1801 ataaaagcca ggttaggcag gatggtctcc tggaagagcc cccgcagaat gacgttcggg
     1861 ctgaaccgat caggatgctg gtgcaattcc gccttcatct ggtcttccgt gaatcgtaac
     1921 gatgagccat gtacgacaaa agcgtcgccc tcacggtcga tccggccacg gagatctccc
     1981 ttcaggtaaa aaaggttgat gtcccggggg ttggcttgaa ccttgtactg ctccgtgatg
     2041 cgccggatcg tttcattcac gatggcggca ggttgttgcc ggaagagatc gtccccgaag
     2101 accgggacca tcaatttctt gagcgccggt cgatcggcga tgaggacgat gaggccgaag
     2161 gcgccaaacc attcgtgaat gagccggaac gtggcggtct ggatatccgg acttttgagg
     2221 taggcctctt tcaacgtcgc caccagttgc gggccatagg gctggacgga aagctggcca
     2281 tcgatgcgga acaacagctt gtcgagtccc tgggttgtca ttcggcccac cgcacccgtc
     2341 tgtcgggtat cccagaccaa ttcctcgccg ccaagatgga tttgacccaa ttcttcgagg
     2401 tccgcatctt cactgcccat ccaaaagacg ggaacgaacc ggcagtccgg atgccgggcc
     2461 gccaattcgc cggcgaggcg gatcgtgtgc aggatcttat agatgaagta aagatggccc
     2521 gtaaagatat tcggctgatg ggcggtacaa accgtgaaca cattatcctg ttccagcagc
     2581 cggatattct ccgacacccg gcttaccggt ttgaccgcgg catattgttc ctgcaaggct
     2641 tccaccaggg cggcccggtc gaccggttgt ttacggcggc cttccagcgc cgcatccagg
     2701 ccttcgcgat ccggtgcatg cacataaaat ggcctcaggg attctgcctg atccaggtaa
     2761 tcggtgatga tacgggaaaa agagccggtc tggcgaaagg gcaaatgatg tgcgatccac
     2821 tccataagcg atggtaatgg acgaaattaa cataaaaaca ttcgaattgt tcaggcgtaa
     2881 tgatgggcgg cttggaccgg ccgcgcgcct gcggcgcgcg cgggaatacc cggcgcagca
     2941 gattggaccg gcaggcagtt acaggacctg aaaaccgtaa gcatagggat catcgtccgg
     3001 atcgatggta atggtattga atccgtagat ctttgcccag ccttcgatgc tgggaacgat
     3061 ggccggccgg cctcccacgg tggtggcctg ctcaacgcgt ccggtgaacc gcgaaccgat
     3121 gatgctttcg tggatgaacg ggtcgccaac tcgcagccgg cctttggcaa accattgggc
     3181 catgcgcgcg gaagtaccgg tgccgcacgg cgaccggtca atcgctttat cgccgtagaa
     3241 gacggcattg cgggcagtag cgtccggatg gatcggctgt cccgcccaga ggatatgcgt
     3301 gcagccatgg atggtaggat cgtcaggatg aacaaacgga tgggtttcat tgatgcggcg
     3361 gcgcattacg cgactccagc tgatgagctg gctcgccgag tagtcgtcga gcttgctgaa
     3421 attctcctgc ggatcgacga tcgcataaaa attaccgcca tatgcgacgt caacatgaag
     3481 cctgcccagg tccgggcatt ccacgggaac cccttccgcg gcgaggtaag cgggtacgtt
     3541 cgtgagtttc acggaacgga ccttattgcc ggcctgcagg tattcgatct gcacgaggcc
     3601 ggccggcgcc tccatgcgca cgacaccggg tgtctttggc tggatcagcc cttcctcgat
     3661 ggcgattgta atggtgccga tagtcccgtg gccgcacatc gggagacagc cgctggtctc
     3721 gatgaaaagt accgccacgt cattcgcggg gtcatgcggc ggatagagga tgcttccgct
     3781 catcatatca tggccgcgcg gttcgaacat caggcccttg cggatccagt catattcctt
     3841 caaaaaatgc tggcgctttt ggctcatgtt gctgccctcc agcaccggcc cgccgccggc
     3901 cacgaggcgg acggggtttc cgcacgtatg cgcatcgata caaaaaaatg tctttttcgg
     3961 catggctgtt agatcgggtc tctcgtaagc ttgttgttga cggtgcgcca gatgccgaga
     4021 gggttgctgt tcttcagcgc atcgggtaac aggtaatccg tccagtcctg gtagcacagc
     4081 ggccgcgccc agcggcgcat gccatccgcg ccgacggaag taaaacgact gtcggtagta
     4141 gcggggaacg gaccaccgtg ctgcattgct aaagccacgc ggacacctgt cggaacgcca
     4201 ttgaggatca gccgcccgca gatatcctcg actgcctgca cgaggtcggc atgctcatgc
     4261 aggtcctggt cggtcgcgaa cagggaggtt gtcagctgcc cttccatatg gcgggccacc
     4321 tgcagcattt ctgccggcgt tctgcattgc acgatgagcg aataggggcc gaacacttcc
     4381 tggtgaagca acgggttccg gaagaacgct tccgccgaag cggaagcaag ggtgggtacg
     4441 ccctggtcgg cgccaggatt ggtcgcggag acggctacgg tggcgacatc ggtctgggcg
     4501 agcgcagcac cgcgcttttc ggtataggct ttcgctatcc cagggtgcaa catggtcccg
     4561 ggcgcggcat tggcgatggc ggtaccgagc tccgcgataa aacgatccag tccgtcgccc
     4621 ttcattccaa tgatcaggcc tgggttggtg cagaactggc cgacgccctg ggtaatcgaa
     4681 ccggcgagca gggcagccac ttccttcggg ccggagcgca gcttgccggg aagcagaaaa
     4741 accgggttga tgctgcccat ttccgcgaag accggaatgg ggtcttttcg ctggccggcc
     4801 cagtcgaaaa gggccttccc gccgccgaac gaaccggtaa acccaacggc ccgggtatgg
     4861 cgatgcgtga ccagcgcgcg tccggtttca ttccccgcgc cgtgtacatg cccaaaaacc
     4921 ccggcaggca gtccggcccc ttcaacggcc ttgcggatcg cctgtgcaac gatctccgag
     4981 gtctct
//
LOCUS       DATGKE010000574         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_323952, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000574 DATGKE010000000
VERSION     DATGKE010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323952"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VKU83_09750"
     CDS             <1..298
                     /locus_tag="VKU83_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93884.1"
                     /translation="IYIIKLLSKNVIRMSQQESVAPELSPKRKVRKEIGEKVAGALAD
                     YKQELGEKVLATHVRHFSKLISRDLKKVSKKREKTAKKARAKKARSTAPKAAKQ"
     gene            complement(356..730)
                     /locus_tag="VKU83_09755"
     CDS             complement(356..730)
                     /locus_tag="VKU83_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HLI93885.1"
                     /translation="MSSEIRKIKDEDLEKIIGQLLRFGVLLSSMVVLAGGIGYLVRHG
                     LQIPSYHTFTPEPEKMRRPVPMWKAILHGEGRPLIAFGLLLLILTPIARIVFSIIGYL
                     LEKDYLYVVITVLVLIVILWNL"
     gene            complement(730..1566)
                     /locus_tag="VKU83_09760"
     CDS             complement(730..1566)
                     /locus_tag="VKU83_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLI93886.1"
                     /translation="MTVLLLSGLLFVGALTAGLLGSLTGLGGGVVLIPLLTLLFKVDI
                     HYAIGASLVSVIATSSGAAAAYVRDGISNIRLGMFLEVATTAGAVVGASLSAVLSTSV
                     IAMVFGGVLIISAVLSFLRHQDNLVVGEGSKLARILKLGSSYPTPEGRQPYTVKNIAG
                     GFVLMNIAGILSGLLGIGSGALKVLAMDNVMRTPFKVSTTTSNFMIGVTAAASAGIYL
                     RKGYIDPGLSMPVMLGVLAGAYAGSRILTRTKVSSLKRIFAIVILVLAVEMIYNGIHH
                     SI"
     gene            1661..2050
                     /locus_tag="VKU83_09765"
     CDS             1661..2050
                     /locus_tag="VKU83_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93887.1"
                     /translation="MFTAGHHAGRWIAKIFLSAICCSLLTVQSSYRFYQYANARHGRL
                     VCPGGVTRIVNACKAGRAESARARLSFRSLDKRFELEPFFLLPVPAPAPALWVQAAAR
                     RCPFFATVLIGRSHPGRCLRGPPPIFS"
     gene            2115..>2510
                     /locus_tag="VKU83_09770"
     CDS             2115..>2510
                     /locus_tag="VKU83_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93888.1"
                     /translation="MKTRNLTRDDLEGARAEMFHMILFAMAWVTIGEYTVHFRDFLVP
                     AVLVLIAVIVLGQRSTRFYELEGELPEAPAETGSEGRARKRTFQRYLWMFLLEGIAIM
                     ATWMVLLRVGHQEWLVSAFALIAGLHFFPL"
BASE COUNT          576 a          700 c          689 g          545 t
ORIGIN      
        1 aatttatatt atcaaattat tatctaaaaa cgtaattcgt atgagtcaac aagaatcggt
       61 tgctcctgag ctatcgccca agagaaaagt gcgtaaggag atcggggaga aggtggcggg
      121 cgcgctggca gactacaaac aggagctcgg cgaaaaagta ttggcaaccc atgtccggca
      181 tttcagcaaa ctgatcagcc gcgatctgaa gaaggtcagt aagaaaaggg aaaaaaccgc
      241 caagaaggca agggccaaaa aggctcgcag caccgccccg aaggcggcca aacaatagtt
      301 cgcttcttcg tgttcatgaa ttttccgtcc gtgacccgga cggatttttt ttggactaca
      361 ggttccagag gatgactatc agcaccagca ccgtgatcac cacatagaga tagtcctttt
      421 cgagcagata accgattatc gaaaagacaa tgcgcgcgat cggtgtgagg ataagcagta
      481 ataacccgaa tgcgatcagc ggcctgcctt caccatgaag aatggccttc cacatcggga
      541 ccggccggcg catcttttcg ggttcaggcg tgaaggtatg gtaggagggg atttgtaatc
      601 catgccggac gaggtagccg atacctcccg cgaggacgac catcgatgag agcaggacgc
      661 cgaagcgcag gagctgcccg atgatttttt ccaggtcttc gtcttttatt ttcctgattt
      721 ccgagctcat tagatcgaat gatggattcc attgtagatc atttccaccg caagcaccaa
      781 tatcactatc gcgaagatcc gtttgaggct tgaaaccttt gttctggtca gtatcctgga
      841 gccggcatat gcgccggcga ggactcccag catgacaggc atggagaggc cggggtcgat
      901 atagcccttc cgcagataga tccccgcgct cgcggcagcc gttactccga tcatgaagtt
      961 gcttgtagtc gtggatacct tgaaaggtgt ccgcatgaca ttgtccatgg ccaggacctt
     1021 cagtgcgccc gaaccgatcc ccagcaggcc ggatagaatg cccgcgatgt tcatcagtac
     1081 gaaaccgccc gcgatgtttt tcacggtata cggctgacgg ccttccggag tgggataact
     1141 gctgcccagc ttcagtatcc ttgcaagttt gctgccctcc cctacgacca gattgtcctg
     1201 gtgccggaga aaagacaata ccgccgaaat aatcaatacg ccgccgaaca ccatggcgat
     1261 caccgacgtc gacaacaccg ccgaaaggct tgcaccgaca actgcgccgg ccgtggttgc
     1321 gacctcgaga aacattccca gtcttatatt gctgatgcca tccctgacat aggccgctgc
     1381 cgcaccggac gaggttgcga tcacgctcac cagtgaagcg ccgatggcgt agtggatatc
     1441 caccttgaac agcaaggtca gcaggggtat cagcaccaca ccgccgccaa gccctgtcaa
     1501 tgagccaagc agtcctgcgg tcaaagcccc cacgaataat aacccggaca ataggagaac
     1561 ggtcacggtc ggcttgaaat ttgcgcaaac ctacatcatt ttttgcgcgc ctgctttttg
     1621 tcgcctaaac tcttgggaaa aagcgctaaa ttgaccggga atgttcacag ctggtcatca
     1681 tgcgggtcga tggatcgcga agatcttcct gtcggcaatt tgctgctcac tccttactgt
     1741 ccagtcctcc tatcgctttt atcaatatgc gaatgcccgg cacggcaggc tggtttgtcc
     1801 gggcggggtt actcgcatcg tcaatgcatg taaggccggt cgtgcggaaa gcgcccgtgc
     1861 ccggctgagc ttccggtcgc tcgacaaacg ctttgagctg gagccattct ttctcctgcc
     1921 cgtaccggcg ccggccccgg cgctatgggt gcaggcagcg gcccgccgct gcccgttttt
     1981 cgccacggtc ctcatcggcc ggtcccatcc cggccgctgc ctgagagggc cgccgccgat
     2041 tttctcctga tccggatggt agattcagcc aacgatgtta cggcgggata acccggcgtg
     2101 agaaacctat aaatatgaag acaagaaatt tgaccaggga tgacctggaa ggcgcccggg
     2161 cggaaatgtt ccacatgatc ctctttgcca tggcctgggt gacaatagga gaatatacgg
     2221 tgcacttccg ggacttcctg gtaccggcgg ttctcgtgct gatcgctgtc atcgttctgg
     2281 ggcagcgatc caccaggttt tatgagctgg agggcgaatt gcccgaagcg ccggcggaga
     2341 cgggatccga aggccgggcg cgaaaacgaa cctttcaacg atacctctgg atgttcctcc
     2401 tggaaggcat cgccatcatg gcgacgtgga tggtcctttt gcgcgtaggc caccaggaat
     2461 ggctggtttc ggcatttgcg ctgatcgccg gcttgcactt ctttcccctt
//
LOCUS       DATGKE010000575         4416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_344316, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000575 DATGKE010000000
VERSION     DATGKE010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344316"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VKU83_09775"
     CDS             <1..214
                     /locus_tag="VKU83_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93889.1"
                     /translation="YDFGKLIAGQQVVKGLRLTAMCNNALMFRPKENNFTDPEFNYSN
                     SNGYGNNTYYQLPPTRQYTVVAAVTF"
     gene            236..1840
                     /locus_tag="VKU83_09780"
     CDS             236..1840
                     /locus_tag="VKU83_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HLI93890.1"
                     /translation="MKKILFIFAAGCALLGSGCSKHYLSQDLNNPNQPTTDVATPQLI
                     LPAAITNLVNIVNDVTGTGGNPSYESEAVWEGYWNYQPGYSLNSAVAGYIMTSNSPQL
                     WDNYYGVLTNLNFLIQESAKTPADADYKDIAEVLEAICFQNLVDLYGDVPYSQALSVQ
                     TTFYPKYDKGSDIYDSLTAKLDAAMADISANLNNAAVTAPTNDDVMYGGNMQNWLQFA
                     NTVKLRLLLREINVTSKASYLSGEVAKTASYGYITSDALVNPGYTAADPNQIWGGFGV
                     SPSGSLNYAASFIGANGTAINFYKMTNDPRLAYFYTPNGVTANQPAFFNVTLPVNKND
                     YFAQPLGSQDKNPSGAGSSNIGPGIAQNPSQPAVMMLAAESYFLQAEATVYGWLPGGN
                     AAAQTLYQTAITKSFEYLNVGGSTAAADTYAATYYGQNLPWVAFPTGASTDSLIHTII
                     EQKWAALNGISNAEAYDDWRMTYDPTLNTGWPMVPVSIYNGNSNPHMPFRYLYPTEEQ
                     NNNNAAWTAAGGPSVDPFNTKIFWMP"
     gene            1861..2391
                     /locus_tag="VKU83_09785"
     CDS             1861..2391
                     /locus_tag="VKU83_09785"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HLI93891.1"
                     /translation="MKRNMFIAKGWPLMAGAAMALFFLSSCLKSGPYNYDFAGVGASV
                     DLPLAAANGNGVVPFTFGAGSNTFPVYADLASPSPLSKSTTVTLMVDSAYLNSYNANN
                     GTGYSLLPDSDYTINTLNLTIPAGQRLDSALVTFNIAKVDTSSAISYVLPISISSASE
                     PVEQWSHLLIGVTVVQ"
     gene            2477..2788
                     /locus_tag="VKU83_09790"
     CDS             2477..2788
                     /locus_tag="VKU83_09790"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HLI93892.1"
                     /translation="MAEQTPNNQLNIEISEEMADGQYANLAIITHSHAEFVIDFVNVM
                     PGTPKSKVRARIILTPQHAKRFMKALTENVGRFEAANGKIQDLEEVQLPLNFGGPTAQ
                     A"
     gene            complement(2858..3577)
                     /gene="radC"
                     /locus_tag="VKU83_09795"
     CDS             complement(2858..3577)
                     /gene="radC"
                     /locus_tag="VKU83_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLI93893.1"
                     /translation="MQAKAPAIAEKSRPIKDWAEGDRPREKLILKGPEVLSDSELLCL
                     LIGKGTRRQNAMEMARDLLAQNGNSLQALARRGINDFLQLRIKGFGKAKACSISAAFE
                     LGRRRAAGLSPEKPVFKDSKAAAEYLQPLLADFSHEVFYVLYLDQANGLIDCKRVSEG
                     GLTATYVDPRVIFKKALQEEAVSIIVSHNHPSGNLRPSRADEVLTARLKDGANYMDIK
                     LLDHIIVSPNGYFSFAAEGLL"
     gene            3706..3909
                     /locus_tag="VKU83_09800"
     CDS             3706..3909
                     /locus_tag="VKU83_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93894.1"
                     /translation="MKRSTFNAIGGWQPLFFCVGMYVVALFFSIFVCSSIFYAVNSRP
                     AAKAEKTAVANVARNPQQLASVN"
     gene            4124..>4416
                     /locus_tag="VKU83_09805"
     CDS             4124..>4416
                     /locus_tag="VKU83_09805"
                     /inference="COORDINATES: protein motif:HMM:NF025165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HLI93895.1"
                     /translation="MQHLAKIFSGTGSQYLTEKIAQQFGHPAGKISIQRFSDGEIQPV
                     FNESIRGDMVFLIQSTCAPAENLLELLLMIDAARRASAYKVVAVIPYYGYARQ"
BASE COUNT         1013 a         1217 c         1205 g          981 t
ORIGIN      
        1 ctatgatttc ggcaagctga tcgcgggcca gcaggtagtc aaggggctgc gattgacggc
       61 aatgtgcaac aatgcactga tgttcagacc gaaggagaac aatttcaccg atccggagtt
      121 caactacagc aacagcaatg gttacgggaa caatacctat taccagttgc cgccgacccg
      181 gcagtacacc gtagttgccg ccgtaacctt ttaatttaac caatcaagac atttcatgaa
      241 aaagatatta ttcatatttg ccgccggctg tgcgttgctg ggaagcgggt gcagcaaaca
      301 ttatctgtcc caggacctga ataatccgaa ccagccgacg acggacgtcg cgacgccgca
      361 gctcatcctg ccggcagcca ttacgaacct cgtcaatatt gtcaatgatg ttaccggcac
      421 aggggggaat cccagttacg agtccgaggc ggtttgggaa ggctattgga attaccagcc
      481 ggggtattcc ctgaattcgg ccgttgcggg ctacatcatg acatcgaact cgccgcaact
      541 ttgggataat tactacggcg ttctcaccaa cctgaacttc ctgatccagg aatcggcgaa
      601 gacgccggcc gatgcggatt ataaagatat cgccgaagtg ttggaggcga tctgtttcca
      661 aaacctggta gacctgtatg gagacgttcc ttattcccag gcgctgagcg ttcagaccac
      721 gttctatccg aaatacgaca agggcagcga tatctatgac agcctgaccg ctaagctgga
      781 tgctgccatg gccgatattt cggccaacct gaacaacgcc gcagttacag ctccaacgaa
      841 cgacgatgta atgtatggcg gcaacatgca gaattggctg cagtttgcca acacagtgaa
      901 gttgaggctg ctgctccggg aaataaacgt tacgtcgaaa gcatcctatc tttccggcga
      961 ggtcgccaaa acggcttctt atggttatat cacgtcggac gcgctcgtga atcccgggta
     1021 cacggcggca gatccaaacc agatctgggg cggattcgga gtgagccctt ccggctcgct
     1081 gaactatgcc gccagcttta tcggcgcgaa cggcacggcg atcaacttct acaaaatgac
     1141 caacgatccg cgcctggcct atttctatac gccgaatggc gtgaccgcta atcagccagc
     1201 cttctttaac gttacccttc ccgtcaataa gaacgattat ttcgcccagc cgctgggcag
     1261 ccaggacaag aaccccagcg gagcgggttc gtcgaatatc ggcccgggga tcgcgcaaaa
     1321 tccctcgcag cccgcagtca tgatgcttgc cgcagaaagc tatttcctgc aggccgaggc
     1381 gacggtttac ggctggttgc cggggggaaa cgccgctgcg cagactttgt accagacggc
     1441 catcaccaag tccttcgaat acctgaatgt tgggggaagt accgctgccg ccgacaccta
     1501 tgccgctacc tattatggtc agaatctgcc ctgggtggct ttcccgaccg gggccagcac
     1561 cgatagcctg atccacacga tcatcgagca gaaatgggcg gcgctaaatg gcatcagcaa
     1621 cgcggaagcg tatgacgact ggcggatgac ctatgatccg acattgaata caggatggcc
     1681 catggtgccg gtatccatct ataacggtaa ctccaacccg catatgccgt tccgttacct
     1741 gtacccgacg gaagaacaga ataacaacaa tgcggcctgg accgctgccg gcggcccttc
     1801 ggtcgatcct tttaacacaa aaatattctg gatgccctaa cccggcaaaa aatgaaaatt
     1861 atgaaaagaa acatgtttat agcaaaggga tggccgttga tggcgggcgc ggcgatggcg
     1921 ttgtttttcc tgagctcatg cctgaagagc ggcccgtata attacgattt cgccggtgtc
     1981 ggcgcttcgg tggacctgcc gcttgccgca gcaaacggaa acggggttgt cccctttacc
     2041 ttcggcgccg gctccaacac cttcccggtg tatgcagatc tggcaagtcc ctcgccgttg
     2101 agcaaatcca cgacggtgac cctgatggtc gattcagcct accttaactc gtacaacgct
     2161 aataacggaa ccggatactc gctgcttccg gacagcgact acaccatcaa cacactgaat
     2221 ttgaccattc cggccggcca gcggctggac agcgcgctgg tgaccttcaa tatcgcgaag
     2281 gttgacacca gctccgccat ttcttatgtg ctgccgatct ccatatcgtc ggccagcgag
     2341 ccggtcgagc aatggagcca cctgctgatc ggggtgacgg tcgttcaata ggcagataaa
     2401 aaggccgtct caccgggatc gaggcggcct ttttttgccg acgcaaaccc ggcgtatctt
     2461 tgcccaaaat cacttcatgg cggaacagac acccaacaat cagctgaata tcgagatcag
     2521 tgaggagatg gccgatggcc agtatgccaa tctggccatt atcacccatt cgcacgcgga
     2581 attcgtgatc gatttcgtaa acgtcatgcc gggcacgcca aagagcaagg tgcgggcccg
     2641 catcatcctg accccgcagc atgcaaaacg gtttatgaag gcgctgaccg aaaacgtggg
     2701 ccggtttgag gccgccaacg ggaagatcca ggacctggag gaagtgcaat taccgctgaa
     2761 tttcggcggc ccgacggccc aggcctgagg atgcggggcg ggtgggctca tcagtagtgg
     2821 gcgggcgacg gtagccgggc taaggtgcct tgtgggatta cagcagacct tcggcggcaa
     2881 agctgaaata gccgttcggg ctcacgatga tatgatccag tagtttgata tccatgtagt
     2941 tggcgccatc cttaagcctg gcggtcagga cttcatcggc gcggctgggc cgcaggttgc
     3001 cggatggatg gttgtgactg acgataatac tgactgcttc ttcctgaagg gcttttttga
     3061 agataacgcg aggatcgaca taagtggcgg tgaggccgcc ttcactgacc cgtttgcaat
     3121 ctatcaggcc gttggcctgg tcgagataca ggacatagaa tacctcatgc gaaaagtcgg
     3181 cgaggagcgg ctggaggtat tcggcggcgg ctttgctgtc tttaaagaca ggcttttccg
     3241 gcgacaggcc ggcggcgcgg cgccgcccga gttcaaaagc cgccgatatg ctgcaggcct
     3301 ttgcctttcc gaatcctttg atgcgcagct gcagaaagtc gtttattccg cgcctggcca
     3361 gggcctggag actgttcccg ttttgggcaa ggagatcgcg ggccatctcc attgcgtttt
     3421 gacggcgggt gcccttgccg atgaggagac agagcagctc cgaatcgctg agcacttcgg
     3481 ggcccttgag aatcaacttc tcacgaggcc ggtcaccttc ggcccagtcc ttgatggggc
     3541 gagatttttc cgctatggca ggcgctttgg cttgcatttt tcaatattaa atattatttt
     3601 gtcaatcaaa gcgggaaaat gaaatactga agggatttcc ccgtttatcc gtacggctaa
     3661 atctgagcgc gagctcagcc tttgaaaact attaatccaa attcaatgaa acgctcaacc
     3721 ttcaatgcaa tcggtggctg gcagcccttg ttcttttgtg taggaatgta tgtagtcgcc
     3781 ctctttttct cgatcttcgt ttgttcctcg attttttatg ctgtcaattc aagaccggcc
     3841 gcaaaggcgg agaagactgc agtcgctaac gttgccagga atccgcagca gcttgcttcc
     3901 gtgaattaag gtacctggca gaatctgcca cgcctccggc atcatgccgc gccgccccga
     3961 gccggcatac catgaccccg gctgaaatgt attgcaggcc gcccgcccgc gcgggccggc
     4021 cttttccgct tttcaaagag atttgcgcga gcgctttttg ttctttgcgc cccgttaggt
     4081 tgtaagtgga atccttatct tcgcgccata ttttatcttc attatgcagc acttagccaa
     4141 gatcttttcc ggcaccgggt cacagtacct gaccgaaaag atagctcagc aattcggtca
     4201 ccctgcgggc aaaatttcta tccagcgatt cagcgacggc gagattcaac cggtattcaa
     4261 cgaaagcatc cggggagaca tggtattcct cattcagagt acctgcgcgc cggcggaaaa
     4321 cctcctggaa ttgctgttga tgatagatgc cgcgcggcgc gcctcagctt acaaggtggt
     4381 ggccgtaatc ccctattacg gatatgcgcg gcagga
//
LOCUS       DATGKE010000576         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_99908, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000576 DATGKE010000000
VERSION     DATGKE010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99908"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VKU83_09810"
     CDS             <1..456
                     /locus_tag="VKU83_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93896.1"
                     /translation="DRKFDEIIYMTPDGERLNQKIANHLSLKNSILIICGHYKGIDQR
                     IRERYVTRELSIGDYVLSGGELAAAVLADAVGRLIPGVLNDETSALFDSFQDNLLAPP
                     VYTRPADFRGAKVPEVLLSGDPRKIDAWRHDQSVTRTQQRRPDLLADDE"
     gene            829..1776
                     /locus_tag="VKU83_09815"
     CDS             829..1776
                     /locus_tag="VKU83_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93897.1"
                     /translation="MTISGFTMVRNATKLYYPIKQTISSILPLVDEFVVALGDCDPDD
                     RTLEEINIIGSDKIRIIHTVWDLDKYPRGMEHAHQTDIAKAACTGDWLFYLQSDEVVH
                     EDDLPKIRLRCEELLHTPEVEGILFKYRHFWGDYDHYITSHAWYANEIRIIRNDREIH
                     SWESAQSFRRIPGFDGVHYREKKGTYKLKVARVDAHIFHYGWVRPPHYMQKKRKAFAT
                     IHSGRDKAEEYFRREQEDFDFGNLSQLSVYQDPHPAVMQERIRLFNWADLLERHRYRQ
                     TITRHKHERFKYRLLTFIEQNLLGGRQIMSFKNYRLLSR"
     gene            complement(1848..2564)
                     /locus_tag="VKU83_09820"
     CDS             complement(1848..2564)
                     /locus_tag="VKU83_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93898.1"
                     /translation="MEALIKRSFFVLAATTLSLTTLRAQTADDIVNKYVAAIGGKDAL
                     NNLHSLIIQATTSVMGNDGDTQDTIVFGKGYKNHSDFGGTTLVTCITPNGGWTINPFM
                     GASTPTALPEDQLKSQQINLTLDPLANYAANGNTIVLDGQDSADYKIKMTGKGPEMTY
                     FINMKTYLIDKVTTHMSMGGQDADVNIGFSDYRKLPDVGLMYPYAENLDLPQASLAIT
                     IKKVTVNPTVDPSVFDMPKQ"
     gene            2674..2904
                     /locus_tag="VKU83_09825"
     CDS             2674..2904
                     /locus_tag="VKU83_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93899.1"
                     /translation="MQRDIFRRLDRIDHLIRIKGTGTPAQFADKVGLSERTMYAYLRL
                     MKDFGAPVVYSRQKQSYYYLRGGRFIVRFLSE"
     gene            complement(3019..>3663)
                     /locus_tag="VKU83_09830"
     CDS             complement(3019..>3663)
                     /locus_tag="VKU83_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HLI93900.1"
                     /translation="LDSFGGHNYLYAANFMTGNVDVFDTAFKLAPLSFVDPALPAGYA
                     PFNIKAIGSWLFVNYAERTPGSIDEMHGPGLGIVDVFSPDGTFVRRFASHGSLNAPWG
                     IAWAPAGWLSKEDMDTTGGKGMGSDMDHADHGHDVSTAAVLIGNFGDGHINVYAPDGS
                     FLGQLRSHNKVIAIDGLWALSFPPATSSIDQNRLYFTAGPADEADGEFGYLIKQ"
BASE COUNT          888 a         1000 c          936 g          839 t
ORIGIN      
        1 gaccggaaat ttgacgagat catttacatg acacccgacg gtgaacgact aaatcaaaaa
       61 attgccaacc acctaagctt aaaaaacagt attctgatca tctgtggcca ctacaagggt
      121 atcgaccagc ggatccgtga gcgatatgtt acgcgcgagc tttccatcgg cgactacgtc
      181 ctgagcggcg gcgaattggc cgcggctgtc ctggccgatg cggtcgggcg cctgatcccg
      241 ggcgtgctga atgatgaaac ttccgccctc ttcgattcct ttcaggacaa tttgctggcg
      301 ccgccggtat atacgcggcc ggctgatttc cgtggggcaa aggtccctga agtgctcttg
      361 agcggcgatc ccaggaagat cgacgcctgg cggcatgatc aatctgtcac ccggacccaa
      421 cagcggcggc cggacctctt ggcggacgat gaatgaggca aaaccgcccc gcgtgtaccc
      481 attgacggct tcgcgacagc cgcctcgtcg gcgcccgatt accggaaaat catcgccagc
      541 cagacaagta attgaaggac aagataatac ccaagaataa gcagtagatt aaagacaata
      601 gtctttctca taaggaacgg atttaaatgc ctttaaaggc ccaaaaccca taccaaatgc
      661 agcttttcca tatcggattt ctactcctaa agccttgaaa attcgcccgg taatctccca
      721 ttaagcaacg tttaatgacc tcttaatctt atcggaataa ccctgcacgg aaattgtaag
      781 tcaatacttt gaaccaatga tcattcaata agcttaaaaa ccggcataat gaccattagc
      841 ggattcacga tggtgcgcaa cgccaccaaa ctttactacc ccattaaaca gacgatctct
      901 tcaatcctac cccttgtcga cgagtttgtg gtcgccctgg gcgactgcga ccctgacgac
      961 cgcacgctgg aagaaatcaa catcatcgga tcggacaaga tcaggatcat ccacaccgtc
     1021 tgggacctcg acaaatatcc ccgcggcatg gagcatgccc accagaccga tatcgccaaa
     1081 gccgcctgta ccggcgactg gttgttctat ctccagagcg acgaagtcgt ccatgaggac
     1141 gacctcccga agatccggct tcgttgcgag gagcttctcc atacgcccga ggtagaaggc
     1201 attttattca aataccggca tttttggggt gactatgacc attacatcac gtcacacgcc
     1261 tggtatgcga atgagatccg gatcatccgg aacgatcggg aaattcattc ctgggaatca
     1321 gcgcagtcgt tccgccggat tccaggtttt gatggcgtcc attaccggga aaaaaagggc
     1381 acctacaaac tgaaagtggc ccgggtcgac gcccatatct ttcactatgg ctgggtgcgc
     1441 ccgccacatt acatgcagaa aaagagaaaa gcctttgcta cgatccattc gggccgcgac
     1501 aaggccgaag agtatttccg ccgcgagcag gaggactttg attttggtaa tttaagccag
     1561 ctatcggtct accaggaccc gcatcccgcc gtgatgcagg agcggatccg cttattcaac
     1621 tgggccgacc tgctggagcg acaccggtac cggcaaacca taacgcgtca caaacatgag
     1681 cgcttcaagt accggctgct gacctttatc gagcaaaact tgctgggcgg gcgccagatc
     1741 atgtccttca agaactaccg tcttttgagt cggtgatgta gattgaagcc aaagaaaaac
     1801 ggggcctgaa ggccccgctg catttataga gtgtgaatcg aaaaaggcta ttgtttcggc
     1861 atgtcgaaga ccgagggatc gaccgtggga ttgaccgtta ctttcttgat tgtgatggca
     1921 agcgaagcct ggggcaggtc caggttctct gcgtaggggt acataagccc gacgtccggc
     1981 agcttgcggt aatccgaaaa gccgatattg acatcagcat cctggccgcc catggacata
     2041 tgggtggtca ccttgtcgat gaggtaggtc ttcatgttga taaaataagt catttcagga
     2101 cctttgcccg tcatctttat cttgtagtcc gcgctgtcct ggccatccag cacaatcgtg
     2161 tttccattgg ccgcgtagtt ggccagcggg tcgagcgtca ggttgatttg ctggcttttc
     2221 aactggtctt ccggcagggc tgtgggagtg ctggcgccca tgaacgggtt gatcgtccaa
     2281 ccgccgtttg gcgtaatgca ggtgaccagt gtggtcccgc caaaatccga gtgattcttg
     2341 tagcctttgc cgaagacgat ggtatcctgc gtatctccgt cgttgcccat tactgacgtg
     2401 gtggcttgaa taatgagcga atgaaggttg ttgagggcat cctttccgcc aatggccgcg
     2461 acatatttat tgacgatgtc gtccgccgtc tgggctcgga gggtcgtcag gctgagtgtg
     2521 gtggcggcca gtacaaaaaa cgaacgtttg ataagtgctt ccattgattt agttttatat
     2581 aaaatatcta taatacctca aacataaaaa ttatttcctt caaccgcttt accttccata
     2641 aaataacctt atcaagtaac caaaccttat tctatgcaaa gggatatttt caggcggctg
     2701 gaccgcattg accatcttat ccgcatcaag ggcaccggaa ctccagccca gtttgccgac
     2761 aaggtcggcc tttccgaacg aaccatgtac gcataccttc gcctcatgaa agactttggc
     2821 gcgccggtag tctattcccg gcaaaagcag agctattact atttacgtgg cggaaggttt
     2881 attgttcgct ttttgtccga gtaagtcgaa ttatttatcc gggtaatcgc agcatgtaat
     2941 ggtaaaagcc cccacccctc ccggggcggg ggccccaact caacgttgac agcgcaaggg
     3001 ggccttcagc cccttccctt attgtttgat caaataccca aattctccgt ctgcttcatc
     3061 cgcggggccc gccgtaaaat acagcctgtt ttgatcgatg cttgaggtag ccggcgggaa
     3121 ggacaatgcc cagagtccgt cgatcgcgat aaccttgttg tgcgaccgca actgcccgag
     3181 gaagctgccg tcgggggcat aaacgttaat atgtccatct ccgaagttcc cgatgaggac
     3241 ggccgccgtt gaaacgtcgt gtccatggtc cgcgtggtcc atgtccgagc ccattccctt
     3301 cccacccgtg gtatccatgt cttccttgga tagccagccc gcgggcgccc aggcgattcc
     3361 ccagggagcg ttcaaagagc cgtgcgatgc gaaacgccgg acaaacgttc catccggact
     3421 gaacacgtcc acaattccca ggccaggccc gtgcatctca tcgatgctgc cgggagttcg
     3481 ctccgcatag ttcacaaaca accagctgcc aatcgccttg atattgaagg gcgcatatcc
     3541 ggccggcagc gcgggatcaa cgaaggacaa tggcgccaat ttgaaggcgg tgtcaaaaac
     3601 atctacattg cccgtcatga agttcgcggc atagaggtag ttatggccgc cgaagctatc
     3661 caa
//
LOCUS       DATGKE010000577         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_54919, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000577 DATGKE010000000
VERSION     DATGKE010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54919"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2493)
                     /locus_tag="VKU83_09835"
     CDS             complement(<1..>2493)
                     /locus_tag="VKU83_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI93901.1"
                     /translation="TRDFKNDIWISTWFGGIDKLNRSGNTFTHYSCYNPVQKIDERNV
                     WQVYEDGKKRLWACASNRGHLYLYNRQADKFELFDNSLIDFQVLTEDRSGNLWGGNYN
                     SLIEVDPDKREHKRYFIGYPVRSIREDRNHNFWVGTEGGGLLLFDREKGKFRQITTKD
                     GLPNNGILRILEDSRGNLWLSTYYGLCRFNPVSKTCRNFTQADGLQSNQFSFNAAMIS
                     KSGEFLFGGIKGFNVFYPDSIYEKRDIPRLFLTGLTIDNLPVETETAYVTARDKDIIT
                     GIAVPFEKAVLSFDFTALQYSNAANLKYAYYLKGWDKGWDITNNVRTANYSHLHEGDY
                     TFYVRVMNADGFWNDEVRLLSVTVLPPWYRTWLAYCLYAAVMIGAIYGFIYYYRRQDR
                     LRYEIRLAILEKQKEKELTERKIAFFTHISHEFRTPLTLIVNPLKDLMAETASMPMQK
                     KLVTVHRNARRLLSLVDQLLLFRKVESIDQQLNVTKFDLTETCDEVFLIFAQRAASKN
                     IGFTFDKPMDEVFFCGDKEKIEIILFNLLSNALKYTPAGGKVGLSLTEEDSEFCILVK
                     DTGCGIAEHVRNRLFDAFYQANNTGKASQTGFGIGLYVSQRLAAAHGGALSYRSTEGK
                     GSEFTLTLPINRTTLTGLGEEEVRCERQSIIHELVEEVREDGEKEVDNKSKVIDKIIS
                     GLPTMVIVDDDADLRSYMKEIFVDRFNIYEADDGMSAFALISKEVPDIVVSDVVMGKM
                     DGIELCRKLKENPFLAHIPVILLTGSASEQSKLKGLEGGAEDYVSKPFSKELIVARVQ
                     NILRSRSRLQQYFFNKITLKPTAGIEAGHKEFL"
BASE COUNT          603 a          678 c          535 g          677 t
ORIGIN      
        1 cagaaattcc ttgtgaccgg cctcaatgcc ggcagtgggc tttagcgtta tcttattgaa
       61 gaaatattgc tggagcctgc tccgggatcg caggatattc tgaacacggg cgacgatcaa
      121 ttccttgctg aatggcttac ttacgtaatc ttcggcacca ccctccagtc cctttagttt
      181 cgattgttcg gatgcgctgc ccgtcaacag gatgacggga atgtgggcga gaaacgggtt
      241 ttctttaagc ttacggcaca attcaatgcc atccattttg cccatcacca catcgctgac
      301 gacaatatcg ggcacttctt tgctgatcaa tgcaaatgcg gacatgccgt catcggcttc
      361 atagatattg aaccggtcga cgaagatctc cttcatgtag ctcctcaaat ccgcatcatc
      421 atcgacgatc accatcgtcg gcagccccga gatgatcttg tcaattacct tcgatttatt
      481 atcaacctcc ttttctccat cttcccggac ttcttcgacc aattcgtgaa tgatgctttg
      541 acgctcgcat ctgacctctt cttcgccaag gcccgtgagc gtagtcctgt ttatgggaag
      601 ggtcagcgta aattcactgc ctttcccttc cgtacttcta tacgacaagg ccccgccgtg
      661 ggctgccgca agccgttggg atacatatag ccctatgcca aatccggttt gggacgcctt
      721 cccggtgttg ttggcctggt aaaacgcgtc gaacaggcgg ttgcggacgt gctctgcaat
      781 cccgcacccg gtatccttca cgaggataca gaattccgag tcctcttccg tgagcgacaa
      841 acccactttt ccgcctgcgg gggtatactt caatgcattg gacaaaagat tgaacaggat
      901 gatctcgatc ttttccttgt ccccgcaaaa aaatacctca tccataggtt tatcgaaagt
      961 aaaaccgatg tttttggagg cggcccgctg cgcaaaaata aggaagacct cgtcgcaggt
     1021 ttcggtcaag tcgaatttcg taacgttgag ctgctggtct atggattcca cttttctaaa
     1081 caacagcaat tggtcgacca gactcaacag ccgccgggca ttccggtgaa cggtgaccaa
     1141 tttcttttgc atcggcatgg aggccgtctc cgccatcagg tccttcaacg ggtttacgat
     1201 caatgtaagc ggcgtcctga attcatggga gatgtgggtg aaaaatgcga tctttctttc
     1261 cgtcaactcc ttctcctttt gcttttccaa gattgccagc cttatttcat accgcagccg
     1321 gtcctgccgg cgataataat aaatgaaacc ataaatggcg ccgatcataa ctgccgcata
     1381 aagacaatag gccagccagg tcctgtacca cggcggtaag acagttacgg acaacaatcg
     1441 aacctcgtca ttccaaaacc catccgcatt cattaccctg acataaaagg tatagtcgcc
     1501 ttcatgcaaa tgactataat tagccgttct tacgttgttc gtaatatccc aacccttatc
     1561 ccatcccttt agataatacg cgtatttgag attggccgca ttcgaatatt gcaacgcggt
     1621 aaaatcgaat gaaaggaccg ctttttcaaa tggaacggct atgccggtaa ttatgtcttt
     1681 gtcgcgtgcc gtaacataag ccgtttccgt ttcgacgggc aggttatcga tcgtcagccc
     1741 ggtgaggaat aaacgcggga tatcccgctt ttcgtagata ctatccggat aaaagacatt
     1801 aaagccctta atgccgccaa acagaaattc cccgcttttt gagatcatcg cggcattgaa
     1861 gctgaactga ttgctttgca gtccgtcggc ctgggtaaaa ttcctgcacg tctttgaaac
     1921 cgggttgaaa cggcataagc cataatacgt actgagccaa aggttacccc tgctatcctc
     1981 cagtatcctc aagatcccat tgttcggcaa accgtctttt gttgtgatct gccggaattt
     2041 tcctttttcc cgatcgaata acaataaccc ccctccttcg gtgccaaccc aaaaattatg
     2101 gttcctgtct tcccggatgc tcctcaccgg ataaccgata aaatatcttt tatgctccct
     2161 tttgtccgga tcgacctcta tcaacgaatt gtaattgccg ccccacaaat taccggatct
     2221 gtcttccgtc aacacctgaa aatcgatcag cgaattgtca aaaagctcaa acttgtcggc
     2281 ctgccggtta tacaaataca gatgacccct attgctggca catgcccaca accgcttttt
     2341 gccgtcctcg taaacctgcc aaacgtttct ttcatcgatc ttttggaccg ggttataaca
     2401 gctatagtgc gtgaacgtgt tgcccgatct gttgagcttg tctatcccgc caaaccaggt
     2461 ggagatccag atatcgtttt taaagtccct ggt
//
LOCUS       DATGKE010000578         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_369178, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000578 DATGKE010000000
VERSION     DATGKE010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369178"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="rlmD"
                     /locus_tag="VKU83_09840"
     CDS             <1..1018
                     /gene="rlmD"
                     /locus_tag="VKU83_09840"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HLI93902.1"
                     /translation="TFSNRRFLLPSELHNDAVSAEQRVAGFHARGLFDKVVDIDTCHL
                     QDEPTNRIRKAVRDFGLERDLSFYDIRNHEGWLRNLQVRICTTGEIMVNLVVGYEDEG
                     LTRQLADRLVEQFPGLTTLLYTVNPKWNDSISGLTPRILHGKGYVIEKLEDFHFKIGP
                     KSFFQTNTRQAENLYQVTRHLAELTGKETVYDLYCGTGSIGIFVSRLAARIVGVDVIE
                     EAIADARENAELNGIRHARFFSGDVTDVCRDSFFEAQGRPDVIITDPPRAGMSEGLTA
                     TILEIEAPIVVYVSCNPSTQARDLAILDRKYAVTTVQPVDMFPHTHHIENIVQLKLRE
                     AATK"
     gene            1113..1817
                     /locus_tag="VKU83_09845"
     CDS             1113..1817
                     /locus_tag="VKU83_09845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLI93903.1"
                     /translation="MTEFRPGGFQLIPTVIKNLIIINVLIYLAQATLGAAFNEKMINL
                     FALHDVRSPLFRPHQLLTYLFLHGGWEHVLFNMFALWMFGSILENYWGPKRFLIFYVI
                     CGLGAAVAHLAVLYPELTPYFNDLHQVPNLDQQPDYFDPSQPWNGATLGASGAVFGCL
                     AAMGYLFPNSLVYLYFFVPIKVKWFVLGYAALELISGVQSTAGDNVAHWAHLGGGLVG
                     LLLVIYWNRTNRRRFY"
     gene            1912..2829
                     /locus_tag="VKU83_09850"
     CDS             1912..2829
                     /locus_tag="VKU83_09850"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLI93904.1"
                     /translation="MQDNYRKKMALGQDGNALVQLVVLNGVLFVILKFIYVLYLLNSR
                     NPAAYDSNVLNWFVLPGTVEKLSSRPWTVLTYMFSDQHIFRFIGNMFWLWCFGYILQD
                     MTGNRKLIPIYLYGGVAGAIFFLLSYAIFPQLQAAAPAARLIGSNAAIMALAIAITTV
                     APDYRIFPMINGGIPLWILTLLYLLVDFSSIAHGDAGAYIANLAGAATGFLFIYQLRR
                     GHDGSVWMNQVFDWFNNLFDPDKKQRIRSPKDEFYYKVSGTQPFKKIPNVTQQRIDEI
                     LDKINQQGYRFLTDEEKEVLKRAADEEEL"
     gene            2843..>3668
                     /locus_tag="VKU83_09855"
     CDS             2843..>3668
                     /locus_tag="VKU83_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLI93905.1"
                     /translation="MPKIRSYARHLFIIVNGVVVLLFLLACANGFLPPGKWWLIALLG
                     LAFPYLFLFVASFFAVTVSMPALRRWSLLSLAALLIGWSNIRHFIALHPGDRFVQQRR
                     PGTAIRILTWNVRSFDDFVTRKRPPVRHRTGMLEFIGAQQADVLCLQEFYEALTRHGP
                     ESNITYIQEQLHYPYYYFSRDYTRPGLYEAGVVIFSRFPIVDSSCHAFLKPYGFHTTE
                     SLISADLVVGADTVRVYTTHLQSVLFGSKEFRDLQIIRNVDDSIVDASRSIVRKLRE"
BASE COUNT          775 a         1051 c          964 g          878 t
ORIGIN      
        1 tactttttcc aaccgccgct tcctgctgcc ctcggagctg cataacgacg ctgtctcggc
       61 cgaacagcgg gtggcggggt ttcatgcgag gggactgttc gacaaagtgg tggatatcga
      121 tacctgtcat ttgcaggacg agccgaccaa ccgcatccgg aaagcggtca gggacttcgg
      181 cctcgaaagg gacctgagct tctacgatat ccgtaatcat gagggctggc tgcgaaacct
      241 gcaggtccgg atctgcacca ccggtgagat catggtcaac ctggtcgttg gatacgagga
      301 tgaggggttg acccgccagc ttgccgatcg gctggtggaa cagtttcccg gattgaccac
      361 tctgttgtat accgtcaatc cgaaatggaa tgacagcatc agcggcctga cgccccgaat
      421 cctgcacggt aagggttacg tgatcgaaaa actggaggat ttccacttta agatcggtcc
      481 caagtcattc tttcaaacga acacccggca ggccgaaaat ctttaccagg tgacgcgcca
      541 tctggcggaa ctgaccggga aagagaccgt gtatgatctg tattgcggca cgggcagcat
      601 cggcatcttt gtcagccggc tagccgcccg gatcgtcggc gttgatgtga tcgaagaagc
      661 gatcgccgat gcgcgggaaa atgcggaact gaatggtatc cgccatgccc gttttttttc
      721 cggcgatgtc accgacgtct gcagggacag cttcttcgaa gcgcagggaa ggccggacgt
      781 aatcattaca gacccgccgc gcgccgggat gagcgaaggg ttgaccgcta ccatccttga
      841 aatcgaagcg cctatcgtag tctatgtgag ttgcaacccg tcaacgcagg cccgcgacct
      901 tgcgatcctt gacagaaaat atgccgtaac caccgttcag ccggtggaca tgttccctca
      961 tacgcatcac atcgagaata ttgtgcaact aaagctcaga gaggcggcta caaaataaaa
     1021 aatgtaattt gcacccggac cgggcgtagc cctgttaccg ttttcgccgc aaaaattaag
     1081 gaatacagcc gcaaaaatta aggaatacat atatgactga atttcgtccg ggagggtttc
     1141 aactgattcc gacagttata aagaatctga tcatcatcaa tgtcctgatt tatctcgcac
     1201 aggctacgct tggggccgct ttcaatgaaa agatgatcaa tctgttcgca ctccatgacg
     1261 ttcgctcgcc gcttttccgt ccgcaccaac tcctcaccta tttattcctg cacggcgggt
     1321 gggagcacgt gctgttcaat atgttcgccc tttggatgtt tggttccatc ctggaaaact
     1381 attggggacc gaaacggttc ctgatatttt atgtgatctg cggcctcggc gccgccgtgg
     1441 cccacctcgc cgtcctgtac ccggaactga ccccttactt caacgattta catcaggttc
     1501 caaacctcga tcaacagccc gactatttcg accccagtca gccatggaat ggcgccaccc
     1561 ttggcgcttc cggtgcggtg ttcggctgtc ttgccgcaat gggttatctt ttccccaata
     1621 gcctcgtcta tctctacttt tttgtcccca taaaggtcaa gtggttcgtc ctcgggtatg
     1681 cggccctgga gctgatctcc ggcgtccagt cgacggcagg cgacaatgtt gcccattggg
     1741 cccatctcgg cggcgggctg gtgggtttat tgcttgtcat ttactggaac cggacaaacc
     1801 ggcgacgttt ttattaaact cttttccaaa ataagtgttt aaaaagactg aggtgacggg
     1861 attatttcat ccggcgccgg atttcgccat ccactcaatc gtatgcgggt aatgcaagac
     1921 aactatagga agaaaatggc cctgggccag gatggcaacg cgttggtcca gctggtggtg
     1981 ctcaatggag tattgtttgt gatcctaaag tttatttacg tactgtacct gctgaattcc
     2041 cggaacccgg cggcgtatga cagcaacgtg ctcaactggt tcgtgcttcc gggaacggtc
     2101 gaaaagctgt cttcccggcc gtggacagtg ctgacctata tgttctccga ccagcatatc
     2161 ttccggttta tcggtaatat gttctggcta tggtgttttg gctatatcct gcaggacatg
     2221 accggcaacc ggaagctgat tccgatatac ctgtatggcg gcgtggccgg cgcgatcttt
     2281 ttcctgctgt catacgctat atttccgcaa ctgcaggcag cggcaccggc cgcccggctt
     2341 atcgggtcga atgcagcaat aatggccctt gccatcgcca tcaccaccgt ggcgccggac
     2401 tatcgcattt tcccgatgat caacggcggg atcccgctgt ggatcctgac gctgctgtat
     2461 ctccttgttg acttttccag catcgctcat ggcgatgccg gcgcctatat tgccaacctg
     2521 gccggcgccg cgaccgggtt cctgtttatt taccagcttc ggcggggcca cgacggcagc
     2581 gtatggatga accaggtctt cgactggttc aataacctct tcgaccctga caagaagcaa
     2641 aggatccggt ccccgaaaga cgagttttac tacaaggttt ccggcaccca gccatttaaa
     2701 aagatcccga acgtcacgca gcagcgaatc gacgagatcc ttgacaagat caaccagcag
     2761 ggctaccgct ttctcacgga cgaggaaaaa gaagtcctga aacgggcggc tgacgaagaa
     2821 gaattgtaac tttgcggctg ccatgccgaa aattcgcagt tatgcccgcc acctgttcat
     2881 tatcgtgaac ggcgtggtgg ttcttttgtt cctgctggcc tgcgccaacg gcttcctgcc
     2941 gcccggcaaa tggtggctca tcgccctgct cgggttggcc tttccctatc tttttctgtt
     3001 tgtggcgtcc ttctttgccg tcacggtatc gatgccagcc ctgcggcgct ggtcgctgct
     3061 ttcgctcgcg gcactgttga tcggctggtc gaatatccgc catttcatcg cgcttcaccc
     3121 gggcgacagg ttcgttcaac aacgccggcc gggcaccgcg atccgcatcc tcacctggaa
     3181 tgtccgcagc tttgacgact ttgtgaccag gaagcgcccg ccggtccgcc atcgcacggg
     3241 aatgctcgaa tttatcggcg cgcagcaagc cgacgtccta tgtctccagg agttttacga
     3301 ggcgctgacc cggcacggac ctgaatccaa tatcacctat atccaggagc aacttcacta
     3361 tccttactac tatttctccc gcgactatac ccgccccggc ctatacgagg ccggcgtcgt
     3421 catattctcg cgtttcccga tcgtcgacag ttcctgtcac gcttttctga aaccgtatgg
     3481 cttccatacg accgaaagcc tgatctcggc ggatctcgtt gtgggcgccg acaccgtccg
     3541 cgtgtatacg acccatttac aatccgtgct cttcggaagc aaagaattcc gcgaccttca
     3601 gatcatccgg aacgtggacg acagcatcgt ggacgcgtcc cgctccatcg tccgaaaatt
     3661 gcgggagg
//
LOCUS       DATGKE010000579         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_344956, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000579 DATGKE010000000
VERSION     DATGKE010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344956"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /gene="rplD"
                     /locus_tag="VKU83_09860"
     CDS             <1..432
                     /gene="rplD"
                     /locus_tag="VKU83_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLI93906.1"
                     /translation="TGGSRKGNLRNPLYKGGGTIFGPKPRDYDFKLNRKVKDLAKMSA
                     LAYKAKENAIVVVEDLKLEEPKTRQFTDALKGWNIHGKKVLFVMPEENENLYLSLRNL
                     PAVEGTVLADINTYDIVNAEVLVLSETAAKVFSDEAEEAKA"
     gene            460..759
                     /gene="rplW"
                     /locus_tag="VKU83_09865"
     CDS             460..759
                     /gene="rplW"
                     /locus_tag="VKU83_09865"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLI93907.1"
                     /translation="MKLSEVLIKPILTEKANAQQDKLRRYAFRVNRKANKLEIKKAVE
                     DFYGVTVVDVNTTVVPGKNKTRFTKAGYVQGRKPAYKKALVTVSEGETIDLYSSI"
     gene            795..1634
                     /gene="rplB"
                     /locus_tag="VKU83_09870"
     CDS             795..1634
                     /gene="rplB"
                     /locus_tag="VKU83_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLI93908.1"
                     /translation="MALKKYKPITAGTRWRIGNAYAELTADKPEKSLLEKVKSTAGRN
                     VQGRRAMRYMGGGHKKMFRIIDFKRTKKNIDGKVASIEYDPYRTAFIALLNYADGEKR
                     YILAPQGLQVGATVQSGDDVAPELGNALQLRNMPLGTNVHNIEMQPGQGGKLVRSAGT
                     SAQLTNKEDKYAVLKMPSGELRKVLINCYATVGVVSNSDHSLQSMGKAGRNRWRGIRP
                     RNRGVAMNPVDHPMGGGEGKASGGQPRSRNGQYSRGLKTRQKGKGSDKMIIQRKNGKK
                     LAK"
     gene            1652..1927
                     /gene="rpsS"
                     /locus_tag="VKU83_09875"
     CDS             1652..1927
                     /gene="rpsS"
                     /locus_tag="VKU83_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLI93909.1"
                     /translation="MGRSIKKGPYVDANLEEKILAINEGSAKKGVIKTWSRRSTITPD
                     FVGHTLAVHNGNKFIPVYVTEFMVGHKLGEFAPTRNFKGHSSKKIAV"
     gene            1960..2331
                     /gene="rplV"
                     /locus_tag="VKU83_09880"
     CDS             1960..2331
                     /gene="rplV"
                     /locus_tag="VKU83_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLI93910.1"
                     /translation="MEAVAKLRNYPTSPRKMRLLADEIRGMHVERALALLEHHPQHSS
                     TPLHKLLWSAVKNWEQKNEGQSAADAALVVKTVFVDGGRTLKRMRPAPQGRGYRVRKR
                     SNHVTIIVDSTVVTEPLEAAQ"
     gene            2379..>3041
                     /gene="rpsC"
                     /locus_tag="VKU83_09885"
     CDS             2379..>3041
                     /gene="rpsC"
                     /locus_tag="VKU83_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLI93911.1"
                     /translation="MGQKTNPIGARLGIIRGWESNWYGSKKDYASKLIEDNKIRTYLN
                     ARINKGGIAKIVIERTLNKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKEDVQI
                     NILEIRRPELDANIVGDTIARQIENRINYKRAIKMAIASALRMGAEGIKIKVSGRLGG
                     AEIARTEEIKQGRTPLHTLRMDIDYANVYALTVYGKIGIKVWICKGEVLTKRDLNPNF
                     VGG"
BASE COUNT          849 a          683 c          921 g          588 t
ORIGIN      
        1 acgggcggca gccggaaggg caacctgcgc aacccattgt acaagggcgg cggtacgatt
       61 ttcggcccga agccccgcga ttatgatttc aagctgaacc gtaaagtgaa agacctggct
      121 aagatgtcag ccctggctta caaggccaag gaaaatgcga tcgtagttgt cgaggacctg
      181 aaactcgaag agccaaagac ccggcagttt acggatgctc tgaagggttg gaatattcac
      241 ggcaaaaaag tgttgttcgt catgccggag gaaaacgaga atttgtacct gagccttcgc
      301 aatttacctg cggtggaagg gacggtactg gccgatatca acacatatga catcgtgaac
      361 gcggaagtgc tggtgttatc ggagactgcg gcgaaagtat tctcggatga agcggaggaa
      421 gcaaaagcat aatgtaacca ataaaataag taaagagata tgaaactctc tgaagttttg
      481 ataaaaccca tcctgaccga gaaggcaaat gcccagcagg ataagctgcg gcgatatgct
      541 ttccgggtaa accgcaaggc gaacaaactg gagatcaaga aggcggtgga agacttttat
      601 ggggtaacgg tggtcgatgt caatacaacg gtggtacccg ggaagaacaa gacacgcttt
      661 acgaaggccg gatatgtcca gggacggaag ccagcctata agaaggcatt ggtgacggtg
      721 tcggagggag agacgatcga cctgtactcc agcatttagt agaccgaaat tattaaacgt
      781 atacaactag tacaatggca ctgaaaaaat acaaaccgat cacggcaggg acgcgctgga
      841 ggattgggaa tgcctatgcc gagctcacgg ccgacaagcc tgaaaagtcg ctgctcgaga
      901 aagtaaagtc gacggcgggg cgtaatgtcc agggtcgccg ggcgatgcgg tatatgggcg
      961 gcgggcataa gaagatgttc cggatcatcg atttcaagcg gaccaagaag aatatcgacg
     1021 gcaaggtcgc ttccatcgaa tatgatccgt accggacggc tttcatcgcg ttgttgaatt
     1081 atgcggatgg cgaaaagcgc tatatcctgg cgccgcaggg tctgcaggtc ggggctaccg
     1141 tccagtccgg cgatgacgtc gctcctgagc ttgggaatgc gctccagctc aggaatatgc
     1201 ctctcggtac caatgtgcac aatatcgaaa tgcagcccgg tcagggcgga aaactggtgc
     1261 gcagcgccgg tacatccgcg cagctgacca acaaggaaga taaatatgca gtgctgaaaa
     1321 tgccgtcggg agagttgaga aaggtgctga tcaactgtta tgctacggtt ggcgtggttt
     1381 ccaatagtga ccattccctc cagagcatgg gtaaggcggg ccgaaaccgc tggcgcggga
     1441 ttcgtccccg gaaccgcgga gtggcaatga accctgtcga tcatccgatg ggcggtggcg
     1501 agggaaaggc ttccggtggt cagccccgca gcaggaatgg ccagtacagc cgtggcctga
     1561 agacccggca gaagggcaag ggcagtgaca aaatgatcat ccaacggaag aacggtaaaa
     1621 aattagcgaa ataatttaat catctattat catgggtcgt tcaattaaaa aaggtccgta
     1681 tgtagacgct aacctcgaag agaagatcct tgcgataaac gagggatctg ccaagaaagg
     1741 ggttatcaag acttggagtc gtcgcagcac catcacgccg gatttcgtag gtcataccct
     1801 ggccgtgcac aacggcaata aattcatccc ggtgtatgtc acggagttca tggtggggca
     1861 caagctgggc gagtttgcac ctacccggaa ttttaaaggg cattcaagta aaaagatagc
     1921 agtataaccc gcatgagcgg catgcaaaaa acgatactaa tggaagcagt agccaaattg
     1981 agaaattacc cgacatcacc ccgtaaaatg cgtttactgg ctgatgagat ccgcgggatg
     2041 cacgtagaaa gggccctggc cttgctggag catcatccgc agcattcatc gacgccgctg
     2101 cataagttgt tgtggtcggc cgtcaagaac tgggagcaga agaatgaggg gcagagcgcc
     2161 gcagatgcag ctttggtggt aaagacggta tttgtcgatg gcggcagaac gctgaagcgg
     2221 atgcgtccgg cgccgcaggg ccggggatac cgggtccgga agcggagcaa tcacgtcacc
     2281 atcattgttg attcaacggt ggtcaccgaa ccgctggagg cggctcagta ataggtcaga
     2341 ggtaaaaacc aaatgattaa acttatttaa ttaaaaatat gggtcagaaa acgaatccta
     2401 ttggggccag gttagggatc attcgcggtt gggaatccaa ctggtatggc agcaagaaag
     2461 actatgcttc caagctgatc gaagacaaca agatcaggac ttacctgaat gcccggatca
     2521 ataagggtgg gatcgccaag atcgttatcg agcggacgct gaacaagctc atcgtgacga
     2581 tccacacttc caagccgggg atcatcatcg gcaagggcgg cggcgaggtg gacaggatta
     2641 aggaagagtt gaagaagctg acggggaagg aagatgtcca gatcaatatt ttggaaatcc
     2701 gccggccgga gctggatgcg aatatcgtgg gtgacacgat agcgcgccag atcgagaacc
     2761 gcatcaatta caagcgggcg atcaagatgg cgatcgcttc ggcgctccgc atgggtgccg
     2821 agggtatcaa gataaaggta tcgggtcggt tgggcggagc tgaaatcgcc cgtacggagg
     2881 agatcaagca aggcaggacg cctttgcata cgctccggat ggatatcgac tatgcgaacg
     2941 tttatgcgct taccgtttac gggaagatcg gcatcaaggt gtggatctgt aagggcgagg
     3001 tgctgacgaa gcgcgacctg aaccccaatt ttgtcggtgg a
//
LOCUS       DATGKE010000580         5680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1483780, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000580 DATGKE010000000
VERSION     DATGKE010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483780"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VKU83_09890"
     CDS             complement(<1..376)
                     /locus_tag="VKU83_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93912.1"
                     /translation="MRRCIPVLALGFFLLSGCKAKKPSSNLSFTVDTAELKAMDSMTA
                     AERRNDSALLKSATQTPGYNAGLDSITFPTPAGWRRYDTALGSIRVLMLDTASSSPRF
                     RINVNIVSDSLRGASIDAYTKGT"
     gene            462..950
                     /locus_tag="VKU83_09895"
     CDS             462..950
                     /locus_tag="VKU83_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLI93913.1"
                     /translation="MDKKHQDLFIRTVLANWNLAIGRITSVFDRQSDADLLQPIAPGK
                     NRVVYLLGHLTAVHDRMLPLLGFGERRFPHLDELFITNPDNPQAAIPAVAQLRGQWAA
                     INQLLTEKLSALTPEEWLAKHESVSAADFEKEPHRNRLNVVLSRTGHVSYHHGQLALL
                     KL"
     gene            997..1806
                     /locus_tag="VKU83_09900"
     CDS             997..1806
                     /locus_tag="VKU83_09900"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI93914.1"
                     /translation="MSFFQDKVVVITGGSDGIGKALIETLIPLGAKVATCGRSHDKLY
                     TLQMEYSQVLLHAVACDVSNEWECKKFIESTLETFGRIDVLINNAGISMRALFLDCDT
                     EVTRKVMEINFLGAVYCTKYALPSLLATNGTVVGISSTAGYRGLPGRSAYSASKFALQ
                     GWLESLRTELLHSGVNVMWVCPGFTASNIRVAALNKDGQASGESVLNEGSLMTAAECA
                     RHIVRAIARRRRTVVLTFLGKVTVLINKLAPALADRLTYRHYYNRKGEFVK"
     gene            1812..2633
                     /locus_tag="VKU83_09905"
     CDS             1812..2633
                     /locus_tag="VKU83_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLI93915.1"
                     /translation="MALITLTSDIGHKDYLVGAVKGQLVQVNPGFQIVDISHELSPFN
                     YPQAAYICRNAIRQFPPFTYHLILVNLFERRPEQLLLAFHKEQYLLCADNGLLTMILE
                     GRPEMVIGLPLERTAVKNTLYCVKVMAQAIQGLVTGGGLLSIGTPDVPFTEKNHLRPV
                     FGDNWIEGQIIFIDHFENVIVNITREEFEERRKGRSFRIVFKRDEVIERISETYADVG
                     EGEKLALFNSAGYLEIAIQKGNAAGLFGLQDFTEQSQNQYLQSRLFYQTVRVYFE"
     gene            2704..>5680
                     /locus_tag="VKU83_09910"
     CDS             2704..>5680
                     /locus_tag="VKU83_09910"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HLI93916.1"
                     /translation="MKFNRVNTIVGWIVGLTACVVFVMTAEATGSFWDCGEFISSCYK
                     IQIPHPPGAPLFVLMGRFFIILFGDSPHTAAHAVNFMSAIASGLSILFLFWSITHFAR
                     RIVQKSDYTVEPDRRQVFTIMACGVVGALAYTFTDSFWYSAVEGEVYASSAFFTALVF
                     WAILKWEQQADRPGADRWIIFIFYMMGLSIGVHLLNLLTIPAVVMVYYFRRYRPTTWG
                     AIWAFVVGCALTGFVQKFVIQYTIKGAADFDVFFVNSLNMPFFVGFGFFFVLLAVVLV
                     LINRWAVRKKYFYLKIACWSFGFMLLGYSTYFTTMIRSNADPGVDMYNVDNPVSLEGY
                     LSREQYGDWPILYGPDFEDTPPRVDAGPLYVKGKDKYEVAGRLTSEDWGATPSAHLFP
                     RMWDNGNDRQQLNCYQAYTGLTDQEVPTMADNIKFFIGYQNWEMFLRYFFWNYSGKQN
                     DLQGFGNVRDGNAISGISVIDNALYGDQSRMPDSIHKDNKSYNRMYLLPIILGVIGLL
                     LQSSRNRRDFLVTLLLFFFTGFAIVIYLNQSGYQPRERDYAFAGACYAFAIWIGLGVI
                     KVQEWIGKFSFKARADLAGYAAAGLCFLAVPVLMGSQEWNDHDRSHKTLPRDIGKDYL
                     ESCPKNAILFSFGDNDTYPLWYAQEVEGIRPDVRVMNYSLLGTDWYINELRYKVNESA
                     PFDVLFTPEQIQGSTRDAVPVSPLPGFDQKKYYDAYDMFRNVVGSDDPKYQLGSQDGT
                     TTFSVLPVRHLSVPVDTNRVRTNGTVNAGDSVVSELHLDISPDKNYLFKNELAVLAII
                     AANKWQRPICFNSTYEVEALGLARYIRQNGLAGQLVPVEPRDNAYGFYNNDFAYQVMM
                     TKFGYGNANIPGVYYDEENRRHLNTIRAAHAQLALSLVDAGKKDSARNVLEHFDQNVL
                     ESNFPYGMTSTFGNQQNKISMSFLLACYRSNDMKLAAKVAASLRKDLEQQLRYYNSLG
                     ESQPNEQLAINAQLAKQNKG"
BASE COUNT         1273 a         1548 c         1584 g         1275 t
ORIGIN      
        1 cggtgccctt ggtataagcg tcgatcgacg ccccgcggag cgaatcgctc acgatattga
       61 cgttgatcct gaaccgcggg ctgctggaag cggtgtccag catcagcacc cggatactgc
      121 cgagcgcggt gtcatagcgc cgccatccgg ccggggttgg aaaggtaatg ctgtccagtc
      181 cggcgttata gcccggcgtt tgggtggctg atttgaggag cgcggaatcg ttccggcgct
      241 ccgcagccgt catcgaatcc atggccttga gctccgcggt gtcgaccgtg aatgacaagt
      301 tgctggaagg cttcttagcc ttacagccgg acaacaggaa aaatccaagc gccagaaccg
      361 gaatgcatcg tctcatcctg aaaaaattat ggcaaaatta gttcaattgc cgtgctcaac
      421 aaatactcat cccaggttgt taaccggctt aaacatagga aatggacaag aaacatcaag
      481 acttattcat caggaccgtc ctcgctaact ggaacctcgc gattggccgg atcacaagcg
      541 ttttcgaccg ccaaagtgac gcagacctgc ttcaaccgat tgcgccgggg aagaaccgtg
      601 tcgtgtacct tctcgggcat cttaccgccg tgcatgaccg gatgctgccc ctcctgggct
      661 tcggcgaacg gcgttttccg cacctcgatg aactattcat caccaacccg gacaatccgc
      721 aagctgcgat ccccgccgtc gcgcagctgc gcggtcaatg ggccgcgatc aaccagctgt
      781 tgacggaaaa actcagcgcc ctgacgccgg aggaatggct ggcaaagcat gagtcggttt
      841 ctgccgcgga ctttgaaaag gaaccgcacc ggaacaggct gaacgtggtg ctgagcagga
      901 caggtcatgt cagctatcat catggccagc tcgccttgtt gaagttgtag gaacgaggga
      961 agtcccttac ttttacaacc acaacaaaac ccttacatgt catttttcca ggataaagtc
     1021 gtggtgatca ctggtggcag cgacggaatc ggaaaagcgc tgatcgaaac ccttatcccc
     1081 ctgggcgcga aggtggccac ctgcggccgc agtcacgata agctgtatac gttgcaaatg
     1141 gaatattcgc aggttctgct ccatgccgtc gcctgcgacg taagcaatga gtgggaatgc
     1201 aaaaagttca tagaatcgac tctcgagacc tttggccgca tagacgttct catcaacaat
     1261 gcgggcatat cgatgcgggc gctgttcctc gactgtgata ccgaggtgac ccggaaagtt
     1321 atggaaatca acttcctcgg tgcggtttac tgcacgaaat atgcgcttcc ttcgctgctc
     1381 gcaactaatg gcacggtggt ggggatttcc tccacggcgg gctaccgtgg cctgcccgga
     1441 agatcagctt attccgccag caaatttgca ttgcagggat ggctggagtc gttgcgaaca
     1501 gaactcctgc acagcggcgt caacgttatg tgggtatgcc ccgggttcac ggcttcgaat
     1561 atccgggtgg cggcgctgaa caaggatggc caggcgagcg gcgaatctgt tttgaacgaa
     1621 ggttcgctca tgacagcggc ggaatgtgcc cggcacatcg tccgggctat agccaggcgg
     1681 cgccggacgg ttgtgctgac ctttctggga aaagtgaccg tcctgatcaa caaactggcg
     1741 ccggccctgg cggaccggct gacctaccgg cattattaca atcgaaaagg ggagtttgtc
     1801 aaataaccgc aatggcccta atcaccctca catcggatat cggccataaa gactatctgg
     1861 tcggggcggt caaaggtcaa ttagttcagg tgaaccccgg tttccagatc gtggacatca
     1921 gtcacgagct ctcccccttc aactacccgc aggccgccta tatctgccgc aacgccattc
     1981 gccagttccc gccgttcacc tatcatttga tccttgtaaa cctgttcgag cggaggccgg
     2041 aacaactgtt gctggcgttt cataaagagc aatacctcct ttgcgcggac aatggcctgc
     2101 tcacgatgat cctcgaggga cgaccggaaa tggtgatcgg gctgccgttg gaaaggaccg
     2161 ctgtcaagaa tacgctctat tgtgtgaaag tcatggccca ggcgatccag gggctggtga
     2221 ccgggggcgg cctcctgagt atcggcacgc ccgacgtgcc gttcaccgaa aagaaccacc
     2281 ttcgcccggt attcggtgac aactggatcg aaggccagat catcttcatc gatcatttcg
     2341 aaaatgtcat cgttaatatc acgcgcgagg aatttgaaga acgccggaaa gggcgcagct
     2401 tcaggatcgt attcaaacgc gacgaggtga tcgagcggat cagcgaaacc tacgccgacg
     2461 tcggcgaggg cgaaaagctg gccctcttta attccgcagg ctatctcgaa atcgccatcc
     2521 agaaagggaa tgccgccggg cttttcggct tgcaggactt taccgagcag tcgcagaacc
     2581 agtatctcca aagcaggctt ttctatcaga ccgtcagggt gtattttgaa tagttaacac
     2641 ggcgaatccg ggaaattcgc cattttatct gttaattcgc gttccaaatt gatgtcgaat
     2701 accatgaagt tcaacagagt gaacactatc gtaggctgga tcgtcggact cacggcctgc
     2761 gtcgtatttg tgatgaccgc agaagctaca ggcagtttct gggattgcgg cgagttcata
     2821 tccagctgtt acaagatcca gatcccgcat cctcccggcg cgcctttatt cgttctgatg
     2881 ggccggttct ttattatttt gtttggcgat agccctcaca ccgccgcgca cgctgtcaat
     2941 tttatgagtg cgatcgcgag cggattgtcc atcctgttcc tgttctggtc gataacccat
     3001 ttcgcccggc gcatcgtaca aaagtccgac tacacggtag aaccggatcg ccgccaggtc
     3061 tttacgatca tggcttgcgg cgttgtcggc gccctcgcct acaccttcac cgactccttc
     3121 tggtacagcg cggtggaggg tgaggtctac gcctcctcgg ccttttttac cgccctggtc
     3181 ttttgggcga tcctcaaatg ggagcagcag gcggaccggc ccggggccga ccgttggatc
     3241 attttcatct tttatatgat ggggctctcg atcggcgtgc acctcctcaa tctgctgacg
     3301 attcccgccg tggtaatggt gtattatttc cggcgctacc ggccgacgac atggggcgcg
     3361 atctgggcat tcgtggtcgg ctgcgccttg acgggcttcg tgcaaaaatt cgttatccag
     3421 tataccatca aaggcgcggc ggattttgac gtcttcttcg tgaacagcct gaacatgccg
     3481 ttctttgttg gttttggttt cttttttgtt ctccttgcgg tggtacttgt tttgatcaac
     3541 cgctgggcgg tccggaaaaa atatttctac ctgaagattg cctgctggtc gttcggattc
     3601 atgctccttg gctattcgac ttatttcacg acgatgatcc gcagcaatgc cgatccgggg
     3661 gtggacatgt ataacgtcga caatcctgtg agcctggaag gctaccttag ccgcgagcaa
     3721 tacggcgact ggccgatcct gtatgggccc gacttcgaag acacgccgcc gcgggtggat
     3781 gccggcccgc tctatgtaaa gggaaaggac aaatacgagg ttgccggaag gctgacgtcg
     3841 gaagactggg gcgccacgcc gtccgcgcat ctctttccgc ggatgtggga taatggcaac
     3901 gaccgccagc agctcaattg ttaccaggca tacacgggcc tgaccgacca ggaggtgccg
     3961 accatggcgg acaatatcaa attcttcatc ggctaccaga actgggaaat gttccttcgg
     4021 tattttttct ggaattattc cggaaagcag aacgacctcc agggtttcgg gaatgtccgc
     4081 gacggcaatg ccatttccgg gatatccgtt atcgacaacg ccttgtatgg agatcaaagc
     4141 cggatgccgg acagcatcca caaggacaac aaatcctata accggatgta tttgctgccc
     4201 attatcctgg gagtgatcgg acttctcctt caatccagcc gcaaccggcg ggatttcctg
     4261 gttacgctat tgcttttctt ctttacgggc ttcgccatag ttatctacct caatcagtcc
     4321 ggctatcagc cgcgggaacg ggattatgcc tttgccggcg cctgttacgc gtttgcgatc
     4381 tggatcgggc tgggtgtcat caaagtgcag gaatggatcg ggaaattttc gttcaaggcc
     4441 cgggccgatc tggccggcta tgccgccgcg gggctatgtt tcctggcagt gccggtgctc
     4501 atgggcagcc aggagtggaa tgaccatgat cggagccata agacccttcc cagggatatc
     4561 ggcaaggatt acctcgaaag ctgccctaaa aatgccatcc tgttttcttt tggcgacaac
     4621 gacacctatc cgctctggta cgcgcaggaa gtcgagggca tccggccgga tgtccgggtt
     4681 atgaattaca gcctgctggg tacggactgg tatatcaatg agttacgata taaggtcaat
     4741 gaaagcgccc cctttgacgt actcttcacc ccggaacaga tccagggcag tacccgcgat
     4801 gcggtaccgg tgagccccct gcccgggttt gaccagaaga aatattacga cgcgtacgac
     4861 atgttcagga acgtcgtcgg cagcgacgac ccgaaatacc agctgggaag ccaggacggc
     4921 accacgacat tcagtgtcct gccggtaagg catctatcgg tgccggtgga cacgaaccgg
     4981 gtaaggacga acggaacggt gaacgccggc gatagcgttg tcagcgaact gcacctggac
     5041 atcagtccgg acaaaaacta tttgttcaag aatgagctcg ccgtcctggc aatcattgcc
     5101 gccaataaat ggcagcgccc catctgcttt aattcgacct atgaggtgga ggccctggga
     5161 cttgccaggt acatccggca gaatgggttg gcggggcagc tggtgccggt ggaacccagg
     5221 gataacgcct atggattcta caataatgat tttgcgtacc aggtcatgat gacaaagttt
     5281 gggtatggaa acgccaatat ccccggcgtt tactatgacg aagagaaccg gcggcatctg
     5341 aataccatcc gggcggcgca cgctcagctg gcgcttagcc tcgtcgacgc aggcaaaaaa
     5401 gattccgcca ggaacgtgtt ggagcatttt gaccaaaatg tgctggagtc gaatttcccc
     5461 tatggaatga cctcgacctt tggcaaccag caaaataaga tctccatgag ctttttgctc
     5521 gcctgttacc ggtccaacga catgaaactg gcggccaagg tcgcggcgtc gctccggaag
     5581 gatctggagc aacagttgcg atattacaat tccctcggtg aaagtcagcc gaacgaacag
     5641 ttggcgatca acgcacagct ggcgaagcag aataagggcg
//
LOCUS       DATGKE010000581         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_42395, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000581 DATGKE010000000
VERSION     DATGKE010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42395"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VKU83_09915"
     CDS             complement(<1..611)
                     /locus_tag="VKU83_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93917.1"
                     /translation="MKKSLILSSLSCCCFFFSFTVTAQNEWPRTLMAPDGAILTLYQP
                     QPDSFRNNILQYRSAFSLLEKGKTEPEFGMFQAIATVETDRDDRLVTLLSVKIPILKL
                     PDNPDAARINQLKATLEAGIPGLDLAIPLDELLTALDMNTGEKKLSLGLNNQAPRIIF
                     ATQPSILVIIDGEPRTGMNKDLGMEVVVNTPYTILRRGKGWYL"
     gene            779..1636
                     /locus_tag="VKU83_09920"
     CDS             779..1636
                     /locus_tag="VKU83_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI93918.1"
                     /translation="MQTFLIATDFSAASRNATQYGIELAKAFNGRVVLVSAYQQVPVT
                     LTDSLVLATPADQGEVIRQQLELEADFFHSEEATPLEIIAREGPSSNVILEAAKELNA
                     DLILAGMKGSGKATRRFFGSTVTSVARKTTVPLMVIPETVTYSRPGTIALANDISFHA
                     GTHLLDFLRMLVERFHSRLYIVRIVTKKSNEVVEVLNRPSNLTKMVSKLEPLYEYPLD
                     KNITKALKHFISTHHVDLLAMVPHREFLPERWFLRSNTREMIFKTDIPLLILPELTHR
                     GAHQHSVVF"
     gene            complement(1679..>2413)
                     /gene="ligD"
                     /locus_tag="VKU83_09925"
     CDS             complement(1679..>2413)
                     /gene="ligD"
                     /locus_tag="VKU83_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HLI93919.1"
                     /translation="IFTALQQIRTPMVIDGEIVALDKEGRSGFQLLQEYEENPDIPLR
                     YYVFDILEHNGKDVTGLPLLERKALLKKILPEGDVIRYCHHVEADGLKFFNDVRKKNL
                     EGMLAKRKDSPYLPGKRTSLWLKVKNQHTEEVVIVGFTAPRGSRKRFGALVLGIYKKG
                     KLVYAGHTGTGFTDQLLDDLYDKMLPLIHGQSPFDTEIKTANGVTWLRPKLVANVRFT
                     EWTKDGIMRHPVFQGLRIDKTVEELK"
BASE COUNT          612 a          590 c          559 g          652 t
ORIGIN      
        1 tacaggtacc aacctttacc gcgccgcaag atagtataag gtgtattcac tacgacctcc
       61 atgcccagat ccttgttcat tcctgtccgg ggctctccgt ctatgatcac caatatagaa
      121 ggttgggttg caaagattat ccggggcgcc tggttgttta gtcccaggga cagtttcttt
      181 tcccctgtgt tcatatccag cgcggtgagc agctcatcca ggggaatagc cagatccaat
      241 cccggaatgc ccgcttccag ggttgctttg agttggttga tcctggccgc atccgggtta
      301 tcggggagct tcaggatagg gatttttacc gacagaaggg tcaccaggcg gtcgtccctg
      361 tcggtctcca cggttgcgat agcctggaac atgccaaatt cgggttctgt ctttcctttt
      421 tccaaaaggg aaaaggccga acggtattgc aaaatattgt tccggaacga atccggttgc
      481 ggttggtaaa gggtcaggat ggcgccatca ggcgccatca gcgtccgggg ccattcattt
      541 tgcgctgtta cagtaaaaga aaaaaagaag cagcaacagg acaaggagga tagaatcaat
      601 gactttttca taatataaaa accggcgctt tccgataaaa accggcgctt tccgcctgtt
      661 aaagctattc cctaatcctc cttccagaaa tgatatccat cagtttcatt gctgagtagt
      721 atcaccccca aacaaataat tgggttgtaa cttttgctta agctaaataa tgtcccaaat
      781 gcagactttt ctgatagcaa cggatttttc agccgcatcg cggaatgcca ctcaatatgg
      841 aatagaactg gccaaggcat tcaatggaag agtagtcctg gttagcgctt atcagcaagt
      901 acccgtcacg ctcaccgatt cattggtgtt ggcaacgccg gcggatcagg gcgaggtgat
      961 tagacagcaa ttggaattgg aagcggactt ttttcattcc gaagaagcta caccccttga
     1021 aataatcgcc agggaaggtc cctcgtccaa tgtgatcctc gaagctgcca aagagctgaa
     1081 tgcggacctt attttggccg gcatgaaagg aagcggcaaa gcaacgcgca ggttttttgg
     1141 cagtacagtc acgtcagtag caagaaaaac gactgttccg ttaatggtaa ttccggaaac
     1201 ggtgacctat tcaaggcctg gaaccatcgc cctggcaaac gatatttcct ttcatgcggg
     1261 cacgcactta cttgatttcc tacgcatgtt ggtggaaagg tttcattcga gactatatat
     1321 agtgagaata gtcacgaaaa aatcaaacga agtggtcgaa gtcctgaacc ggccctccaa
     1381 cctaaccaaa atggtatcga aactagagcc cctttatgaa tatccgctgg ataagaacat
     1441 aacgaaagcc ctgaagcatt ttatttctac ccatcatgtc gatctgctgg cgatggtgcc
     1501 ccatcgcgag ttccttcctg aaagatggtt cctccggagc aataccaggg aaatgatctt
     1561 caaaacggat attcctttgt tgatattgcc tgagttaact catcggggag cgcaccagca
     1621 ttcggtcgtt ttttagcttg ggaaattgct tttgttcctt ttgcatgtag ctatcatttt
     1681 attttaattc ctcaacggtc ttgtcgattc tcaaaccctg gaaaacggga tgacgcatga
     1741 tcccatcctt tgtccattcc gtaaagcgga cattggcgac gagctttggt cttaaccatg
     1801 taacaccatt agccgttttt atttcagtat cgaaaggcga ctgcccgtgt atcaggggaa
     1861 gcatcttatc atacaagtcg tccagtaatt gatcggtaaa tcctgttccc gtatgcccgg
     1921 cataaacgag ctttcccttt ttgtagatac cgagcaccag tgcgccgaac cttttccggg
     1981 aaccgcgtgg cgctgtgaag ccaacgatca ccacttcttc cgtatgctgg tttttcacct
     2041 taagccagag cgaagttctt tttcctggca ggtaggggct atctttccgt ttggccagca
     2101 taccttccag gttcttcttc cggacatcat tgaagaattt caggccgtcc gcttcgacat
     2161 gatgacaata acggataaca tcgccttcag gtaatatttt tttgagcaag gctttccttt
     2221 ccaacagggg aagcccggta acgtctttac cattgtgctc caggatgtca aaaacataat
     2281 agcgcagggg aatatccggg ttttcctcat attcctgcaa taactgaaat ccggatctgc
     2341 cctctttatc cagcgctaca atttcgccat cgatcaccat tggggtcctg atctgttgta
     2401 atgccgtaaa aat
//
LOCUS       DATGKE010000582         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1265977, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000582 DATGKE010000000
VERSION     DATGKE010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265977"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VKU83_09930"
     CDS             <1..192
                     /locus_tag="VKU83_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93920.1"
                     /translation="IGISDSMTVQDLEQAMAAYFNAPVLVCRKSGRNWIETRMTRRWT
                     LKQQNDLGRDLSTPDKRVE"
     gene            complement(229..1287)
                     /locus_tag="VKU83_09935"
     CDS             complement(229..1287)
                     /locus_tag="VKU83_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI93921.1"
                     /translation="MRKILVIDDHDDIRENIAEILTLAGYEATTAPNGKKGVEAALRD
                     RPDLVICDIMMPELDGYGVLHLLRKNEATENIPFIFLTARTERADFRKGMEMGADDYI
                     TKPFDDIELLNAIEIRLKKYDLLQTKYPAGEKGASEMIHDLTESGLLKLDPDNYEFEE
                     VSKKLSLYMEGRRPKYLYYLKKGKIKAYRVHEDGKEYITNLYTAGDYIGYLPLLENAV
                     YSDSAEALEDSELAQIPKDEFLSAVYHDIQIATRFIRLIAQNVQEKEERLLSLAYGSL
                     RKRVAAALVDIDAKFNKDSSNGRSLDISREDIAQYVGTATESLIRTLSDFKSEKLIEI
                     KEGKIRITDLSRLKNLLY"
     gene            1427..>2937
                     /locus_tag="VKU83_09940"
     CDS             1427..>2937
                     /locus_tag="VKU83_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HLI93922.1"
                     /translation="MPTVTTQEDRCEHCGTACGPGSVRLDDKAFCCEGCKMVYSLLSG
                     HGLGGYYTYNQRPGVNRRAPLRKDKYAFLDDEQVSQRLYAFRSDQECHIAFQLPQIHC
                     SSCLYLLENLRRLDRAILSSRVDFGRRQAFIVFDPAMTSLRKVAELLALVGYEPYISL
                     GDLGGKRPGMDRRRIYQIGIAGFCFGNIMLLSFPEYLGLGASETMFRNAFRWLSLGLS
                     LPVAAFAAQPFYASAWKGIRHRFLNIDAPIVLALVVTFVRSAYDVLSGNGSGYFDSLA
                     GIVFFMLAGRILQERTHRRLAFDRDYTAYFPIAVTVTRGETTMTKMLPEIKYGDTMLI
                     HSGELVPADGIVTKGAASIDYSFVTGESLPVSKETGEIVYAGGRQTGGMIEVLATREV
                     AQSYLTQLWEREEFRQNDPAEKRNSFTNGLSRSFTYIVLSIAISAGVYWSLNDPGRTW
                     AAVTSVLIIACPCALLLSATFTNGNILRILARNQFYLRHAQVIERVAAANRIV"
BASE COUNT          583 a          838 c          816 g          700 t
ORIGIN      
        1 atcggaattt ccgattccat gaccgtccag gacctggaac aggcaatggc cgcttatttc
       61 aatgcgcccg tcctggtctg ccggaaaagc ggcaggaact ggatcgaaac ccgaatgacc
      121 cgcaggtgga cgctgaaaca acagaatgat ctgggtcgcg acctttcgac cccggataag
      181 cgggtcgagt aggcccggaa gagcgagccg gccaggcaac gccttcggct aatataacag
      241 gtttttcagc cggcttagat cggttatccg gatcttccct tccttgattt cgatcagctt
      301 ctccgacttg aagtcgctca aagtgcggat cagcgactca gtggccgttc cgacgtactg
      361 cgcaatatcc tcccgggaga tgtcaaggct ccttccgttg ctgctatcct tgttgaattt
      421 agcatcgatg tccaccagcg cggcggccac gcgcttgcgg agcgagccat aagcgaggct
      481 gagaaggcgt tcctcctttt cctgcacgtt ttgcgcgatc agccggataa agcgcgtcgc
      541 gatctggata tcatggtaca cggccgaaag gaactcgtcc ttggggatct gagccagttc
      601 ggaatcctcc agcgcctcgg cagaatcgct gtagaccgca ttttcaagca gcggcaggta
      661 gccgatatag tcacccgcgg tgtacaggtt ggtaatatat tccttcccgt cttcatgcac
      721 gcggtacgct tttattttcc ctttcttcag gtagtacagg tatttcggcc gcctgccttc
      781 catgtatagc gacagcttct tggagacctc ctcgaattca tagttatcgg gatcgagctt
      841 cagaagcccc gattccgtca gatcatggat catttcgctg gcgccttttt ctcctgccgg
      901 gtatttggtt tgcagaagat cgtatttttt cagccggatc tcgatcgcgt tgagcagctc
      961 gatatcgtcg aagggtttgg tgatatagtc gtcggcgccc atctccattc ccttgcggaa
     1021 gtcggctctc tctgtccggg cggtcaggaa gataaatgga atattttccg tcgcctcgtt
     1081 ctttcgcaac aaatgcaaca ccccataacc atccagttca ggcatcatga tatcacagat
     1141 caccaggtcg gggcggtcgc ggagagcggc ctccacgcct ttctttccat tgggtgcggt
     1201 tgtcgcctcg tatccagcca aagtcaggat ctcagcaatg ttctcccgga tatcgtcgtg
     1261 atcatcgatc accagtattt tgcgcatggt gccttgtttt ggggtgacgt aaaagcccta
     1321 caatactaca acttttggtc aatatcccgg tgaccaggat cagccgcctt tttgacgaag
     1381 atcatctccg gcgggcggga cgcccggtag tttagcgctt tgctccatgc ctacggtcac
     1441 gacacaggaa gacaggtgcg aacattgcgg aaccgcttgc gggccgggtt ccgtgcggct
     1501 tgacgacaag gctttctgct gcgaaggctg caagatggtc tattccttgc tgagcgggca
     1561 tgggctgggc ggctactaca cttacaatca acggccaggc gtcaatcgcc gggcgcccct
     1621 gcgaaaggac aaatacgcgt tcctggacga tgagcaggtt tcgcagcgct tgtatgcctt
     1681 cagaagcgat caggaatgcc acatcgcgtt ccagttaccc caaatccatt gcagctcctg
     1741 cctttatctt ttggaaaatc tgcgccgcct cgaccgggcc atcctttcgt cccgggtgga
     1801 ctttggccgg cggcaggcgt ttattgtttt tgatccggcc atgaccagcc tgcggaaggt
     1861 cgccgaattg ctcgccctcg tcggctacga accttatatc agtctcgggg acctgggtgg
     1921 taaacgtccg ggcatggacc ggagaaggat ctaccaaata gggatagccg ggttttgttt
     1981 tgggaatatc atgctgctga gcttccccga atacctgggg ctcggagcaa gcgaaaccat
     2041 gttccgaaat gcattccgct ggctcagcct gggcctgtcc ctgccggtgg cggccttcgc
     2101 cgcccaaccc ttctatgcgt cggcctggaa aggaatccgg caccggttcc tgaacatcga
     2161 cgcgccgatc gtcctggccc tcgtcgtgac ctttgtccgc agcgcttatg acgtgctatc
     2221 cgggaacgga agcggttatt tcgattcgct cgctggcatt gttttcttca tgctcgccgg
     2281 gcggatcctt caggagcgta cgcaccggcg cctggctttt gacagggact atacggccta
     2341 tttcccgatt gcggtcaccg taacgagggg agaaaccacc atgacaaaaa tgttgccgga
     2401 gatcaaatat ggcgatacga tgctgatcca ctcgggcgag ttggtccctg cggacgggat
     2461 agtgaccaaa ggcgcggcgt cgatcgatta cagttttgtg accggcgagt ccctgccggt
     2521 gtcaaaggag accggtgaga tcgtctatgc gggcggccgc caaacgggcg ggatgatcga
     2581 ggtgctcgcg accagggaag tggcgcaaag ctatcttacg caattgtggg agcgcgagga
     2641 attccggcaa aacgacccgg cggaaaaaag gaactccttt accaacggcc ttagcaggtc
     2701 ctttacctat atcgttcttt ccatcgccat ctcagcgggc gtttattgga gcctgaacga
     2761 ccctggacgc acctgggctg cggtcacctc cgtactgatc atcgcttgcc cttgcgccct
     2821 gctcttgtca gcgaccttta ccaacggcaa tatactccgc atccttgcgc ggaatcaatt
     2881 ctatctgcgc catgcgcagg tgatcgagcg cgtggccgct gccaaccgta tcgtctt
//
LOCUS       DATGKE010000583         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_674257, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000583 DATGKE010000000
VERSION     DATGKE010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674257"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VKU83_09945"
     CDS             <1..314
                     /locus_tag="VKU83_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HLI93923.1"
                     /translation="EILFIKNLTREMTPEEQMRFITIYQGRRKDQQTILILTCLGFVG
                     VSGIQRFVLGEIGMGVLYLFTGGICMIGTIVDLINSQKLVNEYNQKQAFEAMNIMRAI
                     S"
     gene            348..418
                     /locus_tag="VKU83_09950"
     tRNA            348..418
                     /locus_tag="VKU83_09950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Gln,seq:ctg)
     gene            768..1718
                     /locus_tag="VKU83_09955"
     CDS             768..1718
                     /locus_tag="VKU83_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93924.1"
                     /translation="MIKNYFLVAVRHFLRHRLFALINVFCLAIGLTFSMLIGVYILQQ
                     RSVNTSLRNIDRQYVIKSIWKEKTMGMEQTSIGPLAETLHKSYPGLVANYFRYNPVTN
                     VVSAGDRYFKENIAICDTTLISMYGFPLLYGDATNPFPTDNSAVITEAMAKKLFGNDN
                     PIGKAINILTIGQGNQGYTVSAVLKSMPYNSVNNLIDKYGYSVFVPFAGSHYYSMAGG
                     AGQDDWTQIFMVSMVELKPGVAAGDLEAPAKKILALHLAKNIKGFLQPAFAPLKDYYL
                     NDNNGAVRQMITTLSIIAVSFWLWRSSILSTSISALPPIA"
     gene            1664..2788
                     /locus_tag="VKU83_09960"
     CDS             1664..2788
                     /locus_tag="VKU83_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI93925.1"
                     /translation="MAIINFVNINIGTSSYRVKEIGLRKVFGGARKQLIGQHLTESIT
                     LTVVAGLISLGLYEITRPYFSQVLHVPMDSLFAFGGFQYLLLATLIIVVGCLSGIYPA
                     FVLSSTDIIPSIKGKIDTGRGSLALRKALLITQFTLAVTVFISALNVSKQVSYIFNRD
                     LGYNREQLVVLTVFPKQWDSAGIQHMVTVRNELANTPHVHDASVSFEVPERTPPNSLA
                     IVRTGSDQTVQVPAMVADENFGGTFGMHLVKGNFFDDYRRGAGKDEIVLNETAAKAAG
                     ITEPNQAVRTSQGFTYKVSGIVKDYNYSSFEQRIGPLAILHMKDNTSYRYLTLKIDGR
                     DAPATLAAVGKSGESWRLAHPSITISWIHDSGPCISRKCN"
     gene            2743..3168
                     /locus_tag="VKU83_09965"
     CDS             2743..3168
                     /locus_tag="VKU83_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI93926.1"
                     /translation="MDTRFRSLYQSEVQLKQATNMATILNLVIVFLGIFGMVAFSLAR
                     RIKEISIRKILGAEAKNIIALFLKEYAILILIANMIGWPLAYLATDHWLSNYSYRMQQ
                     DIVPYASVLVIVFMSAFVFISALCFRVAIANPVRNLKME"
BASE COUNT          830 a          813 c          848 g          851 t
ORIGIN      
        1 aagaaatcct tttcatcaag aatttgacca gggaaatgac gccggaagag caaatgcgtt
       61 ttatcacgat ttaccaggga cgaagaaaag atcagcaaac gatattgatt ttgacttgtt
      121 taggtttcgt gggcgtctca ggcatccaga gatttgtcct tggcgaaata gggatgggtg
      181 ttctttattt atttaccggc gggatttgca tgatcggaac cattgtggac ctgatcaatt
      241 cgcaaaagct ggtcaatgaa tacaatcaga agcaggcgtt tgaggccatg aacattatgc
      301 gtgcgattag ttaaacttcc gaaaaaatct tagttttgcg gtcccattgc ccaatagtat
      361 aagggtagta cgtcagattc tggatctgag tgtcttggtt cgaatccagg ttgggcaaca
      421 acgagccccg cgctatgcgc ggggttttgt tttatccggg agcgtgcaga gcttgctctg
      481 ctaagcggac ggtaaaacaa aactagcccg gcctgagccg ggcgtggctc gttgggtaat
      541 gcaaatgcgg ggtggatcag cggaggccgg taggccggag ctaatccagg tcaggcaact
      601 taagaaccgc gacattcttg cgcgctgatt ccttttcaaa cactgcgtgt atcggatgcg
      661 ggcagtcttg ccgctattcg accccgcaac acattcattt acagccggtg acaaatttgg
      721 catttttttg gccaagcaat tgtccgacgc tctctcaaat cgccaacatg ataaaaaatt
      781 acttcctggt tgccgtccgt catttcctgc ggcatcgatt gtttgcgcta atcaatgttt
      841 tctgcctggc aattgggctc acattttcga tgctgatcgg cgtttacatc ttgcagcaaa
      901 ggagcgtgaa taccagtttg agaaacatag accggcagta tgttatcaag agtatttgga
      961 aagaaaagac catgggtatg gagcaaacca gtatcggccc tttggccgaa acgctgcata
     1021 aatcctatcc tggccttgtc gccaattatt tccggtacaa tcctgtcacc aacgtcgtgt
     1081 cggccgggga cagatatttc aaagagaaca ttgccatttg cgatacaacg ctgatcagca
     1141 tgtatggatt cccgctcctg tatggcgatg cgacaaaccc ctttcccacc gacaactcag
     1201 cggtcatcac ggaggctatg gccaaaaagc ttttcggaaa tgacaatccg atcggaaaag
     1261 cgattaacat actaacgatt gggcagggca atcagggcta caccgtttcc gcagtgctga
     1321 aatccatgcc gtataactcg gtgaacaacc tgattgataa gtatgggtat agtgtcttcg
     1381 tgccgtttgc ggggagccac tactatagta tggcaggcgg cgcaggccag gacgactgga
     1441 cacaaatttt catggtgagc atggtggagt tgaaacccgg tgttgctgcc ggcgatctcg
     1501 aggcgccggc gaaaaaaatc ctggcgctgc acttagccaa aaacatcaaa ggcttcctgc
     1561 agccggcttt cgcacctttg aaggactact acctgaatga caacaacggg gcggtgcggc
     1621 aaatgatcac cacgctttcg ataatcgccg tgtcattctg gctatggcga tcatcaattt
     1681 tgtcaacatc aatatcggca cttcctccta tcgcgtaaag gagatcggct tgcgaaaggt
     1741 atttggcggc gcccggaaac agctcatcgg gcaacacctt accgagtcga ttacgctgac
     1801 ggtcgttgcc gggctgatct cgcttggtct ttacgaaatc acccgtccgt attttagcca
     1861 agtcttgcat gtgccgatgg actcgttgtt cgcctttggc ggtttccagt atttattgct
     1921 ggcgacactc atcatcgtcg tcggctgcct ttcgggaatc tatccggcct tcgtcctaag
     1981 ttccactgac atcattcctt ccatcaaggg aaagatcgac acggggaggg gaagtttggc
     2041 cttgcggaaa gcattgttga tcacccaatt cacgctggcc gttacggttt ttatcagcgc
     2101 cttgaatgtc tccaaacaag tgtcttatat cttcaatcgg gacctgggat acaacaggga
     2161 acagttagtg gttttgactg tgttccccaa gcaatgggac tccgccggca ttcagcacat
     2221 ggtgacggtg agaaacgaac tggcaaacac accccatgtt catgatgctt ccgtatcgtt
     2281 cgaagtaccg gagcgcactc ctcccaatag cctcgcaatt gtgcgaacgg ggtcggacca
     2341 gactgtccag gtccctgcca tggtcgcgga tgaaaatttc ggcggcactt tcggaatgca
     2401 tcttgtcaaa gggaattttt tcgacgatta cagaagaggc gcaggcaagg atgagatcgt
     2461 tttgaatgaa acggccgcga aagcggcggg catcacggaa ccaaaccagg ccgttcgaac
     2521 aagccagggc ttcacgtata aggtctcggg aattgtgaaa gattacaatt attcatcatt
     2581 tgaacagcgg atcggcccgc ttgcgatatt gcacatgaaa gacaatacct cctaccggta
     2641 cctgacgtta aagatcgacg gccgcgacgc gcccgcgacc ttagccgccg tcgggaaaag
     2701 tggcgaaagc tggcgcctgg ctcacccttc gattacgatt tcatggatac acgattcagg
     2761 tccctgtatc agtcggaagt gcaactaaag caagcgacga atatggccac catcctcaat
     2821 ttggtcatcg tattcctagg cattttcggg atggttgcct tttcgctggc ccgccgcatc
     2881 aaggaaatct cgatccgcaa gattttaggg gccgaggcaa aaaacatcat tgccctgttc
     2941 ctaaaagaat atgcaatact gatcctgatt gcaaatatga tcggatggcc gctggcttat
     3001 ctggccaccg accactggct gagtaattat tcctatcgga tgcagcagga tatcgtgccc
     3061 tacgcttccg tgctggtgat cgtatttatg tctgcatttg tttttatttc ggctctgtgt
     3121 ttcagagtgg cgatcgccaa tcccgttcgg aatttaaaaa tggagtaaac ctacgtctcc
     3181 aatcgggcgc caggaaaggc cacagccggt agttttgaaa ggctatcata tccgcagatt
     3241 aattctattt caggttcttc tgtaaggcga attcacaaac aattatttcg ctcttgttgt
     3301 ccagatagga gatgacatac tttttgcgaa aattaatgca cc
//
LOCUS       DATGKE010000584         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_655837, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000584 DATGKE010000000
VERSION     DATGKE010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655837"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VKU83_09970"
     CDS             complement(<1..585)
                     /locus_tag="VKU83_09970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93927.1"
                     /translation="MNKRIIIAGGGTGGHIFPAIAIAGALQKQQPGIELLFVGAKGKM
                     EMEKVPQAGYRIEGLDITGFNRSSWLRNIGLPFQLIRSFFQVNRIFRRFRPDAVIGVG
                     GYSSFPVLRSAQAKRIPTFIHESNSFAGKSNILLGRKAARIFVASDGMEKFFPLDRIL
                     LTGNPVRPAIADSRITRDDAIRSFGLDPAKPTVLS"
     gene            complement(578..1813)
                     /locus_tag="VKU83_09975"
     CDS             complement(578..1813)
                     /locus_tag="VKU83_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLI93928.1"
                     /translation="MSNSPTIERIPFQSLRSRTRGDKVIWTAVFLLAIVSLLVVYSST
                     GLLAYKMYKGNTEIYLFKQFAFIVAGVAIIYFAHQVNYTIYSRVATILFLVSIPLLLY
                     TLFFGVKLNEGSRWIRLPIINMTFQTSDLAKLALFMYLSRLLSKKQDVIKDFREGFLP
                     VITPVAIICLLVAPANLSTALLIGATSLILLFIGRVSARHLLLSVAVAAVPVLILIGA
                     AMLRHHSNNDATEKPRSEGHSGLFVRVDTWISRVEAFIYGGGDSEDNNYQTNQAKIAI
                     AKGGFLGLGPGNSQQRNFLPHPYSDFIYAIIIEEYGMVGAVLIALIYLIFLLRSIRIF
                     RRCPFAFGAFLALGLSFTLVIQALANMAVNVNLFPVTGVTLPLVSMGGSSFLFTCLAI
                     GIILSVARNVEQLEGSASE"
     gene            complement(1810..>2777)
                     /gene="murD"
                     /locus_tag="VKU83_09980"
     CDS             complement(1810..>2777)
                     /gene="murD"
                     /locus_tag="VKU83_09980"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HLI93929.1"
                     /translation="CRHGGLDCALVGNIGRSFARQVAEDPRPWYVAEISSFQLDDIVS
                     FRPDIAVLTNITEDHLDRYEYKFENYIRSKFRIVTNQTAGDYFIYNADDPVISSYLSQ
                     SNLHSNPLPFTMQQEPNKGGYIRNGQMHINAGSERLQMSIYDFALRGIHNQYNTMAAG
                     IAAATVGIRKEKIREAIRSFEALEHRMEYVSTVRGVEFINDSKATNVNSTWYALESMD
                     RPTILILGGVDKGNDYSLLRDLVKEKVKAIVCLGADNRKIHEAFQNDVPLMVNTGSAD
                     EAVKAAFHFAVKGDVVLLSPACASFDLFKNYEDRGRQFKEAVRDL"
BASE COUNT          601 a          768 c          808 g          600 t
ORIGIN      
        1 ggacagcacg gtcggcttcg ccgggtcaag tccgaacgac cggatagcgt cgtcccgggt
       61 tatccggctg tcggcgatgg caggccggac cggattgccg gtgaggagga tgcggtccag
      121 gggaaagaac ttctccatcc cgtcgctggc gacaaaaatc cgcgctgcct tcctgcccag
      181 taggatattg ctcttgccgg cgaaagaatt gctttcgtgg atgaaggtgg ggatgcgttt
      241 tgcctgggcg ctccgcagga cgggaaagct ggaatagcct cccacgccaa tgacagcatc
      301 cggccggaaa cggcgaaaaa tgcggttcac ctggaagaag ctccggatca actggaaggg
      361 cagaccgatg ttccttagcc atgagctccg gttaaagccc gtaatatcca ggccttcgat
      421 ccggtagccg gcctggggca ccttctccat ctccattttg ccttttgcgc cgacaaaaag
      481 cagttcgatg cccggctgtt gcttctgcaa agcccccgca atggcgatgg ccgggaagat
      541 atgtcccccg gtcccgcccc ccgctatgat gatccgttta ttcactggcc gagccctcca
      601 actgctctac gttgcgcgcc acgcttagaa tgatcccgat cgcgaggcag gtgaacagaa
      661 aggaactgcc gcccatgctg accagcggca gcgtcacacc ggttaccggg aacaggttga
      721 cgttgacggc catattggct agcgcctgga tcacaagcgt aaaactcagg cccaatgcca
      781 ggaacgcccc gaatgcgaac gggcagcgcc ggaaaatgcg aatgcttcgc agcagaaaaa
      841 tcagataaat gagggcgatg agcacggccc cgaccatccc atattcctca atgatgatcg
      901 cgtagataaa gtccgaataa ggatgcggta aaaaattccg ctgctggctg ttcccgggac
      961 cgagtcccag aaagccgccc tttgcaatgg cgatctttgc ctggttggtc tggtagttat
     1021 tatcctcgct gtccccgccg ccatagatga acgcctcaac ccggctgatc caggtgtcca
     1081 cgcggacgaa gagccccgaa tgaccttcgc tgcggggctt ttcggtggca tcgttattcg
     1141 aatgatgccg gagcatcgct gcaccgataa ggatcagcac cgggacggcg gcaaccgcaa
     1201 cgctcagcag caggtgccgg gcactgacgc gcccgatgaa gaggaggatc agactggtag
     1261 ccccgatgag cagggcggtg gaaaggttgg cgggggcgac cagcaggcaa atgatcgcca
     1321 ccggcgttat caccggaaga aatccttcgc ggaaatcctt gatgacgtcc tgctttttgc
     1381 tgagcagccg gctcagatac atgaaaagcg ccagcttggc gaggtcggag gtctggaagg
     1441 tcatgttgat gatgggcagg cggatccacc ggctgccttc attcagtttt acgccgaaga
     1501 acaaggtata cagcagcagc gggatcgaca ccaggaagag gatggttgcg acgcgggaat
     1561 agatcgtata gttgacctgg tgggcgaaat agatgattgc cacgccggcc acgataaagg
     1621 caaattgttt gaacaggtag atctccgtat ttcccttgta cattttataa gcaagcagtc
     1681 cggtcgaact gtatacgacc agcaaagaga cgatggccag caggaaaacc gctgtccaga
     1741 tgaccttgtc gccacgggtg cgggaccgca ggctttggaa aggtatccgt tctatcgtcg
     1801 gactgttgct catagatccc tgacagcctc cttgaactgt cgtccccggt cttcataatt
     1861 cttaaagagg tcgaagctcg cgcaggccgg gctcagcagc accacatctc ccttcacggc
     1921 aaaatggaac gccgccttca cggcctcgtc ggcgctgccc gtattgacca tcagcgggac
     1981 atcattttgg aaggcctcgt ggatcttccg gttgtcggcg ccgaggcaga cgatcgcctt
     2041 tactttttct ttcacgaggt cgcggagcag ggaatagtca ttgcccttat ccacgccgcc
     2101 caggatcagg atggtgggcc tgtccatgct ctccagggcg taccacgtgc tgttgacgtt
     2161 ggtggccttt gaatcgttga tgaactccac gcctctgacc gtgctgacat actccatgcg
     2221 atgttccagc gcttcgaaac tccggatcgc ttcccggatc ttctctttgc ggatgccgac
     2281 agtcgcggct gcaatacctg ctgccatagt attgtattga ttgtgaatgc cgcggagggc
     2341 aaaatcgtaa atgctcattt gcagtcgttc cgatccggcg ttgatatgca tttgtccgtt
     2401 cctgatgtaa cctcctttat tgggttcctg ttgcatagta aatgggagtg ggttagaatg
     2461 taagttagat tggcttaaat agctgctgat gacggggtcg tccgcgttat aaatgaaata
     2521 atctccggct gtctgattcg tgacgatccg gaatttgctc cggatataat tttcgaactt
     2581 atactcgtac cggtcaaggt ggtcctcggt gatgttcgtc aggacggcga tgtccggccg
     2641 gaagctgacg atgtcgtcca gctggaagct gctgatctcg gcgacatacc atggccgcgg
     2701 gtcctccgct acctggcggg cgaaacttcg acctatattc ccgaccagcg cgcagtccag
     2761 gccgccgtgg cggcaga
//
LOCUS       DATGKE010000585         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1278635, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000585 DATGKE010000000
VERSION     DATGKE010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278635"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..572)
                     /locus_tag="VKU83_09985"
     CDS             complement(48..572)
                     /locus_tag="VKU83_09985"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLI93930.1"
                     /translation="MIALYIILGIIALILIIAAIAGTAINYEQSILINAPLSRVWDNI
                     KTLHAINQWNPWMDLDPNMKIQYGGTDGTPGAGFSWDSTVKNAGAGSQTILKVSDQSQ
                     LHTRIDFLRPFKNTAYADFNVAPDTGNTRVSWSLKGKMPYPMNIMKLTGAMQKNIGKD
                     FTKGLNKLKTICES"
     gene            613..2517
                     /locus_tag="VKU83_09990"
     CDS             613..2517
                     /locus_tag="VKU83_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HLI93931.1"
                     /translation="MRYFGFVFWCMWFTAAPAQVTPRNLLAPYVNKLSTLLNDKWYPF
                     PRTPEEWSRVVSDADRGHLVEAGEKALGFEFRPLPATLILEYKRTGNRVHFEKASFEK
                     RNALWALVLAESAEGKGRFTDKILDGVWSICEESYWGLPAHLGLQKAGTGLPDVNDPT
                     VDLFTAETAAVLAWTDYFAGAALDKLSPLVLPRIYSEVNRRVFVPLQTAKYGYLGGGR
                     TDVRLNNWDPWVMSNYCTAALLLEKDPARLVDAVGRAMHYTDLYINGLGEDGGCEEGP
                     SYWTAAGACVFDVLNLLSDATQGRIDIYHEPIVRRMAAYIYETHIAGKYYLDIADAPP
                     QFVPDGPMIFRFGRAIGDTVMEGFGSWITHMYPTGPGSYEQFHRTRALYDLLAAGDCE
                     RYPPAEPVMPRVWLSDVQLLIARDASGLFLATHAGTNGESHNHNDVGDFILYAGGRPV
                     IIDVGSGTYTARTFSSHRYDLWFNTSAYHNLPTINGYQQHDGIAYMATEVKCQGNALT
                     MDMAKAYPAEAGISHWVRSVRMDKGVSIVDRYQAAAPLRELTQSFLTVCAVDTSRAGR
                     IVFDAGGGIQVTLDYAAGNWTPAVEKVDFGIPEEAGVHEHWEGKPVYRVLLTARGMAA
                     AGVIRWSFHY"
BASE COUNT          518 a          621 c          888 g          642 t
ORIGIN      
        1 aggtggtaga gcggcggatg ctaaagcgat gaaggggacg gccggtgcta actttcgcag
       61 atcgttttga gcttattgag ccctttggtg aaatcctttc cgatattttt ttgcatggcc
      121 ccggtcagtt tcatgatgtt catggggtag ggcattttgc ctttcaggct ccagctgacg
      181 cgggtattgc cggtatccgg ggctacattg aaatctgcat aggcggtgtt cttgaagggc
      241 cgcaggaagt cgatccgggt atggagctgg ctttggtcac ttaccttgag aatggtctgg
      301 cttccggcgc cggcattctt taccgtgctg tcccagctaa agccggcacc cggggtacca
      361 tcggtcccgc cgtattggat cttcatattg gggtccaggt ccatccaggg gttccattgg
      421 ttgatggcgt ggagggtctt gatgttgtcc catacccggc tcagcggggc gttgatgagg
      481 atggactgtt cgtagttgat ggctgtgccg gcgatagcgg cgatgatgag gatcagggcg
      541 atgatgccga gaatgatgta taatgcgatc atattgcaag ggttttggtc cttaaaatta
      601 gaaaatcttt ttatgagata ttttggattt gttttttggt gtatgtggtt taccgcggcg
      661 ccggcgcagg tcactccgag gaatttgctt gcgccttatg tcaacaagct atctaccttg
      721 ttgaatgaca agtggtatcc gtttccccgt accccggagg aatggagccg ggtggtgagc
      781 gatgccgacc ggggacattt ggtagaggcg ggggagaagg cattgggttt cgaattccgc
      841 ccgcttccgg cgacgctgat cctggaatac aagcgcacgg gtaaccgggt acattttgaa
      901 aaagcctctt ttgaaaaacg caatgcttta tgggcgttgg tccttgcgga atctgcagaa
      961 ggcaaggggc ggtttacgga taagatcctg gacggggtgt ggtcgatctg cgaagaaagt
     1021 tattggggac tgcctgcgca tctcggctta cagaaggccg gtacggggtt acccgatgta
     1081 aacgatccca cggtagacct ctttacggcg gagacggccg cagtgctcgc ctggacggat
     1141 tattttgccg gggcggcgtt ggacaagctt tcgccgctgg tgctgccgcg gatctactcg
     1201 gaggtgaacc ggcgggtatt cgtgccgctg cagacggcga agtatggcta tttgggtggc
     1261 ggccggacgg atgtgcggct caacaactgg gatccctggg tcatgtcgaa ctattgcact
     1321 gcggcgctgc tgcttgagaa agaccctgcc cggcttgtgg atgccgtagg gcgggcgatg
     1381 cattacaccg atctttatat aaacgggctg ggggaggatg gaggatgtga agaggggccg
     1441 agttactgga cggcggcggg cgcctgtgtc ttcgatgtcc tgaatctgtt aagcgatgcc
     1501 acgcagggcc ggatcgacat ttatcatgaa ccgatcgttc gccggatggc ggcttatatc
     1561 tacgagacgc atatagcagg caaatattac ctggacatag ccgatgcgcc gccgcaattc
     1621 gtaccggacg ggccgatgat cttccggttc ggccgggcga taggcgatac cgtaatggaa
     1681 ggtttcggga gctggataac gcatatgtat cctacaggtc cgggctcgta tgagcagttc
     1741 catcgtaccc gggcactcta tgatctgctg gctgccggcg attgcgagcg ctatccgccg
     1801 gcggagccgg tgatgccccg ggtctggcta tcggatgtac agctgctgat agcgcgggat
     1861 gcctcggggc tattccttgc cacgcatgcg gggacgaatg gggaaagtca taaccacaat
     1921 gatgtggggg actttattct ttatgccggc ggccggccgg tcatcatcga tgtgggttcg
     1981 gggacgtata cggcacggac attcagctcg caccggtatg acttgtggtt caatacgagc
     2041 gcgtatcata atttgccgac tataaatggg tatcagcagc acgatgggat tgcctatatg
     2101 gcgacggagg tgaaatgtca ggggaatgcg ctaacgatgg atatggcgaa ggcgtatccg
     2161 gctgaggcgg ggatcagcca ttgggtgcgg agtgtgcgaa tggacaaggg cgtgagcatt
     2221 gtcgaccggt atcaggctgc ggcgccgtta cgggagctga cgcaatcgtt cctgacggtg
     2281 tgtgcggtcg atacgagccg ggccgggcgg atcgtattcg atgcgggagg gggtatacag
     2341 gtaacgttgg actatgctgc cggtaattgg acccctgccg tggaaaaggt cgatttcggt
     2401 attccggaag aagcgggcgt gcatgagcat tgggagggca agccggtcta ccgggtgctt
     2461 ttgacggctc gcgggatggc tgccgcgggt gtcattcgct ggagctttca ctattgagcg
     2521 accctatccc agatatctct ttcgaagatc aaaactgatt ccacgctgcc atcggggcct
     2581 ttccggacct ttaacgtcat gcccatcggt tcaaagaagc tggttgccga ttctgggagg
     2641 aggtctacgt ggcggccgtc ccagccttg
//
LOCUS       DATGKE010000586         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1125246, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000586 DATGKE010000000
VERSION     DATGKE010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125246"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2493
                     /gene="nirB"
                     /locus_tag="VKU83_09995"
     CDS             <1..2493
                     /gene="nirB"
                     /locus_tag="VKU83_09995"
                     /inference="COORDINATES: protein motif:HMM:NF011565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HLI93932.1"
                     /translation="DGIKPIVVVIGNGMVGHRFCEKLRSKGLYRIVVFGEESRFAYDR
                     VHLSEYFSGKSAAELSLSMAGWFEEEEILLHLGDPVREIDRQHKLVHSVKGVVQPYDL
                     LVLATGSSPFVPDIPGIEKEGVFVYRTIDDLDKMKAYASGAKCGAVMGGGLLGLEAAK
                     ALLDLGVPETHVVEFAPRLMPRQIDSAGSAALEASLRGLGLQLHLNKTTQSVEGHGRV
                     EALQFADESRLPIDMLVISAGIRPRDELARLAGLTVGTRGGIVVNEKLQTSDKYIYAI
                     GECALSQGMIYGLVAPGYEMADVVAANLSGGNKTFAGFDMSTKLKLIGVDVASFGDPF
                     TNEPEGRSIVFDNRQKGVYKRINISKDGKYLLGGILIGDAAAYNILLQTVNNRLVLPP
                     DPEDLILGSRDGGGEGAGVMRLPDEALICSCEAVTKATICSFVTEKGVETVNGIKKCT
                     KAGTGCGGCVPLIKDLITGTMKAAGIYVRNIVCEHFDRSRQELFDLIKLHEMHTYEEA
                     LTQWGKGDGCEVCKPVVASILASLWNETIAKQATIQDSNDRYLANIQKGGSYSVVPRI
                     PGGEITPEKLIVLGKVAKKYNLYTKITGGQRIDLFGAHVSDLPSIWEELIDAGFESGH
                     AYGKALRTVKSCVGSTWCRFGLHDSVSFAIEIEERYRGIRAPHKIKGGVSGCIRECAE
                     AQGKDFGIIATEKGWNLYVCGNGGSRPQHAQLLAADIDKESCVRYLDRFLMFYIRTAD
                     PLTRTATWLNKMEGGMSYLRNVVMNDSLGMGAEWEAEMQKLVDSYKCEWKEVVNDPEL
                     RKRFTHFVNAPRKKDPELSFDTMRGQIKAKEW"
     gene            2504..2848
                     /gene="nirD"
                     /locus_tag="VKU83_10000"
     CDS             2504..2848
                     /gene="nirD"
                     /locus_tag="VKU83_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HLI93933.1"
                     /translation="MDSTMIKWQAACRIEDVPAEGGACVLLEGEQIAIFHFARRGQWY
                     ATENRCPHKLQMALSRGMTGSAGENCEAKVACPFHKKTFSLETGECLSGDDLRIRTYP
                     VKIKNGKVYIGL"
     gene            2905..>3726
                     /locus_tag="VKU83_10005"
     CDS             2905..>3726
                     /locus_tag="VKU83_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HLI93934.1"
                     /translation="MQKIITGMAIYLLSSFSFKARAQLSLTGELRVRTEFRNGYGTLE
                     TVGATPAFVTSQRARLIFHYASPRVIFHASVQDVRVWGADASTINNADGARLGVHEAW
                     GELVLSNSADTSFGPSPVQWFSVRIGRQELVYDDERLLGNLNWLQQARRHDAVVFKLR
                     HNGWQVDAGAGFNQNTDAFNYNGTYYMPDNIPAYVKDSKGNLAPVPAGYVPLVNAAGL
                     SARTGSPSLVIAPSTNGANQDYKAMQFLYIAKQWHATRLAGLVFGDEFGRYKLDST"
BASE COUNT          826 a          890 c         1270 g          740 t
ORIGIN      
        1 gacggtatta aacccattgt agtagtcatc ggcaacggta tggttgggca tcgtttctgt
       61 gagaagctca ggtccaaagg tttataccgg atagtggtat tcggggagga atctcgtttt
      121 gcctatgacc gggtgcattt aagcgaatat ttcagcggta agtcggcggc ggagctgagt
      181 ttatcgatgg ccggttggtt tgaagaggag gagatcctgt tacacctggg cgatccggtc
      241 cgggagatcg accggcagca caagctggtc cattccgtca agggggtcgt acagccgtac
      301 gatctccttg tgctggcgac gggctcttcg ccttttgtgc cggacatacc ggggatagag
      361 aaagaggggg tattcgtata ccggacgatc gacgatctgg acaagatgaa ggcctatgcc
      421 tccggagcca agtgtggagc ggtgatgggc ggagggttgc tggggctgga agcggccaag
      481 gcgttgctgg atctgggtgt ccccgagacg catgtggtgg agttcgctcc ccggctgatg
      541 ccccggcaga tcgacagtgc gggcagtgcc gcattggaag ccagtctccg ggggctggga
      601 ttgcagctcc atttaaataa gacgacacaa tcggtagaag gccatgggcg ggtcgaggcg
      661 ttgcagtttg ccgacgaaag ccggctgccg atcgatatgc tggtgatctc ggcgggtatc
      721 cggccccggg atgagctagc ccggctggcg gggctcacgg tgggcacgcg cggcggcatt
      781 gtcgtcaacg agaagctaca gacctccgat aaatatatct atgccattgg ggaatgcgcg
      841 ctttcgcaag ggatgatcta tggactggtt gcgccgggct acgaaatggc ggatgtcgta
      901 gcagccaatc tgagcggcgg gaacaagacc ttcgcaggtt tcgacatgag cacgaagctg
      961 aagctgatcg gcgtggacgt agccagtttc ggggacccgt tcaccaatga gccggagggc
     1021 cggagcatcg tcttcgacaa ccggcagaag ggggtgtaca agcgcatcaa tatcagtaaa
     1081 gacggcaagt atctgctggg cggcatcctg atcggcgatg cagccgccta taatatcctg
     1141 ttacaaacgg tgaacaaccg gttggtgctt ccgcccgatc cggaggacct cattttgggg
     1201 agcagggatg gcggcggcga aggggccggc gtgatgcgtc tgccggacga ggcgctgatc
     1261 tgcagctgcg aggcggtgac gaaggccacc atctgttctt ttgtaacgga aaaaggcgta
     1321 gagacggtaa acggcatcaa gaaatgcacg aaggcaggaa cgggttgcgg aggctgtgtg
     1381 ccgctgatca aggacctgat cacggggacg atgaaggcgg ccggcatcta tgtgcggaat
     1441 atcgtttgcg aacatttcga ccggagccgg caggagctat tcgatctgat caagctgcac
     1501 gagatgcata cctatgagga agcgctcacg cagtggggga aaggcgacgg ctgtgaagta
     1561 tgcaagccgg tagtcgccag catcctggcc agtctatgga acgagacgat cgccaagcag
     1621 gcgaccatcc aggatagcaa tgaccgttat ctggccaata tccagaaagg cggcagctat
     1681 tcggtagtcc cgcgcatccc cgggggtgag atcacgccgg agaagctgat cgtgctgggg
     1741 aaggtagcca agaaatacaa cctgtatacg aagatcacgg gcggacagcg gatcgatctg
     1801 ttcggggcgc atgtgagcga tctgccatcg atctgggaag agctgatcga tgcgggtttc
     1861 gaaagcgggc atgcgtatgg gaaggcgctg cggacggtga agagctgtgt gggcagcacg
     1921 tggtgccggt tcggtctgca tgacagtgtc tcgttcgcca tcgagatcga ggaacgttac
     1981 cggggcatcc gggcaccgca taagataaag ggcggcgtaa gcggctgtat ccgcgaatgt
     2041 gcggaagcgc agggcaagga tttcgggatc attgccacgg agaagggctg gaacctgtat
     2101 gtatgcggca acggcggatc gcggccgcag catgcgcagc tgttggcagc ggatatcgac
     2161 aaggaaagct gtgtacggta tctggaccgg ttcctgatgt tctacatccg cacggcagat
     2221 ccgctcacgc ggacggccac gtggttgaac aaaatggaag gggggatgag ctatctgcgg
     2281 aatgtagtga tgaacgacag tctggggatg ggtgcggagt gggaagcgga gatgcagaag
     2341 ctggtcgaca gctataaatg tgaatggaag gaggtggtga acgatcccga gctgcggaag
     2401 cggttcacgc atttcgtgaa tgcgccgcgg aagaaggatc cggagctgtc tttcgacacg
     2461 atgcggggtc agatcaaggc gaaggagtgg taatattcga attatggatt caacaatgat
     2521 aaagtggcag gctgcctgcc ggatcgaaga tgtccccgcg gaggggggcg cgtgcgtatt
     2581 gctggagggc gaacagatcg ccatctttca ttttgcccgc cgggggcaat ggtatgcgac
     2641 ggagaaccgt tgtccgcaca agctgcagat ggccttgtcg cgggggatga cgggcagcgc
     2701 gggggaaaat tgcgaggcga aggtcgcatg tccttttcac aagaagacct tttcgctgga
     2761 gacgggggaa tgtctgagcg gggatgacct ccggatacgg acgtatccgg tgaaaataaa
     2821 aaatgggaaa gtatatatag gcctttagcg aggccaataa aattgccggt tgctgccata
     2881 acgatcttaa ccaaaacgac tgatatgcaa aagatcatca ccggcatggc catctattta
     2941 ctatcgagct tttcattcaa ggcgcgggcg cagctatcgc taacggggga gctgcgggta
     3001 cgtaccgaat tccgcaatgg ttacgggacg ctggaaacgg tgggcgctac gcctgctttc
     3061 gtgacctcgc agcgtgcgcg gctcattttc cattatgctt cgccgcgggt catcttccat
     3121 gcgtcggtac aggacgtgcg ggtgtggggc gcggatgcct ctaccataaa caatgcggat
     3181 ggagcgcggc tgggggtgca tgaagcctgg ggggagctag tcctttccaa ttccgcggac
     3241 accagcttcg gtccttcgcc ggtgcaatgg ttcagcgtac ggatcgggcg gcaggagctg
     3301 gtatacgacg atgaacggct cctgggaaat ctgaactggc tgcaacaggc ccgccggcac
     3361 gatgcggtag tcttcaagct ccggcacaat ggctggcagg tagatgcggg ggccgggttc
     3421 aaccagaata ccgatgcctt caactataac ggaacgtatt atatgccgga caatatccct
     3481 gcctatgtaa aggacagcaa gggtaatctg gcgccggtgc cggcgggtta tgtaccgctg
     3541 gtcaacgcgg cgggtctgag cgcccgtacc gggtcgccat cgctggtgat cgctccgagc
     3601 accaacggcg ccaatcagga ttataaggcc atgcaatttc tctatatcgc caagcagtgg
     3661 catgctacgc ggctggcggg tctggtcttc ggcgatgaat tcggccggta taagctggat
     3721 tcgacg
//
LOCUS       DATGKE010000587         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1502100, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000587 DATGKE010000000
VERSION     DATGKE010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502100"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VKU83_10010"
     CDS             complement(<1..817)
                     /locus_tag="VKU83_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLI93935.1"
                     /translation="MPYTELQVTSNFSFLRGASHPEELVKQAAAYGHHAIAITDRNTL
                     AGIVRAHDAARKIGIRLLPGCRLDLMDGPSLLALPTDKEAYARLSALLTSGNLRAEKG
                     ECRLFRADVYAHARGIQFILVPPGRLNAEFAYDEGFLSAAREYREAIGDLYLSACRYY
                     GGDDQKQLLRLNALATDLHIPLVATNDIHYHHPARRELQDVLTCIREKCTIYDAGFRL
                     HPNAERYMKPVEEMERLFRQYPRAIIRTLEIADKCHFSLDSLQYVYPEEITSDG"
     gene            complement(830..>2396)
                     /locus_tag="VKU83_10015"
     CDS             complement(830..>2396)
                     /locus_tag="VKU83_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLI93936.1"
                     /translation="TKDRLMGNRYCSLWFRHLLTDHLTIRRPALRERPFVLAAPDRGR
                     KRIIAVNSAAMREGIQHGMVVADARVLCPALEVIDAQAGYAEKLLRALAIWCIRYTPV
                     TAADGEDGLLLNISGCPHLWGSEEKYLDEICGRLKDKGYQVQGGIADTVGAAWAVARF
                     GSQEILRGSQGTLQSGHAIIPPGAQSATLLPLSLVALRLDAATFDRLQRLGLRRIGQI
                     AGMPRRALRRRFSSELLLRLDQAMGVAEENIEPVVPAEPFHERLPCLEPIVTATGIAI
                     ALQRLLDLMCKRLQDTGKGLRTAVFKGWRTDGKIIGVRIGTNRGSNNPGHLFTLFAEK
                     LGDLEPDLGIELFTLEAPKVEAVAAHQKTLWTGACGLEDRRLSELSDRISNKLGAGLI
                     HRYLPAEHYWPERSYKEAGTFDEAPASAWPKDRARPIHLLASPEPVVVAAPIPDYPPM
                     HFRYRNKIHTVVKADGPERIEREWWMDGGEGSKHRDYYVVEDEQGARYWLFRSGHYAG
                     GNGTQWWLHGFFP"
BASE COUNT          514 a          689 c          659 g          534 t
ORIGIN      
        1 ggccgtcgct cgtgatctct tcaggataaa catattgcag gctgtccagc gagaaatggc
       61 acttgtcagc gatctcaagg gtccttataa tggcacgcgg gtactgccgg aagagccttt
      121 ccatttcttc cacgggtttc atatacctct ccgcattcgg atgaagccgg aatccggcgt
      181 cgtaaatggt gcatttttcc cggatgcagg tcagtacgtc ctgcagctcg cgccgggcag
      241 gatgatggta atggatgtcg ttggtagcga ccaacggaat gtgaagatct gtagcgagcg
      301 cgttgagtcg tagaagttgt ttttgatcat ccccaccata atagcgacag gcggaaagat
      361 agagatcgcc gatcgcttcg cggtattctc ttgccgcgga aaggaagcct tcgtcatagg
      421 cgaactccgc gttcaaacga ccgggcggaa caaggataaa ctggatcccc ctggcgtgcg
      481 cataaacatc cgctctgaaa agccggcatt cacctttttc ggcacgcagg tttccggaag
      541 tcagcaatgc cgataggcgg gcataggcct ctttatccgt tggcaaggct aacagggaag
      601 gtccatccat gaggtccagc ctgcaacccg gaagcaggcg gatgccaatt tttcttgcgg
      661 cgtcgtgggc gcggacgatg cccgccagcg tattgcggtc tgttatggcg atagcatgat
      721 gaccataggc ggcagcctgc ttcaccagtt cctccggatg cgaagcgcct cgcaggaagc
      781 tgaaattgct ggtcacttga agttcagtgt acggcatatc gaaaggaatt taaggaaaga
      841 acccatgcag ccaccattga gtgccattgc ctcccgcata atggccggac cggaataacc
      901 aataccttgc cccttgttcg tcctcaacga cataataatc acggtgtttt gagccttcac
      961 cgccgtccat ccaccattcc cgttcaatcc tttccggacc gtcagctttg acgaccgtgt
     1021 ggatcttatt tcggtagcgg aaatgcatcg gtgggtaatc agggatgggc gcggcaacaa
     1081 ctaccggttc gggtgatgcc agaagatgga tcggccttgc ccggtccttt ggccaggcgc
     1141 tcgccggcgc ttcatcgaaa gtcccggctt ccttgtaaga ccgttccggc caataatgct
     1201 cggccggcaa ataccggtgg atcaatcccg cacctaattt gttgctgatg cggtctgata
     1261 gttccgacag ccttcggtct tccaggccgc aggcgcctgt ccacagcgtc ttttgatgcg
     1321 ccgcgacggc ttctaccttc ggcgcttcca gcgtgaataa ttcaataccg aggtccggtt
     1381 ccaggtctcc gagtttttcc gcaaagagtg tgaagagatg tccaggattg tttgacccgc
     1441 gattcgttcc tatccggacg ccgatgatct taccgtcggt ccgccatcct ttaaagaccg
     1501 cagttcgcaa tcccttgccc gtatcctgca agcgcttgca catgagatcc aataatcgct
     1561 gcagggcgat agcgattccg gttgctgtca cgataggttc caggcagggc aggcgttcgt
     1621 gaaagggttc ggcgggcacc acgggttcga tattttcttc cgctacgccc atcgcctggt
     1681 ccagccgaag caataattcg gaagaaaaac gccttctcaa tgcccgcctc ggcattccgg
     1741 cgatctggcc gattctcctg agcccgaggc gctgcaaccg gtcaaaagtc gcagcatcca
     1801 gcctgagtgc gacaagggaa agggggagga gcgtggcgct ttgcgctccc ggcggaataa
     1861 tggcatgacc gctttgtagc gtgccctgac tgccgcgcag aatttcctgg ctcccaaacc
     1921 gggctacagc ccacgctgcc cccaccgtgt cggcaatgcc accctgcact tgatatcctt
     1981 tgtccttaag ccggccgcag atctcatcga gatatttttc ttcgcttccc cagaggtgcg
     2041 ggcaaccgct gatattgagg aggaggccat cctcgccgtc agccgcagta accggggtat
     2101 aacggatgca ccaaatggca agcgctcgca gtagcttttc agcgtaaccg gcttgcgcat
     2161 cgataacttc cagggcagga caaaggaccc gcgcatcggc cacaaccatt ccatgttgaa
     2221 tcccttccct catggcggct gaattgacag cgataatccg tttccggcca cggtcagggg
     2281 cagccagaac aaatggcctc tcccgaagtg caggccggcg gatagtaaga tgatccgtca
     2341 gaagatgacg gaaccataag gaacaatacc ggttgcccat cagccggtct ttcgtt
//
LOCUS       DATGKE010000588         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_597549, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000588 DATGKE010000000
VERSION     DATGKE010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597549"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VKU83_10020"
     CDS             complement(<1..429)
                     /locus_tag="VKU83_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93937.1"
                     /translation="MRTFFKYLRPYRWVVMLALLLAAINQSFSMLDPYFFGKLGMDNL
                     AAHPYERGYYERQVTVKSDGSDGKPREQTRMVWKKTSPRSQAQFVWDVMRFLGIVIGV
                     AMVSRIAKAFQDYYGQIIVQKFGATIFTDGLRHSMGLPFTE"
     gene            complement(493..1776)
                     /locus_tag="VKU83_10025"
     CDS             complement(493..1776)
                     /locus_tag="VKU83_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLI93938.1"
                     /translation="MRSRQQRLFFYSVVAAIFTTLFSWFRINSLCIMLMVGTRYLYGP
                     LPLLRSVFTRRIFLAFLAYCLAGAAGFLYARNIPAQADLVAKEGTLIAFALVFCLPGS
                     FSRAGYRKLLDWYYFLLVAASVYCLAEAALNYRISGDSSVFFYHLLTRPIAQNAVFYS
                     VYIIFGLIFLLAPGGEPYAGRFTPGFRKLLRYALAGFYLGMLILLNSKLALVIGLIVL
                     LNAVFRRYSFRTHPRPLLATASVLVVLVGLLAMTNNPISRRYRELAAGDLAVVRQEKF
                     NPGMYINAMQLRLLEWRFGYEILRERKAWLFGVSPGDSQDLLDDKYVAANMYIGNPAD
                     GPHRKVRGFIGYNFHNQFLETLVKSGFVGLAALAAIFVVFFTDARRRGTREGWFVVLT
                     LALFFIPEAPLTMQHGIFLFCFFPLLSLYVPKNVE"
     gene            1874..>2221
                     /locus_tag="VKU83_10030"
     CDS             1874..>2221
                     /locus_tag="VKU83_10030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93939.1"
                     /translation="MNTRECPMVTIITIVYNGEKHIAETMESVGGQTYPNIEYIIVDG
                     GSTDNTLSIVDRFKDKVTHVISERDRGISDAFNKGIRMAKGGIIGIINADDWYEKDAV
                     EKIVGRIEGYDVAY"
BASE COUNT          594 a          589 c          592 g          446 t
ORIGIN      
        1 ttccgtgaac ggcaggccca tggaatgacg gagcccgtcg gtgaaaatgg tcgcgccaaa
       61 cttctggaca atgatctgac cgtagtagtc ctggaacgcc ttggcgatcc gcgaaaccat
      121 cgcgacgccg atcacgattc ccaggaaccg catgacgtcc cagacgaact gggcctgtga
      181 ccgggggctg gtcttcttcc ataccatgcg cgtctgttcc ctgggcttgc cgtcgctgcc
      241 gtcgcttttt accgtcacct gacgctcgta atacccgcgc tcatacggat gcgctgccag
      301 gttgtccatc cccaatttgc cgaagaagta gggatcgagc atcgagaagg actggttgat
      361 cgccgccagc agcaatgcca gcattaccac ccatcggtaa ggtcgcaggt atttgaaaaa
      421 ggttctcatt tttttcacct ggcgaaagat cgcttcgggg ataccatttc gcaaaaataa
      481 ggaagtacag ggctattcga catttttggg aacataaagc gacaggaggg gaaagaagca
      541 aaataggaat atcccatgct gcatcgttag cggcgcctcc gggataaaaa acaatgcaag
      601 cgtcagcacg acaaaccagc cctctcttgt cccacgccgg cgtgcatccg tgaagaagac
      661 gacgaatatc gctgcgaggg cagcaagacc gacgaatcca ctcttcacca acgtctccag
      721 gaactggtta tggaaattat agccgatgaa accgcgcacc ttccggtgcg ggccatcggc
      781 aggattaccg atatacatgt tggccgccac atatttgtca tcaagcaagt cctggctatc
      841 ccccgggcta acaccaaaca accaggcctt ccgctccctg aggatctcat atccgaagcg
      901 ccattccagc aggcgaagtt gcatggcatt gatatacatt cctggattaa atttttcctg
      961 ccggaccacc gccaggtctc cagccgcgag ctccctgtac cgtctgctga tgggattgtt
     1021 ggtcattgcc agaaggccga ccaggacgac cagaaccgaa gcggtagcca gcaagggccg
     1081 ggggtgggtt cgaaaggagt atctccggaa tacggcattg agcaagacga tgagtccaat
     1141 caccagggcg agctttgaat tgagcaggat cagcattcca aggtaaaagc cggccagcgc
     1201 gtaacgcagc agctttctga agcctggcgt aaatctgccg gcatagggtt ctccgccggg
     1261 cgcaagaagg aatatcagcc cgaaaatgat gtacaccgaa tagaacactg cattttgagc
     1321 aatgggccgg gtcagcaaat gataaaagaa cacggagcta tcccccgaga tccggtagtt
     1381 aagcgccgcc tccgcgaggc agtataccga agccgctacc aacaggaaat aataccaatc
     1441 cagcaacttc cggtaacctg ccctggagaa acttcccggc agacaaaata ccagcgcaaa
     1501 ggcgataaga gtgccttctt tcgctaccag gtcggcctgg gccggaatat tccgggcata
     1561 cagaaaaccg gctgctccgg ccagacagta agccaggaag gccaggaata tcctcctggt
     1621 aaatacggat cgtaaaagcg gcaagggccc gtacagatac cgggtgccga ccatcagcat
     1681 gatgcagagg ctgtttatgc ggaaccagga gaatagcgtt gtgaagattg cggcaaccac
     1741 cgaataaaag aacagtcttt gctgccgtga acgcatgtgg attacgcctc agatttccgg
     1801 caaattaggg ctttttcgca tttttgggcg atctttgtcg gctcaatttg taataaacaa
     1861 cgggttttca tctatgaata cacgtgaatg tccgatggta acgatcatca ccatcgtgta
     1921 caacggcgaa aagcatatag cggagacgat ggagagcgtt ggcggccaaa cctatcccaa
     1981 cattgaatac attatcgtcg atggtggatc cacggataac accctttcca ttgtcgaccg
     2041 gttcaaagat aaggtcactc acgtcataag cgagagggat cgcgggataa gcgatgcgtt
     2101 caataaaggc attcgcatgg caaagggcgg aatcatcggg atcattaacg ccgacgactg
     2161 gtacgaaaaa gatgccgtgg aaaaaatcgt tggccggatc gaaggctacg atgtcgctta
     2221 c
//
LOCUS       DATGKE010000589         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_263748, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000589 DATGKE010000000
VERSION     DATGKE010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263748"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VKU83_10035"
     CDS             complement(<1..209)
                     /locus_tag="VKU83_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93940.1"
                     /translation="MLLFALAIAPGVAICLFIYSMKKIGRASMPYLVICFLLGMAATV
                     PALGLQSLAVDPRLRPWRHSILFFAR"
     gene            complement(213..1751)
                     /locus_tag="VKU83_10040"
     CDS             complement(213..1751)
                     /locus_tag="VKU83_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93941.1"
                     /translation="MMTLRNVGSKILSNNKESLLAAIAGFGMIQLLCRHSGIGLSPDS
                     VVYLSTAQNIHDHGLINDYTNQPMMDFPAGYPIFLSGLIFLTGHAVTWFGPVLNGILF
                     GILIWVAGWMMDRFSWPSRWYKWTILIFLILSPCLLEVYSMIWSETVFLLLTLFFMIG
                     CHRYFRTHSIPALIAVAVVAGLACVTRYAGISVIAMGGLLMLCDRALPWGLRKLAHIG
                     LYGIVAILLPALNLYRNYRVTETYTGYREKAIRSLGVNLHDAGSVLCDWMPFFNGRYA
                     LATAVALIFILGISGFFVYRVARNPRRFSYETIAMSYFVTYAVFILGIASVSRFQQLD
                     SRLLSPLFIPWLWGSTCWIPTATKGLHSRPRLGWAVALMAAAGCFLYGEYDTFEANWE
                     GIEYAGIPGYMEDSWQQSATMAYVKSHKAELEGKGPIYSSATDGLWFLAGVPGELMPH
                     AENQEDIGYMMKQDHFTVIWFDDSVNTDLIDVNFIKKRKQLVGELHFDDGAIYFFRTP
                     KSSS"
     gene            1817..>3511
                     /locus_tag="VKU83_10045"
     CDS             1817..>3511
                     /locus_tag="VKU83_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93942.1"
                     /translation="MNVARLAFGIICICGSLGLSAQQFGGNPPSLKWRQINTDTARII
                     FPRGLDSAAQQVAAIVHRLSAATLPTIGPVQHKINIVLENQTTIANGYVSLAPFRSEF
                     QVTPEQNSFDLGSLPWNKMLAIHEYRHVQQYNNFRVGLSAALYYLFGEGGQALANSLA
                     VPNWFWEGDAVYQETLVSHQGRGRLPYFFDGYRGLWAGNRHYSWMKLRNGSLRDYVPD
                     WYPLGYMLVAYGRQTYGDDFWRNVGRDAAAFRPLFYPLQGAIRRYSGIPFTEFRTRAL
                     DHFRATLPDEASAAADRFASAHRHFDADEEFPQFAGPDRLLYMRTSYKQIPEFRLRDL
                     RDGKEYKVRTRGVSLDNYFSFADGQVVYAAYETDPRWNWRDYSVIRSLSLRTGDDRRL
                     TSATRYFSPDISADGRRIVTVMEGADGDCELRLLDGTSGELLRRIPNPDSLFYTYPKF
                     AGPERIVSAVRNGAGQMSIALMNAESGAVQYLLPFSYQTVGYPSVRGDTIWFTASRQG
                     QDRLYMLAGKRVFRVVTVHGEPFTGQYGFIAGPDGHYAWNDFTAAGYHIDTAASVLL"
BASE COUNT          781 a          959 c         1042 g          729 t
ORIGIN      
        1 cgggcaaaaa agagaatgga atgacgccaa gggcgcaaac ggggatccac tgcaaggctc
       61 tgcaatccga gcgccgggac ggtggcggcc atgcccagca gaaagcagat gaccaggtaa
      121 ggcatcgaag cgcgcccgat ctttttcatc gaatagatga acaggcaaat cgccacaccc
      181 ggcgcgatgg ctagtgcgaa taggagcatg gcctaagagg aggatttggg ggtgcgaaaa
      241 aaataaatgg cgccgtcgtc aaaatgaagt tcgccgacga gttgcttccg ctttttaatg
      301 aaattcacgt cgatgagatc cgtgttgacg ctgtcgtcga accagatgac ggtaaaatgg
      361 tcctgcttca tcatgtagcc gatgtcttcc tggttctcgg cgtgcggcat cagctcgccc
      421 ggcacgccgg cgaggaacca gagaccgtcg gtggcgctcg aatagatcgg gcctttgccc
      481 tccagttccg ctttatggga cttgacatag gccatcgtag cggactgctg ccagctatcc
      541 tccatgtatc ctgggatccc cgcatattcg atgccttccc aattcgcttc aaatgtatcg
      601 tattctccat ataggaaaca tcccgccgct gccatcagcg cgaccgccca tccaagcctt
      661 ggtcgactgt gcaggccttt tgttgcagta ggtatccagc aagtgctgcc ccatagccat
      721 gggatgaaaa gaggggagag cagtcggcta tccaattgct ggaaccgcga tacggaggcg
      781 atgccgagga taaaaaccgc ataggtgaca aaataactca tggcaatcgt ttcgtaggag
      841 aagcgacgcg ggtttctggc gacccgataa acaaaaaaac cgctgatgcc caggatgaag
      901 attagcgcaa cggcggtcgc aagggcatac cggccattga aaaagggcat ccagtcgcaa
      961 agcacgctgc cggcatcgtg caggttgacg ccgagtgagc ggatggcttt ttcccggtac
     1021 ccggtatacg tttcggtaac gcggtagttc cggtacaggt tcagggccgg caacaggatg
     1081 gccacgatgc cgtacagtcc tatatgggcc agcttcctca gaccccaggg caaagcgcgg
     1141 tcgcaaagca tgagcaatcc gcccatggcg atgacgctga tcccggcata gcgcgtaacg
     1201 caagccaggc cggcgaccac tgcaacggca atgagggcgg gaatggagtg ggtccggaag
     1261 tagcggtggc aaccgatcat gaagaacagg gtaagcagga gaaagaccgt ttctgaccag
     1321 atcatcgaat atacttccag caggcagggg ctcaggatga gaaagatcag gatcgtccat
     1381 ttgtaccagc gggacggcca gctgaaccgg tccatcatcc agccggcaac ccagatgagg
     1441 atgccgaata aaatgccatt caggaccggg ccgaaccagg tgacggcatg tccggtgagg
     1501 aatatgagcc cgctgagaaa aatcggatag cctgccggaa agtccatcat cggctgattt
     1561 gtatagtcat tgatcaggcc atgatcgtgg atattctgcg ccgtgctgag ataaacgacc
     1621 gagtcaggtg aaaggccgat gccgctgtgg cgacaaagga gttggatcat accgaaacct
     1681 gctatcgccg ccagcaggct ctccttgtta ttcgataaaa tcttcgatcc gacattgcga
     1741 agcgtcatca tatgcgaaag taagagattc ccgtacttaa cacaggtaat cggttttttt
     1801 tcctaatttg acccgcatga atgttgcccg cctggctttc gggatcattt gtatctgcgg
     1861 ttcgctcggg ctttccgccc agcagttcgg cggcaatcct ccttccctca aatggcggca
     1921 gatcaatacg gacaccgcgc ggattatttt tcccagaggg ctggattcgg ctgcacaaca
     1981 ggttgccgcc atcgttcacc ggctgagcgc cgcgacactg ccgacgatcg gccccgtaca
     2041 gcacaagatc aacattgtgc tggaaaacca gaccacgatc gccaacggtt atgtctcgct
     2101 cgcgcctttc cggagcgaat tccaggtcac ccccgaacaa aacagtttcg atctcggcag
     2161 tttgccctgg aacaaaatgc tcgctatcca cgagtaccgc cacgtgcagc agtataataa
     2221 ctttcgcgtc ggcctctccg ctgcgctcta ctacctcttt ggtgaaggcg gccaggcgct
     2281 ggctaacagc ctggcggtgc ccaactggtt ctgggagggc gacgccgtct accaggagac
     2341 cctggtgagc catcagggtc gtggcaggct gccgtatttc ttcgacggct atcggggttt
     2401 atgggcagga aaccggcact actcgtggat gaagctgcgg aacggatcgc ttcgggacta
     2461 tgttccggac tggtatccgc tggggtatat gctggttgcc tacggccgcc agacctatgg
     2521 tgacgatttc tggcggaacg tggggcggga cgccgctgcc ttccggccgc tgttttatcc
     2581 cctgcaggga gctatccggc gatacagcgg aatccctttc accgagttcc gcaccagggc
     2641 gctggatcat ttccgggcca cgctaccgga cgaagcttca gctgccgccg accggttcgc
     2701 gagtgcccac cggcattttg acgccgatga agaattcccg caatttgccg gtccggatag
     2761 gttgctgtat atgcggacct cctacaagca aataccggaa ttccgcttgc gagaccttcg
     2821 cgatggcaag gaatacaaag ttcggaccag gggcgtctcg ctggataatt acttttcgtt
     2881 tgctgacggc caggttgtct atgcggccta cgagaccgat ccccggtgga attggcggga
     2941 ttacagcgtg atccggtccc tcagcctgcg gacgggtgac gatagaaggc tgacgagcgc
     3001 gacccgttat ttttctccgg atatttcggc cgatggccgg aggatcgtca ccgtaatgga
     3061 aggcgccgat ggggactgcg aactgcggtt gctggacgga acctcggggg agctgctgcg
     3121 gcggataccc aatccggata gtttgttcta cacctacccg aaatttgcgg ggccggagcg
     3181 aatcgtttcg gccgtccgca atggggcggg tcagatgtcg attgccctga tgaacgcgga
     3241 aagcggggcg gtgcagtatc tgttgccgtt cagttatcaa accgtcggct atccttctgt
     3301 tcgcggcgat acgatctggt tcactgcttc gcggcaaggg caggatcgcc tgtatatgct
     3361 cgccgggaaa cgggttttcc gggtggtcac ggtgcatgga gaaccattca cgggacaata
     3421 tgggttcatc gcgggacccg atgggcatta cgcctggaat gattttaccg cagcgggcta
     3481 ccatatcgat acggcggcga gcgtcttgct c
//
LOCUS       DATGKE010000590         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1278759, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000590 DATGKE010000000
VERSION     DATGKE010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278759"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VKU83_10050"
     CDS             <1..936
                     /locus_tag="VKU83_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HLI93943.1"
                     /translation="YHIVTPAMHKSKEDVAKLFYEKLNTRPGLTPEQLTLVAREKLRK
                     KYVQAEIGITGANFIISDIGGIAVTENEGNARLSASFPKTHIVIAGLEKVIPTLADLG
                     LFWPLLATYGTGQRVTVYNTVITGPRQPGEADGPDEMILILLDNGRTNILANAKARES
                     LYCIRCGACLNACPVYKNIGGHSYGTTYSGPIGSVITPHLKEMQEWKHLSYASSLCGN
                     CTEVCSVKINLHELLLDNRYEAVQDGYATWQEKLAWKVWRKASLSRSLMNMGSRKTKN
                     WVVKKAFGAWTTHRDMLDFAPKTFNERWRSERKGR"
     gene            936..2249
                     /locus_tag="VKU83_10055"
     CDS             936..2249
                     /locus_tag="VKU83_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLI93944.1"
                     /translation="MLLFSTHITPRLNYVLDFFGKELFDDASAIRVTDDKETFLATHG
                     PRINYSNEVVPGAFTVRPAGLLFETGIRPLEVPCARSAGQPVLFPTHGDLSFDVFAAA
                     FYLLSRYEEYLPHPKDEYGRFPHSHSLAFREGFLDQPVINNWLMDLRRALGARFPDLV
                     FRYAVFKFIPTYDIDSAYSYLYKGWLRNLGGLLKSLARGQWKKAFDRLETLRGHRKDP
                     FDVYEWLDSLHLYCRMRPYYFFLAAARIQGVDRNIPISGPGLQDLIRYHATGSRVGIH
                     PSWQSGDDENLLKAEIEWMSYVSGQKMVRSRQHYIRFLLPTTYRLLIRYGVEQDFSMG
                     YGSINGFRASVASSFYWYDLGKETTTPLRIFPYCFMDANSYYEQGQTPAATLAELMGY
                     YRRIRQVNGLMVTIWHNSILGRDPEFAGWRGVYETFLKEEVFWDM"
BASE COUNT          528 a          707 c          659 g          533 t
ORIGIN      
        1 tatcatattg taacacccgc catgcacaaa agcaaggagg atgtcgcgaa actattttac
       61 gaaaagctga acacccggcc aggcctcacc ccggagcagc tcaccctcgt ggcaagagaa
      121 aagctccgca aaaaatatgt ccaggcagag atcggcatca ccggcgccaa tttcatcatc
      181 tccgatatag gcggcatcgc cgttaccgaa aatgaaggca atgcgcggct gagcgcgtcc
      241 tttcctaaga cgcatatcgt catcgccggc ctggaaaagg tgatccctac gctcgccgac
      301 ctgggattgt tctggccgct gcttgctact tacggcaccg ggcagcgggt aaccgtttac
      361 aataccgtga tcacgggacc gaggcaaccc ggcgaagcgg acgggccgga tgagatgatc
      421 ctgatcctcc tcgataatgg ccgcaccaat attctcgcca acgccaaggc gcgcgaaagc
      481 ctatattgta tccgttgcgg cgcctgcctg aatgcctgcc cggtctataa aaacatcggc
      541 ggccacagct acgggaccac gtacagcggg cccatcggct cggttatcac gccgcatctg
      601 aaagagatgc aggagtggaa gcatctgagc tacgcctcat cgctttgcgg caattgcacc
      661 gaggtctgct ccgtgaagat caaccttcat gaattgttgc tggataatcg ctatgaagcc
      721 gtgcaggacg ggtatgccac ctggcaggaa aagcttgcct ggaaggtctg gcgaaaggcc
      781 agcctcagcc gctccctgat gaatatggga agccggaaga cgaagaactg ggtcgtgaaa
      841 aaggcgtttg gcgcgtggac cacgcaccgg gatatgcttg atttcgcgcc aaagaccttc
      901 aacgagcgct ggcgctccga acgcaaaggt cgttgatgtt gcttttcagc acccatatca
      961 ctccccggct gaattatgtc ctggattttt tcggaaagga gcttttcgac gacgcctccg
     1021 ccatccgggt cactgacgac aaagagacat ttctcgcaac ccacggcccg cggatcaatt
     1081 actcgaatga agtcgtcccc ggcgctttca cggtgcggcc tgcgggtctg cttttcgaga
     1141 caggcattcg cccattggaa gttccatgcg ccaggtcagc cggtcagccg gtcctgtttc
     1201 cgacccatgg cgatctctcc tttgacgtat tcgccgccgc cttctacctg ctgtcgcgat
     1261 atgaagagta cctcccccat ccaaaagacg aatatggacg cttcccacac agccattcgc
     1321 tggcatttcg cgaaggcttc ctcgaccagc cggtgatcaa taactggctg atggatcttc
     1381 gtcgcgctct tggcgcccgc tttcctgacc ttgtattccg ttacgccgtc ttcaagttca
     1441 ttcccaccta tgatattgat tcggcctatt cttacctgta taaaggatgg ctcaggaacc
     1501 tcgggggctt gctgaagtcg cttgccaggg ggcagtggaa aaaggctttc gaccggctgg
     1561 agaccttgcg cggacacagg aaagacccct tcgacgtcta tgaatggctg gactccctgc
     1621 acctctattg ccggatgcga ccctattatt tttttcttgc cgctgcccgc attcaaggcg
     1681 tcgataggaa cattcctatc tccgggccgg gcttacagga cctgatccga tatcatgcaa
     1741 ccggctcccg ggtaggcatc cacccgtcct ggcaaagcgg cgatgacgaa aacctgctga
     1801 aagccgaaat tgaatggatg agttatgtca gtggtcaaaa gatggtgcgg agccggcagc
     1861 attatattag gtttttgctt ccgacaacat accgtcttct gatccgctac ggggtcgaac
     1921 aggacttttc gatgggctac ggctcgatca atggatttcg cgcttccgtc gcctcttcgt
     1981 tttattggta tgacctcggg aaggagacaa cgaccccgct ccgcatcttt ccatattgtt
     2041 ttatggacgc caattcctat tatgagcaag gtcaaacccc cgcggcaact ttggccgagt
     2101 tgatgggcta ttaccggcgg atccgccagg tgaacggcct catggttacc atttggcaca
     2161 attccatcct tggccgcgac cccgagttcg ccgggtggcg cggcgtatac gagacctttc
     2221 tcaaagaaga ggtgttttgg gacatgtaga gattagcgca gggctaaacg ccctccggga
     2281 ggccagcctt acggctggcg atacgctaat gatggccctg ctggcgcagg gctaaacgcc
     2341 ctccgggagg ccagcctgac ggctggcgat acgctaatga tggccctgct ggcgcagggc
     2401 taaacgccct ccgggaggcc agcctta
//
LOCUS       DATGKE010000591         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_759414, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000591 DATGKE010000000
VERSION     DATGKE010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759414"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VKU83_10060"
     CDS             complement(<1..415)
                     /locus_tag="VKU83_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLI93945.1"
                     /translation="MSVIESLNWRYATKRMTGEKVPPEKVHAILEAARLAPTSSGLQP
                     FTIISVTDRQLLEQIKPIANNQPQITEASHVLVFAAWDDVSPAQIEKVFDQIIRERKL
                     PPDAMDRYKNWLSGYFARLTPEERFHAAARQAYLAF"
     gene            complement(441..1094)
                     /locus_tag="VKU83_10065"
     CDS             complement(441..1094)
                     /locus_tag="VKU83_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLI93946.1"
                     /translation="MGITDRKQRQKDEVRASILETAWEIILSEGWQSFSIRKVADAIE
                     YSVPVIYSHFENKDAILLEFSKKGFQLLADSLLLAKQSKGDPAEQIRAMGRAYWNFAF
                     ANKEYYQLMFGLGIPTCDTASRIPALADLHRVVTESLVAMAGPGRKPAFDPWLKYQSF
                     WSMLHGLVSINMVSPGRAEKPGGNRADADHVPLPQKVLEDVICSFIRGIEVAGTEKD"
     gene            complement(1236..1682)
                     /locus_tag="VKU83_10070"
     CDS             complement(1236..1682)
                     /locus_tag="VKU83_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93947.1"
                     /translation="MKKIIFGCCLVSASLLICTAAGAMDPVKVPMKVKAVFHHEYKDA
                     QDVRWTMRDRDYTVSFRKDGTREMAQYDWTGHRLDTRVAVPENALPDKVVSHLDKKYH
                     GNYSHSGTLIERPWKKDLYMVNVHQKGKSVPVYMDKKGHEHGYARR"
     gene            1837..>2120
                     /locus_tag="VKU83_10075"
     CDS             1837..>2120
                     /locus_tag="VKU83_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93948.1"
                     /translation="MNLTATSPTPAASPAARSTATSPLTKSPRLYSLDALRGFDMFWI
                     IGGEEIFHTMAKIYPNSPFWHALSDQFEHPLWNGFHFYDLIFPLFLFIAGV"
BASE COUNT          445 a          574 c          535 g          566 t
ORIGIN      
        1 cgaatgcgag ataggcctga cgtgcggcgg cgtggaaacg ctcttccggc gtcaggcggg
       61 cgaaatagcc cgagagccag ttcttgtacc ggtccatggc atccggcggc agtttccgtt
      121 cgcggattat ctgatcgaaa actttttcta tctgcgcggg cgatacatcg tcccaggcgg
      181 caaaaaccaa tacatgcgac gcttccgtaa tctgcggctg gttgttggcg atcggcttga
      241 tctgttcgag caattggcga tcagtgacgg aaatgatcgt gaagggctgc agaccgctcg
      301 aggtcggtgc aagccttgca gcttcgagga tcgcgtgaac cttttccggc ggtacttttt
      361 cgccggtcat tcgcttcgtg gcataacgcc agttcaatga ttcgataaca ctcatattcg
      421 ttttttaatg ttttttagtc ttaatctttt tcggttccgg caacttctat tccccggata
      481 aaactgcaga tgacgtcttc caaaaccttt tgcggcagcg gaacatggtc tgcatcggcg
      541 cggtttccgc ctggtttttc ggcgcggccg ggggacacca tgttaatgga gaccagccca
      601 tgtaacatgg accaaaagga ctggtatttg agccacggat caaaagcggg ctttctgccg
      661 gggcctgcca tcgccaccag gctttccgtg acgacacggt ggagatcagc cagggcgggt
      721 atccggcttg ccgtgtcgca agtggggatg ccgaggccaa acatcagctg gtaatattct
      781 ttgttggcaa aggcgaagtt ccaatacgcc cggcccatcg cccggatctg ctccgccggg
      841 tcgccttttg actgcttcgc caggaggagc gaatcggcta gcaattgaaa tcctttcttg
      901 ctgaattcca gcaaaatggc gtctttattt tcaaaatgac tgtagataac cggaacgctg
      961 tattcgatcg catcggccac cttacggatc gagaacgact gccagccctc gctcaaaatg
     1021 atctcccagg ccgtttccag gatgctggcg cggacctcgt ctttttggcg ttgttttcga
     1081 tcggtgatac ccaactttat atccagtagt ttttataaca ctgttagcaa atctacggcc
     1141 gttttaaaag ctgaccaaat atttttccgg cccaggtccg gacaaaaaaa agaggcgatc
     1201 catcggacca cctcctttcg cgcttgccgt cattcttacc ggcgagcata tccgtgctcg
     1261 tgccccttct tgtccatgta gacgggaacg gatttccctt tttgatgaac gtttaccata
     1321 tacaggtcct ttttccaggg ccgttcgatt agggtaccgc tatgggaata attgccatga
     1381 tactttttgt ccaggtgact aactaccttg tccggcaagg cgttttccgg aaccgcaacc
     1441 ctggtgtcca gccggtggcc ggtccagtcg tattgcgcca tctcccgggt cccgtctttc
     1501 cggaagctaa cggtataatc gcggtccctc atggtccagc ggacgtcctg cgcatcttta
     1561 tattcatgat ggaagaccgc tttaactttc atcggaactt ttaccgggtc catggctccg
     1621 gccgcggtgc agatcagcaa actcgccgaa acgagacaac atccgaaaat gatcttcttc
     1681 ataatccttg agtttaattt atcaaaatgg gtctcgggcc atgaaaatcg attcaagaat
     1741 cctgccaggg ggcggccccc gtagcggcag gtttagagcg gtattttttc tacaatttgt
     1801 tgcaggcttg ttaaatttcc tatattcatg gtccatatga atcttacagc aacctccccg
     1861 acgccggctg cgtcgccggc agcccgatcg accgcgactt ctcccctgac aaaatcaccg
     1921 cgactgtatt cgctggacgc cctccgcgga ttcgacatgt tctggatcat cggtggagaa
     1981 gagatctttc atacgatggc aaaaatttat ccgaacagtc ctttctggca tgcgctttcc
     2041 gaccagttcg agcatccgct ttggaacggc ttccattttt atgacctgat ctttccgtta
     2101 tttcttttta ttgccggcgt
//
LOCUS       DATGKE010000592         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_892702, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000592 DATGKE010000000
VERSION     DATGKE010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892702"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VKU83_10080"
     CDS             <1..749
                     /locus_tag="VKU83_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93949.1"
                     /translation="PKAYEAEAIRDRKVKVLKQVRMLTTQQVFRNVVRAQYTEGEING
                     EHRPAYRQEAQVAPDSKTETFVAARFFIDSQRWQGVPFFLRTGKSMPRQSSVIMVQFK
                     DSPHKIFRDDVVPNRLVISIQPELQIGLVFESKVPGLHMKLIPVEMDFTYRENYSQSL
                     PEAYETLLLDVLMGEATSFMRTDQIEAAWKLVMPILNAWKKYPGKQLQFYPAGTWGPA
                     AADKLLKPFAKDWFKLTAAPRVAQNMASIE"
     gene            822..2672
                     /locus_tag="VKU83_10085"
     CDS             822..2672
                     /locus_tag="VKU83_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HLI93950.1"
                     /translation="MKMLICVAGALALFAGKPEAAEKADTPVLQNIFGRQELNLDGRW
                     KYIIDPYENGFYDYRHHPFDESPSGKGGFYDNRKQDDKSQLIEYDFDRSPSLDVPGDW
                     NSQNAKLLFYEGTVWYRRTFRLHPRQGRRYFLYFAAVNYEAHVYLNGRKLGMHKGGFT
                     PFQFEVTGQLKDGDNFIVVKADNTRHQDDIPTVNTDWWNYGGITRDVVLASVPETYLA
                     DYKVQLAKGDRTRITGYVQLSGPQRSQAAVTVSIAGAKLNWTVRTDSDGLASFNIPVP
                     AGGLTYWSPEEPRLYSVRLSCPTDAITDSIGFRTIEVRGQDLLLNGKKVFLRGIALHD
                     EDPFIPGRNRSAADLRTLLTWAKQLDCNYVRLAHYPHNEKMLRLADQMGILVWAEVPV
                     YWTISWSNPGTYANAAGQLTDLISRDKNRASVIIWSIGNETPLSAPRLSFMSRLAARA
                     HALDDTRLVSAALEVHRKGDEITVDDPLGENLDLVSFNEYAGWYGDDKPGDLARYHFT
                     IKYDRPVLITEFGGGALGGFHADSATRWSEEYQAKLYRCQLEMLGHIGALRGMTPWIL
                     VDFRSPRRQHPVYQNFWNRKGLISGTGQKKEAFFVLKSFYERIAKKYAGS"
     gene            2669..>3300
                     /locus_tag="VKU83_10090"
     CDS             2669..>3300
                     /locus_tag="VKU83_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI93951.1"
                     /translation="MTKGKYFTYVLVLGLLTAIGPFSIDMYLPGFEDIAASLGTTTAM
                     VTLSLSSFFIGISVGQLLYGPLLDRYGRKKPLYAGLGLYLVATFVCMQAKDIETLIIL
                     RFIQALGACAAQVAAMAMVRDLFGPQDSAKVFSLLLLVLGASPMTAPTAGGYIVALWS
                     WRVVFLVLLILGGLIAVLTIFLLPESYPADRQFSLRPLPILRNFLAVLRVR"
BASE COUNT          699 a          867 c         1022 g          712 t
ORIGIN      
        1 gtcccaaggc ctacgaggcg gaggcgatcc gggacaggaa ggtaaaggtc ctgaagcagg
       61 tgaggatgct gacgacacag caggtcttca ggaatgtggt ccgggcgcag tatacggagg
      121 gcgagatcaa tggagaacac cggccggctt accgccagga ggcccaggtc gcgccggatt
      181 caaagacgga aaccttcgtg gcggccagat tttttatcga cagccagcga tggcagggcg
      241 taccgttttt cctgcggaca ggcaagtcga tgccccggca gtcatctgtg atcatggtcc
      301 agttcaagga tagtcctcac aagatcttcc gcgacgacgt ggtgccgaac cgcctggtga
      361 tcagtattca gccggaactc cagattggcc tggtcttcga aagcaaggtg ccgggactgc
      421 atatgaagct gattccggtg gaaatggatt ttacctatag ggagaattat tcgcagtcgt
      481 tgcccgaggc atacgagacc ttgctgctgg acgtgctgat gggagaggcg acctctttca
      541 tgcgaaccga tcagatcgaa gccgcgtgga agcttgtaat gcccatcctg aacgcctgga
      601 agaagtatcc ggggaagcaa ctgcagttct acccggcagg aacctggggg cccgccgctg
      661 cggacaagct gctaaagcct tttgcaaaag actggttcaa gctgactgcg gccccgaggg
      721 tggcccagaa tatggcgagc atcgaataag gcgggccgca gtgcaagttg gcggcggcat
      781 gggaaggacg gacatgcggg atacctcata aaattgcgat catgaaaatg ctgatatgcg
      841 tcgccggtgc gctggctttg ttcgcaggca aaccggaggc agcggaaaag gccgataccc
      901 cggtgttaca aaacattttt ggaagacagg agctgaatct cgacgggcgt tggaagtaca
      961 ttatcgatcc gtacgagaac ggcttttatg actatcgcca tcatcctttc gatgaatcgc
     1021 cttcgggcaa gggcggcttt tatgacaacc ggaaacagga tgataaatcg cagctcatcg
     1081 aatatgattt tgaccgctcg ccttcgttgg acgttccggg cgactggaac tctcagaatg
     1141 cgaagctgct cttctacgaa gggaccgttt ggtaccgccg gacctttcgg ctccatcccc
     1201 ggcaggggcg acgttatttc ctgtattttg ccgcggtcaa ctacgaggcc catgtgtatc
     1261 tgaacggaag gaaactgggc atgcacaaag gggggttcac tccctttcaa tttgaagtaa
     1321 ccggtcagtt gaaagacgga gacaatttta tcgtggttaa ggcggacaac acccggcatc
     1381 aggacgacat cccgacggtc aataccgact ggtggaacta cggtgggatt acaagagatg
     1441 tagtcctggc ttcggtgccg gagacctacc tggccgacta taaggtccag ctcgcgaagg
     1501 gtgaccggac ccggattacc ggctatgtac agttgtccgg gccacagcgg tcgcaggcag
     1561 cggtgacggt aagcattgcc ggtgcgaagc tgaattggac ggtcaggacc gacagcgacg
     1621 gcctggctag tttcaatatt cccgtcccag ccggcgggct gacctactgg tcgccggaag
     1681 aaccgcggct gtattcggtc cgattgtcct gcccgacgga cgcgatcact gacagcatcg
     1741 gcttccggac aatagaggtc cgcggccagg atctcctgct caacggcaaa aaggtgtttc
     1801 tccgcgggat tgccctgcac gacgaggatc cgttcatccc gggccgtaac cggtcggcgg
     1861 cggatttgcg gacgctgctg acctgggcaa aacagctcga ttgcaattat gtccggctgg
     1921 cgcattaccc tcacaatgaa aagatgttgc gcctggctga ccagatgggc atcctcgtgt
     1981 gggccgaggt gccggtctat tggaccatct cctggagcaa ccccggcacc tatgcgaatg
     2041 cggccgggca gttaacggac ctgatcagcc gggataaaaa ccgggcaagc gttatcatct
     2101 ggtccatcgg aaacgagacc ccgttgagcg cgccgcgact gtcgttcatg agccgccttg
     2161 cggccagggc gcacgcgctg gatgataccc ggctggtttc cgcagcgctg gaagtgcatc
     2221 gcaagggcga cgagatcacc gtcgatgatc ccctgggcga aaatctcgat ctcgtaagct
     2281 tcaatgaata tgccggctgg tatggcgatg acaagccggg cgaccttgcg cggtatcatt
     2341 tcaccatcaa gtatgaccgg ccggtcctga tcaccgagtt cggcggaggc gcgctgggcg
     2401 ggttccatgc tgactcggca acccggtgga gtgaggagta tcaggccaaa ttatatcgtt
     2461 gccagctgga aatgctgggg catatcggag cattgcgtgg catgacgccc tggatcctgg
     2521 ttgatttccg gtcgccgcgg cgacagcatc ccgtctatca aaacttttgg aaccggaagg
     2581 gactgatctc cggaacgggg caaaaaaagg aggcgttctt tgtcctgaaa tccttctacg
     2641 aacggatagc gaagaaatat gccgggtcat gacaaagggc aaatacttca cctacgtatt
     2701 ggtgttaggg ctgctgaccg ccatcgggcc cttttccatc gacatgtatc tgccgggatt
     2761 tgaggatatt gccgcaagcc tcgggacgac aacggcgatg gttacgctat ccctgtcaag
     2821 ctttttcatc ggtatttcgg tgggtcaatt gttatacggc ccgctgttgg accggtatgg
     2881 ccggaagaag ccgttgtatg ccgggctcgg cctctatctc gtcgcgacct ttgtgtgcat
     2941 gcaggcgaag gatattgaaa cacttatcat tctgcggttc atacaggcgc tgggagcttg
     3001 cgcggcacag gtggctgcca tggcgatggt gcgggacctt ttcggacccc aggatagcgc
     3061 gaaggtcttt tccctgttgc tgcttgtgct tggtgcatcg ccgatgactg cgcctaccgc
     3121 cggcgggtat atcgtggcgc tgtggagttg gcgggtggta ttcctggtgt tgctgatact
     3181 tggcggattg atcgctgtgc tgaccatctt tttgctaccg gagagttatc cggcggaccg
     3241 gcagttttcg cttagaccgt tgcccatcct gcgcaatttt ctcgctgtgc tgcgggtgcg
//
LOCUS       DATGKE010000593         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1382547, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000593 DATGKE010000000
VERSION     DATGKE010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382547"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VKU83_10095"
     CDS             <1..695
                     /locus_tag="VKU83_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLI93952.1"
                     /translation="GAISTFSTIREGVAQADLVVEAATENTELKLSIFRQLDEFAPAG
                     CILASNTSSISITRIAAATKRPASVIGLHFMNPVPVMKLIEIINGYATDAEVTRQMIA
                     LAGRLDKTPCVVNDYPGFIANRILMPMINEAVYSLYEGVAGVQEIDTVMKLGMAHPMG
                     PLQLADFIGLDVCASILGVLHDGFGNPKYAPCPLLANMVTAGYLGVKSGEGFYRYSSG
                     SKDLVVSSRFTR"
     gene            complement(726..1787)
                     /gene="ribD"
                     /locus_tag="VKU83_10100"
     CDS             complement(726..1787)
                     /gene="ribD"
                     /locus_tag="VKU83_10100"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLI93953.1"
                     /translation="MSRCLELALLGAGHVAPNPMVGAVLLYTDAATGEERIIGEGYHE
                     RYGEKHAEVNCIDAVKEADRPLIEKSTLFVSLEPCVHYGKQPPCADLIIEKRIPRVIA
                     GCRDPFPEVNGKGIEKLRGAGVDVQVGLLERACKELNRRFFTANTLHRPYIILKWAQT
                     ADGKIAGPGRRFISNEYSNRLVHRWRAEESAILVGTATAMLDDPALTVRLWDGVNPVR
                     LVVDKELRLPHSLQLFDGKVRTIVFNRIKHGEAGGVEYYQISPDSSLVHQLSMALAQL
                     KLQSVLVEGGAMLLQSFIDEGYWDEARVITNNELLIPAGLAAPVLAGASLEREECLSG
                     DSIRIYFNRPVPGRGWANG"
     gene            1844..>2603
                     /locus_tag="VKU83_10105"
     CDS             1844..>2603
                     /locus_tag="VKU83_10105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HLI93954.1"
                     /translation="MDLRQAQQELAAAVDALYGSREATLIADWVMEHLTGLKKLDRLM
                     KAGMPLTAEQLKQYQVFRKELLDNRPVQYVLHESWFGGSRFYVDENVLIPRPETEELA
                     GWAASSIAAGPAGPILDVGTGSGCIAIFLALKLPACPVHACDISAGSLRVARRNAIDL
                     QAKVRFHELDFLDSSQWSTLPAMQWMISNPPYIPLREKATLARHVVDAEPALALFVPD
                     GDPLVFYRALAGFAGQQLLPGGAVFVETHEDLAGG"
BASE COUNT          521 a          816 c          707 g          559 t
ORIGIN      
        1 tcggcgcgat cagtactttt tcaaccatcc gggaaggtgt cgcgcaggct gatctcgttg
       61 tggaagcggc aacagagaac accgagctga aattatcgat cttccgccag ctggatgagt
      121 tcgcgccggc gggctgcatc ctggcttcga atacgtcttc tatttcgatc acccgcatcg
      181 ccgcggccac aaaacgccct gcgtccgtca tcgggctcca cttcatgaac cccgttccgg
      241 tcatgaaact cattgagatc atcaacggct acgcaaccga tgccgaagtt acccgccaga
      301 tgatcgcgct ggcaggccgg ctcgataaga ccccttgcgt agtcaacgat taccccggtt
      361 tcatcgcgaa ccgcatcctg atgccgatga tcaatgaggc cgtttacagc ctgtatgaag
      421 gtgtggccgg cgtccaggag atcgacacgg tcatgaagct gggaatggcc cacccgatgg
      481 gcccgctgca actggctgat tttattgggc tggatgtctg cgcttctatc ctcggcgtat
      541 tgcacgacgg gtttggcaat ccgaaatatg caccctgccc gctgctggcc aatatggtaa
      601 ctgccggcta tctcggcgtg aagagcggcg agggctttta ccggtacagc tcagggagca
      661 aggatctcgt cgtcagcagc cgttttaccc gctaatgcag gatcgggcgc catcgaccgg
      721 cgccactacc cgtttgccca tccccgaccc gggaccggcc ggttaaaata aatcctgatg
      781 ctgtcgcccg ataagcattc ttcccgttcc aggcttgctc ccgccaatac tggcgccgcc
      841 agaccagcgg ggatcaacag ctcattgttg gtgatcaccc tcgcctcatc ccaatagccc
      901 tcgtcgataa aggattgcaa caacatggcg ccgccttcca ccagcacgct ttggagcttc
      961 aactgcgcca gcgccatgct cagctggtgc acgaggctgc tgtcgggcga aatttgataa
     1021 tattccacac cccccgcttc gccatgtttt atcctattga atacgatcgt ccgcaccttt
     1081 ccgtcaaata gctgtaagga atgcggcaac cgcaattcct tgtcgacgac caggcggacc
     1141 ggattcacgc cgtcccataa gcgcacggtc agcgcaggat catccagcat agctgtcgcc
     1201 gttccgacga gtatcgccga ttcttcggcc cgccaccggt gcaccagccg gttgctgtat
     1261 tcattgctga tgaatcggcg gcccgggccc gcaatcttcc cgtcggcggt ctgcgcccat
     1321 ttcagaatga tatacggccg gtgcaaggta ttggccgtaa aaaaccgccg gttcaattcc
     1381 ttacaggccc gctccaacaa ccctacctgc acgtcgaccc cggcgccccg gagcttttcg
     1441 atacctttcc cgttgacttc gggaaaagga tcgcggcaac cggcaattac ccgggggatc
     1501 cttttctcaa tgatgaggtc ggcgcaaggc ggctgttttc cataatgcac acaaggttcc
     1561 agggagacaa aaagggtcga cttttcgatc agcggtctgt ccgcttcctt caccgcatcg
     1621 atgcagttga cctctgcatg tttctctcca tatctttcat gatatccttc gccgatgatc
     1681 cgctcctcgc cggtagccgc atccgtgtat agcagcactg ctcctaccat gggattcggc
     1741 gcgacatggc ccgcacccag taacgccagt tcaaggcatc ggctcatata gttttccggt
     1801 gaggccaagg cgcaaatgta agtaaactcg agtattttta cggatggatc tccgccaggc
     1861 acaacaggaa ctagccgccg ctgtggacgc gctttatggc agccgcgaag cgacgctgat
     1921 cgccgactgg gtaatggaac atctcaccgg cctgaagaag ctggaccggc tgatgaaggc
     1981 gggcatgccg ctcactgccg aacagctcaa acaatatcag gtctttcgaa aggaactgct
     2041 tgacaaccgc ccggttcaat acgttctcca tgaaagctgg tttggcggat cgaggttcta
     2101 cgtcgacgag aatgtcctta ttccgcggcc ggaaaccgag gagttggccg gctgggccgc
     2161 cagcagcatc gcggctggtc cggcggggcc cattcttgac gtgggaacgg gaagcggctg
     2221 tatcgccatc ttcctcgccc tgaaacttcc agcctgcccc gtccatgcct gcgatatcag
     2281 tgccgggtcg ctccgggtgg cccgccgcaa cgccatcgat ctgcaggcca aagtccggtt
     2341 ccacgagttg gacttcctgg acagcagcca atggtcgaca ttgcctgcga tgcagtggat
     2401 gatcagcaac ccgccttata tcccgctccg cgaaaaggcc acgctggcgc gccacgtcgt
     2461 ggatgccgaa cctgccctgg ccctgttcgt tcccgacggc gatccgctcg tcttctatcg
     2521 cgcattggcc ggatttgccg gacaacagct attgcccggc ggcgccgttt tcgtggaaac
     2581 gcatgaagat ttggctggcg gcg
//
LOCUS       DATGKE010000594         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1299890, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000594 DATGKE010000000
VERSION     DATGKE010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299890"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VKU83_10110"
     CDS             <1..690
                     /locus_tag="VKU83_10110"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HLI93955.1"
                     /translation="LYVVKFRGAGQGIKALIAECIGGEIARSLGLKVPELVFAGLDAA
                     FGRSEPDEEIQDLLKNSVGLNLGLHYLAGSISFDPVVNPVDPLTASRIVWLDSFLTNV
                     DRTARNTNMLIWNKELWLIDHGAALYFHHSWENWQKHASSPFTPIRDHVLLAAASCLD
                     EVDQDYRTRLPAARIEEIVGLIPDEWLAGGSFPQPPGETRSVYIQYLQTRIAASGIFV
                     KEAQHARHALV"
     gene            668..1051
                     /locus_tag="VKU83_10115"
     CDS             668..1051
                     /locus_tag="VKU83_10115"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HLI93956.1"
                     /translation="MQDTHLFEYAVIRVVPRVDREEFLNVGVILYCPPLRYLDLICTR
                     DYSKLLALDPTIDVGELEKHLDAFGAITRGEAAAGPIGRMDIAGRFRWLTATRSTVLQ
                     TSKVHPGLCGDPRVTIVKLFEDLVL"
     gene            complement(1065..1544)
                     /locus_tag="VKU83_10120"
     CDS             complement(1065..1544)
                     /locus_tag="VKU83_10120"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI93957.1"
                     /translation="MQKIVPFLWYKDNKAEEAANFYVSIIRGAKIIEIMRSRKAGPGP
                     EGSVITVSFTIGDQEIVALNGNPQFDFTMASSLMIHCDTQDEIDEYWDKLSAGGQKIQ
                     CGWLTDKYGVAWQIAPPVLRKMLNDKDPEKAGRVMKAMMQMTKLEIAPLQKAYDGKG"
     gene            complement(1690..1959)
                     /locus_tag="VKU83_10125"
     CDS             complement(1690..1959)
                     /locus_tag="VKU83_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLI93958.1"
                     /translation="MNKAELIDKIAEDAGINKKEANAALDSFTDTIAKTLKKGDKVTL
                     VGFGTFSVSRRAARTGRNPQTGAAIKIKAKKVAKFKAGAILASKV"
BASE COUNT          427 a          568 c          521 g          502 t
ORIGIN      
        1 ctctacgtcg taaaatttcg cggcgccggc cagggcataa aagcattgat tgcggaatgc
       61 ataggcggag agatcgcccg cagcctgggt ctcaaggtac ccgagctcgt cttcgccggg
      121 ctggacgccg cgtttggccg cagcgagccg gacgaggaga tccaggacct gttaaaaaac
      181 agcgtcgggc tcaatctcgg acttcactat ctcgcgggct cgatcagttt cgatccagtc
      241 gtcaatcctg tcgatcccct aacggcgtcc aggatcgtat ggctggactc cttcctgacc
      301 aatgttgaca ggacggcccg taacaccaat atgctgatct ggaacaaaga gctatggctg
      361 atcgaccatg gcgccgcatt atactttcat cattcctggg agaattggca aaagcacgcg
      421 agcagcccct ttacgcccat cagggaccat gtgctcctgg ccgcagcatc gtgtctggac
      481 gaagtggatc aggattaccg cacgaggcta cccgccgcga ggatcgaaga aatcgtaggc
      541 ctgatcccgg atgaatggct cgccggcgga tcattcccgc agccgcccgg ggaaacccgc
      601 agcgtttata ttcaatatct gcaaacccgg atcgccgcat ccggtatctt tgtaaaagaa
      661 gcccagcatg caagacacgc acttgtttga atatgcggtg atccgcgtgg ttccccgcgt
      721 cgaccgcgag gaattcctta acgtgggcgt tatcctgtat tgccccccgc ttcgatatct
      781 cgatctgatt tgtacgcggg actacagtaa actgctcgcg ctcgatccga ccatcgatgt
      841 gggggaactg gaaaaacatc tggatgcatt cggcgccatc acccggggcg aggcggccgc
      901 cggtcctatc ggccgcatgg acattgcagg gcggtttcgc tggctcacgg cgacgcgaag
      961 caccgtcctt caaacctcga aggtacatcc cggcctttgc ggtgaccctc gggtcaccat
     1021 cgtgaagtta ttcgaagatc tcgttttata gccgcggagc aagcctaacc ctttccatca
     1081 tacgctttct gtaacggggc gatctcgagc ttggtcattt gcatcatggc cttcattacc
     1141 ctgcccgctt tctccggatc tttgtcattg agcatttttc gcaggacagg cggagctatc
     1201 tgccacgcga ccccatattt gtccgtcagc cagccgcact ggatcttctg gccgccggca
     1261 gacagcttgt cccaatattc gtcgatctcg tcctgcgtat cgcaatggat catgagcgag
     1321 ctcgccatcg taaaatcgaa ttgcggattg ccgttcagcg cgacgatctc ctggtcgccg
     1381 atcgtgaagc ttacggtgat caccgatccc tcagggccgg gccccgcttt gcgcgaccgc
     1441 atgatttcga ttattttggc tccccggatg atgctcacat aaaaattcgc ggcttcctcc
     1501 gccttgttat ccttgtacca aaggaatgga acgatctttt gcataagctg gtatttttat
     1561 tgttagcaca aacctacttt taaaatgccg tatccagcgg gtggatctcc gacatcctaa
     1621 ggggtggatc gcgaaaaata aaaacccggt cattaaggac cgggccgatc attaaccagg
     1681 aacttactgc tagaccttcg aggcaaggat cgctccagcc ttgaacttgg cgactttctt
     1741 cgctttgatc tttatggcgg cgccggtctg cgggttgcgg cccgtgcggg cagctctcct
     1801 ggaaaccgaa aaagttccaa atccgacaag cgtaacttta tcgccctttt ttaaggtttt
     1861 ggcaatggtg tccgtaaagg aatcaagtgc tgcgttagct tcctttttat tgattccggc
     1921 atcctcagcg atcttgtcaa ttaattcggc tttgttcatc tttgtctata tttatgggtt
     1981 agtgaattgc cgatgaagat acattttttt caattctt
//
LOCUS       DATGKE010000595         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1279186, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000595 DATGKE010000000
VERSION     DATGKE010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279186"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..657)
                     /locus_tag="VKU83_10130"
     CDS             complement(94..657)
                     /locus_tag="VKU83_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HLI93959.1"
                     /translation="MQYPDLTRIDRRWTLFLDRDGVINQEKENSYIFHYGEFVFYDKA
                     KEALRIVDSLFGRIIIVTNQRGVGKGLMTADDLRIIHDKMVAEIVLAGGRIDRIYYAD
                     SLRDDHPDRKPNPGMALAAKAEFPEIDFSRAIMVGNSLSDMEFGKNAGMYTVFLLTTQ
                     AAPTLPHPHIDMVFNSLYDFAKHLQAR"
     gene            complement(639..1790)
                     /locus_tag="VKU83_10135"
     CDS             complement(639..1790)
                     /locus_tag="VKU83_10135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI93960.1"
                     /translation="MKGSVVIIGPGYPLRGGLATFDQRLAREFIAEGYECTIYSFSLQ
                     YPGFLFPGKTQYSTEPPPEGLTILSRINSVNPLNWISAGRELRRQRPDLIVVRYWLPF
                     MGPALGTILRQARKNRHTRIIAITDNVIPHEKRPGDRVFTKYFLAACDAYVTMSEQVL
                     ADLRTFQPTRPARRVVHPLYDNFGEAVTRAQARSWLRETGLPIGDHDKVLLFFGFIRR
                     YKGLDIALQAMADQRIRDLGIKLLVAGEFYEDQAPYRELIDRLNLRDSLLLRTDFIPD
                     SEVRQYVCAADAVIQPYRNATQSGVTPLAYHFEKPMIVSNVGGLAALVPHGQVGLVAE
                     PEPKAMADAILRFFVLGESFFIPALRSEKTKYSWSRLVATIIDLCNTPT"
     gene            complement(1787..2740)
                     /locus_tag="VKU83_10140"
     CDS             complement(1787..2740)
                     /locus_tag="VKU83_10140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI93961.1"
                     /translation="MDISIVIPVYNEAESLPELCSWIERVVRENHLSYEIILVDDGST
                     DHSWEVIQDLRGSNFNVKGVRFQRNYGKSAALNVGFKATKGEVVITMDADLQDSPDEI
                     PSLRKMILEEKYDMVSGWKKVRHDPISKTLPSKFFNWTTARVSKIKLHDFNCGLKAYR
                     SKVVKSIEVYGEMHRYIPVIAKWAGFRKIGEKAVQHRARKYGYTKFGGLGRGLKGLLD
                     LASITFVGKYGKRPMHFFGPLGVICGLIGLGETISLVVQKLDKMNSFSLTNRPSFYIA
                     LTCVIVGTQLFLTGFVAELISRNSPGRNSYLIEQKLGLDPS"
     gene            complement(2757..>3003)
                     /locus_tag="VKU83_10145"
     CDS             complement(2757..>3003)
                     /locus_tag="VKU83_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93962.1"
                     /translation="IDPAFYQRLVPVLLANTESTYRHMGATDDQLRPMLEDIRNNNQF
                     SLGRVISGTGYQLVLFFIIAVLIAATLKSRKGPNPAI"
BASE COUNT          662 a          801 c          878 g          662 t
ORIGIN      
        1 ggagctggta cttaaatgat aacatatcgg tacaaagtta cagattggga aagcgggccg
       61 gaatggccaa acgttaaagg cggccgaaaa atttcaccgc gcctgcaaat gtttcgcaaa
      121 atcgtacagg gagttgaata ccatgtcaat atgcggatgg ggcagggtcg gggcggcctg
      181 agtggtcagc agaaagaccg tgtacatccc tgcgttcttt ccaaactcca tatcgctcag
      241 gctgttgcct accatgatgg cgcggctgaa atcgatttcc gggaattccg cttttgccgc
      301 caatgccatc ccgggattgg gtttcctgtc cggatggtcg tcccggagtg aatccgcgta
      361 atagatgcgg tcgatccgac cgcctgccag cacgatttcc gcaaccatct tgtcatggat
      421 gatccggagg tcgtccgcgg tcatcaggcc ttttcctacg ccgcgctggt tggtgacgat
      481 aatgatgcgg ccgaaaaggg aatcgacaat gcgcaaggcc tccttcgcct tatcataaaa
      541 aacgaattca ccataatgaa agatatagct gttttccttc tcctggttga tcactccgtc
      601 gcggtcaagg aagagggtcc aacggcggtc gattcgggtc aggtcggggt attgcataaa
      661 tcgataatag tggctaccag ccggctccag gaatattttg tcttttcgct gcgaagcgcg
      721 ggaataaaaa aggactcacc gagcacaaag aagcgaagga tcgcgtcggc catggccttc
      781 ggttccggct ctgcaacgag cccgacctgg ccatgcggca ccagtgcggc gaggccccca
      841 acattggaca cgatcatggg cttctcgaaa tgataagcca gtggggtgac gccgctttgg
      901 gttgcgtttc ggtagggttg gatcacggcg tctgccgcgc agacgtactg acggacctcg
      961 ctgtcgggga tgaaatcggt cctcaataaa agcgagtcgc ggaggttcag cctgtcaatc
     1021 agttcgcggt agggagcctg gtcctcgtag aactcacccg ccacgagcaa tttaatgccg
     1081 aggtcgcgga tgcgctggtc cgccatagcc tgcagcgcga tgtccaggcc tttataccgc
     1141 cggatgaagc caaaaaaaag caggaccttg tcatggtcgc cgataggcag gcctgtttcc
     1201 cggagccagc tgcgggcctg ggcccgcgtc accgcttcgc caaaattatc gtaaagggga
     1261 tgtacgacgc ggcgggccgg acgggtcggc tggaaggttc gcaggtccgc caggacctgt
     1321 tcactcatgg taacgtaagc gtcacaggcg gcaagaaagt actttgtgaa aacccggtca
     1381 ccgggcctct tctcgtgcgg gatgacatta tccgtaatgg ctatgatccg ggtgtgccgg
     1441 ttcttccggg cttgtctcag gatcgtgcca agggctgggc ccatgaaagg aagccagtag
     1501 cgcacgacaa tcaaatccgg ccgctgccgc cgcaactcgc ggcctgcgct gatccaattc
     1561 agcggattga cagaattgat gcgcgagagg atagtcaatc cttcgggcgg aggttcggtg
     1621 gaatactgcg ttttgccggg aaaaaggaag ccggggtact ggagagagaa ggaataaatc
     1681 gtgcattcgt aaccttcggc gatgaactcg cgggccaggc gctggtcgaa ggttgcaagg
     1741 ccgccccgga gcgggtaccc gggcccaatg atgacgacac tgcccttcat gacgggtcca
     1801 ggccgagttt ttgttctatc aggtaagaat tgcggcccgg cgagttgcgg gagatcagtt
     1861 cggcgacgaa accggtcagg aacagctggg tgccgacaat cacgcaggtc agggcgatat
     1921 agaacgacgg cctgttcgtc aggctgaagg aattcatttt atccagtttc tgaacaacga
     1981 gggaaatggt ttcgcccaaa ccgatgagcc cgcagatcac gccgaggggg ccgaagaaat
     2041 gcatcgggcg tttgccatat tttccgacga aagtaatgga cgccagatca agcagacctt
     2101 tcaagccgcg gccaagaccg ccgaacttcg tatagccgta tttccttgcg cggtgctgca
     2161 ccgctttttc cccgatcttc ctgaaccccg cccatttggc gataaccggg atataacggt
     2221 gcatttcgcc gtagacctcg atacttttca ccactttgct cctgtacgct ttgagcccgc
     2281 aattgaagtc atgcagcttg atcttgctca cgcgggccgt cgtccagttg aagaacttcg
     2341 acggcagggt tttggagatc gggtcatggc ggaccttctt ccagccgctg accatgtcgt
     2401 acttttcctc gaggatcatc ttgcggagcg aaggtatctc atccgggctg tcctgcaggt
     2461 ccgcatccat agtgatgacc acctctccct tggtggcttt aaagccgaca ttcagcgcgg
     2521 cggatttgcc atagtttcgc tgaaaacgga cgccctttac attgaaattg gaaccgcgca
     2581 ggtcctggat cacctcccag gaatggtcgg tgcttccatc gtccaccagg atgatctcat
     2641 acgaaaggtg attttcccgg acgacgcgtt cgatccagct gcacagctcc ggcagggatt
     2701 ccgcttcgtt atagaccggg atgacgattg agatgtccat gtcgtgtcaa attacattaa
     2761 atggccgggt tcggtccctt tcttgatttt aacgttgccg cgatcaagac cgcgatgatg
     2821 aaaaagagga cgagttggta gccggtgccc gatatcaccc tgccgagtga aaattgattg
     2881 ttgttccgga tgtcctccag catcgggcgc agttggtcat ccgttgcgcc catatggcga
     2941 taggtgcttt ccgtattggc cagcaggacg ggcaccaaac gctgataaaa tgccggatcg
     3001 atc
//
LOCUS       DATGKE010000596         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1393131, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000596 DATGKE010000000
VERSION     DATGKE010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393131"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..2039)
                     /gene="menD"
                     /locus_tag="VKU83_10150"
     CDS             complement(408..2039)
                     /gene="menD"
                     /locus_tag="VKU83_10150"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HLI93963.1"
                     /translation="MTEAHWIIDQLVQQGVRHFCVAPGSRNTPLVLAAAEHPKIKVHV
                     HYDERGLGFFALGIGKAGRGPAALIATSGTAVGNLLPSVMEAHHTCTPLILLTADRPA
                     ELRDCSANQASDQIKIFQPFVRWQVDVPPSLNESYFRSIAAQGYFYARQNPPGPVQIN
                     CQYREPFSIPSSTPFPLQQGKKIVMEFPKLVGKPVGSKHSKGIILVGRLPDPNDIHSI
                     LDLAKRIQWPVFADILSNARLFPTYEQIQSFDWILKKGCDLKPEQILHFGDRLTSKKL
                     LEWLKEVKADMTHVSPYPFLQDPARILTGRVQADIPGFCTHFQAPSDPHWLAGWQNLD
                     SEISSLAESRFADSSPFTEAHAMRALARAIPSDFSIFLGNGMPIRDADHFLYPKKLKG
                     FFCNRGLSGIDGNIATAAGIAEGLQAPVAAFIGDQATLHDLNSLPLLKKYPVFLIASN
                     NFGSGIFHHLPIAKSPHFETYWAASHNWHFEMAAKMFDIPYMRLDSDPEEALGAVFQT
                     RSSSLTEMITDRTENYLFQQQIQILMKECYGNQNI"
     gene            complement(2036..>2236)
                     /locus_tag="VKU83_10155"
     CDS             complement(2036..>2236)
                     /locus_tag="VKU83_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HLI93964.1"
                     /translation="RGLYGGAIGWSTQDASEWVVGIRSCFIQGKTATLFSGTGIVQGS
                     DPISEWDELDQKLKLYDGIFVE"
BASE COUNT          540 a          506 c          620 g          570 t
ORIGIN      
        1 gaagcaggtg aacgcagtga actggccggc gtgagtgaag atcaaaaatt tactgcgaag
       61 ctgacgcatt caaaaaccaa attttgagtt catttgtgta tatcgcgatg aaaagggctg
      121 ctgctataga ggaaacaagg agagtatagc tttttttttc tggatacctt ttgtcttgaa
      181 gaaaattggc ttgtcgcatt gcggacttgt aatattctat ctcatgcccc tcatcaatcg
      241 atttattggg ccgtgggata ggcggctgcg ctggcccaaa ccgcgatgcc agtactcgcc
      301 gaacccctga taccggtgct tgccgaaccc atgataccgg tgtggggtca actcgataac
      361 atgcttcgtg agcgtcttta agagctgttt gtaaagggat ttcccgatta tatattttga
      421 ttgccatagc attctttcat taatatttga atttgttgtt gaaaaagata gttttcagtc
      481 cggtcggtga tcatctcggt cagcgaggaa gaacgggttt ggaatacggc gccaagagcc
      541 tcttccggat cggaatcgag ccgcatataa gggatatcga acatcttcgc cgccatttcg
      601 aaatgccaat tgtgagatgc ggcccaatac gtttcgaagt ggggcgattt ggctatggga
      661 agatggtgga agatcccgct gccgaaattg ttcgatgcga tcaggaagac cggatatttt
      721 ttcaataggg gcaaggaatt gaggtcgtga agggttgcct ggtcgccgat gaatgcggcg
      781 acaggcgcct gtagtccttc ggcaattccg gccgcggttg cgatattgcc gtcgattccg
      841 gataagccgc ggttgcagaa aaagcctttc aattttttgg gataaaggaa atgatccgca
      901 tcgcggatcg gcattccgtt tcctaaaaat atagagaaat cagacgggat cgccctcgcg
      961 agggcgcgca ttgcatgcgc ctcggtgaac ggagaactgt cggcaaatcg cgattcggcg
     1021 agggaggaaa tctcagagtc caaattttgc caccctgcta accagtgcgg gtcggagggg
     1081 gcttgaaaat gggtgcaaaa accagggata tcggcctgga ctctgcccgt cagaatgcgg
     1141 gcgggatctt gaagaaaggg ataagggctg acgtgggtca tgtcagcctt tacttcttta
     1201 agccattcga ggagtttttt cgatgtcagc cggtccccga aatggaggat ttgttcgggt
     1261 ttcaaatcgc accctttttt caaaatccag tcgaacgact ggatctgttc gtatgtcggg
     1321 aagagccggg catttgaaag gatatctgcg aaaacgggcc attggatccg ttttgccaaa
     1381 tccaaaatgg aatggatatc gtttggatca ggcagccgtc caaccaaaat aatgcctttt
     1441 gaatgttttg atcctaccgg ttttccaacc aatttcggaa actccatcac gattttcttg
     1501 ccctgctgga gtgggaaagg ggttgaactg ggaatggaaa acggttcgcg gtactggcaa
     1561 ttgatttgca ctggaccggg gggattttgg cgggcataga aatatccctg ggctgcaatg
     1621 gagcggaaat acgattcatt caaacttgga ggtacatcta cctgccaccg tacaaaaggc
     1681 tggaaaattt tgatctggtc cgaggcttgg ttcgcgctgc aatcgcgcag ttccgcaggg
     1741 cggtctgctg tcaagaggat caatggggtg caggtatggt gtgcttccat gacgcttgga
     1801 agaagatttc cgaccgctgt gcccgatgtg gcaatgagcg cagccgggcc tcggcctgct
     1861 ttcccgatgc ctaaagcaaa aaatccaagc ccccgttcat cgtaatggac atggacttta
     1921 atctttggat gctcggcagc ggccaataca agcggcgtgt tgcgcgatcc gggggccacg
     1981 cagaaatgcc gtactccttg ttggactaat tgatcgatga tccaatgggc ttcggtcatt
     2041 ctacaaatat cccgtcgtag agtttgagtt tttgatccag ttcatcccac tctgagattg
     2101 ggtcggatcc ttgcacgatt cctgtccccg aaaacaaagt ggcagttttc ccttgtatga
     2161 agcaggaacg gatccctacg acccattcgg aggcatcctg agtggaccag ccgatcgccc
     2221 ctccataaag acctct
//
LOCUS       DATGKE010000597         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_389683, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000597 DATGKE010000000
VERSION     DATGKE010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389683"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..970)
                     /locus_tag="VKU83_10160"
     CDS             complement(155..970)
                     /locus_tag="VKU83_10160"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLI93965.1"
                     /translation="MPVISIAIQKGGSGKTTTALNLGAALSQQGKKVLLIDADPQANL
                     SQSLGIEEEPRFNLYSELKKEILQEGGRLSDAIVSTATGIDVIPASIDLAIVELEMAG
                     LIGREYLINDLLKPLTDKYDYIFIDCPHAISLLTVNALVASDYVMLPLPGEFLPLKGV
                     YGFMRQFDIIRKKLNSRLELMGMVMTKYDERKQMNVGVRRQLEEKFNGKVFQTVIRTN
                     IQLAKAQEAGKDIFSFDRGSNGAKDYRQLAGELVKRSYNGPVEIGAAMEAAIG"
     gene            1134..1892
                     /locus_tag="VKU83_10165"
     CDS             1134..1892
                     /locus_tag="VKU83_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HLI93966.1"
                     /translation="MKRSFALLLCVTAAGLAQAQPSRFSREKYIEKGDTLLYRMLFPD
                     ANRLRKYPLVIFLHGSGERGSDNDAQLKWGVMNFATDQNMLAHPAIVIAPQCPERQNW
                     SSVTPNKTRTGITLNVEPSTPMRLLIALIRQLETTMPVDTNRIYITGLSMGGYGTFDA
                     LERYPNLFAAAVPVCGAGDTSRAASIAHVPTWIYLGAEDPAVNPLLGLAMFEALTNAG
                     AHPGFTQYPEVGHFSWLGAYSDPLMMEWLFRQHK"
     gene            2231..2644
                     /gene="yiaA"
                     /locus_tag="VKU83_10170"
     CDS             2231..2644
                     /gene="yiaA"
                     /locus_tag="VKU83_10170"
                     /inference="COORDINATES: protein motif:HMM:NF008482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HLI93967.1"
                     /translation="MSTKPSNAFIGASWIALGTGTLGYLIGLYRAQMQLNEKGYYFTV
                     LMFALFAVVSLQKSVRDRLEGIPVTDLYYGISWFATILSIILLTIGLWNATILPSEKG
                     FYAFSFLLAIFGAVAVQKNTRDTLQAEKSDKKDPS"
BASE COUNT          650 a          768 c          686 g          683 t
ORIGIN      
        1 gctccatcga gaccttcttc agagaaatga gcaaatttgg cccgtccatc cccaagggcc
       61 aggaccaggc gctccggtcc ctctttcgta cccacggcat ggaacttctc ggccccccgc
      121 ttcagatctg aaccgtgcga acaaacaatc caaatcaacc gatcgctgct tccatcgccg
      181 ctccgatttc gacgggtcca ttgtaggacc tcttcaccag ttcccccgca agctgccggt
      241 aatccttggc gccgtttgag ccacggtcga agctaaagat atccttaccc gcttcctgcg
      301 ctttggccaa ttggatattc gtgcggatta ccgtctgaaa taccttgccg ttgaacttct
      361 cctccagctg tcttcttaca ccaacattca tttgtttgcg ctcgtcatat ttcgtcatga
      421 ccatgcccat cagctccagc cgggaattga gtttctttcg aatgatatcg aattgccgca
      481 tgaacccata cacgcccttc agcggcagga actcgcctgg cagcggcagc atcacgtaat
      541 cgctggccac cagcgcattc acggtaagta ggctaatggc atgcgggcaa tctataaaaa
      601 tgtagtcgta tttatcggtc agtggcttca acagatcatt gatcaggtat tccctgccta
      661 taaggccagc catctccagc tcaacgatag ccagatcgat cgaagcaggg atcacatcga
      721 tccccgtagc cgtagagacg atggcgtccg acaaccggcc cccttcctgc aggatctcct
      781 tttttaattc ggaatacaga ttgaatcggg gttcttcctc gatgccgagc gactgcgata
      841 gattggcctg cggatcggca tcgatcaata atactttttt accttgctgg gataaggcgg
      901 ctcctaaatt aagcgcggtc gtggtctttc cggacccgcc tttttggatc gcgatcgaaa
      961 taactggcat gttctactgt tttaaggatt agattctcta aaaataggca tagttggtct
     1021 tatgactgta attaagttcc taacggttgt ggattatgcg cggttgggtc accactgcat
     1081 tcacccgaca ttttttgact tggcatgtca aaaaataacg taaattaaat gccatgaaac
     1141 gatcgttcgc tctcctcctg tgcgtcacgg ccgcaggcct ggcgcaggcc cagccctcac
     1201 ggttcagccg cgaaaagtat attgaaaaag gcgatacgct gctctatcgc atgttgttcc
     1261 ccgatgccaa ccggctaaga aaatatccgt tggtgatctt tctgcatggg tcgggagaac
     1321 ggggcagcga caacgacgca cagttgaaat ggggcgtcat gaatttcgcc accgaccaga
     1381 atatgctggc gcatccggcg atcgtgatcg cccctcagtg ccccgagcgc caaaactgga
     1441 gcagcgtaac ccccaacaag accaggaccg gcataacgct gaacgtcgag ccgagcacgc
     1501 ccatgcgctt gctcatagcg cttatccgcc agctggagac gacgatgccg gtcgatacca
     1561 accgcatata tatcaccggg ctgtcgatgg gcgggtacgg aaccttcgat gcgttggagc
     1621 gataccctaa cctgtttgcc gcggccgtac cggtttgcgg ggccggcgac acgtcgcggg
     1681 cggcgtcgat cgcgcatgtc ccgacgtgga tctacctggg ggcagaggac ccggcggtca
     1741 accctttgct cgggctggct atgttcgaag cattgaccaa cgccggcgca catccgggtt
     1801 ttacccaata tcccgaggtc ggccattttt cctggctggg ggcctacagc gatccgttga
     1861 tgatggagtg gttgttccgg cagcataaat aaagccgtcg ccaggtctga tcatcccgcg
     1921 tggctcactc cgcggcagat cacagacgcc tgggcggcat tcctgcctgg attcgacagc
     1981 acccaccacg aaattttcga tatcgacgct agcgatagca aggatacctc cgttcttcat
     2041 tttaaaggga gagcggagca ttttatcgga aaagagtcga tcgcacaatt caaattcaat
     2101 ttgcaggccc aaagcgggaa tgtacagttg ccggcagtag ctgccgaaaa gatgcggaat
     2161 gatccggata accgtcgatc ttaaaaaaat ctgtaccttg atagatcaat aacaaaactc
     2221 ctaacaccgt atgtccacga aaccatccaa tgcctttatc ggcgcatcct ggattgcctt
     2281 gggtacagga accctgggtt atctgatcgg cctatatcgc gctcaaatgc agctcaacga
     2341 aaaagggtat tactttacgg tattgatgtt cgccctgttt gccgtagttt ctctgcaaaa
     2401 aagcgtgcgg gatagactgg aaggaattcc cgtgacagat ctgtattatg ggattagctg
     2461 gtttgcaact atcctttcca ttatcttgct gaccatcggt ttgtggaatg caaccatcct
     2521 gcccagcgag aagggattct acgcattttc ctttttgctg gcaatttttg gcgccgtggc
     2581 ggttcagaag aatacccggg ataccttgca ggcggaaaag tcggacaaga aagacccttc
     2641 ttaagccttc ccaaaagaaa aatagctgat tcgatgtgta atcagggatt ttcaagcatc
     2701 ctattgaaaa gccctgttta tgagatcttt ctacatcgcg cttgtattcc tgcttttcag
     2761 ccgtgagctt tccttctcac aacccat
//
LOCUS       DATGKE010000598         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1408385, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000598 DATGKE010000000
VERSION     DATGKE010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408385"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VKU83_10175"
     CDS             complement(<1..1288)
                     /locus_tag="VKU83_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93968.1"
                     /translation="MRKNRLTTFLFCVLVWCGAEAQALRPGTPKWIVGTGEYQCYFIE
                     TSTGKLYGLSAAAGAVGAEDGVPETPMPVAVPSDRRTVSVASGLHHSVSADDHGDVWA
                     WGANDQGQEGRGEIGGYYALPKQIKVDSLGNPFTGVVQVVCWANYKGAGSGAVKSDGT
                     VWVWGNTGGGLRGGGQEGQNNTRPVQVVMPQGVKVVKLVVGENAMALASDGTVWTWGG
                     YGRTEYLGTGSADYQHPHRVPIPQPVRDISGNGMFNYALTNKGTLFGWGLYSSYMGIG
                     KGGFMASNFFVRMPRDLTQDLRLPHRIMMISTNSVSTHVILTDSTLWGWGDNAMGCVG
                     NGHELDYATYKMPYQWDWAPGQLLQQSPVRLMPKIHNFVQVFGATAAIFYCYAETATG
                     QLYSWGRNKAGVLGNGIMSAAPEIEAKYPNSWDVTTV"
     gene            complement(1379..>2131)
                     /locus_tag="VKU83_10180"
     CDS             complement(1379..>2131)
                     /locus_tag="VKU83_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI93969.1"
                     /translation="GTIISYSWVQLSGSGGVTISGSTQAQATVSGLIAGTYVFQLTVT
                     DDAGASGVATVTITVNAAVVQPPVANAGNDTTIAFPASSVMLNGTGSSDPAGEPLTYS
                     WYELSGPAEVTIAAPDAIQPTATGLQPGVYVFGLQVINTAGLTDTASVEVRVVNSQRT
                     TAADTGSAQVSVYPNPVQDVLTVKFTDPGVSGNVLLTVFDMRGRRLISEQVQVVSGAQ
                     LLTLNVAGLARGTYALEVVTGRAKSNQLIVKQ"
BASE COUNT          406 a          737 c          552 g          436 t
ORIGIN      
        1 tcaccgtagt cacatcccag gagttgggat atttcgcttc gatctccggc gctgcgctca
       61 tgatgccgtt gccgagcacg cctgccttgt tcctgcccca ggaatacaat tgacccgtgg
      121 ccgtctcggc ataacagtaa aatatagccg cagtagcgcc gaagacctgg acaaagttgt
      181 ggatcttcgg catcagccgg acgggggact gctgtagcag ctggccgggc gcccagtccc
      241 attgatacgg catcttgtac gtcgcgtaat ccagttcgtg cccgtttccc acacaaccca
      301 tcgcgttgtc gccccagccc caaagcgtgc tgtctgtcag gatcacatgg gtactcacac
      361 tgtttgtgga aatcatcatg atccggtggg gcaaccggag gtcctgcgtc aggtccctcg
      421 gcatccggac gaaaaaattg ctggccataa atccgccctt tccaatgccc atgtaagaag
      481 aatataatcc ccagccgaag agcgttcctt tattcgtcag cgcatagttg aacatcccgt
      541 tccccgaaat atccctgacg ggctgcggaa tcggcacgcg atggggatgc tggtaatccg
      601 ccgagcctgt ccccaggtat tcggtccgcc cgtatccgcc ccatgtccac acggtcccat
      661 cgctcgccag cgccatcgca ttttccccga cgacgagctt cacgaccttt accccttgcg
      721 gcatcacgac ctgtaccggc cgtgtgttgt tctgtccttc ctggccgccg cccctcaacc
      781 cgccgcccgt attgccccag acccaaacgg ttccgtcgga cttcaccgcc ccgctgccgg
      841 cgcccttgta attcgcccag cacactacct gcacgacgcc ggtgaatgga ttcccgaggc
      901 tgtcaacctt gatctgcttc ggcaatgcat aatatccgcc gatttctccc ctgccttcct
      961 gcccttgatc atttgctccc catgcccaaa cgtcgccatg gtcatccgct gacacactat
     1021 ggtgcagacc cgacgctacc gataccgtcc ggcggtccga aggaacggct accggcatcg
     1081 gcgtctcggg cacgccgtcc tctgcaccga ccgcaccggc ggcggcgctg agcccgtaca
     1141 atttcccggt gctggtttca ataaaataac attgatactc tccggtgccg acgatccatt
     1201 ttggagtacc cggccgtaaa gcctgcgcct ccgccccgca ccagacaagt acacaaaaca
     1261 ggaaggtggt caaccgattt ttacgcattg cataaatata cgcaatcacg ggcactcatc
     1321 tccccgtgca cgccgcgccc ggcgccggac tccctgccgc cgttcccgca gcccatccct
     1381 attgcttcac gatcagctga ttacttttcg cgcgacccgt cactacctcc aacgcatacg
     1441 tccccctggc caggccggca acattcaatg tcagcaactg cgcgccgctg acgacctgaa
     1501 cctgctctga tatcaaccgg cgtccgcgca tgtcgaagac cgttagaagc acgttcccgc
     1561 tgacacccgg gtcggtgaat ttcaccgtga ggacgtcctg caccggattc gggtagacgc
     1621 tcacctgtgc gctgccggta tctgccgctg tcgtccgctg gctgtttacg acgcggacct
     1681 ccacggaggc ggtgtctgtc aggccggcag tgttgatgac ctgcagcccg aagacataga
     1741 cacccggctg caggccggtt gcggtcggct gtatcgcgtc cggggcggct atcgtcacct
     1801 ccgccggccc gctcagctcg taccaggaat aagtcagggg ctccccggcc ggatcgctcg
     1861 agccggtgcc attcaacatc accgaacttg ccgggaaggc aattgtggta tcattgccag
     1921 catttgccac cggcggctga acaaccgcgg cattcaccgt aatggtcacg gtcgcgacgc
     1981 ccgatgctcc cgcatcgtcg gtaaccgtca gctggaaaac ataagtaccc gcgatcaatc
     2041 ccgaaacggt tgcctgggcc tgcgtcgatc cgctgatcgt cacaccgccg gatcccgaga
     2101 gctgcaccca gctgtaggag atgatggtcc c
//
LOCUS       DATGKE010000599         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_657091, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000599 DATGKE010000000
VERSION     DATGKE010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657091"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VKU83_10185"
     CDS             <1..724
                     /locus_tag="VKU83_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93970.1"
                     /translation="VLPPSRVSLASNMQPGPLGEYAASTHLQGELLADCLDILIRDAT
                     DSARSLDDVMRRIYQRFGNHQVLHDSDIEAAATGVCQCDAVHAFFQDNIYAGKPIDFN
                     RWLRILGLSVQHDQVPAAGMDGRPRPDTRVYSWFLRDDPSLRIGISNPNSCWALAGLR
                     TGDVIAAINGSPMRTRKDFQDAIAGLHVGDSLVVQTGKGAELTRLIIPITGYTTPVIH
                     ISQDTGATAKQQRLLRLWLAAK"
     gene            complement(1291..>4188)
                     /locus_tag="VKU83_10190"
     CDS             complement(1291..>4188)
                     /locus_tag="VKU83_10190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLI93971.1"
                     /translation="QRKDGAEMQAKYEWRTDSSAGYTYRYVTNDPLQTRVYTLKNGLT
                     VLLSANHKEPRVTVRIAVRAGSNTDPRDHTGLAHYLEHLLFKGTDQYGTLDWAKEKPY
                     LDKIDALYEQYNSTRDPLQRKAIYHRIDSVSGLASHYSIASEYDKLMADIGAQGSNAH
                     TWKEETVYEEDIPSNAMDKFLAVQAERFRYPVFRLFHTELEAVYEEKNRSLDNDGAKM
                     NEAMHAALFPTTNYGQQTTIGTIQDLKNPSLKAIRAYYHKYYVPNNMAVIMAGDFDPD
                     ALIRKIDEAFAYMKPQPVEGYQHAPERDLTQPVVKTIYGPSAESIRIAFRTPAADTRD
                     AVTLDLIGSILSNDKAGMLDLNLNKQQKVQGAEAEFGQYKDYGVLVFAASPRQGQTLD
                     QVKDLILQQIQLLKKGQFDDSLVKAIVANGKLAELEGLQKNEPRAVALMTAFIQNKAL
                     KWDQDVAELDEQATIGKPEIVAVANKYLKNNYVLIYKRKGEDKTIVKVDKPPITPVET
                     NAGKQSDFVKRIEAMPVAPVQPQWLNFDSGIVHAKAGIANVLYVPNKENDLFHLYYRF
                     GIGSWNDRLLPMAAQYLQYLGTPEHPTDEIARAFYSIACNFSINTTTEETTISITGLQ
                     ENFGRAVALFEDVMRHCQPDSGALVALKGRLLKARANNKLNKQAILQGLINYARYGAR
                     NPFNSGLTTEELRVMKPEDLTKVLHSLMDYQHTIIYYGPLPVNAFTSAITGVHPLPAK
                     WTPDPPKVRFPFAEQTANKVLFADYDMVQAEICWIRNSERYDVKKEPVVDLFDQYFGG
                     SMGSVVFQTLRESKALAYSTFAGYETPAKQADPFYVIAYIGCQADKLNEAIDGMNGLL
                     NDLPVSEKSFDLAKTSEKKDIETARYTGDEIVFAYIGETEKGLDYDERKEEYAALDTL
                     TMDDVKRFHNQELANKAYTYCVVASQKKIKLDDLKKHGDVKVLSLEEIFGY"
BASE COUNT          823 a         1221 c         1099 g         1045 t
ORIGIN      
        1 cgtccttccg ccctcgcgcg taagtctcgc gtccaatatg caacccggtc cccttggcga
       61 atacgctgca agcacccatt tgcagggcga gcttctcgct gactgtctgg acatcctgat
      121 ccgcgatgcg accgatagcg cgcggtcgct cgatgacgtc atgagacgga tctaccagcg
      181 cttcgggaac caccaggtct tgcacgacag cgacatcgaa gcggcagcga ccggcgtttg
      241 tcaatgtgac gcggtgcatg ccttctttca ggacaatatt tacgccggca aacccatcga
      301 tttcaaccgc tggctccgca tccttggtct tagcgtccaa cacgatcagg tcccggccgc
      361 cggcatggac ggacgccccc gtcccgatac ccgggtctat agctggtttt tgcgcgatga
      421 cccgagcttg cgtatcggca ttagcaatcc caatagttgc tgggcgctgg cagggctgcg
      481 taccggcgat gtcatcgcgg caatcaacgg cagcccgatg cgaacgcgca aggacttcca
      541 ggatgccatc gccggccttc atgttggcga cagcctggta gtccaaacgg gaaagggggc
      601 ggagttaacc cgattgatta ttcctatcac cggctacacg acgccagtca ttcatattag
      661 ccaggacacc ggcgcgacgg ccaaacagca gcgcctgctc agactttggc ttgcggccaa
      721 atgagagtca gcttcgctat gttcacgcat cgccgcggtc agattattgc ggaacatacg
      781 ttggacgttt ttttgaaaaa tgggcgaaga aaatgccagt tttgcaccca attcagcttc
      841 aaccttttag cataaatgat cccccgcgcg ctgttcgatt tcgcaacatc gcctgtccgc
      901 tcatcaaaaa atttcattat tttcgttgcg ctaaaaccaa tatcaaatgc ggcttcagaa
      961 agcgcttatc tatctgatta tctcaacttt ctttcttctc ggggtctatg aaatcaaaag
     1021 cggcaacgtc cgcgacttcg cttactacaa aaatgcaact gatcctgtgt ctgtcacggt
     1081 acccggcaac tggctggtga tttgtgccgt cgggctcttt atagtaatta gttatacttt
     1141 ccataaaata aagaattgac gacccctggt cccttttttc ccctcgccca accctgctta
     1201 cttcttccaa cccctaagcc ctaaacctaa acccttccgg aggccaatgg ccggctaaac
     1261 cccggccttg caccggacgg gcacgccccg ctagtatcca aagatctctt ccagcgaaag
     1321 caccttcaca tccccgtgct ttttcaggtc gtccagcttg atcttcttct gtgatgccac
     1381 cacgcaatag gtatatgcct tgttggcgag ttcctgatta tgaaaccgct tgacgtcgtc
     1441 catggtcagc gtatccaacg ctgcatattc ctctttccgc tcatcataat caagcccctt
     1501 ctccgtttca ccaatgtacg cgaacacaat ctcatcgccc gtgtacctgg ctgtctcgat
     1561 atccttcttc tcgctggtct ttgccagatc aaaacttttt tccgacaccg gcaggtcgtt
     1621 caacagcccg ttcatcccgt caatcgcctc attgagcttg tcggcctggc agccgatata
     1681 cgcgataacg taaaagggat ccgcctgctt cgccggcgtc tcatagccgg caaacgtcga
     1741 ataagccagc gccttcgact ccctcaacgt ctggaagacg accgaaccca tgctcccgcc
     1801 aaagtactgg tcgaacagat cgaccaccgg ttccttcttc acgtcatacc tctcagaatt
     1861 gcggatccag cagatctccg cctggaccat gtcgtagtca gcgaaaagca ctttgttggc
     1921 tgtctgctcc gcaaacggaa acctgacctt cggcggatcc ggcgtccact tcgccggcaa
     1981 cggatgaaca ccggtgatgg cggacgtaaa ggcattcacc gggagcggcc cgtaatagat
     2041 gatcgtatgc tgatagtcca ttaatgagtg cagcactttg gtaaggtcct ccggcttcat
     2101 gacgcggagc tcctccgtcg tgaggccgct gttgaaaggg ttcctcgcgc cgtaccgcgc
     2161 atagttgatg aggccctgca ggatagcctg tttatttagt ttattattgg cccgggcctt
     2221 tagcagccgg cccttcaggg caaccagcgc tcctgaatcc ggctgacaat gccgcatcac
     2281 atcctcgaat aacgctaccg cccggccgaa gttctcctgg aggcccgtga tggaaatcgt
     2341 cgtctcctcc gtcgtggtgt tgatgctgaa attgcaggca atggaataga aggcccgtgc
     2401 gatctcgtcg gtcggatgct cgggcgttcc caggtattgc aggtattgcg ccgccatcgg
     2461 caggagccgg tcattccaac tgccgatccc gaagcgataa tagaggtgaa agaggtcgtt
     2521 ttccttgttg gggacataga ggacattcgc aattcctgcc ttggcgtgga caatcccgct
     2581 gtcgaaattc agccactgcg gctgcaccgg cgctacgggc atggcctcga tccgcttcac
     2641 aaagtcagac tgtttgcccg cgttcgtttc gacgggcgta atgggcggct tgtccacttt
     2701 gacaatggtt ttgtcttcgc ccttccgttt atagatgagt acataattgt tcttcaggta
     2761 tttgttggca acggcgacga tttccggctt gccgatggtc gcctgttcat ccagctccgc
     2821 gacgtcctgg tcccatttta gcgccttgtt ctggataaag gcggtcatca gggcgactgc
     2881 ccgtggctcg ttcttttgca gcccttccaa ctccgcgagc ttcccgttgg ccacgatcgc
     2941 tttcacgagg ctgtcatcaa attgtccctt tttcagcagt tgtatttgct gcaaaatgag
     3001 atcctttacc tggtcaagcg tctggccctg gcgcggagaa gccgcgaaga ccaggacgcc
     3061 atagtccttg tattggccga attccgcttc tgcgccctgg accttttgct gtttgttgag
     3121 gttaaggtcc agcattccgg ccttgtcatt cgaaaggatg cttccgatca gatcgagggt
     3181 caccgcgtca cgcgtatcgg cggcgggcgt gcgaaatgcg atgcggatgc tctccgcgct
     3241 ggggccatat atcgtcttca cgactggctg cgtcaggtcc cgttcgggcg catgctgata
     3301 accttccacc ggttgcggct tcatataggc gaaggcctcg tcgattttcc tgatcagcgc
     3361 gtccggatca aaatcccccg ccatgatgac cgccatgtta ttcggtacgt agtatttatg
     3421 gtagtaggcc cggatagcct tcagcgaggg gttcttgagg tcctggatcg ttccgatggt
     3481 cgtctgctgg ccgtagttgg tggtcggaaa aagcgctgcg tgcatcgcct cgttcatctt
     3541 ggcgccgtcg ttgtccaggc tgcggttttt ctcttcataa acggcctcca gctccgtgtg
     3601 gaaaagacgg aataccgggt agcggaaacg ctccgcctgc acggcgagga atttgtccat
     3661 cgcgttcgag ggtatgtctt cttcatagac ggtctcttcc ttccaggtat gtgcgttcga
     3721 gccctgggcg ccgatatccg ccatgagctt gtcatactcg ctggcgatgg aataatggga
     3781 ggcaaggccg gagacgctgt cgatgcggtg ataaatcgcc ttgcgctgca gggggtcgcg
     3841 ggtcgaattg tattgttcat agagggcgtc gatcttgtcg agataaggtt tctcttttgc
     3901 ccagtcgagc gtcccatact ggtcggtgcc cttgaaaagg aggtgttcaa gataatgggc
     3961 caggcccgtg tggtcgcggg gatccgtatt gcttcccgcc cgcactgcaa tccggaccgt
     4021 cacgcgcggt tccttgtggt tggcgctaag caggaccgtc aaaccattct tgagggtata
     4081 tacccttgtt tgaagcggat cattggtcac ataccgatag gtataacctg cggagctgtc
     4141 ggttctccat tcgtatttgg cctgcatctc ggcgccatcc ttccgctg
//
LOCUS       DATGKE010000600         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_640702, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000600 DATGKE010000000
VERSION     DATGKE010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640702"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1638)
                     /locus_tag="VKU83_10195"
     CDS             complement(58..1638)
                     /locus_tag="VKU83_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI93972.1"
                     /translation="MRKRSRLSLSLVAFLLVSAGLTAQNEKIDMATIQKIRQEGLQDS
                     HVMDIAFHLTDASGDRLTGSAGFFRAANWAKEELAQWGLENATLDPWGEFGKGWELEK
                     SYVALSAPYYKPLIAFPKAWCAGTNGPQTAQVVLIEAKDSTDLDQYKGKLAGKVLIMD
                     SHEVYEQSFKPDASRWTDAQLDSMAHVVFSPPDTAAIRRRREAFLRGGGQRLVNALKK
                     MAEEEGAIAILSQSARGHDGTLFVQGGGAYRADAPPNFLDIEIALEDYMTIVRLCKAG
                     TPVSLDVDVKTKFDTNDTKGYNVIAEIKGTDPQLKDQIVMLGGHLDSWHSGTGATDNA
                     AGCTVMMEVVRILKTLGIKPRRTIRIALWSGEEEGLLGSRGYVKKTFADPADMKLLPE
                     HSKLSGYFNIDNGTGKVRGIYLQGNEACRPIFTQWLEPFRDLGATTVTISNTGGTDHQ
                     SFDAVGLPGFQFIQDEIEYSTRTHHTNMDTYDHLRADDLKQIATVVAGFVYNAAQRNE
                     MLPRKPLPQPRGPGRGAF"
     gene            1740..2270
                     /locus_tag="VKU83_10200"
     CDS             1740..2270
                     /locus_tag="VKU83_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLI93973.1"
                     /translation="MRKLRMDELDRKSVNDFRQAEKIPIVVVLDNIRSMHNVGSVFRT
                     ADAFLIRAIYLCGYTPVPPHRDIHKTALGATETVAWRHFAAVTDAIAGLKDEGYAVVA
                     VEQTEKSVALSAIRDRFPGPLAVVFGNEVGGVTEQALALCDGSVEIPQWGMKHSLNVS
                     VAAGIVLWESVRQRTL"
     gene            2278..3210
                     /locus_tag="VKU83_10205"
     CDS             2278..3210
                     /locus_tag="VKU83_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HLI93974.1"
                     /translation="MSTFKSYLSLIRFSHTIFAMPFAMIGFFLGVAELTYWDRTGAIA
                     SGGQGWHVTDWRMDYFLFKFVLVILCMVFARSAAMAFNRWLDVKFDALNPRTAIREIP
                     AGIISKNSALRFVAVNCVLFILSTWFINPLCFLLSFVALAVVLGYSYTKRFTPLCHLV
                     LGLGLSLAPIGAYLAVAGRFAWLPILFSLTVIFWVGGFDIIYALQDEEFDRTQQLYSM
                     PSWLGKAKALRVSEILHLLSTACVVTAGIYGGFGCWYWAGVAVFIGMLIYQHSIVKPG
                     DLSRVNLAFMTANGIASVVFAGLVIADLYWYAHI"
     gene            3245..>3547
                     /locus_tag="VKU83_10210"
     CDS             3245..>3547
                     /locus_tag="VKU83_10210"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HLI93975.1"
                     /translation="MRQRMKERLNLTIDGALLAAMKVYASSKGISISELVESYFRAVT
                     KPLNRKNILHLVEELDPPGISVDQAAGGKKPLAEPEAELLSPARQPEPLTKFKRGRH"
BASE COUNT          722 a          893 c         1031 g          901 t
ORIGIN      
        1 taagaaagcc cctacaattc ggggatcttt cctacggggt aaactggcgg cgggtcatca
       61 gaacgccccg cggccagggc ctctcggctg cggcagcggt ttgcggggca gcatctcgtt
      121 gcgttgcgcc gcattgtaca caaaaccggc gacgacggtg gcgatctgtt tcagatcgtc
      181 cgcccgcaga tggtcataag tgtccatgtt ggtatggtgc gtgcgcgtac tatattcgat
      241 ttcgtcctgg atgaattgga agccaggcag gccgaccgca tcgaacgact ggtggtctgt
      301 gccgccggtg ttgctgatgg tgacggttgt tgcaccgaga tcgcggaaag gttccagcca
      361 ttgcgtaaat atggggcggc acgcttcatt accctgcaga tatatgcctc ttacttttcc
      421 ggttccattg tcgatattga aatagccgga cagcttgctg tgttcgggca ggagtttcat
      481 gtcggcgggg tcagcgaagg tcttctttac gtagccacgg gatcccagca ggccttcctc
      541 ttcgccgctc cacagggcga tcctgatagt gcgccgcggt ttgataccaa gggttttcaa
      601 tatcctgacc acttccatca tgaccgtaca tccggcagcg ttgtccgttg caccggtgcc
      661 gctatgccag gaatcaaggt ggccgccgag cattacgatc tgatccttaa gctgcgggtc
      721 tgtgcccttg atctccgcga tgacgttata accctttgta tcgttcgtgt cgaacttcgt
      781 tttcacatcc acgtcaagac tcaccggtgt acccgcctta caaaggcgaa caatcgtcat
      841 ataatcttcc aatgcgatct caatgtcgag gaaattcggt ggcgcatcgg cgcgatatgc
      901 gccccctccc tgaacgaaga gtgtgccgtc gtgtccgcgc gcagactgac tcagaatggc
      961 aatggcaccc tcttcctcag ccattttctt gagtgcgttg acgaggcgct ggccaccgcc
     1021 acggaggaaa gcttcgcgcc gacgacgaat ggctgcggta tcggggggac taaaaacaac
     1081 atgtgccatc gaatccaact gtgcatcggt ccagcggctc gcgtctggct taaagctttg
     1141 ttcgtaaact tcatgggagt ccatgatcag gaccttacca gcgagtttcc ctttatattg
     1201 gtcgagatct gtggaatctt ttgcttcaat gagaacgacc tgtgcggttt gaggaccgtt
     1261 cgtaccggcg caccacgctt tagggaaggc aatcaggggc ttgtagtacg gcgctgaaag
     1321 ggcgacgtag gatttttcca gttcccagcc ttttccgaat tcaccccagg gatcgagcgt
     1381 agcattttcg aggccccact gcgccagttc ttccttggcc cagttggcgg cgcgaaagaa
     1441 tcctgccgaa ccggtgaggc gatcgccgct ggcatcggtc agatggaagg caatgtccat
     1501 cacgtgcgag tcctgcagcc cttcctgccg gatcttttgg atggtggcca tgtcgatctt
     1561 ttcgttctgg gccgtcaatc cggcggagac aagcagaaaa gcgacgaggc ttagcgatag
     1621 tcgggagcgt tttctcatga gcgttgcatt ttaggtcaat gtaaaagatt gcagggtaac
     1681 gggcaaataa aatcggaaaa gcggcagtta aatgtatgag cggtgaatta cctttgttta
     1741 tgcgaaaatt aaggatggac gagctggacc ggaaatcggt gaatgacttc aggcaggcgg
     1801 aaaagatacc gatcgtggtg gtcctggaca atatccgcag catgcacaat gtgggcagcg
     1861 tcttccgtac ggccgatgca tttcttattc gcgcgattta tctttgcggt tatactccgg
     1921 tgccgcctca tcgggacatt cacaaaacgg cccttggggc aaccgaaacc gtggcctggc
     1981 gtcattttgc tgccgttacc gatgcgatcg ccgggttgaa agacgaaggg tatgcagttg
     2041 ttgccgtgga gcaaacggag aaaagtgtcg cgctttcagc gatccgggac cgtttccccg
     2101 gcccgcttgc ggtcgttttc ggaaacgagg taggcggagt gaccgagcag gcgcttgcgt
     2161 tgtgcgacgg gagtgtcgaa atcccgcagt ggggaatgaa gcattctctg aatgtatcgg
     2221 ttgccgcggg catcgtattg tgggaatcgg tgcggcagcg cactctataa tataaatatg
     2281 agcactttca agagttattt gagcttgatc aggttttcgc acacgatctt tgcgatgccg
     2341 ttcgcgatga tcggtttttt tttgggcgtt gcagagctca cctattggga caggaccggg
     2401 gcgatagcct cgggcggtca gggatggcat gttacggatt ggcgaatgga ctatttcctt
     2461 ttcaaatttg tgctggtcat cctctgtatg gtctttgccc gcagtgcggc gatggcgttc
     2521 aaccggtggc ttgatgttaa attcgacgcg ctcaatccgc gcactgctat ccgggaaatc
     2581 cccgccggga tcatttctaa gaacagcgcg ttgcgattcg tggcggtcaa ttgcgtttta
     2641 ttcatccttt ccacctggtt cattaatccc ttgtgttttc tgttgtcgtt tgtcgcactc
     2701 gcggtggtgc tgggctacag ctatacgaag cggtttacgc cgctgtgcca tctcgtcctg
     2761 ggcctggggt tgtcccttgc gccgatcggg gcctatttgg cggtcgcggg caggttcgcg
     2821 tggctgccca tcctcttttc gctaacggta atcttttggg ttggcggatt cgatattatt
     2881 tacgccttgc aggacgaaga atttgacagg actcagcagc tttattcaat gccttcctgg
     2941 ctgggaaagg cgaaagcact tcgcgtctcc gaaattctcc atctgctgag tacagcgtgc
     3001 gtggtaacgg ccggtattta tggtggtttt ggctgctggt attgggctgg cgttgcggtc
     3061 tttatcggca tgttgattta ccagcactcc attgtaaagc cgggcgatct cagccgggtt
     3121 aacctcgcct ttatgaccgc gaatgggatt gcaagcgtcg tattcgcggg gttggtcatt
     3181 gctgacctct attggtatgc gcatatttaa ggttaatgcg cataaattga aaagatgcgt
     3241 atctttgcgc caacgtatga aagagcggtt gaacttaacc atcgacggag cattactggc
     3301 agcaatgaag gtctatgcgt ccagcaaagg gataagtatc tccgaattgg tcgaaagcta
     3361 tttccgcgcc gtcactaagc cattaaatcg aaagaatatc ctccacctcg tggaggaact
     3421 ggatccgccg gggatctctg ttgaccaggc tgccggcggc aagaagccgc ttgcagaacc
     3481 ggaggcggag ctactttcgc cggcgaggca gccggaacct ttgacaaaat ttaaaagggg
     3541 gaggcat
//
LOCUS       DATGKE010000601         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_34371, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000601 DATGKE010000000
VERSION     DATGKE010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34371"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VKU83_10215"
     CDS             <1..1739
                     /locus_tag="VKU83_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HLI93976.1"
                     /translation="GGMVTMAPGTAEPAIEFTVTEREPGIETAGLVAGAEPVEDWAKE
                     PQELGDPELEVTQGPDSIPAAEEGEAGDPFLREDLPDYEPTLDLRDYRYPALDLLEAH
                     GSERIVQDPSELEANKNQIITTLRNYDITISKISATVGPTVTLYEIVPAAGVRISRIK
                     NLEDDIALSLAALGIRIIAPIPGKGTIGIEVPNVKKTVVSMRTLLSSDKFQNSNFNLP
                     IAIGKKIDNENFIVDLTSMPHLLMAGATGQGKSVGLNAILVSLLYKKHPAQLKLVLVD
                     PKKVELSIYRTIEKHFLAKLPGEEESIITDTKKVIHTLNALCIEMDNRYDLLKEASAR
                     NIKEYNEKFVKRRLNPQKGHQYLPFIVLVVDEFADLIMTAGKEVEMPIARLAQLARAV
                     GIHLIIATQRPSVNIITGTIKANFPSRIAFKVSSKIDSRTILDAGGADQLIGKGDMLI
                     SHNGEITRLQCPFVDTPEVERITDFISRQRGYPEAYKLPEYVDEREMEGREFDLADRD
                     PLFEEAARLIVMNQLGSTSLIQRKMKLGYNRAGRLMDQLEAAGVVGPGMGSKPRDVLI
                     KSEMELDQYLRP"
     gene            1862..2503
                     /locus_tag="VKU83_10220"
     CDS             1862..2503
                     /locus_tag="VKU83_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HLI93977.1"
                     /translation="MGVCLAVEGQNTKYIGKNDPDAKKVLDQLSAKLKTYKAVQANFT
                     LKVEDAKGKPQGSRSGVIYLKGNKYHVSVAGGQEIYCDGKDVYTYDKSSNEVTITRND
                     PATQTISPDKLFTDFYDKDYLYKLNGNVKMGGRTVEEVELTPVDKSKNFFKALLYIDK
                     LSHSLVGIKWFDKGGNRYTLDTGRINGNASITDSQLAYNKAKFPGAEEVDLRN"
     gene            complement(2545..>2827)
                     /locus_tag="VKU83_10225"
     CDS             complement(2545..>2827)
                     /locus_tag="VKU83_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HLI93978.1"
                     /translation="ATGISSDLITRAADVILKERRKLICVIRDTPYNLIHIRNMEAVT
                     LAGGIICPATPSFYSRPASIEAAAATVVDRVLDLAGLDVITFRWGSEAD"
BASE COUNT          720 a          720 c          840 g          547 t
ORIGIN      
        1 aaggcggaat ggtaaccatg gcgcccggga ctgcggagcc ggccattgaa tttacggtga
       61 ccgagaggga acccgggatt gaaaccgccg gactggttgc cggcgccgaa cccgtggaag
      121 attgggcgaa ggaaccgcag gaattgggag atcctgaact ggaggtcacg caagggccgg
      181 atagcatccc cgccgcggaa gaaggcgaag ccggggaccc gttcctccgc gaggacctgc
      241 ccgattacga gccgacgctg gatctccggg actaccgcta tcccgcgctg gacctgctgg
      301 aggcgcacgg cagtgaaagg atcgtccagg atccctccga actggaagcg aataagaacc
      361 agatcatcac gacactccgc aactacgaca ttacgatttc caagatcagc gctaccgttg
      421 gaccgaccgt aaccttgtat gagatcgttc cggcggcagg agtgcggata tcacggatca
      481 aaaacctcga agacgatatt gccctgagcc ttgccgccct gggtatccgg atcatcgccc
      541 ccatccccgg caagggaacg atcggaattg aggtgccgaa tgtgaagaag acggtggtca
      601 gcatgcgcac cctgctatcc tcggataaat tccaaaacag caatttcaac ctgccgattg
      661 cgatcggaaa gaagatcgac aatgagaatt tcatcgttga tctgacctcc atgccgcatt
      721 tgctgatggc gggcgcaaca ggccagggaa agtcggtggg gctgaatgcg atcctcgttt
      781 ccctgcttta taaaaagcac ccggcgcagc tcaaactggt actggtagac ccgaagaagg
      841 tggaattaag catttacagg actatcgaga aacatttcct tgcaaaattg cccggcgagg
      901 aagaatccat catcacggac accaagaagg tcatccacac gctgaatgcc ctgtgcattg
      961 aaatggacaa ccgttacgac ctgctgaaag aagcgagcgc tcgaaatata aaggagtaca
     1021 atgagaagtt tgtgaagcgg cggctgaatc ctcaaaaggg gcaccagtat ctgcctttta
     1081 ttgtgctggt ggtggatgag tttgcagacc tcatcatgac ggcgggaaag gaggtcgaga
     1141 tgcccatcgc acgactggcg cagcttgcgc gggcggtagg gatccatttg atcatcgcca
     1201 cccagcggcc gtcggtgaat atcatcacgg gaacaatcaa agccaatttc ccttcacgga
     1261 ttgccttcaa agtgtcgagc aagatcgatt cccgcaccat ccttgacgca ggaggagcgg
     1321 accaactgat cggcaagggc gatatgctta tctcgcacaa cggggagatc acccggttgc
     1381 aatgcccgtt cgtcgacacg cccgaggtcg agcggatcac cgatttcatc agccggcagc
     1441 ggggctatcc ggaggcatat aaattgccgg agtacgtcga tgagcgcgag atggaaggca
     1501 gggaattcga cctcgctgac cgcgatccgc tattcgagga agccgcccgc ctgatcgtga
     1561 tgaatcagct ggggtcgact tcgctgatcc agcggaagat gaaattggga tacaatcgcg
     1621 ccggcagatt gatggatcag ttggaagcgg ccggcgttgt cggcccgggg atgggaagta
     1681 agccgcggga cgtattgatc aagtcggaga tggagctgga ccaatacctc aggccctgaa
     1741 ctcctgatcg caaacctcac cataacccgt aaaatttggc gtgcatgccg cgggcagccg
     1801 ctttttgcgt taatattgcg ccatgagaat gaccaaaata tcactttgtc taatgctttt
     1861 tatgggcgtt tgtttggctg tggagggaca gaataccaaa tacatcggca agaatgaccc
     1921 ggatgccaag aaggtactcg accaattgag cgccaaactg aaaacctaca aagcggttca
     1981 ggccaatttc accttgaagg tcgaggatgc aaagggaaaa ccacaaggga gccggagcgg
     2041 cgtgatctat ttgaaaggga acaagtacca cgtcagcgtg gcgggcgggc aggaaatcta
     2101 ttgcgacggc aaggatgtgt atacttatga caagtcttcc aatgaagtga ccataaccag
     2161 gaatgatccg gcgacgcaaa ccatttcccc cgacaagctg ttcacggatt tctatgacaa
     2221 ggactatttg tacaagctta acggcaacgt gaagatggga ggccgcacgg ttgaggaggt
     2281 ggaactgacg ccggtggata agtccaaaaa ctttttcaaa gccttgttgt atatcgacaa
     2341 gctaagtcat agtctggtgg gtatcaagtg gttcgataag ggaggaaacc ggtatacgct
     2401 ggatacgggc aggatcaacg gcaatgccag cattacggat agccagctgg cctacaataa
     2461 ggcgaagttt ccgggcgccg aagaggtgga cctgcggaac tagggggcgc agcacagtca
     2521 cggctccgct catcggttgg catgtcaatc cgcctcggag ccccagcgaa aagtaatgac
     2581 gtcgaggccg gccaggtcga ggacgcgatc cactaccgtg gctgccgccg cttcaatgct
     2641 ggctggccgg ctatagaagg aaggggtggc ggggcagatg atcccgccgg ccagggttac
     2701 ggcctccatg ttgcggatat ggatcaggtt gtaaggcgta tcccggataa cgcaaattaa
     2761 tttccttctc tctttcagga tgacgtccgc cgcccgggtg atgaggtctg atgaaatgcc
     2821 ggttgcg
//
LOCUS       DATGKE010000602         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_139018, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000602 DATGKE010000000
VERSION     DATGKE010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139018"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VKU83_10230"
     CDS             <1..315
                     /locus_tag="VKU83_10230"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HLI93979.1"
                     /translation="KLMDAATVLGACGTAFAMRYIRANIQGGIEIGFDAATANLIAAQ
                     TVKGAAELLLRRGTHPEQEIDKVTTPKGCTIAGLNEMEHKGFSSSLIKGLVTSYEKIA
                     RD"
     gene            complement(339..1436)
                     /locus_tag="VKU83_10235"
     CDS             complement(339..1436)
                     /locus_tag="VKU83_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93980.1"
                     /translation="MTLPTPPPGRAACCLSIASLCCLALTSGGQSERSGQDGSLSGLR
                     PLIIQLDNARQPLSATHNSPFGYFEVIDARADTAIIGVQGNLPMRSHAFDRQLVFRDP
                     AAREIGTYLNRLFIHPGSPDTAVVVLRCLWLSDTDPLTDNHDAVTNYENAPRRTHIRV
                     RAEIYARHNGRYLPLIRVDTLQPTRLVSYSILRSTYLGWEKELALLFNHVMEKAVLAY
                     ARKAASGRRITWEDIRRFNTARFDIPIFDTTALKRGVYTAFEEFRNNDPSIHDFEVLP
                     ENGDLALYLKNGDGTSYYTHNAWGFCNGKQVFLMRDGLLHLLHKDGNLFYFYGIDIPS
                     ADLLALPLSVFSGSQEQHCIYVVDMDTGQFY"
     gene            complement(1484..2527)
                     /locus_tag="VKU83_10240"
     CDS             complement(1484..2527)
                     /locus_tag="VKU83_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93981.1"
                     /translation="MRYRTLHTRTFPAFFVLLALHSTAQSSAPLSGLRAVEVRLNQRS
                     PAPGPPASYSFRHFDVVDLRADTSRIGIHTRIPDFGPNFDRQLVFARPAAVQIAAYLN
                     HRFTNPAASLSALVILRTLWLSDANVIRPELLRHPERRLERAHIRIKVEIYAIRDGQY
                     LPVLRFDTLQTTWKKRILSERSPYLEWGRNLSLLIDEFADSASRLTVERERGAQWVDL
                     EAIRRSDSLRFDAPIDERSPARGVYASFEEFRNNSPSITDFEIRVTNRERLLYVRQSG
                     TAYYCHDAWGYSDGKDIYIMRDGVLCPAWKEEKAFYIPAAARDTSEAPLNRSRGYRGR
                     TIYFVDMDTGDIY"
     gene            2781..2966
                     /locus_tag="VKU83_10245"
     CDS             2781..2966
                     /locus_tag="VKU83_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93982.1"
                     /translation="MKKGKTLTALVLVLEIATITVLHAVKISQSEKAGTKEISRNTAQ
                     EPVDAKPHTTYTLAAIR"
     gene            3259..>3824
                     /locus_tag="VKU83_10250"
     CDS             3259..>3824
                     /locus_tag="VKU83_10250"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLI93983.1"
                     /translation="MAKYVFVTGGVTSSLGKGIIAASLAKLLQARGLRVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIFTSQANNVTTGRIYQTVINKE
                     REGAYLGKTVQVIPHITDEIKRRMLLLGQGNQYDIIITEIGGTVGDIESLPFIEAVRQ
                     LQWEMSEEDCVVLHLTLIPYLKAAKELKT"
BASE COUNT          931 a          920 c         1118 g          855 t
ORIGIN      
        1 aagctaatgg atgcggctac ggtgctgggc gcatgcggga cggcgtttgc gatgcggtat
       61 atccgggcga atatccaggg cggcatcgag atcgggttcg atgccgcgac ggccaacctg
      121 attgcggcgc agacggtgaa gggagcggcg gagctgttgt tgaggcgggg cacgcatccg
      181 gagcaggaga tcgacaaggt gacgacgccg aagggctgta cgatcgcggg cttgaatgaa
      241 atggagcaca agggtttcag ttcgtcgctg atcaaggggc tggtcacgag ttatgaaaag
      301 atcgcgaggg attaggcttc aatagaaagg attaggcttc aatagaattg acccgtgtcc
      361 atgtccacga catagatgca atgttgttcc tgcgagccgc tgaataccga aagggggagt
      421 gcgagcaggt cggcggaggg aatgtctatc ccgtagaaat aaaacaggtt accgtcctta
      481 tgaagcagat gcagtaaccc gtcgcgcatg agaaatactt gtttgccatt gcagaatccc
      541 caggcattat gagtgtaata agacgtcccg tcgccgttct tcaggtacag cgcgaggtcg
      601 ccgttttccg gtaatacttc gaaatcgtgt atcgacgggt cgttgttccg gaactcttcg
      661 aaggccgtgt aaacgccccg cttcaacgcc gtcgtgtcaa agatgggaat atcgaagcgg
      721 gctgtattga aacgccggat atcctcccag gttatccttc ttcccgaggc tgcttttcgg
      781 gcataggcaa gaacggcttt ttccattacg tggttaaaca gcaaagccag ttccttttcc
      841 caaccgaggt aggtcgacct caggatgctg tatgaaacca gccgggtggg ttggagggtg
      901 tcgacgcgga tcagcggcag gtaccggccg ttatgcctcg catatatttc agcgcggacg
      961 cggatatgcg ttcggcgcgg tgcattttca taattggtga cggcgtcgtg gttatcggtt
     1021 aaggggtccg tatcggatag ccagaggcac cggaggacca ccacagccgt gtccgggctg
     1081 ccgggatgga taaagagccg gttcagataa gtgccgatct cccgggctgc cggatcgcgg
     1141 aaaaccaact ggcggtcaaa ggcgtgactg cgcatgggca ggttgccctg aacgccgatg
     1201 atcgcggtgt cggcccttgc gtcaatcacc tcgaaataac cgaaaggact attatgggta
     1261 gccgataaag gttgacgggc attgtcaagc tgaatgatca gcggccggag gccggaaagg
     1321 cttccgtcct ggccggatct ttcggactgg ccgccgctgg tgagggcaag gcaacacagg
     1381 cttgcgatcg acaagcagca cgcggcgcgg ccagggggag gtgtcgggag tgtcatttgc
     1441 aacggttcat tgggggtgaa gagttattcg tgggcgctcc cggtcaataa atgtctccgg
     1501 tgtccatatc gacgaaataa atagttcttc cgcgataacc acgacttcga ttcaaaggag
     1561 cttccgaagt atcccgggcc gcggctggga tatagaaagc tttttcctcc ttccaggccg
     1621 ggcacaagac cccgtcccgc ataatataaa tatccttgcc gtcgctgtat ccccacgcgt
     1681 catgacagta ataggcggtt cctgactggc ggacgtagag gagacgctcc cgattggtca
     1741 cccggatctc aaaatctgtg atcgagggac tgttatttcg aaattcctca aagctggcgt
     1801 aaacaccgcg cgccggagac ctttcgtcga ttggcgcatc gaagcggagg gaatctgacc
     1861 gccggatcgc ttccaggtcg acccattggg cgccgcgttc tctttcaacc gtcagccggg
     1921 aggcgctgtc cgcaaattca tcgatgagaa gggacaggtt acgcccccac tcgagataag
     1981 gcgatctttc ggaaagaatg cgtttcttcc aggtcgtttg cagggtatca aacctcagta
     2041 cgggaaggta ttggccgtca cgaatcgcat agatctcgac ctttatccgg atgtgcgcgc
     2101 gttcgagccg ccgttcggga tggcgcaaca gttccggccg gatgacgttg gcgtccgaca
     2161 gccaaagagt gcgcaggata acgagtgcgg atagagatgc agccggattg gtaaaccggt
     2221 ggttcagata ggccgcgatt tgcactgcgg cgggccgggc aaaaaccagc tggcgatcga
     2281 aattcggacc gaagtccgga atgcgggtat ggattccgat ccggctcgtg tcagcgcgaa
     2341 ggtcgactac gtcgaaatgc ctgaaggaat aggatgccgg cggaccaggc gccggggaac
     2401 gctgatttaa cctgacctcg acagcccgga ggcccgacag aggtgcggag gattgggcgg
     2461 tggaatggag cgccaaaagc acaaaaaaag caggaaaagt acgggtgtgc aaagtacggt
     2521 agcgcataag ctggtgatgc caaacaagat actaaaaaaa cttctttttc ttaaatgatt
     2581 tatttgttat atatatagct gattgttaaa ggcttaggac cgaaggtttg agccaaaaga
     2641 tcgtaaaaac cctatgggcg gtcaggaatt tccactattg ccagcaaaat aatccgatac
     2701 tatattgcgt tctattgttt cttatccgcg tgcccggaaa ggcacaaacc ttatcgaagc
     2761 aatatttaat ccaatttacc atgaagaaag gaaagaccct gacagccctt gtactcgtac
     2821 tggaaatcgc taccatcacc gttttgcacg ccgtgaagat cagtcaatcg gaaaaggccg
     2881 gcaccaagga gatttccagg aacactgcgc aggagcctgt tgatgcgaaa ccccatacca
     2941 cctatacgct tgcggcgatc cgttaagaaa tgttgaccga aaatgaatga gcaaataagg
     3001 cccgcccgcg ggccttacgt gtttttggct agttatcagc aatttgccgg ctagttatcc
     3061 acatcaataa atccgtattt ttccgcaact gaaattcacg tacttttgta cgacctcttg
     3121 gaatttcttt tttctatttt ttttcagcag gtcaagcagt tgctgggctc gcgcgccggt
     3181 agtttgatgg tattatatca tcggccggtg aaccattgga gcgagaatgg attgctaaga
     3241 taattaattg accattacat ggctaagtat gtttttgtta ccggtggtgt aacgtcgtct
     3301 ttggggaagg ggatcattgc agcctcgctt gccaaattat tgcaggcaag aggcctgagg
     3361 gttacgattc aaaaatttga tccctatatc aacgtagacc ccgggacgct gaatccatac
     3421 gagcatgggg aatgcttcgt cactgaggac ggggccgaga cggatctgga ccttggccat
     3481 tatgagcggt tcctgaacat tttcacgtcc caggcaaaca atgtaaccac cggcaggatc
     3541 tatcagacgg tgatcaacaa ggaaagggaa ggcgcctatc tcggcaaaac cgtgcaggtc
     3601 atcccgcata ttacggacga gatcaagcga aggatgttgt tgcttgggca gggcaatcaa
     3661 tacgatatta tcattaccga gatcggtgga accgtgggcg acattgaatc cctgcctttc
     3721 attgaggctg tccgccagtt acagtgggag atgtcggagg aggactgcgt cgtgctacat
     3781 ctgacgctca tcccttactt gaaagcagcc aaggagttga aaac
//
LOCUS       DATGKE010000603         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_139505, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000603 DATGKE010000000
VERSION     DATGKE010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139505"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VKU83_10255"
     CDS             <1..473
                     /locus_tag="VKU83_10255"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLI93984.1"
                     /translation="GLLRVGGTDTYISEIVFIDRLDEADYRGGQLNGPVTPVVIQCIE
                     QLIQYFHGQRRVFDFPICQEGTEFQQRVWNELMGIPFGKTISYLELSRRLGDTKNIRA
                     AAAANGKNNIVIVVPCHRVIGSRRDLVGYGGGLWRKKWLLDHETRVQWGVQTLF"
     gene            470..1531
                     /locus_tag="VKU83_10260"
     CDS             470..1531
                     /locus_tag="VKU83_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HLI93985.1"
                     /translation="MIQRVSDIVPEAADMKSLFLEPADGEPLRYEAGQFLTLLFSFQG
                     RELRRSYSFASTPGIDPVPAVCVRRVPNGEISRHLLDHLRVGDLLTTLEPAGRFTLGA
                     LEAGNVIFVGAGSGIVPIYSLLKEALALRPHLRVQLLTQQHDVAASPFRAALQGLEAS
                     FGAHRLEWTEMLTVRDGRMHRGWLEEWVLARGRATLDGQYYVCGPPDFMRMVQFTLRT
                     LGVAAERIRREHFTVGRIAPAPPVFDATPKRITIRDTEITREFTAVWPESILDAALRQ
                     GIALPYSCRAGRCSSCVARCLAGRVRMTNNEVLTENDLREGLVLTCTGYAETDVELSY
                     ERSSAPNPTGNNAPERGMT"
     gene            complement(1468..1923)
                     /locus_tag="VKU83_10265"
     CDS             complement(1468..1923)
                     /locus_tag="VKU83_10265"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HLI93986.1"
                     /translation="MEYPSDGELRPEDSFLLKKARSATRDAYAPYSHFRVGAAAVLAN
                     GEIITGVNQENAAFPAGICAERTLLSVAAARFPGIPIETLAISYYNEQGPADRPISPC
                     GICRQSLKEFEDRTHHPIRLVLGGQTGPVYVIPRSGALLPVGFGAEDLS"
BASE COUNT          392 a          603 c          635 g          415 t
ORIGIN      
        1 tgggcctgtt gcgggtgggg ggtacggata cctatatcag tgagattgtc tttatcgatc
       61 gcctcgacga ggccgactat cgtggcggac aactcaacgg cccggtcacg ccggtggtga
      121 tccagtgcat cgaacaactc attcagtatt ttcacgggca gcgcagggtc ttcgactttc
      181 ccatctgcca ggaaggcacg gaattccagc aacgcgtctg gaatgaactg atggggatcc
      241 cgtttggaaa gacgatcagc tatctcgagc tgtcccgccg cctgggcgac acgaagaata
      301 tccgcgccgc cgccgctgcc aacggtaaaa ataatatcgt catcgtcgta ccctgtcacc
      361 gggtgatcgg ctcgaggcgc gatcttgtcg gctacggcgg cggactgtgg cggaaaaagt
      421 ggctccttga ccacgagacc cgggtacagt ggggcgttca aaccttgttt tgatccagcg
      481 cgtttccgat atcgttcccg aggcggccga tatgaagtcc ctgttcctgg agccggccga
      541 tggcgagcct ctccggtatg aagccgggca attcctcacc ctgctctttt cctttcaggg
      601 tcgcgagctt cgccgcagct attccttcgc gagcacaccg ggcatcgatc cggttccggc
      661 cgtctgcgtc aggcgcgtgc ccaacggcga gatctccagg catctgctgg accacttgcg
      721 ggtgggcgac ctgctcacca cccttgaacc ggccgggcgg ttcacactcg gcgccctgga
      781 agccggcaac gtgatcttcg tgggcgcggg cagcggcatc gtcccaatct attcgttgct
      841 gaaggaggca ttggcgcttc ggccgcacct ccgcgttcaa ttgctgactc agcagcatga
      901 cgtggcggct tcgccgtttc gggcggcact gcaaggactg gaggcgtcgt ttggcgctca
      961 ccggctggaa tggacggaaa tgctgaccgt gagggacggg cggatgcatc gcgggtggct
     1021 ggaggaatgg gtactggcaa ggggccgggc gacgctggac gggcaatatt atgtatgcgg
     1081 accgcccgac ttcatgcgaa tggtgcagtt taccctgcga accctgggcg tcgcggccga
     1141 acggatcagg agggagcatt ttacggtagg acggattgct cccgcaccgc cggtatttga
     1201 tgccacgccg aaacgaataa cgatccgcga cacggaaatc acccgggaat ttaccgccgt
     1261 ctggcccgaa agcatcctgg atgcggcact tcgacagggc atcgcccttc cctacagctg
     1321 ccgggcgggc cgttgctcga gctgtgttgc ccgttgcctt gccggccgcg tccggatgac
     1381 gaataatgaa gtgctgaccg aaaacgacct gcgggaaggc ctggtgctga cttgcaccgg
     1441 ctatgccgaa acggacgtcg agctgagtta cgaaaggtct tccgccccga atccgaccgg
     1501 caataatgca cccgagcgcg gaatgacata aaccggcccg gtctggccgc cgagaacaag
     1561 ccggatggga tgatgggtgc ggtcctcgaa ttctttcagg ctttgccggc agataccgca
     1621 gggcgaaata ggccggtcag ccggcccctg ttcgttataa tagctgatgg ccagggtctc
     1681 aatggggatg cccgggaacc gcgcggcagc gacggatagc agcgtccgtt ccgcacagat
     1741 accggccggg aaagcggcat tctcctggtt gacgccggtg atgatctcgc cgtttgcaag
     1801 caccgcggca gcgcccaccc ggaaatgaga atagggcgca taggcgtccc ttgttgcgct
     1861 cctggctttt ttgagcagaa aggaatcctc cggccgaagt tccccgtccg atggatactc
     1921 catataagtc aatttgactt cccgctcctg catacgatcc gtttaaagga gaatagatgc
     1981 ctttttcatg gtgactacct gatcttcgtg aacagcctgt tccagcgaac gcccttgttc
     2041 cagct
//
LOCUS       DATGKE010000604         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1280490, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000604 DATGKE010000000
VERSION     DATGKE010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280490"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3404)
                     /locus_tag="VKU83_10270"
     CDS             complement(<1..3404)
                     /locus_tag="VKU83_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLI93987.1"
                     /translation="MTWKKILLVFVLASALSRLFAQSTEYQFSTLNFSNGLSNNHITC
                     LYKDPHGFMWFGTMSGLDRYDGYEFKIFRHDQHDPYSIGDNYIEQIFEGPEGKMWVQS
                     RSSRFNIYDLATDRFDQDYAGYLRKHSLPEFWLLAISHSATGFWFVYRDSGIFHLAAD
                     GRVFGVRQNDQVGGCLSAVSITDAKEDHDGNCWVVHQNGLLEKVDARSHRVVFRTLEL
                     EKVFGHNQIPCGIYIDNQNDLWLYSAGYFKGVWCYHPPTGTFTHFSASSVEAFAKADH
                     SSANIGRRSPGEGGALVKALPSPADNLAKAGRLSSDVIYTALQDEKGKIWLATDHGGV
                     DILDKASYSVTALTHIEDDRKSLAENSIPAMIMDSTGTVWLGTFKSGINYYHQKGIQF
                     PLYRHHPGDAHSLSFDDVNKFVEDSAGNIWIGSNGGGLIYFDRPHNRFRQYLNDPRNP
                     NSLSNNIIVSLLVDHNNRLWIGTYFGGLDCYDGKRFIHYRHDDHRPGSLADDRVMCLY
                     EDCDHNLWVGTLAGGLDRFDRKTDSFEHFNSSRPNSILNNYVSSIVEDREHNLWVATA
                     YGIDVLDKRNGRFIHYSSERNQLSTDNVILLFRDSRYNMWVATRDGLNLFVPGKNSFQ
                     SFSTDNGLPDNTIRGIVEDQQHDLWVSTANGLSRISISAAGDRPGSPVRIHCRNYHEK
                     DGLQGREFNERTALATSDGCLLFGGANGFNLFRPADITNTADVPPIVLTGLDIFNKSV
                     QVGEKMGNHVILDKSLSETGEITLNHSEDVFSIEFAALDFIENAGDKYAYKLEGFNKN
                     WLVTDGGSRKATYTNLDAGEYTFRVKASDEDGQWYDREATLRIIVLPPFWKTPIAYAV
                     YALLLAAILLLARRMVIRKARMRFALEQERRETRRLHEIDLMKIRFFTNMSHELRTPL
                     SLILAPVEKLLAAGPAAGSDPRRTDSSAFSTRLPSASDPRPQYELIRRNARRLLHLVN
                     QLLDFRKMEVNELRLHPRQGDILKFIREISFSFIDLAERKNIAFTYTSDNESLITSFD
                     HDKMERILFNLLSNAFKFTPAHGAVSVIVYTECTEDLATITIKVKDSGIGIPPDKLEK
                     IFDRFFQSEVPDTILNQGSGIGLAITQEFVRMHQGQLTVESQIGQGS"
     gene            3672..>4230
                     /locus_tag="VKU83_10275"
     CDS             3672..>4230
                     /locus_tag="VKU83_10275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI93988.1"
                     /translation="MNLTAFVGVFLASCLTLSLSAQPPGARRITGKVTDQANSQGIPG
                     ATVTVKGTRNSVQTDVNGSFEILAAPTESLVVSYVGYSSKEVKVGNATTLNITLSTNY
                     GHMDDVVVVGYGRMKKSDLSAAVGTISNEDLNKTVNVTLDDALQGKSPNVYVSQTSGE
                     PGAGASVIIRGVSTITGNYQPLYVID"
BASE COUNT          940 a         1060 c         1239 g          991 t
ORIGIN      
        1 gatccctgac caatctggct ttcgactgtg agctggcctt ggtgcatgcg cacaaattcc
       61 tgcgtgatgg cgaggccgat tccgctgccc tggttgagga tggtatcggg cacctccgac
      121 tggaaaaagc gatcgaagat cttctccagc ttatcgggcg gaatgccgat tcccgagtct
      181 tttactttta tcgtgatcgt cgccagatcc tccgtgcatt cagtgtaaac gattacactg
      241 acggcgccgt gtgcgggcgt gaatttaaag gcgttggaca ggaggttaaa caggatgcgt
      301 tccatcttgt cgtggtcaaa ggaggtaatc agcgactcat tatcagaggt ataggtaaaa
      361 gcgatgttct tcctttcggc caggtcgata aaagaaaagc tgatctcccg gatgaatttg
      421 aggatgtcgc cctggcgcgg gtgaaggcga agctcgttga cctccatttt gcggaaatcg
      481 agcaattgat tgacaagatg cagcaggcga cgggcgttgc ggcggatcag ctcgtactgc
      541 ggtcgcgggt cggacgcgga tggcagccgg gtgctgaagg cggacgagtc ggttctacgc
      601 gggtcggagc ctgcggccgg gccggcggca agcagttttt caaccggtgc aaggattagg
      661 gaaagcggcg tccggagctc gtggctcatg ttggtgaaga aacgaatctt catcaggtcg
      721 atctcgtgca gccttcgggt ttcgcggcgc tcctgctcga gtgcgaagcg cattctggcc
      781 ttgcggataa ccatccgccg ggcgagcagc aggatagcgg cgagcagcag ggcgtaaacg
      841 gcataagcaa tcggcgtttt ccaaaaaggc ggaaggacga tgatccggag ggtagcctcg
      901 cggtcgtacc attggccatc ctcatccgat gccttgaccc tgaaagtata ttcgcccgcg
      961 tcgaggttgg tgtaggtcgc tttgcggctg ccgccgtcgg ttacgagcca gtttttgtta
     1021 aacccttcca gtttgtacgc atatttgtcg ccggcgtttt cgataaagtc gagtgcggcg
     1081 aactcgatgg agaagacgtc ttcgctgtgg ttgagggtga tttcaccggt ctcggagagc
     1141 gatttgtcga ggataacatg gttacccatt ttttcaccta cctggacgct cttattgaaa
     1201 atatcgagcc cggtcagcac gatcgggggg acatcggcgg tattggtaat atccgcgggc
     1261 ctgaaaagat tgaagccgtt cgcaccgccg aacagaaggc aaccgtcgct ggtggccagg
     1321 gcggtccgct cattgaattc ccggccctgc agaccgtctt tttcatgata gttgcgacag
     1381 tggatgcgga cagggctgcc gggacggtcg cccgccgcgc tgatgctgat ccgggaaagg
     1441 ccgttggcgg tgcttaccca caggtcatgc tgctggtcct ccacgatgcc ccggatggta
     1501 ttgtcgggga ggccattgtc cgtgctgaag gactggaagc tgttctttcc cggaacgaag
     1561 aggttcaggc cgtcacgggt cgcgacccac atgttgtacc ggctatcgcg gaacaggagg
     1621 atgacattat cggtgctgag ctggttccgc tccgacgagt agtgaataaa gcggccgttg
     1681 cgcttatcca gcacgtctat gccgtaggcg gtagcgaccc ataggttgtg ttcgcggtct
     1741 tcgacgatcg aagaaacata attattaagt attgaattcg gcctgctgct attgaaatgc
     1801 tcgaaactat ccgtcttgcg gtcgaaccgg tcaaggccgc cggcaagcgt acccacccac
     1861 aggttgtggt cgcagtcttc atacaggcac atgacccggt cgtcggcaag gctgccgggc
     1921 cggtgatcat cgtgccggta atggataaac cgtttgccgt catagcaatc aagcccgcca
     1981 aaatacgtcc cgatccagag ccggttatta tggtcgacca gtaggcttac gatgatatta
     2041 tttgacaggc tattgggatt tcgggggtcg ttcaggtact gtcgaaagcg gttatgaggc
     2101 cggtcgaagt agatcaggcc gccgccgttg gatccaatcc agatattgcc ggcggagtct
     2161 tcgacgaatt tattgacgtc atcgaagctg aggctatggg cgtcgcccgg atggtgacgg
     2221 tagagcggaa actggatgcc cttctgatga taatagttga tcccgctttt aaaggtgccg
     2281 agccagactg taccggtgct atccataatc atcgccggga tgctgttttc ggccaggctc
     2341 ttgcggtcgt cttcgatatg ggttagggca gttacggaat aactcgcctt atcaaggatg
     2401 tccaccccac cgtggtccgt cgccagccag atctttcctt tttcatcctg caaggcggtg
     2461 tatatgacgt cgctgctgag gcgccccgcc ttcgctaaat tatccgcggg cgaggggagc
     2521 gcctttacta aagctccgcc ttcgccgggg ctccggcgtc cgatgttggc ggaggaatgg
     2581 tccgccttcg caaaagcttc gacggaagaa gcggaaaaat gggtgaaggt cccggtgggt
     2641 ggatggtagc accagacgcc cttaaaatag cccgccgagt aaagccacaa gtcgttctgg
     2701 ttatcaatat atattccgca gggaatctgg ttgtggccga agaccttttc taattccagg
     2761 gtccggaaaa cgacccggtg gctccgggca tctacctttt cgaggaggcc gttctggtgg
     2821 acaacccagc aattgccgtc gtggtcttcc ttggcgtccg tgatggagac ggcggagagg
     2881 cagcccccga cctggtcgtt ctggcgaacg ccgaagaccc ttccgtccgc cgccagatgg
     2941 aaaatgccgc tatcgcggta tacaaaccaa aagccggtgg cgctgtggct gatggcgagg
     3001 agccagaatt caggcaggga atgcttgcga agatagcccg cataatcctg gtcaaaccgg
     3061 tcggtcgcca ggtcataaat attgaaccgg ctggaacggg attgtaccca cattttgcct
     3121 tccggacctt caaagatctg ctctatgtaa ttatcaccga tggaatatgg gtcatgctgg
     3181 tcatgacgga aaatcttgaa ttcgtagcca tcgtaccggt ccagcccgga catggtgccg
     3241 aaccacatga acccgtgggg atccttgtaa agacaggtga tatggttatt ggacagccca
     3301 ttgctgaaat taagggtaga aaattgatat tcagtcgact gggcaaagag tcgggaaaga
     3361 gcggaggcaa gcacaaagac caacaaaatc tttttccagg tcataccgct atggggcgag
     3421 gttacagaaa gcagaaaatt agcagccgtc catacaaata tattaaatgt gtaggcggaa
     3481 atcgaacatt taaataccct ttttgacgaa tttgacgccc tccggggctg gggaatctcc
     3541 ctatttttaa gctgcgattc tttcattacc agaaaaaaat aaaccgattg cattgttttc
     3601 aaattattcc ctatttttat taaaccgatt gcatttttta tcgtttcata accaactcta
     3661 aaaagatcca tatgaatctg actgcttttg tgggagtctt tttggcttct tgtcttacgc
     3721 tttctttatc agcccaacct ccaggtgcgc gaaggattac cggaaaggtg accgatcagg
     3781 cgaacagcca gggaattccc ggtgcgacgg tgaccgtgaa aggaacccgg aattccgtcc
     3841 agaccgacgt gaacgggtcg ttcgagatcc ttgccgcacc gacggagtcg ctggtcgttt
     3901 cctatgtcgg gtattcgtcg aaggaagtaa aggtgggtaa cgctaccacc ctcaacatta
     3961 ccctttccac caattatggc cacatggacg atgtcgtcgt cgtgggttac ggccggatga
     4021 aaaagtccga tctgagtgcg gcggtcggga cgatttccaa tgaggatttg aacaagacgg
     4081 tgaatgtcac cctcgatgac gcattacagg gcaaatcgcc aaacgtctat gtgtcgcaga
     4141 cgagcggcga gccgggtgca ggagcctctg tcatcatccg cggggtcagc acgatcaccg
     4201 gcaattatca gcctttgtat gtgatcgatg
//
LOCUS       DATGKE010000605         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1253329, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000605 DATGKE010000000
VERSION     DATGKE010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253329"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VKU83_10280"
     CDS             <1..391
                     /locus_tag="VKU83_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93989.1"
                     /translation="VIPALREREKRGPQIPRTAENRHDDGHGFFYVDFSSLTVVRDTD
                     LGKNYTAAAILQRTGVGHELRRLLERGKLQIRRPADKIALHSGHPDTAETRQALLRQS
                     PQHDDIVDRHLAREQELHLSQRRGLRR"
     gene            complement(388..1161)
                     /locus_tag="VKU83_10285"
     CDS             complement(388..1161)
                     /locus_tag="VKU83_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI93990.1"
                     /translation="MQIKIYSSVQPPIVRVICDEAHRLGLTVTGHIPQGMDLIAGVDS
                     GMDMVNHIQYVPPILKQKPDRTIDWNDPKTQAGLDFIKEHGTVIDPTLGVFEMIFRSV
                     KDTISNMEPAFASLPEPLQVLFKTMGMPPPMAQKYKPVFHDMEKIVKALYDRGVPIVA
                     GTDMGFPGFSVDRELELYVEAGLTPLEAIRTATLVPALVMKQASSSGSLMPGRTADLI
                     IVSGDPLHSIRAIRNVQTVVKDGQVYDPVTLHRLAGFLH"
     gene            complement(1180..2463)
                     /locus_tag="VKU83_10290"
     CDS             complement(1180..2463)
                     /locus_tag="VKU83_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93991.1"
                     /translation="MKKYCLGLAVAGVLAGVGPAAGRAQGVSAESAKAAIAAGVAGTA
                     GVGEKGDSATFYLHKFAQNIGKETFYRTMSDSGVSYAVHFRFVDRGQPVPLEARLVVT
                     PDYEPISLWLKGKTCRFCTISDSVVIAGSTAAIRVDDSSFSRTVKGPTFPVGGYSPGT
                     VQMALLQYWRKHGRPTHLSLLPTGSVTIRQAGNDTLSQYGQRLLLERFVISGLIWGNE
                     LVWTDPQGNLICLITNDAEGDKLEMMLDRYEFFLPYLIGRAASYGMELFADEMKGEDK
                     HANAGALGQPGTLAIVGGNMIDVATGQTTTNATILIHNGRITKAGPSAGIPVPAGAKV
                     IHAEGKIILPGLWDMHAHFEQAEWGPAYLAAGVTTVRDCGNEFNYINSVKTAIDDGTG
                     IGPHILKAGIIDGPGPMGLGVIRASTQQEAIAAVR"
BASE COUNT          520 a          782 c          728 g          523 t
ORIGIN      
        1 ggtcatccct gctctccggg agcgcgaaaa acgcggcccg cagatccccc ggacagccga
       61 aaacagacac gatgacggcc atggcttttt ttacgtcgat ttttcgtcac tcaccgtcgt
      121 gcgcgacacc gatctcggta agaactacac cgccgccgct attcttcagc gtaccggggt
      181 cgggcatgag cttcgccggc tgctcgagcg gggcaaattg caaattcgca gacctgcgga
      241 caagatcgcc ctccattccg gtcatcctga tacggctgag acacgccagg cgctattaag
      301 acaatctccg cagcatgacg atatcgttga tcggcatctg gccagggagc aggagctgca
      361 cctgagccaa aggcgaggcc tgcggcgtta atgtaaaaat cctgccagcc ggtgcaaggt
      421 cacggggtcg tatacctgcc cgtccttgac caccgtctgt acgttgcgga tagcgcggat
      481 ggagtggagc ggatcgccgc tgacgatgat cagatccgcc gtccggcccg gcatcagcga
      541 accgctggaa ctggcctgtt tcatcaccag tgcggggacc agcgttgccg ttcggatcgc
      601 ttcgagcggc gtcaggcctg cctccacata gagctccagc tcacgatcca cgctaaagcc
      661 gggaaaaccc atatcggttc cggccacgat gggaacgccg cggtcataga gggctttcac
      721 gatcttttcc atatcgtgga atacgggctt gtatttctgc gccatcggcg ggggcatgcc
      781 catggtcttg aacaacacct gcagcggctc gggtagggaa gcaaaggccg gttccatatt
      841 gctgatggta tccttgaccg agcgaaagat catttcgaaa acgcctaggg tagggtcgat
      901 caccgttccg tgttccttga tgaagtcgag cccggcttgt gtcttggggt cgttccagtc
      961 gatcgttctg tcgggtttct gcttgaggat cggcggaacg tattggatgt ggttcaccat
     1021 atccataccc gagtccacgc cggcaatgag gtccatgccc tggggtatat ggccggtaac
     1081 ggtcaacccc aaacggtgcg cttcgtcgca gatgacccgt acgataggcg gttggacgct
     1141 gctatagatc ttgatctgca cgaaaccgga gtctttgtat tagcgcacgg cagcgatagc
     1201 ctcttgttgc gtggaggccc ggatgacgcc gaggcccatc gggcccggac cgtcgatgat
     1261 gcctgccttc aggatatgcg gaccgatacc cgtgccgtca tcgatagcgg tcttcaccga
     1321 gttgatgtaa ttgaattcat taccacagtc gcggaccgtc gtcacgccgg cggcgagata
     1381 ggcaggtccc cattcggcct gctcgaaatg cgcgtgcatg tcccagagcc cggggaggat
     1441 gatcttgcct tcggcgtgga tgaccttggc gccggccgga acaggaatgc ccgccgacgg
     1501 gccggccttg gtgatgcggc cgttatggat gaggattgtg gcgttggtgg tagtttgtcc
     1561 ggtagcaaca tcgatcatat tgccgcctac gatggcaaga gttccgggct ggcctagggc
     1621 gccggcattg gcgtgtttat cctcaccctt catctcatcg gcgaagagtt ccatgccgta
     1681 ggacgcggcc cggccgatca ggtaggggag gaagaattca tagcggtcca gcatcatttc
     1741 gagcttgtcg ccttcggcgt cgttggtgat caaacagatc aggttgccct gtgggtcggt
     1801 ccacaccagc tcgttacccc agataagccc gctaatgaca aatcgctcga gcagcagacg
     1861 ctgcccgtat tgggaaaggg tatcattccc cgcctgccgg atagtcaccg agccggtcgg
     1921 caacaacgaa aggtgagtag gccgaccgtg tttccgccaa tattgcagaa gcgccatttg
     1981 aaccgtgccc ggcgaatagc ctccaacggg aaacgtcggc cctttgaccg tgcggctaaa
     2041 actggagtcg tcaacccgga tcgccgccgt cgaccccgcg atgaccacgc tgtcactgat
     2101 cgtacaaaag cggcacgtct tcccctttag ccaaaggctt atcggctcat aatcgggtgt
     2161 cacgacgagc cgcgcctcca gcggaacggg ctgtccccga tccacgaacc ggaagtgcac
     2221 ggcgtagctg accccgctat cgctcatcgt ccgatagaag gtctctttcc cgatgttctg
     2281 tgcaaatttg tggaggtaga atgtcgcgct atctcccttt tccccaaccc ccgcagttcc
     2341 cgcgacccct gcggcaatag ccgccttggc actctccgcc gatacgcctt gcgcccttcc
     2401 cgcagccggc cccacgccag ccaaaacgcc ggccaccgcc agccccaagc aatatttttt
     2461 cataacagaa aatatacaac aaattcctga cggccgcgat ctgccccgcg aaaacagccc
     2521 cgcaaaaaca cccctacaca taaattccta ccc
//
LOCUS       DATGKE010000606         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_866571, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000606 DATGKE010000000
VERSION     DATGKE010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866571"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..904
                     /locus_tag="VKU83_10295"
     CDS             221..904
                     /locus_tag="VKU83_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93992.1"
                     /translation="MRKTAMVLLVVLLPAVSVFAQTAVELVPQVGYSFPERNDFYSIY
                     GRVAGGVNVGGAINFNVNRDFGVEVLYNHVASSSGVYYYGYDGGNQINGGNLTQDYIM
                     AGPVFSGNIPNSTVRPFLGLLMGAAILTPDPGFQSNSYFSVGFQVGTNIYISPNVGIQ
                     LKAQLLSPVAAAGGAFYYNDYYGGGINPNQSICQFTVGGGLIIGLGRVLPKPHYVYRR
                     PRPRYYRYY"
     gene            1186..>2163
                     /locus_tag="VKU83_10300"
     CDS             1186..>2163
                     /locus_tag="VKU83_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLI93993.1"
                     /translation="MEFQPDELTQQVGRTARDFANQAIRPHVMQWDEKQEFPRELFRE
                     LGRLGMMGVVVPETYGGSGLSYTEYVTVIEEIAKVCGGIGLSLAAHNSLCTGHILAFG
                     SEEQKMRFLPRLAGGEALGAWGLTEAGTGSDAGNMRCTATRDGDEWVINGTKNWITHG
                     LSSEIAVVVARTGEPRTRNNASAFVVERGTAGFSGGKKENKLGMRASETAEMIFDQCR
                     IPAANQLGGTGDGFRQALAVLDGGRISIAALSLGIAKGALEAALQYAKERHQFDQPIA
                     QFQGISFKLADMATEIAAAELLVMQVAWKLDQGSAADQDFAMAKLFATET"
BASE COUNT          449 a          597 c          634 g          483 t
ORIGIN      
        1 ataccgtgcg cctggtttac ccacatcagc catcagctgg gcaagcatgc ggagattgaa
       61 cctacgcttc cggcgaatcg tcacctggct tatgatgggc tggttctcga atgaggaacc
      121 actttttgca tttcattaac atcttattag catgaagccg gtcgaccggg cgttaaacct
      181 ttggcaaggt tttatgtcta tttacttata aaacgtaagt atgagaaaga cagcaatggt
      241 actattggtt gttttattgc cggcagtatc cgtatttgcg cagactgccg tggagctggt
      301 cccgcaggtt ggttattcct ttcccgaaag aaacgatttt tacagcatct atggtcgtgt
      361 agccggcggg gtgaacgtcg ggggcgccat caattttaac gtgaacaggg acttcggtgt
      421 cgaggttctt tacaatcatg tcgcttcctc gtcgggcgtc tattattacg ggtatgacgg
      481 cggcaaccag atcaacggcg gaaatctgac ccaggattac atcatggcgg ggccggtctt
      541 ttccggcaat atcccgaatt cgaccgtgcg gcctttcctc ggcctgctga tgggtgcggc
      601 cattctgacg cccgatccgg gcttccagtc gaacagttat ttttccgtgg gcttccaggt
      661 cgggacaaat atctacatca gcccgaacgt gggcattcag ctgaaagcgc agctgctttc
      721 gccggtggct gccgcagggg gtgcgttcta ctataacgac tactatggcg gcggcatcaa
      781 cccgaaccaa tccatctgcc agtttacggt cggcggaggc ctgatcatcg gtctcggccg
      841 cgtcttgccg aagccccatt atgtgtatcg ccgcccgagg ccccggtatt accgctacta
      901 ttgattttga tccatagctc aacaagtcag ccgctcgcct gtcgcaggag cggctttttt
      961 ttcgcgccgt aaactaacgg ccgttagttt gtctcgaaaa aattatttaa ctttgtatcc
     1021 attcatgccc ggcgtaacgt ttctttaccg gccgcgagcc ggggacgcgc aggccagagg
     1081 tccgaataat cgttgtttgc cagtgttgcc aggcaatagc ctccggttgt tgcctggttc
     1141 ttttattcgc cggaaacaag ccggtacgtt taaatcaacc catacatgga atttcaaccg
     1201 gatgaattaa cccaacaggt agggcgcacc gcccgcgatt tcgctaacca ggccatccgg
     1261 ccgcacgtta tgcaatggga cgagaaacag gagtttccgc gcgagctgtt cagggaactg
     1321 ggccggctcg gcatgatggg cgtcgtcgta cccgagacct atggcggatc gggcttatcg
     1381 tacacggaat acgtgaccgt catcgaggag atcgcaaagg tctgcggcgg gatcgggctg
     1441 agtctcgctg cgcacaattc gctttgtacc ggccatatcc ttgccttcgg atcggaggag
     1501 caaaaaatgc ggttccttcc ccggctggcg ggtggggagg ccctgggcgc ctgggggctt
     1561 accgaagcgg gtaccggcag cgacgccgga aacatgcggt gcacggcgac gcgggatggc
     1621 gatgagtggg tgatcaacgg gaccaagaac tggatcacgc atggcctcag ttccgaaatt
     1681 gccgtcgtgg tcgccaggac cggcgagccc cgcacccgca acaacgcaag cgcgtttgtt
     1741 gtcgaaaggg gtaccgcggg tttcagcggt gggaaaaagg agaacaaact cggcatgcgc
     1801 gccagcgaaa cggcagaaat gatcttcgat caatgtcgaa tcccggccgc caaccaactc
     1861 ggcggcacgg gggatggttt tcgccaggcg ctggcggtgc tggacggggg ccggatctct
     1921 atcgcagcgc tttcactcgg cattgccaag ggggcgcttg aggcggccct tcaatatgca
     1981 aaggagcgcc accaattcga ccagcccatc gcccaatttc aaggtatttc cttcaagctc
     2041 gccgacatgg caaccgagat cgccgccgcg gaactgctgg tcatgcaggt ggcctggaag
     2101 ctggaccagg gcagcgccgc cgatcaggat ttcgccatgg ccaagctctt cgccaccgag
     2161 acg
//
LOCUS       DATGKE010000607         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_773096, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000607 DATGKE010000000
VERSION     DATGKE010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773096"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1381
                     /locus_tag="VKU83_10305"
     CDS             197..1381
                     /locus_tag="VKU83_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93994.1"
                     /translation="MAEKLVKRRCVISIGDYESIDVEQLFANFQRGLHRFGKTWNVDA
                     RYSKLKMEADGAVAVWNIETKTSNWQVKTVFRLLNWSDIVKKDFRRWSLNRVWRAIKA
                     LTNFAVSGTCWRYFRLNWRFGLFFLYPALTVFLFTIFALWLTVLLGNFEVPYTGFFGF
                     VIGLGLFAAFIKWVDPVVLPRVVDMWIFMYELVHLERTSLAERLGVFSQDIVAQLQSN
                     DFDEVLIIGHGIGAVLQPIIVDRAFWALPEFGKDGRSVSLLSLGSLLLAVGLHPEGDW
                     VVGPVSRVARDRWVKWVEYQAEKGVIGFPGSNPVTELISDYHKPVLQNIQIKDMTDAK
                     RSFSGSIYQNHRQLVRANSKRYFYDYFMTFCGPFTLSMRMEHPELMVEAFHPDGRLIV
                     GL"
     gene            complement(1398..1697)
                     /locus_tag="VKU83_10310"
     CDS             complement(1398..1697)
                     /locus_tag="VKU83_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93995.1"
                     /translation="MISLQSERERPMSNFVKLFFGFVALGLLAILAVPGPASAITAEL
                     AKKCQAMAAKAHPMALPGSKVGTAKAERDYFGACIKNNGTMPDSNTQNTTTPAAK"
     gene            complement(1951..>2520)
                     /locus_tag="VKU83_10315"
     CDS             complement(1951..>2520)
                     /locus_tag="VKU83_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI93996.1"
                     /translation="PLPFFPTDKTEIDYASAQRDRAAIVASLGWPRGDLWADYALSAD
                     CRFLNDVPASTTIDRTEPVSEHALALAPYDARLWLLLSAINAQSGWKDDKTLAQLKMS
                     YYTAPNDIRLIPLRISIALQSQAIGDKELQELVEHEIRTIIMRKPELKPVIATAYRGA
                     SAAGRAFVEEKLAVLDRKFLTDLQTISTR"
BASE COUNT          572 a          590 c          680 g          678 t
ORIGIN      
        1 tgccgcaggg catgccctaa agaaggctct cacaagacat ggctcggcgg cgatcgttca
       61 cgcccctgaa cttcgccaac aactgcatag aggctccaag tgaatgccgt ctgtgctgcc
      121 gcaggaaggg ttcgggctgc cattgatgct tctaccattg tccacgaaat ttccatcgaa
      181 atgaccggtg aatcacttgg ctgaaaagct agtaaaaagg cgatgcgtta tttcgattgg
      241 agattatgag tcaatcgatg tcgaacagct gttcgcaaac ttccaaaggg gactgcaccg
      301 ctttgggaaa acctggaatg tcgatgcgag atactccaaa ttaaaaatgg aggcagatgg
      361 cgctgtggcc gtttggaata tcgaaacgaa aacatcaaat tggcaggtaa aaacagtatt
      421 tcgactattg aactggtcgg acatcgtcaa aaaggatttt cgtcgctgga gtcttaatcg
      481 tgtttggcgg gctatcaaag ccctcaccaa ttttgccgtt tcagggactt gctggcggta
      541 tttccgttta aattggcgct ttggtctatt ctttctctat ccagctttga ctgtcttttt
      601 atttacgatc tttgcacttt ggctcacagt gctacttggc aattttgagg tgccttacac
      661 ggggttcttt ggctttgtaa ttggccttgg gctatttgct gcattcatta aatgggtcga
      721 tccggttgtt cttccgcgtg ttgttgatat gtggatcttt atgtacgaac tcgttcatct
      781 tgagcgaacg agtcttgccg agcgattggg tgtttttagc caggacattg ttgcgcaact
      841 gcaatcaaat gacttcgatg aagtcctaat tatcggccac ggaattgggg cggtgctgca
      901 accgatcatc gtcgaccgtg cgttttgggc actgcctgag tttggcaagg atggacgctc
      961 tgtcagtttg ttatcattag gctcactgct gctggcggta ggattacatc ctgaagggga
     1021 ctgggttgtc gggcccgtgt cacgggtcgc gcgtgaccgg tgggtcaaat gggtggaata
     1081 tcaagccgaa aaaggcgtca ttggctttcc cggaagcaac cctgtcaccg aattgatcag
     1141 cgattaccac aaaccggtac tacaaaacat tcagatcaag gacatgactg acgctaaaag
     1201 atctttttca ggatcgattt atcaaaatca tcgccagttg gttcgagcca acagcaaaag
     1261 gtatttctac gactacttca tgaccttctg cggtccgttt acactctcga tgcgaatgga
     1321 gcatccagag ctcatggtcg aagcgtttca tccggatggt cgactgattg tcggactata
     1381 gccgattgta atttggctca ctttgctgcg ggagttgtgg tgttttgtgt gttgctgtcg
     1441 ggcatcgtgc cattattttt aatgcatgca ccaaagtagt cccgctccgc tttagcggtt
     1501 cccactttgc tgcccggtaa cgccattgga tgagccttgg ccgccatggc ctgacatttt
     1561 ttggccagtt cagctgtaat cgccgatgcg gggccaggaa cggctaaaat cgccaggagg
     1621 cccagtgcga cgaacccgaa aaagagtttt acaaagttac tcatgggccg ctcccgttcc
     1681 gactggaggc tgatcacata atggctaaat atgtttacct ggcaaggctc aattgaatgc
     1741 cgttcacgga gcgcgcatct ttgcctgatc ccatattgcg cgtatgggct gcatcatcgc
     1801 ggtttgggcg aagggcggca ggtcatacat tgccagcagc gttcttagca cgccgtaatg
     1861 tgagatgttt tcagcgtatt tgtcgggctg cacgcgggcg ctgttgatta ctgtcggggt
     1921 gcggtttttg gtgaacggcc aaaagtcgaa ttatcgggtg ctgattgtct gtaagtcggt
     1981 caaaaacttt cgatccaata cagctagttt ttcttcgaca aaagcacgac cggccgcgga
     2041 agctccgcga taagccgtcg cgatgacggg cttgagttcc ggtttacgca tgatgattgt
     2101 acgtatctcg tgctctacca actcctgcaa ctccttgtcg ccgatagcct gcgattgcag
     2161 tgcgatcgag atgcgcagtg gaataagacg aatatcattt ggcgcggtgt aataggacat
     2221 tttaagttgc gccagggttt tgtcgtcttt ccagccagat tgcgcattga ttgcggacaa
     2281 caatagccaa aggcgagcgt catagggcgc gagggctaat gcgtgttccg aaacaggttc
     2341 ggtcctatca attgtggttg atgcaggaac atcattgaga aatctgcaat cagcgcttag
     2401 cgcataatcc gcccacagat ctccgcgcgg ccaaccgagc gaggcgacaa tagccgccct
     2461 atcacgctgt gccgacgcat agtcaatttc ggtcttgtcg gtgggaaaaa aaggtaacgg
//
LOCUS       DATGKE010000608         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_555612, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000608 DATGKE010000000
VERSION     DATGKE010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555612"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..930
                     /gene="phoU"
                     /locus_tag="VKU83_10320"
     CDS             265..930
                     /gene="phoU"
                     /locus_tag="VKU83_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLI93997.1"
                     /translation="MAQIETELQLLKSEAINMWSLVNSQLNKARLAFINFDKDLAREV
                     VLKEKRVNGSELKIDRDCENIFALFCPVAIDLRFLLAVLKINTNLERIGDIAEGIAKY
                     VIDADAPFTQQLLDASRILPMYEESVKMLEDTQEAFEKEDTILARGVFKKDDFLDDVN
                     RQSHRVLEKHLKDHPQDTSQALWILSIIRKLERVGDQAKNIAEEIIFYLEAKVLKHRN
                     KFV"
     gene            complement(1117..2277)
                     /locus_tag="VKU83_10325"
     CDS             complement(1117..2277)
                     /locus_tag="VKU83_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLI93998.1"
                     /translation="MRPPISHPGHNTHCADHLRRSALPPRRIVYGAAILLLTACGHSA
                     APGGNAPAAGLQDTVQAFLLTTDTAKKSVDLPGELEPYLQTDLFAKVQGYVRDMKVDI
                     GDRVRKGQTLAVIEAPEVNTQVTQSLAAIAAARAKYTASADKYRRLYQASLSTSPGIV
                     APVDLISARDQMEADSAAYEALQQQSKAYREISGYLYLTAPFDGVIIARKADPGALVS
                     ASSMLLTVQDNNTLRLRVAVPESYVDAATGGRLSFSVDANPEKRFTGVLTRKSGSIDP
                     VTRTELWEFDVDNRGHQLIAGAFVYAHLQVERNRPSFIVPPSAIATTLERKFVIRVHD
                     GKAQWIDVRQGMTTDNGVEVFADLHPGDTLLAKATDERKPGTSAVWKVTALK"
     gene            complement(2283..>2971)
                     /locus_tag="VKU83_10330"
     CDS             complement(2283..>2971)
                     /locus_tag="VKU83_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI93999.1"
                     /translation="YITITANIAQKDRGAVYSKIRQVIAGMGKLPNGARILLRGQSEL
                     LEQTLQSLRFGLLIAIVVIFLAMAVFFQSFRVALATLAVIPAVIAGALFLLYVTGNTL
                     NIQSYMGAIMAVGVAIANSVLFITNAEQLRRSAVAAVHLEAAGHRLRPILMTTVAMIA
                     GMIPMALGLTEGGEQTAPLGIAVIGGLIFSALGVLFFLPHIYQWLAGRRTYQPVSLYP
                     GDLHSEQNTP"
BASE COUNT          666 a          798 c          901 g          606 t
ORIGIN      
        1 ttatcgtcgt cacccacaac atgcagcagg cggcgcgggt cagcgataag accgcctttt
       61 tttacatggg cgagcttgtg gaatacgacg acacgaaggt gatgttcacc aacccgaaag
      121 acagtaggac acaaaactat attacaggtc gttttagctg acagcccgga ccacaggtcc
      181 gccgcgcccg acggcccatc aacagcgaat caccggggat gggttaaatt tcggcagaaa
      241 ccatccccgg tgtaacttta aataatggca caaatagaaa ctgaactcca gctcctgaaa
      301 tccgaggcca tcaatatgtg gtcgctcgtc aattcccagc tgaacaaagc gaggctggcc
      361 ttcattaatt tcgacaagga ccttgcccgg gaagtagtgt taaaggaaaa gcgcgtaaac
      421 ggaagcgagt taaagatcga ccgtgactgc gagaatattt ttgcgctctt ctgtccggtc
      481 gccatcgacc tcaggttcct gctggccgtg cttaagatta ataccaacct ggaacggatc
      541 ggtgacattg cggaaggcat tgccaaatat gtgatcgatg ccgacgcgcc cttcacccag
      601 caattgctgg acgcgtcccg tatcctgccg atgtacgagg agtcggtgaa aatgctggag
      661 gatacccagg aagcctttga aaaggaggat accatcctgg cccgcggcgt cttcaaaaag
      721 gacgacttcc tcgatgatgt caaccggcag tcgcaccggg tgctcgaaaa acacctgaaa
      781 gatcaccccc aggatacctc ccaggcgctg tggatcctgt ccatcatccg gaaactggaa
      841 agggtgggcg atcaggccaa gaacatcgcc gaggagatca tcttctatct ggaggccaag
      901 gtgctgaaac accggaataa atttgtctga cagcgccggg cccgcctaat ataatatagg
      961 ggtaacgtta gcgtaaactc tgcataatac ggcagggtta tttttatgcc tgtaacggat
     1021 gggcaaccgg catctccgcc tttaatcgat ggtttatata tgtagagttg aatattctca
     1081 tgcagaagcc acttcatttc catgacggtg gctttcttat ttcaacgcgg tgactttcca
     1141 gacggccgag gttccgggtt tgcgctcgtc ggtggccttc gccaacaggg tatcgcccgg
     1201 atgcaagtcc gcaaaaacct caaccccgtt gtcggtcgtc attccctgcc ggacatcgat
     1261 ccattgcgct tttccgtcgt gtacccggat cacaaacttt cgttccagcg tggttgcaat
     1321 cgccgaaggc ggtacaatga aggatggccg gttgcgttcc acctgcaggt gcgcatagac
     1381 gaatgcgccg gcgatcagct ggtggccgcg gttgtcgaca tcaaattccc agagctccgt
     1441 gcgcgtgacg ggatcgatcg accccgattt gcgcgtcaat acgccggtga agcgtttttc
     1501 cggattggca tccacgctga agctaagccg gccccctgtg gcggcatcca cgtaggattc
     1561 gggtacggcg acgcgtagcc gcagggtgtt gttatcctgc acggtaagca gcatgctgct
     1621 cgcgctgacg agtgctcccg gatcggcctt gcgggcgatg atgacgccgt caaacggagc
     1681 ggtgagatat aaataacccg agatttccct gtaggctttc gattgctgct gcaacgcttc
     1741 ataggcagcg ctatccgctt ccatttgatc gcgcgcactg atcagatcga ccggggcgac
     1801 gatgccgggc gacgtggaga gagaggcctg gtaaagccgc cggtatttat cggcgctcgc
     1861 cgtgtatttg gcccgggcgg cggcgatcgc agccaaggac tgggtcacct gtgtattgac
     1921 ttccggcgct tcgatgacag ccagggtctg gcctttccgg acccgatcgc cgatatcgac
     1981 cttcatgtcg cgaacgtagc cctgcacttt tgcgaagagg tcggtctgga gatagggctc
     2041 gagctcaccg ggaagatcca cggatttttt ggcggtatcg gtcgtgagca gaaaagcctg
     2101 aacggtgtcc tggagcccgg cagccggagc gttgcccccg ggggccgcgg aatggccgca
     2161 ggccgtgagc aggaggatgg ctgcgccgta aacgatcctg cggggcggaa gcgcgctgcg
     2221 gcgcaggtga tctgcgcagt gggtgttatg accgggatgg gagataggag ggcgcatggg
     2281 gatcatggtg tattttgttc ggaatggaga tcgccgggat ataatgagac gggctggtac
     2341 gttcggcggc cggccagcca ttgataaata tgcggcagaa aaaacaatac gccgagggcg
     2401 gaaaagatca ggccgccgat cacggctatt ccaagcggag ccgtctgctc gccgccttcg
     2461 gtgaggccca gggccatggg aatcatgccg gcgatcatcg ccacggtcgt catcaggatg
     2521 ggccgaagcc gatgacccgc agcctccagg tgaacggccg cgaccgcgga acggcgaagc
     2581 tgctcagcat tggtaatgaa caggacggag ttggcaatgg cgacacccac ggccatgatc
     2641 gcgcccatat aggattgaat gttcagcgta ttgcccgtga cgtacagcag gaacaaggcg
     2701 ccggcaatta ccgcgggaat cactgccagg gtcgcaaggg ctacgcgaaa ggattggaag
     2761 aagacggcca tggccaaaaa gatgaccacg atggcgatga gaaggccgaa gcggaggctc
     2821 tgcagcgttt gctccaggag ctcgctttgt cccctgagga gaatgcgggc gccgttcggc
     2881 agtttgccca tcccggcaat gacctgccgg attttcgaat agacagcgcc ccggtctttt
     2941 tgggcgatgt tcgcggtgat ggtgatatag c
//
LOCUS       DATGKE010000609         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1282813, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000609 DATGKE010000000
VERSION     DATGKE010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282813"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VKU83_10335"
     CDS             complement(<1..499)
                     /locus_tag="VKU83_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI94000.1"
                     /translation="MRRFIISILLFFVTGTLIAQTRISGTVRDSKGHPIHGASIAVKN
                     SYDGGVTDSLGAFHFTTSETGTDTITVSNIGYDPLERPVTLNGTPVVLHLVLKEQLSE
                     LKAVTITAGSFSAGDSKRGAVLSSLDVATTAGSNADITAALKTLPGAQQVGEQEGLFI
                     RGGAGY"
     gene            complement(1025..>2350)
                     /gene="atpA"
                     /locus_tag="VKU83_10340"
     CDS             complement(1025..>2350)
                     /gene="atpA"
                     /locus_tag="VKU83_10340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLI94001.1"
                     /translation="VKRTGAIVEVPVGKGMLGRVVDPLGNPIDGKGELTNIAERRRVD
                     VKAPGIIPRRSVHEPMQTGLKAIDALIPIGRGQRELIIGDRQTGKTAVAIDTILNQKA
                     VNAGGDESSKLYCIYVAIGQKRSTVAQIVKTLADAGALEYTIIVTATASEPAPLQFLA
                     PFSGCAMGEWFRDNGMHALIIYDDLSKQAVAYREVSLLLRRPPGREAYPGDVFYLHSR
                     LLERAAKLITDDAVAKKMNDLPDSIRHLVKGGGSLTALPIIETQAGDVSAYIPTNVIS
                     ITDGQIFLEGNLFNSGIRPAINVGISVSRVGGNAQIKSMKKVAGTLKLDQALYREMEA
                     FSKFGGDLDAATKMVIDKGARNVEILKQPQYSPFTVEHQVAIIYLGTQGLLRQVPVRR
                     IREFEEHFLMEMDNKLPDVLAEFKKGNLPDDGIKRMVDLAGSLIPQYVK"
BASE COUNT          490 a          632 c          706 g          522 t
ORIGIN      
        1 cgtagccggc gccgccccgg atgaagaggc cttcctgctc tcccacctgc tgggcaccgg
       61 ggagggtctt cagcgcggct gtgatatccg cgttcgaacc cgcggtcgtg gcgacgtcca
      121 gcgagctcag gaccgctcca cgtttcgagt cgccggccga aaaggagccg gcggtgatgg
      181 tcactgcctt cagttcgctc agctgctctt tcagaacaag gtggaggacg accggcgtgc
      241 cgttgagcgt caccggcctt tcaagcggat cataaccgat attactgacc gtaatggtat
      301 cggtcccggt ctcactcgta gtgaagtgga acgcccctaa tgagtccgtg accccgccgt
      361 catagctgtt tttgacagcg atactggcgc cgtggatcgg gtggcctttg ctgtccctga
      421 ctgtccccga gatgcgcgtc tgcgcaatca aagtgccggt aacaaaaaac agcaagatgg
      481 agataatgaa gcgtctcatg gcggcgtaaa ttcgttgtaa agatagacta gtttataata
      541 taaaccaaat tattctggag attattccgg gactgcgccg gcagacccgg ccggacaaaa
      601 aaagccaggc ttagaaaagc ccggcggttg ttgatcgtac cctttaaaac taaaatctta
      661 gcggatataa taattttatg ttgacgcaga cgcccctacc ctttgcttag tgcctggcaa
      721 tcaacaacat atctttttat tttgatcaga tggacaaggg ttatacccaa aagaagaaga
      781 cgggtcttga tacggacaca tttgcttcat agtctgtcga tgtacggaaa agaagaaaaa
      841 ggattggaaa taaatcgaac ggatgttttt cgggagcttg gaggggagtg tctttgacga
      901 ccctgtaaag attgccaaaa gtttttaaat ctgcaagcgt ttatccttaa attttttgtt
      961 gcccggggca aaaaggaacg cccggcctca gccgggcgcc aaaacactaa cgaatatgct
     1021 aaacctattt gacgtattgc gggatcaggc ttcctgccag gtccaccatt cgcttgatgc
     1081 catcgtcggg gaggtttcct ttcttgaatt ccgccaggac atcggggagc ttgttgtcca
     1141 tctccatgag aaaatgttct tcgaattccc tgatccttct gaccggcacc tggcgcagca
     1201 aaccctgggt accgaggtaa atgatcgcta cctggtgctc gaccgtgaaa ggcgagtatt
     1261 gcggctgttt gaggatctcc acgttacggg cgcctttgtc gatgaccatt ttcgtcgctg
     1321 catccaggtc gccgccaaac ttggaaaaag cttccatctc gcggtatagg gcctggtcca
     1381 gtttgagggt gccggccacc ttcttcatcg atttgatctg cgcattccca ccgacacgac
     1441 taaccgaaat gccgacattg atcgcgggcc ggatccctga gttgaacagg tttccttcca
     1501 ggaagatctg cccgtcggtg atggagatta cattcgtggg gatataggcg gaaacgtcac
     1561 ccgcctgcgt ctcgatgatc ggcagggcgg tcagcgaacc gccgccttta acgagatggc
     1621 ggatggaatc aggcagatcg ttcatctttt tggccaccgc atcatcggtg atgagcttgg
     1681 cggcgcgctc gagaagccgg ctatggagat agaaaacgtc cccgggatag gcttcgcggc
     1741 cgggcggacg gcgcaggagc agggacactt cgcggtacgc gacggcctgc ttggaaaggt
     1801 catcatagat gatcagggcg tgcatgccgt tgtcgcggaa ccactcgccc atggcgcagc
     1861 cggagaaggg cgccaggaac tgcagcggcg ccggctcgga cgcggtggcg gtgacgatga
     1921 tggtgtattc cagcgcgccg gcgtcggcga gggtcttgac gatctgcgcc acggtcgaac
     1981 gcttctggcc gatggcgacg tagatgcaat agagcttgga gctctcgtcg ccaccggcgt
     2041 tgaccgcctt ttgattgagg atggtgtcga tcgccacggc ggtcttgccg gtctggcggt
     2101 cgccgatgat cagctcgcgc tggccgcggc cgatcgggat caaggcgtcg atggccttga
     2161 ggccggtctg catcggctcg tgcacggagc ggcgcgggat gatgcccggc gccttgacgt
     2221 cgacgcggcg gcgttcggcg atgttggtga gttcgccctt gccgtcgatg ggattgccca
     2281 ggggatcgac gacgcggccc agcatgcctt tgcccaccgg cacttccaca atggcgccgg
     2341 tgcgcttgac
//
LOCUS       DATGKE010000610         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1281286, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000610 DATGKE010000000
VERSION     DATGKE010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281286"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /gene="accB"
                     /locus_tag="VKU83_10345"
     CDS             <1..329
                     /gene="accB"
                     /locus_tag="VKU83_10345"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HLI94002.1"
                     /translation="AAPAPAPHTPVAAAAPEKPARAAEPPAANTVTIKSPMIGTFYRS
                     PSPDKPSFVNVGDDVEPGKVVCIIEAMKLFNEIESEVKGRIVKVLVEDASPVEYDQPL
                     FLVEPA"
     gene            384..1721
                     /gene="accC"
                     /locus_tag="VKU83_10350"
     CDS             384..1721
                     /gene="accC"
                     /locus_tag="VKU83_10350"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLI94003.1"
                     /translation="MFKKVLIANRGEIALRVIRTCREMGIKTVAVYSTADKESLHVRF
                     ADEAVCIGKPASFESYLNIPHIMAAAEITNADAIHPGYGFLAENARFAEICGEHDIKF
                     IGPTPAMINSMGDKITAKETMIKAGVPVVPGGEGLLGSLDEARGLAREVGYPVILKAT
                     AGGGGKGMRVVWEESEMERAYNTARAEAGAAFKNDGVYMEKFVEEPRHIEIQVAGDQY
                     GRVCHLSERDCSIQRRHQKLVEESPSPFMTEDLRERMGAAAIKAASAINYESVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHCVTEEVTNFDLIKEQIKIANGEAISGLNYEPQGHAIE
                     CRINAEDPYNDFRPSPGRITVLHQPGGHGIRVDSHAYAGYTIPPYYDSMIAKIIAVAR
                     TREEAINTMHRALSEYVIEGIKTTIPFHLQLMKDERFRSGDFNTKFLDGFKML"
     gene            complement(1767..>3023)
                     /locus_tag="VKU83_10355"
     CDS             complement(1767..>3023)
                     /locus_tag="VKU83_10355"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLI94004.1"
                     /translation="VPLPFVMKNFEIPIIYRSPLVAAIKAERRRQDRMKRDFSPTRLD
                     FGRLQIFLARHFGFCYGVENAIEISFRTIDQNPGRRIFLLSEMIHNPHVNADLQSRGV
                     QFLQDTRGNQLIPFESLTADDIVIIPAFGTTLEIQARLRQIGSRTEQYDTTCPFVEKV
                     WNRSAQIAEKKYSIVLHGKPGHEETRATFSHAAAVTPTVVIKDMDEARALAGYINGTR
                     PAGDFYPEFRGRYSPGFDVKKDLTRIGVVNQTTMLASDTQEIADFLRQTMVETYGTAA
                     GTIEQHFADTRDTLCYATLDNQSAVQTLLQMDADLAIVVGGYNSSNTSHLVELCEQQV
                     PTYFVRSDDNLLSPTEISHYLFHEKQELTTKDWLPAHKPEPVRILLTSGASCPDALVE
                     AVICRLAAFFDAEDQIPALIARFAAS"
BASE COUNT          678 a          792 c          932 g          621 t
ORIGIN      
        1 cggcagcccc cgcgccggct ccccatacgc ctgttgccgc ggcggcgccc gaaaaaccgg
       61 cccgggcggc ggagccgccc gcagccaata cggttaccat caagagcccg atgatcggca
      121 cgttttatcg cagcccctcg ccagataagc cctcttttgt gaatgtcggc gacgacgtcg
      181 aaccaggcaa agtggtctgc atcatcgaag ccatgaagct gtttaacgag atcgaaagcg
      241 aagtcaaagg caggattgta aaagtcctgg tagaggatgc ctcccccgtt gaatatgatc
      301 agcccctgtt cctggtagaa cccgcctgat ccatcctgaa gcaggcggag caccgcaggc
      361 attaacggtc aataaatcta agaatgttta aaaaagtact gattgccaat cgaggagaga
      421 ttgccctgag ggtcatccgc acctgccggg aaatgggtat caagacggtt gcggtctact
      481 ccacagctga taaagaaagt ctccatgtac ggtttgccga tgaagcggtt tgcatcggga
      541 agccggcaag tttcgagtcc tacctcaata ttccacacat catggcggca gcggagatca
      601 ccaatgccga cgctatccat cccggttacg ggttcctggc tgaaaacgca aggtttgcgg
      661 agatctgcgg cgagcatgac atcaaattca tcggtccaac gccggcgatg atcaactcga
      721 tgggcgataa gatcacagcg aaggaaacga tgatcaaagc cggcgtgccg gtcgtgccgg
      781 gtggcgaagg cttgctggga agcctggacg aggccagggg gcttgccagg gaggtcggat
      841 acccggtgat cctgaaggca accgccggtg gcgggggaaa gggcatgcgc gtcgtttggg
      901 aggagtccga gatggagcgc gcatacaaca ctgcgagggc cgaggcgggg gcagcattta
      961 agaacgatgg cgtttatatg gagaagtttg tggaggagcc acggcatatc gagatccagg
     1021 tcgccggtga tcaatacggc cgggtttgcc atttgagcga gcgcgattgc agcatccagc
     1081 gccgccatca gaaactggtg gaggaatcgc cgtcgccgtt tatgacggag gacctgcggg
     1141 agcggatggg cgcagcggcg atcaaggcgg cctccgcgat caattatgag agtgtgggca
     1201 ccattgagtt cctcgtggat aagcaccgga atttctactt tatggaaatg aacacgcgga
     1261 tccaggtgga acactgcgtg acggaagagg tgacaaattt cgatcttatc aaggagcaga
     1321 taaagatcgc taacggcgag gccatcagcg gcctgaatta tgagccgcaa gggcatgcca
     1381 ttgaatgccg gatcaatgcc gaagatccct ataacgactt ccggccatct cccggccgga
     1441 ttacagtctt gcaccagccg gggggacatg ggatccgggt ggacagccac gcctatgccg
     1501 ggtacactat tcctccttac tacgactcga tgatcgccaa gatcattgcg gttgcaagga
     1561 cgagggaaga ggcgatcaac acgatgcacc gggccttgag cgagtatgtg atcgagggca
     1621 tcaagactac tatccccttc cacttgcaat tgatgaagga cgaacgtttc cggagcggcg
     1681 acttcaacac aaaattcctg gatggtttta agatgctgta acgccgggcc ggctttcggg
     1741 cagcatggcc gcggctacgg gagcgttcag gaggcggcaa acctggcgat cagggccggg
     1801 atttgatctt ccgcatcgaa gaaggcggcc agccggcaga tcacggcttc caccagcgcg
     1861 tccgggcagg atgcgccgct ggtcagcagg atgcgcaccg gttctggttt atgtgcgggc
     1921 agccaatcct tcgtggtgag ttcctgtttt tcatgaaaaa gataatggct gatctcggtt
     1981 ggcgacagca aattgtcgtc cgaacggaca aagtacgtcg ggacctgctg ctcacacaac
     2041 tctacgaggt gcgaagtatt ggaggagttg tatccgccca cgacaatggc gaggtcggcg
     2101 tccatctgga gcagcgtctg caccgctgat tggttatcga gggtcgcata gcaaagcgta
     2161 tcccgcgtgt cggcaaagtg ctgctcgatc gtcccggcag ccgtgccata ggtctctacc
     2221 atggtttggc ggagaaagtc ggcgatttcc tgggtatcgc tggcaagcat ggttgtctgg
     2281 ttcacgacgc cgatacgggt gaggtctttc ttaacgtcaa atccggggct gtaccggccg
     2341 cggaattcag gatagaagtc gcccgccggt ctggtgccgt taatataacc tgcaagggca
     2401 cgcgcttcat ccatatcttt aatgacaacc gttggcgtca cggccgcggc atgggagaag
     2461 gtggcgcggg tctcctcgtg accgggtttg ccatgcaata cgatactata ctttttttcc
     2521 gcgatctggg cgctccggtt ccagaccttt tccacgaagg ggcaggtcgt atcgtactgt
     2581 tccgtccggc tgccgatctg ccttagccgc gcttgtattt caagcgtcgt gccgaaggct
     2641 gggatgatca cgatatcgtc cgcggtcagg ctttcgaaag ggatgagttg attgccccgc
     2701 gtatcctgca ggaattgtac gccgcgggat tgcaggtcgg cgttgacgtg agggttgtgg
     2761 atcatctcgc tgagcagaaa tatgcgccgg ccggggtttt gatcgatggt ccggaacgag
     2821 atctcgatcg cattctcgac gccgtagcaa aaaccaaaat gccgggcgag gaatatctgg
     2881 agccggccga agtccagtcg cgtaggactg aaatcccgct tcattctatc ctgtcgccgg
     2941 cgctctgcct tgatggcagc gaccagcggg ctgcgataaa taatcgggat ctcaaaattt
     3001 ttcataacga atggcaaagg tac
//
LOCUS       DATGKE010000611         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_762064, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000611 DATGKE010000000
VERSION     DATGKE010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762064"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1659
                     /locus_tag="VKU83_10360"
     CDS             166..1659
                     /locus_tag="VKU83_10360"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HLI94005.1"
                     /translation="MKFKIGIAVLVAIAATHFGNLAFAQYRMEKDLLGEKQIPSGAYY
                     GVQTARALENFQVSGITTKFYPDYVRAYAMVKLAAARANAQIGRMSNEKLQAIEKACQ
                     AVIDGKYLEHFLVDLYQGGAGTSANMNANEVLANIALEMSGHKKGEYQFIEPHDDLNM
                     GQSTNDVYPTAIHVALLLHNDKLVKEAKALSAAFHKKGDEFDKLLKMGRTEGQDAVPM
                     TLGQEFHAFGGQIDAAIEGLVKSEAYLYEENMGATAIGTGITASPGYAEKCAAELAKI
                     VGKPMHLSANLIAATSSMQAFVMYSAGLKNLAVTLSKISSDLIFLATGPRTGIFEINL
                     PALQPGSSIMPGKVNPVMAELMNEVCYKAIGNDATVTIASRDGLLQLNAYEPVVAIAI
                     METQALFYKTMPLFRTQCIDGITANQRILDHYMERSVGIVTALNPVLGYDKTTELAKE
                     ALQTDKGILELIREKKLLTEQQIKDLLDPAKLTSPTGPTGPVKTKTN"
     gene            1682..>2101
                     /locus_tag="VKU83_10365"
     CDS             1682..>2101
                     /locus_tag="VKU83_10365"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HLI94006.1"
                     /translation="MKKFNISLYLLLALAILISGISCRSRSEDSPVSDSTANAPQQAI
                     PVGERNGSLPNITILATGGTIAGTAASSTQAGYTSGMVTIDAMVNAVPDAKKIANISG
                     EQVSNVGSQDMTFDIMLKLGKRINTLAQSNDLTGIVVT"
BASE COUNT          563 a          559 c          535 g          444 t
ORIGIN      
        1 gcgatggatg cgttgcatag aattatttct cctgcaaggt tgggcaatta ccccgaacaa
       61 caaaagcaac cgacttccgc tttgccccaa cttataaatc ttgtcgtgtt tgtcaccccg
      121 ttatctggta ataagatgaa attcgattct taaaacagac agtttatgaa attcaaaatt
      181 gggatcgcgg tactggtggc tattgccgct acacatttcg gaaatcttgc gtttgctcag
      241 tatagaatgg aaaaggatct gctgggagaa aaacagatcc cttccggcgc ctattacggt
      301 gtacaaaccg ccagagcgtt ggaaaatttc caggtcagtg ggatcaccac gaaattttat
      361 ccggattatg taagggccta tgccatggta aagctggctg ccgcccgggc taacgcacag
      421 atcggcagga tgagcaatga aaagctgcag gcgatcgaaa aagcctgcca ggcggttatc
      481 gacggtaaat atctcgaaca cttcctggtt gatctgtacc agggcggcgc aggcacatcc
      541 gccaatatga acgccaatga agtgctggcc aatatagccc tcgaaatgag cgggcacaaa
      601 aagggagaat accagttcat cgaaccgcac gacgacctga acatgggcca atctaccaat
      661 gatgtctacc cgacggctat tcatgtggca ctgctgttgc acaacgataa actggttaag
      721 gaagcaaagg cgctctccgc cgcctttcat aaaaaaggcg acgaattcga taagctactc
      781 aagatgggac gaacggaagg ccaggatgcc gtaccgatga cccttggcca ggagttccat
      841 gctttcggtg gacagatcga cgccgcgatc gaaggattgg tgaagtccga ggcctacctg
      901 tatgaggaga acatgggcgc tactgcgatc ggtacgggga tcaccgcctc tcccggctat
      961 gccgaaaaat gcgccgccga gctggcaaag atcgttggta agccaatgca cttaagcgcg
     1021 aacctcattg ccgccacttc cagcatgcag gcctttgtca tgtattcggc cggcttgaag
     1081 aatcttgccg tcaccctgtc gaagatctcg agcgatctga tctttttggc taccgggccg
     1141 cgaaccggca ttttcgagat caatcttccg gcactccagc ccggatcttc cattatgccg
     1201 ggaaaggtca accccgtgat ggccgaactg atgaatgagg tctgctataa ggccataggc
     1261 aacgatgcca ccgtcaccat cgcctcgaga gacggactgc tgcaattgaa cgcgtatgaa
     1321 ccggtagttg ccatagccat tatggagacg caggcgcttt tttataagac aatgcccttg
     1381 ttccgcaccc aatgtatcga cggcattacc gccaatcaac gtatcctgga ccattatatg
     1441 gaacgcagtg tggggatagt cacggcgctg aacccggtgc tcggttatga taagaccacc
     1501 gagctggcga aagaagcgct gcaaaccgat aagggcatcc tggaactgat ccgcgaaaag
     1561 aaactgctga cggagcagca gatcaaggat ctcctcgatc ccgccaagtt aacatcccca
     1621 acaggaccga cagggccggt gaagacaaaa accaattgat cagcaataaa atcaaatcaa
     1681 gatgaaaaag ttcaacattt ccctttacct ccttttagcg cttgctattt tgatctccgg
     1741 catatcctgc cgttcgcgga gcgaggatag cccggtttcc gattcaacgg ccaatgcccc
     1801 gcaacaggcc attcccgtcg gcgaaaggaa cggctccctg ccgaatatca ctattctggc
     1861 aacaggcggc accattgccg gaacggcagc cagcagtaca caagccggat atacgtccgg
     1921 gatggtgacc atcgatgcca tggtgaatgc ggtgccggac gcgaaaaaga tcgcgaacat
     1981 cagcggcgaa caggtttcca atgtcggttc ccaggacatg accttcgata ttatgcttaa
     2041 gctgggcaaa cgtatcaaca cactcgctca gtccaatgac ctcaccggca tcgtagtcac
     2101 a
//
LOCUS       DATGKE010000612         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1534098, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000612 DATGKE010000000
VERSION     DATGKE010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534098"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VKU83_10370"
     CDS             <1..444
                     /locus_tag="VKU83_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94007.1"
                     /translation="QVTAADTLPAGIDFDAKLMPDSNTVWKPIVAERRGLVNLTRLYG
                     AAASKRIAWLRATIHSDDARGCTLRLGFLNEVWVWVNGRLLYLDKNYYGEPIAKQLGR
                     LSIDNSTLTVPLQKGDNEILVGVGNAFYGWGIAARLDDTNGIELR"
     gene            498..869
                     /locus_tag="VKU83_10375"
     CDS             498..869
                     /locus_tag="VKU83_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI94008.1"
                     /translation="MLADQDIMAFVPTTDPVRGRAFYEGVLGLKVTDEDPYGIMFDAN
                     GTRLRMSVVGELVPAPYTILSWVVKDVRSVVRALQGKGVAFERYGFMEQDEWGVWRSP
                     EGAQIAWFKDPDGNTLSVAEL"
     gene            complement(927..>2024)
                     /locus_tag="VKU83_10380"
     CDS             complement(927..>2024)
                     /locus_tag="VKU83_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI94009.1"
                     /translation="TIVPGFVDTHSHMWPQWGIHKNQVWKYAINLAYGVTTTRDPQTA
                     TTDILTYQDEVDAGSVFGPRIYSTGPGVGFWDYNVKDSTQAENILRQYSKYYNTHYIK
                     MYLTGNRQMREWIIMAARHQGLMPTTEGGLNLKLNLTNLIDGYPGHEHAIPVFPLYKD
                     VVKSIAFSQMCVTPTLIVAYGGPFAENYWWETENAYSDPKLQYLMPYEELASKTRRDK
                     AGWFSYDEQVFPKHAKTMNDLVMAGGLAGIGSHGEFNGIGYHWEMWAMASGGMKPMDV
                     LRVATILGATGLGLDRDLGSLKAGKLADLVVMDKNPLENIRNTNSVAYVMKNGRLYDG
                     NTCNEIYPVQRVLDRSEWHFERPANTTGVKE"
BASE COUNT          423 a          536 c          605 g          460 t
ORIGIN      
        1 caggtcacgg cagcggacac ccttccggcg gggatcgatt ttgacgcgaa attgatgccg
       61 gattccaata ccgtctggaa gccgatcgtc gcggagcgtc gcggtctggt gaacctgacg
      121 agattgtacg gcgcagccgc aagcaagcgg atagcctggc tgcgcgcgac cattcattcg
      181 gatgacgcga gaggttgcac gttgaggctg ggtttcctga atgaggtatg ggtttgggtg
      241 aatggccgtt tgctctatct cgacaagaat tactatggtg agccgatcgc caagcaactg
      301 gggaggctgt cgatcgataa cagcaccttg accgtgccgt tgcaaaaagg agataacgag
      361 atcctggttg gagtgggaaa tgctttttat ggatggggga tcgcagcgag gctggacgac
      421 acaaatggga ttgagctgcg gtgaagaagg cgaggcgcag tctggcggca actggctacc
      481 tttgagaaaa cgaatttatg ctggccgatc aggacattat ggctttcgtg ccaacgaccg
      541 atcccgtgag gggccgggct ttttatgagg gggttctggg attgaaggtt acggatgaag
      601 acccgtatgg aatcatgttc gatgcgaatg gtacccggct caggatgtcg gtggtgggag
      661 aacttgtccc cgcgccgtat acgatcctga gctgggtcgt aaaggatgtc cggtcggtgg
      721 tgagggccct gcaggggaag ggtgtggctt tcgagcggta tggctttatg gaacaggatg
      781 agtggggagt atggcggtcg ccggaagggg cgcagatagc ctggttcaag gatccagacg
      841 gcaacacgtt gtcggtggcc gagttgtgag ggcggagagc aaccgcgcag ccacatgccc
      901 gggaagtcct ccgccctgac ggacccctat tcctttacgc ccgtcgtatt cgccggccgc
      961 tcaaaatgcc actcagaccg atccagaacc cgttgcacgg gatatatttc attgcacgta
     1021 ttcccatcgt acagccgccc gttcttcatc acataagcga cggaattcgt gttcctgata
     1081 ttttccagcg ggtttttatc cattaccacc aggtctgcca gcttacccgc cttgaggcta
     1141 cccagatcac ggtccagccc caggccggta gcgcctagga tggtcgccac ccgcagcacg
     1201 tccatcggct tcatgccacc cgaagccatc gcccacatct cccaatggta gccgattcca
     1261 ttgaattcgc catgactgcc gataccagcc agcccccctg ccattacaag atcgttcatt
     1321 gtcttggcat gtttcgggaa aacctgctcg tcgtagctga accatcccgc cttgtcccgc
     1381 cgggtcttac tggcaagctc ttcgtagggc atgaggtatt gcagcttcgg atcgctgtac
     1441 gcattttcgg tctcccacca gtaattttcc gcaaacgggc cgccataggc gacaatcagg
     1501 gtgggcgtaa cgcacatttg cgagaacgcg atcgatttga caacatcctt gtacaagggg
     1561 aaaaccggga tggcgtgctc gtggccggga tatccatcga tcaggttggt caggttcagt
     1621 ttgaggttca aaccgccctc ggtcgtcggc atcagaccct ggtgacgcgc cgccatgatg
     1681 atccattcgc gcatctgccg gttgccggtg aggtacattt taatataatg cgtgttgtaa
     1741 tatttgctat actggcgaag gatattttcc gcctgggtcg agtccttgac gttataatcc
     1801 cagaaaccga cacccggccc ggtggaataa atccgcggtc caaaaacaga tccggcatcc
     1861 acctcgtcct ggtaggtcag gatatcggtc gtcgccgttt gcggatcccg cgtcgtggtc
     1921 acaccgtagg cgagattgat ggcgtatttc caaacctggt tcttgtggat tccccattgc
     1981 ggccacatat gcgaatgggt gtccacaaat ccgggaacga tggt
//
LOCUS       DATGKE010000613         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_556044, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000613 DATGKE010000000
VERSION     DATGKE010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556044"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VKU83_10385"
     CDS             complement(<1..870)
                     /locus_tag="VKU83_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI94010.1"
                     /translation="MGRRILLLYLLLFIFQGQFLVKAADPGDPPPGSLTGTITDKATG
                     NPIPGASISIPDLRTGTVTDANGKFSLSKLPSGSYLVQISFIGYATFNEKVNFSTTKE
                     LNVQLKPSTIEEAEVVVTGVSKATEIRRDPVPIVAVGKSYMQEHSAATNVIDAIANLP
                     GISAVTTGPNVSKPFIHGLGYNRVVTAEDGIRQEGQQWGDEHGIEVDQNSIDRIEIIK
                     GPASLSYGSDAIAGVINLLTPPPVPDGKTLGYVSGLYGTNNGQLNGSLRLQGNKNGFV
                     WGVQASEKAAKNYR"
     gene            complement(908..1234)
                     /locus_tag="VKU83_10390"
     CDS             complement(908..1234)
                     /locus_tag="VKU83_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94011.1"
                     /translation="MRKLNARIAALALILVFTQKLGLGIWMHNWFHETKVLKGQPLPD
                     IDKLSANCHCIDDFLVPLVETPAVIAAAPERAFQIIPCVYRTFHSTFTYSFASLRGPP
                     AVATLF"
     gene            complement(1307..>2199)
                     /locus_tag="VKU83_10395"
     CDS             complement(1307..>2199)
                     /locus_tag="VKU83_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94012.1"
                     /translation="AAAETGATIYVGAVMVDAPPAPWEPKISRDWNSGMLRILHDKAD
                     YFVVHDYFTPYNKNAGAAEILYDAATVPGRVMSHVQRSLQEAGAAVRPVAMDEWNMFA
                     AGSRQQVSNVSGLFGVLVVGEALRNKFGLAARWDLMNGWAGGNDHGLFSAGDEPGVRR
                     WAPRPSFYYLYYLQRMMGDRLIGCRIQGDTAIRAYASRFSSGEVAAALVNTSTTPVAV
                     AVDLHRIRTAKRYYWYSLQGGDDNGEFSARVIVNGTGPGGAAGGPADYATLKAFTADV
                     SGGVRVTVPARGAVFLVVGK"
BASE COUNT          489 a          704 c          550 g          456 t
ORIGIN      
        1 cctatagttc ttggccgcct tttccgaggc ctgtaccccc cacacaaaac cgttcttatt
       61 cccctgaagc cgcaacgagc cgttgagctg accgttgttc gtgccataca gcccgctcac
      121 ataccccagc gtctttccat cgggtaccgg cggcggcgtg agcagattga tgacgcccgc
      181 tatcgcatcc gaaccatagc tgagactggc cgggcccttg atgatctcga tgcggtcgat
      241 ggaattctga tcgacttcga tcccatgctc atcaccccac tgctggccct cttgccggat
      301 cccatcctcc gccgtgacca cccggttata tcccaggccg tggatgaacg gcttcgagac
      361 attcggcccc gtagtgaccg cgctgatccc cggcagattg gcgatggcat cgatgacatt
      421 cgtagccgcg gaatgctcct gcatatacga tttccctacc gctacgatcg gcaccgggtc
      481 ccgccggatc tcggtggctt tgctgacacc cgttacgact acctcggcct cttctatggt
      541 ggaaggcttc agctgtacgt tgagctcttt agtggtggag aagtttacct tttcattgaa
      601 tgtggcatac ccgataaacg agatctgtac cagatagctg ccgctcggca gtttcgaaag
      661 cgaaaattta ccgttggcgt ccgttaccgt acccgtccgc agatcgggta tcgaaatgct
      721 cgcgcccggg atcgggttcc ctgtggcttt gtccgtgatc gtccccgtta ggctgcccgg
      781 cggcggatcg cccggatccg ctgctttcac taagaattga ccctgaaaaa taaatagcaa
      841 taaataaagt agaaggattc gccgtcccat ctcaaagaat taattgagca aaaaaaagtg
      901 tctcaggtca gaagagtgta gcaacggcgg gaggacctct cagcgaggcg aaagaatagg
      961 taaaggtgga atgaaaggtt cgataaacgc aagggatgat ttggaacgca cgctcgggtg
     1021 cagctgcgat gaccgccggc gtctctacca acggcaccag gaaatcgtcg atacaatgac
     1081 agttggcgct tagcttgtcg atatccggca acggctgccc cttcaacacc ttggtctcat
     1141 ggaaccagtt gtgcatccat atgcccaatc ccagcttctg cgtgaatacc aggatcagtg
     1201 ccagcgctgc tatgcgtgcg tttaatttcc tcaacgaccc caaaagtact cctttgcaac
     1261 tgagttgcaa aaagaattat ggatgaacgg tagcgccaac ggtacctcat ttccccacaa
     1321 ccaaaaacac cgcgccgcgc gccggtacgg tcacccggac cccgccggat acatccgctg
     1381 taaaggcctt cagcgtggca taatcggccg gaccacccgc cgccccgccg ggtccggtgc
     1441 cattgacgat cacccgcgcc gaaaactcgc cgttgtcatc accgccctgc aaactatacc
     1501 agtaataccg cttggccgtc cttatcctat gcaggtcgac cgcgaccgcc accggcgtgg
     1561 tcgacgtatt gactagcgcc gccgcaacct cgcccgaaga aaaacgggag gcatacgccc
     1621 ggatcgccgt atctccctgg atacgacagc cgattagccg gtcccccatc atccgctgta
     1681 aataatagag ataataaaag gaaggccgcg gcgcccaacg ccgcaccccc ggctcgtcgc
     1741 cggcactgaa caacccatga tcattgcctc ctgcccaccc attcatcaga tcccaacgcg
     1801 cggcaagtcc gaatttgttc cgcaacgctt cacctaccac aaggacaccg aaaagtccgc
     1861 tgacattgga tacctgctgg cggctgcccg cggcaaacat attccattcg tccatcgcca
     1921 ccggcctgac cgccgccccc gcctcctgta aagatcgctg cacatggctc atgacccggc
     1981 cgggcaccgt ggcggcatca tataggatct ccgccgcccc ggcattttta ttgtagggag
     2041 taaaataatc atgcaccaca aaataatccg ccttgtcatg gaggatccgt aacatacccg
     2101 aattccagtc ccggcttatt ttcggttccc aaggggcggg cggcgcatct accatgaccg
     2161 cgcccacata gatcgtggct ccggtctcag cagccgcct
//
LOCUS       DATGKE010000614         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_979517, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000614 DATGKE010000000
VERSION     DATGKE010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979517"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VKU83_10400"
     CDS             <1..186
                     /locus_tag="VKU83_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94013.1"
                     /translation="GRNSIALSDGMATRLFGSTANVIGKSVEFQHEPHPYTVTGFFEN
                     RITILPNNSISCLPMIY"
     gene            183..1946
                     /locus_tag="VKU83_10405"
     CDS             183..1946
                     /locus_tag="VKU83_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI94014.1"
                     /translation="MKYKDPGFQDWGSTFPYTYVILRPGVSVTAFNQKLVNYLKRKTN
                     NQILYRTPFITKFSEAYLYGRYEDGKQVGGRIEYVRLFSIIAGVILLIACINFMNLST
                     AKAAGRAKEVGIKKVLGAGRFTLVLQYFGESLLMAFASLLVAILLVALVLPAFNGITG
                     KQLTLDHPGFGLILWTMVILLVTGLLAGSYPALYLSGFKPAMVLKGKLKTATSELFVR
                     KGLVVFQFALSAILIISVMVVYRQIHFIQTKKLGYDRGNVLAFYKEGKLSDPLQQESF
                     LNAVRNIAGVAQASCIGHSLAGHSGGTYGVVWAGKDPNDRTEFEIMNVDYNFQHVLNI
                     AMKEGRPYSRKYVADTNKIIFNEAAVKFMGMARPIGQKVTIWGKDFEIVGVMKDFNFQ
                     SLHEKIKPLFARLNPEDAYRFMLKIKPGKEAAAIDRLQQLYKQYNPGFPLNASFLDES
                     YQRLYAAENRVSVLSRCFAGLAILISCLGLFGLAAFNIQRRSREIGIRKVLGASVGGV
                     VLLLSRDYLKLIGFSMLIAFPLALWMTGNWLQSFVYHIRRGWSVFLLAGFLVILLTFL
                     TISFQSIKAALVNPSRSLKSE"
     gene            2157..>2664
                     /locus_tag="VKU83_10410"
     CDS             2157..>2664
                     /locus_tag="VKU83_10410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI94015.1"
                     /translation="MNLPLDFFLLIPCYNDVEGLRRSLMSVVYDRSRYGILIVDDGSD
                     TPVRIDSLGLLLPEASYVVRQNSNKGITEALNRGLGWLKKRTDYRFVARLDCGDVCSP
                     ERFIRQVDYLTRHPGVDLLGTWVRFENFETGFNYAYRTPEKREQIIKGMHFRNLFIHP
                     SVMWRATVL"
BASE COUNT          577 a          685 c          705 g          697 t
ORIGIN      
        1 gggagaaatt ccattgccct ttcggatggc atggccaccc ggctgttcgg cagcacggct
       61 aatgtgatcg gcaagtccgt tgagttccag cacgaaccgc atccctatac cgtgaccgga
      121 tttttcgaaa accggataac cattcttccg aacaattcga tttcctgctt accgatgatc
      181 tattgaagta taaggatccc ggattccagg attggggttc gacattccca tatacctatg
      241 taatcctgcg gccaggcgtt tccgtaacag cctttaatca aaagctcgta aactatctca
      301 aacggaagac caacaaccag atcctttacc gtaccccgtt cattaccaag ttctcagaag
      361 cttatctgta cggtcgctat gaagatggca aacaggtcgg cggtcgtatc gaatatgtcc
      421 gcttgttttc tattattgcc ggggtcatcc tgttgattgc ctgcatcaat tttatgaacc
      481 tttccaccgc taaagcggcc ggtagggcta aggaagttgg catcaagaag gtgctcggcg
      541 ccggtaggtt tacgctggtg ttgcaatatt tcggtgaatc gctgctcatg gcttttgctt
      601 cgttgctggt tgccattttg ctggtcgcac ttgttttgcc ggctttcaac gggatcacgg
      661 gcaaacagct gacactggat catccgggtt ttggtttgat cttgtggaca atggtgatcc
      721 tgcttgttac ggggttactc gccgggagtt atcctgccct gtatctgtcc gggttcaagc
      781 ccgccatggt cctgaagggc aagctcaaga ccgctaccag cgaactcttt gtgcgtaagg
      841 gtttggtggt atttcaattc gccctttcgg cgatcctcat tatctccgtg atggtggtgt
      901 accggcagat ccattttatc caaaccaaga agctgggtta tgaccgtggc aatgtcctgg
      961 ctttttacaa ggaaggaaaa ttatcggacc ctctgcaaca ggaaagcttt ttgaatgctg
     1021 tccggaatat cgcgggtgtc gcgcaggctt cctgcatcgg tcattccctg gccgggcata
     1081 gcggcggaac atatggcgtg gtgtgggccg ggaaagatcc caacgacagg acagaattcg
     1141 aaatcatgaa cgtcgattac aatttccagc atgtgctcaa tatcgccatg aaggaaggac
     1201 gcccctattc ccgcaagtat gtggcggaca ccaataagat catattcaat gaagccgccg
     1261 tcaaattcat gggtatggct cgtcctatcg gtcaaaaagt taccatctgg ggcaaggact
     1321 tcgagatcgt cggtgtcatg aaggatttca attttcaatc cttgcatgag aagatcaaac
     1381 cacttttcgc caggctgaac ccggaggatg cctaccgctt tatgctgaag atcaaacccg
     1441 gcaaggaggc cgctgccata gaccggttac agcaactgta caagcagtac aatcccggct
     1501 tcccgctcaa tgccagtttc ctcgatgaga gctatcagcg cttatacgct gccgaaaacc
     1561 gggtatccgt cctttcccgt tgttttgctg gtcttgccat cctgatctct tgtctgggac
     1621 tgttcggtct ggctgccttt aacatccagc gccgtagcag ggagatcggt atccgcaaag
     1681 tgctcggtgc atcggtaggc ggagtggtcc tgctgctttc cagggactat ctgaaactga
     1741 tcggtttctc catgttgatc gccttcccgc tggccttgtg gatgaccggg aattggctac
     1801 aatcttttgt ttatcatatc cggcgcggtt ggagcgtatt cctactggcc gggttcctgg
     1861 tgatcctgct gacttttctt acgatcagtt tccaatcgat caaggctgca ttggtcaatc
     1921 cttccaggag tctgaaatcg gaatgatctg atcttatatt taaaattgat cctccgaaga
     1981 ctccccagcg ggaagcgtcc gccagtgggc gaactcgtcc aaacgacgat gatccgccgg
     2041 catttccgtc tgggactccg cgctttacga ctgcggcgta gaacacccgc gatggcccgg
     2101 agtccggcag ccgaccgggc ggcccggcat ggcaaataaa ttgcaagctg ttccgtatga
     2161 acctgccgct ggattttttt ctattgatcc cctgttataa cgatgtggaa ggtctgcgcc
     2221 ggtcgctgat gagcgttgtc tacgaccgca gccggtatgg tattctcatc gttgatgacg
     2281 gcagcgatac tccggttagg atcgattcgc ttggcctctt attgcccgag gcgagttatg
     2341 tcgtccggca aaacagcaac aaaggtatta ccgaagcgct gaaccgcgga ttgggctggc
     2401 tgaaaaaaag aaccgactat cggttcgttg cccggctcga ttgcggtgac gtttgcagcc
     2461 cggaacgttt catccggcag gtggattatc tgaccaggca tcccggcgtc gatctgctgg
     2521 gcacatgggt gcgttttgag aatttcgaaa ccggattcaa ctacgcgtac cggacgcctg
     2581 aaaaacggga acagatcatc aaggggatgc attttcgcaa tttgttcatc catccgtcgg
     2641 tgatgtggcg ggccacggta ctgg
//
LOCUS       DATGKE010000615         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_299127, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000615 DATGKE010000000
VERSION     DATGKE010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299127"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /locus_tag="VKU83_10415"
     CDS             <1..2077
                     /locus_tag="VKU83_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLI94016.1"
                     /translation="GLAGHTLGHYLSACSMEYAATGDARFRVRVNYIVTELALCQKAR
                     GSGYIGAIPREDSLWSEVAAGKIRSHGFDLNGAWSPWYTVHKVMAGLLDAYLYAGNAE
                     ALRVERAMADWTGTIVAHLSDSVRQRMLACEYGGMNEVLANTYAITGDRKYLALSYDF
                     YDRRILDSLAEGLDDLPGKHSNTQIPKVIGSARRYELTADQRDKQIAESFWSIVVSEH
                     SYATGGNSDYEYFGQPGKLNDELTDNTTETCTSYNMLKLTRHLFAWKPSAALMDYYER
                     TLYNHILASQNHQDGMTCYFVPLRMGGRKEYSDSFNTFTCCVGSGMENHVKYGQEIYS
                     QGSDGSLFVNLFISSRLDWKEKGIDVEQHSGIPAADRVLLTITKAPRASRWTLRIRKP
                     YWAGRNIRLLVNGQLYRKWVAGYDGYIAVRRTWKKGDRIVIELPEDFHSEGMPDNPSR
                     VALFYGPVLLAGELGIKEPDPVTGIPVLVSTSADPNRWMRRMPGDSLVFRTVSAGQPA
                     DITLIPFNRTADEYYSVYWDLFTPQGWALRQEKYEEDRARAQQLEDRTIDRLRLGEMQ
                     PERDHSFAGEGLQGGDAHGRHWRSAEDGGYFSFTMKVDGNGGNTLICDYWGDDHRGRI
                     WDIQVDGQTIATQNLASFRESKFYEISYPVPPELVKGKKAVTVKFVARSARNGVGPVS
                     GVIRMVRSS"
     gene            complement(2156..>2372)
                     /locus_tag="VKU83_10420"
     CDS             complement(2156..>2372)
                     /locus_tag="VKU83_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI94017.1"
                     /translation="TLHTFVGFLNEVRINEACKKLADGGYDSIAAVAYHCGFNSITNF
                     NRVFKTVVGKSPSQYVGTYRQHVAAAE"
BASE COUNT          496 a          648 c          739 g          489 t
ORIGIN      
        1 ggggctggcc gggcatacat taggccatta cctgtccgcc tgctcgatgg aatatgcggc
       61 aacgggcgac gcccgtttcc gcgtccgcgt caattatatt gtcaccgaac tggcgctttg
      121 tcaaaaggcg agaggcagcg gatatatcgg cgccattccg cgggaggaca gtctctggtc
      181 ggaggtggcc gcggggaaga tccgctctca cggttttgat ctgaacggcg catggtcgcc
      241 ctggtatacc gtccacaagg tgatggccgg cctgctggac gcctatctct atgcggggaa
      301 cgcggaagca ttacgggtgg aacgcgcgat ggctgactgg accggcacga tcgtcgcgca
      361 tttgagcgac tcggtgcgcc aacggatgct ggcctgcgag tacggcggca tgaatgaagt
      421 gctggccaac acttatgcta tcacgggaga ccggaaatat cttgcgcttt cgtatgattt
      481 ttacgaccgg cggatactcg actcgctggc ggaggggctg gacgatctgc ccggcaagca
      541 ctccaatacg cagatcccga aggtgatcgg ttcggcgcgg cgctacgagc ttacggcgga
      601 tcagcgggac aagcagattg cggaatcgtt ttggtccatc gtggtcagcg agcattcgta
      661 cgccaccggc ggtaacagtg attatgaata ttttggccag ccggggaagc tgaatgatga
      721 actgaccgac aatacgaccg agacctgcac cagttacaat atgctgaagc tgacgcggca
      781 tcttttcgcc tggaaacctt ccgccgccct gatggactac tatgagcgca cgttatacaa
      841 tcatatcctt gcctcccaga atcaccagga cggaatgacc tgttatttcg tgccgttgcg
      901 catgggcggc cgcaaggaat acagtgattc ctttaatacc ttcacctgtt gcgtcggatc
      961 ggggatggag aaccatgtca aatacgggca ggaaatctac tcgcagggtt ccgacggcag
     1021 cctcttcgtc aaccttttca tttcatcgcg gctggactgg aaggaaaaag gcatcgacgt
     1081 cgaacagcac tcgggcatcc ccgccgctga ccgcgtgttg ctaaccatta caaaggcccc
     1141 gcgggcttcg cgttggacac tccgcatccg caaaccgtat tgggcaggcc ggaatatccg
     1201 cctgctggtc aacggccagc tgtacaggaa atgggtggcc ggctatgatg gctatatcgc
     1261 ggtcaggaga acctggaaga agggagaccg catagtcatc gagctgccgg aagatttcca
     1321 ttcggaggga atgcccgata atccttcccg cgttgcgcta ttctacggac cggtgttgct
     1381 cgccggcgag ctgggcatta aggagcccga cccggtgacc ggtatcccgg tgctggtgtc
     1441 caccagcgct gaccccaacc ggtggatgag gaggatgccg ggggattcgc ttgtgttccg
     1501 gacggtatcg gccggtcagc cggccgatat cacgttgatt ccatttaacc ggacagccga
     1561 cgaatattac agcgtgtatt gggacttgtt cacgcctcag ggctgggccc tgcggcagga
     1621 gaaatatgag gaggatcgcg cgcgggctca gcagttggaa gaccggacga tcgaccggtt
     1681 gcggcttggc gaaatgcagc ccgagcggga ccattctttt gccggggaag gactgcaggg
     1741 cggcgatgcg catggccgtc attggcgttc agcagaggac ggcggctatt tttcatttac
     1801 aatgaaggtc gacgggaatg gaggcaatac gctgatctgc gactattggg gggatgacca
     1861 ccgcgggcgg atatgggata tccaggtcga tgggcaaacg atcgcgactc aaaaccttgc
     1921 ttcgttcagg gaaagcaaat tctatgagat ctcctatccg gtgccaccgg aactcgtaaa
     1981 gggcaagaaa gcggtcacgg tgaagtttgt ggcccgatcc gcccgaaacg gcgtcgggcc
     2041 ggtatcgggc gtcatcagga tggtccgatc atcataggcc ggcgaccacc ccgccggctt
     2101 cgccctcgca gcccgcgcgg gtctcgccac acccgggccc ggagactctc gaaggttatt
     2161 cagcagctgc gacatgctgc cggtacgttc cgacatattg cgagggtgat ttccccacaa
     2221 ccgttttgaa gacccggtta aaattggtga tgctgttaaa tccgcagtgg tatgcgacgg
     2281 cagcgatgct gtcatagccg ccgtctgcca gtttcttgca ggcttcgttg atccgcactt
     2341 cattcaggaa cccgacaaag gtgtgcaggg tg
//
LOCUS       DATGKE010000616         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1074321, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000616 DATGKE010000000
VERSION     DATGKE010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074321"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VKU83_10425"
     CDS             <1..1035
                     /locus_tag="VKU83_10425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLI94018.1"
                     /translation="GGGGSEVAIQFVSDKENYPAALDLLADILLHPSFDKNEFDKLLI
                     DQNSGLEQYRTDPQQVAFNTMNRTMEPYPQGHPKYTESFDELSAELKAAKLDDVRQFY
                     QDFYGADHGYAAFVGGIEPAVVQGILEKAFAGFKSKQPYAEIEEKYFDVKGGSKDIQI
                     PDKKNAVFVGGLNLPLKQSDMDYMAMDMANEMLGGGTFIANRISNRLRETEGMSYGAG
                     SYMTFNYKYASCTWGAYAIFNPIYKTRLDSAFRDVLVKTLKDGFQQEEFRKAVAAWKQ
                     QRKIGLGVDQSLASRLTAYMKDGKDLSFYTYNEEKVSALTLEQVNAALRKYIDPEKIS
                     YVFVGDFNKK"
     gene            1105..1482
                     /locus_tag="VKU83_10430"
     CDS             1105..1482
                     /locus_tag="VKU83_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94019.1"
                     /translation="MGVMSHRGPGSVEVRNGTWPIRLERSTDRPGVSFSLIFRDQEVM
                     NDQVLDTLDFADAEQLHYLGAALTALKNGHNGDAAKSKDYSITRADKKFAGGVWYILR
                     PQYGSTSFQQPEADIIVKAIKQW"
     gene            1524..1970
                     /locus_tag="VKU83_10435"
     CDS             1524..1970
                     /locus_tag="VKU83_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94020.1"
                     /translation="MKKLIILFFFLPILAKAQFVIVNNSTTSLIEPRTGTWPLDLQRV
                     IRESDTSYVLAFRDQQEASVVNMSTLAFGNVEQLRYFMKGLTALRTGSTGETAKFKEY
                     TIKRMDVRKPGEKGGTVWYILQCNDGTTTKFLQPEADSMIAAIKGL"
     gene            complement(1965..>2618)
                     /locus_tag="VKU83_10440"
     CDS             complement(1965..>2618)
                     /locus_tag="VKU83_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLI94021.1"
                     /translation="LIELCSPAGYKTIGFGFLAGMPTADIRRTIESAGLRCPSCHFGF
                     PELTDHIDESIAFAQAMGLSQMICSTFWLPKTAHLSDYQAAADKLNKAAEKIKAAGMQ
                     TGFHNHEFEFQTLDGSLIYDALLARFDPGLVKMQFQTEVINLGYKASTYFEKYPGRFI
                     SSHLSDWTTDKKEVPVGQGVINWKEFFNAAKTGGVKNYFVEMNFDTFSDSAKYIKGL"
BASE COUNT          640 a          543 c          822 g          613 t
ORIGIN      
        1 gggggcggcg gttccgaggt agcgatccag tttgtgagtg acaaggagaa ttaccccgcg
       61 gcgctggatc tgttagccga tatcctactg catccttctt tcgacaagaa tgaattcgac
      121 aagctgttga tcgatcagaa cagcgggttg gagcaatacc ggaccgatcc gcagcaggta
      181 gcattcaata cgatgaacag gacgatggag ccgtatccgc aggggcatcc gaagtatacg
      241 gaaagtttcg acgagctatc ggctgagctg aaggccgcca agttagacga cgtgcggcag
      301 ttctatcagg atttttatgg agcggatcat gggtatgcgg cgttcgtagg gggtatcgaa
      361 ccggcggtgg tgcagggtat cctggaaaag gcgtttgccg gattcaagag caagcagcct
      421 tatgcggaga tcgaagaaaa atatttcgat gtgaagggtg ggtcgaagga tatccagata
      481 ccagacaaga agaatgccgt attcgtgggt ggtctgaatc tgccgctgaa gcagagcgat
      541 atggattaca tggcaatgga catggccaat gagatgcttg gcggcggaac gttcatagcg
      601 aaccgcatct cgaaccggct gagggaaacg gaggggatga gctatggcgc cggcagctat
      661 atgacgttca actataagta cgcgagctgt acctggggag cgtatgcgat cttcaatccc
      721 atctataaga ccaggctgga cagtgcgttc cgggatgtgc tggtgaagac gttgaaagac
      781 ggttttcagc aagaggagtt ccggaaggcg gtagcggcgt ggaagcagca acggaagatc
      841 ggattgggcg tagatcagag tctggcgagc cggttgacgg cgtatatgaa ggatggcaag
      901 gatctaagtt tttatacgta caatgaagag aaagtaagtg cgttaacatt ggagcaagtc
      961 aatgcggcgt tgcggaaata tattgatcct gagaagataa gttatgtgtt tgtgggggat
     1021 ttcaataaga agtgagtttg gcgagattgt tgcagtaatt catgctgata ttttatgaaa
     1081 aagctattgt taactgttct tagtgtggga gttatgtctc accgcgggcc cggctcagtg
     1141 gaagtccgga atgggacctg gcctatccgt ctggagcggt cgacggacag accgggtgta
     1201 agcttttcct tgatcttcag ggatcaggag gtgatgaatg atcaggtgct ggacacgctg
     1261 gattttgcgg atgcggagca gctgcattat ttaggagcgg ctttgacggc attaaagaac
     1321 gggcataatg gcgatgctgc gaaatccaag gattattcga tcacgcgggc cgataagaaa
     1381 ttcgccggag gggtatggta tatacttcgg ccgcagtatg ggtcgacgag cttccagcaa
     1441 ccagaagcgg atatcattgt gaaggcgatc aaacagtggt gaggagacat actaaatttt
     1501 tcaaccaatc gttaatccat actatgaaga agctaatcat cctattcttt ttcctaccta
     1561 ttcttgcgaa ggcgcaattc gtaatcgtga ataattcgac gacgagcctg atcgagccgc
     1621 gtaccggcac ctggccgttg gatctgcagc gggtcatccg ggaatcggat acctcctatg
     1681 tgctggcgtt ccgggatcag caggaggcct ccgttgtgaa tatgagcact ttggcttttg
     1741 gcaatgtgga gcagctgcgg tatttcatga aggggttgac ggcattgcgg acggggtcca
     1801 cgggggaaac ggccaaattc aaggaatata ccattaagcg gatggatgtc aggaagccgg
     1861 gggaaaaagg tggtacggta tggtacatcc tgcaatgcaa tgatggcacc accaccaaat
     1921 ttctgcagcc ggaggcggat agcatgatcg cggcgatcaa ggggttatag acccttaatg
     1981 tatttggcgc tgtcggaaaa ggtatcgaaa ttcatttcca caaaataatt tttcacgccg
     2041 ccggtttttg cggcgttgaa aaactctttc cagttaatca caccctggcc gacgggcact
     2101 tcttttttgt ccgtggtcca gtccgagaga tgggatgaga taaagcgtcc ggggtatttc
     2161 tcgaagtagg tggaggcttt atatcccagg ttgatcactt ccgtctggaa ttgcattttc
     2221 accagacctg ggtcgaaccg ggccaggagg gcatcgtaga tgagcgatcc gtcaagggtc
     2281 tggaattcga actcgtggtt atggaagccg gtctgcatac cggcggcctt gatcttttca
     2341 gcggctttat tgagcttatc tgcagcggcc tggtagtcgc tgaggtgagc ggtcttgggc
     2401 agccagaagg tgctgcagat catctgagag aggcccattg cctgtgcaaa ggcgatgctc
     2461 tcgtcgatat ggtctgtgag ttccgggaat ccgaaatggc agctagggca gcggaggcct
     2521 gccgactcga tggtgcggcg gatatcggcg gtcggcatgc cggccaggaa accgaagccg
     2581 atggttttat agccagcggg ggagcagagc tcgatgag
//
LOCUS       DATGKE010000617         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1134071, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000617 DATGKE010000000
VERSION     DATGKE010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134071"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VKU83_10445"
     CDS             complement(<1..229)
                     /locus_tag="VKU83_10445"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HLI94022.1"
                     /translation="MHSHLIPAIDDGAPDLPTSLQLIRGMIDLGYKKLITTPHVNGDI
                     FPNTPEVIASGLEAVRAELRRESVDMPIRAAA"
     gene            complement(302..1228)
                     /locus_tag="VKU83_10450"
     CDS             complement(302..1228)
                     /locus_tag="VKU83_10450"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HLI94023.1"
                     /translation="MNTSDKIYIAGHRGMVGSAIWRRLERAGYTNLLGRPSAQLDLKN
                     QAAVQAFFDEERPDYVFLAAAKVGGIQANDIYRGEFLYDNLMIQNNTIDAAYRTGVRK
                     LMFLGSSCIYPKLAPQPLKEEYLLTGLLEPTNEPYAIAKIAGIKLCDAYRAQYGCNFI
                     SVMPTNLYGPNDNYDLNNSHVLPALIRKFHEAKMKAAPSVTIWGSGKPRREFLHADDL
                     ADACFYLMQHYDEPGLVNIGTGEDLEIGELARLIMRIVGYTGDIVHDTSKPDGTPRKL
                     MDVSKLRSFGWKPKIGLEEGISAVYAEYKKQH"
     gene            complement(1268..2383)
                     /gene="gmd"
                     /locus_tag="VKU83_10455"
     CDS             complement(1268..2383)
                     /gene="gmd"
                     /locus_tag="VKU83_10455"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLI94024.1"
                     /translation="MAKVALITGVTGQDGAYLSELLLNKGYIVHGIKRRSSLFNTDRI
                     DHLYQDPHESNVHFSLHFGDLTDSTNLIRIIQETQPDEIYNLAAMSHVQVSFETPEYT
                     ANADGIGTLRILEAVRLLGLAEKTRVYQASTSELYGLVQAVPQTEKTPFYPRSPYAVA
                     KLYAYWITVNYREAYKMYATNGILFNHESPLRGETFVTRKITRGVAKIGLGLQDKIFL
                     GNLDARRDWGHAKDYVEAMWRILQQPEPEDYVIATGVTTPVRDFVQMAFAEIGIQLEF
                     RGKGDKEVAVVSASANPDFMVPVGKEVVAVDARYYRPTEVDLLIGDPSKAQRKLGWKP
                     QYDLPMLVKEMVGSDVQLFRRERILKDSGFHIKNQYE"
     gene            complement(2392..>3013)
                     /locus_tag="VKU83_10460"
     CDS             complement(2392..>3013)
                     /locus_tag="VKU83_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI94025.1"
                     /translation="GTLAVVAGFPHVAKVLSEKDKGVYDAMNKGIGLATGEVIGILNS
                     DDIYVDPSVLSGVARAFEDPAVMTVYADLQYVHPDNTGRVKRTWITGPFRKKSFYFGW
                     MPPHPTFFVRKVVYDQIGRFNTDLRSAADYELMLRILVRHRMSTHYIPRVVVKMRAGG
                     LSNASLWNRLRANREDRLAWKLNGLRPYFFTLYLKPLRKITQFINT"
BASE COUNT          644 a          848 c          817 g          704 t
ORIGIN      
        1 cggccgcagc ccgaatgggc atgtcgacag actcccgcct caactcagcc cggaccgcct
       61 cgagcccgct cgcgatcact tcgggggtat tcgggaaaat atctccgttg acatgagggg
      121 ttgtaatgag tttcttatat cccaggtcga tcatgccgcg gatgagctgc agcgaggttg
      181 gaagatccgg cgcgccgtca tcgatggcgg ggatcagatg actgtgcata tcacaggaaa
      241 ggcgctccaa tgcaccggac ttcgggtaat tccgcctaaa gattgaaaac accgcctgaa
      301 attaatgctg ttttttgtat tccgcgtaaa ccgcggaaat tccttcttcc aagccgatct
      361 ttggtttcca gccaaagcta cgcagcttcg agacgtccat tagttttctc ggcgtgccgt
      421 cgggtttgct ggtgtcgtga acgatgtcac cggtatagcc gacaatacgc atgatcagcc
      481 tcgccagctc gccgatctcc aggtcttcgc ccgtcccgat gttgaccaac ccgggttcat
      541 cgtagtgctg catcaggtaa aagcaggcat ccgcgaggtc gtcggcatgc aggaactccc
      601 tgcggggttt acccgacccc caaatggtca ccgaaggcgc cgccttcatt ttggcctcat
      661 gaaatttccg gatcagggcc ggcagcacat gggaattgtt aagatcgtaa ttgtcatttg
      721 gcccgtacag gttcgtcggc atcacggaga tgaagttgca gccgtattgg gcgcgatagg
      781 catcgcataa ttttatgccg gcaatcttgg cgatggcgta gggctcattc gtcggttcca
      841 gtaacccggt cagtaaatac tcttctttca gcggctgggg ggccagcttg ggatagatgc
      901 aggaagaccc caggaacatc agcttcctga cgccggttcg gtaggccgca tcgatcgtat
      961 tgttttggat catcaggttg tcataaagga attcgccccg atagatgtca ttcgcctgga
     1021 tgccgcccac cttggccgcc gccaaaaaaa cgtaatccgg ccgctcctcg tcaaaaaagg
     1081 cctggaccgc cgcctggttc ttcagatcca actgcgccga aggcctgccc aggaggttgg
     1141 tatagccagc ccgttccagc cggcgccaga ttgccgagcc gaccataccg cgatggccgg
     1201 cgatgtaaat cttatccgat gtgttcattt gcataatcct ttaagccaac cctgttgtaa
     1261 gcttacctta ttcatactga tttttgatat gaaagccgga atccttcagg atccgttccc
     1321 ttctgaagag ctgaacgtcg gacccgacca tctcctttac gagcatgggc aggtcgtact
     1381 gcggtttcca gccgagtttc cgctgcgctt ttgatggatc gccgatcagc aggtccactt
     1441 cggtgggccg gtaatacctg gcgtccacgg ccacgacctc tttaccgacg gggaccataa
     1501 agtccggatt ggcgctggcg ctgactaccg cgacttcctt atcgccctta cccctgaact
     1561 cgagctggat gccgatttcg gcaaaagcca tctggacgaa gtcccgcacg ggggtggtca
     1621 caccggtggc gatgacgtaa tcttcgggtt cgggctgttg caggatgcgc cacatggcct
     1681 ctacgtaatc tttggcatga ccccaatcgc ggcgggcgtc caggttgcca aggaatatct
     1741 tgtcctgcaa gccgaggccg atcttggcca ctccgcgggt gatcttccgg gtaacaaagg
     1801 tctcgccccg caacggtgat tcgtgattga acaggatccc gttcgtcgca tacatcttat
     1861 aagcctcgcg gtagttgacc gtgatccaat aggcgtacaa cttggctacc gcatagggcg
     1921 aacggggata gaagggcgtc ttttcggtct gcgggaccgc ctggaccaat ccgtacagct
     1981 cggaggtgga cgcctgataa accctggtct tttctgccag gccgagcaac cttacggctt
     2041 ccaggatgcg aagcgtgccg atcccgtccg cattggcggt atattcgggc gtttcgaagc
     2101 tgacctgaac atggctcatc gcggcgagat tatagatctc gtcgggctgg gtctcctgga
     2161 tgatccggat caggttcgtg gagtcggtca ggtcaccgaa atgcaacgaa aaatggacat
     2221 tcgattcatg cggatcctgg tagaggtgat cgatgcggtc ggtattgaac aacgagctcc
     2281 gtcttttgat cccatggact atgtatcctt tattcagcaa caattcactt aaataagctc
     2341 cgtcttgtcc ggtaacccct gtaatgagcg cgactttagc catggcctgt tttatgtatt
     2401 tataaattgg gtgatctttc gcaaaggttt aagatatagc gtaaagaaat aaggtctcaa
     2461 tccattcaac ttccatgcca gccggtcttc gcggtttgcc cgtaaccggt tccacagcga
     2521 ggcattgctc agcccgccgg cccgcatttt cacgacgacc cgcggaatgt agtgcgtcga
     2581 catgcgatgc ctgaccagga tccggagcat cagctcatag tctgccgcag accgcaggtc
     2641 cgtattgaag cgcccgatct gatcataaac cacttttcgg acaaaaaagg tcgggtgtgg
     2701 cggcatccag ccgaaataga agcttttctt ccggaaggga ccggtgatcc aggtccgctt
     2761 caccctgccg gtattatccg gatggacgta ttgcagatcg gcatacaccg tcatcacggc
     2821 tggatcctca aaagcccggg caacacccga gaggacggac ggatcgacat agatatcatc
     2881 ggaattgaga atgccgatca cctcgccggt tgccagaccg atccccttgt tcatcgcatc
     2941 ataaacgcct ttgtcctttt ccgaaaggac ctttgccacg tgcggaaacc ctgctacgac
     3001 agcaagcgtg ccg
//
LOCUS       DATGKE010000618         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_49497, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000618 DATGKE010000000
VERSION     DATGKE010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49497"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VKU83_10465"
     CDS             <1..416
                     /locus_tag="VKU83_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLI94026.1"
                     /translation="QGAQEEGLIRHIGLSEVSIEDIKKAETYVEIVSVQNKYSHDFRK
                     WEPVLEYCRETNKAFIPWYPLNAGDINSMKKIGKVAGQLGATPYQVALSWLLHHSPNI
                     LLIPGTGNVKHLEENMKAEQLHLTEEMLDELESGQ"
     gene            complement(442..933)
                     /locus_tag="VKU83_10470"
     CDS             complement(442..933)
                     /locus_tag="VKU83_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLI94027.1"
                     /translation="MTSELYELTANTPQGNPVSLSEYKGKVILVVNTATQCGLTPQFE
                     GLERLYQQYRNKGFMVLGFPCNQFAGQEPVANENMEQTCRVNHGVTFPLFEKVDVNGP
                     NTHPVFRLLKRRLGGFLGSRIKWNFTKFLVDAAGRPVKRFSPVTKPDAIEPYIRRMLD
                     SRA"
     gene            985..2121
                     /locus_tag="VKU83_10475"
     CDS             985..2121
                     /locus_tag="VKU83_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI94028.1"
                     /translation="MLRFITIVILLLVSLLAAFPAPAFPLWLLAMIVQGYPWIFVLLM
                     LVLLLAGGYKGRFGLYNALACAFALVLFVSPVVRAYSVARSLGRRLELAFTPPDSPRV
                     ARDSSSAFSLGGIFRFGTDQVAYRTLSYDTAEGKPLTLDLYPAQHLGLRPCVVVIHGG
                     AWRSGDSRQLPALNSVLARDGYVVAAINYRLAPRYKSPLQIADVAAAFRYLRSNCARF
                     SIDTSSFVLLGRSAGAQIALEAAYTLNESGLRGVIDLYGPADMVWGYAHPANKWVLDS
                     RKVMADYLGGSYEAVPQQYLRSSPIQAVTTQSVPTLMIHGLEDPLVAYEQSRRLNAKL
                     ADSNVAHYLIKLPWATHGFDFNLYGPGGQLSTYAIQRFLREVAP"
     gene            2118..3083
                     /locus_tag="VKU83_10480"
     CDS             2118..3083
                     /locus_tag="VKU83_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLI94029.1"
                     /translation="MKAIWITRKGGPEVLELREVDTPAPAAGEVLIRVAAAGLNRADI
                     YTRSSASYGGGTRIPGLEVSGIIESTGPGVSRWKAGDAVCALLNGGGYAEYAAVPAGQ
                     CLPIPAGWTVEEAAALPEAVLTVWLNVVQTARLRSSENFLVHGGTSGIGITAIRLALA
                     LGATVYTTAGTDEKCRWCERWGAKKAVNYKTADFEMELAGTAIDVILDMTGGDFTIKN
                     LRLMREDGRLVFINAMRGKDSLIDIREIMAKRIIITGSMLRPRDAAFKAALTADVEAR
                     AWPLISGGALTPVIYRVLPLAEAAEAQRIMERSEHFGKILLRVGS"
     gene            complement(3117..>3362)
                     /locus_tag="VKU83_10485"
     CDS             complement(3117..>3362)
                     /locus_tag="VKU83_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94030.1"
                     /translation="EGLQLEREFYSPRYATEEEINSHLPDFRNVLYWEPMLQTSALGS
                     GGLSFYSSDLPGKYVVVVEGITADGRAGSGIGSFDVR"
BASE COUNT          684 a          981 c         1018 g          679 t
ORIGIN      
        1 tccagggcgc gcaggaagaa gggctgatcc ggcacatcgg gctgtccgag gtcagcattg
       61 aggacatcaa aaaggcagag acctatgtcg aaatcgtctc cgtgcaaaat aagtacagcc
      121 atgattttcg caaatgggag ccggtgctcg aatattgccg cgaaaccaat aaagcattta
      181 ttccctggta tccgctgaat gccggagaca tcaattcaat gaaaaagatc ggaaaggtcg
      241 cggggcagct cggcgctacg ccctaccagg tggcgctgtc ctggctactg caccattcgc
      301 cgaatattct gctgattccc ggaacgggca acgtgaagca tcttgaggag aatatgaagg
      361 ccgaacaact gcatctcacc gaagagatgc tcgacgagct ggaaagcggc caataaggat
      421 cgggtgtcgc cggcgagcca ttcaggcacg ggaatcgagc atccgtcgga tatagggttc
      481 gatagcatcc ggcttcgtca cgggcgagaa tcgtttcacg ggcctgcccg cagcatcgac
      541 gaggaatttg gtaaaattcc atttgatgcg gctgcccagg aagccgccga gcctccgctt
      601 gagcaaccgg aacaccggat gggtatttgg cccgttgacg tccacctttt cgaataatgg
      661 gaaagtaacg ccatggttca ccctgcacgt ctgctccata ttctcattgg caaccggttc
      721 ctggccggcg aactggttgc acggaaaccc cagcaccatg aatcccttat tccggtactg
      781 ctgataaagg cgctccagtc cctcgaactg cggcgtcagc ccgcattggg tagcggtgtt
      841 cactaccagg ataaccttac ctttatattc ggataggctg acagggtttc cctggggagt
      901 attagcggtc agctcgtaga gttcgcttgt catggataaa agaatggaga tctaaagata
      961 cgcctaatat acaatgcctt cgccatgctg aggttcataa cgattgtcat cctgctgctg
     1021 gtctcgctac tcgctgcatt tcccgcgccg gcctttcccc tgtggcttct cgccatgatc
     1081 gtgcagggtt atccctggat ctttgttctg ttgatgctcg tgctcctgtt ggcgggaggc
     1141 tacaagggca ggtttggcct gtacaatgcc ctggcctgcg ccttcgcgct ggtactcttt
     1201 gtatcacccg tcgtccgggc ctattccgtc gccaggagcc ttggccgcag gctggagctg
     1261 gcatttaccc cgcccgacag ccccagggtg gcacgcgaca gcagcagtgc gttcagcctc
     1321 gggggaattt ttcgctttgg caccgaccag gtcgcttatc ggacgctatc ctatgatacg
     1381 gctgagggga agccgctgac gctcgacctt tacccggcgc agcatttggg gcttcgaccg
     1441 tgcgtggtcg tgattcacgg cggtgcgtgg agaagcggcg acagccgcca attgcccgcg
     1501 ctgaacagcg tcctggcgcg ggacggctac gtcgttgcgg caatcaatta ccggctcgcc
     1561 ccgcgctaca aaagcccgct gcaaattgcc gacgtcgcgg cggcgttccg atacctgcgc
     1621 agcaactgtg cccggttctc tatcgatacc agctcctttg tgctgctggg ccgttcggcg
     1681 ggagcccaga tcgcgctgga ggcggcctac accttgaacg aatccgggct caggggagtg
     1741 atcgatttat atgggccggc ggacatggtc tggggctacg cgcatccggc aaataaatgg
     1801 gtactcgatt cccggaaggt catggctgat tatctcggcg gctcgtatga agccgtgccg
     1861 cagcaatacc tgcgcagttc gccgatccag gcagtgacga cacaaagcgt tcccaccctg
     1921 atgatccatg gcctggagga cccactggtg gcgtatgaac aaagccggcg attgaacgcg
     1981 aagctggcgg acagcaatgt cgcgcattac ctgatcaaat tgccctgggc gacccatggt
     2041 ttcgacttca acctgtatgg ccccgggggt cagctgtcga cttacgccat tcaacgattc
     2101 ctgcgggagg ttgcaccatg aaagcaatct ggataacacg aaagggcgga ccggaggtcc
     2161 tggaactccg ggaagtggat acgcccgcgc cggccgccgg tgaagtattg atccgcgtgg
     2221 ccgcggccgg actaaaccgg gccgatatat acacccgctc ttccgcctcc tatggcggcg
     2281 ggacgcgaat cccgggcctg gaagtgtccg gcatcatcga atccacgggc ccgggcgtga
     2341 gccggtggaa ggcgggcgac gccgtctgcg cgcttcttaa cggcgggggg tatgcggagt
     2401 atgcagccgt tccggccggg caatgccttc cgatccctgc cggatggacc gtcgaggagg
     2461 ctgccgcgct gcccgaagca gtgctgacgg tctggctgaa cgtcgtgcag acagccaggc
     2521 ttcggagcag tgagaatttc ctggtgcacg gcgggacaag cggcatcggc atcaccgcca
     2581 tccggcttgc gctggcgctg ggcgcaacag tctataccac cgcgggcacc gatgaaaaat
     2641 gtcgttggtg cgagcgctgg ggcgcgaaga aggccgtgaa ttacaagacg gcggattttg
     2701 aaatggaact ggccggaaca gccatcgacg tcatcctcga tatgacagga ggcgacttca
     2761 cgatcaaaaa cctccgcctc atgcgcgaag acggccgcct ggtgtttatc aatgccatgc
     2821 gcggcaagga ttccctgatc gatatccggg agatcatggc caagcgcatc atcatcaccg
     2881 gcagcatgct tcggccccgc gatgccgctt tcaaggcggc gctgacggcg gacgtggagg
     2941 cccgtgcgtg gccactgatc tcgggaggcg cactgacgcc ggtgatctac cgggtgttac
     3001 cgctcgccga agctgctgaa gcgcagcgta tcatggaacg cagcgaacat tttgggaaga
     3061 tactcctgcg ggtcggatcc taaaagggct tcacgggccg tcgctgccgc tccgggctag
     3121 cggacgtcga aactgccgat cccgcttccg gcccggccgt ccgcggtgat cccttcgacc
     3181 acaacgacat attttcccgg caggtcggat gaatagaagc taagtccgcc tgaccccagg
     3241 gcgctggtct gcagcatcgg ttcccagtac aatacattcc ggaaatcagg aagatggctg
     3301 ttgatttcct cttcggtcgc atagcgaggg gaataaaact cccgctccag ctgcaatcct
     3361 tc
//
LOCUS       DATGKE010000619         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_879562, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000619 DATGKE010000000
VERSION     DATGKE010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879562"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VKU83_10490"
     CDS             complement(<1..1382)
                     /locus_tag="VKU83_10490"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI94031.1"
                     /translation="MKIAACLLIALSAVPAEVYCQSGGNGRFWLVVINEQHLGINGAT
                     VKLIRNGKPAGTAVAGQDGRVLFGSLPKGVYTFLISSAGYQPKMTPEYRLPGPPGDSI
                     VLAPRAATLQEATVTARALPVERKRDRTIINVDASPVNTGATVLEVLERSPGVTVDRN
                     GGISLNGKPGVLVMIDDKPTYLTGDDLNNLLSSMNAAQVAKIELISNPPARYDAAGNA
                     GIINIKTKKSDRAGFNGVVTTSYAQGVYPKSNNSLVLNYKTARTNSFFNYSFNDVDYL
                     TNLYAYRKYYDDNKNVAAILQQPAYFSGNVVNNTAKAGLDYSPSARTTVGMQVSGVDI
                     HRSGSNTGHADWLDAAGNLDSSLLTKASPENSFRNGTLNLNARQSLAKNAELKMDIDY
                     LHYQMLGKQNFDNQLLAAGGYDSVFRSSIPTTIDIYSGKVDADAPVGAAGMLQAGVKW
                     TSSHTDNSAT"
     gene            1626..>2844
                     /locus_tag="VKU83_10495"
     CDS             1626..>2844
                     /locus_tag="VKU83_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94032.1"
                     /translation="MILNYLKIAIRSLKKNRAFSAINIFGLAIGLSCCMLIMAYITHE
                     TSYDRQSPDADRIYRIGIHMNQNGGTADYPAVDGGVGPGVRSIYPEVVDVTRFLPADP
                     GYVKVGDRVFKEKKIALCDSNFLRFFSIPLIAGDPKTALAGPGVVVTKELARKYFGDT
                     PALGKSLPIFGQSKITGIIDRLPGNMHFHFDAFVFGNFHSTTWSNIGFYTYLKLAKNA
                     DPTKLEVKFPDLVMKYIVPESVHDMGISLAEARKSASDWHFYLMPVTEIHLYSHSKYE
                     LEPNGDIQYIYIFGALALFILLLACLNFANLATASSAQRAREVGIRKVLGSLRSQLIG
                     QFLAESVVLAFVSMLVALLAVSLLLPLFNELSGKHFEFSFFLRPGNLVFATLLAVAVG
                     TLSGLYPAFFLSAF"
BASE COUNT          598 a          841 c          712 g          693 t
ORIGIN      
        1 taggtggcgg aattatcggt gtgactggac gtccatttga cccccgcctg cagcatccct
       61 gctgcgccga ccggcgcatc ggcgtcgact ttcccggaat agatgtcgat cgtggtcgga
      121 atggagctcc ggaaaaccga atcatagcca cccgccgcca ataactggtt gtcgaaattt
      181 tgcttcccga gcatctgata gtgcagataa tcgatgtcca tctttaattc agcattcttc
      241 gccaaagatt gccgggcgtt tagattgagg gtcccattcc tgaaactatt ttcgggcgac
      301 gccttggtca gcagcgaaga atccaggttt cccgctgcat ccagccaatc cgcgtgaccg
      361 gtgttgctgc cgctgcggtg aatgtccacg ccggatacct gcatccccac cgtggtcctc
      421 gctgaaggcg aatagtccag ccctgccttg gccgtgttat tcacgacatt gccactgaaa
      481 tatgcgggtt gctgcaagat ggcagcgaca tttttattgt catcgtagta cttgcggtaa
      541 gcatataaat tcgtcagata atctacatca ttgaagctgt agttgaaaaa gctgttggtc
      601 cgcgccgtct tgtaattcag cacgaggctg ttattgctct tgggatagac gccctgcgca
      661 taggaagtag tgacaacgcc attgaagccg gcacgatcgc ttttcttcgt tttgatattg
      721 atgatgcccg cattgccggc cgcatcatac cgcgccggcg ggttggagat cagttcaatt
      781 ttcgcaacct gcgcggcatt catgctgctc agcaggttat tcaggtcatc gccggtaaga
      841 tacgtaggtt tatcgtcgat cattaccagc acgcccggct ttccattgag actgatacca
      901 ccgttcctgt caaccgtcac gcccggtgac cgctccagta cctccaggac cgtagcgccc
      961 gtattgacgg gcgaagcgtc cacattgatg attgtccggt ctcttttccg ctcgaccggc
     1021 aatgcccgcg cggtaacggt tgcttcctgc aacgtcgctg ccctcggcgc gaggacgatg
     1081 ctgtctccgg gcggtcctgg caagcggtac tccggcgtca ttttgggctg gtatcccgcg
     1141 ctactgatga gaaaagtata aacgcccttt gggaggctgc caaacagcac gcggccatcc
     1201 tgtcctgcca cggccgtacc ggccggcttc ccgttgcgga ttagtttgac cgtcgcacca
     1261 ttaattccca gatgctgttc attgatgacg accagccaaa aacgtccgtt tccacccgac
     1321 tgacaataca cttcggcagg caccgccgac aacgcgatca ataaacacgc tgcaattttc
     1381 atggtggcgg caaagttcaa ttacattttc gaaaccgccc cgccgctact cccaatccgc
     1441 cgccgttagt ccatatacca ctttttccgg ccggaagaga gaccgtacac tatatgtatc
     1501 gaaatcgcac actgcgtcca gcgggatcca aataatggtt tgatccccaa tctcatcccg
     1561 ttatggtatc tttttggtgg caggaccccg tccggcgcgg gaaccgcgac caaaatattc
     1621 atgcgatgat cttaaactac ctaaaaatcg ccatccgcag tctcaaaaag aacagggcgt
     1681 tttcggcgat caatatcttc ggcctcgcca tcggcctctc atgctgcatg ctgatcatgg
     1741 cttatatcac ccacgaaacg agctacgaca ggcagtcgcc cgatgccgac cggatctatc
     1801 ggattggcat ccacatgaac cagaacggcg gcacggccga ttaccccgcc gtcgacgggg
     1861 gtgttggccc cggtgtcagg agcatttatc cggaagtagt tgacgtcacc cggtttttgc
     1921 cggcggaccc ggggtacgtc aaagtcgggg atcgcgtgtt caaagaaaag aagatcgccc
     1981 tttgcgactc gaattttcta cggttctttt ctatcccgct gatcgccggc gatcccaaaa
     2041 cagcccttgc cgggcccggc gtagtcgtca cgaaagaact tgcaaggaaa tacttcggcg
     2101 atacgcccgc cctcggaaaa tcgctgccca tcttcggcca gagcaagatc accggcatca
     2161 tcgacagact acccgggaac atgcattttc atttcgatgc cttcgtcttt ggcaatttcc
     2221 acagcaccac ctggagcaat atcggtttct acacttatct gaagctggca aagaacgccg
     2281 accccaccaa actggaggtc aaattcccgg acctggtgat gaagtacatt gttccggaga
     2341 gtgtgcacga tatggggatt tcccttgcgg aagccagaaa gtcggccagc gactggcatt
     2401 tttacctgat gcccgttacc gagatacacc tctactccca tagcaaatat gaacttgagc
     2461 ccaacggcga catccagtat atttatattt tcggcgcgct ggccctgttc atcctgctac
     2521 ttgcatgcct gaattttgcg aacctggcga ccgcaagctc cgcgcaacgc gcccgtgaag
     2581 tcggcattcg caaggtcctg ggctcgctca gatcacagtt gatcggccag ttccttgcag
     2641 aatccgttgt cctcgccttt gtctccatgc tggtggccct gctcgcggtg tcactccttt
     2701 tgccgctctt taacgagtta tcgggcaagc atttcgaatt ttcctttttc ctgcgcccgg
     2761 gcaatcttgt cttcgcgacc cttttggcgg tggcggtagg aacgctttca ggcctttatc
     2821 cggcattctt tctatccgcc ttcc
//
LOCUS       DATGKE010000620         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1385911, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000620 DATGKE010000000
VERSION     DATGKE010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385911"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VKU83_10500"
     CDS             <1..930
                     /locus_tag="VKU83_10500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI94033.1"
                     /translation="GEFNDGRVRVITVRGSRRDYFFQKRIAISRKASQLASQFDAVHS
                     LYEDCSYALPPGGFTVTFHVLPGVVRYHEIRQRIFIFLKYHILQRRALRNSASIACVS
                     TNLLAAVPARYRHKARFIPHGVDTGFWDPELASRPTSPVPGEYILCVGSHGLDRPLLT
                     EFIRANPTRHFVIVGLKTRLAPAPNTRYLDEVTDEELRNLYAGATLMIRPLEFATANN
                     SVLEALSMGKTVLASRIPGITDYLTDKTAIFIDTVPDRSLDNIEVRRPDPDLVRQAAI
                     DSFSWEKVLAGYTPLYSENHNHVKPARSIQTEA"
     gene            893..2029
                     /locus_tag="VKU83_10505"
     CDS             893..2029
                     /locus_tag="VKU83_10505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI94034.1"
                     /translation="MSNPPAASKPRRKKRIAVIGLKGLPPFGGAANVGDNLIEQLSGE
                     YDFTIYATASHTHHKGEYKGAHQIVFARFPIKKLNVFYYYIASAFHAVFSGRYDAIHL
                     HHMDGAFILLLLRLRYKVLATSHGLPYNYEKWSKALTPYFKLNEWFQAHLSNHLTVVS
                     RFLVPHYQKLIPDSRISYIPNGVTPMVPATTDLGVSNYILFAAGRIIPLKGVHTMLSA
                     LHLAGFKGKVIILGDHGQLKDYQRKLFELARGLDVEFKGLIKEKALLNAWIAGARLFI
                     FPSTFEAMSMMLLEVACLRTPILCSDITQNTDIFSRDEMLFFKAGSETDLAEKFKWAM
                     DHPDEMRGYASRAYNALMEKFQWTVIARQYAGILENLTEKRVMK"
BASE COUNT          455 a          652 c          516 g          450 t
ORIGIN      
        1 ggagagttca atgacggccg cgtgcgcgtt ataaccgtca ggggcagccg ccgggattac
       61 ttttttcaaa aaaggatcgc gatctcccgg aaagcatcgc agcttgcctc ccagttcgac
      121 gccgtccatt cattgtacga ggactgcagc tatgccctcc cgcctggtgg ttttacggtc
      181 accttccacg ttcttcccgg cgtggtcaga tatcatgaga tcaggcaacg catctttatt
      241 ttcctgaaat accatatcct tcaacgccgg gcgctccgga actccgcttc gatcgcctgc
      301 gtctccacca acctgctggc cgcggtgccg gcgcgttacc gccacaaggc gcggttcatc
      361 ccccatggcg tcgacaccgg cttctgggac cccgagcttg ccagccggcc gacaagcccg
      421 gtcccgggcg aatatattct ctgcgttggc tcccacgggc ttgaccggcc gttgctgacg
      481 gagtttattc gagccaaccc aacgcgccat tttgttattg tcggcctaaa aacccggttg
      541 gcccccgcgc cgaatacccg ctacctcgat gaggtaaccg acgaggagct gcgtaacctg
      601 tacgccgggg cgacgctcat gatccggcct cttgagtttg caaccgccaa caactctgtc
      661 ctcgaggccc tttcgatggg caagaccgta ctcgccagcc gcatcccggg gataacagat
      721 tacctgactg ataagacggc catctttatc gatacggttc ccgaccggtc gctggataat
      781 attgaagtcc ggcgtccgga ccccgacctc gtccgccagg ccgccatcga ttctttctcg
      841 tgggaaaaag tccttgccgg ttataccccg ttgtacagcg aaaaccataa ccatgtcaaa
      901 cccgcccgca gcatccaaac cgaggcgtaa gaaacgcatc gctgttatcg gcctgaaagg
      961 acttccgcct tttggcggcg cggcaaatgt cggcgacaac ctgatcgagc agctctccgg
     1021 ggaatatgat ttcaccatct acgccacggc atcccatacc catcataagg gagaatacaa
     1081 gggcgcccac cagatcgtgt tcgcaagatt tccgatcaaa aaactaaatg tattttatta
     1141 ctatatcgct tcagccttcc acgccgtctt ctcggggcgc tacgatgcga tccatctgca
     1201 tcacatggat ggcgccttta tcctgctgct gctgcgcctc cgctataagg tcctcgccac
     1261 gagccatggc ttgccataca actatgaaaa atggagtaaa gcgctgacgc cttacttcaa
     1321 gctcaacgaa tggttccagg cgcatctctc caatcatctg accgtcgttt caagattcct
     1381 cgtcccgcac tatcaaaagc tcatcccgga ttcccggatc tcttatattc ccaacggcgt
     1441 cacgcctatg gttcccgcca cgaccgatct gggcgtctcc aattatatcc tcttcgccgc
     1501 aggccgcatc atcccgctga aaggggtaca caccatgcta tccgccctcc acctcgccgg
     1561 attcaaaggc aaggtgatca tcctcggcga ccatggccaa ctgaaagact accagcggaa
     1621 gctattcgag ctagcacggg gcctggacgt cgaattcaaa ggcctgatca aggaaaaagc
     1681 cctcctcaac gcctggatcg ccggcgcccg cctcttcatt ttcccctcca cattcgaagc
     1741 gatgtccatg atgttgctgg aagtggcgtg tctgcggacg ccgatcctct gttctgatat
     1801 cacccagaac accgatattt tcagccgcga tgaaatgctt ttttttaaag ccggctctga
     1861 aacggacctg gcggaaaagt tcaagtgggc aatggatcat cctgacgaga tgcgcgggta
     1921 tgcaagccgg gcctacaacg ccctgatgga aaaatttcaa tggaccgtga tcgccaggca
     1981 gtatgccggt atcctggaaa acctgacgga gaaaagggtc atgaaatagg cggctgctac
     2041 atggccgcca acggccggat gaccttgcag ggg
//
LOCUS       DATGKE010000621         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_769247, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000621 DATGKE010000000
VERSION     DATGKE010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769247"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VKU83_10510"
     CDS             <1..306
                     /locus_tag="VKU83_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLI94035.1"
                     /translation="GGLYSMPSNHAANHFSLAAFWYLTIRRLRGKKWTWLWFWAAAVC
                     YAQVYVGKHFPFDIFVGAIFGLLTGWGTSRIFVYWQSRRGHHPPLFGPVLKIRRIEH"
     gene            296..1951
                     /locus_tag="VKU83_10515"
     CDS             296..1951
                     /locus_tag="VKU83_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLI94036.1"
                     /translation="MNIDPFTADWEAWLQKWFLPLLIPAILVNAGGLLIPILEPDGAL
                     YATIAKTMARTGDFVNLFVDGRDWLDKPHFPFWMAAISFRIFGVNGFAYKFPALLFWG
                     AGAWYTFRLAKSLYGRPVAQLSTLIYVSAAHLVISNNDVRAEPYLTGLIIGAVYHFHR
                     RSAGHLLAGSLMAAAALMTKGPFVLLTIGGGFVVDWIIGKDWRQFRDLRWWAAVGMTA
                     LFTLPEIYCLYQQFDRHPEKIVFGHTHVSGVRFFFWDSQFGRFFNTGPIKGSGDPFFY
                     FHTVLWAFLPWSVLLYAAVVHKCRTLRRSAPGDWICAGAALASFALFSLSRFQLPHYL
                     NILFPFFSILTAAWLYGIRRSGTRRFVRIVQTVITVLLPVLLLVVTAFWRFSAWPLVM
                     AGIVGLSVLPFLLFRGGALPVSITRSFWLALVVYSFINFLFYPAILRYQAGTQAGRYV
                     AGTRIPAVYLLREAPVNYSLEFYSRVPVERIPIDSLGPLLRRGPLTVFCPLAFADSLA
                     VRGYPGAVVHSFPNFHVSQLTGDFLDAGTRPRVVQTYVLLNLR"
BASE COUNT          377 a          660 c          622 g          582 t
ORIGIN      
        1 ggcggcctgt attcgatgcc ttccaatcat gccgcgaatc atttcagtct cgccgccttc
       61 tggtatttga ccatcaggcg cctgcgcggc aagaagtgga cctggttgtg gttctgggca
      121 gcggcggtgt gttatgccca ggtctatgtg ggcaaacact ttccgttcga tatctttgtc
      181 ggcgctattt tcgggctgct gacagggtgg ggtacatccc gcatcttcgt ttattggcaa
      241 agccgtcgcg gccaccaccc gcccctgttc ggtccggtcc ttaaaattcg ccgaattgaa
      301 cattgatcca ttcactgctg attgggaagc ctggctgcaa aaatggttcc tccccctgct
      361 catacctgcg atcctcgtga atgccggcgg cctgctgatc cccatcctcg agcctgacgg
      421 cgccctatat gcgacgatcg cgaagaccat ggcgcggacg ggcgatttcg tcaacctttt
      481 cgtggacgga agagactggc tggataaacc gcattttccg ttctggatgg cggcgatcag
      541 cttccgcatc tttggagtga acggcttcgc ctataaattc cccgcgctcc ttttctgggg
      601 tgccggcgcc tggtacacct tccggctggc caaatcgctt tatggaagac cggttgcgca
      661 gctgtcaacc ctgatctatg tctcagccgc ccacctggtc atctccaata acgacgtgcg
      721 cgccgagcct tatctcacgg ggctgatcat tggggcagtc tatcatttcc accggcgttc
      781 tgccgggcac ctgctggccg ggtccctcat ggcggcagcg gccttgatga cgaaggggcc
      841 gtttgtattg ctcaccatcg gtggcggctt cgtcgtcgac tggatcatcg ggaaggactg
      901 gcggcagttc cgggacctgc gctggtgggc tgccgtcggg atgacggcgt tgttcaccct
      961 cccggaaata tattgccttt atcagcagtt tgacaggcat ccggagaaga ttgtttttgg
     1021 tcatacgcat gtttccggcg tccggttctt tttctgggac agccagttcg ggcgtttctt
     1081 caataccgga cccatcaagg gcagcggtga tcccttcttc tatttccata ccgtattgtg
     1141 ggcatttctg ccctggtcgg tcctcttata tgccgccgta gtccacaaat gccggaccct
     1201 ccggcggtcg gcgcccggcg actggatctg cgcgggcgcg gccctggcca gcttcgcgct
     1261 tttttcgctg tcgcgattcc agcttccgca ttatctcaac atcctttttc cgtttttttc
     1321 tatcctcacg gcagcgtggc tgtacggcat ccgccggagc ggtacccggc gctttgtccg
     1381 gatcgtacaa accgtcatca ccgtcctgct gccggtgctg ctcctggtgg tcaccgcctt
     1441 ttggcgtttt tcggcctggc cgcttgtcat ggccgggata gtcggattat cggtgcttcc
     1501 cttccttctt tttcgcggcg gtgccctacc ggtgtccatc acccgaagtt tttggcttgc
     1561 gctggtcgtc tacagcttca tcaattttct tttctatcct gctatccttc gctatcaggc
     1621 cggcacgcag gcaggccgat atgtcgcagg cacccgtatc ccggccgtct acctgctgcg
     1681 ggaagcgccg gtcaattatt cgctcgaatt ctatagccgc gttccggtgg aacggatccc
     1741 gatcgatagc ctggggcccc tgttgaggcg gggtccgctg acggtctttt gtcccctcgc
     1801 ctttgcggat agcctcgccg tccggggcta tccgggtgcg gtcgtccaca gcttccccaa
     1861 tttccacgtt agccagctga cgggcgattt tctggacgcg ggaacccgcc cgcgggtcgt
     1921 gcaaacctat gtcttattga acctcaggtg attatggctg cgcgggcttg tcgccgccgt
     1981 cgcccgccgg ttaccaggcc aaaaaaactt aacattgggt ttgtgccttt aatattaaat
     2041 ttatattatc aaattattaa taaacaaacg ttattcgtat gagtcaacaa gaatcaatcg
     2101 ctcctgacat atcaccgaag aaaaaagtgc gcaaggagat cggcgagaag gtggcgggcg
     2161 cgctggcgga ttacaaacag gagttgggcg aaaaagtatt ggcaacgcat gtcaggcatt
     2221 ttagcaagct tgtcagccgc g
//
LOCUS       DATGKE010000622         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1313803, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000622 DATGKE010000000
VERSION     DATGKE010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313803"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VKU83_10520"
     CDS             <1..699
                     /locus_tag="VKU83_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLI94037.1"
                     /translation="INKEGYESLEEVLGSGLQALDLDAFRKWIKEEDAILLDTRRSDL
                     FVAGFVPGSISIGLDGRFAEWAGSLLPFDKPIVLVTSPGEEKESVVRLARVGFDKVKG
                     YLVGGYPTWQQAGEPIDMIIDVEPDELAMDIPFDENLLVIDVRRESEFAEGHVKEAIN
                     MPLQDLADPGSMANIEERHNLYVHCGGGYRSIIAASLFKRQGIHNLRNIAGGWGRIKD
                     QKGIEIVKEKSVLN"
     gene            complement(703..1368)
                     /locus_tag="VKU83_10525"
     CDS             complement(703..1368)
                     /locus_tag="VKU83_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI94038.1"
                     /translation="MTTLKPGVVLVDDHALLRSGLASLISGFGEFEILFEACDGKDLI
                     RQLQDCRRPDIILLDINMPEMDGFETSCWLRRNFPEIKVLALSMYDTDSSIVRMLRSG
                     VKGYILKDIDAGELRLALESVMEKGYYYTDLVTGKLLHTINHLNEPETRMRRLLTLNE
                     RELEFMKLVCTEWTYKEIADRMYLSPRTIDGYRDALFEKLNVRTRVGLAMYAVKNGIV
                     TLD"
     gene            complement(1490..2395)
                     /locus_tag="VKU83_10530"
     CDS             complement(1490..2395)
                     /locus_tag="VKU83_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLI94039.1"
                     /translation="MYYVVYGSLYVLSLLPMWLLYMLSDGIAFLLYAVIRYRRGVVLN
                     NLAIAFPEKTETERLRIARRFYRNFTDNFIETIKLLSAGPAFLEKRFILDNPELMDHY
                     YDSGRKVQMLLGHCFNWEMANVRMAPHTRYTFIVAYMPVENKAIERLFLRLRGRSGTV
                     LLPATKLSRAILPYRHQQYMLVLVADQAPGGAEHSYWMNFFNRPTPFVRGPERGARAG
                     DIPAVFVRIYKPRRGYYHAEFVTLAEHPAELPEGELTRRFRTMLEDAIRRQPDTWLWS
                     HKRWKYIWREDLGVYWADQGKKLPE"
BASE COUNT          598 a          682 c          675 g          559 t
ORIGIN      
        1 atcaacaaag aaggctatga aagcctggaa gaagtgttgg gatcgggact acaggcgctg
       61 gacctggatg cgttccgcaa atggataaaa gaggaagatg cgatccttct cgatacgaga
      121 cgctcggacc tcttcgtggc aggctttgtg ccgggctcga tcagtatcgg tctggacggc
      181 cgttttgcag aatgggccgg cagcctcctg cctttcgaca agcccatcgt tctcgtgact
      241 tcgccgggag aagaaaaaga aagtgtggtc cgtctggccc gcgttggatt cgataaggta
      301 aaaggctatc tcgtaggcgg ctaccccacc tggcagcagg ccggcgagcc catcgatatg
      361 atcatcgacg tcgagcccga cgagctggct atggatatcc cgttcgacga gaacctgctg
      421 gtgatcgacg tccgacgcga atcggaattt gcggagggac atgtaaaaga ggcgatcaat
      481 atgccgctgc aggacctggc cgatcccgga agcatggcca atatcgagga gcggcacaat
      541 ctctatgtcc attgtggcgg tggatatcgc agcatcatcg ctgcatcgct gttcaaacgc
      601 cagggaatcc ataacctgcg caacatagcc ggcggctggg gaaggatcaa ggatcaaaag
      661 gggatcgaga tcgtgaagga gaagagtgtt ttgaactgat ggctagtcta aagtaacgat
      721 cccgttcttt accgcataca tagccaggcc taccctggtc cggacattca gcttttcgaa
      781 tagggcatcc cggtaaccgt cgatggtccg cggactcaga tacatgcggt ccgcgatctc
      841 tttgtaggtc cactcggtgc ataccagctt catgaactcc agctctcttt cattcagcgt
      901 aagcagccgc cgcatccgcg tttccggctc attcaaatgg tttatcgtgt ggaggagctt
      961 gcccgttacc aggtcggtat aataatagcc cttttccatt accgactcga gcgccagccg
     1021 gagctcgccg gcatcgatat ccttcaggat ataccctttc acgccgcttc gcagcatacg
     1081 cacgatactg ctgtcggtat catacatcga taacgcgagc actttgatct cgggaaaatt
     1141 acgcctgagc cagcaggaag tttcgaaacc atccatctcc ggcatgttga tgtccagcaa
     1201 aatgatgtct ggacggcggc aatcttggag ctgcctgatc aaatccttac catcgcaggc
     1261 ttcgaaaagg atctcgaact cgccaaatcc gctgatcaga ctggcaaggc cgcttcggag
     1321 aagtgcatga tcatcgacaa gcactactcc cggtttaaga gttgtcatcc ggtaaaacgt
     1381 ttaggtttag gtagtcggtc acgctactaa tcagaccacc tttgcacctc aaccgggcac
     1441 ggatgcactt gtagtaaatt tattattatt tccagatcag tggaagtatt tactccggta
     1501 attttttacc ctgatctgcc cagtagaccc caagatcctc ccgccagata tatttccatc
     1561 gcttatgact ccaaagccag gtgtccggct ggcggcggat ggcgtcttcg agcatcgtcc
     1621 ggaaccggcg ggtcagctct ccttcgggca gctccgcagg atgctcagcg agggtaacga
     1681 actctgcatg gtaataacct cgacggggtt tatagatgcg gacgaacacg gcgggtatat
     1741 cgcctgccct ggcccctcgt tccgggcccc ggacaaatgg tgtagggcgg ttgaaaaagt
     1801 tcatccagta ggaatgttcc gctccgccgg gagcctggtc ggctaccagg accagcatat
     1861 actgctggtg gcggtaaggc aggatcgctc tcgacagctt cgtggccgga agcagcaccg
     1921 tccccgaccg gccgcgcaac cggagaaaaa gccgctctat cgctttattc tctacgggca
     1981 tataggcgac aataaaggta taacgcgtgt gcggcgccat tctcacattg gccatctccc
     2041 agttgaaaca atgtcccaga agcatctgta ccttccggcc cgaatcgtaa taatgatcca
     2101 tcaattccgg attatcgaga atgaatcgtt tttcgagaaa ggcaggaccc gcactcagca
     2161 gcttgatcgt ctcgataaaa ttatccgtaa aattccggta gaaacgccgg gcgatgcgta
     2221 gccgctccgt ttcggtcttt tcgggaaagg cgatcgccag attgttcagg accacccccc
     2281 gccggtaacg gatcacggca tacagcagaa aagcaatccc gtccgacagc atgtataata
     2341 gccacatcgg tagcagcgag agcacataga gcgacccgta gacaacgtaa tacatagggt
     2401 taggtgttga cccaattttt ggcttcctgc acgatatgat gcagttcgat cttgacgtga
     2461 aatttattgc gcagatgccg ggccagcgca tcggccgcat agacgctccg gtgc
//
LOCUS       DATGKE010000623         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_982203, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000623 DATGKE010000000
VERSION     DATGKE010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982203"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VKU83_10535"
     CDS             <1..552
                     /locus_tag="VKU83_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLI94040.1"
                     /translation="VPRGGVPVAYTVAKELGLPLDLILTKKIGHPVNREYAIGAVSLT
                     DAFVIPHRGVSQFYIDQEIKTIRQRLKEMYRKFMGDKEPESLSGKTVIVIDDGIATGN
                     TLLGTINILRKNNPAKIVIAVPVASADAVRKLSEVADELIVVLIPEEFYGVGAFYENF
                     EQVTDEEVMYYLDKWRREIKKAG"
     gene            586..1092
                     /locus_tag="VKU83_10540"
     CDS             586..1092
                     /locus_tag="VKU83_10540"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLI94041.1"
                     /translation="MIVIVLGLPGSGKSYFASRFSKLIHADYINSDRVRKSIFPARSY
                     SEKEKASVYDEMFELMRQAIKQKKDLVLDATFYKNDIRRKFIDEAGGDLVLIEVAAEE
                     AIIKERLKQKREDSDADFEVYQLIKKRWEPLQGHRLILISFNNNIEEMLHEAMVYLKL
                     RNDKRAYC"
     gene            complement(1117..1311)
                     /locus_tag="VKU83_10545"
     CDS             complement(1117..1311)
                     /locus_tag="VKU83_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94042.1"
                     /translation="MSKTKKPQGPAEVPQPDRDPEVTPVTTPGSPMEPEEEPEIVPEN
                     EPEEPAGPAPAEIPAPGKGN"
     gene            complement(1322..1996)
                     /locus_tag="VKU83_10550"
     CDS             complement(1322..1996)
                     /locus_tag="VKU83_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLI94043.1"
                     /translation="MDFRCDREVTIPVGKVTLKGELIIPAKGTAIVIFSHGSGSSRMS
                     PRNRKVARQLQEMGFGTLLLDLLTPLEDEQFSNRFDIELLTRRLIGATKWVSTLSETL
                     NCCLGYFGASTGAASALKAAAELPQVNAIVSRGGRPDLAMDALSKVKAPTLLIVGSLD
                     YEVLELNKMAYQQLTGEKKLEVVSGASHLFEEYGALDKVAEMAGRWFSRYLLPKHIPG
                     HLKATR"
     gene            2234..3046
                     /locus_tag="VKU83_10555"
     CDS             2234..3046
                     /locus_tag="VKU83_10555"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLI94044.1"
                     /translation="MNNLTQRTITGCILGLLITLAVSWNAYSFILLILVIDLLSLLEF
                     YRLFRDAGLSPRKISGLISSISGLLTCVLVMSKICGPKILLINIPALFGIFLSELFRQ
                     EQNPFHNLAFTFLGNLCITIPLCFFIGLAFLPVGSGIYHSRIVVGYFFLLWASDSGAY
                     FIGKAFGTHALFKRISPRKTWEGSLGGAVCALFVAYIVSRYVAIPDATAWMIMALIIV
                     ITGTFGDLIKSMMKRSLNLKDSGTILPGHGGMLDRFDTLLGSAPFVFCYLVL"
     gene            complement(3052..3927)
                     /locus_tag="VKU83_10560"
     CDS             complement(3052..3927)
                     /locus_tag="VKU83_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI94045.1"
                     /translation="MNTILIATDFSEASRNGAAYGFQLARAFDSKIILFNAYMAMPVT
                     VTEIPANVIADDLNKYTQQQLQLEARSIIGRSKVTVETICKEGPAADNILEIAKEKHA
                     GLIVAGMKEGDKGVRKMLGSTATELARRTRIPMVIVPEKAKYTDISSIALANQSDADP
                     YADNHLLDLIQEIAGKFHSKLYLVRVAKNRVKAAYETLNRPFKLIRMMESLDPAYGAI
                     EGKRIPEALNHFIEGYHIHMLAMLPHQHSLLERWFMKSATRSMVFETRIPLLILPDLH
                     IEKKDHKRFQKEAWR"
     gene            complement(3927..>5228)
                     /gene="glgP"
                     /locus_tag="VKU83_10565"
     CDS             complement(3927..>5228)
                     /gene="glgP"
                     /locus_tag="VKU83_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HLI94046.1"
                     /translation="KLYDSNPETRIAAAILLGTGGARLLENLEWAPEVYHLNESHGLP
                     LAFYLYDKYKSIDEVKKRLVFTNHTPEEGGNPTTNILLLDKLGFFNNLPLTEVRAIAQ
                     IENDSLNHTLTALRLSRVANAVSKLHLQTLCSMWKKYEGICPLKSITNAQNFSYWADQ
                     AMYKAISLSDEKALKERKSACKEELFQIVADENGEIYDRSIFTIVFAKRFAGYKRADL
                     LCQDLGRFERLVSNRDRPVQIIWAGKPHPTDYTAIGVFDKIVHLCKTYSNCSVLVGYE
                     LKLSKLLKQGADIWLNVPRITHEASGTSGMAAAMNGAVNVGIPDGWFPEFARDKINSF
                     VIPPSDPALQDHQQDEADARSLYELLEKEVIPTYYDYPSRWLSMMKSSMQDVIPYFDS
                     NRMASEYYEKLYAGKEQADSINLNGSTGNAKAATTISLKSE"
BASE COUNT         1258 a         1241 c         1234 g         1495 t
ORIGIN      
        1 gttcccagag gtggtgtgcc ggttgcttat accgtggcca aagaactggg gctgccgctc
       61 gacctgatcc ttaccaaaaa gatcggccac ccggtgaaca gggaatatgc catcggggcc
      121 gtaagcctga ccgacgcctt cgtcatcccc catcgtggag tgtcgcaatt ttacatcgat
      181 caggagatca aaacgatacg gcaacgattg aaagaaatgt accggaaatt tatgggcgat
      241 aaggagccgg aatccctgag cggtaaaacg gtgattgtga tcgatgacgg gatagccact
      301 ggaaatacgt tgctgggcac cataaacatc ctgcggaaaa ataaccctgc taaaatcgtg
      361 atagccgtcc cggtagcttc cgctgacgcc gtcaggaaat tgtcggaagt agcggatgaa
      421 ttgatcgtcg tcttgatacc ggaagaattt tatggagtgg gggctttcta cgaaaatttt
      481 gaacaggtaa ccgatgaaga agtgatgtat tacctcgata aatggagaag agaaataaag
      541 aaagcgggat gaagtcccag agtgttcaat ttaaagcgat tttttatgat tgtcatagtg
      601 ttgggcttgc cggggtcggg gaaaagctac tttgcctccc ggttttccaa gctgatccat
      661 gcggattata tcaatagcga ccgggtaaga aaaagcatat tccccgccag gtcctattct
      721 gaaaaggaaa aagcatccgt ttatgatgaa atgtttgaat tgatgcgaca ggcgataaaa
      781 cagaaaaagg acctggtcct ggatgcgaca ttctataaaa atgatatccg caggaaattt
      841 attgacgaag ccggaggaga cctggttctc attgaagtgg cggccgaaga ggccattata
      901 aaggaaagat tgaagcaaaa aagagaagac agcgacgccg attttgaagt atatcaactc
      961 ataaaaaaac ggtgggaacc tttgcagggg caccgcttaa tattgatttc tttcaataat
     1021 aacatcgagg agatgttgca cgaggccatg gtttatctca agttgagaaa tgacaaaaga
     1081 gcctattgct aaatataggt agctcgatgt acagttttaa tttcccttcc cgggtgccgg
     1141 aatttctgcc ggagcgggcc ctgccggttc ttccggctca ttttcaggaa cgatctccgg
     1201 ctcttcttcc ggctccattg gggatcctgg cgtcgtaaca ggcgttactt ccggatccct
     1261 atccggttga ggcacttcag cgggaccctg gggtttcttt gttttgctca tgggattaaa
     1321 attacctggt tgcttttaag tggccgggga tgtgctttgg cagcaaatac ctgctgaacc
     1381 atctgccagc catttcagcg accttatcca gtgcgccgta ttcttcgaat aaatgagagg
     1441 cgcctgatac cacttccaat tttttctcgc ccgtcagttg ctgatatgcc attttattca
     1501 gttccaacac ctcgtaatca agactgccga cgatcagcaa agtaggcgcc ttgactttgc
     1561 taagcgcatc catggctaaa tcgggcctgc cgccacggga tacgatggca ttgacctgtg
     1621 gaagctcagc agcggctttt agtgcagacg cagccccggt gctcgcgccg aaataaccga
     1681 gacaacaatt cagcgtctcg ctcaacgtac tcacccactt cgtagctccg atcaatcgcc
     1741 gggtaagcaa ttctatatca aaccggttgg agaattgttc atcttccaac ggggtaagca
     1801 ggtccagtaa gagcgtccca aagcccatct cttgcaattg tcttgctact tttctgttcc
     1861 gcgggctcat gcgactgctg ccgctgccat gagagaaaat gacaattgcc gttcccttcg
     1921 ccggaatgat caattctcct tttagggtaa ctttccccac cgggatcgta acttccctat
     1981 cgcaccgaaa gtccatatta aaatgtttgg ctcaaattac cggaaattcc ccctcgttcg
     2041 gatgaccgga gtcacttcat tgattgatcc tgaacggcat tgcctcccca tttcaaatac
     2101 ggcgcttcct cttgatcacg atcaatactc ctggtgacgg ccgtcatcgt ttttccctgg
     2161 aaaagctttt accttcacta ccaacgtcga ccgggggcag gctttcgagc ctgccttttt
     2221 tgctaaagca gttatgaata atctaacgca gcggaccatc accggatgta ttttagggct
     2281 cctcattacg cttgcggtaa gttggaacgc ttatagtttt attttattga tactggtcat
     2341 cgatctgctc agcttactgg aattttaccg gttatttcgt gatgccggcc tgtcaccgcg
     2401 gaaaatttcc ggccttattt cgtccataag cgggctcctt acctgtgtct tggttatgag
     2461 taaaatatgc ggaccgaaga tcttattaat taatattcct gcactattcg ggatttttct
     2521 ttctgaactt ttccggcagg aacaaaatcc ctttcacaac ctggccttca cctttttagg
     2581 gaatctttgt ataaccattc ccctgtgttt ttttatcggg ttggccttcc ttccggtcgg
     2641 atcagggata tatcattcca ggatagttgt tggatacttc ttccttcttt gggcaagtga
     2701 ttccggagca tacttcattg gtaaggcatt tggcacgcat gctttattta aacgcatttc
     2761 accgaggaag acctgggaag gaagcctggg aggcgcggtc tgcgccctgt ttgttgctta
     2821 cattgtttcc cgttacgtag cgattccgga tgcaacggcc tggatgatca tggcgctcat
     2881 aatagttatt acgggaacat tcggcgatct tatcaaatcg atgatgaaaa gaagcctgaa
     2941 cctgaaggat tccggaacca ttttacccgg acatggcggg atgctggacc gttttgatac
     3001 tttattgggc tccgcgccgt tcgttttttg ctatttggtt ctatgacccg gctatcgcca
     3061 cgcttccttt tgaaatcgtt tgtgatcctt tttttctata tgcaggtcag gcaggatcag
     3121 taatggaatc cgggtctcaa aaaccatgga gcgcgtggcg cttttcatga accatctttc
     3181 cagcagggaa tgttggtgag gcagcatcgc cagcatatgg atatgatatc cctcgataaa
     3241 gtgatttaat gcttcgggta ttctcttgcc ttctatggcc ccataggcgg ggtcgagcga
     3301 ttccatcatc ctgatcaatt taaacgggcg attgagcgtt tcatatgccg ctttcacccg
     3361 gtttttagcg acccggacca ggtacagttt tgagtggaac tttcctgcta tctcttgtat
     3421 caaatccagc aggtgattat ccgcataggg atccgcatcg ctttgattgg ctaaggcaat
     3481 ggacgagata tcggtgtatt ttgctttttc aggaactatg accatcggaa tcctggttct
     3541 tcttgccagt tccgtggctg tactgcccag catctttcgc acgcctttat cgccctcttt
     3601 catccctgcg acgattaagc cggcgtgctt ttccttagcg atttccaata tattatctgc
     3661 cgcagggccc tccttgcaga tggtttcgac ggtcacttta ctccggccaa taatggacct
     3721 ggcttccagc tgcaattgtt gttgcgtgta tttattcaaa tcatccgcta tgacatttgc
     3781 cggaatttct gtaacggtaa caggcatcgc catgtaagcg ttgaaaagta ttatttttga
     3841 gtcaaaggcc ctggctaatt gaaagccgta ggcggcgcca ttccgggaag cttccgaaaa
     3901 atcagtggcg attaatatcg tgttcattat tcacttttta aagatattgt tgtggcggct
     3961 ttagcgtttc ccgtgctgcc gtttaagtta attgagtcag cttgctcctt tcctgcatac
     4021 agcttttcgt agtattccga agccattcga ttgctgtcaa aataagggat tacatcctgc
     4081 atactgcttt tcatcatcga cagccaccgg gaaggataat cataatacgt cgggatcact
     4141 tccttttcca ggagttcata taaacttctc gcgtctgcct catcctgctg gtgatcctgt
     4201 agagccggat cggaaggggg gataacaaag ctgttgatct tatccctggc gaattccgga
     4261 aaccatccat ccggaatacc gacgtttacc gcaccgttca tggcggccgc catgccgctg
     4321 gtaccggaag cttcgtgcgt gatcctggga acgttcagcc agatatctgc gccttgtttt
     4381 aataattttg acaatttaag ctcatatcct accaataccg aacaattgga ataagtcttg
     4441 caaaggtgga caatcttatc aaaaacgccg attgctgtgt aatccgtagg atgaggttta
     4501 cccgcccaga tgatctgaac gggccggtcc cggttgctca ccaatctctc aaagcgtccc
     4561 aaatcctgac aaaggagatc agccctctta tatccggcaa agcgtttagc aaagacaatg
     4621 gtgaaaattg atctgtcgta tatttccccg ttctcatcgg cgactatttg aaataattct
     4681 tccttgcagg cagatttcct ttctttcagg gctttttcgt cactcaggga tatggcttta
     4741 tacatggcct gatcggccca gtagctgaaa ttctgggcgt tagtgatgga tttcaggggg
     4801 catatccctt cgtatttctt ccacatgctg catagggttt gcagatggag cttcgataca
     4861 gcattggcaa cacgggataa gcgcaaggca gtcagtgtat gattgagtga gtcgttctca
     4921 atttgcgcaa ttgctcttac ttctgtgagc ggtagattgt taaaaaaacc taatttatcc
     4981 agcagcagaa tattcgttgt aggattgccg ccctcttcgg gagtatggtt ggtaaaaacc
     5041 aatctttttt tcacttcatc tatgctttta tacttatcat ataagtaaaa tgccagcggt
     5101 aacccgtgcg attcattaag atggtagact tcaggggccc attccaaatt ctccagtaac
     5161 ctggcgcctc ccgtacccaa caatatggcg gctgcaatcc ttgtttccgg gttagagtca
     5221 tataattt
//
LOCUS       DATGKE010000624         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1101590, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000624 DATGKE010000000
VERSION     DATGKE010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101590"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VKU83_10570"
     CDS             complement(<1..455)
                     /locus_tag="VKU83_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLI94047.1"
                     /translation="MYYSYVKALHIIFIVTWFSGLFYMVRLFIYHTEANERPEPDRSI
                     LQKQFRLMIRRLWLGITWPSAILTLIFGPWMMILLGGMPAWLAIKLGFVAGLYAYHFS
                     LQAIYREQMKGIFRFSSQQLRIWNEVATIFLVAIVMIAVVKQNMSWAWGL"
     gene            complement(472..1524)
                     /gene="hemH"
                     /locus_tag="VKU83_10575"
     CDS             complement(472..1524)
                     /gene="hemH"
                     /locus_tag="VKU83_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLI94048.1"
                     /translation="MTKAGQRAILLMNLGSPDSTATRDVHTYLMEFLMDKRVIDYPWL
                     FRKMLVGGLIVPFRSAKSAEAYKTIWTREGSPLIVISQQLQAALQELVEEPVEIAMRY
                     GNPSVEEAFHRLLRQMPQLKEVIAIPLYPHYAMASYETAVEFAQATHRKGKYSFALRF
                     IRPFYNDAGYLNALSEHIRPYLQREYDHILFSYHGVPARHVRKSDPTGKHCLQSATCC
                     ETASAAHATCYRHQCFATTREVVRRLNIPQSKHSNSFQSRLGKGWLEPFTDIRLEQMP
                     GEGIRKLLILCPAFVSDCLETLEEIAIRGKETFLNAGGTEYTMIPCLNAEPLWVRTLA
                     GWVNDYAKGERTMILD"
     gene            1681..>2500
                     /locus_tag="VKU83_10580"
     CDS             1681..>2500
                     /locus_tag="VKU83_10580"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94049.1"
                     /translation="MGNPTADISRFYAIGINYKKTDATIRGQFAIGHDQYQRLLALAS
                     SHHVTELFVLSTCNRTEIYGFADSARQLSDLLCSAAKGDRAAFREMAYVKRGSEAIEH
                     LFEVSAGLDSQILGDYEIVGQIKQAVKFSREHGYIGPFLERLVNCVLQSSKAVKNQTE
                     LSGGTVSVSFAAIQYIRERYVDLSGKKILLIGIGKIGGNTCRNMVDYLGTRNITLMNR
                     TERKAAALAAETGVSYASLDRLAEHAGDSDIILVATHADHPTLPGRHLQGRGEKL"
BASE COUNT          632 a          688 c          640 g          540 t
ORIGIN      
        1 aggccccagg cccagctcat attctgctta accactgcta tcatgacaat tgcaaccaga
       61 aagatcgtag ccacctcatt ccagatacgc agctgctggg aagagaaccg gaagataccc
      121 ttcatttgct cccggtagat cgcctgaagc gaaaaatgat aagcataaag gcctgccaca
      181 aatccgagtt ttatcgccag ccacgcaggc atccccccca gaaggatcat catccaggga
      241 ccaaagatca gcgttaggat ggcgctgggc cacgtgatgc ccagccaaag ccggcggatc
      301 attaaccgga attgcttttg cagaatgctc cgatccggct cgggtcgttc attggcctcc
      361 gtgtggtata tgaacaacct taccatataa aaaagaccgc tgaaccaggt gacaataaaa
      421 atgatgtgca gcgccttgac ataactataa tacataggaa gagtttacaa ttcaatccag
      481 gatcatcgtc ctttccccct tcgcataatc gttgacccag ccggccaatg ttcgtaccca
      541 tagcggttcg gcattcaaac aagggatcat cgtatattcc gttccgcccg cgttcagaaa
      601 ggtctctttt ccccggatgg ctatctcttc gagcgtctcc aggcaatcgc tcacaaaagc
      661 aggacaaagg atcagcagtt tcctgatccc ttctcccggc atctgttcca gacgaatatc
      721 cgtaaagggt tccagccagc ccttgccgag ccgggattgg aaggaattgc tgtgtttgct
      781 ttgcggtatg ttcagccgcc gcacgacctc cctggtagtc gcaaaacact ggtgcctgta
      841 gcaggtggca tgcgccgccg aagccgtctc gcaacaggtg gcagattgca gacaatgttt
      901 gccggtggga tccgatttac ggacgtgtcg cgccggcaca ccgtgatagc tgaagaggat
      961 atgatcgtac tcgcgctgaa gatacgggcg gatatgttcg ctcagggcat tcagatagcc
     1021 cgcgtcgtta tagaacggcc ggatgaaccg taacgcaaag gaatattttc ctttgcgatg
     1081 cgtcgcctgc gcaaactcca ccgccgtctc ataagaagcc atggcataat gcggataaag
     1141 cggaatggcg atgacctctt tcagctgcgg catctgcctg agcaaccggt ggaaagcctc
     1201 ttctacagac ggattgccgt agcgcatcgc tatttcgacc ggctcttcga ccagctcctg
     1261 caatgccgcc tgcagctgct gactgatcac gatcagcgga gatccctccc tggtccagat
     1321 cgtcttatag gcttcagcgg atttggctga acggaaaggg acaatgagcc cgcccaccaa
     1381 catcttacgg aacagccacg ggtaatcgat cacccgcttg tccataagaa attccattaa
     1441 ataagtgtgt acatcccggg tagccgtcga atccggggag cctaaattca taagaagaat
     1501 ggcgcgctgg cctgctttcg tcatagcgag cacaaaggta gaccctagcg gcgcattacc
     1561 gccaaaaatt taaaatgaca ttctaatgac agaaaaaagc tacatatcgc agccgccgaa
     1621 agtacttttg cgtttagtat tgacggacca agggccgtcg cacatattag actcaagcac
     1681 atgggcaatc cgaccgcaga tatttccagg ttctatgcaa tagggatcaa ttacaagaaa
     1741 acggatgcaa cgatccgggg gcagttcgcc atcggtcatg atcaatatca aagacttctc
     1801 gccctcgcat cttcacacca tgtaacggaa ttattcgttt tatccacctg taacaggacg
     1861 gagatctacg gcttcgccga ttccgcccgg cagctgtcgg acctgttgtg ttctgccgcc
     1921 aaaggtgatc gggcagcttt cagggagatg gcgtatgtaa agcgcggatc ggaggcgatc
     1981 gaacacctgt tcgaggtctc tgcaggactc gactcccaga tcctgggcga ctacgagatc
     2041 gtgggtcaga tcaaacaagc ggtgaaattc tcccgtgagc atggttatat cggccccttt
     2101 ctggaaaggc tggtcaattg tgttttgcag agttctaaag ccgtaaagaa tcaaacggag
     2161 ctgagcggag ggaccgtatc ggtatccttt gccgccatcc aatatatccg cgagcggtac
     2221 gtagacctca gcggtaaaaa gatcctgctc atcgggatcg gcaagatagg cggcaacacc
     2281 tgcaggaaca tggtcgacta tcttggcaca cggaacatta ccctgatgaa ccgtaccgag
     2341 agaaaagccg ccgcactggc cgccgaaacg ggtgtcagtt atgcctcact cgaccggctg
     2401 gccgaacatg ccggcgattc ggatattatt ctcgtagcga cccacgcgga tcatccgacc
     2461 ttgcccggca gacatctgca gggccgcggc gaaaagctgg
//
LOCUS       DATGKE010000625         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1386090, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000625 DATGKE010000000
VERSION     DATGKE010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386090"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VKU83_10585"
     CDS             <1..615
                     /locus_tag="VKU83_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94050.1"
                     /translation="RMSEWYNHPSVYIRSLSTRGSLGGLSPRIIGITELLKAAPFDTI
                     IIETVGVGQNETEIAGLADCTLVVLAPEAGDDIQSMKAGLMEIADVFVVNKSDRPDAD
                     VFVGHLRAMQAPAFRRESREIPILKTVATERRGIDELAAAIRQSLQDPDSSEKRVRLL
                     AERAYVLIQQKRMAGISKAGLAEKIEAAVRAGGFSLRAFVAAFT"
     gene            complement(608..1543)
                     /locus_tag="VKU83_10590"
     CDS             complement(608..1543)
                     /locus_tag="VKU83_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI94051.1"
                     /translation="MNDTPLVSVVLVTYNGEAYLEEQLRSVLAQTYRSLEIIAIDDGS
                     ADGTVGILRKYAAVDSRIKVVVNDRNLGFIRNFEKGCKLAAGKFIALCDQDDYWVPEK
                     IGRMVEAIGDYPMVYCDSELCGEDLQPLGKRISDLVHYQSFHDCRQLCVFSRMYGHAT
                     LITRELFVKASPFLDEVPHDGWLAFHATLYGGVRYLPEVLVKYRQHAANVYGVVGRKR
                     RKETASSRTEKRKKELARIRIRMAAYCAACPDFFVPQKRLLRALVSSYRNFYPWNDIR
                     RVLLFLGNCRLLLAVKDYSMPRKYLFCIKMLVKIK"
     gene            complement(1536..1760)
                     /locus_tag="VKU83_10595"
     CDS             complement(1536..1760)
                     /locus_tag="VKU83_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94052.1"
                     /translation="MKKLTAILLLAFLMLMADVPAAKAQCSICTRTAQQLGERPAKAL
                     NKGIIYLAFAPLAIFGIIGYRWWRSEKVHE"
     gene            complement(1865..3679)
                     /gene="typA"
                     /locus_tag="VKU83_10600"
     CDS             complement(1865..3679)
                     /gene="typA"
                     /locus_tag="VKU83_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HLI94053.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKILHATHVFRDNQETGELIMDNN
                     DLEKERGITIFSKNAAVEYKGVKINVIDTPGHSDFGGEVERVLKMADGVLLLVDAFEG
                     PMPQTRFVLQKALQLHLKPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLDFPT
                     FYGSGKNGWFNSKNEPCGDITPLLDGILKYVPAPVVGDGPLQMQITSLDYSSFLGRIA
                     VGKVARGVIREGQTVALMQPGGVMRRSKVKELYTFMGMGKKKAAEVEAGDICAVVGLE
                     EFNIGDTIADAENPEALPVISVDEPTMSMLFGINNSPFYGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDTDSADSFLVFGRGILHLGILIETMRREGFELTVGQPQVLVKEINGKK
                     NEPYEILVVDVPAEFSGKVIDMVTQRKGELMVMESKGEMQHLEFEIPSRGLIGLRSGM
                     LTNTAGEAVMAHRFLEYKPWKGLIPGRNNGVLLSKNQDKTTAYSIDKLQDRGTFFVDP
                     GEDVYVGQIIAEHIKPGDLIVNATEGKKLTNMRASGSDDAVRIVPKKQMTLEECMEYI
                     QQDECIEVTPKKIRLRKVILDEEERKRTQRAMKTEEAR"
BASE COUNT          811 a         1178 c          952 g          847 t
ORIGIN      
        1 cggatgagcg aatggtataa ccatccctcg gtgtatatcc gcagcctgtc cacccgcggg
       61 tccctcggcg gccttagccc ccgcatcatc gggatcaccg aactgctgaa agcagcgccc
      121 ttcgatacca tcatcatcga aaccgtcggc gtcggccaaa atgaaaccga gattgccggc
      181 cttgcggact gcacgctggt cgtacttgca ccggaagccg gtgacgacat ccagtcgatg
      241 aaggctggcc tcatggaaat tgccgatgtc tttgtcgtca ataaaagcga ccggccggat
      301 gccgacgttt tcgtcgggca tctccgcgcc atgcaggccc ccgcgttccg gcgcgagtcg
      361 cgcgagatcc cgatcctgaa aaccgttgca acggaacgcc gcggcatcga tgagctggcc
      421 gcggccatca ggcaaagcct gcaagatccg gacagttccg aaaaacgggt ccggctgctc
      481 gccgaacgcg cgtatgtcct gatccagcaa aaaagaatgg ccggcatcag caaggccgga
      541 ctcgctgaaa aaatcgaagc ggccgtgaga gccggcggat ttagcctccg cgccttcgta
      601 gcggccttta cttgatcttc acgagcatct tgatgcaaaa aaggtatttc cgcggcatgg
      661 agtaatcctt caccgccagc aatagccggc agttgccgag gaacagcagc acccggcgaa
      721 tgtcattcca gggataaaaa ttccgataac tgctgaccag cgcccgcaaa agacgttttt
      781 ggggaacgaa aaaatcgggg catgccgcgc agtaagccgc catccggatg cggatccgcg
      841 ccaactcctt tttccttttc tccgttcggg atgacgccgt ttctttgcgc cgcttcctcc
      901 ctacgacgcc ataaacattc gccgcatgct ggcggtattt gaccagtacc tcgggcagat
      961 agcgaacgcc cccatacagg gtagcatgaa aagccagcca gccgtcatgc ggcacttcgt
     1021 ccaggaaagg actcgccttg acgaacagct cccgcgtgat gagtgtcgca tggccataca
     1081 tccgggaaaa aacacaaagt tgcctgcagt catggaagga ctggtaatgc acgagatcgg
     1141 aaatccgctt tccaagcggt tgcaaatcct cgccacaaag ctccgaatcg caatagacca
     1201 taggatagtc accgattgct tcgaccatcc ggccgatctt ttcgggaacc cagtaatcgt
     1261 cctgatcgca aagtgcaata aactttccgg ccgcgagctt gcagcctttc tcaaaattcc
     1321 ggatgaagcc caggttccgg tcgttgacca ctaccttgat ccggctatcg accgcagcat
     1381 atttacgcag gattccgacg gttccgtccg cgctgccgtc atcgatcgca atgatctcga
     1441 gggacctata cgtctgcgct aaaacggagc gaagctgttc ttcaagatag gcttcgccgt
     1501 tatacgtaac gagcaccacg gaaactaacg gagtatcatt catggacttt ctcgcttctc
     1561 caccaccggt acccgatgat gccgaatatg gccaggggag caaacgcaag atagataatt
     1621 cccttgttca gcgcctttgc cggccgctcg cccaattgct gggccgtccg ggtgcagatc
     1681 gaacactggg cttttgcggc cgggacatcg gccatcagca tcaggaacgc caacaataag
     1741 atcgcagtta actttttcat gactgccgac tttttcatga ctgccggttg aaccctaaaa
     1801 ttacaacatt ccggtcattc ggcggaaacc gcctgcctgc ccacggcggc ccggcccgcc
     1861 gccgttaccg cgcctcttcc gtcttcatgg cgcgctgggt ccgcttccgt tcctcctcat
     1921 cgaggatgac cttgcggagc cggatctttt tcggcgtgac ctcgatacac tcatcctgct
     1981 ggatatattc catacattcc tccagcgtca tctgcttttt ggggacgatc cgcacggcat
     2041 catcgctgcc gctggcccgc atattggtaa gtttctttcc ttctgtcgca ttcacgatca
     2101 ggtcgccggg tttaatatgc tcggcaatga tctgaccgac gtagacatcc tctccgggat
     2161 cgacaaaaaa ggtaccccgg tcctggagtt tgtctatcga ataagccgtg gtcttgtcct
     2221 ggttcttgga taataagacc ccattgttcc ggccggggat aaggcctttc cagggtttgt
     2281 attccaggaa gcggtgggcc atcaccgctt ccccggcggt attggtcagc atgccgctcc
     2341 gcaagccgat caggccgcgg gaagggatct cgaattcgag gtgctgcatc tctcctttgc
     2401 tttccatcac catcagctcc ccttttcgtt gagtgaccat gtcgatcacc ttgccgctga
     2461 actcggcagg aacatccacg accaggatct cgtacggctc attctttttg ccgttgatct
     2521 cttttaccag gacctggggt tgtcccacgg taagctcgaa accctcccgg cgcatcgtct
     2581 cgatcaggat gcccaggtgc aggataccgc ggccaaagac caaaaagctg tccgcgctat
     2641 cggtgtcttc cacccgcaat gccaggttct tttccgtttc cttcatcagc cggtcccgca
     2701 gatggcgcga ggtcacgaac ttgccgtcct tcccgtaaaa aggcgaatta ttgatgccaa
     2761 acaacatgct catggtcggc tcgtcgacac tgatcaccgg caacgcctcc ggattctccg
     2821 catccgcgat cgtgtcgccg atattaaact cttccagtcc taccacggcg cagatgtcac
     2881 cggcttcgac ctcggcggcc ttcttcttgc ccatgcccat aaacgtatag agctccttga
     2941 ccttcgaccg ccgcatgacg ccgcccggct gcatcagtgc aaccgtctgg ccttccctga
     3001 tcactccgcg ggcaaccttg cccacggcga tccggcccag aaaggaagaa taatccagcg
     3061 acgtgatctg catctgcagc ggaccgtcgc cgaccaccgg cgccggaaca tatttcagga
     3121 tgccgtccaa aagcggcgta atgtccccgc aaggttcgtt cttgctgttg aaccacccgt
     3181 tcttcccgct cccgtagaaa gtgggaaagt ccaattgctc ctcggttgca tccaggttga
     3241 agaacagctc gaagaccgca tcgtgcacct catccggccg gcaattcggc ttgtccacct
     3301 tgttgatgac gacgatcggc ttcagatgaa gctgcagggc cttctgcagg acgaaccgcg
     3361 tctgcggcat cggcccttca aaggcgtcca ccagtaacaa tacgccgtcg gccatcttca
     3421 gaacgcgctc cacctcgccg ccgaagtcag agtgacccgg agtatctata acattaattt
     3481 taacgccctt gtattcgacc gccgcattct tgctgaagat cgtgataccc cgttcctttt
     3541 cgaggtcgtt attgtccatg atcagttcgc ccgtctcctg gttatcccgg aacacatggg
     3601 tcgcgtgcaa gatcttatct accagggtcg tcttgccatg gtcaacgtga gcgatgattg
     3661 caatatttct aatctccata attttgtcgc atgtaggctc ccgccccaat ttcggccgca
     3721 aaattatgcc aaaactgccg gatttcctag ctaaaacgct cggtgcgctg ccatttccca
     3781 aaaattaa
//
LOCUS       DATGKE010000626         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_857022, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000626 DATGKE010000000
VERSION     DATGKE010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857022"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VKU83_10605"
     CDS             <1..921
                     /locus_tag="VKU83_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLI94054.1"
                     /translation="LIATQKLIVSQQKNLDRAVAFQTVATARAKTGLNPGVDSSLANA
                     EVSNARIALTNAIEAEQEQANALGQLMQVPVPDSNNFALDSAFISRLPASLNNAPAQP
                     LQNHPLLKYFKDRVNLSQEQSNYLKTQQYPTLSLFGVGQDRGSGFATNYGPGNPGAYT
                     TSYLKGVSFNTANYLIGIGVYWNFTSPFRVREQVAAQRWTTAGLQEEYGLVSQELQAQ
                     MVLAQSRIGNAVKNYQEAPVQVKAASDAYLQKETLYKNGLATIVDVTTAAFILNQAET
                     QRDVIDTNVWQALLYKAASTGDLGLFINEF"
     gene            931..>2298
                     /locus_tag="VKU83_10610"
     CDS             931..>2298
                     /locus_tag="VKU83_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI94055.1"
                     /translation="MGLIRLALRKPITIVVLVAGLLFFGIGAVRSIKIDIFPKLDLPV
                     IYISHPYGGFTPDQMESYFGKQYVNLLLYVGGVKSIESRNIQGLTLIKLTFYEGTNMA
                     QAASEVTGYITRAQSNFPNGSQPPFILRFDASTLPVGQLVLSSPIRSNNELQDLANVY
                     VRASFTSVPGLVSPAPFGGNARSVVIKVDPALMREHNLTPDQIVAALRINNQPTPAGN
                     VRIGDLNYYTPANTTILNVKDFENIPIYTGGVQNIYLKDIARVEDGADITAGYALVNG
                     KRSVYLPITKAAEASTWEVVQNLKKAIPRFQAQLPEDVKLSYEFDQSTYVINAVKNLL
                     SEGAIGAILTGLMVLLFLGDVRGASIVIITIPICVISGVLFLHLCGQTINIMTLSGLS
                     LAVGILVDESTVTIENIHQHMAMGKPKSLAIWDACREIAFSKLLILFCILAVFAPALS
                     MTGIPG"
BASE COUNT          499 a          661 c          656 g          482 t
ORIGIN      
        1 ctgatcgcga cccaaaagct gatcgtttca cagcaaaaga acctggaccg ggcggtcgcc
       61 tttcaaacgg tggcaacggc ccgggcaaag acgggtctga atcccggtgt ggactcctcg
      121 cttgccaatg cggaggtctc caatgcccgg atcgcgctta ccaatgccat cgaggccgag
      181 caggaacagg ccaatgcgct ggggcagctc atgcaggtcc ccgtgcccga ctccaacaat
      241 tttgccctcg actcggcttt tatctcccgt ttacccgctt cgctgaacaa tgccccggcg
      301 cagccgctcc agaaccatcc cttgctgaag tattttaagg accgggtgaa ccttagccag
      361 gaacagtcca actatcttaa aacgcagcaa taccctacgt tgagcctgtt cggtgtcggg
      421 caggacaggg ggtcgggctt cgctactaac tatggcccgg gcaatccggg tgcgtatacc
      481 accagctatc tgaaaggggt cagcttcaac acggccaact atctgatcgg gatcggcgtc
      541 tactggaact ttacctcacc cttccgggta cgcgagcagg tggcggccca gagatggacc
      601 actgccgggc tgcaggaaga atatggcctg gtcagtcagg aactgcaggc ccagatggtc
      661 ctggcccaga gccgtatcgg gaatgccgtg aaaaattacc aggaggcgcc ggtgcaggta
      721 aaggcggcat cggatgccta tttacaaaag gagacgttgt acaagaacgg gctggccacc
      781 atcgtggatg taacgacggc cgcgttcatc ctcaaccagg cggaaacgca acgggatgtc
      841 atcgacacca atgtctggca ggcgctgttg tacaaggcgg catccaccgg ggatctcgga
      901 ttattcatca acgaatttta atcgcatcat atgggattga tcagacttgc gcttagaaaa
      961 ccgattacca tagtcgtgct ggtggcggga ttgttattct tcggcatcgg cgccgtccgg
     1021 agcatcaaga tcgatatctt tccgaagctg gatctcccgg tgatctatat ctctcatccg
     1081 tacggcgggt tcacacctga ccagatggaa tcgtatttcg gcaagcaata tgtgaacctg
     1141 ctgctctatg taggcggcgt aaagagcatc gagtcaagga acatacaggg gctcacgctg
     1201 atcaagctaa ccttttacga aggcaccaat atggcgcagg ccgcttcgga ggtcacgggc
     1261 tatatcaccc gtgcgcagtc caattttccg aatggctcgc agccgccgtt catcctgcgg
     1321 ttcgatgctt cgacgctgcc ggtgggtcag ctcgtgctga gcagcccgat ccgcagcaac
     1381 aacgagctgc aggacctggc caatgtctat gtgcgggcct ccttcaccag tgttccgggg
     1441 ctggtatcgc cggcgccatt tggcgggaat gcgcgaagcg tggtcatcaa ggtggatccg
     1501 gcgctcatgc gggagcacaa cctgacgccc gaccagatcg tggccgccct gcgtatcaac
     1561 aaccagccga cgccggcggg caatgtgcgg atcggggatc tcaactatta tacgcctgcc
     1621 aataccacta tccttaatgt aaaggatttc gaaaatattc ccatctatac cggtggcgtg
     1681 cagaacatct atctgaaaga catcgccagg gtcgaggatg gcgcggacat cactgccggc
     1741 tatgcgctgg tcaacgggaa gcgatcggtc tatctaccga tcacgaaagc tgccgaggcg
     1801 tcgacctggg aggtggtgca gaacctgaaa aaggcgatcc cgcggttcca ggcgcagctg
     1861 cctgaagacg tcaagctctc ctatgagttc gatcaatcca cctacgtgat caatgccgtg
     1921 aagaatctgc ttagcgaagg cgctatcggc gccatcctga cgggcctgat ggtgctgcta
     1981 ttcctgggtg acgtacgcgg cgcatccatc gtcatcatca cgatcccgat ctgcgtgatc
     2041 tccggggtgc tctttctaca cctttgcggc cagacgatca atattatgac gttaagcggg
     2101 ctgtcgctgg ctgtcgggat cctggtggac gagtcgacgg tgaccattga gaacatccac
     2161 cagcacatgg cgatggggaa gcccaaatcc ctggcgatct gggacgcctg ccgcgagatc
     2221 gctttttcca agctgctgat cctgttctgt atcctggcgg tctttgcccc ggcgctttcg
     2281 atgaccggca ttcccggc
//
LOCUS       DATGKE010000627         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1075278, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000627 DATGKE010000000
VERSION     DATGKE010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075278"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VKU83_10615"
     CDS             complement(<1..516)
                     /locus_tag="VKU83_10615"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94056.1"
                     /translation="MRHIVILLVTALVSAAGPACGGGKGPDAAKAGPGVEKFFPADDP
                     AIQYMGRIDSSDPKIPRFWAPGVVARFRFRGPSCRIILHDEVPNAYTHNYVVLLLDQG
                     SLGTYRVKLRGRTDTLTVWNNGRVRISEQDLKKEAQPGLPSQAPDPKRACMVTLCKAT
                     EGIAWMEMAGVL"
     gene            complement(561..1319)
                     /locus_tag="VKU83_10620"
     CDS             complement(561..1319)
                     /locus_tag="VKU83_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI94057.1"
                     /translation="MSSNSTTRFSDRVEDYVKYRPHYPPRILSYLRDVYSFHSDWAVA
                     DIGSGTGISTGMFLQNGNHVYAVEPNREMRAKAEELLREYPGFVSMDGTAEATGLEDA
                     SAGLIVAGQAFHWFDPERTRREFLRIGKPGVVVALMWNERLALSDFERDYEALILTYG
                     SDYTTINHKNVADEQIGAFFQPAPFLLQSFANEQRFDFAGLKGRLLSSSYIPKEGPRY
                     EEMIGALRALFDRYQDQGRVRVGYDTKLYTGRLV"
     gene            complement(1340..>2023)
                     /locus_tag="VKU83_10625"
     CDS             complement(1340..>2023)
                     /locus_tag="VKU83_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI94058.1"
                     /translation="MGKGSAVGKKLYYNWGGSSTVIGVVKDYVYGDMYGSPDPVIFYY
                     DSSNAALMYVRYKATVRPDVALATIGAVMKKDNPAYPFEYEFVDDDFNRLFAGEKLMG
                     QLSRLFAGLAILISCLGLFGLSAYTAERRTKEIGIRKVLGASVSGITQLLSREFLQLV
                     GLSAVIAFPVAWLAMGRWLEQYHYRISIQWWVFALAAAAAVVVALMTISFQSVKAALM
                     NPVRSLRSE"
BASE COUNT          448 a          663 c          490 g          422 t
ORIGIN      
        1 cagcacacct gccatttcca tccacgcgat tccttctgtt gccttgcata gcgtcaccat
       61 acaggcacgt ttcgggtcgg gcgcctgact gggaaggccg ggctgcgcct ccttcttcaa
      121 atcctgttcg ctgatccgca cccggccatt gttccacacc gtcagcgtgt cggtccggcc
      181 gcgcagcttc acccggtatg ttcccaggct tccctggtcg agcagcagta cgacataatt
      241 gtgcgtataa gcgttcggca cctcgtcgtg caggatgatc cggcagctcg gtcccctgaa
      301 acggaacctt gccaccactc ccggcgccca aaaccgcggg atctttggat ccgagctatc
      361 gatcctcccc atatactgga tggcagggtc atccgccgga aagaacttct ccacgccggg
      421 gccagcctta gcggcgtccg gcccctttcc accgccacat gcgggaccgg cagcggaaac
      481 cagggccgtc acgagtaaaa tcacgatgtg tctcatagat cttattgtgc aataaataca
      541 ccatgggcca tccccacgcc ttagacgagc ctgcccgtat aaagcttcgt gtcataaccc
      601 acccttaccc tgccctggtc ctgataccgg tcaaacaggg cccgaagggc cccgatcatt
      661 tcttcatacc gtggcccttc cttcggaata taggaagagg acagcagcct ccctttcaac
      721 ccggcaaaat caaagcgctg ctcgttggca aacgactgca ggaggaatgg cgccggctgg
      781 aaaaacgccc caatctgctc atcggcgaca tttttgtggt tgatcgtcgt gtagtcgctg
      841 ccataggtaa ggatcagggc ttcgtaatcc cgttcgaaat ccgatagcgc cagccgctcg
      901 ttccacatca gcgcaacgac cacgccgggc tttccgatcc gcaggaattc ccgccgcgta
      961 cgctcggggt cgaaccagtg aaaggcttgt cccgctacaa tcaggccggc gctggcgtcc
     1021 tccagcccgg tagcttccgc tgtgccgtcc atgctgacga aaccgggata ttccctcaat
     1081 aattcctccg cctttgctcg catctcacgg ttaggttcga ccgcgtacac atgattacca
     1141 ttttgcagga acatcccggt cgaaattccg gtgccggacc cgatatccgc gacagcccaa
     1201 tcagaatgga aagaataaac atcccgaaga tacgagagaa tgcgtggagg ataatgaggc
     1261 cggtatttta catagtcttc gacccggtcg ctgaatcgcg tagtgctgtt ggacgacatg
     1321 gctcgtgaaa atacggattt tattccgatc tgagacttct caccggattc atcagcgccg
     1381 ccttgaccga ctggaagctg atcgtcatga gcgcgacaac caccgcggcg gcggctgcga
     1441 gcgcaaacac ccaccactgg atcgaaatac ggtaatgata ttgctccaac caccggccca
     1501 ttgccaacca cgccacgggg aacgcgatca ctgccgataa cccgaccaat tgcaggaact
     1561 cccgcgacaa caactgtgtg atcccgctca cactcgcgcc cagcaccttc cggataccga
     1621 tctcctttgt ccgccgctct gccgtatacg ccgaaagccc gaacaaaccc aggcaagaga
     1681 tcaatatcgc cagcccggca aacaatctcg acaattgccc catgagcttc tcgccggcga
     1741 atagccggtt gaagtcgtcg tccacaaatt catattcgaa cggataggcc ggattgtctt
     1801 tcttcatcac tgcgccaatc gtcgccaacg ctacatccgg ccgcaccgtt gccttatacc
     1861 gcacatacat taaggctgca ttggagctgt cgtaatagaa tattaccggg tccgggctcc
     1921 catacatatc gccgtaaaca tagtccttta ccactccgat caccgtcgag gaaccgcccc
     1981 agttatagta tagcttcttg cccaccgcgc tgcccttccc cat
//
LOCUS       DATGKE010000628         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_970977, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000628 DATGKE010000000
VERSION     DATGKE010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970977"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VKU83_10630"
     CDS             <1..524
                     /locus_tag="VKU83_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI94059.1"
                     /translation="DNIRAVLEMTKLTRAGQKQKLEALLDEFHLHHVRKNNGDSLSGG
                     ERRRTEIARALAVDPKFILLDEPFAGVDPIAVEDIQSVVARLKFRNIGILITDHNVNE
                     TLSICDRAYLLIDGKIFKHGTAEDLAADEQVRRLYLGRNFELKRKDYLHEEARQHEMN
                     ESGAGDLLESDES"
     gene            597..2141
                     /locus_tag="VKU83_10635"
     CDS             597..2141
                     /locus_tag="VKU83_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLI94060.1"
                     /translation="MKLLSPAISRLARLRLGRIEEWIKNPIAVQREVLQHLVTSAQYT
                     EFGRKYDFSKTYTIRDFKRAVPIHDYEDLKPYIQRIMNGEENILWNTPITWFAKSSGT
                     TSDKSKYIPVSEESLQDCHYKGAKDVLTMYYDFNPDSDLLTGKGLVIGGSHTISQMNE
                     DVHIGDLSAVLLHNSPFWGHWIRTPELSIALLDEWEDKIERLAGSTIEENVTSISGVP
                     TWTLVLIRRILEITGKPSLSDVWPALELYIHGGVSFTPYIEQFKKLMGKDIHYLEMYN
                     ASEGFFAAQGNPQDDGMLLFLDHGVFMEFMPVEEYGKPEPETIGLADVEVGQQYALVI
                     NTNGGLWRYLLGDTVQFTSRYPFRIKVSGRLKHFMNAFGEEVIVDNTDKAIAVAAERT
                     GAIVNDYTAAPVYFGDTGNGAHEWLVEFEKEPDDMATFIRELDKALQSVNSDYEAKRH
                     KDIALSMPVLHSLRKGIFTAWLKSKGKLGGQHKVPRLSNNRKYIEEIQAMLPAPFALP
                     GSPLPK"
     gene            complement(2092..>2334)
                     /locus_tag="VKU83_10640"
     CDS             complement(2092..>2334)
                     /locus_tag="VKU83_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94061.1"
                     /translation="GTAVLHRLLVGLSFTVPLAFTVAAYRRKRNNIALAALKLSLVLF
                     YCFIDVPYLYLFYTSSLVSLVILAMAIREGQREPAT"
BASE COUNT          593 a          595 c          680 g          466 t
ORIGIN      
        1 aagataatat ccgggcggtg ctggaaatga cgaagctgac gcgcgccgga caaaaacaaa
       61 agctcgaggc cctgctggat gaattccacc tccatcacgt ccgcaagaac aacggggact
      121 cactgagcgg cggcgagcgg cgccggacgg agatcgcaag ggcattggcc gttgatccga
      181 aattcatcct gctcgacgag ccttttgccg gcgtagaccc gatcgccgtg gaagacattc
      241 agtccgttgt tgcccggctg aagttcagga atatcggcat tctgatcacc gaccataatg
      301 tcaacgagac cttgtcgatc tgcgaccgcg cgtatctgct gattgatggg aagatcttca
      361 aacatgggac ggccgaggat ctggcggcag atgagcaggt gcggcggctg tacctgggga
      421 ggaatttcga actgaagcga aaggattacc tgcatgagga ggccaggcag catgaaatga
      481 atgagtcggg cgcgggagac cttttggaat ccgatgagtc ttaacgactt acttcgtcaa
      541 aagcgccgct gcgtatttgt ttgagtattt cccggcgatt aactattttg ccagccatga
      601 agttgttgag cccggctata tcgagactgg ccagactgcg tctggggcgt attgaggagt
      661 ggataaaaaa cccgattgcc gtacaacgcg aggtgttgca acacctggtg acgtcggcac
      721 aatatacaga gttcggaagg aaatacgact tttcaaaaac ctatacgatc agggatttca
      781 agcgggcggt ccccatccat gattatgagg atctgaagcc ctatattcag cgcatcatga
      841 atggggaaga gaacattctg tggaacacgc ccatcacctg gtttgccaag agcagcggga
      901 cgaccagcga caagagcaaa tacattcccg tcagcgagga aagccttcag gactgtcact
      961 ataaaggggc gaaggacgtg ctgacgatgt attacgactt caatccggac tccgatctgc
     1021 tgaccggtaa aggcctcgtg atcggcggca gccatacgat cagccagatg aacgaggacg
     1081 tgcatatcgg cgatctcagc gccgttttat tgcataactc gcccttctgg ggccactgga
     1141 tcaggacgcc cgagctcagc atcgcgctgc tggacgagtg ggaagacaag atagaaaggc
     1201 tggcgggcag taccatcgaa gagaacgtca cgtcgatttc aggggtccct acctggacgc
     1261 tggtgctcat ccgccggatc ctcgaaatca cgggcaagcc cagcctctcg gacgtctggc
     1321 ctgccctgga actttatatc catggcggcg tttcctttac gccatacatt gagcagttca
     1381 aaaagctgat gggcaaggac attcattacc tggagatgta caatgcaagc gaaggcttct
     1441 ttgctgcaca gggcaatcct caagacgacg gcatgttact cttcctcgat catggcgtct
     1501 ttatggaatt catgccggtg gaagaatacg gcaagccgga gccggagacc atcggactgg
     1561 cggatgtcga agtgggccag cagtatgccc tggtcatcaa cacgaatggc ggcctgtggc
     1621 ggtacctgct cggagacacg gttcaattta cttcccggta tccattccgg ataaaagtca
     1681 gcggccggct gaagcatttt atgaacgcct ttggggaaga ggtgatcgtc gacaatacgg
     1741 ataaggccat cgcggttgcg gccgaacgga ccggcgccat tgtcaacgat tataccgccg
     1801 cgccggtgta tttcggtgac acgggtaacg gcgcgcacga gtggctggtg gaattcgaaa
     1861 aggagccgga tgacatggcc acatttatcc gcgagctgga taaggcctta caaagcgtga
     1921 acagcgacta tgaagcaaaa aggcataagg acatcgcctt gagcatgccg gtgctgcact
     1981 ccctcaggaa gggcattttt accgcctggc tgaagagcaa gggaaaactg ggcgggcagc
     2041 acaaggtccc gcgcctgtcc aacaatcgga aatacatcga ggagatccag gctatgttgc
     2101 cggctccctt tgcccttccc ggatcgccat tgccaaaatg acgaggctta ccaatgaaga
     2161 ggtatagaaa agatagaggt aaggcacgtc aataaaacaa taaaataaga cgagacttag
     2221 cttaagcgcg gcgagcgcga tattgttcct tttcctccgg tacgcggcca cggtaaaagc
     2281 aagcggcacc gtgaaactca ggcccacgag gaggcggtgc aacaccgcgg tgcc
//
LOCUS       DATGKE010000629         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_621659, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000629 DATGKE010000000
VERSION     DATGKE010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621659"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VKU83_10645"
     CDS             <1..681
                     /locus_tag="VKU83_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94062.1"
                     /translation="DLYDSKNAVISDCVINSGDDAIALISSSNIVVDNCILHSRSSGI
                     RIGYNVFNHRNSGDLLFNNIRIFDSNRGIGIFQRMDGDMSNLIFSNLIIQTRLHTGEW
                     WGHGEPIHISTVPGLGSKTTGQIRNVRFSHVVATAQTGIVLYSSRPGLLKDITFEDVD
                     LTIERGPLSESYGGNIDLRPVNDLSLAIFRHSIPAVFAYQVEGLDIRHFHTHWGAGLP
                     SYFLKGKN"
     gene            791..982
                     /locus_tag="VKU83_10650"
     CDS             791..982
                     /locus_tag="VKU83_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94063.1"
                     /translation="MKHITKKVVGLALFLGVIALALPGCYEGHYYNTYHHHTHGWYDR
                     RHQPPPPNVRWDVDVDVRN"
     gene            1107..1730
                     /locus_tag="VKU83_10655"
     CDS             1107..1730
                     /locus_tag="VKU83_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94064.1"
                     /translation="MSYLHQMAKKWRDPLRIFKLFQCAIAVICVSIPALLRLADKNSP
                     GFRNSISQYVYISDSYIYGMLLCVAAMMFIFNGAVYFQNEERLGLKKAGKWYNVVLGL
                     SLFGVILFPPHTFEVVHYLFGGLFFIGNALVTGFFHNKKDRVISITLAVLTLLGFVFC
                     YIVPWISLLAAEWISLAVISLHFIMQSGFSFRLDPGSTQRMAPERIR"
     gene            1877..>2868
                     /locus_tag="VKU83_10660"
     CDS             1877..>2868
                     /locus_tag="VKU83_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLI94065.1"
                     /translation="MKKNCCLAGIMVFLFCMHLRALAIGDPQVKHLGIEQGLSNNAVT
                     CIYQDAKGFLWFGTYDGLNRYDGYGFTVYRNIIGDSASIPFNNVASINADQHDHIWVS
                     GLKGIAILDPLTGKFAIPAYRSCDGHGIVKVQSFVHMIRSVGDRYVLAGSHSNGLLLF
                     DGQSSVGWQIPLKELRGNQGSYDVFAVEYDKFTGRVWVLIQGKGLYALDLATKTLSPV
                     NTTFRNGYSLKVDNNGTLWMGTDNGLFALSRDGRTFSANYYLPCKSRVVCIDVNSRND
                     LCFGTDGDGIWCLPANAAKAMPMLAATGASLVNSNAVYSVYEDREGRKWIGTLRG"
BASE COUNT          637 a          800 c          739 g          692 t
ORIGIN      
        1 gatctctacg attccaaaaa cgccgtcata tcggattgtg tgatcaattc aggagacgat
       61 gcgatagcgc tgatcagcag cagcaatata gttgtcgaca attgcatcct gcattcccgt
      121 tccagcggga tccgcatcgg ctacaacgtc ttcaatcacc gcaactccgg cgatctgctg
      181 ttcaacaata tccgcatctt cgactccaac agggggatcg gcatctttca gcgaatggac
      241 ggagacatgt cgaacctgat attttccaat ctgatcattc aaacccggct gcacacggga
      301 gaatggtggg gtcacggcga gccgatccat atttccaccg tgcccggtct gggcagcaaa
      361 acgacaggcc agatccgaaa tgtccggttc tcacacgtcg tggccaccgc tcagaccggt
      421 atcgtcctgt acagttcgcg tcccggtctg ctgaaagaca taaccttcga ggacgtcgat
      481 ctgaccatcg aacgcggccc cttatccgaa agctatggcg ggaacatcga tctgcggccg
      541 gtgaacgatc tttcgctagc catctttcgc cattcgatcc ccgccgtgtt cgcttatcag
      601 gtagaaggtt tggacatcag gcatttccac acccattggg gcgccggtct tccgtcctac
      661 ttcctgaaag ggaaaaatta atgcaggcgc cgtccaacct gttccattca tttaaaattg
      721 acaatcaacg tattgccagg ccggcagcgc atggtaggag atttgcatcg atttaagtaa
      781 aatcgatttt atgaagcaca tcacaaagaa agtagtaggc cttgccctat tcctgggtgt
      841 catagcgctc gccttgccgg gctgctacga aggccattac tataacacct accatcatca
      901 tacacatgga tggtacgatc gccgccacca accgccgccg cccaacgtgc gctgggatgt
      961 cgacgtcgac gtccggaact agcgctaagg acacgataac ccatttatta taccgacccg
     1021 ggatgccccg ggtcattttt ttgggtgttt tcacggttgt atctgtgcac cttctttatt
     1081 aagtttagtt aaaacaagga ggtgttatga gctatctaca ccaaatggcg aagaagtgga
     1141 gagatccgct aaggattttc aagctcttcc aatgcgccat agccgttatt tgcgtgagca
     1201 ttccggcact gctccggctg gctgataaaa attctcccgg tttccgcaat agcatcagcc
     1261 agtacgtata catcagcgac agctatatct atggaatgct gttgtgtgtg gcagcgatga
     1321 tgttcatctt caacggagcg gtctattttc agaatgaaga gcgcctcggt ttaaaaaagg
     1381 ccggcaagtg gtacaatgtg gtcctgggtc taagtttgtt cggcgtgatc ctgttcccgc
     1441 cccatacgtt cgaggtagtg cactatcttt ttggcggact tttctttatc ggcaatgcgc
     1501 tggtaaccgg cttctttcac aacaaaaagg atcgcgtgat cagcatcacc cttgccgtgc
     1561 tgaccctgct tgggttcgta ttctgctata tcgtaccctg gatctcgctg ctggcggcag
     1621 agtggatatc gcttgccgtc atttcactgc actttatcat gcaatcgggg tttagcttcc
     1681 ggcttgaccc cgggagcacg caacgcatgg cgcccgaacg gatccgatag aggttgcccc
     1741 cgtattgctc cgaccgcagt tgttgtaccg gattggcccg caccgccttt ttcggcacag
     1801 catctgcgct gttttcgcta aatttaccgt gaatagggcg caatggcgtc cgctgctaca
     1861 caaacgccgc tgctatatga agaaaaactg ctgcttagcc gggataatgg tctttctatt
     1921 ctgcatgcat ctgcgtgccc tcgccatcgg tgatccgcag gtaaaacacc tcggtataga
     1981 acagggcctg tccaacaatg cggttacctg catctaccag gatgccaaag gtttcctgtg
     2041 gttcggcacc tatgacggat tgaaccgtta cgacggttac ggctttaccg tttaccgaaa
     2101 tatcatcggt gactccgcct ccatcccctt caacaatgtc gccagcatca acgccgatca
     2161 gcacgaccat atctgggtaa gcgggttaaa aggtatcgct attctcgacc cgctcaccgg
     2221 taagttcgcg ataccggcct accggtcctg cgacgggcac gggatagtga aagtgcagag
     2281 cttcgtgcac atgatccggt cggtgggcga ccgctatgtg ttggccggtt cgcattccaa
     2341 cggcttgctg ctcttcgacg ggcaatcgtc cgtcggctgg cagatcccgc tgaaggaact
     2401 ccgcggcaat cagggaagct atgatgtatt cgccgtagaa tacgacaaat tcacgggccg
     2461 ggtgtgggtc ctcattcagg gaaaaggatt gtatgccctc gacctggcta ccaaaacgct
     2521 ctcgcccgtg aacaccactt tccggaacgg ctattcactg aaggtcgaca acaacgggac
     2581 gctctggatg ggcaccgata acggcctctt tgcgctgtcg agggacgggc gtactttctc
     2641 ggccaactac tacctgccct gcaaaagccg cgtcgtgtgc atcgatgtca atagccgcaa
     2701 cgatctttgt ttcggcaccg acggagacgg gatctggtgc ctgccggcca atgccgcgaa
     2761 ggctatgccg atgctggccg ctaccggcgc ctccctggtc aatagcaatg cggtatactc
     2821 cgtttatgaa gaccgcgaag gccgtaaatg gatcggcacg ttgcgcgg
//
LOCUS       DATGKE010000630         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_150401, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000630 DATGKE010000000
VERSION     DATGKE010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150401"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VKU83_10665"
     CDS             complement(<1..592)
                     /locus_tag="VKU83_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94066.1"
                     /translation="MKIAVVILILIWSSALKAQSIDRIVTTAAVNETESVLASDAMRG
                     RGSLTPDIDRAADYIEARFHKAGLKTWNGSSSYRQPFALLRTTPLSSEATIDGAPLPA
                     NHVIAITSSTDLAVDENSGYKKVYIRSGGDFRAAARSYLHEKENLVVFVDTAYTASFP
                     MLMRSARQGFKSDRSVIFILTNADPAHYTLHVRQDVK"
     gene            complement(604..1338)
                     /locus_tag="VKU83_10670"
     CDS             complement(604..1338)
                     /locus_tag="VKU83_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HLI94067.1"
                     /translation="MIRAIIIDDERLARNELKKLLLDFPEIEVIAEAANAAEGVEKID
                     SLNPDLIFLDIQMPGKTGFDMLSELERAPNVIFTTAYDEYALKAFEVNALDYLLKPVE
                     PKRLADALQKLQVEEDKEPISDHMISVNRSILNEHDQVFVKDGERCWFVKLSDIRLFE
                     SVGNYAKVYFGPNKPLILKSLNALEERLDEKVFFRANRKHIVNLRLIEKIEPYFNGGL
                     LLEMKGGEKIEVSRRQTVKFKEMMSL"
     gene            complement(1363..>2314)
                     /locus_tag="VKU83_10675"
     CDS             complement(1363..>2314)
                     /locus_tag="VKU83_10675"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLI94068.1"
                     /translation="KITDKQIEIMGIIVLTGILVTHVFRGIIKRSGWALLPVDKALPK
                     FIIGVVLSCICIALIRIGVVDALKLVDHKGAEFMPRLLTGSADAGLLIIPWTLIYYFY
                     HYIEITKKQEVDTLKLEALVKELELKTIKAHINPHFIFNALNSIRALIDENPARARTA
                     ITELSNILRSSMQAEKLETVTFEKELNIVKDYLALEHIRFEDRLQVEYEIDEDTLDQP
                     VPPMMLQTLVENAIKHGISRQVDGGKVRIVSDFREDYHELVILNTGVLNGSRDSDGFG
                     LASTKNRLQLLFGQKANFDIREVNANTVQARVLIPVHTTR"
BASE COUNT          508 a          579 c          642 g          585 t
ORIGIN      
        1 cttttacgtc ctgccggaca tgcaacgtat aatgtgcggg gtctgcattg gtgaggataa
       61 aaatgacact gcgatccgat ttgaagccct gccgggcact gcgcatgagc atgggaaaag
      121 aagcggtata agcggtatcg acaaagacca ctaaattctc tttctcgtga aggtaggacc
      181 tggccgcggc tctgaagtcg cccccggacc ggatataaac ctttttatat ccgctattct
      241 catccacggc gagatcggtc gaggaagtaa tggctatgac atgattggcc ggaagggggg
      301 cgccgtcgat cgtcgcctcg gaggaaagcg gagttgtgcg aagcagggca aaaggttgac
      361 ggtagctgga gctgccgttc caggttttca gaccggcctt gtggaaacgc gcttcgatat
      421 aatcggccgc ccggtctata tcgggtgtaa ggcttccccg accacgcatg gcatccgagg
      481 ccagtacgct ctcggtctcg ttcacggcgg cggtagtaac gatccggtcg atggattgtg
      541 ccttaagggc agaggaccag atcaggatca gaataacaac ggctatcttc atcgattcgg
      601 gatttaaagg ctcatcatct ctttgaactt gaccgtctga cgacggctga cctctatctt
      661 ttccccgcct ttcatctcca gtagaagtcc gccgttgaaa taaggttcga tcttttcgat
      721 cagtcggaga ttcacgatat gcttgcggtt agcccggaaa aagacctttt cgtccagtct
      781 ttcttccagg gcattcagcg atttcaatat caggggtttg ttcggaccga aatacacttt
      841 ggcataattg ccgacgctct cgaagagccg gatgtccgat agcttgacaa accagcagcg
      901 ttcgccgtcc tttacaaaga cctgatcgtg ttcgttgagg atgctgcggt tgacggagat
      961 catatggtcg gagatcggct ctttgtcctc ttccacctgg agtttttgca gggcgtcggc
     1021 cagacgtttg ggctccacag gtttcagcag atagtcgagg gcattcactt cgaaggcttt
     1081 caacgcatat tcgtcataag cggtggtaaa gatgacattg ggagcccgtt ccagctcgga
     1141 gagcatgtcg aaacccgttt tacccggcat ttgtatatcc aggaagatga gatcaggatt
     1201 gaggctgtcg atcttttcga cgccttcggc tgcgttggct gcttcggcga tgacctcgat
     1261 ctcggggaaa tccagcagca gtttcttgag ctcgttcctt gctaaacgtt cgtcgtcgat
     1321 aatgatggct ctgatcatat gtaatggggc cttggggccg gtttatctgg tggtatggac
     1381 ggggatcaat accctggctt gcacggtatt ggcattgacc tcccggatgt caaaatttgc
     1441 cttctggcca aaaagaagct gtagcctgtt cttcgtacta gccaggccga agccgtcgga
     1501 gtcacggctt ccattgagaa cgccggtatt caggatcacg agctcgtgat aatcttctct
     1561 gaaatcggag acgatcctaa ccttcccacc gtcgacctgg cggctgatcc catgtttgat
     1621 ggcattttcg acgagcgtct gtaacatcat cggcggcaca ggctgatcca gcgtatcttc
     1681 gtcaatctcg tattccacct ggagacggtc ctcaaaacgg atatgttcca gggcgagata
     1741 gtcttttacg atatttagct ctttttcgaa ggtcacggtc tccagctttt ccgcttgcat
     1801 ggagctgcgg aggatattgc tgagctcggt gatggcggtg cgggcgcggg cggggttctc
     1861 atcgatgagg gcgcggatgc tgttgagggc gttgaagatg aaatgcgggt tgatatgcgc
     1921 tttgatggtt ttcagctcca gctctttgac cagggcctcc agtttcaacg tgtcgacctc
     1981 ctgcttcttg gtgatctcga tgtaatgata gaaataatag atgagggtcc acggaatgat
     2041 caacagacca gcatcggccg atccggtgag cagacggggc atgaactcag cccccttatg
     2101 atccaccagc tttaaagcat ctaccacgcc gatccggata agggcaatgc agatacagga
     2161 caggaccaca ccgattatga atttcggcaa ggctttatcg accggcagga gcgcccagcc
     2221 ggaccgttta atgatcccgc gaaaaacatg ggtcaccagg atgcctgtga gcactataat
     2281 acccatgatc tcgatctgct tgtccgtgat cttc
//
LOCUS       DATGKE010000631         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_371238, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000631 DATGKE010000000
VERSION     DATGKE010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371238"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2710)
                     /locus_tag="VKU83_10680"
     CDS             complement(<1..>2710)
                     /locus_tag="VKU83_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94069.1"
                     /translation="STHLGPAIGKELPSRYGPNHPLFYLYPFLVIVLPNIFFTSALFH
                     GLVAVLRNVKVIYFGGVVLFLFYFMALFFLNNTTNARVIAIADPFALNGVRAQMMSAN
                     YIQQNTSLLLFRGPLVVNRILWPGLSLLVVLVTYFRFNFETFFAGRGDRARPDEPGAR
                     SQTALQKPTLSFTGDYNRRTLASLVRLELLNIIRDNYFWVIIGVGSFFLGFVFWLGMQ
                     NYGVHDAPRTVMMLAIFDDAFPFLIFFVIMFYTGETLHRDRITRYSFINDSLPPPNWV
                     LNGSKLIPILILSTVLAFMPVVVGIGVQLLKGYPHLNLAAWSDAVFYLVLPKFLAGAV
                     LCYLVQVLFNNKFVGYAIVVPLWVGMFFLDSTGNYDYHLLLYSFTPNTGISDMDGIGH
                     MKGPISWFDVYWMLCAGLLVIVAALLYNRGLNSSLKERWQLVPERFNRATRRWTIGLA
                     ALFLGVGAYIYYNVSYVNEWLTKGEQRERAVMYERSLKKYQKLPLPKMVQLTEWVDLF
                     PGEQAQRVHALITLVNRNRQPIKEMLLDGDALTGYSISLQGKPLPFSSPLRYPRGAYS
                     WFRPAWDTAPFRLYEFPAPLAPGDSMVLDLRSVVEHKGFQNGFFAGNMLDNGTIFTGG
                     LPGLGYDDDDELSSPYERQEAGLPPKADEEIAQDDPVERNNLRSGAGEDLVRLDLTVS
                     VPGDQTALSPGNLVRQWQSGGRNYFHYMLDKPGLYGPFVLVSARYAVSRASVMLDHDV
                     DISIYYDPRHGENLGRFMAAYKEGLAYFSKAYGDYPFRHFVLAESSNYGPRTMSLESM
                     NAVGEPGYWNAHFTDPGQPDFIYHLASYMLAQQWWRYRVAPNNTAGSLVISEGLANYD
                     ALVMDEKHAGAAGVRPFLDNEIFIHNLIRRRETDPEKPLVKAKYGWM"
BASE COUNT          659 a          772 c          747 g          532 t
ORIGIN      
        1 catccacccg tatttggcct tcaccagcgg tttttccggg tcggtctcgc gacgccggat
       61 caaattgtgt ataaagatct cattgtccag gaaaggcctt acgccagcgg ctccagcatg
      121 cttctcgtcc atgacgagcg catcatagtt ggccaggccc tcggagatga ccagggagcc
      181 cgccgtattg ttcggcgcca cccggtaacg ccaccattgc tgggccagca tataggaagc
      241 cagatgatag atgaaatcgg gttgtccggg gtccgtaaaa tgcgcgttcc agtatcccgg
      301 ctccccaacg gcgttcatgg actccaatga catcgtgcgg ggcccgtagt tggatgactc
      361 ggccagcaca aaatgccgga agggatagtc accgtaggct ttgctgaaat aggccagccc
      421 ctccttatag gcggccatga accggccgag attctctccg tgccgcggat cgtaatagat
      481 cgagatatct acgtcgtgat ccagcatcac gctggcccgg ctgaccgcat atcgcgccga
      541 taccaggaca aagggcccat acaggccggg tttgtccagc atatagtgga agtaattccg
      601 gccgccggat tgccattgcc ggaccaggtt cccgggactg agtgcggttt gatcgccggg
      661 aacgctgacg gtgagatcca gccggacgag gtcctcgccg gcgccggacc ttaaattatt
      721 cctttcaacg gggtcgtcct gggcaatttc ttcgtcggct ttcggtggca agccggcttc
      781 ctgccgctca tacgggctcg atagctcatc gtcatcgtca taacccaatc ccggcaggcc
      841 tccggtgaaa atggttccgt tgtccagcat gttgccggca aaaaaaccgt tttgaaagcc
      901 tttgtgctcc accaccgaac gaagatccag gaccattgaa tcacccggcg ccaaaggcgc
      961 tggaaattcg tagagccgga aaggggcggt gtcccaggca ggccggaacc agctgtacgc
     1021 accgcgtgga tagcgcaggg gagaactaaa aggcagcggc ttgccctgga gtgaaatgga
     1081 ataaccggtc agcgcatcgc catccagcaa catttccttt atcggctgcc ggttccgatt
     1141 gacaagcgtg atcagggcat gtacccgttg cgcttgttct cccggaaaca aatcgaccca
     1201 ttcggtgagc tgcaccattt ttggcaacgg cagcttttga tattttttca gtgaccgctc
     1261 atacatcacc gcccgttcgc gctgttcgcc cttcgtcagc cactcgttca catagctgac
     1321 gttgtagtag atgtaggcgc cgacgccgag aaacagtgcg gccagtccga tcgtccacct
     1381 ccttgtggcc cggttgaagc gttcgggcac cagttgccag cgttccttga gcgaggaatt
     1441 caatccccgg ttatacagca gcgcggcaac gatcaccagc aacccggcgc aaagcatcca
     1501 atagacatcg aaccaactga tcgggccttt catgtgccct attccgtcca tatccgaaat
     1561 gccggtattc ggtgtaaagg aatacaataa cagatgataa tcgtaattgc ccgtggaatc
     1621 gaggaaaaac atcccaaccc atagcggaac gacgatagca tagccgacaa acttattgtt
     1681 aaaaagcacc tgaaccagat agcacaggac tgcccccgcc aggaacttcg gcaacaccaa
     1741 atagaaaacc gcgtcggacc aggcggcaag attcaggtgc ggatagcctt tcaacaactg
     1801 cacacctatt ccgaccacca ccggcataaa ggccagcacc gtactcagga taaggatagg
     1861 gatcagcttg gagccgttca acacccaatt gggcggcggc agcgaatcgt tgataaagga
     1921 atagcgggtg atccggtccc gatggagtgt ctccccggtg tagaacatga taacgaaaaa
     1981 aataaggaat gggaatgcat cgtcaaagat cgcgagcatc atcactgttc gcggcgcgtc
     2041 gtggaccccg tagttctgca tccccaacca gaagacaaaa ccgaggaaga agctgcctac
     2101 gccgatgatc acccagaaat agttatcccg gataatattg agcagttcga ggcggaccag
     2161 gcttgccaac gtccgccggt tataatcgcc cgtgaagctg agggtgggct tttgcaaggc
     2221 agtttgcgat ctcgcgcctg gctcgtcggg cctggcccgg tccccccgcc cggcaaagaa
     2281 cgtttcgaaa ttgaaccgga aataggttac cagcacgacc agcagcgaca gtcccggcca
     2341 caatatccgg ttgacgacca gcggccccct gaaaagcagc agactggtat tctgctggat
     2401 atagttcgcg ctcatcattt gcgcccggac gccgttcagg gcaaaaggat ccgcgatggc
     2461 gatcacgcgg gcgtttgtgg tgttattgag aaaaaacagc gccatgaaat agaaaaggaa
     2521 gaggaccacg ccgccgaaat agatcacctt cacgttccgc agcaccgcca ccaggccatg
     2581 gaaaagcgcg gaagtaaaga agatgttcgg cagcacgatc accaaaaatg ggtagaggta
     2641 gaacagcgga tgattgggcc cgtaccggga gggcagctct ttcccgattg ccgggccgag
     2701 atgtgtcgat
//
LOCUS       DATGKE010000632         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_142227, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000632 DATGKE010000000
VERSION     DATGKE010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142227"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VKU83_10685"
     CDS             <1..1019
                     /locus_tag="VKU83_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI94070.1"
                     /translation="GLLFFVPLGDKVERRGQIVWMAAAAVGALVLAACSPNLLTMEIA
                     SVLIGATCVIPQLVLPMAAHLASPARTGKVIGTVMSGLLVGILLSRTLSGFVGAWLGW
                     RGMFWVAAGITFSLLLTIRFFFPLSRPAFAGSYGSLMRSLLTLTREQPVLREAAFINA
                     LGFSTFGMFWTTMVLHLSGPPFHFGSGVIGLFGLAATAGALAAPLVGGAADKRNPRIT
                     IGYGLGMLFISWVIFYTQAGTVAGMVAGIVLLDLAMQCIHVSNQSRVYALMPAARNRM
                     NTVYMTVSFAGTSLGSAIGLFAWNEGAWNGVCLSGVVLTLAAFSIFAGTARRAKKTAR
                     AALR"
     gene            1121..1429
                     /locus_tag="VKU83_10690"
     CDS             1121..1429
                     /locus_tag="VKU83_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI94071.1"
                     /translation="MNIYISNLSFNVTDDDLQDYFAEYGEVNSAKVIMDKFTGKSRGF
                     AFVEMSDDEAAKKAIQELDGATVDGRTIGVSVAKPREDRAPGKGGSFQRRNNYSSSRY
                     "
     gene            complement(1488..1889)
                     /locus_tag="VKU83_10695"
     CDS             complement(1488..1889)
                     /locus_tag="VKU83_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI94072.1"
                     /translation="MATDLYTPILLHRFVDELMAGLLPRAVSRKTIIINQIDRDLELG
                     TDENLLAFILWNLLDRAVACTQNECIHVESAREGENTMIRVRNAGVYFYRTLANNFRQ
                     VQHAAEKLGGTISIDYTEDTCTTVALTLRAA"
BASE COUNT          395 a          541 c          645 g          518 t
ORIGIN      
        1 tgggcctgct gttcttcgtc ccgctcggcg acaaggtgga gcggcgtggg caaatcgtct
       61 ggatggccgc cgcggccgtc ggggcgctgg tcctggcggc ctgttcaccc aacctgctga
      121 cgatggagat cgcgagtgtg ctgatcgggg ctacctgcgt tatcccgcaa ctggttttgc
      181 cgatggccgc gcacctcgcg tcgccggcgc ggacgggcaa agtgatcggg accgtaatga
      241 gcgggcttct ggtcggcatc ctgttgtccc gcacccttag tggatttgtc ggcgcttggc
      301 tgggctggcg gggaatgttc tgggtggcgg ccggcattac gttttcgctt ttgctcacca
      361 tccgcttctt ctttccgctg agcaggccgg cgtttgctgg atcttatgga tcactgatgc
      421 ggtcgttgct gacgctgacg cgggagcagc cggtgcttcg cgaagcggcg ttcatcaatg
      481 ccctgggctt ctcgactttc gggatgtttt ggaccaccat ggtgttgcat ctttcaggcc
      541 ccccttttca tttcgggagc ggggtgatcg ggttgttcgg actcgccgcg acggcggggg
      601 cgctggcggc gccattggtc ggcggcgccg cggacaaacg caacccgcgc atcacgatcg
      661 gctatggatt ggggatgctt ttcatctcgt gggtgatctt ttatacccag gcgggaacgg
      721 tcgccggcat ggtggccggg attgtgctcc tggacctggc gatgcaatgc attcatgtct
      781 cgaatcaaag ccgggtatat gccctgatgc cggccgcgcg gaaccggatg aacacggtat
      841 atatgaccgt tagtttcgcc ggcacctccc tcgggtccgc catcgggctt ttcgcgtgga
      901 acgagggcgc gtggaacggc gtctgcttgt cgggggtggt gctgacgctg gcagcattca
      961 gtattttcgc cgggacggcc aggagagcta aaaaaacggc tcgcgcggcc ctccggtgat
     1021 ggcgcgccgc ggtccgggcc gataaatccg tttgtgcatt gccaattgag gaaaaggtcg
     1081 taactttgcg gcgacttttt tttaaccagt taatttcagc atgaacattt acatttcgaa
     1141 cttgagtttc aacgtcacgg atgacgactt gcaggattat tttgcagaat acggcgaagt
     1201 caattctgcc aaggtcatca tggacaaatt caccggcaaa tcccgcgggt ttgcttttgt
     1261 ggaaatgtcg gatgacgaag cagcaaaaaa agctatccag gaactggatg gcgctaccgt
     1321 cgatggcagg accatcggcg tatccgttgc caaaccgcgg gaagaccgcg cccccggcaa
     1381 aggtggaagt ttccagcgca ggaacaatta ttccagcagc agatactaga aaggcatttt
     1441 gctcggcgaa gggatcccgc aggggtccct ttttttatgc gccgggatca ggcggcccgg
     1501 agcgtcagcg ccaccgtagt gcaggtgtct tcggtatagt cgatgctgat ggttccgccc
     1561 agtttctccg cggcatgttg gacctgccga aaattattgg cgagggtccg gtagaaatag
     1621 accccggcat tccggacacg gatcattgta ttctcgcctt cccgggcgga ttccacgtgg
     1681 atgcattcgt tctgcgtgca ggcgacggcg cgatccagca aattccacag gatgaaagcc
     1741 agcaggttct catccgtgcc cagttcgagg tcccggtcaa tttgattgat gatgatcgtt
     1801 ttgcggctta cagctcgcgg aagaagtcca gccattaatt cgtccacaaa acggtgcagt
     1861 aatatgggag tgtacaaatc agtagccatt gatacaggat tttagtaatg ttgcttttaa
     1921 tcgcttaaag gtaacgcaac cggacattag tttatgtaga gcgttgcgga aatttttaca
     1981 aaattgctat tttcagccta tgaattttat aggaaacctt atatggttga tctgcggtgg
     2041 cttctttgcc gcagtgggat acatcgtcgg cggcttcgtt ctttgtctga ctgtgatcg
//
LOCUS       DATGKE010000633         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1177505, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000633 DATGKE010000000
VERSION     DATGKE010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177505"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VKU83_10700"
     CDS             complement(<1..1907)
                     /locus_tag="VKU83_10700"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HLI94073.1"
                     /translation="MRRRSFIKRGSLLAGGYTLGAAVPTAVARYWPLSEDRLYKLFRN
                     PAMASRPFARWWWNGDKIEKSELARELRLLKDAGFGGVEINPIAFPERTADMGIRSVD
                     WLSPAWLELLRFTLEEAGTLGLTCDLIVGSGWPFGAPWLTGEERSRVRVISAVKLNGP
                     ADYPALVADLIKDAYPAISAPFAGRVSKLISLSLVPTPLNNLEEVKEIPVEPGRMTYT
                     IAVPSGSHMLYALVEFDGFSEVINGAPGANGPVLNHYNKAAVEKYLHRMSDALGPLTS
                     HIRCFFTDSLELEGANWCDDMEKEFLSRRGYELKPYLPFILYKTGRMGNDIDPEYGAK
                     HGEVFRQLTGRVRFDFDRTKTELLRERFVEPFLAWCKENRVLSRMQAYGRGYDPLDGS
                     FGVDIPECETWIKAGLGTQMSEDDYRLGRAYTMINKYISSAAHLQDKNIVSCEELTNT
                     DGVFNETLEILKVGSDQSIISGVTQAVFHGFNYSPPEAAFPGWVRYGTFFNERNPWWP
                     WLPHFTAYRARLTAVFHEMTMDAGIALLPPIADTWNRYGAQNTPFPTLIHPTYQPLIW
                     EAIHKNGDGCDYVSERTIQAGLLQKYHTLFLIEVESLSPATAEMLYRFAAGGGRIFCI
                     GSIPSKAEGLLT"
     gene            complement(1973..>2558)
                     /locus_tag="VKU83_10705"
     CDS             complement(1973..>2558)
                     /locus_tag="VKU83_10705"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLI94074.1"
                     /translation="KELMLDERIGRMAAGLAGVEGIRIWHDQALFKRPWANPTAWHLD
                     TPFWSFSDRRAISIWVALDDATLQNGCLYFIPGSHKGTNFDKITIGRNMDSIFDVYPK
                     FGKTPAVAAPMRAGSCSFHNGLTIHGAGANMTNGWRRAMTCAYMPDGNVFNGEPNILP
                     EPYLSSLKPGDLLNNNEQNPLIYSAIARALRGFS"
BASE COUNT          552 a          746 c          676 g          584 t
ORIGIN      
        1 gtcagcaaac cctccgcctt tgatgggatg gatccaatgc agaagatcct tccgcccccc
       61 gctgcaaaac gatacagcat ctccgccgtg gcggggctca ggctctccac ttcgatcagg
      121 aacagcgtgt gatatttttg taaaaggccc gcctggatcg ttctttccga aacataatca
      181 catccgtctc catttttgtg gatcgcttcc caaatcaatg gttgataggt gggatggatc
      241 agcgtcggaa atggagtatt ctgtgcgccg tatctgttcc aggtatcggc gataggggga
      301 agcaaagcga taccggcatc catggtcatt tcgtggaaga ccgcggtcag ccttgcccga
      361 taggcggtaa aatgcggcag ccacggccac cagggattcc gttcattaaa aaaagtgcca
      421 tagcggaccc agcccgggaa cgcggcctcc ggcggcgagt aattgaaccc gtgaaaaacc
      481 gcctgtgtga cgcctgaaat gatgctttga tccgatccca ccttgagaat ttccaaagtc
      541 tcgttgaaaa ccccgtcggt attggtcaat tcctcgcagg aaacgatgtt tttatcttgt
      601 aaatgcgcag cggatgaaat gtatttgtta atcatggtat aggcccttcc caggcggtaa
      661 tcgtcctcgc tcatctgggt tcccagccct gctttgatcc aggtctcgca ttcggggata
      721 tcgacgccga aactcccatc gagcgggtcg tagccgcggc catatgcttg catgcgggac
      781 aataccctgt tttccttgca ccacgcgaga aagggctcga caaatcgttc ccgcaaaagc
      841 tccgttttcg tccggtcgaa atcgaagcgc actcgcccgg tcaactgcct gaagacctcg
      901 ccatgctttg cgccgtactc cgggtcgata tcatttccca tccggcctgt tttataaagg
      961 atgaacggca aataaggttt cagttcatat ccccttcggc tcaggaactc tttttccata
     1021 tcatcgcacc agtttgctcc ctcgagctcg agactgtcgg tgaagaaaca gcggatatgg
     1081 ctcgtcagcg gcccgagcgc gtcagacatc cggtgcagat atttttcgac ggccgccttg
     1141 ttgtagtggt ttaagaccgg cccgttcgct ccgggcgccc cgttgatgac ctcgctaaag
     1201 ccatcgaact ctaccaatgc atacaacata tggctgccgg aaggtacggc tattgtgtac
     1261 gtcatcctcc ccggctctac cgggatctct ttgacctctt ccaggttgtt caatggggtt
     1321 ggaacgagcg aaagggagat cagtttggac acgcgtccgg caaatggcgc ggaaatcgcg
     1381 ggataggcgt ctttgatcag atccgccacc aacgccggat agtccgccgg tccgttcagt
     1441 ttgaccgctg aaatgaccct cacgcgactc ctctcttccc cggtaagcca gggcgcgccg
     1501 aatggccacc ccgatccaac gatcagatcg catgtcaaac cgagtgtgcc ggcttcctcc
     1561 agggtaaatc tcaggagctc cagccatgcc gggctcaacc aatcgacgga gcggataccc
     1621 atgtctgcag ttcgttcggg aaacgcgatc gggttgattt caacgccgcc gaaaccggcg
     1681 tctttcagga gccgaagctc gcgcgccagt tcggactttt ctatcttatc gccgttccac
     1741 caccaacggg caaacggccg gctggccatc gccgggttcc ggaacaactt gtaaagcctg
     1801 tcctctgaaa gcggccaata cctggcaacc gccgtcggca ctgccgctcc aagcgtgtac
     1861 ccgcctgcca acaacgaacc acgcttaatg aaagatcgtc ttctcaatga atatttgatt
     1921 tgcagcgatc ctgtattgaa aaacggcagc cctcagcggg gggctcttat gtctaggaga
     1981 atcctcgtaa tgcacgggcg atcgctgaat aaatcagcgg attctgttca ttgttgttga
     2041 gcagatctcc gggtttcagg ctgctcaaat aaggttcggg gaggatgttc ggctcgccgt
     2101 tgaaaacgtt tccgtcaggc atgtaggcgc aggtcatcgc ccgccgccac ccattggtca
     2161 tattggcgcc agctccatga atggtcagcc cgttgtgaaa ggaacagctg cctgccctca
     2221 tcggcgcggc aaccgccgga gttttcccaa atttcgggta gacatcgaaa atactgtcca
     2281 tattccggcc gatcgttatt ttgtcaaaat tcgtcccttt gtgcgaccca ggaataaaat
     2341 agaggcagcc gttttgtagc gtcgcgtcgt cgagcgccac ccagatcgaa atggccctcc
     2401 ggtcggagaa ggaccagaat ggggtatcga ggtgccatgc cgtgggattg gcccagggcc
     2461 gtttgaagag ggcctggtca tgccagatcc tgatcccttc cactcccgcc aagccggcgg
     2521 ccatccgccc gatccgctcg tccagcataa gctccttt
//
LOCUS       DATGKE010000634         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1154619, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000634 DATGKE010000000
VERSION     DATGKE010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154619"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VKU83_10710"
     CDS             <1..373
                     /locus_tag="VKU83_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94075.1"
                     /translation="GLPGDGVKLHPGIDYNELPDILAKYDVGLILYTGHILNWVDNAP
                     NKLFEYWSAGLDVWFSGKMTGSLPYVTTKTYPKVLAVDFENLEHFDWRAAVDRSGLEY
                     AASPYFCEDICRPLWNALLEA"
     gene            349..1545
                     /locus_tag="VKU83_10715"
     CDS             349..1545
                     /locus_tag="VKU83_10715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI94076.1"
                     /translation="MECIAGSLNKNADDNAMMEAGKKILLFADWYEPGYKAGGPIRSC
                     VNFARYMRGDYRIYVFTSDRDLRSPEPYEGVTVNEWQRDKHGINVYYCSPGRLTWQNI
                     RTQMRVVQPDFIYLNSMFSVKFTIYPLLITRICHFDHIVVLSPRGMLRKSAIQFKAMK
                     KRSFLRAFRLLGLQRSVRFHATDETEARDIGRYFGKGTRVSMVPNFPGPLIDDFPVPA
                     KVPGELALLFIGRIHPIKNLHLLLEILAKVEASVQLTIVGSLEDSTYWEKCRKLMAEF
                     PESIRVDYAGEMANHELPAIIAKHHIFALPTKGENFGHAIFEALALGKPVLISDQTPW
                     RGLHAAGAGWDLSLDRPEDFRLAIEEAAGYGRKEYEDLCASTWRYIGDYIAKLDLKDE
                     YKKLFR"
     gene            1546..>2354
                     /locus_tag="VKU83_10720"
     CDS             1546..>2354
                     /locus_tag="VKU83_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HLI94077.1"
                     /translation="MYILGLNAYHADSSAAIFKDGKMIAATEEERFRRVKHWAGFPSM
                     AIEFCLREAGIRLSDVDHIAIGRDPYAKLGKKLLYLAKNPGGGWKAVGDRLQNAKKVS
                     SLEDEFAAIDKSVDLRALKKKIHQVEHHRSHLASAFFASPFEEAALLSIDGSGDFTTT
                     MVGAGKANKIKVLDSVDFPHSVGIFYTAFTQLLGFPHYGDEYKVMGLAPYGRPEYVDR
                     LRDVLLFLPNGLFRINPRYFRSARSGIISYGDDHIPVVAPLYSGHLIEKFGP"
BASE COUNT          548 a          609 c          653 g          544 t
ORIGIN      
        1 tggtttaccc ggagacggcg tgaagctgca tccgggcatc gactataatg aactgccgga
       61 catcctggca aaatatgatg tcgggctgat cttatacacc ggccatatcc tgaattgggt
      121 ggacaacgcg cccaataaac tgtttgaata ttggagcgcg ggcctggacg tttggttttc
      181 cgggaagatg accggtagcc ttccctatgt aaccacgaag acctatccca aggtgctggc
      241 agtcgatttc gaaaacctgg agcatttcga ttggcgggct gcggtggaca ggtccgggct
      301 ggaatatgcg gcctcccctt atttctgcga agacatttgc cggcctctat ggaatgcatt
      361 gctggaagcc tgaacaaaaa cgcggatgat aacgccatga tggaagcggg taaaaagatc
      421 cttttatttg cagactggta tgaaccgggt tacaaggcgg gaggccccat ccggtcctgt
      481 gtcaattttg cccgctatat gcggggggat taccggatct acgtttttac gtccgaccgc
      541 gatctgcgat cgccggaacc gtatgaagga gtgaccgtga acgaatggca gcgggataaa
      601 catggtatca acgtttacta ttgctcgccg gggaggttga cctggcaaaa tatccggacg
      661 caaatgcgcg ttgttcaacc ggattttatt tacctgaata gtatgttctc ggtcaaattc
      721 acgatttatc ccttattgat aacccgaatc tgtcatttcg accatattgt cgttctatca
      781 ccaagaggca tgctccggaa gagcgcgatc cagttcaagg ccatgaagaa aagatctttt
      841 ttaagggctt tccggctgct gggcctgcaa cggtccgtcc ggtttcatgc gacggatgaa
      901 acggaggctc gggatattgg caggtatttc ggcaaaggaa cccgggtttc catggtccct
      961 aattttccgg gcccgctgat cgatgatttt cccgttcccg ccaaagttcc cggcgagctg
     1021 gccctcctct ttatagggcg gatccatccg atcaaaaacc tccatctgct cctggaaatt
     1081 ctggccaaag tagaggcaag cgtacaactg acgattgtcg gcagccttga agactcgacc
     1141 tactgggaaa aatgcaggaa gctgatggcg gagtttccgg aatccatccg ggtggattat
     1201 gccggcgaaa tggcaaacca cgaattaccg gccataattg ccaaacatca tatttttgcc
     1261 ttgcctacca agggggaaaa tttcggccac gcgatatttg aggcgctggc gctcggcaag
     1321 ccggtgttga tcagtgacca gacgccctgg cgcgggcttc acgccgccgg cgcgggttgg
     1381 gatctgtcgc tggaccgccc ggaggacttc aggctggcga tcgaagaagc agccggttat
     1441 ggccggaaag aatacgagga tctgtgcgcg tcaacctgga gatacattgg agattatata
     1501 gcaaaattgg atttgaagga cgaatacaaa aaactctttc gttaaatgta cattttaggg
     1561 ctcaatgctt accatgccga ctcatctgcg gccattttca aggatgggaa aatgattgcc
     1621 gcgacggaag aagagcggtt ccgcagggtc aagcactggg caggatttcc gtcgatggcc
     1681 atcgaatttt gcctgcggga agcaggcatc cgcctttccg atgtggatca tatcgccatt
     1741 ggccgcgacc cctatgccaa actcggaaaa aaactgctat acctggcgaa gaaccccggc
     1801 ggtggctgga aggcagtcgg cgatcgcctg caaaatgcga agaaagtatc ctcgctggaa
     1861 gacgaatttg cggcgatcga caagagcgtt gatctccggg cgctgaagaa gaagatccat
     1921 caggtcgagc atcaccggag tcatctggct tccgcctttt tcgcctcgcc tttcgaagag
     1981 gcggccctgc tctccatcga cggctcaggc gactttacca ccaccatggt aggcgccggg
     2041 aaagcgaaca agatcaaggt gctggactct gtggattttc ctcacagcgt cggcatcttt
     2101 tataccgcat tcacgcaact gctggggttc ccgcactacg gggacgaata taaggtaatg
     2161 ggactggccc cttatggccg cccggaatac gttgaccggc tccgggatgt gctactgttc
     2221 ctgcccaacg ggcttttcag gatcaatccc cgatatttcc gttccgccag aagcggcatc
     2281 atctcgtatg gggacgacca tattcccgtg gtcgctccgc tctattcagg ccatctcatc
     2341 gaaaagttcg gccc
//
LOCUS       DATGKE010000635         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1488524, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000635 DATGKE010000000
VERSION     DATGKE010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488524"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..884
                     /gene="rsmI"
                     /locus_tag="VKU83_10725"
     CDS             183..884
                     /gene="rsmI"
                     /locus_tag="VKU83_10725"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLI94078.1"
                     /translation="MLYIVPSPIGNLQDITLRALEVLKQVDVVLAEDTRNSSRLLNHY
                     QLIKPLSPYHAHNEHRVLHHLVDQLLQGKKMALLTDAGTPGVSDPAFLLVRECIKAGV
                     TVECLPGATAFVPALVNSGLPMNRFAFEGFLPVKKGRQSLLKQLAAEERTLIFYESPQ
                     RLVKTLEEMSRYFGAGRRCSVSRELTKVYEENRRGSLEEVCAHFREKGVKGEIVIVVE
                     GRPDERRQEIGEADV"
     gene            919..1374
                     /locus_tag="VKU83_10730"
     CDS             919..1374
                     /locus_tag="VKU83_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94079.1"
                     /translation="MKKTLFVLLTFVALVAGDRASAQFRSIPGAVTDSFKVRYPTATD
                     VNWSDKISAFQATFRQNGESFTTRYTKDGHWMYSTRKIAKDAIPAPVKDGLSKSKYAG
                     AEWEIRAVTVSYLPGNVTQYTIQVAKSDLQKKNLLFSSEGQLLKDNATL"
     gene            1371..>2135
                     /locus_tag="VKU83_10735"
     CDS             1371..>2135
                     /locus_tag="VKU83_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94080.1"
                     /translation="MIKKRILLAGMIGVLYLTVSAQSDRWQQRVKYTMNISMDVQSNR
                     FTGSQTLAYTNNSPDTLYKVFYHLYWNAFQPGSMMDNRSRELGKVVYSSGRRGGGQER
                     DWDSRVRDRILNLKPDEIGYEKVTSLTMNGAPQPFKIDETIMEVPLTKPILPRATVTF
                     QLQWESQVPLQIRRSGRDNPQTGVRYSMSQWYPKLCEYDYEGWHPTPYVAREFYGVWG
                     DYDVTISIDRHYLVAGTGYLENAAHVGFGYEAPGTKV"
BASE COUNT          519 a          491 c          663 g          462 t
ORIGIN      
        1 atgtccggat cggaaagagt atctcgctgc agatagaagc ggcttccgcg gatgaagcca
       61 ggcagatcgc agaggaagcc ggccggaaat tgctggccaa tccggtgatg gagtattttg
      121 aggctaccgt gtcataaacc acttgcggtg gtcgagcctt cgagaataaa aaaaacgaac
      181 ttttgctcta cattgtccct tctcccatag gtaatctgca ggatattacg ctcagggcat
      241 tggaggtgct gaaacaggtc gatgtagtcc tggcggagga tacccggaat tcgagccggc
      301 tgctcaatca ttaccagctc atcaagccgc tttcgcctta tcacgcgcat aatgaacatc
      361 gggtcctgca tcatttggta gaccaactgc ttcaagggaa gaagatggca ttgttaaccg
      421 atgccggcac tcccggtgtc agtgatccgg catttttatt ggtgagggaa tgtataaagg
      481 cgggtgtcac ggtggaatgt ctgccgggcg cgacggcatt tgtgcctgcc ctggtgaaca
      541 gcgggctgcc gatgaaccgg tttgcgttcg aggggttcct gccggtgaag aaaggccggc
      601 aatcgttgct gaagcaactg gctgcggaag agcggaccct tatcttttac gaatccccgc
      661 aacggttagt aaagacgctg gaggagatga gccggtattt tggcgcgggc cggcggtgca
      721 gcgtgagccg cgagctgacg aaggtctatg aggagaatcg aaggggttcg ctggaggaag
      781 tttgcgccca tttccgggaa aagggtgtga agggggagat cgtgatcgtt gtggaaggga
      841 ggcccgatga gcgccggcag gagatcgggg aggcggacgt gtaaaggagc tgtgaacaat
      901 aatttaaata tatcttttat gaagaagacc ctttttgtgc tacttacctt tgtagcgctt
      961 gttgcaggtg accgcgccag cgctcagttc cggtccatac ccggtgctgt aaccgattcc
     1021 ttcaaagtaa ggtatcctac agcgacggat gtcaactggt cggacaagat ctccgctttt
     1081 caggcgacat tcaggcagaa tggagagagt ttcaccaccc ggtacaccaa agacggacat
     1141 tggatgtatt ccaccaggaa gatagctaag gatgccatcc cagcacctgt gaaggatggt
     1201 ctatcgaaga gcaagtatgc cggcgctgag tgggagatcc gggcggtaac ggtatcttat
     1261 ttgccgggga atgtgaccca atataccatt caggtagcga agagcgatct gcagaaaaag
     1321 aatttattgt tctccagcga gggacagctg ttgaaagaca acgcgacatt atgataaaaa
     1381 aaaggatcct acttgcgggg atgatcggcg ttctctatct cactgtatcg gcgcaatccg
     1441 atcggtggca acagcgggtg aagtacacga tgaatatctc catggacgtg cagagcaacc
     1501 gctttaccgg cagccagacg ttggcttaca ccaacaattc ccccgacacg ctttataaag
     1561 tattctatca cctgtattgg aatgcttttc agccgggaag catgatggat aaccggagca
     1621 gggagctggg gaaggtcgtg tattccagtg gacggcgggg tggcggtcag gagcgggatt
     1681 gggatagccg ggtgagggat aggatactga acctgaaacc ggacgagatc ggatatgaga
     1741 aagtgacttc gctcaccatg aacggggcgc cgcagccttt caagatagac gagaccataa
     1801 tggaagtgcc gctcaccaag ccgatcctgc cgagggcgac ggtcacgttc cagctgcagt
     1861 gggaatcgca ggtacccttg cagatccggc ggagcggcag ggacaaccca cagacaggag
     1921 tgcgctattc catgagtcag tggtacccta agctatgcga atacgattac gagggttggc
     1981 atcctacgcc atatgtagcg cgggagtttt acggtgtttg gggcgattat gacgtgacga
     2041 tctccatcga ccggcattac ctggttgcgg gcacgggcta tctggaaaat gcggcccatg
     2101 tcggtttcgg gtatgaggcg ccggggacga aggtg
//
LOCUS       DATGKE010000636         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1183887, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000636 DATGKE010000000
VERSION     DATGKE010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183887"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VKU83_10740"
     CDS             complement(<1..357)
                     /locus_tag="VKU83_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94081.1"
                     /translation="MDKFSSQFIPMPLRHFVLCCFLFVLVGPLRAQAKTDSFLVSLLR
                     SHASPALIHVLDNPDSFRYQLIYTRIDRDKHNRPHFHNYYYRVNPRDYFNPASSVKMP
                     LAFLALEKMDSLARYGV"
     gene            443..1450
                     /locus_tag="VKU83_10745"
     CDS             443..1450
                     /locus_tag="VKU83_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLI94082.1"
                     /translation="MKIVSYLKAEHDQLALLVDDLLYDVELIHPDIPNTMNMFLSYWD
                     ELFPVLQAGEIAIGDGHISATKAIPVDQVRLLAPVPFPASCRDGYAFRQHVAAARRNR
                     KVPMIPEFDQYPVFYFTNHHSVQGPGEIRCMPDHFNKLDFELEVAIVIARQGRNIPAA
                     EADGYIAGLMIMNDMSARTLQMEEMLLNLGPAKGKDFATATGPWLVTLDELRDLEIPC
                     KEGHTGLNWNLRMRCRVNEVTVSDGNLGDMDWTFAELIERASYGADLYPGDLIGSGTV
                     GTGCFLELNGTGKLSDPGYREQWLQEGDRIEMEVDGLGILENSIVREDSELSLLEGKK
                     K"
     gene            1447..2331
                     /locus_tag="VKU83_10750"
     CDS             1447..2331
                     /locus_tag="VKU83_10750"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLI94083.1"
                     /translation="MTVDLNKLGPAERQGFLNASVAPRPICFASTIDLSGQVNLSPFS
                     FFNVFSTTPPVLIFSPSRRVRDNSTKHTLHNVLEVPEVVINIVDFPMVQQVSLSSCDY
                     PKEVSEFVKAGFTEEAATLVRPPMVKESKIRFECRVMEVKVLGDKGGSGNLVICEPLR
                     MHVDDRVLDPATGRIDQSRIRLVARLGEDWYCAVGPDNLFRVEKPNTQLGIGIDALPA
                     AIRDSRILTGNDLGRLANVQALPVVDPAYHDERLQQIFQYYAVNPEEMEVELHSYARS
                     LLEAGKVREAWQVLLALN"
     gene            2419..>3273
                     /locus_tag="VKU83_10755"
     CDS             2419..>3273
                     /locus_tag="VKU83_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HLI94084.1"
                     /translation="MTPNLSEQEQIRRDKLAELQQLGIDPFPAASYPVNATAARIKEH
                     YPAVEMPDVCVAGRLMSRRDMGKAYFADLQDSTGRIQLYIRRDDICPGEDKTLYNKVW
                     KHLLDLGDIIGVKGFVFTTKMGEISIHVREFVLLTKALKPLPVVREKEGATFDAVTDP
                     EFRYRQRYADLIVNPQVKDVFVKRSRMINAMRQFLNDRGALEVDTPVLQSIPGGAAAR
                     PFVTHHNALDIPFYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPEFTVLE
                     FYTAYKDYL"
BASE COUNT          731 a          949 c          919 g          674 t
ORIGIN      
        1 gactccatac cgcgccaaag aatccatttt ctccagcgca agaaaagcaa gcggcatctt
       61 cactgaagac gccgggttga aataatcccg cgggttaacc cggtaatagt agttatgaaa
      121 atgcggccgg ttatgcttgt cgcggtcgat gcgggtgtag atcagctgat accggaagga
      181 gtcgggatta tccagcacat ggatgagggc cggagaggca tgggaccgca acaggctgac
      241 caggaagctg tcggttttag cctgcgcccg cagcgggcca acgaggacga acaggaaaca
      301 gcaaaggacg aaatgacgaa gcggcatcgg gatgaattgc gacgagaatt tatccaaaaa
      361 tattgttttt tgtcagatta ccttcctgtc catccttccg gcctcgccgg ctgaacaaga
      421 aaattgcttt acttttactt ccatgaagat cgtctcttat ctgaaggccg agcatgacca
      481 gttggcgctg ctcgtcgatg acctgctcta tgacgtcgag ttgatccatc ccgacatccc
      541 gaacacgatg aacatgttcc tcagctattg ggatgaactt tttccggttc tccaggccgg
      601 ggagatcgcg atcggcgatg gacatatcag cgcgaccaaa gccatcccgg tggaccaggt
      661 gcggctgctc gccccggtcc cgtttcccgc ctcatgccgc gacggctatg cattccggca
      721 gcacgtagcc gccgcccgcc gcaaccggaa agtgccgatg atcccggaat tcgaccaata
      781 cccggttttc tacttcacga accaccacag tgtgcagggc cccggcgaga tccgctgcat
      841 gccggatcat ttcaacaagc tggatttcga gctggaagtg gcgatcgtga tcgcccggca
      901 aggccgcaac attcccgccg ccgaggccga cggctatatc gccgggctga tgatcatgaa
      961 cgatatgagc gcccgcacgc tccagatgga agaaatgctt ctcaatctgg gtccggccaa
     1021 aggaaaggat ttcgctacgg ccaccggtcc ctggctggtg acgctggacg aattgcgtga
     1081 cctggagatt ccgtgcaaag aggggcacac gggcctgaac tggaacctgc ggatgagatg
     1141 ccgcgttaac gaggtcacgg tcagcgacgg taacctgggc gacatggact ggacattcgc
     1201 cgagctgatc gaacgggcgt cctacggcgc cgacctttat ccgggcgatc tgatcggcag
     1261 cggtaccgtt ggcaccggct gcttcctcga attgaacggc acgggtaaac tcagcgatcc
     1321 cggttatcgg gaacagtggt tacaggaagg tgaccgcatt gaaatggaag tggatgggct
     1381 cggcatcctt gaaaactcca tcgtgcggga agacagtgag ctttcattgc tggagggaaa
     1441 gaaaaaatga cggtcgacct gaacaagctt ggccctgccg aacgccaggg ctttttgaac
     1501 gcttcggtgg cgccgcggcc catctgcttc gccagcacca tcgatttgag cgggcaggtc
     1561 aacctcagcc cgttcagctt ctttaacgtt ttctcgacca cgccgccggt cctgatcttt
     1621 tccccttcgc gccgggtcag ggacaattca accaaacata cgttgcacaa cgtactggaa
     1681 gttcccgagg tcgtcatcaa tatcgtcgat ttcccgatgg tccagcaggt aagcctttcg
     1741 agctgcgatt atcccaagga agtctcggag ttcgtcaagg cggggtttac cgaagaggcc
     1801 gccacgctcg tcaggccgcc gatggtaaag gaaagcaaga tccgcttcga atgccgggtg
     1861 atggaagtaa aggtcctcgg tgacaagggt ggctccggaa acctggtcat ctgcgaaccg
     1921 ctgcgcatgc acgtcgatga ccgtgtcctg gatcccgcta ccggccgcat cgatcagagc
     1981 cgcatccgcc tcgtcgcccg cctgggtgaa gactggtatt gcgctgtcgg gccggacaat
     2041 ctttttcgcg tggagaaacc caatacgcag ctcgggatcg ggatcgacgc cttgccggct
     2101 gccatccggg acagccgcat cctgacgggc aacgaccttg gccgcctcgc gaatgtgcag
     2161 gctttgccgg tggtggaccc cgcctatcac gatgagcggc tccaacagat cttccagtat
     2221 tatgccgtca acccggaaga gatggaggtc gaactgcatt cctacgcccg ctcactactc
     2281 gaagccggca aggtacgcga agcctggcaa gtactgcttg cgttgaacta gcgcgtcgtg
     2341 ttcaccaatc cttggcgcct ccgggcatat actgtacatt tgccggaatt tccaatttct
     2401 acttggttta tgccgacaat gacacccaac ttatcggaac aggaacagat caggcgcgat
     2461 aagctcgcag aactgcagca gctcggcatc gacccttttc cggctgcttc ttacccggta
     2521 aacgccaccg ccgcccgaat caaggagcat tacccggcag tggaaatgcc ggacgtctgc
     2581 gttgccggcc gcctcatgag ccgccgcgac atgggtaaag cctatttcgc cgatcttcag
     2641 gacagcaccg gcaggatcca gctctatatc aggcgggacg atatctgtcc cggcgaagac
     2701 aagacccttt ataacaaggt ttggaaacac ctcctggacc tcggtgatat catcggcgtc
     2761 aaaggattcg tattcaccac aaaaatgggt gagatctcca tccacgttcg cgaattcgtg
     2821 ttgctgacga aggccctgaa accgcttccc gtcgtccgcg aaaaggaagg cgccactttc
     2881 gacgccgtca cggatccgga attccgttat cgccagcggt atgcagacct gatcgtcaac
     2941 ccccaggtga aggacgtttt cgtgaaacga agccggatga tcaacgccat gcgtcaattc
     3001 ctgaacgacc gcggggcgct ggaagtcgat acgccggtcc tccagagtat tccgggaggc
     3061 gccgccgcca ggccatttgt cacccatcat aatgcgcttg acatcccgtt ctaccttcgg
     3121 atcgcgaatg aactgtacct gaagcgcctg atcgtcggcg gttttgactg ggtttatgaa
     3181 ttcagccgca atttccgcaa cgagggaatg gaccgtacgc ataacccgga gttcacggtg
     3241 ctcgagttct ataccgctta taaagactat ctc
//
LOCUS       DATGKE010000637         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_80451, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000637 DATGKE010000000
VERSION     DATGKE010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80451"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3367)
                     /locus_tag="VKU83_10760"
     CDS             complement(<1..3367)
                     /locus_tag="VKU83_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLI94085.1"
                     /translation="MIKDVDERQMGFRVAGVELYIQTSYDLSVVVNIREEGRIKFYYN
                     ANAIESRRIRRMAAHFDRVLKQVSAAPEMAITRIEILLPEERQQLLNWSVGPEITYPR
                     SATVTGLFAHSARLFPDRPALRYKGSEMTYKELHERSNQLAHFLRDRGVRQGTVVGLM
                     MERSMELIIGLLGILKAGGAYMPMEPGTPEKRMRHMLEESRALLLLTGMDDDELYSYP
                     TGEVKVNVSPESNAYILYTSGSTGKPKGVMIPHSALTNYICWASSEYAAGERVLFALF
                     SSIAFDLTVTSIFCPLVTGNTLEIYEEGNSAWLIETIIEEDKASVIKLTPSHLKIILG
                     SRMVRHAPKKNLRRFIVGGEELTTALAKDIYDLFGGRVELINEYGPTEATVGCMIHRF
                     RPDEAMAAVPIGVPAANVRIYVLDRHQTLAPAGTIGEMYISGAGIARGYVNNEELTKE
                     RFLDDPFAPGMRMYRTGDLARWLDDSKLEFLGRTDEQVKIKGFRIELGEITNCLRLHR
                     QVKDAIVLTWEAEGSQNLVAYYVSEGEIQTDGLRLFLSARLPDYMLPAFFVRVERMPL
                     TTNGKLDKKALPAPQRTPIIAEEVSTAEERLLVSIWEKVLGREGIGVTENFLSLGGDS
                     IKSLQLVSRLYTAGYEITVKEIFLHPTIRQLAVKLRKIGMASDQSVVREESGLTPVQQ
                     LFFEGVIGNKDHYNQSVLLHFGSGISGDEVRRIGEKLQEHHDALRMVFVREGDKVVMR
                     NRGLETEVRVQEADLTGSKDADMELLRRCNSVQGSIDLAAGPLMKLGLFHMEGGSRLL
                     IVIHHLVVDGVSWRILLEDIDTLYRQLKAGAPLSLPQKTASFVSWPSQLSEYRKSAAF
                     SRGRVYWSGELGRKEAMVPRDYPEGKNIAEESRVERMQLDSLSTELLLTKANDSFGTR
                     VNDLLLCAFLLSIRRQYGMNGIRVDLEGHGREEVGQGMDVSRTVGWYTSLYPVVLESA
                     SEQLPRLIKEVKETLRRVPNNGIDYLLLNYGDKQAPVMETGRSRIVFNYLGQFDTDIR
                     NRAYSIAAESSGEEIDPGSENEYDWQLSGLVKGGVLEISLRFSNSQYKPEAIRSLMDH
                     YRDALASIISFCCRYGKTELTPSDLTYKRLPVEE"
BASE COUNT          736 a         1136 c          733 g          821 t
ORIGIN      
        1 gctcttcaac cggcaggcgc ttataagtaa ggtccgaagg ggtcagttct gtttttccgt
       61 accggcaaca gaaggaaatg atcgatgcaa gcgcatccct gtaatggtcc atcaacgacc
      121 ggatcgcttc gggtttgtac tggctgttgc tgaagcgaag gctgatctcc agcacgccgc
      181 ccttcaccag ccccgatagc tgccagtcat attcattttc cgaacccgga tctatctcct
      241 ctccggacga ctccgcggca atcgaatagg ccctgttccg gatgtctgta tcgaactgac
      301 ccaggtaatt gaacacaata cgcgaccggc ccgtctccat caccggcgcc tgcttgtccc
      361 catagttcag cagcagatag tctatcccat tattcggaac ccgccgcaag gtctctttta
      421 cttccttgat cagccgcggc agctgctccg atgcactctc cagcaccacc ggataaagac
      481 tcgtgtacca tcctaccgtc cggcttacat ccatcccctg acccacctcc tcccggccat
      541 gtccttccag gtccacccgt atcccgttca tcccatactg tctgcggata ctcaacagga
      601 acgcacacag cagcaagtca ttgacccgcg ttccaaagga atcatttgcc ttggtcagca
      661 acagctccgt cgacaagctg tccagctgca tccgttccac ccggctctct tccgcaatat
      721 tctttccctc cggataatcc cgcggtacca tcgcctcttt tcttcctagc tctcccgacc
      781 agtagacgcg gccccgcgaa aaagcagcgc tcttacggta ttccgacagc tgcgaaggcc
      841 aggacacgaa cgatgcggtc ttctgcggca gggacagcgg cgcccccgct ttcagctgac
      901 ggtaaagcgt gtcgatgtct tccagcagga tccgccagct cactccatct accaccaggt
      961 gatggatcac gatcagcagc cggctgcccc cctccatatg gaacaaaccc agcttcatca
     1021 acggacccgc cgccaggtcg atcgatccct gcacgctgtt gcatctcctt aacagctcca
     1081 tatccgcatc cttgcttccg gtaagatcgg cctcctgcac ccttacctcc gtctccaacc
     1141 cccggttccg catcaccacc ttgtcccctt cccggacaaa taccatccgc agggcatcat
     1201 gatgttcctg cagcttctcc cctatccttc gcacctcgtc accgctgatc cccgatccaa
     1261 aatgcagcaa caccgactga ttgtaatgat ctttgttccc aattacccct tcgaagaaca
     1321 attgctgcac aggtgtcagc cccgactctt cccgcaccac cgactgatcc gatgccatcc
     1381 cgatcttcct gagttttact gccaactgcc ggatggtcgg atgaagaaag atctctttta
     1441 ccgtaatttc ataccccgcc gtatacagac gcgatacgag ctgcagcgat ttgatggaat
     1501 ctccccccag cgacaggaaa ttttccgtta ctccgatccc ttcccgccca agcacctttt
     1561 cccaaatgct taccagcagt ctctcctccg ctgttgacac ctcttccgct atgatcgggg
     1621 tcctttgagg cgccggcaag gcctttttat ccagctttcc gtttgtggtc aaaggcatcc
     1681 gctccactcg cacaaagaaa gccggaagca tgtaatccgg cagcctggcc gataagaaaa
     1741 gccgtaaacc atccgtctgg atctcgcctt ccgatacata ataagccacc aggttttgac
     1801 tgccttcggc ttcccacgtc agcactatcg catccttcac ctgtctgtga agccgcaggc
     1861 aattagtgat ttcccctaat tcgatccgga atccttttat tttcacctgc tcatccgtcc
     1921 taccgaggaa ttccagcttg ctgtcatcca gccagcgcgc caggtcgccc gtcctgtaca
     1981 tccgcattcc gggagcaaac ggatcatcca ggaatctttc ttttgtcagc tcctcattgt
     2041 tgacgtagcc gcgtgcgatg cccgcccccg agatatacat ctctccgata gtcccggccg
     2101 gagccagggt ttgatgcctg tccaatacat agatccttac attggctgcc ggcacgccta
     2161 ttggtacggc agccatcgct tcatccggcc ggaaccgatg gatcatgcac ccaaccgtcg
     2221 cctcggtcgg gccatattca ttgataagtt ctacccttcc tccaaacaga tcataaatat
     2281 cctttgccag cgccgtcgtc agctcctctc ctcctacgat aaatcgcctg agattcttct
     2341 ttggcgcatg ccgtaccatc cttgagccca ggatgatctt taaatgcgaa ggtgtaagct
     2401 taataacgga cgccttgtcc tcctcgatga tcgtttcgat gagccacgca ctgttgcctt
     2461 cttcatagat ctccagcgta ttgcctgtaa ccagcggaca gaagatcgac gtcaccgtaa
     2521 gatcgaatgc gatcgagctg aacaacgcaa aaagcactct ttcaccagcc gcatactcgc
     2581 tgctggccca gcaaatgtaa ttggtaagcg cactgtgcgg gatcatcact cccttaggct
     2641 tccccgtcga accggaagta taaaggatat acgcattact ttcgggcgat acgtttacct
     2701 tcacctcccc ggttggatag gaatatagtt catcgtcatc cattcccgtt aaaagcagca
     2761 gcgccctact ttcttcgagc atgtgacgca tacgcttttc gggagtgccc ggctccatcg
     2821 gcatataagc gccccctgcc ttcaatatac ccaacagccc tatgatcagt tccatcgatc
     2881 gctccatcat caaccccacc acggtgccct gccgtacccc tctgtcccgc aggaaatgcg
     2941 ccagctggtt ggaccgctca tgcagctctt tatacgtcat ctccgatccc ttataccgca
     3001 atgccggccg gtccggaaat aacctggcgc tatgagcaaa aaggccggtc accgtagcgc
     3061 tacggggata ggtgatctcc ggtccgacag accaattcag caactgctgt ctttcctccg
     3121 gcaatagtat ttcgatccgc gttatcgcca tttccggggc agcggacacc tgtttcagca
     3181 cccggtcgaa atgcgccgcc attcgccgga tgcgccggct ttcgatcgca ttggcattat
     3241 aatagaattt tatgcgtccc tcttccctta tattgactac aacagaaaga tcatagcttg
     3301 tctgaatgta tagctccacc ccggcaaccc ggaaccccat ctgacgctca tcgacgtctt
     3361 ttatcatatc ggttatgggg taattttcaa acaccaggat atggtcgaac agatcccggc
     3421 cgggct
//
LOCUS       DATGKE010000638         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_142584, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000638 DATGKE010000000
VERSION     DATGKE010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142584"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      76..141
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            177..545
                     /gene="crcB"
                     /locus_tag="VKU83_10765"
     CDS             177..545
                     /gene="crcB"
                     /locus_tag="VKU83_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HLI94086.1"
                     /translation="MKTLLLIALGGAAGSVGRYLAQLGVSKAFGPAFPVGTFIVNLTG
                     CFLIGLIYSLAGKYEWLTLDWRLFLVTGVCGGYTTFSSFSYDSVNLLRSGSYLYFGLY
                     VFGSVALGLLATFAGIRIAR"
     gene            561..1001
                     /locus_tag="VKU83_10770"
     CDS             561..1001
                     /locus_tag="VKU83_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI94087.1"
                     /translation="MPVKNILLLVSDSPESRKAAKFGFELARTLNAAAALVFVVDKSH
                     EMVNPDLGVSLEQKRAAMLKAARATIAQLTELYAAGSEVLQFIPEGRPGIEIIKVAAE
                     WPADLIVMGTHLHGRIELLLQGSLTEYIIRHATVPVIVAPPAMR"
     gene            complement(1010..1366)
                     /locus_tag="VKU83_10775"
     CDS             complement(1010..1366)
                     /locus_tag="VKU83_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI94088.1"
                     /translation="MKKVLVVDDDPDILQLVKIALTMNGLDVTTLAQWEKLDGAIREC
                     MPGLILLDISLGSADGREICRDLKMAEGTKDIPVILFSANVEFQRGIEEWKAESFIAK
                     PFELKKLVETVKAFAK"
     gene            complement(1414..2808)
                     /locus_tag="VKU83_10780"
     CDS             complement(1414..2808)
                     /locus_tag="VKU83_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI94089.1"
                     /translation="MLNSERVKGLNNISIRNKLVLMQVFTSILVLGIFATVYIVTEIN
                     NFKSRKANDIRSVAAVIAQNSASSLQFQDDETARQMLAELRSVTPDISFSAILDQHRQ
                     VFAAVGRKDADTAMFNEHRNSRGGTYFSDNTLVVVGPITYQADTIGTVYLQVRLDELG
                     RIKWYLLRLAIVLSLIAVLAAFVISFGLQAYISSRLLALVRAMRRVGDTGDYTTGLKD
                     EGDDEISVLINVFNSLLFQIRENERRKDEFIGVASHELKTPLTNVKGYLEILQTMEDR
                     PSQRQLVDRAFLNARKLEKLIADLLDVTKIQTGQLELNLTVFPLDELIAETIASTQML
                     AKDRPFVFHAKSGSEIRADRQRIEQVLLNLLSNAIKYSADNTPVAVTAVATAGQVVVE
                     VADCGSGVPEGEENAIFGRFYRAKGVSKHISGFGLGLYICRDIIHRHHGRIWVRNRPD
                     GASFFFQLPINGPP"
     gene            complement(2795..3328)
                     /locus_tag="VKU83_10785"
     CDS             complement(2795..3328)
                     /locus_tag="VKU83_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HLI94090.1"
                     /translation="MSRGFTYSAILTLLLLSGFRQAGAAQDEEVETNLKAAFLYNFTK
                     YIDWQLESGNGEFVIGVIGSSAVEPALEEIARTGTVDGRKITIRHIDRPDQIGGCQLL
                     FIAHRRHLGLQEILSRVTRGEIAVAEENGAARNGAGFNFVRSGDKLRFEANIGALAAS
                     GLKVSSQLLKLAILVKQ"
BASE COUNT          676 a          976 c          949 g          773 t
ORIGIN      
        1 agctcgcgac aacatttttg accgtcgacc ggcacgcccg atcatccctc acgagtatca
       61 ctttttttcg tacttttacc aaaaggtgat ggtgtccacc tattgaaccg ccgtgatccg
      121 gctgatggcg cctacaaaga attaataccg gaatgaatca tattaatccc ttgtagatga
      181 agacacttct ccttatcgcc cttggtggcg cggccggcag cgtgggccgt tatctggccc
      241 agctcggcgt ttccaaagca tttggcccgg catttcccgt cggaactttc attgtcaatc
      301 tgacgggatg ttttctcatt ggcctcattt actccctcgc cgggaaatac gaatggctga
      361 cccttgactg gaggctgttc ctggtaaccg gcgtctgcgg aggctacact accttctcga
      421 gcttctccta tgacagcgtg aacctgctga ggagcggcag ttacctgtat tttggactct
      481 atgtgtttgg cagcgtggcg cttggcctgc tggcaacctt tgccggcatt cgtatcgccc
      541 gatagtggta attttgtaaa atgccggtga aaaatatcct gctgcttgta agcgatagcc
      601 cggagtcccg caaagcggcc aaattcggct ttgagctcgc gcgcacgtta aacgccgctg
      661 ccgcgctggt cttcgttgtc gacaaatcgc atgaaatggt gaacccggat ctaggtgtca
      721 gcctggaaca gaaacgcgct gcgatgctaa aggccgcccg cgcgaccatc gcccaactga
      781 cagaactcta cgccgccggc agcgaggtcc ttcagttcat cccggaaggc cggccgggaa
      841 tcgagatcat taaggtcgcg gccgaatggc ccgccgatct cattgtcatg ggcacgcacc
      901 tgcatggcag gatcgagctc ttgcttcagg gctcgctgac ggaatacatt attcgccatg
      961 ccaccgtccc cgtcatcgtc gccccgccgg ccatgcgctg agggtgtttt catttcgcaa
     1021 atgcctttac cgtttcgacc agcttcttca actcgaatgg tttggcaatg aagctttccg
     1081 ccttccactc ctcgatgccc cgctgaaatt cgacattggc ggagaacagg atgacgggga
     1141 tatccttcgt cccttccgcc atcttcagat ccctgcagat ctcgcggcca tcggcgctgc
     1201 ccagggagat atcgagcagg atgaggccgg gcatgcattc gcggatggcc ccatcgagct
     1261 tttcccactg cgctaaggtg gtgacatcga gcccgttcat ggtcagggct attttgacaa
     1321 gttgaaggat atccgggtcg tcatcgacta cgaggacttt tttcatgagt gaacgattaa
     1381 tggatgaatg gtttccggcc aggccacggc cgttcagggc ggcccattga ttggaagttg
     1441 gaagaaaaaa gatgcgccgt ccggccggtt gcgcacccag atgcggccat gatgacgatg
     1501 gataatgtcc cggcaaatat acaacccgag gccaaaaccg gaaatatgtt tggagacccc
     1561 tttcgcccgg tagaaccggc cgaagatggc attttcttct ccttccggca ccccgctgcc
     1621 gcagtcggcg acctctacga cgacctggcc ggccgtcgcc accgccgtca cggcgacggg
     1681 agtgttgtcc gccgaatact tgattgcatt ggaaagcagg ttcagcagga cctgctcgat
     1741 ccgttggcgg tccgcgcgga tctcgctgcc ggactttgca tggaagacga acggccggtc
     1801 cttggccagc atctgcgtgg aagcgatcgt ttcggcaatc agctcatcga gagggaatac
     1861 cgtcaggttg agctcgagct ggcccgtttg gatcttggtc acatcgagca ggtcggcgat
     1921 gagcttttca agttttctcg cattcagaaa cgcccggtcc acgagttgcc gctgcgaagg
     1981 ccggtcttcc atcgtctgga ggatctcgag gtagcccttg acgttcgtca ggggtgtctt
     2041 cagttcatgg ctggccaccc cgatgaattc atcctttcgc cgttcgtttt cccggatctg
     2101 gaacagcagg ctgttgaaaa cattgatcaa cacactgatc tcatcgtctc cttcgtcttt
     2161 cagcccggtc gtataatcac ccgtatcgcc gacgcgacgc atggcgcgga cgagggccag
     2221 cagccgggat gaaatataag cctggagtcc gaacgagatc acgaaagcgg ccaggaccgc
     2281 aatcagcgac aggacgatgg cgagccggag caggtaccat tttatgcgcc cgagctcgtc
     2341 gagccggact tgcaaataaa ccgtaccgat ggtatcggct tggtaggtga tcggtccgac
     2401 aaccaccagg gtattgtctg agaagtatgt gccgccgcga ctgttccggt gttcattgaa
     2461 cattgcggtg tcggcgtcct tccggcccac cgccgcaaag acctggcgat gctggtccag
     2521 gatggccgaa aagctgatgt cgggcgttac cgagcgcagt tccgccagca tttgccgggc
     2581 ggtctcatcg tcctggaact gcagggaaga ggcactgttc tgagcgatca cagccgctac
     2641 gcttcggata tcgttcgctt tccgggattt gaagttgttg atctcggtga cgatatagac
     2701 ggtagcgaag ataccgagga cgagtatgct ggtgaagacc tgcatcagca ccagcttgtt
     2761 tcggatcgat atgttgttga gtcccttcac ccgttcactg tttaacaaga atggccagtt
     2821 tcaataattg cgagctgacc ttcaacccgc tggcggcaag cgcgccgatg ttcgcttcga
     2881 acctcagttt gtccccggaa cggacgaagt tgaagcccgc gccgttccgg gccgcgccat
     2941 tctcctcggc tactgcgatc tcgccccggg tcacccggga taaaatctcc tgcaatccga
     3001 gatgtcgccg gtgcgcgatg aacaggagct ggcaaccgcc gatctgatcg ggcctgtcga
     3061 tatgccggat agtgatcttc cggccgtcca ccgtgccggt tcgcgcgatc tcttccaatg
     3121 ccggttcgac cgcggaggac ccgatgaccc caatgacaaa ttcgccgttg ccggactcca
     3181 gctgccagtc gatatatttc gtgaaattgt agaggaatgc cgctttcagg ttcgtctcca
     3241 cctcttcgtc ctgcgcggcg ccggcttgcc ggaagcctga caggagcagg agcgtaagga
     3301 tggcggaata tgtaaacccc cggctcatag gctaatgcca agcttaatga tgccgttcgc
     3361 gcccggttgt tcca
//
LOCUS       DATGKE010000639         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_883754, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000639 DATGKE010000000
VERSION     DATGKE010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883754"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..714)
                     /locus_tag="VKU83_10790"
     CDS             complement(58..714)
                     /locus_tag="VKU83_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLI94091.1"
                     /translation="MNRKKIGFFIGFFVLLFFGFYFALSRLIPGYGTPTLPVLSQVPH
                     FAFDNQEGKRITDRDLLGKVYVAEYFFTTCKGICPILNTNMRKVAADYAGQPDFRILS
                     YTVDPETDSVARLKHYADSLGADPTRWWFLTGTKDSLYRLARNGYLLDDPKNNAININ
                     EQFLHTQFFALVDKGGRVRKIYDGLKQDELNELEKDIPVLLKEDKDAGRFANSLFKTP
                     "
     gene            861..1454
                     /locus_tag="VKU83_10795"
     CDS             861..1454
                     /locus_tag="VKU83_10795"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HLI94092.1"
                     /translation="MIRTGNPVISIYTEMTPNPETMKFVANKLLYPGKSIDFPDTEAA
                     RPSPLATELFGFPFIKSVFIASNFVTLTKTADTDWDDVIPAIRQFLKEYLEENKPVIN
                     EEEIVLVKPEGNHIDADDDDVVKRIKELLENYVKPAVEMDGGAIQFKSYHEGTVNLML
                     QGSCSGCPSSMITLKAGIEGMMKRMIPEVKEVVAETE"
     gene            complement(1480..2190)
                     /gene="mtgA"
                     /locus_tag="VKU83_10800"
     CDS             complement(1480..2190)
                     /gene="mtgA"
                     /locus_tag="VKU83_10800"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HLI94093.1"
                     /translation="MTTPNKRKRGLLSRIWRVSRRVLIILFVAQLLYIVLLKWVYPPI
                     TITEIGSLLRGDGLKRTYVGRKEISPNARLAVISTEDQLFPDHDGFDFKNIEKALKHN
                     QKSKSLHGASTISQQVAKNVFLWQGRSWFRKGLEVYFTSMIELIWGKKRILEMYLNVA
                     EMGKGIYGIEAASRFYFHIPARNLSRLQAARIAACLPNPKVYTVKPLSNYVATRSEWA
                     LTQMDRLQNDPDIRTLLQ"
BASE COUNT          546 a          594 c          609 g          541 t
ORIGIN      
        1 tttgagcagc gcatcgattt tcttttccat gataacctgg attgcaaagc cgccgtttta
       61 cggcgtcttg aataatgaat tggcgaagcg ccccgcatcc ttatcttcct taagcaatac
      121 cggtatatcc ttctccaatt cgttcagctc gtcctgcttc aacccgtcat agatctttcg
      181 cacccgaccg cccttatcca ccaatgcaaa gaactgggta tggaggaact gttcgtttat
      241 gttgatggca ttgtttttcg gatcgtcaag gagatagccg ttccgggcga gacggtaaag
      301 gctgtctttc gtgccggtga ggaaccacca gcgcgttgga tcggcgccca gcgaatcggc
      361 ataatgcttc agccgcgcga cactgtccgt ttcagggtcc accgtgtagg aaaggatacg
      421 gaaatccggc tggcccgcat aatccgcggc aactttcctc atgttcgtat tcaggatcgg
      481 acaaatgccc ttgcacgtcg tgaagaaata ttcagccaca tagacctttc ccaacaggtc
      541 cctgtcggtg atccgctttc cttcctggtt gtcgaaggcg aaatggggca cctggctgag
      601 gacgggcaat gtcggcgtgc cgtatccggg gatcaggcgg ctgagggcaa aatagaagcc
      661 gaaaaagaga aggacaaaaa acccgataaa gaagcctatt tttttcctgt tcataacagg
      721 acaaaggtag caatgcgcag gcgaatctgc ttttttgcaa ctcagttgca atttcgtaca
      781 tttgcggttc cgcagtcttc gatccgtatc tttgcagcgt cgcggacttg ttatttttgc
      841 acccaaataa acggcgatct atgattagaa caggaaatcc ggtcatcagc atctacacgg
      901 agatgacgcc caacccggaa acgatgaaat tcgtggccaa caagttattg tatcccggaa
      961 aaagcatcga tttccccgac acggaagctg ccaggccatc cccgctcgcc accgagctgt
     1021 ttggctttcc ctttatcaag agtgtgttca tcgccagcaa ttttgtaacg ctcaccaaga
     1081 ccgcggacac ggattgggac gacgtgattc cggcgatccg gcagttcctg aaggaatatc
     1141 tcgaggaaaa caagccggtg atcaacgaag aggagatcgt ccttgtcaaa ccggagggca
     1201 accatatcga tgcggatgac gacgacgtcg tcaagcggat aaaggaactg ctcgagaatt
     1261 atgtcaagcc ggccgtcgaa atggacggcg gcgctatcca gttcaagagc tatcatgaag
     1321 ggactgtcaa cctgatgttg cagggctcct gttccggctg cccctcctcg atgatcaccc
     1381 ttaaggcggg catcgaaggg atgatgaagc ggatgatccc ggaggtaaag gaagtcgtgg
     1441 ccgagaccga ataaagcctt tgtcgacgcg acgacgcgct tactgcagga gcgttcggat
     1501 atcgggatca ttttgcagcc ggtccatctg cgtgagggcc cactccgacc gggtggcaac
     1561 ataattggat aaaggtttga ccgtataaac cttcgggttg ggaaggcagg cggcgatccg
     1621 ggctgcctga aggcgcgaga ggttcctggc cgggatgtga aaatagaagc gggacgccgc
     1681 ttcaatgcca tagatccctt ttcccatttc tgcgacattg aggtacatct ccaggatgcg
     1741 ttttttcccc cagatcaatt cgatcatcga cgtgaagtag acctccaggc ctttccggaa
     1801 ccagctgcgg ccctgccaaa gaaagacgtt cttggccacc tgctggctga tcgtgctggc
     1861 gccatgaagg gatttactct tttggttatg ttttaatgcc ttctcgatat ttttgaaatc
     1921 gaacccgtcg tggtcaggaa aaagctggtc ttcggtactt atgaccgcaa gccttgcatt
     1981 gggggaaatc tcttttctgc cgacataggt ccgtttaagc ccatcgccgc gaaggaggct
     2041 gccgatctcg gtaatcgtga tgggcgggta gacccactta agcagcacga tgtataacag
     2101 ctgggcgacg aacaggataa tcagaacgcg acggctcacc ctccagatgc gggaaagcag
     2161 tcctcttttc cttttattcg gggttgtcac tttgtcaatt gcaaataagt cagcttgaac
     2221 cgtccgccca gtttataata tttaggcgca aattctgcta acaggaaccc tccggcgagc
     2281 aggacgccgc
//
LOCUS       DATGKE010000640         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_837645, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000640 DATGKE010000000
VERSION     DATGKE010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837645"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VKU83_10805"
     CDS             <1..371
                     /locus_tag="VKU83_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HLI94094.1"
                     /translation="AITAYLLSETIFYIATLFFASEIFDEPRSSKRSILLLFLNYIEI
                     TFDFAVIYGGLNLLQGKAHSRLDFVYFSFMTSSTIGFGDIYPTTPLGKYVVILHSIIF
                     LFFIVLFLNFFTRVDTRIHK"
     gene            complement(405..1859)
                     /locus_tag="VKU83_10810"
     CDS             complement(405..1859)
                     /locus_tag="VKU83_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLI94095.1"
                     /translation="MANVDVCKSFSSYDYYRALFETPQRNCVLLLDAGGFILEVNRAF
                     VSSFGYDADDLIGEHFSMLFTAEDRAKDKPMREIHTVLDEGQSFDNNFLVNKNNVRTW
                     VSGESVLLSNTQGQKCILKIIQNIHTQKESEYSIVSLNNFNENILSTIDDAVLVLDQE
                     LQIVKANRSFYRLLDQSDASLPGAGMRKLPESFEIKGELSAAVAAALGTGNCKPPFEF
                     EFEDTGGVKRAFEVSCSRLDSQTGKPNALIVFHDITIQRNQERQREDILNFVAHELRN
                     PLTNIVLNIGWMERQMTEESLGGYKEFLERAARNAERLKKLVNELYESTRLIAGNYDL
                     RTTPCIVADCIFECVEAVQHEHPDFYIEQQTGSQVKVLADKDKVTQVLNNYLSNAVKY
                     SGENKHITVGTAIADGYVTVSVTDRGKGIPPKDLPFIFNRFYRAAKTRSLEGLGVGLF
                     LCRQIIEAHHGKTWAESIEGKGSTFYFSLPVAAN"
     gene            complement(2159..2623)
                     /gene="arsN2"
                     /locus_tag="VKU83_10815"
     CDS             complement(2159..2623)
                     /gene="arsN2"
                     /locus_tag="VKU83_10815"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HLI94096.1"
                     /translation="MLQKSAEKMEIQRGTDYRPGITELLVASKLPADDLPASLDQFVV
                     VLHEESVIGVAGIEPYEKYGLLRSVAVQPSYQNKGIAAALLKHAESLAAANGIRTLFL
                     LTETAERYFARKGYETVNRDEVPEMLKQSSEFSHVCPKSALVMQKQLTGANP"
     gene            complement(2632..3054)
                     /locus_tag="VKU83_10820"
     CDS             complement(2632..3054)
                     /locus_tag="VKU83_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI94097.1"
                     /translation="MDIKIVKTETDISKTWEVMHLLRPHLVAGEYVNLVMEMLSKGYN
                     MAYIEEDGKAVSAVGFRELLFLYNGRHIYIDDLSTLDSHRGKGYGGLLVDYVKNLAKE
                     KGYKQVTLDSGYGRHAAHRLYMNKGFITSAHHFSVDVK"
     gene            complement(3116..>3701)
                     /locus_tag="VKU83_10825"
     CDS             complement(3116..>3701)
                     /locus_tag="VKU83_10825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HLI94098.1"
                     /translation="EVIINGISREVEKVIINPGYKKTPADLEQGDAAPLMAFKSRVDD
                     IALIKLKRPVIDVLPASLYKGRSEKGQIAEIIGCGATGNGLTGQDAGSPHRGKLRRAY
                     SRVVDTTDRWILLRFDAPPMALPMQGSPSDGDSGSPVLINVKGRPRLAGLVSGKYATG
                     KLADFHFSLYGQITFQVRLSRYIDWIDMTTGIKI"
BASE COUNT          885 a          973 c          839 g         1004 t
ORIGIN      
        1 aggcgataac ggcctacctg ctgtcggaaa caatctttta tattgcaacg ctgttctttg
       61 cctccgagat tttcgatgaa ccccgatcgt ccaagcggtc gatcctgtta ctgttcctca
      121 actacatcga gattaccttc gattttgccg tcatatacgg aggactcaac cttctgcagg
      181 gaaaggctca ttcccggctc gactttgtct atttcagctt catgacctcg tcgacgatcg
      241 ggttcggcga tatctatcca acgacgcctc tggggaaata cgtggtcata cttcattcga
      301 tcatattcct tttcttcatc gtgctcttcc tgaatttttt caccagggtc gacacgagga
      361 ttcataagta atgatcgcca cagcgggcgc cctacccatc atatttaatt cgccgctacc
      421 ggtaatgaaa agtaaaaagt gctccccttt ccttcgatac tctccgccca tgtcttgcca
      481 tgatgggcct cgatgatctg ccggcagaga aacaggccga caccgaggcc ttcgaggctc
      541 cgggtctttg cagcccggta aaagcgatta aaaatgaacg gcaaatcctt cggtggaatg
      601 ccctttcccc gatccgtgac cgatacggta acatatccgt cagcgatggc ggtgcccacc
      661 gtaatatgct tgttttcccc cgagtatttt acggcattgg acagatagtt gttcagcacc
      721 tgcgtgacct tgtccttgtc tgccaagacc ttcacctggc taccggtttg ttgctcgatg
      781 taaaaatccg gatgctcatg ttgcaccgct tctacacatt cgaaaatgca gtcggcgaca
      841 atgcaggggg tagtccgaag gtcatagttt cccgctataa gcctggtaga ttcatagagt
      901 tcgttgacga gctttttaag acgttccgcg tttcgcgccg cccgctcgag aaattccttg
      961 tagccgccca ggctttcctc ggtcatttgt cgttccatcc agccgatatt cagtacgata
     1021 ttcgtgaggg ggtttcgcag ttcatgcgcc acgaagttca ggatgtcctc ccgttggcgc
     1081 tcctggttcc tttggatggt gatgtcatga aacacgatca atgcgttagg cttccccgtt
     1141 tggctatcaa gccgcgaaca gctaacttcg aacgcgcgct ttacgccgcc ggtgtcctcg
     1201 aactcgaact cgaatggcgg cttacaatta ccggttccca gggccgcagc gacggcggcg
     1261 ctaagctcgc cttttatctc aaagctttcc gggagcttcc gcatcccggc gccgggcagc
     1321 gaagcatccg actgatccaa cagccgataa aaagaccggt tggccttgac gatctgaagt
     1381 tcctggtcca aaacgagcac ggcatcgtcg atagtcgaca ggatattttc gttaaaattg
     1441 tttaagctga caatggaata ttcagactcc ttttgggtgt gaatattctg aatgatcttc
     1501 agaatacatt tttgaccctg ggtattgctc aacagcacgg attcaccgga tacccaggta
     1561 cgaacattat ttttattgac caggaagttg ttatcgaatg attgtccctc atcgaggaca
     1621 gtatggatct cacgcatggg tttgtccttc gcccggtctt ccgcagtgaa aagcatggaa
     1681 aaatgttcgc cgatcaaatc atccgcatca tagccgaaag acgagacaaa tgcccgattg
     1741 acctcgagga taaaaccgcc ggcatccagg agcaggacac aattccgttg tggagtttca
     1801 aagagcgccc gataataatc gtaagaggaa aaacttttac aaacgtctac atttgccatg
     1861 cgagaacttt agtaggcgaa tgtaagaaaa atagtaaaat agtggccagc ctggaaaggc
     1921 cggccgtagc aatatcgcac agtccgcgcg cgtttgccat tcaaaacatc ttgaagcccg
     1981 ggccaattgt gggtatgtta tttctgttgc ttctgttgca ttaatagatt tagaatccaa
     2041 atctatcgca gaacgcatcc ggatacgacg tggtgcggtg ccgtcaatct agcacggcaa
     2101 ctggatgccg tattgcaacg gacgataatg ttctatcctt gaaaaaaagc ggacatgctt
     2161 atgggttggc tcctgttaat tgcttttgca ttaccagggc tgacttaggg caaacatgac
     2221 tgaattcgga tgattgtttc agcatttcgg ggacttcatc tctgttaacg gtttcatagc
     2281 ccttgcgagc gaaataacgt tccgctgtct cggttagcag aaagagcgtc cggataccgt
     2341 tagctgccgc aagggactct gcatgtttca gtagcgctgc tgcaatgccc ttattttgat
     2401 aagatggttg cacagccacc gaacgtaaaa gtccatactt ttcatatggt tcgatgcctg
     2461 caaccccaat aacactttct tcatgtaaga ccacgacaaa ttgatcgagg cttgccggca
     2521 aatcatcagc gggtaacttg ctcgctacca gcaactctgt gatgcctggc ctgtaatccg
     2581 ttccacgttg aatttccatt ttttcagctg atttttggag cacataacca tttatttcac
     2641 gtcaactgaa aaatggtgag cggaggttat gaagcccttg ttcatgtata atctgtgagc
     2701 ggcgtgtcta ccataacccg agtccagagt gacctgttta tatcctttct cctttgccag
     2761 gttctttacg taatctacta atagcccccc atatcccttc ccccggtggc tgtccaatgt
     2821 cgacaagtca tcaatataaa tgtgccgccc gttataaaga aaaagcaatt ctctgaaccc
     2881 aacggccgac accgctttgc catcttcctc tatgtatgcc atattgtatc ctttggaaag
     2941 catttccatt acgagattga catattcgcc ggcgaccaaa tgtggccgca gcagatgcat
     3001 cacttcccaa gtcttggaaa tatcagtctc tgttttgaca atttttatat ccatttcagt
     3061 ttttcttcat agatatttat caatgtgcta tttcgccaca acgccccgga tagcttcaaa
     3121 tttttatccc ggtcgtcatg tctatccaat caatgtagcg cgacaacctg acctggaagg
     3181 ttatttggcc ataaagcgaa aaatgaaagt cagcaagctt tccggtcgca tatttccccg
     3241 atacaagacc ggcaagtctg ggccggcctt tcacattgat caaaacagga cttccgctgt
     3301 cgccgtcact tggcgaaccc tgcatgggaa gcgccattgg aggggcatca aatcgcaaca
     3361 gtatccaccg gtcggtcgta tcaacgaccc tgctataggc gcgccgtaat ttgccgcgat
     3421 gaggagaacc ggcatcctgg ccggtaaggc cattgcctgt ggcgccacat cctataatct
     3481 ctgcgatctg ccccttttcg ctccttcctt tgtatagcga agccggcaat acatcaatta
     3541 ccggccgttt caattttatt agtgcaatat catcgacccg ggacttaaag gccatcagtg
     3601 gcgcagcatc cccctgctcc aaatccgcgg gtgtcttttt gtagcccggg ttaatgatga
     3661 ccttctccac ttccctggat atgccattaa tgatcacttc a
//
LOCUS       DATGKE010000641         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_661242, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000641 DATGKE010000000
VERSION     DATGKE010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661242"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VKU83_10830"
     CDS             <1..1221
                     /locus_tag="VKU83_10830"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI94099.1"
                     /translation="EPLGRSSYLGIDYGVQINNTHSDQNSYNKSQDGKYDLLDSPFSN
                     NYQYDVLAHRGGLAYTYVSRKLRLGFGNDIAFSSYSQRDLVVDTSIRRHFINWYPNAS
                     LSYSFASETRLFFWYNGYTSPPSLQQLQPIASNENPLNVVIGNPGLKPAFGNQFNLFY
                     NSYQVFSESSFNADVSYSFTSNAISNSSTVNDTTGKTITQAVNTNGNYRWYANLGYGF
                     KWKRPDLHFNVNFGANRSNNVTVVNDFINATQSATYTGGMGIWKSKEKKYDLSLNGSV
                     SYTTSKSSDDPALVTQYFTYSIQPGVDVYLPWHIQVHGDASLNIRQKTPVFNTNNDVY
                     LVNGWIGKKMLKNDQLVFKAAVNDLLDQNNGFDRNVSSSFISQNTYSTIKRYFMFSIV
                     WNFTKAGLPAPNRN"
     gene            1232..2005
                     /locus_tag="VKU83_10835"
     CDS             1232..2005
                     /locus_tag="VKU83_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HLI94100.1"
                     /translation="MNRIAPALPALLLIAAMTGTASAQNTIFLSQGKIEYERRINQYA
                     QMEERRDPGDNSGWAEISKKFVGDKFKTDYFNLLFTREKTLYKPGKESPGRPNPFFND
                     PPAQENVVYADLGQGKEVGQKTVFGHIFLLRDTLRRIHWKITDEMRVIAGFNCRRANA
                     IIMDSIYVVAFYTDEILTSGGPESFSGLPGMILGVALPHEHITWFATRVEAVPVAPTE
                     LIPPIKGNKVNNKELLETINSTFKDWGREGAFYLREIML"
     gene            complement(2009..>2376)
                     /locus_tag="VKU83_10840"
     CDS             complement(2009..>2376)
                     /locus_tag="VKU83_10840"
                     /inference="COORDINATES: protein motif:HMM:NF021922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HLI94101.1"
                     /translation="WPGDTKSGFTGEGLTQEQLAVQQLVKQLGNFRLHSSALRTGKLM
                     QYTPQGGLYVYFRYDDRQTVLCAMNNGTKPLKLDFSRFSERTAGFTRGVDVLNGESHA
                     LDQPAEIPARTMWVLELRK"
BASE COUNT          607 a          629 c          613 g          527 t
ORIGIN      
        1 gaaccgctag ggaggagttc ctacctgggc attgactacg gggtccagat caacaacacc
       61 cattctgacc agaactcgta taataagtca caggatggca aatacgattt gctggacagc
      121 ccgttcagca acaattacca atacgatgta ctggcgcacc ggggaggcct cgcctatacc
      181 tatgtttcca ggaagctgcg gctgggattt ggcaacgata tcgccttttc gagctacagc
      241 cagcgtgacc tggtggtgga cacgtccatc cggcgacatt tcattaattg gtatccgaat
      301 gcgtcgttat cttatagttt cgcctcggaa acgcggctgt ttttctggta caacggttat
      361 acatcgccgc catcccttca gcaactgcaa cctatagcga gtaatgaaaa ccccctgaac
      421 gtcgtcatcg ggaacccggg gttgaagcct gcattcggca accagttcaa tctgttctat
      481 aacagctacc aggttttttc ggagagcagc ttcaatgctg acgtcagcta ttcattcacc
      541 agcaatgcca tcagtaacag ttcgaccgtc aacgatacga ccggtaaaac gatcacccag
      601 gcggtcaaca ccaacggaaa ttaccggtgg tacgccaatt tgggctatgg cttcaaatgg
      661 aagcggcccg atctgcattt caatgtcaat ttcggcgcca acaggagcaa taatgttacg
      721 gtggtcaatg atttcatcaa tgctacccaa tcggccacct atacgggagg aatgggaatc
      781 tggaaatcca aggagaagaa atatgatctc tcactgaatg gaagtgtaag ttacacgacg
      841 agtaagtctt ccgacgatcc ggccctcgtc acccagtact ttacgtatag cattcagccg
      901 ggcgtcgacg tctatctgcc ctggcacatc caggtccacg gtgacgccag cctgaatatc
      961 cggcaaaaga ccccggtgtt caataccaat aacgatgttt acctggtgaa tggatggatc
     1021 gggaaaaaaa tgctaaagaa cgaccaactg gttttcaaag cagccgtaaa cgacctgctg
     1081 gatcagaata acgggtttga ccggaatgta agcagcagtt ttatttcgca gaacacctac
     1141 agcacgataa agcgatattt tatgttcagt atcgtatgga atttcaccaa ggcgggtctc
     1201 cctgccccaa accggaatta aacagatccg tatgaataga atcgcccccg ctcttcccgc
     1261 tctcctgtta atagccgcga tgaccggaac cgcttcggcg cagaatacga tcttcctttc
     1321 ccagggaaag atcgaatatg agcggaggat caaccagtat gcgcagatgg aagaaaggcg
     1381 ggaccccggc gacaacagtg gctgggcgga aatctccaaa aagttcgttg gggacaagtt
     1441 taagaccgac tatttcaacc tgctgttcac gcgggaaaaa acgctttaca agccgggaaa
     1501 ggagagcccc ggacggccga atccattctt caacgatccg ccggcgcagg agaacgtcgt
     1561 ctatgcggac ctgggtcagg ggaaagaggt ggggcagaaa actgtttttg gtcatatatt
     1621 tttactcagg gacaccctgc gccgcatcca ttggaagatc accgatgaaa tgcgggtaat
     1681 cgccgggttc aactgccgcc gtgccaatgc gatcatcatg gattcgatct atgtcgtcgc
     1741 tttttatacc gacgagatcc tgacgtccgg aggacccgag agttttagcg ggctgccggg
     1801 aatgatcctg ggcgtggccc ttccgcacga acatattacc tggtttgcca ccagggtcga
     1861 ggcggtcccg gtggcgccga cggaattaat tccgcccatt aagggaaata aggtcaataa
     1921 caaggaattg ctcgaaacga tcaacagtac attcaaggac tggggccggg aaggcgcatt
     1981 ttacctgcgc gagatcatgt tgtaagcact atttccgcag ctccagcacc cacatcgtcc
     2041 tggccggtat ttcggccggc tggtcgagag catgcgactc gccgttcaga acatccacgc
     2101 cgcgggtgaa cccggcggtc cgctcagaaa atcggctaaa gtcgagtttc aacggcttcg
     2161 taccattatt catggcgcag agaacggtct ggcggtcatc atagcggaaa tatacataga
     2221 ggccgccttg cggcgtatac tgcatcaact tacctgtccg gagggcgctg gaatgcagcc
     2281 ggaaattccc cagctgtttt acgagctgct gaaccgccag ttgttcctgc gttaagccct
     2341 cgcctgtaaa tccgcttttc gtgtcgccgg gccaac
//
LOCUS       DATGKE010000642         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1158936, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000642 DATGKE010000000
VERSION     DATGKE010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158936"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VKU83_10845"
     CDS             <1..195
                     /locus_tag="VKU83_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HLI94102.1"
                     /translation="QYILHRFGISLADAVAVGDGESDICMVKMAGTGISFNSTTPVLD
                     QVADHVFRDRSLQPVLAVAR"
     gene            269..2182
                     /gene="dnaK"
                     /locus_tag="VKU83_10850"
     CDS             269..2182
                     /gene="dnaK"
                     /locus_tag="VKU83_10850"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLI94103.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIANDEGRRTTPSIVAFLK
                     NGERKVGDPAKRQAITNPVNTIMSVKRFMGRRFDEVTNEITHWSYKVVKGDNNTVRID
                     IDGRLYTPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LNVRRIVNEPTAAALAYGLDKAGKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDKVVMDWLADEFKKDENVDLRKDPMALQRLKEAAEKAKIELSSSSETEIN
                     LPYITAVDGVPKHLVKKLSRAKFEQLSDRLFERCLHPCEQALKDAGISASQIDEVILV
                     GGSTRIPKVQEIVEKFFGKKPNKGVNPDEVVAVGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIESNTTIPTKKSETFSTASDNQPGVQIHVLQGERPMASQNKSLGT
                     FNLDGIPPAPRGVPQIEVTFDIDANGILHVTAKDKGTGKEQKIRIEAGSGLSKEEVER
                     MKSEARANEATDKAAREKVDKINQADSMIFQTEKQLKEYGDKLPADKKAPIEAALNRL
                     KAAHKAQDIAGIDASIAEMNNAWTAASEELYKATQQAGGAQGGPQGGPGNGAQQPNAG
                     SENVTDAEFEEVK"
BASE COUNT          570 a          577 c          654 g          400 t
ORIGIN      
        1 caatatattc tgcaccgttt tggcatcagc ctggcagacg ctgtggcagt cggggacggg
       61 gaaagcgata tttgtatggt caaaatggcg ggaaccggga tttctttcaa ttccaccacg
      121 ccggttctcg accaggtggc agatcatgtg ttccgcgaca ggtcgcttca gccagtcctg
      181 gccgtagcca ggtgacaaat ctgccatttt tgcatttttc cctgcgtggc ataatgattg
      241 tcaatgcata tcagataaat ctattaatat gggaaaaata atcggaattg accttggcac
      301 cacgaacagt tgcgttgccg taatggaggg caatgagccc gtcgtcatcg ccaatgacga
      361 gggacggagg acaacccctt ccatcgtggc attccttaag aacggggaaa gaaaggtcgg
      421 ggatcctgcc aagcggcagg ccataacgaa tcccgtgaac acgatcatgt cggtgaaacg
      481 ctttatgggt cgccgttttg acgaggtgac caacgagatc acccactgga gctataaggt
      541 cgtcaaagga gacaataata ccgtgcggat cgatattgac ggccggctat ataccccgca
      601 ggagatttcc gcaatgatcc tgcagaagat gaagaaaaca gccgaagatt atctcggcca
      661 ggaagtgacg gaggccgtca ttaccgtgcc ggcgtatttt aacgatgccc agcgccaggc
      721 taccaaggaa gcgggtgaaa tcgccggcct gaacgtccgg cgcatcgtca acgagccgac
      781 tgcggccgct ctggcttatg gtcttgataa agccgggaaa gacgagaaga tcgccgtgtt
      841 tgacctcggg ggcggaacct tcgatatctc catcctcgag ctgggcgatg gcgtatttga
      901 agtgaagtcg accaacgggg acacccacct cggcggggac gacttcgata aggttgtgat
      961 ggactggctg gccgacgaat ttaaaaaaga tgaaaatgtt gacctgcgca aggatccgat
     1021 ggcccttcaa aggctgaagg aggctgcgga gaaggccaag atcgaactgt cctcttccag
     1081 cgaaacggag atcaacctgc cttatatcac ggccgttgac ggcgtgccga aacacctggt
     1141 taagaagctt tcccgggcga aattcgagca gttgtccgac cggttgttcg agcgctgtct
     1201 gcatccctgc gagcaggccc tcaaggatgc cgggatcagc gcctcccaga ttgacgaagt
     1261 gatcctggtt ggggggtcca cccggatccc gaaggtccag gagatcgtcg agaaattctt
     1321 tggaaagaag cccaataagg gcgttaaccc cgatgaagtc gtcgcagtcg gcgccgctat
     1381 ccagggtgcg gtgctcaccg gggaagtaaa ggacgtgttg ttgctggacg ttacacctct
     1441 ctccctcggg atcgagacga tgggcggggt gatgacccgg ctgatcgagt ccaacaccac
     1501 cattccgacg aaaaagtcgg aaaccttttc caccgccagc gacaaccaac ctggcgtaca
     1561 gatccatgtc ctgcaaggtg agcgcccaat ggccagccag aacaagagcc tgggaacctt
     1621 caacctggat ggcatacccc cggcgccccg cggcgtgccg cagatcgaag tcaccttcga
     1681 tatcgacgcg aacggaattc ttcatgtaac ggcgaaggac aagggcaccg gaaaggaaca
     1741 gaagatccgg atcgaagccg gtagcggtct cagcaaagag gaagtggagc gcatgaagtc
     1801 cgaagcaagg gccaacgagg cgaccgacaa ggctgcccgt gagaaggtcg acaagatcaa
     1861 ccaggctgac agcatgatct ttcagaccga aaagcaactg aaggaatacg gcgataagct
     1921 tcccgccgac aaaaaggcgc cgattgaagc ggctttgaac aggctgaagg cggcccacaa
     1981 ggcacaggat atcgccggta tcgacgcatc gatcgccgaa atgaacaacg cctggacggc
     2041 agccagcgaa gaactgtaca aggctacaca gcaggcgggc ggcgcacaag gtggtcccca
     2101 gggcggacct ggtaacggcg cccagcagcc gaatgccggc agcgagaacg ttacggacgc
     2161 agaattcgag gaagtgaagt aagcaaatat atgttttgac g
//
LOCUS       DATGKE010000643         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_558106, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000643 DATGKE010000000
VERSION     DATGKE010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558106"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VKU83_10855"
     CDS             complement(<1..240)
                     /locus_tag="VKU83_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94104.1"
                     /translation="MHFDARPIFKMPPVLWVSALLAVYISGCDKANCGTTTSALFNSA
                     DKQFMDQESQFNSDEAAAATLALGAGGMGGRAFAQS"
     gene            373..2745
                     /locus_tag="VKU83_10860"
     CDS             373..2745
                     /locus_tag="VKU83_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLI94105.1"
                     /translation="MTKSMLLRRAVYQFLSIANLLPFAAAAQSGPAPQLGKDPIPVII
                     KAMTLEEKAKLVVGNGFRMPRANNGPVVGRTQAKVPGAAGTTAAIPRLGIPSIVVSDG
                     PAGVRIDSVRQDAPGQTFYATAWPVGTLLASSWDTALVQRVGSSFGNEAKEYGIDIML
                     APALNIHRNPLGGRNFEYYSEDPLIAGDMTAAIVRGIQSNGVGTSIKHFAGNNQETNR
                     NTVNTIVSERALREIYLKGFEIAVKKTQPWTVMSSYNLINGTYTSQEYDLLTTILRKE
                     WGFKGFVMTDWFGGSDPVAQMNAGNNLLMPGTPEQSQKIIDAVKSGAIAEKMLDENVA
                     GILKVILKSPAFAKYKYSNKPDLHRHAQLSREAAAEGMVLLKNDDRSLPLGKSVRNVA
                     LFGINGYDLIAGGTGSGDVNKAYKISLAQGLANAGYSVDQDLKETYTSYLAAYAASHP
                     KKNFFAEFMNPTPPAPEYLVDKDLLTKKVATADVAILSIGRNAGEGRDRKLADDYYLS
                     DTEKVVIGQIAEAFHAQHKKLIVVLNIGGVIDVMQWRDQPDAILLAWQPGLEGGNAIT
                     DVLKGKVNPSGRLATTFPARYEDVPSAKNFPGKEFPEKATTGSFGRKQIPAEVVYEEG
                     IYVGYRYFNTFHVKPAYEFGFGLSYTRFRFSNLKLSSKTFNGSLMASVTVTNTGDVPG
                     KEVVQLYLTAPAQKEDKPAEELKAFGKTRLLGPGQSQTLTFTLTAADLASFDTPSASW
                     VAEAGTYTVRIGASSEDIKQTGSFSLPGDVMVEKDHNVLVPLVNIDELKK"
     gene            complement(2749..4161)
                     /locus_tag="VKU83_10865"
     CDS             complement(2749..4161)
                     /locus_tag="VKU83_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLI94106.1"
                     /translation="MANKVEDLAAGAVDGLASQVRGNVILPRHGTYEQARKVYNAMID
                     RRPGMIIKCCDVADVIAAVKFASRQGLLTAVRGGGHNGAGLGVCDDGMVIDLSGIRYV
                     HVDQATHMVRVGGGTTLGEIDHATHVFGLAVPAGIISTTGIGGLALGGGIGYLSRKYG
                     LTIDNLIEAEIVLADGNVMVANEERNADLFWAIRGGGGNFGIVTSFKFQAHPVREVFG
                     GVTLWPVEMTAEIMEWYDDFIQTSPNELNGFIAVLTVPGPPFPAPLHNKQFCGIVWCY
                     AGDLVNADELFSPVRAKCPVFEHVGPMPYPAIQRMFDGLFPPGLQWYWRADFFDSLGP
                     QARQVHEEFGKRIPTPLSQMHLYPISGAVGNIAKDATPWAYRHAKYAGVILGVDPDPA
                     NAAKLTAWCKSYWEALHPYSCGGAYVNFMMSEGPERVRSSYGSNYDRLAQIKRKYDPT
                     NLFRVNQNILPATETAAVSG"
     gene            complement(4262..4630)
                     /locus_tag="VKU83_10870"
     CDS             complement(4262..4630)
                     /locus_tag="VKU83_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLI94107.1"
                     /translation="MKLMHSLTPAEERFLTDLMDFTESAWRDFNVKVDDFGKPVGCSK
                     SQLYRKMISLTGKSPNTFLKEYRLNEALELLNKNARNVSEVAYETGFNSPSYFCKCFH
                     KHYGRLPSDFLPLAGHRAGV"
BASE COUNT         1098 a         1280 c         1296 g         1011 t
ORIGIN      
        1 gctctgcgcg aaagccctgc cgcccatccc gccggcgcct aacgccaggg tcgcagcggc
       61 agcttcgtcg ctgttaaatt ggctttcctg atccataaat tgtttgtccg cactgttgaa
      121 taacgcggat gtcgtcgtcc cgcagttagc cttatcgcag ccggagatgt agactgcgag
      181 caacgcggat acccacaaaa ctggaggcat tttgaagatt gggcgggcat cgaagtgcat
      241 gaggcgaggt tttggatcat atatgcaaaa gccattctat gggaagggcg aggcaacaaa
      301 caaaaaatac cggaagatta atgtatcatt ctaatacaat gaccttattt ttgtgacgac
      361 caactaattt ttatgactaa gtcaatgctt ctgcgccggg ctgtttatca gtttctaagc
      421 atcgcaaacc ttctgccatt tgcagcggcg gcacagagtg gccctgcgcc tcaacttggc
      481 aaggatccca ttcccgttat tatcaaagcg atgaccctcg aggagaaggc caaactggta
      541 gtcggcaacg gcttccgcat gccgcgggcc aacaatggtc cggtcgtcgg gcgcacgcag
      601 gctaaagtgc cgggtgccgc cggaactacg gccgccattc cacggctggg tattccttcg
      661 atcgtcgtca gtgacgggcc cgcgggagtg cggatcgatt ccgtcaggca ggatgcgccg
      721 ggccaaacgt tctacgcgac tgcctggccg gtcgggaccc tcctggcctc cagctgggat
      781 acggctctgg tacaacgcgt cggcagttcg ttcggaaatg aggcaaaaga gtatggcatc
      841 gatatcatgc tggcccctgc cttaaacatt caccgcaatc cgctgggggg ccgcaacttt
      901 gaatactatt cggaggaccc cctgattgca ggcgacatga cggcggccat tgtgaggggt
      961 atccagtcga atggcgtcgg gacctcgatc aaacatttcg cgggcaataa ccaggagacc
     1021 aaccggaaca ccgtgaacac gatcgtcagc gagcgggcac ttagggagat ctatttgaaa
     1081 ggatttgaga tcgcggttaa gaagacgcag ccctggacag taatgagctc ctataacctt
     1141 atcaacggca cctacacctc gcaggaatac gatctcctga ccaccatcct gcgaaaggaa
     1201 tggggattca agggatttgt catgaccgac tggtttggcg gctcagaccc tgtagctcaa
     1261 atgaatgccg gaaataatct gttgatgcca ggaacaccgg aacagtcgca aaagatcatc
     1321 gacgcggtga aaagcggggc catcgccgaa aaaatgctgg acgaaaatgt cgcgggcatc
     1381 cttaaggtca tcctcaaatc ccctgctttc gcaaaatata aatattccaa caagcctgac
     1441 cttcatcgcc acgcgcaact gtcgcgggaa gcggctgctg agggaatggt actgctgaag
     1501 aacgacgacc gcagcctgcc gttgggcaaa tcggtaagga acgtggcctt attcgggatc
     1561 aacgggtatg atctgatcgc cggcggtacc ggaagcggcg acgtcaacaa ggcctataag
     1621 atctcgctgg cgcagggcct ggccaacgcc ggttattcgg tcgaccagga tctgaaggaa
     1681 acctacacgt cctacctggc cgcctatgcc gcgagtcacc caaagaaaaa ttttttcgcc
     1741 gaattcatga accccacgcc accggcccct gaataccttg tcgataagga cctgctgacg
     1801 aagaaagtgg ctaccgcgga tgtggcgatc ctgtccatcg gacgaaatgc aggcgagggg
     1861 cgggaccgga agctggccga tgactattac ctttccgaca cggaaaaggt ggtgatcggc
     1921 cagatcgcgg aagcttttca tgcgcagcat aagaaactca tcgtcgtgct gaatatcggc
     1981 ggggtcatcg atgtcatgca gtggcgggac cagcctgacg cgatactgct tgcctggcag
     2041 ccgggtcttg aaggcggcaa tgccatcacc gacgtactta agggtaaagt gaacccgtct
     2101 ggcaggctgg cgacaacgtt ccctgcaaga tatgaggatg tcccgtccgc gaagaacttt
     2161 ccgggaaaag aattccctga gaaagcgaca accggaagct tcgggaggaa acagatccca
     2221 gccgaggtgg tctacgagga agggatctac gtcgggtacc gctactttaa tactttccat
     2281 gtcaagcccg catatgagtt tgggttcggg ctctcgtaca cccgttttcg cttttcgaat
     2341 ctgaaattga gcagtaaaac tttcaacggc agccttatgg cgtcggtgac ggtcaccaat
     2401 acgggagatg tcccgggcaa ggaggtggtg cagctctatc tcacggcgcc cgcgcagaag
     2461 gaggacaagc ctgccgaaga attgaaggcg ttcggcaaga cgcgtttgct cgggccaggc
     2521 caatcccaaa ccctgacctt caccctgacg gccgcagacc tggcgtcgtt tgacaccccg
     2581 tcggcctcct gggtcgcgga ggccgggacg tacaccgtca ggatcggggc ctcgtcggaa
     2641 gatattaagc agaccggttc attcagcctt ccgggcgacg tgatggtgga gaaggatcac
     2701 aacgtccttg tcccgctcgt gaacatcgac gagttgaaaa aataaggatt aaccggaaac
     2761 ggctgcagtt tccgttgccg gcagaatgtt ctggttgacc cggaataaat tggtggggtc
     2821 atacttccgc ttgatttgtg caagccggtc ataattactt ccatagctcg atctcacccg
     2881 ttccggaccc tcgctcatca tgaagttgac gtatgcgccg ccgcatgaat acggatgaag
     2941 tgcttcccag tagctcttgc accatgccgt cagcttagcg gcgttggccg gatcgggatc
     3001 gacacccaaa atcacgccgg cgtacttggc atggcggtat gcccatggcg tggcatcctt
     3061 tgcaatgttc ccgaccgcgc cgctgatcgg ataaaggtgc atctgagata agggggtggg
     3121 aattcttttt ccaaactcct catgtacttg tcgtgcttgc ggtccgagcg agtcgaagaa
     3181 gtcggctcgc caataccatt gtagacctgg cgggaacagt ccgtcgaaca ttcgctgaat
     3241 ggcaggatag ggcatcggcc cgacgtgttc aaagacgggg catttggcac gcaccgggct
     3301 gaacaattcg tctgcattaa ccaggtctcc cgcgtagcac cagacgatgc cgcagaattg
     3361 tttgttatgc aggggggccg ggaacggcgg ccccggcacg gtgagcacgg caatgaatcc
     3421 gttcagctca ttcggcgacg tttgaataaa atcatcgtac cattccatta tctccgcggt
     3481 catttcgacc ggccacaatg ttacgccgcc aaacacctcc cggacgggat gcgcctgaaa
     3541 tttgaaggac gtcacaatgc cgaaattgcc cccgccgccg cgaatggccc aaaaaagatc
     3601 ggcgttcctt tcttcgtttg cgaccatgac gttaccgtcc gccagaacga tttccgcctc
     3661 gatcaggtta tcgattgtaa gcccatactt cctggagaga tagccaatgc ccccgccaag
     3721 tgccagaccg cctatgcccg ttgtcgagat aatgccagcc ggcacggcaa gaccgaaaac
     3781 atgggtagcg tggtcaattt cgccaagcgt cgtgccgcca cctacgcgga ccatatgcgt
     3841 cgcctgatcc acgtgtacat acctgattcc ggacaggtca attaccattc cgtcgtcaca
     3901 gacgccaagg ccggctccgt tatgtccgcc gccccgcacc gccgtgagca agccctgccg
     3961 gcttgcgaat ttgacggcgg caatgacatc cgccacgtcg caacatttga tgatcatgcc
     4021 gggccgacgg tcgatcattg cattgtaaac ttttcgggct tgctcgtatg ttccgtgacg
     4081 gggaagaatc acgtttcctc tgacctgcga agccaaaccg tcgacggctc cggcagcgag
     4141 gtcttcgacc ttatttgcca taaagaattt gtttgtgtcc gcttaatctt gtgtcgctcg
     4201 aagatagcta cgcgaatgcc gttcggctgt cacatttggt tcaaaaaatt gcaaatttgg
     4261 gtcaaacgcc tgcgcgatgg ccggccagag ggagaaaatc agatgggagg cggccataat
     4321 gtttatgaaa acatttgcaa aaataggacg gactgttgaa gccggtttca taagccactt
     4381 ctgagacatt cctggcattc ttattcaaca gttcaagcgc ctcattaagg cggtattcct
     4441 ttagaaacgt gttcggcgat ttgccggtta gagaaatcat ttttcgatat agctgggatt
     4501 tgctgcaacc cactggtttg ccgaagtcat caacttttac attgaaatcc cgccaggccg
     4561 attcggtaaa gtccattaag tcggtcagga accgttcttc cgcgggcgtg agagaatgca
     4621 tcagcttcat cttactgagc tgtcccggcc tttcgcttcg atacaattca gtgaccaggg
     4681 aggaa
//
LOCUS       DATGKE010000644         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_972017, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000644 DATGKE010000000
VERSION     DATGKE010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972017"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VKU83_10875"
     CDS             complement(<1..323)
                     /locus_tag="VKU83_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94108.1"
                     /translation="MAINRKVLTLDEFTLQQLRIFPNATGELSNLLRDIGLAAKRVNV
                     EVNKAGLVDILGDAGSVNVQGEDVKKLDVFANNQLMGVLRHGISCAGIGSEELADFVV
                     FNDEI"
     gene            482..1756
                     /locus_tag="VKU83_10880"
     CDS             482..1756
                     /locus_tag="VKU83_10880"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLI94109.1"
                     /translation="MNKIFKFGGASVNSVERIQRLPHILRAYPDDRLLVVVSAMGKTT
                     NALEKVAEAFSAGSRDEALRLFGQIKDAHLMTAKYLLVQNYLAAEAQLRDFFTEIEWL
                     LHDKPVRDYDYYYDQIVCVGELLSTSIVSAFLAESGIGNHWIDVRDIFRTDANFRDAA
                     IDWEFSQQQVREQVLPLFESERMVLTQGFIGATAENESTTLGREGSDYTAAVFAAMTG
                     ADSLTIWKDVEGVMNADPKQFSAAVIVPELNYDEVIEMAYYGAQVIHPKTIKPLQNKD
                     IPLYVKCFLDPALPGTVIHNQSLKGLPPIFVLKDNQVLLHLHSKDFSFVGETGAERLY
                     HLCATIRVKPNLIQTGAVTLQVCLDDRTDKIERLAFEAAEFFDVQVERGLTLLTIRHY
                     DSGSLEKWTKGREEVLRQQTRETVQVLMRQER"
     gene            complement(1832..>2398)
                     /locus_tag="VKU83_10885"
     CDS             complement(1832..>2398)
                     /locus_tag="VKU83_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94110.1"
                     /translation="LNKYLQTFSEYAPFVKSMRTAFNYFEKYREVPFVMVNGRGEYVT
                     DESAPSEALVKVPNLEPAAMAAAKKKPAHNRPERTDPIPDNQLATSADKLPEYPGGNR
                     AFQVFLNRVSKESVRDLAGQSKAFVMVEYVIDSTGKAVYAKAIRGGNDVMDEKIENAF
                     LGMPQWQPATQRGNHVAMKLKQTVMVGD"
BASE COUNT          516 a          687 c          660 g          535 t
ORIGIN      
        1 ctgatctcgt cattgaatac gacaaaatcc gccagctcct cgctgccgat gccggcacag
       61 cttatcccgt gcctcagcac tcccatcagc tgattgttcg caaatacatc cagtttcttc
      121 acgtcctcac cctggacgtt caccgaaccg gcgtcaccca ggatgtcgac caggccggcc
      181 ttgttgactt ccacattcac ccgtttggcg gcaagcccga tatccctgag gaggttgctc
      241 aactcgccgg tggcattcgg aaagatgcgg agctgctgca atgtgaattc atcaagggta
      301 agtactttcc ggttgatggc catatacgcg acttattttg gcaaaattat gtgagtcgca
      361 cgatatttag cgccttttag caattttggc cggatcgatc tgtcacaacg attgcccgtc
      421 cttatatttg cgccgtgaaa gccagagtat tcatgaaccc gatagaaaag acaggatgcg
      481 cgtgaataaa attttcaagt tcggcggcgc gagcgtgaac agcgtggaac ggatccagcg
      541 gctgccccac atcctccggg cctaccccga cgaccgcctg ctggtggtgg tatccgcaat
      601 gggcaagacg accaacgcgc tcgaaaaagt tgcggaagca ttttctgccg gttcgcgcga
      661 tgaggccctc cggttgttcg gccagatcaa ggacgctcat ttgatgaccg cgaaatacct
      721 gctggttcaa aattatctcg cagccgaggc acaactcagg gattttttta ccgagatcga
      781 atggctgtta cacgacaagc cggtgcgcga ctacgactat tattatgacc agatcgtctg
      841 cgtcggcgaa ctcctctcta cttcgatcgt cagcgccttt ctggccgaat ccggtatcgg
      901 caaccactgg atcgacgtgc gggatatatt ccggacggac gccaatttcc gcgacgccgc
      961 gatcgactgg gagttctcgc aacagcaggt ccgggagcag gtgctgccgc tcttcgaatc
     1021 ggaacgaatg gtgctgaccc agggttttat tggcgctacg gcggaaaacg aaagcaccac
     1081 actcggccgc gaaggcagtg actataccgc ggcagtattt gcggcgatga ccggcgcgga
     1141 cagcctgacc atttggaagg acgtagaggg cgtcatgaat gccgatccaa aacaattttc
     1201 agccgccgtt atcgtgcccg agctgaatta tgacgaggtc atagagatgg cctattatgg
     1261 cgcccaggtg atccacccga agaccatcaa gccgctgcag aataaggaca ttcccctgta
     1321 tgtaaagtgt tttctcgatc cggccttgcc cgggaccgtc atccataacc agagcttgaa
     1381 agggctgccg ccgatattcg tattgaagga caaccaggta ttgctccacc tccactcaaa
     1441 agatttttcc tttgtcggcg agaccggcgc cgaacggctg taccatctct gcgcgaccat
     1501 ccgggtcaaa cccaacctga tccagaccgg ggccgtgacc cttcaggtct gtctcgacga
     1561 ccggacggat aagatcgaaa ggctcgcgtt cgaggccgct gaatttttcg acgtacaggt
     1621 ggagcggggg ctgacgttgc tgacgatccg gcactacgat agtggatcgc tcgagaaatg
     1681 gacaaaagga agggaggagg tgctgaggca gcagacgagg gagacggtgc aggtgctgat
     1741 gaggcaggaa cgatgaaata gcttgtcgct ccacttcggc cgcccgcgcc ggctaaacgg
     1801 gcaatactct ttccggctac gggccgaccc gtcagtcccc taccatcacg gtttgtttca
     1861 gcttcatggc cacgtggttc ccgcgctggg tggcaggctg ccattgcggc atgcccaaaa
     1921 aagcgttctc gatcttttcg tccatcacgt cattcccgcc gcggatggcc tttgcataga
     1981 ccgccttgcc tgtggaatcg atcacatatt cgaccatcac aaaggccttg ctttggcctg
     2041 caaggtcgcg gacgctctct ttgctaaccc tgttcagaaa aacctggaag gctcggttgc
     2101 cgcccggata ctcgggcagc ttatccgccg atgtcgccag ctgattatcc gggatcggat
     2161 cggtccgctc gggccggtta tgggccggct ttttctttgc cgccgccatc gctgccggtt
     2221 ccaggttggg taccttcacg agggcctcgg acggggccga ctcatccgtt acgtattccc
     2281 ccctgccatt gaccatcacg aaggggactt cccggtactt ctcgaaataa ttgaaagccg
     2341 tccgcatgct ttttacgaac ggggcgtatt ccgaaaaggt ctggaggtat ttgttaag
//
LOCUS       DATGKE010000645         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1445225, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000645 DATGKE010000000
VERSION     DATGKE010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445225"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1172)
                     /locus_tag="VKU83_10890"
     CDS             complement(111..1172)
                     /locus_tag="VKU83_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLI94111.1"
                     /translation="MGYIFKILSAELLQFSGLSPLLDMYHKGDYSPLLTLSGTLDAIS
                     PVIPLLLLIELLRSLFYRRFKIEDYRMPFFVYVANRFLSRFISIAAIGFCLGLFSRIA
                     LLKSSFTWYWFLFGYIVWEFSHFIYHYLAHKVRLFWCLHSTHHAPEAMNLSVTYMHFF
                     LEAPYADVIRTSICILAGVNAPMLFVIMAIDGTWGAFIHVGENFIPDARMGIVGRLIL
                     TPSHHRVHHARNPLYIDTNFCNLLNVWDRIFRTYQPEDRSMPIQFGITRPVKTRSFWD
                     MYFGEFVCLARDVARAPGLLNKVLYLFMPPGWSHTGDHKTARVLRRQGILTVRQPQLP
                     VTCSDLAEQELDLAGKNSR"
     gene            1295..2524
                     /locus_tag="VKU83_10895"
     CDS             1295..2524
                     /locus_tag="VKU83_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI94112.1"
                     /translation="MTKGYSGQSSHDSGKPPKNNGTPSPRRTRVMVGVFFFVSGFGFA
                     TWASRIPTIQQELHLNEAQLGAVLFALPIGLLGTAPVTTVLLSRFDSRRIMIIGAIAF
                     NVMLCLIGFATRTWELVLALVCFGSSRNLMNISINAQSIGVQALFNRSIIARFHGVWS
                     LAGFAGAAVGSLMVSFSVPPLWHFAAVGFLLSILCWLALPGTVRQRPAPRERRPWFTL
                     PNRSLAKLGLISFASMACEGTMFDWSGIYFRKAVHAGQDVATLGFVAYMIAMTLGRLT
                     GDRLVNRLGIRTTLTYSGGLIGLGLLTAVCLPFPLTAGFGFILTGFGVSCVVPMVFGM
                     AGRTTGMSSGSAIASVSTVAYTGFLIIPPLVGSVAQLAGLRTAYTMIACFGFLITFLV
                     QFTLGEVQPLGGAAPLE"
     gene            complement(2704..3261)
                     /locus_tag="VKU83_10900"
     CDS             complement(2704..3261)
                     /locus_tag="VKU83_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94113.1"
                     /translation="MQYTPYTNLQAAPDFANFNFISPTLPGPIERQVRFISQPGGQIY
                     LVDFRNKPAESKEPPNWPESKDFLLVTCTLLQIVEIYTERYPQRVLRFRADSRLKRLI
                     FLNMALRFENLLSPLFLVDKNDGGLPRPEGDPVFVFQVQRKPVPFVAINTVQSTWNGT
                     SRIFGKPFKVEIDRSIRVGMTVPVI"
     gene            complement(3325..4098)
                     /locus_tag="VKU83_10905"
     CDS             complement(3325..4098)
                     /locus_tag="VKU83_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94114.1"
                     /translation="MNTGNLEFLNGRLKYLGFGDNTLLNQQLEDHIAREEPEFELTTD
                     AFFDHFIRIEAKLHFHRQDQQGIYVFDRYEAEIQYEQEPDRNKKRSFYIYKGIGVTFK
                     EAYNLLDGRAAKVDMINRDDEKYTVWVTLNFEERDMHGNYKFKYYRYFDLEKVLENYP
                     IVECGAPNTRKALLSALERGNRYLITLETTKPEPKWIEAHPAERTIRIYAPGPPKRRR
                     SEERRSTARRLSAASVEPAAEKPAGDQEVSEEPVGASGK"
BASE COUNT          918 a         1103 c         1121 g          986 t
ORIGIN      
        1 taaaagtgta ttgctctacc aactgagcta cgcaatcaac caatcaaaaa agaaaactgg
       61 tcttttttgg gagggcaaaa ataagtcaaa atgcacaggt cgccaatttt ttatcgacta
      121 tttttgcccg ccaaatccag ctcctgttcc gctagatcgc tgcaagttac cggcagctgc
      181 ggttgcctta ccgttaatat gccctgtcgt cgcaggaccc gggcggtttt gtggtcgccg
      241 gtgtgactcc atccaggcgg cataaagagg tacaagacct tgttgagcaa tcccggagcg
      301 cgggcgacat cccgcgccag gcaaacaaat tcgccaaaat acatgtccca aaagcttcgg
      361 gttttcaccg gccgggtaat tccaaattgg atcggcatcg agcggtcttc gggctggtac
      421 gtgcggaaga tcctatccca gacattcagg aggttgcaaa agtttgtgtc gatatagagc
      481 ggattgcggg catgatgaac ccggtgatgc gagggggtca ggatcagccg ccccacgata
      541 cccatccggg catccgggat gaaattctca cccacatgga tgaaagcgcc ccaggtaccg
      601 tcaatggcca tgatcacaaa caacatcggc gcgtttaccc ccgccaggat gcaaatcgag
      661 gttcggatca cgtcggcgta gggcgcctcg aggaaaaaat gcatataggt cacggacagg
      721 ttcatggctt ccggggcgtg atgggtggaa tgcaggcacc agaacagccg gaccttatgg
      781 gccaggtagt ggtatataaa atgcgaaaat tcccagacaa tatagccaaa taggaaccag
      841 taccaggtga agctggactt cagcagtgcg atcctgctga agaggccgag gcagaaaccg
      901 attgccgcga tgcttatgaa ccgcgagagg aaccggttgg ccacgtacac aaaaaatggc
      961 atccggtagt cttcgatctt gaaccgccga taaaacaggg agcgcagcag ctcgatcagc
     1021 aggagaaggg gaatgacagg cgatatggcg tccagcgtcc cggatagggt caacaaaggc
     1081 gaataatcgc ctttatggta catgtcgagc aagggactca aacctgaaaa ctgtaacagc
     1141 tcggccgata agatcttgaa gatataaccc atgggatagc caaatagatc gatgatccgg
     1201 ctgctaatat atcaataaat tctgctgcag tcaaacgacg gcgggaaaag gcgccggatc
     1261 cctatttttg cgatcttatt aagtgttaac agggatgaca aaaggttata gcggacagtc
     1321 gtcgcacgac agcggaaaac ctccaaaaaa taacgggacg ccgtcgccac gcaggacaag
     1381 ggtcatggtg ggggtctttt tctttgtttc cggcttcggc ttcgctacct gggcttcgcg
     1441 tatcccaacg atccagcagg agctgcactt gaatgaggct cagctcggcg cagttctttt
     1501 tgcgctgccg atcggcttgc tgggaacggc gcctgtcacc acggtcctgt taagccgttt
     1561 tgatagccgc cgtattatga taatcggagc aatcgctttc aatgtcatgc tttgtctcat
     1621 tggattcgca accaggacct gggagctggt gttggcgttg gtgtgcttcg ggtcttcgcg
     1681 caatctgatg aatatttcga tcaacgcgca gtccatcggc gtccaagcgc ttttcaaccg
     1741 ctccatcatc gcacgcttcc acggggtttg gagcctggcc gggtttgcgg gcgccgcagt
     1801 agggtcactc atggtgtctt tctcggttcc gccgctctgg catttcgctg cagtcgggtt
     1861 cctgttaagc atactttgtt ggctggcatt accggggacg gtgagacagc ggcctgctcc
     1921 gcgcgaacga agaccctggt tcactttgcc caataggagc ctggcaaaac tgggcctcat
     1981 atcattcgct tccatggcct gcgagggaac gatgtttgac tggagtggca tctatttccg
     2041 caaggcggtg cacgccggcc aggatgtggc aaccctgggt tttgttgctt atatgatcgc
     2101 catgacgctg ggccgcttga ccggcgaccg actggtaaac cgcttgggca tccgtactac
     2161 gctgacttac agcggcgggc tgatcggcct gggcttgcta accgccgttt gtctgccttt
     2221 tccgctgacg gcaggattcg ggttcattct taccgggttt ggcgtttcgt gcgtggtccc
     2281 catggtcttc ggcatggccg gtcgtacgac gggcatgagt agtggaagcg ccattgcttc
     2341 tgtttcaacg gtggcttata ccggattcct catcattccg ccactggtcg ggtccgttgc
     2401 acagcttgcc ggtcttcgga ccgcgtatac catgatcgcc tgtttcggtt tcctgatcac
     2461 cttcctggtg caattcaccc ttggcgaggt gcagccgctg ggtggcgcgg cgccgctcga
     2521 gtgagtcatt gattgcagcg atgatcacgg agtctgagcc ccgcagagcc gttgataccc
     2581 ggaaggttcg aaaaaagaga ccgcccactg cctgaaaggc agggacgatc cctacaaaga
     2641 caccagaaaa aaacagcgct attttgaacg atcgtcagaa cgtcggtccg tcacggacgc
     2701 cggtcaaatt accggcactg tcatccccac ccggatgctc ctatcgatct caactttaaa
     2761 tggtttgccg aatatgcggc tcgtcccgtt ccaggtactc tgaaccgtgt tgatagccac
     2821 aaatggtacg ggttttcgtt ggacctggaa gacaaacacg ggatcgcctt ctggtcgcgg
     2881 caagccccca tcgttcttat cgaccaggaa taaaggggaa agcaggtttt caaaccgcag
     2941 cgccatgttc aaaaaaatca gccgctttag ccggctgtct gcccggaacc gcagcacgcg
     3001 ctgcgggtat cgttccgtat agatctcgac gatttgaagc agggtacatg ttaccagcag
     3061 aaaatctttg ctttccggcc agttaggtgg ctccttgctt tccgccggtt tgttgcggaa
     3121 atcgaccagg taaatttgcc cgcccggctg gctgataaac ctaacctgcc tttcgattgg
     3181 gccgggcagg gtagggctaa tgaaattaaa attggcaaag tcgggcgctg cctgaagatt
     3241 agtataaggc gtgtattgca tataatatga tttgaaatag ctgcggccgg ttaaaacggc
     3301 cagccgcagc gtgacacgga tgatctattt tcccgatgct cctaccggct cttccgatac
     3361 ctcctggtcg ccagcgggtt tctccgctgc cggttccacg cttgcggccg atagccgcct
     3421 ggcggtggac cgcctttctt ccgatctcct cctttttggc ggacccggcg catagatcct
     3481 gatcgtcctc tccgcaggat gggcctcgat ccatttcggc tccggctttg tggtctccag
     3541 cgtgatcagg taccggttgc cgcgttccag tgccgaaagc agggcctttc tcgtgttagg
     3601 ggcgccgcat tccacgatcg ggtaattctc cagcaccttc tccaggtcga agtagcgata
     3661 gtacttgaac ttgtaattgc cgtgcatatc ccgttcttcg aaattcagcg tcacccagac
     3721 ggtgtacttt tcgtcatccc ggttgatcat gtccaccttg gccgcccttc catcaagcag
     3781 attgtaggcc tccttgaacg tgacaccaat tcccttatag atataaaagg accgcttttt
     3841 gtttcggtcg ggctcctgtt catactggat ctccgcctca taccgatcaa agacatagat
     3901 cccctgttga tcttgccggt gaaaatgcaa tttggcttct atcctgatga aatggtcaaa
     3961 aaaggcatcc gtcgtgagct caaactccgg ctcttcacgg gctatatggt cctcgagctg
     4021 ctggttcagc agcgtattgt ccccaaaccc caggtatttg aggcgtccat tgaggaattc
     4081 caggtttcca gtattcataa cataaaattt aataattgaa aaatagat
//
LOCUS       DATGKE010000646         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_995516, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000646 DATGKE010000000
VERSION     DATGKE010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995516"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2268
                     /gene="metG"
                     /locus_tag="VKU83_10910"
     CDS             124..2268
                     /gene="metG"
                     /locus_tag="VKU83_10910"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLI94115.1"
                     /translation="MMNQPKRYLITAALPYANGLKHIGHLAGAYLPADIYVRYLRARQ
                     RDVLFICGSDEHGTAIPIQAMKEGTTPRAIIDKYHERIKENFADLGISFDIYHRTSEP
                     IHHETAQEFFTYLNSRGELEVKETEQYYDEEAKTFLADRYIVGTCPVCANPRAYGDQC
                     ENCGSSLSPEMLIEPHSTLSGKPPVKRATRHWYLPLNKHEEFLREWILAGHKDDWRAN
                     VVGQCRSWIDGGLQPRAVTRDLDWGVKVPLAEAEGKVLYVWFDAPIGYVSATRQWSVD
                     SGKDWKPYWYDKDTKLVHFIGKDNIVFHCIIFPVMLKLHGNILPDNVPANEFMNLEGD
                     KMSTSRNWKLDMQDYIDDFMRKDNGGPQLADSLRYYLTTIAPETKDSEFTWKGFQNAN
                     NSELVSKFGNFVNRTFVLMHKLCGGKVPPLHAGALDVADREMIAALGSLPGKVSGLLE
                     EFRFREALFEVIMLSDLANKYMQKKEPWIVVRTLAANPENQQLIDNCLHICLQVTANL
                     AILINPFLPFTAKKMLHLMKVVDKMLDWENAGKMKLLSVGYSLRAPELLFRQIEDAEV
                     KAQLEKLKAASAARDAGPETAAGKAGQQDGQAGSGPAPVKEEIVYDDFARLDLRVGTI
                     LNAEKVEKADKLLKLTVDLGFEQRTIVSGIAMHFKPAEIIGRQVVVVANLAPRKMRGI
                     ESNGMILMAEDGAGKLYFVGPDQKVGAGSGVS"
     gene            2385..3023
                     /locus_tag="VKU83_10915"
     CDS             2385..3023
                     /locus_tag="VKU83_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94116.1"
                     /translation="MNQSHPYRILPYNRYSSFYQERESFYRSFVNQYYLNFAHQVLAQ
                     DEAEPFHTKTLIAPHDIHFGATPRRVIWKIGMPSFQAANINDLKDHKILFYRTKLLRQ
                     KALIQFHFINSFFYYSQLSFLSFTDTTNSILFNAIKGKYQLPSGHRGRDKIFMMDICG
                     NKLIIETGVYLTVSYVTGDSYPRLLMEEQIKQRDELLTRADNYEIDRLSKIL"
     gene            3149..>3408
                     /locus_tag="VKU83_10920"
     CDS             3149..>3408
                     /locus_tag="VKU83_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLI94117.1"
                     /translation="MNRIIKKVAVLGSGVMGSRIACHFAGIGVQTLLLDIAPKELTAA
                     EAAKNLSLAHPSVRNRIVHDALAAAIRSNPSPVYTSDVISRIT"
BASE COUNT          815 a          932 c          981 g          680 t
ORIGIN      
        1 tataaacgaa acgcatccgt cgaattcctg ctgcaggaat agccctcccc gcggcttccc
       61 gagtgggtca aaagccgtaa ttttgcgccc aacggacggg gcgtccgtcg acattagcca
      121 attatgatga atcaaccgaa acgatacctg atcacggctg cgcttcctta tgccaatgga
      181 ctgaaacata tcgggcatct cgccggggct tatcttccgg cggatatcta tgtccgctat
      241 ctgcgggcga ggcagcggga tgtcctgttc atctgcggca gcgacgaaca cggcacggct
      301 atcccgatcc aggcgatgaa ggaggggacg acaccccggg cgatcattga caagtaccat
      361 gaacggatca aagaaaactt tgccgacctg ggaatttcgt tcgatatcta tcaccggaca
      421 agcgaaccga tccatcatga gaccgcccag gagttcttta cctacctcaa cagcaggggc
      481 gaactggaag tgaaggaaac ggagcagtat tacgacgagg aagcgaaaac attccttgcc
      541 gaccggtaca tcgtaggcac ctgccccgta tgcgcgaacc cccgggctta tggagaccag
      601 tgcgagaact gcggatcgtc attgagtccc gaaatgctca tagagcccca tagcacgctc
      661 agcgggaagc cgccggtcaa acgggcgacg cggcattggt acttgccgct caacaagcac
      721 gaagaatttt tgcgcgaatg gatcctcgcc gggcacaagg acgactggag ggcgaatgtt
      781 gtcgggcaat gccgcagctg gatagacggg gggctgcagc caagagcggt gacgcgggac
      841 ctcgactggg gcgtgaaggt gccgctggct gaagcggagg ggaaggtcct gtatgtatgg
      901 tttgacgcgc cgatcgggta tgtctccgcc actcgccagt ggtccgtgga cagcggaaag
      961 gattggaagc cctactggta tgacaaagat accaagctgg tgcatttcat cgggaaggat
     1021 aatatcgtct ttcactgcat catcttcccg gtgatgctga agctgcacgg gaacatcctg
     1081 ccggacaatg tgcccgccaa cgagttcatg aacctcgagg gcgacaagat gagcaccagc
     1141 cggaactgga agctggacat gcaggattat atcgatgact ttatgcggaa ggacaacggc
     1201 ggcccgcagc ttgcggatag cctgcgctac tacctgacga cgattgctcc cgaaacgaag
     1261 gatagcgagt tcacctggaa ggggttccaa aatgccaaca acagtgagct ggtgagcaag
     1321 ttcggcaact ttgtcaaccg gacttttgtt ctgatgcaca aactctgtgg cggcaaagtg
     1381 ccgccgctgc atgccggtgc gctggatgtg gccgaccgcg aaatgatcgc ggcactggga
     1441 tccctgccgg gaaaagtatc cggcctgctg gaagaattcc ggttccgcga ggcgctcttc
     1501 gaggtgatca tgctgtccga tctcgccaac aagtacatgc aaaagaaaga gccctggatt
     1561 gtcgtgcgaa cgctggccgc gaacccggag aaccagcagt tgatcgacaa ctgcctgcat
     1621 atttgtctgc aggtaacggc gaacctggcg atcctgatca accctttcct gcctttcacg
     1681 gcaaaaaaaa tgctgcattt gatgaaggtc gtggacaaga tgctggattg ggaaaacgcc
     1741 ggaaaaatga agttgctgag cgtcggctac agcctgcggg cgccggagct gcttttcagg
     1801 caaatcgagg acgcggaggt gaaggcccag ctggaaaagc tgaaagccgc atccgcggcc
     1861 cgcgacgcag ggcctgagac agccgccggg aaggccggtc agcaggacgg ccaggcggga
     1921 tccgggccgg ccccggtaaa ggaagaaatc gtttatgacg attttgcccg gctcgacctg
     1981 cgggtcggga cgatcctcaa cgcggaaaaa gtggaaaagg ccgacaaatt gctgaagctg
     2041 acggtcgacc tgggtttcga gcagcgtacg atcgtctcgg ggatcgcgat gcattttaag
     2101 ccggcggaga tcattggccg ccaggtggtg gtcgtggcca acctggcgcc ccggaagatg
     2161 cgcggcatcg agagtaacgg gatgatcctg atggcggagg acggggcggg caagctgtat
     2221 tttgtcgggc cggaccagaa ggtcggagcg ggttccgggg ttagctgatt ggccttcaat
     2281 caaataacac ttattaatta tatcttggct gctacatact tcaagcagcc tgtagccgtt
     2341 aatattggac caaaagagcc taaggccatc taatatcacg tgctatgaac caaagccatc
     2401 cctaccggat cctgccttat aaccgctaca gttcgtttta ccaggagcgc gagagcttct
     2461 accggagctt tgtcaatcaa tactacctga attttgcgca ccaggtgctg gcgcaggatg
     2521 aggccgagcc gtttcatacc aaaacactta tcgcccctca cgacatccat ttcggggcca
     2581 ctccgcggcg ggtcatctgg aagatcggca tgccctcctt ccaggccgcc aacatcaatg
     2641 acctcaagga ccacaagatc ttgttctacc ggaccaagct gctccgccag aaggcgttga
     2701 tccagttcca cttcatcaac agcttttttt attactcgca actgagcttc ctttccttca
     2761 cggacacgac caacagtatc ctcttcaacg ccataaaggg gaaataccag ctcccttccg
     2821 gccaccgggg gagggataag atcttcatga tggatatttg cggcaacaag ctgatcatcg
     2881 agacgggcgt ctatctgacc gtgagttatg ttaccggtga cagctacccg cggctgctga
     2941 tggaagagca gatcaaacag cgcgatgaat tgctgaccag ggccgacaac tacgagatcg
     3001 accggctcag caagatcctc tgaccggccg gcttcccgcg tccaaatcca ccaggttatc
     3061 cgtcttcact gctgcccccg gtacgggctg taatgaattt ttgcctatct tagccgtaaa
     3121 tagttgttta actaactaat cgaagaccat gaaccgcatt atcaagaaag tggccgtatt
     3181 gggaagtggt gtaatgggct cgaggatcgc ctgtcatttt gcgggtatcg gcgtacagac
     3241 ccttttgctg gatatcgcgc caaaggaact gacggcggcc gaagcggcaa agaacctgag
     3301 cctcgcgcat ccgtcggtcc gcaaccggat cgtccatgac gcgctggcgg cggccatcag
     3361 gagcaatcct tcacccgtct ataccagcga cgtcatttcc aggatcac
//
LOCUS       DATGKE010000647         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_38936, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000647 DATGKE010000000
VERSION     DATGKE010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38936"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..792
                     /locus_tag="VKU83_10925"
     CDS             247..792
                     /locus_tag="VKU83_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HLI94118.1"
                     /translation="MTKHFALLVSLIAATNFLPTCTFGAPKDSLEVRKAVMGFQDDWN
                     HHDMAAFGRLFTMNAYFVNVAGAPMKGRDEIQLHHAWSHAAIPADSKVSGTSPGNYGI
                     FKNSTMHFTRIDINFLRKDVAVAHVSWELQGDARTPDPRHGVFMFVLTFQNKTWLIAT
                     AQNTEGNSKALQYLISTGLRK"
     gene            complement(1018..1284)
                     /locus_tag="VKU83_10930"
     CDS             complement(1018..1284)
                     /locus_tag="VKU83_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94119.1"
                     /translation="MRRLWLFYLIIFGPVAIWSIFIFHPALRSGISDTGFIIGLAFFL
                     FVYRPAICGLRLVATGKIKRSQFILNFIPFWSDKYWSFLFFNLN"
     gene            complement(1585..>2047)
                     /locus_tag="VKU83_10935"
     CDS             complement(1585..>2047)
                     /locus_tag="VKU83_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94120.1"
                     /translation="DSIRLLLPIDSNYMKFRPSVLINADKGDLDYGSGKWLGFHKNAL
                     SALLYYRKPVTVRDLTFSALVDIGAFIFPPVSLEVWGGPDEHHLKKLGALKPAQPEKA
                     GPPYLTGYEIAFPPQTVRCLKVLASPVKKLPDWHPGKGQPGWIFFDELLVN"
BASE COUNT          551 a          447 c          532 g          517 t
ORIGIN      
        1 tgagcccttc caccgctgtt cttccgatta taactaaaca gtggaacagt tttagaccag
       61 tgagatgatg agcattggtt ctccgatccg cgacggaaga agggcggttc cagggcagcg
      121 ggcatgcggg ggggtggggg ctgggaaggg gggggtaatt tgccttcaaa cgaaaatgaa
      181 aaaaaagtac ctttatttta ctttctctta ctatgaccta attgtaaata tctttaagca
      241 agagccatga ccaaacattt cgccttattg gtatctctga ttgcggcaac taattttttg
      301 ccaacttgca ctttcggtgc acctaaagat tctttagaag tcagaaaggc cgtaatgggc
      361 ttccaggatg actggaatca ccacgatatg gcagcatttg gaagactctt tacaatgaat
      421 gcttattttg tgaatgtagc aggcgctcca atgaaaggac gtgacgaaat tcaattgcat
      481 catgcatggt cacacgccgc tattcctgct gattcaaaag tttctgggac atcccctggc
      541 aactatggta ttttcaaaaa tagcaccatg catttcaccc gtattgacat aaactttcta
      601 agaaaagatg tagccgttgc tcacgtgtca tgggaactcc agggtgatgc ccgaacacct
      661 gatcctcgtc atggggtatt tatgtttgtt cttacttttc aaaataaaac gtggctgatt
      721 gctacagctc aaaacacaga agggaattcc aaagctttac aatacctgat ttcaacgggt
      781 ttaagaaagt gaaattattt gccctctccg tattttttaa gcgtaagtat caaaatcttg
      841 ccgagacccg gaagacaaat tattggtaac agcacgcatc agtgcctcgt tttgatattt
      901 ttgttttacc gctatgcttg cccgatcatt tttaaaaagc cttgaaagct aatactaaca
      961 tgtctttcaa aagcaacaag taccccggat ggggacttga ttaatattat gacgtgatta
     1021 gttgagattg aaaaacagga aactccagta tttatcgctc cagaatggaa taaaattcag
     1081 tataaattgg ctgcgcttga tcttccccgt ggcgaccagg cgaaggccgc aaatcgccgg
     1141 acgatataca aacaaaaaaa acgctaaacc tatgatgaaa cccgtgtcgc tgattccgct
     1201 gcgcagagcc ggatgaaata tgaatatgct ccagatcgcg actggaccga aaataatgag
     1261 atagaatagc catagccttc tcatagtctg aaattacgat ttattttttg atccgcaatg
     1321 gccaaccggc ggctgacgga gactgcgcgc atgggagcgg cctgtggtgc gctcatcttt
     1381 gaaaaggatt tcgacatttc gctgtccgcg ctgaaaaacg gccagaatca gttttcgacc
     1441 gaagtgaagc tggataaaga atgggcaaaa aagccttatc gtgtgctgat ctcggaatac
     1501 gagctgcatc cgttcgatcc ccgattggac gctcaagctg ttcaagttgc aacggggaca
     1561 gccgggatga acgtggagtt gaaattaatt caccagcagt tcatcaaaga atatccagcc
     1621 gggctggcct ttgccgggat gccagtcagg gagtttcttc acgggactcg cgagcacttt
     1681 gaggcagcgg acggtctgag gcggaaaggc gatctcataa ccggtcaggt aggggggccc
     1741 ggccttctcg ggttgggcgg gctttagtgc cccgagtttt ttgaggtgat gttcgtcggg
     1801 gccgccccag acttcaaggc tgacgggcgg gaaaatgaag gcgccgatgt cgacgagcgc
     1861 ggagaaggtg aggtcgcgga cggtgaccgg cttccggtag tagagtaaag cggaaagggc
     1921 attcttgtga aagccgagcc atttgccgct accatagtcg aggtcgcctt tgtcggcgtt
     1981 gatcaggacc gaggggcgga atttcatata gttggaatcg atcggcagca gcaggcgaat
     2041 cgagtcg
//
LOCUS       DATGKE010000648         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_452963, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000648 DATGKE010000000
VERSION     DATGKE010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452963"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VKU83_10940"
     CDS             complement(<1..406)
                     /locus_tag="VKU83_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94121.1"
                     /translation="MSQPLYPSAPAAVDLQNLAVSAGFRRQVPRVIWAILLFAAVYLL
                     LVAAAVGLAMGCGLLGVALIVRLTNFYGVIFGLGIIAVGVSVLIFLVKFVFAVSRDVN
                     SERIEVTEAEHPRLFAFVRSLAAETQTPFPKKI"
     gene            complement(411..1046)
                     /locus_tag="VKU83_10945"
     CDS             complement(411..1046)
                     /locus_tag="VKU83_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94122.1"
                     /translation="MRIVMGFLLVVAGLSCAAQDLDYPDFRSKKDNFTKIREQDIRND
                     VATFALAALDESIGKGQLQKVPVKDFGMNFMTFQNDNLQVTIRTGVFNPKKHKLMMSE
                     DHLLRIDGKPYYGGIYGQLPHTTIESITVVVDKDTVPIPAAAYIDLYDPKFSYNEGGE
                     SRTRNAVYLSNDKHTLYIYMFNNDNGGVEYTWVIRDKQYLRRVVDFGFLNK"
     gene            complement(1055..1900)
                     /locus_tag="VKU83_10950"
     CDS             complement(1055..1900)
                     /locus_tag="VKU83_10950"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HLI94123.1"
                     /translation="MKFLIGFLFPLLLSGAANAQYYYKDIISTRLNTAKWRAFKAAGV
                     RSVRLTSTEGDGRPTEGFEVEQTINADFSGMTTHSRTNGSIETWTFNTYSPQGLLTSV
                     TDTSDTYQSVSDYQYDTSGRLLSITNTSTETDNHVKAVETHIWEYPSATSAIPTGMLK
                     IQNGVDTTYVRFLPDEKGNLGEERARRNNEALPAIYYYYDDHSRLTDIVRYNLRAQRL
                     LPDNIFEYDEEGRLKSLIAVQVGASSYQKWIYAYNEKGLRTKETCYSKDGQLVGIVQY
                     EYNYK"
     gene            2024..3124
                     /locus_tag="VKU83_10955"
     CDS             2024..3124
                     /locus_tag="VKU83_10955"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HLI94124.1"
                     /translation="MSHLKERKEKNCLNCQATLYGRYCHVCGQENLEPKETVWHLVSH
                     FFNDITHFDGKFFSTVKYLLRKPGFLSKEYAAGRRASYLNPVRMYVFTSAIFFLILFA
                     LKNPRDMVTVNEKPDKQTAADLQQVPANLQRELASAGVDTTTRRIIGETARQWVPKRR
                     DSAGGNVYDNGPSFFGVNSGHYTSTAAYDSAQRALPDSLRDGWLKRALMNKIIAANIA
                     YNRDKKAYFEHVAENMLHSIPKVLFVSLPLFALILNIMYFRRRKRFYYVDHCIFTIHL
                     YCATFILTMAALLAYQLAQALHVGWLTTIIWLLIVAIWFYIIIYLYKAMRGFYGQGRF
                     KTFVKYCLVCLLSFTVNSIIFVIFALLSVVSV"
     gene            3134..>3463
                     /locus_tag="VKU83_10960"
     CDS             3134..>3463
                     /locus_tag="VKU83_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HLI94125.1"
                     /translation="MNETNSAFGRLVRIMDELREKCPWDRKQNIETLRPLTIEETYEL
                     ADAITAGDWSGIREELGDLLLHIVFYARIGREQGQFTLDEVIEGICDKLISRHPHIYG
                     TTAQEGLV"
BASE COUNT          790 a          877 c          968 g          828 t
ORIGIN      
        1 agatcttttt cgggaacggg gtttgggttt cggccgccag gctgcggaca aaggcgaaaa
       61 gccgcggatg ctccgcttcc gtgacttcaa tgcgctcgga attgacatca cgggagaccg
      121 caaacacgaa tttaacgaga aagatcagca cggaaacgcc gacggcaata atgccaagac
      181 cgaagatcac gccgtaaaaa ttcgtcagcc ggacgataag cgcgacaccg agcaaaccgc
      241 aacccatcgc gaggcccacc gctgccgcga cgagaagcag gtacaccgcc gcgaagagga
      301 ggatggccca tataaccctg ggcacctgcc ggcggaagcc ggcggagacg gcgaggtttt
      361 gaaggtccac tgccgccgga gcagaaggat ataatggctg actcatgcat tcatttgttc
      421 agaaaaccaa aatctacgac gcggcgcagg tactgcttat cccggatgac ccaggtgtac
      481 tcgacgccgc cattgtcgtt attgaacata taaatataca aagtgtgctt gtcattcgac
      541 aggtagaccg cgttccgggt acggctttcc ccgccttcat tatagctgaa tttcggatcg
      601 tagaggtcga tgtaggcggc ggccgggatc ggcaccgtgt ctttatcgac cacaacggta
      661 atggactcga tcgtggtatg gggtaattgg ccgtatatgc cgccatagta aggcttgcca
      721 tcaatccgaa gcaggtgatc ctcggacatc atcagcttgt gttttttcgg attgaagacg
      781 ccggtccgga tggtgacctg gagattgtca ttttgaaaag tcatgaaatt cattccgaaa
      841 tcctttaccg gcactttctg gagctggccc ttgccgatgc tttcatccag ggctgccagc
      901 gcgaaagtcg ccacgtcgtt gcggatatcc tgctcgcgga tcttggtgaa attgtctttt
      961 ttgcttcgga aatccgggta atcgagatcc tgggctgcac aggacaagcc ggctacgacc
     1021 aggaggaacc ccattacgat acgcatggca gtgatcattt atagttgtat tcgtactgga
     1081 cgatgcccac aagctgcccg tccttgctat aacaggtttc ttttgtcctc aggcccttct
     1141 cgttataggc atagatccat ttctgatagc tgctggcccc tacctgcacg gcgatgaggg
     1201 atttcagtct gccttcctcg tcgtattcga agatattgtc cggaagcagg cgctgcgccc
     1261 ggaggttata cctgacgata tcggtcaacc ggctgtggtc atcgtagtaa taatagatag
     1321 cgggcaacgc ttcattgttc ctcctcgcgc gctcttcgcc caggttccct ttctcgtcag
     1381 ggaggaagcg gacataggta gtgtctacgc cgttttggat cttcagcatg ccggtgggaa
     1441 tggcgctggt cgccgacgga tattcccaga tatgggtctc gacggccttg acatggttgt
     1501 ctgtttcggt cgaggtattg gtgatggaca acaacctgcc gctcgtatca tactgatagt
     1561 cggagacgct ctgataggta tcgctggtgt cggtgacgct ggtaagaagg ccctggggcg
     1621 aataggtatt gaaggtccag gtttcaatgc tgccgttcgt tctggagtgc gttgtcatgc
     1681 cggaaaaatc ggcgttgatc gtctgctcga cctcgaaccc ttcggtcggc cggccatccc
     1741 cttcggtgct cgttagccgt accgatctca cgccggctgc cttaaatgcc cgccatttgg
     1801 cggtattcag ccgggtggaa atgatgtctt tgtaataata ctgagcgttg gcggccccgg
     1861 atagcaagag cggaaaaaga aatccgatca aaaatttcat tggacaaatt taggaattcc
     1921 attataccgg cccttggccg gtatgagcga tagaacgaaa aaaacgccgt tttaatacgt
     1981 ctggcgtggg ccaaatgact attttagtaa aaatttaaac cgtgtgtcgc acctcaagga
     2041 aaggaaggaa aagaactgcc tgaactgcca ggcgactcta tatggccgtt attgccatgt
     2101 ctgcgggcag gagaatctcg aaccgaaaga aacggtgtgg cacctggttt cccatttctt
     2161 caacgatatc acgcatttcg acggcaagtt cttttccact gtgaaatacc tgctgaggaa
     2221 accgggtttc ttgtcaaagg aatacgctgc aggccgccgg gcaagttacc tgaaccccgt
     2281 ccggatgtat gtttttacgt cggcgatatt ctttctgatc ctttttgccc taaagaaccc
     2341 cagggacatg gtgactgtca acgagaagcc ggataagcaa acggccgcgg acctgcaaca
     2401 ggtaccggca aacctgcagc gggagctggc gtcggccggc gttgacacga ccacccggcg
     2461 aattataggt gaaactgccc ggcagtgggt gccgaaacgc agggacagcg ccgggggaaa
     2521 tgtgtatgac aatggtccaa gcttcttcgg ggtgaattct ggccactaca cgtccacggc
     2581 ggcctacgat tctgcgcaaa gggcattgcc ggacagcctg cgcgacggct ggctgaagcg
     2641 ggccctcatg aataagatca tcgccgccaa tatagcgtac aacagggata agaaggcgta
     2701 ttttgagcac gtggccgaga acatgctgca ttccattccg aaggtattat tcgtatcgct
     2761 gccgcttttc gcgctgatct tgaatatcat gtatttccgg cgccggaaaa ggttctatta
     2821 cgtggaccat tgcattttca cgatccatct ctactgcgcg acctttatcc ttacgatggc
     2881 cgccctgttg gcgtaccagt tggcgcaggc gttgcacgtc ggttggctga cgaccatcat
     2941 ctggctgctg atcgttgcga tctggttcta tatcatcatc tatctataca aggcgatgcg
     3001 cggcttttac ggccagggaa ggttcaagac ctttgtcaag tattgcctgg tctgcctgtt
     3061 gtcctttacc gtgaactcga ttatctttgt gatcttcgct ttgttgtccg ttgtttcggt
     3121 ataaatttcc attatgaatg aaacgaatag tgcttttggg cggctggtaa ggattatgga
     3181 cgagttgagg gagaaatgtc cctgggaccg gaagcagaac atcgaaacgc tgcgtcccct
     3241 cacgatcgaa gagacatatg agctggccga cgcgatcacg gcgggagact ggagcggcat
     3301 ccgggaggaa ctgggcgacc tgcttctgca tattgtgttt tatgcccgga tcggaaggga
     3361 acaggggcag ttcacgctcg atgaggtcat tgaaggaatc tgcgacaagc tcatttcaag
     3421 gcacccccac atctatggaa cgacggccca ggaaggactg gta
//
LOCUS       DATGKE010000649         5738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_972277, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000649 DATGKE010000000
VERSION     DATGKE010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972277"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VKU83_10965"
     CDS             complement(<1..355)
                     /locus_tag="VKU83_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94126.1"
                     /translation="MGPRYPFRILRLAFRNLHFLMFLNGFLLASLFYFRMESFYEDGL
                     FASIKSTIDARIDSNDNTDSLVVKAMDICYHLLSNKGRTFANAELGPEADVIHSTSFD
                     LMTTRGACGSYSQVLA"
     gene            complement(528..2630)
                     /locus_tag="VKU83_10970"
     CDS             complement(528..2630)
                     /locus_tag="VKU83_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94127.1"
                     /translation="MFPLPTRGVRPRCLLMTLGLVLAIAADSQTTSQPSSRPLASPGD
                     SGENWVIRADSIDPAHYFGVTVANGMIGLVSSADPFRAKDIVLNGAFDLYGRGRVSNI
                     LKTFNFINLNLDIDGNRISSFRQVSGLSQVLDMKNACLTTKFDYGDKATVSYTWYSLR
                     QLPYTALVDVSITAHKDIEIVPASVMEAPDMLRDVENYFNQIDRPHVLVSLLTSTARS
                     PTGKLLMAASTSFIFDEPHGAQPAVIHELWDNNMHLMKFRKKLTAGTTYHFAVVGSSL
                     TTAHNDDPLNEAERLTLFALLQGSRQLIKDHEAAWADLWKSDIIIDGDNRTQRDVRLM
                     LYHLYSFARQGTDYSLSPMGLSGLGYNGHTFWDTEIWMYPALLLLHPEIAASLMEYRY
                     RRLPAAMRNAAEHGYKGAMFPWESAASGNEETPVWALSGPFEHHITADVGIAAWNYYC
                     VTRDKGWLREKGYPILQATADFWASRVQRNGPGHYDINNVVAADEWAENVDDNAYTNA
                     AARANLQDAIEAARVLGVSPDPDWSAVRDNIPILRFPDGTTREHRTYNGELIKQADVN
                     LLAYPLKEVTSPAAIRRDLEYYEPRVGEGPAMTHAIFAILYERLGMPDKAYRAFEKGY
                     LPNLRPPFGVMAETATGDNPYFATGAGGMIQAMLNGFGGLDITPAGIVQLPTRLPNNW
                     KSITITGIGPGHTTYSVR"
     gene            complement(2682..>5738)
                     /locus_tag="VKU83_10975"
     CDS             complement(2682..>5738)
                     /locus_tag="VKU83_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLI94128.1"
                     /translation="FKDATAEARIPQDGGWSTGVSVVDINNDGLLDIYICRVGNHETL
                     HSHNQLLVCQGIDKNGVPWYKDEAKEYGLDFSGFSTQAVFFDYDMDGDLDMFLLNHQP
                     HQNGAYGPRAEMLRTFSPLSGDRLYRNDGKGHFTDVTKESGINSSAIGYGLGIVASDI
                     NLDGYPDLYVDNDFHENDYLYINQKNGKFREELNDHIMHTSKFSMGVDVADATNDGFP
                     EIMTVDMLPYEPSMLKSSEGENTWDAFYLKIGLGYNFQYGRNNLQYNRRNGMFSEAAL
                     YGGIAATDWSWSPLFFDFDNDGLKDLFISNGIPRRLNDIDYINFVSNIELQEKLQAES
                     SGHEELDLIEKSPQIKIPSKFFKNNGNLQFLDIGDEIGNAKRAYSNGAIYADLDNDGD
                     LDVVVNNIDEPALLYDNLSNDKKTRPFVEVRLKGPAQNVNALGAKVILFAGGGIRTYE
                     KYPVRGFLSSSEIPIHIGLDRTTIDSAFLVWPDNSFQPIQLSPRTPWVTFQYRKGLPK
                     FDYSRLAAHSPNPTKPMEDITARTHLLYRHEEDNYNEFDREPLIPHMLSAEGPALAVG
                     DLNGDGLDDVFIGAAKNKRSGLFFQDRSGKFVRSNQPVLDGDSAFEDVDACIADVNHD
                     GKPDLVVASGGGEVYGTDPHLAPRVYLNDGRGHFTKDSTAFDSLYVNASTVLACDVNG
                     DGLPDLFIGGRSVPYDYGKIPRSCLLLNDGRGHFTDVTDKYAPGLSHIGMVTRGIWFD
                     LDQDGRKDLILSLEWGGIVAFMNRNGSFVKKELTGRRGWWNFILPVDLNHDGHTDLIA
                     GNQGLNSKLKATEQEPVRMYYYDFDGNGKKEQVLTYYLDGQEIPFPTMAELEQQMPSL
                     KKKYLYAGDFAKASLPDIFTSGMLEKADTFTADYFPNAVLMAQGNGAYAAKALPWEAQ
                     LSPYRDAIVVNANDDSLPDILLVGNYYENTIQMGRNDADFGTILVNRGNGSFSAEPIN
                     GLAIKGEVRHISRIRIGGKEAFVLARNNDSAMVIRFTPRPVPSRAP"
BASE COUNT         1182 a         1517 c         1705 g         1334 t
ORIGIN      
        1 gggcgaggac ctgcgaataa cttccgcagg cgccgcgggt ggtcatcagg tcaaaagaag
       61 tggagtggat gacatcggct tcgggtccaa gctccgcatt ggcaaaggtt ctccctttgt
      121 tgcttagtag gtgatagcag atatccattg cttttaccac cagtgaatcc gtattatcgt
      181 tggaatcgat ccgggcgtcg atcgtagatt ttatcgaggc gaagaggcca tcttcgtaga
      241 aggactccat tctaaaataa aacagggagg ctaacaaaaa cccattcagg aacattagga
      301 agtgcaggtt ccggaacgca agccttaaaa tacggaaggg gtaccttggt cccatagttt
      361 tttcaagtgg ttaagagtta gctgatagga tttggattaa ggggcccgag aaaaatacgg
      421 ttggcttagt aaataacgca tatggcgtca tttattgtct gcgacggatt gaaacgcgaa
      481 gtaataccgg gcgggccggg gtgggcggac ccagaccccg gtcaatccta cctcactgaa
      541 taagtggtat ggccagggcc aattccggta atagttattg atttccaatt gtttgggagg
      601 cgcgtaggca attgcacaat gcccgcaggc gtgatatcga ggccgccgaa gccattcaac
      661 atcgcttgga tcattccgcc cgcgccggtc gcaaaatagg ggttgtcgcc ggttgccgtt
      721 tcggccatga cgccaaacgg gggccgaagg ttgggcaggt accccttttc aaaggcgcgg
      781 taagccttgt ccggcatccc gaggcgttca tagaggatcg caaaaatcgc atgcgtcatg
      841 gcagggcctt cgccgacccg cggttcatag tattccaggt cccgccggat tgccgcggga
      901 gaagtaacct ctttcagcgg ataagccagg aggttgacgt ctgcctgttt gatcagttcc
      961 ccgttatagg tccggtgctc ccgggtcgtc ccatcaggaa accgcaagat gggaatattg
     1021 tccctgaccg ccgaccaatc cggatcggga ctgacaccca ggacccgtgc tgcctcgatg
     1081 gcatcctgca gattcgcccg ggccgccgca ttcgtatagg cattgtcgtc gacgttttcc
     1141 gcccattcat ccgccgccac gacgttattg atgtcataat ggccggggcc gttgcgttga
     1201 acgcggctgg cccagaagtc tgcggtggcc tggaggatcg ggtatccttt ttccctgagc
     1261 cagcccttgt cgcgggtcac gcaatagtaa ttccatgccg cgatgcctac gtcggcggta
     1321 atatggtgtt cgaatgggcc gctcagcgcc catacgggcg tctcttcatt gccgctggcg
     1381 gcgctttccc atgggaacat tgcgccttta tagccgtgct cggcggcatt ccgcatggcg
     1441 gccggcagcc ggcggtaccg gtattccatc agggaggcag cgatctccgg atgcaggagg
     1501 agcagcgcgg ggtacatcca gatctccgtg tcccagaagg tatggccgtt atagcccaac
     1561 ccgctgaggc ccatcgggga aaggctgtaa tcggttccct ggcgcgcgaa ggaatacagg
     1621 tggtatagca tgaggcgcac gtcccgctgc gtccgattgt cgccatcgat gatgatatcg
     1681 ctcttccaaa ggtccgccca tgccgcttca tggtccttga tgagctggcg gctgccctgc
     1741 agcagtgcga agagggtgag gcgttccgct tcgttgagcg ggtcatcgtt gtgagcggtg
     1801 gtaagggaag agccgacaac ggcaaagtgg taggtcgtgc ctgccgtcag ctttttcctg
     1861 aatttcatca ggtgcatatt gttatcccag agctcgtgga tgacggcagg ctgggcgccg
     1921 tgcggctcgt cgaatatgaa gctggtggat gctgccatca ggagcttgcc ggtgggactt
     1981 ctggcggtcg aagtgagcag tgaaaccagg acgtggggac ggtcgatctg gttaaaataa
     2041 ttctccacgt cccggagcat atccggggcc tccataacgc tcgccgggac gatctcaatg
     2101 tccttgtgcg ccgtaatact cacgtctacc agtgccgtat agggtaattg ccgcagggag
     2161 taccaggtat agctcacagt ggctttgtcc ccgtaatcga acttcgtggt caggcaggcg
     2221 ttcttcatat ccaacacctg gctgaggccg gacacctggc gaaaagaact gatccggttg
     2281 ccgtcgatat cgagattcag gttgataaaa ttgaaagtct tgaggatgtt gctgacacgg
     2341 ccgcggccgt acaggtcgaa tgcgccgttg agtacgatgt ccttcgcgcg gaaaggatcc
     2401 gccgaagaga cgagaccgat cattccgttg gccacggtga cgccgaagta atgagcgggg
     2461 tcaatgctgt cggcccggat aacccaattt tcgccggagt cgcccgggga tgctaaaggc
     2521 cggctggaag gctgtgaggt cgtttgactg tcggcggcga tggcgagcac gagtcccagc
     2581 gtcatcagca ggcaacgggg acggaccccg cgagtaggta atggaaacat gcgatgaatt
     2641 tacggaattc gggttacaga ccgagctgta aaacgatgaa tttatggagc ccgggatgga
     2701 accggtcggg gcgtgaaacg aataaccatc gcgctatcgt tgttgcgggc cagaacaaat
     2761 gcttccttgc cgcctatccg gatgcggctg atatgccgca cctcgccttt gatggcaagc
     2821 ccgttgatcg gctccgcgga gaacgagccg ttcccgcggt ttacgagaat ggtgccgaag
     2881 tcggcgtcat ttcttcccat ttggatcgtg ttctcataat agttccccac cagcaggata
     2941 tccggcaggc tgtcgtcatt ggcattgacg acgatggcat cccggtaggg cgataactgc
     3001 gcttcccacg gaagggcctt tgccgcatag gcgccattcc cctgagccat gagcacggca
     3061 ttcgggaagt agtcagcggt aaatgtgtcc gctttttcca gcatcccgga cgtgaagata
     3121 tctggaaggg aggctttggc gaagtcgccg gcatacaaat actttttctt gaggctcggc
     3181 atctgttgtt ccagttcagc catggtcgga aacgggatct cctggccgtc caggtagtag
     3241 gtgagcacct gttctttttt gccattcccg tcaaagtcat agtaatacat gcgcaccggc
     3301 tcctgctctg tggctttcag cttgctattc agtccctggt tgcccgcgat aaggtcggta
     3361 tggccgtcat ggttcagatc gactggcagg atgaagttcc accagccccg ccggcctgtc
     3421 aattcctttt tgacgaagga accgttgcgg ttcatgaaag cgacgatgcc gccccattcc
     3481 agggagagga tcaggtcttt cctgccgtcc tgatcgagat cgaaccaaat gcccctcgtt
     3541 accattccga tatgggagag gcccggcgca tatttgtccg tgacgtcggt gaaatggccc
     3601 ctgccatcgt tcagcagcag gcaagaccgc ggaattttgc cataatcgta gggaacagac
     3661 cggccgccga taaaaaggtc cggtaagcca tcgccgttga catcacaggc cagaacggtc
     3721 gacgcattga cgtagaggct gtcgaaagcg gtggagtcct tcgtgaaatg gcccctgccg
     3781 tcgttcaggt atacccgcgg cgcgaggtgc ggatcagtcc catagacctc gccgccgcca
     3841 ctggctacga cgaggtccgg cttgccgtca tgatttacgt ccgcaatgca ggcgtcgacg
     3901 tcttcgaatg cgctgtcgcc gtcgagaacc ggctgattgc tgcggacgaa tttgccggac
     3961 cggtcctgga agaacagacc actcctctta ttttttgcgg ccccgatgaa aacgtcatcg
     4021 agtccgtcgc cgtttaggtc gccgacggcc aacgcaggcc cttctgcgga gagcatgtgc
     4081 gggatcagcg gctcgcgatc gaattcatta tagttgtctt cctcgtgacg gtacaacagg
     4141 tgggttcggg ccgtgatatc ttccattggt tttgtcgggt ttggcgagtg cgcagccagg
     4201 cgggaataat caaactttgg caggcctttc cggtattgga aggtcaccca gggggtgcgt
     4261 ggactcagct ggattggctg aaagctattg tccggccaca cgaggaaggc ggagtcgatc
     4321 gtcgtccggt cgagcccgat atggatcggg atctctgaac ttgataaaaa gccgcgcacc
     4381 gggtattttt cataggtccg gatgccaccg cccgcaaaaa ggatgacctt cgccccgagc
     4441 gcattgacat tttgtgccgg gcctttcaac cggacttcga caaatggccg ggttttcttg
     4501 tcgttggata aattatcata gagcagtgcg ggttcatcga tattgttcac gacgacgtcc
     4561 aggtccccgt cgttatcgag gtcggcatag atggcgccat tggaataggc gcgcttcgcg
     4621 ttgccgatct cgtcgccgat gtccaggaat tgcaggttgc cgttattctt gaagaatttg
     4681 ctggggatct ttatttgtgg ggatttttcg atgaggtcga gctcttcgtg ccccgagctc
     4741 tcggcctgca atttctcctg cagctcgata ttcgaaacga aattgatgta gtcgatatcg
     4801 ttcagccttc tggggattcc gttggagatg aagagatctt tcaggccgtc gttatcgaaa
     4861 tcgaaaaata gcggcgacca ggaccagtct gtggccgcga tgccgccgta gagcgcggcc
     4921 tcgctgaaca tgccgttgcg ccggttgtat tgcaggttat tccggccgta ttggaagttg
     4981 tagcccaggc cgattttcag gtaaaacgcg tcccaggtgt tctcgccttc agaacttttc
     5041 agcatacttg gctcgtaagg cagcatgtcg accgtcatga tctcggggaa accgtcgttg
     5101 gtggcatccg ccacatcgac gcccatcgag aatttgctgg tatgcatgat gtggtcattg
     5161 agctcctccc tgaatttacc gttcttctga ttgatgtaga gatagtcgtt ttcatggaaa
     5221 tcattatcga catagaggtc cggatagccg tcgagattga tatcggaggc gacgatgccg
     5281 agtccgtacc cgatggcgga gctattgatg ccggactcct ttgtcacgtc ggtgaagtgt
     5341 cctttgccgt cgttacggta aaggcgatcg ccggagagcg ggctgaaagt gcgcagcatc
     5401 tccgcccgtg gcccataggc gccgttttga tgcggttggt gattcagcag gaacatgtcc
     5461 aggtcaccgt ccatatcata atcaaaaaag acggcctgtg tgctgaagcc ggagaaatcc
     5521 aacccgtatt cctttgcctc gtccttgtac cagggcacgc cgttcttgtc tatcccctga
     5581 cagaccagca actggttgtg ggaatgcaag gtttcatggt ttcccacccg gcagatatag
     5641 atatcgagca agccgtcatt gttgatatct acaaccgaca cgcccgtgga ccagccgccg
     5701 tcctggggta tcctcgcctc tgcggtggca tccttgaa
//
LOCUS       DATGKE010000650         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_657468, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000650 DATGKE010000000
VERSION     DATGKE010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657468"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="VKU83_10980"
     CDS             <1..2080
                     /locus_tag="VKU83_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI94129.1"
                     /translation="MFTYPLLAGDRKTALKDPFTVVLTESAARRVFDVKRDNLASLLG
                     KAILIGNDSMPFKITGVCEDVPENSHLQFDLLVSYVTLYSGKNPYKEADYDFRDSDFW
                     HYIQLRHGADYKALEAKLPAFSQKYFQGNKVSGSVEKFYLQPLARAHLYSDFEYEIGK
                     TGSATVVWGLLIIALFIIVIAWVNYVNLATARSVERAKEVGVRKVIGAEKGQLIRQFL
                     TESFLINVFALILGIALMISLQHPFNSLVGHKLSYHYLMQKSLGGYAISFGLLALVLA
                     GIFVSGFYPAFVLSSFRPILVLKGRFSSSKAGIILRKGLVTGQFAITVVLIIGSFVVY
                     RQIRFMTNQELGMNIDRMLIVNPPRLTNWDSTFISREDAFTHDLGLIPGVAGAATSWN
                     TMGGETGRSFNVRRADQPGTVHYTMRHTGISNGFLKLYDIKVIAGRDFTNTDFNPDFG
                     KLHNIILNANATKLLGFKSPADAIGKSIMRGEKKWDVVGVINDYHQKSLRYPIEPTML
                     MPAYGTGSSISVKVNTANMPATIGAIKKSFDAFFPGNYFEYYFLDQHYNEQYANDQLF
                     GKAFGIFAGFAIFIACLGLLGLSLFATMQRTREIGVRKVLGASVPSIVGLLSRDFMLL
                     VGFSFLIASPVAWWIMHRWLQDFAYRIHMEWWVFAGAGVLAAGIAMATISFQAVKAAM
                     ANPVKSLRTE"
     gene            2392..>3063
                     /locus_tag="VKU83_10985"
     CDS             2392..>3063
                     /locus_tag="VKU83_10985"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLI94130.1"
                     /translation="MRSPLVLLFLFSVCLDCCSQGLTSPQIDSLAERARRAFDVPGIA
                     VCVIKDGKVIHSKGYGVRSLNTGEPVDENTLFGIASNSKAFTTAALGMLVDEGKLSWD
                     DKVRKYIPEFKLSDPYVTEEFTIRDLLCHRSGLGLGAGDLMFFPDGSNFTIPDILHNL
                     QYLKAASSFRSQYAYDNNLYIVAGEVVARASGMSWEDFIDTRILRPLGMTHSAASFVR
                     LRDSSD"
BASE COUNT          664 a          805 c          843 g          751 t
ORIGIN      
        1 gatgttcacc tatccgctgc ttgcgggcga ccgcaagaca gccctgaagg atccctttac
       61 agttgtcctg accgaatccg ccgccaggcg ggtcttcgac gtgaagcgcg acaatctcgc
      121 ttcccttctg ggcaaggcca tcctgatagg caatgactcg atgcccttca agataacggg
      181 cgtctgcgag gatgtcccgg agaactcgca cctccagttc gatctgctgg tttcctatgt
      241 gaccctgtat tccggaaaaa atccctataa ggaagccgac tatgatttca gggattccga
      301 tttctggcac tatatccaac tgcgtcatgg cgctgattac aaggcgctgg aggcgaagtt
      361 gcccgccttt agccaaaaat attttcaagg caacaaggtt tccgggagcg tggagaaatt
      421 ctacctccag ccgctggcca gggctcacct gtactcggac tttgaatacg agatcggcaa
      481 gacgggcagt gcgacggttg tctggggcct gctgatcatc gccttattca tcattgttat
      541 cgcatgggtc aattatgtca atctcgctac cgcgcgatct gtcgagcggg ccaaggaggt
      601 cggcgtaagg aaggttatcg gcgctgaaaa ggggcaactg atccggcaat tcctcaccga
      661 atcctttctc attaatgtct tcgcgttgat tctgggaatc gccttgatga tcagtttgca
      721 gcatcctttc aattcactgg tcggccataa gctaagctac cattacctga tgcaaaaaag
      781 tctcggcggt tacgcgatca gctttggatt gttagcgctc gtactggccg gcatctttgt
      841 ttccggcttc tatccggctt ttgtgctgtc ctctttccgg cccatcctcg ttttgaaggg
      901 tcgcttcagc tcgtcgaagg ccgggatcat tttgagaaag ggcctggtta ccgggcaatt
      961 tgcgatcacc gtcgtcttga tcatcggctc ctttgtcgtt tacaggcaga tccggttcat
     1021 gaccaaccag gagctgggca tgaatatcga ccggatgctg atcgtcaatc caccccggct
     1081 gaccaactgg gactccactt ttatcagcag ggaagacgcc tttacccatg acctcggcct
     1141 aatccccggg gttgccgggg ccgccacctc ctggaatacg atgggcggag aaacagggag
     1201 aagctttaat gtccggcggg ccgaccaacc gggcaccgtt cactacacga tgcgacatac
     1261 cggcatcagt aatggcttcc tgaagttgta tgatatcaaa gtgatagccg gccgggactt
     1321 taccaatacc gatttcaacc ccgacttcgg aaagctgcac aatattatcc tgaacgccaa
     1381 cgccaccaag ctgcttggct ttaaatcccc ggcggatgcc atcgggaagt ccatcatgcg
     1441 cggggaaaag aaatgggatg tcgtcggcgt tattaacgac tatcaccaga agtcgctccg
     1501 ctaccccatc gagccgacga tgttgatgcc tgcttatggc accggaagct ccatctcggt
     1561 aaaggtgaat acggcgaata tgcccgccac gatcggggcc ataaaaaaat cgttcgacgc
     1621 cttctttccc ggcaattact tcgagtatta tttcctggac cagcattata acgagcagta
     1681 tgcgaatgac cagctgttcg gtaaggcatt cggcatcttc gcggggtttg cgatcttcat
     1741 tgcctgcctc gggctgctgg gtctctcgct gttcgcaacg atgcagcgga ccagggagat
     1801 cggcgtccgg aaggtactgg gggcgtcggt gccgagcatc gtcggcctgt tgtcgcggga
     1861 ctttatgctg ttggtcggct tctcgttcct gatcgcgtcg cctgtggcct ggtggatcat
     1921 gcaccggtgg ctgcaggatt tcgcttaccg gattcatatg gagtggtggg tgttcgccgg
     1981 cgcgggagtg ctggccgcag ggattgccat ggcgacgatc agtttccagg cggtaaaggc
     2041 ggcgatggcc aatccggtga aaagcctgcg aacggagtag gcggcacgcc ggcaagcgaa
     2101 aaaaataagc ccctgtagaa acaggggccg taaccaaaac taactgctta tgagaaaatt
     2161 gactgcttga gaaactttag gcttaccttt cggatccgcc ctttataatt tttcgatttt
     2221 actttcgatt tcttgttccg ctttctgatg tcaaagttag gatgcgcaat gttaagccca
     2281 ctgttaaaaa atgcttatag cggggagagt gccgcgattt aatgtttttt taatgttttt
     2341 gatccggctg gggaggtttc cttatattta gttgactaaa agctaattgt catgagatcc
     2401 ccgctggtcc tcctgttcct tttttccgtt tgcctcgact gctgttcgca gggcctcacg
     2461 tctccacaga tcgacagtct ggccgagcgg gcccgaaggg catttgatgt gcctggaatt
     2521 gccgtttgcg tgatcaagga cggaaaggtc attcattcaa agggttatgg cgtgcgctcg
     2581 ctgaacacgg gcgagccggt agacgaaaac accttgttcg gaattgcctc aaacagcaag
     2641 gcttttacca cggccgcgct gggaatgctg gtagacgagg gaaaactatc ctgggatgac
     2701 aaggtccgca aatacattcc ggaattcaag ctttccgacc cttatgttac ggaagaattc
     2761 acgatccgcg atttactgtg tcaccgcagc ggactgggcc tgggagcggg tgacctgatg
     2821 ttctttcccg acgggagcaa ttttacgatc ccggatattc tgcacaacct gcaatacctg
     2881 aaagcggcaa gcagcttccg gtcgcaatac gcctacgaca acaacctgta tatcgtcgca
     2941 ggggaggtgg tcgcccgggc aagcgggatg agctgggaag attttatcga tacgaggata
     3001 ctcaggccgc tgggaatgac gcatagtgcc gccagcttcg tccggctccg ggacagctcg
     3061 gat
//
LOCUS       DATGKE010000651         6242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_765178, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000651 DATGKE010000000
VERSION     DATGKE010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765178"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VKU83_10990"
     CDS             complement(<1..1645)
                     /locus_tag="VKU83_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLI94131.1"
                     /translation="MNTYDTIVIGSGISGGWAAKEFCDHGLNTLMLERGRPVQHLKDY
                     PTATTPPWGFAHRGAMPLATLEENPVVSKCYAYGEATQHFFVPDKFHPYIQEKPFDWI
                     RGYQVGGKSLLWARQTQRWSPFEFEGPARDGIAIPWPIGYNDVAPWYSYVERFVGISG
                     NKDGLPNLPDGEFLPPWELNCVEEHIRQGIHSRYPGRPVIIGRCAHLTKPQPIHIEQG
                     RGQCQARSMCDRGCPFGGYFSSNSSTIPWAQKTGKLTLQPFSVVHSIIYDESKGKAVG
                     VRVIDAQTRKATEYFARVIFVNAACLNTNLILLNSVSRRFPNGLGNDHGILGHYVAFH
                     NYRGSLNAEFEGYPDRYYYGRRPTEVFIPAFRNVFRDEGLGFKRGYMAYYGASRAGWG
                     RGDYRDGVGGDYKDSLSEPGSWFVSMGMQGETIPKFENHVRLSSDQKDQWGIPQLITS
                     VGYDDNDAKMCADFLARGAEMLEAGGCKNISGEDNHQNPGLDIHEVGGCRMGKDPKTS
                     MLNEWNQLHACKNVFVTDGACMTSTGNQNPSITFMALTAR"
     gene            1677..3212
                     /locus_tag="VKU83_10995"
     CDS             1677..3212
                     /locus_tag="VKU83_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HLI94132.1"
                     /translation="MRKFIALLIIVADALGSFGQGFETIKVEGGLISGAMDTRGDIHS
                     FKGIPFAAPPVGPLRWKAPQPVVPWTGVRSCDQFGASPMQNKPAAFLMWTEEYLIAPT
                     PISEDCLYLNVWSGARTPGERRPVLVWIYGGGFSSGGGNVPIYDGTAMARKGIIFVSI
                     NYRVGIFGFFSDPELSSESGHQASGNYGLMDQIAALKWVRNNIAAFGGDPNNVTIAGQ
                     SAGSMSVNCLVASPLAKGLFQKAIGESGGFFSRDMLSLGEAEESGRKLMQSLGITGLE
                     AMRAIPADELLKKGGALHTFPVVDNYVLPAQVADIFAAGKENPVSLLTGWNEDEGFVF
                     GPTKKAADYRQDIEKEYGAAAATVLKYYPADNDSMAGVSQVRLSRDMIFGRQNYAWAN
                     VASSHGRRVFAYRFVRRPPTAPGGRRWGAFHTAEVPYAYDNLQFVHRPWEPLDYNLAD
                     EMSTYWANFVKTGDPNGIGLPRWPLYTAKDPQIMVFGRQAAAGVLADKAALDFQLTRM
                     EGR"
     gene            3324..3983
                     /locus_tag="VKU83_11000"
     CDS             3324..3983
                     /locus_tag="VKU83_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94133.1"
                     /translation="MPWTRSTILIIDHDDTAAAELAGLLKNRPQPPAVFVAPGVGTAE
                     PVLRTESIDWMFIRIIAWDDFQRLRPLLLSAPRRVVFLSGRNEKCTAHLPLVLDSHLQ
                     PPYRSSHLVRAWNKWMDPLFIPRPLDIFFIKSRARFEAVRYGDLRQVEVEDGKLRLST
                     RHADYQVTGSLQAFRDRLPISLALVRRGCLVNEFYLPAETNSLYRVSSGTSVGLSNSR
                     G"
     gene            complement(3905..4342)
                     /locus_tag="VKU83_11005"
     CDS             complement(3905..4342)
                     /locus_tag="VKU83_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI94134.1"
                     /translation="MNPPNLKLAVPFFGVTDMEKSLDFYVRGLGFTLKHQWTPRGKIE
                     WCWLTRDGVSLMLQEPRSETDPLRNSSQTKAFGVNICVQCEDALALYREFKARHLPVT
                     EPFVGNNMWVFKVRDPDGYPLEFESPTEVPEETRYSEFVSAGK"
     gene            complement(4385..5890)
                     /locus_tag="VKU83_11010"
     CDS             complement(4385..5890)
                     /locus_tag="VKU83_11010"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HLI94135.1"
                     /translation="MADRPTYWLTRFLILRLLGIVYAVAFLVAINQILPLIGSQGLTP
                     IGPFLERVTASTGSTSASFFRLPSIFWFWHSDSALLTVAWIGFILSLAVVAGYANAIV
                     MVVLWLLYMSFCNTGQEWYGYGWEIQLLETGFLAIFLCPLIDPRPFPKRETPIAIIIL
                     FRWLICRIMFGAGLIKLRGDEIWRNSTALYFHFETQPMPGPLSRWFHFLPRVVLRLGV
                     WFNWLAELIAPLFVFWPRIARHIAGVIIVLLQLVLILSGNLSFLNWLTIVPALACFDD
                     GFWARLMPRGLTRMAQRARESAIPSRPMSATSWVVAVIIGMLSFQPVINMLSPDQIMN
                     TSFDPLNLVNTYGAFGSVGRQRYNVVFEGTMDEIPTDSATWKEYPYKGLPDDPDKSPP
                     QVAPYHLRFDWQMWFAAMSTADEYPWTYALVSKLLHNDPGALSLFAANPCPGRPPRYI
                     RAILYHYSFARPGSPGHHWWARQRIGNPWLPAMAIDDPRIRYYLNGSGATP"
     gene            6016..>6242
                     /locus_tag="VKU83_11015"
     CDS             6016..>6242
                     /locus_tag="VKU83_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94136.1"
                     /translation="MKPTVSYRLTLLFIPLLLSANPRPRTKPFFATGSGVSVIAPPRN
                     AGNPPQTADSTYAERLGYPPGARVLIIHVDDA"
BASE COUNT         1361 a         1778 c         1825 g         1278 t
ORIGIN      
        1 cacgcgcggt aagcgccatg aaagttatcg acgggttttg atttcccgtg gacgtcatgc
       61 aggccccgtc ggtaacaaaa acattcttac aggcatggag ttggttccat tcattgagca
      121 tggaagtctt cggatccttt cccatccggc aacctccaac ctcgtggatg tcgagcccgg
      181 ggttctgatg gttatcttcg ccggaaatgt ttttgcagcc ccctgcttcc agcatttccg
      241 caccccgcgc gaggaagtcg gcgcacattt tcgcgtcgtt gtcgtcataa cccaccgagg
      301 tgatgagctg cggaattccc cactgatctt tctgatcgga actgagccgg acatggtttt
      361 caaatttcgg gatcgtctct ccttgcatcc ccatcgacac aaaccagctt ccaggttccg
      421 acagcgaatc cttgtagtcg ccgccaacgc cgtcccggta atcgccgcgt ccccagcccg
      481 cgcgggaagc gccgtagtac gccatatacc cccgcttgaa acccaggcct tcatcgcgaa
      541 atacgtttct gaaggcggga atgaagacct ccgtaggccg gcggccgtaa taataacgat
      601 caggatatcc ttcaaattcg gcgttgaggg aaccgcggta attatggaac gccacataat
      661 ggccaaggat gccgtgatcg ttgccaagcc cgttcgggaa gcgccgcgaa acggaattca
      721 gcaggatcag gttggtattg aggcaggcgg cgtttacaaa gatcacccgc gcgaagtact
      781 cggttgcctt cctggtctgg gcgtcaatca cgcgcacacc caccgccttt cctttcgatt
      841 cgtcgtaaat gatggaatgg acaacgctaa agggctgcag cgtcaacttt ccagtcttct
      901 gcgcccaggg gatcgttgag gaattggaac taaaatagcc cccaaatggg cagccacggt
      961 cacacatgct gcgcgcctgg cattggcccc ggccctgttc aatgtggatg ggctgcggct
     1021 ttgtcagatg tgcacagcgg ccgatgatga ccggccggcc gggataacgg ctgtgaatac
     1081 cctggcgaat atgttcttcg acgcagttca actcccaggg tgggaggaac tcgccgtccg
     1141 gcagattggg cagcccatcc ttattgccgc tgatcccgac gaatcgttcg acataactat
     1201 accatggcgc cacgtcgttg taaccaatgg gccagggtat ggcaatgcca tcgcgcgccg
     1261 gcccttcaaa ttcaaatggg ctccatcgct gcgtctgccg cgcccataac aacgatttcc
     1321 ctccgacctg gtagccgcgg atccagtcga atggcttctc ctggatgtag gggtggaatt
     1381 tgtccggaac aaaaaaatgc tgcgttgcct cgccatacgc atagcatttg cttacgaccg
     1441 ggttttcctc caatgtggcg agcggcatgg cgccacggtg cgcgaacccc cacggaggcg
     1501 ttgttgccgt cggatagtcc ttaaggtgct gaacgggtct tcccctttcg agcatcaggg
     1561 tgtttagacc gtgatcgcaa aattccttgg ccgcccagcc gccgctgatg cccgagccga
     1621 tgacgatagt gtcgtacgta ttcatagaag tcttaaatta ggtcaaagtt ttcgatatga
     1681 gaaaatttat tgccctcctc atcatcgtcg cggatgctct cggaagcttc ggccagggat
     1741 tcgaaacgat aaaggtcgaa ggcggtttga tctccggcgc catggataca aggggagaca
     1801 ttcattcttt taaggggatc ccgtttgcag cgccgcctgt tggcccgctt cggtggaagg
     1861 cgccacagcc tgtagtgcca tggacgggcg tccgctcctg cgaccagttc ggcgcaagcc
     1921 cgatgcagaa caaaccggca gccttcctca tgtggaccga agaatacctg atcgccccca
     1981 cgccaatcag cgaggactgc ctgtacctca atgtgtggtc cggagcgcgg acgccagggg
     2041 agagacgacc tgtattggtt tggatctatg gcgggggatt cagcagcggc ggcggaaatg
     2101 ttccgatata tgacgggacg gcaatggcca gaaagggaat catctttgtc tccatcaatt
     2161 atcgcgtcgg gatttttgga tttttctccg atccggagct gtcgagcgaa tccggccacc
     2221 aggcctcggg caattatggg ctgatggacc agatcgccgc gttaaaatgg gtcaggaaca
     2281 atatcgccgc cttcggcggc gaccccaata acgtcacgat cgccgggcag tccgctggga
     2341 gcatgagtgt gaattgcctg gttgcctcgc cgctcgctaa gggtcttttc caaaaagcca
     2401 tcggcgaaag cgggggtttt ttttcgcggg acatgctgtc gctcggggag gctgaagaaa
     2461 gcggaagaaa gctgatgcaa tccctgggca tcaccggcct cgaagcgatg cgggccatac
     2521 ccgcagacga actgctgaaa aagggagggg ccttacatac ttttccggtg gtcgacaact
     2581 acgtcttacc ggcccaggtc gccgatatct ttgccgcggg aaaagagaac cccgtgtcct
     2641 tgctgaccgg ctggaacgag gatgaaggat tcgtattcgg gccgacgaaa aaggcagccg
     2701 attaccggca ggatatcgag aaggagtatg gcgctgccgc ggcgaccgta ctgaaatatt
     2761 atcccgcgga caacgattca atggcggggg tatcacaggt ccgcctttcg cgggacatga
     2821 tattcggtcg ccagaattat gcctgggcca atgtcgccag tagccatggc cgtcgcgtct
     2881 tcgcctaccg ctttgtccgg cggccgccca ccgccccggg cggccgcaga tggggcgcat
     2941 tccataccgc ggaagtacct tatgcgtatg acaatttgca gttcgtgcac cggccatggg
     3001 agcctttgga ttataatctt gcggacgaaa tgtctaccta ctgggccaac tttgtaaaga
     3061 ccggggaccc gaacggcatt gggctgccgc gatggcccct gtacaccgcg aaggacccgc
     3121 aaatcatggt gttcgggcgt caggccgcag ccggcgtgct ggcggacaaa gctgccctcg
     3181 acttccagct gaccagaatg gaggggagat aatgggaagt atttagaaaa gccttcccca
     3241 atggtttaaa aaccacaaca actcattttt gccggttgtg gattggccgg atcgtgctac
     3301 attcaagctg aaaccactga accatgccct ggacccgtag caccatcctt atcatcgacc
     3361 acgatgatac ggcagccgcc gagcttgccg gtcttctcaa aaaccgtcca caaccacctg
     3421 ctgtattcgt tgcccccgga gtaggaaccg cggagcccgt cctgcgaacg gaatcaatcg
     3481 actggatgtt catccggata attgcctggg atgactttca acggcttcgt cccctcctgt
     3541 tgtcggcgcc gcgccgcgtg gtttttctgt ccggacggaa cgaaaagtgc accgcacatc
     3601 ttccgctcgt actggacagc cacctgcagc caccctaccg gtccagccac ctggtccggg
     3661 cctggaataa atggatggat ccccttttca ttccgcgacc actggacatc ttctttatca
     3721 agagccgcgc gcggttcgaa gccgttcgat acggcgacct gcgccaggtc gaggtcgagg
     3781 acggtaagtt gcgcttatcg acccggcacg cggattacca ggtcacgggc agcctgcagg
     3841 ccttccgcga ccggttgcca atctcgctgg cgctcgtgcg gcgcggatgc ctggtcaatg
     3901 agttttattt gcccgcggag acaaattcgc tgtaccgcgt ttcttcgggg acttccgtcg
     3961 gactttcaaa ttccagggga tagccgtcgg ggtcccgcac tttaaagacc cacatgttat
     4021 tgccaacgaa cggctccgtt acaggcaaat ggcgggcctt aaattcccgg tacagcgcga
     4081 gggcgtcctc gcactgcaca cagatgttca caccgaaggc ttttgtctgg ctggaattcc
     4141 gcagggggtc cgtctcgctc cggggctcct gcagcatcag cgatacgccg tcgcgggtca
     4201 gccaacacca ttcgatcttt ccccgtggag tccattggtg tttcagggta aatcccaatc
     4261 cgcgcacgta aaagtcgagc gacttctcca tatccgtgac accgaagaag gggaccgcca
     4321 gtttgagatt gggagggttc atataccgga agttaacagg ccgcggcgac atcgccaaaa
     4381 tgagttatgg cgtcgccccc gaaccgttca gataatatcg gatcctcggg tcatcgatgg
     4441 ccatcgcagg caaccacgga ttgccgatgc gctgccgggc ccaccagtgg tggcccgggc
     4501 tacctggccg ggcaaaagaa taatgataga ggatggcgcg gatgtagcgg ggtggccggc
     4561 ccgggcaggg attggcggca aaaaggctca gcgcgccggg gtcgttatga aggagtttgg
     4621 atacaagggc atacgtccac ggatattcat cggcggtcga catggcggcg aaccacatct
     4681 gccagtcgaa gcggaggtgg tagggcgcga cctgcggcgg gctcttgtcc ggatcgtccg
     4741 gcaggccttt gtacggatac tccttccagg ttgcggagtc ggtggggatc tcgtccatgg
     4801 tgccttcaaa caccacatta taacgttgac gtccaaccga gccgaaggcg ccgtatgtat
     4861 tgacgaggtt aagtgggtcg aacgaggtgt tcatgatctg atccggtgac agcatattga
     4921 tgaccggttg gaagctgagc attccaatga tcacggcgac cacccacgaa gtggccgaca
     4981 tggggcggga aggtatggcc gattccctcg cgcgttgcgc catgcgcgtc aggccccgcg
     5041 gcataagccg ggcccagaag ccgtcgtcaa aacaggcgag cgccggaaca atggtcagcc
     5101 agttcaggaa ggaaaggttg ccgctgagga tgaggacaag ctgcagcagg acgatgatga
     5161 caccggcgat atgacgggcg atacggggcc agaacacgaa cagcggcgcg atcagttcgg
     5221 ccagccagtt gaaccaaacg ccaagcctca gcacaacacg cggcagaaaa tgaaaccagc
     5281 ggctgagggg tccgggcatc ggctgggtct cgaaatggaa atacagggcg gtggaattgc
     5341 gccagatctc gtccccgcgc agcttgataa gtccggcgcc aaacataatt ctgcagatca
     5401 gccacctgaa caagatgatg atcgctatgg gcgtttcgcg cttgggaaat gggcgtggat
     5461 cgatcagcgg acagaggaag atggcaagaa aacccgtttc aaggagctgg atctcccagc
     5521 cgtagccata ccactcctgg ccggtgttgc aaaacgacat atacagcagc caaagcacga
     5581 ccatgacgat tgcgttggca tatcccgcga caacagcgag ggaaaggata aatccgatcc
     5641 aggcgacggt gagaagagcg gagtcggaat gccagaacca aaatatcgac ggcagccgga
     5701 aaaagcttgc gctcgtcgat ccggttgaag ccgtgacgcg ctcgagaaaa gggccaatgg
     5761 gcgttaggcc ttgcgagccg atcagcggca ggatctggtt gatggctact aaaaaggcta
     5821 ctgcgtagac aatgcccagc aagcgaagaa tgaggaagcg cgtgagccag taggttgggc
     5881 ggtcagccat gacacgcgga ttttgtcctc aaagttcggt gatttagcca taaaaccccg
     5941 gacggaccat cgctccctcc gcgccatggt tcgtccatca ggggttgcgg aattgttaac
     6001 gtatatttaa tgattatgaa accaactgtc tcctaccggc tcactttgct ttttatcccg
     6061 ttgctgctgt cggctaaccc gcggcctcgc acgaaaccgt tcttcgcgac cgggtccggc
     6121 gtaagcgtca tcgcgcctcc ccgcaacgcc ggaaatccgc cccagacggc tgactccacg
     6181 tatgcggaac gcctcggtta ccctcccggc gcgcgcgttc ttatcatcca cgtcgacgat
     6241 gc
//
LOCUS       DATGKE010000652         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_454582, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000652 DATGKE010000000
VERSION     DATGKE010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454582"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="hemA"
                     /locus_tag="VKU83_11020"
     CDS             <1..1137
                     /gene="hemA"
                     /locus_tag="VKU83_11020"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLI94137.1"
                     /translation="DQYEKIVRLAPSFNVTELFVLSTCNRTEIYGFADNSAHLAALLC
                     TQTTGDLGSFMDLAYIRKGEEAIQHLFEVGAGLDSQILGDYEIVGQIKQAVKFARDRQ
                     ATGPFTERLVNCVLQASKAIKNQTGLSGGTVSVSFAAIQYIRERIADPERQRILLIGT
                     GKIGRNTCRNIVDYLGTRNITLINRTESKAAELAAEIGVSHAPVVRIAECVRAADIIL
                     VATNAEQPTLLGSWLEGGKGRKLVIDLSIPCNVEPAAAELPGVTLVNVDELSKIKDET
                     LQKREAEVPKAKAIIAEHLAEWIDWFRMRQHVPVLRAVKSKLKELHTCPVFSRLQGEP
                     FPATDQTDEQIQRVINGMASKMRQHNQGGCHFIEAINEYIAATN"
     gene            1142..2065
                     /gene="hemC"
                     /locus_tag="VKU83_11025"
     CDS             1142..2065
                     /gene="hemC"
                     /locus_tag="VKU83_11025"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HLI94138.1"
                     /translation="MQTPIRIGTRESQLAVWQATQVLQLLEKEGIAARLVPIRSDGDV
                     DLRTPLYEMNVQGIFTRSLDLALLDHQIDIAVHSMKDVPTQLAAGIIQAAVLKRGPFH
                     DLLVYKGSADFLADRNSVAHIATSSIRRRAQWLHRYPAHEIHNLRGNVGTRLRKLAEE
                     QWNAAIFAQAGLERIGLRPENSIVLDWMLPAPAQGAILVACRSDDEETCKRCDALNDA
                     DSALCTKIERDFLRTLLGGCSTPISALAEIEAGDVYFSGQLLSPDGRRNLTVEKILPL
                     EIASDMGRAAAEELLEQGGREIAGGMRVNTP"
     gene            2062..2751
                     /locus_tag="VKU83_11030"
     CDS             2062..2751
                     /locus_tag="VKU83_11030"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLI94139.1"
                     /translation="MSRTTRYLLATGALPPHMAGVAADDVVLEVQSFIAIKPVPAGRL
                     AGLAERRLVAVFTSVNAVEAVLAEVGRPRRWKIYCLGGATYQRLAGELGKACIAGQAD
                     SATALAECIRDRERERQVFFFCGDQRRDELPEILTAEGFEVTETVVYQTVLTPHRLEK
                     SYAGIAFFSPSAVESYFSVNAATNGTVMFAIGHSTADAIRSRCDNQVMICPQPDKPTL
                     IRTMIDYFINK"
     gene            2759..>3420
                     /locus_tag="VKU83_11035"
     CDS             2759..>3420
                     /locus_tag="VKU83_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HLI94140.1"
                     /translation="MSQLRNDLILRVLRGESTERVPVWMMRQAGRYLPDYVKLRNKYS
                     FFERCRTPELAAEITIQPVEQIGVDAAIIFSDILVVPQAMGMEVQLVESKGPLLPEPI
                     RSTRDLDRLLVPDVEEGLGYVFDAIKLTKSYLAGAVPLIGFAGAPWTLLCYMVQGQGS
                     KTFDGAKAFCYMQPDAAHRLLQMITDTTIGYLRGQVRAGADLVQIFDSWGGLLGPDDF
                     ET"
BASE COUNT          764 a          922 c         1065 g          669 t
ORIGIN      
        1 gaccagtacg aaaagattgt caggctggcc ccttctttca atgttaccga gctttttgtg
       61 ctgtcgacct gtaaccggac cgagatctac ggtttcgccg ataattccgc ccatctggct
      121 gcgctgctgt gcacccagac aaccggcgat cttggatcgt tcatggatct ggcgtatatc
      181 cggaaagggg aagaggccat ccagcatctc tttgaagtgg gcgccggtct cgattcgcag
      241 atcctcggcg actatgaaat cgtcgggcag atcaagcagg cagtgaagtt cgcacgcgac
      301 cgccaggcaa cggggccctt tacggagcga ctggtaaatt gcgtactcca ggcgtcaaaa
      361 gcgattaaga accagaccgg cctgagcggg ggaaccgttt ccgtttcctt cgcagccatc
      421 caatatatcc gggaacggat cgccgatccg gaaaggcagc ggatcctgct cattggtacc
      481 ggcaagatcg gtcgaaatac ctgccgcaac atcgtcgact atctcggcac ccggaatatc
      541 accctgatca accggacaga atcaaaggcc gcggagctgg ccgcggagat cggggtcagc
      601 catgcacctg tcgtacgcat cgcggaatgt gtccgcgcgg cggatatcat tctggtggct
      661 acgaatgcgg agcaacctac gcttctcggc tcgtggctgg aaggcggaaa gggcaggaag
      721 ctggtcatcg atctgtcgat cccctgcaat gtcgagccgg cggcggccga attgcccggt
      781 gtcaccctgg taaatgtaga cgagttatcg aagataaagg atgagacttt gcaaaagcgc
      841 gaagctgaag tcccgaaggc aaaggcgatc atcgccgaac atctggccga gtggatcgat
      901 tggttcagga tgcgtcagca tgtcccggtg ctgagagccg tgaaaagcaa attaaaggaa
      961 ttacacacct gccccgtatt cagccggctt cagggcgaac ccttcccggc gacggatcaa
     1021 accgatgagc agatccagcg ggtgatcaac ggaatggcca gcaaaatgcg ccagcataac
     1081 cagggcggct gccattttat cgaggcgata aacgaatata tcgccgctac gaattaatcc
     1141 catgcagact ccgattagga taggcacgcg agaaagtcag ctagccgtct ggcaagctac
     1201 ccaggtcctg cagcttctgg aaaaagaagg gatcgccgcc cggctcgtgc cgatccgcag
     1261 cgacggggat gtcgatctca ggacgccgtt atacgaaatg aatgtgcagg gtatatttac
     1321 ccgcagtctc gatctcgcac tgctggatca ccaaatcgac attgccgtcc attcgatgaa
     1381 agacgtgccg acgcaacttg ccgccggcat catccaggca gcggtcttga agcgcggtcc
     1441 cttccacgat ctgctggtct acaaagggag cgcagacttc ctggcggaca gaaactcggt
     1501 tgcccacatt gcgacgagca gcatccggcg cagagcgcag tggctgcacc ggtacccggc
     1561 gcacgagatc cataacctcc gcggaaacgt gggcacccgg cttaggaaac tggccgaaga
     1621 gcagtggaac gccgccatat ttgcccaggc aggcctggaa cggatcgggc tgcggccgga
     1681 gaacagcata gtgctggact ggatgctgcc tgcgccggcc cagggggcga tcctggtcgc
     1741 ctgcaggagc gatgatgagg aaacctgcaa gcgttgcgat gcgttgaatg atgcggactc
     1801 ggcgctctgc acgaagatcg aaagggactt cttgcggacc ctgctgggtg gctgttcaac
     1861 gccgatcagc gctcttgccg agatcgaagc cggtgatgtc tatttcagcg ggcaattgct
     1921 gtcgccggat ggccgccgga atcttacggt cgagaagatc ctgccgcttg aaatcgcgtc
     1981 cgacatgggc agggccgccg cagaggagct cctcgagcaa ggcggacgtg agatcgccgg
     2041 cggcatgcgc gtaaataccc catgagcagg acgaccagat atttattggc gacgggagcg
     2101 ttgcctcccc atatggccgg agtggcggcg gacgatgtgg tgttggaagt acagtcgttt
     2161 attgccataa agcctgtgcc tgccggccgg ttggcggggt tggcggaacg ccggctggtt
     2221 gccgtgttca ccagcgttaa tgctgtggaa gcggtcctgg ccgaagtggg caggccccgc
     2281 cggtggaaaa tatattgcct cgggggagcg acatatcaac ggctggccgg tgaacttgga
     2341 aaggcctgca tcgcgggaca ggcagattcg gcaaccgccc ttgcggaatg catccgggac
     2401 cgcgaaaggg aacggcaggt attttttttc tgcggtgacc aaagaaggga tgaattgccg
     2461 gagatcttga ccgcggaggg ttttgaggta accgagacgg ttgtatacca gaccgtgctt
     2521 acccctcacc ggcttgaaaa aagttatgcc ggaattgcct ttttcagtcc gtcggcggtg
     2581 gagagttatt tcagcgtaaa cgcggcgacc aatggaacgg taatgtttgc gatcgggcac
     2641 agcacggccg acgcgatccg ctcccggtgc gacaaccagg taatgatctg cccacagccc
     2701 gacaaaccga cgcttatccg gaccatgatc gattatttca tcaacaaata aaaatagttt
     2761 gagccaactg cgaaacgacc tgattttacg agtcctgaga ggggaatcca ccgaacgggt
     2821 tcccgtgtgg atgatgcggc aggcagggcg ttatctgccg gactacgtga agctgcgaaa
     2881 caaatattcg ttcttcgagc gatgccggac accggaactg gctgcggaga tcacgatcca
     2941 gccggtcgag cagatcggcg ttgatgcggc cattattttt tccgatatcc tggtagtccc
     3001 gcaggcgatg ggaatggagg tgcagctggt ggagtcgaaa ggcccgttgc tgcccgagcc
     3061 gatccggtcg acgagggacc tggatcgctt gctggtaccg gatgtggaag aggggcttgg
     3121 ttatgtcttc gacgcgatca aactgacgaa aagctatctt gcgggggccg tgccgctgat
     3181 cggctttgcc ggcgcgccgt ggactttgct atgttatatg gtgcaaggcc agggatccaa
     3241 gacatttgac ggcgccaagg cattttgcta catgcagccg gatgcagcac accggctgct
     3301 gcagatgatc acggatacga caatcggcta tttgagaggt caggtcagag cgggggcgga
     3361 cctcgtccag atcttcgaca gctggggcgg cctgctgggc cccgacgatt ttgaaaccta
//
LOCUS       DATGKE010000653         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_3592, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000653 DATGKE010000000
VERSION     DATGKE010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3592"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VKU83_11040"
     CDS             <1..820
                     /locus_tag="VKU83_11040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLI94141.1"
                     /translation="YLCAMLKSTLIKAAEAGAAVMEHYFQGEFIISNKEGVNNLVTEA
                     DHASEKAIFDVIRHDFPDHFLLSEETGEIVMDSRYKWIIDPIDGTVNFAHGLPICCVS
                     IALEQDGQMVMGAVYNPFLKEFFFAQRGYGATLNDRKIGVSTETSVMKSCLVTGFPYT
                     YLDRANGPLEVFSRLIRAGVPVRRLGSAAMDLCWVAAGRFDGFYEHKLNAWDSAAGYL
                     IVEEAGGRVSDFTGGRYSPYQPHIIATNGRIHDELVAVVNDRHPELSSSINKRI"
     gene            817..1854
                     /gene="thiL"
                     /locus_tag="VKU83_11045"
     CDS             817..1854
                     /gene="thiL"
                     /locus_tag="VKU83_11045"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLI94142.1"
                     /translation="MSESRTEISSLGEFGLIDRLTANIEIKNASTLVGVGDDAAVIDH
                     FGRQTVISTDLLIEEVHFDLVYTPLPHLGYKSVVVNLSDIYAMNAIPTQIVLGLGISN
                     RFSVEALEEFYEGVYAACEAYGVDLVGGDTVSSRKGFIISVTAIGEVAPDRFVRRSTA
                     RKGDLLCCTGDLGAAYLGLILLEREKKIFLEHPQVKADLENEKYVIGRLLKPEARRDL
                     IEFFHDANLTPTAMMDISDGLSSEILHICRQSDLGCVLYEEKIPIAEETRNAAFKFEL
                     DPTACALSGGEDYELLFTVAQEDYEKLVLNEQVSVIGYMTEPEKGRKIITKGGNSFDL
                     TAQGWNAFPSK"
     gene            1856..>3036
                     /locus_tag="VKU83_11050"
     CDS             1856..>3036
                     /locus_tag="VKU83_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLI94143.1"
                     /translation="MKNRVKFICCLPCLILLSCGISRKDFQPDRKYPPAQLQSDYRIF
                     RGTLEAWHPSLYWYTPRDSMNRYFDEGYAALKDSMTEPQFRKILSFVIAKVRCGHTSV
                     GSSKNYAHYLDTAKLKQFPLILKFWADTMVVISNLNRHDSILKRGTLIYSIDGRTARQ
                     LTDTLFPYIVTDGYNRTGKYQYLSSGLHFSSWYKELFGEDSVYHIRYRDTAGIERETE
                     APVYDPRADTVRWSLGRELMPGTGQKRPGTPGRTRPSRRRRKARELLFTRSLDLDTAS
                     HSAFLTLNTFEHGYHLKPFFRTTFRTLHDKKVKNLVIDVRINGGGDAGISTLLTRYLI
                     NKKFKTADSLYTIRRFSRYDGYIQHGLIYDMLSYFTSRRRSDGFYHFRRFEKHYYAPM
                     A"
BASE COUNT          699 a          823 c          874 g          640 t
ORIGIN      
        1 ctatctttgc gccatgctaa aatcgacact aatcaaggcg gcggaggctg gtgctgcggt
       61 gatggagcat tattttcagg gtgagttcat catttccaac aaagaaggcg ttaataatct
      121 ggtcaccgag gccgaccatg cgtcggaaaa ggccattttt gacgtcattc ggcacgattt
      181 tccggatcat ttcctgttga gcgaggaaac cggcgagatc gtgatggatt caagatacaa
      241 gtggatcatc gatcccatcg acggcaccgt caatttcgct catggtctgc ccatctgttg
      301 cgtgtcgatc gcccttgaac aggacgggca gatggtcatg ggggcggtat acaatccctt
      361 tctgaaagag ttcttctttg cgcaacgggg atatggtgcg acgctgaacg acaggaagat
      421 cggggttagc acggagacat ccgtaatgaa gtcatgtttg gttaccggat ttccgtacac
      481 ttacctggac cgggccaacg ggccgctgga agttttttcg cggctgatcc gggccggcgt
      541 acccgtgcgg cgcctcggct ccgcggcaat ggacctgtgc tgggtagccg cgggaaggtt
      601 cgatggcttt tacgaacaca agctgaacgc ctgggacagc gctgccggct acctgatcgt
      661 ggaagaggcg gggggcaggg tatcggattt taccggcggc cgctattctc cctatcagcc
      721 gcacatcatc gccaccaacg gcaggatcca tgacgaactt gtcgccgtgg tgaatgaccg
      781 gcatccggaa ttatcaagta gtatcaacaa aagaatatga gcgaatccag aacggagatc
      841 tcgtcgttgg gggagtttgg gttgatcgac aggctgacgg cgaatatcga gataaagaac
      901 gcctctacgc tcgtcggcgt cggcgacgat gccgcggtga tcgaccattt cggccggcag
      961 acggttatca gcacggacct gctgattgag gaagttcact ttgacctggt ctacacgccg
     1021 ctgccgcacc ttgggtataa atcggtggtg gtgaacctca gcgatatcta cgccatgaac
     1081 gccatcccca cccagatcgt ccttggcctc ggcatcagta accgcttcag cgtggaagcc
     1141 ctcgaagagt tctatgaagg cgtctacgcg gcctgcgagg cgtacggggt ggatctggtg
     1201 ggcggcgaca ccgtgtcatc aagaaaaggt ttcattatct ccgtcaccgc gataggcgag
     1261 gtagcgccgg accggtttgt gcgccggagc actgcccgga aaggagacct gctgtgttgt
     1321 accggcgatc tcggcgcggc ctatctgggg ttgatcctgc tcgagcgcga aaaaaaaatc
     1381 ttccttgaac acccccaggt caaagcagac ctcgaaaacg aaaaatacgt tatcggcagg
     1441 ttgctgaaac cggaggcgcg aagggatttg atcgaattct ttcacgatgc caatcttact
     1501 ccgacagcga tgatggatat cagcgacggc ctcagctccg agatcctgca tatctgccgt
     1561 caaagtgatc tcggctgtgt gctgtatgaa gaaaagatcc ccatcgcgga agaaacccga
     1621 aacgccgcat tcaaattcga gctcgatccg acggcctgcg cgctaagcgg cggcgaggac
     1681 tatgaactgt tgtttaccgt cgcccaggag gactacgaga agctggtatt gaacgagcag
     1741 gtcagcgtga tcgggtacat gacggagcct gaaaagggca gaaagatcat cacgaagggc
     1801 ggcaacagct ttgaccttac ggctcagggc tggaacgcct ttccttccaa ataacatgaa
     1861 gaaccgagtg aaattcattt gttgtctacc ctgtcttatc ctgctgtcct gcggaatttc
     1921 ccggaaggat ttccagccgg accgcaagta cccgccggcg cagctgcaat cggactatcg
     1981 tattttccgg ggcacgctcg aagcctggca tcccagtctc tactggtata cgccaaggga
     2041 cagcatgaac cgttatttcg acgagggcta cgcggcgttg aaagactcga tgaccgaacc
     2101 ccagttcaga aagatcctga gctttgtaat cgccaaggtg cgttgcgggc acacgtctgt
     2161 ggggtcatcg aagaattacg cgcactatct ggacacggcc aaactgaagc agtttccgct
     2221 catcctgaaa ttctgggcgg ataccatggt ggtgatcagc aatctgaacc ggcatgattc
     2281 catcttgaaa cggggtacgc ttatttacag catcgacggg cggacggccc ggcagctgac
     2341 cgacaccctt ttcccatata tcgtcaccga tggttacaac cgcacgggaa aatatcaata
     2401 cctcagctcg gggcttcatt tttcgtcatg gtataaagaa cttttcgggg aagactcggt
     2461 ctaccatatc cggtatcggg acacggcggg tatcgaaagg gagacggaag cgccggtcta
     2521 tgacccgcgg gccgatacgg tgcgctggag cctgggccgg gagctcatgc cgggaacggg
     2581 tcagaagcgg cccggcacgc ccgggcggac gcggcccagc cgcaggcgcc ggaaagcgag
     2641 ggagctgctg ttcacgcgat ccctcgatct cgacacggca agccattccg ccttcctgac
     2701 gctgaatacg tttgaacacg gataccacct caagccgttc ttccgaacga cattcaggac
     2761 gctgcacgat aagaaagtga aaaacctggt catcgacgtt cggataaatg gcgggggaga
     2821 cgccggcatc tcaaccttgc tgacccggta cctgatcaat aaaaagttca agaccgccga
     2881 ttccctgtac acgatccggc gcttcagccg ttacgacggt tatatccagc acggcctgat
     2941 ctacgatatg ctgagctatt tcacgagccg gcgccgctcc gacgggtttt atcactttcg
     3001 ccgtttcgag aagcactatt acgcacccat ggccag
//
LOCUS       DATGKE010000654         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_249218, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000654 DATGKE010000000
VERSION     DATGKE010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249218"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2554
                     /locus_tag="VKU83_11055"
     CDS             <1..>2554
                     /locus_tag="VKU83_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLI94144.1"
                     /translation="YTIIPRIRKQIVFAKHNIIKDPPFIKNDLVSCRNMLIYVSPALQ
                     QRIFALLLFAAGREGFIFLGPSENSALLKKDVKEISGRWKIYRKTAESRPGYYYQQGI
                     PESYGSPLLHPSRVAQPKETKSQSLLWDDFRQVILRDLCYVVLYIDSNFDIQETIGPY
                     EKFLELPRKQLHLNLLRMMPSGLSTLLSAETRKAEKTGRKAHLPNIRYKKGNETITVE
                     AFIRPGGKYNDGLPTMIALREVSAMTTKGAVNHNSNGNNQNSDQYVEALEAELSDTRN
                     QLQLAIEDLETTNEELQSTNQELLSANEELQSSNEELQSLNEELITLNTEHQLKIKEL
                     LELNDDLNNYFRSTDIAQVFLDNQLRIRKFNAASARMINFIDTDMGRPLTHISNNIRS
                     ENLLKDVQSVQSTGRPIEKEVQMNTGKNLLMRIMPYLTHDGKREGVTIAFVDITAITD
                     LNNIIRAVFNSSPSAIFAFQARRDFEGQVTDFTVLSANYAANAMCRRSNDDLRTMSLK
                     KDLPQLAAYDLLTKYLDVLRTDKAYHGSVLFREENQWYDVTIVKMMDGFVATFTNITE
                     RKTAEDKLRVSYAELLSARENLKELNSELENKVKERTRALALSEERFRLVARVTNDAL
                     WDWEFAANLVWWSDTFYQQFGHSKTDGPLDRQGWLERVHPLDRAAVEKSITAAIRNQQ
                     PQWTHEYRFLKENGDYAHVLDRAQILRDDNGTPYRMLGSMLDITELKRASEEIARAKE
                     VLEEKVAERTRQLQLLNDALETSNHDLQQFASIASHDLQEPLRKIHMFSRAIMDKESD
                     KMTEEMKTHFDKIVRSTDRMRALIIDILNFSRLSAEVPHFKRTDTREMVADIL"
BASE COUNT          663 a          775 c          637 g          479 t
ORIGIN      
        1 ctatacgatc attccccgca tccgcaaaca gatcgtcttc gccaaacaca atatcatcaa
       61 ggatccgccc tttataaaga acgacctcgt cagttgccgg aatatgctga tctacgtcag
      121 ccccgctctg caacaacgca ttttcgccct gctgctcttt gcagccggcc gggaagggtt
      181 catcttcctt ggcccaagcg agaattcggc gctcctgaaa aaggacgtaa aggagatcag
      241 cggccgctgg aagatttatc gcaaaaccgc cgaatcgcgg ccggggtatt attaccagca
      301 aggcattccg gaaagctatg gttcgcctct gctgcaccca tcccgcgtcg cccagcctaa
      361 ggaaaccaag tcacaaagcc tgctctggga cgacttccgc caggtgatcc tcagggacct
      421 ctgttacgtg gtgctatata tcgattcgaa cttcgacatc caggaaacga tcggcccgta
      481 tgaaaagttc ctggaacttc cccgcaaaca gctccatctc aacttgctgc gaatgatgcc
      541 ttcaggcctg tccacgctgt tgagcgctga gacccggaag gccgagaaaa cgggccggaa
      601 agctcacctc cccaacatcc gctataaaaa aggcaacgag accatcaccg tcgaggcctt
      661 catccggccg ggcggcaagt ataacgacgg gctgccgacc atgatcgccc tccgggaagt
      721 atccgccatg actacgaaag gtgccgtcaa ccataacagc aacggaaaca accagaatag
      781 cgaccaatac gtcgaggccc tggaagcgga gctgagcgat acccgcaatc agctccagct
      841 cgccatcgaa gacctggaaa cgaccaacga agaattacaa tccacgaacc aggaactcct
      901 ttccgcgaat gaggagctcc aaagctccaa tgaagagctg caatcgctga acgaagaact
      961 gataaccctg aacaccgagc accagctgaa gatcaaagaa ctcctggagc tcaacgacga
     1021 cctcaataac tatttccgca gcaccgacat cgcccaggtc ttcctcgaca accagcttcg
     1081 gatccgaaag ttcaacgctg cctctgcccg gatgatcaat ttcatcgaca ccgatatggg
     1141 ccggccgctg acgcatatct cgaataatat ccggtcggaa aacctcctga aggacgttca
     1201 gtccgtccag tcgaccggtc gcccgatcga gaaagaggta cagatgaata ccggcaagaa
     1261 cctgctcatg cggatcatgc cctatttgac gcacgacggg aagcgcgaag gcgtaaccat
     1321 cgcgtttgtc gacatcaccg ccatcaccga tttgaacaat attattcgcg cggtcttcaa
     1381 ctcgagcccc agtgccatct tcgccttcca ggcacggcgt gatttcgaag gccaggttac
     1441 cgatttcacc gtgctgtcgg ctaactatgc ggccaacgcc atgtgcaggc gcagtaatga
     1501 cgacctcagg accatgtcac tgaagaaaga cctgccccag cttgctgcct atgacctgct
     1561 gaccaaatac ctggacgtgc ttcgcaccga caaggcgtac cacggttcag tcctcttccg
     1621 cgaggagaac caatggtatg acgtcacgat tgtgaagatg atggacgggt ttgtcgccac
     1681 tttcaccaat attactgaac gcaaaacagc cgaagataag ctgcgggtca gctatgccga
     1741 actgctcagc gcccgtgaga acttgaaaga gcttaattcg gaactggaaa acaaggtcaa
     1801 ggaaaggacc cgtgccctcg cccttagcga agagcgcttc cgcctcgtcg cccgcgttac
     1861 caatgatgcc ctttgggatt gggaattcgc ggccaatctt gtatggtgga gtgatacttt
     1921 ttaccagcaa ttcgggcaca gtaagaccga cggcccactc gaccggcagg ggtggctgga
     1981 gcgggtgcat cccctcgatc gggctgccgt cgaaaaaagt attaccgcag ctatccggaa
     2041 ccaacagccc cagtggacac atgaataccg gttcctcaag gagaacggcg attatgccca
     2101 cgtcctcgac cgcgcccaga tcctccgtga cgacaatggc actccatacc gcatgctcgg
     2161 ctccatgctc gatatcacgg aactgaaacg ggcgagcgag gaaatcgccc gcgccaaaga
     2221 ggtcctggag gagaaggtgg cggaacgcac caggcagctc caactcctga atgacgctct
     2281 ggaaaccagc aaccacgacc tgcagcaatt cgcatcgatc gcttcccacg acctccagga
     2341 gcccctgcga aagatccata tgttttcgcg ggcgataatg gataaggaga gtgacaagat
     2401 gaccgaagaa atgaagacgc atttcgacaa gatcgttcgt tcgaccgacc gcatgcgcgc
     2461 gctcatcatc gacattctga atttctcccg gctctcggcc gaagtaccgc atttcaaacg
     2521 aaccgatacc cgcgagatgg ttgccgatat cctg
//
LOCUS       DATGKE010000655         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_930328, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000655 DATGKE010000000
VERSION     DATGKE010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930328"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VKU83_11060"
     CDS             <1..568
                     /locus_tag="VKU83_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLI94145.1"
                     /translation="ILLVAGYIGYRNWIKEPNERHALEAMFRAEEYYRMDSARLALNG
                     DNINYGFLKVIARYGGTRAANLAAFYAGSCYMKMGDFNNAIKYLKDFSTSVEILQERD
                     YGLLADAYSELNRKQEAAEQYKKAGTYYEKDELFSPEYLFRSGYLYESMGKTQDAIAM
                     YRIIKDKYPQSARGVEIDKYLARLGALQ"
     gene            582..1076
                     /gene="ribH"
                     /locus_tag="VKU83_11065"
     CDS             582..1076
                     /gene="ribH"
                     /locus_tag="VKU83_11065"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLI94146.1"
                     /translation="MAQVDKSNLFKTAAEIPNLTGAAVVIVRTEWNDAIVSALETGCC
                     EILRKANVLFRIVTVPGAFEIPFAIRAYWEAHKYKDDRPAAFIALGCVLRGDTPHFEY
                     VCRAVTDGVVKLNLTLPIPTIFGVLTVDNQQQAEERTGGKQGNKGQEAAVTALKMISL
                     QKTL"
     gene            1076..1795
                     /locus_tag="VKU83_11070"
     CDS             1076..1795
                     /locus_tag="VKU83_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLI94147.1"
                     /translation="MTIQLFIPCFVDQLYPQTAFNMVKVLEKAGVKVDYNTDQTCCGQ
                     PAYNAGFRNDAREVCSKFVKDFSGADYVVAPSASCVGFVRNYYSTLFDNSSLHNDVRD
                     LGKRIYEFSDFLVNILGVDNVGASLYGKATYHDSCAALRECRIRQEPRRLLANVKGLS
                     LVEMADTETCCGFGGSFAVKFESISIAMADQKVNNALATGADFIISTDLSCLMHLDGY
                     IRHKGYPIKTMHIADVLANNS"
     gene            1823..2233
                     /locus_tag="VKU83_11075"
     CDS             1823..2233
                     /locus_tag="VKU83_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLI94148.1"
                     /translation="MAHWLVKSEPSVYSWDQLVKDKLTYWDGVRNYAARLHLRGMKKG
                     DEVFYYHSNEGTEIVGIARVVKEAYQDPTTDDDAWVAVDLQPVRRLKNPVSLNQIKAD
                     KRLSEMALVRIGRLSVQPVTDREWKIILEMAGEK"
BASE COUNT          565 a          626 c          645 g          500 t
ORIGIN      
        1 catactcctg gtcgcgggtt atatcggcta ccgcaactgg atcaaggagc cgaatgagcg
       61 gcacgctttg gaggcgatgt tccgcgccga agaatactat cggatggatt ccgcccggct
      121 ggcccttaac ggtgacaaca tcaattacgg ctttcttaag gtgatcgccc gatatggcgg
      181 cacccgggcg gcgaacctgg ctgcgtttta tgccgggagc tgttatatga aaatgggcga
      241 cttcaacaat gcgatcaaat acctgaagga cttttcgact tcagttgaaa tcctgcagga
      301 gcgcgattat ggcctgctcg ccgatgccta ttcggaattg aacagaaagc aggaggctgc
      361 cgaacaatac aaaaaagccg gcacctacta tgagaaggac gaattgtttt cgcccgaata
      421 cctttttcgt tccggttacc tgtacgagag catgggcaag acccaggatg cgatcgccat
      481 gtaccgcatc ataaaggaca agtatcccca gtcagcccgc ggcgtcgaga tagacaaata
      541 tcttgcccgg ctgggcgctt tacaataaca aggaaactgc catggctcag gtcgacaaaa
      601 gcaacttatt taagaccgcc gctgagatcc cgaacctgac cggagccgct gtagttatcg
      661 tccggacgga atggaacgat gcgatcgtca gcgcgctgga aaccggatgc tgcgaaatcc
      721 ttcggaaggc caatgtcctg ttccggattg tgacggtccc gggcgccttt gagatcccct
      781 ttgccattcg tgcttactgg gaagcacaca aatacaagga tgaccgccca gcggcattta
      841 tcgcgcttgg ctgcgtgctt cgcggggata cgccccactt tgaatatgtc tgcagggccg
      901 ttaccgacgg tgtcgtgaag ttaaacctga cccttcccat tccgacgatt tttggggtgc
      961 tcacggtgga caatcagcag caggcagagg aacgaacagg cggtaaacag ggcaacaaag
     1021 gacaggaggc agctgtcacg gcgctaaaaa tgatatccct gcaaaagaca ttgtagtgac
     1081 catccagttg ttcataccgt gtttcgtgga ccagctgtat ccccagaccg cattcaatat
     1141 ggtgaaagtg ctggaaaagg ccggagtgaa ggtcgactac aatacggatc agacctgctg
     1201 cggacagcct gcctataacg ctggtttccg caacgatgcg cgggaagtct gttcgaaatt
     1261 cgtaaaggat ttttccggcg ccgattatgt tgtggctccc agcgcaagct gtgtcggctt
     1321 tgtgcgcaat tactactcga cgctcttcga caactcctcc ctgcacaacg atgttcgcga
     1381 tcttggcaaa aggatctatg aattttccga cttccttgtt aacatcctgg gcgttgacaa
     1441 cgtcggcgca agcctatatg gcaaggctac ctatcatgac tcgtgtgccg cccttcgtga
     1501 atgcaggatc aggcaggagc cgcgccggct cctggccaac gtaaaaggat tgagcctggt
     1561 ggagatggct gatacggaga cctgttgcgg ctttggaggc agctttgccg tcaagttcga
     1621 atccatatcg atcgccatgg ctgaccaaaa agtcaataat gccctggcga ccggcgccga
     1681 ttttatcatc tcgacagacc tgtcctgcct catgcacctg gacggctaca tccgccataa
     1741 ggggtacccc ataaagacca tgcacatcgc cgacgtcctc gcgaacaaca gctgaggaag
     1801 tgttggatta aaaaaaattg ctatggcaca ttggctcgtt aagtcagaac cctccgttta
     1861 cagttgggat cagttggtga aggacaaact cacctattgg gacggcgtcc gaaactatgc
     1921 agcccggctc cacctgcgcg gcatgaaaaa gggcgacgaa gttttctatt accacagtaa
     1981 cgagggcacc gagatcgtgg gcatcgccag ggtcgtcaaa gaggcgtacc aggaccccac
     2041 gaccgacgac gacgcatggg tagcggttga cctgcaaccg gttcgccggc taaagaaccc
     2101 ggttagtctc aaccagatca aggccgacaa gcggctttca gagatggcgc tcgtccgcat
     2161 cggccggctt tccgttcagc ccgttacgga ccgggaatgg aagatcatcc ttgaaatggc
     2221 aggtgagaaa tagcatgccg gtggcggcgg aatagtgcat gaggtgtagc caggagcctt
     2281 acaccgcccc cccgggtggg ctccttttct taattgggcc gaattcggcc caatta
//
LOCUS       DATGKE010000656         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_614337, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000656 DATGKE010000000
VERSION     DATGKE010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614337"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VKU83_11080"
     CDS             <1..190
                     /locus_tag="VKU83_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI94149.1"
                     /translation="PLLDGFACTRKIRKHFKDIPIIAVTASPKNEILTEIMACGMNDF
                     VSKPFKPNELRAKILEYL"
     gene            complement(250..744)
                     /locus_tag="VKU83_11085"
     CDS             complement(250..744)
                     /locus_tag="VKU83_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLI94150.1"
                     /translation="MLKIGLLSDTHSYLDDAIFRHFGTCDEVWHAGDFGNAELARRLA
                     DFRPLRGVCGNIDGPDIRAGFQDRLRWQSEGIRVFMTHIGGYPPRYNPTVLQELRREP
                     ADLFVCGHSHILKIMYDEKLKCLYMNPGAAGRQGWHQVRTLIRFAIDGSEMKDCEIIE
                     LGNR"
     gene            complement(761..1534)
                     /locus_tag="VKU83_11090"
     CDS             complement(761..1534)
                     /locus_tag="VKU83_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94151.1"
                     /translation="MSFYYWHPGFGLDSLQDTTLRRNGVRTLYIRYFDVDWLPADSAP
                     VSLFTTDYRNAAPTGDVIPVISIRDRVFDRLTPATIPGFAAGIFSRVRSINASRRLQN
                     REIQFDCDWSEKSQRNYFFFLQEYHRISVQAVSAAIRMNQLRYPDRAGIPPVDYGVLF
                     YFNLQETDTGDVQSLFERPVAHRFTPSLRSYPLTLDIALPLFGSGKSRDGEELFDIIS
                     DVNRHSNHHIRNLIFFNLDDRNLLRYDPRVFKEALARVD"
     gene            1859..3016
                     /locus_tag="VKU83_11095"
     CDS             1859..3016
                     /locus_tag="VKU83_11095"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HLI94152.1"
                     /translation="MPHYFSLGTVPHKRHTQFRRQDGSLYSEQLFSTEGFSNDYSLLY
                     HCHPPTEIIGTDEAYDVQPRVAEERMLKHRSFEGFRLRPEEDYLRSRKPVLVNADCHI
                     VLAAPQRSMTDYFYKNADADEIIFIHRGTGTLETMYGDLSFGYGDYLVVPRGTVYRIR
                     LDNEDNRLFIVESFSPVRYPRKYLSPYGQLLEHSPYCERDIRGPQSLTTHDQKGDFLI
                     RTKKKGRIYGIHYGHHPFDVVGWDGCCYPFAFSIHDFEPITGRVHQPPPVHQTFEGRN
                     FVVCSFCPRLFDYHPLAIPAPYNHSNIDSDELLYYVDGDFMSRKNVTKGMITLHPGGI
                     PHGPHPGAVQRSIGARETKELAVMVDTFHPLMVTEDALEIENPGYTMSWAE"
     gene            3166..3582
                     /locus_tag="VKU83_11100"
     CDS             3166..3582
                     /locus_tag="VKU83_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94153.1"
                     /translation="MIQFHELRIGDLVLVEYNGQRLEGEIIGLNGDEKEIDVRTTAQD
                     FWYSPNDLYGIAVDDNQLMQLGFERQELPDGRVKYLKGPFRIMLSQKGNFSDFEMWYR
                     EDRRHITRELAVHELQNHYHQMTKVDLARPSDLAIH"
     gene            3715..3933
                     /gene="infA"
                     /locus_tag="VKU83_11105"
     CDS             3715..3933
                     /gene="infA"
                     /locus_tag="VKU83_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLI94154.1"
                     /translation="MAKQALIKQDGIILEALSNAMFKVKLENGHEILATISGKMRMNY
                     IRILPGDKVGVEMSPYDLTRGRIIFRYK"
     gene            3940..4056
                     /gene="ykgO"
                     /locus_tag="VKU83_11110"
     CDS             3940..4056
                     /gene="ykgO"
                     /locus_tag="VKU83_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HLI94155.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYVINKKNPRFKQRQG"
     gene            4084..4464
                     /gene="rpsM"
                     /locus_tag="VKU83_11115"
     CDS             4084..4464
                     /gene="rpsM"
                     /locus_tag="VKU83_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLI94156.1"
                     /translation="MARIAGVDLPKNKRGEIGLTYIYGIGRSSAQHILNSAGIDLDKK
                     VNQWNDEELNAIRNIITNEFKVEGALRSEVQMNIKRLLDIACYRGLRHRKGLPVRGQR
                     TRTNSRTRKGKRKTVAGKKKVAKK"
     gene            4686..5090
                     /gene="rpsK"
                     /locus_tag="VKU83_11120"
     CDS             4686..5090
                     /gene="rpsK"
                     /locus_tag="VKU83_11120"
                     /inference="COORDINATES: protein motif:HMM:NF003698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLI94157.1"
                     /translation="MAKAQASTAKTAAKKRIVKVDSYGDAHITASFNNIIISLTNKNG
                     QVISWSSAGKMGFKGSKKNTPYAAQMASSDAAKTALDAGLKKVDVYVKGPGSGRESAI
                     RALANNGIEVVMIKDVTPLPHNGCRPPKKRRV"
BASE COUNT         1353 a         1272 c         1496 g         1124 t
ORIGIN      
        1 gccgttgctg gatggatttg cctgtacccg gaagattcgc aaacacttca aagacatccc
       61 catcatcgcg gttaccgctt cgccgaaaaa tgagatcctc accgagatca tggcgtgcgg
      121 catgaacgat ttcgtcagta agcccttcaa accgaacgag cttcgcgcca agatcctcga
      181 atacttgtaa acggctgtcc attccggcag accgcttccc gggcagcggg ttgaccgggt
      241 aagcggcgat tatcgattgc ccagttcaat gatctcgcaa tctttcatct cgctgccgtc
      301 gattgcgaaa cggataagcg tacgcacctg gtgccaaccc tgccggcccg ccgcgccggg
      361 attcatgtag aggcatttta acttttcatc gtacatgatc ttcaggatgt gggagtgtcc
      421 gcagacgaac aggtccgccg gttcccttcg gagttcctgg agtacggttg ggttatagcg
      481 cggaggatag ccgccgatat gggtcatgaa tacccggatg ccttccgact gccaccggag
      541 tctatcttga aagccggcgc ggatatccgg gccgtcgatg ttcccgcaga cgccgcgaag
      601 aggccgaaaa tccgcgagac ggcgggcgag ttccgcgttg ccgaagtcgc cggcatgcca
      661 gacctcgtcg caggtcccga aatgacggaa gatggcatcg tcgagatagc tgtgcgtgtc
      721 cgaaagcagg ccgatcttta acatggcgtt ggcttggccc ttagtcgacc cgggctagtg
      781 cttccttaaa gacccttggg tcgtagcgca acaggttccg gtcgtccagg ttaaaaaaaa
      841 tgagattgcg aatatggtgg ttggagtggc ggttcacatc cgaaatgatg tcgaagagct
      901 cctctccgtc ccgggatttg ccggatccaa agaggggaag cgcaatgtcg agggtgagcg
      961 gataagaccg gagagagggg gtgaaacgat gcgcaacggg ccgttcgaaa agggactgta
     1021 catcgccggt gtctgtctcc tgcaagttga aatagaaaag aacgccatag tctaccgggg
     1081 ggatgccggc gcgatcgggg tatcgcagct ggttcatgcg aatggcggcc gaaaccgctt
     1141 gtaccgatat gcgatggtac tcttgcagga agaagaaata attccgctgc gacttttcag
     1201 accaatcgca gtcaaattga atttcccggt tctgaaggcg gcgggaggcg ttgatgcttc
     1261 tgacgcgtga aaaaataccc gcagcaaatc cggggatcgt cgctggcgta agcctgtcaa
     1321 acacgcgatc gcggatggag atcaccggga taacgtcacc ggtgggagcc gcattccggt
     1381 agtctgtggt gaagagggaa acgggcgcgg aatccgccgg aagccaatcg acatcaaagt
     1441 agcggatata cagggtgcgg acaccgttac gccgtagggt agtgtcctgc aggctgtcga
     1501 ggccgaagcc cgggtgccag taatagaagc tcatggtttt tgtccgctgc tggcaggagg
     1561 tcaggtacca ggcaagacat aggcaaatca ttcgaatatt tcggacaggc accgcggaaa
     1621 agaattttgt ataaaggtaa caggaataac agcatgacag gaagatttat taactttacg
     1681 cagaaggttg catatgccat tctttcgaaa tttcacctac tggatcgata agcgcaattg
     1741 gaaatattat tttcttatgg ttgcgctgga gatgggctcc gggccacggg tatacatgta
     1801 cgggtgacag ccgctcagcc ctgaaaattc atttttttac attttaaaat cccgatccat
     1861 gcctcattat ttttcattgg ggacggtccc acataagcgt catacgcaat ttcgccggca
     1921 agacggctcc ttatattccg agcaattatt ctcgacggaa ggtttctcca acgattattc
     1981 cctgttgtat cactgtcatc cgccaaccga gatcatcggg accgacgagg cgtatgatgt
     2041 gcagccccgg gtggccgagg aaaggatgct gaagcaccgg agttttgaag gttttcgcct
     2101 ccggcccgag gaagactacc tgaggagcag gaagcccgtg ctggtcaatg cggactgtca
     2161 tatcgtcctg gcggccccgc agcggagcat gaccgattat ttttacaaga atgccgacgc
     2221 cgatgagatc atctttattc accgggggac cgggacattg gagacaatgt acggggactt
     2281 gtccttcggc tatggcgatt acctggtcgt accacgggga acggtctacc ggatccggct
     2341 cgacaacgag gataaccggc tattcatcgt cgaatcattc agtccggtca ggtacccaag
     2401 gaaatatctc agcccatacg ggcaattgct cgagcattca ccttattgcg agcgcgatat
     2461 tcgcggtcct cagtcgctta ccacacatga ccagaagggc gacttcctca tccggacaaa
     2521 gaaaaaaggg cgcatttacg ggatccacta tggtcatcat ccattcgatg tggttggatg
     2581 ggacggctgc tgctaccctt ttgctttcag catccatgac ttcgaaccga tcaccgggcg
     2641 ggtgcaccag ccgccgcccg tacatcagac ctttgagggc cgcaactttg tcgtgtgttc
     2701 gttttgtccg cggttatttg attatcatcc gcttgcaatc cccgcgcctt acaaccatag
     2761 taatatcgac agtgacgaac tgctttacta tgtggatggc gatttcatga gcaggaagaa
     2821 cgtgacgaag gggatgatca ccttacaccc gggaggaatt ccacacggac cgcatcccgg
     2881 tgcggttcaa cggagtattg gggcgaggga gacaaaggaa ttggctgtga tggtggatac
     2941 attccatccg ctaatggtga ccgaggatgc actggagatc gagaacccgg ggtataccat
     3001 gagctgggcc gaatagggca ggccggtttc atccggctgg gcaaaccgga tgtgcatccg
     3061 ggcgggatgc cttggcatgc ccaaaaaagc gcgacgaacc cttatggcgc attggaaatg
     3121 ggcgggaaat accgtaaatt ggggaaatca aaaaaacgat tccatatgat ccagtttcac
     3181 gaactaagga taggggacct tgtgctggtg gaatataacg gccagcgcct ggaaggggaa
     3241 atcatcgggc tgaacggaga tgagaaggag atcgatgtgc ggacgactgc ccaggacttc
     3301 tggtatagtc cgaacgatct gtacggcatt gcggtggacg acaaccagct tatgcaactt
     3361 ggattcgagc ggcaggaatt gccggacggc cgggtcaagt acctgaaggg cccgttccgg
     3421 atcatgcttt cccaaaaagg gaatttttcc gatttcgaga tgtggtatcg ggaggaccgg
     3481 cgacacataa cgcgcgagtt ggcggttcat gagcttcaga accattatca ccagatgaca
     3541 aaggtcgacc tggcgcgccc atctgacctg gcgatccatt agggccgtca aatataaaaa
     3601 tactttattt ttgcagattc cgggtttcct gccggggcat cggttttcgg tggggtagga
     3661 catatatttt gatgtaattc aaattattcc tatctttgcg ctcccaaaaa ctgtatggcg
     3721 aaacaagcac tcatcaaaca agatggtatt attctggaag ccttgtccaa tgctatgttc
     3781 aaggtaaagc tggaaaatgg tcatgagatc ctggccacca tctctgggaa aatgaggatg
     3841 aattatattc ggattttgcc gggcgacaag gttggggtgg aaatgagccc ctatgatctg
     3901 accaggggaa ggataatatt caggtataaa taaacaacaa tgaaagttcg tgcatccatc
     3961 aagaagcgca gtgccgattg taagatcgtg aggaggaagg gcaagcttta cgtcatcaac
     4021 aagaaaaatc cgcggttcaa acaacgtcag ggctaaaacg aaaaacaaca gaaaaacaag
     4081 aatatggctc gtattgccgg tgtagactta ccaaaaaata agagaggaga gatcggtctg
     4141 acctatattt atggtattgg ccgatcgtcc gcccagcaca ttcttaactc tgccgggatc
     4201 gatctcgaca agaaggtaaa ccagtggaat gacgaagagc tgaatgccat ccggaacatc
     4261 atcaccaatg agttcaaggt ggaaggcgcg ttacgatctg aggtccagat gaatatcaag
     4321 cgattgctgg acattgcctg ttaccgcggt ctgcgtcaca ggaagggctt gccggtgcgc
     4381 ggccagcgga cccgcaccaa cagccgtacc cggaaaggca agcggaagac ggtggccggc
     4441 aagaagaaag tggctaagaa gtaaagattc gatcctggcc ggcggccgca ttggcggaag
     4501 ccggacagtt ttgataaatg caccatgagc gggccgaaag cccgtgaaat gaccggatca
     4561 gctgcggatg cccggtgcag gcggagggtt gatccaatcc cggtaaaacg ggatcatcag
     4621 tggacgacgg ggaagaatcc ggcgacgcta aattgtaaaa acaaagacta cctcatttaa
     4681 agcgtatggc aaaagcacag gcaagcaccg cgaagacggc tgccaaaaaa aggatcgtca
     4741 aggtggatag ttacggggat gcccatatca ccgccagttt caacaacatt atcattagtc
     4801 tcacgaacaa gaacggccag gtaatctcct ggagcagtgc gggaaagatg ggtttcaagg
     4861 gatccaagaa aaacaccccc tacgccgctc agatggcctc gtcggacgca gccaagacgg
     4921 cattggatgc gggcctgaaa aaggtcgatg tgtatgtgaa aggcccaggc tcgggccgcg
     4981 aaagcgccat ccgcgccttg gccaataacg gtatcgaggt ggtcatgatc aaggatgtga
     5041 cgccgctgcc gcataatggc tgccgtccgc cgaaaaagcg cagggtttaa aatcaaacaa
     5101 taaagccggg taagcttcaa ttttaagatt tttagctaag cttatgccgg gaacttaaaa
     5161 atctaaccgt tacattatgg cacgttacac cggtccgaag accagaattt cccgcatttt
     5221 tggcgagccc attttaggga atggg
//
LOCUS       DATGKE010000657         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_376770, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000657 DATGKE010000000
VERSION     DATGKE010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376770"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1086
                     /gene="traN"
                     /locus_tag="VKU83_11125"
     CDS             205..1086
                     /gene="traN"
                     /locus_tag="VKU83_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraN"
                     /protein_id="HLI94158.1"
                     /translation="MKTLISAILLFTLTLTAHLSTHSQSLTLPPSTLILHYRLALSYS
                     STTVLLFPAPVRPVDRGDRDVLAQRQTGVENVLKLKAARRNFSPTNLHVFTADGRVYA
                     FDLYYTDTLASTYNLTRLDPGTISSGPEIVLAGQALNTEQLNACVDLVRRQPPKFSIR
                     IHRFRMRLQLQDIDMAGPILFFRFAVSNKSDLDYTPDFLRLYLSDEARAKRTSRQDRE
                     LTPLYADTLVTIPGRQRRIFIVGLPRLTIPDRKEFRLELYEKNGGRTLALRIRNRQLL
                     RALRLAVPTSPSSNANQ"
     gene            1073..2158
                     /locus_tag="VKU83_11130"
     CDS             1073..2158
                     /locus_tag="VKU83_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94159.1"
                     /translation="MPTNNQPFDRVLDSLLMQAEQEAIQRTRNPTLRRDDPYLLGLRQ
                     AQYESIIDGYRNRDDLTRDEKRGLTYVRTQLRRINARLHPNLLSRIRYLRPVNWVFNW
                     IRGRAALIEGYNNQLQDQDRRTIQEQNVQRLSEEMKKAGFKVIPEAALKRAIAQDRPY
                     FNIPYAASDDAKTDYVLHFKKLPGTDAYYLDNFDAATKPTPQEVLRGNPIVRQNFSLI
                     DDYSLSAHEAASVIRQIPVARNIDGQDRWLITDPLGERRWVEFDLQAALNKLPIKELR
                     DPARMQALVTALHAGSSREVTFRKNNQTHELRVQVDSWAEGLLFKDKNGNLVDVDRIF
                     QSNANVQKVLSMAQDEAVVRDLHPRRS"
BASE COUNT          566 a          721 c          501 g          510 t
ORIGIN      
        1 agcgtcagta gcctcggcct cacctctttt gatccctcac tgggcgcaca ggccgccgcc
       61 gcagggatcg aaactgccag gaacctgatc ggccgcaaga tccgactcat caccgccacc
      121 cttaaggcag gcaccctcgc catccttcaa accggccaat ctcacccatg atcccaatta
      181 tctcatcccc ccaacgtaca attcatgaaa acgctcattt ccgccatcct ccttttcact
      241 ctcactctca cggcccactt gtctacgcat agtcagtctt tgactctccc gccgtccact
      301 ttaatcctcc actaccgact agccttaagc tacagttcaa ccaccgttct gctctttccc
      361 gccccagtcc ggcctgtcga tcgcggcgac cgtgatgttc tggctcaacg gcaaaccggg
      421 gtagaaaatg tcttaaagct caaagcagcc cgtcggaact tttcaccgac caatttgcac
      481 gtattcactg ctgacggaag ggtctacgcc ttcgaccttt attatactga cactctcgca
      541 tccacgtaca acctgacccg gctcgatccc ggaacgatat catccggccc ggaaattgta
      601 ctcgccggcc aggctttaaa tacggaacag cttaatgcct gcgtggatct tgttcgccgg
      661 cagcctccga aattttcaat ccgcatccat cggtttagaa tgcggctgca gcttcaagac
      721 atcgacatgg ccgggccgat attattcttt cgcttcgccg tttccaacaa gtcggatctg
      781 gactacactc cagatttcct gcggctttat ctcagtgacg aagcccgggc aaagagaacg
      841 tctcgccagg accgggagct aaccccgctc tacgccgata ccttggtaac gatccccggc
      901 agacaacgcc gcatattcat tgttggcctt ccccgcctga caatccctga ccggaaggag
      961 ttccgcctgg agctttacga aaaaaatgga ggacgaaccc tcgctcttcg tatccgcaac
     1021 cgccagctcc tacgcgccct tcgcctggcc gtccctactt ctccttcatc caatgccaac
     1081 caataatcaa cctttcgacc gcgtccttga ttccctgctt atgcaagcgg agcaagaggc
     1141 aattcaacgc acccgcaatc caactttacg ccgggatgac ccgtatttac tcggcctccg
     1201 ccaagctcaa tacgaatcta tcattgacgg ctatagaaat cgggatgacc tcacgcggga
     1261 tgaaaaacgc ggtctcacct atgtccgcac tcagctccgg aggataaatg cccgcttaca
     1321 cccaaatttg ctcagccgca ttcgctacct tcgccctgtc aactgggtat ttaattggat
     1381 acgggggcgc gcagctctga tagagggata caataatcag ctccaggacc aggaccggcg
     1441 aacgatacag gagcaaaacg tccagcgtct aagcgaggaa atgaaaaagg ccggtttcaa
     1501 agtaatcccc gaggcagctt tgaaaagggc tattgcccag gacaggccct atttcaatat
     1561 cccatatgcg gcttccgacg atgccaagac cgactacgtc ttgcatttta agaaacttcc
     1621 cggcacggat gcctattatc ttgacaattt tgacgctgct acgaagccca ctccccaaga
     1681 agtcctaaga ggaaatccca tcgtccgaca aaacttttcg ctcattgatg attattcatt
     1741 atctgcccac gaagcagcga gtgttattcg acaaataccc gtcgccagga atatcgacgg
     1801 ccaagataga tggcttatca ctgatcccct cggcgaaagg agatgggtgg aattcgacct
     1861 gcaagcggcc ctcaataaat tacctataaa agagctgcga gaccctgctc ggatgcaagc
     1921 attagtcacg gcacttcacg ctggcagctc ccgcgaggtt accttccgta agaataacca
     1981 gacacatgaa ctgcgtgtac aggtggattc ctgggcggaa ggcctgctct tcaaggacaa
     2041 gaatgggaac ctcgtcgatg tcgaccgcat tttccagtcc aacgccaatg ttcaaaaagt
     2101 tctctcgatg gctcaagacg aggctgtcgt tcgagacctt catcccagaa gatcctaata
     2161 tcaacgtcca tgcgcaccct tccatataca accctcgccg atcatgaggc tctcctcgca
     2221 gaaaagggct ttttcaaccc agagatgggt actacccgcc tcggagattg gctcaaggaa
     2281 ttccgggaaa aattcgaa
//
LOCUS       DATGKE010000658         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_353664, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000658 DATGKE010000000
VERSION     DATGKE010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353664"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VKU83_11135"
     CDS             <1..1155
                     /locus_tag="VKU83_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI94160.1"
                     /translation="IAKGPTVTDDPAGRDIVVGEAEFLRAWAYFELVTMWGPVPLYKT
                     PVNTVTGYLGQSPVADVYALIISDLTDAAAKLPNIHSTDGRATMDAANFLLGKVEMQK
                     GDYASAKTALLKLVGKYTLTSNFLDNFDGDVKLASATLATGHEFNSESIFEVVFVDKG
                     DDNFNWGYNGEGTNADGSIMRSQEYGIVWGNVIPSNAILNEFEPGDPRYKFTFYEVGD
                     KILTFAGTQPGKALTDAGMNVATSNHNGVVEKRVYRKYSVLDWTDDGFHPDGINQRIM
                     RYDEVLLMLAECEAEVGTAAQAAVYINQVRSRPSVNMPPVTLTGHDDAMKAVMHERAV
                     ELAGEEVNNIDMLRWRAKGYYPSLRPDPKPGQVALFPIPASETSANPLIK"
     gene            1225..>2493
                     /locus_tag="VKU83_11140"
     CDS             1225..>2493
                     /locus_tag="VKU83_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLI94161.1"
                     /translation="MTIRTCSYDRLWLLFSAAIFLSACAHKREDKLFTRLPADASGIH
                     FRNDLNDDDSSVSFINEFGYMGGGVGIGDFNNDGLKDILFTGNQVSCRLYLNKGNNQF
                     EDITQKAGLTTNVWATGVSIVDINNDGYDDIYICVLGKDLLHPAKNLLFINQHDLTFK
                     EEAEDYGLAFSGFSTQAVFLDYDHDGDLDMYLVNYLLGTHTNAIVPRNRSGGSPANDR
                     LFRNEGYSKEKGHPVFSDVTLQAGIREDGYGLGVTVTDFNNDGWPDIYVANDFLSNDD
                     LWLNNRNGTFTNVIGRSLQHQSYSSMGADAADVDNNALPDIITLDMLPEYNRRRKESF
                     SFMNYDRYQAERQMGYEPEFMRNMLQLNNGCLHAGDTDIPFFSEIGQLAGIQATDWSW
                     SVLIADFNNDGWKDIHITNGIGRDFINADFL"
BASE COUNT          611 a          704 c          640 g          538 t
ORIGIN      
        1 atcgcgaagg ggcccactgt cacggacgat cccgcaggaa gggatatcgt tgtcggcgag
       61 gccgaattcc tgagggcatg ggcttatttc gaactggtca cgatgtgggg tcctgtaccg
      121 ctgtacaaaa cgcccgtcaa tacggtcacc ggatacctgg gccagtcgcc ggttgccgat
      181 gtatatgcat tgatcatcag tgatcttacc gacgcggcgg cgaaactgcc caatatccat
      241 tccaccgatg gccgcgccac tatggacgcc gccaatttcc tcctgggcaa ggtagaaatg
      301 cagaaaggcg attacgcatc cgctaaaacc gcgttgctca aactggttgg caaatatacc
      361 ctgaccagca attttctcga taacttcgac ggagatgtaa aattggcgtc tgccacactg
      421 gctaccgggc atgaattcaa ttccgaatcc atttttgagg tcgtcttcgt agataaaggc
      481 gacgacaatt ttaactgggg ctataacggc gaaggtacga atgcggacgg cagcatcatg
      541 cgtagccagg aatatggcat cgtctggggc aatgtcatcc cttcgaatgc gatcctgaat
      601 gagttcgagc ccggcgaccc aaggtacaaa ttcaccttct acgaggtcgg tgacaagatc
      661 cttacgttcg ccggtacgca gcccggaaag gcgctgacgg acgccggtat gaacgtggcc
      721 acaagcaatc ataacggcgt ggtcgaaaaa agggtatacc gcaagtattc cgtcctcgac
      781 tggaccgacg atggtttcca tccggacggc attaaccagc gtatcatgcg ctacgacgaa
      841 gtgctgctca tgctggctga gtgtgaggcc gaagtcggta ccgccgccca ggccgccgtc
      901 tacatcaacc aggtgcgcag ccgccccagc gtaaatatgc cacccgtgac cttgaccggt
      961 catgacgatg cgatgaaggc agtgatgcac gagcgggcag tggaactggc cggtgaagag
     1021 gtcaataaca tcgatatgct gagatggaga gcaaaaggat attatccgtc tctcagaccg
     1081 gatcccaagc cgggacaggt ggccctgttc cccatcccgg ccagcgaaac gtcggcaaac
     1141 cctctaataa aatagacctt atcggttgtg taaaaaagag gctaatcact agcctctttt
     1201 ttttctgtac cttttcttgt cactatgacc atacgtactt gttcatatga tcggctatgg
     1261 ctgctctttt ccgcagcgat ctttctttcc gcgtgtgccc acaaacggga ggacaaactc
     1321 tttacccggc ttccggcgga cgcatccggc atccatttcc gcaacgacct gaacgacgac
     1381 gactcctccg tatcttttat caacgagttc ggctacatgg gcggaggcgt gggcatcggg
     1441 gattttaata acgacggcct taaggatatc ctctttaccg gtaaccaggt gagctgccgc
     1501 ctctacctca ataaaggcaa taaccagttc gaggatatca cccaaaaagc aggattgacg
     1561 accaatgtct gggcaaccgg cgttagcatc gtcgacatca ataacgatgg ctacgatgac
     1621 atctatatct gtgtgctcgg caaggacctc ctgcaccccg caaaaaacct gctattcatc
     1681 aaccaacacg atctcacctt taaggaggaa gccgaagact acggattggc attctccggc
     1741 ttttccaccc aggcggtgtt tctcgactac gaccacgacg gagacctcga tatgtacctc
     1801 gttaattact tattgggtac ccataccaat gcgatcgtcc cccggaaccg cagcggcggt
     1861 tcgccggcca acgaccgcct gttcaggaat gaaggctatt caaaagaaaa aggccatccc
     1921 gttttctccg acgtcaccct tcaggcaggg atccgcgagg acgggtacgg actgggcgtt
     1981 acggtcaccg actttaacaa cgacggctgg cccgatatct acgtcgccaa tgattttctt
     2041 tccaatgacg acctgtggct caataaccgc aacggcacct ttaccaatgt tatcggcagg
     2101 tccctgcagc accaaagcta ttccagcatg ggcgcagatg ccgcggatgt ggacaacaat
     2161 gccctcccgg atatcatcac cctcgatatg cttcccgaat acaaccggcg gcggaaagaa
     2221 tccttctcgt ttatgaatta tgaccgctac caggcggaac gccagatggg atacgaaccc
     2281 gagttcatgc gcaatatgct ccagctgaat aacggctgcc tgcacgccgg cgatacggac
     2341 attcctttct tcagcgagat cggccagttg gctggcatcc aggcgacaga ctggagctgg
     2401 agcgtgctga tcgcggattt taacaacgac ggctggaaag acatccatat caccaacggc
     2461 atcggcaggg attttatcaa cgccgacttc ctc
//
LOCUS       DATGKE010000659         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1014250, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000659 DATGKE010000000
VERSION     DATGKE010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014250"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VKU83_11145"
     CDS             complement(<1..701)
                     /locus_tag="VKU83_11145"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLI94162.1"
                     /translation="MKKMLLFFSLSLFAVAGFTQTPNTLTQKEKDEGWILLFDGSTTN
                     GWHTYLRDTAGSRWQVRNGALVFDPTKPENGGGDLVTNGVYENYELNLEWKISKGGNS
                     GIIFDVQEDPKYPATYITGPEMQVLDNISADDNKKQNHLAGCLYDMAGDSSVSKPNPV
                     GEWNHVRLIQNHGHLIFWLNNIKTFEGTIGSDEWKRMIARSKFRNKDFADFAKVAKGH
                     IALQQHPGSSEWRNI"
     gene            803..2680
                     /locus_tag="VKU83_11150"
     CDS             803..2680
                     /locus_tag="VKU83_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLI94163.1"
                     /translation="MNMLVFFSRRIWLAVLFLSPYVVVSAAGGRIHIVPEPVSVVERP
                     GSFILRQGATLAVEGDSLSGISAWFVAQVKEQTGISLREEKAGRGAIFVHVGKDKEAG
                     REGYSLTVSSSGIHINASAPAGAFYGMQTFLQLLPPGRADFMSVPCVEIADSPRFAWR
                     GLMLDVSRHFFTKQEVMKYIDEMVRYKYNTLHLHLSDDQGWRIEIKSLPQLTEHGAWR
                     VQRTGRWGEFLPPLSDEPVTDGGFYTQDDMREIIRYAQERFVTILPEIDVPAHSLALI
                     SSYPNLSCTQLPYPVNPGARPPVRQDNALCIGNDSVYLVLDKIFTELAALFPCPYIHV
                     GGDEAYKGFWATCPKCQKRMADEHLKNVEELQSYFVKRMEKMLTAKGKKLIGWDEILE
                     GGLAPSATVMSWRGMAGGIAAARMGHQVVMTPWDYCYLDLYQGEPTVEPPTYGLCRLK
                     DSYTYDPVPDSVDERLILGGQGNLWTESVPNFRQVEYMTWPRGLALAEVYWSPKSKRN
                     WDDFIGRMEAEFQRMDAANIKYARSAYNVIFTPKRSDDGTMAVGMSTEIDGLDIYYTF
                     DDTNPDKFYPRYDGTPVKFPLGANQLNVITYRNGHPIGQQVNIRKDDLMKRLDQGRHV
                     Y"
     gene            2794..3342
                     /locus_tag="VKU83_11155"
     CDS             2794..3342
                     /locus_tag="VKU83_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HLI94164.1"
                     /translation="MADLIVYQSKYGATAEYAQWVGEALRTDAVPVGSLKPMQLVHAD
                     RIIIGTPVYLGKMLMRHWIAQHQGYLRNKPVYLFVVCGTPAGDSEKTGKIVKDNIPAP
                     IRDKFRIYFLPGRVMMDKLSWMDRFLIRLAARAKKKASGETLATGVNYVKKENLAALL
                     GTLDRSPVGPSRRSEAGKGFLL"
BASE COUNT          804 a          867 c          982 g          823 t
ORIGIN      
        1 ttgatatttc gccattcgct ggaaccggga tgctgttgca gggcgatgtg tccctttgcg
       61 acttttgcga aatcagcgaa atccttattt cggaatttgc tccgggcaat catccttttc
      121 cattcatcgc tgccgatcgt gccctcaaaa gtcttgatat tgttcaacca aaaaataagg
      181 tgcccgtgat tttggatgag ccggacgtgg ttccactctc cgacggggtt cggcttgctg
      241 actgaagaat ctcccgccat gtcatagagg caaccggcca ggtgattctg cttcttattg
      301 tcatcagcac taatattatc caggacctgc atttccgggc cggtaatata ggtagcaggg
      361 tatttggggt cttcctgcac gtcgaagatg atgccgctgt taccgccttt cgagatcttc
      421 cattcgaggt tcagctcata gttttcataa acaccgttcg tcaccaggtc gccgccgcca
      481 ttttcgggct ttgtaggatc gaaaaccagc gctccattcc tcacctgcca cctggatccg
      541 gcggtatcgc gcaggtaggt atgccatccg tttgtcgtgc tgccgtcgaa gaggaggatc
      601 cacccctcgt ctttctcctt ttgcgtcagc gtattggggg tttgggtgaa acccgcgacg
      661 gcaaagagag acagcgagaa gaagagcagc atttttttca tgacgtggag tttttgatcc
      721 gacaatttag gaaatatcgg caataaaact tttgctaata aagctaaacg ggtaaattca
      781 tgcattcaat tccaaaacgt ttatgaacat gcttgtcttc ttttcgcgcc gtatctggct
      841 ggctgtacta ttcctgtctc cttatgttgt tgtttcggct gctggtggcc gtatccacat
      901 tgtccccgaa ccggtttccg tcgttgaaag gcccggaagc ttcattttgc ggcaaggtgc
      961 aacgctggca gtagaaggcg attccctttc ggggatcagt gcctggttcg ttgcgcaagt
     1021 gaaagagcag acggggatct cgctgcgcga agaaaaagcc ggccggggag caatctttgt
     1081 ccatgtcgga aaagacaagg aagcgggcag ggagggatat agcctgacgg tcagctcatc
     1141 gggcatccat ataaatgcca gcgctccggc gggcgccttt tacgggatgc agaccttttt
     1201 acagttgctg ccgcccggcc gggcagattt catgtctgtc ccctgcgtgg agatcgccga
     1261 ctcgccgaga tttgcgtggc gtggcctgat gctggatgtc agccgccact tttttaccaa
     1321 gcaggaagtg atgaagtata tcgatgaaat ggtgcggtat aaatataaca cgttacatct
     1381 gcacctcagc gatgaccagg gctggcgcat cgaaataaag agcttaccgc aactgaccga
     1441 acatggtgca tggcgggtgc agcgaacggg gcgttggggc gagttcctgc ccccgttgag
     1501 tgatgagccg gttacggatg ggggattcta cactcaggac gacatgcgcg agatcatcag
     1561 gtacgcccag gaacgcttcg tgaccattct gcccgaaatc gacgttcccg cgcatagcct
     1621 cgcgttgata tcttcttatc ccaatctatc gtgtacgcag ctgccatatc ccgtcaatcc
     1681 cggcgcccgc ccgcctgtca ggcaggacaa tgcactttgt attggcaacg actcggtcta
     1741 cctggtgctg gacaagatct ttaccgagct ggcagcgctc ttcccctgtc cttacatcca
     1801 tgtcggcggt gatgaagcgt ataagggatt ctgggccacc tgcccgaaat gtcagaagcg
     1861 aatggctgac gagcacctca aaaatgtcga ggaacttcaa agctattttg tcaagcggat
     1921 ggagaaaatg cttacggcaa agggaaaaaa gctgattggc tgggatgaga tcctggaagg
     1981 gggccttgcg cctagcgcga cggtaatgag ctggagggga atggccggcg ggatcgctgc
     2041 cgcaaggatg ggacaccagg tcgtgatgac cccttgggat tattgctacc tggatctcta
     2101 tcagggcgaa ccgaccgtag agccgcccac ctacggctta tgcaggttga aagactcata
     2161 cacctatgac cccgttcccg acagcgttga tgagcgcctg atcctgggcg ggcaagggaa
     2221 tctttggacc gaaagcgtgc cgaatttccg gcaggtggaa tatatgacct ggccgcgcgg
     2281 gctggcgttg gcagaagtct actggagccc aaaatcaaag cggaactggg acgatttcat
     2341 cggcaggatg gaagcagaat tccagcggat ggacgcggcg aatatcaaat acgcccgaag
     2401 cgcgtacaat gtcattttta cgcctaagag gtcggatgac ggaacgatgg ccgttggaat
     2461 gtcgaccgaa atcgacggac tggatattta ttatactttc gatgatacga acccggacaa
     2521 gttctacccc aggtatgacg gcacgccggt aaaatttccg cttggcgcca atcagctgaa
     2581 tgtgattacc taccggaacg ggcatccgat cgggcagcaa gtgaatatca ggaaggacga
     2641 tctgatgaaa cgcctggatc aagggcgcca tgtttattaa ctgctacggc tagggccagt
     2701 cgccggcgtg acgagaatca tgtccgggcc gggcagggat catcaccggt cgcgaaagga
     2761 gggcataatt tcacccggta aaacttttgc gacatggcag acctgattgt ctatcaatcc
     2821 aaatatggtg ctaccgcgga atatgcgcaa tgggtgggtg aggcattgag aaccgatgcg
     2881 gtcccggtag gatccttaaa gcccatgcaa cttgtccatg cagaccgcat catcatcggg
     2941 acgccggtgt atctgggaaa aatgctgatg cgccattgga tcgctcagca ccaaggctat
     3001 ctgcggaata agccagtcta tctttttgtt gtttgcggga ccccggccgg cgacagcgag
     3061 aaaacgggca agatcgtaaa ggataatatt ccggcaccca ttcgggacaa gttcaggatc
     3121 tactttcttc cggggcgggt tatgatggac aaactatcgt ggatggaccg gttcctgatc
     3181 cggctggcgg cccgggcgaa gaaaaaagct tccggcgaaa cgttagcaac gggcgtgaac
     3241 tacgttaaaa aggaaaacct tgccgctttg cttggtacct tggaccggag cccggttggt
     3301 ccgtcaaggc ggtcggaagc gggaaagggt tttttactct aaggcatcat taagaatgtt
     3361 tagcctgagg caagaggcga aaccgttatt tttgcggaaa taacgggcaa tatgcgcaac
     3421 caactctttc gctgtattat ttttctgttt cttatttcta tccggaccgg cgtcaa
//
LOCUS       DATGKE010000660         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1180086, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000660 DATGKE010000000
VERSION     DATGKE010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180086"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VKU83_11160"
     CDS             complement(<1..664)
                     /locus_tag="VKU83_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94165.1"
                     /translation="MMEWLGTKPHDEYRQGEAYAPAVADGRDFGKKFYIESYGCQMNF
                     SDSEVVASILKDNGFGATRNYEEADLVLLNTCSIREKAEQTVRNRLQLFRQVKDARPG
                     MLVGVLGCMAERLKGKFLEEEQLVDLVVGPDAYRSLPGLIGEAGAGQKAVNVLLSRDE
                     TYADISPVRLDSNGVTAFVSIMRGCNNMCSFCVVPFTRGRERSRDWASIVRECRDLFD
                     RGF"
     gene            complement(820..892)
                     /locus_tag="VKU83_11165"
     tRNA            complement(820..892)
                     /locus_tag="VKU83_11165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(857..859),aa:Gly,seq:ccc)
     gene            complement(957..1484)
                     /locus_tag="VKU83_11170"
     CDS             complement(957..1484)
                     /locus_tag="VKU83_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94166.1"
                     /translation="MITTENVSLPRDFAADSRVWIYQANRPFTGAEADEARGLLDSFI
                     AEWDSHGHPVKGFAGMFYNQFVVLVADETATGVSGCSTDSSVRLIRQLEDRTGVRLFD
                     RLNLAFLVEGRVQLIPMTQLSSALEAGTITGETAYFNNTVRTKQEFETDWLIPLKQSW
                     LGSKHLHDCKAAPAK"
     gene            complement(1494..2273)
                     /locus_tag="VKU83_11175"
     CDS             complement(1494..2273)
                     /locus_tag="VKU83_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLI94167.1"
                     /translation="MEVPTVAELIAKGESPEILFWVGCSGSLDQRAQKITRAFASILT
                     KVGISYAILGKEEACTGDPARRAGNEFLFQMMAWQNIQTLNNYGIKKIVTACPHCFNI
                     LKNEYPELGGHYEVIHHTMLLQQLIDEGKVRLKEGGPFKGKRITYHDSCYLGRANGVY
                     EAPRQVLAALDAELIEMKRCRSKGLCCGAGGAQMFKEEEKGTTRVNYERSEEALGTGA
                     AIIASACPFCNTMLTDGVKHEEKEGDVRVMDIAELIAESID"
     gene            2368..3069
                     /locus_tag="VKU83_11180"
     CDS             2368..3069
                     /locus_tag="VKU83_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLI94168.1"
                     /translation="MAGDESMLLIRNYDLWCNLSAEEYKDLRVEHHVMDVPKGEYIYF
                     EAYNHNKIYFVKEGTIKIGYIDQEGNEKVKEIIQQGELFGQFSLERNNLHGEFAQAYR
                     NDVSLCAFTIDDFERLLKNRPDLALRYSKQVGAKLRNIENRLINLLNKDVKTRLIHFL
                     WQLVEQNLGENTAEGFCIPNYLTHEDIASLIGSSRQTVTTMINELEAEGILSYNRQEI
                     CFMNVKKLQKLVDEN"
     gene            3077..3580
                     /locus_tag="VKU83_11185"
     CDS             3077..3580
                     /locus_tag="VKU83_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLI94169.1"
                     /translation="MRYLLFSCFLQLAIGSLSAQRAGHAFTPADEGSTIDFTIKNLGF
                     DSKGTFKGIQGTIVFDPANPAGDSFDVSIDASSVNTDNNMRDDHLRADTYFDVARYPR
                     ISFVSTSVSAVDKSGHYTVTGNLTIKGVTRPISFPFLATPAGNDYIFNGSFAINRRDL
                     AWGERAR"
     gene            complement(3687..>3940)
                     /locus_tag="VKU83_11190"
     CDS             complement(3687..>3940)
                     /locus_tag="VKU83_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLI94170.1"
                     /translation="DDGKNLLNYISVEELRACTTCNACVEECPVSISPLEIIMEMRRA
                     LIMEDSNAPQEWSAMFSNVENNFAPWKYSPDDRDKWVEG"
BASE COUNT          894 a         1039 c         1068 g          939 t
ORIGIN      
        1 tgaaaccccg gtcgaacagg tcgcggcatt cccggacgat ggaggcccaa tcccggctgc
       61 gttcccggcc acgggtaaac ggcacgacgc agaacgagca catattattg cagccccgca
      121 taatgctgac aaacgcggta acgccgttgc tatccaggcg gaccggagaa atatccgcat
      181 aagtctcgtc ccggctcagc agcacattga ccgccttctg gccggcccct gcttcaccga
      241 tcaggccggg taagctgcgg taggcgtcgg gaccgacgac gaggtcgacc agttgctcct
      301 cttccaggaa tttgcctttc aaccgctcgg ccatgcatcc gagcacccca accagcattc
      361 ccggccgggc gtctttgacc tggcgaaaga gctggaggcg gttccgtacg gtttgttcgg
      421 ccttttcgcg gatgctgcag gtattcagga gcacgagatc ggcttcctca tagttgcgtg
      481 ttgcgccaaa accgttatcc ttcaatatgg atgcgacgac ctcgctgtcc gaaaaattca
      541 tctggcaacc atagctttct atatagaatt tcttcccgaa gtcccggcca tcggcgactg
      601 ccggcgcata ggcttcgccc tgcctgtatt cgtcatgagg ttttgtcccc aaccattcca
      661 tcatccttgc ccttttttaa agtgcaaaga taagcagaaa acgcatagga tgtgacaact
      721 tggcagagaa gggttgccgg agggcaagtt ttccgggata gtcctgaaat gcaagcccgg
      781 aaaatagaaa agcctcagtt gaacctgaga ctttatggga gcggaagacg aggatcgaac
      841 ccgcgacctg cagcttggga agctgccgct ctaccaactg agctacttcc gcggaatttc
      901 ggcgattaaa ccggtgtgca aatgtaaggg tgaaaccctg atgcctaaaa atatttttat
      961 ttcgccgggg ccgctttgca atcatgaaga tgtttagatc ctaaccagct ttgtttcaag
     1021 ggaatcagcc agtccgtctc gaattcctgt ttcgtccgga ccgtgttatt aaaataggcg
     1081 gtttcacccg ttatggtgcc ggcctcaagc gcgctggaaa gttgggtcat cggaatcagc
     1141 tgaacccggc cctccacgag gaaagcaagg tttaggcggt cgaagaggcg gacaccggtc
     1201 cggtcttcga gctgccggat aagccgtacg gagctgtcgg tgctgcaccc actgactcct
     1261 gtagccgttt catctgccac cagaacaacg aactggttat agaacattcc cgcgaagcct
     1321 tttacaggat ggccatgaga atcccattcg gcgataaagg agtcgagcag tcctcttgct
     1381 tcgtcggctt cggccccggt aaatggtcgg ttggcctgat agatccagac ccgggaatct
     1441 gccgcaaagt cgcgggggag cgatacattc tctgtagtaa tcatcttgca aagttagtca
     1501 atcgattcgg caatcagctc ggcaatatcc atcaccctga catcgccttc cttttcttcg
     1561 tgttttacgc cgtctgtcaa catggtatta caaaaagggc aggccgaggc gattatcgcc
     1621 gcgccggttc cgagggcttc ttcgctgcgc tcgtaattga ctcgtgtcgt tcctttttcc
     1681 tcttccttga acatttgagc gccgccagcg ccgcaacaca gacctttgct tcggcaacgt
     1741 ttcatttcga taagttccgc gtccagtgcg gcgagtacct gcctgggggc ttcatagacg
     1801 ccgttggcgc ggccgaggta acaactgtcg tggtaagtaa tccgctttcc tttgaacggg
     1861 cctccttctt tgagcctcac tttgccttcg tcgatcaact gctggagcaa catggtatga
     1921 tggatgacct cataatggcc gccaagttcc ggatactcgt tctttaaaat gttgaaacaa
     1981 tgcgggcagg ccgtgacgat cttctttatc ccgtagttgt tgagcgtctg gatattttgc
     2041 cacgccatca tctggaaaag gaattcgtta cccgcgcgcc gggccgggtc gcccgtacaa
     2101 gcctcttctt tccccaggat ggcatagctg atcccgacct ttgtaagtat gctggcaaat
     2161 gcccgggtga tcttttgggc ccgctgatcg agactgccgg agcaaccgac ccagaacaaa
     2221 atctcagggc tttctccttt tgcgatgagt tccgcaacgg tgggcacttc catgtggaat
     2281 atttttccgt aaatctaacg gaaaggcgcc aaatgcatgt tttcgagccg gaaatgttat
     2341 ctttagccgc aaaattggta attcgttatg gcgggtgacg agagcatgct gttgatccgt
     2401 aattatgact tatggtgcaa cctttcggcc gaggaatata aggacctgcg ggtcgaacat
     2461 cacgtgatgg atgttccgaa aggagagtac atttatttcg aggcgtataa tcataacaag
     2521 atctattttg ttaaagaggg gacgatcaag atcgggtata tcgaccagga aggaaacgaa
     2581 aaagtgaagg aaatcattca gcagggcgag ttgttcggtc aattcagcct ggagcggaat
     2641 aaccttcacg gcgaatttgc ccaggcttat cgcaacgacg tcagcctctg cgcttttacg
     2701 atcgacgatt tcgagcggtt gctcaagaac aggcccgacc tcgccctgcg ttacagcaag
     2761 caggtgggcg ccaagttgcg caatattgag aaccggctga tcaatctcct gaacaaggac
     2821 gttaagacgc ggctgatcca tttcctgtgg cagctcgtgg agcagaatct cggcgaaaac
     2881 actgccgaag gtttttgtat acctaattac ctaacacatg aggatattgc cagtctgatc
     2941 ggcagcagcc ggcagaccgt tacgaccatg atcaacgagc tcgaggcgga agggatacta
     3001 tcctacaacc gccaggagat ctgttttatg aacgtaaaga aacttcaaaa actggtggac
     3061 gaaaattaga atggccatgc gatatctcct cttctcctgt ttcttacaac tggcgatcgg
     3121 ttcattatcg gcacagcgtg ccggacatgc ctttactccc gcggatgaag gctcgacgat
     3181 tgattttacg atcaagaacc tgggtttcga tagcaaaggg acgtttaaag gcattcaggg
     3241 aacgattgtt tttgatccgg caaatccggc cggtgacagc tttgacgtca gcatcgacgc
     3301 ctcttcggtg aatacggata ataatatgcg ggacgatcat ctgcgggcgg atacgtactt
     3361 cgatgtggcc aggtacccga ggataagctt tgtctcgacc tcggtctccg ctgtggacaa
     3421 gagcgggcac tataccgtga ccggcaatct gaccattaag ggagtgacca ggccgatcag
     3481 ttttcccttt ctcgcgacgc cggcggggaa cgactatatt ttcaatggta gctttgcgat
     3541 caaccggcgc gacttggcgt ggggggagcg agcacgctga gcaaccaggt gaccgtgtca
     3601 ctgaccgtgc tggcaaggag ggaataattg cggacccccc cgggacgggt gcgcgcgccg
     3661 ggagggctta ggcgagggca tgggtgtcat ccttccaccc atttatcccg gtcgtccgga
     3721 ctgtatttcc agggggcgaa attattttca acgttgctga acatggcgct ccattcctgg
     3781 ggagcgttgc tgtcctccat gatcagcgcg cggcgcatct ccatgataat ttccagcggg
     3841 ctgatgctta cagggcattc ttcgacgcaa gcgttgcagg tggtgcaggc gcgcagctct
     3901 tcaacggaaa tgtaattcag cagattcttg ccgtcatcgg
//
LOCUS       DATGKE010000661         5235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_849909, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000661 DATGKE010000000
VERSION     DATGKE010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849909"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VKU83_11195"
     CDS             complement(<1..1610)
                     /locus_tag="VKU83_11195"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94171.1"
                     /translation="MSKLLILRRVIFSGGLAAAGCLFCSRACSQDSGLFNDLSWRMIG
                     PHRGGRTVGGCGVPQEPNVFYIGVNNGGVWKTTDFGRTWDPIFDQEPTGSIGDVAVAP
                     SNPDIVYVASGEGIQRPDLSVGDGVYRSSDAGKTWVHLGLAEGQQMGGLAVDPKDPDR
                     VFVAVLGHPYGPNPERGVYRSTDGGKNWEKVLYKDENTGAVQVTIDPVNPQVVYADLW
                     AGRQGPWENGAWNGPNSGLYKSTDGGTTWRQLKQGLPDVGQGLSRIGFCIAPSDPSRL
                     YATVAADPTYGGIYRSDDAGETWKKLNSDERFWGRGDDFAEVKADPKNKDIVYTADVV
                     VWKSNDGGMSWNAFKGAPGGDDYHRMWINPDHPDIILIACDQGAIVTVNGGRTFSSWY
                     NQPTAQFYHVSTDNAFPYNVYGGQQESGSIGIASRGNDGEITFREWHPVGVEEYGYVA
                     PDPLDPNIIYGGKITRYDKRTGQVQNIAPEAIASGKYRFLRTAPVLFSPTDPHVLYFA
                     GNVLFKTRDGGHSWQVISPDLSRETWSVP"
     gene            complement(1673..2938)
                     /locus_tag="VKU83_11200"
     CDS             complement(1673..2938)
                     /locus_tag="VKU83_11200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLI94172.1"
                     /translation="MTMISGNSERLVQAVASNHRELFRQEAIALGGRMIESRGLSWTS
                     GTPHSSSMIAFPEMDAESAGQQLDEVIAFYLREPPGGAACWALDPPVPGDLGIRLLAR
                     GFQPGWRPHWMAADLTQLSSHHPEPRNLRIVPDNETSLLAVPDLPYARVVIPDPATAE
                     FPGQWTRFVAWVRGKVVGQSVVFLTSGPLGVAGIYHVGVVPGSRSKGIGKAVTRAACL
                     YAQERGYRYAVLNSTEAGKRPYGQLGFWSTGDGWTWWLTTERLLAQPPEPAQVRLAEA
                     IGRGDEAGLSALASGTGKAEVVTMLTNGMSLLQLAVHCRQPQAAEWLIARGAEYSALD
                     AWDLGWKDRAGELLRSDREQIHRLYGKEGKNLLHIAVERNDEALAELALAAAPDLKWR
                     DNTWNSTPLEWANHFGYSNIARMIRERKS"
     gene            complement(3065..4108)
                     /locus_tag="VKU83_11205"
     CDS             complement(3065..4108)
                     /locus_tag="VKU83_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLI94173.1"
                     /translation="MRLFTNLLFKVTVSSMILTAPAMIDGRTNAGGMWLDKKTDMPFA
                     SANVIKNYIDHMTVWPVHAYVDKQSMVAVFGAPTTALAGPTAAAKAIGAAAAEATGTA
                     ATPATAVTANAVKEVVRTAERIATPAEMKLGATSASFARAAALRRVIRESNILYTAIG
                     LEKEGLSEEAFEYAWRGYHNLLKRGSIRRKSVLSIADFSQSSSEKRMYVIDIRHRRLL
                     FRTYVAHGQNSGAEFAESFSNEPESFKSSLGFYVTKKTYVGRNGLSLRLNGVDPGFND
                     KAMKRNIVLHGCSYVGDRYLESFGETGTSLGCPALPSSISRPIIQKVKNGSCFFIYHP
                     TEDYLDHSVVINE"
     gene            complement(4199..4531)
                     /locus_tag="VKU83_11210"
     CDS             complement(4199..4531)
                     /locus_tag="VKU83_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94174.1"
                     /translation="MAPLAVPFFIKNDLHMPDFAIPIDIYVLAALLTLAVLAGFGLGK
                     QLLAKQKRKTAELEREMMQAHAELLETQRGYCELESKVKEEGSLVIPIKKKIEPGSAP
                     SAGKSENF"
     gene            4821..>5235
                     /locus_tag="VKU83_11215"
     CDS             4821..>5235
                     /locus_tag="VKU83_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94175.1"
                     /translation="MINNFINLFLLKRQVRVKQKALAQTVNQLKETVSRLEASEQALL
                     KTNAQREKLISLVIHDLRSPLRFLTYLAGDLYDNHANLSPAELRERAYLLKKGTQDIY
                     HFSADFLLWVTSQKDKFSINRRNFPIGPLLQEIVDF"
BASE COUNT         1124 a         1586 c         1365 g         1160 t
ORIGIN      
        1 ggcacgctcc aagtctcccg gctgaggtcc gggctgatca cctgccagga atgacctccg
       61 tcgcgcgtct tgaaaagcac attcccggca aaatacaaga cgtgcgggtc cgtgggcgag
      121 aacaacaccg gcgctgtccg caggaaacgg tacttaccac tggcaatggc ctccggtgcg
      181 atattctgca cctgtccggt ccgcttgtca tagcgggtga tctttccgcc ataaatgatg
      241 ttggggtcga gcgggtcggg cgcgacgtac ccatactcct cgactcctac cggatgccac
      301 tcgcggaacg tgatctcgcc gtcatttcca cgggaagcga tcccgatcga gccgctctcc
      361 tgctggccgc cgtacacatt gtagggaaaa gcgttatcgg tacttacgtg gtagaactgg
      421 gccgtcggct gattatacca ggaagaaaaa gtccggccgc cattgacagt tacgatggcg
      481 ccctggtcac aggcgatcag gatgatatcg ggatgatccg ggttgatcca catccggtga
      541 tagtcgtcgc cgcccggcgc ccccttgaag gcattccagg acatcccccc gtcgttggac
      601 ttccagacca ccacgtcggc ggtgtacacg atgtccttgt ttttgggatc ggccttcacc
      661 tcggcgaaat cgtcgcccct gccccagaac cgttcgtcgc tgtttaattt tttccaggtc
      721 tcccccgcat cgtcgctgcg gtagatgccg ccataggttg gatcggccgc cacggttgca
      781 tacaggcgtg acggatcgct cggcgcaata caaaaaccta tccgcgatag tccctggccg
      841 acatccggca aaccctgctt cagctggcgc caggtcgtgc ccccgtccgt ggatttgtat
      901 aaaccgctgt tcggaccgtt ccaggcgccg ttctcccatg gaccttgtcg tcccgcccag
      961 agatcggcat ataccacctg aggattgaca ggatcgatgg ttacctgcac tgcaccggta
     1021 ttttcgtctt tataaaggac cttttcccag tttttgcccc cgtcggtgct ccgatagacc
     1081 ccgcgttccg gattgggtcc gtacggatgg ccgagaaccg ccacaaaaac cctgtccgga
     1141 tccttagggt ccaccgccag tccgcccatc tgctggcctt cggccagccc gaggtgcacc
     1201 catgttttcc cggcgtccga ggaccggtaa acgccgtcgc ccacggaaag gtcgggccgt
     1261 tggatacctt cgccgctcgc cacatacacg atatccggat tggaaggcgc caccgctacg
     1321 tcgccgatcg acccggtcgg ctcctgatcg aaaatgggat cccaggtgcg gccaaaatcg
     1381 gtcgtcttcc aaacgccgcc gttattcacg ccgatgtaaa acacgttcgg ctcctgcgga
     1441 accccgcagc cgccgactgt tcgcccgccg cggtgcggac cgatcatgcg ccagctaaga
     1501 tcattaaata gcccggaatc ctgcgaacag gcccgggaac agaataggca tccggccgcc
     1561 gcaagccccc cggaaaagat cacccggcgg agaataagca gtttgctcat gtgatggtag
     1621 ttatagaagg gttggaaagt acgacatatt gctgcacgcc gcctaccgcc catcaacttt
     1681 tcctttcccg tatcatccgc gcaatgttcg aataaccaaa atggttagcc cattcgagcg
     1741 gggtcgaatt ccaggtgttg tcccgccatt tgaggtccgg cgcggccgcg agcgccagtt
     1801 ccgccagtgc ttcgtcattg cgctccacgg cgatatgcag caggttcttt ccctcttttc
     1861 cgtatagccg gtggatttgt tcgcggtcag atcgcagcag ttcccccgcc ctgtccttcc
     1921 atccaaggtc ccaggcgtcc agcgcgctgt attcggcgcc ccttgcgatc agccattccg
     1981 cggcctgggg ttgcctgcaa tgcaccgcca gctgcagcag gctcatccca ttggtgagca
     2041 tcgtaacgac ttcagccttg cccgtcccgg aggcgagggc actcagcccg gcttcgtcgc
     2101 cacgcccgat ggcttccgcc agcctgacct gggccggttc gggtggttgc gcaagcagcc
     2161 gttcggtcgt cagccaccat gtccaaccgt ccccggttga ccagaagcca agctgcccgt
     2221 aaggtcgttt ccccgcctct gtcgaattca ggacggcgta ccggtatccc cgctcctgtg
     2281 cataaaggca cgcggcccgc gtcacggcct tgccgatccc cttgcttctg gatccgggaa
     2341 ccacacccac atgatagatg cccgcgacgc cgagcggccc tgaggtcagg aacaccacgc
     2401 tttgaccgac cactttgccg cgtacccaag ccacaaacct ggtccactgg ccaggaaact
     2461 cagccgtagc cggatccgga atgaccaccc tggcataggg cagatccggc acggccagga
     2521 gggaagtttc attatccggg acaatccgca gattcctggg ctccggatgg tgagaagaca
     2581 gctgggtgag atccgccgcc atccagtggg gccgccaacc cggctggaat ccgcgggcaa
     2641 gcagcctgat cccgaggtca cccggaaccg gcggatccag ggcccagcaa gccgcaccgc
     2701 ccggcggttc acgaagataa aacgcgatga cctcatcgag ttgctgaccg gcgctttcgg
     2761 catccatttc cggaaaagcg atcatggagc tgctatgggg cgtgccggat gtccaggaca
     2821 ggccacggga ttcgatcatc cggccgccga gcgcgatcgc ctcttgacgg aagagctccc
     2881 ggtgattgga cgccacagct tgtacaagcc gctctgaatt cccgcttatc atggtcatcg
     2941 gcggagtcat cgctataaaa ctagtcgatc ggccgcagca ggcaaaattt atcgccgcac
     3001 cctcgctggg caaaaaaaag ggcggatcaa gcgatccacc cgttgcggct atctgatcca
     3061 agcctcattc gtttatgacc accgaatggt cgaggtaatc ttctgtcgga tgatagataa
     3121 agaaacagct gccatttttc accttttgaa taataggcct gctgatggac gagggcagcg
     3181 ccgggcaacc gaggctcgtg cccgtctcgc cgaaactctc caggtaccgg tcgcctacgt
     3241 atgagcagcc atgcagcact atgttccgct tcatcgcctt gtcattaaaa cccggatcaa
     3301 ccccgtttag ccgcagtgaa agtccgttgc ggccaacata ggtctttttt gtcacataga
     3361 aaccaaggct gcttttgaat gattcgggtt cgttggaaaa agattcagcg aactcggcgc
     3421 ccgagttctg tccatgagcg acataggttc ggaataacag ccgcctgtgc cggatgtcga
     3481 tcacatacat tcgtttttcc gaagaagatt gactgaaatc agcgattgat aatacgcttt
     3541 tccgcctgat gcttcccctt tttaataaat tgtgatagcc tctccaggca tattcgaaag
     3601 cttcttcact cagcccttct ttttcaaggc cgattgccgt ataaaggatg ttcgattcgc
     3661 ggatgacccg ccggagtgcc gcagccctgg cgaaagacgc cgacgtcgcg ccgagcttca
     3721 tttcggctgg cgtcgcgata cgctccgctg tccgaaccac ctctttcacc gcattcgcag
     3781 tcacagccgt tgcaggcgtt gcagcggtcc cggtcgcctc cgcagccgca gcgccgatcg
     3841 ccttcgccgc cgctgtcgga cccgccaatg cggtcgtggg ggccccaaag acagctacca
     3901 tcgactgctt atccacatag gcatggacgg gccacaccgt catgtgatcg atataatttt
     3961 tgatgacatt ggcggaggca aaaggcatgt ctgtcttctt atccagccac attccgcccg
     4021 cgttggtccg gccgtcgatc atcgccggag ccgttaggat catagaagat accgttacct
     4081 taaacagcag gttcgtaaag agtctcatcg gtttggattt tataatagga tatacagatt
     4141 taatggacgg ataccagggg atatcttccc ttgatataac gcgaattgac gggaaatatt
     4201 aaaaattttc gctttttccc gccgaagggg ccgatcccgg ttcgattttc tttttaatcg
     4261 ggatcaccag gctaccctct tcttttacct tggattccag ttcgcaatag ccccgctggg
     4321 tctcgagcag ctcagcatgc gcctgcatca tctcccgctc cagctcagca gtctttcgtt
     4381 tttgtttggc cagcaactgt ttgcccaggc caaaaccggc cagcaccgcc agcgttagca
     4441 aagcagccag gacatagatg tcaatgggaa tggcaaaatc cggcatatgg agatcatttt
     4501 taatgaagaa gggcaccgcc aggggcgcca tatagtcaaa atgttccgtg ccggtcaggg
     4561 aatgcctttg aaaagaatag atgcaggact tttaaggtaa atttggctca tagggtaccg
     4621 tttggtttat ataaaaatac cccttgtccc gttcccggct ttgctcataa tccgaaaaaa
     4681 tgtccgcaag cggacatcgc aatcaaataa ttgatttgta gaatattgcg catatcgatt
     4741 aaaatatatg tttaaagaat cgacaggcct ttagccgcta ttcgtttttt ttctactact
     4801 tttagcctta aggttaaggc atgataaata actttatcaa cctattttta ctaaaaaggc
     4861 aggtccgggt gaagcaaaag gcgcttgccc aaacggtcaa ccagctaaag gagacggttt
     4921 cccggctcga agcatcagaa caggccctgc tcaaaaccaa cgcgcaacgc gaaaaactga
     4981 tctcgctcgt cattcacgac ttgcgctcac cactccgttt ccttacctac ctggcggggg
     5041 acctttatga caaccatgcc aacctttccc cggccgaact cagggagcgt gcttatctct
     5101 taaaaaaggg gacacaggat atctaccatt tttcagccga ctttctgctg tgggtgacga
     5161 gccagaaaga caaattcagc ataaaccgcc gcaattttcc tatcgggccg ctcttacagg
     5221 agatagttga cttct
//
LOCUS       DATGKE010000662         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1493489, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000662 DATGKE010000000
VERSION     DATGKE010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493489"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VKU83_11220"
     CDS             <1..344
                     /locus_tag="VKU83_11220"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HLI94176.1"
                     /translation="GGASWDTAYFDSHLADPVCQGSIFAVGREKGHSIIAECNNDDSV
                     QRNNLTLRISYDEGRSWTRPQVVDRSDEASKKKDYTAYSDIVGLSAKTIGVLYERNNY
                     KEITFSILKWR"
     gene            344..1717
                     /locus_tag="VKU83_11225"
     CDS             344..1717
                     /locus_tag="VKU83_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HLI94177.1"
                     /translation="MKTIRCSLLLLAPLLLHAQTRLPAYPDSLFPTYYHQRVTHFQTL
                     HRSCTDILFLGNSITDGAEWSELFNDARIRNRGISGDITAGVLHRLAYLAGGTPEKVF
                     LLIGINDLSRGTSPDSVVKNILLIADYLHEQTPATHLYVQSLLPVNDGFGKFGTHTNK
                     GRDILVVNRSLQNDAESHHYSFVDLYSSFCDSAGKLRSAFTNDGLHLTGEGYLLWKHL
                     LYADVFDLAEKPSLIPLPWQLQWRPGYFWLSHCAGIAVNDSALIREARFLQEELSRKG
                     IRLPIVKDMNAAGPIDPRISDESGYIRLRLGEVTVDGGRDEAYRLAVSSDGVEIEGKS
                     PHGIFNGAQTLLQLARDGAIIDGCAVTDRPAFSWRGYMVDVARNFHSMDLLKQQIDIM
                     GRYKLNVFHFHLTEDIAWRIAIQKYPPLTAPNTMLRDAGSFYCEEDIRELIGYCNERH
                     IVFLPGD"
     gene            1719..2204
                     /locus_tag="VKU83_11230"
     CDS             1719..2204
                     /locus_tag="VKU83_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94178.1"
                     /translation="MPGHSGAFARALKVDMQSPAGLAAVKAILRELVVTYHFPFIHIG
                     GDEVRITDTTFLPSVDPLESVVTIFNRQIGGVDARTARIIGATLCLWNDRAVASENDL
                     LRMNPVYPGMLAFAERSWRGGGKSGWVTGIGEPGTKRAAAFAEFEGRLLDQRRENLAG
                     L"
BASE COUNT          508 a          630 c          675 g          499 t
ORIGIN      
        1 acggaggcgc cagctgggat acggcctatt tcgacagcca cctcgccgac ccggtgtgcc
       61 agggaagtat tttcgccgta ggcagggaaa aaggccattc cattatcgcc gagtgtaata
      121 atgatgacag cgtgcaacgg aacaacctga cgcttcgcat cagctacgat gaggggagat
      181 cctggacccg cccgcaagtg gtcgatcgca gcgacgaggc gtcgaaaaaa aaggactaca
      241 ccgcttattc ggacatcgtc ggcctgtcgg ccaaaaccat cggggtactg tatgaacgga
      301 ataactacaa agagatcacc ttttccatcc tgaaatggcg atgatgaaaa cgatacgttg
      361 tagcctcctg ttgctggccc ccttgttgtt gcatgcccaa acccggctgc ccgcttaccc
      421 ggattctctt ttcccgacct actaccacca gcgggtgacg cattttcaaa cgctgcaccg
      481 gagctgcacc gatattttgt ttttgggcaa cagcatcacg gatggcgcgg aatggagcga
      541 actattcaac gatgcccgga tccgaaaccg gggcatcagc ggcgatatta cggccggggt
      601 gctccaccgg ttagcttacc tggccggggg aacgccggag aaagtgtttt tgctcatcgg
      661 cattaacgat ctctcccgcg ggacttcgcc ggacagtgtg gtcaaaaata ttctgctcat
      721 cgccgactat ctgcatgagc aaacgccggc cacccatctt tatgtgcaaa gcctgttgcc
      781 ggtgaacgat gggttcggga agttcggcac acataccaac aaaggccggg atatcctggt
      841 cgtcaaccgc tccttgcaaa atgacgccga aagtcaccac tattcttttg tcgatctgta
      901 cagttcgttt tgcgattcgg ccggcaagct acggtccgct ttcaccaacg acggacttca
      961 ccttaccggc gagggatacc tcctttggaa acatctcctg tatgcggatg tttttgacct
     1021 ggcggagaaa ccatcgctga ttcccctccc atggcagttg cagtggaggc cgggctattt
     1081 ttggttgagt cattgtgcag gcatagcggt caacgacagt gcgttgataa gggaggccag
     1141 atttctgcag gaggaactgt cccgaaaggg catccggcta cccatagtca aggacatgaa
     1201 cgccgcggga ccgatcgacc cccgcatttc cgatgagagt ggctatatcc gcttacggct
     1261 tggcgaagtg acggtcgacg gaggacgcga tgaagcctac cgcctcgccg tttcctccga
     1321 tggggtagag atcgagggaa aatccccgca tgggatattc aacggcgccc agacgctgct
     1381 gcagctggcg cgggacggag ccatcatcga cggttgtgcc gtcacggaca ggccggcttt
     1441 ttcctggcgg ggctatatgg tggatgtcgc acgcaatttt cactcgatgg acctgctgaa
     1501 acaacaaatc gacattatgg gccgctataa gcttaatgtc tttcatttcc atctgacgga
     1561 ggacatcgcc tggcggatag ccattcaaaa atatcctccc ctcaccgccc ctaatacgat
     1621 gttgcgcgat gccgggagct tttactgcga ggaggatatc cgggaactga tcgggtattg
     1681 caacgaacgg cacatcgtat ttcttcccgg agattgacgt gccggggcac agcggtgcgt
     1741 ttgcgcgagc gctgaaggtg gacatgcagt cacctgccgg gctggcggca gtgaaggcga
     1801 tcctccgcga gctggtggtc acttaccatt ttccttttat tcacattggc ggggacgagg
     1861 tgaggattac ggatacaacg ttcctgccta gtgtggaccc attggagagt gtggtgacga
     1921 tcttcaaccg gcagataggt ggtgtcgatg cccggactgc aaggatcatc ggcgccacac
     1981 tttgcctatg gaacgaccgt gccgtcgcga gtgaaaacga tctcctgcgg atgaaccccg
     2041 tgtatcccgg aatgctcgcc ttcgcggagc gcagctggcg gggtggtggg aagtcggggt
     2101 gggtcactgg aattggcgag ccgggtacga aaagggcggc ggccttcgcc gaattcgaag
     2161 gccgcctatt ggatcagcgc agggagaatc tcgccggcct gtaggcagct tcaaagggaa
     2221 agacaatcag aacccggtga agtggatgtt cacattcgtg ccctgccggg aataaggccc
     2281 gcatcggtaa tctatcaatg ccatcgtttc cc
//
LOCUS       DATGKE010000663         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1161044, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000663 DATGKE010000000
VERSION     DATGKE010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161044"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VKU83_11235"
     CDS             <1..1325
                     /locus_tag="VKU83_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI94179.1"
                     /translation="IVCQEMRHPNLPRNPVNRILLASLVGTTIEFFDFYIYATAAVLV
                     FPKLFFPASNATSATLESLATFALAFFARPLGAALFGHFGDRKGRKATLVAALMTMGP
                     STVAIGLLPTYHSIGIAAPILLAVFRFGQGLGLGGEWGGAVLLATENAPEHKKSWYGM
                     FPQLGAPVGFILSSGTFLLLGRFLSDRQFFNYGWRIPFIASAILVWVGLYVRLKLTET
                     PDFLRVMKNNERVKMPVADVFLKHGKAIALGTAAAITTFLLFYLMTVFTLSWGTSVLH
                     YPKAGFLAAQILSMLSFALGIPLSAMLADKYGRRNILLLATAGILVFGLFFSAFFSTG
                     SLAVTAVFLTVGMFLMGLTYGPLGTALAEIFPAAVRYTGSSLAFTLAGILGASLTPYL
                     ATTLAKSYGLSAVGYYLAAAAIVTFSALLGMSPARKDEPVLQQDFLRH"
     gene            complement(1299..>2092)
                     /locus_tag="VKU83_11240"
     CDS             complement(1299..>2092)
                     /locus_tag="VKU83_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HLI94180.1"
                     /translation="TLNNVYINIAGTLNFDQNKTLALDINSVVNIQSGGTLTATHPTP
                     NELLSINGVTKYDGKNDGTISGPVAATATTGSSPSGFTMVTLPVDFVSFSATRGNGTV
                     QLVWNTTNEINNSHFEIERSANGTSWEAIGDVAAGTPSLDHSYTYTDEAAPAAQAQYR
                     IRQVDIDGNYQYSKVVVVDGAVKATAEATIVVSGKTLSILNANATGNRIVVRLIAISG
                     QVLQQQSFEGNSGRIDMHVDASATGVYVVQVTDGNQWSVAKEVLL"
BASE COUNT          384 a          603 c          572 g          533 t
ORIGIN      
        1 caatagtttg ccaggaaatg cgtcatccta acctcccgcg caaccccgtc aaccgaattc
       61 ttttggccag cctggtgggc acgacgatcg agtttttcga cttttatatc tatgccacgg
      121 ctgccgtgct ggtgtttccc aaattgttct ttccagcgtc gaatgccact tcggcaaccc
      181 tcgaatccct ggcgaccttc gcgctggcct ttttcgcccg gccgctggga gcggccctgt
      241 ttggtcattt tggcgaccgt aagggccgaa aagcgacgtt ggtcgcagca ttgatgacca
      301 tgggaccgtc taccgtggcg ataggacttt tacccactta tcattcgatc ggcatcgctg
      361 cgccgatcct gctggcggta ttccggtttg gccagggcct cgggcttggc ggcgagtggg
      421 gcggcgcggt tctgctggcg accgaaaacg caccggaaca caaaaagtcg tggtacggca
      481 tgttccccca gctaggagcg cccgtcgggt ttattctatc cagcggcacc tttctccttc
      541 tcggcagatt cctgagcgac cggcaatttt ttaactacgg ctggcgcatc ccttttatcg
      601 ccagcgccat cctggtatgg gtaggacttt atgtccgcct gaagttaacg gagacccccg
      661 actttttaag ggttatgaag aataacgaac gggtgaagat gcctgtcgct gacgtcttcc
      721 ttaagcacgg caaagcgatt gccctcggca cggcggcagc catcactacc tttctcttat
      781 tttacctgat gacggttttc acgctgagtt ggggcacttc cgtgctgcat tatccgaagg
      841 cgggtttttt ggccgcccag atcctgtcca tgctttcctt tgcgctaggc atccccttat
      901 ccgcgatgct cgccgataag tatggccgcc gcaacattct gttgcttgcc actgcgggaa
      961 tccttgtttt cggcctgttt ttttcggcgt tcttcagcac gggcagcctg gccgtaaccg
     1021 ccgtctttct caccgttggg atgtttttaa tgggcctgac ctatgggccg ttggggacag
     1081 ccctggccga aatctttccg gcggccgttc gctacaccgg ttcttcattg gccttcacgc
     1141 tggcggggat cctcggggcc tctttgacgc cctacctggc gactaccctt gcgaagagct
     1201 acggactttc tgcagtagga tattatcttg ccgcggcggc gatcgttact ttctcggcgt
     1261 tgctgggcat gagcccggcc cggaaggatg aaccggttct acaacaagac ttccttcgcc
     1321 actgaccatt ggttaccatc ggtcacctgt acaacataga cgcccgtagc ggaagcgtca
     1381 acatgcatat cgatccggcc cgaattaccc tcgaaggact gctgctgcag tacctggccg
     1441 ctgatcgcga tcagcctgac gactatccgg ttgccggtgg cgtttgcatt taagatagac
     1501 agcgttttgc cgctgacgac gattgtagcc tctgcggttg ctttcacggc gccatcgacc
     1561 accacgacct tgctatactg gtagttgccg tcgatatcca cctggcgaat gcgatattga
     1621 gcctgggcgg ccggtgcagc ttcatccgta taggtatagc tatgatccaa agaaggggta
     1681 cctgcggcaa catcgccgat cgcttcccag cttgtgccat tggcgctgcg ttctatttca
     1741 aaatgactgt tgttgatctc attggtcgtg ttccagacca actgcacggt cccgttgccg
     1801 cgagtggcgg aaaaggagac aaaatctacc ggcagcgtca ccatcgtaaa tccggagggg
     1861 gaagaaccgg tcgtcgcggt ggccgcaacc gggccggaaa tggtaccatc attcttaccg
     1921 tcatacttcg tgacgccgtt gatgctgagc aactcgttgg gcgtagggtg cgtcgccgtc
     1981 agcgtaccgc cgctctgaat attgacgacc gaattgatgt cgagcgccag cgtcttgttt
     2041 tgatcaaaat taagcgtgcc tgcaatattg atatagacgt tgttcagcgt at
//
LOCUS       DATGKE010000664         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_975851, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000664 DATGKE010000000
VERSION     DATGKE010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975851"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VKU83_11245"
     CDS             complement(<1..186)
                     /locus_tag="VKU83_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94181.1"
                     /translation="MGVATIKPFLRLAGLEPLVMRPQTNFVNIGERTNVTGSKKFARL
                     IRDNKFEEALSVARQQVE"
     gene            complement(201..1244)
                     /locus_tag="VKU83_11250"
     CDS             complement(201..1244)
                     /locus_tag="VKU83_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLI94182.1"
                     /translation="MTNTRQLIEKALHERILVIDGAMGTMIQRHKLGESDYRGERFKD
                     WPSDLKGNNDLLVLTQPAIIEGIHREYLEAGADIIETNTFNAQRISMADYHMEDLAFE
                     INAAAARLAKKAAAEYTARNPAKPRFVAGAIGPMNKTLSLSPDVNNPGFRAVSFDEVA
                     SAYYEQVKGLVEGGADLLLIETIFDTLNAKAAIYAIKKYFRDEAHEGGVPHELPVMIS
                     GTITDASGRTLSGQTLEAFYVSVMHARPLSIGLNCALGAAQMRPHIEELSQIAACFTS
                     AYPNAGLPNAMGEYDERPEDTAHFIEEWARNGFVNIVGGCCGTTPDHIRHIADHIAGV
                     KPRPLPVLEKELV"
     gene            complement(1630..2250)
                     /gene="recR"
                     /locus_tag="VKU83_11255"
     CDS             complement(1630..2250)
                     /gene="recR"
                     /locus_tag="VKU83_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLI94183.1"
                     /translation="MQFSSVLLENAVAEFARLPGIGKKTALRLVLHLLRSDSAQAQGL
                     SEAVVRMRQEIKFCQRCHNVADGDICSICANSFRKREMICVVETLREVIAIESTQQYN
                     GVYHVLGGIISPLDGVGPEQLNIESLVARVRKEKPQELIFALNPTIQGDTTIYYIQKK
                     LQDIPLRITTIARGIAFGGELEYADEMTLARSISNRLPVESYRAGG"
     gene            complement(2260..3678)
                     /locus_tag="VKU83_11260"
     CDS             complement(2260..3678)
                     /locus_tag="VKU83_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HLI94184.1"
                     /translation="MKLRNALLLFPLSFLVGCASIVPPTGGPRDTLPPVLVSSVPVEF
                     SRHISSNKIVFKFDEFIDAKDIRGELVVNPVPKVDPITDGHLQTVTIRLKDTLLPNTT
                     YTLNFYKGIKDVNEGNILRDFTYVFSTGDYIDSGRLGGTVLLALTGKADSSLVVILHK
                     KLDDSAVIKDRPRYLTRVDTTGRFLFDHIEPGRYAVYALKDEGGSHKYLSKSQLFAFA
                     DGPVDVTAGTMPVTLYAYSEQPETPSGSRNSSSTGTQPKPSKKPKEKERLEVKENTTN
                     GTFDILDTFRLSFGTGLKLFDSALIRFTDANFKDIGLPQYRWVRDSTNKNFYLAYSWP
                     LDTKYNLILPKAAMADSAGRRLLKDDTITFRTKKDIDYGEIRIRVFNLDLSRRPVLLF
                     YSSDKLKYSIPFGTKKEIRQLLFEPGDYEVRILYDTNGNGKWDPGQFFGKHRQPERVI
                     SVLKKFTVKANWDNDRDISLSP"
     gene            complement(3735..>5226)
                     /locus_tag="VKU83_11265"
     CDS             complement(3735..>5226)
                     /locus_tag="VKU83_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLI94185.1"
                     /translation="EESRRNPDLDLEKLVQTVDLMKQNGLTVPLFQVSGSEKAVNLLT
                     AHGSKGLEFEYVFFAGCNAAIWEKKRRPGGGYRYPDTLFARVGGNGAAGISARGNGAA
                     ANGEPASGAASGARAAADASAADPAFANASTTEEELRRLFYVALTRVERHLSISWSRF
                     RDDGRELEPSVFIAEILDGHLLPIEKVVIPAEDLAAFDALPFAEVVAPEIRSAEEDFI
                     TGALEKFVMNVTALNNYLKCPLEFYYRNLIRIPSPKNEATEFGSAVHFALQRLFEKMK
                     AHAAEAFPEKAELLNDFKWYMGRHREIFTREAFARRMEYGLEVLANYYDKYVHQWNRI
                     VTVERNIRNVVLRGVPLKGKLDKLEFQGKEVTVVDYKTGDVDKAKEKLAPPGPSAPHG
                     GDYWRQAVFYKILVDSYEQKDWKVTGTEFDFVEPDKRKEFRKVKLIISPEDITTVTRQ
                     IVTVWEKIQNREFYTGCGKADCHWCNFVKTNNLAIALHEVDDGEEI"
BASE COUNT         1117 a         1511 c         1359 g         1239 t
ORIGIN      
        1 ttccacctgc tgacgggcca cactcagcgc ttcctcaaat ttgttgtccc ggataagacg
       61 cgcgaacttc ttcgaacccg tcacgttcgt gcgttccccg atgttgacaa aattcgtctg
      121 gggcctcatt accaggggct cgaggccggc gagccttaaa aaaggtttga ttgtagcaac
      181 tcccatctgt tatttttagg ttagaccaac tctttttcca atacgggcag cggcctgggt
      241 ttgacaccgg caatatggtc ggcaatgtgc cggatgtgat cgggcgtcgt accgcagcag
      301 ccgcctacaa tatttacaaa accgttgcgc gcccactcct cgatgaaatg ggccgtgtcc
      361 tcgggccgct cgtcatattc gcccatagcg ttcgggaggc ccgcgttcgg ataggctgaa
      421 gtaaaacatg ccgcgatctg gctcagctcc tcgatatgcg gccgcatctg ggccgccccc
      481 agcgcacaat tcagtcctat gctcaacggg cgcgcatgca tgaccgagac atagaaggcc
      541 tccagcgtct gaccgctaag cgtccggccg cttgcatcgg tgatcgtccc gctgatcatc
      601 acaggcagct cgtgcggcac gccaccctca tgagcctcgt cccggaagta tttttttatt
      661 gcgtaaatag ccgccttggc attcagggtg tcgaagatcg tttcgatcag cagcagatcg
      721 gctccccctt ccaccaggcc ctttacctgt tcgtaatagg cggaagcgac ctcgtcaaag
      781 ctgacggcgc ggaaacccgg attattgacg tccggggaaa gcgaaagcgt cttgttcatc
      841 ggtccgatcg cacctgcaac gaaccggggc ttggccgggt tgcgggcggt atattccgcc
      901 gccgcttttt tcgcgaggcg ggccgcggcc gcattgatct caaatgcgag gtcttccata
      961 tgataatcgg ccatgctaat ccgctgggca ttgaaagtgt tcgtctcgat gatatcggcg
     1021 cccgcttcca ggtattccct gtgaatgcct tcgatgatgg caggctgcgt caggacgagc
     1081 aagtcgttat ttcctttaag gtccgatggc cagtccttga accgctcgcc gcggtaatcg
     1141 ctttcgccca gcttatggcg ttggatcatc gtgcccattg cgccgtcaat gaccagtatc
     1201 cgctcgtgca atgccttttc gataagttgt cttgtgttcg tcatttgatc ctcctttctt
     1261 ttgaattttc cgtccggagg tcaaacgctt ttttccgtaa aaaattttgt gaactgctga
     1321 taacgaggga aataacaatt gaaaaagaga aggagttatt tcagccgttt attagttcag
     1381 tgatttctac ttcgaagcgc cggctccggc cagacaggct gaacaataaa caaatccgcc
     1441 tgtatggacc gcaaatatac agcaaatatg tttaggatca ataggctttc gcctgcgggc
     1501 gggtgggcgt ttgggtgggg cgaggtatcg cagaaatggc cggggcaagg tgtttcagaa
     1561 cttaattggg ccgaaatcgg ccccattaag gggaagggta tggccagggg gtcatcctcc
     1621 cgccagggct caacctcccg cccggtagct ttccaccggc aacctgttgc tgatcgaccg
     1681 cgccagcgtc atctcatcgg catactctaa ttccccgcca aaggcgattc cgcgggcaat
     1741 cgtggtgatc cgcaacggga tatcctgtaa ttttttctgg atatagtaaa tagtcgtgtc
     1801 tccctgaatg gtgggattca acgcaaagat caattcctgc ggcttttctt tccgcaccct
     1861 ggcgaccagc gattcgatat tcagctgttc agggccaacc ccgtccagcg gcgagataat
     1921 cccacccaaa acatgataga caccattata ttgttgcgta ctctcgatcg cgatcacttc
     1981 ccgcagcgtc tccaccacgc aaatcatctc ccgcttccgg aaggaattgg cgcagatgga
     2041 acagatatcc ccgtcggcga cattgtgaca acgctggcag aatttgatct cctgccgcat
     2101 ccgaaccacg gcctcgctca gcccctgcgc ctgggccgaa tccgaccgca gcaaatgcag
     2161 gaccagccgc aaagcggttt ttttcccgat ccccggcagc cgggcaaatt cggcaacggc
     2221 attctccagt aaaacagaag aaaattgcat cctgtaaagt tacggcgata agctgatatc
     2281 ccggtcattg tcccagttcg ctttgaccgt gaatttctta aggacgctga tgactctttc
     2341 gggctgccgg tgctttccga aaaactggcc gggatcccac tttccattgc cgttggtatc
     2401 atataagatc cttacctcgt agtcgcccgg ctcgaacaac aactgccgga tctccttttt
     2461 cgttccaaat gggatggaat atttcaattt atcagacgaa taaaaaagca aaacgggccg
     2521 ccggctcaag tccaggttga agaccctgat ccggatctcc ccgtagtcga tatccttttt
     2581 tgttctgaac gtgatcgtat catccttcaa cagcctcctg cccgccgaat cggccatcgc
     2641 cgcctttgga aggatgagat tgtactttgt gtccaacggc caggaataag ccaggtaaaa
     2701 attcttgttg gtcgaatccc gaacccaccg gtactgcgga aggccgatat ccttgaaatt
     2761 ggcatctgta aaccggatca gcgccgaatc gaagagtttc agtccggtac caaaacttaa
     2821 ccggaaggtg tccaggatat cgaatgtccc attggtggtg ttctccttta cctccagccg
     2881 ctccttttcc ttcggttttt tcgacggctt tggctgtgtg ccggtgctgc tgctattccg
     2941 gcttccggac ggtgtttccg gctgttccga ataagcatac agcgtgacag gcatggtccc
     3001 ggccgttaca tcgaccggtc cgtccgcaaa agcgaacagt tgcgatttgg agaggtattt
     3061 gtgcgaaccg ccttcatcct tcaaggcata gaccgcataa cgtcccggct cgatatgatc
     3121 gaagaggaac cgcccggtag tgtccacccg cgtcaggtat cgaggccggt ccttgatcac
     3181 ggccgagtca tccagttttt tgtgcaggat gaccaccaaa ctcgagtccg ccttgccggt
     3241 cagggcgagg agcacggttc cccccagccg gccggaatcg atataatcgc cggtggaaaa
     3301 gacataggta aaatcacgca gaatatttcc ttcattgaca tccttgatgc ctttatagaa
     3361 attgagggtg taggtcgtat tcggcaaaag tgtgtctttt aaccggatgg tgacagtctg
     3421 gagatgacca tccgtaatag gatcaacctt tgggaccggg tttaccacca gctccccccg
     3481 gatatccttg gcatcgataa attcgtcgaa cttgaacacg atcttattgc tgctgatatg
     3541 cctgctgaac tctacgggaa ccgaactgac gagaacgggg ggaagcgtat cccggggccc
     3601 gccggtaggc ggaacgatac tggcgcaccc gacgagaaaa gaaaggggga aaagcaatag
     3661 cgcgtttcga agcttcatct tgcaaacata atgggttttt gagcgggcat cctatcttaa
     3721 ctcctaataa tcctttaaat ctcctcgccg tcatccacct catgaagggc gatcgccaaa
     3781 ttattggtct tcacaaaatt gcaccagtgg caatccgctt tgccgcagcc cgtataaaat
     3841 tccctgttct gaatcttttc ccagacggtc acgatctgcc gggtgaccgt cgtaatatcc
     3901 tccgggctga tgatcagttt gactttgcgg aattcctttc ttttatcggg ctccacgaaa
     3961 tcgaattccg tcccggtcac cttccaatct ttctgctcat agctatccac caggatcttg
     4021 tagaacacgg cctgccgcca gtaatcgcct ccatggggag cgctggggcc ggggggagcg
     4081 agcttttcct tcgccttgtc cacatcgccg gttttgtagt ccacgacggt cacctccttc
     4141 ccctggaatt ccagcttgtc cagcttgccc tttaagggca caccccgcaa gacgacgttg
     4201 cggatatttc gctcgacggt cacgatccgg ttccactgat gaacatattt gtcgtaatag
     4261 ttggcaagca cctccagccc gtattccatc cggcgcgcaa acgcctcgcg cgtaaaaatc
     4321 tcccggtgcc ggcccatata ccatttgaaa tcgttcagga gctccgcctt ttcgggaaat
     4381 gcctccgccg cgtgcgcttt cattttttcg aacaggcgtt gtaacgcaaa atggacggcg
     4441 ctgccgaact ccgtcgcctc gttcttcggc gagggaatcc ggatcaggtt cctgtaatag
     4501 aattccagcg ggcacttgag gtagttgttc agggccgtca cgttcatcac aaacttctcc
     4561 agcgctccgg tgatgaagtc ttcttccgcc gaccggatct cgggcgcgac cacttcggcg
     4621 aacggcaggg catcgaaggc tgccaggtcc tccgcaggaa tgacgacctt ttcgataggc
     4681 aacaaatgac catcgaggat ctcggcaata aagacagaag gctccaactc cctgccgtcg
     4741 tcccggaacc gcgaccagga gatggataaa tggcgctcga cccttgtcag tgccacgtag
     4801 aacaagcgcc gcagctcctc ctcggtcgtc gaagcgttag cgaaggcggg atcggctgct
     4861 gaagcgtcgg cggcggcgcg ggccccggag gcggcaccgg aagccggttc gccatttgcg
     4921 gccgcgccat ttccccgggc gctgattccc gcggcgccgt ttcccccgac ccgcgcaaaa
     4981 agggtatcgg gataccgata gccgccgccg ggccgccgct tcttttccca gatagcggca
     5041 ttgcaaccgg caaaaaagac gtattcgaat tcaaggccct ttgacccatg cgccgtcagc
     5101 agattgactg ccttttcgct gccagagacc tggaacaggg gcacggtcag gccattttgc
     5161 ttcatcaggt cgacggtctg gaccagcttc tccaggtcaa ggtccgggtt gcgtcgcgac
     5221 tcctcc
//
LOCUS       DATGKE010000665         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_562959, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000665 DATGKE010000000
VERSION     DATGKE010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562959"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VKU83_11270"
     CDS             complement(<1..422)
                     /locus_tag="VKU83_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLI94186.1"
                     /translation="MWIAGKLQRTRSLFDKRYQELNRLSRLPRYTPGSFEWKGTSIAF
                     PDSSSFVFMYKELFVGNIYKFNSDIESPLILDCGANIGMSVIYLKELFPKARIIAFEP
                     ERKIFQFLQKNTAAFGLKDVKLVNKAVWKEEGTIRFSN"
     gene            complement(439..1563)
                     /locus_tag="VKU83_11275"
     CDS             complement(439..1563)
                     /locus_tag="VKU83_11275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLI94187.1"
                     /translation="MSGTTPYKKLYYTNLDTIRCFAAFFVVVYHWFPGKMTDSPLAKI
                     GVDVFFVLSGFLISEILFKARDEAGDNPLLKGRAIGVFYMKRALRIFPIYYLLVSFFF
                     WMNQPPLGGNWTYVFTYTTNFLIYWKQAWIYPLSHLWSLGVEEQFYIVWPFFIFFLPN
                     KRLLQLIVFVLAVSVLFILIDEPRNPFFFVLPFSCMTTLGAGALLAYIKLRRGEAFRK
                     FARYTLPVTVAAAASLVFWKRFNFITLHLVVAVMAFSLIWYAITSRSRVFNTVFSNPV
                     TSYLGKISYGIYLYHHPIPWLVRSLNGTETAVVRHLPVFLPAFRNGYAILAENLAVLL
                     IVASLSWFLIEKPLNSLKARLGKRDQAGLSLAATPPGSIR"
     gene            complement(1583..2140)
                     /locus_tag="VKU83_11280"
     CDS             complement(1583..2140)
                     /locus_tag="VKU83_11280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLI94188.1"
                     /translation="MGMIRRLSDLFRRAGFAWRRFRLMLNKKIVLGKNCHIYAGCRIM
                     TNNGGQIGIGSGSEILFGVCLMTYGGRIAVGDRCSINPYTVIYGHGEGTLIGNDVLIA
                     GHCLIIPANHVFGDVSLPINQQGVSSKGIVIEDNVWIGSGCRILDGVRIGKGAIIAAG
                     SVVNRDVPANTIVGGIPAKPIRTRN"
     gene            complement(2131..>2362)
                     /locus_tag="VKU83_11285"
     CDS             complement(2131..>2362)
                     /locus_tag="VKU83_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94189.1"
                     /translation="REQDFGQGSIVKKMFIPSHLLLTGSYSLDVASFIPNSMTYTYLQ
                     DVCPFKVQDFESELAIYGTNDVGVVNINCEWV"
BASE COUNT          579 a          608 c          642 g          533 t
ORIGIN      
        1 tcattgctaa agcgtatcgt gccctcttct ttccatacgg ctttattgac aagcttcacg
       61 tctttcagac cgaatgccgc cgtattcttt tggaggaact ggaagatctt tcgttccggc
      121 tcgaaagcga tgatacgtgc cttcgggaat agctctttca gatagatgac actcatgccg
      181 atgtttgcgc cgcaatcgag gatcagcggg gattcgatgt cgctgttgaa cttatagata
      241 tttcctacaa aaagctcctt atacatgaac acaaaggagg acgaatccgg aaaagcgata
      301 ctcgtccctt tccattcgaa gctgccgggg gtatacctcg gaagccggga cagccggttc
      361 agctcctggt aacgtttgtc gaaaagcgat ctggtccttt gcaatttacc tgcgatccac
      421 atgatgatta tatttcattt atcgaatgct gcctggcggt gtcgccgcca gggagaggcc
      481 ggcctggtct ctttttccca gccgggcttt cagcgaattg agcggtttct cgatcaggaa
      541 ccaggaaagg gaggcgacga tgagtagaac ggcgaggttc tccgccaata tcgcgtatcc
      601 gttcctgaaa gccggcagga aaaccggcag atgcctgacc accgcggtct cggtcccatt
      661 gaggcttcta accagccacg gtatcggatg gtggtacaga tagatcccat agctgatctt
      721 gcccagatag ctggtgaccg ggttcgaaaa tacggtattg aaaacgcggc tccggctcgt
      781 aatggcatac caaatgaggg agaaggccat tacggcaacg acgaggtgca gcgttatgaa
      841 attgaaccgt ttccaaaaga ccaaagacgc cgcggccgcc acggtgacgg gcaaggtata
      901 gcgagcgaat ttccggaatg cctctccgcg tctcagcttg atgtaggcca gcagggcgcc
      961 tgctccgagt gtggtcatac agctgaaagg caagacgaaa aagaaggggt tccggggctc
     1021 atcgatcagg atgaacagca cgctaacggc caggacgaag acgatgagct gcaacagtct
     1081 tttattcggc aggaagaaaa taaaaaaggg ccacacaatg tagaactgct cttccacgcc
     1141 cagtgaccac aaatgggaga gcgggtagat ccaggcctgt ttccagtaga taaggaaatt
     1201 ggtagtatac gtaaagacgt aggtccaatt cccgccgagg gggggctggt tcatccaaaa
     1261 aaagaaggac accagcaggt agtagatcgg gaagatccgg agggcgcgtt tcatatagaa
     1321 cacgcctatc gcccgtcctt tcagcagggg gttatcgcct gcttcgtcgc gcgccttgaa
     1381 taatatttcg gagattagaa agccgctcag gacaaaaaag acgtccacgc ctatttttgc
     1441 caggggcgaa tcggtcattt ttccgggaaa ccaatggtag actaccacga aaaaggcggc
     1501 aaagcacctt atggtatcca ggttggtata atatagcttt ttatatggcg tcgtaccgga
     1561 cataaacgat gcggtattgg ttttaattcc gtgttctgat cggtttggcc gggatcccgc
     1621 ctacgatggt attggcggga acgtcccggt tgacgacgct gcccgcggca ataatggcgc
     1681 ctttcccgat cctaacccca tccaggatgc ggcatcccga gccgatccag acgttgtctt
     1741 cgatgacgat ccccttgctg ctaacgcctt gctggttgat gggcagggat acatccccaa
     1801 agacatggtt cgccggaatg atcagacaat gcccggcgat cagtacgtcg ttcccgataa
     1861 gggtcccttc accgtgtccg tagatcaccg tatagggatt aatgctgcat cggtcgccga
     1921 cggcgatccg gcccccgtag gtcatcaggc atacgccgaa caggatctcg ctccccgaac
     1981 cgatgccgat ctgtccgccg ttgttggtca ttatcctgca accggcatag atatggcaat
     2041 tcttaccgag gacgatcttt ttgttgagca tcagcctgaa cctgcgccag gcaaatccgg
     2101 cccgtctgaa aagatcgctg agccgccgga tcatacccat tcacagttga tgttcactac
     2161 gccgacgtcg ttggtgccgt aaatggcaag ctccgactcg aaatcctgca ccttgaacgg
     2221 gcaaacgtcc tgaagatagg tataggtcat gctgttgggg ataaagctcg cgacatcgag
     2281 gctataggat ccggtcaaaa gaagatgcga agggatgaac atttttttaa cgatgctccc
     2341 ctggccgaaa tcctgttccc tg
//
LOCUS       DATGKE010000666         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_874609, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000666 DATGKE010000000
VERSION     DATGKE010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874609"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VKU83_11290"
     CDS             <1..851
                     /locus_tag="VKU83_11290"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLI94190.1"
                     /translation="THESIRTVRPLKDGVIADFNAAELMIREMIKMIYPKKPLFPPSW
                     RMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIIDI
                     GGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQIGA
                     ALKDLDNPPDDIPVNGRDLVTGIPKQIMVSYQEIAEALDKSIFKIEEAILKALETTPP
                     ELAGDIYRRGLYLTGGGALLRGLDKRLSQKIKLPVHIADDPLKSVVRGTGIALKNYDR
                     YPFVMR"
     gene            887..1732
                     /gene="mreC"
                     /locus_tag="VKU83_11295"
     CDS             887..1732
                     /gene="mreC"
                     /locus_tag="VKU83_11295"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLI94191.1"
                     /translation="MRNVFLFIRRHFNFLFFLVLQIVALSFLFRYNKFHEAAFLSVST
                     ELTGRLNAKYNNIEYYFQLRKTNEALVQENLQLRQQLRDNFEAPDTAQRLFRDTIRTD
                     SVRKWEVMEAKVVSNTIALPANYLTIHRGFKQGVRANMGVAGPKGLVGQVINVSENFA
                     IVMSMLHPQFKVIARLKKGGDNGTVFWDGLSPNYFTMRGIPRSANVAVGDTVLTSQIT
                     YLFPANMMVGTISEIIPDNTTNFYTLKLRSATNFSNLEYAYVLDNLQAGEQRRLEDST
                     RKKLQ"
     gene            1729..2262
                     /locus_tag="VKU83_11300"
     CDS             1729..2262
                     /locus_tag="VKU83_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94192.1"
                     /translation="MSELLKNIIRFVLFILVQVFILSRMPPLHRFITPYLYYLYILWL
                     PFSLSRMSLTFIGFVYGLCLDYFLKTPGLHAAACTLIAYVRPFIIGMLISQEGADKNY
                     VAPSFVSMGWAPYGTYVLVLTLLHHSYLVLLEWMNFGTFVYFVGKVIASTAVSLLLVL
                     LTEILFYRKQKFRTNTI"
     gene            2420..>2623
                     /locus_tag="VKU83_11305"
     CDS             2420..>2623
                     /locus_tag="VKU83_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94193.1"
                     /translation="MSVFNQSRSNVIRLIFAGMFLIIAAQLFHLQIISKQYKEAAQKN
                     ALFKKSIYPTRGIIYDRKGRAILN"
BASE COUNT          611 a          755 c          682 g          575 t
ORIGIN      
        1 aaacccatga aagcatccgc acggtccgcc ccctgaagga cggtgtcatc gccgacttca
       61 acgcggcgga gctcatgata cgggagatga tcaagatgat ctatcccaaa aaaccgctgt
      121 tcccccccag ctggcggatg atgatctgca tcccttccag tatcacggag gtggagaaac
      181 gggccgtccg cgacagcgcc gaacaggccg gggccaagga agtgtacctg atccacgaac
      241 cgatggcagc ggcgctgggc atcgggatcg acgtcgagga gccggtgggc aatatgataa
      301 tcgatattgg cggcggtacg acgggcatta ccgttatcgc cctggcgggt atcgtgtgcg
      361 accagtcgat ccgcatcgcc ggcgacgaat ttaccgccga catcatggag gcgctgcgcc
      421 gctaccactc gctgctcatc ggcgaaagga cggcagagca gatcaagatc cagatcggcg
      481 ccgccctgaa ggacctcgat aatcccccgg atgatattcc ggtcaatggc cgggacctcg
      541 tgacgggcat cccgaaacaa atcatggtat cctaccagga gattgccgaa gcgctcgata
      601 aaagcatctt caaaatagag gaagcgatcc tcaaagcgct ggaaaccacc ccgccggaac
      661 tggccggaga catttaccgg cgcgggcttt atctgaccgg cggcggcgcc ttgctccgcg
      721 gcctcgacaa acggctgagc caaaagataa aattacccgt acatattgcc gacgatccgc
      781 tgaaaagcgt cgttcgcggt accgggatcg ccctgaaaaa ttacgaccgc tatccgttcg
      841 tgatgcgata gacggtcgtg taaacctcga aataaaccag ctaccggtgc gtaacgtttt
      901 tctcttcatc agaaggcatt tcaacttcct cttcttcctg gtgttgcaga tcgtagcctt
      961 atccttcctc ttccggtaca acaagttcca tgaggctgcc ttcctgagcg tcagcaccga
     1021 actgaccggc cgcctgaatg ccaaatacaa caatatcgag tattacttcc agctcaggaa
     1081 aaccaatgaa gcgctggtgc aggagaattt gcagctccgg cagcagctgc gggacaactt
     1141 cgaagctccg gataccgccc agcggctttt ccgggacacg atccgtacgg actccgtacg
     1201 gaaatgggaa gtgatggaag cgaaggtcgt gagcaatacc atcgcgctgc ccgcgaacta
     1261 cctcacgatc caccgtggtt tcaaacaggg ggtgcgcgcc aacatgggcg ttgccggccc
     1321 gaaaggcctg gtggggcagg tgatcaacgt cagcgaaaac ttcgcgatcg tcatgagcat
     1381 gctgcatccg caattcaagg tgatcgccag gctcaagaag ggtggcgaca acgggaccgt
     1441 tttctgggac ggactgagcc cgaactattt caccatgcgg gggatccccc gcagcgccaa
     1501 tgtggcggtc ggcgacaccg tcctgaccag ccagatcact tacctttttc ccgccaacat
     1561 gatggtggga acgatcagtg agatcattcc cgacaatacg acgaatttct acaccctgaa
     1621 gctgcggtcc gccaccaatt tttcaaacct ggaatacgcc tatgtgctgg acaaccttca
     1681 ggcgggcgag cagcgccggc tggaggattc aacgcgcaaa aaacttcaat gagcgaactg
     1741 ctgaaaaata tcatccgctt tgtcctgttc atcctggttc aggtttttat cctttcccgg
     1801 atgccgccgc tgcatcgttt tatcacgcct tacctttatt acctctatat cctgtggctt
     1861 ccctttagcc tctcccggat gtcgctgacc ttcatcgggt tcgtttacgg gctatgcctg
     1921 gactattttt taaagacgcc gggcctgcat gccgccgcct gcacgctgat cgcctatgtc
     1981 cggcccttta tcatcggcat gctgatctcg caggaaggcg cggacaagaa ctacgtggcg
     2041 ccttcctttg tcagcatggg ctgggcgcct tacgggacat acgtcctcgt gctgacgctc
     2101 ctgcatcatt cctatcttgt actgctggag tggatgaatt tcggaacctt tgtgtatttt
     2161 gtcggcaagg ttatcgcatc taccgccgtc agcctgctgc tggtgttgtt gacggaaatc
     2221 ctcttctatc gcaaacaaaa gttccggacc aatacaatct gagtgccttt taacgtttaa
     2281 accgggcgaa gcccagtttg cacttccgga aggaagtgca gacgtagata gcaacgagag
     2341 aaaaagttaa attgcgtcgc caactttttc tatcgtttca aaatggcccg tagttccgta
     2401 aattgcaggt ttctccaata tgtctgtatt caaccagtcc cgcagtaacg ttatccgcct
     2461 catcttcgcc ggcatgttcc ttattattgc ggcccagctt ttccacctgc agatcatctc
     2521 caaacaatac aaagaggccg cccagaagaa tgcgctgttc aagaagagca tttacccgac
     2581 ccggggaatc atttacgaca gaaaaggaag ggcgatcctc aat
//
LOCUS       DATGKE010000667         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1498482, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000667 DATGKE010000000
VERSION     DATGKE010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498482"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VKU83_11310"
     CDS             complement(<1..567)
                     /locus_tag="VKU83_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HLI94194.1"
                     /translation="MKDRWIGFINDLQNRICAALEELDGKAVFILDEWERAEGGGGKT
                     RVLSGGRIFEKGGVNSSVVWGKVTDIMRSQLKIEADTWFACGLSLVIHPLNPYVPTTH
                     ANWRYFELYDKQGDLSDRWFGGGADLTPYYLFEEDARHFHRSFKAAMDPFDPAWYPKY
                     KKWCDEYFVNTHRGNEMRGIGGVFYDHLR"
     gene            complement(536..1603)
                     /gene="hemE"
                     /locus_tag="VKU83_11315"
     CDS             complement(536..1603)
                     /gene="hemE"
                     /locus_tag="VKU83_11315"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HLI94195.1"
                     /translation="MIQRAILKNDLLLRTLKGEKTERTPVWMMRQAGRYLPDYLKLKE
                     KYSFFERCQTPELAAEITIQPVEQVGVDAAIIFSDILVVPQAMGMEVQMVENKGPLLP
                     DPIQNVADLDRLTVPDVQERLGYVFEALRLTKQTLDGRVPLIGFAGAPWTLLCYMVHG
                     KGSKTFDEAKAFCYTQPKTAHRLLQLITDTTIAYLRGQAEAGADVVQIFDSWGGLLGP
                     DDFGTLSMPYIRQIVEAVKDKALTIVFAKGAWHSLESMASTGAHGLGIDWCIRPQLAR
                     QLAGPRVALQGNFDPAKLLSPVPVIRKEVAAMLDAFGDGPHIANLGHGILPNVPVDHA
                     RAFVDTVKNYQHEGPLDRVHQ"
     gene            complement(1600..2340)
                     /locus_tag="VKU83_11320"
     CDS             complement(1600..2340)
                     /locus_tag="VKU83_11320"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLI94196.1"
                     /translation="MPSSPAYVLSTRPLSPTLVDTAAANGVRIDILPFIAVEPLKLGA
                     GELPAGPSTAVFTSVNALAALENNAMTALKGGGGRAWKIFCLGGATWQKAAEYFGRAA
                     IAGTADSARELADEIVRQMPAEKASSGIDAGADRELWFFCGDRRRDELPDLLGQAGLK
                     VHEIFVYRTRLTPHRIGRNYDAIAFFSPSAVESFFSVNTLPPDIPLFAIGGTTAGAIR
                     RICRNPVTESERPDEETLIHQIIKNIQH"
BASE COUNT          487 a          742 c          636 g          537 t
ORIGIN      
        1 ccggagatga tcatagaaaa caccgccgat acccctcatt tcgttcccgc gatgtgtatt
       61 gacgaaatac tcatcgcacc attttttgta tttcgggtac caggccggat caaatgggtc
      121 cattgccgcc ttgaagcttc gatgaaaatg gcgggcatcc tcctcgaaca aataataagg
      181 tgtaagatcg gccccgcccc cgaaccaccg gtcgctgaga tccccctgct tgtcgtataa
      241 ctcgaaatag cgccaattgg catgagtggt gggcacataa gggttcagcg gatgtatcac
      301 cagcgatagt ccgcaggcaa accaggtgtc cgcttctatt ttaagctgag aacgcatgat
      361 atcggtcacc ttcccccaca ccacggagct gttcacgccg cctttctcga agatccggcc
      421 gcccgagagt actctcgtct ttccgccgcc gccttctgcc cgctcccatt cgtcgagaat
      481 gaagacagcc ttcccgtcca gctcttcaag agcggcgcag atcctgttct gcaagtcatt
      541 gatgaaccct atccagcggt ccttcatgtt gataattttt aaccgtgtcg acaaatgccc
      601 gcgcatggtc cacagggacg ttgggcagga tgccgtgacc taaattggcg atatgcggcc
      661 cgtcgccgaa agcgtccagc atggctgcca cctctttccg tatcacgggt acaggtgaaa
      721 gcagcttggc cgggtcgaaa ttcccctgaa gcgcgacgcg gggaccggca agctgtcgtg
      781 ccaattgcgg gcggatacac cagtcgatgc ccagcccatg agcgcccgtc gaggccatgc
      841 tttccagtga atgccaggcg cccttggcaa agactatggt gagcgccttg tcttttacgg
      901 cttccactat ctgccggata tagggcatgc tcaacgtccc gaaatcatcg ggccccagca
      961 ggccgcccca actgtcaaag atctggacga catcggcgcc ggcttccgcc tgtccccgca
     1021 gataggcgat cgtagtatcg gtgatcagct gcaggaggcg atgggccgtt ttcggctgtg
     1081 tataacagaa agcctttgcc tcgtcgaatg ttttggatcc tttcccatgc accatatagc
     1141 atagcaaagt ccacggcgca ccggcgaacc cgatcagcgg cactcttcca tccagcgtct
     1201 gtttcgtgag ccgcaaggcc tcgaatacat aacccagcct ttcttgcaca tcgggcaccg
     1261 tcagtctgtc gaggtctgct acgttttgaa tgggatcggg aaggagcggt cccttattct
     1321 cgaccatttg cacttccatg cccatggcct gcggcactac caggatgtcg gagaagatga
     1381 tcgccgcgtc cacacctacc tgttctaccg gttgaatggt gatctccgcc gccagttccg
     1441 gagtctggca tcgctcgaag aacgaatatt tttccttcag cttcagatag tccgggaggt
     1501 aacgccctgc ctgccgcatc atccataccg gcgtgcgttc ggtcttttct cctttcaggg
     1561 tccgtaacaa caggtcattc ttcaagatag cgcgttgtat caatgctgga tgtttttgat
     1621 gatttgatgg atcaatgtct cctcgtccgg tctttcgctc tccgtaaccg ggttacggca
     1681 tattcggcgt atagccccgg ccgttgtccc gccaatggca aagagcggaa tgtccggagg
     1741 aagggtattg accgagaaaa agctttccac ggcgcttggg ctaaagaaag caatcgcgtc
     1801 atagttgcgc ccgatccggt ggggcgtgag cctcgtgcgg tatacgaaga tctcgtggac
     1861 cttgagcccc gcctgcccga gcaagtccgg taactcatcc cggcgccggt cgccgcagaa
     1921 gaaccagagt tcgcgatcgg cccccgcgtc tatcccggaa gatgcctttt ccgccggcat
     1981 ttgccggacg atctcatccg ccagctccct ggccgaatcg gccgtccccg cgatggccgc
     2041 ccggccaaaa tattccgccg ccttctgcca tgtggcgccg cccagacaaa agatcttcca
     2101 cgcgcgacca ccacctcctt tcagtgccgt catcgcattg ttttccagtg ccgctagcgc
     2161 attgacgctg gtaaagacgg ccgtagacgg tcctgccggc agctctcccg ctcccagctt
     2221 cagcggctcg accgcaataa agggcaggat atcgatcctc accccgtttg ctgctgccgt
     2281 atccaccagg gtaggggaca agggccgggt ggacaacaca tatgctggag agctcggcat
     2341 gtcagtctaa gatgttttgc cggcaagggt ttctaccgcg cgaatgcctt ccactatgac
     2401 cc
//
LOCUS       DATGKE010000668         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_435795, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000668 DATGKE010000000
VERSION     DATGKE010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435795"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1228
                     /locus_tag="VKU83_11325"
     CDS             47..1228
                     /locus_tag="VKU83_11325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLI94197.1"
                     /translation="MGNTISRRALLQRGALLAGGLPFAGSLLNPMQAAPSVNGHALTA
                     TSSEEWSEKDIALASVEPLKARLFANENPFGPSDKAKKAIVETLPIAYQYPFTSIGEM
                     TAKIAAFEKISERNILMAAGSTPLLMAAALAYYRPGCNVVSGDPTYADLPSRAARIGA
                     KWVKVPVTADHKLDLGAMEKAVDGNTVIVYICNPNNPTATVVDNEELRGFVDRVSKRT
                     VVFIDEAYVDYLPDPQGATLIRMANSNPNIIVARTFSKLYGFAGLRLGYVVTQPDAIG
                     KLTAYTEAAFSISTTTIHAAIATFQDRESLDAALQKTVASKTYLYGVLEKAGYEYIPS
                     VANFVLFPIRMDGKKFVDEMMNRSVAVRFWKFNNQDWCRVSIGRMDEMEAFAAAFKDI
                     A"
     gene            complement(1267..>2092)
                     /locus_tag="VKU83_11330"
     CDS             complement(1267..>2092)
                     /locus_tag="VKU83_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94198.1"
                     /translation="MGYSYGYNLFTGFAEVISGVLLLFRRTTAIGAFMAFIVSANIVA
                     MNYSFDIPVKLLSSTMILMSLFLLGENVRRLWGLFFQNRTTRLHTVPRPRFRKKAPRI
                     ALAVVKYLLIAYVIVGYAISYLEARTQYGDRAPHVALYGIYNTRSFVLNGETLAPLQT
                     DSVRWKQLIIDGSPTMSFAGIKMMNDSMRRYRVATDSLLHLVTITDFADSANNWKLNY
                     LAGADSLILWGKHFGSKHFGRKDPDSIRVTLIRYPLEKFRLNSRGFNWINEFPNNR"
BASE COUNT          485 a          544 c          615 g          448 t
ORIGIN      
        1 tattgatccc ccagtaaacc gttttctaac ctcaaaacat ccgcttatgg gcaacacaat
       61 tagtcggcgc gccttgctgc aaaggggcgc gttgctcgct ggcgggctcc cttttgccgg
      121 cagcctgctg aatccaatgc aggccgcgcc gtcggtgaac ggacatgccc tgacggccac
      181 cagctcggaa gaatggtcgg aaaaagacat cgccctggct tccgtcgagc ctttgaaggc
      241 caggcttttt gccaacgaaa atccgtttgg accgtctgac aaggccaaga aagcgatcgt
      301 ggaaacgctg ccgatcgcct atcaataccc tttcacttcg atcggcgaga tgacggcgaa
      361 aattgccgca tttgaaaaga taagtgaaag aaatatcctg atggcggccg ggtccacgcc
      421 attgctgatg gcggcggcgc tggcttatta caggcccggc tgcaatgtgg tatcaggcga
      481 cccgacttat gcggacctac ccagcagggc agcacggatc ggcgcgaaat gggtgaaggt
      541 gccggtaacg gctgaccaca agctggacct cggagccatg gaaaaggcgg tggacggcaa
      601 taccgttatc gtatatatct gcaatcccaa caatcccacg gcaacggtcg tcgacaatga
      661 ggaactgcgc ggttttgtcg acagggtgtc gaagcggacc gtggtcttca ttgatgaagc
      721 ctacgtcgac tatcttcccg atccgcaggg tgcgacgctg atccggatgg caaattccaa
      781 cccgaatatc atcgttgccc gcaccttttc caaactttat ggttttgccg ggctgagact
      841 cgggtatgtg gtgacccagc cggacgccat cgggaagctg acggcatata ccgaagctgc
      901 gttttccatt tccactacta ccatccatgc ggccatcgct acttttcagg atcgggagtc
      961 gcttgatgcg gccttacaga agacggtggc ttcaaagacc tacctctacg gcgtgctgga
     1021 gaaggcgggc tacgagtata tcccgtcagt ggcgaacttt gttcttttcc ctatccggat
     1081 ggatggcaag aaattcgtgg atgagatgat gaaccgaagc gtagcggtgc ggttctggaa
     1141 attcaacaac caggactggt gccgggtcag tatcggccgg atggatgaga tggaagcgtt
     1201 tgcggccgca ttcaaagaca ttgcctagtc cttattcctg ccggcaaggt ggggagtccc
     1261 atcgcattat cggttatttg ggaattcgtt gatccagttg aatccccggg aattcaagcg
     1321 gaatttttcc agtggatacc ggatgagcgt aacccggatc gaatcggggt cttttctgcc
     1381 gaagtgcttg ctgccgaagt gcttgcccca taagatgagg ctgtcggcgc ccgccaggta
     1441 gttcaatttc cagttgttgg cggaatcggc aaagtcggtg atcgtgacaa gatgaagcag
     1501 gctgtcggtc gcaacccgat accggcgcat gctgtcattc atcattttga ttccggcaaa
     1561 ggacatggtc ggcgaaccat cgatgatgag ttgtttccag cggaccgagt cggtctgaag
     1621 cggcgccagg gtctccccgt tcaacacgaa ggaccgggtg ttgtaaatgc cgtataacgc
     1681 gacatgaggg gctcgatcgc catactgggt ccgggcttcc aaatagctga tggcatagcc
     1741 gacgatgacg taggcgatca gcaggtattt aacgacggca agcgcgatcc gcggggcttt
     1801 tttgcggaat cgcggccggg gaacggtatg gagacgggtc gtccggttct ggaagaagag
     1861 accccacaac cgccgcacat tctccccgag caggaacagg gacatcagga tcattgtgct
     1921 ggagagcagc tttacgggga tgtcgaaaga gtagttcatc gccacgatat tggccgatac
     1981 gataaacgcc ataaaagctc cgatggcggt cgtgcggcga aacagcagca gaacgccgga
     2041 gatcacttcg gcaaaaccgg tgaaaagatt atatccataa gagtagccca tg
//
LOCUS       DATGKE010000669         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_641480, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000669 DATGKE010000000
VERSION     DATGKE010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641480"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1142
                     /locus_tag="VKU83_11335"
     CDS             408..1142
                     /locus_tag="VKU83_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLI94199.1"
                     /translation="MKKLIVCILCLASTQVFAQFRLGIQASFSSLNCWQSDQIGGLPT
                     GAQTRQMNGFEAGLVAEYDLGYSGFIIQPALLYAENGTNFGNTQGFIDNADYSIGYSS
                     SYIRFYSLRLPINLVYKYAINDKWRAFVGIGPYVSKSLSGTEKGYYQVVYTANNQFAA
                     FPINNKMNISNNPSYAPGGVSNMAGFDFGADFLVGVSYKKFDFSASFNRGFTEMYHTT
                     YTDLGNQFWNFTVAYTILGHYRKPKP"
     gene            complement(1161..1619)
                     /locus_tag="VKU83_11340"
     CDS             complement(1161..1619)
                     /locus_tag="VKU83_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94200.1"
                     /translation="MKSILFALLLSTGAAVISEAQGYVNPTGTYLLKGDVRNNEIIGH
                     YGELRVRLLDSSRIALAFYLNNGYPGYESGSFVDTLPYDDNRAVYRPGTDSSCRIVFS
                     FGIRSAEVSQVVSNPGSGCGFRPGVIEPALLHKTSSGIPVIQDLSLHGSR"
     gene            complement(1728..2627)
                     /locus_tag="VKU83_11345"
     CDS             complement(1728..2627)
                     /locus_tag="VKU83_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLI94201.1"
                     /translation="MIIRIGRILLRVAASLVIFLIVLLLIADRFIEFRMDNKDLTQWF
                     HSRNIQPEISYYEAGGRKIRYIAIGDNPEATILFIHGAPSSMSYWRGYLADSILRSRA
                     TMYAVDRPGYGYSGLGDPLPDIAAQAADISKILDSLHHANHPVIVVGVSYGAPIACRL
                     TMDYPQLVDGLIVMAPPLGPGMERYFWFTHPVESPLVRWVVPRMLRSANREKVHHKEE
                     LEKMLPLWSRIHVPVMYLQGLDDGLVDTGNASFARTHLTNVPYLDIQMIPGRGHLIAF
                     EEKDRIEKAILKMIDLCEKRQLR"
     gene            2814..>3275
                     /locus_tag="VKU83_11350"
     CDS             2814..>3275
                     /locus_tag="VKU83_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94202.1"
                     /translation="MFKHYLKVAWRNLLKDRQFSLLNLLGLSVGLACALLIGLWVLDE
                     MNMEKYNPNDNRLYQVMTNNKTSNGLQTGMYTPGILAKSLRDEFPEIQDASEVLPASW
                     FNDPNTPSGVISYKDKKLNATPQLVDSNFFHLFNCPVLEGDRRRLFADKQGV"
BASE COUNT          805 a          875 c          801 g          794 t
ORIGIN      
        1 tctacaagtc aattagcgcc ggcgcgcggc cgcgcgctac ggatcctggc acggttttat
       61 atcttgtatg atcaaaggat ggtgatttcc cgtttaacat ttacaggcgg tgtaaaagct
      121 agcatgtaaa aaaataaaag gaataagcat ccgcgaaagc tgtgttccat tgggagtaca
      181 gctttcttct tttagtgggt gacagcggtt accagccgaa ggccaggcga tctccacgcc
      241 tcataccttt tcttatcaaa ttagtgtttt tgatctcaat tcaattattt ttgcccgttt
      301 tccgggccgc cgcggggccg ccgcggggtt atgggaatgt taaaacgtag ccgaattgca
      361 gcaaaagtta tagtttgccg gtcctaagag taactcaata gactagtatg aaaaaattga
      421 ttgtctgtat tttatgtttg gcgtcgacac aggtttttgc acaatttcga ctgggcatcc
      481 aagcctcctt cagttcgctt aactgctggc agagcgacca gatcggcgga ttgcccaccg
      541 gcgcgcagac gagacaaatg aacgggtttg aggccgggct tgtagcggaa tacgacctcg
      601 gttattcggg ttttatcatt caacccgccc tgctttacgc tgagaacgga accaattttg
      661 gcaataccca gggctttatc gataacgctg actattccat tggctattcc agcagctata
      721 tccgtttcta ctctctccgc cttcccatta acctggttta taagtatgct attaatgata
      781 aatggcgggc ctttgtgggc attggcccct atgtgtcgaa gagcctgagc ggcacagaaa
      841 aaggatatta ccaggtcgtg tacacggcaa ataaccagtt cgcggcgttc ccgatcaata
      901 acaaaatgaa catttccaat aatccctcgt acgcgcccgg cggcgtctcc aatatggcag
      961 gcttcgactt cggggcggat ttcctggtgg gggtctccta taagaagttt gatttctcgg
     1021 ccagtttcaa ccgcgggttc accgagatgt accacaccac ttacacggac ctgggaaatc
     1081 aattctggaa tttcacggtg gcctacacga tccttggcca ctaccggaag ccgaagccgt
     1141 agccgcacca tccagaccgc ttagcggctt ccatgaagcg acagatcctg aataacagga
     1201 atgccggacg aagtcttatg caacaacgcc ggttcgatca ctcccggccg gaagccgcag
     1261 cctgaaccgg gattgctgac tacctgggag acctcggccg accggatccc gaatgagaaa
     1321 acgatgcggc atgaggagtc tgttcccggc cggtacaccg cccggttatc atcatacggc
     1381 aatgtgtcca cgaatgaccc cgattcatac ccgggatatc cgttatttaa atagaatgca
     1441 agggcgatcc tggagctgtc gagaagccgg accctcaatt ccccgtaatg gccgatgatc
     1501 tcgttattcc gtacatcgcc cttaagaaga taggtgcctg tcgggttcac gtaaccctgg
     1561 gcttcgctga ttacggcggc tccagtggag agtaagaggg cgaaaaggat gctcttcata
     1621 ccaactgatt tggcgtaatt gaagaagctt tctcatgaac ttgcgcttcc taatttacga
     1681 aaaaaaaata cttccggtaa aatttggtat gataaaaaaa atattagtca tcgcagctgc
     1741 cgcttttcgc agagatcgat cattttcaaa atggctttct cgatcctgtc cttctcttcg
     1801 aatgcgatca ggtgtcccct tcccggtatc atttgaatat cgaggtaggg gacattcgtt
     1861 aaatgcgtcc tggcgaaact tgcattgcct gtatcgacca acccatcgtc gaggccctgc
     1921 aaatacatga ccggcacgtg gatccgcgac caaagcggca gcattttctc caactcttcc
     1981 ttatgatgca ccttttcccg gttggctgac cgaagcatgc ggggcaccac ccagcgcacc
     2041 aggggacttt ccaccggatg cgtaaaccaa aaataccgct ccatgccggg acccagaggc
     2101 ggcgccatca cgatcaatcc atccaccagc tgcgggtaat ccatcgttag ccggcaggcg
     2161 atgggcgcac catagctgac gcccaccaca atgaccgggt gattggcgtg atgaaggcta
     2221 tcgaggatct tactgatatc cgccgcctgt gcggcgatat ccggcaacgg gtcacccaat
     2281 ccggaatacc cgtaaccggg ccggtcgacc gcatacattg tagcgcgcga ccgcaggatg
     2341 ctgtctgcta aataaccccg ccaataactc attgaactcg gcgcgccatg tatgaagaga
     2401 atggtcgcct ccggattgtc acctatggca atataccgga tctttcggcc gccggcctca
     2461 tagtagctga tctcgggctg gatattgcgg gaatggaacc actgggtcaa gtccttgtta
     2521 tccattctga attctataaa ccggtcggcg atcagcagca aaacaatcag gaagatcacc
     2581 aaactcgctg ccactctgag caaaatccgg ccgattctaa tgatcataca tcgaaaatag
     2641 caattattac gccccacaac aaagggtcgc tgctcgccag cgcctgactt cacgcggaca
     2701 ctggctgtat cgttttcgaa cacattgggc tcaggtcaga aatataatac cctgatgact
     2761 aattttttga aattccggag acgtttttgt tccggtttgc caaactgctc tccatgttca
     2821 agcattatct gaaggtcgcc tggcgcaact tgctgaagga tcgccaattt tccctcctca
     2881 atttgctcgg gctctccgtt ggattggcct gtgccctcct tatcgggctt tgggtcctcg
     2941 acgaaatgaa catggagaaa tacaatccga atgacaaccg gctttaccag gtgatgacca
     3001 acaacaagac gtccaatggt ctgcagacag gtatgtatac gccgggtatc ctggcgaaat
     3061 cattgagaga cgaatttccg gagatccagg acgcttccga ggttttaccc gcctcctggt
     3121 tcaatgaccc gaatactcca agcggggtga tctcttataa ggataaaaaa ttgaacgcta
     3181 cgcctcaact tgtcgacagc aatttctttc acttattcaa ctgcccggtc ctagaaggag
     3241 accggcgccg cctgttcgcc gacaagcagg gcgtg
//
LOCUS       DATGKE010000670         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1257294, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000670 DATGKE010000000
VERSION     DATGKE010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257294"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1416)
                     /gene="rseP"
                     /locus_tag="VKU83_11355"
     CDS             complement(58..1416)
                     /gene="rseP"
                     /locus_tag="VKU83_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLI94203.1"
                     /translation="MTLLAIDWSSVGIKVVQFILSFSILVILHELGHFLPARWFKCRV
                     EKFYLFFNPWFSVFKKKIGETEYGLGWVPFGGYVKISGMIDESMDKEQLAQPPQPWEF
                     RSKPAWQRLIIMLGGVTVNVLLAFTIFIVIMWVWGDRYIPVKNLKYGLYADTLGRKIG
                     LQDGDNIIAVGGSPVTRTTVIPTQIIINEATSLTILRDGKTMKLAIPPGFISQLSRNR
                     FEGFFGIQYPQVVDSVPPDVKVLKGTVERGDTLIGVDGRYIRYASDLYRDTSFNYLTR
                     AKQQVEIYKRGNDTVSALLARKPKGGTGIHFKNPEDILGVVQINYTLLQAIPAGIHKS
                     MTTLSNYIRNLRLLFTSKELKVQDNLGSVVSIASVFPSEWDWESFWSLTAIFSILLAF
                     MNVLPIPALDGGHALFTLFEMISGRKPSDKFMEYSQLAGMVLLFGIMAYAMGLDIFRF
                     FR"
     gene            complement(1453..>2104)
                     /locus_tag="VKU83_11360"
     CDS             complement(1453..>2104)
                     /locus_tag="VKU83_11360"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLI94204.1"
                     /translation="KVILTASGGPFLGKKPNYLVNVKRDHALQHPNWNMGAKVTIDSA
                     TLMNKGLEMIEAKWLFGLKPEQVRVVIHPQSIIHSMVQFEDGSIKSQMGLPDMKLPIQ
                     YALSFPQRLGSNFPRYDFRKPANLTFEEPDTKTFRNLSLATEALHKGGNLPCVLNAAN
                     EIAVFAFLRNRIGFLDMTDLVERTMQEMPFIGRPSLEEYFESDGEARNFAASLIKL"
BASE COUNT          485 a          609 c          528 g          482 t
ORIGIN      
        1 tacgcctctc gacagcaagc tgccggcttt cccataaccg gagcagttgc ctattgccta
       61 ccggaagaac ctgaatatat ccaaccccat ggcgtaggcc atgatgccga acaggaggac
      121 catccctgcc agttgcgaat actccatgaa cttgtcgctt ggcttccgcc cgctgatcat
      181 ctcgaacagg gtaaataagg catgtccgcc atccagggcg gggatcggca atacattcat
      241 gaacgcgaga aggatgctga agatcgccgt cagcgaccag aagctctccc aatcccattc
      301 cgagggaaag acactcgcaa tgctgactac gcttcccaga ttatcctgca cctttagctc
      361 cttggaagtg aacagcaacc gcagattgcg gatatagttg cttagcgtcg tcatgctctt
      421 atggatcccg gcgggtatcg cctgcagcaa agtgtaattg atctgcacca ccccaaggat
      481 gtcttcgggg tttttgaaat ggatcccggt gccccctttc ggctttcgcg ccagaagcgc
      541 gctgaccgtg tcgttgccgc gtttgtagat ctctacctgc tgctttgcac gggtcaggta
      601 attgaaggaa gtatcgcggt aaaggtccga ggcataccgg atataacgcc catccacccc
      661 gatcagcgta tcccctcttt ccaccgtgcc ctttagcacc ttcacgtcgg gcgggaccga
      721 atcgacgacc tgcggatact ggatgccgaa aaagccttca aaccggttgc ggctcagctg
      781 cgagatgaaa cccggcggga tggcgagctt cattgtcttt ccatcccgga ggatcgtcag
      841 gctcgtggct tcattgataa tgatctgggt cgggatcacc gtcgtccggg taacgggtga
      901 accccccacg gcgatgatat tgtccccgtc ctgcagcccg atcttccggc caagcgtgtc
      961 cgcgtacagt ccatatttga ggttcttcac cgggatatac cggtcgcccc acacccacat
     1021 gatcacaatg aatatggtga atgccagcag cacattgacg gttaccccgc caagcataat
     1081 aataagccgc tgccatgccg gcttgctgcg aaactcccag ggctgcggcg gctgggccaa
     1141 ttgctcctta tccatgctct cgtcgatcat ccctgaaatt ttcacgtagc cgccgaaggg
     1201 cacccaaccc aggccgtatt ccgtttcccc gatcttcttt ttaaagaccg agaaccatgg
     1261 gttgaagaag agataaaact tttctacccg gcatttgaac caccttgccg gcaaaaaatg
     1321 ccccagctca tggaggatca ccagtatgga aaacgataaa atgaattgga cgactttgat
     1381 tccaacagaa gaccaatcga ttgctaataa tgtcataatt tctttcgtta cgcgtattct
     1441 tcgatacgcc cgctacagct tgatcaggga cgccgcaaaa ttacgggcct ccccatcgct
     1501 ttcaaaatat tcctcgaggc tcggccggcc aatgaacggc atctcctgca tcgtgcgctc
     1561 aaccaggtcc gtcatatcaa ggaagccgat ccggttccgg agaaaggcaa aaacggcgat
     1621 ctcattcgcc gcattgagta cgcaggggag attaccgccc ttatgaagcg cttcggtcgc
     1681 cagggacaaa ttacggaaag tcttggtatc gggctcttcg aaagtcagat tagccggttt
     1741 ccggaagtca tagcggggaa aattgctccc taaacgctgc gggaagctca gtgcgtattg
     1801 gatcggcagt ttcatatccg gcaaacccat ctgcgacttg atgctcccgt cctcgaactg
     1861 gaccatggag tgaataatgc tctgagggtg gataacgacg cggacctgct ccggcttcag
     1921 gccgaacagc catttcgcct cgatcatctc caatccttta ttcatcagtg tagccgagtc
     1981 gatagtgacc tttgcgccca tattccagtt gggatgctgt agcgcgtggt cgcgcttcac
     2041 gttcacgaga taattcggct tcttgcccaa aaacggtccg cccgatgcgg tcagaatgac
     2101 cttt
//
LOCUS       DATGKE010000671         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1495005, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000671 DATGKE010000000
VERSION     DATGKE010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495005"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..551
                     /locus_tag="VKU83_11365"
     CDS             51..551
                     /locus_tag="VKU83_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLI94205.1"
                     /translation="MEKNGQIEKPPTVPVELDEKDKAILRLLQDNAKITVREIAAQVH
                     LSTTPVHERIRRLEETGVIRQYATLLDHSKVKKGLLAICYVSLKEHNKKSGARFIRTI
                     QMMPEVMECFIISGEFDFLLKVAVENMDAYYDFHVNHLGQIENVGHVQSTFVMGVVKQ
                     SHQLIS"
     gene            589..3006
                     /locus_tag="VKU83_11370"
     CDS             589..3006
                     /locus_tag="VKU83_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94206.1"
                     /translation="MIGHYLKIAWRSLRKDGFFSAINVIGLAIGLAVSLQIGVYLNHE
                     WSYDAYNLQADRIFRMATDIHISGTSIRSVNTPSPFAAGMVRDFPAIQSAVRIRHIPE
                     VLVIAAGKSFSDNGVFRADPTLFDIFTIPMLAGDPRTALVEPNSAVIAESTAKTDFGG
                     TDVIGQTFRTNDYDSVVYRITGVFKDLPPESHFHFHLIESASGRKEEFRYWAGLWGAT
                     YLLARPGTNTEAINRMLSTETNRYVDRALRQTMQDGLSDLAKRGDYWRFYAMPLKDIH
                     LHSHLLAEFEANGDSQRMGIFLLIAILILLIAGINFVNLSTARAARRLREVGVRKILG
                     SYRRQLVVRFLAESMLMGGAATFLGYFMAIGLLPVFDHLTGSNFTPAIFFHVGWLAAL
                     VPAAAGIGLLAGIYPAHYLSAFRPINLLKGILKTRTGSRSMRNCLVVFQFSAAILLIC
                     ATGVIYSQLKFIQHSDLGYNREQVLTIHGTNYMGDGAAIFQQQVEQLPGIVSGTMTGF
                     LPNTLHPGTRGFLRDGIGRTTGTVLMGDWRIDTGYVSTLGLTILSGRNFRSNLATDAG
                     GVLINEAAARALGYRDPVGKNVYTLGADSAHPVLGVLRDFKVASMHESIQPIVFELSR
                     DSSAISFRVHTTNLSGLISRIRDCYDALRGPAAQPFSYTFLDDDFNKLYAEDERTGQM
                     ILVLTVIALLIACLGVLGLVTSAAERRGKEIGIRKVLGAGSRQVMLLLSRDFLRWILL
                     AFLIACPLAWWFMQQWLQGFAYRTAMHWWIFLFAGSSCLSIAALTISWQVIRAATVNP
                     VERLRME"
BASE COUNT          667 a          808 c          866 g          757 t
ORIGIN      
        1 aataagtggc tacatacaag catttctata gaacaaaaat ctaataactc atggaaaaga
       61 acggccaaat agaaaaaccg cctaccgtcc ccgtcgagct cgacgaaaag gacaaggcca
      121 ttttgcgcct gctgcaggat aatgccaaga tcactgtgcg ggaaatcgcg gcccaggtcc
      181 atctcagcac gacgccggtc catgagcgga tccggcgcct ggaggagacg ggggtgatcc
      241 gccaatatgc caccctgctg gatcattcaa aagtgaagaa gggtttgctc gccatctgct
      301 atgtgtccct caaggaacac aataagaaat ccggcgcgcg gtttatcagg acgatccaaa
      361 tgatgcccga ggtcatggag tgtttcatca tctcgggcga gttcgacttc ctgctgaagg
      421 tggcggtcga gaacatggat gcgtactatg attttcatgt gaatcacctg ggccagatcg
      481 aaaatgtggg tcatgtgcag agcacctttg tcatgggggt cgtcaagcaa agtcaccagc
      541 tgatttcgtg atttatggaa aaaggccgcg ttcgcatctc agactctgat gatcggtcac
      601 tatctgaaga tagcctggcg cagcctccgg aaagacggtt tcttttcagc cattaacgtc
      661 attggtctgg cgattgggct ggcggtcagt ctgcaaatcg gcgtgtatct gaaccacgaa
      721 tggagctacg atgcttataa tctccaggcg gaccggatct tcaggatggc cacggacatt
      781 cacatcagcg gcaccagcat ccgcagcgtc aacacccctt ctccgttcgc cgcaggaatg
      841 gtacgggact ttccggccat ccaaagcgcc gtccgaatcc ggcacattcc tgaggttttg
      901 gtgatagccg ctggcaaatc attctctgat aatggtgtgt tccgcgctga cccgaccttg
      961 ttcgatatct ttaccatccc gatgctggcc ggcgacccgc gcacggctct cgtagaaccc
     1021 aattccgcgg tgatcgccga aagtactgcc aaaacggatt ttgggggcac ggacgtcatc
     1081 ggccagacct ttcgaacaaa tgactacgac tcggttgtat atcggatcac cggcgtcttc
     1141 aaagacctcc cgccggaatc gcacttccat tttcatttaa tcgaatcggc gtcgggacgg
     1201 aaagaggaat tccgatattg ggccggactc tggggtgcca cctacttact cgcccggccg
     1261 ggcacaaaca cggaagctat caaccggatg ttgtcgactg agacgaatag gtatgtggat
     1321 cgtgcgcttc ggcagacaat gcaagatggc ctgtctgacc tggcaaagcg cggcgactat
     1381 tggcggtttt atgcaatgcc cctgaaggac attcatttgc actctcattt gctcgccgaa
     1441 tttgaggcaa acggagacag tcagaggatg ggcattttcc tgctcattgc tattctcatt
     1501 ctgctcattg cgggcatcaa ctttgtcaat ctgtctactg cccgtgccgc gcgtcgcttg
     1561 agggaggtgg gtgttcgaaa gatattgggt tcttaccggc gacaattggt cgttcggttc
     1621 ctggcggaat cgatgttgat gggcggtgcg gcaacattcc tgggctattt catggcaatc
     1681 ggtttgttgc cggtttttga tcatctaacc ggttcgaact ttactcctgc gatctttttc
     1741 catgtaggat ggcttgctgc cctggtcccg gccgcagcag gaattggcct gctggcaggg
     1801 atctatcctg cccattactt gtctgccttc cggccaatca acctgttgaa ggggatactt
     1861 aaaaccagga ctggcagccg ttcgatgcgg aactgccttg tcgttttcca gttcagtgcg
     1921 gctattttgc tcatttgcgc aaccggtgta atttatagtc agctgaaatt tattcagcat
     1981 tcggatctgg gttataaccg ggagcaagtg ctgacgattc atggtacgaa ttacatgggt
     2041 gacggggctg cgatctttca gcagcaggta gagcaactcc ccgggatagt aagcggaacg
     2101 atgacgggtt tcctgccgaa cacgctgcat cccggaaccc gcgggtttct cagggatggc
     2161 atcggccgca cgaccggcac agtccttatg ggtgactggc ggatcgatac cggttatgtt
     2221 tcgacgctgg gcttgacgat tctgtccggg cgcaacttca ggtctaattt agccactgat
     2281 gcaggcggtg tgctgatcaa cgaagcggcg gccagggcgc ttggatatcg cgatcccgtc
     2341 ggaaaaaacg tctatacgct cggcgccgat tcagcacatc ctgtcctggg tgttctcagg
     2401 gacttcaaag tcgcgagcat gcatgagtcg atccagccga tcgtctttga attgagcaga
     2461 gactcatccg ccatatcatt tcgggtccat acgaccaacc tgagtggcct catttcccgt
     2521 attcgcgatt gctatgacgc attgcggggc ccggcggccc agccattctc ctataccttt
     2581 ctcgatgacg acttcaataa gttgtatgcc gaagatgaac gtaccggcca aatgatattg
     2641 gtgttgacgg tcatagcatt gctgatcgcg tgcctgggcg ttctcggatt ggtcacctcc
     2701 gctgcagaac gacgggggaa ggagatcggt atccggaaag tcctgggagc cggcagccgg
     2761 caggttatgc tgttattgtc gagggacttc cttcggtgga tattgctggc ctttcttatc
     2821 gcgtgcccac tggcttggtg gttcatgcag cagtggctgc agggatttgc ctaccggacc
     2881 gccatgcatt ggtggatctt tcttttcgcc ggatcaagct gcctgagcat agcggcgctg
     2941 acaatcagtt ggcaggtcat cagggcagcg accgtcaatc ctgtcgaacg gcttcggatg
     3001 gaataggcgc cggcaggcaa gcatccgtga cgggctggcg gcatacagct ggtatttcgt
     3061 aactttccgg tgtcaaaatc gtcgtatatg ttcaccta
//
LOCUS       DATGKE010000672         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_673257, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000672 DATGKE010000000
VERSION     DATGKE010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673257"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VKU83_11375"
     CDS             complement(<1..1579)
                     /locus_tag="VKU83_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94207.1"
                     /translation="MNAYSPHRKRGRGAAIAILLILSGTCHDRALAQTDSTGPRKPDP
                     MTWKDIPAWKYINPQKVELSPDGKWLAWPLLTTEGDGELVIRNTNDTITRKYPIGGNN
                     QPSFEFSDDGQWIAIKQSSSYKEIRAAARTPGKQVFDKLLLVNLATGQMTSFEKAGGY
                     AFNGKAATCVAISLEREKQPNAKPDDPKNSELLLIELKTGKIQDIGDVGEFAFNKPGT
                     FLAYTTETPGKMGNGLYLLTVGTRQTIVLDNDKMDYKFLRWKDEGDALAVLKTKKDTG
                     WKTPRGVVIGVKEPGPSPVLVSYDPAKDSTSFPKGMTISSNRAPVWSDDLSRLFFGIA
                     SLRPDKKPTSADSLARKNAGDDTVKPDMIIWNGKDPRLQSRQQIEETQDREFSWYGMV
                     EVANRHFTRLNDSTQRKLTLLPKHHYALIEDNASYELDQNLDGQNYEDLYIVDLATGR
                     SRKWMDKFYLPSFQSAPRPSPDGTKLVYGLDGQYYVYDIPADRSVNITEEVPASFVNT
                     EDDHNVTKPLTAVIGWSS"
     gene            complement(1622..2578)
                     /locus_tag="VKU83_11380"
     CDS             complement(1622..2578)
                     /locus_tag="VKU83_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLI94208.1"
                     /translation="MPSRRKFLQQLGTVTTSAVLLPQLGQAAGIFSSRSTKPVGLQLY
                     TLGELMVTDPKGTLQKVAAIGYKEVESAGSQKGNFYGFTPKEFSAMVKDAGLHWRSAH
                     IGGAPFSVEQIMKMAKNAADSARIQRMMERFKNRPKTENWKDDYQKLADEGAEGGLSY
                     MVCSSIPVSTMDEIKTAVDVFSKAGEACKKNGIQFAYHNHVTEFDEVEGHRPFDYILN
                     NTDKDLVKMELDLAWATKAKQDPVQLFKLHPGRYPLWHVKDLDKTTMSPAEVGSGVVD
                     FKRIFANSKESGMKYFFVEQDGAPQPLQNIANSYNYLEKILL"
     gene            complement(2621..>3008)
                     /locus_tag="VKU83_11385"
     CDS             complement(2621..>3008)
                     /locus_tag="VKU83_11385"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLI94209.1"
                     /translation="LNNMKRVTGIGGIFFKCQDPEKIKEWYKTHLGLDIDRYGAKFDW
                     RDVADSAKKGSTVWSPFSEKTRYFEPSSKDFMINYRVENLEALVGELKKEGVTVLDNM
                     EAYDYGKFVHILDIEGNKIELWEPKE"
BASE COUNT          596 a          811 c          766 g          835 t
ORIGIN      
        1 cgctgctcca gccaatgaca gccgttagcg gcttggtcac attgtggtcg tcttcggtgt
       61 tgacaaaaga cgccgggacc tcttccgtaa tgttgaccga gcggtcagcc ggaatatcat
      121 agacgtagta ttgaccgtcc agaccataga ccagcttggt tccgtccggt gaagggcgtg
      181 gggcagattg gaaagaaggg agataaaatt tgtccatcca ctttctcgac cgccccgtcg
      241 cgaggtccac gatatacaga tcttcataat tctgcccgtc aagattttgg tcgagttcat
      301 aggatgcgtt gtcctctatc agtgcataat gatgtttcgg gaggagggta agcttccgtt
      361 gcgtactgtc atttagccgg gtaaaatggc ggttcgcgac ctccaccatc ccataccacg
      421 aaaactcgcg gtcctgcgtt tcttcgatct gctggcgact ttgcagccgg gggtccttac
      481 cgttccaaat gatcatgtcc ggcttgacgg tatcgtcgcc ggcgttcttt ctggccagac
      541 tatccgctga ggtgggtttc ttgtccggcc tcaggctcgc aattccaaaa aacagccgtg
      601 aaaggtcatc agaccaaacc ggggcccggt tactgctgat cgtcattccc tttggaaacg
      661 aggttgaatc ttttgccgga tcgtagctga ccaataccgg cgacgggccg ggttccttaa
      721 cgccgatcac cacgcccctg ggcgttttcc acccggtgtc ctttttcgtt tttaggaccg
      781 ccaacgcatc cccttcgtcc ttccagcgga ggaatttgta gtccattttg tcgttgtcga
      841 ggacgatagt ctggcgtgtg cccacagtga gcaggtacag gccattcccc atcttgcccg
      901 gcgtttcggt ggtgtaggcg aggaaagttc ccggcttatt aaacgcaaat tcgccgacgt
      961 ctccgatgtc ctggatcttc ccggtcttta gctcgatgag cagcagttcg ctgttcttcg
     1021 ggtcgtccgg tttcgcgttg ggctgctttt cgcgctcgag ggaaatggcg acacaggtcg
     1081 ccgcctttcc gttgaaggca tacccaccgg ccttttcaaa gctggtcatt tggccggtgg
     1141 ccaggtttac gagcagcagc ttatcgaaga cctgtttgcc cggggtcctg gcggccgcac
     1201 ggatctcctt atacgatgac gactgtttga ttgcaatcca ttgtccgtca tctgaaaatt
     1261 cgaagctggg ctgattgttg ccgccgatgg gatatttccg ggtaatcgta tcgtttgtat
     1321 tcctgatgac cagttccccg tcgccctcgg tggtcagcag cggccaggcc agccatttgc
     1381 cgtccggtga aagttcgact ttctgggggt tgatgtattt ccacgccgga atgtccttcc
     1441 aggtcatcgg gtccggtttg cggggtcctg ttgagtccgt ttgggccaat gccctatcgt
     1501 ggcacgtgcc agacaatatg agcagaatag cgatggccgc tccgcggccg cgcttgcggt
     1561 ggggggaata ggcgttcatg cgctaaatat aacgattccc cgattatgaa tccggcgttc
     1621 gttaaagcag tatcttctcc aggtagttat aactgttagc aatgttttgc agcggctggg
     1681 gcgccccgtc ctgctcgaca aagaaatatt tcattcccga ttcctttgaa ttggcgaaaa
     1741 tgcgcttaaa atcaacgact cctgaaccga cctccgcagg ggacatcgtc gttttatcca
     1801 ggtccttaac atgccaaagc gggtaccgtc ccgggtgaag cttgaatagc tgcaccgggt
     1861 cctgttttgc cttcgttgcc caggccaggt cgagttccat ttttacgaga tccttatcgg
     1921 tgttattcag aatatagtcg aacggacgat gaccttccac ttcgtcaaat tccgttacgt
     1981 gattgtgata ggcgaattga atgccattct ttttacaagc ttcgcccgcc ttgctgaaaa
     2041 catcgacggc cgtctttatc tcatccatcg tgctcacggg aatactggaa caaaccatgt
     2101 agctgaggcc accttcagcg ccctcatcgg ccaatttttg gtagtcgtct ttccaatttt
     2161 cggttttagg ccggttcttg aatcgttcca tcattctttg tatcctggcg gagtccgccg
     2221 cattcttggc catcttcatg atctgctcga cggaaaacgg cgcgccacct atgtgagcgg
     2281 agcgccaatg cagacccgcg tccttgacca tcgctgaaaa ttcttttggc gtaaatccgt
     2341 agaaatttcc tttttgcgag ccggcgcttt ctacttcttt gtagccgatg gcggcgacct
     2401 tctgaagcgt acccttcgga tcggtgacca tcagttcacc aagcgtgtag agttgaagcc
     2461 cgactggctt ggtggaccgg ctggaaaaaa ttccggccgc ctgaccgagt tgtggcaata
     2521 aaacggctga agtcgtaacg gttcctaatt gctgaaggaa ttttcttctt gatggcatag
     2581 caaaaacgtt ttttgtgatt aagctcccga atatacgact ttactccttt ggctcccaca
     2641 attcgatttt gtttccttct atgtcaagaa tgtgaacgaa ctttccgtag tcatacgctt
     2701 ccatattgtc cagcaccgtt acgccttcct tttttagctc tcctaccaac gcttccagat
     2761 tttcgacccg gtaattgatc ataaagtcct ttgacgacgg ctcaaaatat ctcgtcttct
     2821 ccgaaaacgg gctccagacg gtagatccct tttttgccga gtcggccacg tcccgccagt
     2881 caaacttcgc tccgtaccgg tctatgtcca agccgagatg ggttttgtac cattctttga
     2941 ttttttccgg gtcctggcat ttgaagaaaa tgcccccgat tccagtaact cgtttcatgt
     3001 tgtttagg
//
LOCUS       DATGKE010000673         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_960907, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000673 DATGKE010000000
VERSION     DATGKE010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960907"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VKU83_11390"
     CDS             complement(<1..686)
                     /locus_tag="VKU83_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HLI94210.1"
                     /translation="MRTVCRTISLGWVLLLALASCTPGSRKLDRRVSLRHRDRIPYGT
                     KVAFDGLPFLFPNSTISYDDKPLSARASADGRTACIIVATVMDPSQSDINSILDFVGS
                     GNHVFISARVFGDSLLHALRLKAERDVYTYSLVMDSLRLSIRQPVTGDSLNFSYPGDS
                     YEGWITGYDPKYTSVLGRDRLGRPDFVRFNYKGGGTLYLQFAPLAFSNFFLLHKKNIA
                     YYEAALSYLPA"
     gene            complement(683..1480)
                     /locus_tag="VKU83_11395"
     CDS             complement(683..1480)
                     /locus_tag="VKU83_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HLI94211.1"
                     /translation="MRVRYRAALSTLVLGMFLCGRVIAAVQDTVRGDVAADSAVAPAV
                     GPARAAADSAASGERTAADSAERPALRRVADSTVTEWQAERAFAYANDPRYWRREDRN
                     EKRDSRLWTLLTGKWAGRILLFLLGAILLYAILRIIADNNLQLFYRSPKRQAAPIANH
                     EPSPIEDDLEGRLQQYLQAGDYRQAVRYLYLMSLRLLNERGLITYHQEATNREYWQQL
                     GETPQGPPFRDLTMIYEKVWYGEFPLGDPLFRRVQQFFEDFYKTVRA"
     gene            complement(1573..2427)
                     /locus_tag="VKU83_11400"
     CDS             complement(1573..2427)
                     /locus_tag="VKU83_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HLI94212.1"
                     /translation="MREGLFIKNNIDKWRQYQYQPANDPDEMAGQFTELVNDLGYAKT
                     FYPHSRVTQYLNGLSSRIYLGIYRNKKEESSRIIRFWKEELPQVVFRYRREILYSFLI
                     FVLFAIVAIFSAAHDESFVRGVLGDNYVDMTEDNISKGDPFGVYKQENPVNMFLYIVL
                     NNVQVAFMMFVAGFLLGIGTVWLLFQNGVMVGAFQYMFFAHGLGWKSVLVIWIHGTLE
                     ISSFIIAGAAGLVLGNSILFPGTHKRVHSLRRGARDGLKMLVGLVPVFTVAAFLEGFV
                     TRYSSMPL"
BASE COUNT          629 a          720 c          674 g          543 t
ORIGIN      
        1 gccggcaaat acgataatgc agcttcatag taggcgatat tttttttatg gagcaggaag
       61 aaattgctga atgccagcgg cgcgaattgt aggtacagtg tgccaccccc tttataattg
      121 aaccggacaa agtccggccg gccaagccgg tcccgtccaa gcacggaggt gtatttcgga
      181 tcgtagccgg ttatccagcc ctcataggaa tcgcccggat agctgaagtt cagcgaatcc
      241 ccggtaacag gctggcggat actcagccgg aggctgtcca tcacgagcga gtaagtgtaa
      301 acatccctct ccgccttcag gcgcagcgca tggagaagcg agtctccgaa taccctggct
      361 gaaatgaaga catgattgcc tgaacccacg aagtcgagga tcgaattgat atccgattgg
      421 ctcggatcca tcactgtggc aacaatgata caggccgtcc tgccgtcagc cgaggccctt
      481 gctgaaagcg gcttgtcgtc ataggagatc gttgaattgg gaaaaagaaa aggcaggccg
      541 tcaaaagcga ccttcgtgcc ataagggatc ctgtcgcggt ggcgcaagct gacgcgccgg
      601 tccagcttcc tggatcccgg cgtacaggaa gcgagcgcca gcaacagcac ccatccaagc
      661 gatatggtcc ggcaaaccgt cctcatgccc ggacggtttt gtagaagtcc tcgaaaaatt
      721 gctgcacacg ccggaaaagc gggtctccaa gcggaaactc tccgtaccaa accttttcat
      781 agatcatggt gaggtcccgg aatggcggtc cctgcggggt ttcgcctagc tgctgccagt
      841 attccctgtt ggtggcttcc tggtggtacg tgatcagccc cctctcattc agaagccgaa
      901 ggctcatcag gtacaggtac cgcaccgcct gccggtaatc acctgcctga agatattgtt
      961 gaagcctgcc ttcaaggtcg tcctcaatcg gactcggctc atggttcgcg atcggcgccg
     1021 cttgtcgttt cggcgaccgg tagaacaact ggagattatt gtcggcgatg atccggagaa
     1081 tagcatagag taagatggcg cccaacagga aaagaaggat gcgtcccgcc catttacccg
     1141 ttagcagcgt ccaaagccgg ctatcgcgtt tctcattccg gtcttcacgc cgccaatacc
     1201 tgggatcatt cgcatacgcg aaagctcttt cagcttgcca ttctgttacg gttgaatccg
     1261 cgaccctgcg cagtgcaggg cgttcagccg aatcggccgc cgttcgttcc cctgatgccg
     1321 cggagtccgc ggcggcccgc gcagggccaa ccgccggagc aaccgcgctg tcggcggcca
     1381 catccccgcg gacggtatcc tggaccgctg cgatcacgcg gccacacaag aacattccaa
     1441 gcaccagggt gcttagcgcg gcgcgatagc ggaccctcat atcgcgttca tttttttaat
     1501 cagctgtaca gggtagaaga caaaatacca gacaatgaaa ctgagggagc cccccaggat
     1561 aaacacgctc agtcataagg gcatggaaga ataacgggta acgaatcctt ccaggaaagc
     1621 cgcgacggtg aacaccggca ccagccccac cagcatcttc agtccgtccc tcgccccgcg
     1681 ccgaagggag tgcacccgct tatgcgtgcc ggggaacagg atactatttc ccagcacaag
     1741 gccggccgct cccgcgataa tgaacgacga gatctccagc gttccgtgaa tccagatcac
     1801 gagcacggat ttccacccga gtccatgcgc gaaaaacatg tactggaagg cgcctaccat
     1861 cacgccgttc tggaacagga gccaaaccgt tccaataccg agcagaaagc cggccacgaa
     1921 catcatgaaa gcgacctgga cattgttcag cacgatatac aggaacatat tcacgggatt
     1981 ttcctgcttg taaactccga aaggatctcc tttggaaata ttatcctcgg tcatatcgac
     2041 gtaattatcg ccgagcactc cgcgaacaaa ggattcgtca tgcgcggcgg aaaatatggc
     2101 cacaatcgca aaaagaacaa agatcagaaa tgagtagagg atctcccgcc ggtatctgaa
     2161 caccacctgc ggcaattcct ctttccaaaa ccggatgatc cgcgaagact cttctttctt
     2221 gttgcggtag atgccgagat agatcctgga cgataacccg ttcaaatatt gcgttaccct
     2281 gctgtggggg tagaatgttt tggcgtatcc cagatcgttc accagttcag tgaactggcc
     2341 ggccatctca tccggatcat tggcaggctg atattgatat tgcctccact tatcaatgtt
     2401 gtttttaata aacagacctt ctctcactgg aaatatttag gtgacgggat agccgggcgt
     2461 ctgggttatt tgcgctaaat ttacgggaat aaatataatc ctgaaaattg tgaccccgta
     2521 tgcttcaggt caagttggac accggcttta acatcgaggt cgattt
//
LOCUS       DATGKE010000674         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_357175, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000674 DATGKE010000000
VERSION     DATGKE010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357175"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VKU83_11405"
     CDS             complement(<1..839)
                     /locus_tag="VKU83_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94213.1"
                     /translation="MQRRSLKFTLKTQPSGSSLLQTPGDAKYFKFHKTRSVEQVTAKK
                     YEEIHFVDSTKSVWNYSLFTDEDVRNFQHGTHYRIYELMGSHRATVLGTEGYYFAVWA
                     PNATAVSVIGNFNNWQADSHPLYVRLDRSGIWEGFIPAFRTGEAYKYHIHGYRGGTLD
                     KGDPYAYFWEMRPQTASITWELDYDWRDSEWMRSRKQHNSLEAPWSIYEVHLASWMRP
                     DPGDEERYNSYQQIRDLLVPYVKEMGFTHVELMPVMEHPFDGSWGYQGTGYYAPTSRF
                     GDP"
     gene            897..>2354
                     /locus_tag="VKU83_11410"
     CDS             897..>2354
                     /locus_tag="VKU83_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLI94214.1"
                     /translation="MPKLTACTFLCLFLSVSVMAQSAQISVKIADTAEKKNLANSSVL
                     LLRKSDSIMVTFTRSGPKGDFHLTNLPRGQFLLLVTLPGYADYVDTLEVSDSNAIVRL
                     PVIGLVLKSKLLEAVVVRGRKGAMRIKGDTTEFLADSFHVQQGATVEDLLKKLPGIQV
                     DHNGQITAEGQTVKKVLVDGEEFFGDDPTLVTQNLRADMVDKVQVYDKKSDQAIFTGI
                     DDGQRDKTINLKLKESKKNGYFGRLTASAGTNGYYDEELMANYFKKKQKLAAYGILSN
                     TGKTGLNWQERDSYGQSMASNIDVDQNTGAVSFTSVATNNDLDNWSGQYSGQGYPKVA
                     TGGLHYNNKWADDGQSINGNYKYMDLSINGSSATNSENILPDTFFYSNSTQKFNRQII
                     RQNSSGIYEWKIDSTSSIKLMADGGNDHKINNELDSSEALASDSSLVNRNVRAIGTVG
                     DNMGFNSDLLWRKKLGKVGRTLSLSVRENYRDVTSS"
BASE COUNT          577 a          633 c          641 g          503 t
ORIGIN      
        1 tgagggtcgc caaaccgcga cgtaggagcg taatacccgg tgccctggta accccagctg
       61 ccgtcaaatg ggtgctccat caccggcatg agctccacgt gcgtaaaacc catttccttg
      121 acatagggca caagcaggtc acggatctgc tgatagctat tgtagcgctc ctcgtcgccc
      181 gggtccgggc gcatccagct ggccagatgc acttcgtaga tcgaccacgg ggcctcgaga
      241 gaattatgct gtttccggct ccgcatccac tcgctatcgc gccagtcgta atctagttcc
      301 caggtgatcg atgccgtctg gggccgcatt tcccagaaat aggcatacgg atcgcccttg
      361 tcgagcgtgc cgccgcggta cccatggata tggtatttgt aggcttcgcc ggtcctgaag
      421 gcgggaataa aaccttccca aatgcctgac cggtccagcc ggacatacag cggatggcta
      481 tcagcctgcc aattgttgaa attgccgatc accgacaccg ccgtcgcgtt aggcgcccaa
      541 accgcgaaat agtaaccctc ggtccccagc acggtagccc ggtgtgaacc catgagctca
      601 tagatccggt agtgtgtgcc gtgctggaaa ttacggacgt cctcatccgt aaacagcgaa
      661 taattccaca cggatttcgt ggagtccacg aaatggatct cttcatattt cttggcggtc
      721 acttgttcca cggatcgtgt tttatggaat ttgaaatatt ttgcgtctcc gggtgtctga
      781 agcaacgaac tgccggaagg ttgcgtcttt aacgtaaatt taagggatcg gcgctgcata
      841 ttgctgtttt tgcaaagtaa tttcattgtg tcctgatcaa taaccatcat tcctttatgc
      901 cgaaactgac cgcctgcacc tttctctgcc ttttccttag cgtttccgtg atggcgcagt
      961 cggcccagat ctcggtaaag atcgccgata cggcagaaaa aaagaatctc gccaacagct
     1021 ccgttctact cttacgcaaa tcggattcta tcatggtgac cttcacccgg tctggaccaa
     1081 aaggcgattt tcacctgacc aacctgcccc ggggtcaatt ccttttgctc gtcacgctcc
     1141 cgggatatgc ggattatgtc gacaccctgg aggtgagcga ttccaacgct attgtcaggc
     1201 tgccggtgat cggcctggtt ttgaagtcga agttgctcga ggcggttgtg gtccggggaa
     1261 ggaagggcgc catgcggatc aagggcgaca cgacggaatt tttggcggat agctttcatg
     1321 tgcagcaggg ggcgacagta gaggatctcc tcaagaagct gcccgggatc caggtcgacc
     1381 acaatgggca gatcacggcc gaggggcaga ccgtaaagaa agtcctggtg gacggcgaag
     1441 agttctttgg cgacgacccg accctggtga cccagaatct gcgcgcggat atggtcgaca
     1501 aagtgcaggt gtatgacaag aagagcgacc aggccatatt caccggcatc gatgatgggc
     1561 agcgggacaa gaccatcaac ctcaaactga aagaaagcaa aaagaacggg tatttcggga
     1621 ggcttaccgc cagcgccggg accaacggct actacgacga ggagctgatg gccaactact
     1681 tcaagaagaa gcagaagtta gcagcgtacg gcattctgtc caacaccggc aaaacggggt
     1741 tgaactggca ggaaagagac agctatggcc aaagtatggc aagcaatatc gacgtcgacc
     1801 aaaacaccgg agcggtttcc ttcaccagcg tggcgacgaa taacgacctc gacaattgga
     1861 gcggtcaata ttccggacag gggtatccca aagttgcaac cggaggcctc cattacaata
     1921 ataagtgggc tgatgacggc cagtcgatca acggcaacta caaatacatg gatttgtcga
     1981 tcaacggcag cagcgccacc aattcagaga atatcctgcc cgatacgttc ttttacagca
     2041 actccactca aaaattcaac cgccagatca tccgtcagaa ttccagcggg atctatgaat
     2101 ggaagatcga ttctacttcg tccatcaagc tgatggcgga cggcggcaat gaccacaaga
     2161 tcaataacga gctggactct tctgaagcgc tggccagcga cagcagcctg gttaaccgga
     2221 atgtccgcgc gattggcacc gtgggcgata atatgggttt taacagcgat ctgctgtggc
     2281 gcaagaaact gggcaaggtt ggacgaacgc tttccctcag cgtccgggag aactaccgcg
     2341 atgttacgtc gtcg
//
LOCUS       DATGKE010000675         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1101302, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000675 DATGKE010000000
VERSION     DATGKE010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101302"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VKU83_11415"
     CDS             complement(<1..984)
                     /locus_tag="VKU83_11415"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HLI94215.1"
                     /translation="MKRYLLFPILCLSALRAAAQPAIETYVKQHTVPVHSIDPDSTDF
                     ADLERIGEAIGDARIVMLGEQDHGDAPTFLAKSRLIRYLHEKKGFNVLAFESDFFGLN
                     NGWRPRLTAEELDTLERRNIYGLWTGCDACQTLFYRYLPATQQTEHPLMLAGIDNQMR
                     VPPLLKAVDSVVRALQLPVASGGNYESEVRPAIVNWGKNLKDSGLNRTYLGYLREIRA
                     ELAERQGTDGFWVQLIDNMMATDVELANRNNYVVSANTRDSAMASNLRWLCQSRYAGE
                     KIIVWAHNYHISKYSGHYSEALLKQERTMGTVFTSDPVLEKQTYVIGFTSYE"
     gene            complement(988..1965)
                     /locus_tag="VKU83_11420"
     CDS             complement(988..1965)
                     /locus_tag="VKU83_11420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HLI94216.1"
                     /translation="MQLQLDISFDPLPRPIRYGDKILLTGSCFTEHIGNALRDWKFHT
                     LQNPNGILFDAASVASSLVSYIERRVYTGNDLIYLNELWQSWQHHGLFSDVDRDQVLE
                     RINTSQERAHAFLREADWLIVTLGSAFSYRLAADGSSVANCHRAPGNTFVKHLMTIEE
                     IVTALDGCLHRLFYFNPKVQLIFTISPVRHIRDGVVENNRSKARLLESVHHLVGKFDR
                     LFYFPAYELVIDVLRDYRFYDIDMVHPNYAATQFVLEKFTEFGIHRDDRPVIEEVRKL
                     VTARRHRSLHPATEAHARFVREQHAKAEELAKRYPFLDLSDELAYFSND"
BASE COUNT          455 a          604 c          532 g          447 t
ORIGIN      
        1 ttcatacgac gtgaagccga tcacataggt ctgcttctcc aatacaggat cgctggtgaa
       61 caccgtcccc atcgtccgtt cctgcttcaa aagggcttcg ctataatgac cgctatattt
      121 ggaaatatgg tagttgtgcg cccatacgat gatcttttcg cctgcatacc ggctctgaca
      181 aagccaacgg aggttcgaag ccatcgcact gtccctggta ttcgccgaga ccacgtaatt
      241 gttcctattg gccaactcaa cgtcggtggc catcatattg tcgatcaact gtacccagaa
      301 tccatccgtt ccctgccgct ccgccagctc ggcgcggatc tccctgagat agcccaggta
      361 tgtacggtta agcccgctat ccttgaggtt cttaccccaa ttaacgatcg ccggccggac
      421 ctccgattcg tagttgccgc cggaggcgac cggcagctgc aatgcccgca cgacgctgtc
      481 gacggccttc agcaatggcg gaacgcgcat ctggttatcg atccccgcca gcatcagcgg
      541 atgttccgtt tgctgcgttg ccggcagata ccgatagaac aacgtctggc aggcgtcgca
      601 tccggtccat aacccataga tattcctacg ttccaaggtg tccagctctt ccgcggtaag
      661 cctcggccgc cagccattgt taagcccgaa aaaatcgctt tcgaaagcca gcacattgaa
      721 ccccttcttt tcatgcagat accggatcag ccgcgatttg gcaagaaagg taggcgcatc
      781 gccgtggtcc tgctccccga gcatgacgat ccgcgcatcg ccgatggctt ccccgatccg
      841 ctccagatcg gcaaaatccg tcgaatccgg gtcgatggaa tgcaccggga ccgtatgttg
      901 tttgacatac gtttctatgg ccggctgcgc cgccgcccgc agagcggata aacataagat
      961 cgggaatagt aaatagcgct tcatatgcta gtcattcgaa aaataagcca gctcgtcgct
     1021 cagatcgaga aaaggatacc gcttcgccag ctcctcggcc ttcgcatgct gctcgcgcac
     1081 gaaccgggca tgcgcttcgg ttgccggatg cagcgatcgg tgtcgccggg ccgtcaccag
     1141 cttccggacc tcttcgatca ccggccggtc atcgcgatgg atgccgaact ccgtaaattt
     1201 ctccagcacg aattgcgtcg ccgcgtagtt ggggtggacc atgtctatat cgtaaaaacg
     1261 atagtcgcgt agcacatcga tcaccagctc gtaggccggg aaatagaaaa gccggtcgaa
     1321 ttttcccacc aggtgatgga ccgattccaa cagccgggcc ttgctgcggt tattttccac
     1381 gacgccgtcg cggatatggc gcaccggact tatcgtaaag atgagctgaa ccttgggatt
     1441 gaaatagaat agccggtgga gacagccatc caacgccgtg acgatctctt cgatcgtcat
     1501 caggtgtttc acaaaggtgt tgccaggcgc ccggtgacag ttggccacag agctgccgtc
     1561 tgccgccagc cggtaagaaa aagcgctgcc taatgtaacg atcagccagt ccgcttcccg
     1621 caaaaaggca tgcgcccgtt cctgcgaagt attgatacgc tcgagcacct ggtcccggtc
     1681 gacgtcgcta aatagcccat gatgctgcca gctttgccat agctcattca aataaataag
     1741 gtcgttcccc gtataaaccc ggcgttcgat atacgataca aggctcgagg ccacgctggc
     1801 cgcatcgaac aggatcccat tggggttttg caacgtatgg aacttccagt cccgcagggc
     1861 attgccgata tgctcggtaa aacaggaacc ggtcagaagg atcttatccc catagcggat
     1921 aggccggggt aaagggtcga acgatatgtc tagctgtagt tgcactcact caaagatttc
     1981 ctcagcgagc cgcaggctcg catttaatgc tgtttcgtcc agcgccggca gcaggtgt
//
LOCUS       DATGKE010000676         9145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1182973, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000676 DATGKE010000000
VERSION     DATGKE010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182973"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VKU83_11425"
     CDS             <1..1977
                     /locus_tag="VKU83_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI94217.1"
                     /translation="GFVLAIGIVVDDAIVVVEAVQHYIDTEHLSAKEATARAIADITG
                     PVIAIALILASVFVPVGFIPGIVGRLYQQFAITIAISVLISAFVALSLTPALCMLLLR
                     EHHLSKESRGLNWFFFRFNNWFGRVTHHYTNGVRGWIKGAPLVIVLLTAIVIGDIFLF
                     ERKPTGFIPQEDEGRVYISFEIPEAGSTTRAITVLDSIQHIAQNVPGVLHTAALGGLN
                     ILTFGIKSNAGSIFIQFKPWNQRHGQAQQLQGIIAELQKRFAVIKDARIVVIPPPAIP
                     GLGNAGGFSIQIEQRQSNDDIHTFERVVNQFVAEANKEPEIGNAFTFFSAHTPGYEVD
                     VDRDKAKKLGLNLTDVYNTMQMYMGSTYINQLTIFGRNFYVVAQADTNFRADIHAIGN
                     YYVKNSNGEMIPLSAIVSYKQTETAPLISHFNIYRSAEVDGAAKTGYSSGQAIEALQR
                     VAARVLPEGYGFDWSGLSLQEIKSGNSAIYIFSLSVVFVFLFLAALYESFSVPFSVLL
                     AVPIGVFGAILTLWFIPRLTNNVYAQIGLITLIGLSAKNAILIVEFAKERVDRGMEVV
                     QATLDAVKLRLRPILMTSIAFLLGVLPLALATGAAKVARNTIGWTVLGGMFAATSLAI
                     FIVPVLFVVITRISYGKKKLEDLRNQDREYKLAE"
     gene            2082..2252
                     /locus_tag="VKU83_11430"
     CDS             2082..2252
                     /locus_tag="VKU83_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94218.1"
                     /translation="MDTLWISVCAFLVLGALGVFIEHLRVGKWLKNLLMWVIGGLMLL
                     SVVIGVIAAFFS"
     gene            2716..3405
                     /locus_tag="VKU83_11435"
     CDS             2716..3405
                     /locus_tag="VKU83_11435"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HLI94219.1"
                     /translation="MTLLIDIQYFPSITLYKKLYNLSSIVFEQYEAYQKSGFRNRCRI
                     AGAQGVVGLSVPLWKGRGQKTLTRDVKISNRQPWAARHWKTIMSCYSRSPWFDYYRAD
                     LEALYHGGKYDRLLDWDLACFEWSLRALDLPVTVGLTDSWRLDYDPGEVVDWRGRLRH
                     GGLDPGRLPAGGGPGGGSAAEIHDAAGEGRMAHRYRQVFEERTGFIPNLSILDLLFCE
                     GKQAIRYIRSS"
     gene            complement(3383..5140)
                     /locus_tag="VKU83_11440"
     CDS             complement(3383..5140)
                     /locus_tag="VKU83_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HLI94220.1"
                     /translation="MQVLTHFPILKALGWALVNSMWQMAALWVIYALFVTAFQNAAVR
                     HRFALLLLGAGALWTTASFVAAWLFPGSSGAFFLSILSPGQPAAGGFWQNTRRLVEDA
                     LPYGTSLYLLVLGSLLVRYGANYRHSRRLTRLGLSAMPPQFRTFVSATALQLGIRVPV
                     KAWLSSLVDVPLTLGLLKPVILLPAAMITHLTPQQVEAILVHELAHIRRRDYLLHLLI
                     TVLEGIFFFNPFARLLIRQLKKERENCCDDLVLQFKYDAHAYVSALLSLASRRPFGQK
                     LAVAATGTGDRMLLQRAKRILLPEKGERLRPGLRPLILLAVTALIAALPLYHPLLLSD
                     KPATGIAARTRVIYFVPPKRAAVRASSFAVEPAMPMRPVDLVITQPMILTAPRRVIRR
                     HGAGARLTGHRGNTDLEMAYDVRDPGNGSDLVVKTADDGLLFANSDGAPVAVTVAGGP
                     DPNAVPEATSRDFSMNIPQAAGPAAPTMDGTPFIPNSSFTFQYNEGDSSHSEEQLLYL
                     QQSAQLEVLAAMAKLQQQTAAQLKALTAQLSRATESLRLRNQIRSRQLQLQEDYFKKI
                     QSWQSKLQKSTHIRMIVYI"
     gene            complement(5143..5523)
                     /locus_tag="VKU83_11445"
     CDS             complement(5143..5523)
                     /locus_tag="VKU83_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HLI94221.1"
                     /translation="MSTRTIKPTESELEILQVLWVKNQASVREVHEELLKSKEAGYTT
                     TLKLMQIMHEKGLVRRDDSAKTHIYQASVSKERTQKHLLGKMINTLFGGSPTELVIQA
                     LGHHKASPAELEEIQQLLNNLKQQ"
     gene            complement(5748..7976)
                     /locus_tag="VKU83_11450"
     CDS             complement(5748..7976)
                     /locus_tag="VKU83_11450"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HLI94222.1"
                     /translation="MDKLIYLVPVMGFIGLLYTFVKSAWVSKQDPGNERMQEISKYIA
                     EGAMAFLKAEWKILSYFVVLMAILLAVMGASNPESHWAIALSFIAGAVLSATAGYIGM
                     RSATKANVRTAQAARSSLSKALSVSFTGGSVMGLGVTGLAIMGLSGLFIILKAIFAPH
                     AAVDSEEMRRTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGRETQSDDAFGGLSPVLLP
                     MFIAGVGILFSILGTLFVRIGESASLDTGVVQKALNMGNWGSIILTAIACVGLVAFFL
                     PDTMTLRGEEFTKWGVLAAIGVGLLVGTLMSLITEYYTAMGRGPVLSIIRQSGTGHAT
                     NVIGGLAMGMQSTFLPILVLAGGIWGSFACAGLYGVAIAAAGMMATTAMQLAIDAFGP
                     IADNAGGIAEMSELPKEVRERTDILDAVGNTTAATGKGFAIASAALTSLALFAAFVGV
                     AGIGGIDIYKAKVLASLFVGGMVPFVFSSLAIQAVGQAAMSMVEEVRRQFRTIPGIME
                     GTGKPEYDKCVAISTQASIKKMILPGAMAIFFPLFIGFVLGPEALGGFLAGATVCGVL
                     MGIFQNNAGGAWDNAKKSFEKGVEINGEIHYKKSEPHKASVTGDTVGDPFKDTSGPSM
                     NILIKLMSIVSLVIAPTLASLFTTNTDSGMRAPATHDAHQAAMSRPASASRVASHTVT
                     LR"
     gene            8396..>9145
                     /locus_tag="VKU83_11455"
     CDS             8396..>9145
                     /locus_tag="VKU83_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94223.1"
                     /translation="MMPFKKLLGVLLAVIPGCFSLDALGQACTVLGQTPETAFPVCGS
                     NIFTQDSVPICNDGGVPVPGCGAGYSAINPYWYKFTCFSSGTLGMLISPANQGDDYDW
                     EIFDVTGQPLSAVYTNINLVVADNWSGVYGNTGTSQTAVGNSECGSVGNPPYTTPPPF
                     SAMPYLIQGHDYLLLISHFSGSGQSGYQLSFSGGTASITDTTQPLVNSANAVCLSVVG
                     VRLNKKMSCSSLTASGSEFSISPLPPGITIVS"
BASE COUNT         2025 a         2442 c         2640 g         2038 t
ORIGIN      
        1 ggattcgtcc tcgcgatcgg gatcgtcgtg gatgacgcca tcgtcgtggt tgaggcggtg
       61 cagcattata tcgatacgga acacctctcg gcgaaggaag cgacggcgcg tgccattgcg
      121 gacattacgg ggccggtaat cgctattgcg ctgatcctcg cctcggtatt cgtaccggta
      181 ggttttattc ccggtatcgt ggggcggctt tatcagcaat ttgcgattac aatcgcgatc
      241 tcggtgttga tctcggcctt tgtagccttg tccctgacgc ctgcgctatg tatgttgctg
      301 ctccgcgagc accatctcag taaggaatcc cgcggattga actggttttt cttccggttc
      361 aacaactggt tcgggcgggt cacacatcat tatacgaatg gagtgagggg ctggatcaag
      421 ggcgcgccgc tggtcatcgt gctgctaact gctatcgtga tcggcgatat cttcctgttc
      481 gagagaaaac cgaccggctt tattccgcag gaagacgagg gcagggttta tatctcgttc
      541 gagatcccgg aagctggttc caccacaagg gccattaccg tcctggacag cattcagcat
      601 atcgcgcaaa atgtgccggg cgttttacat acggcggcgc tcggcgggtt gaacatcttg
      661 acatttggga tcaaatcgaa cgccggcagc atttttattc agttcaagcc ctggaaccaa
      721 cgccatggac aggcgcagca gttgcagggc atcattgcgg agctgcaaaa gcgttttgcc
      781 gtaattaagg atgcccgcat cgtcgtgatc ccgccgccag cgattcccgg cctgggcaac
      841 gccggcggtt tcagcatcca gatcgagcag cggcaatcga acgacgacat tcacaccttc
      901 gaaagggttg tgaaccagtt tgtagccgag gccaacaaag agccggagat cgggaacgcc
      961 tttacctttt tcagcgcgca taccccggga tacgaagtcg acgtagaccg ggacaaggct
     1021 aagaagctcg ggctgaacct gacggatgtt tataacacga tgcaaatgta tatgggcagc
     1081 acttatatca accagctgac catctttggg cgcaacttct atgtagtggc gcaagccgac
     1141 acgaatttcc gcgccgatat ccatgccatc ggaaactatt atgtcaagaa ttcgaatggt
     1201 gaaatgatac ccttgagcgc tattgtttcc tataaacaaa cggagacggc gccgttgatc
     1261 tctcacttca acatctaccg gagcgcggaa gtggatggcg ccgcaaaaac cgggtacagc
     1321 agcggtcagg ccatcgaggc gctgcagcgg gtggcggccc gcgtgcttcc ggagggctac
     1381 ggctttgact ggtccggcct cagcttgcag gagatcaagt ccggcaatag tgcaatctat
     1441 attttttcgc tgtcggtcgt gttcgtgttc ctgttcctgg ctgcgctgta tgagagcttc
     1501 tcggtgccgt tttcggtcct gttggcggtg ccgataggcg tttttggtgc tatattgaca
     1561 ttgtggttca tcccccgcct taccaacaat gtatatgcgc agatcggatt gataacgctg
     1621 atcggccttt cggcgaagaa tgcgatcctg atcgtagaat tcgccaagga gcgggtggac
     1681 aggggaatgg aggtcgtgca ggccacactg gacgcggtta aattgcggct aagaccgatc
     1741 ctgatgacct cgatagcctt cttgctcggg gtactgccac tggcgttggc cacgggagcc
     1801 gccaaagttg cccggaacac gatcggctgg acagttctcg gcggtatgtt tgcggctacg
     1861 agcctcgcga tttttatcgt tccggtactt ttcgtagtca tcacccgtat cagttacgga
     1921 aaaaagaaac tggaggatct tcggaaccag gacagggagt ataagctggc cgaatgaccc
     1981 ggaatttaga aaatttacta ttttcaggcg gaaaatggtg acttggatcc gctggacgac
     2041 ccttaaatga tctgttgaca tcttaacaat actcctgact gttggatacg ctttggattt
     2101 ctgtttgtgc attcctcgtc ctcggggcgc tgggcgtttt tatcgaacac ctccgggtgg
     2161 gcaaatggtt gaagaacctg ctcatgtggg tgatcggcgg cctgatgctg cttagcgttg
     2221 taatcggggt catcgccgct ttcttttcat agccgggttt tcttcggacg tgcgccgcgt
     2281 cggaaccggc gccattgcct gaaacctgga gcagttgacc ggtcaattgc aaaacggtaa
     2341 tccccacttg tcagcggcat cgcggccaaa ttccatccaa agacaccagg gtaggggcct
     2401 gggccattcc catcccgttt tccaaatcca ttacttttgc gctgtggggc cgcgatatag
     2461 tgagctcaat cgtgaccctt cttccgggca attttctatg ctaatattgt tgggaatttg
     2521 ttttagctgc agggaaaaat atccggagtt tcttactaat atagctagtc ttggaatcaa
     2581 gacattaaac ttatggtttt gaaacatcta tcaaatatta tatcataatt aatttacaga
     2641 tcatcggcgc gaatcctgcc tttcggcctg catttttcgg tccgttttgg acacagctga
     2701 taaataatat ataccatgac attattaatt gatatacaat actttccttc gattacttta
     2761 tataaaaagt tatataactt gtcgagtatt gtttttgagc aatatgaagc ctatcagaaa
     2821 tccggttttc gcaaccgttg ccgcatcgcc ggcgcgcagg gtgtggtggg attgagtgtg
     2881 ccgctatgga aagggagggg ccaaaagacg ctcacccgcg atgtgaagat atcgaaccgc
     2941 cagccctggg ccgcccggca ttggaagacc atcatgtcct gttacagccg gtcgccctgg
     3001 tttgattatt accgggcgga cctggaagcg ttataccacg gcggtaaata tgaccggtta
     3061 ctggactggg acctggcatg cttcgaatgg tcccttcggg cgctggacct ccctgtgacg
     3121 gtcggactta cggactcctg gcggctggat tacgatcccg gcgaagtggt ggactggcgc
     3181 gggcggctcc ggcacggcgg acttgacccg ggaagacttc cggctggagg tgggccgggt
     3241 ggtggcagcg cggcggaaat tcatgatgcc gcgggggaag ggcgcatggc gcatcgttac
     3301 cggcaggtct tcgaggaaag aaccggcttc attcccaatc tttcgatcct ggacctgttg
     3361 ttttgcgagg gcaagcaggc gatcagatat atacgatcat cctgatatgg gtggacttct
     3421 gaagcttcga ttgccagctc tggatctttt tgaaataatc ctcctggagc tgcaactggc
     3481 gggaccggat ctggttgcgg agccgcagtg actcggttgc cctggagagc tgcgctgtaa
     3541 gggccttcag ctgcgcggcc gtttgctgct ggagcttggc catcgcggcg agcacctcca
     3601 gctgggccga ttgctgcaag taaagcagtt gttcctcgga atgtgaactg tcgccttcat
     3661 tatactggaa tgtaaaactc gagttgggta taaatggcgt tccgtccatt gtgggggcgg
     3721 cggggccggc cgcctgggga atgttcatgg aaaagtcgcg gctggtcgct tcaggtacgg
     3781 cattcggatc gggtccgccg gccaccgtaa cggctacagg tgccccgtcg gagttggcaa
     3841 agaggagtcc atcgtcagcc gttttcacaa caagatcgct tccattcccg gggtcccgca
     3901 catcgtatgc catttccagg tcggtattgc cccgatggcc ggtcagccgg gcacctgcgc
     3961 catggcgccg gattacccgc cgcggcgcgg ttaaaatcat tggctgggta attaccagat
     4021 ccactgggcg catgggcatc gccggttcga cggcaaagct tgatgccctg actgccgctc
     4081 tctttggagg aacgaaataa atcacgcgcg tccgggccgc gatgcccgtg gcaggcttgt
     4141 ctgaaagcag cagaggatgg tataaaggca gggccgcgat caaagcagta acagccagca
     4201 ggatcaaggg gcggaggcca ggacgcagac gttccccttt ctcaggaagc aggatccgtt
     4261 ttgcgcgctg aagcagcatc cggtcgccgg tcccggtggc cgctaccgcc agtttttgcc
     4321 cgaacggacg gcgggacgcg agcgacagca gcgccgatac atacgcgtgc gcgtcatatt
     4381 tgaattgcag gacaaggtcg tcgcaacagt tttcccgttc ttttttcaat tgccggatca
     4441 gcagccgggc aaagggattg aaaaaaaaga ttccctccag gaccgttatc agcagatgaa
     4501 gcagataatc ccttcgacgg atatgggcaa gctcgtgaac caggatcgct tcgacctgtt
     4561 gcggcgtcaa atgggtgatc atggccgccg gaagcaggat aacgggtttc agcaagccca
     4621 gcgtcagcgg aacgtcgacc agggaggaaa gccaggcctt gacgggcacc cggatgccga
     4681 gttgcagggc cgtcgcgctg acaaaggtcc tgaattgcgg cggcatggct gaaagcccaa
     4741 gccgcgtcag gcgcctcgaa tggcggtaat tggcgccgta tcgaacaagc aaagaaccaa
     4801 ggacaaggag gtacagtgaa gttccgtaag gcaacgcgtc ctccaccagg cggcgggtgt
     4861 tttgccaaaa cccgcctgcg gcgggctggc cgggggacag gattgaaaga aaaaaagccc
     4921 cggaacttcc cggaaaaagc caggcagcaa cgaaactcgc cgtcgtccaa agggcgccgg
     4981 cgcccagcag gagcaaggca aaccggtgcc tgacagcagc attttgaaag gcggtaacga
     5041 acagggcgta gatcacccaa agcgcggcca tctgccacat gctattgacg agagcccagc
     5101 ccagcgcctt cagtatgggg aaatgggtca agacctgcat gcctattgtt gtttcaggtt
     5161 gttcaaaagc tgttggatct cctccaactc ggccggcgaa gctttatgat gtccaagggc
     5221 ctgaatgacc aattcggtag gcgaaccgcc gaaaagcgta ttgatcattt ttcccagcag
     5281 atgtttctgc gttctttcct tgctcacgga agcctggtaa atatgggttt tggcggagtc
     5341 gtcacgccta accagccctt tttcgtgcat gatctgcatc agtttcagtg tcgtagtata
     5401 gccggcttcc ttcgatttga gaagctcttc atggacctcc ctgacgctcg cctggttctt
     5461 tacccagagg acctgcagga tctccagttc gctctcggtg ggtttgattg tccttgtcga
     5521 catagaaagg attttgaccg acaatttacg acattattcg taaatcattg ttaaagcggg
     5581 gcaaaaattg aggccggggg gcaactgcaa aaagaaaaag ccccgatgtg gaaacaccgg
     5641 gactttggta tttggatctg attagtgttg ttgaagtgat aaagatatac attcagcgga
     5701 caaacctcac aaaaaaccgg gttttttttg ttaacgccag ccgggttcta tcgaagggtc
     5761 acggtgtggc tggcaaccct ggaggccgag gccggccggg acatcgcggc ttgatgcgcg
     5821 tcgtgtgttg ccggcgcccg catgccggag tcggtgtttg ttgtaaacag gctcgccagt
     5881 gtaggggcga tgacaagcga cacgatggac attaatttga tcaggatatt catcgaaggg
     5941 ccggaagtat ccttgaatgg gtctccgacg gtgtcaccgg tgacagacgc cttatgcggc
     6001 tcggacttct tgtaatgaat ctcgccattg atctcgacac ctttctcgaa ggatttttta
     6061 gcattatccc aggcgccgcc ggcattgttc tggaagatgc ccatcaggac accacagacc
     6121 gtggcgcctg cgaggaatcc accgagagct tccggaccga gaacgaatcc gatgaacaac
     6181 gggaaaaaga tggccatcgc cccgggcagg atcattttct ttatggatgc ctgcgtcgaa
     6241 atcgcgacgc atttatcata ttcgggcttg ccggtgcctt ccataatgcc ggggatagtc
     6301 cggaactgac ggcggacttc ctccaccatc gacatggccg cctggccgac agcctggatc
     6361 gccagggaag aaaagacgaa gggcaccatc ccgcccacga acaggctcgc gagaacttta
     6421 gctttataaa tgtcaatccc cccgatgccg gcgactccta cgaaggcagc aaataaggca
     6481 agggaagtga gggcggccga cgcaatggca aatcccttcc ctgtcgcggc ggtcgtattg
     6541 cccacggcat cgaggatatc cgtcctctcg cgtacctcct taggcagctc gctcatctcg
     6601 gcaatgccgc cggcgttatc ggcaatggga ccaaacgcgt cgatagccag ctgcatagcg
     6661 gtagtggcca tcatgccggc cgctgctatc gcaacgccat acaggcctgc gcatgcaaac
     6721 gatccccaga tgcctccggc gagaacgagg attggcagaa atgtagattg cattcccatc
     6781 gccagaccgc cgatgacgtt ggtggcgtgg cccgttcctg attggcgaat gatactcagc
     6841 acaggccccc ggcccattgc cgtgtaatat tctgtgatca ggctcatcag ggtacccacg
     6901 agcaggccga cgccgatggc ggcaagcact ccccatttag tgaattcttc ccctctgagg
     6961 gtcatggtgt ccggcaggaa aaacgcgacg aggccaacac acgcgatggc ggtcaaaatg
     7021 atcgagcccc agttgcccat attcagggct ttctggacga cgccggtgtc aagactggcc
     7081 gattcgccga tccggacgaa taatgttccc aggatcgaaa agaggatgcc gacaccggcg
     7141 atgaacatgg gcaaaagaac aggcgataaa ccgccgaaag catcgtcgga ctgggtctcg
     7201 cggccaagca ccatcgtcgc cagtacggtg gcgacatagc tgccgaaaag gtcggcgccc
     7261 attccggcga cgtctcccac gttatcgccg acattgtccg ctatcgtggc agggttccgc
     7321 ggatcatcct cgggaatacc tgcctccact ttgcccacca ggtcggcgcc gacgtctgct
     7381 gcctttgtat aaatacctcc gccaacgcgc gcaaaaagcg cgatggattc ggcgcccagc
     7441 gagaatccgg tcaatacctc gatcgttctt cgcatttctt cggaatcgac cgcggcatga
     7501 ggagcgaaaa tcgctttgag gatgataaag agaccgctca ggcccattat ggccaggccg
     7561 gtcaccccga gacccattac cgagccgccg gtgaaggaga cggaaagcgc tttgctgaga
     7621 ctgctccgcg cagcctgggc ggtgcggaca ttggcctttg tggcgctacg catcccgata
     7681 tagccggcag ttgcgctcag caccgcgcct gcgatgaagg aaagggcgat agcccagtgg
     7741 gattcggggt tgctggcgcc catgacagca aggaggatcg ccataaggac cacgaaatag
     7801 gaaagtatct tccattcagc cttcaaaaat gccatggcgc cctcggcgat atacttgctg
     7861 atctcctgca tccgttcgtt accgggatct tgtttggaaa cccatgccga cttaacaaag
     7921 gtgtacagga gaccaataaa tcccatcacg ggaaccagat aaatcaattt gtccataagt
     7981 tgtcaatcaa tcttagtgaa tcgccccggt ctcgcggtta agaatggtgg cccgagggac
     8041 ggcaaattta ggggaaaaga agacagccgc ggacatatag gcaaaaaaac tggcttctgc
     8101 tttccagctt cagtgttcac ggcaatgcgg gaacggctgt aaagcggaaa cccctccggg
     8161 taggaagggc ttcgaaggct tggcttgggt ttagggttta gggttcaggg tacacgatat
     8221 ggacgaaata tgtgccacga cttcaatttg ctggttttca tatatttgta ggataagatt
     8281 tcgcgtttgg ttcccgattc cggaaaagcg ggtcccggaa ggggaccggc gcggctcgag
     8341 aaacttggca acgacgcaac ggcgggcctg ttcccgccgt cttgaataaa gctgtatgat
     8401 gcccttcaaa aagctgttag gtgttttatt ggccgtcatc ccaggctgct tcagcctgga
     8461 tgccctcgga caagcgtgta cggtgctcgg gcagacgccc gaaacggcgt ttccggtctg
     8521 cggcagcaac atattcacgc aggacagtgt ccccatttgc aatgacggtg gcgtcccggt
     8581 tccaggctgc ggcgccgggt attcggccat caacccctac tggtataaat tcacctgctt
     8641 ttcttcggga acgctgggca tgctgatcag ccccgcgaac cagggagatg actacgattg
     8701 ggagatcttt gacgtcacgg gccagccttt gagcgcggtt tacaccaaca tcaatctggt
     8761 ggtggccgac aactggtccg gagtttatgg gaacaccggc acatcgcaga ccgccgtcgg
     8821 caattccgaa tgtggttcgg tcgggaatcc cccctataca acaccgccgc cgttcagtgc
     8881 gatgccgtac ctgatccagg ggcacgatta tctcttgctg atcagccact tcagcggcag
     8941 cggccagagt ggctatcagc tctcgttctc cggcggcacg gcgagcatca ccgataccac
     9001 acagccgctt gtgaattcgg ccaacgcagt ctgcctttcg gtcgtcggcg tgcgtttgaa
     9061 caaaaagatg agctgcagca gcctgacggc aagcggcagc gaattcagca tctcacccct
     9121 gccgcccggt ataacgatcg taagc
//
LOCUS       DATGKE010000677         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_492667, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000677 DATGKE010000000
VERSION     DATGKE010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492667"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VKU83_11460"
     CDS             <1..1285
                     /locus_tag="VKU83_11460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLI94224.1"
                     /translation="TSKSFTAAAVGFALTENKLQLSDRIVSFFPGDLPQPVSPFLAEL
                     TVRDALMMADGQEPDPTSSVVNDSNWARKFLSIPILHEPGTTFLYNSAGTYMLAAVVQ
                     KVTGQTVLEYLKPRLFEPLGISGEDWETSPQGVNTGGWGLRLRTEDMAKFGQLYLQGG
                     RWNGKQVLPASWVKEATSVKIMQDPNAPQSKKDSSDWLQGYCYQMWRCRHNGVRADGA
                     FGQFIIMLPDEDAVVAITAETPDMQEEINLVWQYILPALHPATLPSDPGADARLRRQL
                     TALALSTAPKDAGPAGNPFDGKSFDLAANSKRMKAMAFHFSGHRLELDITGDTAHYRI
                     AFGDGRWIAGTTTRPGPDLLAGAVHHDVGLPAFHTAGSYQWVDGHTLRLVLRYIESPH
                     SETMICTVEGNRLSVEDANSFQFGKNTLSIQGTAQ"
     gene            complement(1378..1860)
                     /locus_tag="VKU83_11465"
     CDS             complement(1378..1860)
                     /locus_tag="VKU83_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94225.1"
                     /translation="MNKFFLAATLIAATGLTNPSQAQISFTINIGDQPAWGPTGYDHV
                     EYYYIPDIQAYYYVPSHEFVYLNGGHWTFSRALPPRYHDFDLFHCRKIVMNDPQPYLH
                     FDEHRRDYDHFRGNPQGQEFIRDSHDDRYKWHWHDQHEADEEWRHRPEGDRHWHEDRH
                     "
     gene            complement(1928..>2154)
                     /locus_tag="VKU83_11470"
     CDS             complement(1928..>2154)
                     /locus_tag="VKU83_11470"
                     /inference="COORDINATES: protein motif:HMM:NF042710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HLI94226.1"
                     /translation="VNEKVRKKFDAKYTEVGGTAQVGNKVMMKKNHQVGTVREIRGKK
                     AIVQVGLVPMTVDLAELTVVQDRQQEESAG"
BASE COUNT          464 a          640 c          592 g          458 t
ORIGIN      
        1 aaccagcaag agcttcacag cggccgcggt gggcttcgcc ctgacggaaa acaagctgca
       61 gcttagcgac aggatcgtga gctttttccc gggcgacttg ccgcaacccg taagcccgtt
      121 tctcgcggaa ctgacggtca gggacgcctt gatgatggct gatggccagg aacccgatcc
      181 tacttccagt gtcgtgaacg actcgaactg ggcgagaaaa ttcctttcca tacccattct
      241 ccatgagccc ggcacgacct tcctttacaa ttccgcaggc acctatatgc tggccgccgt
      301 ggtacaaaag gtcaccgggc aaaccgttct cgaatatttg aaaccgcggc tatttgaacc
      361 gttgggcatc agcggcgaag actgggagac cagcccgcag ggcgtcaaca ccggcggctg
      421 ggggctccgg ctcaggacgg aagatatggc gaaattcggc cagctctatc tgcagggcgg
      481 acgctggaac gggaaacaag tcttgcctgc gtcatgggta aaggaggcga cgagcgtgaa
      541 gatcatgcag gacccgaatg cgccgcagag caaaaaggat tccagcgact ggctccaggg
      601 ttactgttac caaatgtggc gctgccggca taacggcgtg cgtgccgatg gcgctttcgg
      661 tcagtttatt atcatgctgc ccgacgagga cgcggtcgtt gccatcaccg cagaaacgcc
      721 cgacatgcag gaagagatca acctggtatg gcaatacatc cttcccgcac tgcatcccgc
      781 cacgctgcca tccgatccgg gagccgacgc acgactccgg cggcagctca ctgccctggc
      841 gctgtcgacc gcgccgaagg acgcaggtcc cgccggcaat ccgttcgatg gcaaatcatt
      901 cgacctcgct gccaattcca aaaggatgaa ggcgatggcc ttccactttt ccggccacag
      961 gttggagctg gatattacgg gcgataccgc tcattacagg atcgccttcg gcgacggccg
     1021 ctggatcgcc ggtacaacga cccgaccggg ccccgacctt ctagcggggg cggttcacca
     1081 tgacgtcgga ttgccggcct ttcataccgc cgggagttac cagtgggtcg acggccatac
     1141 cctgagactg gtcctccgct atatcgaaag cccccatagc gaaaccatga tctgtaccgt
     1201 cgagggcaat cgactatctg tcgaggacgc gaacagtttt caatttggga aaaacaccct
     1261 cagcatccag ggcaccgcgc aataaaaaac cccggccggt gccagggttg ctaaagttaa
     1321 ggttgacgcc gaaacgcccg atgccgaatc gccgtacgcc ggaccgccgg tgatcgctca
     1381 gtgccggtcc tcatgccaat gccggtcgcc ttccgggcga tgcctccatt cctcatccgc
     1441 ttcatgctga tcgtgccaat gccatttgta gcgatcgtcg tggctgtccc ggataaattc
     1501 ctgcccttgc gggtttccgc gaaaatgatc gtaatccctc cggtgttcgt caaagtgcaa
     1561 gtaaggctgc ggatcattca ttactatttt ccggcagtgg aagaggtcaa aatcatggta
     1621 ccggggaggc aatgcccggg aaaaagtcca gtggcctccg ttcagataca caaactcgtg
     1681 ggacggaaca tagtagtaag cctgaatgtc gggaatgtaa taatattcca catggtcata
     1741 cccggtcggc ccccaggctg gctgatcgcc aatgttgatc gtgaaactaa tttgcgcctg
     1801 gctcgggttc gtgaggccag ttgccgcaat cagcgttgcg gcgaggaaaa atttattcat
     1861 gcgtgagaga tttaagttag ccattcaccg gcaaagtcaa tgccaaatta accgctggct
     1921 gcggacctta tcccgcgctt tcctcctgtt gcctgtcctg cactacagtc agttccgcaa
     1981 ggtctacagt catcgggacc agccccacct gcacgatggc ttttttgccg cggatctcgc
     2041 gcaccgtgcc gacctggtga ttctttttca tcattacctt attgccgacc tgcgccgtgc
     2101 cgccgacctc agtgtatttg gcgtcaaatt tttttcttac tttttcattg acct
//
LOCUS       DATGKE010000678         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1011103, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000678 DATGKE010000000
VERSION     DATGKE010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011103"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..874)
                     /locus_tag="VKU83_11475"
     CDS             complement(77..874)
                     /locus_tag="VKU83_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLI94227.1"
                     /translation="MNRQFFDQWAIILGGSSGFGLAAVEKLAIHGMNIAVLYRETGAA
                     DRLLKEKFNGLSSDAGVRIDAYNLNALDPDSRQIFLQQFAERAGRPHCVRLLLHSIAR
                     GNLKPLLDDAAAEGLTVDDIRFTTYAMSTSLLDWARLLLKEGHFQQDARIIGLTSEGA
                     HKYWDGYAAVSIAKSTLESLVTYMAVELGKYGLKTNLIQAGISATPSLRKIPSSERLL
                     ELATARNPLGRTTRPEDVAGAIYLLCLDEASWINGSIIPVDGGEHCR"
     gene            982..1476
                     /locus_tag="VKU83_11480"
     CDS             982..1476
                     /locus_tag="VKU83_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94228.1"
                     /translation="MKRSLIIACISILSGTAIGAAPAFQSFDKARFYSVMASGKIGAV
                     DSELSIVAVATVAEKEAYEGALLMRKAGLLSGPREKLVTFRSGAVKLERSLARDSANA
                     EYHFLRLMIQEHAPAIVHYNKDKERDSRVVIRAFPRLPPVLQNAIRDYWPYSRTLQET
                     KLNG"
     gene            1469..2368
                     /locus_tag="VKU83_11485"
     CDS             1469..2368
                     /locus_tag="VKU83_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94229.1"
                     /translation="MGKKVLAIFYSQSGQLAEIIGSLVAPLEAGGASVETVRIEPVTP
                     FDFPWTKDRFYDVMPDCVHRATVELAPFTLKQSAYDLIVLGYQAWFLYPSLPVNSLLE
                     HPAFRSVLKNTPVVTVTGARNMWLNAFLELKRSLHSLGARLVGNIALIDRHPNHISFF
                     TIFHWLLRGRKDRMMKIFPYPGVSAADIANASAYGKLMLPYLQRDEWEGLQGELVRAK
                     AVSTRYSLWLLESRAVPTYVVWAGFITRRKKRKAWLSVFRYYLLFSLFVAAPIMLLVD
                     AAFIRPFSSNRIRKKRQYYLSLT"
     gene            2379..>2680
                     /locus_tag="VKU83_11490"
     CDS             2379..>2680
                     /locus_tag="VKU83_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94230.1"
                     /translation="MSRPVYITKTSIFMPNEPVSCDDMELYLGYINGKPSKSKKIVLR
                     NNGIKTRYYALTKEGKATHTNAQMTALAVRALFENDPARLKEIELLSCGTSSPDQM"
BASE COUNT          614 a          713 c          730 g          623 t
ORIGIN      
        1 ccacaaaacg aaagctggac ttatagggaa gcaggtcaag aatatactgg tactgcatat
       61 gtcagggatt agaagactac cggcaatgtt cgcctccgtc caccgggatg atggagccgt
      121 tgatccagga cgcttcgtcg aggcaaagca ggtagatcgc gcccgcgacg tcctcgggcc
      181 ttgttgtccg gcccagggga ttccttgccg tggcaagttc cagcagccgc tcgctggagg
      241 ggattttcct gagcgagggc gtagcagaga tccccgcctg tataaggttg gttttcaacc
      301 catattttcc gagctccacc gccatatacg taacaaggct ttccagcgtc gacttggcga
      361 tggagacggc ggcataacca tcccagtatt tgtgcgcccc ttcgctggtc aggccgatga
      421 tccgggcatc ctgctgaaaa tgtccttcct tcaacagcaa ccttgcccag tcgagcaggc
      481 tggtggacat cgcgtaggtc gtgaaacgga tatcgtccac cgtgagacct tcagccgctg
      541 cgtcgtccag cagcggcttg agatttcccc tggcgatgga atgaagaaga agtcgaacgc
      601 aatgcggccg gccggcccgt tctgcgaatt gctgcaggaa gatctgccgc gaatccggat
      661 caagcgcatt caggttatag gcatcgatgc gcacccccgc atccgacgag aggccgttga
      721 acttctcttt caacaaacgg tctgcggcgc cggtttcccg gtaaagaacg gcaatgttca
      781 tgccatggat cgccagcttt tccactgctg cgaggccaaa tccgctggaa ccgccgagga
      841 tgatagccca ctgatcaaaa aattgtctgt tcatcaaaat agctcgcaaa attaaggaaa
      901 cttgttaata cgatgaaagt taattattta tatttggcaa ggagatctta ttctttatat
      961 ttgccacatt tccctccggc catgaaacga tctctcatta tcgcctgtat atccatattg
     1021 tccggaactg ccatcggcgc cgcgcccgcc ttccaatcct ttgataaagc gaggttctat
     1081 tcggtcatgg cttcgggtaa aatcggcgcg gtcgattccg aactctcgat agtggcagtt
     1141 gcgacagtag cagaaaaaga agcctatgaa ggggcattgc tgatgcggaa ggcaggcctg
     1201 ctcagcggcc cgagggaaaa gcttgtcact ttccgatccg gcgccgtcaa gctggagcga
     1261 tcgctggccc gcgacagcgc gaacgcggag tatcattttt tgcgtttgat gatccaggag
     1321 cacgctccgg cgatagtcca ttataacaaa gacaaggaac gcgatagccg ggtggtgatc
     1381 cgggcctttc cccggttgcc gcccgtcctg caaaatgcca tccgcgatta ttggccttat
     1441 tccagaacgt tgcaagaaac caaactgaat gggtaagaag gtattggcca tcttttattc
     1501 acaatcgggg cagctggcgg aaatcatcgg ctccctggtg gcgccgctgg aagcgggggg
     1561 cgcatccgtc gagaccgtcc ggatcgagcc ggttaccccc ttcgattttc cctggacgaa
     1621 agaccgcttc tatgacgtca tgcctgactg cgtccaccgg gcgacggttg aattggcgcc
     1681 gttcacgctg aagcaatctg catacgacct gatcgtcctc gggtatcagg cctggttcct
     1741 gtatcccagc cttcccgtga atagcttatt ggagcatccg gctttccgga gcgtgctgaa
     1801 gaacaccccg gtagtgacgg tcaccggcgc gaggaatatg tggctcaatg cttttctgga
     1861 actgaaaaga tcccttcatt cgctgggggc ccggctggtg ggcaatatcg cgctgatcga
     1921 ccgccatccg aaccatatca gcttcttcac catttttcat tggctgctga ggggacggaa
     1981 ggaccggatg atgaagatct tcccctatcc gggggtttca gcggcggata ttgcgaatgc
     2041 ttcggcatat ggcaagttga tgctgcctta tttgcagcgg gatgaatggg aagggctgca
     2101 gggcgagctg gttagggcga aagccgtctc cacgcgctat tcgctttggc ttttggaatc
     2161 acgcgccgtt cccacttacg tagtgtgggc gggcttcatc acgaggcgga aaaaaagaaa
     2221 ggcatggctc tccgtattca gatattatct gctattttca ctctttgttg cggctccaat
     2281 catgctgctt gtcgacgcgg cttttatcag gccattttcg tcgaatcgga taaggaagaa
     2341 aaggcaatat tatttatcgc tgacctaagc tcaagattat gtcccgacct gtatatatca
     2401 ccaagacgtc catcttcatg cccaacgagc ccgtttcctg tgacgacatg gagctgtacc
     2461 tcggctatat caatggcaaa ccatccaagt cgaaaaagat cgtcttaagg aataacggca
     2521 tcaagacaag gtattatgcg ttgacaaaag aaggaaaggc tacgcatacc aacgctcaaa
     2581 tgaccgcgct ggcggtcagg gcgctgttcg aaaacgaccc tgcgagactg aaggagatcg
     2641 agttgttgtc ctgcggtacc tcttcgccgg atcaaatgat
//
LOCUS       DATGKE010000679         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1078303, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000679 DATGKE010000000
VERSION     DATGKE010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078303"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VKU83_11495"
     CDS             complement(<1..1084)
                     /locus_tag="VKU83_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLI94231.1"
                     /translation="MSQLTSTPSKPKAAATAGDRKSAAQPTAPRELSGSAAVLEAFLE
                     EGVDTIFGYPGGAIMPIYDALYDYRDRLNHILVRHEQGAVHAAQGYARTSGRTGVVFA
                     TSGPGATNLVTGLADAMIDSTPVVCITGQVFAHLLGTDAFQETDVINITTPVTKWNYQ
                     VTDATEIPSVLAKAFYIAGSGRPGPVLIDITKNAQLQKFSFAGYSRCEHIRSYRPKPI
                     IRKEFVDKAAQLINEAERPFVVFGQGVILGKAEQEFRDFIEKGGIPAAWTILGLSALP
                     TSHPLNVGMLGMHGNYGPNVLTNDCDVLIAVGMRFDDRVTGRLDKYAKQAKIVHLDID
                     PAEIDKNVKSAVPVWGDCKETLPLLTA"
     gene            complement(1130..2809)
                     /gene="ilvD"
                     /locus_tag="VKU83_11500"
     CDS             complement(1130..2809)
                     /gene="ilvD"
                     /locus_tag="VKU83_11500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLI94232.1"
                     /translation="MELNHYSKTLTQDVTQPAAQAMLYGIGLTDKDLAKAQVGIASMG
                     YDGNTCNMHLNDLAQLVKKGVWEKDLVGLIFNTIGVSDGMANGTDGMRYSLVSRDLIA
                     DSIEAVCGAQYYDALIALPGCDKNMPGSVIAMGRLNRPSIMVYGGTIAPGHYKGQDLN
                     IISAFEALGQKIAGKLSDADFKGIVMNSCPGAGACGGMYTANTMAAAIEALGMSLPYS
                     SSNPALSEEKRQECLAAGAAIRVLMEKDLKPRDIMTRQAFENAITVIMVLGGSTNAVL
                     HLIAMARSVSVSLGQDDFQQISDRIPVLADFKPSGKYLMQDLHEHGGIPAVMKYLLEK
                     GLLHGHCLTVTGKTLAENLAAVPAIDFSKQDIILPLERPIKATGHLQILYGNLAEGGS
                     VAKISGKEGERFEGPARVFDGESPLIDGIHSGKIRPGDVVVIRYVGPKGAPGMPEMLK
                     PTSALIGAGLGKSVALITDGRFSGGTHGFVVGHITPEAHVGGAIALVHDDDIIELDAL
                     QNTITLKISDAELSRRKAAWKQPGLRATAGILFKYARQVKTAAEGCVTDEF"
     gene            complement(2817..>3518)
                     /locus_tag="VKU83_11505"
     CDS             complement(2817..>3518)
                     /locus_tag="VKU83_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLI94233.1"
                     /translation="RSAAALNMPYRYSTAEMIRATYEVLERNQQQDAYIRPVVYGPAN
                     MSFNPNTESFTVIQTWEMAPFLGEKMLRVMTSSFQRPNPKGFKIEAKAAGHYVNSIMA
                     SHEAKGKGFDEALLTDMNGYIAEGPGANMFFEKDGKLFTPAAGNILPGITRATVIELC
                     SELGIPVEEGQYTPAQLKAADAAFFCGTAAEVIGWASVDDYPFPLPWKESQGRRIQLA
                     YKNLVTERSHQLETI"
BASE COUNT          624 a         1009 c         1085 g          800 t
ORIGIN      
        1 gggccgtcag cagcggcagg gtttccttgc aatcgcccca gaccggcacc gcggatttga
       61 cattcttgtc gatctcggca gggtcgatgt cgaggtgaac gatcttcgcc tgcttcgcat
      121 acttgtcgag gcggcccgtc acgcggtcat cgaagcgcat cccgacggcg atcaggacat
      181 cgcagtcgtt ggtcaggacg ttaggcccgt agttgccgtg catgccaagc atgccgacgt
      241 tgagcgggtg gctcgtgggg agggcactga gtcccaggat ggtccaggct gcgggaattc
      301 ctcccttttc tataaaatcc cggaactcct gttcagcttt tcccaggatg acgccctggc
      361 cgaagacgac gaaaggccgt tccgcttcgt tgatgagctg cgccgcctta tcaacgaatt
      421 ccttcctgat gatcggcttt ggccggtaac tccggatatg ttcgcagcgg ctatatcccg
      481 caaagctgaa tttctgcagc tgggcgttct tggtgatatc gatcagcacg ggccccgggc
      541 ggccgctgcc cgcgatatag aaagctttgg cgagcaccga ggggatctcg gttgcgtcgg
      601 tcacctggta attccatttg gtaacaggcg tggtgatatt gatgacgtcg gtttcctgga
      661 aagcgtcggt accgagaaga tgggcgaaga cctggccggt gatgcagaca acgggcgtgc
      721 tgtcgatcat ggcgtccgca aggcccgtca ccaggttggt ggcgccggga ccgctcgttg
      781 caaagacgac tccggtcctg ccggaggtgc gggcgtagcc ctgtgccgca tgaacggcgc
      841 cttgttcgtg ccgcacgagg atatggttga gccggtcgcg gtaatcatag agcgcgtcat
      901 agatcggcat gatcgctccg ccgggataac cgaaaatggt gtcgacccct tcctccaaga
      961 aggcttctag gacggcggcc gacccgctca gctcgcgggg tgcggttggc tgcgctgcgg
     1021 actttctgtc gcccgctgtc gcggcggctt tcggtttgga cggagtggaa gtgagttggc
     1081 tcataaaact attttattca gttaacgttt gtcggcctgc ggaagcaaat cagaattcgt
     1141 cggtcacaca tccttcggct gctgtcttga cttgccgcgc atatttgaac aggatgccgg
     1201 ccgtcgcccg gagcccgggt tgtttccagg cggcctttct cctggaaagt tcggcatcgc
     1261 ttattttcaa cgtaatggta ttctgcaggg cgtcgagttc gatgatgtcg tcatcatgca
     1321 cgagggcgat ggcgccgccg acatgcgctt cgggggtgat atgcccgact acgaagccgt
     1381 gagtcccgcc gctgaagcgg ccatctgtga tcagcgccac gcttttcccc agacccgcgc
     1441 cgatcagggc agaggtgggt tttagcattt ccggcatccc gggcgcacct ttcggcccga
     1501 cgtaacggat gaccaccaca tcgcccggcc ggatcttgcc cgaatgaata ccgtcgatca
     1561 atggtgattc cccgtcgaag accctcgccg gtccctcgaa gcgctcccct tccttcccgc
     1621 tgatcttagc gacgctgccg ccttccgcaa ggttcccgta aaggatctgc aggtggccgg
     1681 tcgccttgat gggccgctcg aggggaagga tgatgtcctg ttttgaaaaa tcgatcgccg
     1741 gtacagcggc caggttctcc gccagcgttt tgccggttac tgtgaggcaa tggccatgga
     1801 gcagtccttt ttcgagcagg tatttcataa cggcgggaat gccgccgtgt tcatgcaggt
     1861 cctgcatgag atatttcccg ctcggcttga aatccgccag gaccggaatg cggtcgctga
     1921 tctgctgaaa atcgtcctgg ccgaggctaa cgcttacgct tctcgccatg gcgatcagat
     1981 gcagcacggc attggtgctg cccccgagga ccatgatcac ggtgatggcg ttctcaaagg
     2041 cctgccgggt catgatatcc cggggtttca ggtccttttc catcagcacg cgaatggcgg
     2101 cgccggcagc gaggcactcc tgccgctttt cttcgctcag cgcgggattc gatgacgaat
     2161 agggcaagct cattcccagc gcttcgatcg cggctgccat ggtgttggcg gtatacatgc
     2221 ccccgcacgc gccggcgccg gggcaggaat tcatcacgat gcctttgaag tcggcgtcgc
     2281 tgagcttgcc ggcaattttt tgaccgaggg cttcgaatgc cgagatgata ttgaggtcct
     2341 ggcccttgta atgacccggc gcaatggtgc caccgtagac catgatggac gggcggttca
     2401 ggcggcccat ggcgatcacg gagccgggca tattcttatc gcatcccggc agggcgatga
     2461 gcgcatcgta atattgggcg ccgcagacgg cttcgatgga atcggcgatg aggtcgcggc
     2521 tgacgaggga atagcgcatg ccgtcggtgc cgttcgccat gccgtcactg acgccgatcg
     2581 tgttgaagat gaggccgacg agatcttttt cccaaacgcc ttttttgacg agttgggcga
     2641 gatcattcag gtgcatgttg caggtgttgc cgtcgtagcc catggaggca atgccgacct
     2701 gcgctttggc aaggtccttg tcggtgagtc cgatgccata caacatggcc tgggcggccg
     2761 gttgcgtcac atcctgggtg agcgtcttgc tgtagtggtt caattccatg ctgcggttat
     2821 attgtttcca gttgatgaga ccgttcggtg acgagattct tgtaagccag ctggatcctc
     2881 cttccctggc tttccttcca aggcaacggg aacgggtagt cgtcgaccga tgcccagcca
     2941 atcacttccg ccgcggtacc gcaaaagaat gcggcgtccg cagccttcaa ctgcgctggg
     3001 gtatattggc cctcctcgac gggaatgccc agttccgagc agagctcgat caccgttgcc
     3061 cgggttatcc cgggcaggat attgccggcg gcaggagtga acagcttccc gtccttctcg
     3121 aagaacatgt tggcgcccgg cccctcggcg atatatccgt tcatatcggt caggagggct
     3181 tcgtcaaagc cctttccctt tgcttcgtgg ctggccatta tcgagttgac atagtggccc
     3241 gcggccttcg cttctatctt gaagcctttc ggattgggcc gctgaaaaga ggaggtcatc
     3301 acccgcagca tcttttcgcc gaggaatggc gccatttccc aggtctggat caccgtgaac
     3361 gactcggtgt tcggattgaa gctcatgttc gccggaccat aaacgacggg ccggatatac
     3421 gcgtcctgct gctggttccg ctcgagcacc tcataggttg cccggatcat ttcggcggtc
     3481 gaataccgat agggcatatt gagggctgcg gcggaccg
//
LOCUS       DATGKE010000680         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_253084, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000680 DATGKE010000000
VERSION     DATGKE010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253084"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VKU83_11510"
     CDS             complement(<1..431)
                     /locus_tag="VKU83_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94234.1"
                     /translation="MRKKNKNVTLAGVRVENYAAEGKSLARVEGKVIFIENAVPGDVV
                     DIRLSRNTKDWAEGMVTAFRELSAERIEPFCAHFGVCGGCRWQMLPYPKQLAYKQQQV
                     EDSLRRIGKIALPDIRPILGAPETVRYRNKLEYTFSNRRFLL"
     gene            459..1907
                     /locus_tag="VKU83_11515"
     CDS             459..1907
                     /locus_tag="VKU83_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HLI94235.1"
                     /translation="MSKILLSLAFSLLLMKAQAEEGYTIRLHLKPYTSGKVYLGYYYG
                     KIKALADSAQLNSNSDGIFSGSKALPGGVYFIVSPSRAILFELLIDSQQHFSVSADTT
                     NLPASVVFSGSPDNDVFQTYTRYTAEIGRQISADQQSLAAARKAHDSAAARIDQDKIR
                     ALNEALQKYREELARKYPTSLLATLFRSLKEPEIPPASKQPGGKYDSAYAFRYFKDHY
                     WDGISFSDERLSRTPVFEPRLDKYFRDLVSPAPDSIEKEVDRMLLEARVSKPMFQFLM
                     VYFVQKYVNPEYMGQDAVFIHLFDKYINAGETDFFTEKYRKYLNDRAYSMMANQIGEP
                     AANLEMVDSTGALRPLYGVNADYIIVCFWDPTCSHCQELVPRLDSMYKAKWAREGVKI
                     YGVMVDGGRQAWLQFIKDHNLTGWEHVYETKEHQDATEKAGQPGFRQLYDVYQTPTLY
                     LLDKDKRIIAKKLSLEQLDEVINLKLQHAKSK"
     gene            1891..>2759
                     /locus_tag="VKU83_11520"
     CDS             1891..>2759
                     /locus_tag="VKU83_11520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLI94236.1"
                     /translation="MQNPNDIRRLLSRIFLLNVLTLFLSSAVPAQTLFTIAGAPVSKE
                     EFLKAFAKNNNGITPTDKTYRDYLDLYIRYKLKVKAAYDAELDTLPAQRTELQNFRSQ
                     VADTYMKDQESLNKLIREAYERGQKDIRLSHILFSLPRDPSPMDTLRAYQRAMAAYTQ
                     LRKGKRFGETALAFSDDPSVKSNRGQIGYVTVFTLPYELETLAYSLAPGQYSKPCRTR
                     GGYHIFRNDGERKALGRVKVAQILLSFPPAATGAVREQVRATADSLYRVLQNGGDFAA
                     LAKVFSTDNLSYQ"
BASE COUNT          652 a          758 c          751 g          598 t
ORIGIN      
        1 agcaggaagc ggcggttgga aaaagtatat tcaagtttat tgcggtaccg gaccgtctcc
       61 ggggcgccga gaatgggccg gatatccgga agggctattt tgccgatccg ccggaggctg
      121 tcttctacct gctgctgctt atacgccaat tgtttcgggt agggcagcat ctgccagcgg
      181 caaccgccgc agaccccgaa atgcgcgcaa aatggttcta tccgctcggc ggagagctcg
      241 cgaaaagcgg tgaccatacc ctccgcccag tcctttgtgt tgcgggaaag ccggatgtcg
      301 acgacgtcgc ccggcactgc gttttcaatg aagatcactt taccttccac tctggcaagg
      361 gatttgccct cagcggcgta attctcgacc cggacgcccg ccagcgtgac gttcttattt
      421 ttttttcgca cggcgtaaag gtctaatttt acaggcgtat gagcaaaatc ctattgtcct
      481 tggctttcag cctgctcctc atgaaagccc aagcggaaga aggttacacg atccggttgc
      541 atttgaagcc ctatacaagc gggaaagttt acctgggata ttattacggc aagataaagg
      601 cgctggcgga ttcagcccag ctgaacagca acagcgatgg aattttcagc gggtcgaagg
      661 cgcttcccgg cggcgtgtat tttatcgttt cgccaagccg ggcaatcctg ttcgaattgt
      721 tgatcgactc acagcagcat ttctcggtat cagcggacac aaccaacctc cccgcgagcg
      781 tcgttttctc cggctcaccg gataatgatg tttttcagac ctacacccgg tacaccgctg
      841 aaatcggccg gcagatctcc gcggatcagc agtcgctggc cgctgcaagg aaagcgcatg
      901 attcggccgc ggcccggatc gatcaggata agatcagggc gttgaatgaa gccctccaga
      961 agtaccggga ggagctcgcc agaaaatatc cgacctcgct gctggcaaca cttttcaggt
     1021 cgctgaagga gcccgaaatt ccccccgctt cgaaacagcc gggcggaaaa tatgacagcg
     1081 catacgcatt tcgatacttc aaggaccatt actgggacgg gatctcgttc agcgatgagc
     1141 ggctgagccg gacgcccgtc tttgagcccc gtcttgataa atatttccgg gacctggtct
     1201 cacccgcgcc cgattcgatc gaaaaggagg tcgaccggat gctgctggaa gcgcgggtca
     1261 gcaagcccat gttccagttc ctgatggtct attttgtcca gaaatacgtg aaccccgagt
     1321 acatgggcca ggacgcggtc tttatccatc tgtttgacaa atacattaat gccggcgaga
     1381 ccgatttttt taccgagaaa taccggaagt atctgaacga cagagcctac agcatgatgg
     1441 ccaaccagat cggcgaaccg gcggcaaacc tcgaaatggt ggatagcacc ggcgcgctcc
     1501 ggcccttata tggagtgaac gccgactata tcatcgtttg cttctgggac ccgacctgca
     1561 gtcattgcca ggaacttgtc cccaggctgg attcgatgta taaagccaaa tgggcgcgcg
     1621 agggggtgaa gatctatggc gtgatggtgg acggaggaag gcaggcctgg cttcaattca
     1681 taaaggatca caatttgacc ggctgggaac acgtttatga gacgaaagag caccaggatg
     1741 caaccgaaaa ggccggtcag cccggatttc gtcagcttta cgatgtgtat caaaccccta
     1801 ctctgtatct gctggataaa gataagcgca tcattgcaaa gaagctgtct cttgagcaac
     1861 tggatgaagt tataaatttg aaactgcagc atgcaaaatc caaatgatat ccgccggctc
     1921 ctcagccgga tctttcttct caatgtactt acccttttcc tgtcttccgc tgtccctgcc
     1981 cagacgcttt ttacgatcgc cggcgcgccg gtcagcaagg aggagttttt aaaagcgttc
     2041 gcaaagaaca ataacgggat aacacccacc gacaaaacct accgggacta cctggacctc
     2101 tatatccggt acaagctgaa agtgaaagct gcctatgatg cggaactgga taccctgccg
     2161 gcgcaacgga ccgaattgca gaatttccgg tcccaggtcg cagacactta tatgaaagac
     2221 caggagagcc tgaataaatt gatccgggag gcttatgaaa gagggcagaa ggatatccgg
     2281 ctttcacata ttttattttc ccttccccgg gacccgagcc cgatggatac cctgcgggct
     2341 taccaacgtg cgatggcggc ctacacgcaa ctcagaaaag gaaagcgctt tggcgaaacg
     2401 gctctggcgt tttcggacga cccttcggta aagtccaacc gcggccagat agggtatgta
     2461 acggtattta cattgcccta tgagctcgag acccttgcgt attcgctggc ccccggccag
     2521 tactcgaagc cctgccggac caggggtggc taccatatct tccggaacga cggcgaacgc
     2581 aaggcgctgg gacgcgtcaa ggtggcgcag atcctgttat cctttccgcc ggcggcgacg
     2641 ggcgccgtcc gggaacaggt gcgcgccacg gcggattccc tgtaccgggt cttgcagaac
     2701 ggcggcgatt ttgccgccct ggcgaaagtc ttcagtaccg acaacctttc gtatcaaaa
//
LOCUS       DATGKE010000681         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_876578, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000681 DATGKE010000000
VERSION     DATGKE010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876578"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="VKU83_11525"
     CDS             complement(<1..2074)
                     /locus_tag="VKU83_11525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLI94237.1"
                     /translation="MHSNWKQTMELSKNYIPAATEDKWYEHWMSRRYFNSSPDNRPAF
                     TVVIPPPNVTGVLHMGHTLNETVQDILIRNARMRGYNACWVPGSDHASIATEAKVVGM
                     LKERGIDKNQLSRAEFMRHAYEWKEKYGGIIYSQIRKLGCSVDWERVAFTMDRDYYAA
                     VVSVFIDLYDKGLIYRGARMINWDPQAQTALSDEEVEYRDEMGKLYYVNYPLVDVAGA
                     RTGRHIMIATQRPETIMGDTAVCVNPADERYQDLAGAYALVPLVDRKVPVIFDEYVDP
                     GFGTGALKVTPAHDINDYNLGLKHGLPIVDTLNPDGTLSAAAEVFVGVERFSARKMVV
                     RQLREAGLMQKEEDHPTRVGYSQRTSAVVEPRISTQWFVKMAPLAQPALKAVTDGYIR
                     IHPGDKFLATYKYWLENVKDWCISRQLWWGQQIPAWYARDGSFRVARTREEAYARFAA
                     APDPGLVAGAATSPGSALPAAAPLPFTAADLRQDEDVLDTWFSSWLWPIEVFKGVTRP
                     ANPDIRYYYPTSVLVTGQDIIFFWVARMIMAGLYYFPDTNPMETRVPFHDVYFTGMVR
                     DKQGRKMSKSLGNSPDLLDLIDKYGADAVRFGIMIASPAGNDLLFDEAALEQGRNFNN
                     KMWNALKLVKGWEARLNPSSASGDPFPVNWMRSRIAEARGSIETLFNEFRLSEALKAL
                     YSLIWDDFC"
     gene            2772..>3070
                     /locus_tag="VKU83_11530"
     CDS             2772..>3070
                     /locus_tag="VKU83_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLI94238.1"
                     /translation="MLYAIVDIETTGGYAANNAITEVAIVLHDGNREVKRYETLVRPG
                     QSIPRYIQALTGITDEMVAEAPAFGEIAPVVHEWLKDAVFVAHNVNFDYSFLKHQ"
BASE COUNT          715 a          782 c          868 g          705 t
ORIGIN      
        1 agcagaaatc gtcccagata agagaataaa gcgccttcaa cgcttcactt agcctgaatt
       61 cattaaaaag tgtctcaatg gacccgcggg cttcagcgat gcgacttctc atccaattca
      121 ccgggaaagg gtcgcccgaa gcggacgatg gattgagccg tgcttcccag cctttgacca
      181 gcttcagcgc attccacatt ttgttgttga aattccggcc ctgctcgagc gcggcttcgt
      241 cgaagagcag gtcattgcct gccggcgagg cgatcatgat gccgaatcga acggcgtcgg
      301 cgccatattt gtctatgagg tcaaggaggt cgggtgagtt gccgaggctc ttgctcattt
      361 tccggccctg cttgtcgcgg accatgcccg taaaataaac gtcgtgaaag gggacgcggg
      421 tttccatcgg gttagtatcc gggaaataat acagcccggc catgatcatc cgggccaccc
      481 aaaagaagat aatatcctgg ccggtgacga ggacagaggt cggatagtag tatcggatgt
      541 cgggatttgc cggcctggtg acacctttga agacttcaat gggccacaac caggaagaga
      601 accacgtatc gaggacgtct tcatcctggc gaaggtcggc cgcggtgaaa ggcagcggag
      661 cggcggcggg gagcgcagaa ccgggagaag tggcggcgcc ggctaccagg ccggggtccg
      721 gggctgcggc aaatctcgca taggcctcct cacgggtccg ggcaacgcga aagctcccgt
      781 cacgcgcgta ccaggcaggg atctgctggc cccaccacag ctgccgggaa atgcaccagt
      841 ccttgacatt ttcgagccag tatttgtagg tagccaggaa cttgtccccc ggatgaatgc
      901 ggatataacc gtcggttacg gcctttaaag cgggttgggc gaggggcgcc atctttacaa
      961 accattgggt cgagatgcgg ggttcgacta ccgcgctggt tcgctgagaa taaccgacgc
     1021 gggtagggtg gtcctcctct ttttgcatca gtcccgcttc ccgcagctgc ctgaccacca
     1081 tcttccgggc cgaaaagcgt tccaccccga caaatacttc ggcggcggca ctaagggtac
     1141 cgtccgggtt gagcgtgtcg acgatcggca agccatgttt caggccaagg ttgtagtcat
     1201 taatatcgtg agcgggcgtg actttgagcg cacctgtgcc aaagccaggg tcgacgtact
     1261 catcgaagat aacaggcact tttctgtcga ccagcgggac gagggcataa gcgccggcca
     1321 gatcctggta gcgctcatct gcagggttga cgcagacggc ggtatcgccc atgatcgtct
     1381 ccggacgttg tgtagcgatc atgatgtggc ggccggtccg ggcgcccgcc acgtcaacga
     1441 gcgggtaatt gacataatag agtttaccca tctcgtcccg gtactcaacc tcctcgtcgc
     1501 tgagtgccgt ttgcgcctgc ggatcccagt tgatcatgcg tgccccgcga tagatcagac
     1561 ctttatcata taagtcgatg aaaaccgaaa cgactgcggc gtaatagtcc cggtccatgg
     1621 taaaggccac ccgctcccag tccacgctgc atcccagctt gcggatctgg gaatagataa
     1681 tgccgccgta tttttctttc cactcgtagg catggcgcat gaattccgcc cggctgagct
     1741 gattcttatc gatgcctctt tccttcagca tcccgactac cttggcctcg gtcgctatgg
     1801 aagcatggtc gctgccgggc acccagcagg cgttatagcc gcgcattctg gcgttgcgga
     1861 tgaggatatc ctggaccgtc tcattaaggg tatggcccat atgaaggacc ccggtcacat
     1921 tggggggcgg aatgaccacg gtgaaggccg ggcggttatc cgggctgctg ttgaagtacc
     1981 gccggctcat ccagtgttcg taccacttgt cctcggtagc ggcggggata tagttcttgc
     2041 tcaattccat ggtctgtttc caattggagt gcaaaaataa aaaaattccc cttgaagggg
     2101 aattcatata attcagtctg atattgtacg atagtggtgg ggatgagacc ggcttatgcg
     2161 aaccagtaca gccaggacaa gaacatcacc aggccaataa aacccagggc cagcaacgcc
     2221 gtctttgcag tctctccgat gtggatggag tgtaagaatt tcttcataat ggaatttttt
     2281 agggtgaaac caatagttga ttaaatagat taattggaat tcaaagggga tcggaaatca
     2341 acaattgcag aggtttctca gcgatctggg tgcggattag ggagggtaat tccgggttaa
     2401 aaataaaaca atttttcata ttatgaaaaa tctttcctca agggtagggt gggctgttgg
     2461 cctccaattg gaattatttg atactcattt atttgtgacg ccactggctt gaaaaactcc
     2521 agcgcctgag cgctggagtt agtaagtttc gtccggccgc tccactcacg ccatcgtgaa
     2581 caggtatgca gccgaaagga tggcgcagaa atacaaaact accgttacat agatcagtac
     2641 ggtgctttct gtgggtccga atgagtgcat tttttccatg gagattggga tttagtgaaa
     2701 actttgacta cggacaatat ttagcccgtg ttttcagggg tgggattaac agataaccga
     2761 tctttgtagc ggtgctatat gcgatcgtcg atatagaaac tacaggcggt tacgctgcta
     2821 ataacgctat aacagaagtt gctattgtgt tacacgatgg aaaccgggaa gttaagcgct
     2881 atgaaaccct cgtcaggcct ggccaatcca taccccggta tatccaggca ctaacgggaa
     2941 tcacggacga gatggtcgcg gaagcgccgg ccttcgggga aattgcaccg gtagtccacg
     3001 aatggctgaa agacgccgta tttgtagcgc acaatgtcaa tttcgactac tccttcctga
     3061 agcatcagtt
//
LOCUS       DATGKE010000682         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1291205, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000682 DATGKE010000000
VERSION     DATGKE010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291205"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..295
                     /locus_tag="VKU83_11535"
     CDS             98..295
                     /locus_tag="VKU83_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HLI94239.1"
                     /translation="MIEVTDKTLMPFGKYKGTALANVPAGYLIYIYENMNLHDNLKTY
                     IKKNMDVLKAEVARIRKNNAR"
     gene            318..1172
                     /locus_tag="VKU83_11540"
     CDS             318..1172
                     /locus_tag="VKU83_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94240.1"
                     /translation="MSVKVPGFIFYPGDYLQDTQNLSADAQTAYDRIMCLHMKNICIS
                     KQQLDFFTKRLTAEQKSELLMVLVVNGEGEESTYRIAWVTDSILKYRKFCESRSSNRK
                     GKNKEQVKNTSSTSEQLVENESVNEDEIENEFKEGGEGEGSQTGYANDRLLVPQMLQK
                     WKEALPNYPDDPILDGKALYSIAIFLCKRGNLRGAPELNVEPILEAWEQLCAFISEDR
                     FYKQKSLKTISNGIQEILQKALHGDQSKTTPVDGKGSGKFNDDKLKAGIRARVAERER
                     AQGQTGTG"
     gene            1201..1716
                     /locus_tag="VKU83_11545"
     CDS             1201..1716
                     /locus_tag="VKU83_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94241.1"
                     /translation="MRKVLEVPLNERIPALLEHPENYEPIYTALVASLKSALTNIHVR
                     VGMGDDQVLELAQMILDSAKEDNLGLEDVLLFLGELLHGKAGKIYDRLDIPTFFELFE
                     YYRQRRHEMMKDFREEQSAQFKALPVDIRFVHENTEAEKTAIRRAATEYFINTSKPIS
                     DGMDGAKTEQP"
     gene            1682..1915
                     /locus_tag="VKU83_11550"
     CDS             1682..1915
                     /locus_tag="VKU83_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94242.1"
                     /translation="MAWTAQKLNNLKNHFALTDKQIGTAIQERTFMVWKMRNGVIDLA
                     KYESRLSGYLKALKAAKRAEMDAMADFFLSFED"
     gene            1960..2184
                     /locus_tag="VKU83_11555"
     CDS             1960..2184
                     /locus_tag="VKU83_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94243.1"
                     /translation="MAQQIGAAQMRKLGKKIRNEIPVNIGFCLLIFEFHKPGIANYIS
                     NAQREDMIKSLRETADRLEKGQDFKTPEEN"
     gene            2189..2590
                     /locus_tag="VKU83_11560"
     CDS             2189..2590
                     /locus_tag="VKU83_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI94244.1"
                     /translation="MTLSGIQLIEDMAQENPDYTIRDYPDIVEEIENIKTEDMGSRKG
                     IPNLTDDKMNQIIQLAKEKKSVPEICAIIGISQNCVYKACSRKGPGYKALSCPQVIEP
                     DPVVKEPEKKSEKWRRPPAEYSNHSPYGIAS"
     gene            2832..3068
                     /locus_tag="VKU83_11565"
     CDS             2832..3068
                     /locus_tag="VKU83_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94245.1"
                     /translation="MRRHLSMSVDGALKMKRKLHHLLQDETGKLLTDKEAREHLRECQ
                     ARGIKKIMVGDCDNFDPIKGCMGHPSDNQQTQPS"
     gene            3065..3544
                     /locus_tag="VKU83_11570"
     CDS             3065..3544
                     /locus_tag="VKU83_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HLI94246.1"
                     /translation="MNRSIQPGSGAEFSEDRKYRYALWRIWNPDKPLVMFIGLNPSTA
                     NERTNDATIKRVMSITGYWGCGGFYMMNLFSIVSRDPDLLINHPDPLGENDLWLDKIA
                     IDCERIVFAWGAFKQAKERAEVVAKRFPGAYCLRKTKDGHPWHPLLIPIKTQPIPFK"
     gene            3552..3887
                     /locus_tag="VKU83_11575"
     CDS             3552..3887
                     /locus_tag="VKU83_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94247.1"
                     /translation="MNTPNLSTSADKDEEITALKKALEEKSEEIKRLRNMIPKWFSTK
                     DMFPEKTGRQAYEHVDCLVLKGGYIERLMWNCEEKCWDDAEGDDYECDAEAVSHWLPM
                     SLIATLEKP"
     gene            3914..4168
                     /locus_tag="VKU83_11580"
     CDS             3914..4168
                     /locus_tag="VKU83_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94248.1"
                     /translation="MKSATIFKKLREYLKPEHVGRLELMHSLAPELAMVLTMADVIRE
                     YNSTEEDEKEVSLLLEHLAKNHFSTPKKSPPPAIPWKRKK"
     gene            4150..4542
                     /locus_tag="VKU83_11585"
     CDS             4150..4542
                     /locus_tag="VKU83_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94249.1"
                     /translation="MEEKEIIEGTKAICLFIGGEIREAWRVQETVHYTWYGEKVNDWR
                     KIGGMPILEGKYAMCILLEHCKWHSDWNWLMPLVNAIRKMLNRDADNYRDMRNRWQPI
                     ANELQNCDLNNVWFCVVKFIEWYNTQQH"
BASE COUNT         1528 a         1047 c         1148 g         1012 t
ORIGIN      
        1 ccgggagcag gttaagcagt gtggcaatgc cgtaaccccc ccggcaatgg aatggctggc
       61 gggacaggta gtgaaatcat ttgaaagata ataacgtatg atagaagtaa ccgacaaaac
      121 cctgatgccc tttggcaaat acaaaggcac ggccttggcg aatgtgccgg ctggctacct
      181 gatctacatc tatgagaaca tgaacctgca cgacaaccta aagacgtaca tcaagaagaa
      241 tatggatgta ctgaaggcgg aggtggccag aataagaaaa aacaacgccc gataagttca
      301 ctcgctaaaa tcccaatatg tccgtcaaag taccaggctt tattttctac ccaggtgatt
      361 atttacagga tacgcaaaac ctaagcgcag acgcgcaaac ggcttacgat aggatcatgt
      421 gtttacatat gaagaacata tgtatttcta aacaacaact agacttcttc accaaacgtc
      481 tcacggcgga gcaaaaatct gaactgttga tggtgttggt ggtgaacggc gagggcgagg
      541 aatctaccta ccgaattgca tgggttacag atagtatatt gaaatacaga aagttttgcg
      601 agtccagaag cagtaacagg aaaggtaaaa acaaggaaca agtgaaaaac acatcttcta
      661 caagtgaaca actagtggaa aatgaaagtg taaatgaaga tgaaattgaa aatgaattta
      721 aggaaggggg tgagggggaa ggaagccaga ccggttacgc caatgatcga ttgttggttc
      781 cgcagatgct gcagaagtgg aaagaggcac tgcctaacta ccccgacgat cctattttgg
      841 acggtaaggc actctatagt atagctattt tcctgtgcaa acgcggcaat cttcgcggag
      901 cgcctgagtt gaatgtagag cccattctgg aagcatggga acaactctgc gcattcattt
      961 ctgaggaccg gttctacaaa caaaaatcgc tgaaaaccat ttccaacggc attcaagaaa
     1021 tacttcaaaa agcactccat ggagatcaat caaaaacaac tcccgtcgac gggaaaggct
     1081 caggaaagtt caatgacgac aaactcaaag ccggtattcg cgctcgtgtt gctgagcggg
     1141 aacgcgcaca gggtcaaaca ggaactggct aggtatcaga ctccatccgg gatgacagac
     1201 atgcgcaagg tcttggaagt gccgctgaat gaacgtatcc cggcccttct ggagcatcca
     1261 gaaaactatg agccgatcta tacggcttta gtagcctctc ttaaatctgc cctcacaaat
     1321 atccacgtca gagtgggtat gggcgatgac caggtgctgg aactcgccca gatgatcctg
     1381 gacagcgcca aggaagacaa ccttggactg gaggatgtgt tgctattcct tggtgagtta
     1441 ttgcacggca aagctggtaa gatctacgac agactggata taccgacctt ttttgaattg
     1501 ttcgaatact accggcaacg gcgccatgaa atgatgaaag atttcagaga ggaacaaagc
     1561 gcccaattta aagccctccc cgtggatata cgattcgtcc atgaaaatac tgaagcagaa
     1621 aagacggcca tcagaagggc cgctacggaa tattttatca atacatccaa acctataagc
     1681 gatggcatgg acggcgcaaa aactgaacaa ccttaaaaac cattttgccc tgaccgataa
     1741 gcaaatagga acggccatcc aagagcgcac cttcatggtc tggaaaatgc gcaatggggt
     1801 catagatctc gctaaatatg aaagccgcct atccggctat ctcaaggctt taaaagcagc
     1861 gaaacgggca gagatggacg ccatggcaga tttcttttta tcctttgagg attgaacctt
     1921 tttaacccag gccctccggg gcctataaaa tagaatgaaa tggcgcaaca aataggagcg
     1981 gcccagatgc gcaaactggg caaaaagatt agaaatgaaa taccggtgaa catcggcttt
     2041 tgcctgctga tatttgaatt tcacaagccg ggaatagcca actatatatc caacgcccaa
     2101 agggaggata tgataaaatc actgcgagag acagcggata gattagaaaa aggccaggat
     2161 tttaaaacac ctgaagagaa ttagccccat gaccctctcc ggcatacagc tcatagagga
     2221 tatggcccaa gagaatccag actataccat ccgggattac ccggatatag tggaagagat
     2281 tgaaaacatc aaaaccgaag atatgggaag cagaaaaggc ataccgaacc tgacggatga
     2341 caaaatgaac cagatcatcc aactggccaa agaaaagaaa agcgtacccg agatatgcgc
     2401 gatcatcggg atatcccaga attgcgtcta taaggcttgc agcaggaaag ggccgggtta
     2461 taaggcatta agttgtccgc aggtcatcga gccggatcct gtcgttaaag agccggaaaa
     2521 gaagtcggag aagtggcgca gaccaccggc ggagtattcg aatcattcac cttatggaat
     2581 agcatcatga tacaccaagc agataaggaa ctaaaacaac gatggctggc cgctttcaat
     2641 ggccttgagc cgtctgccaa agctgccctg aaactttgcc ttctatccct gcgagaggat
     2701 gccctacata aggcaaatta ttgctggaag aaacacaagg ctccgatggc cctttactgg
     2761 aaagtggtgg gcgtatatgc cggtcacctt gccagagcaa taaaattgac aacagataaa
     2821 cctgagacgt tatgaggcga cacctatcca tgagcgtaga cggtgctctg aaaatgaaga
     2881 ggaaactgca ccatcttttg caggatgaga ccggcaagct gttgaccgac aaagaagctc
     2941 gtgaacatct tcgggaatgc caggcccggg gcatcaaaaa gatcatggta ggagactgcg
     3001 acaacttcga ccctataaag ggctgtatgg gccacccatc tgataaccaa caaacccaac
     3061 catcatgaat agatccatcc agccgggaag cggcgctgaa tttagcgagg acagaaaata
     3121 ccgatacgcc ctatggcgca tctggaaccc ggacaagccc ctggttatgt ttatcgggct
     3181 taatccgtcg acagccaacg agcgcacaaa tgacgccacc ataaagcggg taatgagtat
     3241 taccggttat tggggatgtg gcggattcta tatgatgaac cttttttcca tcgtcagccg
     3301 tgatcctgac ctgctgatca atcacccgga ccccctaggc gaaaatgacc tatggctgga
     3361 taaaatagcc atcgactgcg aaaggatcgt ctttgcgtgg ggcgcgttca agcaggcaaa
     3421 ggagagagcg gaagttgttg caaaacgatt tccgggggcc tattgccttc gaaagacaaa
     3481 agacgggcat ccttggcatc cgttgttaat ccctatcaaa acgcaaccta taccatttaa
     3541 ataaacgcat catgaacacc cccaatctat ccacctccgc cgataaagat gaagagataa
     3601 ccgccttaaa gaaagctctt gaagaaaagt cggaagagat taaaaggctc agaaatatga
     3661 ttccgaagtg gttcagcacc aaggatatgt tcccagaaaa aacggggcga caagcatatg
     3721 aacatgtcga ttgccttgta ttaaaaggag gctatatcga acgtcttatg tggaattgcg
     3781 aagaaaagtg ttgggatgat gctgaaggcg acgattatga atgtgatgcc gaggccgtct
     3841 cccattggtt gcccatgtca ttaattgcta ccctcgaaaa accttaaccc atccacccag
     3901 taaataaaca gccatgaaat cagccacgat atttaagaag ctcagagaat acctgaagcc
     3961 ggagcatgta ggacggttgg aacttatgca ttcattggca cccgaattgg ctatggtttt
     4021 gacaatggct gatgtaatcc gggaatacaa ttccacggaa gaggacgaaa aggaagtatc
     4081 gcttctccta gagcatttgg ctaaaaatca tttcagtaca cccaaaaaat cccccccccc
     4141 agcaatacca tggaagagaa agaaataata gaaggaacca aggctatctg cctttttata
     4201 ggcggggaga taagggaagc ttggcgtgtt caggaaacag ttcattatac ctggtatgga
     4261 gaaaaagtaa atgactggcg gaaaattggc ggtatgccta tactcgaagg caaatacgcc
     4321 atgtgcattc ttttagaaca ttgcaaatgg cattcggatt ggaattggct tatgcctctc
     4381 gtaaacgcaa tccgcaaaat gctgaatagg gatgcggata actacagaga catgagaaac
     4441 aggtggcagc ctatcgccaa tgagcttcag aactgtgacc taaacaatgt ttggttttgc
     4501 gtggtgaagt ttatcgaatg gtacaacacc caacaacact aataaaacac ctatacaatg
     4561 agtaaggaac caaaatttac aaaaaaagca aaaggaggca atttatgacg ctatgtatga
     4621 ttatgctcag ccccttgttg aagctcttga ccgggcaatt tctgttattg agaccgagtg
     4681 tgacgatgat ctactcatta aagaactaaa ggaggtagtt gccgaatata aacct
//
LOCUS       DATGKE010000683         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_813842, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000683 DATGKE010000000
VERSION     DATGKE010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813842"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VKU83_11590"
     CDS             complement(<1..365)
                     /locus_tag="VKU83_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HLI94250.1"
                     /translation="MADYIRCPNWFHRLPSIYKLVMSFAAATIVSVALLPVHMERMTR
                     VMIGWDVFSICMLMISAVIFSSMRPRQIRLLAKQEDAGRLVVFGIVVAATIGSLMGVL
                     ILLGKKAFLLGKGVESFIY"
     gene            403..2019
                     /locus_tag="VKU83_11595"
     CDS             403..2019
                     /locus_tag="VKU83_11595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLI94251.1"
                     /translation="MQPFSRLAGSSILVTLFFLLVFPSEISAQLYNPVIDVQHYDYSL
                     ALNDNNDNIRGTAVVTVRFRTAASQFVLDLRAKDSTGKGMTVLSATENNAPVRFTQDS
                     THLTLKSGGGPGTQHSYTIRYEGIPADGLVIAKNKYGHRGFFGDNWPNRAHNWLPCVD
                     DPSDKATLDWRITAPEHYQVVANGALQEEHTYSNHFKLTHWKESAPLAPKIMVIGVAD
                     FAIDHPGDAMGVPVWNYVFPEDKETGFHAYSCAMQILPFYITHIGLYPFEKCGNVQSK
                     TRFGGLENASAIFYFENSVNSPGIESLMAHEIAHQWFGDCATESRWQHLWLSEGFATY
                     MTHCYLESRYGPDTLKKGMERDRRTTIEYEKSRFTPVVDSTVQGDYMQLLNPNSYEKG
                     GWVLHMLRRRLGDSLFWKGLSAYYNTYKNRNAGTGDFEKVMETVSRRNLDTFFQQWLY
                     TPGHPNLEIAWKYDADKKALLLDITQTQQQLFDFPFEYSVDGGLYHADVRGKVTHVEQ
                     PLPAPPARFIPDPNVNLLASFKISGPHEAP"
BASE COUNT          503 a          673 c          529 g          451 t
ORIGIN      
        1 atatagataa agctctccac cccctttccc agcaggaatg ctttcttgcc tagtaagatc
       61 agcacgccca tcaggctgcc gatcgtcgct gccacaacga tgccgaagac taccagccgg
      121 cctgcgtcct cctgcttcgc cagcaaccgg atctgacggg gccgcatgct cgaaaagatg
      181 accgcactga tcatcagcat gcagatgctg aaaacgtccc agccgatcat cacccttgtc
      241 atgcgctcca tgtgcacggg tagcagggca acagacacga tcgtcgcggc tgcaaaactc
      301 atcaccagct tgtagatcga cggtaatcgg tggaaccagt tgggacacct gatgtaatct
      361 gccatactgc aatttaaagt atatttatcc caaattcttt ccatgcagcc attttcgcgc
      421 ctggccggtt cctccatcct cgtgacgctt ttctttttgc tcgtttttcc ttccgaaatt
      481 tccgctcaat tatacaatcc ggtgatcgat gtgcagcatt acgactactc gctcgccctc
      541 aatgataaca atgacaatat ccgcgggact gctgtggtaa cggtccggtt ccgaaccgca
      601 gcctcacagt tcgtacttga cctcagggcc aaagacagca ccggcaaagg catgaccgtg
      661 ctctcggcaa cggaaaacaa cgcgcccgtc cgcttcaccc aggatagcac gcacctcacg
      721 ctgaaaagcg ggggcggccc gggcacgcaa cacagctata ccatccgcta tgagggtatt
      781 ccggccgacg gcctcgtcat cgccaaaaat aaatacggcc atcgcggatt cttcggtgat
      841 aactggccga accgcgccca caattggctg ccctgcgtcg acgacccgtc ggataaggcc
      901 acgcttgact ggcggatcac cgccccggaa cactaccagg tggtcgccaa cggggcactc
      961 caggaagagc atacttacag taatcatttt aaactaaccc actggaagga atctgccccg
     1021 ctcgccccca agatcatggt catcggcgtc gccgactttg ccatcgacca cccgggtgac
     1081 gccatgggcg tgcctgtctg gaattatgtc ttcccggaag acaaagagac cggattccac
     1141 gcctattcct gcgctatgca gatccttcct ttttacatca cccatatcgg cctctatcct
     1201 tttgaaaaat gcggaaacgt tcagtcgaag acccggtttg gcggcctgga gaatgccagt
     1261 gccattttct atttcgaaaa ctcggtcaac tctccaggta ttgagtcgtt gatggcgcac
     1321 gagatcgccc accaatggtt cggcgactgc gcgaccgaat ccaggtggca gcatctgtgg
     1381 cttagtgaag gttttgccac ttatatgacc cattgctacc tcgagagccg gtacggcccg
     1441 gacaccttga agaaaggaat ggaaagggac cgccggacta ccatcgaata tgaaaagagt
     1501 agatttacgc cggtcgtgga cagcaccgtc caaggcgatt atatgcagtt gctcaatcca
     1561 aatagttatg aaaagggcgg ctgggtgctg cacatgctcc ggcgccgcct gggcgacagc
     1621 ctcttctgga aaggcttgtc cgcctattac aatacctaca agaaccgcaa cgccggcacc
     1681 ggggatttcg agaaggtgat ggaaaccgtc agccgccgga atctcgatac cttttttcag
     1741 cagtggctgt acacaccggg ccaccccaac ctggaaatcg cgtggaagta cgatgcggac
     1801 aagaaagccc ttctcctcga cattacacaa acccagcagc agctattcga tttcccattc
     1861 gaatattccg tcgacggcgg cctttatcat gcagacgtcc ggggcaaggt aacgcacgtc
     1921 gagcagccgc tgccagcgcc gcccgcccgc ttcatccccg atccaaacgt caacctcctg
     1981 gcgagcttta aaatttcggg gcctcatgaa gcgccctgat tcaaccgacc actttcgcgc
     2041 actcgttggc gcaggacctt cactcggttc tgaggctctc tgcggggttc accctgacgg
     2101 cacgcaaccc ctgccagcca accgttagaa aactgatccc cactgcaagc gccgcg
//
LOCUS       DATGKE010000684         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1496954, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000684 DATGKE010000000
VERSION     DATGKE010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496954"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VKU83_11600"
     CDS             <1..1329
                     /locus_tag="VKU83_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HLI94252.1"
                     /translation="PLWVFGDIASDDAAVGNTGATPDGATIDNFSFSTTNSHLSNEWA
                     NFYKGISDCNLVLANVPGINMDTAMRSRILGEAKFLRAWYYFMLVNIYGDVPVVVTPL
                     TPAQMQIAQSPEKLIYETVIEPDLMAALAALPASYTGADVGRATSGAAASLLAKVYLY
                     QEKWDSSLTYSNLVINSGQYGLMNVYSHDFDALHKNNRESIFEVQMLGGQNPNVGDAL
                     NQWFAPQVVGGYYCDAPNENFVAEFEETSQGVYDPRLDYTVGRDSMLWNNGQVYLSSW
                     SPQTGYLTRKYQQPLSQAPIIGQGSCDYVAIRYADVLLFNAEALNELGQTSQAVIPLN
                     EVRKRARESYLYDSTLNGYGSVPPGLLPDVAAVSQSQVRTAIIHERRVELGFEFHRYF
                     DLMRYGANPALGPSYIEPLINRDGVTAFSYTKDKTFPIPQSERDLDKALQ"
     gene            1362..2447
                     /locus_tag="VKU83_11605"
     CDS             1362..2447
                     /locus_tag="VKU83_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HLI94253.1"
                     /translation="MRPKARINQLAIAITLGLSLAFVIFGCKKSSHFTPPDTSSLTAA
                     IDSAEWYLTNTHEGTQPGEYLAGSQAALKSALQSAQAVLSSASTTATQAQVTAATANL
                     NAAIAAYEGAKVVPVDAASIVAYWKFNGNANDSSGKGHNGTLEAGPTGLAAVPGPVPN
                     LVNDRFGNANSAYHFSGGGNIDVPYSPALNPTAMSISLWCRQDTAGRTDHPNDCYMIS
                     LSRWNGWKFQTQPSRPFLTVSTDTSIYDRDAGTAMTIGTTTGAGPWNHLVATYDGNGT
                     ESFYINGVLVKTWTNLAGKIKPINPTYDLAIGSDLVNSAYSPSMSDPNYIAWGGYWTG
                     DLDDIIIYNVALTASQVTQIYNQQASQ"
     gene            2516..3817
                     /locus_tag="VKU83_11610"
     CDS             2516..3817
                     /locus_tag="VKU83_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLI94254.1"
                     /translation="MRICILILAFFVVESTLPSCRNNASGGKNNILFWCSNNNQEIQF
                     CTHITRIWNDSHRFGDVHLQPVPEGQSSEEVILAAVVGKTTPDIYANMWQGSVEMYAH
                     AGVLVALDTLKGFMAFIKQRCDSAAIREITSSNGHIYQIPWKVNPIMTLYNKTLFERN
                     QINQLPGTYSGYYKAAEAFKDNAARNGIPGSRFGYTEVKSIWYQRLFNFYTLYVAASD
                     GAPLIVNNRAAFNNKYAVEVFAFLQHLYNKEYFSKESTSASADPFVMQRIATEWTGPW
                     EISYLNNIPGKGFDFDYYPPIVPDDHLGPIFTYADPKNIVIFKSCRNPQLAWAFLETL
                     LDRKGDLELLTTTGQLPRRKNLETDPFFSEYFRANPMMVRFAEEIPYLKGVDNCDVIV
                     EVLDIISQEYEACVVYGMKTPERAVADAEKAVNVLLASANR"
     gene            3819..>4803
                     /locus_tag="VKU83_11615"
     CDS             3819..>4803
                     /locus_tag="VKU83_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLI94255.1"
                     /translation="MTSELRIGILGAGGFAAFAANAFSEISGVAIHSVFDVYRPAAER
                     LAAGLHAAVPENIDAFLADSRLDLVYIATPPFLHYEQSRLALLAGKHVICEKPAALRT
                     AEAEELRSISRANRLLWVVNLMQRYNPLYATVKQITDNQLMGAFLHGFFENYASDENL
                     AASHWFWDAVKSGGIFIEHGVHFFDMFAGWLGEGRLASSLQLRRPGISSGIIDRVHAV
                     VVYKDSPVQFYHGFDQPALLDRQECRLLFERGDIRLDGWVPVRLTAHGLLGNNELRVL
                     RDLLPGASVHATVAKSSGHKPRGRFKDIDYDEEVTIEYGNIGDKQPRYRDIL"
BASE COUNT         1182 a         1286 c         1271 g         1064 t
ORIGIN      
        1 cctctttggg tatttggtga tatcgcatcg gacgacgcgg ccgttgggaa cacaggagcg
       61 actcccgatg gcgcaaccat cgataatttt tccttctcaa caactaattc acacttgtcg
      121 aatgagtggg caaacttcta caaagggatt agcgattgca atctggtcct ggccaatgtg
      181 ccgggcatca atatggatac cgccatgcgc tcgcgaatcc tgggagaggc aaagttcctg
      241 agagcctggt actatttcat gctggtaaat atctatggcg acgttccggt cgtcgttacc
      301 ccgctcacgc cggcccaaat gcaaatcgcc caatcacccg aaaaactgat ctatgaaacc
      361 gttatcgagc cggatctgat ggcagcactt gccgcgttgc ccgccagcta tacgggcgcg
      421 gacgtgggca gggcaaccag cggcgccgcc gcatcgttac tggctaaagt ctacttgtac
      481 caggaaaaat gggattcatc gctaacctat tccaaccttg tcatcaacag cggccagtac
      541 ggcttaatga atgtttattc gcatgacttc gacgcgcttc ataagaacaa tcgcgagtcc
      601 atatttgaag tgcaaatgct tggcggccag aaccccaatg tcggcgatgc gctgaatcag
      661 tggttcgcgc cccaggtagt cggggggtac tactgtgacg cgccaaacga aaattttgta
      721 gccgagttcg aggagacttc ccagggggtt tatgatccga gactggatta tacggtcggg
      781 agggactcca tgttatggaa taacggtcag gtctatctaa gttcatggtc gccgcagacc
      841 ggatacctga caagaaagta tcaacaacct ctttcacagg cccctatcat cggacagggc
      901 agttgcgatt acgtcgccat aaggtacgcc gatgttcttt tgttcaatgc ggaagctcta
      961 aatgagctgg gtcagacatc ccaggcggtt attcccctta atgaggttag aaagagagca
     1021 agggaaagct acctgtacga cagcaccctg aacggatacg gttctgtgcc gccggggctt
     1081 ctgccggacg tggccgcggt gtcgcaatcg caggtaagga cggccatcat ccatgaaaga
     1141 agggtagagc tcggctttga gtttcaccgc tacttcgacc tcatgcgcta tggcgccaac
     1201 cccgcccttg ggccctccta catcgagccg ctgatcaata gggatggcgt aacggccttt
     1261 tcctatacga aagataaaac cttcccgata ccacaaagtg aaagggatct cgacaaagct
     1321 cttcaataac aagaaattca tcaattgtaa aattcaccaa tatgagacca aaagctcgca
     1381 ttaaccagtt ggcgatcgcc attacgctag gactgtcatt ggctttcgtt atattcggct
     1441 gcaagaaaag cagccatttc acgccgcctg acaccagcag cctgacggca gcaatcgatt
     1501 ctgcggaatg gtatctgacg aatacccacg aagggacgca gcccggggaa tatctcgccg
     1561 gttcccaggc agcactgaaa agcgcattgc agagtgccca ggcagtgctg tcgagcgcaa
     1621 gcacaacggc gacccaggcg caggtgactg ccgctacagc caacctgaat gcggcgattg
     1681 cagcgtatga aggtgcaaag gtcgtcccgg ttgatgcggc gtcgatcgtc gcttactgga
     1741 aattcaatgg aaatgccaac gactcttccg gtaaaggcca caacggaacg ttggaagcag
     1801 gtcccaccgg cctcgcggcg gtgcctggtc ccgtcccgaa cctggtgaac gaccgtttcg
     1861 gaaatgccaa ctcagcctat cacttctccg gcggcggcaa tatcgatgtg ccgtatagtc
     1921 ccgccctgaa tcccaccgca atgagcatca gcctgtggtg ccgccaggac accgcaggaa
     1981 ggaccgacca ccccaacgat tgctacatga tttcgctaag ccgttggaac gggtggaaat
     2041 tccaaaccca accatcccgg ccattcctga ccgtttcgac cgacacttcg atctatgacc
     2101 gcgacgcggg aaccgcaatg acgatcggta ccaccaccgg cgcgggaccg tggaaccacc
     2161 tggtagccac ctatgatggc aacggcaccg aatcctttta tattaatggc gtcctcgtca
     2221 agacctggac gaacctggcg ggtaagatca agccgatcaa ccccacttac gatttggcga
     2281 taggatcgga ccttgtcaac agtgcctatt ctccctcgat gtcggacccc aattatatcg
     2341 catggggggg ttattggacc ggtgatctgg acgatatcat catttacaat gtcgctttga
     2401 ccgcatcgca agtcacgcag atatataatc agcaggcaag ccaataaggc gccgcgcttt
     2461 ttcctgaaag accactacgg atcatataga ccgtagtggt tttttaaacc cttctatgag
     2521 aatttgcatc ctcatacttg ctttttttgt cgttgaatcc accctgcctt cctgcaggaa
     2581 caatgcatcc gggggtaaaa acaatatatt attctggtgc tccaataaca accaggaaat
     2641 acaattctgc acccatatca caaggatctg gaatgacagc caccggtttg gcgacgtcca
     2701 tctgcaacca gtaccggaag gccaatcaag cgaagaggtc atcctcgctg cggtggtcgg
     2761 caagaccact ccggatatct atgccaatat gtggcagggc agcgtagaaa tgtatgcaca
     2821 cgccggcgtg ttggtcgcat tggatacgtt gaaaggtttt atggccttca tcaaacaacg
     2881 atgcgacagt gccgctatca gggagatcac gtcatcaaat ggccacatct accagatccc
     2941 ctggaaggta aatcccatta tgaccctgta caataaaacg ctctttgaaa gaaatcagat
     3001 caatcagctt cccggaacgt atagcggata ctacaaggca gccgaggcgt tcaaggataa
     3061 cgctgccagg aatggaatac cgggcagcag gtttggctat accgaggtaa aatcaatatg
     3121 gtatcagcgc ctcttcaatt tttacacgtt gtatgtcgca gcctcagacg gcgcgcccct
     3181 gatcgtcaat aaccgggcgg cgttcaacaa taaatacgcc gtggaagtct ttgccttcct
     3241 gcagcacctg tataacaaag agtatttttc aaaagaatcg acttccgctt ccgccgatcc
     3301 gtttgttatg caacgcatcg ccacggagtg gacgggaccg tgggagatat cttacctgaa
     3361 taatatcccg ggcaagggat ttgacttcga ttattaccct ccgatcgtcc cggacgatca
     3421 cctgggtccg attttcacct atgccgatcc aaaaaatatc gtcattttca agagctgcag
     3481 aaacccgcag cttgcctggg cgttcctgga aacgttgttg gacagaaaag gcgacctgga
     3541 actgctgacc acgacgggtc aattgcccag gaggaaaaac ctggaaaccg atccattctt
     3601 ttctgaatat tttcgcgcga acccgatgat ggtgcggttc gctgaggaaa ttccctatct
     3661 gaagggcgtt gacaactgcg acgtgatcgt agaggtcctt gatatcatct cgcaggaata
     3721 tgaggcctgc gtggtctatg ggatgaaaac tccggaaaga gccgttgcag acgctgaaaa
     3781 agcggtgaat gtcctgttag ccagtgcaaa ccgataaaat gacaagcgaa ttacgcatag
     3841 gaatcctcgg cgccggcggc tttgcagcat tcgccgcgaa cgctttttcc gagatcagtg
     3901 gcgtcgcgat ccattccgta ttcgatgtct accggcccgc ggctgaaagg ctggcggcag
     3961 gcctgcacgc ggccgtgccg gaaaacatcg atgcattcct ggcggacagc aggctggacc
     4021 tcgtgtacat tgctacgccg cctttccttc actatgagca atccaggctg gcgttgctgg
     4081 ccggcaaaca tgtgatctgc gaaaaacccg cggccttgcg cacggccgag gcggaagaac
     4141 ttcggtcgat ttcccgggct aaccggctac tgtgggtggt gaaccttatg cagcggtaca
     4201 acccattgta tgccacggtt aaacagatca cggacaatca attgatgggc gcctttttgc
     4261 atgggttctt cgaaaattac gccagcgatg aaaacctggc ggcgtcccac tggttttggg
     4321 acgcagttaa atcggggggg atatttatcg agcatggcgt tcacttcttc gacatgttcg
     4381 cgggttggtt gggggaaggc cggttggcaa gctccctgca actgcgccgt ccgggtattt
     4441 catccgggat catcgacagg gtccatgccg tcgtcgtgta caaagacagt ccggtacagt
     4501 tttatcatgg gttcgaccag cccgcattgc ttgaccgtca ggaatgcagg ttgctttttg
     4561 aacgggggga catccgtttg gatggctggg tcccggtccg gctgaccgcg catgggctgc
     4621 tgggaaacaa tgagctgagg gtcttaaggg atctcctgcc aggggcatcc gttcatgcca
     4681 ccgttgcgaa aagctccggg cacaagccca ggggccgctt taaggacatt gattacgatg
     4741 aagaagtcac cattgagtac ggaaatatcg gggataagca gccgcgatac cgggacatat
     4801 tga
//
LOCUS       DATGKE010000685         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_953742, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000685 DATGKE010000000
VERSION     DATGKE010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953742"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1115
                     /locus_tag="VKU83_11620"
     CDS             174..1115
                     /locus_tag="VKU83_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLI94256.1"
                     /translation="MRRLTFIILVAAIIPGPACNSGDKGFDAEGVFEADEVIVSSEVP
                     GRILQLNLDEGSVLKKDSLVAVIDSVPLVLQRSQVEATIGALGQRTMDVRPQVKMLEA
                     QMAAQQAQLSDLEMEKGRTERLIRSEAAPGKQLDDYNAQIAVLGKQIEATREQINVQR
                     ATVGTQNSTVWSEYRPLRRSVAQIQDQVNRTCVFNPAAGTVLTKYAMAGEVTAAGKAL
                     YKIADLSVITLRIYVSGTQLAGLRIGQRVRVFIDKGPKNYREYPGLITMISDKAEFTP
                     KTIQTKEERANLVYAVKVHVKNDGLLKIGMYGQVKFN"
     gene            1118..2041
                     /locus_tag="VKU83_11625"
     CDS             1118..2041
                     /locus_tag="VKU83_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI94257.1"
                     /translation="MDNAVQIEGIIKRYRSGRSTVTALDKVSFDVRNGEIFGLIGPDG
                     AGKTSLFRVLTTLLLPDGGHAAVNGFDIVREYKRIRRCVGYMPGRFSLYQDLSVEENL
                     GFFASIFKADVQANYDLIGDIYDQIKPFARRRAGQLSGGMKQKLALCCALVHRHAVLF
                     LDEPTTGVDAVSRKEFWEMLHRLKQQGIAILVSTSYMDEAERCDRVALIQKGAILSID
                     SPAGIRGRFGKPLWAVRSAEMYKLLCELQQDPAVESCYPFGQYHHVVLRDAEPEARLN
                     AILRKGGYTDTELRPIAPGIEDCFMDLMKTG"
     gene            2043..>2226
                     /locus_tag="VKU83_11630"
     CDS             2043..>2226
                     /locus_tag="VKU83_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI94258.1"
                     /translation="MDQPAVISVSHLTKRFGTFTAVDDISFDVHKGEIFGFLGANGAG
                     KTTAMRMLCGLSKPTAG"
BASE COUNT          495 a          614 c          682 g          435 t
ORIGIN      
        1 catgcagcct ttgcgcaatt ggcggaaggc gtcatcagtg cgcacgacta cctcgtgcag
       61 gttgacgcgg aagaccaggc gcggcaggct tatattcttc atcaggtcca atgctggcag
      121 gcggagtaca attttcagga ccataccgga aatcatccat aaatgtctat tgtatgcgaa
      181 gacttacttt tattatccta gttgccgcta tcatccccgg gccggcctgc aattccggag
      241 acaagggatt cgatgccgag ggggttttcg aagcggacga ggtcatcgta tcctcggagg
      301 tgcccggaag gattttgcaa ctgaacctcg acgaaggctc ggtgttgaag aaagacagcc
      361 tcgtggccgt catcgattcg gtgcccctgg tgctgcaacg ctcgcaggtc gaggcgacga
      421 tcggcgcgct gggtcagcgg acaatggacg ttcggccgca ggtgaaaatg ctggaagcgc
      481 agatggccgc acaacaggcg cagctgtcgg acctggaaat ggagaagggc cggacagagc
      541 ggctgatccg gtccgaagct gctcccggca aacaactgga tgactataac gcccagattg
      601 ccgtgctcgg caaacagatc gaggcgacga gggagcagat caatgtgcaa cgggctacgg
      661 tcggaacgca gaacagcacg gtgtggagcg aataccggcc gctgcgccgg tccgtggcgc
      721 agatccagga tcaggtcaac aggacctgtg tattcaaccc cgccgccggg acggtgctga
      781 cgaaatacgc gatggccgga gaagtgaccg cggccggcaa ggcgctctat aagatcgcgg
      841 acctctccgt gatcactctg aggatctacg ttagcgggac ccaactagca gggctgagga
      901 tcggtcagcg ggtccgcgtt tttatagaca aggggcccaa aaattaccgc gaatatcccg
      961 gactgataac gatgatctcg gacaaggcgg agttcacgcc gaagaccatc cagacaaagg
     1021 aggagcgggc caacctggtc tatgccgtga aggtgcacgt gaaaaacgac ggcttgttga
     1081 agatcggcat gtatgggcag gttaaattca attagcgatg gacaatgcgg tacagattga
     1141 aggcatcatc aaacgttatc gaagcggccg gtcgaccgtc acggcgttgg acaaggtctc
     1201 attcgacgtg cggaatgggg agatcttcgg gttaatcgga cccgacggcg ccgggaaaac
     1261 atctctcttc cgggtactga ccacgctgtt attgcccgac ggcggccatg cggcggtgaa
     1321 cggtttcgat atcgtccggg agtataagcg catccggcgc tgcgtcggtt atatgcctgg
     1381 gcggttttcg ctgtaccagg atctttccgt ggaagagaac ctcggcttct ttgcctccat
     1441 cttcaaggct gacgtgcagg cgaactacga cctgatcggg gacatctacg accagatcaa
     1501 gccctttgcc aggcggaggg ccgggcagct ctccggcggg atgaagcaga aactcgccct
     1561 atgctgcgct ctcgttcacc gccacgcggt gctttttctc gacgagccga cgaccggcgt
     1621 ggatgccgtt tcccgcaagg aattttggga aatgttgcac cggctcaagc aacaaggcat
     1681 tgccattctc gtatccacct cttatatgga tgaggcggaa cgctgcgacc gtgtcgccct
     1741 gatccaaaag ggcgccatcc tgtcgatcga cagccccgcg ggcatccgcg gccggttcgg
     1801 caagcccctc tgggcagtcc gctcggctga gatgtacaag ctgctttgcg agctgcagca
     1861 ggaccccgcg gtggaaagct gttatccctt cggtcaatat caccatgtcg tattgcgcga
     1921 cgcggagccg gaagcacggc tgaacgccat cctcaggaaa ggcggttata cggatacgga
     1981 actgagaccg atcgccccgg gaatcgagga ttgtttcatg gacctcatga aaacaggata
     2041 atatggacca gcccgccgtc atatcggtat cccatcttac caaacggttc ggaaccttta
     2101 ccgccgtcga cgatatttcc tttgacgtgc ataaaggcga gattttcggc tttctgggag
     2161 ccaacggcgc cggcaagacg accgccatgc ggatgctttg cggcctttcc aagccgacgg
     2221 ccggcg
//
LOCUS       DATGKE010000686         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1084029, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000686 DATGKE010000000
VERSION     DATGKE010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084029"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VKU83_11635"
     CDS             complement(<1..1517)
                     /locus_tag="VKU83_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94259.1"
                     /translation="MFKNYFITSWRNLLRDKSYSSFNILGLAVGMGVALLIGLWVQYQ
                     FSYDRWLPQYRQAFRMMVRSTHNGESDAGMATSLPLAGVLKQNIPEIRYVAQADWIAP
                     HSLVSGDKKLYSSGIFAGDEFLQIFPYPLLQGNAANILQEPASIVITRATATALFGSA
                     DPINKTIKLDNTHDLKVTGILADLPGNSSLQFNYIIPFSFYIQTQDWIRHNWDNWNMD
                     PIQTFVALQPGATQAQIGPRLEAVIKKYNPDGYRAYRLEPFLHPLSDWHLYSNFDNGR
                     QAGGFIDYVRMFSLIGLVVLVIACINFVNLSTARSERRAREVGIRKAIGSRRTDIMLQ
                     FLVEALVISSIAFALALLFVSWALPGFNALTGSEIAIPWTNAFFWGAMIAYVVVTSLL
                     AGSRPALYLSAMRPIKVLKNTVREGKAAALPRKILVVLQFTCSVALIISTVIVYRQIQ
                     YTRNRPKGYDTDRLVMTPGSGDLDHDYAALKSDLLRTGVVTSVTKATAPATALYS"
     gene            1655..>2361
                     /locus_tag="VKU83_11640"
     CDS             1655..>2361
                     /locus_tag="VKU83_11640"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HLI94260.1"
                     /translation="MKQHILSLSQARKIILHAAGLARPAQFGKGREAVYKVIEHLGFV
                     QLDTNYTVERAHHHAIGARVPDYKLEWLEELQADGRIFEFFTSDSGYMPMHDFRFSLP
                     VKEAFAANRKPLTAAEINLMNRVLDRIAREGPLMVKDFENDRVEASAGWWDWRPAKIA
                     LERLYLDGRLMTTRTDKFQKIYDLPMNLVPDDIDKTMPSPEEFARHVIRLGLRCLGIA
                     YVKELAWRARFVKGHMVR"
BASE COUNT          528 a          575 c          718 g          540 t
ORIGIN      
        1 cacgagtaga gagcggttgc gggggcggtt gccttcgtaa cgctggttac cacaccggtt
       61 ctcagcagat cgcttttcag tgcggcatag tcgtggtcca ggtcgccgct gccgggcgtc
      121 atgaccagcc ggtcggtgtc atagcccttg ggccggttcc tggtgtattg aatttgccgg
      181 taaacgatca cggtgctgat gatgagggct acggagcagg tgaactgcag gactacgagg
      241 atctttctgg gaagtgcagc cgctttacct tcgcggacgg tattcttgag tactttaatg
      301 ggtcgcatag cggacaggta gagggcaggt ctgctacctg ccaataagct ggtaacgacc
      361 acataggcga tcatggcgcc ccagaaaaag gcgttggtcc aggggatagc gatctcgctg
      421 ccggtaagcg cattgaatcc gggcaaggcc caggatacga acagcaaggc gagggcgaag
      481 gcgatggatg agatcaccag ggcttccacc aggaattgca gcatgatgtc cgtccgccgg
      541 gaaccgatgg cctttcggat gcccacttcc ctggcgcgtc gttccgatcg cgccgtagac
      601 aggttgacaa aattgataca cgcgatcacc aatactacga gaccgatcag gctgaacatc
      661 cgtacatagt cgataaagcc gccggcttgt ctgccgttgt cgaaattcga atacagatgc
      721 cagtcgctca gggggtgaag gaagggctcg agccgatagg ccctataacc atccgggttg
      781 tattttttta ttacggcctc gagcctgggg ccaatttgcg cttgggttgc gcctggctgc
      841 agtgcgacaa atgtttgaat aggatccatg ttccagttat cccaattatg ccgtatccag
      901 tcctgcgttt gtatatagaa gctgaaggga ataatataat tgaattgcag ggacgagttg
      961 ccgggcagat cggccaggat accggttact ttgaggtcgt gtgtgttatc gagcttaatc
     1021 gttttgttta tcggatccgc ggagccgaac agggcggtgg ctgtagcccg ggtgatcacg
     1081 atagaggcgg gctcctgcaa tatattggcg gcgttgcctt gcagcaaggg atagggaaat
     1141 atttggagaa actcatcgcc ggcaaatatg ccgctgctat aaagcttttt gtctcccgaa
     1201 accaggctat gcggtgctat ccaatcggcc tgggcgacat agcggatctc cgggatattc
     1261 tgtttcaata cacctgccaa gggcaaggaa gtagccatgc cggcgtcgct ttcgccgtta
     1321 tgggtggacc ggaccatcat ccggaaggcc tgccggtatt gcggcaacca acggtcgtaa
     1381 gaaaactggt attgcaccca caggccgatc agcaaggcaa cgcccatgcc gacggcgaga
     1441 ccgagaatgt tgaagctgct atagctttta tcccggagaa ggtttcgcca ggaggtgatg
     1501 aagtagttct taaacatggc agcgaatttt cttccaaatt gatgccatgt cttaattcat
     1561 ttgatattca gggcattgtc gaattttcct tgccgggacc gttcggttat gatacatcgg
     1621 ttgtccggga aaagaaaatg agtaagttgc agctatgaag caacatatcc tgagcctgtc
     1681 gcaggccaga aagatcatcc tgcatgcggc ggggttagcc cggccggcgc aattcgggaa
     1741 gggtcgcgag gcggtctata aggtgatcga gcatctcggt tttgttcagc tcgataccaa
     1801 ttatacggtg gagcgggcgc accatcatgc cattggcgca cgtgtgcccg attacaaatt
     1861 ggagtggctt gaagagctgc aggcggatgg ccggatcttt gaattcttca cctccgactc
     1921 ggggtatatg ccgatgcacg attttcgttt ctccctgccc gtgaaagaag cttttgcagc
     1981 caatcgaaag ccgctcacgg ctgccgagat caatctgatg aacagggtac tggacaggat
     2041 tgcccgcgaa ggcccgctga tggtcaaaga tttcgaaaac gaccgggtag aggccagcgc
     2101 cggctggtgg gactggcggc cggcaaaaat agcgctggaa cggctctatc tggatggcag
     2161 attgatgacc acccggacgg acaagttcca gaagatctat gatctgccga tgaacctggt
     2221 tccggatgac atcgacaaga cgatgccttc gccggaggag ttcgcccggc atgtcatccg
     2281 gcttggattg cgctgtctgg gtatcgctta tgtgaaggag ctggcctggc gggcgcgttt
     2341 cgtgaagggg catatggttc g
//
LOCUS       DATGKE010000687         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_528985, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000687 DATGKE010000000
VERSION     DATGKE010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528985"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="purS"
                     /locus_tag="VKU83_11645"
     CDS             complement(<1..213)
                     /gene="purS"
                     /locus_tag="VKU83_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HLI94261.1"
                     /translation="MNFSVQVKVMPLKELLDPQGKAVMGGLQSLGLRNVRDVRIGKSI
                     HLQIEADNADEARKVAEEAGKKLLANP"
     gene            complement(210..974)
                     /locus_tag="VKU83_11650"
     CDS             complement(210..974)
                     /locus_tag="VKU83_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLI94262.1"
                     /translation="MKLIPNLFTLLNLFFGCLAVILILQNGITTLDNADGTQFIAMPT
                     RTALASIFIGLAAVVDFLDGLVARLFKATSPFGKELDSLADVVSFGVAPGMIIYQLLR
                     LSYASQADALDVSVGWLLPALLIPCAAAYRLARFNIDDTQQYGFKGVPVPAAGLLIAS
                     LPLIGWYTSSPAVKSVLLNKWALYAIILVVAWLMVSRLPLMALKFNHSSFRNNMPKLL
                     LVVLAVIAAIFLHWLAVPVVFVIYIIVSLAFKNKKV"
     gene            complement(1000..2277)
                     /locus_tag="VKU83_11655"
     CDS             complement(1000..2277)
                     /locus_tag="VKU83_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HLI94263.1"
                     /translation="MPDLFAGNRSRFAALLEPGSIAIFVSNDEMPSNGDAHYPFRQSS
                     NLFWLSGIVQEDSMVILFPDHPDPRYKEVLVLVRPNEQKEKWDGRRLRAEEATRISGI
                     RTVVWLDVLDGLLQQWVHLADNIYLDSNENDRRSGPLLTRQHRYICEVKERYPLHRYM
                     RSAKILRQLRAVKTAAEVALMQKAAEITGDVFRHLLSFIRPGVMEYEIEAEIWKGFLS
                     RRSTGPAYECIVASGDRARTLHYIANDGECRGGELVLMDFGAAFGGYNADMTRTVPVS
                     GKFTGRQRELYDAVLSLHGDAKRLLRPGITLADYNKKMGERAAEVFIGVGLLTAGEVR
                     DQTPESPAYLKYMYHGVSHHLGIDVHDLGPRTEPLQEGMVLTVEPGVYVAEEGIGIRI
                     ENDIWIQPGGNVDLMANVPITADEIEGWMNKHK"
BASE COUNT          593 a          782 c          635 g          570 t
ORIGIN      
        1 cggattggcc aacagctttt tcccggcctc ctcggccacc ttccgcgcct cgtcggcatt
       61 gtccgcctcg atctgcaggt gaatgctttt cccgatcctg acgtcccgta cgttccggag
      121 ccccaggctc tggagccctc ccatgacagc ctttccctgc gggtccagca attctttcaa
      181 cggcatcacc ttaacctgca ccgagaaatt cataccttct tatttttgaa cgccaaagat
      241 acaataatgt agataacgaa taccactggc actgccaacc agtgaaggaa tatcgcggcg
      301 atcacggcca ggaccaccag caaaagcttc ggcatattat tccggaagga agaatggtta
      361 aacttcaacg ccatcaacgg cagcctgctg accatcagcc aggcaaccac caggataatg
      421 gcatacagcg cccacttatt cagcaaaacg cttttcacgg cggggctgct ggtgtaccag
      481 ccgatcaggg gtaacgaagc aatcagcaac ccggctgccg gcacgggcac gcccttgaat
      541 ccgtattgct gggtatcatc gatattgaaa cgcgccaggc ggtacgcagc cgcgcatggg
      601 ataagtaaag cgggcaacag ccagccaacg gagacgtcca gggcgtcagc ctgcgatgcg
      661 taactgagcc gcaacagctg gtagatgatc attcccggag cgaccccgaa actgaccacg
      721 tccgcaagtg aatccagctc ctttccaaaa ggactcgtcg ccttgaacaa ccgcgccaca
      781 agtccatcca ggaagtcaac caccgcggcg aggccgatga agatcgaggc cagcgcagtt
      841 cgcgtcggca tcgcgatgaa ttgtgtgcca tccgcgttgt ccaacgtcgt aatgccattc
      901 tgcaggatca ggataaccgc gagacagcca aaaaacaaat tcaacaacgt gaacaggttg
      961 ggtattaact tcattgccga aaattaggac attatttact tatttgtgct tattcatcca
     1021 tccttcgatc tcatcggcgg tgatcggcac gtttgccatc agatcgacgt taccgcctgg
     1081 ctggatccag atgtcgtttt cgatccggat cccaatccct tcttcggcta cataaacccc
     1141 cggctcaacg gtgaggacca tgccttcctg caaaggttcc gtccttggcc ccaggtcatg
     1201 cacgtcgatc ccgagatgat gcgacacgcc atgatacatg tatttcaaat acgcggggct
     1261 ctccggcgtc tgatccctga cctcgcccgc cgtgagcaag ccgacaccga taaaaacctc
     1321 tgccgcccgc tcccccattt tcttgttgta atccgcgagg gttatccccg gccgcagcaa
     1381 gcgcttcgca tccccgtgca gcgaaagcac ggcatcgtac agctcccgct gccggcccgt
     1441 gaacttcccg cttaccggca ccgtccgggt catatcggca ttatagcctc cgaatgccgc
     1501 cccgaaatcc atcaggacga gctcaccgcc ccggcactca ccgtcattgg caatgtaatg
     1561 cagcgtcctg gccctgtctc ccgaggcgac gatacattcg tacgccggac cggtcgaacg
     1621 cctggacaga aaacctttcc agatctcggc ctcgatctcg tattccatca ctcccgggcg
     1681 gataaaggac aacaaatgcc ggaaaacatc cccggtgatc tccgcggcct tctgcatcag
     1741 ggccacctct gccgccgtct tgacagcccg cagctggcgc aggatcttcg ccgagcgcat
     1801 gtagcggtgc agggggtacc tttcctttac ttcgcagatg taccggtgct gacgggtgag
     1861 caaagggccg ctgcgcctgt cgttctcgtt cgaatcgagg taaatattat ccgcgaggtg
     1921 gacccactgt tgtaataacc cgtcgaggac gtccagccag acgaccgtcc ggataccgga
     1981 gatccttgtt gcttcttccg cgcgtaatcg ccgcccatcc catttttcct tttgctcgtt
     2041 cggccggacc agcaccagga cctctttgta gcggggatcc ggatggtcag ggaagaggat
     2101 gaccatactg tcttcctgca cgataccgga caaccaaaaa aggttgctgc tctgccggaa
     2161 cggataatgc gcgtccccat tagagggcat ttcatcattg ctgacaaaga tggcgatcga
     2221 accgggctcc agcaaggccg caaacctgga cctgttcccg gcaaacaagt caggcatttc
     2281 cgggtagtat ttcataatgg ggaaatcagc agggaagcaa aaatattgaa tgattattaa
     2341 atgagccttt tattcggacg gcttcaaact aacgttcgtt agccattagt catatttcaa
     2401 agcagaaggg ctctgatttg attttatcta aatttaggcc cgtttacagg cttgctattg
     2461 gattatttaa ttttagcttt gcaccaaata aacgattgcc tcttatgcca gtgccggtca
     2521 ttgccctccc gacggacagc ttacgtcgtc ttggtctgcg tatttccgat aggatccact
//
LOCUS       DATGKE010000688         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_979416, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000688 DATGKE010000000
VERSION     DATGKE010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979416"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..747
                     /locus_tag="VKU83_11660"
     CDS             199..747
                     /locus_tag="VKU83_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HLI94264.1"
                     /translation="MSSFGAIYLDSVIRRLLTYKTLGERTFAQLEEKDFHYSPNAQSN
                     SISVIVRHLHGNMISRWTNFLTEDGEKPGRNRDAEFSPPPLDKKGIISLWEEGWTCLM
                     NALRSLAEEDLTRTVTIRHEPLIVVDAINRQLAHYPHHVGQIVYIGKMIRGESWESLS
                     IPRGGSAEFNRQMEEENTKTGH"
     gene            938..>3049
                     /locus_tag="VKU83_11665"
     CDS             938..>3049
                     /locus_tag="VKU83_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94265.1"
                     /translation="MLVQLPPVQTWLVSKVTSRLSKNLHTTVSIDSVEFSLFNKMYLY
                     NVLVKDRQKDTLLFARTVKVNITDWWLFKDQAQLKYIGLEDATVKVQRRTDSVWNYQF
                     LVDYFSSRPGTPAVKDSSHGIQMDIREVELNRIHLEKRDAWRGEDMDLHLGGLRLLAD
                     TVSFSRRVARIRSLRFDQPDFVLTNYKGLRPTPPDDSSAPANDPLHLRLNPGGWDLTA
                     GDVSIRNGSFRHEVPADTSVNDKFDGDHIYFYAVESDFANLRLNRDTLTAQLLLSTKE
                     RSGLEVKRLNARIRWYPEGMEFARLDLETNRSHIHNFFAMRFRTLDDMDDFTTRIHME
                     GRFTDADIAWEDIRWFAPDMSPWDKHLRITGDLQGTVSDLRGRHVQLTAGRNTLLNGD
                     IHLKGLPDIHKTLIEFKSNDFETNYDDMVALFPSLRDIRQPRIDQLKKLSFKGDFVGT
                     IHDFVTRGTIASNLGAITTNLNMEVPAYGPSVYTGTIATDSFYLGRFLDNDDIGRIAF
                     RGKVNGTGLKSGTLSATLDGIISSLEFNHYTYQDIQVNGAVAKRKFNGELVADDPNLA
                     ARLNGLIDFTGTQPKFDFRAEVRKADLSRLNFVDKKVEFNGKFKCNFTGDDIDNFLGT
                     ATIYDASVYKNGERVSFDSLELESSIIDNNKTITVVSNEFDGAIVGEFSIKDLPASFQ
                     TFLNKYYPSYIKPARSVPTNQN"
BASE COUNT          745 a          876 c          772 g          656 t
ORIGIN      
        1 agcctgaagg tactgggcta cctggtagcc caacggggca gcatcggccg gacggaagtg
       61 atccgggtga gatccgacca caccatagtg gctgtcggtg acaaacgtgg cgacgatacc
      121 gcgatgggct attgataatc ggacctgccg cagtaccttt gtgcggacct cgcaacacgc
      181 atccctcaat tcccgattat gagctcattc ggcgctatat acctggattc agtcatccga
      241 cgactgctga catacaagac cctgggggaa aggaccttcg cgcagctgga agaaaaggac
      301 ttccattaca gccccaatgc gcagtccaat tccatttcgg tcattgtccg gcatcttcac
      361 ggcaatatga tcagccggtg gacaaacttc ctaacggagg acggcgagaa accaggccgt
      421 aaccgcgatg ccgagttcag ccctccgccg ctggataaga agggcatcat ttccctttgg
      481 gaggaaggct ggacctgcct catgaatgcc ctccggtccc tggctgaaga agatctgacg
      541 cgcaccgtca ccatccggca tgaaccacta atcgtcgtcg acgcgatcaa ccgtcagctg
      601 gcccactatc cccaccatgt cggccagatc gtgtacatcg gcaaaatgat ccgcggcgaa
      661 agctgggaaa gcttatcgat tccgaggggc ggatcggcgg aattcaaccg ccagatggag
      721 gaagaaaata ccaaaacggg acattaaccg gccctttgct aaacttttct caatccgggg
      781 cgtcgaaccc cgggacgagc cgcttttggt actgaatttg cacatcgcca gttatcgcca
      841 tacttttata ccctgaaatg gttagcccct gagaaagatt tggaaaatat tccgtaacac
      901 attacttatt cttctattat tactagtaac aggatgggtg cttgtccagt tgccccctgt
      961 ccagacctgg ctagtctcta aagtcacttc ccgcctatcg aaaaacctcc atactacggt
     1021 tagcatcgat agcgtcgagt tctccctctt taacaaaatg tacctgtata atgtcctggt
     1081 aaaggatagg caaaaggaca ccctgctttt cgccaggaca gtaaaagtga atatcaccga
     1141 ctggtggtta ttcaaggatc aggcccagtt gaaatatatc ggcctggaag atgcaacggt
     1201 taaggttcag cgccgcaccg actcggtgtg gaattaccaa ttccttgtcg actatttttc
     1261 cagccggccc ggcacgccgg ccgttaaaga cagcagccat ggcatccaaa tggacattag
     1321 ggaagtggag ctgaaccgga tacatctcga aaaacgtgac gcctggcgcg gagaggacat
     1381 ggacctgcat ctcggcggct tgcgccttct ggcggacacg gtcagttttt cccggcgcgt
     1441 tgcgaggatc cggagccttc gttttgacca gcctgatttt gtactaacca actataaggg
     1501 actccgcccg acgccgcccg acgatagtag tgccccggcg aatgacccgt tgcatctccg
     1561 gctcaatccg ggcggctggg acctcaccgc gggagacgtt agcatccgga acggatcctt
     1621 ccgccatgaa gttccggctg acacgagcgt gaacgacaaa tttgatggcg accatatcta
     1681 tttttacgcc gtcgagagcg acttcgctaa cctccggctt aacagggata ccctcacggc
     1741 gcaactcctc ctgagcacaa aggagcgctc gggcctggaa gtgaagcggc tcaatgcccg
     1801 catcaggtgg taccccgaag gcatggaatt cgcccgcctc gacctggaaa ccaaccgcag
     1861 ccatatccat aatttttttg cgatgcggtt ccggacgctg gatgacatgg atgatttcac
     1921 cacccgtatc cacatggagg ggcgctttac agatgcggat attgcgtggg aagatatccg
     1981 ctggttcgct ccagacatga gcccctggga caagcacctc aggatcaccg gcgacttgca
     2041 gggcacggtc tcggaccttc gtggccgcca tgtccagctc accgcggggc ggaatacctt
     2101 gctcaatggc gatatccacc tgaaaggcct tcccgacatc cataagacgc ttattgaatt
     2161 caaatcgaac gatttcgaaa cgaattatga cgatatggtt gccctcttcc cttccctgag
     2221 ggatatccgc cagccccgca ttgaccagct aaagaaactg agctttaaag gcgatttcgt
     2281 cggcaccatt catgactttg ttacaagggg caccatcgca tccaaccttg gcgccatcac
     2341 gaccaacctg aacatggagg tgcctgctta cgggccgtcg gtttatacgg gaacgatcgc
     2401 caccgacagc ttttacctcg gccgcttcct cgacaatgac gacattggcc gcatcgcctt
     2461 ccgtggcaag gttaacggaa ccggtctcaa gtcaggtacg ctcagcgcca ccctcgacgg
     2521 gatcatcagc agcctggaat tcaatcacta cacgtatcag gatatccagg ttaacggcgc
     2581 cgtagccaag aggaagttca atggtgaact ggttgccgac gatcccaatc ttgccgcgcg
     2641 gctaaacggc ctaatcgact tcacgggaac gcagccgaaa ttcgacttcc gggccgaagt
     2701 gcgaaaggct gacctgagcc ggctgaattt cgtggataaa aaagtggaat tcaacggcaa
     2761 gttcaaatgc aatttcaccg gggatgacat cgacaatttc ctcggcaccg ctactattta
     2821 tgatgcatcc gtctataaga acggtgaacg cgtttccttc gactccctcg agcttgaatc
     2881 cagcattatt gataataata aaaccatcac ggtcgtcagc aacgagttcg acggcgccat
     2941 cgtcggcgag ttttccataa aggaccttcc ggcttccttt cagaccttcc tgaataaata
     3001 ttaccccagc tatatcaaac cggcgaggag cgtccccacc aaccagaat
//
LOCUS       DATGKE010000689         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_616437, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000689 DATGKE010000000
VERSION     DATGKE010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616437"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VKU83_11670"
     CDS             complement(<1..758)
                     /locus_tag="VKU83_11670"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HLI94266.1"
                     /translation="MQTTSHILMIRPVNFNFNAETAVNNAFQSASTAHPQVEALREFD
                     QFVAILRDNGIDVTVIEDSPAPETPDSIFPNNWVSFHEDGTVCLYPMFAPNRRLERNP
                     AVLQALGKRFRVSRTLDLSSYERQGLFLEGTGSMVFDRDNKIAYACLSPRTNKTVLLD
                     FCALTGFTPEIFAAVDVNGQPIYHTNVMMCVADRYVVACLDALPDPDERHHFIRTVGD
                     SGKELIAISLAQMNRFAGNMLQLRNMDGEPLLVMS"
     gene            complement(785..2245)
                     /locus_tag="VKU83_11675"
     CDS             complement(785..2245)
                     /locus_tag="VKU83_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HLI94267.1"
                     /translation="MAKNYRPAAKGAVSVSSEVGRLRRVLVHSPDSGLGKVVPSKAQD
                     WLFEDIVHLDTIRRKEYDYYTKILLYFLDPGKIRGRLGEIDDPANRRAFYKPGHEEFF
                     QSDKVVELQWLLADILTDREIRLKLVASVCAIENCSYDTEKELLGLDPVQLATTFISG
                     SLKDDRMLFAPIPNFIFTRDIGIVINQYVLLNKPAKKARTREALLAKYIFFNHPFFEE
                     YQDKIIELADTHHHFLLPREGDDYKVTLEGGDVMVISKDHLLIGVSERTSLEAAHQAI
                     NVLFERNVVKKITIIRIPKKRDYMHIDTIFTQIKRNVWILLGTFSTKSLKREAADEVQ
                     RILDTTKKEDKLKIIQFRRKDPGNPEYFDNLEDLLTDISRNDLECTEKVKFIYSGNNE
                     FPFDAREQWTDSCNLLALKEGVVLGYDRNDKTVDAFRAGGFEIVQAEDLIRRLEAGEA
                     QPDQLADTLILMPSAELSRARGGFHCMSMPLHRDPL"
BASE COUNT          534 a          616 c          777 g          594 t
ORIGIN      
        1 gacatgacca ggaggggctc gccgtccata ttgcggagtt gaagcatatt tccggcaaac
       61 cggttcatct gagccaggct gatggcaatc aactcctttc ccgagtcgcc gaccgtgcgg
      121 ataaaatgat ggcgttcatc ggggtccggc agcgcgtcca ggcaggcaac gacgtaccgg
      181 tcggcgacac acatcatcac attcgtgtgg taaatgggct ggccgttaac gtcgaccgca
      241 gcaaagatct ccggcgtgaa gccggtcagg gcacaaaagt cgagaagaac ggtcttgtta
      301 gtccgtggag acaggcaggc gtatgcgatc ttgttgtcgc ggtcgaagac catactgccg
      361 gtaccttcca ggaacagccc ctgtcgctca taggagctga ggtcaagcgt cctgctgacg
      421 cgaaacctct tcccaagcgc ttgcaggacg gcgggattcc gttcaaggcg gcggttcggg
      481 gcaaacatcg ggtagagaca gaccgtgccg tcttcgtgga aggagaccca gttattggga
      541 aagatggaat cgggggtttc cggcgccggg ctgtcttcga tgaccgtcac atcgataccg
      601 ttgtcccgga ggatcgccac aaattgatcg aactcgcgaa gcgcttcgac ctggggatgg
      661 gcagttgaag cggactgaaa ggcgttattg accgcggtct cggcgttaaa gttgaaattt
      721 accggccgga tcatgagaat atgtgaagtc gtttgcatgg atgatcgttt gaaatgcctg
      781 gtaatcataa tgggtcccgg tgtagcggca tgctcataca atggaatccg ccacgggcgc
      841 gtgaaagttc ggcggaaggc atgaggatga gggtatcggc gagctggtcc ggctgcgcct
      901 cacctgcctc gaggcgacgg atgaggtcct cggcttggac aatttcgaag ccgccggccc
      961 tgaatgcatc gacggtcttg tcgttgcggt cgtagccgag tacgacgcct tctttcaggg
     1021 ccagcaggtt acacgagtcg gtccattgtt cccgggcgtc aaaggggaat tcgttgttcc
     1081 cggaatagat gaacttcact ttctcggtgc attccaggtc gttacgactg atatcggtca
     1141 ggaggtcttc caggttatcg aaatattcgg gattgccggg atcttttctt ctgaattgga
     1201 tgatcttcag cttgtcttcc ttctttgtcg tgtcgagaat gcgctggacc tcatcggcgg
     1261 cttcccgttt cagggatttc gttgaaaagg ttccaagcag tatccatacg ttgcgtttga
     1321 tttgcgtaaa aatggtgtcg atgtgcatgt agtcccgctt ttttggaatg cggatgatgg
     1381 tgatcttttt tacgacattc ctttcaaaga ggacgttgat ggcctggtgg gctgcttcca
     1441 gggaagtgcg ctcgctgacc ccgatcagca ggtgatcctt gctgataacc atcacgtcgc
     1501 cgccttcgag cgtgaccttg taatcgtcgc cctcccgggg cagcagaaaa tggtggtggg
     1561 tgtcggcgag ctcaatgatc ttgtcctgat attcttcgaa gaacgggtgg ttgaaaaaga
     1621 tgtatttggc aagcagcgct tcccgggtcc gggccttctt ggcgggtttg ttcagcagga
     1681 cgtactggtt gatgacgatg ccgatatcgc gggtaaagat gaaattggga atgggagcga
     1741 acaacatccg gtcatccttc agcgagccgc tgatgaaagt tgttgcgagc tgtaccggat
     1801 ccagcccgag gagttctttt tccgtgtcgt aagaacagtt ttcgatagcg cataccgagg
     1861 ccaccagttt cagccttatt tcgcggtcgg tcaggatatc ggccagcagc cattggagct
     1921 ccacaacctt gtcggactgg aaaaactctt cgtgcccagg cttgtaaaac gcgcgacggt
     1981 tggccgggtc gtcgatttcg ccgaggcgcc cccggatctt gccgggatca aggaagtata
     2041 agagaatctt tgtataatag tcatattctt ttcgccggat ggtatccagg tggacgatat
     2101 cttcaaacag ccagtcctgc gcttttgagg gaacgacttt tccaagtccg ctgtcagggc
     2161 tgtgcaccag cacgcggcga aggcggccta cttcggagga gaccgaaacg gcgcctttgg
     2221 cggccgggcg gtagtttttt gccatcacca aaaataattc ttttgtgtca aatgggtgac
     2281 agcgctgagc gagcgccagg cggcggggga ggagaggggg taaatccggc cggctcgatt
     2341 gtgatccgtg cggaaattcg taggtttgga aaaaaattgg atgttaccga tcaccgacga
     2401 aacgatcgat cttgtcaacg accggatcca gcagatggat gaggccgaga tcgggaagat
     2461 ctgggacgag ttcgcccgtg agcagccggc gcttgtcggc tatgtgctcg gagaaagcca
     2521 g
//
LOCUS       DATGKE010000690         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1531936, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000690 DATGKE010000000
VERSION     DATGKE010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531936"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..736)
                     /locus_tag="VKU83_11680"
     CDS             complement(32..736)
                     /locus_tag="VKU83_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLI94268.1"
                     /translation="MEGKKPKILLCEDDPNLGMVLKNYLELSDFDVTLERDGRLGLAA
                     FQRERFDLCLLDIMMPHMDGFTLAEEIRDVDPDIPLFFLSAKTMKEDIIQGYKLGADD
                     YITKPFDSEVLLLKIKAILKRNDDLNRESDNKEFDLGSYHFNPKLRELSHKGQMQTLS
                     PKENELLKMLAEHMNDLLPREQALKKIWGSDTYFNGRSMDVYIAKLRKYLKDDDHIEI
                     VNIHGNGFRLVVPVNA"
     gene            824..1195
                     /locus_tag="VKU83_11685"
     CDS             824..1195
                     /locus_tag="VKU83_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HLI94269.1"
                     /translation="MHLTSDIKFKKLVVIGDRVLIRPSRPHERTDSGLYLPPGVQERE
                     KVQQGYVIKTGPGYAIPMPVDEEPWKHQDEQVKYIPLQAREGDIAIFLLSGATEVMYE
                     GEKYFIVPQSAILMLEREEDI"
     gene            1259..2665
                     /locus_tag="VKU83_11690"
     CDS             1259..2665
                     /locus_tag="VKU83_11690"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HLI94270.1"
                     /translation="MDANFNPDRQHTLASATSISGTGLHTGIKVDMTLKPANPGFGFQ
                     FQRVDLPGQPVIKADCDLVTDTSRGTTLEENGAKVCTVEHILAALVGMGVDNCLVEVN
                     GPEIPIIDGSSQPFVEIIEEAGVLEQQATKAWYSIDANITHHDEKKRVEMVVMPSMEY
                     KVTTLIDFNSPVLGTQHAGLKTMRDFKTEISPCRTFCFLHELEMLLDHNLVKGGDINN
                     AIVVVDKPVTAEEMERLAKAFKRDKIEIKSEGYLNNLELRFPNEPARHKLLDVIGDLA
                     LIGYPIKGHIIANRPGHSTNVDFAKRIKQYIKKNKHLKNTPVYDPNQPPVFTTPQIEK
                     TLPHRFPFLLVDKIIELTDRTIVGVKNVTFNEPFFVGHFPGNPVMPGVLQVEALAQTG
                     GILCINAMPAGEYDTYFLKIDNCKFKHKVVPGDTMILKMELLNPIRRGICEMRGTVYV
                     GNKIATEADLMAQLVKRS"
     gene            2815..3621
                     /gene="lpxA"
                     /locus_tag="VKU83_11695"
     CDS             2815..3621
                     /gene="lpxA"
                     /locus_tag="VKU83_11695"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLI94271.1"
                     /translation="MIHSFTSIHPDARLGAGVQVDPFTMIHKNVEIGAGSWIGSNVTI
                     MEGARIGKNCKIFPGAVISADPQDLKFDGEETTVEIDDNTTIREFVTIHRGTRDRWKT
                     TVGRNCMILAYSHIAHDCIIGDNCILSNNSQVAGHVVLGDWVILGGMCAIHQFVKVGS
                     HVFIGGGSLVGKDIPPYIKAARLPLSYSGVNSVGLKRRGFTIDKINHILDIYRVIYNK
                     GMNTSQALEFLEEEFPASDERDEIIGFIRESGRGIIKRFSKSSFDEDYAD"
     gene            3602..>4192
                     /locus_tag="VKU83_11700"
     CDS             3602..>4192
                     /locus_tag="VKU83_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLI94272.1"
                     /translation="MKITLIDAGKRFNRDWIFRHLDYEFLPHRYYAITGPNGSGKSTL
                     LQIIAGAIAATEGVVRYSAGSEIAPEHAYRQLSLSAPYLELIEEMTAIEFLRFHQAFK
                     PFLPSHPLAAILGTVGLAAAAEKQIRFFSSGMKQRLKLAQAIFSASSVVLLDEPCTNL
                     DAEGVALYQSLIRESGNGRLVIVSSNDPQEYDFCETV"
BASE COUNT         1036 a         1136 c         1072 g          948 t
ORIGIN      
        1 gggaacaact actgttttga ttcgcaaaaa ctcatgcatt taccggcacc accaggcgga
       61 acccgttccc atggatattg acgatctcga tatggtcgtc atccttgaga tatttgcgca
      121 gcttggcgat atacacgtcc atgctgcggc cattgaagta ggtgtcgctg ccccagatct
      181 ttttcaaggc ttgctcgcgc ggaagcagat cgttcatgtg ttcggccagc attttgagaa
      241 gctcgttctc cttgggagat aaggtttgca tctgcccctt gtggcttagc tcacgcagct
      301 tgggattgaa atgatacgaa ccgagatcaa attccttatt gtccgattcc ctgttgaggt
      361 cgtcgtttcg cttcaggatc gccttgatct tcagtaagag cacttcactg tcgaagggct
      421 tcgtgatata gtcgtccgct ccgagtttgt agccctgaat gatatcctct ttcatcgtct
      481 tcgcgctgag gaagaacaag ggaatatcgg ggtcgacatc gcggatctcc tccgcgagcg
      541 taaaaccatc catatgcggc atcatgatat ccagcaggca aaggtcgaag cgctcgcgct
      601 ggaaggctgc cagcccaagc cggccgtccc gctcaagggt cacatcaaaa tcgctcagtt
      661 ccagataatt cttcagcacc atccccagat tggggtcgtc ttcgcacaac agtattttcg
      721 gtttcttgcc ttccatattt gatgatgttt agtattaata aaattagatc attggttctc
      781 aaaatcgact aatattttgt taaaccgccc taaatttgcc atcatgcacc tgacttcgga
      841 cattaaattt aagaaactag tcgttattgg cgacagggtg ttgataagac cttcccgccc
      901 gcatgaaagg acggactcag ggttgtatct tcctcccggg gtgcaggaaa gggagaaggt
      961 tcagcaggga tatgtcatta agaccgggcc gggatatgcc atcccgatgc cggtggacga
     1021 ggagccgtgg aagcaccagg atgaacaggt taaatatatt cccttgcagg cgagggaagg
     1081 cgacatagcc atattcctgc ttagcggggc cacggaagtg atgtacgagg gcgaaaagta
     1141 ttttatcgtt cctcaaagcg ccatcctgat gctggagcgg gaggaagaca tttgaccggg
     1201 ggttttcctg gccgattccc cttatctttg tcggctattg gagaattaag taaatcgaat
     1261 ggacgcaaat tttaaccctg acagacagca caccttagcc tctgcgacca gtatttcggg
     1321 aaccggactt cataccggca tcaaggttga tatgacgttg aagcccgcca acccgggatt
     1381 cggcttccag ttccagcgcg tcgacctgcc aggacagccg gttattaagg ccgactgcga
     1441 cctggtaacc gatacttccc gcggcacaac cctcgaagaa aacggcgcaa aggtctgtac
     1501 cgtggagcat atccttgctg cactggtagg tatgggcgtc gacaactgcc tcgtggaggt
     1561 aaacggcccg gaaatcccga tcatcgacgg cagttcacag cctttcgtag agattataga
     1621 agaggcgggc gtcctggaac agcaggcgac caaagcctgg tacagcatcg acgccaatat
     1681 cacccaccat gacgagaaga agcgtgtgga gatggtcgtt atgccatcga tggaatacaa
     1741 ggttaccacc ctgatcgatt tcaacagccc cgtcctcggc acccagcatg cggggctgaa
     1801 gacgatgcgc gatttcaaga ccgagatctc cccctgccgg acattctgtt tcctgcacga
     1861 gctcgaaatg ctgctcgacc ataacctggt gaaaggaggc gatatcaaca atgcgattgt
     1921 cgtcgtcgac aaaccggtca ccgccgagga aatggagcgg ctcgccaagg cattcaaacg
     1981 agataagatc gagataaaga gcgaaggcta tctcaacaac ctcgagctcc ggttccctaa
     2041 tgagccggcg cgccataaac tgctcgatgt catcggtgac ctggcgctga tcggctatcc
     2101 catcaagggc cacatcattg ccaaccggcc ggggcattcg acgaatgtcg attttgcaaa
     2161 acggataaag cagtacatca aaaagaacaa gcaccttaag aatacccccg tctacgaccc
     2221 caatcaaccg ccggtcttca ccaccccgca aattgaaaag accctgccgc accgctttcc
     2281 ttttctcctg gtcgacaaga tcatcgagct gaccgacagg accatcgtcg gcgtgaaaaa
     2341 cgtcaccttc aacgaaccat tcttcgtcgg gcacttcccc ggcaaccccg tcatgccggg
     2401 cgtcctgcag gtcgaggcgc tcgctcaaac cggcggaata ctctgcatca acgcgatgcc
     2461 cgccggtgaa tacgacactt atttcctgaa gatcgacaat tgcaaattca aacacaaagt
     2521 cgtgccgggc gataccatga tcctcaagat ggagttgctc aatccgatcc gccgcggcat
     2581 ctgcgagatg cgcggaacag tgtatgtagg caacaagatc gcgaccgagg ccgacctcat
     2641 ggcacaactg gtaaagagaa gttaagtgaa gttaaattgg gggaaatgca gtaaataagt
     2701 ttatttttgt gagattctgg gtatttgtca gcaatattta tgaagaaaac aggcatctcc
     2761 aaggacgagg taataatgat tcaagaaact gaaaaccaac caagtaacgg ccgaatgatc
     2821 cattctttca cctccatcca cccggatgcg cgcctcggcg ccggtgttca agtggaccca
     2881 ttcaccatga ttcataaaaa tgttgagatc ggagcaggat cctggatcgg gagtaatgtg
     2941 acgatcatgg agggtgcgag gatcggaaaa aattgcaaga tctttccggg ggccgttatc
     3001 tctgcagatc ctcaggacct taaattcgat ggcgaagaaa caacggttga gattgatgac
     3061 aatacaacca tccgggaatt tgtgaccatc catcgcggca cgcgtgaccg atggaaaacg
     3121 acggtgggga gaaactgcat gatcctggcc tacagtcata tcgcgcatga ctgtatcatc
     3181 ggggacaatt gcattctcag caacaactca caggtagccg gtcatgtggt tctcggtgat
     3241 tgggttatcc ttggtggaat gtgcgcgatc caccagttcg tgaaggtcgg atcgcacgtt
     3301 ttcatcggtg gcggttctct cgtcggcaaa gacattccgc cttatatcaa ggcggcccgg
     3361 ctgccactga gttattccgg cgtcaattcg gtcggcctga aacgcagggg cttcaccatc
     3421 gacaagatca accatattct cgatatctac cgcgtaatct ataataaggg catgaatacc
     3481 agtcaggccc tcgaattcct cgaagaagaa ttccctgcca gcgacgagcg tgacgagatc
     3541 atcggcttta tccgcgagag cgggcgcggg atcattaaac gcttcagcaa aagcagcttt
     3601 gatgaagatt acgctgattg atgccggcaa gcgcttcaac cgcgattgga tctttcgtca
     3661 cctggattac gaattcctcc cccatcggta ctatgccatc accggtccca acggatccgg
     3721 caagtcaacc ctcctgcaga tcattgcagg cgccatcgcc gccacagaag gcgtcgtccg
     3781 gtattcagcc gggtcggaga tcgcgccgga gcatgcctac cgccaacttt cgctgtccgc
     3841 accctacctc gaactcatcg aagagatgac cgccatagag ttcctccggt tccatcaggc
     3901 tttcaaaccc tttctgcctt cgcatccgct tgcggccatc ctcggcaccg ttggactcgc
     3961 cgccgctgcg gagaagcaga tccgtttctt ctcttccgga atgaagcagc gactgaaact
     4021 cgcccaggcc attttttccg ccagttcggt cgtcctgctg gacgaaccct gtacgaacct
     4081 ggatgccgaa ggtgtcgccc tgtatcagtc gctcattcgc gagtccggca acggacgcct
     4141 ggtgattgtc agctccaacg acccgcagga atatgatttt tgtgaaacgg ta
//
LOCUS       DATGKE010000691         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_47378, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000691 DATGKE010000000
VERSION     DATGKE010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47378"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VKU83_11705"
     CDS             <1..625
                     /locus_tag="VKU83_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94273.1"
                     /translation="GRLWTLVKIAPDQKYSLDYSVLIAIFIHVNHFISLNAPLMTKAH
                     PIPVVIIALVVLLFADFHLSSCTKTNTKTVYDTTVVTVKDTIIVAPTPSKLSLLVGRD
                     WEMDSAYSNYTGPGTGTLVYARGGTSNSQNLDNYSYTWANNGIEWALENGTYYQFPWT
                     IVNGDSTLLKMTVGANLDYARILYVSSTRADIYDSTASYLDVAIPVP"
     gene            785..1495
                     /locus_tag="VKU83_11710"
     CDS             785..1495
                     /locus_tag="VKU83_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HLI94274.1"
                     /translation="MKKGLLLYLPFSGNIADSSGNGNPTHSTGSVLTYDEHGYPDQAF
                     GATGNGERVYVTNNGSIKFDTAWSLSFGFMMADNRTEFLMSMVDPATGQGPSFTLGTT
                     LPGVPGLTVGTEDVSLGCEGYGRNDDINVVDTTALTPVPGCWYNAIAIYYKGSISVYI
                     NGQLISSKAGQGTASNLCAASQIVIGAWWDGDPLSLNGKLDDIRLYNRVLNPAEIARL
                     ACSYQVTSNAQPRTLENR"
     gene            1570..2568
                     /locus_tag="VKU83_11715"
     CDS             1570..2568
                     /locus_tag="VKU83_11715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLI94275.1"
                     /translation="MGLSIIIINYRTPKLLIDCLAELYRDPESQSFEIIIVDNDSRDD
                     SREHITLAFPGVKWHQMAYNAGFSRANNAGMKLATGDTVLLLNADTLPRGEVVAECYR
                     RLKASTFVAAGVQLLNADGSHQISGNYAMRGGLNYLLPLPYLGKAVKWLGERTGAKKP
                     HVSECGVVAEVDWINGAFLMVKKDAITMAGPMDEDFFLYAEEAEWCGRLSKIGKLCLY
                     GDLQVIHFQGSTAVQLYGQAAKGYVNLYDRKGLQLMLSNFVRIRKQFGVGWFLFQLFW
                     YLLDIPVFFGGLVVSRIGGKRMASVGDFGRYCRNVWYIVRKTGIIIRNKPHFYKVL"
     gene            2644..>3295
                     /locus_tag="VKU83_11720"
     CDS             2644..>3295
                     /locus_tag="VKU83_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HLI94276.1"
                     /translation="MALPYFGHSQGYSYTHYDIGDGLAGSTVYSIAQDKDGFIWASTE
                     TGVSRFDGVHFQNFTTKDGLPDVEILRLFGDSRDRVWMAPFGKSICYYYKGKIHNQLN
                     DPVLRDVGVRGLIDHFSEDSTGNILVQEASCLHLISPQGKVTEIDSVNGRSIRLCHGS
                     TESRDGHFLVQIADTVYHFQAGLFKPVMTMNFPDTPGSPNFILLGKDFSVYRSEYRT"
BASE COUNT          790 a          781 c          841 g          883 t
ORIGIN      
        1 agggcggctt tggactctcg tcaaaattgc cccagaccag aaatattcac ttgactattc
       61 cgttttaatt gctatattta tccacgttaa tcattttata tctctaaatg ctccccttat
      121 gacaaaagcg catccgattc cggtagtaat cattgcctta gtagtattgc tctttgctga
      181 ttttcacctt tcatcctgta cgaaaaccaa taccaaaaca gtttatgaca ccaccgtagt
      241 aacggtcaaa gatacgatca tcgtagcgcc gacgccatct aagctatctt tgctggttgg
      301 tcgggactgg gaaatggatt cggcttattc aaattatact gggccgggaa ccggtaccct
      361 ggtttacgca aggggcggta cgtctaactc gcaaaatctg gataattatt cttatacgtg
      421 ggcgaacaac ggcatcgagt gggcgttaga aaatggcact tattatcaat ttccttggac
      481 tatagtgaat ggcgatagca ctttgcttaa aatgaccgtt ggagcgaatt tggattatgc
      541 caggatcctt tatgtcagct ccacgagggc cgatatatat gattcgacgg ccagttacct
      601 tgacgtagcc atccccgtgc cctgatatcc agcccgcagg gtgtcttgta attgtgcttc
      661 aaataaattt accattatgc gtgttctaca tccattatta accaggctat tggttttgtt
      721 atcgtgttgc ttattttttt catgtgtcaa gaatgacaac cagaacccgg acgttgatgt
      781 caatttgaaa aaaggtctgc tgctctatct cccgttctcc ggcaatatcg cggattccag
      841 cggtaacggc aatcccacac attccacggg ttcggtactc acctacgacg agcacggcta
      901 tccggaccag gcattcggtg caacgggcaa cggtgaacgt gtttacgtga ccaacaatgg
      961 ttccatcaaa tttgatacgg catggtccct gtccttcgga tttatgatgg cagataatcg
     1021 gaccgaattt ctcatgagca tggtcgaccc ggcgaccggc cagggtccga gctttacgct
     1081 cggtactacc ctgccgggcg taccaggatt gacagtcggc actgaggatg tatcgctggg
     1141 ttgcgagggg tacggacgta atgacgatat caacgttgtt gatactaccg cgctcacgcc
     1201 agtgccgggc tgttggtaca acgccattgc catttactac aaagggtcga tcagtgtcta
     1261 tatcaacggt cagctgattt cctcgaaggc tggccagggt accgcttcca atctttgcgc
     1321 tgcctcccag atagtcatcg gtgcctggtg ggatggcgat cccctcagcc tgaatggtaa
     1381 gcttgacgat atcaggcttt acaaccgggt gctgaatcca gctgagattg cccggttggc
     1441 ctgctcttac caggtcacat ccaatgcaca gccccgcaca ctggaaaacc gctgatcgcc
     1501 aaaagccgga cctcggggtc gttttccgga taattggtac tttcgcgatc gcaaaatgcc
     1561 agtgacctga tgggccttag cataatcatt atcaactacc ggacaccgaa attactcatt
     1621 gactgtctgg cagaactgta tcgcgacccc gaatcgcaat cgtttgagat tatcatcgtg
     1681 gacaatgatt cccgcgacga tagccgggaa cacataacct tggcattccc gggagttaaa
     1741 tggcatcaga tggcgtataa tgccggattt tcgcgagcca acaacgcagg gatgaaactc
     1801 gcaacgggcg atacggtgtt attgctaaac gcggatacac tcccccgggg agaagttgtc
     1861 gcggaatgtt atcgaaggct caaggcatcc acatttgtgg cggctggcgt tcaactattg
     1921 aacgctgacg gaagtcacca gatctccggc aattatgcca tgcggggcgg ccttaattac
     1981 cttcttcctc ttccttatct cggaaaggcc gtcaaatggc tcggcgagcg gaccggggca
     2041 aagaagcccc atgtgtccga gtgcggagtg gtggccgaag tcgactggat taatggagca
     2101 tttcttatgg taaagaagga cgctattaca atggcgggac cgatggatga agactttttt
     2161 ttatatgctg aggaggcgga atggtgtgga cggctgagca agataggcaa actttgcctg
     2221 tatggggatt tgcaagtaat tcattttcaa ggaagcacgg ctgttcagtt atatggtcag
     2281 gctgcaaagg gatatgtcaa tctttatgat aggaaaggat tgcaattgat gctgtccaat
     2341 tttgtgcgga ttagaaaaca gtttggcgtt ggctggttcc tctttcaact tttctggtat
     2401 ttattggata tcccggtttt tttcgggggc ctggtcgttt cccggatcgg gggaaaaagg
     2461 atggcttccg tgggcgattt cggcaggtat tgccggaatg tctggtacat agtgagaaaa
     2521 actgggataa tcatccgcaa caaaccccat ttttacaagg tattgtgatc atttcgcggc
     2581 acaagaaagc ttatcttcga aagccgtatg aaacctttcc ttttcttaac ttggattatc
     2641 ctattggcgc tgccatattt tgggcattcc caggggtaca gttataccca ttacgatatc
     2701 ggtgacgggc tggcaggttc gacggtatat agtatcgccc aggataaaga tggctttatc
     2761 tgggcttcca cggaaaccgg cgtcagtcga ttcgacggtg tgcatttcca aaattttacg
     2821 acaaaggacg ggttgcctga cgtcgagatc ctccggctct tcggagactc cagggacagg
     2881 gtttggatgg ccccattcgg caaatccatc tgctattatt acaagggtaa gattcacaat
     2941 cagcttaatg accctgttct cagggatgtt ggggtgagag gtttgatcga ccatttttcc
     3001 gaggacagca ccgggaatat cctggtgcag gaggcgtcct gcctccatct gatctcacca
     3061 cagggaaaag taaccgagat cgattcggtg aacggcaggt cgatccgcct ttgccacggt
     3121 tcgacagaaa gccgtgacgg ccattttctc gtccagatcg cggataccgt ttatcatttt
     3181 caagccggtc tgttcaagcc ggtcatgaca atgaattttc cggacactcc cggttcgccg
     3241 aattttattt tgcttggaaa ggatttcagt gtttatcgca gcgaataccg gacga
//
LOCUS       DATGKE010000692         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_255310, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000692 DATGKE010000000
VERSION     DATGKE010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255310"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VKU83_11725"
     CDS             complement(<1..871)
                     /locus_tag="VKU83_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HLI94277.1"
                     /translation="MMSPTLPETMRAMVLVKAGTALELRTRPLPRPAKDQVLIRVIAC
                     GICRTDLHVVDGELDHPKLPLIPGHEIVGRIMALGEAVDAVAVGQLVGVPWLGHTCGT
                     CKYCRSGRENLCDKPLFTGYTLDGGYAEYTVAFSGYCFPLPEEYGGPAGAPLLCAGLI
                     GYRAYSMIDPGTKRLGLYGFGAAAHILIQLARYEGKMVYAFTRDGDLPGQDFARQMGA
                     HWAGDSSSMPPEPLDASILFAPVGELVPLALRATDKGGSVICGGIHMSNIPAFPYRLL
                     WEERIVRSVANLTR"
     gene            complement(911..2347)
                     /locus_tag="VKU83_11730"
     CDS             complement(911..2347)
                     /locus_tag="VKU83_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94278.1"
                     /translation="MSVTHNKDLQTKQKALAINLDPKIYGSFTETGAGQDVAAYFFKS
                     GASSGTIAKTMSAYDMTFSDAIYGTQPGRRYVSEARLVAMLEKEYGLLIERLAEKRGD
                     QSTFFAFADTVSALNYHRTNEGHGWMGVRFQLEPNGRFNDVIIHIKLLDNDGVLQQNA
                     VGVLGVNLLYACFYYYTHPNIFLLSLMDNLARDAIQIDMIRFEGPDFAKVDNRLMSLQ
                     LVKYGFSDAALFGPDGKNQQPSEVLHKKHIVVVRGRFRPVINVHMDMLQQGVRQFLQE
                     PDVDHEQVVVFTELTLQALKERDADQTADIDEKDFLDRVDILCAMGQTVLISDFHEYY
                     KLVAYLSKITKLKMGVVLGYPNLEYIFSEEHYRDLPGGILESFATLFSRNVKLFIYPT
                     LRNGIIYNCLRFNPPPHLVDLYRYLIANDKIEDIHHYKESNLQIQTDKVLQMIRENED
                     GWEDFVPAEVAKMIKERCLFGVICEPKA"
     gene            2693..3046
                     /locus_tag="VKU83_11735"
     CDS             2693..3046
                     /locus_tag="VKU83_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HLI94279.1"
                     /translation="MAVRIKRVYEPAAETDGYRILIDRLWPRGMAREKAHVDKWLRDV
                     APSTELRKWFNHDPEKWKEFCTRYKKELKGSAAFAELLKEIKERRSVTLLFGARDEEH
                     NDAVALLRFIAKKGR"
     gene            complement(3069..>3335)
                     /locus_tag="VKU83_11740"
     CDS             complement(3069..>3335)
                     /locus_tag="VKU83_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94280.1"
                     /translation="ERINRSGVKLTHIEDIRTLPAGTFIRSNDQPWLYSEGRLHLWTP
                     FGYEAAISPPQSGAVEVLTPRSVVAAFRAGYKPQQAMAVQTQLR"
BASE COUNT          744 a          864 c          959 g          768 t
ORIGIN      
        1 tcctggtaag attggcaaca gaacggacta ttctttcttc ccacaataag cgataaggga
       61 atgcgggaat attggacatg tggatgccgc cgcagatgac ggacccgcct ttgtctgttg
      121 cccgcaaggc aagggggacg agttcaccta cgggggcgaa aaggatggat gcatcgagcg
      181 gttcaggcgg catcgacgaa gaatcgccgg cccagtgcgc tcccatctgg cgcgcaaaat
      241 cctggccggg cagatcgcca tccctcgtga atgcgtacac cattttgccc tcgtaacgcg
      301 cgagttgaat gaggatatgg gcagccgcgc caaaaccgta taaccccagc cgtttggttc
      361 ccggatcgat catgctgtag gcgcgataac cgatcaaccc ggcacacagc aggggtgcac
      421 ctgcagggcc tccatattct tccgggagtg gaaagcaata accggaaaag gcgacagtgt
      481 attcggcata accgccgtca agcgtatagc ccgtaaaaag cggcttatca caaaggtttt
      541 cccggccgct gcggcaatat ttgcaggttc cgcaggtatg gcccagccat gggacgccga
      601 ccaactggcc aacggccact gcatccactg cctcgcctag cgccatgatg cggccgacga
      661 tctcgtgccc cggaataagc ggcaatttcg ggtggtcgag ttcaccatcg acgacgtgga
      721 ggtcggtgcg gcagataccg catgcgatga cccggatcag gacctggtcc tttgccggcc
      781 gcggcagggg tcgcgtgcgg agttccaggg cggtcccggc cttaaccagt accatcgcgc
      841 gcatcgtctc cggtagtgtg gggctcatca taaatttcgg ccatttcggc tttatcagac
      901 cttttgactt tcatgccttc ggctcgcaga taacgccgaa caaacatctt tcctttatca
      961 tctttgcgac ttccgccggg acgaagtcct cccatccgtc ttcgttttcc ctgatcatct
     1021 gcaacacttt atccgtttgg atctggaggt tgctttcctt ataatggtgg atgtcttcga
     1081 tcttatcgtt ggcgatcaga tagcggtaaa ggtctacgag gtgcggaggc ggattgaagc
     1141 gaaggcaatt atagataatg ccgttgcgca aggtaggata tatgaaaagt ttgacgttgc
     1201 ggctgaaaag ggtggcgaaa gattcgagga tgcctcccgg caggtcgcgg taatgttcct
     1261 cggaaaaaat gtattcgaga ttcggatagc cgagaacgac gcccatcttg agcttggtga
     1321 tcttcgagag gtaagccacc agcttgtaat attcgtgaaa atcggagatc agcacggttt
     1381 ggcccattgc gcaaaggata tcgaccctgt cgaggaaatc tttttcatct atgtcggctg
     1441 tctggtcggc gtcccgctcc ttgagtgcct gaagcgtcag ttccgtaaac acgacgacct
     1501 gttcatggtc gacatccggc tcctgcagga actggcggac gccttgttgc agcatatcca
     1561 tgtggacatt gataaccggc cggaaccgcc cccgcaccac gacgatatgt ttcttatgca
     1621 ggacctcaga tggctgctgg ttcttgccgt ccggaccgaa cagggccgca tcagaaaatc
     1681 catacttgac cagttgaagg ctcatcaacc ggttgtccac ctttgcaaaa tcaggccctt
     1741 caaaccggat catatcgatc tgaatggcat ccctggcgag attatccatc aaagaaagga
     1801 ggaagatgtt gggatgcgta taatagtaga aacaggcata aagcaggttt accccgagga
     1861 cgccaacggc attctgctgg agcacgccat cattatccag cagcttgata tggatgataa
     1921 catcgttgaa cctgccgtta ggttccagtt gaaaacgaac gcccatccat ccatggcctt
     1981 cgtttgtcct gtgatagttg agcgccgaaa cggtatcggc aaaggcaaag aaggtagact
     2041 gatcgccgcg tttctccgcc agtcgttcga tgagcaggcc gtattccttt tccagcatgg
     2101 ccaccagcct ggcctcactc acgtaccggc ggcccggctg cgtcccatag atcgcatcgg
     2161 aaaacgtcat gtcgtaggcg gacatcgttt tggcgatggt tcccgatgag gcgccggact
     2221 tgaaaaaata agcggcgacg tcctgccctg cgccggtttc cgtaaaggag ccgtaaatct
     2281 tgggatccag gttgatggca agcgcttttt gttttgtttg caggtcttta ttgtgggtaa
     2341 cgctcatatt cgccggtgat ttgtcgctgc caataattgg taaagacgcg caaatttacg
     2401 atttattgcg aagatggcgg cttatccggc ggtgttggcc ttgcctgctc ctataccccg
     2461 gtttcgggcc gatgatattt tcgtcgttgt cctgcggggc ggccgtgaag gtcgcggcgg
     2521 tcttaaggaa atcgcggcgg tttcgcggtg ggtgattgct attcatagtt gtagtttaga
     2581 tttcgtccat aaatcagttt gtccgtggtt tcacgggtag caatctttgc tcctgagtgt
     2641 aaggaatttt tagggcataa atcttgcaac tttgcctcat aaaagcgcta ggatggctgt
     2701 ccggatcaaa agggtatatg aacctgccgc agagacagac ggctaccgga tcctgatcga
     2761 ccggttatgg ccacgtggca tggccaggga gaaggcgcat gtcgataaat ggctcaggga
     2821 cgtcgcgccc tctaccgaac tgagaaaatg gttcaaccat gacccggaga aatggaagga
     2881 gttttgcacg agatacaaga aggaactgaa aggatccgcg gcgttcgcgg agctgctaaa
     2941 ggagatcaag gagcgccggt ccgtcacctt attgttcggc gccagggacg aggagcacaa
     3001 tgatgccgtg gcgttgctgc gtttcatcgc aaaaaagggg cgataggctg tgctgccgga
     3061 gaacaccgtc accgaagctg ggtctggacc gccattgctt gctgcggctt atagcccgcc
     3121 cggaatgcag cgaccacaga tcttggtgtc agcacttcga cagcgccgga ctgcggcggc
     3181 gaaatggctg cttcgtaacc gaaaggcgtc cacaggtgga gacgtccctc cgaatagagc
     3241 cagggttggt cattcgaccg gataaaggtt ccggcgggaa gtgtcctgat gtcttcaatg
     3301 tgtgtgagtt tgacgccgct gcggttgatc cgctc
//
LOCUS       DATGKE010000693         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1044225, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000693 DATGKE010000000
VERSION     DATGKE010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044225"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..717)
                     /locus_tag="VKU83_11745"
     CDS             complement(139..717)
                     /locus_tag="VKU83_11745"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLI94281.1"
                     /translation="MNPKVDAFLSRAAKWQKEMEKLRAIALDCGLNEELKWGKPCYTF
                     QGKNVVVIQGFKDYCALLFFKGYLLSNAGGLLVKTGENTVVGRQARFRSVAEITGAKA
                     DLKASIYEAIEVEKAGIKPAAAEKAAIKLPEELQTRLNKNPTLKKAFHALSPGRQRGH
                     IFYISQAKQSKTRESRVDKSAKQILAGKGPND"
     gene            complement(728..1111)
                     /locus_tag="VKU83_11750"
     CDS             complement(728..1111)
                     /locus_tag="VKU83_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLI94282.1"
                     /translation="MTKTTRIIYWVATIWLALGMLAGGLQQIFKVKAFVDIMVHLNYP
                     LYVLYILGVWKVLGAVAILLPGLNLLKEWAYAGFFFAMSGAVFSHLAVGDTMKEIYPS
                     LSLLLLTVASWYFRPVDRRPVLAHS"
     gene            complement(1118..1585)
                     /locus_tag="VKU83_11755"
     CDS             complement(1118..1585)
                     /locus_tag="VKU83_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLI94283.1"
                     /translation="MEPKTKVHAEAGKQEYLITREFDLPVHLLFKAHVDPDIVAQVMG
                     TKVLSFEAKKNGSYRFETTDPQGKVIFQAAGVIHELIPDKKIIRTFEMGNTPFGVQLE
                     IYDFEKLSDETSKLTMHVIYESVAQRDQVLQIGLTWGINMAHDRIQEIAGKLK"
     gene            complement(1604..1918)
                     /locus_tag="VKU83_11760"
     CDS             complement(1604..1918)
                     /locus_tag="VKU83_11760"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLI94284.1"
                     /translation="MNLRRDVFQAIADPTRRAILLLVATQTMTAGAIAANFDTARPTI
                     SRHLQILTECELLEPRPNGREIYYQFNPKKMKEVAEFIEPFRKMWDDRFNKLESIMKK
                     YK"
     gene            2126..3142
                     /locus_tag="VKU83_11765"
     CDS             2126..3142
                     /locus_tag="VKU83_11765"
                     /inference="COORDINATES: protein motif:HMM:NF040069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HLI94285.1"
                     /translation="MKIPSTLLAIVVVCYSCSSTNLMSLSVTEPAPVSLPPAAKTAAV
                     VNRTHVADDSRTVDAIHRTISLETKELQAEGAGASVTGLTDALIQSNRFDSVKRLIHL
                     DLRSYGAGVFPVYLSWDTVEKICSMYHVDVLFSLELFDAESRVGVTGVNLAALSAQAN
                     VNTRVRTGWRIYDPATRSVLDQYIIYRDLNFQGGTLIGTGSALLGRKEAVIKAGNRAG
                     QDYASRIIPYSLRVSRFYYVRGNGGFIAARRMAQAGDWNEAAKVWQQATTSPSRKVAG
                     RGCYNMAIISEINGDLNGALQWAKKAYEVYRTPYSLDYVNILQQRLSEDAVLRSQSEL
                     TTSR"
     gene            3305..3793
                     /locus_tag="VKU83_11770"
     CDS             3305..3793
                     /locus_tag="VKU83_11770"
                     /inference="COORDINATES: protein motif:HMM:NF022838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HLI94286.1"
                     /translation="MKKMKLVLMGLAVCAMPFLAEANSGGGKNMPRPVSSAFTRQYPG
                     AHLMNWQERNNEYVVNFRSSTENSSAYYTKDGVWLRTDTKLSGKQDLPENVQDGLARS
                     TYAKYNVEEVDQVRQPAGKTVYMLRVDYGADAGNDYLLYFNADGRLENARRAPGQPSE
                     EQ"
     gene            complement(3797..>4522)
                     /locus_tag="VKU83_11775"
     CDS             complement(3797..>4522)
                     /locus_tag="VKU83_11775"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HLI94287.1"
                     /translation="LSKPETIQAAPPIDSVSIGYDSAGSTYISPPPLIVTVNTHDPGN
                     GQRYYRWSTSGYLPRKSWGSPCDPFSDPPCTNPYMCTCAALCEQKNTASQGLKVISNQ
                     LSEGKEIIEPVYYSPVYWFGNHYVEIDQFSLTLESYQFWEQYLAQSDRTGSILDPLPS
                     PVIGNIYNAADTNKLALGIFSASDVFRKKLVLVPFFLQQYQLEALAGQYILQGDCHFT
                     YPNSLPDDMPPAGWDSAQVINIR"
BASE COUNT          986 a         1222 c         1232 g         1082 t
ORIGIN      
        1 ttcatgccct tttcgcacaa ttccttggca gcccagccgc cgctgatgcc ggagccgatg
       61 acgatggcgt catatgtatt gactgatccc atagtacctt attcagcgcc gcgtaaggta
      121 ctataatttt acaagacctt aatcattcgg ccccttgccc gcgaggatct gcttcgcgct
      181 cttgtcgacc ctggactcgc gggttttcga ttgtttggcc tgggagatat aaaatatatg
      241 tccgcgctgc ctgccgggcg acagggcatg aaaagccttt ttcagcgtag ggtttttgtt
      301 cagcctcgtc tgcagctcct ccggcagttt tatcgcggcc ttttcggcgg cagcgggttt
      361 tatccccgcc ttctccacct cgatggcctc atagatgctg gctttcaggt cggctttggc
      421 gccggtgatt tccgcgacgc tgcggaaccg ggcctggcga cccaccaccg tgttctcgcc
      481 ggtctttacg agcaggccgc cggcgttact cagcaggtag cctttgaaga ataagagggc
      541 gcagtaatcc ttgaatccct ggatcaccac aacgtttttc ccctggaaag tgtaacaggg
      601 tttgccccat ttcagttcct cgttcaggcc gcagtcaagg gcgatcgccc gcaatttttc
      661 catttctttc tgccatttgg cggccctgct caggaaggcg tcaactttgg gattcatgtg
      721 ttttgtttta tgaatgagcc aatacgggtc ttctgtccac cggcctgaaa taccaggatg
      781 cgacggtcaa caggagcagc gacagcgagg gataaatttc tttcatggtg tcccccacgg
      841 caagatgcga aaatacggct cccgacatcg caaagaagaa gcccgcatag gcccactcct
      901 taaggaggtt gagtcccggg agcagtatcg ctactgctcc aagtactttc catacgccga
      961 ggatatacag tacatacagc ggataattca gatgaaccat gatgtcgaca aaagctttca
     1021 ctttgaagat ttgctgcagg ccgccggcca gcattcccag ggcaagccag atggtggcga
     1081 cccaatagat gatccgggtt gtctttgtca tatatttcta ttttaatttt ccagcaatct
     1141 cctggatgcg gtcgtgcgcc atattaatgc cccaggtgag gccgatctgc aatacctggt
     1201 cgcgctgcgc gaccgattca taaataacgt gcatggtaag cttgctcgtt tcatcactaa
     1261 gcttttcgaa atcgtagatc tcgagctgga cgccaaacgg cgtattgccc atttcgaatg
     1321 tccggatgat cttcttgtcc gggatcaatt catggatcac cccggccgcc tggaatatca
     1381 ccttaccttg gggatcggtc gtctcaaacc ggtagctgcc gttcttcttg gcttcgaagc
     1441 tcagcacctt cgtgcccatg acctgcgcga caatatcggg atccacatgc gctttaaaga
     1501 gcaggtgaac gggcagatcg aattccctcg tgatcaggta ctcctgtttt ccggcttcgg
     1561 cgtggacctt ggtttttggt tccatctggt ttcgtttgtc tttttattta tattttttca
     1621 tgatgctttc cagcttgttg aacctgtcgt cccacatttt gcggaacggc tcgatgaatt
     1681 cggctacttc tttcattttt tttggattga attggtaata tatttcccgc ccgttgggtc
     1741 gcggctcgag caattcgcac tcggtgagga tctgaaggtg cctggatatg gtcggccttg
     1801 ccgtatcgaa gttggcggcg atggcgcccg ctgtcatggt ttgggtggca accagcagga
     1861 ggatggccct tctcgtggga tcagcgatgg cttggaaaac gtcccgtctc aggttcattg
     1921 tgtagctatt tgactacaaa tatatgcgta gccattcaac tacgcaaatt tttttttagc
     1981 ctctcctgcc ccccggctgc caaacccgga tcccgctgcc cacgcgcctg ccggccctga
     2041 tgaaaatatg aagaaaattg cactaaatcc cttgtcctgg ccgttatggt atggttagtc
     2101 caatctcctg ataaaccatt cacttatgaa aataccctct accctccttg ccatcgtagt
     2161 cgtctgttat tcctgcagtt ctacgaacct gatgtcgctg agcgtgaccg aacccgcgcc
     2221 ggtaagcctg ccgccggctg cgaagaccgc ggccgtggta aaccggaccc atgtggcgga
     2281 cgacagcagg accgtggatg ccatccaccg gacgatctcc ctagaaacaa aggaactgca
     2341 agcggagggt gcgggcgcga gcgtgacagg ccttaccgac gccctgatcc agagcaaccg
     2401 gttcgactcc gtaaagcggc tcatccatct tgatctccgg tcgtatggcg ccggcgtgtt
     2461 ccccgtgtat ttgtcctggg ataccgtcga aaagatttgc agcatgtacc atgtcgacgt
     2521 gcttttctcc ctggagcttt tcgatgccga atcaagggtc ggggtaaccg gcgtaaacct
     2581 cgcggcgctc agcgcgcagg caaacgtgaa tacccgcgtc aggacgggct ggcggattta
     2641 tgatccggcg acgcgaagcg tcctggacca gtatatcatt taccgcgacc tcaacttcca
     2701 gggcggaacc cttatcggta ccggatccgc gcttcttggc cgcaaggaag cggtgatcaa
     2761 ggcgggtaac cgggcgggcc aggactatgc cagccggatc attccttatt cgcttcgggt
     2821 gtcccgtttt tattatgtcc gcggcaacgg cggtttcatc gcagcgagac gcatggcgca
     2881 agccggtgac tggaacgaag cagcaaaagt ctggcagcag gcgacaacga gcccgtcgcg
     2941 gaaagtggcc ggccggggct gttacaatat ggccatcatc agtgagatca atggcgacct
     3001 taacggagcc cttcaatggg cgaagaaagc ctacgaggta tacagaacac cctattcgtt
     3061 ggattacgtc aatattctcc agcaacggtt atccgaagac gctgtgctca gaagtcagtc
     3121 cgagctgacg acttcccgct aacggggcct ttcaaaaaaa cgaatgatcc acgcggataa
     3181 cggttaactc ccaccggaca gcgcttcagg gaacggtaat tgcaacgata cttcggacag
     3241 gactcctgtc ggttgatcgt tccattaaca ctcctctgtc atcgaacaat taaaatgaca
     3301 gtctatgaaa aagatgaagt tagtcctgat gggcctggcc gtctgcgcca tgccgttttt
     3361 agcggaggca aattccggcg gcggcaagaa catgccccgg cccgtatctt ccgcctttac
     3421 ccggcagtat ccgggcgcac acctgatgaa ttggcaagag aggaacaatg aatatgttgt
     3481 caacttccgc agctccacgg aaaattcatc ggcctattat accaaagacg gcgtctggct
     3541 gcgtaccgat acgaaattat cgggaaagca ggatcttcct gagaatgtac aggatggcct
     3601 ggcccgttcc acctacgcca agtacaacgt ggaagaagtc gatcaggtcc ggcagccggc
     3661 cggtaagaca gtttacatgt tgagagtaga ctacggtgca gatgccggta atgattatct
     3721 gttgtacttt aacgccgacg gccggttgga aaatgcaagg cgtgcgcccg gccagccatc
     3781 cgaagagcaa tagcggttac cggatattga tcacctgggc gctgtcccag ccggcaggcg
     3841 gcatatcgtc gggaagactg ttgggataag taaagtgaca gtcgccttgc aggatgtatt
     3901 gccccgcgag cgcttcgagt tggtattgct gcaggaagaa gggcacgagg accagctttt
     3961 tccggaagac atcggaggcc gagaaaatcc cgagggcgag cttattggta tcggcggcgt
     4021 tataaatatt tccaatgacc ggtgacggca ggggatcgag aatgctgcct gtcctgtcgc
     4081 tctgggcgag gtattgctcc cagaactggt aactctccag cgtcagggag aactggtcta
     4141 tctcgacgta atgattgcca aaccaataaa cgggcgaata atacaccggc tcgatgatct
     4201 ccttcccctc cgacaattga ttggaaatga ccttcagacc ctgggaggcc gtattcttct
     4261 gttcgcaaag cgcggcgcaa gtgcacatgt aaggattcgt gcaaggcgga tcggaaaagg
     4321 gatcgcaggg gctgccccac gatttacggg gcaagtaccc ggaggtcgac caacgataat
     4381 agcgctggcc attccccggg tcatgcgtat tcacggtgac gattaacggc ggaggtgaaa
     4441 tgtaggtgga cccggcgctg tcatagccga tcgaaacgct gtcgatgggc ggcgccgcct
     4501 ggatcgtctc gggcttggat ag
//
LOCUS       DATGKE010000694         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1292772, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000694 DATGKE010000000
VERSION     DATGKE010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292772"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="argH"
                     /locus_tag="VKU83_11780"
     CDS             complement(<1..1137)
                     /gene="argH"
                     /locus_tag="VKU83_11780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLI94288.1"
                     /translation="MSVKKLWSKEGTTTSAAVEAFTVGRDKEFDVLLAEYDVLGSLAH
                     TEMLESAGLLSKQDFGLIRTGLQQILAEIRGGRFAIEPGAEDVHSQVEALLTQRIGEA
                     GKKIHSGRSRNDQVAVDIKLYLKAQVLVIKDEAEKLFRQLIALSEEYRSVLLPGYTHL
                     QIAMPSSFGLWFGAYAESLVDDLELLAAAYQVVNKNPLGSGAGYGSSFPLDREMTTRL
                     LNFRTLNFNSVYAQMSRGKTEKAVAVGLASVAATLGRLATDCCLYINQNFGFISFPAD
                     LTTGSSIMPHKKNPDVFELIRAKCNRIQAVPNELALLTGNLPSGYHRDLQLTKEILFP
                     AIGEGLACLRMTGLMLDHVEVRTNILEDEKYKYLFSVEAVNRLVT"
     gene            complement(1134..>2036)
                     /locus_tag="VKU83_11785"
     CDS             complement(1134..>2036)
                     /locus_tag="VKU83_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI94289.1"
                     /translation="DPAKPTILLNSHHDTVKPNKAYTLDPFMPLEKEGKIYGLGSNDA
                     GGCLVSLIAAFLHYWPDPGMEYNLVLAATAEEEISGHNGVELLLPRLGPVEFGIVGEP
                     TDMQLAVAEKGLLVLDCVAHGKAGHAARDEGENAIYAALKDIRWFSSFRAPKVSDLLG
                     PVKMSVTVIDTDNKAHNVVPSQCHFVVDVRVNELYTFEELLALIKAHVGCDISPRSLR
                     MRSSSIPPDHPIIRSGLRLGRTCYGTPTTSDKALMPFPTLKTGPGDSARSHTADEYIF
                     AAEIHEGIEGYIGLLKPVLYNLAL"
BASE COUNT          393 a          609 c          581 g          453 t
ORIGIN      
        1 ggtcaccagc ctgtttaccg cttcaacgct gaagagatat ttatactttt catcctcgag
       61 aatattcgtc cggacctcga catgatccag catcaggccc gtcatccgca ggcaggccag
      121 tccctccccg atcgccggga aaaggatttc tttcgtcagt tgcagatccc ggtgataacc
      181 cgagggcagg ttgcccgtca gcagcgccag ttcgtttggc accgcctgaa tgcggttgca
      241 ttttgcccgg atgagctcga agacatccgg gttcttttta tgaggcatga tgctgcttcc
      301 ggttgtcagg tcggccggga agctgataaa gccaaaattt tggttgatat acaggcaaca
      361 gtccgtagcc agccggccaa gcgtagcggc taccgaggcc aaacccacgg caacggcctt
      421 ttccgtcttg ccgcgggaca tctgggcgta gacggaattg aagtttaagg tccggaaatt
      481 caacagtctc gtggtcatct cccggtcgag cgggaacgag gagccgtacc cggcgccgct
      541 cccgagcggg ttcttgttaa ccacctggta tgccgccgcg aggagctcca ggtcgtctac
      601 caggctttcg gcataagcgc cgaaccaaag tccgaaagag gagggcatcg cgatttgcag
      661 atgggtataa cccgggagca atactgagcg gtattcttcg cttagcgcga tcagctgccg
      721 gaatagtttt tcagcctcgt cctttatcac cagcacctgc gccttcagat agagcttgat
      781 gtcgacggcc acctgatcgt tacggctgcg gccgctgtgg atctttttgc ccgcctcgcc
      841 gatccgttgc gtgagcagcg cttccacctg cgaatgcacg tcttcggccc ctggctcgat
      901 ggcgaagcgg cctccccgga tttcggcgag gatctgctgc aggcctgtgc ggatgaggcc
      961 gaagtcctgt ttgctcaaga gtccggccga ctccagcatt tccgtgtggg cgaggctgcc
     1021 cagcacgtca tattcggcca gcagcacatc gaattcctta tcgcgaccga cggtaaacgc
     1081 ctcgaccgcc gccgaagtgg tcgtaccttc cttgctccaa agttttttca cgctcataat
     1141 gctagattgt ataacacggg tttcagcagc ccgatatatc cctcgatccc ttcgtggatc
     1201 tccgccgcaa aaatgtattc gtcggccgtg tggctcctgg ccgaatcgcc gggaccggtc
     1261 ttcagggtcg ggaagggcat caaggccttg tccgacgtgg ttggggtgcc ataacaggtt
     1321 cgccccaggc gcagccccga cctgatgatc ggatgatccg gcgggatgga tgacgaccgc
     1381 atccggaggc tgcgcggcga tatgtcgcag cccacgtgtg ccttgatcag ggccagcagt
     1441 tcctcgaatg tatacagctc gtttacccgg acgtcgacca cgaaatggca ctgggaaggt
     1501 accacattat gagccttgtt gtcggtatcg atcaccgtta cgctcatctt taccggcccg
     1561 agcaggtcag agactttagg cgcgcggaag ctgctgaacc aacgaatgtc cttcaatgcc
     1621 gcgtaaatgg cgttctcacc ttcgtcccgg gccgcatgcc ctgccttgcc gtgcgccacg
     1681 caatccagta ccagcaaacc cttttcggca accgccagct gcatgtccgt gggctcgccg
     1741 actattccga attcgacggg gcccaggcgc ggcaaaagga gctcgacccc gttatgtccc
     1801 gagatctcct cttcggcggt cgccgccaac accaggttat actccattcc ggggtccggc
     1861 caataatgca ggaaggcggc gatcagcgag accaggcatc cgccggcatc gttgcttccc
     1921 aggccgtata ttttgccttc cttttccagc ggcatgaacg gatcgagggt atacgctttg
     1981 ttgggcttta cggtatcgtg atgggaattg agcaggatcg taggctttgc gggatc
//
LOCUS       DATGKE010000695         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1126881, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000695 DATGKE010000000
VERSION     DATGKE010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126881"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VKU83_11790"
     CDS             <1..358
                     /locus_tag="VKU83_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94290.1"
                     /translation="EMEEPIDLKDIPAYLAFSGIIIYRKSVALESPKFIDLGKVYGVS
                     QLSVNGVLQGVQWYGKRVFKLEKGENRLEVTVTATMGNYLKTLKTNPVAQYWTNEGSK
                     DQPIQSLGMAGPVRIY"
     gene            360..1784
                     /locus_tag="VKU83_11795"
     CDS             360..1784
                     /locus_tag="VKU83_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HLI94291.1"
                     /translation="MMKYLFIFFLPVAARGADYPASLFGIRSDGATLNTRSIQFAVDF
                     IHDRGGGRLIFDVGRYLTGSIHLKSDVGLELKEGAVLLGSLNPFDYDKDQFTALLLAD
                     SQRDISISGKGIIDGQGRAVAANIVGLVHRGIIQDPLRSDRPNEANRPMLIYFRRCSG
                     VTVKGVTLANAASWVETYDQCAGLAIDSIRVDSKAYWNNDGIDIVDCSEVRITGCFVD
                     AADDGICLKSHDPAAACTDVLIRDCTIRSSANAIKLGTVSRGGFRHIRILHNSVYDTY
                     RSALALEAVDGAAIRDLVADSLTAVNTGNALFMRIGARSGSQQSSLADVSIRHLDIRI
                     AAGKADSGYPYEGPVEDQPRNISPVVLAGLPGAFLQDIQLEDVTVRYPGGGKPFTVGR
                     DPARSDSVPELPANYPEFSMFRELPAWGLYIRHARGIRIRNLRLYCAAKDYRKAIVVD
                     DVKGATWEATVVSEPGNTKAFGGN"
     gene            complement(1832..3022)
                     /locus_tag="VKU83_11800"
     CDS             complement(1832..3022)
                     /locus_tag="VKU83_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94292.1"
                     /translation="MRTLFLILSGTIGLKAVGQTDSTTRPEQKDLIDLGRAFFHLRSH
                     PRSATATNVQFSFVPVSTQTAGPAGLTITATTAAFRLVNRSAGNLSTATFAPYVALQG
                     RVGFALRSNLWLPRNSWDILGDIRLLYYPQDTWGLGGSTEYGRQLKINYKYFRVYETV
                     LKRIETSLFAGLGLLIDNRYDLDAPGDSLLLQGFTHYPYGTGYSEQSTSTGISFNLLY
                     DTRTNTQNPLPGSYAHVVYRVNPVWTGSSTPWNSIYVDGRKYVPLSLKRQDILALWVF
                     YWSVLETHAPYFDLPSIGWDPYQQRSGRGFPQNRYRGKALLYGEAEYRHVLTADGLLG
                     FVVFFNANAVSEPATDQLTYFHPAAGAGLRIKFNKKSGTNIALDAGFSKGYTGIYLNL
                     GETF"
     gene            complement(3139..3513)
                     /locus_tag="VKU83_11805"
     CDS             complement(3139..3513)
                     /locus_tag="VKU83_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI94293.1"
                     /translation="MNNAARIIVVDDNNDHLHIIRLILEKFGYEVKTLQRSENVLDTV
                     KAFQPGLIFMDHNVPMFDGIMATKLLKSDAGCKHIPVVYFTSENNIEELAIEAGADDW
                     LRKPFSLEDLIGKITKLLPAGS"
     gene            3779..4150
                     /locus_tag="VKU83_11810"
     CDS             3779..4150
                     /locus_tag="VKU83_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HLI94294.1"
                     /translation="MKQVPGRKPETIDEYIDSYDQDIAAMLRQVRKAIREAAPAATEV
                     ISYGMPAFKQNRVLVYFAANKNHLGFYPTAEPIRVFKNELKDYVTSKGAIHFPLDKAL
                     PVSLIKKIVKFRAAADRAEPN"
     gene            4210..>4402
                     /locus_tag="VKU83_11815"
     CDS             4210..>4402
                     /locus_tag="VKU83_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94295.1"
                     /translation="MKKRTFIKLSSVMMAGTALTPLVDLGQGGELTNWAGNITYGTTR
                     VMRVPSVPEAAELVKRYDRL"
BASE COUNT         1011 a         1088 c         1298 g         1005 t
ORIGIN      
        1 cgaaatggaa gagcctatag atctgaagga tattccggca taccttgcgt tcagcggcat
       61 tattatctac cggaagagcg tcgctctcga atcgcctaaa tttatcgacc ttggcaaagt
      121 ctacggagtg tcgcaactga gcgtgaatgg cgtactccaa ggggtgcaat ggtatgggaa
      181 gcgcgtattc aaactggaga aaggggaaaa caggctggag gtaacggtca cggctaccat
      241 gggcaactat ttgaaaaccc ttaaaaccaa cccggttgcg cagtattgga ccaacgaggg
      301 aagcaaagac cagccaatac aatccctggg tatggcgggg cctgtcagaa tttattgaga
      361 tgatgaaata tctttttatc tttttcttgc cggtggcggc ccggggggca gactaccccg
      421 cttcgctgtt tgggatccga tctgacgggg cgaccttaaa tacccggtcg atacagtttg
      481 ccgtcgattt tatccatgac cgcggaggcg gccggttgat ttttgacgtg ggcaggtacc
      541 tgacgggatc tattcatctt aaatcggatg ttggtctgga attgaaggaa ggggccgtgt
      601 tgctcggctc gctcaatccc tttgattacg ataaggatca gtttaccgcg ttgctgctgg
      661 cggacagtca gcgggacatt tccattagcg gcaagggcat catcgacggg cagggtaggg
      721 cggttgctgc aaatatcgtg ggactggtcc accgtggcat catccaggac ccgctgcgga
      781 gtgaccggcc gaatgaggca aaccggccga tgctgatcta tttcaggcgt tgcagcggcg
      841 tgacggtcaa gggcgtcacg ctggcaaatg cggcttcctg ggtggaaacc tatgaccagt
      901 gcgccggcct ggcgatcgac agcattcgcg tggacagcaa ggcgtattgg aacaatgacg
      961 ggatcgatat cgtggattgc agcgaggtca ggataaccgg gtgttttgtc gacgcggccg
     1021 acgacggcat ttgccttaaa tctcatgacc cggccgcagc ctgcacggac gtgctgatcc
     1081 gcgactgcac catcaggtcc agcgcgaatg cgattaaact gggaacggta tcgcggggtg
     1141 gattccgtca catccggatt ttacataaca gcgtctacga tacttatcgc tcggcccttg
     1201 ccctcgaggc ggtcgatggg gcagccatcc gggacctggt cgcggatagc ctgacggcgg
     1261 tgaataccgg caatgccctt ttcatgcgca tcggggcgag aagcggttcg cagcaaagta
     1321 gtctcgcgga tgtttccatc cggcatcttg acatccggat cgctgcgggc aaggcggatt
     1381 cgggatatcc ctatgaaggg ccggttgaag accaaccgag aaatatttca cctgtcgtcc
     1441 tggccggcct tcccggcgcg ttcttgcagg atattcaatt ggaggatgtg actgtccgtt
     1501 atccgggcgg cgggaagccg ttcactgtgg gccgcgatcc cgcgcggtcg gatagcgttc
     1561 ccgagttgcc ggcaaattat cccgagttct cgatgttccg ggaattgccg gcctgggggc
     1621 tttacatccg gcatgcgcgt gggatacgca ttcgaaatct gcgtctttat tgtgccgcca
     1681 aagattatcg caaggctata gtagtggatg atgtcaaggg ggcgacctgg gaggcgacgg
     1741 ttgtgagtga gcctgggaac acgaaggcat ttgggggaaa ttagccgttg aaaagtcctg
     1801 ggcctttgtg gtccacgggc cgccgccctt ctcagaatgt ctcgccgagg tttagatata
     1861 tgccggtgta gcccttgctg aaaccggcgt cgagagcgat gttcgtgccg gactttttgt
     1921 tgaattttat ccggaggccc gccccggcgg caggatgaaa ataggttaac tggtcagtcg
     1981 caggttcgct cacggcattg gcgttgaaga agacgacgaa tccgagcagc ccgtcggcag
     2041 tcagaacatg ccggtactcg gcctcgccgt ataagagggc cttgccccgg tagcggtttt
     2101 gaggaaagcc gcggccgctc cgttgctggt aagggtccca gccgatgctg ggcaggtcga
     2161 aatagggagc gtgggtttcc agcacggacc aataaaacac ccatagcgca aggatatcct
     2221 ggcgcttcag ggacaaggga acgtatttcc gtccgtccac gtagatagag ttccagggtg
     2281 tgctgctgcc ggtccacacg ggattcaccc ggtacacaac atgggcatag gatccgggca
     2341 gtggattttg agtatttgtc ctggtatcgt acaacagatt gaaggagatt ccggtagacg
     2401 tggattgttc gctatagccg gtgccgtagg ggtaatgggt gaagccttgc aggaggaggc
     2461 tgtcgccggg agcgtcgagg tcgtaacggt tatcgatcag cagtcccagg ccggcgaaga
     2521 gggacgtttc gatgcgtttc agcacggttt cataaacgcg gaaatactta tagtttatct
     2581 tgagttgcct gccgtattcc gtggaaccac cgagtcccca ggtatcctgc gggtagtaaa
     2641 ggaggcggat gtcgccaagg atgtcccagc tgttgcgggg cagccatagg ttggagcgaa
     2701 gcgcgaaccc gacccttccc tgcagtgcca cataaggcgc aaaagtcgcg gtggacaggt
     2761 tgcccgcact ccggttgaca agccggaaag cggcggttgt cgcggtgatg gtcaatcctg
     2821 cgggaccggc cgtctgggtg gagacgggta cgaatgaaaa ttggacgttc gttgccgtgg
     2881 cggagcgcgg atggctgcgg agatgaaaaa aagccctgcc gaggtcgatc agatcctttt
     2941 gttcagggcg ggtcgttgaa tcggtttgac caactgcctt gaggccgatg gtgcccgaca
     3001 atatgaggaa aagcgttcgc aagtcgatta aaccggaccg ttttaccggc ggtcccacac
     3061 ctatcgatga ggaagatacc ttatacggtt ggcctgaaac gccacattaa caaaaatata
     3121 atagttgtac cccggcaatc aactcccggc cggaagcagt tttgtgattt ttccaattaa
     3181 gtcctccagg gagaaaggtt ttcttagcca atcatcggcg ccagcttcga ttgccaattc
     3241 ttcgatgttg ttttcgctgg tgaagtacac aaccgggata tgcttacatc cggcgtccga
     3301 cttcaataat ttcgtcgcca tgatgccatc gaacattggc acgttgtgat ccataaaaat
     3361 caaccctggc tgaaacgctt ttacggtgtc cagcacattt tcgctccgct gaagcgtctt
     3421 gacctcgtag ccgaactttt ccaggatcag tctgatgatg tgaagatgat cattattgtc
     3481 atcgacgaca atgatgcggg cagcattatt catgggttca aactttttgc aatcgggggc
     3541 tacggctggt ttttctacca tgaagggatt aaaagatatg caatacgaat ttaagccatg
     3601 gccgacaata tttttcgtcg ggccgaagcc taaggcaatt cttagcagaa aaccattaga
     3661 ttacatgtca accggctggg gaaatttttc cccggcccgc gcggtaacct gccgagtgga
     3721 aggcatccaa aaacccgcgg aaaagcatta tattccgact cagaaactgc taaacaaatt
     3781 gaaacaagta cctggccgca agccagaaac catcgacgaa tacatcgatt cctacgacca
     3841 ggatatcgct gccatgcttc ggcaagttcg gaaagcgatc cgcgaggccg ctcctgcggc
     3901 aaccgaagtg atcagttacg gcatgcctgc ttttaagcag aacagggtac tcgtatattt
     3961 tgcggccaat aagaatcatt tgggatttta tccaaccgcc gagcccatcc gggtttttaa
     4021 gaatgagttg aaggactacg tgacctcgaa aggcgccatc cattttccgc tggataaagc
     4081 gctgccggtc tcattgatca aaaaaatcgt aaagttcagg gcagctgccg acagggctga
     4141 accaaattag gacaagggaa acgatattct tttgaaattc ccgtaatttg ttgttcctaa
     4201 ccactccgca tgaaaaaacg gacctttatc aaactctcct cggttatgat ggcgggaacg
     4261 gcgttgaccc cgttggttga tcttggacaa ggcggggaat tgaccaattg ggcgggaaat
     4321 attacctacg ggacgacgcg ggtgatgcgg gtcccttcgg ttcccgaggc cgccgaactg
     4381 gtcaagcgtt acgatcgcct ta
//
LOCUS       DATGKE010000696         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_152139, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000696 DATGKE010000000
VERSION     DATGKE010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152139"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..558
                     /locus_tag="VKU83_11820"
     CDS             115..558
                     /locus_tag="VKU83_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94296.1"
                     /translation="MADYIPDIRSLLPQGPPFIMVDRFLGSDGIVTRTSFLVTPGNPL
                     VEKGRFSAGGLIENLAQTAAAGAGWETMARAGEVVGKTAAGPRSGAVVSIDRLEIHDL
                     PAVGKELFTETSVTTRVGDIIVISGKISCGQSVIAVGEMKILAGV"
     gene            555..1670
                     /locus_tag="VKU83_11825"
     CDS             555..1670
                     /locus_tag="VKU83_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HLI94297.1"
                     /translation="MNDIFVVADNIFSPLGSSSAENFAQLRKGVSGVRRQERPDIADQ
                     PFFAALFAPAPTTGPLTRFEELLVSSMDAALRDAGLDPADPRTILIISTTKGNIPLLE
                     AGIGDPARLSLTGSAQVVAARFGFVNRPIVISNACISGILALIAGMRLIRSGKYDTAV
                     VTGADLISKFVLSGFASFQAVSSGPCRPFDEARNGINLGEGAGTVVLSGKDRYRGRLR
                     LTGGAVSNDANHISGPSRTGRELAAAIDRALGEASCGPADIDFISAHGTATIYNDEME
                     ARAITLAGLQSVPVNSLKGSYGHTLGAAGLIETIVTLQSMREELLLPSAGFSRSGVST
                     PINVCTSLQPGPLRHCLKTGSGFGGCNAALVISKSEN"
     gene            1671..2291
                     /locus_tag="VKU83_11830"
     CDS             1671..2291
                     /locus_tag="VKU83_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94298.1"
                     /translation="MEYITASCVIRDCQVWQNGNPVLDYGRESLADFLLAAYQQYGAQ
                     YPKFYKMDHLSKLGWLAAEVLMGNGFRGKGYLPEEVAVVLSNASSSLDTDYRYFGSLA
                     AVPSPALFVYTLPNIMIGEICIRHTWKGENAFFVFESFDAGFIAGYVCELLRQDRARA
                     CVCGWVEVLNERHSAALFLVEKELGELPFTAENLTAIMNMQNSRHG"
     gene            2284..>2692
                     /locus_tag="VKU83_11835"
     CDS             2284..>2692
                     /locus_tag="VKU83_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94299.1"
                     /translation="MDKVSPVAIAGTGIVSAIGDNRAECLAALAAGCAGIDDIRLLDS
                     AHRGRLPVAEVKHPDAELAAMAGVDEAAGRTALLALIAAREALESARLPDLGKWRTGL
                     ISANTVGGMDRTEQFFAQGARGKLRDVVRHECGW"
BASE COUNT          499 a          785 c          867 g          541 t
ORIGIN      
        1 tgaaagtgct gatcccggtc aggcggttcg gcttgccgga agaagtagcg tacgcggtag
       61 cattcctcgc ctcgccgcgc gcatcgtata taacagcgga agtgctcagc atcaatggcg
      121 gactatattc ctgacatccg gtccctgctg ccgcaggggc ccccgtttat catggtggac
      181 cggttcctgg gatcggacgg aatcgtgacg cggacgagtt tccttgtcac cccgggcaac
      241 cctttagtgg aaaagggcag gttcagcgcc ggtggcctga tcgaaaacct cgcgcagacc
      301 gccgcagccg gcgccggatg ggagaccatg gcgcgtgccg gcgaggttgt cgggaagacc
      361 gcagcaggtc cgcgaagcgg cgccgtggtt tccatcgatc gtctcgagat ccatgacctg
      421 cccgctgtag gaaaggaact ctttacggaa acctcggtca cgacgcgggt cggcgatatt
      481 atcgtaattt caggcaagat cagctgcgga caatcggtta tcgccgtggg tgagatgaaa
      541 atccttgctg gagtatgaac gatatcttcg ttgtggcaga caatattttt tcgccgcttg
      601 gcagcagttc ggcggaaaat ttcgcgcagc tgcggaaggg agtttccggg gtcaggcgac
      661 aggaacggcc ggatatcgcc gaccagccat tctttgctgc tttgttcgca ccagcgccca
      721 cgaccggccc gctcacccgt tttgaggaac tgctggtttc ttccatggac gccgccttgc
      781 gcgatgcggg gctcgacccg gctgatccca ggacgatcct gatcatttcc acgacaaagg
      841 gcaatattcc gctcctggaa gccgggatcg gggaccctgc ccgcctgtca ttgaccggct
      901 ccgcgcaggt cgtcgccgcc cgttttgggt ttgtgaaccg gcccatcgtt atctcgaacg
      961 cctgcatttc cggcatcctc gccctgatcg cggggatgag gctgatccgg tcgggaaaat
     1021 atgacacggc ggtcgttacc ggggctgacc tgatctcgaa attcgtgctt tccggcttcg
     1081 cgtccttcca ggccgtcagc tccggtccct gccggccctt cgatgaggcg cggaacggca
     1141 tcaacctcgg tgagggtgcc ggaacggtcg tcctttcagg aaaggaccgg taccggggca
     1201 ggctgcggct gaccggcggc gcggtgagca atgacgccaa tcatatctcc gggccctccc
     1261 gcacgggcag ggaactcgcg gcggcgatag accgggcgct cggcgaggca agctgcggtc
     1321 cggccgacat cgactttatt tcggcgcacg ggaccgctac gatctataac gatgaaatgg
     1381 aggcgcgggc gatcaccctc gcgggtctcc agtcggtccc ggtcaacagt ctgaaggggt
     1441 cgtacggcca tacgctgggc gcggcaggcc tgatcgagac cattgttacc ctccagtcca
     1501 tgcgcgaaga gttgctgttg cccagcgccg gttttagccg cagcggcgtg agcacgccga
     1561 ttaacgtctg tacatccctg caacccggcc cgctgcggca ttgtcttaag acgggctccg
     1621 gttttggcgg ctgcaatgct gccctggtca tcagcaaatc cgagaattga atggaatata
     1681 tcaccgccag ctgtgtcatt cgggattgcc aggtctggca gaacggaaat ccagtcctgg
     1741 attacggccg ggaaagcctg gcggatttcc tcttggcggc ataccagcaa tacggcgccc
     1801 agtacccgaa gttttacaag atggatcatc tcagcaagct gggttggctt gccgcggagg
     1861 tactgatggg aaatggattc cgggggaaag ggtacctgcc ggaagaagta gcggtcgttt
     1921 tgtccaacgc cagctcgagc ctggacacag attataggta tttcggttcg cttgcagcgg
     1981 tgcccagccc ggcgctgttt gtctacaccc tgccaaatat catgatcggc gagatctgca
     2041 tccggcatac atggaagggc gagaatgcat tctttgtctt cgaatccttc gacgcagggt
     2101 ttatcgccgg atatgtctgc gaactgcttc ggcaagaccg ggcccgggcc tgcgtctgcg
     2161 gctgggtgga ggtgttgaac gagcgtcatt ccgcggcgct gttcctggtc gaaaaggagt
     2221 tgggcgagtt gccgttcacc gccgaaaatc tgaccgccat tatgaacatg caaaattcgc
     2281 gtcatggata aagtctcgcc ggtggcgata gccgggacgg ggatcgtttc cgcgatcggc
     2341 gataaccgcg ctgaatgtct cgcggcgctt gcggccggct gcgcgggaat cgatgacatc
     2401 cggctgctgg attcggcgca ccgcggccgg ctgccggtcg ccgaggtaaa acatcccgat
     2461 gctgaactgg ccgcgatggc aggagtggac gaagccgcgg gcaggaccgc tttgctcgca
     2521 ctcatcgctg cgcgggaggc gctggaaagc gcccggctcc ccgatctggg caaatggcgc
     2581 accggcctga tctccgccaa taccgtcggc ggcatggaca ggaccgagca attcttcgcg
     2641 cagggcgccc ggggcaagct gcgggatgtg gtccggcatg aatgcggctg ga
//
LOCUS       DATGKE010000697         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_957317, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000697 DATGKE010000000
VERSION     DATGKE010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957317"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="eno"
                     /locus_tag="VKU83_11840"
     CDS             complement(<1..410)
                     /gene="eno"
                     /locus_tag="VKU83_11840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF015885.4"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLI94300.1"
                     /translation="MSYIADIHARQILDSRGNPTVEVDVVTENNFRGRAAVPSGASTG
                     MHEAVELRDGDKKVFGGKSVLKAVANVNDIIADQLIGFPVNEQASLDARLIDMDGTDN
                     KGKLGANALLAVSMAAAKAAAQESKLPLYRYLGGV"
     gene            complement(448..1356)
                     /gene="gldA"
                     /locus_tag="VKU83_11845"
     CDS             complement(448..1356)
                     /gene="gldA"
                     /locus_tag="VKU83_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HLI94301.1"
                     /translation="MSIEVKDLVKVYGGQKAVDAISFTVGKGQIAGFLGPNGAGKSTT
                     MKILTGYLGQDAGEALVCGISVREHPLETKKKIGYLPEANPLYPDMFVREYLDFIADI
                     QHVPAKRQRIETVIHLVGLTAESKKKLGQLSKGYRQRVGLAAALLHEPEVLILDEPTS
                     GLDPNQIVEIRDVIRRLGKDKTILFSSHILQEVEALCDRVIIINNGRLVADSPLSRLR
                     EQTAGAGTIRVMFRETPDAAALEQLDSVSAVNRCSDGHWELHTASPDLVKKQLMELAL
                     QNNWNIVSLQSENRSLEDIFRQLTNN"
     gene            complement(1365..>2853)
                     /locus_tag="VKU83_11850"
     CDS             complement(1365..>2853)
                     /locus_tag="VKU83_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI94302.1"
                     /translation="AGTLLEWYDFYLYLNLATVIASRFFPGSHPGAALLFTLGSFAAG
                     IIMRPVGALLFGRRGDRAGRKPAWLMTLTLMGLSTFATGLVPGYDRIGIAAPAVILAL
                     RLLQGLAIGGGYGGATVYVAEHSPAHRRGYFTSWIQGSTMAGLLVSLAVILLSRYALD
                     PDRAVSLEKFDAWGWRIPFLASLILLPLAAWFRVKLQESPVFRQFTPEGNVSPDPLKA
                     SLAPGNAKRLLAAIFGLTMGQGVAFVTGGLYAQTFLTDTCRIDIDQVRSMVLIALALS
                     TPFYVIGGRWSDRIGRKWPMLGGLLIAVASWALLFREMPPIAGTSGRILLPDKTEIRN
                     SVAFIGRTRAMLRTSATISYFQDGMQVVESRTDTVFADGQHSAATKPSICRTLPGRDF
                     WKIIALLFPTALGIAISLGPAGAFLSEVFPAGTRCTSVSVSHEAGIGIFGGLTAIVTL
                     FLAGGGPGRELAGLWYPTGVGIICLLTGILFLPDGRKGSHLEIAP"
BASE COUNT          600 a          842 c          816 g          595 t
ORIGIN      
        1 acgcccccca gataacggta tagaggcagc ttgctttcct gcgccgcggc cttggccgca
       61 gccatgctaa ccgcgagcag tgcgttggcg cccagcttcc ctttattatc ggtgccgtcc
      121 atatcgatca gccgcgcatc gagcgacgct tgttcgttga caggaaaacc gatcaactgg
      181 tcggcaatga tatcgttgac gttggctacc gctttcaaaa cactttttcc gccaaatacc
      241 tttttatcgc cgtcgcgcaa ttcgaccgct tcgtgcatgc cggttgaggc gccactgggc
      301 acggctgccc ggccgcggaa attgttttct gttacgacgt ctacttctac ggttggatta
      361 ccccggctgt ctaagatctg gcgggcgtgg atgtcagcga tatagctcat gatggtcgaa
      421 tttgcgcgga cccgcggtcc gcatttttta attatttgtc aattgccgga agatgtcttc
      481 caggcttcgg ttttcgcttt gcaaagaaac aatgttccaa ttgttctgca aagcaagctc
      541 catcaattgt ttcttaacga ggtcgggaga tgccgtgtgc aattcccagt gtccgtcgga
      601 gcatctgttc accgcactga cggaatccag ctgctcaagc gctgcggcgt cgggcgtttc
      661 gcggaacatc acgcggatgg tgccggctcc ggctgtttgt tcccgcagcc ggcttagcgg
      721 gctgtccgcg accagccggc cgttattgat gatgatcacc cggtcgcaaa gcgcttccac
      781 ttcctgcagg atatgtgagg agaacaggat cgttttatct ttacccagcc tcctgatcac
      841 gtccctgatc tcgacgatct ggttaggatc gaggccgctg gtcggttcat cgaggatcag
      901 cacctcgggc tcatgcagca aagcagctgc cagccccacc cgctggcggt atccttttga
      961 taactggccc agcttttttt tgctttcggc ggtcaggcca acgagatgga tgacggtttc
     1021 aatccgctga cgttttgccg gaacgtgctg aatgtcggca ataaaatcca ggtattcacg
     1081 aacgaacatg tcagggtaga ggggattggc ctccggcaga tagccgatct ttttttttgt
     1141 ttccagcggg tgctcgcgga cagaaatgcc gcaaaccagc gcttcgccgg cgtcctggcc
     1201 gaggtagccg gtcaggatct tcatcgtcgt gctcttcccc gcaccgttgg gaccgaggaa
     1261 accggcaatc tgtccttttc caaccgtaaa ggagatcgcg tcaactgcct tttgcccgcc
     1321 atataccttg acaaggtcct ttacttcgat tgacatgcgc gaatttacgg tgcgatttcc
     1381 aaatgcgacc cctttcttcc gtccgggagg aaaaggatgc cggtcagcag gcagatgatc
     1441 cccactccgg tcggatacca tagcccagcc agctccctgc cgggacctcc cccggccaaa
     1501 aacaaggtga caatagcggt tagtccgccg aagatgccaa taccggcttc atgcgaaacc
     1561 gaaacggatg tacagcgggt gccggcggga aacacttcgc tgaggaaagc gccggccggt
     1621 ccgagggaga tcgcaatgcc cagcgctgtc gggaaaagga gagcgatgat cttccaaaaa
     1681 tcgcgtccgg gaagggtccg gcatatcgac ggctttgtcg cggcgctatg ttggccgtcg
     1741 gcgaagacag tgtccgtcct tgactcgaca acctgcattc cgtcctggaa atagctgatc
     1801 gtcgcggacg ttctcagcat ggccctcgtc ctgccgataa aagctacgct gtttctgatc
     1861 tccgtcttgt caggcagcaa aatccgccct gaagttccgg caatcggggg catttcccga
     1921 aaaagcaagg cccaactggc gacggctatt aacaatcccc caagcatcgg ccatttccgg
     1981 ccgatccggt cgctccatcg cccgcctatg acatagaagg gcgtggaaag ggcaagggca
     2041 atcagtacca tcgatcttac ctggtcaata tcgatgcggc acgtgtccgt caggaatgtc
     2101 tgtgcgtaaa ggccaccggt gacaaaagcg acgccctgcc ccatggtcag gccaaaaatt
     2161 gccgccaaca gccgctttgc attgcctggc gcaaggctcg ccttcagcgg atccggcgaa
     2221 acgtttcctt ccggcgtgaa ctgccggaag accggcgact cctgcagttt taccctgaac
     2281 catgcggcca aaggcaggag gatcagggag gccagaaaag ggatccgcca accccaggcg
     2341 tcgaatttct ccaggcttac ggcacggtcc ggatccaggg catacctgct gagaaggatt
     2401 acggcgaggc tgaccagaag gccggccatg gtgcttccct gaatccacga cgtaaaatag
     2461 ccccggcggt gcgcagggga atgttccgcg acatagacgg tcgctccgcc gtaacccccg
     2521 ccgatggcca gtccctgcag taaccgaagc gccaggatga cagcgggggc cgcgatgccg
     2581 atccggtcat acccgggaac caggccggtc gcaaaggtgg aaaggcccat gagggtgagg
     2641 gtcatcaacc aggcgggctt tcggcctgcg cggtcgccgc gtcgcccgaa cagcagggcg
     2701 ccgacaggtc gcatgattat tccggcggca aaggaaccaa gcgtgaacaa cagcgccgcc
     2761 cccggatggg atccggggaa gaatcgcgac gcaatcaccg ttgccagatt caggtaaaga
     2821 taaaagtcgt accattccag gagggtgccg gcg
//
LOCUS       DATGKE010000698         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_156465, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000698 DATGKE010000000
VERSION     DATGKE010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156465"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VKU83_11855"
     CDS             <1..320
                     /locus_tag="VKU83_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HLI94303.1"
                     /translation="TGIAIVRRAIEEPLRQIVRNCGLEGSIVVQQVRQGKNDDGFNAR
                     TEKYEPLLKAGVIDPTKVTRIALENAASIAGMLLTTECVVSDKPKKEIAPAPATPGMG
                     MDY"
     gene            542..1957
                     /locus_tag="VKU83_11860"
     CDS             542..1957
                     /locus_tag="VKU83_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLI94304.1"
                     /translation="MKIALALAVVTCFATRLPAQTYSAEVEKYIEHKEPVIAFVHALV
                     VDSKNAASRAGQTVVVRNGIIVAVGDDKKISIPADAKVIDLSGKALLPGFVLMHEHMY
                     RSDFSLSPLYLHVQQLPVTFPRLYLACGATTIRTAGSVEPYSDLNLKRDIDAGKIIGP
                     EMDVTAPYLEGKGSVFPQMHELKDAAEAKPFVDFWAGEGATSFKAYNTLTKQELASAI
                     REVHARKLKITGHLCSVTYREAAEMGIDQLEHGFLASTDFVPGKKENACPFAPNPFGG
                     LTVDSPEVKDFIRLLIRKKVIITSTLAVFEGAATLDTTLRPEVLEAMSPDTRKVFLQE
                     YNKARQPAFNQAIVEDMRMEKSFADSGGLLTVGTDPTGNGGTLAGYGSQQSIELLVKA
                     GFNPLEAIRIATYNGALAMGLEKKIGSIEVGKQADLIVLDADPSQNIDNIEKVSLVFK
                     KGIGFNSPMLFHSVMGKVGRY"
     gene            complement(1979..>2472)
                     /locus_tag="VKU83_11865"
     CDS             complement(1979..>2472)
                     /locus_tag="VKU83_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94305.1"
                     /translation="PGNDFSEVEAVGYHVGLETWKLLAAAGGQGPQAGEAGAAHAVRS
                     VHVMTTTLNFPGKKGWKDRFPETSEPTGSDVPVRITGLKLDELVFCGISGELMTEMGM
                     AIKAASPYTSTIIVTHCNGASGYICTDKAFSEGGYEPKVSHLMPGAEKPLVAKCLELI
                     RRF"
BASE COUNT          570 a          716 c          687 g          499 t
ORIGIN      
        1 cgacgggcat cgccatcgtt cgaagggcta tcgaggaacc gctccgccag atcgtgcgca
       61 attgcggcct ggaaggaagc atcgtggtgc agcaggtccg ccagggcaag aacgacgacg
      121 ggtttaatgc ccgcaccgaa aagtatgagc ccctgttaaa ggcgggggtc atcgatccca
      181 ccaaagtcac ccggatcgcc ctcgaaaatg ccgcttccat tgccggaatg ctgctgacga
      241 cggaatgcgt tgtttcggac aagccgaaaa aggagattgc tcccgcgccg gcaactcccg
      301 gaatgggcat ggattattaa ccagcccacc cacccaaggc gcattactat atacaagcga
      361 atcccgttgc tccggcaatc cgcgccggca acggggttcc accatttagg gcaggagcgg
      421 aaagctcatg ggctgtggag ctcaaataaa atgatccgga aaaaagtttg cgaatccggc
      481 caatgcacct atatttgcaa ccgaacggtt tcctttttta agtcaccaat tttaaatgct
      541 gatgaaaatc gccctcgcgc ttgcagtagt cacctgcttt gctacccgcc tcccggcgca
      601 gacctattcc gctgaagtgg aaaaatatat cgagcacaaa gagccggtta tcgcctttgt
      661 tcacgccctg gtggtcgatt caaaaaatgc agcttccaga gccggccaga ccgtcgtcgt
      721 tcgcaatggc atcatcgtgg ccgtgggcga cgataaaaag atcagcatcc cggcggacgc
      781 caaggtcatc gatctgagcg gcaaagcgct cttgcccggt tttgttctga tgcatgagca
      841 catgtatcga tcggacttta gcctgtcccc gctctatctt catgtacagc aacttccggt
      901 aacctttcca agattatacc tcgcttgcgg ggccacgaca atccgaaccg cgggcagcgt
      961 cgagccgtat tccgacctta acctgaagcg ggatatcgac gcggggaaga tcatcgggcc
     1021 ggagatggac gtaacggcgc cctacctgga agggaaggga agtgttttcc cgcaaatgca
     1081 cgaactaaag gacgccgcgg aagcaaagcc atttgtggat ttttgggcgg gcgaaggcgc
     1141 cacttccttt aaggcctaca acacgcttac caaacaggag ctggcatcgg cgatccgtga
     1201 agttcatgcc agaaagctca aaataacggg ccatttgtgt tcggtaacgt accgggaagc
     1261 ggcggaaatg ggaatcgacc aactggaaca tggattcctg gcttccaccg attttgttcc
     1321 gggaaaaaaa gaaaatgcct gcccttttgc gcccaacccg tttggcggtt tgactgtcga
     1381 cagccctgaa gtcaaagact tcattcgcct cctcatccgg aaaaaagtga tcataacgtc
     1441 caccctggcg gtcttcgaag gtgcggccac gctggatacg accctccggc cggaagtgct
     1501 ggaagccatg tcccccgata cccgcaaggt tttccttcag gaatacaaca aagcgcgcca
     1561 acctgccttc aaccaggcga tcgtcgaaga catgcgcatg gaaaaaagct ttgcggattc
     1621 gggcgggctc ctgacggtcg gaaccgaccc gactggaaac ggcggcacgc tggcgggcta
     1681 tgggtcgcaa cagtcgatcg agttgcttgt caaggccggg ttcaaccctc ttgaggcgat
     1741 ccggattgcc acctacaacg gcgcactggc gatgggattg gaaaaaaaga tcggctccat
     1801 cgaagtgggc aagcaggccg atctcatcgt gctggatgct gacccatcgc agaacatcga
     1861 taacatcgaa aaggtgagcc tggtctttaa aaaaggaata ggattcaatt cacccatgct
     1921 gttccattcc gtcatgggca aggtcggccg ctattgatgg ggcgcgcgcg ccatcgcctt
     1981 aaaacctcct tatgagctcg aggcatttgg ccaccagcgg cttttcggct cccggcatga
     2041 gatgactcac cttgggttcg taaccgccct cgctaaacgc tttatcggtg cagatatacc
     2101 cgctggcgcc gttgcaatgc gtgacgatga tggtggaagt ataaggcgag gcggccttga
     2161 tcgccattcc catctccgtc atgagctcgc cggaaatccc gcagaacacc agctcgtcca
     2221 gcttgaggcc ggtaatccgc acgggcacgt cggaaccggt gggttcggag gtttccggga
     2281 accggtcctt ccagcccttt tttccgggaa agttcagcgt ggtggtcatg acgtggacgg
     2341 agcgcacggc atgcgccgcg ccggcttcac cggcctgcgg gccctgcccg cctgccgcgg
     2401 ccagcagctt ccatgtctcc aggcccacat gatacccgac cgcttctact tcggagaaat
     2461 cgttacccgg cc
//
LOCUS       DATGKE010000699         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1120728, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000699 DATGKE010000000
VERSION     DATGKE010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120728"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VKU83_11870"
     CDS             <1..1200
                     /locus_tag="VKU83_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94306.1"
                     /translation="CSGIDPDILCKLMGTKMLTGKSILVISPQSWGKMSLSKHHYAIE
                     LAKRGNRVYFLNPPGKRQDRKKGAVTIVPAEATPGLWLIEHRLWFPYSLKFHALPLFH
                     ALMRFHVARIMRQIGRPVDLVWSFDLGNLYPFRLFGRRPHKIFHPVDEPLNQVAIDSA
                     KDAEILFSVTREIVDKYKAFPAPGYFINHGLAEEFLTKGPAAWEKSLPLRVGLSGNWT
                     RPDIDTACLLEIIRGQPAVIFEFWGSYVSADSNIGGNSDPAIENFLRELQHAPNVILH
                     GPVPSARLAEELRRVDAFLICYDVQKDQSKGTNYHKVMEYLSTGKVIIANNITTYRDR
                     PDLVQMVPERDSNHSLPRLFDTVIGNIEKYNTESLRQARHSYAVDNAYQKQLDRIAGI
                     LESTLHE"
     gene            1193..2374
                     /locus_tag="VKU83_11875"
     CDS             1193..2374
                     /locus_tag="VKU83_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLI94307.1"
                     /translation="MSEDRKITYILSHPIQYFAPLLGALAKVTDLEVYYLSDASIKGQ
                     KDAGFGKPIAWDTALLDGYPAFFIRNFGRRGNPDNHFWDVFNPGVVGVIRKRKRSIVI
                     VNGWTYSSTLLAILSSKLLGRKVWLRAENPLNQELRKSRRVLFLKRLFLKHLLFRYFI
                     DKCLYIGTESRKFFGYYGVPADRLVYTPYAVDNAFFSEAYQHWKGRTVELKQKLGLPP
                     NKKIILFVGKYTPKKRPLDLLEAFRQLQPADHLLVMVGEGELRPEMEKFTATRALKEK
                     VVFTGFVNQSRIPLYYSVADVFVMCSGMGETWGLAVNEAMNFAKPVIVSDTCGCSADL
                     VRHGGNGFIFEEGNIGQLRGYLQQVLADGPFAEKAGLESMEIVREFSIQRIVQNVSQA
                     I"
     gene            2371..3393
                     /locus_tag="VKU83_11880"
     CDS             2371..3393
                     /locus_tag="VKU83_11880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI94308.1"
                     /translation="MRVLIVGSDEVWSLEKYYGKNLGNAGVGVRSFPAQSIFYGYYHK
                     SIIHKLLFKSGLSPVGRMIETQMRAVVADCQPDIVWIFKGMELSPDLLRWIKGKGIRL
                     VNYNPDNPFLFSGRGSGNKNVTDSIGLYDLHFSYDQSICRQISKDYNIPCRILPFGFE
                     LAEGLYAECEEQVETVKLCFLGNPDRQRAAFISQLAEAFPIDVFGYHWESFVKHDRIT
                     IFPPVYGEAFWKTLRRYRVQLNLMRAHNLHSHNMRTFEIPGVGGIGLFPRTPDHEAFF
                     EAGKEVFLYDDMAGCKQQAAVLLKMTQGEAAEIRRAARRRSVGSGYSYQDRALQALTE
                     MKKLLA"
BASE COUNT          845 a          893 c          981 g          837 t
ORIGIN      
        1 tgtagcggga tcgatccgga cattttatgc aaactgatgg gtacaaagat gcttaccggt
       61 aaatcgatcc tggttatttc cccccaaagc tgggggaaga tgtcgctttc aaagcatcat
      121 tatgcgatcg aattggcgaa aagaggcaac agggtctatt tcctgaaccc gccgggaaaa
      181 aggcaggacc ggaaaaaggg agccgtcacg atcgtccccg ctgaggccac cccgggtctc
      241 tggttgatcg aacaccggct atggttcccc tatagcctga agtttcacgc cctgcctttg
      301 ttccatgccc tgatgagatt ccatgtagcc cggatcatga ggcagatcgg gcgcccggtg
      361 gatctcgtct ggtccttcga tcttggaaac ctataccctt tccgcctttt cgggcgccgg
      421 ccccataaaa tatttcaccc ggtagacgag cctcttaacc aggtcgccat tgattcggca
      481 aaggatgccg agatcctttt ttcggttacc cgggagattg tcgacaagta caaggccttt
      541 ccggcgccgg gatattttat taatcatgga ttggcggaag aatttttgac aaaagggccc
      601 gccgcctggg aaaagtcgct gcccctgcgg gtgggccttt ccggaaactg gacgcggccg
      661 gatatcgata ccgcctgcct gctggaaatc attcgcgggc aaccggcagt catctttgaa
      721 ttctggggca gctacgtgtc ggccgattcg aacatcggcg ggaatagcga tcctgctatt
      781 gaaaactttc tccgcgaatt gcaacatgct cccaacgtca tcctccacgg gccggttcct
      841 tcggcccgct tggcggaaga gctgcgccgc gtcgatgcct tcctgatttg ctacgacgta
      901 cagaaagacc agagcaaggg aacgaactac cacaaagtaa tggaatacct gagcaccggc
      961 aaggtcatta tcgccaataa catcactacc tatcgtgaca gaccggatct cgtgcagatg
     1021 gtgcccgaaa gggacagcaa tcacagcctt ccgcgattgt tcgataccgt catcggcaat
     1081 atcgagaaat acaataccga gtcattgcgg caggcaagac attcctatgc cgtggataac
     1141 gcctatcaaa aacagttgga ccggatagcg ggcatcttag aaagcacact acatgagtga
     1201 agaccggaag atcacctata tccttagtca tcccatccag tattttgctc cattgttggg
     1261 agcgctggct aaagtaaccg acctcgaggt atattattta tcggatgcga gtatcaaagg
     1321 gcaaaaagat gcgggtttcg gtaaacctat tgcctgggat acagcgctgc tggacgggta
     1381 tcccgccttt tttatcagga attttggccg aaggggcaac ccggacaatc atttttggga
     1441 tgttttcaat cccggcgtcg ttggcgtgat caggaaacgc aaacgatcca ttgtcatcgt
     1501 caacggctgg acctacagtt cgaccttgct cgcgatcctt tcgtcgaagc tcctgggcag
     1561 gaaggtctgg ctcagagcgg aaaatccgct gaaccaggaa ttacggaagt cccggcgggt
     1621 cctgttcctg aagcggcttt tcctcaagca tctcctgttc cgatatttta tcgataaatg
     1681 cctttacatc gggaccgaga gcaggaagtt cttcgggtac tatggcgttc cggcagacag
     1741 gctcgtctat acgccatatg cggtggacaa tgcctttttt tctgaggcat atcagcattg
     1801 gaaggggcga accgtcgagc tgaagcaaaa attgggcttg ccgccaaata agaaaataat
     1861 cctctttgtc ggcaaataca cgccaaagaa gcggccgctg gacctcctgg aggcatttcg
     1921 gcagctgcag ccggcggatc atctgttggt aatggttggc gaaggggaac tccggccgga
     1981 aatggaaaaa ttcaccgcga cccgggcgtt gaaagaaaaa gttgtcttca cggggttcgt
     2041 aaatcagtcc cggatcccgc tctattattc ggtagcggat gtttttgtca tgtgttcggg
     2101 aatgggcgag acctggggac tggctgtcaa cgaagcgatg aacttcgcga aaccggtaat
     2161 cgtttccgat acctgcggct gcagcgcgga cctcgtgcgt catggcggaa acgggttcat
     2221 tttcgaagaa gggaatatcg gacaactgag ggggtatctg caacaggtcc tcgccgatgg
     2281 gcctttcgcc gaaaaggccg gcctggaatc catggaaata gtcagggaat tttccattca
     2341 gaggatcgtt caaaatgtca gccaggcgat atgagagtat taattgtggg ctctgatgaa
     2401 gtctggtcgc tggaaaagta ttatggcaaa aacctgggga atgccggcgt cggcgtccgc
     2461 agtttccctg cgcaaagcat cttctacggc tattaccata agagcatcat ccataaactc
     2521 ttgttcaaat cgggcttgtc gccggtcggc aggatgattg aaacccaaat gcgggcggtc
     2581 gtggcggatt gccagccgga tatcgtgtgg atctttaagg gcatggaatt atcgccggac
     2641 ctattaagat ggatcaaggg caaaggcatc aggttggtga actataaccc cgataaccct
     2701 tttctattca gtggaagagg cagcggcaat aaaaatgtga cggactccat cggtttatat
     2761 gatcttcatt tttcgtatga ccaatccatt tgccggcaga tcagcaagga ctataacatc
     2821 ccttgccgga tccttccctt tggattcgag cttgccgagg gtctgtatgc ggagtgcgaa
     2881 gaacaggtcg agaccgtgaa gctctgcttt cttggcaacc ccgacaggca aagagcggct
     2941 tttatcagcc aactggcgga agcgtttccc atcgatgtgt tcggatacca ctgggaaagc
     3001 ttcgtgaagc acgacaggat caccattttt ccgcctgtgt acggcgaagc attttggaag
     3061 accctgcgcc gctacagggt gcaattaaac ctcatgcggg cccacaacct gcattcccac
     3121 aatatgcgta cttttgaaat cccgggcgta ggcggcattg gacttttccc gcggacgccg
     3181 gatcacgagg cttttttcga agcgggaaag gaggtttttc tatacgacga tatggccgga
     3241 tgcaagcagc aggccgctgt gttgttgaag atgacgcaag gcgaggccgc cgaaatccgc
     3301 cgggccgcac ggcgcaggag cgtcggttcc gggtacagct atcaggatag ggcattgcag
     3361 gccctgacgg aaatgaaaaa actactggca tgaaaaggcg ggagagtccc ctgctcgttt
     3421 attattattc catctcggtg attttcctgc tgatactgga atgggtcacc gattggtatt
     3481 ttcccctggc gttcgccctg atcgtgctta ccgtggtcat gatcctggac agattgggga
     3541 aaggtatcgt tttgcg
//
LOCUS       DATGKE010000700         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_470973, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000700 DATGKE010000000
VERSION     DATGKE010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470973"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VKU83_11885"
     CDS             <1..1161
                     /locus_tag="VKU83_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLI94309.1"
                     /translation="QFMPPMAMNVVEKDLAVRLKDHYKNSRHMIIGRSANITEPLPGR
                     TNCQYRDKCWLGCPFGGYFSTQSSTLPAAMATKKLTLRPWSIVTQVLYDKDKKRAIGV
                     EVLDAETNKTYHYKSKIVFLNASTINSAWLLMNSATDVWPDGLGSSSGELGHNLMDHH
                     LDVGAYGRVEGYEDKYYYGRRANGIYIPRFRNLFGDKRDYVRGFGYQGGAGRDGWDRE
                     IAELSIGADLRAALSEPGGWTMGVGGFGETLPYHDNKVTLDKTRKDKWGLNIPAVDAV
                     IRENEYKMRKDMQADAVEMMTAIGLKDVQGFDNNPVLGRGIHEMGTCRMGKDPKTSVL
                     NKWNQVWDAPNVFVTDGAFMTSTNCVNPSLTYMAFTARAANHAVDELKKMNL"
     gene            1172..1714
                     /locus_tag="VKU83_11890"
     CDS             1172..1714
                     /locus_tag="VKU83_11890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLI94310.1"
                     /translation="MQRREALQMVAVLMGGTVIGADAFLSGCKTAPRKNGLFYDTDVA
                     MMDEVGETILPAGAGVPGAKEAGIGEFMKVIVTDCYTPEDQDVFVKGLQALRDKKFMD
                     LTPGQRHDWLVQLDKESRDPKYNGNGKQQHYFKMLKQLTLWGYFTSEAGATKALRYIE
                     TPGHYDGDYPYKKGDKAWAL"
     gene            1854..>2070
                     /locus_tag="VKU83_11895"
     CDS             1854..>2070
                     /locus_tag="VKU83_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94311.1"
                     /translation="MIRPLLYVVILFVCGSWMRQTDPWTPAQLMEPADLAAALNHSTN
                     RLPLLVCVGPSGIIKGSIETGPAHEPQN"
BASE COUNT          499 a          549 c          620 g          402 t
ORIGIN      
        1 cagttcatgc cgccgatggc aatgaatgtc gtggagaagg acctggccgt ccggctgaaa
       61 gaccactata agaacagccg gcacatgatc atcgggcgat cggcgaacat cacggaaccg
      121 ctccccggcc ggaccaactg ccaataccgg gacaagtgtt ggctgggctg cccgttcggc
      181 ggctatttta gtacccagtc gtctaccctc ccggcggcga tggcaacgaa gaagctgacg
      241 ttgcgaccct ggtcgatcgt gacccaggtg ctctatgata aggataagaa acgggcaatc
      301 ggggtggaag tgctcgatgc cgaaaccaac aagacctacc attacaaatc gaagatcgtc
      361 ttcctgaatg cctctaccat taacagcgcc tggctgctga tgaattccgc tacggatgtt
      421 tggcccgacg gactgggatc gagcagcggc gagctgggcc ataaccttat ggatcaccac
      481 ctggacgtgg gcgcctacgg ccgcgtggag ggatatgaag acaaatatta ctatggccgc
      541 cgcgccaacg gcatctatat cccgcgattc cggaaccttt tcggcgacaa acgcgattat
      601 gtcaggggat ttggatacca gggcggcgcc ggccgggacg gttgggaccg cgagatcgcc
      661 gaactgagca ttggcgccga cctgcgcgcc gcgctctccg agccgggcgg ctggaccatg
      721 ggggtgggcg gtttcggtga aacgctcccc taccacgaca ataaggtaac actcgacaag
      781 acgcggaagg acaaatgggg cctcaatatc cccgccgtcg acgcagtgat ccgggagaac
      841 gaatacaaaa tgcgcaagga catgcaggcc gatgcggtcg aaatgatgac ggcaatcggg
      901 ctcaaggatg tgcaaggctt tgataacaat cctgtccttg gccgcggcat ccatgagatg
      961 ggcacctgcc gcatggggaa agacccgaaa acttccgtat tgaacaaatg gaaccaggtc
     1021 tgggacgcgc cgaacgtatt tgtgaccgac ggagctttca tgacctccac caactgtgtc
     1081 aatccttccc tcacgtatat ggccttcacg gcccgggcag cgaaccatgc ggtggacgaa
     1141 ctcaaaaaaa tgaacctcta aatttctcaa gatgcaaaga agagaagcgt tacaaatggt
     1201 agccgtcctg atgggcggaa cggtgatcgg cgcggatgct ttcttgtccg gctgtaaaac
     1261 agctcccagg aagaatggtc ttttttatga taccgatgtc gccatgatgg atgaggtcgg
     1321 tgagaccatc ctgccggcgg gcgccggagt gccgggggca aaagaagcgg gcatcggcga
     1381 attcatgaag gtgatcgtga cagactgcta cactcccgaa gaccaggacg tctttgtgaa
     1441 aggcttgcag gcgctgcgcg acaagaaatt catggacctg acccccgggc agcgccacga
     1501 ttggctggtt cagctggata aggaatcccg tgatccgaaa tataatggca atggcaagca
     1561 gcagcattac ttcaaaatgt tgaagcagct gaccctctgg ggatatttca cctccgaggc
     1621 gggagcaacg aaagccttgc gttatatcga aacgcccggc cattatgacg gcgattaccc
     1681 ctacaagaaa ggcgataaag cctgggcgtt ataggccggg gagatgaaag ctttgaccgc
     1741 tggcatgaat ttttaggaat tcaattggaa gtttctcgct ggggtggcat ggtagcgaag
     1801 ggaaaagaga aagtttcagg ggctccggcg aacaaccgga gccttttatt catatgatca
     1861 gacctctttt atatgtagtg atcctctttg tctgcgggtc ctggatgcgc cagacggatc
     1921 cgtggacgcc ggcgcaactg atggagccgg cggatctggc ggcggcgctg aatcattcga
     1981 caaatcgtct cccccttctg gtttgtgtcg ggccctcggg gatcatcaag gggtcgatcg
     2041 agaccggccc cgcgcacgag ccgcagaatc
//
LOCUS       DATGKE010000701         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_465902, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000701 DATGKE010000000
VERSION     DATGKE010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465902"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VKU83_11900"
     CDS             <1..562
                     /locus_tag="VKU83_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI94312.1"
                     /translation="LVIHRGDWATIVAAKVNTADMAGLIRAVGRVWKSYAPQQPMRYT
                     FMDETYAKQYADVERTRNLFTSLSVLAVVIACLGLFALSAFMAERRRKEVGIRKVLGA
                     TVAQVTVLLSREFLVLVGVSIVIASPVAWWGTKWWLRDYVYRIEVGWGIFATAGLLVL
                     GIAMATIGVQAVRAARANPTGTLRSD"
     gene            complement(658..2202)
                     /locus_tag="VKU83_11905"
     CDS             complement(658..2202)
                     /locus_tag="VKU83_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLI94313.1"
                     /translation="MKLKNLAILASAVSLFAACKSHKPEQSAVTGWNYNDSKMGGFQV
                     AKETEQRTGPGLVFVQGGTFTMGATQEDVMRDWNNVPRRVTVNSFYIDQTEVANIHYR
                     EYLYWIDQVFDADEYKAVRDGALPDTLCWRSELAYNEPMVEYYFRHPSYNYYPVVGVT
                     WRQAHDFCLWRSDRVNELALIKRGYINANSIKNLQGLGQENFNTKAYLMGEYQATPDK
                     KARTKSNPLKNPNGTPRTVVTFEDGILLPNYRLPTEAEWEYAALGYIGQNPLPSKNDK
                     SKGEELIANKQVYSWANNVNGLRDTRHGSWQGTFMANFKRGSGDNMGVAGGLNDNAVY
                     TAPVKSFFPNAFGLYNMSGNVSEWVFDVYRPMSTQDDDDVAPFRGNHFQTVDYDKDGK
                     ADRDSLGRIKMRYVTDSEAHNRRNYQKGDVINYLDGDSTIAGISYGYGITTLISNKSR
                     VVKGGSWNDRPYWLSPGTRRFLEEDQDNSTIGFRCAMDRVGSPAGNGRQTGINYKTRR
                     QNTRKQ"
BASE COUNT          513 a          661 c          772 g          609 t
ORIGIN      
        1 gttggtcatc caccggggag actgggccac gatcgtcgcg gcaaaggtga atactgccga
       61 catggcggga ctgatccggg cggtgggcag ggtatggaaa agctatgcgc cgcaacagcc
      121 gatgcgctat acgtttatgg acgagaccta tgcgaagcaa tatgccgatg tggagcgaac
      181 gcggaatttg tttaccagcc tgtcggtgct ggcggtggtg atcgcgtgcc ttggactctt
      241 tgccctgtcg gcgttcatgg ccgagcggcg gcggaaagaa gtgggtatcc ggaaggtgct
      301 cggggcgacg gtcgcgcagg taacggtgtt gctcagtcgc gaattcctgg tcctggtagg
      361 cgtctcgatc gtcatcgctt cccccgttgc atggtggggg acgaagtggt ggctgcgcga
      421 ttatgtctat cgcatcgagg tcggctgggg catctttgcg acggccgggc tgctggtgct
      481 ggggattgca atggcgacga tcggcgttca ggcggtgagg gcggcgcgcg cgaatccgac
      541 cgggaccctt cgctcggact agtcaatgcg gccggcgcgt gtggacgcgg caacggctct
      601 gggcaataaa aaagccccgc caaccggcag ggctccctaa ttgtggggta agatcttcta
      661 ttgttttcgg gtattctggc gtcttgtctt gtaatttatg ccggtttgcc ggccgttgcc
      721 tgcggggctg cccacgcggt ccatggcgca gcggaaaccg atggtgctgt tgtcctggtc
      781 ttcttcgagg aagcggcggg tgccggggct aagccagtag ggccggtcgt tccagctgcc
      841 gcccttgacg acgcgggatt tgttactgat cagggtcgtg atgccgtacc cgtagctaat
      901 gccggcgatc gtggagtcac cgtcgaggta gttgatcacg tcgcccttct ggtaattccg
      961 gcggttgtgc gcttctgagt cggtcacgta ccgcatcttg atacggccga ggctgtcgcg
     1021 gtcggctttg ccgtccttgt cgtagtcgac cgtctggaag tggttaccgc ggaaaggtgc
     1081 cacgtcatca tcgtcctgcg tgctcatcgg gcgatacacg tcgaataccc attcggagac
     1141 gttgccggac atattgtaca gaccaaaagc gttcgggaag aaagatttga ccggcgccgt
     1201 atagaccgcg ttgtcgttca gaccgcccgc gacgcccata ttgtcaccgg aaccgcgctt
     1261 gaagttagcc ataaacgtac cctgccagct cccatgacgg gtgtcgcgaa ggccgttgac
     1321 attattggcc caggaataaa cctgtttgtt ggcgataagc tcttctcctt tcgacttatc
     1381 attcttcgac ggtagggggt tttggccgat ataaccgagt gcggcgtatt cccactccgc
     1441 ttctgtcgga aggcggtagt tcggaagaag gatcccatcc tcgaaggtca cgacggtacg
     1501 cggggtcccg ttgggattct tcagggggtt gctcttggtc cgggccttct tatcgggagt
     1561 ggcctggtat tcccccatca ggtaagcctt cgtgttgaaa ttctcctgac caagcccctg
     1621 gaggttcttg atcgagttgg cattgatata gccgcgcttg atgagggcaa gttcattgac
     1681 ccggtcggag cgccaaaggc agaagtcgtg agcctgtctc caggtcaccc cgacgacagg
     1741 atagtaattg taagagggat gccgaaaata atattcgacc atcggttcgt tgtaggcaag
     1801 ttcactccgc cagcaaagag tatcggggag ggccccgtcg cggacggctt tatactcatc
     1861 ggcgtcaaac acctgatcta tccagtagag gtactcgcgg tagtgtatat tggccacctc
     1921 tgtctggtca atatagaacg agttgaccgt cacgcggcgt ggaacgttgt tccaatcgcg
     1981 catcacgtcc tcctgagtcg cgcccatggt gaaggtaccg ccctggacaa agaccagacc
     2041 ggggccggtg cgctgttccg tttctttggc aacctgaaat ccgcccattt tggaatcgtt
     2101 gtaattccaa cccgtgacgg cggactgctc tggcttatgt gatttgcagg cagcaaacag
     2161 ggatacggca gatgccagga tagctaggtt tttcagtttc atgatcttga cgcttttgat
     2221 tgaacgctcg taaacaggaa aaacgaggat ttcgctcagt tattgtacaa aaatacgtgt
     2281 gttagatttc cacagttgcc cgttttcgga atgggttgca aatttaggac cgaaagccct
     2341 aaatttgccg tcccgggaca tcttcccagc atgggatttg cataccagat ttcttgtgga
     2401 ttcgtgcgca ataaaataat agtgaggccg ttcgagaata agaccggacc gtttcccgga
     2461 ccccggtgac gcaaccacaa acgctcaaaa tccgtattgc cttaaactat taacaactaa
     2521 caacttagca gcctatttcg aaaatctacc taaaa
//
LOCUS       DATGKE010000702         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_965624, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000702 DATGKE010000000
VERSION     DATGKE010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965624"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VKU83_11910"
     CDS             complement(<1..263)
                     /locus_tag="VKU83_11910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLI94314.1"
                     /translation="MTHRLTFSRSRWWRLIPIVFITYSLAYLDRANFGFGMAGGMEKD
                     LGIDAHQSSLLTSLFFLGYFFFQIPGVYAAVKSARQLIFWSLIL"
     gene            complement(324..803)
                     /locus_tag="VKU83_11915"
     CDS             complement(324..803)
                     /locus_tag="VKU83_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94315.1"
                     /translation="MRRRLLVCLLPFLAACSKTGKTPGAPSFSVSAVLNGTPTTFNAI
                     ITVDSVSTPGTVYIVAHSDSANLTPLFEITLAAGHPLKPGTYTSADSTGGNLGLIGYT
                     GWQGGSANQYTLLSDTVNLTTVNKAWFSGSFQGTCEYGVDSILTVSNGQFTVGWAQH"
     gene            958..3315
                     /locus_tag="VKU83_11920"
     CDS             958..3315
                     /locus_tag="VKU83_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HLI94316.1"
                     /translation="MNKSSLVLVALLWACGARAQQGRALTDNDYAHAETLLSYGTEPY
                     VDRGFTRPNWLSGDRFWYRVLTAKGSEFILVNPERGTRTAAFDQARLASGLSAATGQH
                     VDPDRLPFGQFSYSGDGRSIHFRSAGKNWMCDLSRYSVVPDTAKAEGFEDMNGGTRAA
                     EELSPDGKKAAYIKDYNLWVRDLTTGKETQLTTDGVKDFGYATDNAGWRHSDRPVLLW
                     SPDSKKIATFQDDQRNVGEMYLWTTNVGHPVLHQWKYPLPGDPVIPMIQRVVIEVDNP
                     KVIRLQVPPDPHRGTLSDDISSGGSFDDVDWSPDGSQLAFVSTSRDHKDEKFRIADAA
                     TGAVREVFEETVPTQFESGRSAIDWRFLPGSNEIIWYSERDNWGHLYLYDATTGKLKN
                     QITKGQWVVSRLYKVDQKKRLLYFTVAGRETENPYFNQLYKVNFDGSHLELLTPEAGN
                     HVITFSPAEDYFVDTYSKPDVPPVSVLRNLQGKIVVPLEKTDISRLEATGWKPPTPFS
                     VKAHDGVTDIYGLLFTPEKLDSGRKYPIIDYIYPGPQGGSVGSWNFLASRGDNQALAE
                     LGFVVVEIEGTSNPYRSKSFHDMSYGNMAENTLPDQVTGIRQLAGRYAYIDTGRVGIW
                     GHSGGGFATAGAMFRYPYFFKVGISESGNHDNRNYEDDWGERYDGLVANSDYAAQANE
                     AYAKNLKGKLMLAHGLMDDNVPPSNTFLVIDALEKANKDYDLVVFPNSPHGYGQFSPY
                     MMRRRWDYFVRNLAGMEPPHEYQLKMKTDPRNEGIGGFGGRGRRG"
     gene            complement(3343..4062)
                     /locus_tag="VKU83_11925"
     CDS             complement(3343..4062)
                     /locus_tag="VKU83_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HLI94317.1"
                     /translation="MFNFRHAIFPVIISLLAGCHNNNTRPAVTAGDSLSSAPAAAAAK
                     SIDSTHTVPAAEKTKTILFYGNSLTAGYGLSPDQSFPALIGHRLDSLRLPYKSINAGL
                     SGETSAGGLTRIGWVLRQPVNIFVLELGGNDGLRGIPLNSTRDNLQAIIDTVRKKYPS
                     AKIVLAGMQIPPNMGEVYSKQFHDLFPALAKKNNLTLIPFLLRDVGGIPALNQGDGIH
                     PNVAGEKIVTETVWKYLRPLL"
     gene            4091..>4754
                     /locus_tag="VKU83_11930"
     CDS             4091..>4754
                     /locus_tag="VKU83_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI94318.1"
                     /translation="MAPEIMATEAILRITNISKTYKAAGRSLHVLENVSLTVKAGETV
                     AIVGPSGSGKTTLLGLSAGLDRASSGSVELNGILLDDLDEDERAVVRNRYIGFIFQNF
                     QLMPTLTAIENVMVPMELRGEKRVRERARELMDRVGLGDRLDHYPAQLSGGEQQRVSL
                     ARAFAGQPRILFADEPTGNLDAETSERIVQLLFGLNREAGTTLVIVTHNLELAAGAQR
                     IIR"
BASE COUNT         1070 a         1191 c         1543 g          950 t
ORIGIN      
        1 aggataaggg accaaaagat caattggcgg gcgcttttta ccgccgcgta cacgccgggg
       61 atctggaaaa agaaatatcc gaggaaaaac agcgaagtca gcaatgaaga ctggtgggcg
      121 tcaataccaa gatccttctc catgccgccg gccatgccaa acccgaaatt cgcacggtca
      181 aggtatgcaa gcgaataggt aataaagacg attgggatca gtcgccacca gcgggatctg
      241 gagaaggtga gtcgatgagt caagacgtgg aagttacgta aaaaacggcg ctttgccggg
      301 ggcgtgagag accgcggcgc gtcctagtgc tgggcccagc cgacggtaaa ttgaccgttg
      361 ctgacggtta gaatggagtc gacgccgtat tcgcaagtgc cctgaaagct gcccgaaaac
      421 caggctttgt tgacggtggt gagattgacc gtgtcgctca ggagagtgta ctggttggcc
      481 gagccgcctt gccagccggt gtagccgatg aggcccaggt tgccgcccgt gctgtccgcc
      541 gaagtatacg ttcccggttt gagtggatga cctgccgcaa gcgtgatctc gaacaaaggg
      601 gttaggttgg cggagtcgct gtgcgcgacg atatacactg tgcccggcgt gctgacgctg
      661 tcaacggtga taatggcgtt gaaggtggtg ggcgtgccgt tcagcacagc ggagacgcta
      721 aaggaaggcg cgccgggtgt cttgccggtt tttgagcagg cggccagaaa gggaagcagg
      781 cagacaagga ggcgacgtct catcgggatg ggtttctctg gaaaaacgcg cggacagggc
      841 taaaaattgg cgaggtgctg caatatttta atagggacgc aggatttttt ccgcgggttg
      901 ggccggccgg taaatggatt aattagtaca ttggtcactc actttatgat catagatatg
      961 aataaatcat cattagtcct cgtcgcgcta ttgtgggcat gcggcgcccg cgcgcaacaa
     1021 ggccgggccc tgaccgataa cgactatgca catgcggaaa ctttgttgag ctatggaacg
     1081 gagccatacg tcgatcgcgg ctttacccgg ccgaactggc tgtccggtga ccgtttttgg
     1141 taccgggtgt taacggcaaa gggaagtgaa ttcatcctgg ttaaccctga gaggggtacg
     1201 aggaccgcgg ctttcgatca ggccaggctg gcctcggggt tatcggcggc aacaggccag
     1261 cacgtggacc cagaccgctt gcccttcgga cagttcagtt actccgggga cggccgctcc
     1321 atccatttcc ggtcggcagg caagaattgg atgtgtgacc tgagcaggta ttccgttgta
     1381 ccggatacgg cgaaggcgga gggttttgag gacatgaacg gcggaacgcg ggccgccgaa
     1441 gagctgtcgc ctgacggcaa gaaggcggcc tatattaaag actataatct gtgggtccgc
     1501 gacctgacga cgggtaaaga gacacagctg acaaccgacg gggttaagga cttcgggtat
     1561 gcgaccgata acgcgggctg gcgtcacagc gaccggccgg tcctgctctg gtcgccggat
     1621 tcaaagaaga ttgccacctt ccaggacgat cagcggaacg tcggcgagat gtacctgtgg
     1681 acgaccaatg tcggccaccc ggtgctgcac caatggaaat atcctttgcc gggagatccg
     1741 gttatcccga tgatccagcg ggtcgtcatc gaggtggaca acccgaaggt catccggctg
     1801 caggtgccgc ccgatccgca ccggggaacg ctgagcgacg acatctccag cgggggaagc
     1861 ttcgacgacg tggattggag cccggacggg tcccagctcg catttgtgtc gacgagcagg
     1921 gatcacaagg atgaaaagtt ccgcatcgcc gatgcggcta ccggcgcggt gcgggaagtc
     1981 tttgaggaga ccgttccaac gcaattcgag tcgggcaggt cggcgatcga ttggcgcttt
     2041 ctgccgggtt cgaacgaaat catctggtat tcggagcgtg acaattgggg acatttatat
     2101 ctctacgatg cgacgacggg aaaactgaag aaccagatca ccaagggtca atgggtcgtc
     2161 agccgcttgt acaaggttga tcagaagaaa cgcctcctgt atttcaccgt tgccgggcgc
     2221 gaaacggaga acccttattt caatcaactc tacaaggtga atttcgatgg cagccatctc
     2281 gagctattga cgccggaggc cgggaatcac gtgattactt tctcgcccgc ggaagattac
     2341 tttgtcgata cttattccaa gcccgatgtc ccgccggtga gcgtattgcg gaatttgcag
     2401 ggaaagattg tcgtgcccct ggagaagacg gatatttcgc ggctcgaggc aaccggctgg
     2461 aaaccgccga cgcccttctc ggtgaaagcg cacgacgggg ttaccgatat ctacggctta
     2521 ttgttcacgc ctgaaaagct ggattccggc cggaagtatc ccatcatcga ttatatttac
     2581 cccgggccgc aagggggcag cgtgggaagc tggaactttc tggcttcgag gggcgataac
     2641 caggcgcttg ccgagctggg tttcgttgtc gtagagatcg aaggcaccag caacccctac
     2701 cggtcgaaga gctttcacga catgagctac gggaacatgg ctgagaatac actgccagac
     2761 caggttaccg gaatccggca gctcgcgggg cggtacgctt atatagacac cggccgggta
     2821 ggcatctggg gccactccgg cggcggtttt gcgacagcag gggcgatgtt ccggtatccg
     2881 tactttttta aggtgggcat ctcggaatcc gggaaccatg acaaccgcaa ttacgaagac
     2941 gactggggcg agcggtacga cggcctggtc gccaattcgg actatgcggc gcaggccaat
     3001 gaggcgtatg cgaagaacct caagggaaaa ttgatgctgg cgcacggcct gatggatgac
     3061 aatgtcccgc cctccaacac cttcctggtc atcgatgcgt tggaaaaggc caataaagat
     3121 tatgacctgg ttgtctttcc gaacagtccg catggatacg gacaattttc tccttacatg
     3181 atgcggcgcc gatgggacta ttttgtcagg aacctggcgg ggatggagcc gccgcatgaa
     3241 taccagttga agatgaaaac ggacccacgg aatgaaggaa tcgggggatt tggtggacgg
     3301 ggaagacggg gataagtcga ttgcaggatg ccgggtggcc ggttaaagaa gtggccgcag
     3361 gtacttccag acggtctcgg tcacgatctt ttcgccagcg acattgggat gaatgccatc
     3421 gccctggttg agcgcgggga tgccgcctac atcccgcagg agaaagggaa tgagggtgag
     3481 attgtttttt ttcgcgagtg cggggaagag gtcgtggaac tgtttcgaat agacttcacc
     3541 catgttcggc gggatctgca tgccggcgag aacaatcttc gcggaagggt attttttgcg
     3601 taccgtatcg atgattgcct gcaggttgtc gcgggtgctg ttgaggggaa tgccgcggag
     3661 cccgtcgttg ccgccaagct cgagtacaaa gatattcacc ggctgccgga ggacccagcc
     3721 gatgcgcgtc agcccgccgg cgctcgtttc gccgctgagc cccgcgttga tgctcttata
     3781 gggaagacga agggagtcga ggcgatggcc gatcagggcg ggaaaggact ggtcggggct
     3841 gagcccgtag ccggcagtga ggctattgcc ataaaacaaa atcgtttttg tcttttcggc
     3901 ggcaggaacg gtatgggtgc tgtctatgga tttcgcggcc gcagccgccg gcgcgctgct
     3961 cagactatcg ccggccgtca ccgccggccg ggtgttgtta ttgtggcagc ctgccagcag
     4021 cgagatgatg accgggaata tggcgtgacg gaagttgaac ataccgctac cgattatgta
     4081 atacaaagat atggccccag aaattatggc gacagaggca atactcagga tcacaaacat
     4141 cagcaagact tacaaagccg cggggcgcag cctccacgta ctcgaaaatg tcagtcttac
     4201 agtaaaagcc ggggaaaccg tggccatcgt cgggccatcg ggaagcggca aaaccacgct
     4261 attgggatta agcgcggggc ttgaccgggc gagctccggg tcggtggagt tgaatggcat
     4321 cctgctggac gatctggacg aggacgagcg ggcggttgtc cgcaaccggt atataggatt
     4381 tattttccag aattttcaac tgatgccgac cctgacggcg attgaaaatg tgatggtgcc
     4441 catggaactt cggggcgaaa aacgggttcg ggagcgcgca agggagctga tggacagggt
     4501 aggactgggc gacaggctcg atcattatcc ggcgcagctg tcgggtggcg agcagcagcg
     4561 ggtctcgctt gccagggcgt ttgcggggca gccgaggatc ctttttgccg acgaaccgac
     4621 aggaaaccta gatgcggaga caagcgagcg gatcgtacag ttgttattcg ggctcaaccg
     4681 cgaggcgggg acgacgctcg ttattgtgac ccataacctg gaactggcgg ccggggccca
     4741 gcggatcatc cggc
//
LOCUS       DATGKE010000703         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_461922, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000703 DATGKE010000000
VERSION     DATGKE010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461922"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /locus_tag="VKU83_11935"
     CDS             <1..>2032
                     /locus_tag="VKU83_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94319.1"
                     /translation="KPLFDHKTLSPSDTQGWKAMAHNSFQYKAEHGHFPLWTEGIFSG
                     MPAYQIAMDAPGFSPQNWIYDILTLWLPNPAALFFLASICFYILALAFRVNPYVAIFT
                     GLAYAYSTYNPSILAVGHTTKMQAIAIMPAFLASLIWLYEKKYLLGTAALAVFTALLI
                     GANHPQIAYYGLITAAFMTVAYLIRWIRAKDYRHIVRVIILGGAAGLVGIACSAVVTL
                     TTIDYSKASLRNGTELATPGGSVTKTGLSDKYALSYSSYRTETFTLLVPKMYGGSDVD
                     GQIVAEDSKTMQALQDMPQQLSQQLSNFVRSYWGGIGTTAGPAYAGAIICLFALVGFF
                     LLDGKHKWWLLAAGVFTMLMSWGEYFAGFNEFLLRVLPGYSKFRAPSVIIVIPTLVLV
                     ILATLSLNRLISLSPAERTVVWKQYKKGLYLTGAVFAVLFLLYLSFDYTGESDRNLQS
                     RIASAPAQVQEYVRNFIHAIREDRQTMFLDSILRSLAYIVIAAGIGILVIKGRLKALL
                     AVILIGILSFADLITIDSQYLNADKYVDSDEAENPFQPTPADLQVMADKSYYRVFDLR
                     EGVENLTNSTATPFFHLDVTGYHPAKLGIYQDLIENQLNKYPNCQPALDMLNAKYLLL
                     PTNTGRDSAASNPGALGAAWLVRGLRYAAGPGAVMDALTNLDVKDTAILFDKD"
BASE COUNT          436 a          581 c          512 g          503 t
ORIGIN      
        1 caaaccgctt ttcgatcata agacgctttc cccgagcgat acgcagggct ggaaggccat
       61 ggcgcataat tcttttcaat acaaggcaga gcacggtcat ttcccgctct ggacggaggg
      121 cattttcagc gggatgccgg cgtatcagat cgccatggat gcgccagggt tttcgccaca
      181 aaactggatc tatgatatcc ttaccttgtg gctgccgaat cccgccgcgc tgttttttct
      241 tgcttccatt tgcttctata tcctggcgct tgcctttcgc gtaaatccct acgtcgccat
      301 ctttaccggt cttgcgtatg cctattccac ctataatccg tctatccttg ccgtcgggca
      361 tacgaccaaa atgcaggcca ttgcgatcat gccggccttt ctggcttccc tgatctggct
      421 gtatgaaaaa aagtaccttt tgggaacggc cgccctggcg gtattcaccg ctttactgat
      481 cggtgcgaat cacccgcaga tagcctacta tggcttgatt accgcggcct ttatgaccgt
      541 cgcttatctt atccggtgga tccgggcgaa agactatcgt cacatcgtcc gcgtcattat
      601 tctcggcggc gccgccggcc ttgtcggcat agcctgtagc gccgttgtaa cgcttaccac
      661 gatcgactat tcaaaagcgt ccctgcgcaa cgggacagag ctggccaccc cgggtggctc
      721 ggtaacaaaa accggcctga gcgacaaata cgcgctcagc tacagttcgt acaggaccga
      781 aaccttcacg ctgcttgtcc caaagatgta tggaggaagc gatgtcgacg gccagatcgt
      841 cgctgaggat tcaaaaacga tgcaagcgct tcaggacatg ccgcagcaat tgagccagca
      901 gctgtccaac tttgtaagaa gctactgggg aggcatcggt accacggcag gccctgccta
      961 tgccggtgcg atcatctgcc tgtttgcact cgttggattt ttcctcctgg atggcaagca
     1021 taaatggtgg ctcctcgccg cgggggtttt cacgatgttg atgagctggg gtgaatattt
     1081 tgccggcttc aacgaatttc ttctcagagt gcttccgggt tatagtaagt tcagggcccc
     1141 cagcgtgatt attgttattc cgacgctcgt cctggtgatt ctggccacac tcagtctcaa
     1201 caggctgatc agcctgtcac cggctgaaag gaccgtcgtt tggaagcaat acaaaaaagg
     1261 actttacctt accggggccg ttttcgccgt acttttcctg ctttacctca gcttcgatta
     1321 caccggagag tcagaccgga atctgcaatc ccggatcgcc tcagccccag cccaggtgca
     1381 ggaatatgtc cggaatttta tccacgccat ccgggaagac cggcaaacca tgttcctgga
     1441 tagcatcctc cgcagtcttg cctatatcgt gattgcggca ggcatcggca tccttgtgat
     1501 taaggggcgc ctcaaggcgc tccttgccgt catcctgatc ggtatcttat cttttgccga
     1561 tttgatcact atcgacagtc aatacctcaa cgcggacaaa tatgtcgaca gcgacgaagc
     1621 cgagaatccc tttcaaccta caccggccga tctgcaggtg atggccgata aatcgtatta
     1681 ccgggttttt gatctgcgcg aaggcgtgga aaacttaacg aactcgacgg caactccctt
     1741 ttttcacctt gatgtcaccg gctatcaccc ggctaaactg ggcatctatc aagacctcat
     1801 cgaaaatcag ctcaataagt atcccaattg tcagcctgca ctggacatgt tgaacgcgaa
     1861 atacctgttg ctgccgacga acaccggcag ggattctgct gcttccaatc cgggagcgct
     1921 tggcgcggct tggcttgtcc gcggtcttcg ctacgctgcc ggcccaggag cggtcatgga
     1981 cgcactgacg aatctggacg tgaaagatac cgcgatactc ttcgataagg at
//
LOCUS       DATGKE010000704         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_152536, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000704 DATGKE010000000
VERSION     DATGKE010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152536"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VKU83_11940"
     CDS             complement(<1..763)
                     /locus_tag="VKU83_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94320.1"
                     /translation="MNLRLLIGVLLSVCTACSSRKYLHFPKETGSSMGGADFYKTVAD
                     WHWKERDSLAVALILSGDVPKFLQKFVRIGASVTDSTTGKKIEAWYYVAPDYLSIGSD
                     NDWARIPLTPMAAQKIADSFNCFLPTRKMVNDIYEQAMVKLEPVPMYAFRDSSVTMWQ
                     HHLIIEGQRKGRSGLIAGIKKDVVLSGKISRDVKPDRVAIYGWHRGTGAPIQPLYTGH
                     INWYVDYSHGIRLVYRTIWVDGKAMDYTDVLKNPGL"
     gene            complement(765..1325)
                     /locus_tag="VKU83_11945"
     CDS             complement(765..1325)
                     /locus_tag="VKU83_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLI94321.1"
                     /translation="MRNLIYGINLTIDGCCDHTKVNGSEEIHEYWTQRMRDVDTIVYG
                     RKIYELMVPYWPDVAKNRSGQTAAANEFARTFDAKKIVVFSKTLEMAERKNTRIVRGN
                     LRDEIEKLKQEEGKNISLSGLDLASQVIALGLVDEYYMVVHPIVAGEGRRLMEGIGLQ
                     EKLQLRLVESSVLGSGCVALHYLKQA"
     gene            1388..1864
                     /locus_tag="VKU83_11950"
     CDS             1388..1864
                     /locus_tag="VKU83_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94322.1"
                     /translation="MKTIFVSLALLSAVKIYGQIGEIGGAVNWNVHGSAPSAGPTIAI
                     EFTPVEDWLEIEVGATRSGSNGVSEWSFDLLCKKPFAISQKFEFMVGLGPEFSSTKTN
                     GLTSTDWGAEFALDLMYWPFKKRRFGWYVEPAYDYSFGAGNEQAVGFNAGLLIAIK"
     gene            complement(1893..2360)
                     /locus_tag="VKU83_11955"
     CDS             complement(1893..2360)
                     /locus_tag="VKU83_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94323.1"
                     /translation="MQDQTITSESLLPDNFFSVAFDAAARAQVKQAAVWAKVCTLCAF
                     AGYGLELIVAIFARPDYTTETQGLRIGAFLKMGNIFGVIIASAIGVIINYFLYRFAVV
                     TARGMDSMDIGKTNEGFNNLRVYFKIYGILLIVALSLGALVLLVAIGVGLSKR"
     gene            complement(2367..2807)
                     /locus_tag="VKU83_11960"
     CDS             complement(2367..2807)
                     /locus_tag="VKU83_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94324.1"
                     /translation="MENNPEESLLQMHVDYEGGQILRETVRWSRFLSVAGIIGLALCL
                     LVVAFAGSALLAALSRVAPGIDALGGIAGAIVLTAVLIVAAIFGFLVYMLYRFSVLTR
                     RGIDHQDQAAFVEGMRCLKIYFVGSGILALLSLLSNLFSLTKLF"
     gene            complement(2823..>3214)
                     /locus_tag="VKU83_11965"
     CDS             complement(2823..>3214)
                     /locus_tag="VKU83_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI94325.1"
                     /translation="LLIVDDDGDDLQFFIDAIGEIDPRIRCLTAFNGIEALKLLETND
                     TRPDYIFLDLNMPKMNGKQCLRHLKSSPLFQSIPVIIYSTSRRPEDSTEAREMGAAAF
                     IVKPNKFYQLKNEISGVLQLTDKSLQA"
BASE COUNT          776 a          870 c          780 g          788 t
ORIGIN      
        1 gcaaacccgg gttcttaagc acatccgtat aatccatggc cttaccatct acccaaatgg
       61 tcctgtatac caatcggatg ccgtggctgt aatccacata ccaattgatg tgaccggtat
      121 ataaaggttg aatgggtgcg cccgttcctc tatgccaacc atagatggcg actctgtcgg
      181 gtttcacgtc tcttgaaatt ttccccgata gcactacatc ctttttgata ccggcgatca
      241 gaccgcttcg tccttttctt tgcccttcta tgatcaggtg gtgctgccac atagtgacgc
      301 tgctatccct gaaagcatac atcggcacag gttccagctt taccattgcc tgttcgtata
      361 tatcgttcac cattttccgg gtaggcagga aacagttgaa gctgtctgca attttttgag
      421 cggccatcgg cgttaaaggt atcctcgccc aatcattatc ggaaccgata ctaagataat
      481 ccggcgcaac ataataccag gcctcgattt ttttacccgt agtggaatcc gttacacttg
      541 cgccgatgcg aacaaatttt tgcaggaact tcggcacatc ccccgacagg atcaatgcta
      601 ccgccaacga atcgcgttcc ttccaatgcc agtcggccac cgtcttgtaa aagtcggcgc
      661 cacccattga cgaccctgtc tctttcggga aatggaggta tttacgggaa gagcatgccg
      721 tgcaaacgct taacaggaca ccgataagca gtcggagatt cattttatgc ttgtttcagg
      781 taatgaagcg caacacatcc ggatccaagg acgcttgact cgacaagccg taattgcaat
      841 ttttcctgca ggccgatgcc ttccatcaac cgtctcccct cccctgcgac gatcgggtga
      901 acaaccatat aatattcatc gaccagacca agcgctatca cctgtgaagc aagatccaaa
      961 ccgctcagtg agatattttt tccctcttcc tgcttcaatt tctctatttc gtcgcgaaga
     1021 ttcccgcgaa cgatccgcgt atttttcctt tccgccattt ccaatgtttt ggagaaaaca
     1081 acgatcttct tcgcgtcgaa tgtccgggca aattcattcg ccgctgcggt ctgcccggaa
     1141 cggttctttg ctacatcggg ccaataagga accatcaatt catagatctt ccgcccatac
     1201 acgattgtgt cgacgtcgcg catgcgttgc gtccaatatt catggatttc ttcactgccg
     1261 ttgaccttgg tgtgatcgca gcagccgtct atggtaagat tgataccgta gattaaattt
     1321 ctcatttgat tgccttagtt ttgctttccc tcaaattagt actttatttt cacggtccac
     1381 attcatcatg aaaacaatat tcgtctcgct ggcactttta tccgctgtca agatttacgg
     1441 ccaaataggt gagatcgggg gcgctgtgaa ttggaatgtt catggctctg ccccaagcgc
     1501 agggcccact atcgccatcg aatttacacc tgttgaagac tggctggaaa tagaggtcgg
     1561 agcgacccgg tccggtagca atggggtttc cgaatggagt tttgatctgt tatgcaagaa
     1621 accttttgcg atctctcaaa aattcgagtt tatggtcggc ctgggcccgg aattcagctc
     1681 cacgaaaaca aacggcctga cttcgaccga ttggggtgcc gaatttgccc tggatctcat
     1741 gtattggccc tttaagaaaa gaaggtttgg atggtatgtt gagcccgcct acgattattc
     1801 cttcggtgca gggaacgaac aagccgtcgg cttcaacgcc gggttattga tcgccatcaa
     1861 atgagcataa agacggcagg tgaacgattg ccctatctct tcgacaaccc caccccgatg
     1921 gccacgagga ggacaagcgc gccaagcgaa agcgccacga tgagaagtat gccgtagatc
     1981 ttgaagtaga cccgcagatt gttgaatccc tcattggtct tcccgatatc catactgtcc
     2041 atcccccggg ccgtcactac ggcaaaccga tacaggaaat aattgatgat gacgccgatc
     2101 gccgaggcta tgatgacgcc gaagatattg cccattttta agaacgcccc gatgcgcaga
     2161 ccctgggtct ccgtcgtata atccggccgt gcaaaaatgg ccacgatcag ctcgaggccg
     2221 taaccggcaa aggcacacag cgtgcaaact tttgcccata ctgctgcctg cttgacctgg
     2281 gccctcgcag cagcgtcgaa agcaacggaa aaaaagttat ccggcagaag ggattcggag
     2341 gtgattgttt ggtcttgcat gacggttcag aatagcttgg ttaacgaaaa aaggttgctc
     2401 agcaggctta gcagggcaag gatcccgctg ccgacgaagt agatcttcag acatcgcata
     2461 ccttcgacaa aggctgcctg gtcctgatgg tctatccccc gccgcgtcag cacggagaaa
     2521 cggtacagca tatagaccag gaagccaaag atcgccgcaa cgatcaggac ggcggttaag
     2581 acaatcgcgc ctgcgatgcc tccaagcgca tcgatgccgg gcgccacccg ggagagcgcc
     2641 gccagcagcg ccgatccggc aaacgcaacc accagcagac agagcgccag cccgatgata
     2701 ccggcgacag acaggaaacg ggaccagcgg acggtctcac ggaggatttg accgccttca
     2761 tagtccacgt gcatctggag taaggattcc tctgggttat tttccatttt ttatacgcat
     2821 tttcaagctt gcaggctttt gtccgttagc tgaaggacac ccgagatctc gttcttcagc
     2881 tgatagaatt tattcggttt tacaatgaag gcggcggcgc ccatctcccg ggcttcggtc
     2941 gaatcctccg gccgtctgct ggtagaatag ataatgacgg ggatcgattg gaaaagcgga
     3001 ctgctcttca agtgccgcag acattgcttt ccattcatct tgggcatgtt gaggtccaga
     3061 aatatataat cgggccgggt atcgtttgtc tcgagcagct tgagcgcctc gatcccgtta
     3121 aaagcggtca aacaccggat ccgggggtcg atctccccaa tggcgtcgat gaagaactgc
     3181 agatcatcgc cgtcgtcatc cacaatgagc aaag
//
LOCUS       DATGKE010000705         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_153262, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000705 DATGKE010000000
VERSION     DATGKE010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153262"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..786)
                     /locus_tag="VKU83_11970"
     CDS             complement(199..786)
                     /locus_tag="VKU83_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLI94326.1"
                     /translation="MTLFIKNMVCPRCIAAVESAIGKVGAEAIDIRLGEVKLRHPLSG
                     GQLQALGARLESLGFELLDDRRKRQIEKIKTVILQETQRLAGQKLAVSELISRSLNRE
                     YSQLSKLFSETEGITIEHFSILQRIEKVKELLIYDEMSLKQIADALGYSSSAHLSAQF
                     RKVTGMTPTAFRQRGSRLRRPLDDVGDTIKYKTNP"
     gene            920..1198
                     /locus_tag="VKU83_11975"
     CDS             920..1198
                     /locus_tag="VKU83_11975"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLI94327.1"
                     /translation="MMKALAHPARIAIVQQLIKTNACVCGGLVEELGLAQATISQHLK
                     ELKNAGLIRGTIEGTSVCYCIDAKVWAQFQKEVGALFGAYKSQGNCNC"
     gene            1246..2067
                     /locus_tag="VKU83_11980"
     CDS             1246..2067
                     /locus_tag="VKU83_11980"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HLI94328.1"
                     /translation="METSEQLKELVRKKYGEIALQEKDDNQSSCCGSGCCSTEVYNVM
                     TDDYSSLQGYTPDADLGLGCGLPTQFAAIKKGDTVIDLGCGAGNDCFVARAETGESGT
                     VIGIDFTPAMIRKARENAAKLGFSNVEFRMGDIENMPVSSGSADVVVSNCVLNLVPDK
                     VSVFKEIYRVLKPGGHFSISDIVLVGSLPAKIQAAAEMYAGCVAGASQKTAYLGFINQ
                     AGFNDILVQKEKAIVLPHDILAQYLNDGEIAAFAASGSGIFSVTVFARKPAGAVC"
     gene            2126..>2860
                     /locus_tag="VKU83_11985"
     CDS             2126..>2860
                     /locus_tag="VKU83_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLI94329.1"
                     /translation="MRIALFSDIHANLPALEALFRDLEKQKPDAVYCLGDLVGYNIWP
                     NEVVNEIRRRGIPTIAGNYDFGVGRNSDDCGCAYKTDDEKANGALSISYTNRTIGEGE
                     RAYLRTLPAHILVEFQLNPDKLILLLVHGSPRRINEYLFEDREESSLLRIIEGAGSDI
                     LCFGHTHKPFHRIIRSETDGKDRHAINIGSVGKPKDGDPRGCYVLLTLGEAGSIGVEF
                     VRFPYDVEKAAQAIERSLLPRAFADAL"
BASE COUNT          678 a          736 c          786 g          660 t
ORIGIN      
        1 tgggccgtct tctgagcaaa attgacgctg gcgtctatga cgccacgctg tttcttcaat
       61 gcagactgga cattgaccgc acaggatgcg caactcatat tcaagacggg gtatgtttcc
      121 ttcgcgatcg tctcctctgc catattatcg gtttgccggt acaaaggtat ggcctctcgc
      181 gggcgggtcg ttacagcttt atgggtttgt tttatattta attgtgtctc cgacgtcgtc
      241 caggggccgc cgcaaacggc tgcctcgttg ccggaaggcc gtcggcgtca tgcccgtaac
      301 cttgcggaac tgggcggaaa gatgagcgga actgctatag ccgagcgcgt cggcgatctg
      361 cttgaggctc atctcgtcgt agatcagtaa ctctttgacc ttttcgatcc gttgcaagat
      421 cgaaaaatgt tctatcgtga tgccttctgt ttcagaaaaa agcttgctaa gctgggaata
      481 ttcccggttc agggatcggg agataagctc cgatacggct agcttttgtc cggcgagccg
      541 ttgcgtctcc tgaaggatga cggttttgat cttttcgatt tgtcttttgc gcctgtcgtc
      601 aagcagctcg aatccgaggg attcgagtcg ggcgcccagc gcttgcaact ggccgccgct
      661 aaggggatgg cgaagtttca cctctcccag ccggatgtca atagcctcgg ccccgacctt
      721 tccgattgcg gattccacgg ctgcgataca gcggggacaa accatgttct ttataaaaag
      781 tgtcaaccgg aaattttaag caaaactaaa aattaatttt gattataaat agttaatcgt
      841 aatattgcaa ttaaacaatg aattagtggg agtaaccagg tcagaaatat tcaccgaaaa
      901 gcaaaaccgg ctcgccggca tgatgaaggc tctcgcccac cccgcccgga tcgccatcgt
      961 tcagcaatta atcaaaacga acgcctgtgt ttgcggcggc ctggttgaag agctggggct
     1021 tgcgcaggca accatctcgc agcacctcaa ggaattgaag aatgccgggc tcatccgtgg
     1081 cacgatagag ggcaccagcg tgtgctattg catcgacgcc aaggtctggg cgcaatttca
     1141 aaaagaggtt ggggccttgt ttggggcata taagagccag ggcaactgta actgctgaat
     1201 ccggcggttt accggagtca atgtgattaa acgtcaatat aatatatgga aacaagtgaa
     1261 caactcaagg aactggttcg caaaaaatat ggtgaaattg ccctccagga gaaagacgac
     1321 aaccaatctt cgtgttgtgg atcgggctgc tgctccaccg aagtttacaa tgtaatgacc
     1381 gacgattaca gttcgcttca agggtacact cccgatgcgg acctcggcct cggctgcggc
     1441 cttcccacgc agtttgcggc gatcaaaaaa ggtgatacgg tcattgacct cggatgcgga
     1501 gcaggtaacg attgttttgt cgcccgcgca gaaacggggg aatccggaac cgtgatcggg
     1561 atagatttca ctccagccat gatccggaag gcgcgggaaa atgccgcaaa gcttggcttc
     1621 agcaatgtcg aattccggat gggagacatc gaaaacatgc ccgtcagcag cgggagcgcc
     1681 gatgtcgtcg tttcgaactg tgtgctgaat cttgtcccgg acaaagtctc cgtctttaag
     1741 gagatttatc gcgtcctgaa acccggcggc catttcagca tttccgacat cgtactcgtc
     1801 ggatcattgc cggcgaaaat tcaggccgcg gctgaaatgt atgcgggttg cgtggcgggc
     1861 gccagtcaga agaccgcata cctgggtttc attaaccagg cgggtttcaa cgacattctg
     1921 gttcagaaag aaaaggctat cgtccttcca cacgatatcc tggcgcagta cctgaatgac
     1981 ggggagatcg cggccttcgc ggcatcaggc tccggaatct tcagcgtaac cgtatttgca
     2041 agaaaaccgg caggcgccgt ttgctgaccg cccgggtgct cggcgcagca ttagtaccct
     2101 tctggaagtt tttaaacgtg aagaaatgag aatagcgctg ttcagcgaca tccatgcgaa
     2161 cctgccggcc ctcgaggctt tattcaggga tttggaaaaa caaaaaccgg atgccgttta
     2221 ctgcctcggt gaccttgtcg gctacaatat ttggccgaat gaggtcgtga atgagatccg
     2281 gcgccgcggc atcccgacga tcgccggaaa ctatgatttc ggtgttggtc gcaacagcga
     2341 cgactgcggc tgcgcctaca agaccgatga cgaaaaggca aacggagcgc tgtcgatctc
     2401 ctataccaac cggaccatcg gcgaaggcga acgcgcctac ctccgaacgc ttcctgcgca
     2461 tatccttgta gaatttcagc tcaacccgga caagctcatt ctcttgctgg tccatggcag
     2521 tccacgccgc atcaacgaat atttgtttga agatcgtgag gaaagcagcc tcttacggat
     2581 catcgaggga gccggctcgg atatcctttg ttttgggcac acgcacaaac ctttccaccg
     2641 gataatccgg tcagagaccg atggaaagga ccgccacgcc attaatatcg gttcggtcgg
     2701 caagccgaag gatggcgatc cgcgaggttg ttatgtcttg ctgaccctcg gcgaagcggg
     2761 aagcataggg gtagagtttg tccggttccc gtacgacgtc gaaaaagccg ctcaagccat
     2821 tgaaagaagt ttgttgcccc gggcattcgc cgatgccctg
//
LOCUS       DATGKE010000706         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_775275, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000706 DATGKE010000000
VERSION     DATGKE010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775275"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VKU83_11990"
     CDS             complement(<1..1562)
                     /locus_tag="VKU83_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94330.1"
                     /translation="MSKLYFANAVRHLRRSPLFTTLNIVGLAISISACWIIFRLVNFE
                     FSYDAHIPDRQHVYRVVSGFRFDEKPSYNGGVSAPLYQALRKEATGLKRVVPVMTKSV
                     KAVQTNPSNGRPVVVEDQNGIAETDGSYFGLVPYHWLAGDPGRALQAPNEVVLTESRA
                     RQYFPDKMPQDVLNRTLTYYGYRDTVTRTIKGVVADLAGPTEFTSKEFCSLPVNEYTL
                     ASWTNTNFSDRLYLQLDDKAVPANVLSNIERLVAQKTRQFEESQKDNFTFKRWFQLLP
                     LSESHFSTYIEEYGHKANKRILYGLMGIAFFLLVLACINYINIGVASIPNRSKEIGVR
                     KTLGSGRSALIGQFLTETFLVSLMACALAYILGWGGFRLLKDILPPGLEPWGGIGQVT
                     GFMVLLAILVTLLSGLYPGWLITKVKTVHVFRRVFVLQHRGGRIGFQKALIVFQFVIA
                     LVFISSAMIVGSQLHYVLDSDMGFDKDAVVLASVPWKLTSNPKYQDRQFSLLAELRTL
                     PGILDVALGDEPL"
     gene            complement(1697..2008)
                     /locus_tag="VKU83_11995"
     CDS             complement(1697..2008)
                     /locus_tag="VKU83_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLI94331.1"
                     /translation="MMSNILNRFFTPSPDFKDLRKKGAIILDVRTLQEFSSGHIDGAV
                     HIPLDLIGRNLAELKQKNLPVIACCASGMRSGAAKRILTAAGIEAYNGGPWQTLDHKL
                     K"
     gene            2122..2772
                     /locus_tag="VKU83_12000"
     CDS             2122..2772
                     /locus_tag="VKU83_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLI94332.1"
                     /translation="MIEEQRIRQEFPSFETALVVEMAKVGDIKHFREGESLVKTGQQF
                     RSTLLVLEGLVKIYREEEEGNEFFMYYLQPGQACALSMIAASGREISQIKASAMTETE
                     AISIPLEAMDRWVKAFTSWYHFVLESYRARFDELLSTIDHIAFRNMDERLLFYLRKQR
                     ETLKTARLTIAATEIAADLNSSREVISRLLKKLADRGDIRMERNVIELLRLDNHGA"
     gene            complement(2794..3258)
                     /locus_tag="VKU83_12005"
     CDS             complement(2794..3258)
                     /locus_tag="VKU83_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLI94333.1"
                     /translation="MNHEISAHWMGKMQFNALVNGHTVVMDAPERVGGEDNGPIPKPL
                     ILTALSGCTGMDIVSLLRKNQCEVTNFEIDVRGELSKAHPLQYVAIHLDYLFQGPPEY
                     QDAALQAVTLSQEKYCGVSDMLKKIMPVTWTVVYNGNTIFSNQEGIVEASRN"
     gene            complement(3322..>3652)
                     /locus_tag="VKU83_12010"
     CDS             complement(3322..>3652)
                     /locus_tag="VKU83_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLI94334.1"
                     /translation="GGRWLYAMVSPDNVPSGWSLVEFLKIHPKSSFSTRNTFADENGN
                     QKSAAFSLVRNSFEEGAETTTVYIEKKFDDLAALEMIVTMGFKEGMTAAMNNLDEYFR
                     SRVGART"
BASE COUNT          827 a          915 c         1042 g          868 t
ORIGIN      
        1 agcggctcat cgccaagggc gacgtccaga atacccggga gggttcgcaa ttccgccagc
       61 aggctgaact gcctgtcctg gtatttgggg ttggaggtga gtttccaggg gacgctcgca
      121 agtacgacgg catccttgtc aaatcccatg tccgagtcga gcacataatg aagttggctg
      181 cccacgatca tcgcgctact gatgaacacg agggcgatca caaactggaa cacgatgagg
      241 gctttctgga atccgatccg gccgccgcgg tgctgcagga caaagacccg ccggaagacg
      301 tggacggtct tgactttggt gatcagccag cctgggtaga gaccggatag caacgtcacc
      361 aggatggcca gcagcaccat aaagccggtc acctgcccga tcccgcccca aggctccagg
      421 cccggaggaa gaatatcctt gagcaggcga aagccgcccc aaccgagaat ataggccagc
      481 gcacaggcca tcagagacac caggaacgtt tctgtaagga actgtccgat cagggcggac
      541 cgcccgctgc caagcgtctt gcgcacgcca atttcctttg aacgattggg aatcgaggcg
      601 acgccgatgt tgatataatt gatgcacgcc agtacgagca aaaagaaggc aatccccatc
      661 aatccgtaca atatcctttt gttggcttta tggccatatt cttctatata agtggagaag
      721 tgagattcgc ttagcggcaa taactgaaac catcttttga acgtaaaatt gtccttttgc
      781 gattcctcaa attggcgggt tttctgtgct accagccgct cgatgttcga aagcacgttg
      841 gcgggaacgg ctttgtcgtc cagttgcagg taaagccggt cactaaaatt ggtattggtc
      901 caactggcga gtgtatattc gttcaccggc aaggagcaga attctttcga cgtaaattcg
      961 gtcgggcccg ccaggtcggc gaccacccct ttaatggtgc gcgtaaccgt atcgcgatag
     1021 ccatagtagg tcagcgtgcg gttgaggacg tcttgcggca ttttatccgg aaaatactgt
     1081 ctggcccggc tttcggtgag caccacctcg ttcggggcct gcaaagctct accgggatct
     1141 cctgccagcc aatggtaagg caccaggcca aaataggagc cgtcggtctc ggcgatccca
     1201 ttctggtcct cgacgacaac gggccttccg ttggaaggat tggtttgtac ggccttgact
     1261 gattttgtca tgaccgggac gacgcgcttg aggccggtgg cttccttacg caatgcctgg
     1321 tagaggggcg cagaaacacc gccattatag gacggctttt catcgaacct gaatccggag
     1381 acgacgcgat aaacatgttg tctgtcaggg atatgcgcat cgtagctgaa ttcaaaattc
     1441 accagccgga aaatgatcca gcacgcgctg atgctgatgg ccagcccgac aatgttgagc
     1501 gtggtaaaaa ggggcgagcg ccggagatgc cgtacggcgt tcgcgaaata cagtttggac
     1561 atagctatcc tggttgtggc atcaagttct gaaatttatt gccggcacag gcatcttacc
     1621 gccgatttat tttttccggt ccgtgacggg cgatggtaga ttcttcggcc ggtaaaagcg
     1681 gcgccggaga aggaacttat ttcagtttgt ggtccagcgt ctgccagggc ccgccgttgt
     1741 atgcttcgat tccggcggca gtaaggattc gtttggcggc gccgctgcgc atcccgctgg
     1801 cgcagcaggc gatcacgggc aggttctttt gtttcagttc ggccagattt cgtccaatga
     1861 ggtctaaagg aatgtggacg gcgccgtcta tatgtccgct gctgaattcc tggagtgtac
     1921 gcacatccag gatgatcgca cctttctttc ggaggtcttt gaaatcgggg gacggggtga
     1981 aaaatcgatt tagaatgttt gacatcatat ctctatggtt gtttgtacaa aaatatctta
     2041 gagcaagagc taatcgcgtg acaaaagtca cgggagacgg ttattttcac cgcgcgtcgt
     2101 aatattgttc aaataatcac catgatagag gagcaacgga tccgtcagga atttccttct
     2161 ttcgaaacag cgctggttgt cgagatggca aaggtgggcg atataaaaca ttttcgggaa
     2221 ggagagtccc tcgtgaaaac cggccagcaa ttccggtcga ccctgctggt gctggagggg
     2281 ctggtcaaaa tctaccggga ggaggaggag ggtaacgaat tttttatgta ttacctgcag
     2341 cccggacagg cctgtgcgtt atccatgatc gctgcctccg gccgggagat cagccagatc
     2401 aaggctagtg ccatgacgga gactgaagcc atatctatcc ccctggaggc gatggaccgg
     2461 tgggtgaaag cgtttaccag ttggtatcat ttcgtattgg agagttatcg ggcgcgcttt
     2521 gacgagctgt tgtcgacgat tgaccatatt gcctttcgca acatggacga acggctgttg
     2581 ttctatttgc gcaagcagcg cgagacattg aagacggccc gccttacgat cgctgccacc
     2641 gagatagcgg cggatctgaa ttcatcgcgg gaggtgattt cccgcctgtt aaagaaactg
     2701 gccgaccgtg gggatatccg gatggaacgg aacgtgatcg agctgttgcg actggataac
     2761 cacggcgcct gaagtttgac agggtgcggg ttgctaattc cttgacgctt ccacgattcc
     2821 ctcctgattg gagaaaatgg tattaccgtt atagacgacg gtccatgtaa cgggcatgat
     2881 cttcttcagc atatcgctga cgccgcagta tttttcctgg gacagcgtaa cggcctgaag
     2941 ggcggcatcc tggtattccg ggggcccctg aaaaaggtag tcgagatgaa tggccacata
     3001 ttgcaagggg tgcgctttgg agagctctcc ccgtacgtcg atttcgaagt tggtgacttc
     3061 gcactggttc ttgcgcagca gggatacgat atccatgccc gtgcacccgc taagcgccgt
     3121 caggatgagc ggttttggaa tagggccgtt gtcctcgccg ccgacccttt ccggcgcatc
     3181 catgacgacg gtatggccgt taacgagtgc gttgaattgc atcttgccca tccagtgggc
     3241 ggatatttcg tgattcataa tggatactcc ttattttcat ccaaaggtcg atggatattt
     3301 cgacaggaac tgtgacttat gtcacgtcct tgcgccgacc cgggagcgga aatattcatc
     3361 caggttgttc attgccgcgg tcatgccttc cttaaagccc atcgtgacga tcatttcaag
     3421 ggcggcaagg tcatcgaatt ttttttctat gtagaccgtc gttgtctccg ctccttcttc
     3481 gaaggaattc cggaccaggg aaaacgcagc cgatttttgg ttcccgtttt cgtctgcaaa
     3541 ggtattcctg gtggaaaaac tggacttggg gtggatcttg agaaattcaa ccaatgacca
     3601 acccgaaggg acgttgtcgg gcgaaaccat ggcatataac caacggccgc ct
//
LOCUS       DATGKE010000707         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_281980, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000707 DATGKE010000000
VERSION     DATGKE010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281980"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="ilvC"
                     /locus_tag="VKU83_12015"
     CDS             <1..773
                     /gene="ilvC"
                     /locus_tag="VKU83_12015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HLI94335.1"
                     /translation="LWPTVKKHLTAGKALYFSHGFGITFNEQTGIIPPKDVDVFLVAP
                     KGSGTSLRRMFLQGRGLNSSYAIFQDATGKARDRVIALGIAVGSGYLFETDFRKEVFS
                     DLTGERGTLMGAIQGIFAAQYEVLRKKGHTPSEAFNETVEELTQSLMPLVAENGMDWM
                     YANCSTTAQRGALDWWKKFRDATLPVFTELYESVAAGKESQRSIDSNSQPDYREKLDA
                     ELKELRESELWQAGKTVRSLRPENQPVGAKRPEAGSYA"
     gene            811..2115
                     /gene="ilvA"
                     /locus_tag="VKU83_12020"
     CDS             811..2115
                     /gene="ilvA"
                     /locus_tag="VKU83_12020"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF006390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HLI94336.1"
                     /translation="MDQSVNNTGLNALSAPGIEPGGDMRKLDFLAAARRLAKVVHRTP
                     LAFNQRLSRKFGCRVFLKREDLQVVRSYKLRGAYNMMSTLPAEQLQKGVVCASAGNHA
                     QGFAYSCHKLNVKGVVFMPIITPRQKINQTRMFGEDNIEIVLTGDTFDDCNTAAIAYT
                     RERGMTFIPPFEDLRIIEGQGTVAVEILEDLPDIDYLFVPVGGGGLASGAGSYFKIHS
                     PGTRVIGLEPEGAPSMQAAFQAGRPVSLDSIDRFVDGAAVKRVGDLTYRICSEVLDDI
                     HLVPEGKVCSTILKLYNEDAIVVEPAGALSIAALDDYAADIKGKTVVCVVSGSNNDID
                     RMQEIKERSLQYEGLKHYFLIRFAQRPGALKEFVNHVLGPDDDITRFEYMQKHNKETG
                     PALVGIELSCREDYQKLLENLSTHNITYTELNKNDTLFGYLV"
     gene            2197..2409
                     /locus_tag="VKU83_12025"
     CDS             2197..2409
                     /locus_tag="VKU83_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94337.1"
                     /translation="MVENGLYSPEFERDACGIGFVVNIKSNKSHQIVSDALTILENME
                     HRGACGCENNTGDGAADSDQDEPDLR"
BASE COUNT          578 a          690 c          672 g          478 t
ORIGIN      
        1 agttatggcc taccgtcaag aaacatctta ccgccggcaa agccctttac ttttcgcacg
       61 gcttcggcat cactttcaac gaacaaaccg gcatcattcc gccaaaggat gtggacgtct
      121 tcctcgtcgc cccgaaaggc tccggcacct cgctccgcag aatgttcctg cagggacgcg
      181 gcctcaacag ctcctacgcc attttccagg acgctaccgg caaggcccgc gatcgcgtca
      241 tcgcgctcgg catcgccgtc ggaagcggtt atctttttga gacggatttc cgcaaggaag
      301 tgtttagtga tctcaccggc gagcgcggca ccctgatggg cgccatccag gggatctttg
      361 ctgcgcaata tgaagtgctg cgcaagaaag gacacacccc ctccgaggcc ttcaacgaga
      421 cggtcgaaga gctgacacaa tcgctcatgc cccttgtcgc tgaaaacgga atggattgga
      481 tgtatgccaa ttgttccacc acggcgcagc gtggcgccct cgactggtgg aagaaattcc
      541 gggatgcaac gcttcctgtt tttaccgagc tgtacgaaag cgttgccgcc ggcaaggagt
      601 cgcaacgttc catcgactcg aacagccagc cggactaccg cgaaaaattg gacgcggagc
      661 tgaaagagct gcgcgaaagc gaactctggc aggcgggtaa gacggtgcgc agcctccggc
      721 cggaaaacca accggtaggc gcgaaacggc cggaggcggg gtcgtatgcc taaccgtacg
      781 gaataagatt caaacaaaaa tcaacaacca gtggaccagt ctgtcaataa taccggcctt
      841 aacgcccttt cggcccccgg catcgagccg gggggcgaca tgcggaagct ggacttcctt
      901 gccgctgcca ggcgtctcgc caaagtggtg caccggacgc ctcttgcatt caaccagcgg
      961 ctttcgcgca agttcggctg ccgggtattc ctgaaacgcg aagaccttca ggtcgtgcgt
     1021 tcatacaagc tccgcggcgc gtacaatatg atgagcaccc ttcctgcgga gcagctgcag
     1081 aaaggcgttg tctgtgcgag cgcaggcaac catgcgcagg ggttcgctta ttcctgccac
     1141 aagctgaacg tgaaaggcgt cgtgttcatg ccgatcatta caccgcgcca gaagatcaat
     1201 cagacccgga tgttcgggga agacaatatc gagatcgtcc tgaccggcga cactttcgat
     1261 gactgcaaca cggcagcgat cgcctataca cgcgaacgtg gcatgacctt tattccgccc
     1321 tttgaagacc tccggataat cgaaggacag ggaacggtgg ccgtggagat cctggaggac
     1381 cttccggaca tcgactatct ttttgttccc gtcgggggcg gcggcctggc gtccggcgcc
     1441 ggctcctatt ttaagatcca ttcacccggg acacgcgtca tcgggctcga accggaaggg
     1501 gcgccctcga tgcaggccgc ctttcaggcg ggacgtccgg tctccctcga ttccatcgac
     1561 cggttcgtcg acggcgcagc ggtcaaacgc gtgggcgatc tgacctaccg gatttgcagt
     1621 gaggtgctcg acgacatcca cctcgtgccc gaaggcaaag tatgttccac catcctgaag
     1681 ctgtacaacg aggacgccat cgtcgtcgag ccggccgggg ccctcagcat cgcggcgctg
     1741 gatgactacg ccgccgatat caagggcaaa accgtcgtct gcgttgtcag cggaagcaat
     1801 aacgacatcg accggatgca ggagatcaaa gaacgatcgc tgcaatacga ggggcttaaa
     1861 cactatttcc tcatccgctt cgcgcaacgc ccgggcgccc tgaaggagtt tgtcaaccat
     1921 gtcctcggac cggatgacga catcacgagg ttcgaataca tgcaaaagca caataaggag
     1981 accgggcccg cgctggtcgg catcgaactg tcctgccggg aggattacca aaagcttcta
     2041 gaaaatttat ccacacataa tatcacttat acagaactga ataaaaatga cacattattc
     2101 ggctatttag tgtaatttta gagagaaccg gaggcttgcc atacgaaatt tcaaatttta
     2161 cggtaaataa ttgaataata tctaatactg ctgaggatgg ttgaaaatgg tctatactct
     2221 cctgagtttg aacgtgatgc gtgtggcatt gggttcgtag tcaatatcaa gagcaacaag
     2281 tcccaccaga tcgtctcgga tgcgttgacg atcctggaga acatggaaca tcgcggagcc
     2341 tgcggttgcg agaacaatac cggcgacggc gcagccgaca gcgatcagga tgaaccagat
     2401 cttcgatagg aacatgcg
//
LOCUS       DATGKE010000708         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_882824, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000708 DATGKE010000000
VERSION     DATGKE010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882824"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VKU83_12030"
     CDS             <1..1718
                     /locus_tag="VKU83_12030"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HLI94338.1"
                     /translation="EIFRLMPTEWKKMLLHGMMGADLIGFHTYDYVQHFLQSVKMILG
                     VENYFHNLQYQDRLVRIDLFPIGIDYGKFHLAATDPEVQEIRDKARSNLEDKKIIFSV
                     DRLDYTKGLMQRLSGFEYFLERYPEWREKVVFILNIVPSRSTIPAYSDRKRQIEEKIG
                     TINGKFSTIGWQPVLYRYTHMSFNELVALYQAADAALITPLRDGMNLVAKEYVASSTG
                     KGVLILSELAGAASELNEAILVNPTDVFDVASSITRALAMPLYEQRNRISLMQRRLQE
                     YDVFKWVNDFMDQLAQVKQEQQKQKVRVLDDKALAGIYRHYYGAGNRCLLLDYDGTLV
                     PFTRIPSEAEPDNTVRELLTRLASDHKNHVVIISGRDGSSLDRWLGELPITLVAEHGA
                     SIKMRNGAWQQSVSVSDLWKEEIRRIMQLFVLRCAGSFVEEKPNTIAWHYRNTQTGLG
                     FSRSRELLNTLSQLIQNTTLQVIDGNKVVEVRVAGFDKGSTALKIISEQDPDFVLCMG
                     DDTTDEDMFKALEGEAYTIKINGGASAAQYTILSQQRVAALLNALLQPPAQSGVGRAV
                     SEANR"
     gene            1737..>2198
                     /locus_tag="VKU83_12035"
     CDS             1737..>2198
                     /locus_tag="VKU83_12035"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HLI94339.1"
                     /translation="MAVHKYNMGVIGNCTYLAYIDLRADVKWMCMPRFDSSFLFGSLL
                     DESNGGHFFIRPAADECEVRQYYVPNSNVLCTEFTCRDGKFVVKDCAPRIRIYERQFR
                     PLMLVRKVELLEGEPAVTVTCQPRGDYGKVIPDAVIGSNHIRYLNLDQQVRL"
BASE COUNT          522 a          579 c          634 g          463 t
ORIGIN      
        1 atgagatatt ccggctcatg ccgaccgaat ggaagaagat gttgttgcat gggatgatgg
       61 gcgccgactt gatcggattc catacctatg attacgtgca gcatttcctt cagtcggtga
      121 agatgatcct cggcgtcgag aattattttc acaatctgca atatcaggac agactggtac
      181 gcatcgacct cttcccgatc ggcatcgatt acgggaaatt tcatctggca gccacggatc
      241 ccgaagtgca ggagatccgc gacaaggcga ggagcaacct ggaagacaag aagatcattt
      301 tttctgtgga ccggctcgac tataccaagg gcctgatgca gcgcctgagc ggcttcgaat
      361 atttcctgga acgatacccg gagtggcggg aaaaagttgt ctttatcctg aatatcgttc
      421 cttcaaggag cactatcccc gcctacagcg atagaaagcg acagatcgag gaaaagatcg
      481 ggacgatcaa cggcaaattt tcgaccatcg gctggcagcc ggtcctttac cggtacaccc
      541 atatgagctt caatgagctg gtggccctat accaggcggc cgatgccgcg ctcatcacgc
      601 ccctgcggga cggaatgaat ctggtagcca aggaatatgt ggcgagcagc accggaaagg
      661 gtgttctgat cctcagcgag ctggcgggtg cggccagcga attaaacgag gctatcctcg
      721 taaaccctac ggatgtcttc gacgtcgcat cctccattac ccgggcattg gccatgccgc
      781 tctacgaaca gcggaaccgg atctcgctga tgcagcggcg attgcaggaa tacgacgtct
      841 ttaaatgggt taatgatttc atggatcaat tggcgcaggt gaaacaggag caacaaaaac
      901 aaaaggtgcg tgtcctcgac gacaaggccc tggccggcat ctaccggcat tattatggcg
      961 cgggtaaccg gtgcctcctg ctggactacg acggaacgct ggtgcctttt acgcggattc
     1021 catcggaagc ggagccggac aacacggtgc gtgaactgct cacgcgactc gcgtcggacc
     1081 acaagaacca tgtggtaatc atcagtggcc gggatggcag ctcgctggac cgctggctgg
     1141 gggaacttcc catcaccctg gtcgcggagc atggtgcgag cataaagatg cgaaatggcg
     1201 cctggcaaca gtcggtttcg gtttccgacc tatggaagga agagatccgc aggatcatgc
     1261 aactcttcgt attacggtgc gccggatcct ttgtggagga aaagccgaat accattgcct
     1321 ggcattaccg caatacccag acggggcttg gattttcgcg gtccagggaa ctgcttaaca
     1381 cgctttcgca actgatccag aatacgactc tccaggtcat agacggaaat aaggttgtgg
     1441 aggtgagggt cgccggattc gataaaggat cgacggccct gaagatcatc agcgagcagg
     1501 atcccgactt cgtcctctgc atgggcgacg acactaccga cgaagatatg ttcaaggcat
     1561 tggagggaga agcgtatacc atcaagatta acgggggcgc atccgccgcc caatatacga
     1621 tcctgtcgca gcaacgggtc gcggcgttac tcaatgcact cctccagcct cctgctcaaa
     1681 gcggcgttgg ccgcgcggtt tcggaggcga atcgataacc ggttaatccc caaaagatgg
     1741 cggttcacaa atacaatatg ggtgtcatcg gcaactgcac ctatctggct tatatcgacc
     1801 tgcgcgcaga cgtcaaatgg atgtgcatgc cgcggttcga cagcagcttc cttttcggtt
     1861 ccctcctcga cgaatcgaat ggcggacatt tcttcatccg gccggccgct gacgagtgcg
     1921 aggtgcggca atattatgtg cccaacagca atgtcctttg tactgagttc acgtgtcgcg
     1981 atggcaaatt tgtcgtcaag gactgtgcgc cgcggatcag gatctatgaa cggcagttcc
     2041 ggcctttgat gctggtacga aaggtggagc tgctggaagg cgaacctgcc gtgacagtga
     2101 cctgccagcc aagaggcgat tacgggaagg tcattccgga cgcggtcatc ggcagcaatc
     2161 atatccggta tctgaacctg gaccagcagg tgcggctg
//
LOCUS       DATGKE010000709         7158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_672284, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000709 DATGKE010000000
VERSION     DATGKE010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672284"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VKU83_12040"
     CDS             <1..919
                     /locus_tag="VKU83_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94340.1"
                     /translation="SGPGMALKSEAMGLAVMLEIPLVICDIQRGGPSTGLPTKTEQSD
                     LLQAYYGRNGECPMPVVAASTPSDCFEAVYEAVRIAVHHMTPVIFLSDGYIANGAEPW
                     KFPKSADLPPIEVQFKTQLGAEEAVFQPYLRDENLVRPWAIPGTAGLEHRIGGLEKQN
                     VTGNVSYDPDNHQLMVKIRDEKVARIADHIPPQRLDSGPETGDLLVLGWGSTYGAIKS
                     AVATLQKEGLAVSHAHLRYLRPFPSNLGEILHKFRQVLIPEINNGQLIRIIRDQFLID
                     AAGYHKIMGVPITRTELLVKMREMLGANN"
     gene            complement(916..3165)
                     /locus_tag="VKU83_12045"
     CDS             complement(916..3165)
                     /locus_tag="VKU83_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HLI94341.1"
                     /translation="MDYIREYRKFIHSHYLNAAIRITVGVSLPCALLALVHHLSAGIV
                     VSIGAMCVGNTDNPGPIHHRRNGMAVGILVVFVVALVTGWASASPFLTGCCVLLFCFV
                     FSLMGIYGSRATSIGLNALLIMVLNIGHHVQGWDVVRNALYVLAGGLWYMGLSLLLYS
                     FRPYRLIQQALGDCVQATAGYLRIKASFYSPQVDYDKSYRQLLDSQSSIQVKQDLLRE
                     LIFKSRNIVRESTLTGRILTMIFLDSIDLFERIMTSQQDYRLLHRLFGDSQLLADCRK
                     LILDLASELDEIGLAIMAGRPSVDRSTLSIRIRELRAKLALNPPEGVADLGNILDSIE
                     DINERLHVLQAFTTYDRQLIRHFPADFDYGRFAARQNMDRKLITDNLTLESNIFRHSL
                     RVSVATVTGYIVSRFLPVGHGYWILLTVIVILKPVYSLTKQRNFQRLTGTLCGAVLGF
                     LLIYFIHDRPALFAVMIFFMIGTYAFLRTNYLVAVIFTTPYVLLLFHLLYPVGFKVII
                     ADRVLDTLIGSAIAFLANIFIVPTWEHQRIGDTMAAALKANISYFRDVAAAYFGRPVG
                     VQDYKLSRKHAFVALANLSDAFGRVLSEPRRRQRRMTEMHQFVVASQMLTSYVATLAY
                     YEKPFAATFPGKAWQPVADGIVGVLESAVAVLQEGVDGADHAAGGARQALPAPQRPQV
                     LQLPEAPKLPASGKDRPAAAKAVEDQFGFIAKVSADLYRLSVILHPVEQTAEAAERRM
                     KGTGDPRAV"
     gene            3267..4712
                     /locus_tag="VKU83_12050"
     CDS             3267..4712
                     /locus_tag="VKU83_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HLI94342.1"
                     /translation="MQAIIEEIRRLFRTYSPYPITGLDVIPQSGSNRIYFRVRTAEDT
                     TIATYNDNIRENGTFIYFSRHFRECGCPVPEIYFVNEACTIYFQEDFGDHSLLSELEK
                     SGHNDSAYAWFRESLQQLARLQIRGNEGLDYSKTITSREFGKQAILSDLLYFKYYFLD
                     TLGIPYDKELLIDDFERLSGYLAQAEHRYFLFRDFQSRNILLKDGQVHFIDFQGGMKG
                     ALQYDVASLLWQAKAELSDPWKDSLLEHYMGCVEDLLGEKLDRPQFVDRYNGYVLIRL
                     LQVLGAYGFRGLFERKAHFLISIPLGLRNLQWFVRNRHPGIALPEFDRILQLVVRDEV
                     IHRFEPVQADQDSPLVIHIRSFSYRKSIPADESGNGGGFVFDCRGILNPGRILEFKSL
                     TGRDKAVKDYLEQQTRMPEFLNSAYGIVDISVEDYIQRGFSSLCISFGCTGGQHRSVY
                     AADALARHLRNKYHVKVDLQHIEQEAKNWEN"
     gene            4712..5608
                     /locus_tag="VKU83_12055"
     CDS             4712..5608
                     /locus_tag="VKU83_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94343.1"
                     /translation="MYYLVYGLLYGISLLPTWILYGVSDAMALTLYAVARYRRKIVLD
                     NLRLAFPEKTEAERLRIAKEFYRNFTDSFVETIKLISAGPAWLEKHFVIDNPEVMEDF
                     AREGRKIQLFLGHLFGWEVANVAMPRLTRFKFIVVYMPVTNKTFERLFLRLRGRTGTV
                     LLPATQLQRAILPYRNTVYALALVADQAPGGPENSYWLNFFGRPTAFIRGAERGARTG
                     DIPAVFARVYKTGRGLYRSRLTLMTERPAALPEGELTRRYRDLMETAIREQPATWLWS
                     HKRWKFEWNETLAPFWIDGQKI"
     gene            5766..6116
                     /locus_tag="VKU83_12060"
     CDS             5766..6116
                     /locus_tag="VKU83_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLI94344.1"
                     /translation="MKNSKPGVALIEDRVLLHCRPVLVLKDRELEFMRLVCTECTYKQ
                     IADRMYLSPRTIDGYRDALFEKLNVRTRVGLAMFAVRTGIVHVASLPASDVWDEKQAT
                     PDRRERRAARLAGR"
     gene            complement(6123..>7158)
                     /locus_tag="VKU83_12065"
     CDS             complement(6123..>7158)
                     /locus_tag="VKU83_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLI94345.1"
                     /translation="AEPLLFTGDTLFVGDVGRPDLSSGNLSREELASMLYDSLQTRIL
                     PLPENTVIYPAHGPGSSCGKNLGPGTQSTLRDEKETNYALRAASKEEFVRAVTDGLDA
                     PPSYFSINARINREGYGSLEEVLANGLKPLDLETFKRGMGEEGAILLDTRPAATFVAG
                     FIPGSISIGLDGRFAEWAGSLLPFDKPILLVTAPGQERESVIRLARVGFEKVKGCLEG
                     GFPTWLHAGEPIDMVIDVEPDELAMDIPFDGNLLVVDVRRENEYAEGHVKDAINIPLQ
                     DLADPGNMVTLEERFNLYIHCGGGYRSVIAASLLKRQGIHNLRNVTGGWKMIKEQKGI
                     PIVKDNSVLN"
BASE COUNT         1583 a         2143 c         2002 g         1430 t
ORIGIN      
        1 ctccggaccc ggaatggccc tcaagtccga ggccatgggt ctggcggtca tgctggagat
       61 ccccctcgtc atctgcgata tccagcgcgg cggcccttcc acaggcctgc ccaccaagac
      121 cgaacaaagc gatctgctgc aggcttatta tggccgtaac ggcgaatgcc cgatgccggt
      181 ggtggccgct tcgacgccga gcgattgttt tgaagccgtc tatgaagccg tccggatcgc
      241 ggtccaccat atgacaccgg ttatcttcct ttccgacggg tatattgcca acggagcgga
      301 accctggaaa tttccgaaga gcgccgatct cccgccgatc gaagtccaat tcaaaacgca
      361 gctgggcgcc gaggaagcgg tattccagcc ctatctccgc gacgagaatc tggtaaggcc
      421 ctgggcgatc ccgggtacgg ccggattgga gcaccggatc ggaggcctgg aaaaacagaa
      481 cgtcacgggc aacgtgagtt acgacccgga caaccaccag ctgatggtca agatccggga
      541 tgaaaaagtg gcccggatcg cggatcatat tccgccgcag cggctggaca gcggtcccga
      601 gaccggcgac ctgcttgttc tcggctgggg aagcacctac ggcgcaatca aaagcgcggt
      661 ggcaaccctg caaaaggaag ggcttgccgt ttcgcatgcc catctccgtt atctccgccc
      721 attcccttcc aacctgggag agatcctcca caaattcagg caggtcctga tccctgagat
      781 caataacggc cagctgatca ggattatccg cgatcagttc ctcatcgatg ccgcaggcta
      841 ccacaagatc atgggcgttc ccatcacccg gacggagctg ctggtcaaaa tgcgtgaaat
      901 gcttggcgcg aacaactaga ccgccctggg gtcccctgtt cctttcatcc ggcgttcggc
      961 ggcttcggcc gtttgttcca caggatgaag gatgacactc agcctgtaca ggtcagcgga
     1021 caccttggcg atgaaaccga attggtcttc aaccgccttg gccgccgcag gccggtcttt
     1081 ccctgaagcg ggcagtttcg gcgcctcggg cagttggagc acctggggcc tttggggcgc
     1141 aggcagcgcc tggcgcgccc cgccggccgc atggtccgcg ccgtcgacac cctcctgcaa
     1201 caccgcaacc gccgattcca acacgccgac aattccatcc gcgaccggct gccaggcctt
     1261 gccgggaaat gttgcggcaa aaggcttctc atagtaagcg agcgtggcga cataggaagt
     1321 gagcatctgg ctggccacca cgaactgatg catttccgtc atccgccgct ggcggcggcg
     1381 cggctcggat aagaccctgc cgaaggcgtc ggacagattc gccagtgcga cgaaggcatg
     1441 cttccgcgaa agcttataat cctggacgcc taccggccgg ccgaaataag cggccgccac
     1501 atcccggaag taggagatat tggcctttaa cgccgccgcc atggtatccc cgatgcgctg
     1561 atgctcccag gtagggacga tgaagatgtt ggccagaaat gcgatcgcgg aaccgatcag
     1621 cgtgtccagc acgcggtcgg caatgatgac tttgaatccc accggataga gcaggtgaaa
     1681 aagcagcagg acatagggcg tggtaaaaat gacggcgacg aggtaattcg ttcgcagaaa
     1741 agcataggtg ccgatcatga agaaaatcat tacggcaaaa agcgccggac gatcgtgaat
     1801 gaaatagatc agcagaaagc ccaggacggc cccgcaaagc gtgcccgtca gccgctggaa
     1861 gtttcgttgc ttcgtgaggc tgtacaccgg cttcaggatc acgatcaccg tcagaaggat
     1921 ccagtaaccg tggccgacgg gcaggaagcg ggaaacgata taacctgtga cggtggctac
     1981 actgacccgc aacgagtgcc tgaagatatt ggactcaagc gtaaggttgt cggtgatcag
     2041 cttccggtcc atattctgac gcgctgcgaa gcgcccatag tcgaagtcgg cgggaaaatg
     2101 cctgatcagt tgccggtcgt aggtcgtaaa ggcctgcagc acgtgcaggc gttcattgat
     2161 gtcttcgatg ctgtcgagaa tatttcccag gtcggccacc ccctcgggcg gattcaaggc
     2221 gagttttgcc cgcagctccc ggatgcggat ggacaaggtc gaccggtcca cggatggcct
     2281 gccggccatg atcgccaaac caatctcgtc cagctcactg gccaggtcga ggatcagctt
     2341 ccggcaatcg gccagaagct ggctgtcgcc gaataaccgg tgcaacagcc ggtaatcctg
     2401 ctgggaagtc atgatccgct cgaacagatc gatgctgtcg agaaagatca tcgtgaggat
     2461 ccgcccggtg agcgtcgatt cgcggacaat gttccggctc ttgaagatca gctccctcaa
     2521 aaggtcctgc tttacctgga tggacgattg gctatccagc agctgccggt agctcttatc
     2581 gtagtcgacc tgcggtgaat agaaagatgc tttgatccgc aaatagcccg ccgtcgcctg
     2641 cacgcaatcg cccaaagcct gctggataag ccggtaagga cggaaactgt agagcagcag
     2701 gctgagtccc atataccaaa ggccgccggc gagtacatac aatgcattgc ggacgacgtc
     2761 ccagccttgc acgtgatggc cgatattcag gaccatgatg agcagggcat tcagcccgat
     2821 agacgtggcc cggctgccgt aaatgcccat cagcgaaaac acaaagcaga acagcagcac
     2881 gcagcaaccc gtcaggaacg gggaggccga agcccaacct gtcaccagcg caaccacgaa
     2941 aacaaccagg atcccgaccg ccatgccatt ccggcgatga tgtattggtc ccggattgtc
     3001 cgtattgccc acgcacatgg cgccgatcga gacgacgatc ccggcggaaa gatggtgcac
     3061 caaagcaagc aaagcgcagg gcaggctgac gcctaccgtg atccggatag cggcgttcag
     3121 atagtgacta tgaataaatt tcctgtattc cctgatgtaa tccatggacg acctccaagc
     3181 atctcaaatt atgccaaacc atcgttttgc ccgcgagtct tcttaccttt accggcccca
     3241 agcgggatcc cgaatgccaa atctttatgc aagccatcat cgaagagatc aggagattgt
     3301 tccgcaccta tagtccttac ccgatcaccg gactggatgt tattccgcaa tcgggcagta
     3361 accggatcta tttccgcgtc cgcacggcgg aagacaccac gatcgccacc tataacgaca
     3421 atatccggga aaacgggact tttatctatt tcagccgcca tttccgcgaa tgcggctgcc
     3481 cggtccccga gatctacttt gtcaatgaag cgtgtacgat ctatttccag gaggatttcg
     3541 gcgaccattc cctcctgtcg gagctggaaa agtcgggaca caacgattcc gcctatgcct
     3601 ggttccgcga aagtctgcaa cagctggcca ggctccagat ccgcggcaat gagggcctgg
     3661 attacagcaa gaccattacc tcccgcgaat tcggaaaaca ggccatcctg agcgacctcc
     3721 tttatttcaa atattatttc ctggacaccc tcggcattcc ttacgacaag gaactgctga
     3781 tcgatgactt tgaaaggctg agcggctacc tggcgcaggc agagcaccgt tatttccttt
     3841 tccgcgactt ccaaagccga aacatcctgc taaaggacgg ccaggtacat tttattgatt
     3901 tccagggcgg aatgaaaggc gcgctgcagt atgacgtcgc ctccctccta tggcaggcaa
     3961 aggccgagct gtcggatccc tggaaggact ccctgctgga gcactatatg ggctgtgtcg
     4021 aagacctgct cggcgaaaag ctcgaccgcc cccaattcgt ggaccgatac aacggttatg
     4081 tcctcatccg gctcctgcag gtcctgggcg cctacgggtt ccgcggcctc ttcgaacgca
     4141 aagcccattt cctgatcagc attcccctcg ggctgcgcaa cctgcagtgg ttcgtccgca
     4201 accggcatcc cgggatcgcc ctgccggaat ttgaccggat cctgcaactc gtggtccgcg
     4261 acgaagtgat ccatcgtttc gaaccggtgc aggcggatca ggattcaccg ctcgtcatcc
     4321 atatccgaag cttttcctac cggaaaagta tacccgcgga cgaaagcgga aacggtggcg
     4381 gatttgtgtt tgattgccgc ggcatcctga accccggacg gatcctggaa ttcaagtccc
     4441 tcaccggtcg cgataaggcg gttaaagact atctcgaaca acagaccagg atgccggagt
     4501 tcctgaacag cgcatacggc atcgtcgata tctccgtgga agactacatt cagcgggggt
     4561 tctccagcct gtgcatcagc ttcggctgca ccggcggcca gcaccggagc gtctatgcgg
     4621 cagacgcgct ggcgaggcat ctgcggaaca aatatcatgt gaaggtcgac ctgcagcata
     4681 tcgagcagga agcgaaaaac tgggagaact gatgtattac ctggtctatg gcttgttgta
     4741 cgggatctcg ctgctgccaa cgtggatatt gtacggcgta tcggacgcca tggcgctgac
     4801 gctgtacgcc gtagcccggt accgccggaa aattgtgctg gataaccttc ggctcgcctt
     4861 cccggaaaag acggaggcgg aacgcctgcg catcgccaaa gaattttacc gcaatttcac
     4921 cgacagcttc gtggaaacga tcaagctcat ctccgccggc ccagcctggc tggaaaaaca
     4981 ctttgtgatc gacaacccgg aagttatgga agacttcgcg cgggaagggc ggaagatcca
     5041 gctattcctc ggccacctgt tcggctggga ggttgcgaac gtcgccatgc cgcggctcac
     5101 gcgcttcaag tttatcgttg tgtatatgcc cgtaacgaac aaaaccttcg agcggctgtt
     5161 cctccggctg cggggtcgca ccggcacagt gttgttgcct gctacccaac tgcaacgggc
     5221 catccttcct tatcgcaata ccgtctacgc cctcgcgctc gtcgccgacc aggcgcccgg
     5281 cggacccgag aactcctact ggctgaactt cttcggaagg ccgacggcct ttattcgcgg
     5341 cgccgaacgc ggggcaagga ccggcgacat cccggctgta ttcgcccgcg tttataagac
     5401 gggaaggggt ctttaccgct cccgcctgac gctcatgacc gagcggccgg cagcgcttcc
     5461 cgaaggcgag ctcacgcggc gataccgtga cctgatggaa accgccattc gtgaacagcc
     5521 tgccacctgg ttgtggagcc acaaacgctg gaaatttgag tggaatgaaa ccctggcgcc
     5581 tttctggatc gacgggcaaa aaatttaatc ctgcagcggg acaagcccgt cttgcgattc
     5641 aggaatttat aatacattta cgaagcatcc tgcatttttt cccaccgcct ccgtgcccgg
     5701 tggttggctt aaggtttcct gattagcagc agaaccaaat aaacctaaag ctcaacctca
     5761 accggatgaa aaattctaaa cctggagttg cattgatcga agatcgcgtg ttgctccact
     5821 gcagaccggt actggtcctg aaggaccggg aactcgaatt catgcgcctg gtttgcaccg
     5881 aatgcactta caagcagatc gccgacagaa tgtacctcag ccccaggacc atcgatggtt
     5941 accgcgatgc cttattcgaa aagttgaacg ttcgcacgcg ggtcggactg gccatgtttg
     6001 cggtccgtac cggcatcgtg catgtcgctt ccctcccggc aagcgatgta tgggatgaaa
     6061 agcaggcaac cccggacaga agggaacgca gggcggcgag acttgccggc cgctgattat
     6121 ttctagttca gcacgctatt gtcttttacg atcggaatac ccttctgctc cttgatcatt
     6181 ttccatccgc ctgtcacatt tcgcaggttg tggataccct ggcgtttgag cagggacgcc
     6241 gcaatgacgc tccggtaacc gccgccgcag tggatgtaaa ggttgaaacg ctcttccagg
     6301 gtcaccatgt tcccgggatc tgccaggtcc tgcaacggga tattaatggc atccttgaca
     6361 tgcccttccg cgtactcgtt ttcgcgccgg acatccacca ccaacagatt cccgtcaaaa
     6421 ggaatgtcca tcgccagctc atccggctcc acgtcaatga ccatatcgat cggctcaccg
     6481 gcatgcagcc atgttgggaa acccccttca aggcagccct taaccttctc gaagcccacc
     6541 cgcgccaggc ggataacgct ctctctttcc tggccgggtg ccgtgaccag gaggatcggc
     6601 ttgtcgaaag ggaggaggct gccggcccac tcggcgaacc tgccgtccag gccgatgctg
     6661 atggatccgg ggatgaatcc ggctacaaac gtcgccgctg ggcgggtgtc cagcagaata
     6721 gccccctctt cgcccatccc tcttttgaag gtctcgaggt caagcggctt cagcccgttc
     6781 gcgaggacct cttccaggct gccgtagccc tcccggttga tgcgcgcatt gatggaaaaa
     6841 taagaaggcg gcgcatcgag gccgtccgtc actgcgcgga caaattcttc ttttgacgcc
     6901 gcccggaggg catagttggt ttccttctcg tccctcaatg tactctgcgt gcctggcccc
     6961 agattttttc cacagctgct gccgggcccg tgcgccggat agatgacggt attttcaggc
     7021 aagggtaata tcctggtctg gagggagtca tacagcatcg acgccaactc ttcgcgggac
     7081 aggttgccgc tgctcaggtc gggcctgccg acatcgccga cgaagagggt gtcgcccgtg
     7141 aacagcaacg gctcggcc
//
LOCUS       DATGKE010000710         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_122424, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000710 DATGKE010000000
VERSION     DATGKE010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122424"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..742
                     /locus_tag="VKU83_12070"
     CDS             44..742
                     /locus_tag="VKU83_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI94346.1"
                     /translation="MLTCIAVDDEPLALEVLKKYIAKIYFLDLRGLYTDPFEAQKAMD
                     VSPVDILFLDIQMPDINGIEFSRIINKKNTAVVFTTAFSEYAVEGFNVDAIDYLLKPI
                     EYDRFLKAVYKAKEYVDYLTNQELQEGYIFVKSDYQMVKVNLKEILYIEGLDDYIKIY
                     LPGKSILTLMTLKTIAQKLPAREFVRAHRSYIVPISKIEHISKSKIRIADKEIPIGVS
                     YSESFFAAMENKLN"
     gene            951..2474
                     /locus_tag="VKU83_12075"
     CDS             951..2474
                     /locus_tag="VKU83_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLI94347.1"
                     /translation="MNFLEELMVRSENPGASTGAEWLSSGGKPLESYSPVDGKRIASM
                     TLTDKAAYDQVVDTAQRAFSLWRTWPAPRRGEVVRQIGMELRKKKQALGRLVSYEMGK
                     SLQEGYGEVQEMIDICDFAVGLSRQLHGLTMHSERPGHRMYEQWHPIGIVGIISAFNF
                     PVAVWSWNSMIAWVCGDVCIWKPSERTPLCALACQRIVAEVFAANQVPEGVSGLITGA
                     RDAGEWMAADHRIPMISATGSTRMGKAIGTAMAARLGRALLELGGNNAIIISQDADLD
                     IAIPGAVFGAVGTAGQRCTTTRRLIIHESIYDKVKDRMVAAYRQLRIGDPLQESNHVG
                     PLIDKAAVQAYEKAVEAGKDQGCRFLTGGKVLAGDGFESGCYVTPAIAEAEPGYPIVQ
                     EETFAPILYLMRYKTIDDAIQIQNDVPQGLSSSIMTLNLREAEQFLSAAGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKSYMRRQTNTINYSHQLPLAQGIRFEI"
     gene            2563..>3515
                     /locus_tag="VKU83_12080"
     CDS             2563..>3515
                     /locus_tag="VKU83_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HLI94348.1"
                     /translation="MRNILLLWLLTMGAVTAQAQARLGILGGLHSSNILETNHLPGWD
                     TAVRKYLDPRSGFELGLIAEVPLGRKGFYFQPGIIYTTKGRNYDRNNDSVKSLQTDTI
                     YNKTGLNLDYIEIPLNLTYKFALTPNGKNSLFISAGPYMSFFYSGNLTVSSLTTNPEQ
                     YNSQTAPVTVGKGPESYTTTDLGVSGKLGFELGTIMISSYFSRGLTSIYHADYPGSFH
                     HTIFGASLGIWLSSAGTPPPVRVKDTDKDGIPDEQDMCPLKPGIAKYHGCPVPDTDHD
                     GIDDEHDSCPNVPGLARYHGCPIPDRDGDGVNDEQDQCPDSA"
BASE COUNT          821 a          957 c         1012 g          725 t
ORIGIN      
        1 atcgaaacaa tgaatttagc gttaacctaa atatccccgt ctgatgttga cctgtatcgc
       61 agtcgatgat gagccgctcg cgctggaagt gttgaagaaa tacatcgcga agatttactt
      121 cctagacctc aggggcctgt acacagatcc ctttgaagcg cagaaggcga tggatgtttc
      181 cccggtggac atccttttcc ttgacatcca gatgccggat atcaatggca tcgaattctc
      241 ccggatcata aacaaaaaaa atacggcagt ggtctttacg accgccttta gcgaatacgc
      301 ggtggaaggg ttcaacgtcg atgcgatcga ctatctgctg aagccgatcg aatatgaccg
      361 gttcctgaag gccgtgtata aggcaaagga atatgtcgat tacctcacca accaggaatt
      421 gcaggagggg tatatcttcg tcaaatccga ctaccagatg gtgaaggtga acctcaagga
      481 gatcctttac atcgagggcc tcgacgacta tatcaagatc tatcttcccg ggaaatccat
      541 cctcacgctg atgacattga agacgattgc ccaaaaacta cctgcgcggg aattcgtccg
      601 ggcgcaccgg tcatatatcg tgcccatcag taagatcgaa cacatcagca agagcaagat
      661 ccggattgcc gacaaggaaa tacctattgg cgtcagctac agcgagagtt tctttgccgc
      721 catggaaaat aagctgaatt gagggccgcg ggatcgccgg gcggtccgtt gacgccgccc
      781 gcccgccagg caaccttttc acgcagattt tcgtatcgat tattgccggt aaggtagatg
      841 ccggcatagg accagtttct tcatagtaga ttttaagtta aggttgagga cccggttagt
      901 ggccggtttt cggtaaattg cttgcgattc attaatcgca agcttttttt atgaatttcc
      961 tggaagaatt gatggtcagg tccgagaacc cgggcgcctc gaccggagcg gaatggctca
     1021 gctccggcgg aaagccgctg gaatcctact ccccggtcga tggaaaacgc atcgcgtcca
     1081 tgacgttgac cgacaaggcg gcctatgatc aggtggtgga tacggcgcag cgcgccttca
     1141 gcttgtggcg gacatggccg gcgccccgcc gcggcgaggt cgtgaggcag atcggcatgg
     1201 agttgcggaa aaagaagcag gcgctcggcc gcctggtttc ctatgaaatg ggcaagagcc
     1261 tgcaggaggg atatggcgag gtccaggaga tgatcgacat ctgcgatttt gccgtcgggc
     1321 tttcccgcca attgcatggc cttaccatgc acagcgagcg tcccggccac cgcatgtatg
     1381 aacagtggca tcccattggc atcgtgggta tcatctccgc ttttaacttt cctgtcgccg
     1441 tgtggagctg gaacagtatg atcgcctggg tttgcgggga cgtctgcatc tggaagccgt
     1501 ccgagagaac accgctctgc gcactcgcct gccagcggat cgtagcggag gttttcgcgg
     1561 cgaaccaggt tcccgaaggg gtgagcgggc tgatcaccgg cgcccgggat gccggcgaat
     1621 ggatggcggc tgaccaccgc attcccatga tctcggccac agggtccacg cggatgggaa
     1681 aagcgatcgg caccgcaatg gccgcaaggt tgggaagggc gctcctggag ctgggaggca
     1741 ataacgcgat cattatctcc caagacgcgg acctggacat cgctatcccc ggcgccgtat
     1801 tcggggcggt cggaacggct ggccagcgtt gcacgacgac caggaggctc atcattcatg
     1861 aatcgatcta tgacaaggta aaggacagaa tggtcgccgc ttaccggcag ttgcggatcg
     1921 gcgacccgct ccaggagtcg aaccatgtcg ggccattgat cgacaaagct gcggtacagg
     1981 catacgagaa ggcggtagag gctggaaaag atcagggctg ccggtttctg accggcggaa
     2041 aggtgcttgc cggagacggt tttgagagtg gctgttacgt tactccggcg attgcggagg
     2101 cggagcccgg ataccccatc gtgcaggaag agacttttgc tcccatcctc taccttatgc
     2161 gctataagac gatcgacgat gccatccaga tccagaacga cgtgccgcaa ggactgtcct
     2221 cctccataat gacgctcaat ctccgggagg ccgaacaatt tctctccgcc gcaggcagcg
     2281 actgtgggat cgccaacgtg aatatcggga cgtccggcgc cgagatcggc ggcgccttcg
     2341 gcggcgaaaa ggaaaccggc gggggaaggg aaagcggcag cgatgcgtgg aagtcctata
     2401 tgcggcgcca gacaaatacc atcaattact cgcatcaact gcctttggca cagggaataa
     2461 ggttcgagat ttgatttttt ctcaataata gggtcgaatt aagcgttata gagggcagat
     2521 tccaatacca tgaatgccgg ccttaacgcc ccttccgctg ttttgagaaa tatcttgctg
     2581 ctttggctgc tgaccatggg cgcagtaact gcgcaggccc aggccaggct ggggatcctc
     2641 ggcggtttgc actcctctaa cattttggaa accaaccatc tacccggttg ggacacggct
     2701 gtcagaaaat atctcgaccc gcgcagcgga ttcgaactgg gactgatcgc cgaggtgccg
     2761 ctcggcagga aaggctttta tttccagccc ggtattatat acaccaccaa gggccggaat
     2821 tacgaccgca acaacgactc ggtcaaaagc ctccagaccg ataccattta caataagacg
     2881 gggctgaacc tcgactacat cgagattccg ctgaatctga cctacaagtt tgcgctgaca
     2941 cccaatggga agaacagcct ttttatcagc gccggcccct atatgagctt cttttactcc
     3001 ggcaatctga ccgtatccag cctgaccacc aacccggaac agtataatag tcagacggct
     3061 cccgtgaccg tgggaaaggg tccggaaagt tacacgacca ccgatctggg cgtcagcggc
     3121 aaactgggct tcgagctggg cacgatcatg attagcagct atttcagccg cggcctgaca
     3181 agcatctatc atgcggacta ccctggcagc ttccatcata ccattttcgg ggcttcgctg
     3241 ggtatctggt taagctcggc cgggactccc ccgccggtga gggtaaagga cacggacaaa
     3301 gacgggattc ccgacgaaca ggacatgtgt cccctgaagc ccggcatcgc gaaataccat
     3361 ggctgccccg tgccggatac cgaccatgac gggatcgatg acgagcacga ttcctgcccg
     3421 aacgtgcctg ggctggcccg gtatcacggc tgccctattc ccgaccggga tggggacggt
     3481 gtcaatgacg agcaggatca atgcccggac agcgc
//
LOCUS       DATGKE010000711         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_463557, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000711 DATGKE010000000
VERSION     DATGKE010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463557"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2241)
                     /locus_tag="VKU83_12085"
     CDS             complement(<1..>2241)
                     /locus_tag="VKU83_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94349.1"
                     /translation="TWRRVLYVNDRTACSEVVMEPGNPDVLYAGMWRVDRTPYSIESG
                     GEGSGLYKSTDGGETWANISSHRGLPKGIWGNVNIAVSASDPDKVFAMIENASGGLYL
                     SKDRGDTWTKLNGDNDIKQRAWYFNRVYVDPRNENLVYCLNVELERSLDGGKTFAVLP
                     SPHSDHHDLWIDPSDGRRLILADDGGAQVSFDGGADWSTVENQPTAQIYRVTTDNAFP
                     FHILGAQQDNTSVRIKSRTAGAAITARDWEPTAGFESGYIVPDPLNPDIVYGGNYDGY
                     LGRLNHRTGENRAIDVWPDNPMGSGDDSLKYRFQWNYPIFFSPNNPHRLYAAGNCLFE
                     TENEGESWKQISPDLTTNDKSKQGPSGGPITKDNSSAEYYCTIYTAAESPLEPGLLWT
                     GSDDGLIYVSRDSGTHWENVTPPAAGKWTLWNCVEPDPFRKGSAYFVGTRFRLDDYTP
                     YIFKTEDYGKTWKLITNGIDPMHFARVVRADKKRPGLLYAGTEYGMYVSFDDGAHWKP
                     MQLNLPIVPVTDMTIKDNSLVVATQGRAFWVLDDLGLVQQLQADSLRPRLHVFAVEDA
                     WRFGSGGGRRNRDRAISNAGQNPPSGVVVRYWLKDAPDTPRVTITVFDKKHEPIRTFS
                     THSKESDTKLECHDGINTFVWDMLYPPAVKVPGMLLWTGGAGTPMAAPGRYSVRVRYG
                     HDSAEVPFVIKADPNFSMTEADYDAQVGFLLQVRDKYNAVQRAVLQIRSIRGQLQALN
                     GRLDST"
BASE COUNT          428 a          704 c          617 g          492 t
ORIGIN      
        1 tgcgtgctgt cgagccggcc attcaacgcc tgcaattgtc cgcggatgct ccggatctgc
       61 agcaccgccc gttgcacggc attatactta tcccgcacct gcagcaggaa accgacctgg
      121 gcgtcgtagt ccgcctctgt catggagaag ttcgggtccg ccttgatgac aaaggggacc
      181 tccgccgaat catgtccgta ccgcaccctg accgagtacc ggccgggagc cgccatcggc
      241 gtgcccgcgc cccccgtcca aagcaacatg cctgggacct tgacggccgg cggatagagc
      301 atatcccaca caaatgtatt gatgccgtcg tggcattcca gcttggtatc ggattccttc
      361 gaatgcgtgc tgaaggtccg gatcggttcg tgcttcttgt caaacacggt aatggtcacc
      421 cgaggagtgt ccggcgcatc ctttagccag taccgcacca cgacacccga aggagggttc
      481 tgacccgcat tggagatcgc ccggtcccgg ttgcggcgac cgccgccgct cccgaaccgc
      541 catgcatcct ctaccgcaaa tacgtgcagc ctgggccgaa gggaatcggc ctggagctgc
      601 tgcaccagac ccagatcgtc gaggacccag aatgcccggc cctgggttgc cacgacaagg
      661 ctgttgtctt tgatggtcat gtccgtcacc ggcacgatag gaaggttcag ctgcattggc
      721 ttccaatgcg cgccgtcgtc gaagctgacg tacatcccgt attccgtgcc ggcatagagc
      781 aagccggggc gcttcttgtc cgcccggaca acccgcgcaa aatgcatggg atcgatccca
      841 ttggtgatca gcttccaggt cttgccgtaa tcctctgttt taaagatata aggggtgtag
      901 tcgtccagcc tgaaacgcgt cccgacaaaa taggccgagc cctttctgaa aggatcgggt
      961 tcgacacagt tccacaatgt ccatttgccg gctgcggggg gtgtcacatt ctcccagtgc
     1021 gttccgctat cccgggaaac atagatcaag ccatcgtcgc tgcccgtcca caacagccct
     1081 ggttcaaggg gggactcggc ggccgtatag atcgtgcaat aatactcggc ggaggagttg
     1141 tccttcgtga tggggccgcc actcggtccc tgtttcgatt tatcgttcgt cgtcaggtcc
     1201 gggcttatct gcttccagct ctcaccctcg ttttctgtct cgaaaagaca gttccctgcc
     1261 gcatacaagc gatgcggatt gttcggcgaa aagaagatgg gataattcca ttggaagcgg
     1321 tacttgagcg agtcgtcgcc cgaccccatg gggttgtccg gccacacatc gatcgcccgg
     1381 ttctcacccg ttcggtgatt cagccgcccg aggtagccgt cgtaattccc gccgtagacg
     1441 atgtcgggat ttaatggatc cgggacaata tatcccgact cgaaacctgc agtcggttcc
     1501 caatcccttg cagtgatggc ggctcccgcc gtccggcttt tgatccggac actggtattg
     1561 tcctgctgcg cgccgagaat atggaacggg aaggcgttgt ctgtcgtcac ccggtaaatc
     1621 tgcgccgttg gctgattttc caccgtgctc cagtcggcgc cgccgtcgaa gctgacctgt
     1681 gcgcccccgt catccgccag tatcaaccgg cgcccgtccg agggatcgat ccataggtca
     1741 tggtggtcgc tgtgaggcga aggcaggact gcaaacgtct tgccgccatc aaggctccgc
     1801 tccagctcca cattcaggca ataaacgaga ttctcgttcc tgggatcaac ataaacccgg
     1861 ttgaaatacc aggcgcgctg tttgatatcg ttatccccgt tcagtttcgt ccaggtgtcg
     1921 ccgcggtctt tgctgagata taggccgccc gaagcgtttt cgatcatggc aaacacttta
     1981 tccggatcag acgccgaaac ggcgatgttg acgtttcccc agattccctt gggcaaaccc
     2041 cggtgactgg agatattggc ccaggtctct ccgccgtccg tgcttttgta cagtccactg
     2101 ccttccccgc cgctttcgat actataaggc gtgcggtcca cccgccacat ccccgcatac
     2161 aacacatcgg ggttgcctgg ctccatgacc acttccgaac aggcggtccg gtcgttcaca
     2221 tatagaaccc gccgccatgt c
//
LOCUS       DATGKE010000712         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1552630, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000712 DATGKE010000000
VERSION     DATGKE010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552630"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..617
                     /locus_tag="VKU83_12090"
     CDS             117..617
                     /locus_tag="VKU83_12090"
                     /inference="COORDINATES: protein motif:HMM:NF039704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HLI94350.1"
                     /translation="MKKQSFYTLLFMTIGLCACHKETSIEKGSGLAADMVATIGAQPW
                     EAADSAQAAIFSHGMAIISGTSADGQEISITLNDTVVGLYSLNQSSTSLAVYANLDSV
                     GSYAFSTNQGVDTSQAGGTVNVISVDPVKRLISGVFSFKVYRDQDNSQRNITQGVFYD
                     IPYTDQ"
     gene            850..1242
                     /gene="rnpA"
                     /locus_tag="VKU83_12095"
     CDS             850..1242
                     /gene="rnpA"
                     /locus_tag="VKU83_12095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLI94351.1"
                     /translation="MGSHRSFTWNKKEKLKSRKRIERVFREGKSMSVFPFKIYWILDS
                     VPAESPLQAGFGVSSRHFKNAVDRNRIKRLSREAYRLLKPPLLQRLKEAGAWMAVFFV
                     YTGKDLPEFATVSKQIGVALQKLTKELS"
     gene            1239..1469
                     /gene="yidD"
                     /locus_tag="VKU83_12100"
     CDS             1239..1469
                     /gene="yidD"
                     /locus_tag="VKU83_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLI94352.1"
                     /translation="MKAFLRILGFPVIALIRIYQWVLSPLLGPKCRYVPSCSQYAIEA
                     LKKHGLFKGGWLAIRRISRCHPWGGHGVDPVP"
     gene            complement(1472..>2071)
                     /locus_tag="VKU83_12105"
     CDS             complement(1472..>2071)
                     /locus_tag="VKU83_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94353.1"
                     /translation="QTLNMKRLPGLLVLTALCSLSACKGHEKAIMIYADSDIRIDDTQ
                     QHITVSDGTTHHEQELEFHSSSPVTLTVQSPQGKFDFTATDDGLYILNLKTDTVVGSY
                     KHVGENAVTHISQEVAKHDLDSLQKLVANQNVSDANRNYFIVPDKMVRISANPKGKIF
                     GPFRSIPGSFDAGSVPEIYKFYNISEVREIIDNLEKVTK"
BASE COUNT          466 a          527 c          573 g          505 t
ORIGIN      
        1 caggggacaa ggcagttctt tacgacggac tcgacggaag attttgatgc ccatgcgcgg
       61 attttttacg gtgaggagtt gagatcctca cccgtattgc taaatggaat gcccggatga
      121 aaaaacaaag tttttatacc cttttgttca tgacgatcgg gctgtgcgcc tgccacaaag
      181 agaccagcat cgaaaagggc tccggcctgg ctgccgacat ggtggcgacg atcggggctc
      241 agccatggga ggcggcggac agtgcccagg cggcaatctt ttcgcatgga atggcgatta
      301 tctcgggtac cagcgcggac ggccaggaaa tcagcattac actgaacgat acggtcgtcg
      361 gtttatacag cctgaatcag agctcgacct ccctcgccgt ttatgccaac ctggattcgg
      421 tgggatccta tgctttttcc acgaaccagg gtgtcgatac ctcccaggcg gggggaaccg
      481 taaatgtcat ctcggttgac ccggtaaaaa ggctgatttc cggggtattt tccttcaagg
      541 tataccggga ccaggacaat agccaacgca atattaccca gggcgtattt tacgacatcc
      601 catatacgga tcaataaagc ggctgccgga ccggttttga taatttgccg gttttttcct
      661 acatttgcgt cccaaacgaa tttttaaatg aagcgcacat ttcaaccgca cagacgtcgt
      721 cgcaagagcg ttcacggttt ccgcgagcgg atgcagactg ccaatgggcg gaaggtcctg
      781 gccagccgcc gcagaaaagg ccgtaagaag ctgaccgttt ccgacgaatc gagactaaag
      841 tagaccatca taggtagcca tcgttcattt acctggaaca agaaggaaaa gctcaagagc
      901 cgaaaacgga tcgagcgcgt ttttcgggaa gggaagagca tgtccgtttt tccatttaaa
      961 atctattgga tccttgactc cgtcccggcc gaatcaccct tacaggccgg ttttggcgtc
     1021 agcagccgcc attttaagaa tgccgtagac cgcaaccgga tcaaaagact gagcagggaa
     1081 gcctaccggc tcttgaaacc acctctttta cagcggctga aggaggcagg ggcgtggatg
     1141 gcggttttct ttgtgtatac cggtaaagac ctgccggaat tcgccaccgt aagcaagcag
     1201 atcggcgtag ctttgcaaaa gcttacgaag gaactatcat gaaagccttt ttgcggatat
     1261 tgggttttcc ggttattgca ctcatcagga tctatcaatg ggtgctttcg cctttgctgg
     1321 gaccgaagtg ccggtatgta cccagctgtt cacaatatgc catcgaggcc ctgaaaaaac
     1381 acgggctgtt caagggcggc tggctggcga tccggcggat ttcccgctgc cacccctggg
     1441 gcggtcacgg cgtggacccg gtgccttgaa tctatttcgt tactttctcc aggttgtcga
     1501 tgatctcccg cacttccgag atattataga atttatagat ctccggcacc gatcccgcgt
     1561 cgaatgaacc cgggatggac ctgaacggcc cgaaaatctt tcctttagga ttagcgctga
     1621 tcctgaccat cttgtcgggg acgataaagt agttgcgatt ggcgtcgctg acattctggt
     1681 tcgcaacgag cttctgcaag ctgtcgaggt catgcttcgc cacttcctgc gagatatgcg
     1741 tcacggcatt ttcgcctacg tgcttatagc tgccgacgac ggtatccgtc ttcaggttca
     1801 ggatatagag gccgtcgtcg gtggcggtaa agtcgaactt accctgcggc gactggacgg
     1861 ttagcgttac ggggctgctg ctgtggaatt cgagctcctg ctcatgatgg gtggtcccgt
     1921 ccgagacggt aatatgctgt tgggtatcgt cgatcctgat atcgctgtcg gcgtagatca
     1981 tgatcgcttt ctcgtgaccc ttgcatgcgc ttaggctgca gagcgccgtt agtacgagga
     2041 gacctggcaa tcttttcata ttcagtgttt g
//
LOCUS       DATGKE010000713         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_364172, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000713 DATGKE010000000
VERSION     DATGKE010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364172"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VKU83_12110"
     CDS             <1..340
                     /locus_tag="VKU83_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HLI94354.1"
                     /translation="YAVAVLVMFLIGAPLGSIIRKGGIGMPLVFAVIFFVIFFLLNNF
                     GRKFTKEGVMGPIGGMWLATYVLTPIGLFLTYKALHDSQLFNKEFYFRISRGIRKFIR
                     KMRNARQPAA"
     gene            403..1404
                     /locus_tag="VKU83_12115"
     CDS             403..1404
                     /locus_tag="VKU83_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLI94355.1"
                     /translation="MSKTSENIGLQRWVAIIAILLFLLKLAAYYLTQSVAVLTDALES
                     IVNVLAGLIGLYSLRIAAKPSDQDHPYGHGKAEFLSAAVEGSLVIMAGLIIIYEAVID
                     LVHPAPIQKLDKGIYIVAFTAVANFIVGTICVYRGRRNNSMQLVAAGKHLQTDTWNTT
                     GIIVGLILIYFTKAPWLDGLVALLFAALILYTGYHILRKSVAGIMDEADLKLLHDLIS
                     YLNAHRRENWIDLHNLRVIKYGDGLHIDCHLTVPWYLTVLDAHAEIDAFTSLVRGRFG
                     EQIEFFVHTDPCLEFSCRICTKESCGVRKHPFEERVPWTLLTVVPNKKHESTKAYLA"
     gene            1443..>2400
                     /locus_tag="VKU83_12120"
     CDS             1443..>2400
                     /locus_tag="VKU83_12120"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI94356.1"
                     /translation="MSTWQTYQAQHKERFLSELLDLLRIPSVSARSEHKPDMQKCAAA
                     VRQSLLQAGVDKAEVYPTEGHPVVYAEKIIDPKMPTVLVYGHYDVQPVEPLNLWNSGP
                     FEPVIKDGKIYARGSADDKGQFFMHVKAFETMARTGTLTTNIKFIIEGEEEIGSPSLG
                     RFVAAHRDLLKADVILISDTSMLGLDKPSIDIGVRGLSYIQVEVTGPNRDLHSGVYGG
                     AVANPITILAKMIAACHDGNNHVTIPGFYDQVLEVPAAERKLLAQAPYDEHEYMQDLG
                     VNALWGEKGYSTNERTGIRPTLELNGIWGGYTGEGSKTVLPSK"
BASE COUNT          518 a          729 c          632 g          521 t
ORIGIN      
        1 ctacgccgtc gccgttttgg tcatgttcct tatcggcgcg cccctgggat ctattatccg
       61 aaaggggggc atcggcatgc cgctcgtatt tgcggtcata ttcttcgtga tcttcttcct
      121 cctcaacaat tttggccgca agttcaccaa agagggggtc atgggtccca ttgggggaat
      181 gtggctggct acttatgtgc tcaccccaat cgggctcttt cttacctaca aggccctgca
      241 cgactcgcag ctcttcaata aggagtttta tttccggatc agccggggta tccggaagtt
      301 cattcgaaag atgcgcaacg cgcgccagcc ggccgcctga gcctgcgttc ggcgccagcc
      361 ggaaaaagta aatcagattc cctatttttg gcgccattgc cagtgtcaaa aacctccgaa
      421 aatatcggcc tgcaacgatg ggtggcgatc atcgccatcc tcctctttct gctcaagctg
      481 gccgcgtact acctgaccca atcggttgcc gtcctcaccg acgcgttgga aagcatcgtc
      541 aatgttcttg cgggcttgat cgggctctac agcctccgga tagccgccaa acccagcgat
      601 caggaccatc cctacggcca cggcaaggcc gaattcctct ccgccgccgt cgaaggctcg
      661 ctcgtcatta tggccggcct gatcatcatt tacgaagccg tcatcgacct ggtccatccg
      721 gccccgatcc agaaactgga caaaggcatt tatatcgtgg ccttcaccgc cgttgccaat
      781 ttcatcgttg gaacgatctg tgtttacagg ggccggcgta acaactcgat gcaactcgtc
      841 gccgcgggaa agcatctcca gactgatacg tggaatacta ccggcatcat cgtcggcctg
      901 atactcattt atttcacgaa ggcgccctgg ctggatgggc tcgtcgccct cctattcgcc
      961 gctctcatcc tgtacaccgg ctatcatatc ctgcgcaagt ctgttgcggg tatcatggat
     1021 gaggcggacc tgaaactgct gcatgacctc atctcctatt tgaatgcaca tcgccgcgag
     1081 aactggatcg atctgcacaa cctgcgcgtc atcaaatatg gcgacggcct gcatatcgac
     1141 tgccacctga ccgtaccctg gtatctcacc gtcctggatg cccatgccga aattgacgcc
     1201 ttcacctctc ttgtgcgcgg taggtttggt gaacagatcg agttttttgt ccataccgat
     1261 ccctgcctgg aattctcctg ccgcatctgc acgaaagaaa gctgcggggt ccgcaaacat
     1321 cccttcgaag agcgggtgcc gtggacgctt ttgacggtcg tgccgaacaa gaagcatgaa
     1381 agcacgaagg cttaccttgc ctaatttgat ctatctttgg caccctaaca aaatagtcta
     1441 cgatgtccac ctggcaaacc tatcaggctc agcataagga gcgtttcctc tccgagctcc
     1501 tcgatctcct ccgcatcccg tcggtaagcg cccgcagcga acacaagccc gacatgcaga
     1561 aatgcgccgc ggcggttcgg caaagcctgt tgcaggccgg cgtcgataaa gccgaggtct
     1621 acccgaccga gggtcatccc gtcgtgtacg cggaaaagat tatcgaccca aagatgccca
     1681 cggtactcgt ctatggccac tacgatgttc aaccggttga acccctgaat ctctggaaca
     1741 gcggcccctt cgagccggtc ataaaggacg ggaagatcta cgctcgcggc tcagccgacg
     1801 ataaaggcca gttctttatg catgtgaagg cgttcgaaac gatggcgcgg acgggcaccc
     1861 tcacgaccaa tatcaaattt atcatcgaag gcgaagaaga gatcggtagt cccagcctcg
     1921 ggagattcgt ggcggcgcat cgcgacctcc tcaaagccga cgtcatcctc atcagcgaca
     1981 cttcgatgct cggtctggat aagccttcga tcgacattgg cgtccgcggg ctctcctata
     2041 tccaggtcga ggtcaccggg cccaaccgcg acctgcacag cggggtgtat ggtggggccg
     2101 tcgcgaatcc catcacgata ctggcgaaga tgatcgcagc ctgtcatgac gggaataacc
     2161 atgttaccat cccggggttt tatgaccagg tactggaagt gccggccgct gagcggaaac
     2221 tgctcgcgca ggcgccgtac gacgaacacg agtacatgca ggatctcggg gtcaatgcgc
     2281 tttggggcga aaaaggttat tccacgaacg aacggaccgg catccggccc acgctcgagc
     2341 tcaacggaat ctggggcggc tataccggcg aaggcagcaa gaccgtcctc ccttcgaaag
//
LOCUS       DATGKE010000714         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1326439, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000714 DATGKE010000000
VERSION     DATGKE010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326439"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VKU83_12125"
     CDS             complement(<1..1163)
                     /locus_tag="VKU83_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94357.1"
                     /translation="MFRKNHTVKINLPGGIVATGDLYAIVAAAEQARVGEMQLGARQQ
                     LYCKAVDRYGPDLLQSLGAAGVFFETGKETFPNVVSSYVTEGVFGKSAWVSEGLYKDI
                     LEGFDHRPAVKINLVDGGQSFVPFFTGHLNFVSSSMTHHWHLFLRRPGTNEVFRWRTA
                     VYSADIARVSKLLEEEMGSPGFVKARPAEAGETWGDRLYHRVSIERSFPRGPAAPEWR
                     LPPFRLPYYEGFNRYGDKYWLGIYRREEMFPVAFLKDLCLLCRETKIGQLYSTPWKSL
                     LIKGIEEDDLPLWEFVLGKYRMSVRHAANELNWQVEDLSPDGLRLKRYLVRQLDQDDI
                     RTEGLSFAVKLQRGSGLPGSIVIRKQAEIRPGQWKALDRYELWHTVNFDPHSR"
     gene            complement(1153..>3814)
                     /locus_tag="VKU83_12130"
     CDS             complement(1153..>3814)
                     /locus_tag="VKU83_12130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLI94358.1"
                     /translation="IRLAAEWIATARGWISFWTMGLNQSARGVDKVLSLIDLHLLTGQ
                     IGRPGCGPFSLTGQPNAMGGRETGGLANLLPAHRELSNPAHRAELQKFWGSGPICPEP
                     GLTATEMFDALSQGRMKAIWIIGTNPLVSLPDARKAEQALRNARFVVVQEISNRPETL
                     GCADIILPAAGWAEKQGTMTNSERRISLLDKVVDPPGEARPDAAIICALAEKMGFPGF
                     DYPDPAAIFREHALLTAGTSIDISGLDFEILRARRSVQWPYPPGQAGEGTARLFGDHL
                     FPTPTTRAVFHAVSDDIRSERPDAEYPFILTTGRIRDQWHTMTKTGKVNKLKLHSPQA
                     FLEMHPADAGQLGICEGEIVAVISRRGDVRVPIRLSESIKRGVVFLPMHWGKLLNSDL
                     NRANNLTNSLVDPVSKEPDYKYSAVRVDKHKKTKERIVIVGAGAGARGFIHSYRALNN
                     EDEIIVFNKENEPFYNRVQLPEYINGSQTWAQLVRMTDEEEARLQIRLHRGVSIVKID
                     RAGKSVTDSTGAVTRYDKLILAMGSRAAMPKQVPALQGIFTLRSRADADRFRAELAEG
                     SRVIVVGGGLLGLELAASLREMGIAVSIVHRSSRFLDRQLDRLGSQLLHEEIVDQGCE
                     VYYSDEVQLFRGKSRLTGVQLRSGRQLEGDALVFAIGTVPNIDIAKDAGLEYHRGVVV
                     DGYLRTSDPDVYAIGEIAEFQGSLFGFTAAAEQQAGIVARYLHGDIASYYSGTVPMNI
                     IKIHGFDLCSIGLAESPDSRDYEEIVFIDKARRYYKKCIIHEDRMVGAILIGDKAEFN
                     EFRDWITRRIELSDKRLQLLRNGGRTEPVQGRLVCSCNHVGSGNLKKKVAEGCRDVAE
                     LCSITGAGTGCGSCRPEVIKIMAEALADVS"
BASE COUNT          743 a         1249 c         1005 g          817 t
ORIGIN      
        1 tcccgcgaat ggggatcgaa attcaccgta tgccagagct catacctgtc caatgctttc
       61 cactgccccg ggcggatctc cgcttgcttc cggatgacta ttgaccccgg caggcctgac
      121 ccccgctgta gctttacggc aaaactcagc ccttcggtac ggatatcatc ctgatccaac
      181 tggcggacga ggtaacgttt gagccgcagg ccgtccgggc tgaggtcttc gacctgccag
      241 ttcagctcat tggcggcgtg gcgcacactc atccggtatt tgcccaggac aaattcccac
      301 aacggcagat cgtcctcttc gattcccttt attaacagcg atttccaggg cgttgaataa
      361 agctgcccaa tctttgtctc ccggcacaac aaacacaaat ctttcaggaa ggccaccggg
      421 aacatctcct cccggcggta tatgccgagc caatacttgt cgccgtaacg gttgaagcct
      481 tcataataag gcaggcggaa cggaggaagc cgccattcag gcgccgcggg cccccgggga
      541 aaagagcgtt ctatcgacac ccgatggtac agccggtctc cccaggtttc gccggcttcg
      601 gccggcctgg ctttgacaaa gccgggactc cccatctcct cctccaaaag cttgctgacg
      661 cgggcaatat ccgccgagta caccgcggtc cgccagcgga acacttcgtt tgtacccggc
      721 cgccgcagga aaagatgcca gtgatgggtc atactgctgc tgacgaagtt gagatgaccg
      781 gtgaaaaaag gcacaaacga ctgcccgccg tccaccagat tgatcttcac cgcgggccgg
      841 tgatcgaaac cctccaggat atccttgtac agaccttcgc tgacccaggc tgacttgcca
      901 aataccccct cggtgacata ggaactcaca acgtttggga acgtctcctt tccggtctca
      961 aaaaaaacac cggcggctcc cagcgattgc agcaagtccg gaccatagcg gtccaccgcc
     1021 ttgcaataca gttgttgtct ggcgccaagc tgcatttccc ctaccctcgc ctgctccgct
     1081 gctgcaacaa tggcatagag atcgccggtt gcgacgatgc ctcccggcaa attgatctta
     1141 acggtatggt tcttacgaaa catctgccaa cgcctccgcc attatcttta ttacttcagg
     1201 cctgcaactg ccacacccgg ttcccgcgcc cgtgatagaa caaagctccg ccacatcgcg
     1261 gcagccttcg gccacctttt tcttcagatt gccgctcccc acatgattgc agctgcaaac
     1321 gagccgcccc tgaacgggct cggtcctgcc gccgttccgc aatagctgga gccgcttatc
     1381 gctgagctcg atccttcggg tgatccaatc gcggaattcg ttgaattctg ccttatcccc
     1441 gatcaggatc gccccgacca tccggtcctc atggataatg cacttcttat aataccgtcg
     1501 cgccttatcg atgaagacaa tttcctcgta gtcccggctg tcgggtgatt ccgcgagccc
     1561 gatgctgcat aggtcgaagc catgtatctt gatgatattc atcggcaccg tccccgaata
     1621 atagctggca atatccccgt gcaggtacct tgcgacgatt cccgcttgtt gctccgccgc
     1681 cgccgtgaat ccaaagagac ttccctggaa ctcggcgatc tccccgatcg catagacgtc
     1741 cggatcgctg gtccgcaaat aaccatcgac gacaacgccc ctgtgatact ccaatccggc
     1801 atccttcgcg atgtcaatat tcggcaccgt accgatcgcg aagaccaggg cgtcgccctc
     1861 cagctgcctt ccgcttcgca actgcactcc ggtcaaccgc gatttccccc ggaacagctg
     1921 tacctcgtcc gagtagtaga cttcgcagcc ctggtcgacg atctcttcat gcagcaactg
     1981 gctgcccagc cggtcgagct gccggtcgag gaaacgcgag ctccgatgca cgatcgacac
     2041 cgcaattccc atctcgcgga gcgaagcggc gagctccagc ccgagcaaac ccccgcccac
     2101 gacgatcacc cggcttcctt ccgccagctc cgcccggaac cggtccgcgt ccgcacggct
     2161 tcgcaacgtg aagattccct gcagcgccgg tacctgcttc ggcatggcgg cacggctgcc
     2221 catggccagt atcagcttat catatcgcgt aacggccccg gtggaatcgg tcacggattt
     2281 tcctgcacga tcgatcttta caatactcac gccccggtgc agccggatct gaaggcgggc
     2341 ctcttcttcg tccgtcatcc ggaccagctg agcccaggtt tgtgatccgt tgatatactc
     2401 cggaagttgc acccggttat agaacggctc attttccttg ttgaagacga tgatctcatc
     2461 ctcgttgttg agcgcgcgat aactgtggat gaaaccacgc gcgcccgcgc cggcgcctac
     2521 gatgacgatc cgctcttttg tctttttgtg tttgtccacc cttaccgcgc tgtatttgta
     2581 gtccggctct ttgctgaccg ggtcgaccaa tgagttggtt agattattgg cacggtttaa
     2641 atcgctgttt agcaattttc cccagtgcat cggcaggaaa acgacgccgc gctttatcga
     2701 ttcgctaagg cgaatgggca cccgcacgtc cccacgcctt gagatgaccg ccacaatctc
     2761 accttcgcaa atgcctaact gcccggcgtc cgccggatgc atttccagga aagcctgcgg
     2821 agaatgcagt tttagtttgt tgacctttcc ggtcttcgtc atggtgtgcc actggtcgcg
     2881 gattcgacct gtcgtgagaa taaacgggta ttccgcgtcc ggccgctcgc tgcggatatc
     2941 atcgctcacc gcatgaaaga cagcccgggt tgtcggtgtc gggaaaagat ggtcgccgaa
     3001 cagccgggcg gttccctcac cggcctggcc gggcgggtac ggccattgca ccgatcgccg
     3061 tgcccggagt atttcaaagt ccagtccgct gatgtcgatg gaagtcccgg cggtcaaaag
     3121 tgcatgttcc cggaagatgg ccgccgggtc gggatagtcg aacccgggaa aacccatctt
     3181 ttcggccagt gcgcagatga tcgcggcatc gggacgcgcc tcgcccggcg ggtccaccac
     3241 cttgtccaga aggctgatcc ttcgctccga gttcgtcatg gtcccctgtt tctccgccca
     3301 gccggccgcc ggcaaaatga tatccgcgca gccgagcgtt tccggccggt tgctgatctc
     3361 ctgtacaacc acaaaccttg cattccgtaa tgcttgttcc gccttccggg catccggcag
     3421 actgaccagc gggttggtgc cgatgatcca gatggctttc atccgcccct gcgacagcgc
     3481 gtcgaacatc tccgtcgccg tgagacccgg ctccgggcaa atcggtccgc tgccccagaa
     3541 cttttgaagc tccgcgcggt gggccggatt gctgagctcc cggtgggccg gcaggaggtt
     3601 ggccaggccg cctgtctcgc gcccacccat ggcattgggc tgcccggtga gcgaaaaggg
     3661 gccgcaaccc gggcggccaa tttgaccggt caataagtgg agatcgatca gcgacaggac
     3721 cttgtccacg cctctggcgc tctggtttag tcccatcgtc caaaagctga tccaacctcg
     3781 tgcggtcgct atccactctg ccgcaagacg gatg
//
LOCUS       DATGKE010000715         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_257717, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000715 DATGKE010000000
VERSION     DATGKE010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257717"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VKU83_12135"
     CDS             <1..407
                     /locus_tag="VKU83_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94359.1"
                     /translation="HYHELNELENKFPGIRNYHITNKEVGNKVIFLRKLAPGGSTHSF
                     GIHVAKMAGMPASLLRRAGEILQQLEEKHVGSPVDESLKKIVPPKLQLSIFDAHTETF
                     EQIRRILENTDINRLTPVEALLKLQEIKNQLK"
     gene            781..1758
                     /locus_tag="VKU83_12140"
     CDS             781..1758
                     /locus_tag="VKU83_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLI94360.1"
                     /translation="MVTYSFQFENFEKWLAGFATRLEVPLHDRVVAIPSELGEGIMLA
                     RNVNPHFSYAIMNFRLAADLEMHRETGSKGFIISFNQVEAHREPGAGAGPATDKKPFF
                     RNDIFLAEASDTASLRLTAGTTVKRLLIFASHDLARQYLPADLMEKLTAFARANGAGE
                     NPLLINLTHRTMLKELFDIRDNDPLSHIKRLSNVIHLIERSLHSFLRQEQATLPRAVK
                     KTDLESMQHIERILSSRLEGFPSLESLAHEVFMSTSKLKNLFKQVYGHTLYDYYNKSR
                     LQRARDLLANGQVSIKQAGSEIGFSNLSHFAKAFRKEFGILPRDLVKGR"
     gene            complement(1765..3228)
                     /locus_tag="VKU83_12145"
     CDS             complement(1765..3228)
                     /locus_tag="VKU83_12145"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HLI94361.1"
                     /translation="MMRFVIGVDIGTTNTKAVAFTPGGEVLASAGISYPVFTDADGRY
                     ELDPDQLTEAVLTTVGVVLLKTGETPAGISFSCAFHSLLAVDERGRPMMRVMTWADLR
                     PAREARELKLAEAGRRIYEHTGTPIHAMSPLCKLLWMRNTDPLLFRNASRFIGIKEYI
                     WWRLFGKYQVDHGIASATGLFDIRRLEWYPESLALAGIGPERLSLPVPGTHIETVQCS
                     RLLELPDGVPFVIGGGDGCLANLGSGAVLPGDTALTIGTSGAIRVTAVSPQSDEKGRI
                     FNYILAANRFTCGGATNNGGNALKWLFEKLLGVLNEGDWDRMVKEAGNVPAGCQGLIF
                     LPYLQGERAPVWDAEASGVFFGVRSIHDHRHFIRACMEGICYSLYEIGASLGGTVAPT
                     DPIYASGGFTRSEIWLQMIADVFLRKVHLSGVADASAVGAAILGFQAIGLIDDTDAAA
                     GMIRIHRTYYPDEAQHVHYRRNYAIFSQLYGRLQDLK"
     gene            complement(3231..4358)
                     /locus_tag="VKU83_12150"
     CDS             complement(3231..4358)
                     /locus_tag="VKU83_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HLI94362.1"
                     /translation="MERIYFDNAATTGLDPQVLAVMMPYLTDKFGNPSSIYSYGRESR
                     LAIENSRKSVAKILNAHPAEIFFTSGGTESSNTAITAAVHDLGCVHIITSPLEHHATL
                     HTVEHLHKRGEAALSYVNVLPNGHLDMEDLERKLAASGEKTLVTLMHANNEIGNMLDI
                     HSVGELCKLYGALFHSDTVQTVGHFPFDLRNTPVHFITGSGHKFHGPKGVGLLYINEN
                     VRINPLIHGGSQERNMRAGTENVYGIVGFARALELATAAYEQDSAYIKGLKIYMMEQL
                     KRQLKGVSFNGDPLGRSLYTVLSVSFPKTERSEMLLFNLDIHHICASGGSACTSGAEH
                     GSHVIRAVNNNPNQVTVRFSFSKHNTRQEIDHVVGKLKEMI"
     gene            complement(4386..>4927)
                     /locus_tag="VKU83_12155"
     CDS             complement(4386..>4927)
                     /locus_tag="VKU83_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94363.1"
                     /translation="PGNTVSNMSSTKALKEITMAHGGRYFPSAVGEVNVVNAMKAVDA
                     VIGGEGNGGIIVPDLHYGRDALIGIGLFLSALAHHKGGVKSLRKKYPDYFISKNKIEL
                     RQGIDVPAVLEKIKKKYKNHPVNTDDGLKIEFDSDWVHLRSSNTEPIIRIYAESNFET
                     TANNIALRLMQDIKEAIAP"
BASE COUNT         1140 a         1408 c         1262 g         1117 t
ORIGIN      
        1 cccactacca cgagctcaat gaactggaga acaagtttcc ggggatccgc aattatcaca
       61 tcaccaataa ggaggtcggc aataaggtca tctttctccg caaacttgca cccggcggaa
      121 gtactcatag ctttggcatt catgtcgcca aaatggccgg aatgccggca tcgctgctga
      181 ggagggccgg ggagatccta cagcaactgg aggaaaagca tgtcggctcg ccagtcgacg
      241 aatccctgaa aaaaatcgtc ccgccgaagc tgcagctgtc gattttcgac gcccataccg
      301 aaacctttga gcagatcagg cggattctcg aaaacaccga tatcaaccgg ctcacgccgg
      361 tggaagccct ccttaaattg caggagataa agaatcagct gaaataggag gtattattcg
      421 cggtcgaaaa ccccctctct tcgtgatcgt cggcccagcg tatggcatga aattatatga
      481 atagcacagg gaggatcggc gtagtgcacg tgatggaaag gccgcagcaa ggcctttaag
      541 ggctattttt gtgaaaaata aagttaaata gctgtttcgg caattaaatg gatcgtcgca
      601 gccaaccgtg cgaggtcaat tttgcttaat tttgtgcttt tgctttgcat tggtacaaca
      661 cttcagactc atccacccct cttccctgcc gcgactgttg ccgatgagaa ttgagccgga
      721 ctttgcccgg ccgtgactgt gactttgaat tttaattaat cgcgtcaaaa acagtggact
      781 atggtaacgt attcattcca attcgaaaac tttgaaaaat ggctcgccgg tttcgccacg
      841 agactggaag tgccgttgca tgaccgggtg gtggcgatcc cttccgaact tggcgaaggg
      901 ataatgttgg cacgcaacgt taatccacac ttttcctatg cgatcatgaa tttcaggctg
      961 gccgctgatc tggaaatgca ccgggaaacc ggatcgaagg gcttcatcat ttcatttaac
     1021 caggtggaag cacataggga acccggcgcc ggcgccgggc cggcgaccga caaaaagcct
     1081 tttttccgca acgacatttt tttggccgag gccagcgata ccgccagcct gaggctgaca
     1141 gcgggaacga cggtgaaacg attgctgatt ttcgcatccc acgacctggc gcggcaatac
     1201 ctgcccgctg atttgatgga gaagctgacg gcatttgccc gggcaaacgg cgccggtgaa
     1261 aatccgcttc tgatcaatct gacgcatcgc acgatgttga aggagttgtt tgacatcagg
     1321 gataatgatc cgttgagcca tataaagagg ctcagtaatg tcattcacct gatcgagcgt
     1381 tcccttcatt ctttccttcg ccaggagcag gctacgcttc cccgggcagt caaaaagacg
     1441 gacctggaga gcatgcagca tatcgaacgc attctcagca gccgcctcga aggttttccg
     1501 tccctcgagt ccctcgcgca tgaagtattc atgagcacca gcaaattgaa aaacctcttc
     1561 aaacaggtct atgggcacac gctgtatgat tattacaaca agagccgcct tcagcgcgca
     1621 agggatctgc ttgccaacgg ccaggtgagt atcaagcaag ccggcagcga gatcggcttt
     1681 tccaatctca gccactttgc aaaagccttc aggaaggaat tcggcatcct gccccgggac
     1741 ctggtaaaag ggcgctgacc gggcctattt caggtcttgc aaccggccgt agagttgcga
     1801 aaagatggca taattccgcc ggtagtggac atgttgcgcc tcatcaggat agtaggtccg
     1861 atgtatccgg atcatccccg ccgcggcgtc ggtgtcgtcg ataagtccga tggcctggaa
     1921 cccaaggatc gccgctccca cagccgaggc gtcggctacg ccactcaggt gaacttttct
     1981 gagaaagacg tcagcgatca tttgcagcca tatctccgac cgcgtaaagc cgccgcttgc
     2041 gtagataggg tcggtgggag ctaccgtccc cccgagcgat gcgccaattt cataaaggga
     2101 gtagcaaatg ccctccatac aggccctgat aaaatgccgg tggtcgtgga tcgatctcac
     2161 tccgaagaaa acgccggacg cttccgcatc ccatacgggg gccctttccc cctgcaaata
     2221 gggcagaaat atcagcccct gacagccagc cggcacgttc cccgcttcct tcaccatccg
     2281 gtcccaatct ccttcgttca ggactcccag cagcttttca aacagccatt taagggcgtt
     2341 tccgccgttg ttcgtggcgc cgccgcaggt gaaacggttt gccgccagga tatagttgaa
     2401 gattcttcct ttttcatcgc tctgcggcga gaccgcggtc acgcggatgg cgccgctcgt
     2461 gccgatcgtc agcgccgtgt caccggggag cacggccccg ctccccaggt tggccaaaca
     2521 cccatccccg ccgccgataa cgaacggaac cccgtccggc aattccaaca atcgcgaaca
     2581 ttgaacggtc tcgatatgcg tccctgggac cggcagggac aggcgctccg ggccgattcc
     2641 ggcgagcgcc agcgactccg gataccactc caaccgccgg atgtcaaaca gcccggtcgc
     2701 cgaggcaatc ccgtgatcta cctggtactt tccgaataac cgccaccaga tgtattcctt
     2761 gataccgatg aatcgggagg cgttgcgaaa gagtagcgga tccgtgttcc gcatccacaa
     2821 gagcttgcat aacggcgaca tcgcgtgaat aggcgtaccg gtgtgctcat agatccggcg
     2881 gccagcctcc gccaacttca gttccctggc ttcccgcgcc gggcgaagat ctgcccaggt
     2941 cattacgcgc atcatcggcc tgcccctttc atctaccgcc agtagactgt gaaaagcgca
     3001 actgaagctg atgcctgccg gcgtctcgcc tgtcttcagc aggacaactc cgacagtggt
     3061 caagactgcc tccgtcagtt ggtcgggatc caattcatag cggccgtcgg catcggtaaa
     3121 tacgggatag cttatcccgg cgctcgccag cacctcgccg cccggcgtaa atgccaccgc
     3181 cttggtattt gtcgtgccga tatcaacgcc gatgacaaag cgcatcattt tcagatcatt
     3241 tctttcagct tcccgacgac atggtctatt tcctgccggg tattatgctt gctgaaagag
     3301 aaccggacag tgacctggtt gggattgttg ttaacggcgc ggataacgtg cgacccatgc
     3361 tccgccccgc tggtgcatgc actgcctccg gacgcacaga tatggtgaat gtccagattg
     3421 aacaggagca tttccgatcg ctcggtcttg ggaaacgaaa cgcttaacac cgtataaaga
     3481 ctacggccaa gcgggtcacc gttaaaactg acacctttca actgccgctt cagctgttcc
     3541 atcatgtaaa tcttcaaccc cttgatatag gcactgtcct gctcataagc agcggtggcc
     3601 aactcgagag cacgggcaaa gccaacgatg ccatacacat tttcggtgcc cgcccgcatg
     3661 tttctttcct gtgaaccacc atggattaaa ggatttatcc gcacattttc attgatatac
     3721 aacaggccaa ccccttttgg accgtgaaat ttatggccgg agccggttat gaaatgaacc
     3781 ggcgtattcc gaaggtcaaa tgggaaatgt cccactgtct gaacggtgtc agaatgaaag
     3841 agggccccat acaatttgca cagctccccc actgagtgga tatccagcat gttaccgatc
     3901 tcattgttgg catgcatcag cgtgaccagg gtcttttcgc cgctggccgc cagcttgcgt
     3961 tcgagatctt ccatatccag atgaccgttg ggcaatacgt tcacatagct cagcgcggct
     4021 tccccgcgct tgtgcaggtg ttccacggta tgcaaggtgg cgtgatgctc taacggcgag
     4081 gttataatat gcacgcatcc aaggtcgtgc acggcggcag taatcgcggt gttcgagctt
     4141 tccgtcccgc cggaagtaaa aaaaatctcc gcgggatgcg cattcaggat cttcgccacc
     4201 gatttccggg agttctctat cgccagcctt gactcccggc cataggaata gatagaggaa
     4261 ggattgccga acttgtctgt aaggtatggc atcatcaccg ccaaaacctg cggatcaaga
     4321 ccggtggtgg cggcattgtc aaaataaatt ctttccatac aagcggcaaa agtacgattt
     4381 ctccgttacg gtgcaattgc ctccttaatg tcctgcatga ggcgaagcgc tatattattt
     4441 gcggtggttt caaaattact ctccgcataa attcggatga tcggctcggt gttggaggaa
     4501 cgaagatgaa cccagtcact gtcgaattcg atcttgagtc cgtcatcggt attgaccgga
     4561 tggtttttgt atttcttttt tatcttctcc agcacggccg gcacgtctat cccctgccgg
     4621 agctcgatct tgttcttgga aataaaataa tccggatatt tcttcctaag tgacttgacc
     4681 ccgcccttat gatgtgccag cgcgctgaga aaaagcccga taccgatcaa cgcatcccgc
     4741 ccatagtgaa ggtcaggaac gataattccg ccgttccctt ccccgccgat gaccgcgtcg
     4801 accgccttca tggcgtttac gacattcacc tctcccaccg cggacggaaa ataacggcct
     4861 ccatgcgcca tggtgatctc tttcagcgcc ttcgtgctgc tcatattgct gaccgtattt
     4921 cccggcc
//
LOCUS       DATGKE010000716         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_470749, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000716 DATGKE010000000
VERSION     DATGKE010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470749"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VKU83_12160"
     CDS             complement(<1..375)
                     /locus_tag="VKU83_12160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI94364.1"
                     /translation="MDLIEPRAEAYAEKYSSGTGPLLQEIAAYTRAHHPEAHMLSGPV
                     QGKFLELISRLMRPRRILEIGTFMGYSALCLAAGLADGGKLHTIELRDEDADIAEQHF
                     RRAKESDRIILHRGNALAIIPTL"
     gene            complement(418..1956)
                     /locus_tag="VKU83_12165"
     CDS             complement(418..1956)
                     /locus_tag="VKU83_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HLI94365.1"
                     /translation="MAAPLLFSFVIGYFILLLLVAWRTSRHANNDSFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGTVGAFGDDGYKAFGYFQVIIGNWIGYLVVALVLLPLYY
                     KMNLTSIYRYLHSRFGVVAYRTGALFFILSRTVGATARLYLVINILYIFILKELGISF
                     GLTTGVILLMILLYTFEGGVKTIVYTDTLQTTFMLLGLIVCTVFILHQLGLTPGRAMA
                     AMESKGLGRIFHSDVNSKNFFPKQIIGGMAISIAMTGLDQEMMQKNISVRNLRDSKKN
                     MVAFSFIFMLVTFLFLFLGGLLYLFAVGQGGVSAAGHQLLLGGRNVIGDGLFPTLALH
                     YLPKGVAIIFILALISALFPSADGALTALTSSFCIDMLDLKNRQGLAEGRKRRIRMVV
                     HLGFAIVFLLCILIFKWVGNQSIIGVILDLAGYTYGPLLGLFAFGIFTRRVLPDSWMV
                     TALCLVTPAICWVVSERAADWFGGYQIGIELLVLNGALTFAGLSLLSRPPSRESMQWK
                     EPRE"
     gene            2021..2560
                     /locus_tag="VKU83_12170"
     CDS             2021..2560
                     /locus_tag="VKU83_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLI94366.1"
                     /translation="MQIRCVATFCGSKNGSNPLFARHTIELAKYIALLKLKLIYGGGS
                     TGLMGLMADTALAHGVDVTGVIPELLTEWEHHHKGLSQLAIVPDMHTRKRMMYERCDA
                     AIILPGGLGTLDELFELLTWNQLRIHNKKIYLLNSAGFYNHMIRHLRQLEKEGFLYDD
                     IGDRMIVCDNPVEIFNRLS"
     gene            complement(2609..3220)
                     /locus_tag="VKU83_12175"
     CDS             complement(2609..3220)
                     /locus_tag="VKU83_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94367.1"
                     /translation="MAISLTFIVAVAVGQEHMPRQVNTYTDAGNGNGFLKQNLFAGGG
                     LDLGLADYQFNIGVNPEIGYSLNRWLDAGVVVNFNYVSVSPDPYGNFNPDLSEKEFVY
                     GGGAFARAFFLPFVFATVQPEYNWTHDKQKYEQYGTQYTYNFSAASLLCGIGYAHRVV
                     GDGTFYLALMFDVLGNQYSPYNDPYGHPLPVIRAGFDFYVHKH"
BASE COUNT          866 a          981 c          891 g          719 t
ORIGIN      
        1 aagggtaggg atgatcgcta acgcatttcc gcgatgcaat attatccggt cgctctcttt
       61 tgcccggcga aagtgttgtt ccgcgatatc ggcgtcctcg tccctcaact caatggtatg
      121 cagcttgccc ccgtccgcca atcccgctgc caggcataac gcgctatacc ccataaacgt
      181 gccgatttcc aggatccgtc tcggccgcat cagcctgctg atcaattcga ggaacttccc
      241 ttgcacgggg ccgctgagca tatgagcttc gggatgatgg gcccgggtat aggctgcgat
      301 ctcctgcagc agcggtccgg tcccggagga atatttttcc gcgtaagctt ccgcccgggg
      361 ttcgatcaag tccataagaa agctttttgc aaatataact cacttacggt gaattgttta
      421 ttcgcgcggt tccttccatt gcatcgattc ccgggatggc ggcctggaca gcagtgacag
      481 acccgcgaat gtcagcgccc cgttcaatac cagcagctca atcccaatct ggtaaccccc
      541 gaaccagtcc gccgcccgct cgctcaccac ccagcagatg gccggcgtga cgaggcataa
      601 ggccgtcacc atccagctgt ccggcaggac gcgcctggtg aagatcccga acgcgaaaag
      661 gccaagcagc ggtccatagg tataccccgc caggtccagg atcaccccaa tgatggactg
      721 gtttccgacc catttgaaga tcaggatgca aagcaaaaag acaatcgcga atcccagatg
      781 cactaccatt ctgatccgcc gtttccgccc ctcggcgagc ccctgccggt tcttcagatc
      841 cagcatatcg atgcagaacg aggaggtgag cgccgttagt gcaccgtcag cactggggaa
      901 taaagcgctg atcaacgcca ggataaaaat gatcgccacc cccttgggca ggtaatgcag
      961 ggccagggtc ggaaagaggc cgtcaccgat cacattcctg ccgccgagga gcagttgatg
     1021 gccggcggcg ctgacgccgc cctggcctac ggcaaacagg tacagcaatc cgcccaggaa
     1081 aaggaagagg aaggtcacca gcatgaatat gaagctgaag gcgaccatgt tcttctttga
     1141 gtcccgcagg ttccggacgc tgatattctt ttgcatcatc tcctggtcga gacccgtcat
     1201 ggcgatgctg atcgccattc caccgatgat ttgtttggga aagaagttct tgctgtttac
     1261 atccgaatgg aagatccggc ccaaaccctt cgattccatt gcggccatcg ccctgcctgg
     1321 cgtaagcccc agttggtgca ggataaatac ggtgcaaacg atcagcccga gcaacatgaa
     1381 ggtcgtttgc agggtgtcgg tatagacgat ggttttcacg ccgccctcaa atgtgtacag
     1441 gaggatcatg agcaggatca cccctgtggt caatccaaag gagatgccca gctccttcaa
     1501 aataaaaatg tagaggatat tgatgacgag atacagcctg gccgtcgccc cgacggtgcg
     1561 cgaaaggata aagaacagcg ccccggtgcg gtacgctacc acgccgaacc ggctgtgcag
     1621 gtagcggtag atcgaggtaa gattcatttt gtagtatagg ggcaacagga caagcgccac
     1681 gaccagatac ccgatccaat tcccgatgat gacctggaaa taaccaaatg ccttgtatcc
     1741 gtcatcaccg aatgccccga cggttccggg cacggacaca aacgtcaccc ccgacaagga
     1801 agtcccgatc attccaaacg ccaccagcat ccaattcgag ttccggttcc cgataaagaa
     1861 ggagtcattg ttggcgtgcc gggaagtgcg ccaggcaacc agcagcaaca ggataaagta
     1921 gccgataacg aaactgaaca acagcggagc agccatccat gaaattagga aatacgacct
     1981 taataactga attttaattt ataagcaaag cctaccgccc atgcaaatcc gatgcgtcgc
     2041 aaccttttgc ggctcgaaga acggcagcaa tcccctgttc gcccggcaca ccattgaact
     2101 tgcgaaatac attgccctcc tgaaactgaa attgatctac ggcggcggta gtaccggtct
     2161 catgggcctc atggccgata ccgcgcttgc ccacggcgtc gacgtcacgg gggtcatccc
     2221 tgaactgttg accgaatggg aacaccacca caagggactc agccaactgg ccatcgtgcc
     2281 ggacatgcat acccgaaaaa gaatgatgta cgaacgctgc gacgcggcga ttatccttcc
     2341 gggcggcctg ggaaccctgg atgaactgtt cgaattgctg acctggaacc agttgaggat
     2401 ccataacaaa aagatatatc tcctcaactc ggccggcttc tataaccaca tgatccgcca
     2461 ccttcgccaa ctggaaaaag aaggattcct ctacgacgac atcggggacc gcatgatcgt
     2521 ttgcgataac cccgtggaga tcttcaaccg cctgtcttag ccgccgaagt tttagcgcgg
     2581 gcggctctta cccgtttttg accgtcctct agtgcttgtg cacatagaaa tcaaagcctg
     2641 cgcggataac cggtaacgga tgcccgtaag gatcgttgta tggtgaatac tggttgccca
     2701 ggacgtcgaa catcagcgca aggtagaacg ttccatctcc caccacgcgg tgggcatacc
     2761 cgataccgca caacaggctt gccgcgctga agttataggt atactgagtc ccgtactgct
     2821 catatttctg cttgtcatgt gtccagttgt actccggttg tacggttgca aaaacgaagg
     2881 gcaggaagaa tgcgcgtgca aacgctccgc cgccgtagac aaactctttc tcgctaaggt
     2941 ccggattgaa atttccgtac gggtccggcg aaaccgacac ataattgaaa ttcacgacca
     3001 cgcccgcgtc gagccaacgg ttcagcgaat agccgatttc cggattgacg ccgatattga
     3061 actggtaatc cgccagcccg agatccagac cgcctccggc gaaaagattc tgttttagaa
     3121 acccgttccc attacccgca tcggtataag tattcacctg cctgggcatg tgctcctgtc
     3181 caacggcaac ggccacgata aacgtcaggc taatggccaa aatagagatt ttttgtatca
     3241 tcatagtgca aaaatgagat aaatcggtgg tttttggaaa cgtcagggtt tgaacgaagg
     3301 acacaaggac tggcgccggt ccccgttgta ctgactgtaa aaaccctggc tctcgagagc
     3361 gaattcaaag gccccatagc cattattata gttccttaga gacgaggtcg tcgcatcata
     3421 ggtaaaggtt atcttcagat tgtgatagat gaagccg
//
LOCUS       DATGKE010000717         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1189057, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000717 DATGKE010000000
VERSION     DATGKE010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189057"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VKU83_12180"
     CDS             complement(<1..571)
                     /locus_tag="VKU83_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94368.1"
                     /translation="MKRILFVGAGKEFPNGAFRFLDSLDDARHLHVTGLFFSTIDIEA
                     MATVSHFPVISAYDRLHDQELESLHANKAVFARKCADAHLHYQVHPNEESWDIHLFAR
                     ESRFSDLAVLSGEQFYGEVDPAQPNLFLREALHVAECPVMVLPEDFLAPEHLIIGYDG
                     SRDSLHAIKQFAYLFPNYTDLPTEFIYVKE"
     gene            758..1579
                     /locus_tag="VKU83_12185"
     CDS             758..1579
                     /locus_tag="VKU83_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI94369.1"
                     /translation="MKPIVVPVNFSAASNHAARYAADLATMTGGKLHLIHILQIPPTF
                     SEYPMPESAFEALRDSASDRLKSLAGELVKRTAARVPVETDIKIGSVDSNLRKYCESV
                     KPFVIVMGAAGSPLDRFLSGSATLDALRHLPIPLLVVPEGIDFQKPGRILLACDLEDI
                     RTGMADGIGFLNELKALFGSQFEVVNVTTPGDEGKGQAEFAFSAWRARLKEIHPELHF
                     VHDSTVVHGIDEYLKEHPADWLVVFPKRHSFYELHQSRTKKIVLHSHLPIMSIHG"
     gene            1591..>2141
                     /locus_tag="VKU83_12190"
     CDS             1591..>2141
                     /locus_tag="VKU83_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLI94370.1"
                     /translation="MKPIIVPVNFSACSADAARYAVSLAEAIKADVYLLHVIQIPVAS
                     AELSMTEDLYNQVFELADKSLKEMQEELRRRNNNRVEVHTELLTGSITACVAEWCNKL
                     DPELIILGVTGPTFEKFLTGSPVNALLHLRRPILVVPEGTVFHPYRLIALACDETDIN
                     RGVPHSIPLLKALLECFHAKIDIV"
BASE COUNT          520 a          570 c          564 g          487 t
ORIGIN      
        1 cttccttaac gtaaataaat tcagtcggga gatccgtata gttgggaaag aggtaggcga
       61 actgcttgat cgcgtgaagg ctgtcccggc taccgtcata cccgataatg agatgctcag
      121 gcgccaaaaa gtcttcgggc agcaccatga ccgggcactc ggccacatga agggcctccc
      181 gcaggaagag attaggctgg gccgggtcca cttctccata aaattgttcg ccgctcagca
      241 ccgccaggtc agaaaacctg gattcccggg caaacaaatg gatatcccaa ctttcctcat
      301 tggggtgaac ctggtaatgt aagtgggcgt cagcgcattt ccgtgcaaac acagccttgt
      361 ttgcatggag cgattccagt tcctggtcat gtagccggtc ataagcggag ataacgggaa
      421 aatggctgac agtggccatt gcctctatat ctattgtgct gaagaacagg cccgttacat
      481 ggagatgccg ggcatcgtcc agggagtcga gaaaccgaaa tgccccgttg ggaaattctt
      541 ttccggcccc gacaaaaagt atgcgtttca tatgagcgtt gttttaactg attgctataa
      601 catgaagata gtcggggtcg ccagcctgaa aactgacctt tcgccggcgg acaaatgatc
      661 ttggtcattt cggctgccga cacggttcag ctttcccgct gcccggagtc atggattccc
      721 ggcagtggga taagtatttt agcgaaaaga taccgttatg aaaccgatcg ttgttccggt
      781 caatttcagt gctgcatcaa accatgcggc ccgatatgcc gccgatctcg ccacgatgac
      841 gggtggaaag ctgcatctca tccacatctt gcagatcccg ccgacctttt cagaatatcc
      901 catgccggaa agcgccttcg aggcgctcag ggactcggca tcagaccgcc tgaaatcgct
      961 cgccggcgaa ttagtcaaac gcaccgccgc acgggttcct gtcgaaaccg acataaagat
     1021 cggcagcgtg gacagcaacc tccggaaata ttgcgaaagc gtgaagccct ttgttattgt
     1081 catgggtgcc gcgggatcac cgctcgaccg gttcctctcc ggaagcgcaa cgcttgacgc
     1141 attgaggcat cttcccatac ccttgctggt tgtaccggaa ggcatcgact ttcaaaagcc
     1201 cgggcggatc ctgctggctt gcgacctcga agatatccgg accggcatgg ccgacggaat
     1261 cggttttctg aacgagttga aagcgctctt cggatcccaa ttcgaggtgg tcaacgtgac
     1321 gactccaggc gatgaaggca agggtcaggc agaatttgca ttcagtgcgt ggcgggcccg
     1381 cttaaaagag attcatcctg aattgcattt cgtccacgac agcaccgtcg tccatggtat
     1441 tgatgagtat cttaaagaac accctgcgga ctggctggta gtttttccta aaaggcactc
     1501 cttttacgag ttgcaccaaa gccggacaaa aaagatcgtg ctgcatagcc atttgcccat
     1561 tatgagcatt catggataaa tcccaaagtc atgaaaccaa tcatcgtccc ggttaacttt
     1621 tccgcctgct cggccgatgc cgcaagatac gctgtcagcc ttgccgaagc gataaaggca
     1681 gacgtatact tattacacgt cattcaaata ccggtagcaa gcgctgaact gtctatgacc
     1741 gaggacctgt acaaccaggt ctttgagctg gctgacaaga gcctgaaaga aatgcaggaa
     1801 gagttgcgca ggcggaacaa caaccgggtt gaggttcata ctgagttact taccggcagt
     1861 ataaccgcct gcgtggcaga atggtgcaac aagctggatc cggaactgat catcctcggc
     1921 gtaacgggtc ctactttcga gaaatttctg accggcagtc cggtgaatgc tttattgcac
     1981 ctgcgccggc ccatactggt cgttccggaa ggtaccgtct ttcatcccta ccggcttatt
     2041 gccctggcct gtgacgaaac ggatatcaac cggggagttc cgcattcgat cccgctgcta
     2101 aaagcgctcc tggagtgttt ccatgcaaag atcgatatcg t
//
LOCUS       DATGKE010000718         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_89637, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000718 DATGKE010000000
VERSION     DATGKE010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89637"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="rpoN"
                     /locus_tag="VKU83_12195"
     CDS             <1..836
                     /gene="rpoN"
                     /locus_tag="VKU83_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLI94371.1"
                     /translation="NSVDTAIDVLSNYFDEFTKKHYDKIQRGLNLTDDELKEVINQII
                     KLNPKPGGDIGEVNKAESYVIPDFFISNNAGKLELSLNSKNAPDLRISEGYKEMLKEY
                     DRGSKKDKRQKEAVLFIKQKIDAAKWFIDAIKQRQQTLFNTMHTIMEYQHEFFLTGDE
                     TTLRPMILKDIAERTALDISTVSRVANSKFVQTEFGTYRLKFFFSESLSTDSGEEVST
                     REVKKILSDLIEGESKKKPLSDEKLTELLQEKGYNIARRTVAKYREQLNIPVARLRKE
                     L"
     gene            847..1476
                     /locus_tag="VKU83_12200"
     CDS             847..1476
                     /locus_tag="VKU83_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94372.1"
                     /translation="MTGVTRFWANFFSYVFHPLFISSYVMAFLIFCHPMAFAEFDHKT
                     RVLRFVTILTCNVIFPLFSVFVMWKLDLYVKSMYLRTEKERIVPYLIAMIFYWWTWYL
                     FKNLAEVPVVARHFLAGSFLALVGAFLCNIYYKISIHAVAIGSALMFFFLFSFTDEYA
                     SGLYLSIATLIAGLVASSRLLVGSHTSFDVWSGLFVGMLAQLIGWYGPL"
     gene            complement(1555..2289)
                     /locus_tag="VKU83_12205"
     CDS             complement(1555..2289)
                     /locus_tag="VKU83_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HLI94373.1"
                     /translation="MGRRYLKPLVWCICIAIFIPVNALKAGGNPVRSHSSMRAAKAAA
                     LAEEVSVLYDEFNLKELGLTKKAFYYALKGYNYLLEHHWLNNPNILSICDLSQSSRNK
                     RLYVLDLENKQVLVNTYVAHGRASGAEFAHYFSNSSSSHKSSLGFYVTEDTYYGSNGL
                     SLRIKGLERGFNDRALGRNIVVHGSEYVGPDFLQMNRFCGRSYGCPAVPADESENIID
                     LIKEGSCLFIYYPKKIYLTRSRILNS"
     gene            complement(2458..>3553)
                     /locus_tag="VKU83_12210"
     CDS             complement(2458..>3553)
                     /locus_tag="VKU83_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLI94374.1"
                     /translation="RRIGEKKPQAAAHAAGQSILLSIIFTLAISLSGALAASSILRLM
                     GAGPSTVAAGTAYTRIEFGGCIVIVLLFLINGIFRGAGNAAMAMWSLWIANGCNILLC
                     PLLIHFFGLPGAAIATTAGRGAGVCFQLYHLLRGSPMLPKLPRIVPDRKVMAGLLKIA
                     WSGTVQFFINSASWIVMTRLIAQFQDVAIAGYLVSIRILIFFLLPAWGMSNAAATLVG
                     QSLGAGLPGRAVRSVRTTAIYNGVFMAAVTLISFSCAVPIVGFLNKDPAVEVIAVRAL
                     RIISLGYIFYGVGMVLTNAFNGAGDTLTPMIINLFCFWLFQIPLGWLLADYFHYGPTG
                     VFIAILLTESTLTVVSLVVFSRGKWKKVKI"
BASE COUNT          884 a          985 c          870 g          814 t
ORIGIN      
        1 ataattctgt cgatacggcc atcgatgtcc tatcgaacta tttcgacgaa ttcaccaaga
       61 agcattacga caagatccag cgcggcctca acctcaccga cgacgagctg aaagaggtga
      121 tcaaccagat catcaagctt aacccgaagc ccggcggcga tatcggggaa gtcaacaaag
      181 ccgaaagcta tgtcatccct gatttcttca tctctaataa cgccggaaag ctggaactgt
      241 cccttaattc aaaaaacgcc cccgacctgc ggatcagcga gggatacaag gagatgctga
      301 aagagtatga ccgcggtagc aagaaggaca agcggcagaa ggaggccgtg ctgttcatca
      361 aacaaaaaat tgacgccgcc aaatggttca tagacgcgat caagcaacgt cagcagaccc
      421 tctttaacac catgcacacg atcatggaat accagcacga attcttcctg accggcgatg
      481 aaactaccct caggcccatg atcctgaaag atatcgccga acggacggct ctggatatct
      541 ccacggtcag ccgcgtcgcc aacagcaagt tcgtccagac cgaattcggg acctaccggc
      601 tgaagttctt tttcagcgag tccctcagca cggacagcgg cgaagaagtt tcgacccgcg
      661 aggttaaaaa gatcctcagc gacctcatcg agggtgagag caaaaagaaa cccctcagcg
      721 acgagaagct cacggagctg ctgcaggaaa aaggatataa tatcgcccgc cggaccgtgg
      781 cgaaataccg ggaacaattg aatatccctg tcgccaggct gcgcaaggaa ctataaaacc
      841 tcagatatga ctggtgtaac gcgattttgg gcgaactttt tctcctacgt ttttcatccc
      901 ctttttatca gcagctatgt catggccttt ctgatcttct gccatcccat ggctttcgcg
      961 gaattcgatc ataagaccag ggtactccgg tttgtcacga tcctgacctg caacgtcatc
     1021 tttccgcttt tttcggtctt tgtgatgtgg aagctggacc tgtacgtgaa atccatgtac
     1081 ctgaggaccg aaaaggaaag gatcgtcccc tacctgatcg ccatgatctt ctattggtgg
     1141 acctggtacc tgtttaagaa tctggcagaa gtgccggtgg tggccaggca ttttctggcc
     1201 ggttcctttt tggcgctggt cggcgccttc ctctgcaaca tctattataa gatcagcatt
     1261 cacgccgtcg ccattggcag cgccctgatg ttcttcttcc ttttttcatt taccgacgag
     1321 tatgcctccg ggctctacct gtccatcgcg acacttatcg cgggcctcgt tgcaagctcc
     1381 cgcctgctgg tgggatctca cacgagtttc gatgtctggt cgggattgtt tgtcggcatg
     1441 cttgcccagt tgatcggctg gtatggtccg ctgtaaaaaa gaaaagccca atcatagggt
     1501 acgaacctaa attgagcttt ttcaccttca aaccatctga agtgtgcgtc catgctagga
     1561 gttcagtatc ctggaccgcg tcagatatat ctttttaggg taataaataa acaaacacga
     1621 accttccttg atcaggtcga tgatattctc cgattcatcc gcggggaccg ccggacagcc
     1681 gtagctcctg ccgcagaacc ggttcatttg cagaaaatcc ggcccaacat attccgaacc
     1741 atggaccact atattgcgac ccagggccct gtcgttgaat cctctttcca atcccttgat
     1801 cctcagggat aggccgttgc ttccataata cgtgtcctcg gtgacgtaga agcccaggct
     1861 gcttttatgg gacgatgaac tgttcgagaa ataatgtgca aattcggctc ccgacgcccg
     1921 gccatgggcg acatacgtat tgaccaatac ctgcttattc tccaggtcaa gcacatacaa
     1981 acgcttgttg cgggaggact ggctcaggtc gcagatcgat aaaatattgg gattgttcag
     2041 ccaatgatgt tcgagtagat agttgtatcc cttcaacgca taataaaaag ccttcttggt
     2101 aaggcccagt tctttcagat tgaactcatc gtacagcacg cttacctctt cggcaagcgc
     2161 cgcggccttc gccgccctca tgctgctatg gctcctaacg ggattgcccc cggccttcag
     2221 agcgttgacc gggataaata ttgcgatgca aatgcaccat accagtggtt tcagatacct
     2281 gcgacccatg cttcaaagtt ttattctgcg gctttcaggg cttcctgttt gttcgcccgc
     2341 ctttattaaa aacggcgggg aatctaaaat attatctatt ttctgattgt gcaatagtct
     2401 tttatgaaaa gtttcattat tttttgagca ataatttgac cgtttttgag tatcccccta
     2461 aatctttacc tttttccact taccccggct aaaaacgacc aaactgacca ccgtgagggt
     2521 ggattccgtc agcagtattg ctataaatac gccggtcggt ccgtaatgaa aatagtcggc
     2581 aagcagccag ccgaggggaa tctggaagag ccaaaagcaa aacaggttga taatcatcgg
     2641 agtcagtgtg tcgccagccc cattgaaggc gttcgtcagc accatcccaa caccataaaa
     2701 gatgtagccg agactaatta tccgcaaagc ccgtacagct ataacttcta ctgccgggtc
     2761 tttgttcaaa aagccgacga tcgggaccgc gcaagagaaa gatatcagcg tgacggcggc
     2821 cataaaaact ccattgtata tcgccgtcgt ccttaccgat ctcaccgccc gcccgggcag
     2881 gccggcccca aggctctgac cgacgagcgt cgccgcggca ttgctcatgc cccaggcggg
     2941 caagagaaaa aagatcagga tgcggatcga caccaggtat cccgcgatcg ccacgtcctg
     3001 gaattgcgcg atcagccggg tcatcacaat ccagctggct gaattaataa aaaactgcac
     3061 ggtaccgctc caggcgatct tcagtaaccc ggccattact tttctgtccg gcactatccg
     3121 cggtaatttt ggcagcatgg ggctgccccg caacaaatga tacagttgga aacagacacc
     3181 cgcgccccgg cccgcagtgg tggcgatcgc ggctcccggc agcccgaaaa aatggatgag
     3241 cagcgggcag agcaggatat tgcagccgtt tgcgatccac aaggaccaca tcgccatcgc
     3301 cgcgttgccc gcgccgcgaa agatgccgtt gatcaggaag agcagcacga tgacaatgca
     3361 gccgccgaat tcaatcctcg tgtaggccgt tcctgcggca accgtcgacg gacccgcgcc
     3421 catgagccgg aggatggacg aggcggcaag ggccccggac aagctgatcg cgagcgtaaa
     3481 gatgatcgac aaaagaatgg actgccccgc cgcatgtgcg gctgcctgcg gttttttctc
     3541 accgattcgc ctc
//
LOCUS       DATGKE010000719         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1432696, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000719 DATGKE010000000
VERSION     DATGKE010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432696"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VKU83_12215"
     CDS             complement(<1..382)
                     /locus_tag="VKU83_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase family protein"
                     /protein_id="HLI94375.1"
                     /translation="MRQQIAAANWKMNLTYQQGEELLDIILNENIVLADHQRAVFAVP
                     FPYLVMAHHRLAGKKNYFVAAQNCSDKKSGAYTGEVSAEMLRSIDVRYCVLGHSERRE
                     YFSEQNQTLAGKAAICLENGITPIF"
     gene            complement(398..1453)
                     /locus_tag="VKU83_12220"
     CDS             complement(398..1453)
                     /locus_tag="VKU83_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HLI94376.1"
                     /translation="MLLLEDNSCTNDLLPFSAIRSVLDIRMGILTLREKWALLLGVKG
                     FASVGMTKGPALPANLLPTRALAAVVREELERGPLSQDAVGRLMVHGRLVRYPWHIFQ
                     HNSEALIEDFSLVTAGRKSLPIPPSVQTSGDMGIFIEPGARLSHCILNAETGPIYIGP
                     GAEIMEGALIRGPFACCEGAVVKMGAKIYGATTLGPFCTAGGELKNAVLLGYSNKGHD
                     GYLGDAVIGEWCNLGAGTSASNVKNNAGIVSVFHPASGTWLPAGQKCGLLMGDYSRSA
                     INTAFHTGTMTGICCNIFGAGSPPKYIPDFSWGQDGQTRYEWDKALRDIRNWKKMKAG
                     DMKDEEIKTLRHIFEQF"
     gene            complement(1553..1807)
                     /locus_tag="VKU83_12225"
     CDS             complement(1553..1807)
                     /locus_tag="VKU83_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HLI94377.1"
                     /translation="MKKGIHPSAYRFVVFKDMSNGVTFLSKSSAQSRETIKYDDGNEY
                     PLIKLEISNTSHPFFTGKNVLVDTTGRIDKFKKRYEKKAK"
     gene            complement(1914..2405)
                     /locus_tag="VKU83_12230"
     CDS             complement(1914..2405)
                     /locus_tag="VKU83_12230"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HLI94378.1"
                     /translation="MPSFDIVSKVDQQVLDNAVNVTRKEITNRFDFKDSPVKIDLNKK
                     DMKIDLEAESEMKLKQIVDVLISRSMKQGLDPLAFDFGKEAYQSGRVTRQEVAVRNGL
                     KQEDAKKVTKLIKDSGLKVQSQIMDDLIRVTGKKIDDLQAVIKLCSGANLGIALQYVN
                     MRS"
     gene            complement(2545..>5038)
                     /locus_tag="VKU83_12235"
     CDS             complement(2545..>5038)
                     /locus_tag="VKU83_12235"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI94379.1"
                     /translation="TAVLAILIATVGRTQPTPLRATDLAAESAGRISGNIIDDRGQAM
                     ESVTVSLLQAADSGRVRETVTNKAGHFGFSDLPVGKYVIAASFVGYKTVYSQPVDLVV
                     GQGRVVPPLVLVSTSTSLTAVAVVGRRPPIEQKPDRTIINVDASPANSGSTAMDVLEK
                     APGVTLDKDDNISLKGKQGVMVMIDGKPTYLSASQLAEYLRSLPASAIDQIELMTNPS
                     AKYDASGNSGIINIKTKKNKLKGFNGNLNLTQTQGVYSKPSGSLNLNYRTGQLNFFVN
                     GGYSHWEGFQVLQIQRKYLDNDPAQTVNSIFQQTTVMHFINPEGNLKFGLDYFVTPKT
                     TIGFVVSGFANTETDKSGSNIQLLSPSYAIDSLVYSPNTNHTSWKNGSANLNFKHSYD
                     SAGRELTADIDYVRYSSVSDQSFNSQTFSPDKDLLGASVLTGHLPSTINIYTAKTDYT
                     RPLSGGYKLETGAKLSYVDTRNTADYYDVLNGKPNVDTTKTNAFLYRENVNALYVNVT
                     KQYGKKWSAQAGLRAENTNYSGHQLGNGLTVINNDSSFSRSYINLFPTAYVSYQANEK
                     NQFTLNYGRRIDRPAYQDLNPFLFFLDQYTYQAGNPYLQPQYTDNVELSYGYKHWLMT
                     TLNYSQTKNFFTETFEQSGQATIVRHGNIGNRQNAGIAVSAQVPVTKWWMAILYSNVN
                     YDKFTGMLYGQNLDVSATTLLLNVNNQFTFPHGWSGELSGFYRTKGVEGQVIIDPMGQ
                     GTVAIAKKILKDKASLKLAVRDFLYTNQVHGYINFQETDATFQNRRDSRMVALTFSWN
                     FGKPMKGISNGGRRNGGAGEEESRVKAGGNGN"
BASE COUNT         1062 a         1375 c         1302 g         1299 t
ORIGIN      
        1 aaaatatggg tgtgatgccg ttttccaggc aaatcgccgc cttgccggcc agggtttggt
       61 tttgttccga aaaatactcc cgtcgttcgc tatggcccag cacacagtat ctgacatcta
      121 tcgaccgcaa catttcggca gaaacctctc ccgtataggc cccggatttt ttatcagagc
      181 aattttgagc cgccacgaaa taattctttt tgcccgccag gcggtgatgc gccatgacga
      241 ggtacggaaa cgggacagcg aataccgccc gctggtggtc cgcgagaacg atgttctcat
      301 tgaggatgat atccaacaat tcctctccct gttggtacgt caggttcatc ttccaattgg
      361 cggctgcaat ttgttgtctc attgatggtt tattgattta gaattgttca aaaatatgcc
      421 gtagcgtctt tatttcttcg tccttcatat ctccggcttt catcttcttc cagttgcgga
      481 tgtcccggag cgccttgtcc cattcatacc tcgtttggcc gtcctgcccc caactgaagt
      541 cggggatata tttaggagga ctgccggcgc cgaagatgtt acagcaaatg cccgtcatcg
      601 tgcccgtatg gaaggcggta ttgatcgccg agcgggaata gtctcccatc agcagcccgc
      661 atttttggcc cgccggaagc caggttcccg acgccggatg aaagacgctg acgataccag
      721 cattattctt gacgtttgaa gcggaggtgc ccgcacccag attacaccat tctccgatca
      781 ccgcgtctcc gaggtaaccg tcgtgtcctt tgttcgaata accaagaagc acggcattct
      841 tcagctcacc gcctgctgta caaaacggtc cgagggtggt cgccccgtag atcttcgcac
      901 ccatcttgac gacggcgcct tcacagcagg cgaaaggccc gcggatcaaa gcaccttcca
      961 taatttcggc gccgggaccg atatagatcg gaccggtttc ggcgttcaat atgcaatgcg
     1021 acagccgcgc gccgggttca ataaatatgc ccatgtcgcc gctcgtttgg acggaagggg
     1081 ggatgggcag gcttttcctg ccagcggtaa cgaggctaaa gtcttctatg agtgcttccg
     1141 aattgtgttg gaaaatatgc cacggatacc ggaccagcct tccatgcacc atcagccggc
     1201 cgaccgcatc ctgtgacaaa ggtccgcgct ccagctcctc ccggaccacc gccgccaggg
     1261 cacgcgtagg aaggaggttg gccggtagcg ccggcccctt cgtcataccc acggaggcga
     1321 aacccttcac tcccagcaat aatgcccatt tctcccgcag cgtcaggatt cccatccgga
     1381 tatccaaaac ggaacgaatg gccgaaaagg gaagcaggtc gttggtgcag gaattgtcct
     1441 ccaggagaag catggcacca aatctaccaa tgtccggcaa acaccgcgcg tttcgttaaa
     1501 aaaaattccc ttccaggccg gaagggaaat gatttgcagg cgctccgatc gcttatttgg
     1561 ccttcttctc gtaacgcttc ttaaacttat cgatcctacc cgtcgtgtcc accagcacat
     1621 tttttccggt gaagaacgga tgagaagtgt tggagatttc caatttgatc aggggatatt
     1681 cgttgccgtc gtcatacttg atcgtttcgc gggactgggc agacgatttg ctcagaaatg
     1741 taaccccatt gctcatatcc ttaaagacga caaagcgata ggcgctcgga tggatacctt
     1801 ttttcataac ttattctgtt tgaaggctgt acggatttgg ccgtacaatt ttttacttag
     1861 gtttgcaaag gtacgcattt gtgcctgccc gcacaaataa aattcactta tcgctaactc
     1921 cgcatgttca catactgcag cgcgattccg agattggcgc cgctgcatag cttgatgacg
     1981 gcctgcagat catcgatctt cttccccgtt acccggatga ggtcgtccat gatctgcgat
     2041 tgcactttca gaccggaatc cttgatcagt tttgtcacct tctttgcatc ctcctgcttt
     2101 aacccgttgc ggacggccac ttcctgccgg gtaaccctgc cgctctgata ggcctctttt
     2161 ccgaaatcaa acgccagtgg atccagaccc tgcttcatcg accggctgat cagcacgtcg
     2221 actatctgtt tcagcttcat ttcgctttcg gcctccaggt cgatcttcat gtcctttttg
     2281 ttgaggtcta tcttaaccgg cgaatccttg aagtcgaacc ggttcgtgat ttctttcctg
     2341 gtaacattga ccgcgttatc cagcacctgc tggtctacct tgctgacgat atcgaaggat
     2401 ggcatagtag gctgttttgt gcctccggct tccatggccc gaaataccct tcggcgttaa
     2461 accgatcatg ccgggccttc ggccaatggc caggggccat ttaagccaaa tgtacagaaa
     2521 tgcgcggact tgccggcgag cccctcaatt cccgttccca ccggctttta cccggctctc
     2581 ctcctcccct gccccgccgt tccgccgccc tccgtttgat atacccttca tcggcttgcc
     2641 gaaattccag ctaaatgtca acgctaccat ccttgaatcc cgccggtttt ggaaagttgc
     2701 gtccgtctcc tgaaaattaa tatacccgtg gacctggttg gtatacagga agtcgcgtac
     2761 tgccagcttc aaacttgctt tatccttcag gatctttttg gcgatcgcca ccgtcccctg
     2821 gcccatgggg tcgatgatga cctgaccctc cacccctttc gtccggtaga agccggagag
     2881 ttcccctgac cagccgtgag ggaaggtaaa ttggttgttg acattcagca acagggtagt
     2941 cgctgaaaca tccaggttct ggccatataa catgcccgta aacttatcgt aattcacgtt
     3001 actgtacagg atggccatcc accatttggt gaccggcacc tgcgcgctta ccgcgatgcc
     3061 ggcattttgg cggttcccga tattcccgtg gcggacgatg gtggcctggc cggattgttc
     3121 gaaagtctcg gtaaagaaat tctttgtttg gctatagttc agcgttgtca tcagccagtg
     3181 cttgtatcca taggacagct ccacattatc ggtatactgc ggctgcagat atggattccc
     3241 tgcctggtag gtgtactggt ccaggaagaa gaggaatggg ttgagatcct ggtaggcggg
     3301 acgatcgatc cggcggccgt aattcaacgt aaattgattc ttttcgtttg cctgatagga
     3361 gacgtaagcc gtcgggaaca ggttgatata actccgcgaa aaggacgaat cgttgttgat
     3421 gaccgttaaa ccgttcccca gctggtgccc gctataattg gtgttctcgg ccctcaaacc
     3481 ggcctgcgcg gaccactttt tcccatattg tttggtaacg tttacatata gggcattgac
     3541 attttcccga tagagaaagg cgtttgtttt cgttgtgtcg acgttcggtt tgccgttcag
     3601 cacgtcgtag tagtccgccg tgttgcgggt atcgacatag ctcagctttg caccggtctc
     3661 cagtttgtag ccgccggaga gcggccgggt ataatctgtt ttcgccgtat aaatattgat
     3721 tgtgctcggc agatgacccg tcaggacaga cgcacccagc aagtccttat ctggtgagaa
     3781 ggtctggctg ttgaaagact gatcgcttac cgacgaatac cggacataat cgatatccgc
     3841 cgtcagctcc cggcccgcgg aatcatagct gtgtttgaaa ttcaggtttg ccgagccatt
     3901 cttccaggag gtatggttgg tgttaggaga atagaccagc gaatcgatgg cgtagcttgg
     3961 gctcagcagt tggatattgc ttcccgactt atctgtctcg gtgttcgcga atccgctcac
     4021 tacaaaccca atggtagttt tgggcgtgac aaaatagtcc agcccaaatt tcaggttgcc
     4081 ctccggattg ataaaatgca tgacggtcgt ctgttgaaag atcgaattga cggtttgggc
     4141 cggatcatta tccaggtatt tccgctggat ctgcaagacc tggaagcctt cccagtgcga
     4201 gtagccgccg ttgacgaaaa aattgagctg accggtccgg taattgagat tgaggctacc
     4261 gcttggttta gaatagactc cctgggtctg ggtcaggttg agattgccgt taaagccttt
     4321 taatttattc tttttcgttt tgatattgat aatgcccgag ttgccggacg catcatattt
     4381 ggcggagggg ttggtcatca gttcgatctg gtcgattgcc gacgccggaa ggctgcggag
     4441 atattcggcc agctgggaag cagacaaata ggtgggtttg ccatcaatca tcaccatgac
     4501 tccctgtttc ccctttaggc tgatattgtc atccttgtcc agcgtcacgc ccggcgcctt
     4561 ttccaggaca tccatggcgg tgctgcctga gttcgcaggc gaagcgtcga cgttgataat
     4621 ggtccgatcg ggtttttgtt caatgggcgg acgcctgccg accacggcaa ctgcggtgag
     4681 actggtggaa gtgctcacca ggacaagcgg cggcacgacc cgaccctgtc ccactaccag
     4741 gtcgacgggc tgagagtata ccgttttata gcctacaaac gaggctgcga tcacgtattt
     4801 cccgactggg aggtcggaga acccaaaatg gccggcctta ttggtgacgg tctcccttac
     4861 ccggcctgag tcggccgcct gcaaaagaga aacggtgacg gactccatgg cctgtcccct
     4921 atcgtcgatg atattcccgg agatcctccc cgcagattcg gctgcgagat cggttgccct
     4981 gagcggcgtc ggttgcgtcc ggccgacggt cgcgattaag attgctaaaa ctgcagtc
//
LOCUS       DATGKE010000720         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_259216, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000720 DATGKE010000000
VERSION     DATGKE010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259216"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VKU83_12240"
     CDS             <1..1046
                     /locus_tag="VKU83_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLI94380.1"
                     /translation="TPLQLAGGENNAYTTNDFTNYYIQLPAENIETAFWLESDRMLSL
                     AFSEKSLEVQRKVVMEEFKEHYINKPYGDVWFKMRELVYREHPYRWMTIGSDLSHIEQ
                     ARLEDVRNFFFKYYRPVNAILVVAGKTNVVQVRALAEKWFGDIPSGERYVRNLPKEPE
                     QKEARRLSVKAEVPLDALYKCYPMSARTDPGYYIADLITEVLGGGASSRLHQSLIKEK
                     KLFSQIECYHTGSVDPGLVVIEGKLIKGVSMEAADAAVEAELDKIKAGPISDKELTKV
                     KNRTESAIVFEDMSVMNRANSLAIYELLGDAGMMNTELDKYQAVTAAGILETSRQIFD
                     PLNSNTLYYYSEK"
     gene            1053..2357
                     /locus_tag="VKU83_12245"
     CDS             1053..2357
                     /locus_tag="VKU83_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLI94381.1"
                     /translation="MHVQERYKLDRSIAPPIKDAKEFELSLPPYKKYTLTNGVEVYTI
                     DLGNEEAMMINWIFDAGNWWEPKKGIAAAVSTLLKNGTRNRSAFDISEHFEYYGAYLN
                     RAAYHETAEISLHCLNRYVRELLPVVAEIVSDSTYPQEELDIYKRNSQQRLQVNLKKG
                     DFVASRLIDAYLYGEAHPYGRYSRLEDYPALQREELTAFHDRYYRQGTCRILAAGKLP
                     ADLIGLLESSFGSLPLHAPGKEPRPAFPILPATTKNYRITNDPNGVQGAIRIARNFPN
                     RHHPDFQKMQVLNNVFGGYFGSRLMTNIREDKGYTYGIHSYLVNNIQESALLISTEAG
                     KEVSEPAIREVYKEMDLLRTDLIDEEELQQARNFTIGVILGDLDGPFHVAGRWKNILL
                     NGLDGDYFKEGVRIIKTITPEELRELAIKYLDPAAFFELVVV"
     gene            2476..2826
                     /locus_tag="VKU83_12250"
     CDS             2476..2826
                     /locus_tag="VKU83_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLI94382.1"
                     /translation="MGFLLRIVITAVVAFALSYILPGIHIDTFWTAIKLSLVLAVLNF
                     LLKPILIILTLPITIVTFGFFLFIINALIVWFAGELVRGFHVDSFMWALLFSLLLSLL
                     TSILYKERDKEDAS"
     gene            3002..>3591
                     /locus_tag="VKU83_12255"
     CDS             3002..>3591
                     /locus_tag="VKU83_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HLI94383.1"
                     /translation="MHFERKNASNEELIRLYKSLLLPRMIEEKMLVLLRQGRISKWFS
                     GIGQEAIAVGATLAMDPEEWILPLHRNLGVFTSRGMPLSKLFLQWQGDADGYSKGRER
                     SFHFGSREHAICGMISHLGPQLGVADGIALAYKLQKKKQATLVFSGEGGTSEGDFHEA
                     LNVASVWDLPVIFLIENNGYALSTPVSEQYRCDSLV"
BASE COUNT          878 a          936 c         1010 g          767 t
ORIGIN      
        1 agacgccttt gcagctggcg ggcggagaga acaatgccta taccaccaac gattttacca
       61 attattatat tcagctgccg gccgagaata tcgagaccgc tttctggctg gagagcgacc
      121 gcatgctctc gctggctttc agcgagaaaa gcctcgaagt gcagcgcaag gtggtgatgg
      181 aggagttcaa agaacattat atcaacaagc cctatggcga tgtctggttc aagatgcggg
      241 agttggtcta ccgggagcat ccctatcgct ggatgaccat cggcagcgat ctcagccata
      301 tcgagcaagc cagacttgag gatgtccgga acttcttctt caaatattat cggccggtaa
      361 atgccatcct ggtggtggcg ggcaagacca atgtcgtgca ggtccgcgcg ttggcggaga
      421 aatggttcgg cgatatacct tcgggtgagc ggtacgtccg taacctgccc aaagagcccg
      481 aacagaagga ggcccgcagg ctctctgtga aggccgaggt cccgctggat gcgctgtata
      541 aatgttaccc catgtcggcg cggacggatc ccggttatta tatagccgat ctcatcaccg
      601 aagtgctggg cggcggagct tcttcgcggc tgcaccagtc gctgataaag gagaagaaat
      661 tgttcagcca gattgagtgc tatcataccg gttcagtgga tcccgggctg gtggtcatcg
      721 agggaaagct gatcaaagga gtgagtatgg aagccgcgga cgcggcagtc gaagcggaac
      781 tggataagat aaaggccgga ccgatctccg acaaggagct cactaaggtc aagaaccgca
      841 ccgagtcggc catcgtcttc gaagatatga gcgttatgaa ccgcgccaac agcctcgcca
      901 tctatgagct cctgggagac gcggggatga tgaataccga gctggacaag taccaggcgg
      961 taacggccgc cggcatcctg gaaaccagcc ggcagatctt tgatcccctc aatagcaata
     1021 ccttatatta ttacagcgaa aaataagccg aaatgcacgt tcaggaaaga tacaaattgg
     1081 atagaagtat tgcgccgccg ataaaggatg ccaaggagtt cgagctttcc ctgccgcctt
     1141 acaagaagta tacacttacc aacggcgtgg aggtctacac gatagacctg ggaaacgaag
     1201 aggcgatgat gatcaactgg atcttcgacg ccgggaattg gtgggaaccc aaaaaaggga
     1261 tagccgccgc cgtgagcact ctgctcaaga atggaacgcg aaaccggagc gcattcgata
     1321 tcagcgaaca ctttgaatat tacggtgcct atctgaaccg cgccgcctac catgagacgg
     1381 ccgagatcag cctgcattgt ctcaacagat acgttcggga gctgctgccg gtggtagccg
     1441 agatcgtgag cgattcgacc tatccgcagg aagagctgga catctataaa cggaacagcc
     1501 agcagcggct gcaggtcaat cttaagaagg gcgattttgt ggcgagccgg ctcatcgatg
     1561 cctacctgta tggcgaagcg catccgtatg gaagatattc ccggctggaa gactatccgg
     1621 cattgcaacg ggaagaactg accgcctttc acgatcgcta ttaccgtcag gggacctgcc
     1681 gtatcctggc cgcaggaaag ctgcccgccg atctgatcgg cttgctggaa tcatcgttcg
     1741 gcagcctgcc gcttcatgca ccggggaaag agccgcggcc cgcattcccc atcctccccg
     1801 caacaacaaa aaattatcgg ataacgaatg atcccaacgg cgtccaaggc gccatccgga
     1861 tcgcccggaa cttccccaac cggcatcatc cggactttca gaaaatgcag gtgctgaata
     1921 atgtattcgg cggctatttt ggttcccgtc tcatgaccaa tatccgggaa gacaagggct
     1981 atacctatgg catccacagc tatctggtca acaatatcca ggagagcgcg ctgctgatct
     2041 ccaccgaggc cggtaaggag gtctccgaac cggccatccg ggaagtgtat aaggaaatgg
     2101 acctgttgcg tacggacctc atcgatgaag aggagctgca acaagcccgg aattttacga
     2161 tcggcgtcat cctgggggac ctcgacggac cgttccatgt cgccggccgc tggaagaaca
     2221 tcctgttgaa cgggttggat ggcgactatt ttaaggaagg ggtccggatc atcaagacca
     2281 taacaccgga agagctccgg gagctggcga taaaatacct ggaccctgcc gctttcttcg
     2341 agttggtggt agtttgagcg gccaagggcc gctcggccga aggcccatca tttgccgtta
     2401 tgagaccgaa tccaatattt ggctgtagga ttcggtcgaa taaacataat gccgtaactt
     2461 gaccaaaaga ttactatggg cttcctgctc cgtattgtga tcacggcggt ggttgctttt
     2521 gcattgtctt atattttacc gggtatccat atcgacactt tctggacggc gatcaagcta
     2581 tcgctggtgc tggcggtgct taactttttg ctcaagccta tactgatcat ccttacctta
     2641 ccgatcacga tcgttacctt cgggttcttc ctttttataa ttaatgccct catagtctgg
     2701 tttgccgggg aattggtgag gggtttccat gtcgacagct ttatgtgggc gctgcttttc
     2761 agcctgctgc tctccctgtt gacctcgata ctgtataagg aaagggataa agaagacgcg
     2821 tcttaagcgc cacaacgtct aagcgcctaa gatgccggaa gcatcttgtc cacgggcgaa
     2881 gcccgcgacc gaggcccgct ccaccggagc gggccgaagt tcaacggacg ttagccgttt
     2941 gcgggaaatc cgctatattt ccttgggcgg aattcccgca aataaaaata ttcgatgagc
     3001 catgcacttt gaaagaaaaa acgctagcaa cgaagagctg atccgtctct acaaaagcct
     3061 attgcttccc cggatgatcg aagaaaagat gttggtgctc ctgcgacagg gcaggatcag
     3121 caaatggttc agcggtattg gtcaggaagc gatagccgta ggcgcaaccc tggcgatgga
     3181 tccggaggaa tggatcctgc cgctgcatcg taacctcggc gtcttcacca gcaggggaat
     3241 gccgctttcg aaattgttcc tgcaatggca gggcgatgcc gatggctaca gcaaagggcg
     3301 cgagcgaagc tttcacttcg gaagccgcga acatgccatc tgcggcatga tctcccatct
     3361 gggccctcag ctgggcgtcg ccgatgggat cgcattggcg tataagctgc aaaaaaagaa
     3421 acaggcgacg ctggtgttca gcggggaagg cggcacgagc gagggagatt ttcatgaagc
     3481 cctcaatgtg gcatctgtct gggacctgcc ggtgatcttt ctgatcgaaa ataacggcta
     3541 tgccctgtcc acgcccgtct ccgaacaata ccgttgcgat agcctcgtct a
//
LOCUS       DATGKE010000721         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1400493, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000721 DATGKE010000000
VERSION     DATGKE010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400493"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1322)
                     /locus_tag="VKU83_12260"
     CDS             complement(108..1322)
                     /locus_tag="VKU83_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLI94384.1"
                     /translation="MSDLHPFALPAGHFALLLSLFVSLAGPLRAQVGKEAPQKDITDE
                     LHFLFRKKRTDTIQEVRKITFSGLPAVGYTLTTRAAITLTGNAVFRTDSDIANTKIST
                     ITSSAAYTENKQFSVPLETNIWTKRNKYNFVGDIHFMKYPQASFGLGSNSWIGNVDSM
                     QFNYIRFYEIVLRQITPDFFAGAGYIIDWRYHITDDNTRAPIPSDYILYGPQSTSLST
                     GFTLNALFDTRDNSINPYRGFYANGTYRNNLEFLGSNRNWQSLILDFRKYFRFPANSR
                     NILALWNYDWLVLDGKPPYLDLPSTNWDTYSSTGRGYIQGRFRGDEMVDFESEYRFPL
                     LPNGFLGAVIFVNGETFSGLDSHRLQSVQPGWGAGLRVKLNKTSRTNVDIDYGFGEQG
                     SNGLFVNIGELF"
     gene            complement(1477..1803)
                     /locus_tag="VKU83_12265"
     CDS             complement(1477..1803)
                     /locus_tag="VKU83_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94385.1"
                     /translation="MKTSFLLFALALGISRAEASDGAKVVKNHAPAPAVRNIVSTQLP
                     SKLLTTIKKNYKSYWITNLYKENLNGKVSYFITLENSDQTVKLNTTRSAGWSIIRVVP
                     KDINTH"
     gene            1969..>2977
                     /locus_tag="VKU83_12270"
     CDS             1969..>2977
                     /locus_tag="VKU83_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HLI94386.1"
                     /translation="MKVFGLLTIAVMGIIPVLAQEGPQVRTEGGIIEGTVEASGVHAF
                     KGIPYAAPPVGELRFREPQPVQPWDGVRKTTAFGPKAMQLPIFSDMKFRSNGMSEDCL
                     YLNVWQPASGTRLPVLVYFYGGGFVGGDGSEYRYDGESMAKRGIVAVTVNYRLGIFGF
                     LAHPDLTRESPHHSSGNYGLLDQHAALEWVQKNIAAFGGDPARVTIAGESAGSISVCA
                     QMASPLSKGLFAGAIGESGGLTGNIKPVSLEKAEQNGAAFQGGLGSKTIADLRAMPAE
                     ELLKAASKARFSSDIDGYFLPESPDEIYSAGDQMKVPLLAGWNSAEVPFGGVLGSAPP
                     TV"
BASE COUNT          677 a          692 c          933 g          675 t
ORIGIN      
        1 aggatcgccc gccggatcgg cgttcagaat ggggtcggca attggcagga gctggcggaa
       61 gccgccggct attgatggcc agtgatgggc acctggcggg cgccgggtca gaaaagctcg
      121 ccgatgttaa cgaaaaggcc attcgaacct tgctcgccga acccatagtc gatatccaca
      181 ttcgtacggg aggttttgtt cagcttgacg cgaagccccg caccccagcc gggctggacc
      241 gactggagcc ggtgggaatc gaggccggaa aaggtttcgc cgttgacaaa gatgactgcg
      301 ccaagaaaac cattgggaag tagcggaaac cggtattccg attcaaagtc gaccatctca
      361 tcgccccgga acctgccctg gatataaccg cggccggtgc tcgaatatgt gtcccaattg
      421 gtcgaaggga gatcgagata agggggcttg ccgtcgagca ccagccaatc gtagttccag
      481 agggcaagaa tattccgcga gttcgccggg aatcggaaat atttgcggaa gtcaaggatc
      541 agggactgcc agtttctgtt gctgcctagg aattccaggt tgttgcggta cgtaccgttg
      601 gcataaaaac cgcggtacgg gttgatcgaa ttatcgcggg tatcgaagag tgcgttcaag
      661 gtaaagccgg ttgatagcga cgtggactgc ggtccgtata ggatgtaatc agaaggaatg
      721 ggcgcgcggg tgttatcgtc agtaatatgg taccgccagt cgatgatata gcctgcgccg
      781 gcaaaaaagt ccggggtgat ctgccgaagg acgatctcat aaaagcggat atagttgaac
      841 tgcatcgagt cgacgttccc gatccaggaa ttgctgccca ggccgaagct ggcctgcgga
      901 tatttcatga aatggatatc gccgacgaaa ttatacttgt tgcgtttagt ccagatattc
      961 gtttcgaggg gcacggagaa ctgcttgttc tctgtatagg ccgcgctgga agtgatcgta
     1021 gagatctttg tgtttgcaat atccgaatcc gtccgaaaga ccgcgttacc ggtgagggtg
     1081 atggctgcgc gcgtggtaag ggtatagccg acggcgggca gcccggaaaa agtgatcttg
     1141 cgtacttcct gtatcgtgtc ggttctcttt ttacggaaaa ggaaatgcag ctcatccgtg
     1201 atatcttttt ggggcgcttc cttcccgact tgcgcccgaa ggggtcccgc caatgaaacg
     1261 aacaatgaca agagcaaggc aaaatgccca gccggcaagg cgaatggatg aaggtctgac
     1321 aactgtggcc ctgtttttgg ctattctatg caaaagttcg gctaacagtt gttaatgact
     1381 ggcaaatgcc actccgccgg atacaatagg cgaacaaggc cttgccatgc cggttagtca
     1441 tgcatagcgg ccttgtcctt ttgtattgat ctagggttag tgggtgttga tgtcttttgg
     1501 aacgacgcga ataatggacc atccggcaga ccgtgtggta ttaagcttca cggtctggtc
     1561 ggaattttcc agggtgatga aatacgacac cttaccattc aggttctcct tatagagatt
     1621 ggtgatccag tagctcttgt aattcttctt gatggtggtc aacagctttg aaggaagctg
     1681 ggtggaaacg atgttgcgga cggccggagc aggagcatga ttcttcacaa ctttggcgcc
     1741 atccgaggcc tcggcccggc tgataccaag ggccagggca aacagaagga aacttgtttt
     1801 cataagattt gttgctttct caggatttac agatttacgg cgtattaacg gggttcagcg
     1861 tcgaaacgta tgcgcaggcg ctcgaatcgg gaaaaaatca tggtaaatgg ggagatgagc
     1921 ggagcaaagt ccaaaaaaag ggctaacatt ggaacaaaat tttttggtat gaaggtgttt
     1981 ggcctactaa cgattgcagt tatgggaata attcccgttt tggctcagga agggccgcag
     2041 gtgaggacgg aaggaggcat tattgaaggg accgtagagg cctcgggcgt gcacgctttc
     2101 aagggcatcc cgtacgctgc gccaccggtt ggcgagttac gtttccggga gccgcagccg
     2161 gtgcagccct gggacggcgt ccggaagaca accgccttcg gaccaaaagc gatgcaactg
     2221 ccgatattta gcgatatgaa gttccgttcg aatgggatga gcgaggactg tctttatttg
     2281 aatgtttggc agccggcttc cggaacgcgg ttgccggtgc tcgtttactt ttatggcggc
     2341 gggtttgtag gcggggacgg gagcgaatac aggtatgatg gggaaagtat ggcgaagcgg
     2401 ggaattgtgg ctgtcacggt aaactaccgg ctggggatat ttgggttcct ggcgcatcct
     2461 gacctgacca gggagtcgcc gcatcattcg tcaggaaact acgggttgtt ggaccagcat
     2521 gcggcgttgg agtgggtgca aaaaaatatt gcggcattcg gcggcgaccc tgcgcgggta
     2581 acgattgccg gagaatcggc gggctcgatc tctgtgtgcg ctcaaatggc ctcgccgctg
     2641 tcaaagggtt tgtttgccgg cgctatcggc gagagcggcg gtttgaccgg gaatatcaag
     2701 ccggtatccc tcgagaaggc ggaacagaat ggggccgcct tccagggcgg cctcggatca
     2761 aaaaccatcg ccgacctccg cgccatgccg gccgaggagt tattaaaggc tgcttcgaag
     2821 gcccgctttt cttcggatat cgacggctac tttctgccgg agtccccgga tgagatctat
     2881 tccgcaggcg atcagatgaa agtgccgctg ctggcaggct ggaattcggc ggaggttccg
     2941 tttggcggcg tgctgggatc ggccccgcca acggtag
//
LOCUS       DATGKE010000722         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1191060, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000722 DATGKE010000000
VERSION     DATGKE010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191060"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VKU83_12275"
     CDS             <1..2020
                     /locus_tag="VKU83_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLI94387.1"
                     /translation="DSGFLKMFTFPLLRGDLSTALSRPFSMVVTRAFARKMFGDEEAL
                     GKTIRLDSVDIFTVTGVLADLPNNTLFNFEFLLPWSYMDKLKWDDSYWGNNSVQTYVL
                     LKKGVSQDRFDSRVRDITIDHTKGTADPSTTHVFSYPFAKQWLYSRNENGRLVAGRMV
                     TVRLFAVIAAFILLIACINFMNLSTARSEKRAREVGIRKVAGASKRSLMLHFMGESTL
                     ISFFAGVIAWAIVQLSLASFNSLVGKQLFLNYGDYRFWLYGLGFILLTGILAGSYPAL
                     FLSSFRPATVLKGAFRSLHAAVNPRKVLVVLQFTFAIILIICTMIISRQINYAEARNT
                     GYDRDRLVYSFLTGDVRKNYDLIKQGLLNSGAAIAVTKSMSPITQRYSDSWGYSWPGS
                     TKEDSKLDFVRMSADADFTKAMGITLLEGRDIDIRRFPTDSNAVLLNETAVRKMRLKT
                     PLGTLIRNDDEKWKVVGVVKDFIVESPFDPVNPMMINGPSSWFNVIHYKLNPAIPLQE
                     DLRRAEKVFRTYNPEYPFEYHFADEEYARKFDDSKRTGTLAALFAGLTVFISCLGLFG
                     LAMFMAAARMREIGVRKVLGASVANIAGLLSRDFLKLVSIAFLIAIPVAWYAMKQWLS
                     HYTYNTGIEWWIFLLAGVLSILIALATVSLQAIRSAVANPVKSLRTDG"
     gene            2095..>2403
                     /locus_tag="VKU83_12280"
     CDS             2095..>2403
                     /locus_tag="VKU83_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLI94388.1"
                     /translation="MKSKSVFGGFIFVVIFAIGLLVAYTAYNPAQPVISTAIGIVAFV
                     VALLVSAAIRIADPWDRAIVLRLGHYHSLRGPGLFLIIPIIDTIPYVVDTRVITTAFK
                     A"
BASE COUNT          535 a          610 c          632 g          626 t
ORIGIN      
        1 ggattccggc ttcctgaaga tgttcacttt tccgctgctg cggggcgatc tttctaccgc
       61 attaagcagg ccgttttcca tggtggtgac ccgggcattt gccaggaaaa tgtttggcga
      121 cgaggaagct ttggggaaaa cgatccggct ggattctgtg gatattttta ccgtaaccgg
      181 agtactggcg gacctgccga acaacaccct cttcaatttt gaattcttac tcccctggag
      241 ctacatggat aagttgaagt gggacgatag ctactggggc aataattccg tgcagactta
      301 tgtcttgttg aaaaaggggg tttcgcagga ccgctttgat tccagggtac gggacatcac
      361 catcgatcac acgaaaggca cggcagaccc atcaaccacg catgtcttca gctatccctt
      421 cgccaaacag tggttatact cgaggaacga gaacggccgc ctcgtagccg gcagaatggt
      481 tacggtcagg ctttttgccg tgattgctgc ctttattcta ctcatcgcct gcatcaactt
      541 tatgaatctt agcaccgcca gaagcgagaa acgggccagg gaagtcggga tccggaaagt
      601 ggccggagca agcaaaagga gcctgatgct tcattttatg ggtgagagca cgttgatttc
      661 gtttttcgcg ggggtcatcg cgtgggcaat cgtccagcta agtcttgcga gctttaactc
      721 gctggtgggt aaacaactgt tcttgaatta tggcgactac cggttttggc tgtatggact
      781 aggttttatc ctgttgaccg gcattctggc cggcagttat ccggcgcttt ttctctcgtc
      841 tttcaggccg gcaacggttc tgaaaggagc attcaggtcc ttgcatgccg ctgtcaaccc
      901 gcgaaaagtg ttggtcgtgc tgcaattcac tttcgcgatc atcctcatca tctgtacgat
      961 gatcatttcc cggcagatca attatgcgga agcgcgaaat accgggtatg accgggaccg
     1021 actcgtttat tcatttttaa ccggcgacgt gaggaagaat tatgacctca taaaacaggg
     1081 actgctcaat agtggagcgg ccattgccgt caccaaatcg atgagtccca tcacccaacg
     1141 ttatagtgat agctggggtt attcctggcc cggaagcacc aaagaggaca gcaagcttga
     1201 ttttgtgaga atgagcgctg acgctgactt tacaaaggcc atgggtatca ctctgcttga
     1261 gggcagagat atcgacatcc ggcgatttcc aaccgatagc aatgcggtat tgttgaacga
     1321 aaccgccgtc aggaaaatgc gactgaagac tcccctgggc acgcttattc gcaatgacga
     1381 tgaaaaatgg aaggtagtcg gcgtggtgaa ggatttcatt gtagaatcgc cttttgatcc
     1441 tgtcaacccg atgatgatca atggaccgtc aagctggttc aatgtcattc attacaagct
     1501 caatccggca attccgctgc aggaagacct gcggcgggcg gaaaaggtct ttaggaccta
     1561 taaccctgaa tacccttttg aatatcattt tgcggatgag gaatatgcca ggaaatttga
     1621 cgacagcaaa cgaaccggca ccctggcggc attgtttgca ggccttaccg ttttcatttc
     1681 ctgtctcggc ctgtttggcc tcgcaatgtt catggcggct gcgcggatgc gggaaatagg
     1741 agttcgaaaa gtactagggg ccagtgtggc caatatcgcc gggctcctgt caagagactt
     1801 tctcaagctg gtgtcgatcg cctttctcat cgcgattccg gtcgcctggt atgccatgaa
     1861 gcagtggctt tcccattaca cttacaatac aggaattgag tggtggatat ttttattggc
     1921 cggggttcta tccattttaa tcgcgctggc taccgtcagc cttcaggcca tccgctcggc
     1981 agtcgccaac cctgttaaaa gcctgagaac cgacgggtga tctgcatccg gaatcgttct
     2041 cgcttttttt ccccaccttt actactccta cttcatcaat tcaggagatt tatcatgaaa
     2101 agcaaaagtg tattcggcgg gttcatcttc gtagtgatat tcgccatcgg gttgttagtg
     2161 gcctacaccg cttacaaccc cgcccagccg gtgatcagca ccgccatcgg gatcgttgcg
     2221 tttgtcgtgg ccttgctggt ttccgccgcc atccggattg cggatccgtg ggatcgggcg
     2281 atcgttctgc gccttggcca ttaccattcg ctgcgcggac caggtctgtt cctgatcatt
     2341 cccatcatcg ataccatccc ttacgtggtc gacacccggg tgatcacgac tgccttcaag
     2401 gcg
//
LOCUS       DATGKE010000723         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1091004, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000723 DATGKE010000000
VERSION     DATGKE010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091004"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VKU83_12285"
     CDS             complement(<1..389)
                     /locus_tag="VKU83_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLI94389.1"
                     /translation="MSAEDPSDLPKKFSFHEEQQYHLMIEEVEDYAILMLDPQGVIRN
                     WNRGAEKIKGYTEQEIVGNHFSIFYPKEDREAGLPEQLIQRAAREGKAIHEGWRVRKN
                     GTKFWGSIVITALHDEIGNIVGFSKVTR"
     gene            417..821
                     /locus_tag="VKU83_12290"
     CDS             417..821
                     /locus_tag="VKU83_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLI94390.1"
                     /translation="MMVNEPYILIADDDPDDQELFEERLLRINPDAKIETVNNGMEAL
                     AFLRDRPAEALPKVIVVDYKMPGFTGYDFLVAIKNDPRYQAIPKVVWSTSNNQEYVQK
                     SMEGGAMRFITKPNDVRGFDQLVGEIDRLYQA"
     gene            complement(835..1095)
                     /locus_tag="VKU83_12295"
     CDS             complement(835..1095)
                     /locus_tag="VKU83_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94391.1"
                     /translation="MTTSSSTARQIREEIISDTKAFLAGLEAGATNEQLRSLLLRMRE
                     KELQLIKEGGTMIDPEMWRILHNRLAQKDGEEIIDNTAPPTT"
     gene            complement(1575..>2034)
                     /locus_tag="VKU83_12300"
     CDS             complement(1575..>2034)
                     /locus_tag="VKU83_12300"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HLI94392.1"
                     /translation="ATIEAAKRDRAAGKSASTQAGEFVKEEMDKIRKGKTGARNTKQA
                     IAIGLSEARRAGVALKPPAKGKAPEKTRRSAERELEKGQRHEPIDPARSRAREKVQQK
                     EPRTAATPQALSRHARAAAQERTPAERSVSARKAARTKKNSRGSGGSSST"
BASE COUNT          412 a          566 c          562 g          494 t
ORIGIN      
        1 cgggtaacct tggaaaaacc cacgatgttc ccaatctcgt cgtgcagggc ggtaatgacg
       61 atgctgcccc agaatttggt gccgttcttg cgaacgcgcc agccttcgtg gatggccttg
      121 ccttcccgtg cggcccgttg aatgagctgt tccggcaacc cggcctcccg gtcttccttg
      181 ggataaaaaa tgctgaaatg gttgcccacg atctcctgct ccgtgtagcc cttgatcttt
      241 tcggccccgc ggttccaatt acgtatcacg ccctgcggat ccagcatcag gatcgcataa
      301 tcctcgacct cttcgatcat caggtggtac tgctgctctt cgtgaaaact gaatttcttg
      361 ggcaaatctg aggggtcttc ggcagacatc ttcaaaaatt atggtaaatt tatcaaatga
      421 tggtgaacga accttatatc cttattgccg atgacgatcc cgacgaccag gagctgttcg
      481 aggagcgact cttacgcatc aatccggatg ccaaaataga aacagtgaat aacgggatgg
      541 aggcgctcgc ctttttgcgc gatcgccctg cggaggcctt gccgaaggtg atcgtggttg
      601 actacaaaat gcctggcttc acggggtatg attttctcgt cgccatcaaa aatgacccca
      661 ggtatcaggc cattccgaaa gtcgtttgga gcacgtcgaa caaccaggaa tatgtgcaga
      721 aatcgatgga gggcggggcc atgcggttta tcacaaagcc gaacgatgtt cgcggcttcg
      781 atcagctggt cggcgaaatc gaccggctgt accaggcata aagcgtagaa atgatcacgt
      841 ggttggcggc gccgtattgt cgatgatctc ctcgccgtcc ttctgagcta accggttatg
      901 gaggatccgc cacatttcag ggtcgatcat ggttcccccc tccttgatga gctgcagttc
      961 cttttcccgc atgcggagca gcaatgaccg caattgttcg ttggtagctc cggcctctaa
     1021 gccagctaag aaagctttgg tatccgaaat aatttcctcc cggatttgcc gggccgtcga
     1081 gctgctagtg gtcatagtgc ttgaatccga attgcaggta gtgccgcaaa gaccaaaccg
     1141 ctacttcccg ggtgcaccaa atgaaggaaa attaatttct gcaatcggtt gcaagagcgg
     1201 aattaggccg caagctgcac ccttcgccca gatttccagc ggattatgtt aagtagatct
     1261 acttaaacgg ccggaaagaa tgaatggtcc gcgcaataaa acactaaaac agacgctaga
     1321 ataggaagaa ttgccccatc ccgcgggcga ccggatcgaa tgcctgatga cgtgtaaaac
     1381 aagctggccc gcctatcgct gtcgggtccg cggcatcggg agacgaagcc agacgtgcag
     1441 gtaaaacaag ctgatccgtt ccctacgcga tccctgaacg tcgatattgc acgcgtggaa
     1501 tgtggttgtt cgctcagcca tcctatcgaa aaaccgaagg ctttgcccct gacgaaaaat
     1561 atttcgcccg cagcttaggt tgaagagctc ccgccagacc cgcgggagtt ttttttggtg
     1621 cgggctgcct tgcgggccga cacactgcgt tctgcgggtg tccgctcctg ggcggcggcg
     1681 cgggcatgcc tcgaaagggc ctgcggcgtt gctgcggtcc gcggttcctt ctgttgcacc
     1741 ttttccctgg cgcggctgcg tgcgggatcg atgggctcgt ggcgctgtcc tttttcaagt
     1801 tcccgttccg cgcttctgcg cgtcttttca ggcgccttgc cttttgcggg aggtttcagg
     1861 gcgacgccgg cccgtcgcgc ttcggaaagt ccgatggcaa tggcctgctt cgtattgcgc
     1921 gccccggttt tgcccttccg gatcttgtcc atttcctctt tgacgaattc gccggcctgg
     1981 gtgctcgcgg atttccccgc ggcccggtct cttttagctg cttcgatggt tgct
//
LOCUS       DATGKE010000724         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1094689, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000724 DATGKE010000000
VERSION     DATGKE010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094689"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VKU83_12305"
     CDS             <1..2113
                     /locus_tag="VKU83_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HLI94393.1"
                     /translation="KRPIQSTYLIDSANFIACHHFNYLNKYDILKDAAEGAVFLLNAP
                     YAKEELWTKLPRPIQEEIVRKKLKFYAINAHAVAKECDMGGRINTILQTCFFAISNIL
                     PRDEAIQKIKAAIDETYGKKGEAILEKNYAAVDKTLANLYEVNYDTSGLGEIPLSNTV
                     PDSAPAFVRQVLGKIIAGGGDELPVSAFPADGSYPSGTTKWEKRNIADAVPVWDTSLC
                     TQCNKCVLICPHAAIRVKVYDKALLANAPAGFKHMDPIGKEWDKNTESYTLQVSTDDC
                     TGCTLCMEYCPITSKTDAGHKAVNMYDKTDIQDAERLNWEYFLSLPEVGRERINRNTV
                     KGSQFFQPLFEFSGACSGCGETPYLKLLTQLYGERMIVANATGCSSIFGGNLPTTPWS
                     KTKEGRGPAWANSLFEDNAEFGLGIRLANEKKREAAIAILQQLREEVGGELAEGIIHA
                     SETNEAEINGKNQLIKQLKMRLATVDTSAAKNLSYLADFLAEKSMWIVGGDGWAYDIG
                     YGGLDHVLSTGENVNIFVMDTEVYSNTGGQKSKSTPIGASAKFSVNGKTTGKKDLALQ
                     AIAHGTAYVAQIAMGANDSHTVRTIIEAENFPGPSLIIAYAHCIAHGIEMSNGAEDQD
                     LAVKTGYWPLFHYNPLKPRGQRFVIDSKEPSLPLADFLYRENRFNVIRAKDPALAAQF
                     LTEAADGVKERWARLKTLTAL"
     gene            2232..2696
                     /locus_tag="VKU83_12310"
     CDS             2232..2696
                     /locus_tag="VKU83_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HLI94394.1"
                     /translation="MEAITSKETASVLEDLIKINNDRIAGYEKALKDLKPEDSDLRML
                     FLKAIDQSRSIRTRLGTEIQTLGSSIPTDTSASGSIHRAWLDVKATFTGHGRKAILSS
                     CEFGEDAIQKAYETALSSEHLPEYLSAVVLAEKQELMIVHNEIKALRDQSSD"
     gene            3033..3365
                     /locus_tag="VKU83_12315"
     CDS             3033..3365
                     /locus_tag="VKU83_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLI94395.1"
                     /translation="MKLFVGGLPFSVDDRGLQEMFESYGTVGSAKVITDRETGKSRGF
                     GFVEFSTDADGEAAIKALNGGTFEGRQLTVKVAEDKPRGGGNGGFRGGNDRRGGGSGG
                     FGRGRDRY"
BASE COUNT          916 a          914 c          918 g          715 t
ORIGIN      
        1 aaagcgtccc atccagtcga catacctgat cgattcagca aacttcattg cctgccacca
       61 tttcaattac ctgaacaaat acgatattct gaaagacgcg gcggaaggcg ctgtcttcct
      121 tctaaacgca ccctatgcca aagaggaatt gtggacgaaa cttccgcggc cgatccagga
      181 ggaaattgtc cggaagaaac tgaagtttta tgccataaat gcacacgccg tcgcaaaaga
      241 atgtgacatg ggcggtcgta tcaacacgat cctgcaaact tgtttttttg ccatcagcaa
      301 tattctaccg cgcgatgaag cgatccagaa gataaaggca gcgattgatg agacctatgg
      361 gaagaagggg gaagcgatcc tggaaaagaa ttacgccgcg gtagacaaga cgctggctaa
      421 tttgtacgaa gtgaattatg acacatccgg tcttggagaa atacccctga gcaacaccgt
      481 tccggattct gccccggcat ttgtaagaca ggtcctggga aagattattg cgggcggcgg
      541 ggacgaactc ccggtcagcg cctttccggc cgatggcagt tacccgtccg gtacgaccaa
      601 atgggaaaaa cgaaatatcg ccgacgctgt ccccgtttgg gacacgagtc tttgtaccca
      661 gtgcaacaag tgcgtgctga tttgtcccca tgccgccatc cgcgtcaaag tgtacgataa
      721 agccctgttg gcgaatgctc ccgccggctt caaacatatg gaccccatcg gcaaagaatg
      781 ggataagaat acggaatcct ataccctgca ggtctccacc gatgattgca ccggttgcac
      841 cctttgcatg gagtactgcc cgatcaccag taagaccgat gccggccata aagccgtcaa
      901 tatgtatgat aagacagaca ttcaggacgc cgaacggctg aactgggaat attttctaag
      961 ccttcccgaa gtcggcagag agcgaattaa ccgaaatacc gtaaagggat cgcaattttt
     1021 tcaaccattg ttcgagttct cgggcgcctg ctcgggctgc ggtgaaacgc catacttgaa
     1081 attgttgacg caactctatg gcgaaaggat gatcgtggcg aatgccacag gctgttcttc
     1141 tatttttggc ggcaatcttc cgaccactcc ctggtcaaaa acgaaggaag gcaggggacc
     1201 cgcctgggcg aattctttgt tcgaagacaa cgcggaattc gggctgggca tccgccttgc
     1261 caatgaaaag aagcgggagg ccgcgattgc gatcttgcag cagctccggg aagaagtggg
     1321 tggagagctt gcggagggca ttattcatgc ctcggaaacg aatgaagctg aaataaacgg
     1381 gaagaatcag ctgattaaac aactgaagat gcgcctcgcc accgtcgaca ccagcgctgc
     1441 aaagaacctt tcctacctcg cagactttct ggcggagaaa tccatgtgga tcgtcggcgg
     1501 cgacggctgg gcttacgata tcggctatgg cggcctggac cacgtgcttt caaccggaga
     1561 aaacgtgaac attttcgtca tggatacgga ggtctattcc aacaccggcg gtcaaaaatc
     1621 aaaatccact ccgattggcg ccagcgccaa attctccgtg aatgggaaaa caaccggcaa
     1681 aaaagacctt gccttgcaag ccattgccca cgggaccgca tacgtagcgc agattgcgat
     1741 gggcgcaaac gactcccata ccgtccgaac gatcatcgaa gcggagaatt tccccggtcc
     1801 ctcacttatc attgcctatg cccattgtat cgcccatggt atcgaaatga gcaacggcgc
     1861 ggaagatcag gacctggccg tcaaaaccgg ttattggcct ttgtttcact ataaccccct
     1921 caaaccaagg ggtcaacggt ttgtgatcga ctcaaaagag ccgtcgcttc ccctcgccga
     1981 tttcttgtac cgtgaaaacc gattcaatgt gatcagggcg aaggatcccg cgctggctgc
     2041 ccaatttctg accgaggcag cggatggggt aaaagaacgg tgggcaagac taaaaacatt
     2101 aactgctttg taaaagcagc ccgggactcc gtggggagca ttttccccgt tgggcgctga
     2161 tcctggctta atcgccgggc cggcggaccc tgatggcaca gttttttttt cggctatacg
     2221 aaacatcaca tatggaagca attacatcca aagaaaccgc aagcgtgctc gaagacctga
     2281 taaaaatcaa caacgaccgg attgcaggtt acgaaaaagc actcaaggac ctgaaaccgg
     2341 aagactcgga cctgcgcatg ctctttctca aagcaatcga tcaaagcaga tccattcgca
     2401 caaggctggg cacggagata caaacgctgg gttcgtccat tcccacagac acatccgcta
     2461 gcgggtcgat ccaccgcgct tggctggacg ttaaagccac tttcaccggg cacggccgga
     2521 aagccattct gtcaagctgc gaatttggcg aagacgccat ccagaaggcc tacgaaacag
     2581 ccctgtcgag tgagcacctg cccgaatatc tgtccgctgt cgttctggct gaaaaacagg
     2641 aattgatgat cgtgcataac gagatcaagg ccttacgcga tcagagttct gattaaattc
     2701 gcttcggcgg cgttcttaca ggaggcttac cgccgcctga aaataaggaa cagaataacg
     2761 atcaatacga gggttccaat tccaatgtac atagtcttca gtttggataa tagaatggtt
     2821 ttgccaatta cactaataaa aaagcatgcc ggactccaaa gctgaaaaac tatgtttttt
     2881 agcatggctt tgtcccttac ccgccggtgc ccggttcgcc ggctggccgc gacccggccg
     2941 caacccggcc gcaactgaaa taaggtgtat aaaggcggga aaaaacgtgc ctcatgctaa
     3001 cgcgcagaag cgtaccttca ccaaaaacaa gtatgaaact atttgtcggg gggctcccct
     3061 ttagtgtcga tgaccgggga ctgcaggaga tgttcgagtc ttacggtacc gtaggttccg
     3121 ctaaagtcat cacggatcgg gaaaccggga aaagccgggg tttcgggttc gttgaatttt
     3181 caaccgatgc cgacggcgag gcagcaatca aggccctgaa cggtggcact ttcgaaggac
     3241 gacaactgac cgtcaaggtg gctgaagaca agccaagggg cggagggaac ggcggctttc
     3301 gcggaggaaa cgaccgtcgc ggcggcggca gcggcgggtt tggccggggc agggatcgct
     3361 actgatttcg accgcccgcc gcgcccggag catttaatgg catcgggatt acaacatcac
     3421 cttcctggcg actgaccact ggctaccatc ggtaacctcg aca
//
LOCUS       DATGKE010000725         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_158988, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000725 DATGKE010000000
VERSION     DATGKE010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158988"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="VKU83_12320"
     CDS             <1..2162
                     /locus_tag="VKU83_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HLI94396.1"
                     /translation="LRHVNQMGGFDCPSCAWPDPDDERSGVAEYCENGAKAIAEEITT
                     KKLSAGFFAEHSVSQLASLTDYEIGGKGRIAEPLLLSKGATHYRPVSWDDAFHLIATT
                     LNALSSPNEAIFYTSGRTSNEAAFLYQLFAREYGTNNLPDCSNMCHESSGVALTESLG
                     LGKGSVRLDDLYLAEVIIIMGQNPGTNAPRMLTALQKAKHNGARIISVNPLPETGLMA
                     FANPQQAGGILGLSTPLTDLYLPVRLNGDMALLQAIQLLLWLEERQAPGTVFDLAFIR
                     ERTTGYEPWLQHLQQASLPELSAACGVSIELLDQAANLIKGKTKIIVCWAMGLTQHTN
                     AVNTIREIVNLLLLKGSIGKPGAGTCPVRGHSNVQGDRTVGIWERPTKQLLDSIENVF
                     GFQPPREPGFDVVNAIRAMHDGRASVFFAMGGNFLSATPDTTFTAEALRRCRLTVHVS
                     TKLNRSHLVHGESAIILPCLARTDKDRTGGREQFVSCENSMGVVQLSKGVLEPISNQL
                     LSEPAIVCRLAKATLAARTRLDWAKYELNYDRIREDIERTIPGFSDYNRRVREPGGFY
                     LPNGPRDGIFNTPDQKAHFNVADPRPLRLASGELMLMTVRSHDQFNTTIYGLNDRYRG
                     IYNERRVVLLNAEDMREQGIAPDDKVDICNKHGGVERTAYGFIAVPYPIPRGCAAAYY
                     PETNVLVPIDAVADKSNTPVSKLIIIRLRPATAATEA"
     gene            complement(2188..>2957)
                     /locus_tag="VKU83_12325"
     CDS             complement(2188..>2957)
                     /locus_tag="VKU83_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94397.1"
                     /translation="RVPYYAATAWYHKALAPDLQAKDLTDMLPEVENFTVDELIPALA
                     KGGFLDSSKKKELAAKLSRYTGLTEKDVLDQNLIIPADFFWKALLRDKGFTVGRLDSR
                     YRGIDSKAAGDAPDYNAELTSWLHSFTPAINIYLREDLHYKTDLKYNMFGPVFPWDNR
                     NDHVGENLRKAIAENPFLHLLIQSGYYDGACDYFNAKYSLWQLDPSGKLKDRLSWQGY
                     RSGHMLYLRKQDLSDANENLRKFIEGALPKPGEAAKY"
BASE COUNT          629 a          952 c          775 g          601 t
ORIGIN      
        1 cccttcggca cgtcaaccaa atgggaggtt tcgactgtcc aagctgcgca tggcccgacc
       61 cggacgacga acgctccggt gttgccgaat attgcgagaa cggcgctaag gccatcgcag
      121 aagagatcac taccaaaaaa ctcagcgccg gtttttttgc cgaacattcc gtatcccaac
      181 tggcaagtct gaccgattac gagatcggcg gcaaaggtcg catcgccgaa ccccttcttc
      241 tttcgaaagg cgcgacgcat tataggccgg tctcctggga tgacgctttt catctcatcg
      301 ccacgaccct caatgccctc tcctcgccaa acgaagcgat cttctacact tccggccgaa
      361 ccagtaacga agccgccttt ctctaccagc tcttcgcccg tgaatacggg accaacaacc
      421 tgccagactg ttcgaatatg tgccacgagt cgagcggcgt agccctcacc gaatccctcg
      481 gtctggggaa aggttccgtc cgcctcgacg atctatatct cgcggaggtt attatcatca
      541 tgggccaaaa cccgggcacc aacgcgccgc ggatgctcac tgccctccaa aaagcgaaac
      601 acaacggcgc caggatcatt tcagtcaacc cgctgcccga aaccggcttg atggcttttg
      661 caaacccgca acaggcaggg ggcatccttg gactttccac cccgctcacg gacctttacc
      721 tgcccgtcag gctcaatggc gacatggccc tcctgcaggc catccaactg ctgctatggc
      781 tcgaggagcg ccaggccccg ggaacggtct tcgacctcgc attcatccgg gaacgcacca
      841 caggttatga accctggcta cagcatctcc agcaagcttc cctgcctgag ctctccgccg
      901 cctgcggcgt ctccattgaa ctgctcgatc aagccgcgaa cctcataaag ggaaaaacaa
      961 agatcattgt ctgctgggct atgggcctca cccaacatac caatgccgtc aacaccatcc
     1021 gggagatcgt taacctgctc ctgttgaagg gcagcatcgg caagccagga gccggcacct
     1081 gccccgtgcg tggccacagc aatgtccagg gcgaccgcac cgttggcatc tgggaacgcc
     1141 cgaccaagca actgctggac agcattgaga acgtctttgg tttccagcct ccccgtgaac
     1201 cggggttcga tgtcgtcaac gccatccggg cgatgcacga cggccgcgct tcggtattct
     1261 tcgcaatggg cggcaacttc ctgtccgcca cacccgatac gacatttacc gcggaagcgc
     1321 tccgccgctg tcgcctcacc gtccatgttt ccacgaagct gaaccgcagc catctcgtac
     1381 acggcgaatc ggcgatcatt ctcccgtgcc tggcacggac cgataaggat cggaccggcg
     1441 ggcgggaaca gttcgtttcc tgtgaaaatt ccatgggcgt cgtccagctt tcgaaagggg
     1501 tcctggaacc catctcgaac cagcttctca gcgaaccggc aatcgtctgc cgcctcgcca
     1561 aggccacgct cgccgcccgg accagattag attgggcgaa atacgaattg aactacgacc
     1621 ggatccgtga ggatatcgaa cgcaccattc ccgggttttc cgattacaat cgccgggtgc
     1681 gcgagcccgg cggcttctac ctcccgaatg ggccacgaga cggcattttc aacacgccgg
     1741 accaaaaggc tcatttcaat gttgccgacc cccggcccct ccgcctcgcg tccggcgagt
     1801 taatgctcat gacggtccgc agccatgacc agtttaacac cacgatctat ggattgaatg
     1861 accgctaccg cggcatctat aatgagcgcc gggtggtcct gctgaatgcg gaagatatgc
     1921 gggaacaggg aattgcgcct gacgacaagg tggatatctg caataagcat ggcggagtcg
     1981 agcgaacggc ttatggtttt attgccgtcc cttacccgat cccccggggg tgcgcggcgg
     2041 cctattatcc cgagacgaac gttcttgttc ctatcgatgc cgtagccgat aagtcgaaca
     2101 cacccgtttc caagctcatt atcatcaggc ttcggccggc gactgcggcg accgaagctt
     2161 aacgaaggcg gcatcaagcc gccgcggtca gtactttgcc gcctcaccgg gctttggaag
     2221 cgccccctcg ataaacttcc gcaaattttc atttgcatcc gacagatcct gcttgcgcag
     2281 gtataacata tgcccgctcc ggtaaccttg ccagctcagc ctgtccttga gcttcccgct
     2341 gggatcgagc tgccacaaac tgtatttcgc gttgaaataa tcgcaggcgc cgtcgtaata
     2401 gccggactgg atcagcaagt gcaggaacgg attctccgcg atcgcctttc gcaaattctc
     2461 gcccacatgg tcattccggt tgtcccaggg aaagacaggg ccgaacatat tgtatttcaa
     2521 atcggtcttg taatggaggt cctcccgcag gtagatattg atcgcgggcg tgaacgaatg
     2581 cagccaggaa gtcagctcgg cgttgtaatc cggcgcgtct cccgccgcct tgctgtcgat
     2641 cccgcgatac cgtgaatcca gccggccgac cgtaaaaccc ttgtcacgga gaagcgcctt
     2701 ccagaaaaag tccgcaggga tgatcaggtt ctgatcgagc acgtcttttt cggtcagccc
     2761 ggtgtaacgg gagagcttag ccgccagctc cttcttcttg ctgctgtcga ggaaccctcc
     2821 cttcgcaagc gccggtatca gctcgtcaac ggtaaagttc tccacctccg gcagcatgtc
     2881 ggtcaggtcc ttcgcctgca gatccggcgc cagtgcttta tggtaccagg ccgtggcggc
     2941 ataatagggt acccgca
//
LOCUS       DATGKE010000726         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1016919, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000726 DATGKE010000000
VERSION     DATGKE010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016919"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /gene="dnaN"
                     /locus_tag="VKU83_12330"
     CDS             <1..638
                     /gene="dnaN"
                     /locus_tag="VKU83_12330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLI94398.1"
                     /translation="MDKKGLQFVATDAHRLVRYRRKDVSCPKSDSLIVPKKPLSLLKS
                     ALPANEDEITISYNSNHLFVTHGTTQMSCRLIDARFPDYKVVIPADNPYKLTVGRNDF
                     QGALRRVSIFSNKSTNQVVLNIHGSELQLTAQDVDFSFEGNERMKCQYDGEDLAIAFN
                     ARFLIEMLAAADSDEVRMELSTPTKAGIIKPTDQDENEEVLMLVMPLMLNS"
     gene            664..909
                     /locus_tag="VKU83_12335"
     CDS             664..909
                     /locus_tag="VKU83_12335"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLI94399.1"
                     /translation="MIQFVQIRAYDNYISAHIVLARLQDAGINCHLKDEFTVTIDPLL
                     SPALGGIKVMVAEPQVERALRILAEVNDNEKPDQKQV"
     gene            906..1634
                     /gene="kdsB"
                     /locus_tag="VKU83_12340"
     CDS             906..1634
                     /gene="kdsB"
                     /locus_tag="VKU83_12340"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR00466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HLI94400.1"
                     /translation="MKTIAMIPARYAATRFPAKLMQLLGNKTVIRHTYDNTVATGLFD
                     EVIVVTDSDLIFQEISGHGGAAARSRKEHQSGTDRIAEAVAGMDADIIVNVQGDEPFV
                     QKAALEHLLALFQGEAGGQVLVASLVQVLKEQRFIHDPNYVKVALDKNNNALFFSRSV
                     IPYPRDPAASPVYYEHIGVYAFRKQALLQFTAWPVSPLEAAEKIECLRYLENGVPIRM
                     AITQYMGIEIDTPEDLQRAAGLLQ"
     gene            1661..>2305
                     /locus_tag="VKU83_12345"
     CDS             1661..>2305
                     /locus_tag="VKU83_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLI94401.1"
                     /translation="MNQPLWGIDLGGTKIEGVILPSVHEPRPLVRTRIDTEAAKGYEH
                     VIGQLHRLVQEMERQSGLQPRAIGIGTPGILDPSLHVMKNCNTVILNGKPLRDDLAAK
                     LGIRVELANDANCFALAETHWGVVKAEKPDAAVVFGIIMGTGVGGGIVLDGKIWAGRH
                     GIAGEWGHNYLDDSGGPCYCGKAGCVETVISGPATERFYARLAGKTLKLREIAAR"
BASE COUNT          532 a          644 c          664 g          465 t
ORIGIN      
        1 aaatggataa gaagggattg cagttcgtcg ccacggatgc ccaccggctc gtccgctaca
       61 ggcgcaagga cgtgtcctgc ccgaaaagcg attcactgat cgttcccaag aaaccgctca
      121 gtctcttgaa gtccgcgttg ccggccaacg aagatgagat cacgatcagc tataacagca
      181 accatttgtt cgtcacgcat ggtacgaccc aaatgagctg tcgcctgatc gacgcgcgtt
      241 tccccgatta taaggtcgtc attcccgccg acaaccccta caagctgacg gttggccgaa
      301 acgactttca gggcgcattg cgccgcgtca gtatcttcag taataagagc actaaccagg
      361 tcgtgctcaa tattcatggc agcgaattgc agttgaccgc gcaggatgtg gacttttctt
      421 ttgaaggcaa cgaacgcatg aaatgccaat acgacggcga ggacctcgca atcgccttta
      481 acgcgcggtt cctgatcgaa atgctggccg ctgctgactc cgatgaggtc cggatggaat
      541 tgtcgacgcc gacgaaggcg gggatcatca agcccaccga tcaggacgag aacgaggaag
      601 ttttaatgct ggtcatgccg ctgatgctga acagctagcc gcggtaaccc gggcaggtcc
      661 cttatgatcc agtttgtgca gataagagct tacgacaact atatctccgc gcatatcgtg
      721 cttgcccggc tgcaggatgc cggtatcaat tgccatttga aggacgagtt caccgtcacc
      781 atcgatccgc tgctaagccc ggcccttggc ggcatcaagg taatggtggc tgaaccccag
      841 gtggagaggg ccctcaggat cctcgcagaa gtcaatgaca atgaaaagcc ggatcaaaaa
      901 caggtatgaa gaccattgca atgatccccg cccgctatgc ggcgacccgt ttccccgcca
      961 agttgatgca gttgctcggc aataaaaccg ttatccggca cacctacgac aatacggtgg
     1021 ctacaggcct cttcgatgaa gttattgtcg tcaccgacag cgacctgata tttcaggaga
     1081 tttcaggtca tggcggagcg gcggcaagga gcaggaagga acaccaaagc ggcaccgacc
     1141 ggattgctga agccgtcgcc ggtatggacg ccgatattat cgtaaatgtc cagggggatg
     1201 agccttttgt gcaaaaagca gcattggagc atttgctggc cttgttccag ggcgaggcgg
     1261 gtggccaggt actggtagcc tcgctcgttc aggtcctgaa agaacagcgg ttcatccatg
     1321 atcccaatta tgtcaaggtg gccctcgaca agaacaacaa tgcgctgttc tttagccgca
     1381 gcgtcattcc ctatccccgc gatcccgccg cttcgccggt ttattacgaa catatcggtg
     1441 tttatgcctt tcgcaagcag gcgttgctgc agttcacggc ctggcctgtg agcccgctgg
     1501 aggccgccga gaagatcgag tgtttgcgat acctggagaa cggcgtcccg atccggatgg
     1561 caataacgca atatatgggg atcgagattg acacgccgga agaccttcag cgggcagccg
     1621 gtttattgca ataaccaacc gataaaagtt acattcagcc atgaaccagc cactatgggg
     1681 aattgatctt gggggcacca aaatcgaggg ggtcatcctc ccttccgtcc acgagccgcg
     1741 tccgcttgtt cgcacccgca tcgatacgga agccgctaaa ggctacgagc acgtcatcgg
     1801 ccagcttcac cggctcgtgc aggaaatgga acggcagtcg ggtctacagc cgcgggcaat
     1861 cgggatcggc acccccggga tcctcgatcc ttcgcttcac gtgatgaaga actgcaacac
     1921 cgtcatcctc aatggcaaac ccttacggga tgacctggct gcaaagcttg ggatccgggt
     1981 cgaacttgcc aacgacgcca attgcttcgc cctggcggag acccactggg gcgtcgtgaa
     2041 ggccgaaaaa cccgatgcgg ccgtggtctt cggcatcatc atgggcacgg gtgtcggcgg
     2101 cgggatcgtc ctcgacggga agatctgggc cgggcgccac ggcatcgcgg gggaatgggg
     2161 acataattac ctggatgact ccggcggtcc ctgttattgc ggaaaagccg gttgtgtcga
     2221 aacggtcatc tccggaccgg cgaccgagag gttttatgcg aggcttgcgg ggaaaacgct
     2281 gaagctgcgg gaaattgccg cccgg
//
LOCUS       DATGKE010000727         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1403010, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000727 DATGKE010000000
VERSION     DATGKE010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403010"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VKU83_12350"
     CDS             <1..360
                     /locus_tag="VKU83_12350"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLI94402.1"
                     /translation="NYGHTKDTIKPQVDLYNIDGKDIIVLAEGRLVNLGCATGHPSFV
                     MSNSFSNQTLAQIELWTNSSKYENKVYVLPKHLDEKVARLHLAKIGVELDELSDAQAA
                     YLGIPKNGPFKPEHYRY"
     gene            complement(415..1401)
                     /locus_tag="VKU83_12355"
     CDS             complement(415..1401)
                     /locus_tag="VKU83_12355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HLI94403.1"
                     /translation="MQQKILIARPTFPDIMDRLRQHFDVTLNDGPRYTPGQLNAALRD
                     MDGALISGGDKIDATTLEGLTRLKAVCVTAAGFNNVDVAALTKAGVLGTNSPGPADET
                     VADFTLGLMIATARRLVEASLSVVQGQWKESFPIRFFGINICDKTLGILGMGGIGQAV
                     ARRVSGFRMPVIYHNRRRLAPETEAACRAEYRSKDDVLRQADFLLLAMPFNAANYHLI
                     GAAELKLMKPSAMIFNIARGGLIDEAALASALRRKELAAAGLDVFEHEPVIHPDLVGL
                     PNVVLTPHIAGATEEAQHGLAAMAADNLITALTVGPQAVRRAAVFNPEVLGA"
     gene            1513..1695
                     /locus_tag="VKU83_12360"
     CDS             1513..1695
                     /locus_tag="VKU83_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94404.1"
                     /translation="MKLLITVMMCIAVAVSGLFSLHYFRASVYDLSWLLDITAYGSIV
                     AGIYLITAQKRKRLLQ"
     gene            1818..>2350
                     /locus_tag="VKU83_12365"
     CDS             1818..>2350
                     /locus_tag="VKU83_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94405.1"
                     /translation="MKRSIVRWLRDVSIAKKLYFTVGIMALLIAIELGVLMFSINTLS
                     SVRAYVGGEGLWSKAQKDGLYQLFEYARTRDEADYRKFEAFMKVNLGDHKARIELSKG
                     AGADLNIVRQGFIEGRNNPDDVDGMIKLFLRFHGNRYISRAIMAWTSADSVIADFITL
                     GKKLQEEVQTQPFSQQRV"
BASE COUNT          536 a          660 c          646 g          508 t
ORIGIN      
        1 aactacggcc ataccaagga caccatcaag ccccaggttg acctgtacaa tatcgacgga
       61 aaggacatca tcgtgctggc tgaaggccgc ctggtcaacc ttggctgcgc cacgggccat
      121 ccttctttcg tgatgagtaa ctccttctcc aaccaaacgc tggcgcagat cgagctatgg
      181 accaactcca gcaagtacga gaacaaggtt tatgttctgc ccaagcatct cgatgagaag
      241 gtcgcccgcc tgcacctcgc aaagatcggc gtagaactgg acgaactttc ggacgcacag
      301 gctgcatacc tgggcattcc gaagaacggg ccgttcaaac cggaacacta tcggtattaa
      361 gcgcgatttt ggcccggcca ataaatgagc ccgtctctgc ggaggcgggc tttcttatgc
      421 ccccagcact tccgggttga agaccgccgc ccggcgcacg gcctgcggcc ccacggtcag
      481 tgccgtgatc aggttatccg ccgccatagc cgccaggcca tgctgcgcct cctcggtggc
      541 accggcgata tgcggggtca ggacgacgtt gggcagccca accaggtccg gatgaatgac
      601 gggttcatgc tcaaaaacat cgagaccagc cgctgccagc tcctttcgcc gcagcgccga
      661 cgccagcgcg gcttcgtcga tgagaccgcc gcgggcgata ttgaagatca tggcgcttgg
      721 cttcatgagc ttaagttccg ccgctccgat gagatgatag ttggcggcgt tgaagggcat
      781 ggccagcagt agaaagtcag cctggcgcaa aacatcgtcc ttactgcggt attccgcacg
      841 gcaggcagcc tctgtctcgg gcgccagcct gcgcctgtta tgatagatca ccggcatgcg
      901 gaaaccggaa accctccgtg cgaccgcttg tccaatcccg cccatgccga gaattccaag
      961 cgtcttatcg cagatattaa ttccaaaaaa acggatggga aaggactcct tccattgacc
     1021 ctggacaacg gacaagctgg cttccacgag tcggcgtgcg gtagcgatca tcagacccag
     1081 cgtaaaatca gctaccgttt cgtcggcagg gccgggagaa ttggttccca ggacgccggc
     1141 ttttgtcagc gctgcgacat cgacattatt gaaccccgcg gccgtaacac aaaccgcttt
     1201 cagccgggtc aacccttcca gcgtggtagc atcgatctta tcgcccccgg agatgagggc
     1261 tccgtccatg tcccgcaggg ctgcgttaag ttgtcctggc gtgtatcgcg ggccgtcatt
     1321 gagggtcaca tcgaagtgtt gtctcagccg gtccattatg tcggggaagg taggcctcgc
     1381 gatcaggatt ttctgttgca tgggcaaatg taaaaattca ttaacaattc gacccttctt
     1441 tacaaatcgt tagtaaacga acccgaattc gcacggtccg ttatgtaagt agatcaccat
     1501 aaaactacaa ctatgaaact gttaataact gttatgatgt gcatcgcggt agcggtcagc
     1561 ggcttgttta gccttcatta tttccgggcg tcggtctatg acctctcctg gttgctggat
     1621 attacggcat atggctccat cgtagccgga atctacctta tcaccgctca aaagagaaaa
     1681 cgtcttttgc aatagtcacc gcatcggttt aggataacgg agaggggtcc gatggccaaa
     1741 aggctgccgg gccctttgtt tttacgggcc aattctgtat tttggccagc ccgtcctgct
     1801 cgcgcaaaaa tgacagcatg aaaagatcga tcgtacgatg gttgagagac gtctccatag
     1861 ctaaaaagct ctattttacg gtcgggatca tggcgctgct gattgccatt gaactgggtg
     1921 tactcatgtt ctctatcaac acgctctcca gcgtccgcgc ctatgtcggc ggggaagggc
     1981 tctggtcgaa agcgcaaaag gacggtctgt atcagctctt cgagtatgcg cgcacccgtg
     2041 acgaagccga ctaccggaag ttcgaagctt ttatgaaggt caacctgggc gaccacaaag
     2101 cccggatcga gttaagcaaa ggggccggtg cggaccttaa catcgtccgg caagggttta
     2161 tcgagggccg gaacaacccg gacgatgtgg acgggatgat caaactgttc ctccggtttc
     2221 atggcaacag gtatatttca cgggccatta tggcctggac gagtgcggac agcgtcatcg
     2281 ccgattttat caccctgggg aaaaaattgc aggaggaggt ccaaacgcag cccttctcgc
     2341 agcaacgggt
//
LOCUS       DATGKE010000728         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_55178, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000728 DATGKE010000000
VERSION     DATGKE010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55178"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VKU83_12370"
     CDS             <1..1239
                     /locus_tag="VKU83_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLI94406.1"
                     /translation="GEQHQVNALFEHATEGIVLTDDKGRMVLLNPAALQLFEYNREEL
                     LGQPIEMLIPKRFSPRHTDYRHGFYQAPGNRTMGHGRDLFARKKDGSEFPVEVSLSYY
                     RQKGSMYVIAFVVDITARKQSEKELLDRKSQLEKIMEDVRRLNAELEGKVEERTLVLR
                     EALQELEKSQQELHEALDKERQLNEIKSRFVSMASHEFRTPLSTVLSSAALLSKYPTT
                     DQQVVRERHIRKIKDSVKQLNDLLGDFLSLGKLEEGKVIADPSPFDVKDLLDETVEEM
                     RSLSKDGQNIHCNFTGDRSFLTDKRLLKNIVINLLGNAMKFSPDGASIWLEVKRRPGV
                     LALTFRDEGIGISPEDQQHLFTSFFRGGNASNIEGTGLGLHIVKRYLDLLGGSIHLES
                     MLGQGTTISVSLPEGGLNGK"
     gene            complement(1226..1930)
                     /locus_tag="VKU83_12375"
     CDS             complement(1226..1930)
                     /locus_tag="VKU83_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLI94407.1"
                     /translation="MGASLVFAFSIGLISSLHCAAMCGPLVLAAPFQVLPPRLRWLGT
                     GLYHSGRIGIYAAGGLVFGLAGRAIYLAGQQHLLSILLGTAILAQLLFRRYSGRGWMP
                     RWLSSCYLTLQRVMRRWWASPSLAKFLLLGVGNGLLPCSMVYLAIAGALTMNGAAKAM
                     AFMAMFGIGTLPLLLGVQVAGRISGLGTRQRFRRLVPLLTAAIACLLICRGLNLGIPF
                     ISPVLADKPGTPVTCH"
     gene            complement(1937..2374)
                     /locus_tag="VKU83_12380"
     CDS             complement(1937..2374)
                     /locus_tag="VKU83_12380"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HLI94408.1"
                     /translation="MNWGNKIILVFVLFAGMISYMVYRCIKLPVDLVSDEYYKDELAY
                     QQVIDGTKRANALSGRVELAHAGSGFILTMPAEMRHRPVLGNIFFYCPADASRDRTIP
                     LLPDAMGAQWIPANAIPKGRYRLKISWETQGVSYYAEQNLYYQ"
     gene            complement(2393..>3595)
                     /gene="ccoG"
                     /locus_tag="VKU83_12385"
     CDS             complement(2393..>3595)
                     /gene="ccoG"
                     /locus_tag="VKU83_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HLI94409.1"
                     /translation="EARFIIFGKIFWPQDFFIFGLTMVTFVVFVILFTAAFGRLFCGW
                     VCPQTIFMEMVFRKIEYLIEGDAAEQRLRRQGRRTVGGQARIALKHAVFFLISFVIAN
                     FFLAYIISIRSLWAIMTEPVAAHLAGLASLLLFAGVFYAVYAYFREQACTVVCPYGRL
                     QGVLLDRNSMIVAYDYRRGEPRGKFAKNKPETLGDCLDCNQCVKVCPTGIDIRNGTQM
                     ECIGCTACIDACNFMMEKTGRAPNLIRYASENGISVHEPLRYTLRMKLYTGLLSVLVV
                     LVALLLLSRKEVDATVMRTPGMLYQERGTDSISNLYTIKVANKTIRAVPLQLRLEDRE
                     GNIQVIGGRTITIRREDEGAGNFFVVLPRRSLMERKTRIRLGLYDGKDKIDDITTNFL
                     GPVTGDAD"
BASE COUNT          851 a          997 c         1030 g          717 t
ORIGIN      
        1 ggggagcagc accaggttaa cgcccttttt gaacatgcca ccgaaggaat cgtcctgacg
       61 gacgacaagg gcaggatggt gctcctgaac cctgccgcgc tgcaactttt tgaatataac
      121 cgtgaggagt tgcttggtca gccgatcgag atgctgatcc ctaagcgatt cagcccacgg
      181 cataccgact atcggcatgg tttttatcaa gcacccggca accggacgat gggccacggc
      241 cgggacctgt ttgcccggaa gaaagatgga agcgaatttc ccgtcgaagt tagcctcagc
      301 tattaccggc agaaagggag tatgtacgtc attgcattcg tcgtggacat cactgcccgt
      361 aaacaaagcg aaaaagagct tctggaccga aagtcgcagt tggaaaagat catggaggac
      421 gtgaggcggt tgaatgccga gctggaaggg aaggtggagg aacgaacgct cgtccttcgg
      481 gaggcattgc aggagctcga gaagtcccag caggagctgc atgaagcgct ggacaaggag
      541 cggcaattaa atgagatcaa atcgcgcttc gtttccatgg cctcgcacga gttccggacg
      601 ccgctgagta ccgtattgtc atccgccgcg ctgctctcaa aatacccgac aacggatcaa
      661 caagtcgtcc gcgagcgaca tatccggaag ataaaggatt cggttaaaca gctcaacgac
      721 ttactcgggg attttctctc actggggaag ctggaggaag gaaaagtcat agcggatccc
      781 tcgcctttcg acgtgaagga tctgctggac gagacagttg aggaaatgcg gtcgctgtcg
      841 aaggacggac agaacattca ctgtaatttt accggtgacc gcagcttcct gacagacaaa
      901 aggcttttga agaatatcgt gatcaacctg cttggcaatg ccatgaagtt ttcgccggat
      961 ggcgcatcca tctggctgga agtgaagcgg cgtccaggag tacttgcact cacgttccgg
     1021 gatgagggga ttggcatctc gcccgaagat cagcagcatt tgttcaccag ttttttccgc
     1081 ggcggcaacg cgagcaatat tgaagggacc ggccttgggt tgcatattgt caagcgatac
     1141 ctcgacttgc tcggcggatc gatccatttg gagagtatgc ttggtcaggg cacgaccatc
     1201 agtgtctcgc ttccggaagg cggcctcaat ggcaagtgac aggagtaccg ggcttatcgg
     1261 ccaggaccgg gctgataaaa gggatgccga ggttgagacc gcggcagatc agcaaacagg
     1321 caatcgccgc cgtcaggagg ggcaccaggc gccggaatcg ttgtcgggtt cccaggccgg
     1381 atatccttcc cgcaacctgt acgcccagca ataagggcaa agtgccgatc ccaaacatcg
     1441 ccataaaggc catggctttc gccgcgccat tcatggtcag tgcccccgcg atcgccaggt
     1501 acaccatgct gcagggcagc agtccattgc ccactcccag cagcaggaat ttggccaggg
     1561 agggggaagc ccaccagcgg cgcatcaccc tttgcagcgt cagataacag gaagaaagcc
     1621 agcggggcat ccatccgcgc ccgctatagc gccggaacag caattgggcc aggatggccg
     1681 tacccagcaa gatcgagagc aggtgctgct ggccggcaag gtagattgcc cggcccgcca
     1741 gtccgaatac caggccgccg gcggcataga tcccgatgcg gccgctgtgg tataagccgg
     1801 ttcccagcca acgaagtcgc ggcggcaaca cctgaaacgg cgcggcaagc acgagcggtc
     1861 cgcacatggc ggcgcagtgc agtgaactga tcagtccgat cgaaaacgcg aaaaccagcg
     1921 atgcccccat acacgtttat tgataataca ggttctgttc ggcgtagtag ctgacccctt
     1981 gtgtctccca gcttatcttg agacgatatc ggcctttagg gatggcgtta gcggggatcc
     2041 actgcgcgcc catggcatcc ggcaggagcg gaatggtcct gtcccttgag gcgtcggccg
     2101 gacagtaaaa gaaaatattg cccagcacgg gacggtgccg catttcagcc ggcatcgtca
     2161 ggatgaaacc gctacccgca tgggccagct ccacgcggcc ggacaaggca ttcgcccgtt
     2221 tcgtgccgtc gatcacctgc tgataggcca gctcatcctt ataatactca tcggatacga
     2281 ggtcgacggg caatttgatg caacgataga ccatgtaaga gatcatcccg gcaaaaagga
     2341 cgaataccag tataattttg tttccccagt tcatgagatg ctgtttaatg gtttaatcgg
     2401 cgtcccccgt gacaggtccg aggaagttgg tggtgatatc atcgatttta tcctttccat
     2461 cgtataatcc cagcctgatc cgcgtctttc gttccatgag gctccgccgc ggaaggacaa
     2521 cgaaaaaatt gcccgcgcct tcgtcttccc tgcgaatggt gatcgtccgc ccgccgatca
     2581 cctgaatatt tccttccctg tcttcaaggc gcagttgcag cggcaccgcg cggatcgttt
     2641 tgttggctac cttgatcgta tacaggttgc tgatcgaatc cgtgccccgc tcctggtata
     2701 gcattccggg agtccgcata acggtggcat ctacctcttt cctgctcagg agcagcaggg
     2761 caaccaggac aaccaacacc gaaagcaggc cggtatataa tttcatgcga agggtatatc
     2821 gaagcggctc atggacggag atgccgtttt ccgaggcata ccggatcagg ttgggcgcgc
     2881 ggcccgtctt ctccatcatg aagttgcacg cgtctatgca ggcagtgcag ccaatgcatt
     2941 ccatttgtgt gccgtttcgg atatcaatcc cggtgggaca gaccttgacg cattgattgc
     3001 agtccaggca gtcgccgagt gtctcaggct tgtttttcgc gaatttgcct ctgggctcgc
     3061 cgcgccggta gtcataagca acgatcattg aattccggtc caacaggaca ccttgcaacc
     3121 ggccgtaggg acagacgacc gtgcacgcct gttccctgaa ataagcatat acggcataaa
     3181 aaacgcctgc gaagaggagc aacgacgcga ggccggcaag atgcgccgcc accggttcgg
     3241 tcatgatggc ccacagcgag cggatgctga tgatgtaggc gaggaaaaaa ttggcaatca
     3301 cgaaggagat caggaaaaag acggcatgct tcagcgcgat gcgcgcctgc cctcccacag
     3361 ttcgcctgcc ctggcgcctc aggcgctgtt ctgccgcgtc tccttcgatc aggtattcga
     3421 tctttctgaa gaccatctcc atgaatatcg tttgcggaca aacccagcca cagaagaggc
     3481 ggccgaaggc cgccgtaaag aggatcacaa acacgacaaa ggtcaccatc gtcagcccga
     3541 agatgaaaaa gtcctgcggc cagaagatct tcccgaagat gatgaatcgc gcctc
//
LOCUS       DATGKE010000729         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_159481, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000729 DATGKE010000000
VERSION     DATGKE010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159481"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VKU83_12390"
     CDS             complement(<1..1680)
                     /locus_tag="VKU83_12390"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HLI94410.1"
                     /translation="MRTSLAAFAVLLATTCSAQVSDSADIAARIDRHEFDPSTLLWYQ
                     APAATWEAALPVGNGRLGAMVFGREEEERIQLNEATYWTGGPYSTVVKGGYKELPEIQ
                     KLVFEGKYLQAHTLFGRYLMGYPVEQQKYQCLGNLHLFFVHSSGISHYKRWLDLETGV
                     TGVQYTADGVSFRREVFVSAPDQVIVIRLTADKPKSISFSIRLRGVRNQTHSNYATDY
                     FRMDGWGQDGLVLTGKSADYMGVPGKLRYEARLKAVPEGGSMKVEDDLLTISGADAVT
                     LYVVAATNFVNYHDVSADAHRRVEGYLAALAARPYETIREAHLTDYRRLFCRVALHLP
                     INNVSFEPTDSRKAKVSDPSLAALAYQFGRYVLISSSRPGSQPANLQGIWNDNPNPMW
                     DSKYTTNINTEMNYWPAESANLSECAGPLFQMIADLTDQGAEVAREHYGCRGWVFHQN
                     TDLWRVAAPMDGPTWGTFTVGGAWLCTHLWEHYAYTGDTAFLNQMYPVIKGSVRFFLD
                     FLVPTPDGRWLVTNPSTSPENFPDRPGNGPYFDEVTGSFLPGTSICAGSSID"
     gene            1743..1964
                     /locus_tag="VKU83_12395"
     CDS             1743..1964
                     /locus_tag="VKU83_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94411.1"
                     /translation="MQRTLGVILMVIGLIMLIWTGFSYTQREKVVDAGPVHISADKHK
                     SVNWPPYAGGIILVVGVVLVATGPGRKSV"
     gene            complement(1982..2782)
                     /locus_tag="VKU83_12400"
     CDS             complement(1982..2782)
                     /locus_tag="VKU83_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94412.1"
                     /translation="MFSSIRFAGHGCRLLVFAASFAVNLFSGRAWAAGPSDSRLDRMP
                     TDLETELALSALPANLRAGATVYLLDPAKGFYPACRGTNGFVCFVSRTEWEWGEFRND
                     LFAPMGFDAEGARTNFIPYLDVAAMRASGKYTARQIKDSVMLRIRKGIYKAPARSGIC
                     YMLAPIMRVYTGMPGNENVMTMSMPHYMFYAAHVKDSDIGTDPHSNDGPWLVNPGNTV
                     LGAGNGIWGYIIVPARESVAAKIRQSGQSLVQRLAAYSPYFKLDAAMK"
BASE COUNT          664 a          842 c          862 g          619 t
ORIGIN      
        1 atcgatcgaa gatccggcac agatgctggt gccgggcagg aaagaccctg tgacttcgtc
       61 gaagtatggt ccgtttccgg gccggtcggg gaagttctcg ggggacgtgg aaggattcgt
      121 gaccagccac cggccgtccg gtgtcggcac caggaaatcc aggaagaatc ggacggaacc
      181 cttgatgacc ggatacattt ggttcaggaa tgccgtatcg ccggtataag cgtaatgttc
      241 ccataaatgg gtgcaaagcc aggcgccacc gaccgtgaac gtgccccagg tcgggccgtc
      301 catcggcgcc gcgacccgcc agagatccgt attctggtgg aacacccatc cgcggcaacc
      361 ataatgttcc cttgcaacct ccgccccctg atcggtcaaa tcagcgatca tttggaacaa
      421 gggcccggca cattccgaaa ggttggcaga ctccgccggc cagtaattca tctcggtatt
      481 gatatttgtc gtgtatttgg aatcccacat cgggttcgga ttatcattcc agataccttg
      541 cagattggcg ggttgcgatc cgggccgaga agacgatatc aacacgtaac ggccgaattg
      601 ataggccaac gcagccaggc ttggatcaga gaccttcgcc tttcgcgaat cggtcggctc
      661 gaaggaaaca ttgttaatgg gcaggtggag cgctacccgg cagaacaggc gccggtaatc
      721 ggtgagatgc gcctcgcgga tagtctcgta tggtctggcg gccagcgcgg ccaggtagcc
      781 ttccacccgc cggtgcgcgt ccgcgctgac gtcgtggtag ttgacaaagt tggtagcggc
      841 gaccacatac agcgtcacgg catctgcccc gctgatcgtc agcagatcgt cctcgacctt
      901 catcgatccg ccctccggca cggccttcag gcgggcttca tatcgcaatt tgcccggaac
      961 ccccatatag tcggccgact tcccggtcag gaccagacca tcctgtcccc agccgtccat
     1021 ccggaagtag tcggtagcgt agttggaatg ggtttgattg cggactcccc ttaagcggat
     1081 gctgaaagaa atgcttttag gcttgtcggc cgtcagccgg ataacgatga cctgatcggg
     1141 ggccgaaaca aaaacctccc gccggaaact gacgccatcc gccgtatact gcacccccgt
     1201 aacgccggtc tcgaggtcca accaccgctt gtaatgactg ataccggatg aatggacaaa
     1261 gaacaaatgg agatttccca ggcattgata tttctgctgt tcgaccgggt agcccatcag
     1321 gtaacggccg aaaagcgtgt gggcttgcag atatttgcct tcaaatacta atttttgaat
     1381 ctcgggaagc tccttgtagc cgcccttcac gacggtggaa taaggaccgc cggtccaata
     1441 ggttgcctcg ttgagctgga ttcgttcttc ttcttccctg ccaaacacca tggcgccgag
     1501 ccggccgttt ccgacgggca gcgccgcttc ccatgtggcg gcgggagcct ggtaccaaag
     1561 gagcgtggaa ggatcgaatt cgtggcggtc gatccgggcg gcaatatccg cggaatcgct
     1621 gacctgtgcg gaacaggtcg tggcaagcag aacggcaaag gcggcaaggg aagttcgcat
     1681 gaacctaaga taagaaaaat tgccgccttc cgtcgtggca tggtttttta acctcaagga
     1741 ttatgcaaag aacactgggg gtcatattga tggtgatcgg gctcattatg ctcatttgga
     1801 caggcttttc ctatacgcaa agagagaagg tcgtggatgc cggcccggtc cacatttctg
     1861 ctgacaagca taaatcggtg aactggccgc cctatgcggg aggcattatc ctggtagtgg
     1921 gcgtcgtgct ggtcgcgacc ggtccggggc gcaaatcggt ttaggcgctg agccaacaac
     1981 ctcatttcat agcggcgtca agcttgaaat acggactgta ggccgccaaa cgctgaacaa
     2041 ggctttgccc ggactgccgg atcttcgccg cgaccgactc gcgtgcaggc acgatgatat
     2101 acccccagat cccgtttccc gcgccaagaa cggtattacc tggattcacg agccaggggc
     2161 cgtcattcga atgagggtcg gtgccgatat ccgaatcctt cacatgcgcc gcataaaaca
     2221 tatagtgcgg catactcatc gtcatcacat tctcgttgcc cggcattcct gtatacaccc
     2281 gcatgatcgg agcaagcatg tagcagatac ccgacctggc aggcgcttta taaatcccct
     2341 tccggatccg cagcatcacc gagtctttga tctggcgggc ggtgtatttt cccgacgccc
     2401 gcatggccgc tacgtcgagg taagggatga agttggtgcg ggcgccctcc gcgtcaaatc
     2461 ccatcggcgc aaaaaggtca ttgcggaatt cgccccattc ccactcggtc cgggatacaa
     2521 aacaaacgaa accattggta ccgcggcacg cgggataaaa gcctttcgcc ggatccagca
     2581 gataaacggt agctccggcg cgcaagttgg ccggcagcgc gctcagggcc aactcggtct
     2641 ccaaatcggt cggcattcta tcgagccggg agtctgatgg tccggcggcc caggcgcggc
     2701 ccgaaaaaag gttgacagcg aaagacgcgg cgaaaacgag tagccggcaa ccgtggccgg
     2761 caaagcggat ggaagaaaac ataaatagtc gattaaatca ggtaagtcag tttggtaccg
     2821 aaaattacgg ctgttcatct tctgcaacga ggggggaatg cgtcataccg gggggcaatt
     2881 tgggacattc ccggcaggat tccgctatct ttggatccat gagaaaattc tgcctgcttt
     2941 gcatcctcgt tctgactgcc tttcagcgcc ggcccctggt ctgggtt
//
LOCUS       DATGKE010000730         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1300441, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000730 DATGKE010000000
VERSION     DATGKE010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300441"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VKU83_12405"
     CDS             complement(<1..698)
                     /locus_tag="VKU83_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94413.1"
                     /translation="MGTLIRLSRKAYGLCIIAMGIQQLAYGKVSGDFLPEVFATYRGY
                     PLIAYSWGALFTLWGIALFFERKGYEVSLISATVFMLLLVGIYIPYCLLAGPFGLLDW
                     SGPIEESAFVGASLIVASSYPPYTGKSAFIRSLSRLIPLGGLFFAIMLICYGTDHFVY
                     TSFVSNMVPAWIPSHFFWTYFAGVALISAGIAIALRIGVRVAASLLALMIFLWFLIVH
                     IPTAIRDPAGNGGL"
     gene            complement(806..1471)
                     /locus_tag="VKU83_12410"
     CDS             complement(806..1471)
                     /locus_tag="VKU83_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLI94414.1"
                     /translation="MIIPTVIDSATRGAFDIYSLLLKERIVYLGTAIDENVANLVVAQ
                     LLYLDSENHNPVMLYLNSPGGIVYGGFAIYDTMRKLKSPVTTVAMGFCGSMSTVLLAA
                     GRKGQRYALPHATIHMHPTGGGAKGYTEDVRIAYKEQERLQNQIFYLIGKFSGHTREE
                     IEEMFRRDRFMNSVEALQYGLIDEVLGDTDDLISIDDASAMIRFQGPGKERGSIGFRQ
                     SPG"
     gene            complement(1449..1730)
                     /locus_tag="VKU83_12415"
     CDS             complement(1449..1730)
                     /locus_tag="VKU83_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94415.1"
                     /translation="MRNELNEIQLIDRYLFRKLDQAECRAFEASLLVDESLAAKVEAQ
                     RIAHRLIRLYGQNEDRRRFNCIYRRLQCEESFSRRLKHIFPRHDHSDRD"
     gene            complement(1720..2232)
                     /locus_tag="VKU83_12420"
     CDS             complement(1720..2232)
                     /locus_tag="VKU83_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94416.1"
                     /translation="MTDNAEDPIVSLYRDAFPDFARMVGKMGGTLEQAKDNFHDALLI
                     YLQKEKAGTLHLNNSPKAYVIGTAKICWLRAKKSGRTAALEGLEISDQENEAGNNAVE
                     RENRLLESLVKSGRKCLELLKAFYYDQVSMQDIAGRFGFSGGRSATVQKFKCLEKVRK
                     EMTSSRRYAQ"
BASE COUNT          619 a          759 c          640 g          582 t
ORIGIN      
        1 tcgagtccgc cgttgccggc cgggtcccgt atcgccgtgg ggatatgcac gatcaggaac
       61 caaagaaaga tcatgagcgc cagcaggctc gcggccaccc gcacaccgat ccgcagcgcg
      121 atcgcgatgc cggcgcttat cagcgcgaca ccggcgaaat acgtccagaa aaaatggctc
      181 gggatccagg cagggaccat gttggaaaca aatgaagtat agacgaaatg gtcggtgccg
      241 taacaaatca gcataatagc gaagaacaac ccgccgaggg gaatcagcct tgacagcgat
      301 cggataaaag ccgacttgcc ggtgtacggc ggataagaag atgccacgat cagtgaagca
      361 cccacaaatg ccgactcttc gattggtccc gaccaatcga gaagtccgaa ggggcccgcc
      421 aacaaacaat acgggatata gatgcccacc aggagcaaca taaaaaccgt cgccgaaata
      481 agcgaaacct cgtatccctt cctttcaaaa aataacgcaa tcccccaaag cgtaaaaagg
      541 gcgccccagg agtaagcgat cagcggatag ccccggtaag ttgcaaatac ttccggcaaa
      601 aagtcaccgc ttaccttgcc ataggccagc tgttgtattc ccattgcaat gatgcaaagg
      661 ccgtatgcct tccggctcaa ccgaattaag gttcccatag ccgcttttcc gtaaatgtat
      721 tcataatatt gacggcaacc aaccacgttc cgacaagcgc ccggcaacaa taatccgcga
      781 cgccttcatg gcgaacacgc ctcgcctagc cggggctctg cctgaatccg atacttcccc
      841 gctctttccc gggcccctga aaacggatca tggcggatgc atcgtcaatc gagatcaggt
      901 cgtcggtgtc ccccaacact tcatcgatca gtccatactg aagggcttcc acgctgttca
      961 tgaaccggtc gcgccggaac atctcttcga tctcttcgcg cgtatgaccg gaaaactttc
     1021 ctatcagata gaatatttga ttctgcagcc gctcctgctc cttatacgcg atccgcacgt
     1081 cttccgtata tccctttgca cctccgccgg tggggtgcat atggatggtc gcatgcggga
     1141 gggcataccg ctgacctttg cgtccggctg ccagcaatac ggtactcata ctgccacaga
     1201 agcccatggc caccgtcgtc accggcgact tcagcttccg catcgtgtca taaatggcaa
     1261 atcctccata gacgataccg ccggggctgt tcagatagag catgaccggg ttgtgatttt
     1321 cgctgtcgag atacagaagc tgggcgacga ccaggttagc gacattttcg tcgatcgccg
     1381 ttcccaggta tacgatgcgt tctttcagca gcaggctgta aatgtcaaat gcgccccggg
     1441 tggcgctgtc aatcacggtc ggaatgatca tgtcggggaa agatgtgttt tagccggcgc
     1501 gaaaaggatt cctcgcactg cagccgccgg taaatgcagt tgaaccgtct ccggtcctcg
     1561 ttctgcccgt aaagccggat cagccggtgg gcgatgcgct gcgcctcgac cttcgccgcg
     1621 agggactcgt cgaccaagag gctggcctca aatgcccggc actcggcctg atcgagcttc
     1681 cggaaaagat aacgatcgat cagttggatc tcgttcaatt cattgcgcat atctccggct
     1741 tgatgtcatt tccttgcgga ccttctccag gcatttgaat ttctgcacgg tcgcagacct
     1801 gccgccgcta aagccaaacc ggccggcaat atcctgcatg gaaacctggt cgtaataaaa
     1861 tgcttttagc agctccaggc atttcctgcc gctctttacg agcgactcaa gcaacctgtt
     1921 ttccctttcg accgcattgt tcccggcttc gttttcctga tcagaaattt ccagtccctc
     1981 aagtgccgcc gtccgcccgc ttttcttcgc ccgcagccag cagatctttg cggtgccgat
     2041 cacataagcc tttggggagt tattcaggtg aagcgttccg gccttttcct tctgaaggta
     2101 gatcagcaac gcatcgtgaa aattatcttt agcctgttcc agcgttccgc ccattttgcc
     2161 aaccatccgg gcaaaatcag ggaaggcgtc ccggtaaagg ctgacgatcg ggtcttctgc
     2221 gttgtcggtc atggcctgct ctttaccttt aagtgtctga aatccggcaa agatcaccct
     2281 aaaaatacga ttttcggtgc ccgtcaccgg acagtagtat atcatgaacg aacaaagact
     2341 caaaaacgaa atatataaag ccatgcgaat caagcagcac aatcatggca cgactttccg
     2401 ttcattcggg aatccaaact gctatccatg atccggagtt attttaccat tgcctggcgc
     2461 ggtctcctcc gtaacaaacg gtcatcggtc atcaacatct tggggcttac gcttggcatc
     2521 acctgcagcc tgcttattct tttgtgggtg cgtgacgaac gtagcgtcga ttccttccac
     2581 aagaacgcgc gcttattata
//
LOCUS       DATGKE010000731         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_799060, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000731 DATGKE010000000
VERSION     DATGKE010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799060"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VKU83_12425"
     CDS             complement(<1..234)
                     /locus_tag="VKU83_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94417.1"
                     /translation="MKRINFKNYKISVPASIVLMALLVACGKNFLVKQPIGPITPAVL
                     GTYSGVQGLLIGAYAAVIGEGTNNSGWGSAADNW"
     gene            complement(260..>3416)
                     /locus_tag="VKU83_12430"
     CDS             complement(260..>3416)
                     /locus_tag="VKU83_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLI94418.1"
                     /translation="SLFAQQTVTGKVIGSTDKQPVPFATVQVKGERGATQTGTDGSFS
                     IRLTKSSGTLVISAVGFEALQVPVSAGNAALGVITLTASTSTLNDIVVTGYTAQRKKD
                     ITGAVAVVDMKDMKSVPGTSTEALLQGQAAGVQVVNSGMPGGGSNIRVRGVTSIGNVD
                     PLVIIDGVQGSMHDLDMNDVESIQILKDAGAVAIYGIQGSNGVVIITTKRGRGKSSIS
                     YDGFYGTQQPVKGFDLANSQTYANVLWLQYYNAGITPGNEWFGKGGPTYAPPVLPDYL
                     SPTPYSDANGTPNPNYAAMQNPATYDIVSNQITKTDKSGNNWFKDIFKAAPWQQHTVA
                     ASGGGDKSSYYISLSYLDQQGTLIDTYLKRYAVRANTTFNVKDHVRLGENAYVFFKEN
                     PQITNQNEGNAISYSYRTPPLIPVYDIMGNYAGTHTFTINNSDNPVADQNNQANNKGN
                     DWEINGNFFADVDFLQHFTAHSSVGGSVDNYYYYYFGYTPYMNAEGSTAANNFTEGAG
                     YNSTLLWTNTLTYNQTFGDHSIKALVGSEAKEYYTRGLSGERGVYAFTTPSYWTLNNG
                     TASTQTNTNINGPYAESLFSLFGRVDYAYQDKYLVSGTLRRDGSSKFAQGHQYNNFPS
                     VSVGWRISKEDFMKDITWLQDLKLRGSWGKSGNLSNVPSTNPYTLFSSSPQNSNYDIN
                     GTSTGSTLGFYNSQIGNQFTSWENDIMSDVGLDATLGRFDVTLDWFKKTISGELFQKQ
                     LAQTVGGGAPPYVNFGNLTNTGFDGSIGYHQTVNKDLSFNVLATFTHYVSKVATLPPG
                     IQYYDNFTNAGSNRIGAFTRVQPGHAVGEFFGYKVIGYFRDTSDVAKSPTQPGAAPGA
                     FKYQDVNNSGDINDSDRTWIGNPNPKLMYGLNLGLSYKNWDFSAFFYGVYGNDIFNYV
                     KYWTAFPQVFEGNVQSDLIQNSWSPTNLNPKYPQITNVGSFSNADVVNSWYVEKGSYF
                     RLKSLNIGYSLSPATLQKVGINKLRIYVQGANLFTITKYSGLDPELQASNLYDQTNFG
                     VDLGNYPSNQKTFIVGVNLNF"
BASE COUNT          752 a          886 c          914 g          864 t
ORIGIN      
        1 ccaattgtcc gctgcggagc cccaaccgga gttgttagtc ccttcaccga tcaccgctgc
       61 ataggcgcca atcagcaagc cctgaacgcc cgaataggtc cccaacacag ctggcgtaat
      121 tggcccgata ggctgtttaa caagaaagtt cttcccacag gccaccagca aagccatcag
      181 gacgatactg gcgggtacgg aaatcttgta gttcttaaaa tttattcttt tcataacgtg
      241 atagttattt cggttttctt tagaaattaa ggtttacgcc aacaataaag gtcttctggt
      301 tcgaaggata gttacccagg tcaacgccga agttcgtctg gtcgtagagg ttggatgctt
      361 gcagctccgg atccaggccc gagtatttgg tgatcgtaaa caggttagcg ccttgcacat
      421 agatcctcaa cttgttgatg ccgacctttt gcagggtagc cgggctaagc gaatacccga
      481 tattcagcga tttcaaccgg aagtacgagc ctttctctac gtaccatgag ttcacgacgt
      541 ctgcgttcga gaaggaaccg acatttgtga tctgcggata cttcggattc aggttggtcg
      601 gcgaccagga attctggatc aggtccgact gcacgtttcc ttcgaatact tgggggaacg
      661 cggtccagta cttgacatag ttgaaaatat cgttgccgta taccccataa aagaatgccg
      721 agaaatccca gttcttgtag ctaagcccga ggttcaatcc atacatcagc ttaggattgg
      781 gattgccgat ccaggtccgg tcgctgtcgt tgatatcgcc gctgttatta acatcctgat
      841 atttgaacgc tccgggcgct gctcccggtt gcgtgggcga tttcgcaacg tccgacgtat
      901 cgcggaaata cccgatgacc ttgtacccga agaactcacc cacggcgtgt cctggttgaa
      961 cccgggtgaa tgccccgatc cggttggaac ctgcgttggt gaaattgtca tagtactgga
     1021 tccccggggg cagtgttgcc accttgctga catagtgcgt aaaggtcgcc aggacattaa
     1081 aacttaaatc cttgttgacg gtctgatggt agccgatcga cccatcgaaa cccgtgtttg
     1141 tcaggttccc gaagttgaca tatggtggcg ctccgccgcc aacggtttgt gcaagctgct
     1201 tctggaacaa ttcaccgctg atggttttct tgaaccagtc taacgttaca tcgaagcgac
     1261 cgagggtggc gtccagaccg acatccgaca taatgtcgtt ttcccagcta gtaaattggt
     1321 tgccaatctg gctgttgtag aaacccaatg ttgagccggt ggacgtcccg tttatgtcat
     1381 agttggagtt ctgcggtgaa gaagagaaca aggtgtaggg attcgtgctc ggaacgttac
     1441 tcaggtttcc ggacttaccc cagctgccgc ggagcttcag gtcctgcagc caggtgatat
     1501 ccttcatgaa atcttctttc gagatccgcc agccgaccga cacagaaggg aagttgttgt
     1561 actgatggcc ttgtgcgaat ttcgaagagc cgtcccgccg cagggtaccg cttaccaggt
     1621 atttgtcctg gtaggcataa tccacgcgac cgaagagcga aaacagcgac tcggcatatg
     1681 gcccgttgat attggtgttc gtctgagtag aagccgtacc attattcaac gtccagtacg
     1741 aaggcgtggt aaacgcgtaa acgccgcgtt cccctgacag gccacgcgta tagtattcct
     1801 ttgcctcaga tccgaccagc gccttgatcg agtggtcacc gaaggtctgg ttataggtca
     1861 gggtatttgt ccaaagcagc gtgctattgt acccggctcc ttccgtaaag ttattggccg
     1921 ccgtgcttcc ctccgcgttc atatatggag tgtagccaaa ataatagtaa taataattgt
     1981 ccactgaacc gccgacgctc gaatgggctg tgaagtgctg caggaagtcg acatcggcaa
     2041 agaagtttcc gttgatctcc cagtcattcc ctttgttgtt ggcctggttg ttctgatcgg
     2101 caactgggtt atcggagttg ttgatcgtaa aagtgtgcgt gccggcataa tttcccatga
     2161 tatcgtaaac cgggatcagc gggggggtcc ggtaagaata ggagatcgcg ttgccttcgt
     2221 tctggttggt gatctgcgga ttttccttga aaaaaacgta agcattctct cccagcctga
     2281 cgtgatcctt cacattgaag gtagtattgg cgcggacagc atatctcttc aaatacgtat
     2341 cgatcagcgt gccctgttga tccaggtagc tgagcgaaat atagtaggag ctcttgtcac
     2401 ctccgccgct ggccgctacg gtgtgctgct gccagggggc ggccttgaag atatccttga
     2461 accagttgtt gccggattta tctgttttcg taatctggtt gcttacaata tcatacgttg
     2521 cagggttctg cattgcagca taattcggat tcggcgtacc gttggcatcc gagtatggcg
     2581 tgggactaag gtagtccgga agcaccggcg gcgcatacgt gggtccccct ttgccaaacc
     2641 actcgttgcc gggtgtgatt ccggcattgt agtactgaag ccaaagaacg tttgcatatg
     2701 tctggctgtt ggccaggtcg aatcccttta ccggctgctg ggtaccataa aaaccatcat
     2761 agctgatcga agactttccc cgaccgcgtt tggtggtgat gatcacaacc ccattggagc
     2821 cctggatacc atagatcgcg accgcaccgg catccttgag gatctggatg gactcgacgt
     2881 cgttcatgtc aagatcatgc atgctaccct gcacgccgtc gatgatgacc agcggatcga
     2941 cgttgccgat agaggttaca ccccggacac gaatattgct tcccccgccc ggcatccccg
     3001 agttgacaac ctggacaccg gcagcctgac cctggagcag ggcctctgtg ctggttccgg
     3061 gcacgctctt catatccttc atgtccacca cggcaacagc ccccgtgata tctttcttcc
     3121 tttgggcggt gtaacccgta acgacgatgt cgttcagggt ggacgttgaa gcagtgaggg
     3181 tgatcacgcc aagtgcggcg tttccggcgc tgacgggcac ctgcagggcc tcaaagccta
     3241 ccgcggagat gaccagggtg ccggagcttt tcgtgagtct gatggaaaaa ctaccatcag
     3301 taccagtctg ggtagcgcct ctttccccct tgacctgcac ggtagcaaac ggaaccggct
     3361 gtttatccgt gcttccgatg actttaccag tgactgtttg ttgggcaaaa aggcta
//
LOCUS       DATGKE010000732         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1506143, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000732 DATGKE010000000
VERSION     DATGKE010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506143"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VKU83_12435"
     CDS             complement(<1..908)
                     /locus_tag="VKU83_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI94419.1"
                     /translation="MSNSVIKVDSLSKRFLIAHEAPERYTTLRDTISRGAKRLLPGRH
                     ADKRQKGSKEEFWALKDVDFEIQPGERVGIIGRNGAGKSTLLKVLSRITEPTRGKVSI
                     RGRVASLLEVGTGFHPELSGRENVFLNGAILGMSRVEIRKKFDEIVAFAEVEKFLDTP
                     VKRYSSGMYVRLAFAVAAHLEPEILVVDEVLAVGDAQFQRKCLGKMEEVSSKDGRTVL
                     FVSHNMGAIKSLCTSAIILHKGRKILQGDVFESLAKYMSFDQTQLNQDTRYRREGVPA
                     GKIMFIREAEFVDDQSRDRFSIQVNLV"
     gene            complement(918..1745)
                     /locus_tag="VKU83_12440"
     CDS             complement(918..1745)
                     /locus_tag="VKU83_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLI94420.1"
                     /translation="MEELIIEPGGVEKNYWRDLWRYRELFYILSWRDIKVRYKQTVVG
                     AAWGVIRPLLTMIIFTFIFGRIANLAVKNGPPYPIVVFAGLLPWQFFSAALTEASNSM
                     IGNVNLISKVYFPRMIVPAASVITSLVDLGISFGLLLLMMLFYRFVPPLQMLFLPLFA
                     ALAFLISFGIGLFVTALNVKFRDFRYIIPFVVQFGIYITPVGYSSAVIAQKYSDTARF
                     WFSLNPMVGVIDGFRYCIVGEPMYWPGFFLSLGVMLLFLWLGISYFRKTEKSFADLI"
     gene            complement(1780..>3948)
                     /locus_tag="VKU83_12445"
     CDS             complement(1780..>3948)
                     /locus_tag="VKU83_12445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HLI94421.1"
                     /translation="ELFMSQGVSNLSNEIEILKSKPVLQRVARDLNLQIYYYNKGNVK
                     TSFMYPDPPFFLEPLSIVDSASGFNCMITLLGNGKFLLDENKTKLEFGEPFKVGNNLC
                     RLTRNPAYNENSFVNAKFSTGWQPFKYAAENLAGGLSVSQANDQSTILTLAFEGENAN
                     LGNDVLNTLMSVYDSLIIEDKSRINANTLNFINTELYTLNDTLKGAQGGLRNYMVENQ
                     VFDLDAQSKNYLDNLKDVIKDRDEQEVRLNIIDWLLKYIADKKHAYDLVPTDLGVQEP
                     ALVPMIAEYNRLQLERQATLRTTPETNSLVVGMEVSLEGIRKEIYQALLNVKESYKIA
                     GSKLESHEQELQNGIRSMPGKSIQLLNRQRQQRILEELYSLLLQKKLETQIASASTVS
                     NSKVVEPAAGAGTEISPDRKKIYTLYILLGLLIPCAVIALIEVLRDKVTGRADVEKNT
                     AAPILGEIGHSNYKNTLVVTKNSRLFISEQFRIIRTNLQYVIGKKEKPVIMVTSSFSG
                     EGKSFISINIGAVMALADRKTVIMEFDIRKPKIVSGLELRRKMGITNYLIGSAKFEEL
                     VVKVDEVDNLYVIPCGPIPPNPSELLLDPRLDELMAEVHKRFDVIIMDTAPVGLVSDA
                     VNLVRFADCTLYIVRQAYTFRRQINAIDELYTQKKLPKISLLLNDVKMEGGYYGGYNG
                     GGYYGGYGYGGDSGYFENERGMGNGSIFRRVRAQLERWFG"
BASE COUNT          931 a         1097 c          939 g          981 t
ORIGIN      
        1 acaaggttta cctggatgga aaaacgatcc ctgctctgat cgtcgacaaa ttcggcctcc
       61 ctgataaaca ttattttccc cgccggcact ccctcgcgcc ggtatcttgt atcctggtta
      121 agctgcgttt gatcgaaaga catgtatttc gccagcgatt cgaagacatc gccctgcaat
      181 attttcctgc ccttgtgcag gataatggcg gaggtgcaaa gggatttgat cgcgcccata
      241 ttgtggctga cgaacagcac ggtccttccg tccttgctgc tgacttcttc catcttaccc
      301 aggcatttac gctggaattg ggcatcgcca accgcgagca cctcatctac gaccaggatc
      361 tccggctcca ggtgggccgc cacggcaaaa gccagtcgca catacatgcc gctgctgtaa
      421 cgcttgaccg gcgtatcgag gaacttctct acctcggcga aagccacgat ctcgtcgaac
      481 tttttccgga tctccacccg gctcatcccc aaaatcgctc cattgaggaa gacgttctcc
      541 ctgcctgaga gttccggatg aaagccggta ccaacctcca gcagcgaagc cactcttccc
      601 cggatgctaa cctttccccg ggttggctcg gtaatgcggc taaggacctt caacaaggtg
      661 cttttgcctg ccccgtttcg cccgatgata cctactcttt caccgggctg tatttcgaaa
      721 tcgacatcct tcaacgccca aaactcctcc ttgctgccct tttgccgctt gtccgcatgc
      781 ctgccgggaa gcaacctttt tgcgccgcgg gagatcgtat cccgcagggt tgtataccgt
      841 tccggcgcct catgagcgat caggaatctc tttgaaagcg aatccacctt tatgacagaa
      901 ttcgacatga tagatgatta aatgagatcg gcaaaggatt tttccgtttt cctgaaatac
      961 gagatcccca accataaaaa taagagcatg accccgagcg agagaaaaaa gccgggccag
     1021 tacatgggtt cgccgacaat acagtagcga aatccgtcga tgactcctac catgggatta
     1081 agggaaaacc agaatctggc cgtgtccgaa tacttttgcg cgatgacggc gctgctatac
     1141 ccgacaggag taatgtatat cccgaactgt acgacaaacg gaatgatata gcgaaaatcc
     1201 cggaatttga catttaatgc cgttacgaac aaaccgatcc caaacgaaat caggaaggcc
     1261 aacgccgcga ataggggaag gaacagcatc tgtaaaggcg gaacgaagcg gtaaaagagc
     1321 atcatcagca ggagcagtcc gaaggagatt cccaggtcga ccaggctggt aataacagaa
     1381 gccgccggca cgatcatgcg gggaaaatac actttggata tcaggttgac attaccgatc
     1441 atgctgttgc tggcctcggt aagcgcggca gaaaagaatt gccagggcag caggcccgca
     1501 aacacgacga tcgggtaagg aggaccattt ttaacggcca ggttggcgat ccggccgaag
     1561 ataaacgtaa aaatgatcat cgtgagtaag gggcggatca ccccccaggc agcgcctaca
     1621 acggtctgtt tgtaccttac cttaatatca cgccagctga gaatatagaa aagttcccgg
     1681 tatcgccaaa ggtcgcgcca ataatttttt tctactccgc cgggctcaat aattaattct
     1741 tccatccatt ttcgaattta cgtgctgccc atcctggtgc tagccgaacc acctttccag
     1801 ctgcgccctg acccgcctga agatggaacc gttgcccatt cctctttcgt tctcaaaata
     1861 accggagtcg ccgccatagc catacccacc gtaatatcca ccgccgttat aaccaccgta
     1921 gtacccccct tccattttca catcgttcag caataacgat atctttggca atttcttctg
     1981 tgtatataat tcgtcgatcg catttatttg tctgcgaaac gtataggctt gcctgacgat
     2041 atataaggtg cagtccgcaa agcggaccag gttcacggcg tcgctcacca ggccgacggg
     2101 ggccgtatcc atgatgatca cgtcgaatcg tttgtggacc tcggccatca attcgtcaag
     2161 ccggggatcg agcaataatt cggacggatt cggcggaata ggaccgcagg ggatcacgta
     2221 aaggttgtcc acttcatcca ctttaacgac cagttcctcg aattttgcgc tgccgatcaa
     2281 ataattggtg atgcccatct ttcgccttaa ctcgaggccc gacacgatct tgggtttccg
     2341 gatgtcgaat tccatgatga ccgtttttcg gtccgccagc gccattacgg ctccgatatt
     2401 gatacttata aacgatttcc cctccccgct gaaggaggaa gtgaccataa tgacgggttt
     2461 ttcctttttg ccgatgacgt attgcaggtt cgtccggatg atgcggaact gttctgagat
     2521 gaacaggcgg ctattctttg tgaccaccag cgtattttta tagttggagt ggccgatctc
     2581 acccaaaata ggcgccgcgg tattcttttc tacgtctgcc cttccggtta ccttatcccg
     2641 caatacttcg atcagcgcaa tgacggcaca cggaatcagc aacccgagaa gaatatagag
     2701 tgtatatatc ttcttccggt ccgggctgat ttccgtcccg gcgcctgcgg ccggctccac
     2761 caccttggaa ttggaaacgg tggaggctga cgcaatctgc gtctccaatt ttttctgcag
     2821 cagcagggag tacaattcct ccaggatccg ttgctgtcgc tgccggttga gtaactgaat
     2881 ggatttcccg ggcatggacc gaatgccgtt ttgcaattcc tgttcgtggc tctccagttt
     2941 actccccgcg atcttatagg attcctttac atttagcagc gcctggtaaa tctccttccg
     3001 tattccctcc agagaaacct ccatcccgac gacaagggaa ttggtttccg gggtggtccg
     3061 aagcgttgcc tgccgctcca gctgcagccg gttgtattcg gctatcatcg gcaccagagc
     3121 gggctcctgg acacccaggt cggtaggcac caggtcatac gcatgtttct tatccgcaat
     3181 atatttcagc aaccaatcga taatattcaa ccgcacctcc tgttcatccc tgtctttgat
     3241 gacgtctttc aaattgtcca ggtagttttt ggattgtgca tccagatcaa aaacctggtt
     3301 ttcgaccata taattcctca gtcccccctg cgcccccttc agcgtatcat tcagcgtgta
     3361 caattccgta ttgataaagt tcagcgtatt ggcattgatc cggcttttgt cttcgatgat
     3421 cagactgtca tacacactca tcagtgtatt caggacgtcg ttgccaagat tggcattttc
     3481 gccttcgaag gcaagggtca gaatagtgga ctggtcattg gcctgggaca ccgaaagccc
     3541 ccctgccagg ttctccgccg catacttgaa gggttgccag ccggtgctga atttggcatt
     3601 gacgaagctg ttttcgttat aagccggatt ccgcgtcagg cggcaaaggt tattccctac
     3661 cttgaacggc tcgccaaatt cgagtttcgt cttgttctca tccagcagaa acttcccgtt
     3721 ccccagcagg gtgatcatac aattgaatcc cgaggcggaa tccactatcg acaaaggctc
     3781 gaggaaaaag ggcgggtcgg ggtacataaa gctcgtcttt acgttaccct tattataata
     3841 atagatctgg agattcagat cccgcgccac gcgctgcagc accggctttg actttaggat
     3901 ctcgatctcg ttgctgaggt tggaaacccc ctgcgacatg aataactc
//
LOCUS       DATGKE010000733         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1506579, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000733 DATGKE010000000
VERSION     DATGKE010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506579"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VKU83_12450"
     CDS             <1..255
                     /locus_tag="VKU83_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94422.1"
                     /translation="AIDVYPDSHFDVIIVDGRARPSCIKHALPKLKPGGWLLVDNSDR
                     SYYLEPFSFDKTDWKVLTFEGPAPFMKGFSRTTLLQKRHR"
     gene            252..1142
                     /locus_tag="VKU83_12455"
     CDS             252..1142
                     /locus_tag="VKU83_12455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLI94423.1"
                     /translation="MNPAPIALFAYKRPDHLKRVVDSLLRNALAPQSELFIFSDGPRN
                     TEVETAVAELRKYLRTITGFKKVIITERAENFGLAKNIIDGVTSVVDLHGKIIVLEDD
                     LVLSPYYLEFMNEALEKYQDVEEVISIHGYSYPISYAKPVYFLRGADCWGWGTWKRGW
                     DLFNPDTQFLLDEIRRKDLSYAFNMDDSYDYTGMLKAQIEGRVDSWAIRWYASAFLEN
                     KLTLYAYPSLLENIGMDDATHGQDPGLTDTDLTDHRIVLEDIPVEETIAARKLIARHL
                     YRNMPMRAKIKRLLKRGLGS"
     gene            1163..2104
                     /locus_tag="VKU83_12460"
     CDS             1163..2104
                     /locus_tag="VKU83_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94424.1"
                     /translation="MKIIQTILTSAYFSEAPPVLIDIGASGEINAKWRSIAPYSVCLA
                     FDADDREFHITEQANQTYKRLITFNRIVTSAAAKEADFYLTASPFCSSLLSPATDKLK
                     PWVFSELFTVERVAKLPTITIEESLKQVKIDYIDWFKTDTQGTDLRLFTSLPAALQDG
                     ILAAEFEPGIIDAYMGEDKLYSVMQEMHRRAFWLSTMDVKGVQRLRSDQAAGVSPFVA
                     SKMLRKSPGWAEVTYLKQPAALTSRQLLLLYVFALLERQYGFALEITDVAMRQSPDAL
                     FRDCQTAAKEQLTKERRRTPLVFFKRKINKLLSRIHD"
BASE COUNT          543 a          562 c          573 g          491 t
ORIGIN      
        1 gccatcgatg tctatccgga tagccatttc gatgtgatca tcgtcgatgg gagagccaga
       61 ccttcctgta taaaacatgc cttgcccaag ctcaagccag gcggctggct tttggtggac
      121 aattcggaca ggtcctatta cctcgaaccc ttttccttcg ataagaccga ttggaaggta
      181 ttgacattcg agggcccggc tccctttatg aaaggctttt ccagaaccac tcttcttcaa
      241 aagcgtcatc gatgaatcca gcacctatcg ccctattcgc ctataaacgt cccgaccatc
      301 taaaaagagt ggtggattcg ctgcttagga atgcgttggc gccgcagtcc gagcttttta
      361 ttttttccga cgggccgcgt aataccgaag tagagactgc ggtcgccgaa ttaaggaaat
      421 acctacgcac tataaccggg ttcaagaagg tgatcattac cgagcgggca gaaaatttcg
      481 ggcttgcaaa gaacatcata gatggagtga cctcggtcgt tgatcttcat ggtaagatca
      541 tcgtactgga agacgatctg gtcctctcgc cctattatct ggagttcatg aatgaggcgc
      601 tcgagaagta ccaggatgtg gaggaagtga tatccattca cggctacagt tacccgataa
      661 gctatgccaa accggtatat ttcctgagag gcgcggactg ctggggctgg gggacctgga
      721 agcggggttg ggacctgttc aatccggata cccaattcct gctggacgag atccggcgaa
      781 aggacctgtc ctatgcattc aacatggacg actcgtacga ctataccggc atgctgaaag
      841 cacagatcga aggcagggtt gactcctggg cgatacggtg gtatgcctcc gccttcctcg
      901 aaaacaagct cacactatat gcctatcctt ccttattgga aaacattgga atggacgatg
      961 ccacacacgg gcaggacccg gggttgaccg acaccgacct gaccgatcat cgcatcgtat
     1021 tggaggacat accggtggag gagactatcg cggcgagaaa gctcattgcg aggcacctgt
     1081 accggaatat gcccatgagg gccaagataa aaagattatt gaagcggggg ttagggtcct
     1141 aaccgtatcg aatcctttgc aaatgaagat catccaaacc atactgacat cggcctactt
     1201 ttcggaagcg ccgcccgtac tgatagacat cggggcatcg ggggagatca atgccaaatg
     1261 gcgatccatc gcgccctaca gcgtgtgtct tgcctttgat gccgatgacc gggaattcca
     1321 tatcaccgaa caggcgaacc aaacgtataa aaggctgatc acgttcaacc ggatcgtcac
     1381 ctctgcagcc gcgaaagagg cagattttta tcttaccgcg tccccttttt gttccagtct
     1441 gctgtcccct gcgaccgaca aattgaaacc gtgggtattc agcgaactat ttaccgtgga
     1501 acgcgtcgcg aaactgccga cgatcacgat cgaagagtcc ctgaaacagg taaagatcga
     1561 ttatatcgat tggttcaaga ccgatactca gggcaccgat ctgcgtcttt ttacgagcct
     1621 tcctgcggcc cttcaggatg gcatactagc cgcggaattc gagcccggca ttatcgatgc
     1681 ctatatgggc gaagacaaat tgtacagcgt catgcaggaa atgcacagaa gagcgttctg
     1741 gctgtcgacc atggatgtaa agggggtgca gcggctgcga tcggaccagg ccgccggcgt
     1801 ctctccgttc gtcgcgagca aaatgctccg gaaaagtccc gggtgggccg aagtgaccta
     1861 tctgaagcag cccgccgccc tcacttcccg tcagcttctc ctgctgtatg ttttcgccct
     1921 gctcgagcgg caatacgggt ttgccctgga gataaccgat gtggcgatgc gccagtctcc
     1981 ggacgccttg ttcagggatt gccaaacggc ggcaaaagaa cagctgacga aggaaagaag
     2041 aaggacgccg ctggtattct tcaaaagaaa gatcaataaa ttactgagcc gcatacatga
     2101 ttaatttttg cacgctgttc gattcgaatt atttaacgcg ggcgatagcg atgaacgaat
     2161 cgctgtcca
//
LOCUS       DATGKE010000734         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1481721, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000734 DATGKE010000000
VERSION     DATGKE010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481721"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2769)
                     /locus_tag="VKU83_12465"
     CDS             complement(<1..>2769)
                     /locus_tag="VKU83_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLI94425.1"
                     /translation="YVNLLLYVGGVKSIESRNIQGLTLIKLTFYEGTDMAQAASEVTG
                     YITRAQAAFPQGSQPPFILRFDASTLPVGQLVLSSPIRSNNELQDMANIYVRSQFSSV
                     PGLVSPAPFGGNPRTVLININPDLMRAHNLTPDQIVTALRVNNQATPAGNVRIGNINY
                     LTPTNSTIKNIKDFGDIPIYSNGVQNIYLKDVATVEDGGDITLGYALVNGRRSVYLPI
                     TKAAAASTWEVVQNLKKAIPRFQAQLPEDVKLSYVFDQSTYVINSVKNLLSEGAIGAI
                     LTGLMVLLFLGDLRGASIVILTIPICLISGVLFLNLCHQTINIMTLSGLSLAIGILVD
                     ESTVTIENIHQHLAMGKPKSLAIWDACREIAFSKLLILFCILAVFAPAFSMGGIPGSL
                     FLPLALAIGFSMITSYLMAQTFVPVMANWVMKGHKKKGGRRFSSDDEEFRASGLTAAD
                     EASAAEEKHRLLEHGTGDGGRETRFDRFRKRFIRFLDRIMPFRLAIALVYIVLGMAGA
                     FLLINSIGRDVLPKSNQGQFQVRMRAPDGTRVERSEEYMIRTLHILDSMVGHQNIAVT
                     SAMVGMHSAQFSTDPIYLFMAGPQEGVLEAAFKEEYKVNMDDFKEAFRHRIARDMPWM
                     KVSFEPIELTDKILSQGSPTPIEVKILGKNKKLNEEYAGKVIDRLRQIPYLRDVQLDE
                     AIHYPALDIDIDRVRASQLGTTADVISRSLIAATSSSRYTEKNQWIDQKSNLSYSVQV
                     EIPTNEMKSIHDISEIPVVPNAARPVLGDVASITRDSVYGDADDIGSIPVLSVTAGLY
                     KKDLGHATADVQRALASLGKLPRGLVIETRGLTTVLSETLSSLQMGLVVAIVVIFLML
                     AANFQSFKVSLVALCTIPAVLLGSIGILMLTGSTLNLQSYMGMIMSVGVSISNSVLLV
                     TNAEELRR"
BASE COUNT          606 a          788 c          769 g          606 t
ORIGIN      
        1 gtcgtcgcaa ttcctcggca ttggtgacca gcagcacgga attggagatc gaaacaccga
       61 ccgacatgat catccccata tacgattgca ggttcagcgt cgaacccgtg agcatcagga
      121 tgccgatcga acccagcagc accgcgggaa tcgtgcaaag cgccaccagc gataccttga
      181 atgactggaa attggcggcc agcatgagga atatcactac aatagctacg acgagcccca
      241 tctgcaggct gcttagcgtc tcgctcagca ccgttgtcag gccccttgtc tcgatcacca
      301 gcccgcgcgg gagttttccg agagaggcga gcgccctttg cacatctgcc gtcgcatggc
      361 cgagatcttt cttatacagc ccggcagtta cggaaagcac ggggatcgat ccgatatcgt
      421 cggcgtctcc atagaccgag tcgcgcgtga tggaagccac gtccccgagg accggccggg
      481 ccgcattggg aacgaccgga atctcgctga tgtcatggat acttttcatc tcgttcgtcg
      541 ggatctccac ttgcacgctg taagaaaggt tggacttttg gtcgatccat tgattctttt
      601 ctgtatagcg cgaagaagaa gtcgcggcaa tcagtgagcg gctgatgaca tccgcagtgg
      661 taccaagctg ggacgcccgc acccggtcaa tgtcaatatc cagtgccgga tagtggatcg
      721 cttcatcaag ctgtacatcc cgcaaatagg gaatctgcct gagccggtcg atgaccttgc
      781 cggcgtattc ttcgtttaac ttcttgttct ttcccaggat tttgacctcg atcggcgtgg
      841 gcgaaccctg gctgaggatc ttatcggtca gctcgatggg ctcgaaggaa accttcatcc
      901 acggcatgtc ccgggcaatg cggtgccgga acgcctcctt gaaatcgtcc atattcacct
      961 tatactcctc cttgaaagca gcctctaata cgccttcctg cggtccggcc atgaacaggt
     1021 agatggggtc ggtggaaaac tgggcgctgt gcatgccgac catggcggat gttacagcaa
     1081 tattttggtg cccgaccatt gaatcgagga tatgcagggt ccggatcatg tattcctccg
     1141 accgctcgac acgggtgcca tccggcgccc gcatccggac ctggaactgg ccctggttgg
     1201 atttcggcag cacgtcccgc ccgatgctgt tgatcagcag aaaggcgccg gccatcccaa
     1261 gcacgatgta gacgagcgcg atcgccagcc ggaacggcat gatgcgatca aggaagcgga
     1321 taaagcgttt gcgaaagcgg tcaaacctcg tctcacggcc gccgtcgccc gtgccatgtt
     1381 cgagcagcct gtgcttttcc tccgcagcgc ttgcctcgtc ggccgccgtc aggcctgagg
     1441 cccgaaactc ttcgtcatcg gatgaaaaac gtcgcccgcc ctttttctta tgccccttca
     1501 tgacccaatt ggccatcacc gggacaaaag tttgcgccat gaggtaggag gtgatcatcg
     1561 aaaatccgat cgccagcgcc agcggcaaga acaaagatcc gggaatcccg cccatcgaaa
     1621 aggccggggc gaaaacggcg aggatacaga acaggatcaa tagctttgaa aacgcaattt
     1681 cccggcaggc gtcccaaatc gccagcgact tcggctttcc catcgccagg tgctggtgga
     1741 tgttttcgat cgtaacggtg gattcatcga cgaggatgcc gatcgccagc gacaggccgc
     1801 tcagcgtcat gatattgatg gtctgatgac acagattcag aaaaagcacg ccggagatca
     1861 ggcatatcgg gatcgtgagg atcacgatcg acgcgccgcg caggtcgccg aggaacagta
     1921 ggaccatcaa ccctgtaaga atggctccga tggcgccctc gctcaacagg ttcttcactg
     1981 aattgatgac gtaggtcgac tgatcgaata cgtaggataa tttgacatcc tccggcagtt
     2041 gcgcctgaaa gcgcgggatc gctttcttta ggttttgtac tacttcccag gttgaagccg
     2101 cagcggcttt ggtaatcggc aggtaaaccg atcgtcttcc gttgacgagt gcatacccga
     2161 gtgtgatatc gccaccatcc tccaccgttg ccacgtcctt cagatagatg ttttgcaccc
     2221 cgttgctata aatcggaata tccccgaaat ccttgatgtt tttgatggtc gaattcgtcg
     2281 gcgtcaggta attaatgttc ccgatccgga cgttccccgc cggggtcgcc tggttgttca
     2341 ctctcagtgc agtaacgatt tgatcgggcg taagattatg tgcccgcatc aggtcaggat
     2401 tgatattgat cagcaccgtc cgcggattgc cgccgaacgg cgccggcgat accagacctg
     2461 gaacgcttga gaactgcgat cggacataga tattcgccat atcctgcagt tcattgttgc
     2521 tccggattgg gctgctcagc accagttgac ccaccggcag ggtcgaggcg tcgaaacgaa
     2581 ggataaaagg tggctgcgac ccctgcggga aggccgcctg cgcgcgggtg atatagcctg
     2641 ttacctcaga agctgcctgg gccatgtcgg tcccctcata aaaagtgagc ttaatgagcg
     2701 tcaatccctg gatattccgg ctctcgatgc tcttgacccc gccgacgtag agtagcaggt
     2761 tgacgtatt
//
LOCUS       DATGKE010000735         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_264827, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000735 DATGKE010000000
VERSION     DATGKE010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264827"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2227
                     /locus_tag="VKU83_12470"
     CDS             <1..>2227
                     /locus_tag="VKU83_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HLI94426.1"
                     /translation="LHYEMSNEIGKAQREWEAIGSVTDAWDLAGPFDNVSGSGYYKDY
                     GPLDHPEADASFVSFNNAAVRWFVPTVMSKEGYTNPYPHFRRNTAIIYAQSFVYSPDD
                     RQVLINVGVCGSVKVWLNDQPVLACPKELVTELDYYRQRITLRKGYNRLLIQLGYTNS
                     STPNFIVRFTNDRYFPVTGLTYTAAYQPYPKAAAGAAMAEAPAPIRHFAEDFFEKKVA
                     AEPDNYINYLLLSDVYLRSQKTVEARRTVEKALQHAPDNSLLRLQLMEILMKEENRTL
                     LLEEVERMKDKDPDCLIAIRLNIRKLAGEEKYDEAFKELDRCVQLYGEDDDSWIERIT
                     LYSKQNKMDDLVKAIQTAYEKYPDNSEVVDWMYSLKVNGQKDAKGGIKVLENYLKDNY
                     SYSMIEKLTAEYTKQGEKEKALKLLQQPKEAFPYDPDLYTDISSFYYDQEQYSKAVDF
                     GRQALRLAPYVATYWENLGLELQQEHQDSEAVKAYLQALYYDANKYSAREHLRDLRKK
                     PSVWKAFPDDDVYQAIRNVADRQYDYDYFYVLDEKFAVVYPEGASEEYTTTVVKILTQ
                     KGIDTWKEDDLGYNSNTQELKVEKAEVVKKNGTVVPAEQNDNQVVFTGLEVGDAIVLK
                     YKVQNYAEGRVTRHYWDRFGFNATVPEDTVRYCLLVSDKIKFDYQVDKADLKAKKTPY
                     DDFTLYSWEMVKPAICKSEPFMPPLMDVGPTLHVSTLTSWNEIASWYSDLATSKTDDD
                     FE"
BASE COUNT          575 a          589 c          640 g          423 t
ORIGIN      
        1 ctgcattatg agatgtcaaa tgagatcgga aaggcgcaaa gggaatggga ggcgatcggc
       61 agtgtgaccg atgcctggga cctggcgggt cccttcgaca acgtatcggg gagcggctac
      121 tacaaagatt acgggccgct agaccacccc gaggcggatg catcgttcgt ctccttcaac
      181 aatgccgcgg tcagatggtt tgtgcctacc gtgatgagca aggaaggtta caccaatccc
      241 tatccgcatt tccggcgcaa caccgcgatc atctatgcac aaagctttgt ttattcgccg
      301 gacgaccggc aggtactgat caatgtcggg gtctgcgggt cggtcaaggt ttggctcaac
      361 gaccagccgg tgctggcttg cccgaaagag cttgtcacgg aactcgacta ttatcgccag
      421 cgcattacac tcaggaaagg ctataaccgc ctgctgatcc agctcgggta taccaactcg
      481 agcactccga attttatcgt ccggtttacc aacgacagat atttcccggt caccggcctg
      541 acctacacgg cggcctacca gccttatcca aaggcggctg ccggagcggc catggcggaa
      601 gccccggcgc caatccggca tttcgccgaa gacttctttg aaaagaaggt cgccgccgaa
      661 ccggataact atatcaatta cctcctgctt tccgatgtct acctgcgcag tcaaaagacg
      721 gtggaagccc gccgcaccgt tgagaaggcg ttgcaacacg cgcctgacaa ttccttgctg
      781 cggctccagc tgatggaaat cctgatgaag gaagaaaacc ggacgctgct cctcgaggaa
      841 gtggaacgca tgaaagataa agaccctgac tgcctgatag cgatccggct caatatccgg
      901 aaactggcgg gcgaagaaaa atacgacgag gccttcaagg agctcgatcg ttgcgtacaa
      961 ctctacggtg aggacgacga cagctggatc gagcggatca cgctgtattc gaaacaaaac
     1021 aagatggatg atctcgtcaa ggccatccag acggcatatg aaaaatatcc ggataactcc
     1081 gaggtggtgg attggatgta cagcctgaag gtgaacggcc agaaggatgc gaaaggcggg
     1141 ataaaagtgc tggaaaacta tctcaaggat aactacagct atagcatgat cgagaagctt
     1201 accgccgaat ataccaagca gggtgaaaag gagaaggcgc tcaaactgct gcagcagccg
     1261 aaggaagcgt tcccctacga tcccgatctg tataccgaca tatccagctt ttattacgat
     1321 caggaacaat acagcaaagc tgtggacttc ggcaggcagg ccctccggct ggctccctac
     1381 gtggcaacgt attgggagaa cctcgggctc gagctgcagc aggagcacca ggactcggag
     1441 gcagtaaagg cctacctgca ggccctctat tacgatgcca acaaatattc ggcgcgggag
     1501 caccttcgcg acctgcggaa gaagccgtcc gtttggaagg cctttccgga cgacgatgtg
     1561 tatcaggcca tccggaacgt cgccgaccgc caatatgact acgactattt ctatgtgctc
     1621 gatgaaaaat ttgcggtcgt ctacccggaa ggcgcctccg aggaatatac taccaccgtg
     1681 gtgaaaatcc tgacgcagaa gggaatcgat acctggaaag aggatgacct cggctataac
     1741 tcgaacacgc aggagctcaa ggtcgaaaag gcggaggtgg tgaagaagaa cggcaccgtc
     1801 gtgccggcgg agcaaaatga caaccaggtg gtgttcacgg gcctggaagt gggggacgcg
     1861 atcgtcttga aatataaggt ccagaactat gcggagggcc gcgtcacgcg gcattattgg
     1921 gaccgtttcg gcttcaacgc cacggtgccg gaggacaccg tcaggtattg tttgctggtg
     1981 tcggacaaga tcaagttcga ctaccaggtg gacaaagccg acctgaaggc gaaaaagacg
     2041 ccctatgacg attttacgct ttattcctgg gagatggtca aaccggcgat ctgtaagagc
     2101 gagccgttca tgccgccgct gatggacgtg ggcccgacac tgcatgtctc cacgctgacc
     2161 tcgtggaatg aaatcgccag ctggtacagc gatctggcga cgagcaagac cgacgacgat
     2221 ttcgagg
//
LOCUS       DATGKE010000736         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1094456, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000736 DATGKE010000000
VERSION     DATGKE010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094456"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /gene="pckA"
                     /locus_tag="VKU83_12475"
     CDS             <1..1386
                     /gene="pckA"
                     /locus_tag="VKU83_12475"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HLI94427.1"
                     /translation="IHWNDFNQPIDPAHFHRIFTAVTSWLNQAPELFVRDCYVCADHR
                     YRQKIRSINETPSGNLFAYNMFLRPAEEELEDFQPDWQILAAPGLALNPAECGTRSAN
                     AVAISFRHRLIVLAGTAYTGEIKKGVFSILNFLLPTERNVLSMHCSANKGPKGDTAIF
                     FGLSGTGKTTLSADPARRLIGDDEHGWTDDNIFNFEGGCYAKCVNLREDKEPDIYRAI
                     RPGALVENVKFFPGTDRINFDDCSITENTRVSYPLHYISNACEPSIGAPPANIFFLTC
                     DAYGVLPPISRLSPGQAMYQFISGYTAKVAGTEAGVTEPRPTFSACFGAPFLPLHPAR
                     YAEMLGEKIKQQGAKVWLVNTGWSGGPYGQGTRIPLAFTRKMITAALDGSLDKMEFHP
                     HPVFGMAVPTGCPGVPTKLLHARGTWPSAAAYDEMAANLADWFTTNFEKYATEIRSPE
                     ILAAAPGRRPH"
     gene            complement(1594..2229)
                     /locus_tag="VKU83_12480"
     CDS             complement(1594..2229)
                     /locus_tag="VKU83_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLI94428.1"
                     /translation="MLKNKKAKHIAVAGNIGAGKTTLTEMLSKHYRWIPQFEDVDHNP
                     YLNDFYEDMPRWSFNLQIFFLNTRLNQLLEIHAGTETVIQDRTIYEDAHIFAPNLHEM
                     GLMSKRDYNNYFQFFQTLKRMVQPPDLLIYLQASVPTLVAQIQKRGREYEENIRLDYL
                     KRLNDFYNKWIEGYKEGPLLIVDIDKNKFPENEEHLGEIIQRIDAQLYGLF"
     gene            complement(2274..3338)
                     /gene="trpS"
                     /locus_tag="VKU83_12485"
     CDS             complement(2274..3338)
                     /gene="trpS"
                     /locus_tag="VKU83_12485"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLI94429.1"
                     /translation="MEKKKEIVMSGIRPTGFLHLGNYFGAVRNYVRMQENYQCFFMVA
                     DWHALTTMLETKGLQGHVRRVMAENIASGLDPEKVALYCQSHVPETAELYLLLNMLAY
                     KGELEKTPTFKEKVRLQPQNVNAGLLTYPVLMAADILLHRARLVPVGKDQEQHLEIAR
                     NFADRFNSRYGDVFPDPYAFNFGEQLVKVPSLDGAGKMSKSENQNATIYLADSDDIIR
                     SKIRAAKTATGGGARSENAEPASGSEAGMPATGGVRAGMTPPVANLFLLLELVASSDV
                     VSKFEADYTAGTIRYSDMKGELAEGMVKFIAPIREKAIAIQRDEPYLNRVMEQGADKA
                     RASADETLKLVRQAIGLKYY"
     gene            3440..3733
                     /gene="gatC"
                     /locus_tag="VKU83_12490"
     CDS             3440..3733
                     /gene="gatC"
                     /locus_tag="VKU83_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLI94430.1"
                     /translation="MEVNDELIANLANLARLEFDATEKAQIRVDLQRMIEFVERLGEL
                     DTTGVEPLLHLSCETDVLRDDVPGGSANRDEVLSNAPSTDGVYFKVPKVIRKS"
     gene            3730..>4461
                     /locus_tag="VKU83_12495"
     CDS             3730..>4461
                     /locus_tag="VKU83_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLI94431.1"
                     /translation="MIANTISAGVDSRGKPIPIIRLVDIHKSYFMGKQALPVLKGVSL
                     TIEKNEYVALMGPSGSGKSTLMNILGCLDTPTDGSYILNGQDVSKMPDDDLADVRNAE
                     IGFVFQQFNLLPRLTAAENVALPLVYAGVPRRQRLERAMAVLEKVNLTDRSHHKPNEL
                     SGGQCQRVAIARALINNPALILADEPTGNLDSKTSVEIMDIFTSIQAAGNTVVLVTHE
                     EDIANYARRIIRLRDGMIESDRLRRP"
BASE COUNT          976 a         1370 c         1117 g          998 t
ORIGIN      
        1 atacactgga atgacttcaa ccaacctatc gatccggccc acttccatcg cattttcacg
       61 gccgtgacaa gctggttgaa ccaggcgccg gagctcttcg tgcgtgattg ctacgtctgc
      121 gccgatcacc ggtaccggca gaagatccgc tccattaatg aaacgcccag cggaaatctc
      181 ttcgcatata atatgttcct ccgaccggcc gaggaggagc tggaagattt ccagccggac
      241 tggcagatcc tggccgcgcc cggcctggcc ctcaacccgg cggaatgcgg cacgcgaagc
      301 gccaacgcgg tggccatctc gttccgccat aggctcatcg tactggccgg caccgcctat
      361 acgggcgaga tcaagaaagg cgttttcagt atcctcaact tcctgctgcc cactgaaagg
      421 aacgtattga gcatgcattg ctccgccaat aaagggccca agggcgatac cgccatcttc
      481 ttcggtctca gcggcacggg aaagacgacg ctcagcgccg acccggcgcg ccgcctcatc
      541 ggcgacgatg aacatgggtg gacggacgat aatattttca attttgaagg cggctgctac
      601 gctaaatgcg tcaatcttcg cgaagacaag gaaccggata tttaccgggc catccggccg
      661 ggcgcactgg ttgaaaacgt caagtttttc cccggcaccg accgcatcaa cttcgacgat
      721 tgctccatca ccgaaaacac ccgggtctcc tatccactac attatatcag caacgcctgc
      781 gagccctcaa tcggcgcgcc tcccgccaat atcttcttcc ttacctgtga cgcgtatggc
      841 gtcctgcctc cgatctcccg gctcagcccg ggccaggcga tgtatcaatt tatttccggc
      901 tatacggcaa aagtggctgg cacggaagcc ggcgtcaccg aaccccgtcc aacgttcagc
      961 gcctgctttg gcgcgccgtt tcttccgctc catcctgccc ggtatgcaga aatgctcggc
     1021 gagaagataa agcagcaggg cgccaaggtc tggctcgtga ataccggatg gtcgggcggg
     1081 ccctacggcc agggcacccg catcccgcta gcttttacgc ggaaaatgat caccgcggcg
     1141 ctggacggct cactggataa aatggaattc catccccacc ccgtctttgg aatggctgtc
     1201 ccgacaggct gccccggcgt ccctacgaag ctgcttcacg cacgcggtac ctggccgtca
     1261 gccgccgcct atgatgagat ggctgccaac ctcgccgatt ggttcacaac aaattttgag
     1321 aaatacgcca ccgaaatccg gtcgcccgaa atccttgcgg ccgcgcctgg ccgcaggccc
     1381 cactagctat ccgcaccggc gggttaaaaa aaagttatgg aaaaaatgcg gattataaaa
     1441 taaaatccag aacttggcgc acacaattca cgggcctgcc cggggattgt acacagtctg
     1501 cgattgtttg ccaaatctaa ctccatcatt gcactggtgg ggttttttat tttacacgcc
     1561 gaatcttaac gaagggtggt tgtcgccccc caactaaaat aatccatata actgcgcgtc
     1621 gatccgctgg atgatctcgc caagatgctc ctcgttttcc ggaaatttat tcttatcgat
     1681 atcgacgatg agcaagggcc cttccttgta gccctcgatc cacttattat aaaagtcatt
     1741 tagccttttc agataatcca accggatgtt ttcctcgtat tccctccccc gcttctggat
     1801 ttgcgcaacc agggtcggca cacttgcctg caagtagatc agcaggtccg gcggttgcac
     1861 catccgcttc aatgtttgga aaaactgaaa ataattattg tagtcccgct tactcatcaa
     1921 gcccatctca tgcaggttgg gcgcgaagat gtgagcgtct tcgtagatcg tccggtcctg
     1981 gatgaccgtc tcggtgccgg catgaatctc caaaagctga ttgagccggg tattcaaaaa
     2041 aaagatctgc aggttaaagc tccacctggg catatcctcg tagaaatcat tcaaataagg
     2101 gttgtggtcc acatcttcga actgcgggat ccaacgatag tgcttactca acatctccgt
     2161 cagggtggtc ttccccgccc cgatgttccc ggctacggca atatgtttgg ctttcttgtt
     2221 ctttaacatg atattctcct gttgaaattc gacgggctcc gcccggttca gtattagtag
     2281 tacttcaacc cgatcgcctg cctcaccagc ttcaatgtct cgtctgcact cgcgcgcgcc
     2341 ttgtccgcgc cttgttccat gacccggttc aaatagggtt cgtcccgctg gatcgcgatg
     2401 gccttctccc ggatcggcgc gataaatttt accatccctt cggccagctc tcctttcata
     2461 tcactgtaac ggattgtacc tgcggtataa tccgcttcga atttactgac tacgtcggaa
     2521 ctggcaacca gctcgagcag taaaaacaaa ttggcgaccg gcggggtcat gccagccctg
     2581 acgccacccg ttgcgggcat cccggcttcg ctccccgatg caggctcggc gttttccgac
     2641 ctggcccctc cccccgtcgc cgtcttggca gcccggatct tgctccggat aatgtcatca
     2701 gaatcagcta ggtaaatggt cgcgttctga ttttcacttt tgctcatctt gccagctccg
     2761 tccaggctcg gaaccttgac cagctgctcc ccgaaattga aagcgtaggg atcggggaat
     2821 acgtccccat accggctgtt gaaacgatcc gcaaagtttc gcgcaatctc caggtgctgc
     2881 tcctgatcct ttccgaccgg tacgaggcgc gcccggtgca ggagaatgtc cgccgccatc
     2941 aggacgggat aggtcaacaa gccggcgttc acgttttgcg gctgcaatcg caccttctcc
     3001 ttaaaagtgg gtgtcttctc cagctcccct ttatacgcca gcatatttaa aaggagatac
     3061 agttctgcgg tctcgggcac gtgactctgg caatacaacg ccactttttc cgggtcgagc
     3121 cccgaggcga tgttttcggc cattacccgc cggacatgtc cctgtaatcc ctttgtttcc
     3181 agcatggtcg tcagggcatg ccaatccgcc accataaaaa aacactggta gttctcctgc
     3241 atccgcacgt agttccgaac ggcgccaaaa taattgccaa ggtgcaggaa tccggtgggc
     3301 cggataccgc tcataacgat ttctttcttt ttttccattt ttgataccga tctttcagtg
     3361 tccgcagcgc ccgccggagt tgccaaccgc aactttgaaa gaagcgcgaa gatagctatt
     3421 tttgtcccaa agcacccgca tggaagtcaa tgacgaactg attgccaatc tcgccaatct
     3481 tgcccgcctg gaattcgacg ccaccgagaa agcgcagatc cgggtggacc tgcagcggat
     3541 gatcgaattc gtggaacgcc tcggcgagtt ggatacaacg ggtgtcgaac ccttgcttca
     3601 cctgagctgc gaaaccgacg tcctgcgcga tgacgttccg ggcggctccg caaaccgcga
     3661 tgaagtgctt tcaaacgccc cgtcgacgga cggcgtctat ttcaaagtcc cgaaagtgat
     3721 ccgaaaatca tgattgctaa caccatatct gcaggagtcg attcccgcgg gaaacctatt
     3781 cccattatcc gcctcgtcga catccataag agctatttca tgggcaagca agccctacct
     3841 gtcctcaagg gcgtttcact caccatcgag aaaaatgaat atgtcgcgct gatggggccg
     3901 tcaggttcag gcaaaagcac cctcatgaat attctgggct gcctcgatac gcccaccgat
     3961 ggcagctaca tcctcaatgg ccaggacgtc agcaaaatgc cggacgatga cctcgccgac
     4021 gtccgcaacg cggagatcgg atttgtcttc cagcagttca acctgctgcc caggctgaca
     4081 gcggcggaga atgtcgcgct tccgctggtg tatgcgggcg tcccgcgccg acagcggctg
     4141 gaacgggcca tggccgtgct ggaaaaggtc aatctcaccg atcgcagcca tcataagcca
     4201 aacgaacttt ccggtggtca atgccagcgc gtcgcgatcg cccgcgccct gatcaataac
     4261 cccgccctga tcctcgccga cgaaccgacg ggcaaccttg actcgaagac ctccgtcgag
     4321 atcatggaca ttttcacctc tatccaggct gcagggaata ccgtcgtcct ggtcacccat
     4381 gaagaagaca ttgcgaatta tgcccggcgg atcatccggc tccgcgatgg catgatcgaa
     4441 agcgaccggc ttcgccggcc g
//
LOCUS       DATGKE010000737         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1094923, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000737 DATGKE010000000
VERSION     DATGKE010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094923"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VKU83_12500"
     CDS             complement(<1..276)
                     /locus_tag="VKU83_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94432.1"
                     /translation="MATHKHPPASLIFIFGGSGDLNYRKLSPALYNLFIDDWMPEKFG
                     IVGIGRTPYNDEDYRKRLLNGIQQFSRRKGEANGHWDDFSKHVSYLQM"
     gene            complement(300..1721)
                     /gene="gndA"
                     /locus_tag="VKU83_12505"
     CDS             complement(300..1721)
                     /gene="gndA"
                     /locus_tag="VKU83_12505"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HLI94433.1"
                     /translation="MANINFKFGMIGLGVMGRNLLLNMADHGFAVVGFDKDGTKTATL
                     ESSATPGTTVKGVNTLADMVRKLERPRRMMMLVPAGKPVDDVIHDLLPLLEKGDIVID
                     GGNSHYTDTLRRVAFMEEKGFHFMGVGISGGEQGARTGPSIMPGGDPDAWPHVKALLE
                     AVAAKVDGEPCVAHLGKGAAGHYVKMVHNGIEYAIMQLISESYDLLHRGLGLDNDALY
                     KIYKNWNEGELQSFLIEITRDIFQMKDDKTGNYLVDMILDKAGAKGTGKWTSQDAMDL
                     GVPIPVIDMAVCMRELSAYKEERTQAAGLYKLPPTDIAVPQEVFIQQVQDALLFAIIV
                     SYAQGLAQLHVASKELGMDIPMQDVVKVWRGGCIIRSSLLPVFFKAYQSDPALPNLLL
                     EPEIVQLLQTKKLNIRNVIIHAVHLGYPVAGLMSALSYFEAYRSERMPTNLIQAQRDY
                     FGAHTYQRIDEPGIFHTEWEKRP"
     gene            complement(1893..2303)
                     /locus_tag="VKU83_12510"
     CDS             complement(1893..2303)
                     /locus_tag="VKU83_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLI94434.1"
                     /translation="MLNKKKFVVLAALAGLTAIGITAMRPADDPPGPKNLKVLPKHIS
                     HDDLMKTMDSWRTALGVKCNFCHAASADSTAHRLDFASDAKPEKNIARGMYKMAIKIN
                     KKFFKKIKDDQGQPMQVVTCKTCHHGSPHPNEAK"
BASE COUNT          507 a          667 c          733 g          626 t
ORIGIN      
        1 catctggagg tacgagacgt gtttggaaaa atcgtcccaa tggccgttgg cctccccttt
       61 ccgccggctg aattgctgga tgccattcag aagccgcttg cggtagtctt cgtcgttata
      121 gggcgtgcgg ccgattccga cgatgccgaa tttctccggc atccaatcgt caatgaaaag
      181 gttatagagt gccggcgaaa gcttgcggta gttgagatcg ccgctgccgc cgaagatgaa
      241 tatgagggaa gccggtggat gtttgtgagt tgccataaaa ggtctgttac gtcattagct
      301 tacggcctct tttcccactc cgtgtgaaaa atgccgggtt cgtcgatgcg ttggtatgta
      361 tgtgcgccga aatagtcgcg ttgtgcctgg atgaggttcg taggcatgcg ttcgctgcgg
      421 taggcttcaa aatagctcag cgcagacatc aggccggcaa cgggataacc gaggtggaca
      481 gcgtgaatga tcacgtttcg aatattgagt ttctttgtct gcagcagctg gacgatctcc
      541 ggctccagga gcaggttcgg tagcgcgggg tcactctggt acgccttgaa gaaaacaggg
      601 aggagcgaag accggatgat gcagccgccg cgccacacct tgacgacatc ctgcatggga
      661 atatccatgc ccagctcttt tgacgcgaca tgcagctgcg cgagtccctg ggcgtagctc
      721 acaatgatgg cgaatagcag cgcgtcctgg acctgctgga tgaaaacttc ctgcgggacg
      781 gcgatatccg tgggtggcag tttatacagg ccggcggcct gggtccgctc ttccttatac
      841 gcggaaagct cgcgcatgca taccgccata tcgatcaccg ggatgggtac cccgagatcc
      901 atggcgtcct gggacgtcca tttccccgtg ccctttgccc cggctttgtc aaggatcatg
      961 tcgaccaggt agttgccggt cttatcgtcc ttcatttgga agatgtcgcg ggtaatctct
     1021 atcaggaagg actggagctc accctcattc cagttcttat agatcttgta cagcgcgtcg
     1081 ttgtccagtc cgagcccgcg atgcagcagg tcgtaggatt cgctgatgag ctgcatgatc
     1141 gcatattcga taccgttgtg aaccattttg acgtaatggc cggccgcgcc tttgccgagg
     1201 tgggcaacgc acggctcccc atcgaccttc gcggcaacgg cttccaggag ggctttcaca
     1261 tggggccatg cgtcggggtc gccaccaggc attatactgg gcccggtgcg ggcgccctgc
     1321 tcgccgccgg agatgcctac gcccatgaaa tggaaccctt tttcttccat aaaggccacg
     1381 cggcggagcg tatcggtata gtgcgagttg ccgccgtcta tgacgatatc gcctttttcg
     1441 agcagcggga gaaggtcatg tatgacgtcg tcgacgggtt tcccggccgg aaccagcatc
     1501 atcatccggc gcggccgttc gagcttgcgc accatgtccg ccagggtgtt gacccctttg
     1561 acggttgttc cgggcgtcgc cgaggattca agcgtagcgg tttttgttcc gtccttgtca
     1621 aagcctacga ctgcaaaacc atgatcggcc atattgagca ggaggttccg tcccatcaca
     1681 ccaagtccga tcattccgaa tttaaagttg atgtttgcca tctctgggat tgtattaaaa
     1741 cattattcaa tagcttgatt caatggataa gcagggccaa atttaggcaa tttaatggtt
     1801 gcggcctgcg gtggcaggcg tcagggggca taaaataagg cggccgcgca ctttgtgcgc
     1861 ggccgcctca ctatgtcgtg ttgagatctg acctatttcg cttcgttggg atggggactc
     1921 ccgtggtgac aggtcttgca ggtgacgacc tgcatcggct ggccctgatc atccttgatt
     1981 ttcttaaaga atttcttgtt gatcttgatg gccatcttgt acatgccccg ggcgatattt
     2041 ttttccggtt tggcgtcact ggcaaaatcg aggcgatgcg cagtgctgtc cgcagacgca
     2101 gcatgacaga agttgcattt gacgccgaga gcggtccgcc atgagtccat ggtcttcatc
     2161 aggtcgtcgt ggctgatatg tttcggcagg accttgaggt tcttcggtcc gggcggatcg
     2221 tcggccggcc gcatagccgt tattccaatt gctgtcaggc cggcaagggc ggcaaggact
     2281 acgaattttt tcttattcaa catagcgctg attttggccc tccaagatag ggaattaacc
     2341 gatacggccc gccagcattt tgacaatatt ttgacaatag cagcgcacgc gccgctttgt
     2401 acctttgccg gcatgagcga accgcgagta aacgacggat tgagcagggc acatattatt
     2461 atcatggcca tatgtactgg gctcgtcgtc gccaacatct actattgcca gccgctgatc
     2521 gtacttattg cga
//
LOCUS       DATGKE010000738         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1253908, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000738 DATGKE010000000
VERSION     DATGKE010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253908"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VKU83_12515"
     CDS             <1..400
                     /locus_tag="VKU83_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLI94435.1"
                     /translation="SAHFYDTADGGILDRFGDIQLEANIEYRFPLGTVLDTKLQSAIY
                     VDAGNIWDRHPIDNSAAEVGSDFQLSSFYKAIAVDAGTGLRLVFPWLLIRLDYAYKIR
                     DPEDIYFPDTWFHGFNLLKGQFQLGIGYPF"
     gene            complement(414..1124)
                     /gene="truB"
                     /locus_tag="VKU83_12520"
     CDS             complement(414..1124)
                     /gene="truB"
                     /locus_tag="VKU83_12520"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLI94436.1"
                     /translation="MDNEKHVFEQGQVLLIDKPRDWTSFDVVRKLRRLVRTKKVGHAG
                     TLDPLATGLLILCTGKFTKRINEYMAREKEYTGVVTLGAVTPTYDLESDPVACGDYAG
                     VRRDRLEEVIAARFVGNIMQVPPIHSAIKVKGQRVYELARSGRDVQLEPRPVRISEFE
                     VTAFHLPDIAFRVVCSTGTYIRSLANDLGAALGCGGYLSALRRTRIGEFRVEDAMTMD
                     QAEEYIRGVFGEGSVGPD"
     gene            complement(1139..2485)
                     /locus_tag="VKU83_12525"
     CDS             complement(1139..2485)
                     /locus_tag="VKU83_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLI94437.1"
                     /translation="MSFLSRFYKSLFISRRWYLLAVMVIVLFVVSYGIPVLFTVAQLM
                     LLFLLVVTVLDYLVLFATRQPVEVRRIMAERFSNGDDNPVRLEIRNRYPFPVRLRVID
                     EVPEQFQLRDFVLETGMRAGGDGSLDYNLRPVQRGEYLFYDINVYIRSPFGLIVRRKV
                     IEAGTTIRVLPSFRTLKQFDLLAMSQNLAESGNKRMRKLGQSLEFEQIKEYVAGDDLR
                     SVNWKATARKGGQLMANTFTDERSQQVYCLIDKGRVMKMPFDGMSLLDFAINASLVLL
                     RVALVRQDKAGLLTFADHVGTFLPAGRKTTQMSHILEILYNQQTKYQESDYEKLHAMV
                     RTRITQRSLIVLFTNFESLSGLQRQLPYIRSIARQHLVLVVFFENTGLGQLREMEAAT
                     IEEVYTKTIAEKFAYEKRLIVKELQQHGIATILTAPENLTVETVNKYLEIKSRLAI"
     gene            complement(2496..3473)
                     /locus_tag="VKU83_12530"
     CDS             complement(2496..3473)
                     /locus_tag="VKU83_12530"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLI94438.1"
                     /translation="MEENLFEQRTDLEDLQRAVQELRAQIGKVIVGQEQMIELLLAGI
                     LADGHILIEGVPGVAKTLTAKLLSKVISVGFSRIQFTPDLMPSDVIGTSVFNPRESAF
                     QFKPGPIFSNIVLIDEINRAPAKTQSALFEVMEERQVTVDGQTYPLHPPFMVVATQNP
                     IEQEGTYHLPEAQLDRFLFKIDVLYPSSEEEFHIVANHHRLRGDVRIDTVQSVLTAER
                     IGDLRTKVRGVHVEEKLIQFITAIVGATRNHKSVYLGASPRASIGILNGSKAIAAMRG
                     RDFITPEDIVYTAPAVLRHRLVLTPEKEMEGASADEVIAGIIRSIEVPR"
BASE COUNT          717 a         1061 c          926 g          802 t
ORIGIN      
        1 ctcggcccat ttctatgaca cggcggacgg cggcatcctc gaccgcttcg gcgacatcca
       61 gctggaagca aatatcgagt accgcttccc gctgggtacg gttctcgaca ccaaactgca
      121 gagtgcaatc tatgtcgacg caggtaatat ctgggaccgg caccctatcg ataattccgc
      181 agctgaagtc ggcagcgact tccagcttag cagcttttac aaggccatcg cggtggacgc
      241 cggcaccggg ctccgcctgg tctttccctg gctcctcatc cggctggatt atgcgtataa
      301 gatcagagat cccgaggaca tttatttccc ggacacctgg tttcacggtt tcaatctcct
      361 caaggggcag ttccagctgg gcatcggcta tcctttctga accccgtccg ccgtcaatcc
      421 gggcctactg acccttcgcc gaaaacgccg cgaatatatt cctccgcctg gtccatcgtc
      481 atcgcatcct ccacccggaa ttccccgatc cgcgtcctgc gtaaagcgct caggtagccg
      541 ccgcaaccta aagccgctcc caggtcattt gccaggctcc ggatatacgt acctgttgaa
      601 cagacgaccc tgaaggctat atccggcaag tggaacgccg tgacctcaaa ttcagaaatt
      661 cttaccggcc ggggttcgag ttgcacatcc cggccgctgc gcgcgagctc gtacacccgc
      721 tgtcctttta ccttgatggc cgaatggata ggcggcacct gcatgatgtt tccgacgaaa
      781 cgcgccgcga tcacttcctc gaggcgatcc cggcggaccc cggcgtagtc cccgcaagct
      841 accgggtcgc tttcgaggtc ataagtcggc gtgacggcgc ccagcgtgac cacaccggta
      901 tattccttct cccgggccat gtactcattg atccgtttgg tgaatttccc ggtgcagagg
      961 attaacagtc cggtggccag tggatcgagc gttcccgcat ggcccacttt ctttgtcctc
     1021 accaggcgcc tcaatttgcg gaccacgtcg aaggaggtcc aatcgcgcgg cttatcgatc
     1081 agcaatacct gaccttgctc gaagacatgc ttctcattgt ccattggcaa atgtaaccct
     1141 agattgcaag cctcgatttt atctcgagat atttattcac ggtctcgacc gtcaggttct
     1201 ccggggccgt gaggatggtc gctatcccat gctgctgcag ctctttcact atcagtcttt
     1261 tttcgtaggc aaatttttcg gcgatcgtct ttgtgtacac ctcctcgatc gttgcggcct
     1321 ccatctcccg aagctggccg agaccggtgt tctcaaaaaa gacgactaag acgaggtgct
     1381 gccgtgcaat ggaccggatg tacggaagct gtcgttgtaa ccccgacaag gattcaaagt
     1441 ttgtgaataa cacgatcagg ctgcgctgcg tgattcgggt ccgcaccatc gcatgaagct
     1501 tttcgtaatc gctctcctgg tatttcgtct gctgattgta gagtatctcc aggatatggc
     1561 tcatctgagt cgtcttccgg cccgcgggca ggaaggtacc gacatggtcg gcaaaagtga
     1621 gcaagccggc cttgtcctgg cgcacgagcg ccacccgcaa caggaccagc gaggcattga
     1681 tcgcaaagtc gagcaggctc atgccatcga atggcatctt cattacccgc cctttgtcga
     1741 tcaggcaata gacctgctgg ctcctttcgt cggtgaaagt attggccatc agctggccgc
     1801 ccttcctggc agtcgccttc cagttgacgc tgcgcaggtc atcgcccgcg acgtattctt
     1861 tgatctgctc gaattcaagg ctttgcccga gctttcgcat ccgtttgttt ccgctttcag
     1921 ccagattttg cgacattgcg aggaggtcaa attgcttcag cgtccggaat gatggcagca
     1981 cccggatcgt ggttccggcc tcaatgacct tccgccggac gattaacccg aaggggctcc
     2041 tgatatagac attgatatcg taaaaaaggt attctccgcg ctggaccggt cgcaggttgt
     2101 agtccaatga cccgtccccg ccggcccgca tcccggtctc cagcacgaag tcccgaagct
     2161 ggaactgttc cgggacctcg tcgatgacgc gaagccggac cggaaaaggg taccggttcc
     2221 tgatctcgag ccggacgggg ttgtcatcgc cattactgaa cctctcggcc attatccgcc
     2281 ggacctcgac aggctggcgt gtggcaaaca gtaccaaata atccagtacc gtcacgacca
     2341 ggaggaacag aagcatgagc tgcgcgacgg tgaacagaac gggaatccca taggaaacca
     2401 cgaaaagcac gatgaccatc accgccagca gataccagcg ccgggagatg aacagggatt
     2461 tataaaatcg cgacaaaaat gacatgggtc ttgttttagc gcgggacttc aatagaacgg
     2521 atgatccccg cgatcacctc atccgcggaa gctccttcca tttccttttc cggcgtcagg
     2581 accagccggt gccggagtac ggccggcgcc gtgtacacaa tatcttccgg cgtgatgaaa
     2641 tcgcggccgc gcatcgcagc gatcgctttg cttccgttaa ggatgccgat tgacgcccgt
     2701 ggggatgcgc cgagatatac tgatttgtga tttcgcgtcg ctcctacgat cgcggtaata
     2761 aattggatca acttctcttc gacatgcacg ccccgtacct tggtccggag gtcccctatc
     2821 cgctccgcag tcagcacgga ttgtaccgtg tcgatccgta cgtccccgcg caggcggtga
     2881 tggttcgcca cgatatggaa ttcttcttcc gaactcgggt aaagcacgtc gatcttgaag
     2941 aggaaccggt cgagctgtgc ctccggcagg tggtaggtcc cctcctgctc gatgggattc
     3001 tgcgtcgcga ccaccatgaa aggcgggtgc aagggatagg tttgtccgtc taccgttacc
     3061 tgccgctctt ccatcacctc gaacaaggcc gactgggtct tggccggcgc ccggttgatc
     3121 tcgtcgatca ggacgatatt gctgaaaata ggtcccggct tgaactgaaa ggcgctttcc
     3181 cggggattga acacggaagt gccgatcacg tcactcggca tgaggtcggg cgtaaactgg
     3241 atccgggaaa accctaccga gatcaccttg ctgagcaatt tcgccgtcag cgtcttggcg
     3301 acgcccggca ccccttcaat gaggatgtgc ccgtcggcaa ggataccggc gagcaacagc
     3361 tctatcatct gctcctgccc cacgatcacc ttccctatct gcgccctcag ttcctgcacg
     3421 gctcgttgca aatcctccag atccgtcctt tgttcaaaga gattctcttc catgttatgc
     3481 gattttatag aatgcttcca attgat
//
LOCUS       DATGKE010000739         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1195836, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000739 DATGKE010000000
VERSION     DATGKE010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195836"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VKU83_12535"
     CDS             <1..556
                     /locus_tag="VKU83_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HLI94439.1"
                     /translation="GFHWYETWTGNPMQFSNVGLVNQAFPKTHLLFTEGCKESFNFDS
                     LNSWSLGERYGYSMVHDFNSGAAGWIDWNILLDEHGGPNHVGNYCFAPVHADTRTGVL
                     IYTNAYYYIGHFSKFIHAGAKRIAVSTNRPDLQSTGFLNPDGSIVVVVLNTTDKPLPY
                     HLMWKGMAATTESLAHSIATIIIK"
     gene            complement(673..2448)
                     /locus_tag="VKU83_12540"
     CDS             complement(673..2448)
                     /locus_tag="VKU83_12540"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HLI94440.1"
                     /translation="MTLRFRCLVIPLLIAWAGPGEAQVSPDPYVPCQEMPTLISDYHA
                     DSRAIVRFYTSSFYGTRFRGYSLEGTSPETRARMGILDRQYLDKLNKVDFNGLPQECK
                     ADYILFSRDLKEDLRLLAVDSERYQKIRTWFPFADSIYALEKRRRRGHQLDAPNVARE
                     WSREAAEVNLLRARLKTDSSLDLDLLYEAGLVVSDLRRAIGSIYEFYQGYDPLFTWWV
                     PVNYRLLNDALSGYGDELKAKRTRFVANDKSGIVGRPVGRAELIRQLQYEMIPYTPEE
                     LIAIANKEFAWCDKEMLKASREMGFGDNWKAALEKVKNSYVTPGQQPGMILGLYNESV
                     DFIKKHDLVTIPPLAEETWGMVMMTPERQRVNPFFTGGDEISISYPTDSMSEEDRMMS
                     MRGNNPHFSRATVHHELIAGHNLQAFMNQRYRTYRDFGSGFWTEGWSLYWELLLWDLK
                     FPQTPEDKMGMLFWHMHRCARIIFSLNYHLGNWTPEQCIDFLVDRVGHERANAEGEVR
                     RSFATDYPPLYQLAYLTGGRQFYALKTELVDRGKMTYRQFHDTILKLNAMPVEMIRAI
                     LEAQPLDKDYRAHWRFYDGMKLPGD"
     gene            complement(2453..>4143)
                     /locus_tag="VKU83_12545"
     CDS             complement(2453..>4143)
                     /locus_tag="VKU83_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HLI94441.1"
                     /translation="AMSEALMVDPRGDTAIIHAQDKMKTTLERWIPIILAAQEPDGYL
                     QTAWTLRDSSKWRSRWSPENRGNHEGYTAGYFIESAINHYTLTEGRDRRLYNAAKRLA
                     DCWVAHLGPGKEVWFDGHQEMEQALVRFGRFVNDTEGQGHGDAYIRLAKFLLDSRGGG
                     DEYDQSQSPVQQQYEAVGHAVRATYTYSGMADIAAEMHDVDYQSAVMSLWDNMINRKY
                     YVTGGIGSGETSEGFGPDYSLRNNAYCESCSSCGLIFFEYKMNLAYHDARYADLYEET
                     LYNALLGSLNLAGTDFYYTNPLATAQPRYAWHSCPCCVGNIPRTLLMMPTWTYVRGDD
                     GIYVNLFVGSRMNVGKVAGTELRMVQKTDYPWSPAISIELEPGQEKTFTVYVRVPNRR
                     TSQLYTETPSVNGLTSIAVNGHRISPAIVRGYAVIRRSWKKGDRIELELPMAIQKIEA
                     DPRVKADSGRVAFRYGPLLYNVETADNDNIGQRMGKGPMRLEWKGNLLDGVMTINGVW
                     ADGTPLVAIPNYARMNRVPPTPTPRSDYSLRPPAGNEPPEYVPRAPVSLIWIKD"
BASE COUNT          834 a         1249 c         1127 g          933 t
ORIGIN      
        1 cgggttccac tggtatgaga cctggacggg caaccccatg caattcagca atgtcggtct
       61 ggtaaaccaa gcctttccga aaacacacct gctattcacg gaaggttgca aggaatcctt
      121 caatttcgac agcctgaatt cctggtcact gggtgaacgc tatggctatt cgatggtcca
      181 cgatttcaac agcggggcag caggctggat cgactggaat atcctgctcg acgaacacgg
      241 cggcccgaac catgtcggta attattgttt tgcgcccgta cacgcggaca cgcggaccgg
      301 ggtgctgatc tatacgaatg cgtattatta tatcggtcat ttttcaaagt tcatccatgc
      361 tggcgccaaa cggatcgcgg tgtccaccaa ccgccccgac ctgcagagca ccggattcct
      421 gaatcccgac ggcagcattg ttgtggtcgt cctgaataca acggacaagc ccttgcctta
      481 tcacctgatg tggaagggca tggcggccac gacggagagc ctggcgcatt ctatcgctac
      541 gatcatcatt aaatagggtc agggtttggg cggctgcgat gcctcatgcc gccgtggctc
      601 gctccggggg tgcttcgctc cggggatgcc tcgctccgct gtggcttcgc tccgccattc
      661 gcagggccgc ctttagtctc ccggcaactt catgccgtcg tagaacctcc agtgcgcccg
      721 gtagtcctta tccagcggct gtgcctccag gatggcgcgg atcatctcga ccggcatggc
      781 attcaatttt aagatcgtat cgtggaactg ccggtaagtc attttacccc tatccacaag
      841 ctctgttttc agcgcgtaaa actgcctgcc gcctgtcagg tacgcgagct gatacaacgg
      901 cggatagtcg gtggcaaacg atcgcctgac ttcaccttct gcgttggccc tttcgtgacc
      961 gaccctgtcc acgagaaaat cgatacattg ctccggcgtc cagttcccga ggtggtaatt
     1021 cagcgagaag atgatccgcg cgcaacggtg catatgccag aagagcatgc ccatcttgtc
     1081 ctccggggtt tgcgggaact tcaggtccca aagcaacagc tcccagtaaa gcgaccagcc
     1141 ttccgtccaa aagccggagc cgaaatcccg gtacgtgcga taccgctgat tcataaaagc
     1201 ctgcagatta tgcccggcga tcaactcgtg atgcaccgtg gcgcgcgaaa aatgggggtt
     1261 attcccccgc atgctcatca tccggtcctc ctccgacatg gaatcggtag gataggagat
     1321 actgatctcg tcgccgccgg tgaagaaggg attgacccgc tgcctttccg gcgtcatcat
     1381 gaccatgccc caggtctcct cagccagcgg cgggatcgtc acgaggtcat gcttttttat
     1441 gaagtctacc gactcgttat agagtcccag gatcatcccg ggctgttggc cgggagtcac
     1501 atatgaattc ttcactttct cgagagcagc cttccaattg tcgccgaatc ccatttcccg
     1561 cgatgctttc agcatttcct tgtcgcacca tgcaaactcc ttgttggcta tcgctatcaa
     1621 ttcctccggc gtgtaaggga tcatttcata ttgcagttgg cggatcagtt ctgcgcggcc
     1681 gacgggccgg ccaacgatcc cgctcttgtc attcgccacg aaccgcgttc gcttcgcttt
     1741 cagttcatcc ccgtagcccg acagcgcatc gttcaatagc cggtaattca ccggcaccca
     1801 ccacgtgaaa agcggatcat aaccctgata aaactcgtag atgctgccga tggcacgacg
     1861 gagatcgctg acgacaagcc ccgcttcata caagaggtcg aggtcgaggc tgctgtccgt
     1921 ttttaaccgc gcgcgcagca ggttgacctc ggccgcctcc cgggaccatt cgcgggcgac
     1981 gtttggcgca tcgagctggt ggccgcgccg tcgccgtttt tccaatgcat atatcgagtc
     2041 ggcaaacggg aaccaggtcc tgatcttttg gtagcgttcc gagtcgaccg ccagcagccg
     2101 gaggtcttcc ttcagatccc tgctaaaaag gatatagtcg gccttacatt cctggggcag
     2161 gccattgaaa tccactttat ttagtttatc caggtattgc cggtcaagga tgcccatcct
     2221 cgcccgggtt tccggcgaag ttccctcaag gctgtatccc cgaaagcggg tgccgtaaaa
     2281 gctactggtg tagaacctga cgatggccct gctgtccgcg tggtagtcgc tgatcagggt
     2341 cggcatttcc tggcaaggaa catacggatc gggtgatact tgtgcttccc ctggtccggc
     2401 ccaggcgatc aggagcggga taacgaggca tcgaaagcgg agcgtcatat cgttagtctt
     2461 ttatccagat gagtgagacc ggcgccctgg gaacgtattc cggcggttca ttgccggccg
     2521 gcggccgcag gctgtagtcg gaccgcgggg tcggggtggg agggaccctg ttcatgcgcg
     2581 cataattagg aatggcgacc agcggtgtcc cgtccgccca gacaccgttg atggtcatga
     2641 ccccatccag cagattgcct ttccactcca accgcattgg ccccttgccc atccgctggc
     2701 cgatattatc gttatcggcg gtctctacgt tgtacagcag cggcccgtaa cggaaggcca
     2761 cgcggccgct atccgccttc acgcgcggat cggcctcgat tttctgaatc gccatcggca
     2821 actcgagttc gatccggtcg ccttttttcc aggaccgccg gataacggca taaccccgaa
     2881 cgatcgcggg gctgatccga tgcccgttaa cggctatcga ggtcaaccca ttgaccgatg
     2941 gagtctccgt ataaagctgg ctggtccggc gattcggcac ccgcacgtat actgtgaatg
     3001 tcttctcctg ccccggctcc aattcgatgg aaatcgccgg cgaccaggga taatccgttt
     3061 tttgaaccat ccgcagttcc gtaccggcta ctttaccgac attcatcctg ctgccgacaa
     3121 acaaattgac atagatcccg tcatcacccc ggacataggt ccaggttggc atcatcagca
     3181 gcgtccgcgg gatattcccg acgcaacagg ggcaggaatg ccaggcatag cgaggttggg
     3241 ccgtcgccag cggattggta taataaaaat cggtgccggc gaggttcagc gatccaagca
     3301 aagcattgta caaggtttcc tcgtaaaggt cggcgtatct cgcatcgtga taggccaggt
     3361 tcatcttgta ttcgaaaaaa atcaggccgc agctggaaca ggactcgcaa tacgcattgt
     3421 tccggagtga atagtcggga ccgaaacctt ccgaagtctc accgcttccg ataccaccgg
     3481 tcacatagta cttacggttg atcatgttgt cccaaagcga catcacagcg ctctggtaat
     3541 cgacatcgtg catttcggcg gcgatatccg ccattcccga gtaagtatag gtggctctca
     3601 ccgcgtggcc gaccgcttca tattgctgct ggaccggcga ttggctctgg tcgtattcgt
     3661 cgccgcctcc gcggctgtcc aggaggaatt tggcaagccg gatataggca tccccatggc
     3721 cctgcccctc cgtgtcattg acaaaccggc caaagcggac cagcgcctgc tccatttcct
     3781 ggtggccgtc gaaccagacc tctttgcccg gcccgagatg cgcgacccag caatcggcca
     3841 gccgttttgc cgcattatac agcctacggt cgcggccttc cgtcagggtg taatgattga
     3901 tcgccgattc gataaaatag ccggccgtgt acccttcgtg gttcccccgg ttctcgggag
     3961 accaccggct acgccatttg ctgctgtccc gcagcgtcca cgccgtttgc agataaccgt
     4021 cgggctcctg tgcggcaagg atgatcggga tccaacgctc cagcgtcgtc ttcatcttgt
     4081 cctgcgcatg gattatggcg gtgtcacccc ggggatcaac catcagggcc tccgacatgg
     4141 cct
//
LOCUS       DATGKE010000740         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_1136450, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000740 DATGKE010000000
VERSION     DATGKE010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136450"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VKU83_12550"
     CDS             <1..744
                     /locus_tag="VKU83_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI94442.1"
                     /translation="VFGWPAIHTRMSNPDSKEPQPKVDSLFLDCGARNKKEVEELGIH
                     IGSVVTYQDGFKELAYDYLIGRAFDNRIGGFMIAEVARLLKENRKALPFGLYVVNAVQ
                     EEVGLRGAEMIARRIRPDVAIITDVTHDTTTPMINKIIEGDIACGKGPSLAFGPAVHN
                     KLLGIVQETAENKKIPVQMRAVSRSTGTDTDSFAYANDGCPSVLVSIPLRYMHTTVEM
                     LHKSDIEQTIRLMYETLLVLTPKTNLRYL"
     gene            744..893
                     /locus_tag="VKU83_12555"
     CDS             744..893
                     /locus_tag="VKU83_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94443.1"
                     /translation="MSASLLYIDPGSTSYLVQVIIAAILGAAFWIRKFWRKIRSFFTR
                     EKNDP"
     gene            890..2275
                     /locus_tag="VKU83_12560"
     CDS             890..2275
                     /locus_tag="VKU83_12560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLI94444.1"
                     /translation="MSSPATDSQIHPASYRDPAGFVFTAGGTLYRQVNRGYAGAYDRL
                     MQSGLYDKLAGEGLLIPHEETTSGLACTPEAYKILHPRRVCTVSYPSEWSPTQLRDAA
                     LLTLRIQSLAMEHGMSLKDATPFNVLFDKAKPVFIDTLSFEVYDASKPWVGYRQFCEC
                     FLFPLYLHYYSGLGTHKVAMAWPEGIAAGTTARLLPWKSHWSAGAWMHVHLQARMAAR
                     GGAGDGKGFGFSQVKLMRLIGHLQSLVAGLIPEARGHSKWSNYYDETIPSTEYLRSKE
                     QVFREYLGDMSFGDALDLGSNEGWFARIVAEKGKPVIAVDADWSCIDRLYRKGTAEIL
                     ALCVDLADPTPASGFGGAERDSFTSRAASDLVIALALVHHLAIADNVPLPLIAGYLSE
                     LTRSKLIIEFVPASDSKAQVLAARKDKALLEGYDAAHFETAFARHFRLEKTDPVPGTD
                     RTLYLMTKLQR"
     gene            2272..>2966
                     /locus_tag="VKU83_12565"
     CDS             2272..>2966
                     /locus_tag="VKU83_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94445.1"
                     /translation="MKKTLQTIPFFIPAIPVFFVLHGYRENLGYIHPRDAILLAGIYV
                     LASLLTFGIFYLIYRHATKAALAAGLILGFYFFFGALHDFLKAHCHAVSRYAIVLPAF
                     LVTATVWFVWLRRTRRVLSPLVFYLNLLFLIYLVIDGTAVGMPLHRRAAIGGEARAPH
                     DSIQLTPYTGGVKPDIYFLVFDAYSSSLALRDYYHFDNGDFDRFLTSRGFHILPASMS
                     NYKYTILSIPSIL"
BASE COUNT          589 a          952 c          777 g          648 t
ORIGIN      
        1 gtctttggct ggccggcaat ccatacgcgg atgagcaacc ccgacagcaa ggagccgcag
       61 ccaaaggtgg acagcctttt tctcgattgc ggagcccgca ataaaaagga agtcgaagag
      121 ctggggatcc atatcggctc cgtcgtcacc taccaggacg gctttaagga attggcttat
      181 gattacctca tcggccgtgc cttcgataac cggatcggcg gtttcatgat cgcggaagtg
      241 gcccggttgc taaaggaaaa caggaaagcc ctgcccttcg gcctgtatgt cgtcaatgcc
      301 gtccaggaag aggtaggcct tcgcggtgcg gaaatgatcg cgcgacggat caggccggac
      361 gtcgccatca tcaccgacgt cacccatgac accaccacgc ccatgatcaa caagatcata
      421 gagggcgata tcgcctgcgg caaaggacct tcgctggcct ttggaccggc agttcacaac
      481 aagttgctcg gcatcgtgca ggaaacggcc gaaaacaaga agatcccggt ccagatgcgg
      541 gcggtgtccc ggagcaccgg caccgacacc gattccttcg cgtatgcgaa cgacggttgt
      601 ccctccgttc tcgtttcgat cccccttcgc tatatgcaca ccacggttga gatgcttcat
      661 aagagcgata tcgagcagac gatccggctc atgtacgaga ccttgcttgt cctgacgccg
      721 aaaaccaacc tccgctatct ctgatgagtg cctccttgct ctatatcgat cccggcagca
      781 catcctatct cgtccaggtg atcattgccg ctattctcgg ggccgcattc tggatcagga
      841 agttttggcg gaaaatccgc tcgttcttca cccgcgaaaa gaacgatcca tgagttctcc
      901 cgctaccgac agccagatcc atcctgcatc ctatcgggac ccggcggggt ttgtgtttac
      961 cgccggcggg accctttacc ggcaggtgaa ccgcggctac gccggcgcct atgaccggtt
     1021 aatgcaatcg gggctctatg acaagctcgc cggtgaaggg ctcctgatcc ctcatgagga
     1081 aacgacttcc ggtctcgctt gcacgccgga agcctataag atacttcatc cccggcgcgt
     1141 ctgtaccgtt agctatcctt cggaatggag ccccacccag ctgcgggacg ccgcactgct
     1201 gaccctccgc attcagtccc tggcgatgga acacggcatg agcctgaagg atgcgacccc
     1261 gttcaatgtc cttttcgaca aggccaaacc cgtctttatc gatacccttt ccttcgaggt
     1321 atatgacgca tcgaaaccct gggtcggcta ccggcagttc tgcgaatgct ttctctttcc
     1381 gctgtatctt cattactatt ccgggctcgg cacccataag gtcgccatgg cctggcccga
     1441 aggaatcgct gccggtacaa cggcgcggct cctgccctgg aaaagtcatt ggagcgcggg
     1501 cgcctggatg cacgttcacc tccaggcccg gatggccgcc cgtggaggag ctggggacgg
     1561 gaaggggttc ggcttttcgc aagtaaagct gatgcgtctc ataggccatc ttcaaagcct
     1621 ggtcgccggt ttgatccctg aagcccgcgg ccattccaag tggagcaatt attacgacga
     1681 gacaatcccc agcacggaat acctccgaag taaggaacag gtgtttcgcg agtaccttgg
     1741 cgacatgagt ttcggggacg ccctcgacct tgggagcaac gaaggctggt ttgcgaggat
     1801 cgtggcggaa aagggcaaac ccgtcattgc ggtagacgcg gactggagct gcatcgacag
     1861 gctctaccgg aaaggtacag ccgagattct cgcgctgtgc gtcgacctgg ccgacccgac
     1921 gccggcctcc ggcttcggcg gagccgaacg cgactcgttt acgtcgcgcg ctgcatccga
     1981 cctcgtgatc gccctcgccc tcgtccacca tctggcgatc gcggataatg tgcccctccc
     2041 cctgatcgcc ggctacctga gcgaactcac ccgctccaaa ctgatcatcg aattcgtccc
     2101 ggcgtcggac agtaaggcgc aggtcctggc tgcccgcaaa gacaaagcgc tgctcgaagg
     2161 ctatgacgcg gcgcatttcg aaaccgcctt tgcccgccat ttccgcctcg aaaaaaccga
     2221 ccccgtcccc ggtaccgaca ggacactcta cctaatgacg aagctccagc gatgaaaaaa
     2281 acgcttcaaa ccatcccctt tttcattccg gcgattccgg tattctttgt cctccatggc
     2341 taccgtgaaa accttggcta tatccatccc cgcgacgcca ttctgctggc cgggatctat
     2401 gtcctggcct ccctcctgac cttcggcatt ttttatctta tctaccgcca cgccacaaaa
     2461 gccgcccttg ccgccggcct catcctggga ttttattttt ttttcggcgc cctgcatgat
     2521 tttctcaaag cgcattgtca cgccgtctcc cgctacgcca tcgtcctgcc ggccttcctt
     2581 gtgaccgcga ccgtttggtt cgtctggctt cgccggacac gccgtgtgct ctcacccctc
     2641 gtcttctacc tcaatctgct tttcctgatc tacctcgtga tcgacggaac cgccgtcggg
     2701 atgcccctgc atcgacgcgc cgccatcggg ggggaggcgc gggcgcccca cgactcgatc
     2761 caacttacgc cgtatacggg cggggtgaag cccgatatct attttctcgt cttcgacgct
     2821 tattcgagct cgctggcgct ccgcgactat tatcattttg acaatggcga tttcgaccgt
     2881 ttcctgacct cccgcggctt tcatatcctg ccggcaagca tgagcaatta taagtacacc
     2941 atactttcca ttccatcgat cctgaa
//
LOCUS       DATGKE010000741         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_947070, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000741 DATGKE010000000
VERSION     DATGKE010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947070"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VKU83_12570"
     CDS             complement(<1..774)
                     /locus_tag="VKU83_12570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLI94446.1"
                     /translation="MTKRTALLVLLAGGLSWAQAQTTIVRDPEIEGMVKEVNPDSLRS
                     YVKTMVAFGTRSTVSSVTDKKKGVGAARNWVLSKFNEWAAGSGGRLTAVVDTTTYVAD
                     GRRVKSNINLGNVVATLKGTDPNDNRIFIISGHLDSRRTDVMDGTNDAPGANDDGSGS
                     AAVLECARIMSKHSYPATIVFVTVSGEEQGLLGSTYMAKKAKAAGWNIEAVLNNDIMG
                     SNNSNETNVIDNTRIRVFSEGLAFIDTGRLAARIRQLGLE"
     gene            918..4064
                     /locus_tag="VKU83_12575"
     CDS             918..4064
                     /locus_tag="VKU83_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HLI94447.1"
                     /translation="MRALLLAAVVVLFACLQGSAQSTITVTGKITDEKGAAIVGATIT
                     EKGTRNATTTHDDGTWSLQVKPKTKLVISYVGYESTEIEARPGLTIALAPDVKSLSDV
                     VVTGVGVATNRKKVPIDVASVTSKDFAPSVTTNVQQALDGQIAGASIQQTSGTPGAGF
                     NIILRAVNSLDNSNPLIMVDGVQMNNINNIDPSLVDHIEVVKGPAGGMLYGAQGANGV
                     IQIFTKKGSLNGKMSINFNSKASIDNILTGKHSLLSTHHHYVTDASNNLLDGSGKLVA
                     MDATGIWSDPQVPIATAPANAFLTNDKTYNLPIYDHLKQGFRQALTFTNSVSVTGGTQ
                     TSDYAITASQLNQQDVLSNAFSRTNFAANIGLHPFRGFTFRTITQGIVGYQNLLSGNR
                     FNMLTNYNFVDFNWKDSTGHYPLKTVNSSGGYNTLSENQYHHQNHSTLEIFEDLDFNY
                     KFPRFLELDVKYGLDYNGDDFTNYYQNQTANLQYIKFNTYWGPSGQGALLDENSRTFF
                     QNGLYSAFVRTDWAKDFHSDLPITTTTEFAYDYRKTNFRQYYAEGIGLPVYPPPSISG
                     ATTKTAGDSYSTAITYGFLVNQTIDWGNLFGISAGVRSDYGSAFGAAYNAATFPRGTV
                     YFRPSELLTGQQDWLKDWKIRAAYGAAGVQPNPYDRQITLTPVTLGTGVAIALPSQNT
                     NDSLKLAVNYELEIGTDFTISPFNGEWLSRLVFSGSYWNRKTQDVYQNAQVAPSTGYA
                     TRLDNLSTITSHGVDLSLDASVYNGRIVTWDLSARWGFGRAIVNKIANGQDIVDFAFA
                     LKQGKPLGLFYVQTPLHSVGQLGPDGKTPLIPVANQGNYSLTSTGVVVLNSDNYVQYT
                     PTSNLSVIGHAYPDFTSSLTNRFTFFRKLSVSFQFDWVYGNSIYNVTKQWLYRPTGGT
                     GGQGGESIDLDKKLTIQGQTGSFVNYYNSIYNVGLPVSPFIENGSYIRLRDLSIGYDL
                     SRYVVGTRAIKRLTVTASGRNLLTFTKYSGLDPENVGAYDENGNNLSGQRIGAFTGVD
                     FYSTPNLRSYQFSLNVGF"
     gene            4089..>4781
                     /locus_tag="VKU83_12580"
     CDS             4089..>4781
                     /locus_tag="VKU83_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLI94448.1"
                     /translation="MNKQLYSLRSRKRTLLLVLASTLLLGACKKTILQLPNPNAPTPN
                     SSLVTEGGIDAFAQGIFYKWIAFETGDGNLNFFDIAWYVESNMGDEDFTPYSNYGSRY
                     PMNIASITLPPPYNTVIKNPSGFATQLDILRSFNTRAAGDGNTIQYEWDCFYYVNAQA
                     NALLLALDNTALKLSGDAATKKKLLQAWAYYWKGYAYSKLGSMYLAATIDDSPDSTAK
                     GLTSNNFIAHDQV"
BASE COUNT         1122 a         1485 c         1089 g         1085 t
ORIGIN      
        1 ctccagccct aactggcgta tccgcgcagc cagcctgccc gtgtcgataa aggccagccc
       61 ttcgctgaaa actcgtatcc gggtgttgtc gatgacattc gtctcgttgc tgttattgct
      121 ccccataata tcgttgttca gcaccgcctc gatattccag cccgccgctt tcgccttttt
      181 ggccatatac gtggaaccaa gtagtccctg ctcttcgccg ctcacggtta caaaaacgat
      241 cgtcgccgga tagctgtgtt tgctcatgat ccgcgcacat tccagcaccg cggcactccc
      301 gcttccgtcg tcattagccc ccggcgcgtc attggtcccg tccatgacat ccgtcctccg
      361 gctgtccaaa tgcccgctta taataaagat ccggttatcg ttgggatcgg taccctttag
      421 cgtggccact acattcccca ggttgatatt cgacttgacc cgccggccat ctgccacata
      481 ggtagtggtg tctaccaccg ccgtcaaccg cccgccactg cccgccgccc attcattgaa
      541 cttggacagc acccaattac gcgccgcgcc gacccccttt ttcttgtccg ttacggagct
      601 tacggtgctc cgggtgccga aagcaaccat tgtcttgaca tacgaccgca gcgaatcggg
      661 attcacctcc ttgaccatcc cctctatctc aggatcgcgc acgatagtgg tctgtgcctg
      721 ggcccagctt agcccgccgg ccagtaaaac aagtaatgca gttctttttg tcatgtctgg
      781 cagtttgcgg caaatttcat ttttttcctc aactacgtaa ataattagta ttcgcttatc
      841 gccattatta gtggtctccg caaacgcgat tcatggatat aaccctatat tagataaaca
      901 tcaaccaaga caacattatg agagccctgc tactagcggc tgtggtcgtc cttttcgctt
      961 gtttacaagg atcggcccag tcaactatta cggttaccgg taagatcacc gacgaaaaag
     1021 gcgccgccat cgttggcgcc acaatcacag aaaaaggcac ccgcaacgcc acaaccaccc
     1081 atgacgatgg cacctggtcg cttcaggtaa aacctaaaac aaaactcgtt atcagctatg
     1141 tcggctatga gtccaccgaa atagaagccc ggcccggcct cactatagcg ctcgctccgg
     1201 acgtcaaatc gctttccgat gtcgtcgtta ccggcgtggg cgtggctacc aaccgcaaaa
     1261 aggttcccat cgatgtcgct agcgtcacct cgaaagattt cgcccccagc gtgacaacca
     1321 acgtccagca ggccctggat gggcagatcg ccggtgcgag tatccaacag accagcggta
     1381 cacccggcgc gggtttcaac atcatcctga gggccgtcaa cagcctcgac aactcaaacc
     1441 cgcttatcat ggtcgatggg gtgcaaatga acaacattaa caacatcgac ccctccctcg
     1501 tcgaccatat agaggtcgtc aaaggtcccg ccggcggcat gctctacggt gcccagggcg
     1561 ccaatggcgt tatccagatc tttacgaaga aaggctcgct caatggcaaa atgagtatca
     1621 atttcaattc caaggccagc atagacaata tcctcaccgg caaacattcc ctcctgagca
     1681 cccatcatca ctacgttacc gatgccagta acaatctcct ggatgggagc ggtaaattag
     1741 tcgcaatgga tgcgaccggg atttggagcg atccccaggt gccgatcgct acagcccccg
     1801 ccaacgcctt cctgacgaac gacaagacgt ataacctccc gatctacgat cacttgaaac
     1861 aaggtttccg tcaggccctc accttcacca attccgttag cgttaccggc ggtacgcaga
     1921 cgtcggacta tgccatcacc gcttcccagc tcaatcagca ggacgtgctg agcaatgcat
     1981 ttagccgtac caactttgcg gccaacatcg gtctccatcc cttcagggga tttaccttcc
     2041 ggaccatcac gcagggtatc gtcggctacc agaacctcct cagtggcaac cggttcaata
     2101 tgctgaccaa ttacaatttc gtcgacttta actggaaaga ctctacaggc cactatccgc
     2161 tgaaaacggt caattcttcc ggcggctata ataccctttc ggaaaaccaa tatcaccacc
     2221 agaaccattc aacgctcgag atcttcgagg acctcgattt caattataaa ttcccgcgtt
     2281 tcctggagct cgatgtcaaa tacggactgg attacaacgg cgatgatttc accaattatt
     2341 atcagaacca gactgcgaat ctccaataca tcaaattcaa cacctattgg ggacctagcg
     2401 ggcaaggcgc ccttctggac gagaattcca ggaccttctt tcaaaacgga ctgtattccg
     2461 ccttcgtgcg taccgattgg gcaaaagatt tccactccga tctgcccata actacgacta
     2521 ccgagttcgc ctacgattac cgtaaaacca attttagaca atactatgcg gaaggtatcg
     2581 ggctccccgt ttatcctccg cccagcatca gcggggcgac gaccaagacc gcgggcgaca
     2641 gctactcgac cgctattacc tacggcttcc tggtcaacca gaccatcgat tggggtaacc
     2701 tcttcggaat ttccgccggc gtgcggtccg attacgggag tgccttcggc gccgcctaca
     2761 acgcagccac ctttcctcgc gggaccgtct atttccggcc atccgagctg ctgacaggcc
     2821 agcaggactg gctcaaggac tggaagatcc gcgcggcata tggcgcagca ggcgttcagc
     2881 ccaatcccta cgaccggcag atcacgttga ccccggtcac gctgggtacc ggcgttgcca
     2941 tagcccttcc gtctcaaaat acaaatgaca gtctgaaact ggcggtaaac tacgaattgg
     3001 agatcggtac cgatttcacc atttcgccat ttaacggtga atggctgtca cgattggtat
     3061 ttagcggtag ctattggaat cgcaaaaccc aggatgtcta ccagaatgct caggtagcgc
     3121 cttccaccgg ctacgccacc cggctcgata acctgagcac catcacgtcg cacggagtcg
     3181 acctttcgct cgacgcttct gtttacaacg gccgcatagt tacctgggat ctcagcgccc
     3241 gctggggctt tggccgcgct atcgtgaaca agatcgccaa tggccaggat atcgtcgact
     3301 tcgccttcgc gctcaaacaa ggcaagcccc tcggtttgtt ctacgtccag acccccttgc
     3361 attccgtggg ccagctcggg ccggatggca agacacccct cattcccgtt gccaaccagg
     3421 gaaattattc gctcaccagc actggcgtgg tggtgctcaa cagcgacaac tacgtacagt
     3481 atacgccaac cagcaatctt tccgttatag gccatgccta tccggacttc acctcctcgc
     3541 tgaccaaccg gttcacgttc ttcaggaagc tgagcgtttc cttccaattc gattgggtct
     3601 acggcaatag catctacaat gtcaccaaac aatggctcta ccggcccaca ggcggaacgg
     3661 gcggacaagg cggtgaatcc atcgatctgg acaagaaact cacgattcag ggccaaaccg
     3721 gatcgttcgt caactactac aactccattt acaacgtagg cttgcccgtg tcacccttca
     3781 tcgagaatgg ctcctatata cgcctgcgcg atctgtccat tggctacgat ctgtcccggt
     3841 acgtcgtcgg cacgagagcc attaaacggc tgaccgttac ggcttcaggt cgcaacctgt
     3901 tgacctttac caagtatagc ggtctcgatc cggagaatgt cggcgcctat gacgaaaatg
     3961 gcaacaacct ctctggtcaa aggataggag ctttcaccgg tgtcgacttt tacagcacgc
     4021 cgaacctccg gtcctatcag ttttcactga atgtcggatt ctaatcgatc acttcaatta
     4081 cgaataatat gaataagcaa ctatatagcc tacgctcccg gaaacggacc cttttgctgg
     4141 tactggcttc caccctgctg ctgggagcct gcaagaaaac catcctgcag ctgcccaatc
     4201 ccaatgcacc cactccgaac agctccctgg tcaccgaagg cggcatcgat gcctttgctc
     4261 agggcatttt ttacaaatgg atcgctttcg agacggggga tggcaatctc aacttcttcg
     4321 atatcgcctg gtacgtagaa agcaatatgg gcgatgagga tttcacgcct tattccaact
     4381 acggttcccg ttaccccatg aatatcgcca gcatcacctt gccgccgccc tataataccg
     4441 tcatcaagaa tccctccggc tttgccacac agctggatat cctccggtct ttcaacaccc
     4501 gtgccgccgg cgatggcaat accatccaat acgaatggga ctgcttttat tatgtcaatg
     4561 cacaggcgaa cgcgctgctc ctggcgttgg ataataccgc gcttaagctc tccggcgatg
     4621 ccgccaccaa gaaaaagctg ctccaggcct gggcctacta ttggaagggc tatgcctact
     4681 ctaaattggg ttcgatgtat ctggccgcca ccattgacga ttctcccgat agcaccgcca
     4741 aaggtctgac cagcaataac ttcatcgcac atgaccaggt g
//
LOCUS       DATGKE010000742         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U9851 k141_680210, whole genome
            shotgun sequence.
ACCESSION   DATGKE010000742 DATGKE010000000
VERSION     DATGKE010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965419
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 13:11:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,519
            CDSs (total)                      :: 2,498
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680210"
                     /isolate="SMAG_U9851"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1538
                     /locus_tag="VKU83_12585"
     CDS             180..1538
                     /locus_tag="VKU83_12585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLI94449.1"
                     /translation="MNLKNASILVIDDDPDVLTAVRLLLKTEVGDVVTEKNPENMRSI
                     LSKKSFDLILLDMNFNSSINTGNEGLFWLRKIKEFGSAAAVIMITAYGDIDLAVRSLK
                     EGASDFVVKPWHNERLISTIKEALKRNDPKKAGGSADGMDSVIGTELLGESEAMRDIF
                     FKVEKIAPTDANILILGENGTGKDLIAKAIHQHSLRAEKPYVKVDVGALTETLFESEL
                     FGHKKGAFTDAREDRMGRFEAGNGGTLFLDEIGNISLHQQAKLLSVLQNRQVTRLGSN
                     QPVPIDIRLICATNVPLAELANENRFRKDLIYRINTVEIMVPPLRKREDDILLLARHF
                     DRIYTNKYMKPQHDFDVRAIEKLKSYHFPGNVRELQYTIERAVIMAEGDVLQAKDLIF
                     SPIELAPPESDEPKELKLSTIEKNAILKVIEKHNGNISKAAKELGLTRTALYRRLSKY
                     DI"
     gene            1544..2887
                     /locus_tag="VKU83_12590"
     CDS             1544..2887
                     /locus_tag="VKU83_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLI94450.1"
                     /translation="MVFKRYQERILIRVIFLFSTLALAAYLLVRAEYPYLIFLLLVVT
                     LQLVDFYNFHRKAHLEVEQFVESIHYRDFSRYFDVKEAPVELQSLRQGFNEINSTFKL
                     ISREKETQYQYLQKILELVNTGILSYEHKSGEIGWMNEAFKNMLGVPYLKTIASLDRR
                     DPALYQEILNLRPGDNKIITLSKDKNVFKTLMAATAFQTDNRIYKLIAFQDVNEALDE
                     TEAKAWQKLLSVMTHEIMNSVAPISSLAETMLHRLQETSRMLEEQPGALEDLELGIGT
                     IRRRSEGLLKFAETYRNLNKITTLNLKRIFARDLFENLHHLMQPTLDQKHIELEILLI
                     DPDLSLEADTNLIEQVLINLVVNAIEAVKDREQPLITLSANAATNGRPVIKVADNGMG
                     MAPEVMENIFIPFFSTKKNGSGIGLSLCKQILLLHKGNIQVQSVEGVGTAFYLHF"
     gene            complement(2884..>3258)
                     /locus_tag="VKU83_12595"
     CDS             complement(2884..>3258)
                     /locus_tag="VKU83_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HLI94451.1"
                     /translation="LPDFAARPGRKRSRILVRKGAEFQTVRIEDACYFFTEHKLIFLV
                     DKENRKYMAEKSNLSELEEELDGHLFYRANRKYIINANYIKRFKPLEKSKISVELTLP
                     VNEEIIISQENSASFKKWIGES"
BASE COUNT          830 a          786 c          877 g          765 t
ORIGIN      
        1 atgatttaca atttattagg agtgaaaagc ccgatctata gttcattttt gatacaagaa
       61 ctgtccggtt ttgatacagt tcccgttttt atggattttg cataaaaaat taattgccat
      121 aagtttacat ttaaggtata cttgatgtat tcgaatgttg cattccaact gaaaatttta
      181 tgaatcttaa gaatgcctcc atattggtga ttgacgacga tccggacgtg ctgacggcgg
      241 tcagattatt actaaagacc gaggtggggg acgtcgttac ggagaaaaat ccggaaaata
      301 tgcggtcgat cctttcaaaa aagagtttcg atctcatcct gctggacatg aatttcaaca
      361 gctcgatcaa caccggcaac gaaggcttgt tctggctccg gaaaataaag gaattcgggt
      421 ctgcggctgc cgtcatcatg atcacggctt acggtgatat cgacttggca gtcaggtcgt
      481 tgaaggaggg ggcaagcgac ttcgtggtaa agccatggca caacgagcgg ttgatttcca
      541 cgattaagga agccttgaaa cggaatgatc ccaagaaggc cggcgggtcc gcggacggaa
      601 tggactcggt gatcgggacg gaacttttgg gtgaatcgga ggcaatgcgg gatattttct
      661 tcaaggtgga aaagatcgcg cctactgacg ccaatatctt gatccttggc gagaacggga
      721 cgggtaaaga tctgatagcc aaagcgatac atcagcattc gcttcgggcc gaaaaaccct
      781 acgtcaaggt cgatgtcggg gcgctcaccg aaaccctgtt tgagagcgag ctgttcggcc
      841 acaaaaaggg agcgtttaca gatgccaggg aggacagaat gggtcgtttt gaagccggca
      901 atggcggaac gctcttcctc gatgagatcg ggaatatcag cttgcatcag caggctaaac
      961 tattgtctgt cctgcaaaac aggcaggtga cgaggttggg ctccaatcaa cccgtgccga
     1021 ttgacatccg cctgatctgc gccacgaatg tgccgctggc ggaacttgca aacgaaaacc
     1081 ggttccggaa agacctcatt taccggatca acactgtcga gatcatggtg ccgccgctgc
     1141 gaaagcggga ggatgatatc cttttgctgg cacggcattt cgaccggatc tataccaata
     1201 aatatatgaa gccgcagcat gactttgatg ttcgggccat cgagaagctt aagtcctacc
     1261 attttcccgg caacgtccgg gagctccaat acaccatcga gcgggcggtt atcatggcgg
     1321 aaggggatgt gctgcaggca aaggacctga tattttcacc gatcgagctg gcgccgccgg
     1381 aatcggatga gccaaaggaa ctgaaattaa gcacaatcga gaagaatgcg atcctgaaag
     1441 ttatcgaaaa gcacaatggt aacatatcga aagctgcaaa ggaattgggc ctgacgcgga
     1501 cggcgctgta ccggagatta agtaaatatg atatatagag gccatggtct ttaaacgata
     1561 ccaggaacgg atattgatcc gggtcatctt cctgttttcc acgcttgccc tcgcggctta
     1621 tttgctggtc agggcagagt acccatacct gatctttctg ttgctggtgg taacccttca
     1681 gctggtcgac ttctataatt ttcaccgcaa ggcgcatctc gaggtcgagc agttcgtaga
     1741 atcgatccac taccgggatt tttcgcgata cttcgacgtc aaggaggccc cggtggagct
     1801 tcaatcgctc cggcagggct tcaacgagat caattccacg ttcaagctca tcagtcgcga
     1861 aaaggagacc cagtaccagt acctgcagaa gatcctcgag ctggtgaaca ccgggatctt
     1921 gtcgtacgag cataaaagcg gcgagatcgg ctggatgaac gaggcgttca agaatatgct
     1981 gggcgttccc tatctcaaga ccattgccag cctggaccgg cgggacccgg cgttatacca
     2041 ggaaatcctc aatttgcggc cgggggacaa taagataatt accctgtcga aggataaaaa
     2101 cgttttcaag accctgatgg ctgcgacggc gttccagacc gacaaccgga tctacaagct
     2161 cattgccttc caggatgtga atgaggcgct ggacgagacg gaggccaagg cctggcaaaa
     2221 attgctgagc gtcatgaccc atgagatcat gaattccgtg gcgccaatct cctcccttgc
     2281 ggagacgatg ctgcaccggt tacaggagac atcacggatg ctggaagagc agccgggcgc
     2341 cctcgaggac ctcgagctgg ggattggaac gatccgcagg cgaagcgaag gattattgaa
     2401 atttgccgag acttaccgca atttgaacaa gatcacgacc ctcaacctta agcggatctt
     2461 tgcccgcgac ctgttcgaaa acctgcatca tctgatgcag ccgactttgg atcaaaaaca
     2521 catcgagctg gagatcctcc tcatcgatcc ggacctttcc ctggaggcgg acaccaacct
     2581 gatcgagcag gtcctgatca atctggtcgt gaatgccatc gaggccgtga aagatcgcga
     2641 gcagccgctg atcaccttgt ccgcgaatgc ggcaacgaac ggccggccgg tcatcaaggt
     2701 agccgataac ggcatgggaa tggcgcccga agtaatggaa aatattttta ttcccttctt
     2761 tagtaccaag aagaacggga gcgggattgg attaagcttg tgcaagcaga tcctgctgct
     2821 tcataaggga aatatccagg tgcaatcggt tgaaggagtc ggcaccgcct tctacctgca
     2881 tttctagctt tcgccgatcc attttttgaa ggaagcggag ttttcctggc tgataatgat
     2941 ctcttcgttt accggcagtg tgagctctac gctgatcttg cttttctcga gcggtttgaa
     3001 tcgcttgata taattggcgt tgatgatata cttccggttt gcgcggtaaa acaggtggcc
     3061 atccaattcc tcttcgagct cgctgagatt gcttttttcc gccatatatt ttctgttctc
     3121 tttgtccacc agaaagatga gtttgtgttc cgtgaaaaaa tagcaggcat cctcgatccg
     3181 gacagtctgg aattcggccc ctttccggac caggatgcgg gagcgcttcc tgccgggtct
     3241 cgccgcgaaa tccggcaa
//
